LOCUS       DASUCY010000001         4645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_305258, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000001 DASUCY010000000
VERSION     DASUCY010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305258"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..107)
                     /locus_tag="VFV25_00005"
     CDS             complement(<1..107)
                     /locus_tag="VFV25_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY1391724.1"
                     /translation="MALHFDLVDLRLMVHVAEANSLTRGAEAAHLSVPAA"
     gene            complement(139..1413)
                     /locus_tag="VFV25_00010"
     CDS             complement(139..1413)
                     /locus_tag="VFV25_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HEY1391725.1"
                     /translation="MVHQTTKTKMSRAPSASRNNSSMMTMTSGSQAPRSTGRGAWAAI
                     LGPALAAVLTMQLTGCGTAPPAPVEPPPPAAAVSAPASAPALPPILRPASRWLPARWE
                     ELPGWGEDAAIQAWPALLRSCGKPAPGWEAACASALGLADPDETKVRAWLTSKLQPYR
                     IEALDGQAEGLMTGYFEPLVEASHKRTTRFSMPLYMPPSDYATRKPWWTRAEVRNHPE
                     ARTALRGHEIAWVADPLDALLLQIQGSGRLRFAGESISVRLAFAGHNDQPYKSIGRWL
                     VDQGAFTLEQANWPAIKAWARANPGRVQEMLDANPRFIFFRTEPLPDPTLGPLGAQGV
                     PLTPGRSIAVDKTAVPYGTPVWVATTEPQPWSRQPPPPRPLQRLMMAQDTGSAITGAL
                     RADYFWGWTEGAEDRAGRMKQPLRMWALWPRS"
     gene            1346..1492
                     /locus_tag="VFV25_00015"
     CDS             1346..1492
                     /locus_tag="VFV25_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1391726.1"
                     /translation="MLLLFLEALGALLIFVFVVWWTMFSGRKKGEPPSAALEQHKDDG
                     SSSS"
     gene            complement(1487..3529)
                     /locus_tag="VFV25_00020"
     CDS             complement(1487..3529)
                     /locus_tag="VFV25_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HEY1391727.1"
                     /translation="MLDKLRRGGRWDLTALLNAADPKASMPERHLWLIRLLEWLRRSG
                     PLEAADGESSPPAALRLRHLLNVLDRHPAHREHVVALLGQFWREVDVAALLADFGFAP
                     RTAFASELGERLRNRLLPGTPATTDLAELFGLLFSDPADADWLLALDETTLERLGSLF
                     DAANTAGGGAGGPLGWREPFYEAVMFLASQVRAAGFSSALRQRMSASTLSPQPFRQLA
                     RTAEGLRSLAEAGDTPGLLREMQYLRALLEACRQAAASVHDHLEEHGVSVGIVFQLDQ
                     LQARTHRIEELLNCAISPAPGHEIAHLLAGLVRTAAERRSVRALLARNFSMLARKLAE
                     RSAETGEHYITRDRSEYRAMLRKALGGGAIIGITTFLKFLVVAAGFSAFWSGWWAGIN
                     YAASFVLIHLLHWTVATKQPAMTAPAMAAKLDDIAGAGALEGFVDEVAHLIRSQVAGI
                     LGNLAAVFPLVLAAQGLSWLAFGRPLVSAQDAGHALHALTLLGPTAVYAAFTGVLLFV
                     ASLIAGWVENAFVLNKLDSAIRWNPRIVAALGHARAERWSRWWRANISGLSSNISLGL
                     MLGLVPAVLGFFALPLEVRHITLSTGQLAAALGTLGPELLRHTEFWWCAAALPVTGLL
                     NLGVSFWLAFRLALRSRGIRVNDRARIYAAIRARLRHRPLSFLLPPREAAAAAAAS"
     gene            complement(3559..3933)
                     /gene="apaG"
                     /locus_tag="VFV25_00025"
     CDS             complement(3559..3933)
                     /gene="apaG"
                     /locus_tag="VFV25_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HEY1391728.1"
                     /translation="MTKPEFAVSVATRYLPEESDPERERYAFAYTITIRNSGDVPAQL
                     IARHWLITDAAGGIQDVRGLGVVGHQPLLQPGERFEYSSWAVLPTPRGRMEGSYFCMT
                     EKAESFEVEIPAFELSLRAALH"
     gene            4069..>4645
                     /gene="rpe"
                     /locus_tag="VFV25_00030"
     CDS             4069..>4645
                     /gene="rpe"
                     /locus_tag="VFV25_00030"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEY1391729.1"
                     /translation="MPRNHRIAPSILSADFARLGEELRDVIAAGADWIHFDVMDNHYV
                     PNLTFGPMVCEALRKHALRPDGSRVPIDVHLMVQPVDALAQAFAKAGADLISFHPDAS
                     THVDRTLQLIKAEGCQAGLVFNPAEPIDVLEWVLDKLDLVLIMSVNPGFGGQSFIPGA
                     LRKIEQVRKLIDASGRDIRLEVDGGIKVDNIR"
BASE COUNT          709 a         1606 c         1680 g          650 t
ORIGIN      
        1 gccgccggca ccgacaggtg cgccgcctcg gcgccgcgcg tcaggctgtt agcttcggcg
       61 acatgcacca tcagtcgcag atcgacgaga tcgaaatgca gggccatggc gcgactgtat
      121 cgactgcggg acttgacact aggacctcgg ccacagcgcc cacatgcgca gcggctgctt
      181 catgcgcccg gcgcggtctt cggcgccttc ggtccagccc cagaagtagt cggcgcgcag
      241 ggcgccggtg atcgcgctgc cggtgtcctg ggccatcatc aggcgctgca gcggccgcgg
      301 cggcgggggc tggcgcgacc agggctgggg ctcggtggtg gcgacccaca ccggcgtgcc
      361 gtagggcacg gccgtcttgt ccaccgcgat cgagcggccc ggcgtcaacg gcacgccctg
      421 ggcgcccagc ggccccagcg tcgggtcggg cagcggctcg gtgcggaaga agatgaagcg
      481 tgggttggca tccagcatct cctgcacgcg gcccgggttg gcgcgtgccc aggccttgat
      541 cgcgggccag ttggcctgct ccagcgtgaa ggcgccctgg tcgaccagcc agcggccgat
      601 cgacttgtag ggctggtcgt tgtgcccggc gaaggccagg cgcacggaga tcgactcgcc
      661 ggcgaagcgt agccggcccg agccctggat ctgcagcagc agcgcgtcca gcggatcggc
      721 cacccaggcg atctcgtggc cgcgcagggc ggtgcgggcc tcggggtggt tgcgcacctc
      781 ggcgcgcgtc caccagggct tgcgcgtcgc gtagtcgctg ggcggcatgt agagcggcat
      841 cgaaaagcgc gttgtgcgct tgtgagaggc ctcgaccagg ggctcgaaat aaccggtcat
      901 caggccctcg gcctggccgt ccagggcctc gatgcgatag ggctgcagct tgcttgtgag
      961 ccaggcgcgc accttggtct cgtcggggtc ggcgaggccg agcgcactgg cgcaggccgc
     1021 ttcccaaccc ggggccggct tgccgcagct gcgcagcagc gcgggccagg cctggatcgc
     1081 ggcatcttcg ccccagccgg gcagttcttc ccagcgcgcc ggcagccagc gcgaggccgg
     1141 gcgcaggatt gggggcaggg ctggcgcgga agccggcgcc gacacggcgg cagccggcgg
     1201 cggcggctcg acgggtgcgg gcggcgcggt cccgcagccc gtcagctgca tcgtcagcac
     1261 ggcggcgagc gccgggccta agatcgcggc ccatgctccg cggcctgtgc tgcggggcgc
     1321 ttgcgatccg gaagtcatcg tcatcatgct gctgttgttt ctcgaagcgc taggcgctct
     1381 gctcatcttc gtcttcgtgg tctggtggac catgttctcg ggccgcaaga agggcgagcc
     1441 gccctcggcc gcgctcgaac agcacaagga cgacggatca tcgtcttcat gatgcagcgg
     1501 cggctgccgc ggcctcgcgc ggcggcagca ggaagctcag cggccggtgc cgcaggcgcg
     1561 cgcgtatcgc ggcgtagatg cgggcacggt cgttcacgcg tatgccgcgc gaacgcagcg
     1621 ccagcctgaa ggcgagccag aagctcacgc ccaggttcag caggccggtg acgggcagcg
     1681 ccgccgcgca ccaccagaac tcggtgtggc gcagcagctc tgggcccagc gtgcccaggg
     1741 cggcggccag ctggccggtg gacagcgtga tgtggcgcac ctccagcggc agcgcgaaga
     1801 agcccagcac cgccggcacc aggcccagca tcaggcccag cgagatgttg gagctcaggc
     1861 ccgagatgtt ggcgcgccac cagcgcgacc agcgctcggc gcgcgcatgg cccagcgcgg
     1921 cgacgatgcg cgggttccag cggatcgcgc tgtcgagctt gttcagcacg aaggcgttct
     1981 cgacccagcc ggcgatcagg ctagcgacga acagcagcac gccggtgaag gccgcgtaga
     2041 cggccgtcgg gccgagcagc gtgagcgcgt gcagcgcgtg cccggcatcc tgcgcgctca
     2101 ccagcggacg gccgaaggcg agccagctca ggccctgtgc agccagcacg agcgggaaga
     2161 ccgcggccag gttgcccagg atgccggcca cctgcgagcg gatcaggtgg gcgacctcgt
     2221 cgacgaatcc ctccagcgcc ccagcaccag cgatgtcgtc cagcttggcc gccatcgcgg
     2281 gcgcggtcat ggccggctgc ttggtcgcca ccgtccagtg cagcaggtgg atcagcacga
     2341 agctggccgc gtagttgatg ccggcccacc agccgctcca gaaggccgag aagccggccg
     2401 ccaccaccag gaatttgagg aaagtcgtga tcccgatgat ggcgccgccg cccagggcct
     2461 tgcgcagcat ggcgcggtat tcgctgcggt cgcgggtgat gtagtgctcg ccggtttcgg
     2521 cgctgcgctc ggccagcttg cgcgccagca tcgagaagtt gcgcgccagc agcgcgcgca
     2581 cgctgcggcg ctcggcagct gtgcgcacca ggccggcgag caggtgggcg atctcgtgcc
     2641 ccggcgcggg cgagatcgcg cagttgagca attcctcgat gcgatgcgtg cgcgcctgga
     2701 gctggtcgag ctgaaacacg atgcccaccg acacgccgtg ctcctccagg tgatcgtgca
     2761 cgctggccgc ggcctggcgg caggcttcga gcagggcgcg caggtactgc atctcgcgca
     2821 gcaggcccgg cgtgtcgccg gcctcggcca gcgagcgcag gccttcggcg gtgcgtgcca
     2881 gctggcggaa gggttgcggg ctcagcgtcg acgcgctcat gcgttggcgc agggcgctcg
     2941 aaaagccggc ggcgcgcacc tgcgaggcca ggaacatcac ggcctcgtag aagggttcgc
     3001 gccagccgag cggaccgccc gcgccgccgc ccgcggtgtt ggccgcgtcg aacagcgagc
     3061 ccagccgctc cagcgtggtc tcgtccagcg cgagcagcca gtcggcatcg gcggggtcgg
     3121 agaacagcag gccgaagagc tcggccaggt cggtggtggc cggcgtcccg ggcagcagcc
     3181 ggttgcgcag gcgttcgccc agctcgctgg caaaggccgt gcggggtgcg aagccgaaat
     3241 cggccagcag cgcggccacg tcgacctcgc gccagaactg gcccagcagc gcgaccacat
     3301 gctcgcgatg cgcggggtgg cgatccagca cattgagcag atggcgcagg cgcagcgcgg
     3361 ccggtggcga ggattcgccg tccgcggcct ccagcgggcc gctgcggcgc agccactcca
     3421 gcaggcggat cagccacaga tggcgctcgg gcatcgacgc cttggggtcg gctgcgttca
     3481 gcagggccgt gagatcccag cgcccgccgc ggcggagctt gtcgagcaat cctcccatcg
     3541 cgtcggctgg gcctggcctc agtgcaaggc tgcccgcagg ctcagctcga aggcggggat
     3601 ctcgacctcg aaggactcgg ccttctccgt catgcagaaa tagctgcctt ccatgcgccc
     3661 acgcggcgtc ggcagcacgg cccagctcga gtactcgaag cgctcgcccg gctgcagcag
     3721 cggctgatgg cccaccaccc ccaggccgcg cacgtcctgg atgccgccgg ccgcatcggt
     3781 gatcagccaa tgccgcgcga tcagctgggc cggcacgtcg ccgctgttgc gtatggtgat
     3841 ggtgtaggcg aaggcatagc gctcgcgctc gggatcggat tcctcgggca ggtagcgggt
     3901 cgcgacgctg acggcgaatt cgggcttggt catgagggca ttctaggaag cctctgtggc
     3961 aacgccgaac aagaccctcg tggcgcgcgc atccacgggg gacttgttca gtggcgccct
     4021 aaaatccacc gcccagcact ccccttcgcg ccaggcgcaa agcccatcat gccccgcaac
     4081 caccgcatcg cccccagcat cctctcggcc gatttcgcgc gcctgggcga ggagctgcga
     4141 gacgtcatcg cggccggcgc cgattggatc catttcgacg tgatggacaa ccattacgtg
     4201 cccaacctca ctttcggtcc catggtctgc gaggcgctgc gcaagcacgc gctcaggccc
     4261 gacggcagcc gcgtgcccat cgacgtgcac ctgatggtgc agccggtgga tgcgctggcc
     4321 caggccttcg ccaaggccgg ggccgacctg atcagcttcc accccgatgc gagcacccac
     4381 gtggaccgca cgctgcagct gatcaaggcc gaaggttgcc aggccggcct cgtgtttaac
     4441 ccggccgagc ccatcgacgt gctggaatgg gtgctcgaca agctcgacct cgtgctcatc
     4501 atgagcgtga acccgggctt cggcgggcag agcttcatcc ccggcgcgct caggaagatc
     4561 gagcaggtgc gcaagctcat cgatgcgagc gggcgcgaca tccgcctgga agtcgacggc
     4621 ggcatcaagg tcgacaacat ccgcc
//
LOCUS       DASUCY010000002         2220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1526209, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000002 DASUCY010000000
VERSION     DASUCY010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1526209"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /gene="secF"
                     /locus_tag="VFV25_00035"
     CDS             <1..811
                     /gene="secF"
                     /locus_tag="VFV25_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016723719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEY1391730.1"
                     /translation="VQNFGTSHDVLIRLPLHGDSKQQAAVVDRVFGALCQAEGGTLQQ
                     TQGVSDKGEAVAKQACQAGAAEPVRLSRSEYVGPQVGSELALNGAYALLATVIGIVLY
                     LSFRFEWKFAIAGIIANLHDVVIILGFFAFFQWEFSLSVLAAVLAVLGYSVNESVVIF
                     DRVREAFRKFRKMNTHEVIDHAITSTMSRTIITHGSTQMMVLSMLAFGGPTLHYFAIA
                     LTIGILFGIYSSVFVAAALAMWLGVKREDLMKPGSGRKEGDQDDPNAGAVV"
     gene            853..>2220
                     /locus_tag="VFV25_00040"
     CDS             853..>2220
                     /locus_tag="VFV25_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HEY1391731.1"
                     /translation="MATPPAQTSALATQARRAYVDFLLQGVGPVVTNVTEGARSLANQ
                     AADPETSQKRRNLAMDLPKSCTRWQNAMVTALRSASFSLGSGGPVTLEVAGAGRTAAK
                     SGEFSLVDDDTIEREILSSRLALAMMDKASWEFTDLRSRISVLEQREEMDAHDVLRPH
                     VLASFVIDAWRAATLDLDAWRTLQPLLHDEFTHFVEEAYHECNRWLLDHGVRPEIDLR
                     PFIKRTGSALPSGPSSGPAPLGGASASAGLPGQAVGEETRLMTRTSGLPAHGSQHAEA
                     VLGRLNRLIGRQVPEFSSSTIMQPSPALKAAISEAQHTLTQHLSAPSARGRDRGTAPP
                     VISTPALLQELQQRKQALKQAASTPVERATIEIVALLFQSILTEERIPAAVRVWFARL
                     QMPVLRVAVSEPDFFATIDHPARKLIDRMGACVMGFDATTRAVGDALEKEIKRVVQVV
                     EAYPDT"
BASE COUNT          367 a          759 c          738 g          356 t
ORIGIN      
        1 ggtgcagaac ttcggcacct cgcacgacgt gctgatccgc ctgccgctgc acggcgacag
       61 caagcaacag gccgcggtcg tcgatcgcgt cttcggcgcg ctgtgccagg ccgagggcgg
      121 cacgctgcag cagacccagg gggtcagcga caagggcgag gcggtcgcca agcaggcttg
      181 ccaggccggc gccgccgagc cggtacggct gtcgcgcagc gaatacgtcg ggccgcaggt
      241 cggctccgag ctggcgctca acggcgccta tgcactgctg gcgaccgtga tcggcatcgt
      301 gctatatctg tccttccgct tcgaatggaa gttcgccatc gccggcatca tcgccaacct
      361 gcacgacgtt gtgatcatcc tcggcttctt cgccttcttt caatgggagt tctcgctctc
      421 ggtgctggcc gccgtgctgg cggtgctggg ctattcggtc aacgaatcgg tggtgatctt
      481 cgaccgggtg cgcgaggcct tccgcaagtt ccgcaagatg aacacgcatg aggtgatcga
      541 ccacgccatc acctcgacca tgagccgcac catcatcacc cacggcagca cgcagatgat
      601 ggtgctgtcg atgctggcct tcggcgggcc gacgctgcac tacttcgcga tcgcgctgac
      661 gatcggcatc ctgttcggca tctattcatc ggtgttcgtg gcggccgcgc tcgccatgtg
      721 gctgggtgtc aagcgtgaag atctgatgaa gcccggcagc ggccgcaagg aaggcgatca
      781 ggacgatccc aacgcggggg ccgtggtgta gagttcacgg aacttcacac tgtcgagtac
      841 ctagcgcccc tcatggcgac gcctcccgcc caaacttcgg ctctggccac ccaggcgcgg
      901 cgcgcctatg tggacttcct gctgcagggc gtgggaccgg tggtgacaaa cgtgacagag
      961 ggtgccagat cgctggccaa ccaggccgcc gaccccgaga ccagccagaa gcgccgcaac
     1021 ctggcgatgg acctgcccaa gtcctgcact cgctggcaga acgccatggt cacggcgctg
     1081 cgcagcgcca gcttcagcct gggcagcggc ggcccggtga ctctggaagt cgccggcgca
     1141 gggcggactg cagccaagag cggcgagttc tcgctggtcg acgacgacac catcgagcgc
     1201 gagatcctca gctcccgcct ggccctggcg atgatggaca aggcctcctg ggagttcacc
     1261 gacctgcgtt cgcgcatctc ggtgctcgag cagcgcgaag agatggacgc gcacgacgtg
     1321 ctgcgccccc atgtgctggc gagcttcgtg atcgatgcct ggcgcgccgc aacactggac
     1381 ctggacgcct ggcgcaccct gcagccgctg ctgcacgacg aattcacgca tttcgtcgaa
     1441 gaggcctatc acgagtgcaa ccgctggctg ctcgaccatg gcgtgcgccc cgagatcgac
     1501 ctgcgtccct tcatcaagcg cacgggcagc gccctgccga gcggtccttc gagtggcccc
     1561 gcgccgctgg gcggagcctc ggcgagcgcc ggtttgcccg gccaagccgt cggcgaagag
     1621 acgcgcttga tgacgcgcac ctccgggctg cccgcgcacg gctcccagca tgccgaggcg
     1681 gtgctggggc ggctgaaccg cttgatcggc cgccaggtgc cggagttcag ttcctccacc
     1741 atcatgcagc cctcgccggc actgaaggcc gccatcagcg aggcgcagca cacgctgacg
     1801 cagcatctgt cggcgccgtc cgcccgtggg cgcgaccggg gtaccgcgcc gccggtcatc
     1861 agcacccccg cgctgctgca ggaattgcag caacgcaagc aggccctcaa gcaagcagcg
     1921 tcaacgccgg tggagcgcgc gacgatagag atcgtcgcgc tgctgtttca gagcatcttg
     1981 accgaagagc gcattccggc cgccgtgcgc gtatggttcg cgcggctgca gatgccggtg
     2041 ctgcgcgtgg cggtgagcga gcccgacttc ttcgccacca tcgaccaccc ggcgcgcaag
     2101 ctgatcgacc gcatgggcgc ctgcgtgatg ggcttcgatg ccaccacgcg ggccgtgggc
     2161 gacgcgctgg agaaggaaat caagcgcgtg gtgcaggtgg tcgaggccta ccccgacacc
//
LOCUS       DASUCY010000003         7179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_803329, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000003 DASUCY010000000
VERSION     DASUCY010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 7179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7179
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803329"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1737)
                     /locus_tag="VFV25_00045"
     CDS             complement(<1..1737)
                     /locus_tag="VFV25_00045"
                     /inference="COORDINATES: protein motif:HMM:NF020036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEY1391732.1"
                     /translation="MDCSPSPALLAWSDASSGLAWEINAAARDWALARAWTQEHWNAL
                     AAAVLECTAPTRRSNAALGVRWRSVPEAGGLLAWLMPLEPGGVEAEVQALRERVAELS
                     GQLDAAQELGSRPGSLAVDSDSAELARRLQASSTQLALAVGLVGIVLWRYDFAAGLMQ
                     FNDQGYKLLGFKRRAEGVPLAEARGVIHPQDREQVELAAQQAVASIGVVDVEARYMTA
                     AGTWSTQLTRRIAERDPQGKAVALVGVSMDMTEQRATQERLRQAEQRAMIAIQAASMG
                     VWEFNLLTGEVYWDVQMYALRGLDPADSRPPALLRDLAVHPHDLPRLQQRIHDVAEEG
                     TIYQEQYRVVWPDGSVHWLAVRGMVARDARGRGTRMFGVNWDITEHQLNEDMRRDKAA
                     AEQASRAKSEFLAHMSHELRTPLNAVLGFAQLMLEDPGEPLERQQRLRAQRIRNAGKH
                     LLALIDDVLDFSSMESGSLPLDREPVALGPLVQETLQWLAPAAERAGVSLHAPSSDGE
                     VMGDERRLRQVLANLLSNAVKYNRPGGRVELALIDSAAADGMLGFSVRDTGRGLAPEQ
                     LRRLFEPFNRLGA"
     gene            complement(1821..2375)
                     /gene="msrA"
                     /locus_tag="VFV25_00050"
     CDS             complement(1821..2375)
                     /gene="msrA"
                     /locus_tag="VFV25_00050"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEY1391733.1"
                     /translation="MTSETQTETITLGGGCFWCLEAVYLDVEGVTSVESGYSNGQALD
                     PSYEDVCTGRTGHAEVVRVGFDPQRVSLRQLLEIFFVIHDPTTLNRQGNDVGTQYRSG
                     IYTHSEAQAAVAREVIAELAASGVYGAQPVVTEVQPESNYWPAEDYHQRYFERHPNQG
                     YCAFVVAPKLEKFRKTFATQQRRR"
     gene            complement(2451..2963)
                     /locus_tag="VFV25_00055"
     CDS             complement(2451..2963)
                     /locus_tag="VFV25_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY1391734.1"
                     /translation="MSEETKADQLRAKLIRNFDTADKQVEQRDPLYHTLCARLGTGTA
                     ASKLGASIDIVSPGKRSCPYHYHYAQEEMFVVLEGRGSLRVAGEMLPVEVGDVVFIPP
                     GPEYPHQFINTSDAPLKYLSISTREAPEICVYPDSDKYLAYAGGHEQGEGVDAIHRAK
                     DQLDYWDGEP"
     gene            complement(2965..5142)
                     /locus_tag="VFV25_00060"
     CDS             complement(2965..5142)
                     /locus_tag="VFV25_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY1391735.1"
                     /translation="MARLGALLLGCAGAVAQTLPVAPVRDVVDHFYGTEVHDPYRYFE
                     DIKSPEVAAWMKAHSDAAHATLARIAGRDALLRRLTEMENASAARVALPVRTTDGRLF
                     YEKRGASDNQYKLYVREQGGQERLLVDPEALARSTGKPHAVNYFMPSRDGRYLAYGIS
                     AGGSEAASLVVLDVRSGKAIGKPISRADFGLPDWSADGRYLSFMRLQALRPGAPPTDK
                     YKRGECFVVDLHSGRLVDKPVFGYRARGTKIADDENPFVELTADGRWTLGVVANGVQR
                     ELRLYVAPQAAALRGTASWREVATTADAITAIAYHGNTLYALSHKDASRFKLLQFKLD
                     AADAGWREILPVGERVLTDIAAASDALYIEERDGNIKRLFKRAYADEAKVQEVALPLQ
                     GAFSLSGFESNYSAASPRLPGVLLSLESWTRAGQIYQVAADGSVTNTGLQPAGPFDAP
                     SDVEATEVLVKSYDGALVPLSIVHRKDVKLDGNNPTILYGYASYGITEEPFFSTGVMA
                     WLQAGGVYAFANPRGSGVYGEDWYKAGKQANKPNTWKDFIACAEYLIAQRYTSPLRLG
                     IYGGSAGGILVGRTMTERPDLFAAAVSAVGLSDAVRAEIGPNGPPNVPEFGSVKTEAG
                     FRALLEMSTYHHIVDGVKYPAVMFTHGINDPRVEVWQSTKTAARLMAATASGKPVLLR
                     LDYQAGHGIGNTKEQRLTERADVYAFFLWRMGVPGFELKDSGG"
     gene            5351..6151
                     /locus_tag="VFV25_00065"
     CDS             5351..6151
                     /locus_tag="VFV25_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEY1391736.1"
                     /translation="MFGLEPWKPILSALALPPVPLLLLILLGARLIPLRRWLGFSMVF
                     VSVTLLWLSACQGTGRWLQSVALRPPQVLSSSELARLQAEGKSGKPDIAIVVLGGGLV
                     SRAPEYGVSDLTRLSLQRLRYGVYLSRATGLPLAYSGGVGWADGDAATSSEAEVAARI
                     TQQEYRLPLRWVESASRDTRENAGRTVPLLRAAGIKEIVLVTHAWHMPRALRVFEEAA
                     HGELRITPAPMGYFTQPSWRALDWLPTREGMEQVALALHELLGLAVHA"
     gene            complement(6388..>7179)
                     /locus_tag="VFV25_00070"
     CDS             complement(6388..>7179)
                     /locus_tag="VFV25_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEY1391737.1"
                     /translation="EAGLKLQVPASAEIVLEGHIPVAAAGWRGVSEKGVQLKEQGGYL
                     YALEGPFGDHTGYYNEQEWFPVFQIDRMTHRRDPIYHSTYTGKPPDEPAVLGVALNEV
                     FVPILQKQFPEIVDFYLPPEGCSYRMAVISMRKSYPGHAKRLMFGLWSFLRQFMYTKF
                     IVVTDDDIDIRDWKDVIWAITTRVDPVRDTTLVDQTPIDYLDFASPTSGLGGKMGLDA
                     TNKWPGETAREWGRAITMGVEVEQRVAGLFEAIMNGGKVSSAADQ"
BASE COUNT         1073 a         2437 c         2410 g         1259 t
ORIGIN      
        1 ggcgccgagc cggttgaagg gttcgaacag ccggcgcagc tgctcgggcg caaggccacg
       61 cccggtgtcg cgcacgctga agcccagcat gccgtcggct gctgctgagt cgatcagcgc
      121 cagttcgaca cggccgcccg gacggttgta cttgacggcg ttggacagca gattggccag
      181 cacctggcgc agccgccttt cgtctcccat cacctcgcca tccgacgacg gcgcatgcag
      241 gctcacgccg gcgcgctcgg cggccggtgc cagccattgc agcgtctcct gcaccagcgg
      301 ccccagcgcc acgggctccc tgtccagcgg caggctgcca ctttccatgc tgctgaagtc
      361 cagcacgtcg tcgatcaagg ccagcaggtg cttgccggca ttgcgtatgc gctgggcgcg
      421 caggcgctgc tgccgttcca gcggctcgcc gggatcttcg agcatcagct gcgcgaagcc
      481 cagcaccgcg ttgagcggcg tgcgcagctc gtggctcatg tgcgcgagga actcgctctt
      541 agcccggctg gcctgctcgg cggcggcctt gtcgcggcgc atgtcttcgt tgagctggtg
      601 ttcggtgatg tcccagttga cgccgaacat gcgcgtcccc cgcccgcggg catcgcgcgc
      661 gaccatgccg cgcacggcca gccagtgcac cgagccgtcc ggccatacga cgcgatactg
      721 ctcctggtag atcgtccctt cctcggcgac atcgtggatg cgctgctgca gccggggcag
      781 atcatgggga tgcactgcca ggtcgcgcag cagggctggc ggccgggagt cggccggatc
      841 caggccgcgc agcgcataca tctgcacatc ccagtagacc tcgccggtga gcaggttgaa
      901 ctcccagacc cccatgctgg cagcctggat ggcgatcatg gcgcgctgct cggcctggcg
      961 cagtctttcc tgcgtggcgc gctgctcggt catgtccatc gacacgccga ccagggccac
     1021 ggccttgccc tgcggatccc gctcggcaat gcggcgcgtc agctgggtgc tccaggttcc
     1081 ggctgccgtc atgtagcggg cctccacatc gaccacgccg atgctggcca ccgcctgctg
     1141 cgcggccagc tcgacctgtt cgcgatcttg cgggtggatg acgccgcgcg cttcggccag
     1201 cggcacgcct tcggcgcgac gcttgaagcc cagcagcttg tagccctggt cgttgaattg
     1261 catcagcccg gcggcaaagt cgtatcgcca cagcacgatg cccaccaggc cgacggccag
     1321 ggccagctga gtgctcgagg cctgcaagcg tcgggccagc tccgcactgt cggagtcgac
     1381 tgccagcgag cctgggcgac tgccgagttc ctgcgccgcg tccaattgcc cgctcagctc
     1441 ggcgacgcgc tcgcgcagtg cctgcacttc ggcttcgaca ccgcccggct ccagcggcat
     1501 caaccatgca agcaggccgc ccgcctcggg cacgctgcgc cagcgcaccc ccagcgccgc
     1561 gttgctgcgc cgggtcgggg ccgtgcactc cagcaccgcc gcggccaagg cattccagtg
     1621 ctcctgggtc caggcgcgag ccagcgccca atcgcgcgct gccgcgttga tctcccaggc
     1681 caggccggag gaggcatcgc tccaggccag cagggccgga ctgggcgagc agtccacggc
     1741 ggcggggagg tgcggttgct gcatgcagtc tgaggctgtg atcccgcccc agtgtagcga
     1801 gcgggtgcag ggatccagct tcagcgacgc cgctgttgtg tggcaaaagt cttgcgaaac
     1861 ttctccagct tgggtgcgac gacgaaggcg cagtagccct gattgggatg gcgctcgaaa
     1921 tagcgctggt ggtagtcctc ggctggccag tagttggact cgggctgcac ttcggtcacg
     1981 acgggctggg cgccgtagac gcccgaggca gccagctcgg cgatcacctc ccgcgcgacg
     2041 gctgcctgcg cctcgctgtg cgtgtagatg cccgaacggt attgcgtgcc gacatcattg
     2101 ccctggcggt tgagcgtggt cgggtcgtgg atcacgaaga agatctcgag cagctggcgc
     2161 agggaaacgc gctgcgggtc gaagcccacg cgtaccacct ccgcgtggcc ggtgcgaccg
     2221 gtgcagacgt cctcgtagct gggatccagc gcctggccgt tgctgtagcc cgactcgacc
     2281 gaagtcacgc cttcgacgtc aaggtagacg gcctcgaggc accagaagca gccgccgccg
     2341 agggtgatgg tttctgtctg cgtctcggat gtcatcgctt gctccatgtt ggcctgatgg
     2401 tggatctgtc gccaccggcc atgcacacct tacggggcaa atgccgggtc tcagggctcc
     2461 ccgtcccagt agtccagctg gtccttggcg cggtggatgg catcgacgcc ttcgccttgc
     2521 tcatggcccc cggcatacgc caggtacttg tcggagtccg ggtagacgca gatctcgggc
     2581 gcctcgcggg tgctgatcga caggtacttc agaggcgcat cggacgtgtt gatgaactgg
     2641 tgcggatact ccggccccgg cgggatgaac acgacgtcgc cgacttcgac cggcagcatc
     2701 tcgccggcca cccgcaggct gccccggccc tccagcacca cgaacatttc ctcctgcgcg
     2761 tagtgatagt ggtaagggca actgcgcttg cccggcgaca cgatgtcgat gctggcgccc
     2821 agcttgctgg ccgccgtgcc ggtaccgagc cgggcgcaca gcgtgtggta cagcggatcg
     2881 cgctgctcca cttgcttgtc cgcggtgtcg aagttgcgga tcagcttggc acgcagttgg
     2941 tctgctttcg tctcctcgct catgtcaacc tcccgagtct ttgagctcaa agcccggcac
     3001 acccatccgc cacagaaaga aggcataaac gtcggcgcgc tcggtgagcc gctgttcctt
     3061 ggtgttgccg atgccgtggc cggcctggta gtccaggcgc agcagcacgg gcttgccgct
     3121 cgcggtggcg gccatcagcc gcgccgccgt cttggtgctt tgccagacct cgacgcgcgg
     3181 atcgttgatg ccgtgcgtga acatcacggc cgggtatttc acgccgtcga cgatgtggtg
     3241 gtaggtgctc atctccagca gcgcacggaa gcccgcctcg gtcttcacgc tgccgaactc
     3301 ggggacgttc ggcgggccgt tggggccgat ctcggcccgc accgcgtccg acaggcccac
     3361 cgccgaaacg gcggcagcaa acagatcggg ccgctcggtc atggtccggc cgaccaggat
     3421 gccgcccgcg ctgccgccgt agatgcccag gcgcagcggc gaggtgtagc gttgggcgat
     3481 caggtattcg gcgcaggcga tgaagtcctt ccaggtattg ggcttgttcg cctgcttgcc
     3541 ggccttgtac cagtcctcgc cgtagacgcc gctgccgcgc ggattggcga aggcatagac
     3601 gccgcccgcc tgcagccagg ccatcacgcc ggtgctgaag aagggctctt cggtgatgcc
     3661 gtaggacgcg tagccgtaga ggatggtcgg gttgttgccg tccagcttca cgtccttgcg
     3721 atgcacgatg gacagcggca ccaaggcgcc gtcgtagctc ttgaccagca cctcggtggc
     3781 ctccacgtcg ctcggcgcgt caaaggggcc ggccggctgc aggccggtgt tggtaacgct
     3841 gccatcggct gccacctgat aaatctggcc ggcgcgggtc cagctttcca gggacagcag
     3901 cacgccgggc agccgggggc tggcggcgga atagttcgac tcgaagcccg acagcgagaa
     3961 ggcgccctgc agcggcagcg cgacttcctg caccttggct tcgtcggcat aggcgcgctt
     4021 gaacagccgc ttaatgttgc cgtcgcgctc ctcgatgtag agcgcgtcgg aggccgcggc
     4081 tatgtcagtg agcacgcgct cgcccaccgg caggatttcg cgccagccgg cgtcggccgc
     4141 atccagcttg aactgcagca gcttgaagcg cgaggcgtcc ttgtgcgaca gcgcgtacag
     4201 cgtattgccg tggtaggcga tggccgtgat cgcgtcggcg gtcgtcgcca cttctcgcca
     4261 gctggcggtg ccgcgcaagg ctgcggcctg cggtgccaca tagaggcgca gttcgcgctg
     4321 cacgccgttg gcgacgacac ccagcgtcca gcggccatcg gcagtgagtt cgacgaaagg
     4381 gttctcgtcg tcggcgatct tggtgccgcg cgcgcggtag ccgaacaccg gcttgtcgac
     4441 cagacgcccg ctgtgcaggt ccactacaaa gcattcgccg cgcttgtact tgtcggtcgg
     4501 cggggccccg gggcgcaggg cctgcagccg catgaagctc aggtagcgtc cgtcggccga
     4561 ccagtcgggc aggccgaaat cggcgcgcga gatcggcttg ccgatcgcct tgccgctgcg
     4621 cacgtccagc accacaagcg aggccgcctc ggagcccccc gccgagatgc cgtaggccaa
     4681 gtagcgcccg tcgcgcgagg gcatgaagta gttgaccgca tgcggcttgc cggtgctgcg
     4741 cgccagggct tcgggatcaa ccagcaggcg ctcctggccg ccctgttcgc gcacatagag
     4801 cttgtattgg ttgtcgctgg cgccgcgctt ctcgtagaac aggcggccgt cggtggtgcg
     4861 caccggcaac gcgacgcgcg ccgcgctggc gttttccatc tcggtcagcc gccgcagcag
     4921 ggcatcgcga ccggcgatgc gcgctagcgt cgcatgggcc gcgtcgctgt gtgccttcat
     4981 ccatgcggcg acctcgggcg acttgatgtc ctcgaagtag cgataggggt cgtgcacctc
     5041 ggtgccgtaa aagtggtcca cgacgtcgcg caccggcgca acgggcaggg tctgcgccac
     5101 cgcgccggcg cagccgagca acagcgcgcc cagccgcgcc ataagaacgc cccatctcgc
     5161 ctgcctacca tgcatggaaa actccccggc gtgattgatg aaagggccgc agcttggcat
     5221 gagcttcgct cgggcacaat cccgcgcctt gcactgccct ctcaaggaat ggatgctgcc
     5281 ccgtttcctt gacccttggg gcgggctcga cttacccagc ccttggcggg tgatcaagga
     5341 gacagtcctc atgttcggcc tcgagccctg gaagcccatc ctcagtgccc tggcgttgcc
     5401 gccggtgccg ctgctgctac tgattctgtt gggtgcccgc ttgattcctc tgcggcgctg
     5461 gctgggtttt tctatggtct ttgtgagcgt cacgctgctt tggctgagcg cctgccaagg
     5521 caccgggcgc tggctgcaga gcgtcgcgct caggccgccg caggtcttga gcagcagcga
     5581 gttggcccgc ctgcaggccg aaggcaagtc cgggaagccg gacatcgcga tcgtcgtgct
     5641 gggcggcggg ctggtatccc gggcgccgga atacggcgtc tcggatctca cgcgcctgtc
     5701 gctgcaacgc ctgcgctatg gcgtgtatct cagccgagca actggcttgc cccttgccta
     5761 cagcggcggc gtaggctggg ccgacggcga cgcggcgaca agctcggagg ccgaagtcgc
     5821 cgcccgcatc acccagcagg aatacaggct gccgttgcgc tgggtcgagt cggcctcgcg
     5881 cgacacccgc gagaacgccg gccgcaccgt gccgctgctg cgcgccgccg gcatcaagga
     5941 gatcgtgctg gtgacccatg cctggcacat gccgcgcgcg ctgcgggtct tcgaggaggc
     6001 cgcccacggc gagctgcgca tcacgccggc gcccatgggc tacttcacgc agccttcctg
     6061 gcgtgcgctg gactggttgc ctacgcgcga gggcatggag caggttgccc tggcgctgca
     6121 cgagctgctg ggcctggcgg tgcatgcctg aagcgccggc ctgcgcagaa tcggaccccc
     6181 aaaatgaaac ccctcccggc cttgggcggg aggggtgagt ggctgacgcc actgttccgg
     6241 ggctccggag gaagaatggt ccggggctcc tggcgtgcag agactgcacc tttgagaatg
     6301 cctgcggaca ggcgtattta gattaggaga agcgagccgc cgcttcaagc ccggcatcgc
     6361 gcgactgttc tatgacggct tggctcctca ttgatccgcc gcgcttgaca ccttgccgcc
     6421 attcatgatt gcctcgaaga ggccggcgac gcgctgctcc acctcgacgc ccatcgtgat
     6481 ggcccggccc cattcgcgcg ccgtctcgcc gggccacttg ttggtggcgt ccaggcccat
     6541 cttgccgccc aggccgctcg tcggcgaagc gaaatccagg tagtcgatcg gcgtctggtc
     6601 gaccagcgtg gtgtcgcgca ccgggtcgac ccgggtcgtg atggcccaga tcacatcctt
     6661 ccagtcgcgg atgtcgatgt catcgtcggt cacgacgatg aacttggtgt acatgaattg
     6721 gcgcagaaag ctccacagcc cgaacatcag ccgcttcgca tggccggggt agctcttgcg
     6781 catgctgatc accgccatgc ggtagctgca gccctcgggc ggcaggtaga agtccacgat
     6841 ctcgggaaac tgcttctgca agatcggcac gaagacttcg ttcagcgcca cgcccagcac
     6901 tgccggttcg tcgggcggct tgccggtgta ggtggagtga tagatcgggt cgcgccggtg
     6961 ggtcatgcga tcgatctgga acaccgggaa ccattcctgc tcgttgtaat agccggtgtg
     7021 gtcgccgaag ggtccctcca gcgcatatag atagccgccc tgctccttca actgcacgcc
     7081 cttctcgctg acgccgcgcc agccggccgc cgcgaccggg atatgccctt ccagcacgat
     7141 ctctgcgctg gccggcacct gcagcttgag ccccgcctc
//
LOCUS       DASUCY010000004         2614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_610597, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000004 DASUCY010000000
VERSION     DASUCY010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610597"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /gene="xseA"
                     /locus_tag="VFV25_00075"
     CDS             complement(<1..1158)
                     /gene="xseA"
                     /locus_tag="VFV25_00075"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEY1391738.1"
                     /translation="MSAPPNFVAPARQVWGVAALVHAIGDVLAARFAACTVRGELAAF
                     TRAASGHCYFSLQDEEGTALLRCAMFRRAASLLDFEPGEGLLVEVRGRLAVYEPRGEL
                     QFVVESMRRAGAGALYEQFLRLKAKLEAQGLFDAERKRDIAVFPRRVGVVTSLAAAAL
                     HDVLTAFARRSPQVEVIVYPSAVQGPEAPQQLVQAITEAGRRNEVETLIVCRGGGSLE
                     DLWAFNDERVVRAIASCPVPVISGVGHETDVTLADLAADLRAPTPTAAAELAAPLRDE
                     ALGVLQAWADRARTSAYRRLDMEAQRLDRDSLRLARPAQLLQRRRQALELLQQGWRLA
                     ISRALALRRQHQVYLAQRLQRAGALTTLLPRQRLELLATRLQALDPTQVLRR"
     gene            complement(1258..1521)
                     /locus_tag="VFV25_00080"
     CDS             complement(1258..1521)
                     /locus_tag="VFV25_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1391739.1"
                     /translation="MIDSKKSPRTSSLRRAIMTLRDCRSMARGLRATPVLQPSTVAVD
                     NFVGNPYRRRREARKIKARNKMPKQKAVENPFASSTCANVKAL"
     gene            1508..2158
                     /locus_tag="VFV25_00085"
     CDS             1508..2158
                     /locus_tag="VFV25_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEY1391740.1"
                     /translation="MLSIIQAAGWPIWPLLLCSFVALALVIERVTSLRPARVAPPKLL
                     DEVISYTRANLPSAEVVNKLAASSVLGTVLASGLRALIGEPRITEQALRGSFESAGRA
                     AIHRLERYLNTLGTIAAAAPLMGLLGTVVGMIEIFGSQSPTTAAAPEQLAHGISVALY
                     NTAFGLIVAIPSLMFYRYFRGLVDEYTIELEQAAERMVPHLMRFSRQASAAAPSKP"
     gene            2155..2583
                     /locus_tag="VFV25_00090"
     CDS             2155..2583
                     /locus_tag="VFV25_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY1391741.1"
                     /translation="MNFQHRRSEEPEINLIPFIDVLLVVLIFLMLSTTYSKYTELKIM
                     LPVADAERSRDYPQQIIVSVAHDGRYAVNKKPVEGRSVELLAAELAAAAGNRKDVVVI
                     VSADAMTAHQSVINVMDAARRVGLQQLTFATQSSHSGAGN"
BASE COUNT          436 a          920 c          848 g          410 t
ORIGIN      
        1 gcgacgcagc acctgcgtcg gatccagggc ctgcagccgc gtcgccagca gctccagccg
       61 ttgccgcggc agcagcgtgg tcaatgcgcc ggcgcgctgc aaccgctgcg cgagatagac
      121 ctggtgctgg cgccgcaagg ccagcgcacg cgagatcgcc agccgccagc cctgctgcag
      181 cagctccagc gcctggcggc ggcgctgcag caactgggcg ggccgcgcca gcctcagact
      241 gtcgcggtcc aggcgctggg cctccatgtc gaggcggcga tacgcgctgg tacgtgcgcg
      301 atcggcccag gcctgcagca cgccgagggc ttcgtcgcgc agcggcgcag ccagctcggc
      361 cgcagcggtc ggcgtgggcg cgcgcaaatc ggccgcgaga tcggccagcg taacgtcggt
      421 ctcatggccg acgccgctga tcacagggac ggggcaggac gcgatggcgc gcaccacgcg
      481 ctcgtcgttg aatgcccaca ggtcttccag cgagccgccg ccgcggcaga cgatcaaggt
      541 ctcgacctcg ttgcgtcgcc ctgcttctgt gatcgcctgc acgagctgct ggggtgcctc
      601 ggggccctgc acggcgctgg gatagacgat cacctcgacc tgcggcgagc gccgcgcgaa
      661 ggcagtcagt acatcgtgta gggccgcagc tgcgagcgag gtcacgacgc cgacacggcg
      721 cggaaaaacg gcaatgtcgc gcttgcgctc ggcatcgaac aagccctgcg cctccagctt
      781 ggccttgagc cgcaggaact gctcgtagag cgcaccggca ccggcgcgcc gcatgctctc
      841 gacgacgaac tgcagctcgc cgcgcggctc gtagacggcc agccgcccgc gcacctccac
      901 cagcagccct tcacccggct cgaagtcgag caggctggcg gcgcggcgga acatcgcgca
      961 acgcagcagc gccgtgcctt cctcgtcctg caggctgaaa tagcaatggc cgctggccgc
     1021 ccgcgtgaac gcggccagct cgccacgcac cgtacaggca gcgaagcggg cggccagcac
     1081 atcgccgatg gcatgcacca gcgcggccac gccccacacc tggcgcgccg gcgcaacaaa
     1141 gttcggcgga gcactcacca cttgtgcgac gcagcgcaaa cccgcgcaag ccaatgccgg
     1201 cgcggctcgg gaactccaca agcgcatggc gcttgggccg gcggggctgg ttccatgtca
     1261 caaggcttta acattcgcgc aagtgcttga tgcaaaagga ttttctactg ccttttgttt
     1321 aggcattttg ttccgggcct tgatttttcg ggcttcgcga cggcgccggt aagggttgcc
     1381 cacaaagttg tccacagcaa cggtggatgg ttggagcact ggtgtggcac gcaagccgcg
     1441 cgccatcgaa cggcagtccc gcagcgtcat aatcgcgcgt ctcaaagacg acgttcgagg
     1501 ggatttcttg ctgtcgatca tacaagccgc cggctggccg atctggccgc ttctactgtg
     1561 ttctttcgtg gccctggccc tcgtcatcga gcgcgtgacg agcctgcgcc ctgcccgcgt
     1621 cgcgccgccc aagctgctgg acgaggtgat ttcctacacg cgcgccaatc tgccctcggc
     1681 tgaagtcgtg aacaagctgg cggccagctc ggtgctcggc acagtgctcg cctcggggct
     1741 gcgggccttg atcggcgagc cgcgcatcac cgagcaggcc ttgcgcggca gcttcgaatc
     1801 ggctgggcgc gcagcgatac acaggctgga acgctacctc aacacgctgg gcacgatcgc
     1861 cgccgcggcg ccactgatgg gcctgctggg cacggtggtc ggcatgatcg agatcttcgg
     1921 ctcgcagtcg ccgaccaccg cggccgcccc cgaacagctc gcgcacggca tctcggtggc
     1981 cttgtacaac accgccttcg gcctcatcgt cgcgattccc tcgctgatgt tctatcgcta
     2041 cttccgcggc ctcgtcgacg agtacacgat agaactggag caggcggccg agcgcatggt
     2101 gccccacctg atgcgcttct cgcgccaagc cagcgccgcg gcaccgagca agccatgaac
     2161 ttccaacatc gtcgcagcga ggaaccggag atcaacctga tccccttcat cgacgtgctg
     2221 ctcgtggtgc tgatcttcct gatgctgtcg accacctact cgaaatacac cgagctcaag
     2281 atcatgctgc cggtggcgga tgccgagcgc tcgcgcgact acccgcagca gatcatcgtc
     2341 tcggtcgccc acgacgggcg ctatgcggtc aacaagaagc cggtcgaagg gcgcagcgtt
     2401 gagctgcttg ccgccgagct ggccgccgcg gcaggcaatc gcaaggacgt ggtcgtcatc
     2461 gtctcggccg acgccatgac ggcgcaccag agcgtcatca atgtgatgga cgcggcacgc
     2521 cgcgtgggcc tgcagcagct caccttcgcg acccaatcct cgcattccgg cgcgggcaac
     2581 tagggcgccc cgccttgacc gcctcgagct ggtc
//
LOCUS       DASUCY010000005         2126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_996174, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000005 DASUCY010000000
VERSION     DASUCY010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996174"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1162
                     /gene="lplT"
                     /locus_tag="VFV25_00095"
     CDS             <1..1162
                     /gene="lplT"
                     /locus_tag="VFV25_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HEY1391742.1"
                     /translation="SAHWQVPLLKLCFTIAYVVLAPFAGPLADAWPKGRVMLATNSIK
                     AAGCLLIAAGCDPLLGFVIVGLGAAGYSPAKYGLITEIVPPQRLVEANGWIEVSTVGA
                     IILGTVLGGALVGPLADAAWLLWTPPAAMQATTRLLLPLLLVLALYALAAALNFGLPD
                     SGARYAAASRRPLALLAEFRRDNAALWRDPEARISLAVTTLFWGVGATLQFLVLRWAI
                     EALDLDLHQAAYLQGVTAIGITAGAALAGRCVPLGRAGTVLPLGIAMGLLVPLMTATG
                     SVATALPLLILVGGCAGFFVVPMNALLQHRGYVLLSAGRSIAVQNFNENLSVLLMLAA
                     YAALLAFGVPIAWLVWGFGIAVAVVMALIGSAYHKSRRSGAASLPAYPQEHA"
     gene            1159..>2126
                     /locus_tag="VFV25_00100"
     CDS             1159..>2126
                     /locus_tag="VFV25_00100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEY1391743.1"
                     /translation="MKILIVTDAWEPQVNGVVRTLKMTVRELRARGCEVEIISPQGFR
                     SMPCPTYPEIRLALTTRRELERRIDAIAPQCVHIATEGPLGWAVRGICKKRGWQFTTA
                     YHSRFPEYLHARFRLPVSLSYSVLRRFHNAGAATLAPTPAIVDDLRSRGFTQAQIWSR
                     GVDLAMFGVEGEKLPRPSEPIFLYVGRLAVEKQVDAFLALDLPGHKWVAGEGPERARL
                     ETKYPGVRWFGVLDGPALAKLYRTADVMVFPSLTDTFGLVMVEAMACGTPVAAFPVPG
                     PVDVVGQSPGGVLHEDLRVACLKALELPREGVRRHAEQFSWAAATE"
BASE COUNT          271 a          767 c          730 g          358 t
ORIGIN      
        1 cagcgcccac tggcaggtgc cgctgctcaa gctgtgcttc acgatcgcct atgtggtgct
       61 ggcgcccttc gccgggccgc tggccgatgc ttggcccaag ggccgggtga tgctggcgac
      121 caacagcatc aaggcggccg gctgcctgct gatcgcggct ggctgcgatc cgctgctggg
      181 cttcgtcatc gtgggcctcg gcgcggccgg ctattcgccg gccaagtacg gcctcatcac
      241 cgagatcgtg ccgccgcagc gcctcgtgga ggccaacggc tggatcgagg tgagcacggt
      301 gggcgcgatc attctcggga ccgtgctggg cggcgcactg gtggggccgc tcgccgatgc
      361 ggcctggctg ctgtggaccc caccggccgc gatgcaggcc acaacgcggc tgctgctgcc
      421 tctgctgttg gtgctcgccc tgtatgcact ggccgcggcc ctgaacttcg gcctgccgga
      481 cagcggcgcg cgctatgccg cagccagccg ccgcccgctc gcgctgctgg ctgagttccg
      541 gcgcgacaac gctgcgttgt ggcgcgaccc ggaagcccgc atctcgctcg cggtgaccac
      601 gctgttctgg ggcgtgggcg ccacgctgca gttcctggtg ctgcgctggg ccatcgaggc
      661 cctggacctc gatttgcacc aggccgccta cctgcaaggc gtcacggcca tcggcatcac
      721 ggccggcgcc gcgctggcgg gccgctgcgt gccgctcggg cgcgccggca cggtgctgcc
      781 gctgggcatt gcgatgggcc tgctggtgcc gctgatgacg gccaccggca gcgtcgccac
      841 ggccctgccc cttctgatct tggtgggcgg ctgcgccggc ttcttcgtcg tgccgatgaa
      901 tgcgctgctg cagcaccgcg gctacgtcct gctcagcgcc ggccgctcga tcgcggtgca
      961 gaacttcaac gagaacctga gcgtgctgct gatgctcgcg gcctatgccg ccctgctcgc
     1021 cttcggcgtt ccgatcgcct ggctggtgtg gggcttcggc atcgccgtcg ccgttgtcat
     1081 ggcgctcatc ggttcggcct atcacaaatc gcggcgcagc ggcgccgcct cgcttcccgc
     1141 ttacccacag gagcatgcat gaagatcctc atcgtcaccg acgcctggga accgcaagtc
     1201 aacggcgtgg tgcgcacctt gaagatgacc gtgcgcgagc tgcgcgcgcg cggctgcgag
     1261 gtcgagatca tctcgcccca gggctttcgc tccatgccct gccccaccta tccagaaatc
     1321 cggctcgcgc tcacgacgcg ccgcgagctg gagcgccgca tcgatgcgat cgcgccgcaa
     1381 tgcgtgcaca tcgccaccga aggcccgctg ggctgggccg tgcgcggcat ctgcaagaag
     1441 cgcggctggc agttcacgac ggcttatcac agccgcttcc ccgaatacct gcatgcgcgc
     1501 ttcaggctgc cggtgagctt gagctacagc gtgctgcgcc gcttccacaa tgccggcgcc
     1561 gccacgctcg cgcccacgcc ggccatcgtg gacgacctgc gctcgcgcgg cttcacgcag
     1621 gcccagatct ggtcgcgcgg cgtcgatctg gccatgttcg gcgtcgaagg cgagaagctg
     1681 ccgcgaccat cggagccgat cttcctctac gtcggccggc tcgcggtcga gaagcaggtc
     1741 gacgccttcc tggcgctgga cttgccgggc cacaaatggg tggccggcga aggcccggaa
     1801 cgtgcgcgcc tggaaaccaa gtaccccggc gtgcgctggt tcggcgtgct cgacggcccc
     1861 gcgctcgcga agctgtaccg aacggccgac gtgatggtct tccccagcct caccgacacc
     1921 ttcggcctcg tgatggtcga agcgatggcc tgcggcacgc cggtggccgc cttcccggtg
     1981 ccgggcccgg tggacgtggt cggccagtcg cccggcggcg tgctgcacga agacctgcgc
     2041 gtggcctgcc tgaaggcgct ggaactgccc cgcgaaggcg tgcggcgcca tgccgagcag
     2101 ttctcctggg ccgcggccac cgagca
//
LOCUS       DASUCY010000006         3368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_257174, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000006 DASUCY010000000
VERSION     DASUCY010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257174"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="VFV25_00105"
     CDS             complement(<1..674)
                     /locus_tag="VFV25_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1391744.1"
                     /translation="MGKKLAWAVISAAAVLAACGGGGGYGGGNSQPTSYSGLFVDAAA
                     GGVGYACGSSSGVTDQKGTFQYDAGSTCTFKIGGVALGSAAGAAVITPVTLVPGATNE
                     TNPVVINITRFLLSLDSGGNTDSMITIAPSVATALASSTLDFTNATTFDAQAQALVSM
                     AYPGRTLVAASVAQAHMGATLLGLLAGSYSCTYTGTSDSGMVTVTIANGAITGTGMSS
                     KSGSTQ"
     gene            complement(968..2014)
                     /locus_tag="VFV25_00110"
     CDS             complement(968..2014)
                     /locus_tag="VFV25_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEY1391745.1"
                     /translation="MDRTTYAVDIAKNVMQLHWVDAQTGEIGRKKLGRAKLAEYFARL
                     QPVRVAMEACGSAHHWGRLLGSLGHQVELLPARQVHAFVRGNKDDAADARAIWIAAQQ
                     TDIRRVPVKSEDQQAVLTLHRSRAHWVSVSTATINALRGLLYEFGVVLPRGSRVGLKA
                     LRAQRASIDAQLPQPMQRLLDVQLQALHDIDQRIEQIEAQIAAQHKQLKHAARLDRVP
                     GIGLLGATALAATLGDGSGYRSGREFSACLGLAPAHEGTGGKTRVGHLSKRGDPYLRT
                     LLIHGARAVVTGKHKPQWIERLLARRPLNVAVAALANKMARTAWALVAHGREWQRDWQ
                     SAKPGCGTAQAAAA"
     gene            complement(2249..2860)
                     /locus_tag="VFV25_00115"
     CDS             complement(2249..2860)
                     /locus_tag="VFV25_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY1391746.1"
                     /translation="METKTERSGLTLAAIVDTALQMAAADGLESLSIGEVAKRLGLSK
                     SGVFSRVGSREALQKAVSEEYDRRFLQDVFVPAMREPRGLPRLNAIAELWLKRCREVE
                     MHSGCIYCAGAFEFDDREGPLRDALLEGIRGWRTALKRTVIQAIDAGHFRPDTDAEQL
                     VFEIDGLFVALMREARFLREPRAAERAWACYERLIAARQAAAC"
     gene            complement(3001..>3368)
                     /locus_tag="VFV25_00120"
     CDS             complement(3001..>3368)
                     /locus_tag="VFV25_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY1391747.1"
                     /translation="AEKYPHELSGGQRQRVAIARAIAVEPEVIVLDEAVSALDVSVQA
                     QVLNLLRDLQDELGLAYIFISHDLAVVRFMADEVLVMKDGEVVERNATQALLDAPQQE
                     YTRRLLAAIPRGVEARIAA"
BASE COUNT          512 a         1161 c         1078 g          617 t
ORIGIN      
        1 aactgcgtcg agccggactt ggaggacatg ccggtgccgg tgatggcgcc gttggcgatc
       61 gtgacggtga ccatgcccga gtcgctggtg ccggtatagg tgcacgagta gctgccggcc
      121 agcaggccaa gcagggtcgc ccccatgtgg gcttgggcca cgcttgccgc caccagcgtg
      181 cggcctgggt aagccatgct gacgagggcc tgggcctgcg cgtcgaaggt cgttgcgttc
      241 gtgaagtcca gcgtcgacga ggcgagtgcg gtggcgacgc tgggggcaat ggtgatcatc
      301 gagtccgtgt tgccgcccga gtctagagat agcaggaagc gcgtgatgtt gatgacgacg
      361 ggattggtct cgttcgtcgc cccaggcacc agcgtcactg gcgtgatcac ggctgcgccg
      421 gccgccgaac ccagcgcgac gccgccgatc ttgaaggtgc aggtgctgcc cgcgtcgtat
      481 tggaaagtgc ctttctggtc ggtgacgccg ctcgaggagc cgcaggcgta gccgacgccg
      541 cccgccgcgg cgtcgacgaa gaggcccgaa taggacgtcg gctgcgaatt gccgccgccg
      601 tagccgccgc ctccgccgca ggccgccaag accgccgctg ccgaaatcac agcccatgcc
      661 agcttcttgc ccatgactgt cttatatgga cgcctcccgt gtggcaagcg agttcttgaa
      721 gttctgactg ggcggaacga ctgcggtctt atatccggcc ttgctgtgca gtccgggcgt
      781 aagcccgctg ctggccttga tggtcagtcg ctgggtcggg gctgatctcc tacagcgggc
      841 ttcgcgttgg cttgccctga cgatcattca gatttgcccg gcccgcgggt tggaccgctt
      901 tgccatcact tcatcgactc gactcgcgca ctctttgcgg catccactcc tgtagctttc
      961 agttgatcta cgcggcggct gcttgcgcag tgccgcaccc gggcttggcg ctttgccaat
     1021 ccctttgcca ctcacgccca tgagccacca gcgcccaggc cgtgcgtgcc atcttgttgg
     1081 ccagcgccgc caccgccacg ttcagcggtc ggcgcgccag cagccgttcg atccattgcg
     1141 gcttgtgctt gcctgtgacc accgcccgtg cgccgtggat cagcagcgtg cgcaggtacg
     1201 ggtcgccgcg cttgctcagg tggcccaccc gcgtcttgcc gcctgtgccc tcgtgcgctg
     1261 gcgccaggcc caggcaggcc gagaactcgc gcccgctgcg atagccgctg ccgtcgccca
     1321 gcgtggcggc caaggcggtg gcaccgagca agccgatgcc gggcacgcga tccagccgcg
     1381 ccgcgtgttt caattgcttg tgctgcgccg caatctgcgc ttcgatctgc tcgatgcgct
     1441 gatcgatgtc gtgcagtgcc tgcaactgca catcgagcag ccgctgcatc ggctgcggca
     1501 gttgcgcgtc gatgcttgcg cgctgtgctc gcaacgcttt cagcccgacg cggctgccgc
     1561 gtggcagcac aacaccgaac tcgtacaaca gcccgcgcag cgcattaatc gtcgcggtgc
     1621 tcacgctcac ccagtgcgcg cggctgcggt gcagcgtcag cacggcctgc tgatcctcgc
     1681 tcttgaccgg tacgcgccgg atgtcggtct gctgcgcggc aatccagatc gccctggcgt
     1741 cggcggcatc gtccttgttg ccgcgcacga aggcatgcac ctgccgcgcc ggcagcagct
     1801 cgacctggtg ccccagcgag cccagcagcc gcccccagtg gtgcgcgctg ccacatgctt
     1861 ccatcgctac gcgcaccggc tgcagccgtg cgaaatattc cgccagcttc gcacgcccaa
     1921 gcttcttgcg accgatctcg ccggtctgcg catcgaccca gtgcagttgc atcacgttct
     1981 tcgcaatgtc cacggcgtac gtcgtacgat ccatttcggg gcctccgtct cgagtggttg
     2041 cacaaatgca gctccactct ggcactcgat gccgttgacc gcgaggcccc accttttccc
     2101 cttcctaagc cttgcggcct cggcgtgtct cagcggtcgt tggcgagggg gaggcgtcca
     2161 taccatctcc tcaaggttgt ttagcacgat cgtgcgaaat atgggaaaag acaccgacct
     2221 tctcatagct tggtttctgc actggggact agcaggcggc ggcctggcgc gcggcaatca
     2281 gccgctcgta acaggcccag gcgcgctcgg cggcacgagg ctcgcgcagg aagcgcgcct
     2341 cgcgcatcag cgcgacgaag aggccgtcga tctcgaatac gagctgctcg gcatcggtat
     2401 cgggcctgaa gtggccggca tcgatggcct ggatcacggt gcgcttgagc gccgtgcgcc
     2461 agcctcggat gccttccagc agcgcgtcgc gcagcggccc ttcgcggtcg tcgaactcga
     2521 aggcgccggc gcaatagatg cagccgctgt gcatctcgac ttcgcggcag cgcttgagcc
     2581 acagctccgc gatggcgttg agccgcggca aaccgcgcgg ctcgcgcatg gccggcacga
     2641 acacatcctg caggaagcgc cggtcgtatt cctcgctcac cgccttctgc aaggcctcgc
     2701 gcgaacccac gcgggagaag acgccgctct tggacaggcc caggcgcttg gcgacctcgc
     2761 cgatcgacag gctttccagc ccatcggcgg ccgccatctg cagggcggtg tcgacgatgg
     2821 cggctagggt gaggccgctg cgctcggttt tggtttccat gttggtgggg agtttacgca
     2881 cgatcgtgcg aaattgcaaa agctgcacct tggcaacttt gtctcctaag ggtgctcagg
     2941 agtcgccccg caggggcgaa cccccatcgg gctatcgcaa aaccagtggc cgctatcgca
     3001 tcaagccgcg atccgcgcct ccaccccgcg cggaatcgcc gccagcaaac gcctcgtata
     3061 ttcctgctgc ggcgcatcga gcagcgcctg cgttgcgttg cgctcgacca cctcgccgtc
     3121 cttcatcacc agcacctcgt cggccatgaa gcgcaccact gcgaggtcgt ggctgatgaa
     3181 gatgtaggca agccccagct cgtcctgcag gtcgcgcagc aggttcagca cctgggcctg
     3241 cacgctcaca tccagcgccg agacggcctc gtcgagcacg atcacctcgg gctccaccgc
     3301 gatggcccgc gcgatcgcga cgcgctggcg ctggccgccc gagagctcgt gcgggtactt
     3361 ctccgcga
//
LOCUS       DASUCY010000007         2745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1462079, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000007 DASUCY010000000
VERSION     DASUCY010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1462079"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1587)
                     /locus_tag="VFV25_00125"
     CDS             complement(<1..1587)
                     /locus_tag="VFV25_00125"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY1391748.1"
                     /translation="MSKPIKVLVVEDSEDDTRLAMLALRRGGFAPTWRRVQDAEGLKK
                     AMAEECWDIVLSDFRLPDFTGVEALHIFRGSGLDIPFIFVSGTIGEETAVEAMKAGAS
                     DYVMKQSTARLAAVVRRELEQAVIRAQHRQAQIDLELTRDRYIDLYDFAPVGYLSLSG
                     EGSIQQLNLTGAELLGDSRDALLGQEFARFVAAGDLQRWSEHFMKAMHDGQKPQLELA
                     MQRADGSGFQAHLDCRRVLDRDASALVRLAMIDISDRKEAEAERRNVEAQLRHVQKME
                     SVGTLAGGIAHDFNNILGAILGNIQLTREEIGHGHPAQRSLEEIERASLRARNLVRQI
                     LTFSRHEPQQLVVQPLAPVVDEARKLLRATLPAGVELDARLDRAPLNVLADATLVQQV
                     LMNLCTNAWHALKPSGGHIHIGLRRAIINARGAARLGLARAGVYAHLWVRDNGCGMDA
                     MTRERIFEPFFTTKPVGQGTGLGLSVVHGIVTAHHGAIAVESVPEKGSCFHLYFPMVE
                     EASIQQRAVATALPASTANGE"
     gene            complement(1584..2039)
                     /locus_tag="VFV25_00130"
     CDS             complement(1584..2039)
                     /locus_tag="VFV25_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009668176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY1391749.1"
                     /translation="MQEQSKTILLVEDNPDDEALTLRAFKRSHLMNPISVARDGVEAL
                     DFVFGRGTHAGRASASLPALIILDLKLPKLDGLGVLKALRADARTRYLPVVILTSSKE
                     EQDLVSGYALGANSYVRKPVDFVEFLEAVKVLGMYWLMINEPPPESASL"
     gene            complement(1924..>2745)
                     /locus_tag="VFV25_00135"
     CDS             complement(1924..>2745)
                     /locus_tag="VFV25_00135"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY1391750.1"
                     /translation="IERKNEELAALNKEIEAFNYSVSHDLRAPLRSMDGFSLALLEDY
                     GEQLDDEGKDSLNRIRAASQRMGRLIDELLGLSRVTRTELAIRAVDLSPIAREIAESL
                     RRQSPQRQVQWVIDEGIVVQADRALMQIAMQNLLENAWKFTGKIENAVIRVGAVEHDG
                     VMDCFVADNGVGFDMAYADRLFGAFQRLHHENEFSGTGIGLAIVQRILRRHEGRIWVH
                     ASPGHGATFFFSLKGQAHARTEQDHPAGGGQPGRRSPDVAGLQAQPFDEPHLRGA"
BASE COUNT          424 a          948 c          907 g          466 t
ORIGIN      
        1 ttctccattt gcggtcgagg caggaagcgc ggttgccacg gcgcgctgct gaatggacgc
       61 ctcttccacc atcgggaagt acaggtggaa gcaactgccc ttctccggga cgctctccac
      121 ggcgatcgcg ccgtggtgcg cggtgacgat gccgtgcacg accgagagcc cgaggccggt
      181 accctgcccc acgggcttgg tggtgaagaa gggctcgaag atgcgctccc gcgtcatggc
      241 gtccatgccg cagccgttgt cccgcaccca caggtgcgcg tacacgccgg cccgcgcaag
      301 gcccagccgc gccgcgcctc tggcgttgat gatggcgcgc cgcaggccaa tgtgaatgtg
      361 gccgccgctg ggcttcaagg catgccaggc gttggtgcac aggttcatca gcacctgctg
      421 caccagggtg gcgtcggcca gcacgttcag gggcgcacgg tccaggcgcg cgtcgagctc
      481 cacgccagcc ggcagcgtgg cccgcagcag cttgcgcgcc tcgtcgacga cgggtgccag
      541 cggctgcacg acgagctgct gcggctcgtg gcggctgaag gtgaggatct gccgcacgag
      601 gttgcgcgcg cgcaggctgg cgcgctcgat ctcttcgagg ctgcgctggg ctggatggcc
      661 gtggccgatt tcctcgcgcg tgagctggat gttgccgagg atggccccga ggatgttgtt
      721 gaagtcgtgc gcgatgccgc cggccaatgt gccgaccgat tccatcttct gcacatggcg
      781 cagctgcgcc tcgacattgc gcctttcggc ctcggcctcc ttgcggtcac tgatgtcgat
      841 catcgccagg cggaccaggg ccgaagcgtc ccggtccagc acccgccggc agtcgagatg
      901 agcctggaag ccgctgccgt cggcccgctg catcgccagt tccagctgcg gcttttggcc
      961 gtcgtgcatg gccttcatga agtgctcgga ccagcgctgc agatccccgg cagccacgaa
     1021 gcgcgcgaac tcttgaccga gcagcgcgtc gcggctgtcg cccagcagct cggcgccggt
     1081 gaggttgagc tgctggatgc tgccctcccc ggacaggctg aggtagccca ccggcgcgaa
     1141 gtcgtacagg tcgatgtagc ggtcgcgcgt gagctcgagg tcgatctgcg cctgccggtg
     1201 ctgggcgcgg atgacggcct gttccagctc gcgccggacc accgcagcca ggcgcgccgt
     1261 gctctgcttc atcacgtagt cgctcgcgcc cgccttcatg gcctcaaccg cggtctcctc
     1321 cccgatcgtg cccgacacga agatgaaggg gatgtccagg cccgagccgc ggaagatgtg
     1381 cagcgcctcg acgccggtga agtcgggcag gcggaagtcc gacagcacga tgtcccagca
     1441 ctcctcggcc atggccttct tcaggccctc ggcgtcctgc acgcggcgcc acgtgggcgc
     1501 gaagccgccg cggcgcagcg caagcatcgc cagccgcgtg tcgtcttccg agtcctcaac
     1561 gacgaggacc ttgatcggct tgctcacagc gaggcgctct ccggcggcgg ctcgttgatc
     1621 atcagccagt acatgccgag caccttcacc gcttccagga actcgacgaa atccaccggc
     1681 ttgcgcacat agctgttggc cccgagcgca tagccggaga ccagatcctg ctcctccttc
     1741 gacgaagtga ggatgacgac cggcaggtag cgcgtgcgag cgtcggcgcg caaggccttc
     1801 agcacgccca ggccgtcgag cttgggcagc ttcagatcga ggatgatcag ggccggcagg
     1861 gatgcactgg cgcgcccggc gtgggtgccg cggccgaaca cgaaatccag agcctcgacg
     1921 ccgtcacgcg ccacggagat ggggttcatc aaatggctgc gcttgaaggc ccgcaacgtc
     1981 agggcttcgt cgtccgggtt gtcctccacc agcaggatgg tcttgctctg ttcttgcatg
     2041 tgcttgcccc ttgagactga agaagaaggt ggcgccgtgc ccgggcgagg cgtgcaccca
     2101 gatccgccct tcgtgccgcc gcaggatgcg ctgcacgatg gccagcccga tgccggtgcc
     2161 cgagaattcg ttctcgtggt gcaaccgctg gaaggcaccg aacagccggt cggcgtaggc
     2221 catgtcgaag cccacgccgt tgtctgcgac gaagcagtcc atgacgccgt catgctccac
     2281 cgcaccaacg cggatcaccg cgttctcgat cttgcccgtg aacttccagg cgttctccag
     2341 caggttctgc atcgcgatct gcatcagcgc gcggtccgcc tgcacgacga tgccctcgtc
     2401 gatcacccac tggacctgcc gctgcggcga ctgccgccgc agcgactcgg cgatctcgcg
     2461 cgcgatcggg ctcaggtcca ccgcacggat cgcgagctcg gtgcgcgtca cgcgcgacag
     2521 gccgagcaac tcgtcgatca gccgccccat gcgctggctg gccgcgcgaa tgcggttcag
     2581 gctgtccttg ccctcgtcgt ccagctgctc accgtagtcc tccagcaagg ccagcgagaa
     2641 gccatccatg ctgcgcagcg gcgcgcgcag atcgtgcgag acggaatagt tgaaagcctc
     2701 gatctccttg ttcagcgccg ccagctcctc gttcttgcgc tcgat
//
LOCUS       DASUCY010000008         4066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_433947, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000008 DASUCY010000000
VERSION     DASUCY010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433947"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1238)
                     /gene="htpG"
                     /locus_tag="VFV25_00140"
     CDS             complement(<1..1238)
                     /gene="htpG"
                     /locus_tag="VFV25_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HEY1391751.1"
                     /translation="MDKQTLSFQAEVKQLLYLVTHSLYSNQEIFLRELVSNASDACDK
                     LRFEALNNAGLYEDAPKLEVRLSYDKVARTITISDNGIGMSAQEAIEHLGTIAKSGTR
                     EFMGKLSGEQAKDAQLIGQFGVGFYSGYIVADRITVESRRAGLSPAEGVRWSSEGAGD
                     FEVETITRERRGTDVILHLREDAEDFLDSWRIKSIVGKYSDHISLPILMRKEEWDQDK
                     QEQVAKDEWETINKAAALWTRNKSDITDAEYKSFYEQLSYDATPPLAYTHNRVEGRSE
                     YTQLLYIPAKAPHDLWNRDKHGGVKLYVKRVFIMDDAEALMPAYLRFVKGVIDSADLP
                     LNVSRELLQESRDVKAIREGSTKRVLAMLEDLANNEDAGQKAKYAEFWREFGAVLKEG
                     VGEDFANRERLAKLLRFAST"
     gene            1454..2716
                     /locus_tag="VFV25_00145"
     CDS             1454..2716
                     /locus_tag="VFV25_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY1391752.1"
                     /translation="MSLTAPVSMLSHPASAPLYTLEGLRNADPILGLALLMIVALVAG
                     ELVHRASRLPRIVGQMLAGALASPLALRLLDQTELDPWKPLIDLAVGVLVFELGSRIR
                     PRWLLDNPWLSLSAACEAVFAGLIVSLVLHWMGMAPMSAVLAGVVAMSTSPVIAIAVV
                     HEAQPRGQVTERLLMLAALNSVLAVLALKAWRVAASAGAATPGFDWQPALANAVYVLC
                     GSFLLGAIVGLALERLARIVRGSGSLAVLQIGMVVLAALLATQWTLSPLLALLIAGVV
                     ARGRMGHGLTVEPQLGSAGAVLAVLLFLSMGLLSTLDDLLRVWPWVLAVLLARAAGKA
                     LGVFAVARPSGLSWRQAGALTLALQPMSNVALLLAATTYGWPDDIPAPDARLLQALLI
                     ATTLMQLSGPWWTQFGLRDVARESGNKH"
     gene            2727..3839
                     /locus_tag="VFV25_00150"
     CDS             2727..3839
                     /locus_tag="VFV25_00150"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HEY1391753.1"
                     /translation="MPLQAFSNSEALTLGVELELQLVGTHDFDLAPQAEDLLRETVKH
                     TGAWDIKPEITRSMIEIGTSIQREHGPLLAELRDMRNQVARAGRKLNIGIAGGGTHAF
                     QHWSEQQIFPTERFRYISELYGYLAKQFTVFGQHVHVGCENGDMALWLLHALSRYVPH
                     FIALSASSPYVQGQDTGFDSARLNSVFAFPLSGRAPFTRSWEEFGAYFDKMTATGVVQ
                     SMKDFYWDIRPKPEYGTIELRVCDTPLTVEKAAALACYLQTLCRYLREEKPFEPTEDD
                     YLVYTFNRFQACRFGLDGEIVDPKTKQRHKLRDDILRTLSRVDGHALDLRALDASNLI
                     RDSLFAGNDASWLREQRKGRTPLSAVVEGSAQRWMG"
BASE COUNT          591 a         1376 c         1362 g          737 t
ORIGIN      
        1 tgcgtcgacg cgaagcgcag cagcttggcc aggcgctcgc ggttggcgaa gtcttcgccg
       61 acgccctcct tcagcacggc accgaattcg cgccagaact cggcgtactt ggccttctgg
      121 ccggcgtctt cgttgttggc cagatcctcc agcatggcta gcacgcgctt ggtcgagcct
      181 tcgcggatgg ccttcacgtc gcggctttcc tgcagcagct cgcggctgac gttgagcggc
      241 aggtcggcgc tgtcgatcac gcccttcacg aagcgcaggt aggcgggcat cagcgcctcg
      301 gcgtcgtcca tgatgaagac gcgcttcacg tagagcttga cgccgccgtg cttgtcgcgg
      361 ttccacaggt catggggcgc cttggccggg atgtagagca gctgggtgta ttcgctgcgg
      421 ccctcgacgc ggttgtgcgt gtaagccagc ggcggcgtcg cgtcgtagct gagctgctcg
      481 tagaaggact tgtattcggc gtcggtgatg tcgctcttgt tgcgcgtcca cagcgccgcg
      541 gccttgttga tggtctccca ctcgtccttg gcgacctgct cctgcttgtc ctggtcccac
      601 tcctccttgc gcatcaagat gggcaaggag atgtggtcgg agtacttgcc gacaatggac
      661 ttgatgcgcc agctgtcgag gaagtcctcg gcgtcctcgc gcaggtgcag gatcacgtcg
      721 gtgccgcgcc gctcgcgcgt gatggtctcg acctcgaagt cgccggcccc ttcgctgctc
      781 cagcgcacgc cctccgcggg gctgagcccg gcgcggcgcg actcgacggt gatgcggtcg
      841 gccacgatgt agccgctgta gaagcccacg ccgaactggc cgatcagctg ggcatccttg
      901 gcctgctcgc ccgagagctt gcccatgaac tcgcgcgtgc cgctcttggc gatggtgccc
      961 aggtgctcga tcgcttcttg ggccgacatg ccgatgccgt tgtcgctgat cgtgatggtg
     1021 cgggctacct tgtcatagct caggcgcacc tccagcttgg gcgcgtcctc gtagaggccg
     1081 gcattgttca gcgcctcgaa gcgcagcttg tcgcaggcgt cggaggcatt ggagaccagc
     1141 tcgcgcagga agatctcctg gttggagtag agcgaatggg tcacgaggta aagcagctgc
     1201 ttgacctcgg cctggaagga aagggtttgc ttgtccatgg tgttcagtct gcgcggcgtc
     1261 ggcgccaagt ccaaattcgc gcagatggta tggtcaagtc gcgccgtttc aagggcggcg
     1321 aactcccatg ccatccggcg gtcatacccc cggccagcgc cgaagggttt cgtcacaagg
     1381 ccggccggcg cagcgcctaa agtcagtatc cgggcacgca gcccgtccaa cgtggggaac
     1441 aacaagtatg acgatgagcc tcaccgcccc ggtttccatg ctcagccacc cggcctcggc
     1501 gcctctgtat acgctggaag gcttgcgcaa cgccgatccg atcctgggcc tggccctgct
     1561 gatgatcgtc gccctggtgg ccggcgagct ggtgcatcga gccagccgcc tgccgcgcat
     1621 cgtcggccag atgctggccg gcgcgctggc cagccccttg gcgctgcgcc tgctggacca
     1681 gaccgagctc gacccctgga aaccgctgat cgatctggcc gtcggcgtgc tggtcttcga
     1741 gctgggcagc cgcatacgcc cgcgctggct gctcgacaac ccctggctga gcctgagcgc
     1801 cgcctgcgaa gcggttttcg ccgggctgat cgtgagcctg gtgctccatt ggatgggcat
     1861 ggcgccgatg tctgccgtgc tggcgggcgt cgtcgcgatg tcgacctcgc ccgtgatcgc
     1921 gatagccgtg gtgcacgagg cccagccgcg cggccaggtc accgagcggc tgctgatgct
     1981 ggccgcgctg aacagcgtgc tggcggtgct cgcgctcaag gcctggcgtg tggcggcttc
     2041 ggccggcgcg gccacgcccg gcttcgactg gcagccggcc ctggccaatg cggtctacgt
     2101 gctgtgcggc tccttcctgc tgggcgccat cgtcggcctc gcgctggaac gcctggcgcg
     2161 catcgtgcgc ggcagcggca gcctggcggt gctgcaaatc ggcatggtcg tgctggccgc
     2221 gctgctggcc acgcaatgga cgctgtcgcc gctgctggcc ctgctgatcg ccggcgtcgt
     2281 cgcgcgcggg cgcatgggcc acggcctcac cgtcgagccg cagctgggca gcgcgggcgc
     2341 cgtgctggcg gtgctgctct tcctgagcat gggcctgctg tcgacgctgg acgacctgtt
     2401 gcgcgtatgg ccctgggtgc tcgccgtgct gctggcgcgc gcagcgggca aggcgctggg
     2461 cgtgttcgcc gtggcccgcc ccagcggatt gagctggcgc caggccggcg ccttgacgct
     2521 cgcgctgcag ccgatgagca atgtcgcgtt gctgctggcc gccaccacct atggctggcc
     2581 ggacgacatc cccgcccccg acgcccgcct gctgcaggcc ctgctgatcg cgaccacgct
     2641 gatgcagctg agcggcccct ggtggaccca gttcggcctg cgcgacgtgg cgcgcgagtc
     2701 cggcaacaag cattaggagc atcgccatgc ccttgcaagc attcagcaac tcggaggccc
     2761 tgacgctggg cgtggagctg gagctgcagc tcgtcggcac ccacgacttc gacctcgcgc
     2821 cccaggccga ggacctgctg cgcgagacgg tcaagcacac cggcgcctgg gacatcaagc
     2881 ctgagatcac gcgcagcatg atcgagatcg gcacctcgat ccagcgcgag cacgggccgc
     2941 tgctggccga gctgcgcgac atgcgcaacc aggtcgcgcg cgctgggcgc aagctcaaca
     3001 tcggcatcgc cggcggtggc acgcatgcct tccagcattg gagcgagcag cagatctttc
     3061 ccaccgagcg cttccgctac atcagcgagc tctacggcta cctggccaag cagttcaccg
     3121 tgttcggcca gcatgtgcac gtgggctgcg agaacggcga catggcgctg tggctgctgc
     3181 atgcgctgtc ccgctacgtg ccgcatttca tcgcgctgtc tgcctcttcg ccctatgtgc
     3241 aggggcagga caccggcttc gactcggcgc ggctgaattc ggtgttcgcc tttccgctct
     3301 cgggccgcgc gcccttcacg cgcagctggg aggagttcgg cgcctacttc gacaagatga
     3361 cggccacggg cgtcgtgcaa tcgatgaagg acttctactg ggacatccgc cccaagcccg
     3421 agtacggcac catcgagctg cgcgtatgcg acacgccgct gacggtcgag aaggcggccg
     3481 cgctggcttg ctacctgcag accctgtgcc gctacctgcg tgaagagaag cccttcgaac
     3541 cgaccgagga cgactacctc gtctacacct tcaatcgctt ccaggcctgc cgcttcggcc
     3601 tggacggcga gatcgtcgac cccaagacca agcagcgcca caagctgcgc gacgacatcc
     3661 tgcgcacgct atcccgcgtc gacggccatg cgctggacct gcgcgcgctc gacgcctcca
     3721 acctgatccg cgattcgctc ttcgccggca acgatgcgag ctggctgcgc gagcagcgca
     3781 aggggcgcac gccgctcagc gcggtggtgg agggctcggc acagcgctgg atgggctgag
     3841 tctctttgct actctttctg taccgatatt caccaaatat agacgggtag atcgtcaatt
     3901 tccatttggt gcgtgctggg cggacgccgc aagcttgccc ggttctgctt cgcccgcgcc
     3961 cgagtgacgc tgcagcgcca gcgtcagcag ccagaccgcg acgatggcca gcgcgagcca
     4021 gaccagggcc aggcccaagg actgctggtc gtcgagacgg taggca
//
LOCUS       DASUCY010000009         3166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1285395, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000009 DASUCY010000000
VERSION     DASUCY010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1285395"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VFV25_00155"
     CDS             <1..487
                     /locus_tag="VFV25_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY1391754.1"
                     /translation="AWAEDAVSETIVAALEKPQSFAGRSQLKTWLVGILKHKVVDQMR
                     RNSRECALVAEDIDGDEFELLFKEDGHFRELPAEWGDPQAACRQQQFMVVLEACTEKL
                     PNQLGRVFLMREWLELETDEICKELGITSTNLWVMLHRARVRLRECLQLNWFGAKEAA
                     R"
     gene            484..690
                     /locus_tag="VFV25_00160"
     CDS             484..690
                     /locus_tag="VFV25_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEY1391755.1"
                     /translation="MRFLRRTCREAAALMLSRQERDLPWVERLALRIHLAACDACPRF
                     DAQVRFMQEAMRQWRHYSETEREG"
     gene            808..1734
                     /locus_tag="VFV25_00165"
     CDS             808..1734
                     /locus_tag="VFV25_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY1391756.1"
                     /translation="MPACHPLSLQGLLCLLFSAACALAPSLAAAQIQDHEAEAALIPI
                     LRGEASAYEHGEGVVADGKHAAELYCRAARLGDAESQFNLGWMYANGRGVERSDSMAA
                     FFFNAAAEQGYAQAVQMLSQVGGPPNYVPDCMREPPAPVAVATPAPVPRPPTSAAPKT
                     IGDLVKKMAPEYRVQPQFALAIIEAESNFNVAALSPKNAKGLMQLLPETATRFNVRNP
                     YDAAQNIRGGLAYLRWLLAYFEGDVILVAAAYNAGEATVERYRGVPPYLETRAYVARI
                     LHGCGALAYPYDASVTQPSAQLSLIRGRRGVM"
     gene            complement(1741..2148)
                     /locus_tag="VFV25_00170"
     CDS             complement(1741..2148)
                     /locus_tag="VFV25_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY1391757.1"
                     /translation="MTKMIFVSLPVTKLQTSITFYKALGFQQNPQFSDDASACMVWSE
                     AIYAMLLTHAKWRTFTERPLPPPGTSATMLSLAMDSRDAVDSMNAAAAAHGGQADVNP
                     VQDLGFMYSRDLADPDGHLWGAFWMDPAAIPQS"
     gene            complement(2169..>3166)
                     /locus_tag="VFV25_00175"
     CDS             complement(2169..>3166)
                     /locus_tag="VFV25_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017331930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1391758.1"
                     /translation="VVVLAGSLLLATGLALASLATSLVAFQLLFGVVVGISAAAIFAP
                     MMACVTGWFDTRRSLAVSLVSAGMGMAPMTMSPLAAWLVQTHDWRTSLQIIAALVAAI
                     MVPVALLVRRPPALAQPAAATVPPGAAHEQAMSVGQALRSAPFLILVLTSFLSCATHS
                     GPIFHTVSYAVTCGIPLMAAVSIYSLEGLAGMGGRLVFGLLGDRFGAARILVLALLAQ
                     AIGALGYVYARELGSFYTVAALFGFTYAGVMPLFAVIARENFPLRMMGTVIGGTTMAS
                     SLGMALGPVLGGLIYDTFSSYAWLFIGAFSLGIGAFLAALAFTRLPRRQTMAVVA"
BASE COUNT          579 a         1074 c         1036 g          477 t
ORIGIN      
        1 cgcctgggcc gaggatgcgg tgtccgaaac catcgtcgcg gcgctggaga agccccagtc
       61 ctttgcgggt cgctcccagc tcaagacctg gctggtcggc atcctcaagc acaaggtggt
      121 cgaccagatg cgccgcaaca gccgcgaatg cgcactcgtg gccgaggata tcgatggcga
      181 cgaattcgag cttctgttca aggaagacgg ccatttccgt gagttaccgg ccgaatgggg
      241 tgacccgcaa gccgcatgcc gccagcagca gttcatggtc gtgctcgaag cctgcaccga
      301 gaagctgccc aaccagctgg gccgcgtctt cttgatgcgc gaatggctgg agctcgaaac
      361 tgacgagatc tgtaaggaat tgggcatcac ctcgaccaac ctctgggtga tgctgcaccg
      421 cgcccgcgtg cgtttgcgcg aatgcctgca gctcaattgg tttggggcga aggaagctgc
      481 ccgatgagat tcctgcgacg cacctgccgc gaggcagccg ccttgatgtt gagccgccag
      541 gagcgcgacc tgccctgggt cgagcgcctg gccctgcgca tccacctcgc ggcctgcgat
      601 gcctgcccgc gcttcgacgc ccaagtgcgc ttcatgcagg aagccatgag gcaatggcgg
      661 cactacagcg agaccgagcg ggaaggctag atccctctgg agcccccctc gattgcgggg
      721 cgctgggcgg acaggcgatt gccccgcacc gacaggcgtg agattctttg gcggccaccc
      781 gccgcatcgc cggcctgcac tcccgccatg ccagcctgcc atccgctttc gcttcaggga
      841 ctgctgtgcc tgctgttcag cgccgcctgc gcgctggcgc ccagcctcgc cgcagcgcaa
      901 atacaagacc atgaggccga ggcggcgctg atcccgatat tgcgcggcga ggcctcggcc
      961 tatgagcatg gcgaaggcgt cgtcgcggac ggcaagcacg ccgccgagct gtattgccgg
     1021 gctgcaaggc tgggcgacgc cgagtcgcaa ttcaacctcg gctggatgta tgccaacggc
     1081 cgcggcgtgg agcgcagcga ttccatggcc gccttcttct tcaacgccgc cgccgagcag
     1141 ggctacgcgc aagcggtgca gatgctgagt caggtaggcg ggccgcccaa ctacgtgccg
     1201 gactgcatgc gcgagccgcc cgcccccgtt gccgtggcaa cgccggcgcc ggtgccgcgg
     1261 ccgccaacaa gtgcggcacc caagaccatc ggcgacctgg tgaagaagat ggcgcccgaa
     1321 taccgggtgc agccccagtt cgcgctggcc atcatcgagg ccgagtcgaa cttcaacgtg
     1381 gccgcactct cgccgaagaa cgccaagggc ctgatgcagc tgctgcccga gacggccacg
     1441 cgcttcaatg tccgcaaccc ttacgacgcg gcgcagaaca tacgcggcgg gctggcctat
     1501 ctgcgctggc tgctggccta tttcgagggc gacgtgatac tggtcgcggc cgcctacaac
     1561 gccggcgaag ccacggtgga gcgctaccgc ggcgtgccgc cctatctcga aacccgcgct
     1621 tatgtggcgc gcatcctgca cggctgcggt gcgctggcct atccctatga cgcgagcgtc
     1681 acccagccct ctgcacagct gagcctgata cggggacgcc gcggcgtgat gtaacggacc
     1741 tcagctttgc ggaatcgccg ccggatccat ccagaatgct ccccacaagt gtccgtcggg
     1801 gtcggcgagg tcgcggctgt acatgaagcc caggtcctgc accggattca catcggcctg
     1861 cccgccgtgg gccgcagcgg cagcattcat cgaatcgacg gcgtcgcggc tatccatggc
     1921 cagcgaaagc atcgtcgcgc tggtcccggg gggtgggagc ggccgctcgg tgaacgtgcg
     1981 ccacttggcg tgcgtgagca gcatcgcgta gatcgcctcg ctccacacca tgcaggctga
     2041 agcgtcgtcg ctgaattgcg ggttttgctg gaatcccagt gccttgtaga aggtgatcga
     2101 ggtttgaagc ttggtgacag ggagactgac gaaaatcatc ttagtcataa gggttccttg
     2161 gttagtgctc atgccaccac ggccatcgtc tgccgccgtg gcagccgcgt gaacgccagc
     2221 gctgccagga aggcgccgat gcccagcgag aacgcgccga tgaacagcca cgcatagctg
     2281 gaaaacgtgt cgtagatcag cccgcccagc acggggccca gcgccatgcc gaggctgctg
     2341 gccatcgtgg tgccgccgat caccgtgccc atcatgcgca gcggaaaatt ctcgcgcgcg
     2401 atcacggcaa acagcggcat tacgcccgca taggtgaagc cgaacaacgc ggcgacggtg
     2461 tagaagctcc ccagctcgcg tgcgtacaca tagcccagcg ccccaatcgc ctgggcgagc
     2521 agcgccagca ccaagatgcg cgcggccccg aagcggtcgc ccagcagccc gaacacgagt
     2581 cggccgccca tccccgcgag gccttccagg ctgtagatcg acacggcagc catgagcggg
     2641 atgccgcagg tgacggcgta gctcaccgtg tgaaagatcg gccccgagtg ggtggcgcag
     2701 ctcagaaagc tcgtcagcac caggatcagg aacggcgcag agcgcagggc ttgcccgaca
     2761 gacatggctt gctcatgcgc cgcgccaggt gggaccgtgg cagccgcggg ttgcgccagc
     2821 gcgggcgggc ggcgcaccag cagtgccacg ggcaccatga tggccgccac cagggccgcg
     2881 atgatttgca gcgacgtgcg ccagtcgtgc gtctgcacca gccacgcggc cagcggcgac
     2941 atggtcatcg gcgccatgcc catgccggcc gacaccagcg acactgccag gctccgccgc
     3001 gtgtcgaacc acccagtgac gcacgccatc atcggcgcga agatcgctgc cgcgctgatg
     3061 ccgacaacca cgccgaacaa cagctggaac gccacgagtg acgtcgccag gctggccagc
     3121 gcgagaccgg tggcgagcag caaggagcct gccaagacca ccacgc
//
LOCUS       DASUCY010000010         4066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_867786, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000010 DASUCY010000000
VERSION     DASUCY010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_867786"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..360
                     /locus_tag="VFV25_00180"
     CDS             202..360
                     /locus_tag="VFV25_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1391759.1"
                     /translation="MLYQPPSAARPYWDAMVKRMKMVFKAPIADADMPDIVDYLVKTY
                     GNEQQAAH"
     gene            457..723
                     /locus_tag="VFV25_00185"
     CDS             457..723
                     /locus_tag="VFV25_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1391760.1"
                     /translation="MPHDAPSAADSRSFSEANSLLAGKPARRSHPLLKRIATAVLLLL
                     ALPVFLLGLLVAVPVGLLVGAYGLIRQGLNAARQRQAPRRHTRD"
     gene            complement(739..1050)
                     /locus_tag="VFV25_00190"
     CDS             complement(739..1050)
                     /locus_tag="VFV25_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HEY1391761.1"
                     /translation="MILEVADIRIKPGQQAEFDRAIQHGIETVISKAAGFRGYKIKRG
                     IESPERYLLMIWWDTLENHMVDFRESDAFGQWRAIVGPYFAHPPVVEHFTLLASSAPR
                     G"
     gene            1191..3107
                     /locus_tag="VFV25_00195"
     CDS             1191..3107
                     /locus_tag="VFV25_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M61"
                     /protein_id="HEY1391762.1"
                     /translation="MQQTIRLARRALAAAALGMAFAAAASQGVPPVKDEPYPGVIALR
                     VDVTDLDHKIYRVQETLPVQPGRLTLLYPRWLPGTHGPYGETPRLAGLRVRAGSATLP
                     WVRDTDDPSAFHIEVPAGTSSLALEFQFLSPLTPDAGRVSMTREVLGVEWNDLILYPA
                     GHDARRIEVQPNLRLPEQWQQASALRAASAADSQGWLRYDRVSLETLVDSPVFAGRYI
                     KRVELDGPGAKRPVVLNLFADEAGQLEGTQAQIDAHRRLVQQADKLFGARHYAHYDFL
                     FALSDSFGGIGLEHHQSSENALNSTYFKDWNKAILGRELLPHEYTHSWNGKFRRPRDL
                     WTPNFNEPMHNSLLWLYEGQTQYWGRMLAARSGLVTPEQERDALANVAAVLEHRVGRS
                     WRNLQDTTNEGTMAVQRSTREWRDWQRSFDYYDEATLIWLDADTLIREKSGGKRSLDD
                     FARAFFGVDDGRVEPLLYDFDDIVHALNAVQPYDWADFLRKRLDGNGGGAPLDGLKRG
                     GWQLAYGDEASEFFKATEAEHKQCDLSYSLGLVIDTKDGKLAQVTWGSPAFNAGLAPG
                     MQLVAANWRAFDADRLKQTITAAKGGKQPIELLVKDGESYRTVSVDYHGGLRYPKLVR
                     NEGADRLSAILHAL"
     gene            3168..>4066
                     /locus_tag="VFV25_00200"
     CDS             3168..>4066
                     /locus_tag="VFV25_00200"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HEY1391763.1"
                     /translation="MTATSFETTRYDDPTAALQRVRAIYDASTATLRDALQRFVAGEN
                     FERHVRACYPFVRVHTDTVARADSRLSYGFVAGPGTYETTLTRPDLFGDYYDEQFHLL
                     LKNHGVALEVGVSNQPIPVHFSFAEHDYIEGSLNPERRLLMRDVFDLPNLGAMDDGIA
                     NGTYEPGPGEPRPLALFTAPRVDYSLHRLRHYTGTATEHFQNFVLFTNYQFYIDEFVR
                     LGRELMAQPPDQGALFGGAAMSDEYTAFVEPGNVGTRRAGTAPGPGDALGAPPPRMPQ
                     MPAYHLVRRDRAGITMVNIGVGPA"
BASE COUNT          684 a         1440 c         1317 g          625 t
ORIGIN      
        1 gcttgctggt ggcagtgccg gtgggtctgc tggtaggtgc ctatgggctg atcaggcaag
       61 ccttgaacgc ggcgcgccag gcgccccggc ggcacacgcg cgactgagct tcagcctctg
      121 ggcgccgagc tcaggcccag cgccctgccc ggctatgcca aggcaaaggc cagctgcgtc
      181 gcctgccatt cggccgaata catgctctat cagccgccca gcgccgcgcg cccctattgg
      241 gacgcgatgg tcaaacgcat gaagatggtg ttcaaggccc cgatcgccga cgccgacatg
      301 cccgacatcg tcgactacct ggtcaagacc tacggcaacg agcagcaggc cgcgcattga
      361 gcaggattgc acgctctcgc aagggagttg acaagccgtt acagctcgac gcgcgccagg
      421 ggcgctacat tggtctggtc agtacagcca gccgccatgc cccacgacgc tccttcagct
      481 gccgactcgc gctctttcag cgaggcgaat tcgctgctcg ccggcaagcc cgcgcgccgc
      541 agccacccgc tgctcaagcg catcgctacc gccgtcctgc tgctgctggc cctgcccgtc
      601 ttcctgctcg ggctgctggt ggcggtgccg gtgggtttgc tggtaggcgc ctacgggctg
      661 atcaggcagg gcctgaacgc ggcgcggcag cgccaggcgc cgcgacggca cacgcgggac
      721 tgagtgcccc aaaaggcctc agcctctggg cgccgagctc gccagcagcg tgaagtgctc
      781 caccacgggc ggatgcgcga aatagggccc gacgatcgcc cgccattggc cgaaggcgtc
      841 tgactcgcgg aagtccacca tgtggttctc cagcgtgtcc caccagatca tcagcagata
      901 gcgctcgggc gactcgatgc cgcgcttgat cttgtagccg cgaaagcccg cggccttgct
      961 gatcacggtc tcgatgccat gctgtatggc ccgatcgaat tcggcctgct ggccgggctt
     1021 gatgcggata tcggcgactt ccaggatcac gatgcggctc ccaagaaagc gccagtatga
     1081 cgccggccgc agcgctgcgg ccgaggccgg cacgcatggg ccgcacacaa aacggctcct
     1141 gcagagacgg atcttctcgg gtatcgtgca gccttttttt gggtccacgc atgcaacaga
     1201 ctatccgtct cgcgcgccgc gcgcttgccg ccgcggcgct gggcatggcc ttcgcggcgg
     1261 ccgcctcaca aggcgtgccg ccggtgaagg atgagcccta tccgggcgtc atcgcgctgc
     1321 gcgtcgatgt caccgatctg gatcacaaga tctatcgcgt ccaggaaacg ctgccggttc
     1381 agcccggtcg gttgactctg ctctacccgc gctggctgcc cggcacgcac ggcccctatg
     1441 gcgaaacgcc gcggctggcg ggcctgcgcg tacgcgcggg ctcggccacg ttgccctggg
     1501 tgcgcgacac ggacgatccg tcggccttcc acatcgaggt gcctgccggc acgagcagcc
     1561 tggcgctgga gttccagttc ctctcgccgc tgacgccgga tgccggccgc gtctcgatga
     1621 cacgcgaggt gctgggcgtg gaatggaacg atctgatcct ctacccggcc ggccacgatg
     1681 cgcggcgcat cgaagtgcag cccaacctgc gcctgcccga gcaatggcag caggccagcg
     1741 ccctgcgcgc cgccagcgct gcggacagcc agggctggct gcgctacgac cgcgtctcgc
     1801 tggaaacgct ggtggattcg ccggtgttcg cggggcggta tatcaagcgt gtcgaactcg
     1861 acgggcccgg cgcgaagcgg cccgtggtgc tgaacctgtt cgccgacgaa gcggggcagc
     1921 tcgagggcac gcaggcccag atcgacgcgc atcgcaggct cgtgcagcag gccgacaagc
     1981 tcttcggcgc gcgccactac gctcactacg acttcctgtt cgcgcttagc gacagcttcg
     2041 gcggcatcgg cctggagcat caccagtcga gcgagaacgc gctgaattcc acctatttca
     2101 aggactggaa caaggccatc ctcgggcgcg aactgctacc gcacgaatac acgcactcct
     2161 ggaacggcaa gttccgccgc ccacgggatc tgtggacgcc gaatttcaac gagccgatgc
     2221 acaacagcct gctgtggctg tacgaaggcc agacccagta ctggggccgc atgttggccg
     2281 cgcgcagcgg cctggtcacg ccggagcagg agcgcgatgc gctggccaat gtcgcggcgg
     2341 tgctcgagca tcgcgtcggc cgcagctggc gcaacctgca ggacacgacc aatgaaggca
     2401 cgatggccgt gcaacgcagc acccgcgaat ggcgcgactg gcagcgcagc ttcgactact
     2461 acgacgaagc cacgctgatc tggctcgacg ccgacacgct gattcgcgag aagtcgggcg
     2521 gcaagcgctc gctcgacgat ttcgcgcgcg ccttcttcgg cgttgacgac ggccgcgtcg
     2581 agccgctgct ctacgacttc gatgacatcg tgcacgcgct caacgccgtg cagccttacg
     2641 attgggccga cttcttgcgc aagcgcttgg acggcaacgg aggcggcgcg ccgctcgacg
     2701 gcctcaagcg cggcggctgg cagctggcct acggcgacga ggccagcgaa ttcttcaagg
     2761 ccaccgaagc cgagcacaag cagtgcgacc tgagctactc gctgggcctg gtgatcgaca
     2821 ccaaggacgg caagctcgcg caggtgacct ggggcagccc ggccttcaac gccggcctcg
     2881 cgcccggcat gcagctggtg gcggcgaact ggcgcgcatt cgatgctgac cgcctcaagc
     2941 agacgatcac cgccgccaag ggtggcaagc agccgatcga gctgctggtg aaggacggcg
     3001 agagctatcg caccgtgagc gtcgactatc acggtgggct gcgctatccc aagctggtgc
     3061 gcaacgaggg cgcagaccgg ctgagcgcga tcctgcacgc gctttgaccc tcgtcgccat
     3121 ggcgcctcgg tactgcgcca caatgaaaat cagaccccga ggctgccatg accgcgactt
     3181 ccttcgaaac cacacgctac gacgacccga ccgccgcgct gcagcgggtg cgcgcgatct
     3241 atgacgccag taccgcaacg ctgcgcgacg ccttgcagcg cttcgtcgcg ggcgagaact
     3301 tcgagcgcca cgtgcgcgcc tgctacccct tcgtgcgcgt gcacaccgac acggtggcgc
     3361 gcgccgactc gcgcctgagc tacggcttcg tcgccggccc cggcacctac gagacgacgc
     3421 tgacacggcc tgacctgttc ggcgactatt acgacgagca gttccacctg ctgctcaaga
     3481 accacggcgt ggcgctggag gtcggcgtca gcaatcagcc catcccagtg cacttctcct
     3541 tcgccgagca cgactacatc gaaggcagcc tgaaccccga gcggcgcctg ctgatgcgcg
     3601 acgtgttcga cctgcccaac ctcggcgcga tggacgacgg catcgccaac ggcacctacg
     3661 agcccggccc cggcgagccg cgacccctcg ccctgttcac ggccccgcgc gtggactact
     3721 cgctgcaccg gctgcgccac tacaccggca ccgcgaccga gcatttccag aacttcgtgc
     3781 tgttcacgaa ctaccagttc tacatcgacg agttcgtgcg cctgggccgc gagctgatgg
     3841 cgcagccgcc cgaccagggc gcgctgttcg gcggcgcggc gatgagtgac gaatacaccg
     3901 ccttcgtcga gcccggcaac gtcggcacgc gccgcgccgg caccgcgccc gggccgggcg
     3961 acgcgctggg cgccccgccg ccgcgcatgc cgcagatgcc ggcctatcac ctcgtccgcc
     4021 gcgatcgggc cggcatcacc atggtcaata tcggcgtcgg cccggc
//
LOCUS       DASUCY010000011         3184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1189077, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000011 DASUCY010000000
VERSION     DASUCY010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189077"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /gene="ccsB"
                     /locus_tag="VFV25_00205"
     CDS             <1..769
                     /gene="ccsB"
                     /locus_tag="VFV25_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEY1391764.1"
                     /translation="LTAVFYLWFEQRYGTRSLGAFVMLVVSAAVGFLLWYTVAREAQE
                     IQPLVPALQSWWMKLHVPANFIGYGTFSLAAMVALAYLLKTASTRALWTGLVGVPLLV
                     GVLPLAVFALVGLAPDSQAAVVKALRMAAGLVLFFSFAIWARRFVNPRTPEAVVMDDV
                     MYKAIAVGFAFFTIATILGALWAAEAWGGYWSWDPKETWALIVWLNYAAWLHMRLMKG
                     LRGSVAAWWALAGLLVTTFAFLGVNMFLSGLHSYGQL"
     gene            879..1877
                     /gene="msrP"
                     /locus_tag="VFV25_00210"
     CDS             879..1877
                     /gene="msrP"
                     /locus_tag="VFV25_00210"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HEY1391765.1"
                     /translation="MLIPKHRANGFVHPFSSEITPRSVYEQRRELLKLMAGGAAGAAL
                     AAWAARDARAQTPRPGKLAALSGARSAVPGAITMDKLTSYQDITSYNNFYEFGTGKDD
                     PVRNAGTLKTRPWTVSVEGAVNKPKVYDIDELLKLSPMEERIYRHRCVEGWSMVMPWV
                     GYSLAELIKRVEPQGSAKYVEFVTLADPKQMPYVRSSVLDWPYVEGLRMDEAMHPLAL
                     LSFGLYGEVLPNQNGAPVRIIVPWKYGFKSGKSLVKIRFVEKQPATAWVKAAAHEYGF
                     YSNVNPKVDHPRWSQATERRIGEDGLFARRRPTLMFNGYEAQVGQLYAGMDLVKNY"
     gene            1892..2527
                     /locus_tag="VFV25_00215"
     CDS             1892..2527
                     /locus_tag="VFV25_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HEY1391766.1"
                     /translation="MKRPSPLLLHPLAKPLLFAAALLPFAHLLYGAIADTLGANPAEA
                     LIRGTGDWTLRFLCLTLAITPLRQWTGQAALARFRRMLGLFTYFYVCLHLLSYSWLDK
                     GFYLGDIAHDIAKRPFILVGFAGFLLLTPLAATSLNRAVKALGARRWQALHRVVYAIA
                     LLGLLHFFWMRAAKHDFAQWSIYATVLGILLAWRLRPLLRGSILSLARSSP"
     gene            2591..>3184
                     /locus_tag="VFV25_00220"
     CDS             2591..>3184
                     /locus_tag="VFV25_00220"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY1391767.1"
                     /translation="MSQPSEPAAKPQPNATTAAPAGFGPDAQRKTCAALLAEADAAQR
                     SGDHRGGVQKAEAALAACLPGDDVLRAKALCLLAMHRWRLGQFEQAITATRDALPLLE
                     REDRETAARCEALCLMAVSYSELGLQEEALKQALMAFDLARERGDRLWMAWALNRIGI
                     CYERLGDPAQGERFLLQALDESLSLNDADIKFTSLNNL"
BASE COUNT          487 a         1076 c         1055 g          566 t
ORIGIN      
        1 gctcaccgcc gtcttctacc tttggttcga gcagcgctac ggcacgcgca gcctgggcgc
       61 cttcgtgatg ctggtggtca gcgccgcggt cggcttcctg ctgtggtaca cggtggcacg
      121 cgaggcgcag gagatccagc ccctggtgcc ggctctgcag agctggtgga tgaagctgca
      181 tgtgccggcc aatttcatcg gctacggcac cttctcgctg gccgcgatgg tggccctggc
      241 ctatctgctc aagacggcga gcacccgcgc gctgtggacc ggcctggtcg gcgtgccgct
      301 cttggtcggc gtgctgccgc tggccgtctt cgccctggtc ggcctggcgc ccgacagtca
      361 ggccgcggtg gtcaaggccc tgcgcatggc ggccgggctg gtgctgttct tctccttcgc
      421 gatctgggcg cgccgcttcg tcaacccgcg cacgccggag gcagtcgtga tggacgacgt
      481 gatgtacaag gccatcgcgg tcggcttcgc cttcttcacc atcgccacca tcctgggcgc
      541 gctatgggcc gccgaggcct ggggcggcta ctggagctgg gatccgaagg aaacctgggc
      601 gctgatcgta tggctcaatt acgcggcctg gctgcatatg cggctgatga agggcctgcg
      661 cggcagcgtt gcggcctggt gggcgctggc cgggctcctg gtcacgacct tcgccttcct
      721 gggcgtgaac atgttcctgt cggggctgca ttcttacggg cagctgtaag ccagggcagc
      781 atgcacgggt gtaaggtccg tgcaggctgt gcgactgagt gagcgggaac ccgttgacct
      841 tcccgccctc gaactcagct cacagcaaag gtcccgacat gctgatcccc aagcaccgcg
      901 ccaacggctt tgtccacccg ttttcctccg agatcacgcc gcgcagcgtc tacgagcaaa
      961 ggcgcgagct gctgaagctg atggccggcg gcgcggcggg cgccgcgctg gccgcctggg
     1021 cggcgcgcga tgcgcgggct caaacgccgc ggccgggcaa gctggccgct ctgtccggtg
     1081 cgcgcagcgc cgtgcccggc gcgatcacaa tggacaagct cacgtcctac caggacatca
     1141 cgagctacaa caacttctac gaattcggca ccggcaagga cgacccggtg cgcaatgccg
     1201 gcacgctcaa gacccggccg tggaccgtca gcgtcgaagg cgcggtcaac aagcccaagg
     1261 tctacgacat cgacgagctg ctcaagctca gccccatgga ggagcgcatc taccgccacc
     1321 gctgcgtcga gggctggtcc atggtgatgc cctgggtagg ctattcgctg gccgagctga
     1381 tcaagcgggt cgagccgcaa ggcagtgcca agtacgtcga gttcgtgacc ctcgccgacc
     1441 ccaagcagat gccctatgtg cgctccagcg tgctcgactg gccctatgtc gaaggcctgc
     1501 gcatggacga ggcgatgcac ccgctggcgc tgctgagctt cgggctctat ggcgaggtgc
     1561 tgccgaacca gaacggcgcg ccggtgcgca tcatcgtgcc gtggaaatac ggcttcaaga
     1621 gcggcaagtc cctcgtcaag attcgcttcg tcgagaagca gccggcgacc gcctgggtca
     1681 aggcggcggc gcatgaatac ggcttctatt ccaacgtgaa ccccaaggtc gaccatccgc
     1741 gctggagcca ggccaccgag cggcgcatcg gcgaggacgg cctgttcgcg cgccgccggc
     1801 cgacgctgat gttcaacggc tacgaggccc aggtggggca gctctacgcc ggcatggatc
     1861 tggtgaagaa ctactgatga ggtagcagca catgaagcgt ccttctccgc tcctgctgca
     1921 cccgctggcc aagccgctgc tctttgccgc cgcgctgctg cccttcgcgc atctgctcta
     1981 tggcgccatc gccgacaccc tgggcgccaa cccggccgag gcgctgatcc gcggcactgg
     2041 cgactggacg ctgcgcttcc tgtgcctgac gctggcgatc acgccgctgc ggcagtggac
     2101 gggccaggcg gcgctggcgc gcttccggcg catgctgggt ctgttcacct atttctatgt
     2161 ctgcctgcat ctgctgtcct acagctggct ggacaagggt ttctacctgg gcgacattgc
     2221 gcacgacatc gccaagcggc ctttcatcct ggtcggcttc gccggcttcc tcttgctgac
     2281 accgctggcg gcgacttccc tcaatcgggc cgtcaaggcg ctgggcgcca ggcgctggca
     2341 ggccttgcat cgcgtcgtct acgcgatcgc gctgctgggc ctgctgcatt tcttctggat
     2401 gcgcgcggca aagcacgatt tcgcacagtg gtcgatttat gcaacggttc tcggcatcct
     2461 gctggcctgg cgcttgcggc ccttgttgcg cggcagcata ctgagcttgg cacgctcctc
     2521 cccataaggg gggatggcgc agcgggcact aagcatgccc aatgcggcgt gttttttgga
     2581 cgaaagccgc atgtcccagc ccagcgagcc cgctgccaag cctcagccca acgccaccac
     2641 tgcggcgccg gccgggtttg gccctgacgc ccagcggaaa acctgcgcgg ctctgcttgc
     2701 cgaggccgat gcggcccagc gtagcggcga tcaccgcggc ggcgtgcaga aggccgaggc
     2761 ggcattggca gcttgtctac ctggcgatga cgtcctgcgg gccaaggcct tgtgcctgct
     2821 ggccatgcat cgctggcggc tcggacagtt cgagcaagcc atcaccgcca cccgcgacgc
     2881 cctgccgctg ctggaacgcg aagaccgcga gaccgccgcg cgctgcgagg ccctgtgcct
     2941 gatggctgtc tcctacagcg agctgggcct gcaggaagag gcgctgaagc aggccctgat
     3001 ggccttcgac ctcgcgcgcg agcgcggcga ccggctgtgg atggcctggg cgctcaatcg
     3061 catcggcatc tgctacgagc gcctggggga cccggcccag ggcgagcgct tcctgctgca
     3121 ggccctggac gaatccctaa gcctgaacga tgcggacatc aagttcacct cgctcaacaa
     3181 tctg
//
LOCUS       DASUCY010000012         2926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1478320, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000012 DASUCY010000000
VERSION     DASUCY010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478320"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..166
                     /locus_tag="VFV25_00225"
     CDS             8..166
                     /locus_tag="VFV25_00225"
                     /inference="COORDINATES: protein motif:HMM:NF021824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative nucleotidyltransferase substrate
                     binding domain-containing protein"
                     /protein_id="HEY1391768.1"
                     /translation="MGLDALELGRSNGRVIDVGKLSSLDRDLLKDTLNVVKRFRTMLR
                     QRYRLDML"
     gene            190..846
                     /locus_tag="VFV25_00230"
     CDS             190..846
                     /locus_tag="VFV25_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEY1391769.1"
                     /translation="MVTHTLSPPHWWRMLKREWLLYHLGDERFRFMWDPPPPHEWVAL
                     DCETTGLNVHRDQIVSIGAVCIAGTRIVTSERLELLVRPSRRMSDESVRVHGLLERDV
                     AEGMEPDEAMMCLMRFIGSRPLVGYYLEFDVAMIDRAIFPLLGMGLPQPKIEVSSMFY
                     EYKSRQLPPYQHGGNIDLRFESMMRELDLPLRAAHDALNDAVMAALAFIKLRDLLGRR
                     "
     gene            complement(1009..1257)
                     /locus_tag="VFV25_00235"
     CDS             complement(1009..1257)
                     /locus_tag="VFV25_00235"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEY1391770.1"
                     /translation="MWFGPMGGVSGWGGGWAWFGVLHMLLWWVFVALGIFVLFKSLGR
                     LPDAGSRADRALGILRERYARGEISREEFEQRKHDLTA"
     gene            complement(1516..2313)
                     /locus_tag="VFV25_00240"
     CDS             complement(1516..2313)
                     /locus_tag="VFV25_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY1391771.1"
                     /translation="MNGMHTVTTVLEAARPACVNGVALHAPGESLDEQALRQRACSEL
                     LRQAAESAGLLADDAPALDGVVSAAASEAIEALLDRQLQPPEPCEEACRRHYEAHRAD
                     YAHGERVQLRHVLFAVTPGVDLHALCRRAEALLLELRCADSQSAAFAQAAAQWSNCPS
                     GTQGGELGWLTKRDCVPEFAHEVFGSAEVGVLPRLVHSRFGLHVVEALAREAGEALPY
                     EQVRAAVAQSLRQQSYVTMLRQYLKLLAGQAVVEGVQLDGADSPLLQ"
     gene            complement(2326..>2926)
                     /gene="narI"
                     /locus_tag="VFV25_00245"
     CDS             complement(2326..>2926)
                     /gene="narI"
                     /locus_tag="VFV25_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HEY1391772.1"
                     /translation="GMLTPHFVYEPFIGAGAKQLVAMASGGVAGLLGFAGVSLLLHRR
                     LADARIRATSKTSDVVILVLLWLQLALGLGTIPLSAQHLDGGMMLRLAEWAQRIATFR
                     AGAAELMDGVGWVFKAHMVLGMTMFLVFPFTRLVHVWSGFGTLAYVLRPYQLARSRRL
                     NLPAGHIAPAVRPATMPAGVPHRVPEGVPAAAAQREARI"
BASE COUNT          505 a         1036 c          960 g          425 t
ORIGIN      
        1 gctcaaggtg ggacttgacg ctctagagct cggccgcagc aacggccggg tcatcgacgt
       61 cggcaagctc agcagtctcg accgtgactt gctcaaggac acgctgaacg tcgtcaagcg
      121 cttcaggacg atgctgcgcc agcgctaccg gctcgacatg ctgtgagccg cgcgcccgcg
      181 ggagtcccga tggtcaccca taccctgtcg ccgccgcact ggtggaggat gctcaagcgc
      241 gagtggctgc tctaccacct gggcgatgag cgctttcgct tcatgtggga cccgccgccc
      301 ccccacgaat gggtcgcgct ggactgcgag acgaccggcc tgaacgtgca ccgcgatcag
      361 atcgtctcga tcggtgccgt gtgcatcgcg gggacacgca tcgtcaccag cgagcgtctg
      421 gaactgctgg tgcgcccgag ccgccgcatg tccgacgaga gcgtacgcgt gcacggtctg
      481 ctcgagcggg acgtcgccga aggcatggag cccgacgagg cgatgatgtg cctgatgcgc
      541 ttcatcggca gccggcccct cgtcggctac tacctcgagt tcgacgtggc gatgatcgac
      601 cgggcgatct tcccattgct cggcatggga ctaccgcagc cgaagatcga ggtgtcgagc
      661 atgttctacg aatacaagag caggcagctg ccgccctacc agcatggcgg gaacatcgac
      721 ctgcgtttcg agtccatgat gcgcgaactc gacctgccgc tgcgcgccgc ccacgacgcg
      781 ctcaacgacg cggtgatggc ggcgctggcc ttcatcaagc tgcgcgatct gttggggcga
      841 cggtaaagcc gagagccgga ggccgcgacg ctgacggcat ctgatcggca gggagcgtaa
      901 gcgcgctgca ttcgtcagcg cccttcacgt cccaactcgc ccactctttg ctgccaggcc
      961 gtgcgagttg cctcgctgag cctatccacg gagccacacc aaaccggact atgcggtgag
     1021 atcgtgtttg cgctgctcaa attcctcgcg actgatctcg ccgcgcgcgt agcgctcgcg
     1081 caggatgccc agcgcgcggt cggcacgcga cccagcatcc ggcagcctgc ccagcgactt
     1141 gaacaacaca aagatgccga gtgcgacgaa gacccaccac agcagcatgt gcagcacgcc
     1201 gaaccaggcc caaccgccac cccacccact cacgccaccc atggggccga accacatgac
     1261 agtgctccgt gcgaatgatg aaccctcatt ctgatgccca cagcgagctg gcgcttgacc
     1321 tgcgtcagta ccgagtcgac aagaacgccg acccggaatc ccggtgatta agcccgggcg
     1381 cgttagtcgc aggaacacct tgagcagcgc cggcacgtgc caataggtag cggggggcga
     1441 tttatcgacc aaagtccgcg ccgggttggc tgcgaatcgg gatcgtgcgt ctcggcagat
     1501 gcgtctgcgc tcgcgctatt gcagcagcgg cgagtcggcg ccgtcgagtt gtacgccttc
     1561 caccaccgcc tgcccggcca gcagcttcag gtactgccgc agcatagtga cgtaggactg
     1621 ctggcgcagc gactgcgcca ccgcggcgcg cacctgctcg tagggcagcg cctctccggc
     1681 ttcgcgagcc agtgcctcca ccacatgcag gccgaaacgg ctatgcacca ggcgcggcag
     1741 cacgccgacc tccgcgctgc cgaacacctc gtgcgcgaac tcgggcacgc aatcgcgctt
     1801 cgtcagccag cccaactccc cgccctgcgt gccgctcggg cagttggacc attgcgcggc
     1861 cgcttgcgcg aaggccgcgc tctgggagtc ggcacagcgc aactccagca gcagcgcctc
     1921 ggcgcgccgg cacagcgcgt gcagatcgac gcccggcgtc accgcgaaca gcacatggcg
     1981 cagctgcacg cgctcgccat gcgcatagtc ggcgcggtgc gcctcgtagt ggcggcggca
     2041 ggcctcctcg catggctcgg gcggctgcag ctggcggtcg agcaaagcct cgatggcctc
     2101 gctggccgcg gcgctgacca caccgtccag ggcgggcgca tcgtcggcca gcagcccagc
     2161 agactcggct gcctggcgca gcagttcgct gcaggcgcgc tggcgcagcg cttgctcgtc
     2221 gagggactcg cccggcgcgt gcagggctac cccgttgacg caggccgggc gggccgcctc
     2281 gagcaccgtg gtcacggtgt gcatgccatt catgccggcg ccccttcaga tgcgcgcttc
     2341 gcgctgtgcg gcagcagcgg ggacaccctc ggggacccga tgcggcacgc ccgccggcat
     2401 cgtggccggc cgcactgccg gcgctatgtg gccggccggc aagttgagcc ggcggctgcg
     2461 cgccagctgg tagggccgca gcacataggc cagcgtgcca aagccgctcc acacgtgcac
     2521 gaggcgggtg aaggggaaca ccaggaacat cgtcatgccc agcaccatgt gggccttgaa
     2581 cacccagccc acgccgtcca tcagctccgc cgcgccggcg cggaaagtgg cgatgcgctg
     2641 cgcccactcc gccagccgca gcatcatgcc gccgtccaga tgctgggcgg acagcgggat
     2701 ggtgcccagc cccagcgcaa gctgcagcca cagcagcacc aggatcacga cgtcgctggt
     2761 cttggaggtg gcgcggatgc gcgcgtcggc aagcctgcga tgcagcagca gcgacacgcc
     2821 ggcaaagccc agcagacccg ccacgccgcc cgaagccatg gcgaccagtt gcttggcgcc
     2881 ggcgccgatg aagggctcgt agacgaaatg cggcgtgagc atgccc
//
LOCUS       DASUCY010000013         2519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_369828, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000013 DASUCY010000000
VERSION     DASUCY010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369828"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1737
                     /gene="edd"
                     /locus_tag="VFV25_00250"
     CDS             <1..1737
                     /gene="edd"
                     /locus_tag="VFV25_00250"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HEY1391773.1"
                     /translation="ATREPSMDRMGCANLAHAVAGIPLDDRFKVVTQRAPNIGIVTAY
                     NDMLSAHTPLQGYPALIKDEARKLGATAQVAGGVPAMCDGETQGTSVMELSLFSRDVI
                     AMATAVALSHDMFDAALMLGVCDKIVPGLLIGALQFGHLPTVFVPAGPMPSGLPNKEK
                     ARVREQAAQGLVGREGLLEAELKSYHSPGTCTFYGTANSNQMLLEAMGLHVPGTAFVA
                     PGGALRDELTREAARTVLGIIKSRRFAPIGQIVDERAIVNAMVALLATGGSTNHLIHW
                     VAVAHSAGIVIDWNDFSALSDVVPLLAHVYPNGSADVNQFQAAGGTGLIIRELLDAGF
                     MHEDVLTVREGGLREYTREPALADSSVAGTALAWHDAGDSKDEAIVRPVARPFSATGG
                     LKLLQGNLGRSVIKVSAVPDDRHVIEAPARVFDSQEALHAAFSAGELERDVVCVVRWQ
                     GPQANGMPELHKLTPPLAVLQGKGFKVALVTDGRMSGASGKVPAAIHVSPEAAAGGPL
                     AKVRDGDVIRLDAVTGELQVLVAEAEWAARQADVMPADLRRHNGIGMGRQLFTGMRRH
                     ALTAEEGALSWM"
     gene            1740..2390
                     /gene="eda"
                     /locus_tag="VFV25_00255"
     CDS             1740..2390
                     /gene="eda"
                     /locus_tag="VFV25_00255"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEY1391774.1"
                     /translation="MNVSGKLTALQVMQDAPVIPVIVLTDVKQAVSLARALVAGGIRM
                     LEVTLRTPQALACIEAIARDVPEAVTGAGTVRSAADAQACAMAGARFAVSPGYTHAVG
                     QACRDAGLPLLPGVATGSEIMMAQEDGLTELKFFPAMQAGGPAMLKAWGGPFGDVRFC
                     PTGGVSMANAAEFLALSNVVCVGGSWLTPGEVLARGDWAQVTALAQQACLLQRQAA"
BASE COUNT          402 a          808 c          894 g          415 t
ORIGIN      
        1 gccacacgcg agcccagcat ggaccgcatg ggctgcgcca atctggcgca tgccgtggcc
       61 ggcattccgc tggatgaccg cttcaaggtc gtgacccagc gggcccccaa catcggcatc
      121 gtcacggcct acaacgacat gttgtcggcg cacacgccgc tgcaaggcta ccccgcactg
      181 atcaaggacg aagcgcgcaa gctgggcgcc acggcgcaag tggcgggcgg cgtgcccgcc
      241 atgtgcgacg gcgagacgca aggcaccagc gtcatggagc tgagcctgtt cagccgcgac
      301 gtgatcgcca tggccacggc cgtggcgctg tcgcacgaca tgttcgatgc cgcgctgatg
      361 ctgggggtgt gcgacaagat cgtgccgggc ctgctgattg gcgcgctgca gttcggccat
      421 ttgcccacgg tgtttgtgcc cgccgggccc atgccctcgg gcctgcccaa caaggaaaaa
      481 gcccgggtgc gcgagcaggc cgcccagggg ctggtcgggc gcgaaggctt gctcgaggcc
      541 gagctcaagt cctaccacag cccgggtacc tgcacgtttt acggcacggc caacagcaac
      601 cagatgctgc tcgaagccat gggtctgcat gtgcccggca ccgcgtttgt ggcgcccggc
      661 ggtgccctgc gtgacgagtt gacgcgcgaa gccgcgcgca cggtgctggg catcatcaag
      721 tcccggcggt ttgcgcccat cggccagatc gtcgatgaac gcgccatcgt caacgccatg
      781 gtggccctgc tggccaccgg cggctcgacc aaccacctga ttcactgggt cgcggtggcg
      841 cattcggccg gcattgtgat tgactggaat gatttttcgg ccctgtccga tgtggtgccc
      901 ctgctggccc atgtgtatcc caacggcagc gccgacgtga accagtttca ggcggccggc
      961 ggcaccgggc tgatcattcg cgaattgctt gacgccggtt tcatgcacga agacgtgctg
     1021 acggtgcggg aaggcgggct gcgcgaatac acccgcgagc ccgcgctggc tgattcctcc
     1081 gtggcgggca cggcgctggc ctggcatgac gcgggcgact ccaaagacga agcgattgtg
     1141 cggcccgtcg cccggccgtt cagcgccacc ggcgggctca agctgctgca gggcaacctg
     1201 ggccgcagcg tcatcaaggt gtcggccgtg cccgatgacc ggcatgtgat tgaagcgccg
     1261 gcccgcgtgt tcgattcgca ggaggccttg catgcggcct tcagtgccgg cgagctggag
     1321 cgtgatgtcg tgtgcgtggt gcgctggcaa ggcccgcagg ccaacggcat gccggagctg
     1381 cacaagctca cgccgccgct ggcggtgttg cagggcaagg gcttcaaggt ggcgctggtc
     1441 accgatggcc gcatgagcgg cgcatcgggc aaggtaccgg ccgccatcca tgtctcgccg
     1501 gaggcggccg cgggcggccc gctggccaag gtgcgggatg gcgacgtgat ccgcctcgat
     1561 gcggtcaccg gcgagttgca ggtgttggtg gccgaggccg agtgggccgc gcggcaggcg
     1621 gatgtgatgc ctgccgactt gcgccggcac aacgggatcg gcatgggccg tcaattgttt
     1681 accggcatgc gccgccacgc gctgacggcc gaagaaggag ctttatcgtg gatgtgaata
     1741 tgaatgtcag cggcaaactg accgccttgc aggtcatgca ggatgcgccc gtgattcccg
     1801 tgattgtgct gaccgacgtc aagcaggccg tgtcgctggc gcgcgccctg gtggcgggcg
     1861 gcatccgcat gctggaagtc accctgcgca cgccgcaggc gctggcctgt attgaagcca
     1921 ttgcgcgcga cgtacccgag gccgtaaccg gcgccggcac cgtgcgcagt gccgccgacg
     1981 cccaggcctg cgccatggcc ggcgcccgtt ttgcggtgag cccgggctac acccatgcgg
     2041 tgggccaggc ctgccgtgat gccggtttgc ccctgttgcc cggcgtggcc acaggcagcg
     2101 agatcatgat ggcgcaggaa gacgggctga ccgaactcaa gtttttcccg gccatgcaag
     2161 cgggcggccc ggccatgctc aaggcctggg gcgggccgtt tggcgatgtg cgcttttgcc
     2221 ccacgggggg agtgtccatg gccaatgccg cagaatttct ggcgctgtcc aatgtggtgt
     2281 gcgtgggcgg ctcttggctc acgcccggtg aggtgctggc gcggggcgac tgggcgcagg
     2341 tgacggcgct ggcgcagcag gcctgcctgc tgcagcggca ggctgcttga gccggggagc
     2401 gctcaaaccc ggaaggttat gagcgaaatc ggcctctagc ccattaggaa tgggcgcaag
     2461 aagctattaa aacaatagca aacgccgcaa gccttagcgc aagccagccc caccgccat
//
LOCUS       DASUCY010000014         5389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_401937, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000014 DASUCY010000000
VERSION     DASUCY010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 5389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5389
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401937"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VFV25_00260"
     CDS             <1..516
                     /locus_tag="VFV25_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY1391775.1"
                     /translation="IRRLGPADLSAYKQFRDQALLRHPDAFTSDHETERARSPESYLG
                     RLGLSDSLGGSFLLGAWDKEQLVGSVCLERETRQKTRHTAWLVGMMVAESHSRRGIGR
                     ALVEQLIREARAAAGLQMIMLSVTASSERAVRLYESAGFKRYGLLPRAIRVVGAGGER
                     YYDKAFLLLQL"
     gene            complement(524..985)
                     /gene="rnhA"
                     /locus_tag="VFV25_00265"
     CDS             complement(524..985)
                     /gene="rnhA"
                     /locus_tag="VFV25_00265"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEY1391776.1"
                     /translation="MTTTAQAKQTPDVVIYTDGACKGNPGPGGWGVWMKSGSHEKELH
                     GGEASTTNNRMELTAVIEALSALKRPCHVLIYTDSEYVRKGITEWIHGWKTRGWKTAD
                     KKPVKNAELWRKLDELAAGHRVDWRWVKGHAGDPGNERADALANRGAARFM"
     gene            complement(982..1779)
                     /locus_tag="VFV25_00270"
     CDS             complement(982..1779)
                     /locus_tag="VFV25_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY1391777.1"
                     /translation="MPAIINLHSWLQTPPGRYLLTWEQARLDKAVADVFGFHALQLGL
                     PELDALQGNRMPHRWLALDRNGESLPPEAATSADGPAPRAVALFCDSDALPFPSQSLD
                     LVVLPHTLELARDPHHALREVERVLVPEGRVVIVGFNPASLWGLRQRWGRLCMSLGLG
                     SLFLPDAGEFIGYWRLRDWLRLLSFEIDAGSFGCYRPALRSQRWLERFAWTEHAGDRW
                     WPVFGAVYFLSAVKRVRGMRLVGSPRKQRAKPLAAPAVATNKHKEAN"
     gene            2194..3651
                     /locus_tag="VFV25_00275"
     CDS             2194..3651
                     /locus_tag="VFV25_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY1391778.1"
                     /translation="MSVAAAQSAASAPAAAEEPAKNAAGAPTDPLRPDAPVNLDDEAA
                     RTDLWVRIRHGFAMPDLDNDLVHEREQWYADRPDYVQRMTERGGRYLFHVVEELDKRG
                     MPTELALLPFIESAFNPQAMSVAKASGMWQFVPATGKHYALKQNVFRDDRRDVLASTR
                     AALDYLQRLHDMFGDWQLALAAYNWGEGNVNRAIARNQRAGLPTDYQSLSMPAETRLY
                     LPKLQAVKNIVSHPEAFGLSLPPLANHPYFLRVPIQRDIDVAVAVQLAEVPMEEFKTL
                     NPSLNKPVILAAGTPQLLLPYDNASLFLRNLEEHKGPLASWTAWIVPRTMRPAAAAKQ
                     VGMSESMLREINRIPPRMLVRVGSTLLVPRSEKRAHDVSEQIADNAALSLAPDGPATR
                     RVALRAGKADSVASIARRYHVSSAQVAQWNKVASDARFKHGQTIVVYVASRKHAGRHL
                     ASSGGAAAHTRHHASRTAAKAGNGRRARVQASAAN"
     gene            complement(3698..4291)
                     /gene="recR"
                     /locus_tag="VFV25_00280"
     CDS             complement(3698..4291)
                     /gene="recR"
                     /locus_tag="VFV25_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEY1391779.1"
                     /translation="MSAPEPSLSALIEALRRLPGVGVKSAQRMAFHLLQHDRAGAQLL
                     AQSLQAAVANVRHCERCHTFTEAPVCSTCLDPSRDRKQLCVVETPADQAAVERTGSFH
                     GLYFVLMGRLSPLDGVGVRDIGITQLLERACDGEVQEVIVATSFTAEGEATAHVIAEA
                     LKARGLTVTRLARGVPVGSELEYVDLGTIAHALVERR"
     gene            complement(4402..4728)
                     /locus_tag="VFV25_00285"
     CDS             complement(4402..4728)
                     /locus_tag="VFV25_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEY1391780.1"
                     /translation="MMKGQLAGLMKQAQAMQDNLKRAQEELAQIEVEGQSGAGLVKVT
                     MTCKHDVKRVAIDPSLLADDKDMLEDLVAAAFNDAVRRAEEVSQEKMGKLTAGMPLPP
                     GMKFPF"
     gene            complement(4745..>5389)
                     /locus_tag="VFV25_00290"
     CDS             complement(4745..>5389)
                     /locus_tag="VFV25_00290"
                     /inference="COORDINATES: protein motif:HMM:NF023592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau C-terminal
                     domain-containing protein"
                     /protein_id="HEY1391781.1"
                     /translation="SAAAARSAALRAPAPASRSSAASAAATDAEIPPWVDAPMATEIV
                     AAEVVPRPARAALSSAAIAAVPVDAEPEALSSADIALGDRWSALVRSLCEAGAIAALV
                     RELAMQAQCVAVSQQGLQGDMEVWRLRVERETLRGGSHRERLESAIAQALKQPVRLEL
                     EAGATTDSPAQRDAAQRERRQRAAEELIQNDPQVIALLQQYKSARIVPGSIKPI"
BASE COUNT          874 a         1912 c         1736 g          867 t
ORIGIN      
        1 atccgccggt tggggccggc cgacctctcg gcctacaagc agttccgcga tcaggccctg
       61 ctgcggcacc cggatgcctt cacctccgac cacgagaccg agcgtgcccg ctcgccggaa
      121 agctacctgg gccgactggg cctgagcgac agcctcggcg gcagcttcct gctcggcgcc
      181 tgggacaaag agcagctggt cggctcggtc tgcctcgaac gtgagacacg ccagaagacg
      241 cgccacaccg cctggctggt tggcatgatg gtggccgaat ctcacagccg ccgcggcatc
      301 ggccgcgcac tggtagagca gctgatacgc gaggcgcgcg cagccgccgg cctgcagatg
      361 atcatgctca gcgtgaccgc ctccagcgag cgcgcggtgc ggctatacga aagtgccggt
      421 ttcaagcgct acggcctgtt gccgcgcgcc atacgcgtgg tcggcgccgg cggcgagcgc
      481 tattacgaca aggccttcct gttgttgcag ctgtgaccgg atatcacata aagcgcgcgg
      541 cgccccgatt ggccagcgca tcggcgcgct cgttgcccgg atcgcccgca tggcccttga
      601 cccagcgcca gtcgacgcgg tgaccggcgg cgagctcgtc cagcttgcgc catagctctg
      661 cgttcttcac cggcttcttg tcggcggtct tccagccgcg cgtcttccag ccgtggatcc
      721 actcggtgat gcccttgcgc acgtactcgc tgtccgtata gatcagcaca tggcaaggcc
      781 gcttcagcgc ggacaaggcc tcgatcaccg cggtgagttc catgcgattg ttggtggtgc
      841 tggcctcgcc gccatgcagc tccttctcgt ggctgccgct cttcatccaa acgccccagc
      901 ctccggggcc gggattgccc ttgcaggcgc cgtccgtgta gatcaccacg tcgggcgtct
      961 gcttcgcttg cgcggtcgtc gtcaattcgc ttccttgtgt ttgttcgtcg ccaccgcggg
     1021 cgcagccagc ggcttggccc gctgcttgcg tggcgagccc accagccgca tgccgcgcac
     1081 gcgcttcacg gcgctgagaa agtagaccgc accgaacacc ggccaccagc ggtccccggc
     1141 gtgctccgtc caggcgaagc gttccagcca gcgctgcgat ctcagtgcag gacggtagca
     1201 gccgaagctg ccggcatcga tctcgaagct cagcaggcgc agccagtcgc gcagccgcca
     1261 atagccgatg aactcgccgg cgtcgggaag gaagaggctg cctagcccca agctcatgca
     1321 aagccggccc caccgctggc gcaggcccca aaggctggcc ggattgaagc cgacgatcac
     1381 gacccgccct tccggcacca gcacgcgctc gacctcgcgc aaggcatggt gaggatcgcg
     1441 cgccagttcc agcgtatgcg gcagcaccac aagatcgagg ctctggctgg gaaagggcaa
     1501 ggcgtcggaa tcgcagaaca gcgcgacggc gcgcggagcc gggccgtcgg ccgatgttgc
     1561 tgcttcgggc ggaagcgact cgccgttgcg atccagggcc agccagcgat gcggcatgcg
     1621 attgccctgc agggcatcca gctccggcag gccgagctgc agcgcgtgaa agccaaagac
     1681 atcggccacc gccttatcca gccgggcctg ctcccaggtc agcaggtaac ggccgggggg
     1741 tgtttgtaac caggagtgca aatttataat tgcgggcatt tggtcacgaa agtcttgctc
     1801 tgctcgcctc attccaacaa gcacatcggt atgctgcaca acggacctgg tgccgccaat
     1861 aggtctgata ttggctggtg cagcatcggc gctcgaagaa gcgccggctg aaaagatgtg
     1921 gagcctgcgc tcgatgtggt cggcattcta gtgacccgac ctcgaagcga ggcgaacggt
     1981 gccgccgtgt ggatgtcggc ggtacagcgt cccaggaaga acaactcgat gatgaccctt
     2041 tcatctttga cggctcgtct gccgttcaga cccgtccttg caggcctgct gctgcccttg
     2101 ctgctggctg cctgcgccac caacaaaccc ccgacttctc ccaatccctt gcctgtcgcg
     2161 gcagcggcct ccgccgcatc ggcgccggac ggcatgtccg tcgccgccgc gcagtccgcg
     2221 gcctcggcac ccgctgccgc cgaagagccg gccaagaacg cggcaggcgc gcccaccgac
     2281 ccgctgcggc ccgatgcgcc ggtcaatctc gacgatgaag cggcgcgcac cgatctgtgg
     2341 gtgcgcatac gccacggctt cgcaatgccc gatctcgaca acgacctcgt gcatgagcgc
     2401 gagcagtggt atgccgaccg tcccgactat gtgcagcgca tgaccgagcg cggcggccgc
     2461 tatctcttcc acgtcgtcga ggagctcgac aagcgcggca tgcccaccga gctcgccctg
     2521 ctgcccttca tcgagagtgc cttcaacccg caggccatgt cggtggccaa ggcctcgggc
     2581 atgtggcagt tcgtgccggc caccggcaag cattacgcgc tcaagcagaa cgtgttccgt
     2641 gacgaccgcc gcgacgtgct ggcctcgacc cgtgccgcgt tggactacct gcagcgcctg
     2701 cacgacatgt tcggcgactg gcagctcgcg ctggccgcct acaactgggg cgaaggcaat
     2761 gtgaaccgag ccatcgcccg caatcagcgc gccggcctgc ccaccgatta ccagagcctc
     2821 agcatgccgg ccgagacccg gctctacctg cccaagctgc aggcggtgaa gaacatcgtc
     2881 agccacccgg aggccttcgg cctcagcctg ccgcccctcg ccaatcaccc ctatttcctg
     2941 cgcgtgccca tccagcgcga tatcgacgtg gccgtggccg tccagctggc ggaagtgccc
     3001 atggaggagt tcaagacgct gaacccctcc ttgaacaagc cggtaatcct ggccgctgga
     3061 acgccccagc tgctgctgcc ctacgacaac gccagcctct tcctgcgcaa cctcgaagag
     3121 cacaagggcc cgctcgcgag ctggaccgct tggatagtgc cgcgaaccat gcgcccggcc
     3181 gctgctgcca agcaggtggg catgagcgag agcatgctgc gcgaaatcaa tcgcatcccg
     3241 ccacgcatgc tcgtcagggt gggctccaca ctgctggtgc cgcgcagcga aaagcgggct
     3301 catgacgtgt ccgagcaaat cgccgacaac gccgcgctgt ccttggctcc ggatgggcct
     3361 gcgacgcgcc gcgtggccct gcgtgcgggc aaggccgaca gcgtggcctc gatcgctcgc
     3421 cgctaccacg tcagctcggc ccaggtcgcg cagtggaaca aggtggcgag cgacgcccgc
     3481 ttcaagcatg ggcagaccat cgtggtctat gtcgcaagca ggaagcacgc tggccgccat
     3541 ctcgcgagtt ccggcggcgc tgccgcgcat acccgtcacc acgcatcgcg caccgctgcc
     3601 aaggcaggca acggccgcag ggcacgcgtg caggccagcg cggcgaacta agcttcgctc
     3661 tctcttctgc accgctgcgc cgtgcgcgca gcggcattca gcggcgctcg accagtgcgt
     3721 gggcgatggt gcccagatcg acgtattcga gctcgctgcc taccggcaca ccgcgcgcca
     3781 gccgcgtgac cgtgaggcca cgcgctttca gcgcctcggc gatcacatgg gccgtggcct
     3841 cgccctcggc cgtgaaactg gtcgcgacga tcacctcctg cacttcgccg tcgcaggcgc
     3901 gctccaggag ctgggtgatg ccgatgtcgc gcacgcccac gccgtccagc gggctcagtc
     3961 gccccatcag cacgaagtac aggccgtgaa agctgccggt gcgctccacg gcagcctggt
     4021 cggcaggggt ctcgaccacg cagagctgct tgcgatcgcg cgacgggtcg aggcaggtcg
     4081 aacaaaccgg cgcctcggtg aaggtatggc agcgctcgca atgccgcacg ttggccaccg
     4141 ccgcctgcag cgattgcgcc aacagctgcg cgccagcacg gtcatgctgc agcagatgaa
     4201 aagccatgcg ctgcgccgac ttcacaccca caccgggcaa ccggcgcaag gcctcgatca
     4261 gcgcggaaag tgacggctcc ggagcgctca cggcagcacg tcaagccgcg atccaagcag
     4321 gcgccgcgga tccggctccg ccggtccgcc agcgccgccc ccttgagggg gagcggcgaa
     4381 gccgctacgg gggggagcca tctagaacgg gaatttcatc cccggcggca gcggcatgcc
     4441 ggcggtcagc ttgcccatct tttcctggct cacttcctcg gcgcggcgca ccgcgtcgtt
     4501 gaaggcggcg gccaccaggt cttcgagcat gtccttgtcg tcggccagca ggctgggatc
     4561 gatggcgacg cgcttgacat cgtgcttgca ggtcatcgtg accttcacga ggccggcgcc
     4621 ggactggcct tcgacctcga tttgcgccag ctcttcctgg gcacgcttga ggttgtcctg
     4681 catcgcctgg gcctgcttca tcaggccggc gagttgtccc ttcatcatgg tgtggtcctc
     4741 gggttcagat gggtttgatc gagccgggca cgatgcgcgc gctcttgtat tgctgcagga
     4801 gcgcgatcac ctgcggatcg ttctggatca gctcctcggc ggcgcgctgg cggcgctcgc
     4861 gctgcgcggc atcgcgttgc gccgggctgt cggtggtggc gccagcctcc agttccagtc
     4921 gcaccggctg cttcaaggcc tgggcgatgg ccgactccag ccgctcgcga tggctgccgc
     4981 cacgcagcgt ctcgcgctcg acgcgcagcc gccagacttc catatcgccc tgtaggccct
     5041 gctgactcac ggccacgcat tgcgcttgca tcgccagctc gcgcaccagc gccgcgatgg
     5101 cgccggcctc gcacagcgag cgcacgagcg ccgaccagcg gtcgccgagt gcgatatcgg
     5161 cgctgctcaa ggcctcgggt tcggcgtcca caggcacggc tgcaatcgcc gcactcgata
     5221 aagcggcacg cgcaggccgg ggcaccacct ctgcggccac gatctccgtc gccatgggag
     5281 catcgaccca gggcgggatc tcagcgtctg tggcagcagc gcttgcggca ctgctgcggc
     5341 tcgcgggggc cggcgcacgc agggcagcgc tgcgagccgc ggcggccga
//
LOCUS       DASUCY010000015         2024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_369835, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000015 DASUCY010000000
VERSION     DASUCY010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369835"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VFV25_00295"
     CDS             <1..608
                     /locus_tag="VFV25_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEY1391782.1"
                     /translation="TAPPLPAPKPKSSTAPAPVSRSTPAAERVQTETTADAGREVGSP
                     AAQTTEDAAAAQAKPEKSDQAADKPSARPSDKKPAVAKPIEKPAADKTASADAAVAKP
                     AAKDGVRAQALLNGGAADEGAAASGRFVVQVGAFSEVTAAREVRHRVEKLGLKTYTQV
                     VETSAGKRIRVRVGPFGERGEAEKAADKLKQAGLNVALLTL"
     gene            617..1105
                     /locus_tag="VFV25_00300"
     CDS             617..1105
                     /locus_tag="VFV25_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEY1391783.1"
                     /translation="MAWVDIALLCILGLSVVLGIARGLVFEVMSLAGWVVAYFAAQWL
                     VPELAPHLPIGSPGSALNAGAAFASGFIAVLIGWGLLCWLIRKLVHASPLSVIDRLLG
                     GFFGLARGLLIGLVAVTLVAYTPLARSADWQASQGVAALQALLAQLKPLLPGEVVIHL
                     PA"
     gene            1150..>2024
                     /locus_tag="VFV25_00305"
     CDS             1150..>2024
                     /locus_tag="VFV25_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEY1391784.1"
                     /translation="MCGIVGVISKAPVNQLIYDALLLLQHRGQDAAGIVTAMGTKFFM
                     HKARGMVRDVFRTRNMRALPGTVGLGQVRYPTAGNAYNEEEAQPFYVNAPFGLVLVHN
                     GNLTNAHALKQELFAIDRRHINTESDSEVLLNVVAHELEVAARDLPLTPEEVFKAMRA
                     VHKRIKGSYAVVCLIAGYGLLAFRDPYGIRPLCIGEADTPEGREVLVASESVALEGTG
                     HKFLRDVAPGEAVFIDLEGRIHSQQCADNASLNPCVFEFVYLARPDSVIDGISVYQAR
                     LNMGETLAQRLISTMP"
BASE COUNT          323 a          664 c          694 g          343 t
ORIGIN      
        1 acacagcgcc gcccttgccc gcgcccaagc ccaagtcttc gaccgcgccg gcgccggtca
       61 gtcgcagcac gcctgcggca gagcgcgtgc agaccgaaac gaccgccgat gcgggccgcg
      121 aagtcggctc gcctgccgcg cagacgaccg aggacgctgc cgctgcgcag gccaagccgg
      181 aaaagtcgga ccaggccgca gacaagccca gcgcgagacc cagcgataag aagcccgcag
      241 ttgcgaaacc catcgagaag ccggcggccg acaagactgc ttccgccgac gccgccgtag
      301 ccaagcccgc ggccaaggac ggtgtgcgcg cgcaggcctt gctgaatggc ggcgctgccg
      361 acgagggtgc agcggccagc gggcgcttcg tggtgcaagt gggcgccttc agcgaagtga
      421 cggccgcgcg cgaggtgcgc catcgcgtcg agaagctggg cctcaagacc tatacgcaag
      481 tcgtcgagac ctcggccggc aagcgcatcc gcgtgcgcgt cggtcctttc ggcgagcgcg
      541 gcgaggccga gaaggccgcc gacaagctca agcaggcagg cctgaatgtc gcgctgctca
      601 cgctctagga aaagcggtgg cctgggtcga catcgcgctg ctgtgtatct tgggcctgtc
      661 cgtggtgctg ggcatcgcgc gcggcctcgt tttcgaggtc atgtccctgg ccggctgggt
      721 ggtcgcctat ttcgctgcgc agtggctggt gccggagttg gcgccccatc tgcccatcgg
      781 ttcgccgggc tcggccttga atgcgggcgc tgcgttcgca tctggcttca ttgcggtgct
      841 gatcggctgg ggcctgctgt gctggttgat ccgcaagctc gtgcatgcct cgccgctgag
      901 cgtgatcgac aggttgctgg gcggcttctt cggcctggcg cgcgggcttc tgatcgggct
      961 ggtggcagtg acgctggtcg cctatacgcc gctggcgaga tcggcggatt ggcaggcctc
     1021 gcagggcgtg gcggctctgc aggccttgct cgcgcagctc aagcccctgc tgccgggcga
     1081 ggtggtcata catctgcccg catgaatgca ggtcttgcgg tacgtttcga tttgatggag
     1141 tgaacaggca tgtgtggcat cgtcggcgtg atttccaagg cgcctgtcaa tcagctgatc
     1201 tatgacgcct tgctgctgct gcagcaccgc ggccaggatg cggcgggcat cgtgaccgcg
     1261 atgggcacca agttcttcat gcacaaggcg cgcggcatgg tgcgcgatgt gttccgcacg
     1321 cgcaatatgc gggcgctgcc cggcacggtc ggcctgggcc aggtgcggta tccgacggcg
     1381 ggcaatgcct acaacgagga agaggcccag cccttctatg tcaacgcacc cttcggcctg
     1441 gtgctggtgc acaacggcaa cctgaccaac gcccatgcgc tgaagcagga gctgttcgcc
     1501 atcgatcgcc gccacatcaa caccgagagc gactccgagg tcctgctcaa cgtggtggcg
     1561 cacgagttgg aggtcgccgc gcgcgacctg ccgctgacgc cggaagaggt cttcaaggcc
     1621 atgcgcgcgg tgcacaagcg catcaagggt tcctatgccg tggtctgcct gatcgcgggc
     1681 tacggcctgc tcgccttccg cgacccctat ggcatccgcc cgctgtgcat aggcgaagcc
     1741 gacactcccg agggccgcga agtgctggtg gccagcgagt cggtggcgct cgaaggcacg
     1801 gggcacaagt tcctgcgcga cgtggcgccc ggcgaagccg tctttatcga tctggaaggc
     1861 cgcatccaca gccagcaatg cgccgacaac gcgagcctca atccctgtgt gttcgagttc
     1921 gtctacctgg cgcgccccga ttcggtgatc gacggcatct cggtctacca ggctcggctc
     1981 aacatgggag agacgctggc ccagcgcctg atctccacca tgcc
//
LOCUS       DASUCY010000016         3934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_546573, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000016 DASUCY010000000
VERSION     DASUCY010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546573"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..172
                     /locus_tag="VFV25_00310"
     CDS             <1..172
                     /locus_tag="VFV25_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY1391785.1"
                     /translation="REHLSVLTGSMEVTAGDATQRLKHGETARYAVDQPHAIRNAGKT
                     VATALLVVLHSS"
     gene            281..1636
                     /locus_tag="VFV25_00315"
     CDS             281..1636
                     /locus_tag="VFV25_00315"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY1391786.1"
                     /translation="MPLDQRVSYIERLVDTIDLPIGRWVGRWLVCSADAHADGPAEQS
                     AASFDGTAAYLSFCNGPYLVWASRSRDELIGRSLAQLYGDTAWAAAREAFGEAFCGRT
                     TYYERQLTHLSGPPRWARIQVFPDIAPDGRVEAVYTIAFDVHDYRMKREALEAARRRL
                     DHFTENIPYPLTYVDRGFVIRFVNRAYCEISGMDAETLVGRHIGEVRGVRRWAEHRPY
                     FEQALSGQAAQYTRLVDLAQGGPRWLRTSYVPDFDVEGEVVGLYTVTIDVHELTVAQE
                     GLRRSVERDAVTDVLSRRTIMDRIDIAMACGADGPIALFFIDLDGFKTVNDRFGHREG
                     DALLMQVAAALQGTMRAEDAVGRFGGDEFLVLAQVRDLAGARVLARHLLQAVQRCTEK
                     LSASHSVTASIGFALAPDDAQNPHKLLQRADDAMYTAKRLGKNRAVHCLGDVVDALGT
                     G"
     gene            complement(1652..2887)
                     /locus_tag="VFV25_00320"
     CDS             complement(1652..2887)
                     /locus_tag="VFV25_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HEY1391787.1"
                     /translation="MNVPASLKQPLRERIAHSLQTLQTWPWLDTLRTLRQRFREDRLG
                     LTASSLTFTTTIALVPLATVTLAIFTAFPMFAQFQVALQKYFIASLVPDSIAKQVLVA
                     LTQFATKANRLGTVGLVALMFTALALMLTIDRTLNAIWRVREPRPIAQRVLVYWAAAT
                     LGPLVLGVSLTLTSYAVSASKGLVGDLPGGVSLLLNSIEFLLLAGGMAGLFRVVPNTH
                     VRWAHALSGGFFVAIGFELAKRGLAWYLGNVPTYTTIYGAFATVPIFLVWLYLGWVIV
                     LLGAVIAAYAPSLQMRVVRRPETPGHRFELAITVLRELAQARSLSTRGLSPAQLSASL
                     RIDPLQIEPLLDTLAALDWAGRLDEAGAARYVLLCDPAATKAQPLIAALLLEPSPPVR
                     GFWHRASLQSLSVQDLIEA"
     gene            2909..3286
                     /locus_tag="VFV25_00325"
     CDS             2909..3286
                     /locus_tag="VFV25_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HEY1391788.1"
                     /translation="MEPPVRIAWTRNIAVGSLLALIALGLAWELWLAPLRPGGSWWAL
                     KVLPLTLPLAGLLKLRMYTYRWLSLLVWLYFTEGVVRATSDPNGRSAALAGLQVLLCL
                     VLFTACALHVRWRLKAGPREAQA"
     gene            3283..>3934
                     /locus_tag="VFV25_00330"
     CDS             3283..>3934
                     /locus_tag="VFV25_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY1391789.1"
                     /translation="MSDLLLQALRGIVGETQVLQGGDLSAYEIDWRKRYRGRALAVVR
                     PADAAEVAAVVRACAAHGASLVPQGGNTGLVGGSVPDASGTQVLLSLARMNRVREIDA
                     ANLTMTAEAGCVLQAAQEAAAARGLLFPLSLAAEGSCTIGGNLATNAGGTQVLRYGNA
                     RELCLGLQVVTAGGEIWQGLSGLRKDNTGYALRELYIGSEGTLGIITAATLKLYPQP"
BASE COUNT          636 a         1326 c         1389 g          583 t
ORIGIN      
        1 gcgcgagcac ctgagcgtgc tcaccggcag catggaggtc acggcgggcg acgcgaccca
       61 gcgcctcaag catggggaaa cggcgcgtta cgcggtcgat cagccgcacg ccatccgcaa
      121 tgccggcaag accgtggcga cggcgctgct tgtcgtgctg catagcagct gagcttggca
      181 gcttcgtggc gcatccccgc aaaggaggtg ccgatgtgtg caatttggct ctggaccagg
      241 cgactcggcg ctgccatgat gccggccacg ggatcctggc atgccactag atcagcgcgt
      301 ctcctacatc gagcgactcg tcgatacgat tgatctgccc atcggtcgct gggtgggtcg
      361 ctggctagtt tgctcggctg atgcgcacgc cgatgggccg gccgagcaat cggccgcctc
      421 cttcgacggc accgccgcct acctgagctt ctgcaacggc ccctacctgg tctgggcgag
      481 ccgcagccgc gatgaattga tcgggcgctc cctggcgcag ctgtatggcg acaccgcttg
      541 ggccgcggca cgcgaggcct tcggcgaggc cttctgcggt cgcaccacct actacgagcg
      601 gcagctcacg cacctgagcg ggcctccgcg ctgggcgcgt atccaggtat tccccgatat
      661 cgcgcccgac gggcgcgtcg aagcggtcta caccatcgcc ttcgacgtgc atgactaccg
      721 catgaagcgc gaggcgctgg aggcggccag gcgcaggctg gaccatttca ccgagaacat
      781 cccctatccg ctgacctacg tcgaccgcgg cttcgtgatc cgcttcgtca accgcgccta
      841 ttgcgagatc agcggcatgg acgccgagac gctcgtgggc cgtcacatcg gcgaggttcg
      901 cggcgtcagg cgctgggccg agcaccgccc ctatttcgag caggccctga gcggccaggc
      961 tgcgcaatac acccggctcg tcgatctcgc ccaggggggc ccgcgctggc tgcgcaccag
     1021 ctatgtgccc gacttcgacg tcgaaggcga agtcgtcggc ctgtacacgg tcaccatcga
     1081 cgtgcatgaa ctcacggtcg cgcaggaggg cctgcgccgc agcgtcgagc gcgacgccgt
     1141 aaccgacgtg ctcagccgcc ggacgatcat ggacaggatc gatatcgcga tggcctgcgg
     1201 cgccgatggg ccgatcgcgc tcttcttcat cgatctcgac ggcttcaaga ccgtcaacga
     1261 ccggttcggg caccgcgaag gcgatgcgct gctgatgcag gtcgccgcgg ccttgcaggg
     1321 cacgatgcgg gccgaggacg cggtcgggcg cttcggcggc gacgagttcc tcgtgctggc
     1381 ccaggtgcgc gacctggccg gagcgcgggt gctggctcgt cacctgctgc aagcggtgca
     1441 gcgctgcacc gagaagctgt cggcctcgca cagcgtgact gcgagcatag gctttgccct
     1501 agcgcctgac gacgcccaga acccccacaa gctgctgcag cgggccgatg atgcgatgta
     1561 taccgcgaag cggctgggca agaaccgcgc cgtgcattgc ctcggcgacg tggtggacgc
     1621 gctaggaacg ggctgagagc gctggctgcc gtcaggcctc gatcaggtcc tgcacgctca
     1681 ggctctggag cgaagcccgg tgccagaagc cgcgcaccgg cggcgagggc tcgagcagca
     1741 gcgcggcaat caggggctgg gccttcgtgg cggcaggatc gcacagcagc acgtagcgcg
     1801 ccgcgccggc ctcgtccagg cggccggccc agtcgagcgc cgccagggtg tcgagcagcg
     1861 gctcgatctg cagcggatcg atgcgcagcg aggccgacag ctgggccggg ctgagccctc
     1921 gggtgctgag cgagcgcgcc tgcgccagtt cgcgcagcac cgtgatcgca agctcgaagc
     1981 gatggccagg cgtctcgggc cggcgtacca cgcgcatctg caggctgggc gcgtaggctg
     2041 cgatcaccgc accgagcagc acgatgaccc agcccaggta gagccagacg aggaagatcg
     2101 gcacggtcgc gaaggcgccg tagatcgtcg tataggtcgg cacgttgccg agataccagg
     2161 ccaggccccg cttggcgagc tcgaagccga tcgcgacgaa gaagccccct gacagcgcat
     2221 gggcccagcg cacatgggta ttgggcacga cacggaacag ccccgccatg ccgccggcca
     2281 gcagcaggaa ctcgatcgag ttgagcagca ggctcacccc gcccggcaag tcgcctacca
     2341 ggcccttcga ggccgagacc gcgtaggagg tcagcgtgag gctcacgccc agcaccagcg
     2401 gcccaagcgt cgccgcggcc cagtagacca gcacgcgctg cgcaatgggg cgcggctcgc
     2461 gcacgcgcca gatcgcgttg agcgtgcggt ctatcgtgag catcagcgcc agggccgtga
     2521 acatcagggc caccaggccg accgtgccga gccgattggc cttggtggcg aactgggtca
     2581 gcgccaccag cacctgcttg gcgatgctgt ccggcaccag gctcgcgatg aagtacttct
     2641 gcagcgccac ctggaactgc gcaaacatcg gaaaggccgt gaagatggcg agcgtgaccg
     2701 tcgccagcgg caccagcgcg atggtggtcg tgaaggtcag gctgctggcc gtgaggccga
     2761 gcctgtcctc gcgaaagcgc tggcgcagcg tcctgagcgt gtcgagccag ggccaggtct
     2821 gcagggtctg cagcgagtgc gcgatgcgct cgcgcaaggg ctgcttcaag gaggcgggga
     2881 cattcatggg gcccgtatca tgccagccat ggagccaccc gttcgcattg cctggacacg
     2941 aaacatcgcc gtcggcagcc tgctcgccct gatcgcgctc ggcctggcct gggagttgtg
     3001 gctggcgccg ctgcggccgg gcggctcgtg gtgggccctg aaggtgctgc cgctgaccct
     3061 gccgctggcc gggctgctga agctgcgcat gtacacctac cgctggctga gcctgctggt
     3121 ctggctctat ttcaccgagg gcgtcgtacg cgccaccagc gatcccaacg gccgcagcgc
     3181 cgcgctggcg ggcctgcaag tactgctgtg cctcgtactg ttcacagcct gcgcgctgca
     3241 tgtgcgctgg cgacttaagg ccgggccgcg ggaagcgcag gcatgagcga tttgttgctg
     3301 caggccctgc gcggcatcgt gggcgagacc caggtgctgc agggcggcga tctcagcgcc
     3361 tacgagatcg actggcgcaa gcgctatcgc ggcagggccc tggcggtggt gcgcccggcc
     3421 gatgcagctg aagtggccgc cgtcgtgcgg gcgtgcgcgg cgcatggcgc gagcctcgtg
     3481 ccccagggcg gcaataccgg cctggttggc ggctcggtgc cggatgcgag cggcacgcag
     3541 gtgctgctca gcctcgcacg catgaaccgg gtgcgcgaga tcgacgccgc caatctgacg
     3601 atgaccgccg aggccggttg cgtgctgcag gccgcgcagg aggcggccgc ggcgcggggc
     3661 ctgctgttcc cgctcagcct cgcggccgaa ggcagctgca cgataggcgg caacctcgcc
     3721 accaatgcgg gcggcaccca ggtgctgcgc tacggcaatg cgcgcgagct ctgcctcggg
     3781 ctgcaggtcg tgacggccgg cggcgagatc tggcagggct taagcgggct gcgcaaggac
     3841 aacacaggct atgcgctgcg cgagctctac atcggcagcg aaggtacgct gggcatcatc
     3901 acggccgcca cgctcaagct ctacccccaa ccgg
//
LOCUS       DASUCY010000017         2321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1028482, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000017 DASUCY010000000
VERSION     DASUCY010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028482"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VFV25_00335"
     CDS             complement(<1..554)
                     /locus_tag="VFV25_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1391790.1"
                     /translation="MDLQAIRTHWQRRPWLWGGLAALLALALLAFVGFRIAVNELRSQ
                     LTQALGPRSEIGELGVGWAGVEARQVRIRAATHGWPTGEELRAQRIIVTPSLAGFFSP
                     GWHIARIRIEDGYLSLLRGRDGRLHVLPALLEREEAGIAARKAAAGESAPRIFIGDVE
                     LDGAVIEFYDATVRQPPLRVRLQEL"
     gene            585..1421
                     /locus_tag="VFV25_00340"
     CDS             585..1421
                     /locus_tag="VFV25_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HEY1391791.1"
                     /translation="MPAIDISAPLRLAGVDFTSAPTRRKPITVALGDLSTGIVRLARV
                     DAHVDFASFSAWLRAPGPSLAAFDFPFGLPRELVTALDWPTEWLPLMQHYTRLSRTQI
                     REAFAAFCAARPAGAKFAHRATDGPAGSSPSMKWVNPPVAYMLHAGVTMLIEAGLHLP
                     GLHAGDPQRVGLEGYPGLLARELIGTRSYKSDTRAKQTPERLLARKDLIEALEQGRTR
                     LGLRLKLSHAQRDALAADAAGDRLDAVLCLMQAAWASHQPRHGLPPQVDPIEGWIVSA
                     PW"
     gene            1472..>2321
                     /locus_tag="VFV25_00345"
     CDS             1472..>2321
                     /locus_tag="VFV25_00345"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEY1391792.1"
                     /translation="MKPWPKFVSRLPVLLCALALAGVAQAQFEPGKVKPEPPGVAAHF
                     AEPAVRYATPGLREAREDFTSHAEMLAFVESLAKAAPRLLHVEVLGQSQEGRAMPLLV
                     LSAKSGVNPKLPTVLVLGQQHGNEPAGGEAALVLAQQLSGPRASLLEQVNVLIVPRAN
                     PDGAERFTRVSANGIDINRDHLLLRTPEARLIAGVAARYRPQVVLDLHEFTVGDRWIA
                     KFGAYERYDALLQAATVGNLNPRLGEFSEHAFLDTMRAALAADGQHAFWYHTSSTSGS
                     DKRVSMG"
BASE COUNT          353 a          807 c          798 g          363 t
ORIGIN      
        1 agctcctgca gtcgcacgcg caatggcggc tggcgcaccg tggcgtcgta gaactcgatc
       61 actgcgccat ccagctcgac gtcgccgatg aagatgcgcg gcgcactttc gccggccgca
      121 gccttgcgcg cggcaatgcc cgcctcctcg cgttcgagca gcgcgggaag cacatgcagg
      181 cggccgtcgc ggccgcgtag caggctcaga tagccgtcct cgatgcgtat gcgcgcaata
      241 tgccagccgg gggagaagaa gccggcgagc gaaggcgtca cgatgatgcg ctgggcgcgc
      301 aattcctcgc cggtgggcca gccgtgcgtg gcggcgcgta tgcgcacctg gcgcgcctcc
      361 acgccggccc agccgacgcc gagctcgccg atctcgctgc gcgggccgag cgcctgcgtg
      421 agctgggagc gcagttcatt caccgcgatg cgaaagccca cgaaagccag cagagccagg
      481 gcgagcagag ccgcaaggcc gccccatagc cagggtctgc gctgccagtg agtgcgtatc
      541 gcttgcaagt ccatggcgag gattgtcgca agtacattgc ggacatgccc gccatcgaca
      601 ttagcgcgcc gctgcgtctg gccggcgtgg atttcaccag cgcgccgacg cggcgcaagc
      661 cgatcacggt ggcgctgggg gacttgagca cgggcatcgt caggctcgcg cgggtcgatg
      721 cccacgtcga ctttgcaagc ttctcggcct ggctgcgcgc gccagggccc agtcttgcgg
      781 ccttcgactt tcccttcggc ctgcctcgtg agctggtgac tgcgctggac tggcccactg
      841 aatggctgcc gctgatgcag cactacacgc gcctcagccg cacgcagata cgcgaagcgt
      901 tcgcggcttt ctgcgcggcg cggccggccg gagccaagtt cgctcatcgc gctaccgacg
      961 gtccggccgg ctcctcgccg tcgatgaaat gggtcaaccc gccggtggcc tacatgctgc
     1021 atgccggcgt gactatgctg atcgaggccg gcctgcacct gcccggcctg catgcgggcg
     1081 atccgcagcg cgtcggtctg gaaggctatc cggggctgct ggcgcgcgag ctgatcggca
     1141 cgcgttccta caagagcgac acgcgcgcca agcagacgcc cgagcgtctg ctggcgcgca
     1201 aggatctgat cgaagcgcta gagcagggcc gcacgcgctt gggcctgcgt ctgaagctca
     1261 gccatgcaca acgcgacgcg ctggcggccg atgctgccgg cgaccggctg gacgccgtgc
     1321 tctgcctgat gcaggctgcc tgggcctcgc accagccgcg ccacggcctg ccgccgcaag
     1381 tcgacccgat cgagggttgg atcgtcagcg caccctggtg agggccggct gtgccagact
     1441 gcggcctgcc cctttcgagg agaaactccc aatgaagcct tggccgaagt ttgtgtcgcg
     1501 tttgccggtg ctgctgtgtg cattggcgct cgccggcgtc gctcaggccc agttcgagcc
     1561 aggcaaggtc aagcccgagc cgccgggcgt ggccgcgcat ttcgccgagc ccgcagtgcg
     1621 ttatgccacg ccgggcctgc gcgaagcccg cgaagacttc accagccatg ccgagatgct
     1681 ggccttcgtc gagtcgctgg ccaaggccgc gccgcggctg cttcatgtcg aagtgctggg
     1741 ccagtcgcag gaggggcggg ccatgccgct gctggtgctc agcgccaaga gcggcgtcaa
     1801 ccccaagctg ccgacggtgc tcgtgctcgg gcagcagcat ggcaacgagc cggccggcgg
     1861 cgaggcggct ctggtgctgg cccagcagct gagcggcccg cgcgccagcc tgctcgagca
     1921 ggtgaacgtc ctcatcgtgc cgcgcgccaa tcccgacggc gcggagcgct tcacgcgggt
     1981 ttcggccaac ggcatcgaca tcaaccgcga ccacctgctg ctgcgcacgc ccgaagcgcg
     2041 gctgattgcc ggcgtcgccg ctcgctaccg gccccaggtg gtgctggact tgcatgagtt
     2101 caccgttggc gaccgctgga tcgcgaagtt cggtgcctac gagcgctacg acgccctgct
     2161 gcaggccgct acggtcggca atctgaatcc caggctgggt gaattctcgg aacatgcctt
     2221 cttggatacc atgcgcgccg cgctggcggc cgacggccag cacgcgttct ggtaccacac
     2281 cagctcgacc agcggcagcg acaagcgcgt ctccatgggc g
//
LOCUS       DASUCY010000018         3398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_755430, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000018 DASUCY010000000
VERSION     DASUCY010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755430"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1250
                     /locus_tag="VFV25_00350"
     CDS             <1..1250
                     /locus_tag="VFV25_00350"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY1391793.1"
                     /translation="DDVLWSLNRINDAHHPAHAAARNGFPYWAGMSMPGLIQSIARLD
                     DYTVRITLTHPEAPFLADLAMSALASVYSAEYGEQLTRAGRLEELNTQPIGSGPYAFK
                     SYQKDAVIRYVAHNAYWGGKPALDQLVFAITPDPSVRAQRLKAGECAVANVIGEQAES
                     LASDSRIAVVPNPQLTTSYVAPNAKRRFLSDKRFRLALSLAIDRASLVKSVYGGYATV
                     ATSFVPPGIWSHDASLANPQDIEKAKALVKASGYDGSELTLFSVARRSDNKRAVELLQ
                     ADWARIGVKVRPQLMELGELYKRTGKGEHDLAFLSWFSDNGDPDNFLSPNLACAAVEG
                     GGNKAQWCNKTLDALLARARATTDMKQRTQLYLQAQKLLHDETGLIPIANKQGLSAVR
                     KDVKGYQATLFGGNDFRAARIGD"
     gene            1266..2294
                     /locus_tag="VFV25_00355"
     CDS             1266..2294
                     /locus_tag="VFV25_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY1391794.1"
                     /translation="MFGFLLRRLALVVPTFLGITLLAFSLIHLIPGDPVQVMAGERRL
                     DPAFYAETLHRLGLDRPMWEQYASYLWKALHLDLGQSFVTQEAVWSEFTKLFPATLEL
                     SVCALLFATVFGLLAGVLAAIKRGTVLDHGVMGLSLTGFSMPIFWWGLLLIMFFSVYM
                     RDWLPALALPVSGRMALEFDIAPRTGFMLIDAWLSGEPGAFRSAVSHLVLPAIVLGTV
                     PLAVIARMTRSAMLEVLREDYVRTARAKGLSPARVVFVHALRNALVPVVTVIGLQVGT
                     LLGGAVLTETIFSWPGIGKWLIDAISRRDYPVVQGGVLLVATLVIVVNLLVDLLYGLV
                     NPRIRHAK"
     gene            2299..3210
                     /locus_tag="VFV25_00360"
     CDS             2299..3210
                     /locus_tag="VFV25_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY1391795.1"
                     /translation="MDAALATEEAGHAGAPPSSLQDFWRSFAHNKGAVAGLIFFALVV
                     LVALLAGVLAPHDPIEQFRDALLTPPMWSTDGSSRFILGTDDIGRDILSRLLHGARLS
                     LLIGLVAVLLSMLPGIALGLLAAFFPSKLGTLILRLMDVLLALPSLLLAIAVVAVLGP
                     GLLNTMCAIAIVAMPGYVRLVRASAMAELTKDYVVASRLAGAGTLRLMFDTVLPNCMA
                     PVIVTATLCFSDAILTAAALGFLGLGAQPPTPEWGAMLASARDYIERANWVVTLPGVA
                     ILSTVLAINLMGDGLRDALDPRMKKLS"
     gene            3207..>3398
                     /locus_tag="VFV25_00365"
     CDS             3207..>3398
                     /locus_tag="VFV25_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014750123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY1391796.1"
                     /translation="MSLLEIRNLTVEFGPKERPFAVVQGVDLTLERGELLGVVGESGS
                     GKSVSMLALMGLIDAPGRVS"
BASE COUNT          497 a         1192 c         1121 g          588 t
ORIGIN      
        1 ccgacgacgt gctgtggtcg ctcaaccgca tcaacgatgc gcaccacccg gcgcatgcgg
       61 cggcgcgcaa cggctttccc tactgggccg gcatgagcat gcccggcctg atccagagca
      121 tcgccaggct ggacgactac accgttcgca tcacgctgac gcacccggag gcgcccttcc
      181 ttgccgacct cgcgatgtcg gcgctggcct ccgtctattc ggccgaatac ggcgagcagc
      241 tgacgcgcgc cggtcggctg gaagagctga acacccagcc gatcggcagc gggccttacg
      301 ccttcaagag ctatcagaag gacgcggtaa tccgctacgt ggcccacaac gcctactggg
      361 gcggcaagcc cgcgctggac cagctggtgt tcgcgatcac gcccgacccc agcgtgcggg
      421 cgcagcgctt gaaggccggt gaatgcgcgg tcgccaacgt catcggcgag caggccgaga
      481 gcttggcgag cgacagccgc atcgcggtcg tccccaatcc gcaactgacg acgagctatg
      541 tcgcgcccaa tgcgaagcgc cgcttcctga gcgacaagcg cttccggctc gcgctgtcgc
      601 tggccatcga ccgcgccagc ctcgtgaaat cggtctatgg cggctatgcc accgtggcga
      661 ccagcttcgt gccgcccggc atctggagcc acgacgccag cctcgccaac ccgcaggaca
      721 tcgagaaggc caaggccctg gtcaaggcct cgggctacga cggcagcgag ctcacgctgt
      781 tctcggtcgc gcgacgcagc gacaacaagc gcgccgtgga gttgctgcag gccgactggg
      841 cccgcatcgg cgtcaaggtc aggccacagc tgatggagct gggcgagctc tacaagcgca
      901 ccggcaaggg cgagcatgac ctcgccttcc tgagctggtt cagcgacaac ggcgacccgg
      961 acaacttcct gagccccaac ctcgcctgtg cagccgtcga gggcggcggc aacaaggccc
     1021 agtggtgcaa caagaccctg gacgccctgc tcgcccgggc tcgcgccacg accgacatga
     1081 agcagcgcac ccagctctac ctgcaggcac agaaactgct ccacgacgaa accggcctga
     1141 tcccgatcgc caacaagcag ggcctgagcg ccgtgcgcaa ggacgtcaag ggctaccagg
     1201 ccaccttgtt cggcggcaac gatttccgcg ccgcgcgtat tggcgactga ttggcgactg
     1261 atctgatgtt tggcttcctg ctgcgccgcc tggcgctcgt cgttcccacc ttcctcggca
     1321 tcacgctgct ggccttctcg ctgatccacc tgatccccgg cgatccggtg caggtgatgg
     1381 ccggcgagcg ccgcctggac ccggccttct atgccgagac cctgcatcga ctggggctgg
     1441 atcggcccat gtgggagcaa tacgcgagct atctgtggaa ggcgctgcac ctggatctgg
     1501 gccagtcctt cgtgacccag gaggcggtgt ggagcgagtt caccaagctc ttcccggcca
     1561 cgctcgagct ctcggtctgc gcgctgctct ttgccaccgt cttcggcctg ctggccggcg
     1621 tgctggcggc catcaagcgc ggcaccgtgc tcgaccacgg cgtgatgggc ctgtcgctga
     1681 cgggcttttc gatgcccatc ttctggtggg gcctgctgct catcatgttc ttctcggtct
     1741 acatgcgcga ctggctgccg gcgctggcgc tgccggtgtc gggccgcatg gcgctggaat
     1801 tcgacatcgc cccgcgcacc ggcttcatgc tgatcgacgc ctggctgtcg ggcgagcccg
     1861 gcgccttccg ctcggctgtc tctcacctcg tgctgccggc catcgtgctc ggcaccgtgc
     1921 cgctggcggt aatcgcgcgt atgacgcgct cggccatgct ggaggtgctg cgcgaggact
     1981 atgtgcgcac cgcccgtgcc aagggcctgt cgccggcgcg cgtggtcttc gtgcatgcgc
     2041 tgcgcaatgc gctggtgccg gtcgtgaccg tgatcggcct gcaggtcggc accctgctcg
     2101 gcggcgccgt gctgaccgag accatcttct cctggcccgg catcggcaag tggctgatcg
     2161 atgcgatcag ccggcgcgac taccccgtcg tgcagggcgg cgtgctgctc gtcgcgacgc
     2221 tggtgatcgt cgtcaacctg ctggtcgacc tgctgtacgg actggtcaac ccgcgaatcc
     2281 gccacgccaa ataaagcaat ggacgcagcc ctcgccaccg aagaagccgg ccacgccggc
     2341 gccccgccct cctcgctgca ggacttctgg cgcagctttg cgcacaacaa gggcgcggtc
     2401 gcgggcctga tcttttttgc gctggtcgtg ctggtcgcgc tgctggccgg cgtgctggcg
     2461 ccgcacgatc cgatcgagca gttccgcgac gcgctgctga cgccgccgat gtggagcacg
     2521 gacggcagca gccgcttcat cctgggcacc gacgacatcg gccgcgacat cctgtcgcgc
     2581 ctgctccacg gcgcgcggct gtcgctgctg atcggcctgg tcgcggtgct gctgtccatg
     2641 ctgcccggca tcgcgctcgg cctcttggcc gccttctttc cgagcaagct gggcacgctg
     2701 atcctgcgcc tgatggacgt gctgctggcc ctgccgagcc tgctgctggc gatcgccgtc
     2761 gtcgcggtgc tcgggcccgg cctcctcaac acgatgtgcg cgatcgccat cgtcgcgatg
     2821 ccgggctatg tgcggctggt gcgcgcctcg gcaatggccg agctgaccaa ggactacgtg
     2881 gtcgcctcgc ggctggccgg cgccggcacc ttgcggctga tgttcgatac cgtgctgccc
     2941 aactgcatgg cgccggtgat cgtgaccgcg acgctgtgct tctcggacgc catcctcacc
     3001 gcggcggccc tgggtttcct gggcctgggc gcccagccgc cgacgccgga atggggtgcg
     3061 atgctggcct cggcccgcga ctacatcgag cgcgccaact gggtcgtcac gctgcccggc
     3121 gtcgccatcc tgtccaccgt gctcgcgatc aatctgatgg gcgacgggct gcgcgatgcg
     3181 ctggatccgc gcatgaagaa gttgtcatga gtttgctgga gatcagaaac ctcacggtcg
     3241 aattcgggcc gaaggaacgc cccttcgcgg tcgtgcaagg cgtcgacctg acgctggagc
     3301 gcggcgagct gctgggcgtg gtcggcgaat cgggttcggg caagagcgtc agcatgctcg
     3361 cgctgatggg cctgatcgac gcacccgggc gagtcagc
//
LOCUS       DASUCY010000019         2281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1124942, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000019 DASUCY010000000
VERSION     DASUCY010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124942"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VFV25_00370"
     CDS             <1..327
                     /locus_tag="VFV25_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1391797.1"
                     /translation="WVLHMRSEVVISFGPGQEISQALDGLTTMSPEAARAWLDEQFVK
                     LGCEPLRPTGKVLIADKLVAIAAAVGHARFVSDVAWARDYVRAASAALGKSAVRVDVP
                     GLTIAY"
     gene            506..892
                     /locus_tag="VFV25_00375"
     CDS             506..892
                     /locus_tag="VFV25_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HEY1391798.1"
                     /translation="MMMTTQTRSLHPAPGRDLSMLRVARKLILLSAASFALGAQAQTP
                     SATRAQLPSKELLAAGQEVTPRDLAAAQAAFTRADTNKDGKLSREEVAAGLPALSPKF
                     DELDKNHDGYLSFEEFAAGFMPSDKH"
     gene            complement(970..1554)
                     /locus_tag="VFV25_00380"
     CDS             complement(970..1554)
                     /locus_tag="VFV25_00380"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEY1391799.1"
                     /translation="MANRLSQIATRTGDDGTTGLGDGSRVPKDHLRVQAMGEVDELNS
                     QLGLLLCEPLPDDVRELLVVIQHELFNLGGELSIPGYELLKEEAVARLDQALAHYNAT
                     LPRLKEFILPAGTRSAALAHVGRTVARRAERALVALAAQESVRNAPRQYLNRLSDLLF
                     VLARVLNRANLDGLGGDDVYWRSERLARQGSAED"
     gene            1632..>2281
                     /locus_tag="VFV25_00385"
     CDS             1632..>2281
                     /locus_tag="VFV25_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEY1391800.1"
                     /translation="MNAPQELRLAPRPMPQAMLSALKARFAQRCSTAQAVREQHGRDE
                     SPFDAPPPDAVVYCESNEDVAFVVALASEHEVPVIPYGAGSSLEGHLLAVQGGVSIDL
                     SRMNAILRVNAEDLTVTVQAGVTRKQLNEAVKHEGLFFPIDPGADASIGGMCATRASG
                     TNAVRYGTMRENVLALEVVTASGEIIRTGTRAKKSSAGYDLTRLFVGSEGTLGVIT"
BASE COUNT          372 a          771 c          763 g          375 t
ORIGIN      
        1 tgggtactgc atatgcgcag cgaagtcgtc atcagcttcg gcccggggca ggagatcagc
       61 caggcgctgg acggcctgac caccatgtcg cccgaggccg ctcgcgcatg gctggacgag
      121 caattcgtca agctcggctg cgagccgctg cgccccaccg gcaaggtatt gatcgccgac
      181 aagctcgtcg ccatcgcggc cgcggtaggc catgccaggt tcgtcagcga tgtggcttgg
      241 gcgcgcgact acgtccgcgc cgcgagcgcc gccctcggca agtcggcggt gcgcgttgac
      301 gtgcccgggc tcacgatcgc ctactgaagc tcgcagcgga aggaagttcc gcctgcaacc
      361 cccaacgtaa gtgtgcactt gcaatagcaa gtaagcccat tccttcagtc tgtaagccgg
      421 gcaagcttgg gcaaaagcgc tggccgcacc ggcggcgtcg gcgtgcaatg gggcctcctt
      481 caactctctc caaggaaggc cttcgatgat gatgacgacg cagactcgtt ccttgcatcc
      541 ggcgcccggc cgcgacctca gcatgctgcg cgtggcgcgc aagctgatcc tgctgagcgc
      601 ggccagcttc gcgctgggcg cccaggccca gacgccatcg gccaccaggg ctcaattgcc
      661 gagcaaggag ttgctggccg ccggccagga agtcacgccg cgcgatctgg ccgcggccca
      721 ggccgccttc acgcgggccg ataccaacaa ggacggcaag ctttcgcgcg aggaagtggc
      781 ggccggtctg cctgccctgt cgcccaagtt cgacgagctg gacaagaacc acgacggcta
      841 cctgtctttc gaggaattcg ccgccggctt catgcccagc gacaagcact agtcctcctc
      901 cctttcctcc tctctcgacg ctgaccgcgt cgagtgtttc cagcccgccg cgcgcgggct
      961 tctttttgct cagtcctcgg ccgacccctg cctggccagc ctttcgctgc gccaatagac
     1021 atcgtcgccg cccaggccgt cgaggttggc gcggttgagc acgcgggcca gcacgaagag
     1081 caggtccgag aggcgattga ggtattggcg cggcgcattg cgcacgcttt cctgcgcggc
     1141 cagcgccacc agggcacgct cggcgcggcg cgccacggtg cggcctacgt gggccagcgc
     1201 ggcactgcgc gtgccggccg gcaggatgaa ttccttgagc cgcggcagcg tcgcgttgta
     1261 atgggccagc gcttggtcca gccgcgcgac ggcctcttcc ttcagcaact cgtagccggg
     1321 gatggacagc tcgccgccga ggttgaagag ctcatgctgg atcacgacca gcagttcgcg
     1381 cacatcatcg ggcagcggct cgcacagcag caggccgagc tgggaattga gctcgtccac
     1441 ctcgcccatg gcctgcacgc gcaagtggtc cttgggcacg cggctgccat cgcccaggcc
     1501 ggtggtgccg tcgtcgccgg tgcgggtggc gatttgcgag agtcgattgg ccatgatgct
     1561 caagcggaag tgcggatgcg gcagtgtagg ccgctgccta gaatcgagtt ccctctggag
     1621 tcttcgtgat catgaacgcg ccccaggaac tgcgcctcgc gccccgcccc atgccgcaag
     1681 cgatgctgtc ggcgctcaag gccagatttg cgcagcgctg ttccaccgcg caggccgtgc
     1741 gcgagcagca tgggcgcgac gagtcgccct tcgatgcgcc gccgcccgat gcagtcgtgt
     1801 attgcgagag caatgaggac gtggccttcg tcgtcgcgct ggcgagcgag cacgaagtgc
     1861 cggtgatccc ttatggcgcg ggctcgtcgc tagagggtca tctgctcgcg gtgcaagggg
     1921 gcgtgagcat cgatctgtcg cgcatgaacg cgatactgcg cgtcaacgcg gaggatttga
     1981 ccgtgaccgt gcaggccggc gtgacgcgca agcagctcaa cgaggcggtc aagcacgaag
     2041 ggctgttctt ccccatcgac cccggtgccg atgcgtcgat cggcggcatg tgcgcgacgc
     2101 gcgccagcgg cacgaacgcg gtgcgctacg gcacgatgcg cgagaacgtg ctggcgctcg
     2161 aagtggtgac cgccagcggc gagatcatcc gcaccggcac gcgcgccaag aagtcttccg
     2221 ccggctacga cctcacccgc ctcttcgtcg gcagcgaagg cacgctgggc gtgatcaccg
     2281 a
//
LOCUS       DASUCY010000020         2765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_418028, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000020 DASUCY010000000
VERSION     DASUCY010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418028"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1487)
                     /locus_tag="VFV25_00390"
     CDS             complement(<1..1487)
                     /locus_tag="VFV25_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY1391801.1"
                     /translation="MHNKMTTLAGALALAFASSASAQTQTTTPPPVTQDAQPAPAAPA
                     PAASDAASAAAAGKAPMAATELDRVDIVEQRQRLDAARNSLSPETGSTVYRFEQQDIK
                     NLPLGDATPLNQVALQAPGVVQDSFGQLHVRGDHGNLQYRVNGVVIPEAISGFGQALD
                     TRFADRITLITGALPAQYGYRTAGVIDINTKGANLENGGSVDAVAGSRGHREGSASIG
                     GTSGALSYFFSGSYLENDIGIENPTPESSALHDKTRQAKGFGYLSYLLGKDSRISFIT
                     ATTDNRFQIPDRPGLTPSFTLANATPPASESLDAKQRERNKFQVLSFQSSLGAGLDYQ
                     VSLSHRDTDVHYRPDSVGDLVYNGVAAEILRSNEAYGLQADLSKKLNDQHTLRFGLFA
                     QRERGKVDNSSLVFPADANGNQTSTTPETIVDDNRLGGHLYGVYLQDEWKPTPALTVN
                     YGARYDQVDTVVKESQLSPRLGATWQLGENTRLHAGYARYFTPPA"
     gene            complement(1657..1986)
                     /locus_tag="VFV25_00395"
     CDS             complement(1657..1986)
                     /locus_tag="VFV25_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1391802.1"
                     /translation="MSSSPASHLRALLARTCLAVMLLFAQQHATLHWLSHAIEATNKA
                     GHVPPPGDHCDQCAGLTAFGAAMTSLGVSLPASLGHEALNAAPQARAVALAPRYAYLS
                     RAPPVLS"
     gene            2104..2673
                     /locus_tag="VFV25_00400"
     CDS             2104..2673
                     /locus_tag="VFV25_00400"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEY1391803.1"
                     /translation="MRSPVELSKAYRLLNHGPTVLVSSAHAGRRNVMAAAWSMPLDFD
                     PPKVAVVIDKSTYTRELIEASGSFALNVPTRALLDATNAVGNVSGRDEDKFSRIGLNG
                     FAAERIAAPLIEGCVGWLECRMLPEPEMARKYDLFLGEVIAAWADERVFSRGRWHFDE
                     QDDDLRSIHHVAGGAFFATGEAVKAQGYR"
BASE COUNT          450 a          873 c          975 g          467 t
ORIGIN      
        1 ctggcgggcg gcgtgaaata gcgcgcatag ccggcgtgca gccgcgtgtt ctcgccgagc
       61 tgccaggtcg cgcccagtcg cgggctcagc tgcgactcct tcaccaccgt gtcgacctgg
      121 tcgtagcgcg cgccgtagtt cacggtcagc gccggcgtgg gcttccattc gtcctgcaga
      181 tagacgccgt acaggtgccc gcccaggcgg ttgtcgtcga caatggtctc gggcgtggtg
      241 ctggtctggt tgccgttggc atcggccggg aagaccagcg agctgttgtc caccttgccg
      301 cgctcgcgtt gggcgaacag gccgaagcgc agcgtgtgct ggtcgttgag cttcttgctc
      361 agatcggcct gcaggccata ggcctcgttg ctgcgcagga tctcggctgc cacgccgttg
      421 tagaccaggt cgccgacgct gtcggggcgg taatgcacgt cggtgtcgcg atgcgagagc
      481 gagacctgat agtccagccc agctcccagg ctggattgga agctcagcac ctggaacttg
      541 ttgcgctcgc gctgcttggc gtccaggctc tcggatgccg gcggcgtcgc gttggccagc
      601 gtgaagctgg gcgtgagccc gggccggtcc gggatctgga agcgattgtc ggtggtggcg
      661 gtgatgaagc tgatgcggct gtccttgccc agcagatagg acagatagcc gaagcccttg
      721 gcctgtctcg tcttgtcgtg cagagcagag ctctcgggcg tcgggttctc gatgccgatg
      781 tcgttttcca gataagagcc gctgaagaaa tacgacagcg cgccgctcgt gccgccgatc
      841 gacgcgctgc cttcgcgatg gccgcggctg ccggcaaccg catcgacact gccgccgttc
      901 tcgaggttgg cgcccttggt gttgatgtcg atcacgccgg cggtgcggta gccgtattgc
      961 gccggcaagg cgccggtgat cagcgtgatg cgatcggcaa agcgcgtgtc cagcgcctgg
     1021 ccgaagccac tgatcgcttc ggggatcacg acgccgttga cgcggtactg cagattgccg
     1081 tggtcgcctc gcacatgcag ctggccgaag gaatcctgca ccacgccggg ggcctgcagc
     1141 gcgacctgat tcagtggcgt tgcatcgccc aagggcaggt tcttgatgtc ctgctgctcg
     1201 aagcggtaga ccgtgctgcc ggtctcgggc gacagcgaat tgcgcgccgc gtccaggcgc
     1261 tggcgctgct cgacgatgtc gacgcgatcg agctcggtcg cagccatggg agccttgccg
     1321 gcggcggcag ccgatgcagc gtcggaagcg gcgggcgccg gagccgcagg agcgggttgc
     1381 gcatcctgcg tcacgggcgg cggcgtagtg gtctgggtct gggcagaagc ggacgaagcg
     1441 aaggcgagcg caagggcgcc ggccagggtg gtcatcttgt tgtgcatgtc tttcccttcc
     1501 ggatgcaaac cctggtcggc caggtgcccg caaactgagc aaagctgtcg gtcaaatgca
     1561 gcacgcgagc cggtcacacc ggctgtgcga gagtcgaggc gaagcggaaa cggcgcagcc
     1621 gcgtggagag gcaccagggc ctcggcagcg ctgcgctcag ctcaggacgg gaggggcgcg
     1681 ggagagatag gcgtagcgcg gtgcgagcgc gactgctctc gcttgcggcg ccgcgttcag
     1741 agcctcgtga ccaaggctcg ccggcagcga aacgccgagg ctggtcatgg cggcgccgaa
     1801 ggcggtcagg cccgcgcatt ggtcgcaatg gtcgccgggc ggcggcacat ggccggcctt
     1861 gttcgtcgcc tcgatcgcgt gcgagagcca gtgcagcgtg gcgtgctgct gagcgaacag
     1921 cagcatcacc gccaggcagg tcctggccag cagtgcacgc agatgggagg cgggggacga
     1981 cgacatcggg gcaagaactc gaagcctgct cggagctagc ccactggcag aagttccgat
     2041 tcggtcgagg ccggcattct gcctcagcct gtaagaaagc tttgtgaccc taaacttcgc
     2101 cccatgcgca gccccgtcga actctccaag gcctatcgcc tgctcaacca cggtcccacc
     2161 gtgctcgtca gctccgcaca tgcggggcgc cgcaacgtga tggcagcggc atggtccatg
     2221 ccgctggatt tcgatccgcc gaaggttgcg gtggtgatcg acaagagcac ctacacccgc
     2281 gagctgatcg aggcctcggg cagcttcgcg ctcaacgtgc cgacgcgcgc attgctcgac
     2341 gcgaccaatg ccgtgggcaa cgtcagcggc cgcgacgaag acaagttctc ccgcatcggg
     2401 ttgaacggct tcgcggccga acgcatcgcc gcgccgctga tcgagggctg cgtcggctgg
     2461 ctcgaatgcc gcatgctgcc cgagcccgag atggcgcgca agtacgacct cttcctcggc
     2521 gaagtgatcg ccgcctgggc cgatgagcgc gtgttcagcc gcggccgctg gcatttcgac
     2581 gagcaggatg acgacctgcg atccatccac catgtcgcag gcggcgcctt cttcgcaacc
     2641 ggcgaggccg taaaagcgca aggctaccga tgagccatcc ggcgcggcaa aaggcaacgg
     2701 ctggcggcaa gagcgggcaa gccgcttcca ccgctgagcc gatcatcaag gccttcaagc
     2761 accag
//
LOCUS       DASUCY010000021         2289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_401961, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000021 DASUCY010000000
VERSION     DASUCY010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401961"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..1837
                     /gene="kdpA"
                     /locus_tag="VFV25_00405"
     CDS             41..1837
                     /gene="kdpA"
                     /locus_tag="VFV25_00405"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEY1391804.1"
                     /translation="MSTHAWLLLGVYSLVLLALSLPMGRYLAYVMEGRFSFGQRIERV
                     LYRITGVQADEEMGWLRYAIGLLLFNGLGVLAVYALQRLQAWLPLDPQQFSNVSADSS
                     FNTAVSFVTNTDWQGYTPEQTMSHLTQMLGLAVQNFLSAATGIVVVIALIRGFARKSA
                     ETIGNLWVDLARITLWVLLPICTIYAVFLVSQGSVQSFKGFTEATTVEVTKYQEPVLG
                     KDGQPVKDAKGNPVMKDAQTDKQQLPMGPVASQEAIKMLGTNGGGFFNANSAHPYENP
                     TALTNFVQMLSIFVIPGGLCFAFGIMVVDRRQGWTVYVAMFLLFVAGAIAATHFEQQG
                     NPLLAKLGVDQTLSATQPGGNMEGKETRFGIADSALFATITTDASCGAVNSMHDSFLP
                     LGGFVPLVNMQLGEVVFGGVGSGLYGMLVFAIVAVFVAGLMIGRTPEYLGKKIETYEM
                     KMTSIAVLVTPLLVLALAAVAVMSEGGKAGIANPGAHGFSEILYAFTSVANNNGSAFA
                     GLSANTPFYNIVLAVGMWFGRFGVIVPVLALAGSLAAKKRITAGAGTLPTHGPLFIAL
                     LIGTVLLVGLLNYVPALALGPVVEHFMLWPGH"
     gene            1860..>2289
                     /locus_tag="VFV25_00410"
     CDS             1860..>2289
                     /locus_tag="VFV25_00410"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352735.1"
                     /note="One of the components of the high-affinity
                     ATP-driven potassium transport (or KDP) system, which
                     catalyzes the hydrolysis of ATP coupled with the exchange
                     of hydrogen and potassium ions; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit B"
                     /protein_id="HEY1391805.1"
                     /translation="MSTHHKSFSLFDPVLLRPAIWASFHKLDPRMQWRNPVMFVVYVG
                     SIITTLLWLQALGGHGEAPAGFILAIALWLWFTVLFANFAEALAEGRSKAQAASLRGM
                     KQRVIAKKLDEPRHGSSWHPMQAEELRRGAIVLVEAGDFIP"
BASE COUNT          360 a          762 c          744 g          423 t
ORIGIN      
        1 gcctgagcta tggcctggct cgccttgggg ggcccaagca atgagtaccc acgcctggct
       61 actcctgggg gtctattccc tcgtcctgct ggcgctatcc ctgcccatgg gccgctatct
      121 cgcctatgtc atggaggggc gcttcagctt cggccagcgc attgagcgcg tgctgtatcg
      181 catcaccggc gtgcaggccg acgaagaaat gggctggctg cgctacgcga tcggcctgct
      241 gctcttcaac ggcctgggcg tgctcgccgt ctatgcgctg cagcggctgc aggcctggct
      301 gccgctggac ccgcagcagt tctccaacgt cagcgcggat tcctcgttca acacggcggt
      361 gagctttgtc accaacacgg actggcaggg ttacacgccc gagcagacga tgagccatct
      421 cacgcagatg ctggggcttg cggtgcagaa cttcctgtct gccgccaccg gcatcgtggt
      481 ggtcatcgcg ctgatccgcg gcttcgcgcg caagagcgcc gagaccatcg gcaacctgtg
      541 ggtggacctc gcgcgcatca cgctgtgggt gctgctgccg atctgcacga tctacgcagt
      601 cttcctcgtg agccagggct cggtgcagag cttcaagggc ttcactgaag ccacgaccgt
      661 ggaagtcacg aagtaccagg agccggtgct cggcaaggac ggccagccgg tgaaggatgc
      721 caagggcaac ccggtgatga aggatgccca gaccgacaag cagcagctgc ccatgggccc
      781 ggtcgcctcg caggaagcga tcaagatgct gggcaccaac ggcggcggct tcttcaacgc
      841 caactcggcc cacccctacg agaacccgac ggcgctgacg aacttcgtgc agatgctgtc
      901 gatcttcgtg atccccggcg gcctgtgctt cgccttcggg atcatggtgg tcgatcggcg
      961 ccagggctgg accgtctatg tcgcgatgtt cctgctcttc gtggctggcg cgatcgccgc
     1021 cacccacttc gagcagcagg gcaatccgct gctggccaag ctcggcgtcg accagaccct
     1081 gagcgcgacc cagcccggcg gcaacatgga aggcaaggag acgcgcttcg gcatcgccga
     1141 ctccgcgctc ttcgccacca tcacgaccga tgcctcctgc ggcgccgtga acagcatgca
     1201 cgactccttc ctgccgctgg gcggcttcgt gccgctggtg aacatgcagt tgggcgaagt
     1261 ggtgttcggc ggcgtcggct cgggcctgta cggcatgctg gtgttcgcca tcgtcgccgt
     1321 cttcgttgcc gggttgatga tcggccgcac gcccgagtac ctgggcaaga agatcgagac
     1381 ctacgagatg aagatgacct cgatcgccgt gctcgtgacg ccgctgctcg tgctcgcact
     1441 ggcagccgtc gcggtgatga gcgagggcgg caaggccggc atcgccaacc ccggtgcaca
     1501 cggcttctcg gagatcctct acgccttcac ctcggtcgcc aacaacaacg gcagcgcctt
     1561 cgcgggcctg tcggccaaca cgcccttcta caacatcgtg ctggccgtgg gcatgtggtt
     1621 cggccgtttc ggcgtgatcg tgccggtgct ggccctggcc ggttcgctgg cggccaagaa
     1681 gcgcatcacg gcgggtgcag gcaccctgcc cacgcacggg cccctcttca tcgcgctgct
     1741 gatcggcacg gtgctgctgg tgggtctctt gaactatgtg ccggcgctgg ccctggggcc
     1801 ggtggtggag cacttcatgt tgtggcccgg ccattgagcg caccacagag agcgaaacca
     1861 tgagtacgca tcacaaatcc ttttcgctgt tcgatccggt cctgctgagg ccggccatct
     1921 gggcgtcctt ccacaagctg gacccgcgca tgcaatggcg caacccggtg atgttcgtcg
     1981 tttacgtcgg cagcatcatc acgacgctcc tgtggctgca ggcgctgggc ggccatggtg
     2041 aggcgccggc cggcttcatc ctggccatcg cgctgtggct gtggttcacg gtgctgttcg
     2101 ccaacttcgc ggaggcgctc gccgagggcc gcagcaaggc ccaggcggcc tcgctgcgcg
     2161 gcatgaagca acgcgtcatc gccaaaaagc tggacgaacc gcgccatggc tcctcctggc
     2221 atccgatgca ggccgaggaa ttgcgccgcg gcgccatcgt gctggtcgag gccggcgact
     2281 tcattccgc
//
LOCUS       DASUCY010000022         4767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_980363, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000022 DASUCY010000000
VERSION     DASUCY010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4767
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980363"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..153)
                     /locus_tag="VFV25_00415"
     CDS             complement(<1..153)
                     /locus_tag="VFV25_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY1391806.1"
                     /translation="MAEPLLVVEGLRCGHGEAVVLQALSFSIEAGRSLALLGRNGAGK
                     TTLIETL"
     gene            complement(146..901)
                     /locus_tag="VFV25_00420"
     CDS             complement(146..901)
                     /locus_tag="VFV25_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY1391807.1"
                     /translation="MSNVVLQTQGLTRRFGALPATDKVSLRIERGARHALIGPNGAGK
                     TTLINLLSGVLTPSEGSIALLGEDITALPAHRRVQRGLVRSFQINQLFDSLTPLQSLA
                     VAVSRRLGVSRRWWQPLGADRRLAARCEQLVRQFRLEDVAQQRTAELPYGKRRLLEIA
                     LALACEPRVLLLDEPVAGVPAGERREILDTLAALPGEVAVVLIEHDMDLVFSFAHRMT
                     VLVNGAVLIEGGPEQIAADPQVKAVYLGEGTLG"
     gene            complement(898..1875)
                     /locus_tag="VFV25_00425"
     CDS             complement(898..1875)
                     /locus_tag="VFV25_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY1391808.1"
                     /translation="MSGGLDVAAALAQSARWRAWEFAFWAAAALAPIALPQHAALINE
                     IASLALFALSLDLILGYAGIVSLGHAAFFGLGAYAAALFAKHVHPDPLLGLAVAVAVA
                     AALGALCSFTIVRGSDLTRLMVTLGVGLILYELANKFDALTGGADGLQGVVMGPLFGR
                     FEFDLLGHTAVAYSLAVLFLVFLLVRRLVHTPFGWSLQALRDNRLRAQAIGLSVRARL
                     VLVYTLAAAIAGAAGALLAQTTAFASLDLLDFHRSADVLLVLVIGGAGWLYGGLIGAV
                     VFKLMHDLLSAITPQYWTFWIGLFLVVLMLVGRERLLKPWTWRKRNGVA"
     gene            complement(1872..2744)
                     /locus_tag="VFV25_00430"
     CDS             complement(1872..2744)
                     /locus_tag="VFV25_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY1391809.1"
                     /translation="MLTLLFDGIAYGMLLFVLALGLAVTLGLMNFINLAHGAFAMAGG
                     YVMVLLMQRYGWPFLACLPAAFMVTALLGALLERTLYRPMYAKPHLDQVLFSIGLAFM
                     AIAAVDYFAGASQQIVQLPGWLRARYEFGSGAWLLGMGAYRLFIIAVCLVLALVLQAA
                     LARTRFGSRLRAAVDDPRVAAGLGIPVDRVFLLTFAAGSGLAGLGGALGAEVLGLDPG
                     FPLKFMVYFLIVVAVGGTSSITGPLLASLLLGIADVAGKYYLPKLGAFIVYTLMIVLL
                     LWRPHGLFSREGNR"
     gene            complement(2766..3941)
                     /locus_tag="VFV25_00435"
     CDS             complement(2766..3941)
                     /locus_tag="VFV25_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY1391810.1"
                     /translation="MNQKKRLVLNCLATCLAFAAAASACAQAASYKIGLILPMTGPSA
                     STGRQIEAAAKLYMAQNGASIAGRKVELIVKDDAGVADTTRRLAQELVVNDHVNVLAG
                     FGLTPTALATAPIATQSKTPMVVMAAATSSITEASPYIVRTSFTLPQVTLGIADWAPK
                     NGIRKVVTLVTDYGPGIDAEKTFKERFTLGGGQVLDSLRVPLRTPDFAPFLQKVRDAR
                     PDAVFVFVPSGFGASFMKQFDERGLRQAGIRLIATGDVLDDDQLNDIGDVALGIVNSH
                     HYSAAHDSPANKKFVAAFEAANKGMRPNFMAVGGYDGMHAIYHALAESKGAGGDAVLA
                     ALKGQVFESPRGPVLIDAQTRDIVQDVYIRKVERINGQLYNVEFETLKAVKDPGKAR"
     gene            4096..>4767
                     /locus_tag="VFV25_00440"
     CDS             4096..>4767
                     /locus_tag="VFV25_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY1391811.1"
                     /translation="MKSFGKKAQGLRLERMKASPRWVGEGFRNIHPVLPGLRDADAQR
                     PSLSEFLCGGARRVPRGPLPSVDPREAWSRKSGTGLRATWLGHSTVLIEIDGLRVLTD
                     PVWGPRASPSRIAGPKRFQPVPISLRKLPPIDLVIVSHDHYDHLDYPTIVELAKSEVP
                     FVTSLGVGAHLEAWGVPPQRITELDWWETHQLPKAELSITAAPSQHFSGRGLKDRNAT
                     LWSSMV"
BASE COUNT          825 a         1641 c         1636 g          665 t
ORIGIN      
        1 caaggtctcg atcagcgtgg tcttgccggc gccgttgcgg cccagcagcg cgagcgaccg
       61 gccggcctcg atgctgaagc tcaacgcttg cagcaccacg gcctcgccat ggccgcagcg
      121 caggccctca accacgagta gaggctcagc caagcgtgcc ctcccccagg tagacggcct
      181 tcacctgcgg atccgccgcg atctgctcgg gcccgccttc gatcagcacg gcgccattga
      241 ccagcaccgt catgcgatgc gcgaagctga agaccaggtc catatcgtgc tcgatcagca
      301 ccaccgcgac ctcgccgggc agcgccgcca gcgtatcgag gatctcgcgc cgctcgccgg
      361 ccggcacgcc cgccaccggc tcgtcaagca gcagcacgcg cggctcgcag gccagcgcca
      421 gcgcgatctc cagcaggcgg cgcttgccat agggcagctc ggcggtacgc tgctgcgcga
      481 catcctcgag gcggaactgg cgcaccagtt gctcgcagcg ggcggccagg cgccggtccg
      541 cgcccaacgg ctgccaccag cgcctggaga cgcccagacg ccgtgaaaca gccacggcca
      601 ggctctgcag cggcgtgagc gaatcgaaca gctggttgat ctggaagctg cgcaccaggc
      661 cacgctgcac ccgccggtgc gcaggcaatg ccgtgatgtc ttcgcccagt agcgcgatgc
      721 tgccttcgct gggagtgagc acaccgctga gcaggttgat tagcgtggtc ttgccggcgc
      781 cattggggcc gatcagcgca tggcgcgcgc cgcgctcgat gcgcagcgag actttgtcgg
      841 tcgcgggcag cgcgccgaag cggcgcgtga ggccctgggt ctgcagcacc acattgctca
      901 tgcgacgcca ttcctcttcc gccaggtcca aggcttcagc aagcgctcgc ggccgaccag
      961 catcagcacg acgaggaaga ggccgatcca gaaggtccag tattgcggtg tgatggccga
     1021 caacaggtcg tgcatcagct tgaagacgac ggcgccgatc aggccgccgt agagccagcc
     1081 cgccccgcca atcaccagca ccagcagcac atcggcgctg cggtggaagt ccagcaggtc
     1141 cagcgaggca aaggccgtgg tctgggccag cagggccccg gcggcgcccg cgatggcggc
     1201 ggccagggtg tagacgagca ccagccgcgc gcgcaccgac aggccgatgg cctgcgcgcg
     1261 cagccggttg tcgcgcagcg cctgcaagga ccagccgaag ggcgtgtgca ccaggcggcg
     1321 caccagcagg aagacgagga acaacacagc caggctgtag gccaccgccg tgtgccctag
     1381 caggtcgaac tcgaagcggc cgaagagcgg ccccatcacc acgccctgca gcccgtcggc
     1441 cccaccggtc agggcgtcga acttgttggc cagctcgtag aggatcaggc ccactcccag
     1501 cgtgaccatc agccgcgtca gatcggaacc gcgcacgatg gtgaagctgc acaacgcccc
     1561 gagtgccgcc gcaacggcga ccgctaccgc taggcccaac agcggatcgg gatgaacatg
     1621 cttggcgaac agggccgcgg cataggcgcc cagcccgaag aaggccgcat gcccgagcga
     1681 gacgatgccg gcatagccga ggatgagatc gagcgacagc gcgaacagcg ccaggcttgc
     1741 gatctcgttg atcaaggcgg catgctgcgg cagtgcaatg ggggccagag ccgccgcagc
     1801 ccagaacgcg aactcccagg cgcgccagcg cgcggactgt gcgagcgccg cggcgacgtc
     1861 gagcccgccg ctcatcggtt gccctcgcgc gagaacaggc catggggccg ccagagcagc
     1921 aagacgatca tcagcgtgta gacgatgaag gcgccaagct tgggcaggta gtatttgccg
     1981 gccacgtcgg caatgcccag cagcagcgag gcgagcagcg ggccggtgat ggagctggtg
     2041 ccgcccaccg ccacgacgat caggaaatac accatgaact tgagcgggaa gcccgggtcc
     2101 aggcccagca cctcggcgcc cagcgccccg cccaggccag ccaggcccga gccggcggcg
     2161 aaggtgagca ggaagacgcg atcgaccggg atgcccaggc ctgccgccac gcgcgggtcg
     2221 tccacggcgg cgcgcaggcg gctgccgaag cgcgtgcgtg cgagcgcggc ctgcagcacc
     2281 agcgcgagga ccagacagac cgcgatgatg aagagccggt aggcccccat gcccagcagc
     2341 caggcgccgg agccgaactc gtagcgcgcg cgcagccagc cgggcagctg cacgatctgc
     2401 tggctcgcac cggcgaaata gtccaccgcc gcgatggcca tgaaggcgag gccgatcgag
     2461 aacagcacct ggtccaggtg aggcttggcg tacatgggcc ggtacagcgt gcgttcgagc
     2521 agcgcgccca gcagcgcggt gaccatgaag gccgccggca ggcaagccag gaagggccag
     2581 ccgtagcgct gcatcagcag caccatcaca tagccccccg ccatcgcgaa ggcgccgtgc
     2641 gcgaggttga tgaaattcat caggcccagc gtcacggcca gcccgagggc gagcacgaac
     2701 agcagcatgc cgtaggcgat gccgtcgaag agcagcgtga gcatgcggcc ggcgcgaagc
     2761 gtggctcagc gggccttgcc cggatccttg accgccttca gcgtctcgaa ctccacgttg
     2821 tagagctggc cgttgatgcg ctcgaccttg cggatataga cgtcctgcac gatgtcgcgc
     2881 gtctgcgcat cgatcagcac cggcccgcgg gggctttcga agacctggcc cttgagcgcc
     2941 gccagcaccg catcgccgcc ggcccccttg ctctccgcca gcgcgtggta gatcgcgtgc
     3001 atgccgtcgt agccgcccac cgccatgaag ttgggacgca tgcccttgtt ggcggcctcg
     3061 aaggcggcga cgaacttctt gttggccggc gaatcgtggg ccgcggaata gtggtgcgaa
     3121 ttgacgatgc ccagggccac gtcgccgatg tcgttgagct ggtcgtcgtc cagcacgtcg
     3181 ccggtggcga tcagcctgat gccggcctgg cgcaggccgc gctcatcgaa ctgcttcatg
     3241 aagctcgcgc cgaagcccga aggtacgaag acgaagaccg catcgggcct ggcgtcgcgc
     3301 accttctgca ggaagggcgc gaagtccggg gtgcgcagcg gcacgcgcag gctgtccagc
     3361 acctggccgc cgcccagcgt gaagcgctcc ttgaaggtct tctcggcatc gatgcccggg
     3421 ccgtagtcgg tgaccagcgt gaccaccttc ctgatgccgt tcttgggtgc ccagtcggca
     3481 atgcccagcg tgacctgggg cagcgtgaag ctggtgcgca cgatataggg cgaggcctcg
     3541 gtgatgctgg aggtggccgc ggccatcacc accatcggcg tcttgctctg cgtggcgatg
     3601 ggcgctgtgg ccagtgccgt cggcgtgaga ccgaagcccg ccagcacgtt gacgtgatcg
     3661 ttgaccacca gttcctgcgc caggcggcgc gtcgtgtcgg ccacgccggc atcgtctttg
     3721 acgatgagct cgaccttgcg gccggcgatg ctggcgccgt tctgcgccat gtagagcttg
     3781 gccgctgcct cgatctggcg gccagtggag gccgaagggc cggtcattgg caggatcagg
     3841 ccgatcttgt agctcgccgc ctgggcgcag gcacttgccg cggcggcgaa ggccaggcag
     3901 gtggcaaggc agttcaggac gaggcgcttc ttctggttca tgcgtttctc ctgttgggcg
     3961 gcggcaaggc gccgcccgag catagcgccc cacggcccgg caagcgctcg ggactgatgc
     4021 gggtttggga gggctgtcga gcggccccgc ggcaccagtg gcagactggg gcatccagct
     4081 ttgcgagggg ttgcaatgaa gtcattcggc aagaaggcgc aggggctgcg gctcgaacgc
     4141 atgaaagcgt ccccgcgctg ggttggcgag ggcttccgca acatccatcc ggtgctgccc
     4201 ggccttcgcg atgcggacgc gcagcgcccc agcctgagcg aattcctctg cggcggagcc
     4261 aggcgcgtgc cgcgcggccc tctgccctcg gtagacccgc gcgaggcctg gtcgcgcaag
     4321 agcggcacgg ggctgcgcgc cacctggctc ggccattcga cggtgctgat cgagatcgac
     4381 ggtctgcgcg tgctcaccga cccggtctgg ggccccaggg cctcgccctc gcgcatcgcc
     4441 gggcccaagc gcttccagcc ggttccgatc tctttgcgca agctgccgcc catcgacctc
     4501 gtgatcgtct cgcacgacca ctacgaccat ctggactatc cgaccatcgt cgaactcgca
     4561 aagagcgagg ttcccttcgt cacctcgctg ggcgtaggcg cccacctcga ggcctggggc
     4621 gtgccaccgc agcgcatcac cgagctcgac tggtgggaaa cccatcagct gcccaaggcc
     4681 gagctgagca tcacggccgc gccgtcccag catttctcag gtcgcgggct gaaggatcgc
     4741 aatgcgacgc tgtggtcctc gatggtc
//
LOCUS       DASUCY010000023         2526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1012493, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000023 DASUCY010000000
VERSION     DASUCY010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012493"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1060
                     /locus_tag="VFV25_00445"
     CDS             <1..1060
                     /locus_tag="VFV25_00445"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEY1391812.1"
                     /translation="ELEIELVSGSPLALVDVAGRLIGKHGLWLDIRSKAERGDRLARG
                     IVEVPAIKSGDVALSEKVSVQEARAAVLASCLQQVLANASEIAAGLGGPEHVHQLRVG
                     LRRLRSAQRLFAGGSHDSHGDESGGQGAAAQPKAGASAAELFRSLGAARDRDVLVATL
                     VPALQAAHAPTLELPPAAQAVDPAALLRGREANLALLELLSLSLEAAEPPGPAADPAA
                     QALPPPLLRPLAAQQLKRWHRSIARDAARFTELDDAARHRLRKRLKRLRYSVEFVASL
                     FKRKRVAAYLGCLRPAQDALGFYNDLAIGEAAYRALLEQDAHAWFALGWIAARRAELL
                     GEAQLSLDALARAERFWK"
     gene            1306..1839
                     /locus_tag="VFV25_00450"
     CDS             1306..1839
                     /locus_tag="VFV25_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat U domain-containing protein"
                     /protein_id="HEY1391813.1"
                     /translation="MMKMLFKKTLVAAALLAAGGYAAAAASNPATAQFQVLMTVTKAC
                     TVTAANVSLGTADASAAVGTSGTNNISVTCSNKTGYNVALQSANNASNAGVGTLKGAA
                     SGNTDTLTYQLYSNSGMSTVWGNNGVTAAVTGNGVAGSGNGTAQSIGVWAKVTSAAPT
                     FVTPDSYSDTVTVSVYY"
     gene            1860..>2526
                     /locus_tag="VFV25_00455"
     CDS             1860..>2526
                     /locus_tag="VFV25_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HEY1391814.1"
                     /translation="MVLPLRRAAVMAGLWLACVAAGAGGLQISPVGLSLQPGQAADGL
                     WVTNAGDDVMHAQVRVYRWTQEGDADKLTPSQGLVASPPMLQLGVGEKQLVRAIRLGP
                     PPSGADAVEVAYRVVIDELPVSGEGKKGLNFVMRYSVPVFLVPAGATSALAGPHLSWA
                     LRRQGEHVQLEVSNSGSVHAQLADLSFVDAAGKRTALHSGLLGYVLPQARMRWLLKTP
                     PAIF"
BASE COUNT          401 a          865 c          860 g          400 t
ORIGIN      
        1 cgaactcgag atcgaactcg tcagcggcag cccgctggct ctcgtcgacg ttgccgggcg
       61 cctgatcggc aagcatggcc tgtggctcga catccgcagc aaggccgagc gcggcgaccg
      121 cctggctcgt ggcatcgttg aagtgccagc catcaagtcc ggggatgtgg cactgtcgga
      181 aaaggtgagc gtccaggaag cgcgcgctgc cgtgctggcc agctgcctgc agcaagtgct
      241 cgccaacgcc agcgagatcg ccgctgggct gggtgggcct gagcacgtgc atcagctgcg
      301 cgtcggcctg cgccggctgc gctcggcgca gcggctgttc gcaggcggca gccacgacag
      361 ccacggcgat gaaagcggcg ggcagggcgc ggccgcccag ccgaaggccg gtgcgagcgc
      421 ggccgaactg ttccgcagcc tgggcgcggc ccgcgaccgc gacgtgctcg tggcaacgct
      481 ggtgccggcc ctgcaggccg cacatgcgcc gaccctggag ctgccgccgg cggcgcaggc
      541 ggtcgatccc gcggccttgc tgcgggggcg cgaggccaat ctggccctgc tcgagctgct
      601 gtccctgagc ctggaggcgg ccgagccgcc ggggccggcc gcggatccgg cggcgcaggc
      661 attgccgccg cccctgctgc gtccgctcgc cgcgcaacag ctcaagcgct ggcaccggag
      721 catcgcgcgc gatgcagccc gcttcaccga gctcgacgac gcagcgcgcc accgcctgcg
      781 caagcgtctc aagcgcctgc gctactcggt cgaattcgtg gcctcgctgt tcaagcgcaa
      841 gcgggtggcc gcctatctcg gctgcctgcg cccggcccag gatgccctgg gcttctacaa
      901 cgacctcgcc atcggcgagg ccgcctatcg agccttgctg gagcaggacg cgcacgcctg
      961 gttcgcgctg ggctggatcg cggcgcgccg cgcggagttg ctcggcgagg cccagctgag
     1021 cctggacgca ctcgcccggg ccgagcgctt ctggaagtag gcgctcccgc agcctcgccg
     1081 ccgccgggga actgttcaga cagttgtcag gccttgcaag cctttgttca aagggcttga
     1141 atcgtgcaaa agtcgcacga ataatcatcg ggttttcgaa aagtcaagat gtcatttggg
     1201 gggatatctg taatcacatg tacagtaacc ctggtcgcag catcgatatc gatccgcaat
     1261 ggctgcgtcg gatacccgcc ccaaatcaaa gcaatcgtta ggagcatgat gaagatgctg
     1321 ttcaagaaga ccctggtcgc tgccgccctg ctggcagcgg gtggctatgc cgccgccgct
     1381 gcctccaacc ccgccacggc gcagttccag gtgctgatga ccgtcaccaa ggcttgcacg
     1441 gtaacggccg ccaacgtctc tttgggcacg gcggacgcct ccgcggcggt cggcaccagc
     1501 ggcaccaaca acatcagcgt gacctgctcg aacaagacgg gctacaacgt cgcgctgcaa
     1561 tccgccaaca acgcaagcaa cgctggcgtc ggcaccctga agggcgccgc cagcggcaac
     1621 accgacacgc tgacctatca gctctactcc aactcgggca tgagcaccgt ctggggcaat
     1681 aacggcgtta ccgccgccgt caccggcaac ggcgtggccg gcagcggcaa cggcacggcg
     1741 cagtccatcg gcgtgtgggc caaggtaacc agcgcggctc cgacctttgt cacgccggac
     1801 agctactccg acaccgtgac ggtcagcgtc tactactgac actgacgggt tgcccgaccg
     1861 tggtcctccc cttgcgccgc gcggcggtga tggccggtct ttggctggcc tgcgtagccg
     1921 ccggcgcggg ggggctgcag atttctccgg tgggcctgag cctgcagccg gggcaagctg
     1981 ccgacggcct gtgggtgacg aacgcgggcg atgacgtcat gcatgcccag gtccgcgtct
     2041 atcgctggac gcaggaaggc gatgccgaca agctcacacc ctcgcagggt ctggtggcca
     2101 gcccgccgat gctgcagctg ggggtcggcg aaaagcagct ggtccgtgct attcgcttgg
     2161 ggccgccccc cagtggggcc gatgccgtcg aagtcgccta ccgcgtcgtg atcgacgaat
     2221 tgccggtcag cggcgagggc aagaaggggc tcaacttcgt gatgcgctat tcggtgccgg
     2281 tctttctggt gccggccggt gccacatcgg ctctcgccgg tccgcacctg agctgggcgc
     2341 tgcgtcggca gggcgagcat gtgcagctgg aggtttccaa cagcggcagc gtgcacgccc
     2401 agctggccga tctcagcttt gtcgatgccg ccggcaagcg caccgcgctt cattccggcc
     2461 tgctgggtta tgtgctgccc caggcgcgca tgcgctggct gctcaagacg ccgccggcga
     2521 tattca
//
LOCUS       DASUCY010000024         3147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1398038, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000024 DASUCY010000000
VERSION     DASUCY010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1398038"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VFV25_00460"
     CDS             <1..433
                     /locus_tag="VFV25_00460"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY1391815.1"
                     /translation="TQSNPELGGPHANLGLIYGRAGKLPEAVAELEQATRLSPQQPQY
                     FNQLGIAYRRQGQFAKAREAYERAIALDPNYAAAYLNLGILSDLYLWDGQRALELYDR
                     YLALSPGGDAQVSKWVADLKNRKPQQQQQQRLSLLNRKEQE"
     gene            430..744
                     /locus_tag="VFV25_00465"
     CDS             430..744
                     /locus_tag="VFV25_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1391816.1"
                     /translation="MKPLPSLLSFALAFALALAGAAPALAQDQANIDATKIIGNRELP
                     KVLYIVPWKKPQPGDLSGRPVASVLDEALAPVDRDVFRRQVRYDAQVQPRAAAATSPK
                     RD"
     gene            789..1451
                     /locus_tag="VFV25_00470"
     CDS             789..1451
                     /locus_tag="VFV25_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEY1391817.1"
                     /translation="MNAFNTAVKFFQDCGLFIYPSLLIMALGVSIAIERFIFLARARS
                     ENRKVWAQIQPMLQSGKFKEVMGLTAQSDAAIGKIVNYGLVRMQSPGRREDIDASMEE
                     GMMLIVPRLEKRTHYIATFANVITLVGLLGTIIGLIKGFTAVAQVNPADKAEMLSASI
                     SIAMNNTAFALMVAIPFLLIHSFLQARTSEIVDGLEAAKVSFLNLVQRMRAEQGAAAG
                     AH"
     gene            1510..2001
                     /locus_tag="VFV25_00475"
     CDS             1510..2001
                     /locus_tag="VFV25_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY1391818.1"
                     /translation="MAVRRLHKKSAHLEITAFINLIVVLVPFLLSTAVFTRLAVIDLS
                     LPAQSANFDNLKGNDLKLEIVMRPDALEVGDRIGGLIQRIPSNDKGYDLRALSALMRQ
                     IKEKFPDKNEATILAELNTPYDNLVQVMDAVRTGKEARGAQIVSVELFPNISVGDAPV
                     SKH"
     gene            2011..2505
                     /locus_tag="VFV25_00480"
     CDS             2011..2505
                     /locus_tag="VFV25_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY1391819.1"
                     /translation="MKLTPLQRRAERRARNQTMVDMNLVSLIDVFTILIFFLLSNAGG
                     VELLPSPKAVKLPQSSSEKAPRDTVVVVVSGTDVVVEGRKVASVPDVMAAPGDVIAPL
                     KAELDLQANRQVIRKESQALSKTITIMGDKDIPYQLLRKVMVTCARANFSDVSFAVRQ
                     KVGS"
     gene            2502..>3147
                     /locus_tag="VFV25_00485"
     CDS             2502..>3147
                     /locus_tag="VFV25_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY1391820.1"
                     /translation="MSAATMVSFRTPVLAWSSTPEDEARFRRILARVLSAAVLLCLAM
                     LLLPPPKLDRSQPQELPPRLAKLVLERELAPTPSPVAKEPERSKEQQPQQSEAAKNPE
                     APKPQAARAEPVPKAARVPEARNPLPDRPPGEVDAARRKAAGVGLLAAADQLAELRGA
                     PVAVQLRQDIKQGPGVGTGVGSGVGAGTDPGVPTRALITSNATGGSGGINTAGYS"
BASE COUNT          577 a         1065 c         1016 g          489 t
ORIGIN      
        1 gacgcaatcc aaccccgagt tgggcggccc gcatgccaat ctaggcctga tctacggccg
       61 cgccggcaag ctgcccgagg ccgtggccga gttggaacag gcgaccaggc taagcccgca
      121 acaaccgcag tatttcaacc agctgggcat cgcctaccgg cgccaggggc agtttgccaa
      181 ggcgcgcgag gcctacgagc gcgcgattgc gctcgacccc aactacgcgg cggcctacct
      241 caacctgggc atcctgagcg atctctatct gtgggacggc cagcgcgccc tggagctcta
      301 tgaccgctat cttgcactgt cgccgggtgg tgacgcgcag gtgagcaaat gggtcgccga
      361 cctgaagaac cgcaagcctc agcagcagca acaacagcgc ttgagcctgt tgaaccgcaa
      421 ggagcaggaa tgaagccgct gccttccctc ttgtcattcg cgctggcatt cgcactggca
      481 ttggccggcg cggcgccggc cctggcgcag gaccaggcga acatcgacgc caccaagatc
      541 atcggcaacc gcgagctgcc caaggtcttg tacatcgtgc cctggaagaa gccgcagccc
      601 ggcgatctct ccggacgccc ggtggcgagc gtgctcgacg aggccctggc gcccgtcgac
      661 cgcgatgtct tccgccgcca agtgcggtac gacgcgcagg tccagccgcg cgcggcggca
      721 gcgacctcgc ccaagcgcga ttgagccctc tttcattcga gtccaaagac tatttcggag
      781 ccacagacat gaatgccttc aataccgccg tgaagttctt ccaggattgc ggcctgttca
      841 tctaccccag cctgctgatc atggccctgg gggtgtcgat cgccatcgag cgcttcatct
      901 tcctcgcgcg tgcacgcagc gagaaccgca aggtgtgggc gcagatccag cccatgctgc
      961 agagcggcaa gttcaaggag gtgatgggcc tcacggcgca gtcggatgcc gccatcggca
     1021 agatcgtcaa ctacggcctg gtccgcatgc agagcccggg ccggcgcgag gacatcgacg
     1081 cctcgatgga agagggcatg atgctaatcg tgccgcgcct ggagaagcgc acgcactaca
     1141 tcgccacctt cgccaacgtc atcacgctgg tgggcctgct gggcaccatc atcggcctga
     1201 tcaagggctt caccgcggtg gcccaggtga acccggcgga caaggccgag atgctgtcgg
     1261 ccagcatctc gatcgcgatg aacaacacgg ccttcgcgct gatggtggcc atccccttcc
     1321 tgctgatcca ctccttcctg caggcgagga ctagcgagat cgtcgacggc ctggaggcgg
     1381 ccaaggtcag cttcctgaac ctggtgcagc gcatgagggc cgagcagggc gcggccgccg
     1441 gggcgcattg atgcgcgaag cctcccctca tcctgatatg ccgctggcgc tgcgccggga
     1501 ggaatgacca tggccgttcg acgcctgcat aagaagtcgg ctcatctcga gatcaccgcc
     1561 ttcatcaacc taatcgtggt gctggtgccc ttcctgctgt ccaccgccgt gttcacgcgc
     1621 ctggcggtga tcgatctctc actaccggcc cagtccgcga acttcgacaa cctcaagggc
     1681 aacgatctga agctggagat cgtgatgcgg cccgacgcac tcgaagtcgg cgaccgcatc
     1741 ggcggcctga tccagcgcat tcccagcaat gacaagggct atgacctgcg ggcgctgtcg
     1801 gccctgatgc ggcagatcaa ggagaagttt ccggacaaga acgaagccac gatcctggcc
     1861 gagctcaaca cgccttacga caacctggtc caggtgatgg acgcggtgcg tacgggcaag
     1921 gaggcacgcg gtgcgcagat cgtgagcgtc gagctctttc ccaatatctc ggtcggcgat
     1981 gcgccggtca gcaagcatta gagcctcacc atgaagctga ccccgctgca aaggcgagcc
     2041 gagaggcgcg cccgcaacca gacgatggtg gacatgaatc tcgtgtccct catcgacgtg
     2101 ttcacgatcc tgatcttttt cctgctgtcc aacgcgggcg gcgtggagct gctgcccagc
     2161 cccaaggcgg taaagctgcc ccagtccagt tccgagaagg cgccgcgcga caccgtcgtg
     2221 gtcgtggtca gcggcaccga cgtcgtggtc gaagggcgca aggtcgcctc ggtgcctgat
     2281 gtgatggccg cgccgggcga cgtgatcgcg ccgctcaagg ccgagctgga tctgcaggcc
     2341 aaccgccagg tcatccgcaa ggagagccag gcgctgagca agaccatcac catcatgggt
     2401 gacaaggaca tcccctatca gctgctgcgc aaggtcatgg tcacctgcgc gcgggcgaac
     2461 ttcagcgacg tgtcctttgc cgtgcggcag aaggtgggat cgtgagcgca gccaccatgg
     2521 tttccttccg caccccggtg ctggcctggt ccagcacgcc ggaggacgag gcgcgctttc
     2581 gacgcatcct cgcgcgggtg ctgtcggcag cggtgctgct gtgccttgcg atgctgctgc
     2641 tgccgccacc caagctggac cgctcgcagc cgcaggagct gccgccgcgt ctagccaagc
     2701 tggtcctcga gcgcgagctc gcgcccacgc cgtcgccggt cgccaaggag ccggagcgca
     2761 gcaaggagca gcagccccag caaagcgagg ccgccaagaa tcccgaggct cccaagcccc
     2821 aggccgcgcg ggccgagcca gtgcccaagg cggctcgcgt gcccgaggcg cgcaacccgc
     2881 tgcccgatcg gccaccgggc gaagtggacg cagcaaggcg caaggccgcc ggcgtgggcc
     2941 tgctcgccgc ggcagatcag ctggccgagc tgcgcggcgc acctgtggcg gtacagctga
     3001 ggcaggacat caagcagggc cccggcgtag gcactggcgt cggatccggt gtgggggccg
     3061 gcaccgatcc cggtgtgccg acgcgcgcgc tgatcacctc caacgccaca ggcggcagcg
     3121 gcggcatcaa taccgcgggc tacagcc
//
LOCUS       DASUCY010000025         3122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1317752, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000025 DASUCY010000000
VERSION     DASUCY010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1317752"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VFV25_00490"
     CDS             <1..222
                     /locus_tag="VFV25_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY1391821.1"
                     /translation="GEVILMTLLGGLGTFAGPVVGAFTIIGLQDFLADRVGSWVTVII
                     GATFVVCVVAFRRGFVGELLAWQLRRKRA"
     gene            346..765
                     /locus_tag="VFV25_00495"
     CDS             346..765
                     /locus_tag="VFV25_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HEY1391822.1"
                     /translation="MKTEAALCTEQEVERLVHGFYERVRLDDELGPVFNVHISDWDHH
                     LAKMVDFWSSALRGTARYRGTPMPKHAALPGLSVALFKRWLGLFRETTAGLGNAAVQA
                     RADELALRIAQSLWYGYQMQRRPEHLPEDLPAEAARG"
     gene            758..2002
                     /locus_tag="VFV25_00500"
     CDS             758..2002
                     /locus_tag="VFV25_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006395245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEY1391823.1"
                     /translation="MAELLRIQEPSRPRAATLSWRALSSAFLEMAFRPLYLCGALWAA
                     LAIALWVFAPAWLSGELHGLAWHAHEMLWGFVATIAVGFLMTAGANWTGINPLRGRPL
                     GLVCALWALARLAFLVPGKSAFVTAAAAEFAFFAIAGIAMLAAVHKSRNRRNYGVPWL
                     LIALGAIDLLYLQAARGGDYPLLMQRFDAGLLCMAVLALLVARRVIPFFAMRAVPGLV
                     IPMHTRSGHWQLGFSALAVLLTLFNAQPWTAPALGAAGAIALLQLLAWRPWRVRGKPL
                     LWILYLGYALLGLGLLAAAAHDAGLALRAAWPVHIIAVGGFSVLIIGMITRTALGHLG
                     RALQLDRSMLASYWLMLVACALRLAALLSTALAASLLQAAALCWIAALSLYLWRFAPL
                     MIRPRPDAKSSLAAMPVKVAVR"
     gene            1999..2343
                     /gene="fdxA"
                     /locus_tag="VFV25_00505"
     CDS             1999..2343
                     /gene="fdxA"
                     /locus_tag="VFV25_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HEY1391824.1"
                     /translation="MTHVVTEACIRCKYTDCVAVCPVDAFREGKNMLVIAPEDCIDCR
                     LCIPECPVAAIFPEEELPQDQRDFIAINASLAKRWRVISRVKAAPPDADAWAKVAVKK
                     HLLDERGNHEEA"
     gene            2389..>3122
                     /locus_tag="VFV25_00510"
     CDS             2389..>3122
                     /locus_tag="VFV25_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY1391825.1"
                     /translation="MDSALLSLLGAFVLSIIGLFAFIWSLRQGLLIENPKAASVIFAR
                     GEIGRVDDPALAAPVQDAMQSAASAHGAVPQSADAGELEDRIAADRSTAFPVFMFIAF
                     ACFWLLIGSAAGLVASVKLHQPDWLVSEAWMSFGRLRTVHLNAVIYGWSSNACLAVIL
                     WLLPRLLRTPLWGSLWTMLGGTFINAGIASGIGAIAAGWTDGMEYLEIPWQIGIFIFI
                     GFALIIVPVLYTLVNRKVEHLYVSVW"
BASE COUNT          432 a         1055 c         1035 g          600 t
ORIGIN      
        1 ggcgaagtga tcctgatgac cctgctgggc ggcctgggca ctttcgccgg cccggtggtg
       61 ggtgccttca ccatcatcgg cctgcaggac ttcctggccg accgcgtggg ctcctgggtc
      121 acggtgatca tcggcgcgac cttcgtggtc tgcgtcgtcg ccttccgccg cggcttcgtc
      181 ggcgaactgc tggcctggca gctgcgccgc aagcgcgcct gagcgccccc aaacctgccg
      241 cttcgcttca ggcccccgag ggggtcaacc ggtcattgat tccgaatgga ttgacaggcc
      301 agccgtttgc attaagatat gcggtaaata tatctttaat gcgatatgaa gacagaagca
      361 gcgctgtgca ccgagcagga agtcgagcgg ctggtccacg gtttttacga gagggtgcgc
      421 ctcgacgatg agctgggccc cgtcttcaac gtccatatct ccgactggga tcatcatctg
      481 gccaagatgg tggacttctg gtcttcggcg ttgcgcggca cggctcgcta ccgcggcacg
      541 cccatgccca agcacgcggc cctgccaggc ctgagcgtgg cgctgttcaa gcgttggctg
      601 ggcctgttcc gcgagaccac ggccggcctg ggcaatgctg ccgtgcaggc gcgcgccgac
      661 gagctcgcgc tgcgcatcgc gcaaagcctc tggtacggct accagatgca gcgcaggccc
      721 gagcatctgc cggaagattt gcccgcagag gccgctcgtg gctgagttgc tgcgcatcca
      781 ggagccgagc cggcctcgag cagcgacgct gagctggcgt gccctttcga gtgctttctt
      841 ggaaatggcc ttccggcccc tctatctgtg cggcgcgttg tgggctgcgc tggccatcgc
      901 gctgtgggtc tttgctccgg cctggctgag cggcgagctg cacggcctgg cctggcatgc
      961 gcatgagatg ctgtggggct tcgtcgcgac gatcgcggtc ggcttcctga tgaccgcagg
     1021 cgccaactgg accggcatca acccgctgcg cggccgtccg ctgggcctgg tctgcgcgct
     1081 gtgggcactg gcgcgcctgg cttttctcgt gcctggcaaa agcgccttcg tcaccgcggc
     1141 ggcagccgaa ttcgccttct tcgccatcgc cggcatcgcg atgctggcgg ccgtgcacaa
     1201 gagccgcaac cggcgcaact atggcgtgcc ctggctgctg atcgcgctgg gcgcgatcga
     1261 cctgctttac ctgcaggcgg cgcgcggcgg cgactaccca ttgctgatgc agcgcttcga
     1321 tgccggcctg ctgtgcatgg ccgtgctggc cttgctggtg gcgcgccgcg tgattccctt
     1381 cttcgcgatg cgcgccgtgc ccggcctcgt gattcccatg cacacgcgca gcggccattg
     1441 gcagctgggc ttcagcgcgc tggcggtgct gctgacgctc ttcaacgccc agccctggac
     1501 tgcgccggcg ctcggcgcgg ctggcgccat cgcgctgctg cagctgctgg catggcgacc
     1561 gtggcgcgtg cgcggcaagc cgctgctgtg gatcctctac ctcggctatg cccttctggg
     1621 cctcggcctg ctcgccgcgg ctgcgcatga cgcaggcctg gcgctgcgcg ccgcctggcc
     1681 ggtgcacatc atcgcggtgg gcggcttctc ggtgctcatc atcggcatga tcacgcgcac
     1741 cgcgctgggc catctcgggc gagccctgca gctggaccgc agcatgctgg ccagctattg
     1801 gctgatgctg gtcgcctgcg cgctgcgtct ggccgccctg ctgtccacag cgcttgccgc
     1861 ctcgttgctg caggcggcgg ccttgtgttg gatcgccgct ctgagcctct atctgtggcg
     1921 tttcgcgccg ctgatgatcc gcccgcgtcc agacgcaaaa agcagcctcg cagcgatgcc
     1981 ggtcaaggtg gccgtgcgat gacccatgtc gtgaccgagg cctgcatccg gtgcaagtac
     2041 accgattgcg tggccgtctg cccggtcgac gcctttcgcg aaggcaagaa catgttggtc
     2101 atcgcccccg aagactgcat agactgccgg ctgtgcatcc ccgagtgtcc agtggcggcg
     2161 atctttcccg aggaagagct gccgcaggac cagcgcgact tcatcgcgat caacgcgagc
     2221 ctggccaagc gctggcgcgt gatctcgcgc gtgaaggcag cgcccccgga cgccgacgcc
     2281 tgggccaaag ttgcagtcaa gaagcatctg ctcgacgagc gaggcaatca tgaagaagcc
     2341 tgaatcattc ccgcgaggag ccgcaacgac gaaccaggag acgaggtgat ggacagcgca
     2401 ctcttgagtc tgcttggcgc tttcgtgctg tccatcatcg gcctgttcgc cttcatctgg
     2461 tcactgcgcc agggcctgct gatcgagaac ccgaaggcgg cctcggtgat cttcgcgcgc
     2521 ggcgagatcg gccgggtcga cgaccccgcg ctggccgcgc cggtgcagga cgcgatgcaa
     2581 agcgccgcca gtgcccatgg cgccgtgccc cagagcgcgg atgccggcga gctcgaagac
     2641 cgcatcgctg ccgaccgctc gaccgccttt ccggtcttca tgttcatcgc cttcgcctgc
     2701 ttctggctgc tgatcggctc ggccgccggc ctggtggcct cggtcaagct gcaccagccg
     2761 gactggctgg tgagcgaggc ctggatgagc ttcggccggc tgcgcaccgt gcatctgaac
     2821 gcggtgatct acggctggtc gtccaacgcc tgcctggccg tcatcctgtg gctgctgccg
     2881 cggctgctac gcacgccgct gtggggctcg ctgtggacca tgctgggcgg caccttcatc
     2941 aatgccggca tcgcctcggg cataggtgcc atcgccgcgg gctggaccga cggcatggaa
     3001 tacctcgaga tcccctggca gatcggtatc ttcatcttca tcggcttcgc cctcatcatc
     3061 gtgccggtgc tctacacgct ggtgaaccgc aaggtcgagc atctctacgt cagcgtgtgg
     3121 ta
//
LOCUS       DASUCY010000026         2582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_659137, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000026 DASUCY010000000
VERSION     DASUCY010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659137"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VFV25_00515"
     CDS             <1..301
                     /locus_tag="VFV25_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEY1391826.1"
                     /translation="AVLQRYKELRDIIAILGMDELSPEDKLAVARARKIQRFLSQPFH
                     VAEVFTGTPGKYVPLKETIRGFKMIVNGECDSLPEQAFYMVGTIDEAFEKAKTLQ"
     gene            381..797
                     /locus_tag="VFV25_00520"
     CDS             381..797
                     /locus_tag="VFV25_00520"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEY1391827.1"
                     /translation="MATIHVDVVSAEESIFSGEAKFVALPGETGELGILPKHTPLITR
                     IRPGAVRIERAEDGGEEFVFVAGGILEVQPNSVTVLADTAIRGKDLDEAKATEAKRLA
                     EEAMRNAKSDFDLAKAQSEFATMAAQIAAMRRFRKK"
     gene            complement(887..1396)
                     /locus_tag="VFV25_00525"
     CDS             complement(887..1396)
                     /locus_tag="VFV25_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEY1391828.1"
                     /translation="MHRLLRLLKHRWHDERDAHRALGPGALERLTEKVKASEARHSGE
                     IRICVEGGLPLSYLRRHATARERAITLFGKLRVWDTEHNNGVLIYLLLAERCIEIVAD
                     RGLSRHVSQAQWDEVVQRASAALQTSRSIEAGLATALDAVTELLVAHFPLKPGEARSN
                     ELPDAPVIT"
     gene            complement(1407..2306)
                     /locus_tag="VFV25_00530"
     CDS             complement(1407..2306)
                     /locus_tag="VFV25_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEY1391829.1"
                     /translation="MLLRRLWLIAVLLMGAAAAAQDLQPIPPLSARVIDQTATLSAAQ
                     QQALEQKLAAFEQASGPQIVVLLVTTTQPEDITDYTQRLGDAWKLGRRDVGDGLLIVV
                     AKNDRKVRIAPAKALEGAVPDLAARQIIDRAIVPAFRAGDYAGGLNAAVDQLMARIRG
                     EQLPAPAQRVSRHDGGWAWDQLLMFFFIGVPVAGSVLTAMMGRKLGSFVTALGAGGLA
                     WVLTASVLLAGAAGLLGLILVGLLGIGSARSGRGGPIIWGGGGFGGGGGGGGGGFGGG
                     GGGGGFSSGGGGDFGGGGSSGSW"
     gene            complement(2336..>2582)
                     /locus_tag="VFV25_00535"
     CDS             complement(2336..>2582)
                     /locus_tag="VFV25_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEY1391830.1"
                     /translation="LKTNQGFQDLRVQLEGTENRITVARNRYIKAVQDYNVLARSFPS
                     NLTAMMFSYQPKPNFTVQNEAQISTPPTVSFDKPASK"
BASE COUNT          424 a          897 c          850 g          411 t
ORIGIN      
        1 ggccgtgctg cagcgctaca aggaactgcg cgacatcatc gcgatcctgg gcatggacga
       61 gctgagcccg gaagacaagc tggccgtggc ccgcgcccgc aagatccagc gcttcctgtc
      121 gcagcccttc cacgtggccg aggtgttcac cggcacgccg ggcaagtacg tgccgctgaa
      181 ggaaaccatc cgcggcttca agatgatcgt caacggcgag tgcgacagcc tgccggagca
      241 ggccttctac atggtcggca ccatcgacga ggccttcgag aaggctaaga ctctccagtg
      301 acttgtgacc tgtgatcggt gacccggacg caattcctgg tcaccagtca cgagtcactg
      361 gtccctcccg aagggaaaac atggcaacca tacatgtcga cgtcgtttcc gccgaggagt
      421 cgatcttctc tggcgaggcg aagttcgtcg cgctgccggg cgagaccggc gagctgggca
      481 tcctgcccaa gcacacgccg ctgatcactc gcatccgccc gggcgcggtg cgcattgagc
      541 gggccgagga cggcggggaa gagttcgtgt tcgtggccgg cggcatcctc gaggtgcagc
      601 cgaactcggt cacggtgctg gccgataccg cgatccgcgg caaggatctc gacgaagcca
      661 aggccaccga ggccaagcgc ctggccgaag aggcgatgcg caacgccaag agcgacttcg
      721 acctggccaa ggcccagagc gagttcgcga cgatggcggc ccagatcgcc gcgatgcgcc
      781 gcttccgcaa gaagtaggct agtagccgcg tcgcggtcac cccgcgctac gaccacaagg
      841 cccgcccggt caccgccgag gcgggcttcg tatttctgcg ctggccctag gtgatcaccg
      901 gcgcgtccgg caattcgttg ctccttgcct cgccgggctt gagcgggaag tgggcgacga
      961 gcagctcggt gactgcgtcc aaggctgtgg cgaggccggc ctctatgctg cggctggtct
     1021 gcagcgcggc cgaagcgcgc tgcacgactt cgtcccattg cgcctggctc acatggcggc
     1081 taaggccgcg gtcggcgacg atttcgatgc agcgctcggc cagcagcagg tagatcagca
     1141 cgccgttgtt gtgctcggtg tcccagacgc gcaacttgcc gaacagcgtg atcgcgcgtt
     1201 cgcgcgccgt tgcgtggcgg cgcagatagc tcagcggcag cccgccctcg acgcagatgc
     1261 ggatttcgcc gctgtggcgg gcttcgctgg ctttgacctt ctcggtcagg cgctcgagcg
     1321 cacccggccc cagcgcgcgg tgcgcatcgc gttcgtcatg ccagcgatgc ttcagcaggc
     1381 gcagcaagcg gtgcatgctt ttccccttac cagctgccgg aggagccgcc gccgccgaag
     1441 tcgccaccgc ccccggacga gaagccaccc ccaccgcctc cgccgccgaa gccgcctccg
     1501 cctccacccc cacctccgcc gaacccaccg cctccccaga tgatcggtcc cccccggcca
     1561 ctgcgcgccg agccgatgcc cagcaagccg accaggatca gccccaggag cccggcggcg
     1621 ccggccagca acacgctcgc cgtcaacacc caggcgaggc cgccggcgcc cagcgcagtc
     1681 acgaaggagc ccagcttgcg gcccatcatc gcagtcagca cgctgccggc gacgggcaca
     1741 ccgatgaaga agaacatcag caactggtcc caggcccagc cgccatcatg ccgcgacact
     1801 cgttgcgcag gggcgggcag ctgctcgccg cggatgcggg ccatcagctg gtccaccgcg
     1861 gcgttgagcc cgccggcata gtcgccggcg cgaaaggccg gcacgatggc gcggtcgatg
     1921 atctggcgcg ccgccaggtc gggcaccgca ccttccagcg ccttggcggg cgcgatgcgc
     1981 accttgcggt cgttcttggc caccacgatg agcaagccat cgccgacatc gcgccgcccc
     2041 agcttccagg catcgcccag gcgctgggtg tagtcggtga tgtcctcggg ctgagtggtt
     2101 gtcaccagca gcacgacgat ctgcgggccc gaggcttgct cgaaggctgc gagcttttgt
     2161 tccagtgcct gctgctgcgc ggcagaaagc gtcgccgtct ggtcgatcac gcgcgcgctg
     2221 agcggcggga tgggctgcag gtcctgggca gccgccgctg cgcccatcag cagcacagct
     2281 atcagccaca agcggcgcag cagcatgggt ccagctcgtt gggttgcgca gtcgtctact
     2341 tcgaggccgg cttgtcgaag ctcaccgtcg gtggtgtgct gatctgggcc tcgttctgca
     2401 ccgtgaagtt gggcttgggc tgatagctga acatcatcgc cgtcaaattg ctcgggaaac
     2461 tgcgagccag cacgttgtag tcctgcacgg ccttgatgta gcgattgcgc gccaccgtga
     2521 tgcggttctc ggtgccctcg agctgcacgc gcaggtcctg gaagccctgg ttggtcttca
     2581 gg
//
LOCUS       DASUCY010000027         6227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_96848, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000027 DASUCY010000000
VERSION     DASUCY010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 6227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6227
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96848"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /locus_tag="VFV25_00540"
     CDS             complement(<1..1202)
                     /locus_tag="VFV25_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HEY1391831.1"
                     /translation="MSAYFRSAAQSRESVLARYSDLVLVAGIVAIVAMMVLPLPLWLI
                     DLLVAANIASGLLLLLLAIYIGSPLEFSVFPSVLLMTTLFRLSLSIATTRMILLRGDA
                     GHIITTFGKVVAGGNLVVGLVVFLIITVVQFIVIAKGAERVAEVAARFSLDAMPGKQL
                     SIDSDLRSGLIDKDEAKRRRRSLELESKLHGSLDGAMKFVKGDAIAGIVIIVINLLGG
                     LAIGVMQQGLDIATAMSKFSILTVGEGMVAQIPALLGAMSAGLIVTRTTDEEHDKHLG
                     DAIRKQITAKPRVLLVAGGICLLMSLVPGFPVGAFLALALGSAGSGMLLMPACRERIR
                     GLRHPAVRHLVQRLEPLPQVVATALPQPQPSVALLLEVPAGLLADGGAAVLTHALEGV
                     LEEIQLGLG"
     gene            complement(1205..1987)
                     /locus_tag="VFV25_00545"
     CDS             complement(1205..1987)
                     /locus_tag="VFV25_00545"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY1391832.1"
                     /translation="MQLRSDHRKDRGRAMLPCAVLLAAQMGLAGAQTCGGPLPDPGAT
                     LPASSEKFRLVARQCDVLAEPLQLQRAAQLDLYDRGASVTIRMSRDAPPPAREASPPP
                     QQPLPPAGQRVLSLAPALTAAAQEYGLDPLLLHAIAHVESRHNTRAVSRAGARGVMQV
                     MPATGRRFGIDDPERSLLEPHVNVHVGAVYLHTLLQRYGNDLQRVLAAYNAGEGAVDK
                     HGGVPPYPETQSYVREVLAVYRRLTAEFRVSPGGEITKREMT"
     gene            2189..3385
                     /gene="sctU"
                     /locus_tag="VFV25_00550"
     CDS             2189..3385
                     /gene="sctU"
                     /locus_tag="VFV25_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctU"
                     /protein_id="HEY1391833.1"
                     /translation="MADKNDGGDKTEKPTPKRLRDARKKGDVSKSREVTSTLVLLAWL
                     GVGALALGLATARIAALFEALFATLAQGWASGDYVTVLRALGAQAMELGALLVAMLLV
                     PAAAVGLLADFLQAGPVLSFEKIKPKLDHLNPVQGIKRMFSLDNLVEVLKAVLKTALL
                     FAIGWLVLRSALPDIVGLARSPQQPPRAIGALIWSLTLKAVAWTLVTFALVSLLDAAY
                     QRYSFIKKMRMSLRDIKQEMKESEGDPIVKQQRRQAHQEWSQRNAVQAARSANVVVVN
                     PTHVAIAIDYERETTPVPTIAAKGEDDLAHAMREAAEAAGVPIVRNVPLARDLLARGE
                     VGEIVPGDLFEIIAEVILWAREVRAEVDAQRQAAGGGPVVVQPRPTRRIVPPGEDLTH
                     YPQHRP"
     gene            3388..3678
                     /locus_tag="VFV25_00555"
     CDS             3388..3678
                     /locus_tag="VFV25_00555"
                     /inference="COORDINATES: protein motif:HMM:NF016250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HEY1391834.1"
                     /translation="MDSLLMSFASMLLALGAVIALAWVVLRFMRARMSPQGSRRGGAD
                     DALRFVRALPVGAKERVVIVEHRGQRWMLGVTAGAISTIAHWGAQADHEDRS"
     gene            3874..4488
                     /locus_tag="VFV25_00560"
     CDS             3874..4488
                     /locus_tag="VFV25_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1391835.1"
                     /translation="MSSALNSVFGGGGILGAALNIASLAFPELAICNSLANLLTQAIG
                     QAVNQAVQQLTQLCGMPKFIAQEISDVVKQALSGLQQQSDPDCDQQVQNQCGGGFSDF
                     ATELCKDIVNYAAQHMGGEKAKGKGAGSWFEALAQALGQALDKQADKIKNLSSQVTDD
                     NAKDKPSTMTQLQSESQRMSFMMSAADQVLKTLGEALSNMARKQ"
     gene            4564..4908
                     /locus_tag="VFV25_00565"
     CDS             4564..4908
                     /locus_tag="VFV25_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1391836.1"
                     /translation="MSLPIDIVGSLWFMPTAIWEWEPEAGPAPGSQGAAGCADGGATG
                     GEAVADQHYGMRLVFKGYANTASFHWFAADSLLSRPWTRDDADDAHRRRATERTPRVD
                     EVPRADDDAKSS"
     gene            5058..5279
                     /locus_tag="VFV25_00570"
     CDS             5058..5279
                     /locus_tag="VFV25_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1391837.1"
                     /translation="MIRVSDYVNVAAWTRSLSAPARTEAKDPRSWFEGDAADVDVRID
                     GTPTARGLNIEQHAGRVLACLCGERDAAG"
     gene            5295..5957
                     /locus_tag="VFV25_00575"
     CDS             5295..5957
                     /locus_tag="VFV25_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1391838.1"
                     /translation="MAALLSEPNWVGAAQALVDGCADLPNDDDRVALLIAVCQGLGDE
                     LYPAFIRVLWTIGRHGEHAAKAAVACALVHALRTGRLPSGRRSAWGASAPMNALGSYG
                     RTRCLGPLEYLCAWHAQTEPASALTSAQFHVAACALMELIACSREARLLYCEKLLGDV
                     DDPIGGALARYTRDALRALAIAWSEGASAWDASARFICTLRRPSPDVLATLAEQSAPA
                     LR"
     gene            complement(6004..6159)
                     /locus_tag="VFV25_00580"
                     /pseudo
     CDS             complement(6004..6159)
                     /locus_tag="VFV25_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489725.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         1056 a         2066 c         2189 g          916 t
ORIGIN      
        1 ccgaggccga gctggatctc ttcgagcaca ccctccagcg cgtgcgtcag caccgcggcg
       61 ccaccatcgg ccagcagccc ggccggcact tcgagcaaca gcgcgaccga gggctgcggc
      121 tgcggcagcg cggtggcgac cacctgcggc agcggctcca gcctctgcac gagatggcgc
      181 accgccgggt ggcgcaggcc gcggatgcgc tcgcggcagg cgggcatcag cagcatcccc
      241 gagccggcgg agcccagcgc gagcgccagg aaggcgccga ccggaaagcc gggcaccagc
      301 gacatcagca ggcagatgcc gccggccacc agcagcacgc gtggcttggc cgtgatctgc
      361 ttgcggatcg cgtcgcccag gtgcttgtcg tgctcctcgt cggtggtgcg ggtgacgatg
      421 agaccggccg acatcgcgcc cagcagcgcc gggatctgcg cgaccatgcc ttcgccgacg
      481 gtgaggatcg agaacttcga catcgcggtc gcgatgtcca gcccttgctg catcacgccg
      541 atggccaggc cgccgagcag gttgatgaca atgatcacga tgcccgcgat cgcatcgcct
      601 ttgacgaact tcatcgcgcc atcgaggctg ccgtgcagct tgctctccag ctcgagggag
      661 cggcgccgcc gcttggcctc gtccttgtcg atcaggcccg agcgcaggtc ggagtcgatc
      721 gacagctgct tgccgggcat cgcatcgagc gagaaccttg ccgccacctc ggccacgcgc
      781 tcggcgccct tggcgatgac gatgaactgc accaccgtga tgatgaggaa caccaccagg
      841 ccgacgacca gattgccgcc cgccaccacc ttgccgaagg tagtgatgat gtggccggcg
      901 tcgccacgca gcaggatcat gcgcgtggtc gcgatcgaca gcgacaggcg aaacagtgtg
      961 gtcatcagca gcacgctggg aaacaccgaa aactccagcg gcgatccgat gtagatcgcc
     1021 agcagcaaca gcagcaggcc cgaggcgatg ttggccgcca ccagcaggtc gatcagccac
     1081 agcggcagcg gcagcaccat cattgcaacg atggcgacga tgccggccac cagcaccagg
     1141 tcgctgtagc gcgcgagcac gctctcgcgc gactgcgcag cgctgcggaa gtaggccgac
     1201 atggtcatgt catctcccgc ttcgtgatct cgcccccggg cgagaccctg aactcggccg
     1261 tgaggcgccg gtagacggcc agcacttcgc gcacatagga ctgggtttcc ggatagggcg
     1321 gcacgccgcc atgcttgtcc acggcgccct cgcctgcgtt gtaggcggcc agcacgcgct
     1381 gtagatcgtt gccgtagcgc tggagcagcg tgtgcagata gaccgcgccg acgtgcacat
     1441 tgacatgcgg ctccagcagc gagcgctcgg ggtcgtcgat accgaagcgg cggccggtgg
     1501 ccggcattac ctgcatcacg ccgcgtgcgc cggcgcgtga cacggcgcgg gtgttgtggc
     1561 gcgactcgac gtgggcgatc gcatgcagca gcagcgggtc gagcccgtat tcctgggccg
     1621 cggctgtcag cgcaggcgcg agcgacagca cgcgctggcc agcgggcggg agcggctgct
     1681 gagggggagg gctcgcctct cgggcaggag gcggcgcgtc gcgcgacatg cggatggtga
     1741 ccgaagcgcc gcggtcatac aggtcgagct gcgccgcgcg ctgcagttgc agcggctcgg
     1801 cgagtacgtc gcactgccgc gcgacgaggc ggaacttctc gctgctcgcg ggcagcgtcg
     1861 ccccgggatc gggcagcggt ccgccacaag tctgcgcgcc cgcgagcccc atctgcgcag
     1921 ccagcagaac tgcgcacggc agcatcgcgc ggcctcggtc tttgcggtga tcggatcgca
     1981 gttgcatggc agacccgcct ccttccgcgg gggaaatcgc atgtgccggc tgtgttgcac
     2041 ggcctgcgcg acaggttcat gcgaattacg cgacaagcct gcgtgccatg cgcaggtgac
     2101 taggacaagc cccaactggg agtgaaccta gccagcattc accgcaactg acgcgtggag
     2161 ccacatgggg tggcgaagga ccgcgaccat ggccgacaag aacgacggcg gcgacaagac
     2221 cgagaagccg acacccaagc gcctgcggga cgcacgcaag aagggcgacg tttccaagag
     2281 ccgcgaggtg acgagcacgc tggtgctgct cgcctggctc ggcgtcggcg cgctcgcact
     2341 gggcctcgct acggcgcgca tcgctgcgct gttcgaggcc ttgttcgcca cgctggccca
     2401 gggctgggcc agcggcgact atgtgacggt gctgcgcgcg ctcggggccc aggcgatgga
     2461 gctcggcgcg ctgctggtgg cgatgctgct cgtgccggcc gccgcggtcg gactgctggc
     2521 cgatttcctg caggccgggc cggtgctcag cttcgagaag atcaaaccca agctcgacca
     2581 cctgaacccg gtgcagggca tcaagcgcat gttctcgctg gacaacctgg tcgaggtgct
     2641 gaaggcggtg ctgaagacgg ccctgctgtt cgccatcggc tggctggtgc tgagatcggc
     2701 gctgcccgat atcgtgggcc tcgcgcgctc gccgcaacag ccgccgcgtg ccatcggcgc
     2761 gctgatctgg agtctcacgc tgaaggccgt ggcctggacg ctggtcactt tcgcgctggt
     2821 gtcgctgctc gatgccgcgt accagcgcta ctcgttcatc aagaagatgc gcatgagcct
     2881 gcgcgacatc aagcaggaga tgaaggagag cgagggcgac cccatcgtca agcagcagcg
     2941 tcggcaggcc catcaggaat ggtcgcagcg caatgccgtg caggcggcac gcagcgccaa
     3001 cgtggtggtg gtgaacccca cccatgtggc gattgcgatc gactacgagc gcgagaccac
     3061 cccggtgccg accatcgccg ccaagggcga ggacgatctc gcgcacgcga tgcgcgaggc
     3121 ggcagaggcg gccggcgtgc ccatcgtgcg caacgtgccg ctggcgcgcg acctgctggc
     3181 gcgcggcgag gtcggtgaga tcgtgccggg cgacctgttc gagatcatcg ccgaggtgat
     3241 cctctgggcg cgcgaggtgc gcgccgaggt ggatgctcag cggcaagccg ccgggggcgg
     3301 acccgtcgtt gtccagccgc gtccgacgcg ccgcattgtg ccgcccggcg aagacctgac
     3361 tcattaccct caacacaggc cctgaacatg gattcactgc tgatgagctt cgccagcatg
     3421 ctgctggcct tgggcgccgt catcgcgctg gcatgggtcg tgctgcgctt catgcgcgcg
     3481 cgcatgtcgc cgcagggttc gcggcgcggc ggggcggatg atgcgctgcg cttcgtgcgc
     3541 gcgctgccgg tgggtgcgaa ggagcgagtg gtgatcgtcg agcatcgcgg gcagcgctgg
     3601 atgctgggcg tcaccgccgg cgccatcagc accatcgcgc actggggcgc gcaggcagat
     3661 catgaggacc gctcctagct gcgggctgcc ctagctgccg tacgcaccga ctgtcgcgcg
     3721 gggtttgcgt cgcctttaat tggttcgtcg gcggcgcgat cgcatcaata ccgcaggcat
     3781 cgagagatcc gcggcacagc gccgcgggac acggcaaccc ggggcgcgat agagcatcat
     3841 cgatgcgccc ctgacgacag ttaggagcac atcatgtctt cagcattgaa cagcgtcttc
     3901 ggcggcggcg gcatcctcgg agcggcactc aacatcgcaa gcctcgcgtt tcccgagctg
     3961 gccatctgca actccctcgc caacctcctg acccaggcca tcggccaggc agtgaaccag
     4021 gccgtgcagc agctgaccca gctgtgcggc atgccgaagt tcatcgcgca ggagatctcc
     4081 gatgtcgtga agcaggccct gtcagggctg cagcagcaga gcgaccccga ttgcgaccag
     4141 caggtccaga accaatgcgg cggcggcttc agcgatttcg ccaccgagct ctgcaaggac
     4201 atcgtcaact acgccgcgca gcacatgggc ggcgagaagg ccaagggcaa gggcgccggc
     4261 agctggttcg aggcgctggc gcaggcgctc ggccaggcgc tggacaagca ggccgacaag
     4321 atcaagaacc tgtcctcgca ggtcaccgat gacaacgcga aggacaagcc gtcgacgatg
     4381 acccagctgc agtccgaatc gcagcgcatg agcttcatga tgagcgcagc cgatcaggtg
     4441 ctgaagacgc tgggcgaagc gctgtcgaac atggcgcgta agcagtaaga agccagggca
     4501 gttggacggg cgaaggcccg tccgctgccg gccgcttaga accaaacccc accaggaggc
     4561 cctatgtcat tacccatcga cattgtcggc tcgctgtggt tcatgccgac ggcgatctgg
     4621 gagtgggagc ccgaagcggg gcccgcgcct ggcagccagg gtgctgcagg ctgcgcggac
     4681 ggcggggcca ctggcggcga agccgtggca gaccagcact acgggatgcg gctcgtgttc
     4741 aagggctatg cgaacaccgc cagctttcat tggtttgccg ccgacagctt gctgtcgcgg
     4801 ccgtggaccc gagacgacgc cgacgacgcg catcgccggc gcgctacgga gcgaacgccg
     4861 agagtcgatg aagtaccgcg ggcggacgat gacgccaagt catcctgagc gccgctaggg
     4921 gcctggcggc tcgcggctaa ctgtcgcgca cttgcgggtc agcaggccgc cgggattagg
     4981 agcagtccta gctgggatgc tcggcaatgg cgggtcgcag cggcggccgc taggctcgat
     5041 gaagagagga gccgaccatg atccgtgttt ccgactacgt caatgtggcc gcctggactc
     5101 gctcgctgtc cgcgcctgcg cgcaccgaag ccaaggatcc gcgcagttgg ttcgaaggcg
     5161 acgccgccga cgtcgatgtg cgcatcgacg gcacgccgac ggcgcgcggc ctgaacatcg
     5221 agcagcatgc cggccgcgtg ctcgcctgcc tctgcggcga gcgcgacgct gccggctgaa
     5281 agcatggtga cgccatggcg gccctgctgt cggagccgaa ctgggtcggc gctgcccagg
     5341 cgctggtcga cggctgcgcc gacctgccca acgacgatga tcgtgtcgca ctcctcatcg
     5401 cggtgtgcca agggctgggc gacgagctat atcccgcttt catccgggtc ctttggacca
     5461 taggccggca tggcgaacac gcggccaagg ccgcagtcgc ctgtgcgctg gtgcatgcgc
     5521 tgcgcaccgg acggctgcct agcgggcgcc gcagcgcctg gggcgcgagt gcgccgatga
     5581 atgcgctcgg gagctacggc cgcacgcgct gcctcggccc gctggaatac ctgtgcgcat
     5641 ggcatgcgca gaccgaaccg gcatcggcgc tgacttcggc gcagttccac gtcgccgcgt
     5701 gcgcgctgat ggaactcatc gcctgcagtc gcgaagcgcg gctgctgtat tgcgagaagc
     5761 tgctaggcga cgtggacgac cccatcggcg gcgcgctggc gcgctacacg cgcgatgcct
     5821 tgcgcgcact ggccatcgca tggagcgaag gcgcaagcgc ttgggacgcc agcgcgcgct
     5881 tcatctgcac cctgcggcgc ccgagcccgg atgtgctggc cacactggcc gagcagtccg
     5941 caccggcatt gcgctgaacc acgacagccg acggcaacgc cggggctcaa caccgagcgg
     6001 tgctcgaaac tcccgccgat aggccagcgg cgacgtattg aacgccgcgc cgaagtgctg
     6061 gcgcaaggaa acggccgagc cgaagccggc ctcgctggcg acggcatcga tggagtggtc
     6121 ggtggtctcg agcatgcgct gcgcgcgcgc cgggcgctgg ggcattgggt cgggtgcgtg
     6181 aactcgacgc cgtaaggaga tgacattcag atggccctca gtgccgg
//
LOCUS       DASUCY010000028         2497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1366018, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000028 DASUCY010000000
VERSION     DASUCY010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1366018"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1281)
                     /locus_tag="VFV25_00585"
     CDS             complement(<1..1281)
                     /locus_tag="VFV25_00585"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008346433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY1391839.1"
                     /translation="MSSIDEDRTLIKPAPAERGNALPAGTRLAEFEILSVIGEGGFGI
                     VYLAQDHSLGRQVAIKEYMPAALAARTRALTVSVLSQRHAETFAAGLRSFINEARLLA
                     QFDHPALLKVYRFWEAHGTAYMVMPYYAGITLSQTLKRLKASPDEARLRALLHQLLDA
                     LELMHAARCYHRDISPDNILIQPDGRPLLLDFGAARRVIGDMTRALTVILKTGYAPVE
                     QYGEMPELKQGPWTDLYALASVVHYAIMGNTPPPSIQRYLNDKFVPLSRAAAGRYSPG
                     FLQAMDRALAVLPKDRPQSAAELDAMLGKADRSGPAVGPVRAAAGMVPHAAYVTGPAT
                     PVASSAEGLAARPQASPATAGVDILLAAPERAAAPAARQRLVGLMLGAAAVIALGVVA
                     LTYVRFERSSGPATASTATTSSESPTNATPTVDQL"
     gene            1547..>2497
                     /locus_tag="VFV25_00590"
     CDS             1547..>2497
                     /locus_tag="VFV25_00590"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY1391840.1"
                     /translation="MSQAENSKDQDPATSVEPSPVAYADDEATRIAPVVAPASAAAAG
                     AEPRTQPLNSQAASKATPSTPTAPAVPSRGARPETLPGGLAAGGPATRQVGRYQIQDR
                     LGRGGMATVFRAHDPGIGRDVAIKFLHATLCEDEEYRARFLREARAAGGLSHPNIVTV
                     YDVGEIEGRPYMAMELLEGEPLSEALEKGQSLPVRDVVVMGIQLARALDYAHARGIVH
                     RDIKPGNITRVKGTQTIKVLDFGIAHMDSAAGEQRTRVGDVLGTPQYMSPEQTQGEKL
                     DGRSDLFSAGIVLYQLLTGQRPFQADSLVALALKIAKEEPT"
BASE COUNT          398 a          836 c          878 g          385 t
ORIGIN      
        1 cagctggtcg acagtcgggg tcgcgttggt cggcgattcg gagcttgtcg ttgcagtcga
       61 tgcggttgct gggccgctgc tgcgctcgaa gcgcacatag gtcagggcca caacccccag
      121 cgcaatcacg gcggcggcgc ccagcatcag gccgacaagg cgctgtcggg cggcgggtgc
      181 ggcggcgcgc tcgggtgcgg ccaggagaat gtcgactccc gctgtcgccg ggcttgcttg
      241 cggccgggcg gcgaggcctt cggcactgct cgctacgggt gttgcggggc cggtgacgta
      301 ggcggcatgc ggcaccatgc ctgccgcggc gcgtaccggg cccacggccg gaccagatct
      361 gtccgccttg ccgagcatgg cgtccaactc ggccgcgctt tgcggacgat ccttgggcag
      421 caccgccagc gcgcgatcca tcgcctgaag gaagcccggg ctgtagcggc ctgccgcggc
      481 acggctcagc ggcacgaact tgtcgttcag atagcgctgg atcgaaggcg gcggtgtgtt
      541 gcccatgatg gcgtagtgca ctaccgaggc cagcgcgtag agatcggtcc aggggccctg
      601 cttcagctcg ggcatctcgc cgtattgctc caccggtgca tagccggtct tcagaatcac
      661 ggtgagcgcg cgcgtcatgt cgccgatcac gcggcgtgca gcgccaaagt ccagcagcag
      721 cggccgtccg tccggctgga tcaggatgtt gtcgggcgag atgtcgcggt gatagcagcg
      781 cgccgcgtgc atcagctcca gcgcgtccag cagctggtgc agcagggctc tcagccgggc
      841 ttcgtcgggg ctggccttta gacgcttgag cgtctggctc agcgtgatgc cggcgtagta
      901 gggcatcacc atgtaggcgg tgccgtgggc ttcccagaag cggtagacct tgagcagcgc
      961 cgggtggtcg aactgggcca gtagcctcgc ttcgttgatg aagctgcgca ggcccgcggc
     1021 gaaggtctca gcatggcgct gcgaaagcac cgataccgtc agcgcccggg tgcgcgcagc
     1081 cagcgcggcg ggcatgtatt ccttgatcgc cacctgccgc cccagcgaat ggtcttgcgc
     1141 gaggtagacg atgccgaagc cgccctcgcc tatgacggac aggatctcga attcagcgag
     1201 ccgggtgccg gcgggcaggg cattgccccg ttccgcaggc gcgggcttga tcagggtgcg
     1261 atcctcgtcg atggagctca tgtttgcgca ctggaccaac tgcgcgcagg gatgccgggc
     1321 gcggcggaac actgcgtcaa cagtactcca aacctgttgc atcgccacat gaccttcgac
     1381 ccacaccaaa gagggatggg atcgcgccgt gaccgcggcg ccgggcatga ctttcgacca
     1441 ttgctctccc agtgagggcc gacgaccatg ccgagggcgc gagctgggcg ccgtgtcggg
     1501 ccttgccatc agggctcgtc cctccgggag cttgaaggcc gctgcaatgt cacaggcaga
     1561 gaattcgaag gatcaggatc ccgcgaccag tgtcgagccc tcgcctgtcg cttacgccga
     1621 cgacgaagct acccgtatcg cgccggtggt cgctcccgcg agcgcggccg cggccggcgc
     1681 cgagccgcgc acccagccgc tgaactccca ggctgcatcc aaggccaccc ctagcacgcc
     1741 gacagcaccc gctgtgccgt cacgcggcgc gcgacccgag accctgcctg gcggccttgc
     1801 tgccggaggg ccagcgacac gccaggtcgg gcgctatcag atccaggatc gcctgggtcg
     1861 cggcggcatg gccacggtct tcagggccca cgaccccggc atcggccgcg acgtggcgat
     1921 caagttcctg catgccacgc tgtgcgagga cgaggaatac cgtgcgcgct tcctgcgcga
     1981 ggcgcgcgcc gccggcgggc tctcgcaccc caacatcgtg accgtctacg acgtgggcga
     2041 gatcgagggc aggccctaca tggcgatgga gctgctcgaa ggcgagccgc tgagcgaggc
     2101 cctggagaag gggcagtcgc tcccggtacg cgacgtggtg gtgatgggca tacagctggc
     2161 gcgtgcgctg gactacgccc acgcccgcgg catcgtgcac cgcgacatca agcccggcaa
     2221 catcacgcgg gtcaagggca cgcagacgat caaggtgttg gacttcggta tcgcccacat
     2281 ggactcggcc gccggcgagc agcgcacccg cgtgggtgac gtgctgggca cgccgcaata
     2341 catgtcgccc gagcagaccc agggcgagaa gctggacggc cgctccgacc tgttctcggc
     2401 gggcatcgtg ctctaccagc tgctcaccgg gcagcggccc ttccaggccg acagcctcgt
     2461 ggccctggcc ctcaagatcg cgaaggaaga gccgacg
//
LOCUS       DASUCY010000029         2002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1317817, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000029 DASUCY010000000
VERSION     DASUCY010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1317817"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..917
                     /gene="ubiG"
                     /locus_tag="VFV25_00595"
     CDS             204..917
                     /gene="ubiG"
                     /locus_tag="VFV25_00595"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEY1391841.1"
                     /translation="MTTTAQTNVDPAELAKFSELAHRWWDVDSEFRPLHQINPLRLDW
                     IDRMAGLSAKRVLDVGCGGGILADAMARRGAQVLGIDLATKSLKVAQLHALEAGTPNV
                     EYREIAAEALATEQPSSFDALTCMEMLEHVPQPASIVRACAQLVKPGGWVFFSTIARN
                     PKAFLFAIVGAEYLLNLLPKGTHEYARFIKPSELAHHARDAGLDVVAMRGMQYNPLTR
                     RYWLSDDTSVNYLMACRRP"
     gene            914..1591
                     /gene="gph"
                     /locus_tag="VFV25_00600"
     CDS             914..1591
                     /gene="gph"
                     /locus_tag="VFV25_00600"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312449.1"
                     /note="PGP is an essential enzyme in the glycolate salvage
                     pathway in higher organisms (photorespiration in plants).
                     Phosphoglycolate results from the oxidase activity of
                     RubisCO in the Calvin cycle when concentrations of carbon
                     dioxide are low relative to oxygen. This enzyme is a
                     member of the Haloacid Dehalogenase (HAD) superfamily of
                     aspartate-nucleophile hydrolase enzymes (PF00702); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009853 - photorespiration [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEY1391842.1"
                     /translation="MTAVRFNGVRAVLFDLDGTLADTAPDLGGAVNRMRERRGMAALP
                     IARLRPLASAGARGMLGAGFSIAPSDTGYEALRDEFLAEYEAALDRDSRLFDGMADCL
                     AALRALGLPWGVVTNKAERFTLPVLRGLGLDATAAVVIAGDTTPHAKPHPAPLLEACA
                     RLGISAHHAVYVGDDLRDMQAARAAGMPGIAAAYGYLGEAPQLERWPTDAVISKPLDL
                     PPLLQPA"
     gene            1666..>2002
                     /locus_tag="VFV25_00605"
     CDS             1666..>2002
                     /locus_tag="VFV25_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1391843.1"
                     /translation="MKKIIERLLAVWQQPAQRRRLIRIGSVLAVLALAWTLVVGWWLP
                     GFLQPRIEAAASEALGAPLKIERLQISPWALEARVQGLRLGPEAAPWLRLAELRADIS
                     SESLWRLAPV"
BASE COUNT          291 a          716 c          672 g          323 t
ORIGIN      
        1 tgggggtcgc cgacccaggc aagtatccgc ccagcgccgc gatcgcgaag gttatgctgc
       61 tgcccttccg gcctcaagag ggcatcgcgc tgtgcgagag tcgcgcgaca caaccgcgct
      121 aggcgggagc cgagcgtgag gctcgcgacc gttcgcagcc ccccgcgcct cacagcgccc
      181 ggccctgggc taccatcgcg cgcatgacaa cgacggcgca aaccaatgtc gacccggccg
      241 agctggccaa attcagcgag ctcgcccacc gctggtggga cgtcgatagc gaattccgcc
      301 cgctgcacca gatcaatccg ctgcgcctgg actggataga tcgcatggcc ggcctttcgg
      361 ccaagcgcgt gctcgacgtg ggctgcggcg gcggcatcct ggccgatgcc atggctcgcc
      421 gcggtgcgca ggtgctgggc atagacctcg cgaccaaatc gctgaaggtg gctcagctgc
      481 atgcgctgga agccggcacg cccaacgtcg aataccgcga gatcgctgcc gaggcgctgg
      541 ccaccgagca gccgagcagc ttcgacgcgc tgacctgcat ggagatgctg gagcatgtgc
      601 cgcagccggc ttccatcgtt cgcgcctgcg cgcaactggt caagccgggc ggctgggtct
      661 tcttctcgac catcgcgcgc aatcccaagg cctttttgtt cgccatcgtc ggcgcagaat
      721 acctgctcaa tctgctgccc aagggcacgc atgaatatgc gcgcttcatc aagcccagcg
      781 agctggccca ccatgcccgc gacgcggggc tggacgtggt cgcgatgcgc ggcatgcaat
      841 acaacccgct gacgcgccgc tattggctgt cggacgacac cagcgtcaac tatctgatgg
      901 cctgccgcag accatgacgg ccgtgcgctt caacggcgtg cgcgccgtgc tcttcgatct
      961 cgacggcacg ctggccgata cggcgcccga cctcggcggc gctgtcaacc gcatgcgcga
     1021 gcggcgcggc atggcggcgt tgccgatagc gcggctgcgc ccgcttgctt cggcgggcgc
     1081 gcgcggcatg ctcggcgcgg gcttctcgat cgcgccgagc gacaccggct acgaggccct
     1141 gcgcgacgaa ttcctcgccg aatacgaagc cgcgctggat cgcgactcgc gcctcttcga
     1201 cggcatggcc gattgcctgg ccgccttgcg cgcgctgggt ctgccctggg gcgtggtcac
     1261 caacaaggcc gagcgcttca cgctgccggt actgcgcggg ctcgggctcg atgcaacggc
     1321 cgccgtcgtc atcgccggcg acaccacgcc gcatgccaag ccgcatccgg cgccgctgct
     1381 cgaggcctgt gcgcggctgg gcatctctgc ccaccatgcg gtgtatgtcg gcgacgatct
     1441 gcgcgacatg caagccgcgc gtgccgccgg catgccgggc atcgccgcgg cctacggcta
     1501 tctcggcgag gcgccgcagc tcgagcgctg gcccaccgac gcggtgattt cgaagccgct
     1561 cgatctgcca ccgctgctgc agccggcttg aggcacatcg ttgacctgcc cagcggctcg
     1621 caggcacact gaagcgagcc gtcctgcgct tttcgctctc ccaagatgaa gaagatcatc
     1681 gagcgcctgc ttgctgtctg gcagcagcct gcgcagcgcc gcaggctgat ccgtatcggc
     1741 agcgtgctgg ccgtgctcgc gcttgcctgg acgctggttg tcggctggtg gctgcccggc
     1801 ttcctgcagc cgcgcatcga agctgcggcc agcgaggcgc tcggcgcacc gctcaagatc
     1861 gagcgcttgc agatctcacc ctgggcgctt gaggcgcgtg tgcagggcct gcgcctgggg
     1921 cccgaggccg cgccctggct gcgcctggcc gagctgcgcg ccgatatctc cagcgagtcc
     1981 ttgtggcggc tggcgccggt gc
//
LOCUS       DASUCY010000030         4798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_386004, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000030 DASUCY010000000
VERSION     DASUCY010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386004"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1475
                     /locus_tag="VFV25_00610"
     CDS             <1..1475
                     /locus_tag="VFV25_00610"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY1391844.1"
                     /translation="TRLGDWTLANKFGHTSGEVPTYALFSGANPQTLGSYIASQVTAA
                     NGDPNVVAAAGRPATGGTATFTNGGGAITNMAMPVIVNGMWAVDKTLRSTSNETRLSK
                     EIAPGNNLTLGLYLAGYSSHDVWTLGQAQLMTVQPHARLIDVQLDNGVKVSRNGYVPP
                     PFSFDLDASYSGNTRAFFVADEWQLTKELRVDLGARREIQRITGTIANVSNGDLDGDP
                     TTLYNNNLAYFNGTNTLIDSNLARNSFTAGANYNFSRELSAFVRVNKGHRMPDFDVLR
                     SRSANETRDPVEDITQYEIGLKTVNKLYSAFLTAYQNRLTNSQTQQFTNAGNVVLRPN
                     SKATGLEFELALRPGGGFEIAATGNFQDAKYLDFQQYTGNRVERAPKFQARVTPSWQT
                     QTGIGRLRTFATYSHVGERFADQTNTLRLPAYHTLDAGAVLSLYNGVELRLTGTNLTN
                     TIGLTEGNFRVPGQGVGQDGVFLARPLFGRAYELSVGFQF"
     gene            1488..2702
                     /locus_tag="VFV25_00615"
     CDS             1488..2702
                     /locus_tag="VFV25_00615"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1391845.1"
                     /translation="MRIKLAIILTYMLFGALLNSVGAVNLLVVQTLGVPRAEAALLDA
                     WKDLPIAVTSFLVASLLPRLGLRRSMLLALVAVGLACLAMPVLSAFWAIKLLFATLGA
                     GFALVKVSTYSMIGLVTADRAGHARFTNLLEGLFMVGVLGGYLLFSQAAHAEGQGWLS
                     VYSLLAALAALACLLLIASPLDEAPIKPAGATTLREDLLAMAPLLRLGLVAVFLLSAF
                     LYVLVEQGLSTWLPSFNREVLHLPTAMSVGLAVIMPASTALGRLTCAAIIGRLGWFAV
                     VMACLAGIAVLILVALPLARHSAPLTDSIGWLNAPPAAYLLPATGLLLAPIYPAINSA
                     MLSALPRPQHAAMTGLIVVFSALGGSLGSFITGRLFAAFDGTTAFGLLLVPTAALALA
                     LTRLRRGLHSHV"
     gene            2695..4194
                     /gene="treF"
                     /locus_tag="VFV25_00620"
     CDS             2695..4194
                     /gene="treF"
                     /locus_tag="VFV25_00620"
                     /inference="COORDINATES: protein motif:HMM:NF009773.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HEY1391846.1"
                     /translation="MSEPTPHELYGELFVAVQMARVFGDGKSFVDARPKLAPAAIRAR
                     YEAERKQPGFSLTDFVFAHFALPDHAAPIGPQLGIREHILALWPRLVREHREPLEGDS
                     LLPLPRPYVVPGGRFRELYYWDSYFTMLGLVADGRRDLAEDTLADFVHLIRRYGFIPN
                     SSRSYMLSRSQPPFFFKMVELLHADDPAAGFAHHLQELRAEHAFWMEGENGLAPGQAH
                     RRVLRTADGALLNRYWDDRCTPREESWREDAETAAASGRPPEQVWRDLRAAAESGWDF
                     SSRWCVDPGDMASIATTSFAPVDLNALLGGLESAIASGAARSGDAATAAAFEARAATR
                     RRLLQTLFWRADVGYFADLNWRDPGNPRPLTAAGLVPLYLGAATQPQADATAAQVQRH
                     LLGANGLLTTALATDQQWDAHNGWAPLQWMAAQGLALYGHWTLARYIAERWCASVHRV
                     YLATGRLHEKYDVAQDRPGGGGEYLAQDGFGWTNGVFVAFKEGLLCPHT"
     gene            complement(4232..4621)
                     /locus_tag="VFV25_00625"
     CDS             complement(4232..4621)
                     /locus_tag="VFV25_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY1391847.1"
                     /translation="MSNTPIASWHQLVRSHDATGLDDLLAEDAVFYSPVVHTPQRGKA
                     ITKKYLAAAFVVFFNPSFRYVRELVGERDAVLEFEVEIEGISVNGVDMIRWNDAGRIT
                     EFKVMLRPLKAVNLIHQKMAAMLQAGA"
     gene            complement(4677..>4798)
                     /locus_tag="VFV25_00630"
     CDS             complement(4677..>4798)
                     /locus_tag="VFV25_00630"
                     /inference="COORDINATES: protein motif:HMM:NF013377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HEY1391848.1"
                     /translation="YDVAQDRPGGGGEYLAQDGFGWTNGVFVAFKEGLLRPRA"
BASE COUNT          742 a         1796 c         1497 g          763 t
ORIGIN      
        1 ataccaggct cggcgactgg accttggcca acaagttcgg ccacacgagc ggcgaggtgc
       61 cgacctatgc cctgttctcc ggtgccaacc cgcagaccct gggcagctac atcgcctcgc
      121 aggtcactgc cgcgaacggt gatccgaatg tcgtcgccgc ggccggcagg cccgcgaccg
      181 gcggcacggc gacattcacg aacggcggcg gcgcaatcac caacatggcc atgcccgtca
      241 tcgtcaacgg catgtgggcg gtcgacaaga cgctgcgctc gaccagcaac gagacgcgcc
      301 tgtcgaagga gatcgccccc ggcaacaacc tgaccctcgg cctctacctg gccggctaca
      361 gctcgcacga cgtctggacg ctcggccagg cccagttgat gacggtgcag ccccatgccc
      421 gcctcatcga cgtgcagctc gacaacggcg tcaaggtctc gcgcaacggc tacgtgccgc
      481 cgcctttctc gttcgacctc gatgcgagct acagcggcaa cacccgagcc ttcttcgtgg
      541 cggacgagtg gcagctgacg aaggagctgc gcgtggacct cggcgcccgc cgcgagatcc
      601 agcgcatcac cggcacgatc gccaacgtca gcaacggcga cctcgacggc gacccgacga
      661 ccctctacaa caacaacctc gcctacttca acgggaccaa cacgctgatc gacagcaatc
      721 tcgcccgcaa ctcgttcacg gccggcgcca actacaactt cagccgcgag ctgagtgcct
      781 tcgtgcgcgt gaacaagggg catcgcatgc ccgacttcga cgtgctgcgc agccgcagcg
      841 ccaacgagac cagagatccg gtcgaggaca tcacgcagta cgagatcggc ctgaagaccg
      901 tgaacaagct ctacagcgcc ttcctgacgg cctatcagaa ccggctcacg aactcgcaga
      961 cgcagcagtt cacgaacgcc ggcaatgtcg tgctgcgccc caacagcaag gcgaccggcc
     1021 tcgagttcga gctggcgctg cgccctggcg gcggcttcga gatcgcggcg accggcaact
     1081 tccaggacgc gaaatacctg gacttccagc agtacaccgg caaccgcgtc gagcgcgcgc
     1141 cgaaattcca ggcccgcgtg acgcccagct ggcaaacgca aacgggcatc ggtcgcctgc
     1201 gtaccttcgc cacctacagc cacgtcggcg agcgcttcgc ggaccagacc aacacgctca
     1261 ggctgccggc ctaccacacg ctcgacgcgg gcgcggtact gtcgctctac aacggagtgg
     1321 agctgcgcct gaccggcacc aacctgacca acaccatcgg cctgacggaa ggcaacttcc
     1381 gtgtgccggg ccagggcgtg ggccaggacg gcgtcttcct cgcgcggccg ctgttcggcc
     1441 gggcctatga gctgagcgtc ggcttccagt tctgacgacg tcgccgcatg cgcatcaagc
     1501 tcgccatcat cctgacctac atgctgttcg gcgcattgct gaacagcgta ggcgcggtga
     1561 acctgctcgt cgtgcagacg ctaggcgtgc cgcgcgccga ggctgcgctg ctggacgcat
     1621 ggaaggacct gccgatcgcc gtcacgtcct tcctcgtcgc ctcgctgctg ccgcgcctcg
     1681 ggctcaggcg atcgatgctg ctggcgctgg tggcggtggg cctggcctgc ctcgccatgc
     1741 cggtgctgtc ggcgttctgg gccatcaagc tgctgttcgc gacgctgggg gcgggctttg
     1801 cgctggtcaa ggtctcgacc tattccatga tcggcctggt caccgccgac cgggccggcc
     1861 atgcgcgctt caccaacctg ctcgaaggtc tcttcatggt cggcgtgctg ggcggctatc
     1921 tgctgttcag ccaggccgcc catgccgagg gccagggctg gttgtcggtc tactcgctgc
     1981 tcgccgcact ggcagcactc gcctgcctgc tgctgatcgc cagcccgctg gacgaggctc
     2041 ccatcaagcc cgccggcgct acgacactgc gcgaggacct gctcgcgatg gctccgctgc
     2101 tgcgcctcgg tctcgtcgcg gtcttcctgc tgtcggcttt cctctacgtg ctcgtcgagc
     2161 agggcctatc gacctggctg cccagcttca accgcgaggt gctgcacctg ccgaccgcga
     2221 tgagcgtggg cctcgccgtc atcatgccgg cgtcgacggc gctcgggcgg ctgacctgcg
     2281 cggccatcat cgggcggctg ggctggttcg ccgtcgtcat ggcctgtctc gccggcatcg
     2341 cggtgctcat cctcgtcgcg ctgccgctgg cgcggcacag cgcgccgctc accgacagca
     2401 taggctggct gaacgcaccg ccagccgcct atctgctgcc ggccacgggc ctgctgctcg
     2461 cgcccatcta cccggccatc aactcggcca tgctgagcgc actgccgcgc ccgcagcacg
     2521 cggccatgac cggcctcatc gtcgtcttct cggcactggg cggctcgctc ggcagcttca
     2581 tcaccggccg cctcttcgcc gccttcgacg gcaccaccgc cttcggcctt ctcctcgttc
     2641 ccactgccgc cctcgcgctt gcgctgaccc gcctgcgccg cggcctgcat tcccatgtct
     2701 gaacccaccc cccacgagct ctatggcgag ctgttcgtcg ccgtgcaaat ggcccgcgtc
     2761 ttcggcgacg gcaagagctt tgtcgacgct cgccccaagc tcgcgccggc cgccatccgc
     2821 gcccgctacg aggccgagcg caagcaaccc ggcttttcgc tgaccgactt cgtgttcgcc
     2881 cacttcgcgc tgcccgacca cgccgcgccg atcggcccgc agctcggcat ccgcgagcac
     2941 atactcgcgc tgtggccgcg gctggttcgc gagcaccgcg agccgctgga gggcgacagc
     3001 ctgctgccgc tgccgcgacc ctatgtcgtg ccgggcgggc gctttcgcga gctctactac
     3061 tgggactcct atttcacgat gctcggcctc gtcgccgacg gtcgccgcga cctggccgag
     3121 gacacgctcg ccgacttcgt gcacttgatc cggcgctacg gcttcatccc caacagcagc
     3181 cgcagctaca tgctgagccg ctcgcaaccg cccttcttct tcaagatggt cgagctgctg
     3241 cacgccgacg accccgccgc cggcttcgca catcacctgc aggaactgcg cgccgagcac
     3301 gcgttctgga tggaaggcga gaacggcctc gccccaggcc aggcccaccg ccgcgtgctg
     3361 cgcaccgccg acggcgcgct gctgaaccgc tactgggacg accggtgcac gccgcgcgag
     3421 gaatcctggc gcgaggatgc ggagacggca gcggccagcg ggcgtccgcc cgaacaggtc
     3481 tggcgcgacc tgcgcgccgc cgccgaaagc ggctgggact tcagcagccg ctggtgtgtc
     3541 gaccctggcg acatggccag catagcgacg acttccttcg cgccggtgga cctgaacgcg
     3601 ctgctcggcg gcctcgaaag cgcgatcgcc agcggcgccg cgcgctcggg cgacgcagcc
     3661 acggccgccg ccttcgaggc ccgcgccgcc acgcgccgcc ggctgctgca gacgctgttc
     3721 tggcgtgccg acgtgggcta cttcgccgac ctgaattggc gcgaccccgg caacccccgc
     3781 ccgctgaccg ccgccggcct cgtgcccctc tacctcggcg ccgcgaccca gccccaggcc
     3841 gatgcgacgg cagcccaggt gcagcgccat ctgctcggtg cgaacggcct gctgaccacg
     3901 gccctcgcga ccgaccagca gtgggacgcc cacaacggct gggcgccgct gcagtggatg
     3961 gccgcgcaag gattggccct ctacggccac tggaccttgg cgcgctacat cgccgagcgc
     4021 tggtgcgcca gcgtgcaccg cgtctatctc gccacgggcc gcctgcacga gaaatacgat
     4081 gtcgcccagg atcgccccgg cggcggcggc gagtatctgg cccaggacgg cttcggctgg
     4141 accaacggcg tcttcgttgc attcaaggag ggcctgctgt gcccgcacac ctgagcccgc
     4201 cgccggctca ggcgttccgc actacccgaa gctaggcgcc cgcctgcagc atcgccgcca
     4261 tcttctggtg gatcaggttc accgccttca gcgggcgcag catgaccttg aactccgtaa
     4321 tccggcccgc gtcgttccac ctgatcatgt cgacgccgtt gacgctgatg ccctcgatct
     4381 cgacctcgaa ttccagcacc gcatcgcgct cgcccaccag ctcgcgcaca tagcggaagg
     4441 acggattgaa gaacacgacg aaggccgccg ccaggtattt cttcgtgatg gccttgccgc
     4501 gctgcggcgt gtggacgacg ggcgagtaga agaccgcgtc ttcggccagc aggtcgtcca
     4561 aaccggtggc gtcgtgacta cggaccagtt ggtgccacga ggcgatgggt gtgttgctca
     4621 agatgtgctc ccgtacaaag ctctctcaat cgtcagtaga cgggccgccg cagggctcag
     4681 gcgcgcgggc gcaacaggcc ctccttgaac gcgacgaaga cgccgttggt ccagccgaag
     4741 ccgtcctggg ccaagtactc gccgccgccg ccggggcgat cctgggcgac gtcgtatt
//
LOCUS       DASUCY010000031         8735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_257481, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000031 DASUCY010000000
VERSION     DASUCY010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 8735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8735
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257481"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="VFV25_00635"
     CDS             <1..888
                     /locus_tag="VFV25_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEY1391849.1"
                     /translation="TTENPSFEVNSALLSRATVHVLQPLSDDDLRVMLERGRALLGAP
                     PLSEPAALRLIGYADGDARRLLNTFENIVRMVGDVAEIDEALLERSLGEQLRRYDKGG
                     EQFYDTISALHKSVRGSDPDAALYWFVRMIDGGVDPRYVSRRLVRMANEDIGLADPRA
                     LRLALDAAETYERLGSPEGELALAQAVVYLSVAPKSNAVYAAYNAVRAFVKQDGTRAV
                     PLHLRNAPTRLMKQLDYGRGYRYAHDEEGGFAAGERYLPDDMPPQRFYEPVPRGLEIR
                     IGERLAELRRLNEAGGKEK"
     gene            1183..2106
                     /locus_tag="VFV25_00640"
     CDS             1183..2106
                     /locus_tag="VFV25_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY1391850.1"
                     /translation="MDIFIQQIINGLVLGSVYALVALGYTMVYGIINLINFAHGEVLM
                     VGAMVSWTIVTGMADSGLPGWLLLLISLAAAIVVCAVLNFSIEKIAYRPLRNAPRLAP
                     LITAMGMSLLLQTLAMIIWKPNYKAYPSLLPTQPFFIGGAVINTTQILILIVTAVTLA
                     GLMYLVNHTKLGRAMRATAENPRVAGLMGVRPDMVISATFVIGAALAALAGVMYAANY
                     GSVQHTMGFLPGLKAFTAAVFGGIGNLPGAMVGGVLLGLIESIGAGYIGTVTGGVLGS
                     HYQDIFAFIVLIAMLTLRPQGLLGERVADRA"
     gene            2127..3209
                     /locus_tag="VFV25_00645"
     CDS             2127..3209
                     /locus_tag="VFV25_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY1391851.1"
                     /translation="MKNKLVTFLVAALLLVGTPLVAQYFGNAWVRILDIALLYVLLAL
                     GLNIVVGYAGLLDLGYIAFYAVGAYMFALLASPHLTDTFENIRALFQNGLHAPWWLVI
                     PLGAGLAGFAGALLGAPTLKLRGDYLAIVTLGFGEIVRVFINNLDAPVNITNGPKGVS
                     QIDAIHFFGLDLGKTHSLFGFDVPAVTNYYFLFLALVLLSVLICYRLERSRIGRAWMA
                     IREDEIAAKAMGISTRNMKLLAFAMGATFGGVSGSMFAAFQGFVSPESFSLQESIMVV
                     AMVVLGGLGHLPGVILGAFVLAALPEVLRYVAGPLQAMTGGRLDAAILRQLLIALAMI
                     IIMLVRPRGLWPTPEHGKAAPAAVAK"
     gene            3222..3998
                     /locus_tag="VFV25_00650"
     CDS             3222..3998
                     /locus_tag="VFV25_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY1391852.1"
                     /translation="MSDTVLKVSNVSKRFGGLQALSDVGITINRGQVYGLIGPNGAGK
                     TTFFNVITGLYTPDSGSFELESKPYKPQAVHEVAKAGIARTFQNIRLFAEMTALENVM
                     VGRHTRTQSGLFGAVFRSNGFKKEEAEIAKRSQELLDYVGIGKYTEYKARTLSYGDQR
                     RLEIARALATDPKLIALDEPAAGMNATEKVVLRELIDRIRKDGRTILLIEHDVKLVMG
                     LCDRVTVLDYGKQIAEGTPADVQRNEKVIEAYLGAGHKAH"
     gene            4029..4742
                     /locus_tag="VFV25_00655"
     CDS             4029..4742
                     /locus_tag="VFV25_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY1391853.1"
                     /translation="MSDIMLKVSGLKVAYGGIQAVKGISLEVRQGELVSLIGANGAGK
                     TTTLKAITGVQPVGEGSVEFMGKPIKGQGSWDLVRQGLVMVPEGRGTFARMSITENLQ
                     MGAFTRKDNEIDADIEKVFGIFPRLKERRSQLAGTMSGGEQQMLAMGRALMARPKVLL
                     MDEPSMGLSPIMVDKIFEVVDEIHRQGVTVLLVEQNASRALQLASRGYVMESGEITMG
                     GEAKALLSDPKVRAAYLGE"
     gene            4852..5424
                     /gene="greB"
                     /locus_tag="VFV25_00660"
     CDS             4852..5424
                     /gene="greB"
                     /locus_tag="VFV25_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEY1391854.1"
                     /translation="MNKAFTREPDAVDEAGDDGDVALPPLPAGTKNYMTPQGYQRLRA
                     ELMSLLDEERPKIVETVSWAAKNGDRSENGDYIYGKKRLREIDRRIRFLTKRLDIAEV
                     ADPSAHHGEDQVFFGATVTYANQDGEERTITIKGIDEADNLKGEVSWVAPVARALLKA
                     RVGDEVLLHTPGGLERLEILDVVYPAPAQS"
     gene            complement(5511..7808)
                     /locus_tag="VFV25_00665"
     CDS             complement(5511..7808)
                     /locus_tag="VFV25_00665"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEY1391855.1"
                     /translation="MSSSRSDTAIRSKKPPAPAAASAPAPGLSDAAAASFAALTHKLD
                     YLDEAEIKRVREAYKFADEAHLGQFRASGEPYITHPIAVAGLCADWKLDAQAIMAALM
                     HDAMEDCGITKTELIERFGAPTADLVDGLTKLDKLQFSTREESQAESFRKMLLAMARD
                     VRVILIKLADRLHNMRTMDAMVAAKRSRIARETLDIYAPIAHRLGLNQTYRELQELSF
                     RYLQPWRHQTLSKAIRSARGNRRDLVERLQHEVEKAFAEAKLKVEIYGREKTLYSIYH
                     KMHEKHLSFAQVSDIFGFRIVVQTLPQCYLALGVLHQLYKPVPGRFKDYIAIPKANGY
                     QSLHTTLVSPLGTAVEFQIRTALMHAVAEKGIAAHWLYKAGSGGLSHANAHEAQRLGT
                     MWLQSLLDIQDETRDAAEFLEHVKIDLFPEAIYVFTPRSKILALPRGATPVDFAYAIH
                     SDVGDHCVAAKVNGEPVALRTELRSGDVVEVITATTSRPNPAWLNFVRTGRARSKIRH
                     YLKNMEAEESLELGEKLLAQALRTEGLTLPAGDESSAGQDGALWQALIRWSANRSQRE
                     LLIDIGLGKKIAIIVAKRLAQLMTDLGAKPDALTLTMGRYGADDATPAQGVVSIDGSE
                     GATVQLANCCHPIPGDGIVGYLGRGEGLVVHTVECNVGKRLFQRDSERWIKVEWADEA
                     QQRSFETCVNVLVNNGKGVLAQVAQAVSAAEADITHIDMTEARGSHTAELRLLLAVRD
                     RLHLAEVLRTLKRSPAVLRASRVKP"
     gene            complement(7924..8127)
                     /gene="rpoZ"
                     /locus_tag="VFV25_00670"
     CDS             complement(7924..8127)
                     /gene="rpoZ"
                     /locus_tag="VFV25_00670"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEY1391856.1"
                     /translation="MARITVEDCLQNIPNRFQLVLAATYRARMLSQGHAPKIETKNKP
                     GVTALREIAAGQVGLEMLRKVPV"
     gene            complement(8157..>8735)
                     /gene="gmk"
                     /locus_tag="VFV25_00675"
     CDS             complement(8157..>8735)
                     /gene="gmk"
                     /locus_tag="VFV25_00675"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEY1391857.1"
                     /translation="GAGKSSLVKALLELDAKLMVSISHTTRAPRGQEQNGREYWFISE
                     PEFRDMVKAGAFFEWAEVHGHLYGTSRTAIQQRMAAGEDVILEIDWQGALQIKQLFPN
                     AVLIFILPPSWDELRLRLQRRGEDRPEVIEQRLLNAREEVAQARHFDFIVINALFETA
                     LFDLKAIVHAQRLKYSAQRRGKPAVFDALDLP"
BASE COUNT         1468 a         2806 c         2930 g         1531 t
ORIGIN      
        1 actaccgaga acccgtcctt cgaggtgaat tcggcactgc tgtcgcgcgc cacggtgcat
       61 gtgctgcagc cactgtccga cgacgacttg cgcgtgatgc tggagcgcgg ccgagccctg
      121 cttggcgcgc cgccgctcag cgaacccgca gcgctgcggc tcatcggcta tgccgacggc
      181 gatgcgcggc ggctgctcaa caccttcgag aacattgtcc gcatggtggg cgacgtggcc
      241 gagatcgacg aggcgctgct ggaacgctcg ctgggcgagc agctgcgccg ctacgacaag
      301 ggcggcgagc agttctacga caccatctcg gcgctgcaca agagcgtgcg cggctccgac
      361 ccggatgcgg ccctgtactg gttcgtgcgc atgatcgacg gcggcgtgga tccgcgctat
      421 gtctcgcgcc gcctggtgcg catggccaac gaagacatcg gcctggctga tccgcgcgcc
      481 ctgcgtctgg ccctggacgc ggccgagacc tatgagcgcc ttggctcgcc cgagggcgag
      541 ctggcgctcg cccaagctgt cgtttacctg tcagtggcgc ccaagagcaa cgcggtctat
      601 gcggcttaca acgcggtgcg cgcctttgtg aagcaggacg gcacccgggc ggtgcctctg
      661 cacctgcgca atgctcccac ccggctgatg aagcagctcg actatggccg cggctaccgc
      721 tatgcccatg acgaagaggg cggattcgcc gcaggcgagc gctatttgcc ggacgatatg
      781 ccgccgcagc gtttctacga accggtgccg cgggggctgg aaatacgcat aggcgagcgt
      841 cttgcagaac tgcgccgcct caacgaggca ggcggcaaag agaaatagat cctggcagga
      901 aactagtgaa attccctagg tttcatcgtt ttcctgggtt ctttcgcatc tatctgccaa
      961 cctagaatcg cgcccgttcc tgcctcgggc aggccagagc atcgagcccg ccacacccta
     1021 ggcgggcttt ttgctacccg cacaagccat gagccgccgc agcccagggc tggttagagt
     1081 gcaagcgcaa acccccgcca ctagggtatg caaggtcgga gcggttcgtc gtcggcaagg
     1141 cgcgcatgcc tgccgcatct gcaagaacag gagtcgggtg gtatggacat cttcatccaa
     1201 caaatcatca acggtctggt gctgggcagc gtctatgcgc tggtggccct gggctacacg
     1261 atggtctacg gcatcatcaa cctgatcaac ttcgctcacg gcgaggtgtt gatggtgggg
     1321 gccatggtca gctggaccat cgtgacgggg atggccgatt ccggcctgcc cggctggttg
     1381 ctgctgctga tctcgctggc tgcggccatc gtcgtctgcg cggtgctcaa cttcagcatc
     1441 gagaagatcg cctaccggcc cttgcgcaac gcgccgcgcc tggcgccgct gatcaccgcg
     1501 atgggcatgt cgctgctgct gcagaccctg gcgatgatca tctggaagcc caactacaag
     1561 gcctatccca gcctgttgcc gacgcagccc ttcttcatcg gcggtgccgt gatcaacacg
     1621 acgcagatcc tgatcctgat cgtcacggcg gtcacgctgg ccgggctgat gtacctggtc
     1681 aatcacacca agctcggccg cgcgatgcgc gccaccgccg agaacccgcg tgtggccggg
     1741 ctcatgggcg tcaggcccga catggtgatc tcggccacct tcgtgatcgg cgccgccctg
     1801 gccgccctgg ccggcgtgat gtatgcggcc aactatgggt cggtgcagca cacgatgggt
     1861 ttcctgcccg gcctcaaggc cttcacggct gcggtgttcg gcggcatcgg caatctgccc
     1921 ggagcgatgg tgggcggcgt gctgctgggc ctgatcgagt ccataggtgc gggctacatc
     1981 ggcaccgtca cgggcggcgt gctgggcagc cactaccagg acatcttcgc cttcatcgtg
     2041 ctgatcgcga tgctgacgct gcgcccgcaa ggcctgctcg gcgaacgcgt ggccgaccgc
     2101 gcgtgaacca gacgggaacc atcatgatga agaacaaact ggtcaccttc cttgtcgccg
     2161 cgctgctgct ggtgggcacg ccgctggtcg cacagtactt cggcaacgcc tgggtccgca
     2221 tcctcgacat tgccctgctc tacgtgctgc tggcgctggg cctgaacatc gtggtgggtt
     2281 atgccggcct gctggacctg ggctatatcg ccttctacgc ggtgggtgcc tacatgttcg
     2341 cgctgctggc gtcgccgcac ttgaccgaca ccttcgagaa cattcgcgcc ctctttcaga
     2401 acggtctgca cgcgccctgg tggctcgtca ttccgctagg ggccggtctt gccggcttcg
     2461 ccggcgcgct gctgggggcg ccgacgctga agctgcgggg cgactatctc gccatcgtga
     2521 cgctgggctt cggcgaaatc gtccgcgtct tcatcaacaa cctcgatgcg ccggtcaaca
     2581 tcacgaacgg ccccaagggc gtcagccaga tcgacgcgat ccatttcttc ggcctcgatc
     2641 tcggcaaaac gcacagcctg ttcggcttcg acgtgcctgc agtcaccaac tactacttcc
     2701 tcttcctcgc gctggtgctc ctgagcgtat tgatctgcta ccggctggag cgctcgcgca
     2761 tcggccgcgc ctggatggcg attcgcgagg acgaaatcgc cgccaaggcc atgggcatca
     2821 gcacgcgcaa catgaagctg ctggccttcg cgatgggggc gaccttcggc ggcgtgtcgg
     2881 gctcgatgtt cgcggccttc cagggcttcg tctcgcccga gtccttcagc ctgcaggagt
     2941 ccatcatggt ggtggcgatg gtcgtgctgg gcggcctggg ccatctgccg ggcgtgatcc
     3001 tcggcgcctt cgtgctggcg gccctgcccg aggtgctgcg ctacgtggcc ggccctctgc
     3061 aggccatgac cggaggccgg ctggacgcgg ccatcctgcg ccagctgctg atcgcgctgg
     3121 ccatgatcat catcatgttg gtgcgcccgc gcggcctgtg gcccacgccc gagcatggca
     3181 aggcggcccc tgcagctgtg gccaagtgag gagaagaaca aatgagcgat accgtcctca
     3241 aagtgtccaa cgtgtccaag cgcttcggcg gcttgcaggc gctcagcgac gtgggcatca
     3301 ccatcaaccg cggccaggtc tacgggctga tcggacccaa cggtgccggc aagaccacct
     3361 tcttcaacgt catcaccggg ctgtacacgc ccgattccgg cagctttgag ctggaaagca
     3421 agccctacaa gccgcaggcg gtgcacgagg tcgccaaggc cggcatcgcg cgtaccttcc
     3481 agaacatccg cctgttcgcc gaaatgacgg cgctcgagaa cgtgatggtc gggcggcaca
     3541 cgcgcacgca atccggcctc ttcggcgccg tgttccgcag caacggcttc aagaaggagg
     3601 aggccgagat cgcgaagcgt tcgcaggagc tgctcgacta tgtcggcatc ggcaagtaca
     3661 ccgagtacaa ggcgcgcacg ctgagctatg gcgaccagcg tcgcctggag atcgcgcgcg
     3721 cgctggccac cgaccccaag ctgatcgcgc tcgacgagcc ggctgccggc atgaacgcca
     3781 ccgagaaggt ggtgctgcgc gagctgatcg accgcatccg caaggacggc cgcaccatcc
     3841 tgctgatcga gcacgacgtg aagctggtga tgggcctgtg cgaccgcgtg accgtgctcg
     3901 actacggcaa gcagatcgcc gaagggacgc cggccgacgt gcagcgcaac gagaaggtga
     3961 tcgaggccta tctgggcgcc ggtcacaaag cccattgatc ggcccttgat cggagctgga
     4021 gaaagaaaat gtcagacatc atgctcaagg tcagtggcct caaggtcgcc tacggcggca
     4081 tccaggccgt caagggcatc tcgctggaag tccgccaggg cgaactggtg agcctgatcg
     4141 gtgccaacgg cgccggcaag accacgacgc tcaaggccat caccggggtg cagccggtgg
     4201 gcgaaggcag cgtggaattc atgggcaagc ccatcaaggg ccagggttcc tgggatctgg
     4261 tcaggcaggg cctggtgatg gtgcccgagg ggcgcggcac cttcgcgcgc atgagcatta
     4321 ccgagaacct ccagatgggc gccttcacac gcaaggacaa cgagatcgat gccgacatcg
     4381 agaaggtgtt cggcatcttc ccgcgcctga aggagcgtcg cagccagctc gccggcacca
     4441 tgtccggcgg cgagcaacag atgctggcca tgggccgcgc gctgatggcg cgccccaagg
     4501 tgctgctgat ggacgagcct tcgatgggtt tgtcgcccat catggtcgac aagattttcg
     4561 aagtcgtgga tgagatccac cggcagggcg tcaccgtcct gctggtggag caaaacgcca
     4621 gccgggcgct gcagctggcc agtcgcggct atgtgatgga atccggcgag atcacgatgg
     4681 gcggcgaggc caaggctctg ctcagcgatc ccaaggtgag ggcagcctat ctcggcgagt
     4741 aaccggcccg cctcgaccct accggaagcg cggcttgaag ccgcgctttg tgttttggcg
     4801 cgcgcacaat tggagccggg aatcaccgtt gcgcgacgca gctggcatcg catgaacaag
     4861 gctttcacga gagaaccgga cgccgtcgac gaggcgggcg acgatggcga tgtcgccctg
     4921 ccgccgctgc cggccggcac caagaactac atgacgccgc agggctacca gcgcctgcgc
     4981 gccgagctga tgagcctgct cgacgaggag cgccccaaga tcgtggagac cgtgtcctgg
     5041 gccgccaaga acggcgaccg ctcggagaac ggcgactaca tctatggcaa gaagcggctg
     5101 cgcgagatcg accggcgcat ccgcttcctg accaagcggc tggacatcgc ggaggtggcc
     5161 gacccatccg cgcaccacgg cgaggaccag gtcttcttcg gcgccacggt cacctacgcc
     5221 aaccaggacg gcgaggagcg caccatcacg atcaagggca tcgacgaggc cgacaacctc
     5281 aagggcgagg tgagctgggt cgcgccggtc gcgcgcgcgc tgctgaaggc acgcgtcggc
     5341 gacgaggtcc tgctgcacac gccgggtgga ttagagaggt tagagatcct ggatgtggtc
     5401 tacccggcac ctgcgcagtc gtgactcatg ctgaagcccc ctctcggagg gggctcgggg
     5461 gagcggtgca tacgggcgaa gccctgagcc tgcctgtgcc ggctcagagc tcagggcttg
     5521 acgcgcgaag cgcgcagcac ggcaggcgag cgcttcagtg tgcgcagcac ctcggccaag
     5581 tgcaggcggt cgcgcacggc gagcagcagc ctcagttcgg cggtatggct gcctctggcc
     5641 tcggtcatgt caatatgggt gatgtcggcc tcggccgcgc tcacggcctg ggccacctgc
     5701 gccaacacgc ccttgccgtt gttgaccagc acgtttacgc aggtctcgaa ggagcgctgc
     5761 tgcgcctcgt cggcccactc gaccttgatc cagcgctcgc tgtcgcgctg gaacagccgc
     5821 ttgccgacgt tgcattcgac ggtgtgcacg accagacctt caccgcggcc cagatagccg
     5881 acgatgccgt cgccaggtat cggatggcag caattggcga gctggacagt ggcgccttcc
     5941 gagccgtcga tggagaccac accctgcgcc ggcgtcgcgt cgtcggcgcc gtagcggccc
     6001 atggtgaggg tcagggcgtc gggcttggcg ccgagatcgg tcatcaactg ggcgaggcgc
     6061 ttggcgacga tgatcgcgat cttcttgccc agtccgatgt cgatcagcag ctcgcgttgc
     6121 gagcggttgg cgctccagcg gatcagcgcc tgccagagcg cgccgtcctg gccggcgctg
     6181 gactcgtcgc ccgcaggcaa ggtgagccct tcggtgcgca gcgcctgggc cagcagcttc
     6241 tcgccgagct ccagcgattc ctcggcctcc atgttcttca ggtagtgccg gatcttggag
     6301 cgcgcccggc cggtgcgcac gaagttgagc caggccggat tggggcggga ggtggtggct
     6361 gtgatgacct ccaccacgtc gcctgagcgc aactcggtgc gcagcgcaac cggctcgccg
     6421 ttgaccttag ccgcgacgca atggtcgccc acgtcggagt ggattgcata tgcgaagtcc
     6481 accggcgtgg cgccgcgcgg cagggccagg atcttgctgc gcggcgtgaa gacgtagatc
     6541 gcctcgggga agaggtcgat cttcacatgc tccaggaact cggccgcgtc tcgcgtctcg
     6601 tcctggatgt cgagcaggga ctgcagccac atcgtgccca ggcgctgggc ctcgtgggca
     6661 ttggcgtgcg acagcccgcc gctgccggcc ttgtagagcc agtgagcggc gatccccttc
     6721 tcggccacgg cgtgcatgag ggcggtgcgg atctggaatt ccaccgccgt gcccagcggg
     6781 ctcaccagcg tcgtgtgcag cgactgatag ccgttggcct tggggatggc gatgtagtcc
     6841 ttgaagcggc ccggcaccgg tttatagagc tgatgcagca cgcccagcgc gagatagcac
     6901 tggggcagcg tctgtaccac gatgcggaag ccgaagatgt cgctcacctg cgcgaagctc
     6961 aggtgcttct cgtgcatctt gtgatagatg gaatagagcg tcttctcgcg gccgtagatc
     7021 tcgaccttca gcttcgcctc ggcaaaggcc ttctccactt catgttgcag ccgctccacc
     7081 aggtcgcgtc ggttgccgcg cgccgagcgg atcgccttgc tcagcgtctg gtggcgccag
     7141 ggctggagat agcggaagga cagttcctgc agctcgcgat aggtctggtt caggccgagg
     7201 cggtgcgcga tcggcgcgta gatgtccaaa gtttcgcggg cgatgcggct gcgcttggct
     7261 gcgaccatcg cgtccatggt gcgcatgttg tgcagccggt cggccagctt gatcaggatg
     7321 acgcgcacgt cgcgcgccat cgccagcagc atcttgcgga aggactcggc ctgcgattcc
     7381 tcgcgggtgg agaactgcag cttgtccaac ttggtgaggc cgtccaccag gtcggccgtc
     7441 ggcgcgccga agcgctcgat cagctcggtc ttggtgatgc cgcagtcctc catcgcgtcg
     7501 tgcatcagcg cggccatgat ggcctgcgca tcgagcttcc agtcggcgca caggccggcg
     7561 accgcgatcg ggtgggtgat gtagggctcg ccgctggcgc ggaactggcc cagatgagct
     7621 tcgtcggcga acttgtaagc ctcgcggacg cgcttgatct cggcctcgtc gaggtagtcc
     7681 agcttgtgcg tgagcgctgc gaaggaggcg gccgcggcgt cgctcagtcc aggggcaggc
     7741 gcgctcgccg cggcgggcgc gggcggcttc ttgctgcgga tggcggtatc ggagcgcgaa
     7801 ctgctcatga aaccacttta tagcggatca aaacgcctcg tcgctggcag gctgacagca
     7861 aacaacggac gcagagacaa gaaagcccgc ctggggcggg cttttcagcg aaatttcagg
     7921 gtgtcagacc ggcaccttgc gcagcatctc caggccgacc tggcccgcgg caatctcgcg
     7981 cagggcagtc acgccgggct tgttcttggt ttcgatcttg ggggcatggc cctggctcag
     8041 catgcgggcg cggtaggtgg cagccagcac cagctggaag cgattgggga tgttctgcag
     8101 gcagtcttcg acggtgatac gggccatgcg cttttgctcc ggatagggga cggaccctaa
     8161 ggcaggtcca gggcgtcgaa cacggcaggc ttgccgcgac gctgagccga gtattttaac
     8221 cgctgtgcgt ggacgatggc cttgaggtcg aaaagggccg tttcgaacag ggcattgatc
     8281 acgatgaagt cgaaatgccg ggcctgggcc acttcctcgc gggcgttcag taggcgctgc
     8341 tcgatcacct cgggcctatc ctccccgcgg cgctgcaggc gcaggcgcag ttcgtcccag
     8401 ctcggcggca ggatgaagat cagcaccgca ttggggaaga gctgcttgat ctgcaaggcg
     8461 ccctgccagt cgatttccaa gatcacgtcc tcgccggccg ccatgcgctg ctggatggcg
     8521 gtgcgcgagg tcccgtagag atggccgtgc acctcggccc actcgaagaa ggcaccggcc
     8581 ttcaccatgt cgcggaactc aggctcgctg atgaaccagt attcgcggcc gttctgctcc
     8641 tggccgcgcg gcgcgcgcgt ggtgtgcgag atcgacacca tcagcttggc gtccagctcc
     8701 agcaaggcct tgaccaggct ggacttgccc gcccc
//
LOCUS       DASUCY010000032         2384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1108992, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000032 DASUCY010000000
VERSION     DASUCY010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108992"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="VFV25_00680"
     CDS             <1..413
                     /locus_tag="VFV25_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heat-shock protein"
                     /protein_id="HEY1391858.1"
                     /translation="EHHRRLMRVVHLRLGHELAARAEAAKIAVAGGADTHIDLGHVEP
                     ELQVRLGEAQAMDALEDDMARIVDAALATTTQAGVAASGVGALYFTGGSTGLRALTAR
                     LAAAFPAARAVHGDRFASVATGLGLRASRLFGGS"
     gene            524..937
                     /locus_tag="VFV25_00685"
     CDS             524..937
                     /locus_tag="VFV25_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY1391859.1"
                     /translation="MGPLLDKFGPLLGRILIAALFIPTGFSKITDFQGTMGYVSSVHL
                     PLPAVAVVVAIVIEFGGGIALLAGYKARWVALALALFTLLSAIFFHNFWASPPDQVMM
                     QTINFWKNVAIAGGLLFVASFGAGPMSIDSSVKPS"
     gene            1020..1616
                     /locus_tag="VFV25_00690"
     CDS             1020..1616
                     /locus_tag="VFV25_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEY1391860.1"
                     /translation="MSSADDAQPLARPASCTELFITFTLLALQGFGGVLAIAQHVLCE
                     QKRWLTKEQFLEMLSLGQVLPGPNVCNVSLMVGDRYFGTRGAFASLAGMMAVPTVVVM
                     TLTVVYTHFAAVPAVAGALKGMGAVAAGLIIGSALKLAEPLKHNRMGLPVCIALGALT
                     FVMIALLHWPLVWVLLGLGILACGYAWTRVGQVARQIP"
     gene            1613..2146
                     /locus_tag="VFV25_00695"
     CDS             1613..2146
                     /locus_tag="VFV25_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEY1391861.1"
                     /translation="MTALGWGGLLGLFGHFMLLSLLSIGGAITVAPDMHRLLVNEQAL
                     LTDAQFSSSIAVAQAAPGPNILFVAVMGYQAAGFAGAAATLLGIMLPSTTLAFAASRW
                     GHSRSDWRAVQAFKTGMAPITLALLVATGWLLAVPAGDWRNWLLSAVTALLVWRTRLH
                     LLWLIAMGAVLGAMGWV"
     gene            complement(2175..>2384)
                     /locus_tag="VFV25_00700"
     CDS             complement(2175..>2384)
                     /locus_tag="VFV25_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012216652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY1391862.1"
                     /translation="MAEEFKDRGITVNAVLPSIIDTPANRADMREADFSRWVSPAAFA
                     NVIVFLLSEAATAINGALIPVKGRV"
BASE COUNT          327 a          832 c          792 g          433 t
ORIGIN      
        1 ccgagcacca tcgccggctg atgcgcgtcg tccatctgcg cctggggcac gagctggcgg
       61 cgcgcgccga ggcggccaag atcgcggtcg ccggcggcgc cgacacgcac atcgatctcg
      121 gccatgtcga gcccgagctg caggtcaggc tcggcgaagc gcaggccatg gacgccctcg
      181 aagacgatat ggcgcgcatc gtcgatgccg cgctcgcgac gaccacgcag gcaggcgtgg
      241 cagcttcggg cgtgggcgcg ctctacttca cgggcggctc gaccggcctg cgcgccttga
      301 ccgcacgcct cgccgcggcc ttcccggccg cgcgcgctgt gcacggtgac cgcttcgcca
      361 gcgttgccac cgggctgggt ctccgcgcca gccggctgtt tggcggaagc tgacgcaacg
      421 cctgtgctgc ggaacggcca tcttttctcg cccggcacgc gatgctaggc tgccggcggc
      481 ctgctgcaag gccgcagccg tcaaagcatc acaaggagaa aacatgggac cgttgttgga
      541 caagttcggc ccgctactgg gccgcatcct gatcgccgcg cttttcatcc ccaccggctt
      601 cagcaagatc accgacttcc agggcacgat gggctatgtc agctccgtgc atctgccgct
      661 gccggccgtg gcggtggtcg tcgcaatcgt gatcgaattc ggcggcggca tagccctgct
      721 ggcgggctac aaggcgcgct gggtcgcact ggccctggcc ttgttcacgc tcctcagcgc
      781 gatcttcttc cacaacttct gggcctcgcc gccggaccag gtgatgatgc agaccatcaa
      841 tttctggaag aacgtcgcca tcgcgggcgg cctgctcttc gtggccagct ttggcgcagg
      901 cccgatgtcc atcgacagtt cggtcaagcc gtcctgatat ccctgcgcgc tctccgccag
      961 ggggcggtgc ctacaatgtc ggcaccgccc ctcttctttt gcagcgctag ccacgcctca
     1021 tgagctcagc agacgacgcc cagcctctcg ccagacccgc ctcctgcacc gagctcttca
     1081 tcaccttcac cctactggcc ctgcaaggct tcggcggcgt gctcgccatc gcgcagcacg
     1141 tgctatgcga gcagaagcgc tggctcacca aggagcagtt cctggagatg ctgtcgcttg
     1201 gccaggtgct gcccggcccc aacgtttgca atgtctcgct gatggtcggt gaccgctact
     1261 tcggcacacg cggcgccttt gcctcgctgg ccggcatgat ggccgtgccc acggtggtgg
     1321 tgatgacgct gaccgtcgtc tacacccact tcgccgccgt gcccgccgtg gccggcgcac
     1381 tcaagggcat gggcgcggtg gccgccggtc tcatcatcgg ctcggcgctc aagctggccg
     1441 agccgctgaa gcacaaccgc atgggcctgc cggtgtgcat cgccttgggc gccctcacct
     1501 tcgtgatgat cgcgctgctg cactggccgc tggtctgggt gctgctgggc ttgggaatcc
     1561 tggcctgcgg ctatgcgtgg acgcgcgtag gccaggtcgc gaggcagatc ccatgacggc
     1621 gctgggctgg ggaggtctgc tgggcctgtt cgggcacttc atgctgctgt cgctgctgtc
     1681 cataggcggc gcgatcacgg tggcgccgga catgcaccgg ctgctggtga acgagcaggc
     1741 gctgctcacc gatgcgcaat tcagctcgtc gatcgccgtc gcccaggccg cgccggggcc
     1801 caacatcctc ttcgtcgcgg tgatgggcta ccaggccgcc ggcttcgcag gcgccgccgc
     1861 cacgctgctg ggcatcatgc tgccctcgac cacgctggcc ttcgcggcct cgcgctgggg
     1921 ccactcgcgc tcggactggc gtgccgtgca ggccttcaag accggcatgg caccgatcac
     1981 gctggccctg ctggtcgcca ccggctggct gctcgccgtg ccggccggcg actggcgcaa
     2041 ctggctgctg agcgccgtga cggcgttgct ggtgtggcgc acgcgcttgc acctgctgtg
     2101 gctgatcgct atgggtgcgg tgctgggggc gatgggctgg gtgtagctgg cagcgtaaaa
     2161 gcagtgcgcc tcgatcagac ccgccccttc acgggaatga gcgctccgtt gatcgcagtc
     2221 gccgcctccg agagcaggaa cacgatcaca ttcgcgaacg ctgccgggct gacccagcgg
     2281 ctgaagtcgg cctcgcgcat gtctgcacga ttcgcaggcg tatcgatgat gctgggcagc
     2341 acggcgttga ccgtgatgcc gcggtccttg aattcctcgg ccat
//
LOCUS       DASUCY010000033         2964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1044721, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000033 DASUCY010000000
VERSION     DASUCY010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044721"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VFV25_00705"
     CDS             complement(<1..364)
                     /locus_tag="VFV25_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY1391863.1"
                     /translation="MSALADTDAVIEARGLIKRFGDKTAVDKLDMTVRRGEIFGFLGP
                     NGSGKTTSIRMLCGLLTPDGGTGRTLGHDIVKEAGLIKRQVGYMTQRFGLYDDLTIRE
                     NLDFIGRMYGMQARRERVV"
     gene            complement(361..1350)
                     /locus_tag="VFV25_00710"
     CDS             complement(361..1350)
                     /locus_tag="VFV25_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEY1391864.1"
                     /translation="MRLRRCGRGWAWIVASLLVLAMGSACSPAADPRRYNGYVEAEYV
                     RVASPMAGRLTSLSVARGDEVKPQQPLFTLEQESEIAAVQGAQASLRRADAQAADLAK
                     GKRPDELAALAAALEAARAAARQSASEYDRQVQLARDGFISGANLDTLKAKRDADAAQ
                     VREAQAQWRVGKLAARPDARAAAAAEAAAAKADLQRSQWQLEQKTVTAQAAARVDDTL
                     YRVGEWVPAGSPVVSLVEPGGIKVRFFVPEDVVGSLRPGDAVSLRCDGCAAPVAAQIR
                     FISSTAEFTPPVIYSREQRSKLVFLVEARPAAADAFKLRPGQPVEVTLERKDS"
     gene            complement(1476..2816)
                     /locus_tag="VFV25_00715"
     CDS             complement(1476..2816)
                     /locus_tag="VFV25_00715"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY1391865.1"
                     /translation="MTTTISPSSFEALGLAAPLLRAVADLGFEQPTAVQAEAIPAALK
                     GGDWMVSSQTGSGKTAAFLLPVLHAILAQGLPERPARQGKGGYAPRQAVAPLAVVLCP
                     TRELAQQVAADAIDLVRGSRGLRVATVVGGMPFGKQLADLQGASLVVATPGRLLDLSE
                     RRQIRLDAVQVLVVDEADRMLDLGFAEDLEAIHQQCAQRSQTLMFSATFAPRIMKLAT
                     QVMREPGRLELATAQDVHTSITQTLHWADSTAHKRKLLDHWLRDAALEQAVVFASTQV
                     DADAIAEDLQQQGHAAVALHGAMPQSVRNRRLQNLRDGHIRILVATDVAARGIDVPSI
                     SHVINFGLPMKAEDYVHRIGRTGRAGRSGQAITLAEHRERHKIRAIEQFTRQPIAAST
                     IAGLEPTPPKPRPAHEPRFNARPGQRPGRDGGRPKHGPQRGGPKFGPRGAGAAR"
BASE COUNT          415 a         1019 c         1061 g          469 t
ORIGIN      
        1 ggacgacgcg ctcacgcctg gcctgcatgc cgtacatgcg gccgatgaag tcgaggttct
       61 cgcgtatcgt gaggtcgtcg tagaggccga agcgctgcgt catgtagccc acctggcgct
      121 tgatgagtcc ggcctccttg acgatgtcgt ggcccagcgt gcgcccggtg ccgccgtcgg
      181 gcgtgagcag gccgcacagc atgcggatcg aggtcgtttt gccgctgccg ttgggcccaa
      241 ggaagccgaa gatctcgccg cgccgcaccg tcatgtccag cttgtcgacc gcggtcttgt
      301 cgccgaagcg cttgatcagc ccgcgcgctt cgatgacggc atcggtgtcg gcaagtgcgc
      361 tcatgaatcc ttgcgctcca gcgtcacctc aaccggctgg ccgggccgca gcttgaaggc
      421 gtcggcggcg gccggcctcg cctcgaccag gaagaccagc ttgctgcgtt gttcacggct
      481 gtagatgacg ggcggcgtga actcggcggt gctcgagatg aagcggatct gcgcggctac
      541 gggcgcagcg catccgtcgc agcgcaggct caccgcatcg ccaggcctga ggctgccgac
      601 gacatcctcg ggcacgaaga agcgcacctt gatgccgccg ggctccacga ggctcacgac
      661 cgggctgccc gccggcaccc actcgccgac gcgatagagc gtgtcgtcca cgcgcgcggc
      721 cgcttgcgcc gtcacggtct tctgctccag ctgccattgg ctgcgctgca gatccgcctt
      781 ggccgccgcc gcctccgcag ccgcggcggc gcgcgcgtcg ggccgcgcgg cgagcttgcc
      841 cacccgccac tgcgcctgcg cctctcgcac ctgggcggcg tcggcatcgc gcttggcctt
      901 gagggtgtcg agattcgcgc ccgagatgaa gccgtcgcgt gccagctgca cctgccggtc
      961 gtattccgac gcggactggc gcgccgcggc gcgggccgcc tcgagcgcgg cggccagcgc
     1021 cgccagctcg tccggccgct tgcccttggc cagatccgcc gcctgcgcat cggcgcggcg
     1081 cagcgaggcc tgcgccccct gcaccgcggc gatctcgctt tcctgctcca gcgtgaagag
     1141 cggctgctgc ggcttcacct cgtcgccgcg ggccacggac aggctggtca ggcggcccgc
     1201 catcggcgag gccacgcgga cgtattcggc ctcgacatag ccgttgtagc gccgcgggtc
     1261 ggcagccggc gagcaggccg agcccatggc aaggaccagc agcgaagcca cgatccaagc
     1321 ccagccgcgc ccgcaccttc tcagccgcat cgatcgcctc cacatcgcac cgaactggcg
     1381 cgaggatgcc atacgcgccc cgctgcgagg agtgcgcggc atcaagaaag aaagaaggcc
     1441 gcgggaagcg gcctttttct ttctatctgc tggcttcagc gcgcggcgcc ggcgccacgc
     1501 gggccgaact tcggcccgcc gcgctgcgga ccgtgcttgg gacggccgcc atcgcgcccg
     1561 ggacgctgcc ctgggcgtgc attgaaacgc ggctcgtgcg ccggccgggg cttgggcggc
     1621 gtgggttcca ggcccgcgat ggtgctggcc gcgatcggct ggcgcgtgaa ctgctcgatg
     1681 gcgcggatct tgtggcgctc acgatgctcg gccagcgtga tggcctggcc gctacgcccg
     1741 gcgcggccgg tgcggccgat gcggtgcaca tagtcctcgg ccttcatcgg caggccgaag
     1801 ttgatgacat ggctgatgct gggcacgtcg atgccgcgcg ccgcgacgtc ggtggccacc
     1861 aggatgcgga tgtgcccgtc gcgcaggttc tgcagacggc ggttgcgcac gctctgcggc
     1921 atcgcgccgt gcagggcgac ggcggcgtgg ccttgctgct gcaggtcctc ggcgatcgcg
     1981 tcggcatcga cctgggtgga ggcgaacacc actgcctgct ccagcgctgc gtcacgcagc
     2041 caatggtcga gcagcttgcg cttgtgcgcg gtgctgtcgg cccagtgcag ggtctgggtg
     2101 atgctggtgt gcacgtcttg cgcggtagca agctcgaggc ggcccggctc gcgcatgact
     2161 tgcgtggcga gcttcatgat gcgcggcgcg aaagtggccg agaacatcag cgtctggctg
     2221 cgctgcgcgc actgctggtg gatggcttcc aggtcttcgg caaagcccag gtccagcatg
     2281 cgatcggcct cgtcgaccac cagaacctgc acggcgtcga ggcggatctg gcggcgctcg
     2341 ctcaggtcga gcaagcggcc gggcgtggca accaccaggc tcgcgccttg caggtcagcc
     2401 agttgcttgc cgaagggcat gccgcccacg acggtcgcga cgcgcaggcc gcgactgccg
     2461 cgcaccaggt cgatggcatc tgccgcaact tgctgggcca gctcacgcgt ggggcacagc
     2521 accacggcaa ggggcgccac ggcctggcgc ggtgcatagc cgcccttgcc ttggcgggcg
     2581 ggtcgctcgg gcaggccctg ggcaaggatc gcatgtagca cgggcagcag gaaggctgcg
     2641 gtcttgccgc tgccggtctg gctgctgacc atccagtcgc cgcccttcag ggccgccggg
     2701 atggcttcgg cctgcacggc cgtgggctgc tcgaagccca gatcggccac ggcccgcaag
     2761 aggggcgcgg caaggccgag ggcttcgaaa gaagaaggag aaattgtcgt tgtcatgcag
     2821 cagcagccgc ttttgtgcgg ctggtccgtc ttggggcggc ttggaatcga ggccccgttg
     2881 gtcaagaaaa catcgaccaa cagacggaat gcagcttgtg caatgcccgg ctcaagagcg
     2941 cccgggcggg actggtgacg atgc
//
LOCUS       DASUCY010000034         3455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1012620, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000034 DASUCY010000000
VERSION     DASUCY010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012620"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VFV25_00720"
     CDS             complement(<1..529)
                     /locus_tag="VFV25_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEY1391866.1"
                     /translation="MNIEQARFNMIEQQIRPWDVLDTSVLSLLAIVKREDFVPAAQRA
                     LAFMDLEVPLADGQVMLSPKVEARMLQELAPARHELVLEVGTGSGYMAALLAHKAQRV
                     VSLEIRPKLAAFARQNLQRAAVLNVEVREADGAKGYAAEAPFDAIVLSGSVAEVPQAL
                     LNQLKIGGRLIAIVGE"
     gene            complement(597..1298)
                     /locus_tag="VFV25_00725"
     CDS             complement(597..1298)
                     /locus_tag="VFV25_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY1391867.1"
                     /translation="MADISPPPTAQPASASTRQRRKEARPEQLLDAALSLFVEKGFAA
                     TRSEEVAARAGVAKGTLYLYYPSKEELLKAVVRENLSKCIGEAASIAEGYEGSSSELL
                     ALLLHTWWERVGEKPASGIFKIVLTEMGNFPDFAGFYLQEVIQPGRELFSRVLQRGID
                     AGEFRQIDLVQAVHVFIFPMLMLCMQKHSLGACPGMEEMTDAREFISTHIDVVLHGLQ
                     VREPQPATTAKSKRS"
     gene            1596..2432
                     /locus_tag="VFV25_00730"
     CDS             1596..2432
                     /locus_tag="VFV25_00730"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEY1391868.1"
                     /translation="MDLMWIKAALMGIVEGLTEFLPISSTGHLILAGSLLGFTEETVR
                     SAKLFEIVIQTGAIFAVIIIYWQRVRDTIAGLGSDAKARRFALNVAIAFLPAAVLGVL
                     FNKAIKAHLFTPLVVASSFIIGAFIILWAERRSAAAGEAMLQSVDDMTPMDSLKVGLA
                     QCLALVPGTSRSGATIIGGMLFGLSRKAATDFSFFLAIPTLIGAGVYSLWKERDQLSG
                     ADLPLLTIGTVVSFFVAWACVRWLLRYISSHDFKPFAWYRIAFGLIVLATWWGGWVDW
                     AG"
     gene            complement(2470..2967)
                     /locus_tag="VFV25_00735"
     CDS             complement(2470..2967)
                     /locus_tag="VFV25_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY1391869.1"
                     /translation="MNRRTAHLLLITPAAALAMPLLAQAADAPMGEATMLNANQLKWS
                     DAPPALPKGAKFTVLSGDPGKPGPYVLRVKMPANYRIAPHWHTQPENLTVLSGTFYLG
                     LGDKFDAKQAHALKAGGFHLLPGKTNHYAYTKSPTVLQVHAEGPFDITYLNPEDNPDK
                     SAAKN"
     gene            complement(3124..>3455)
                     /locus_tag="VFV25_00740"
     CDS             complement(3124..>3455)
                     /locus_tag="VFV25_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_038457415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEY1391870.1"
                     /translation="APASLAGVHVFFPDPWHKKRHHKRRLIQPGFVAELVTRLAPGGY
                     LHCATDWEPYAQQMLEVLGAEPALRNTAAGYAPKPAYRPLTKFEHRGLRLGHGVWDLV
                     FERRSVG"
BASE COUNT          561 a         1093 c         1174 g          627 t
ORIGIN      
        1 cctcgccgac gatggcgatc agccggccgc cgatcttgag ctggttgagc agcgcctgcg
       61 gcacctcggc taccgagccg gacagcacga tcgcatcgaa gggcgcctcg gccgcatagc
      121 ccttagctcc gtcggcctcg cgcacctcga cgttcagcac ggcggcgcgc tgcaggttct
      181 ggcgcgcgaa ggcagcgagc ttggggcgga tctccagcga cacgacgcgc tgcgccttgt
      241 gcgcgagcag cgcggccatg tagccgctgc cggtgccgac ttcgagcacc agttcatgcc
      301 gggccggggc cagttcctgc agcatccgcg cctcgacctt gggcgagagc atgacttggc
      361 catccgccag gggcacttcg agatccatga aggccagcgc gcgctgggcg gcaggcacga
      421 agtcctcgcg cttgacgatg gccagcagcg acagcaccga agtgtcgagc acgtcccagg
      481 ggcggatctg ctgctcgatc atgttgaagc gggcttgttc gatgttcatg acggcgttca
      541 ataggaaggc tgggtgcgaa gccggcgatt ctatgagggg cgcgtgacgg cgaggctcag
      601 ctgcgcttgc tcttggcggt ggtcgcaggc tgcggctcgc gcacctgcag gccatgcagc
      661 acgacatcga tatgggtgct gatgaactcg cgcgcgtcgg tcatttcctc catgccgggg
      721 caggcgccga gcgaatgctt ctgcatgcac agcatcagca tcgggaagat gaagacgtgc
      781 acggcctgga ccaggtcgat ctggcgaaac tcgccggcat cgatgccgcg ctggagcacg
      841 cggctgaaca gttcccgccc gggctggatc acttcctgga gatagaagcc tgcgaagtcg
      901 gggaagttgc ccatctcggt gagcacgatc ttgaagatgc ccgaggccgg cttttcgccg
      961 acgcgctccc accaggtgtg cagcagcagg gcgagcagct cagacgaaga gccctcgtag
     1021 ccctcggcga tcgaggcggc ctcgccgatg cacttgctca ggttctcgcg caccacggcc
     1081 ttgagcagct cctccttgct cgggtagtag aggtagagcg tgcccttggc aaccccggcg
     1141 cgcgctgcga cctcttccga gcgtgtcgcg gcgaagcctt tctcgacgaa gaggctcagg
     1201 gccgcgtcga gcaattgctc ggggcgcgct tccttgcgcc gctggcgcgt cgatgcgctg
     1261 gcgggctggg cggtcggggg agggctgata tcggccacgg cggagctgaa aataactgac
     1321 tgactggtca gtaatgtagc cgcatcggct ggaagggtca aggtcgctgg cctgcttgaa
     1381 gaggctctag ggcctggaat gttgagtatt ttgctgacta gatgctacaa atattgatgc
     1441 ataatttatt gaaatacagg gggtaatgcg agcattttgt caagaatcga ccgagccgct
     1501 cagactgggc taagcctatg cagccgcggg agccctaaga tgccgcgctt tgcttccggc
     1561 caagacgctg gcaaccagcg gcaaggagca tttctttgga cctcatgtgg atcaaggccg
     1621 ccctgatggg catcgtggaa gggctgaccg agttcctgcc gatctcgagc accggccacc
     1681 tcatccttgc cggctcgctg ctcggcttca ccgaggagac ggtgcgctcg gccaagctgt
     1741 tcgagatcgt gatccagacc ggcgcgatct tcgccgtcat catcatctac tggcagcgcg
     1801 tgcgcgacac catcgccggc ctgggcagcg atgcgaaggc gcgccgcttc gcgctcaatg
     1861 tggcgatcgc cttcctgcct gccgccgtgc tgggcgtgct gttcaacaag gcgatcaagg
     1921 cgcatctgtt cacgccgctg gtggtggcca gcagcttcat catcggtgcc ttcatcatcc
     1981 tgtgggccga gcggcgcagc gcagccgccg gcgaggccat gctgcaaagc gtggacgaca
     2041 tgacgcccat ggactcgctc aaggtcggtc tagcgcaatg cctggccctg gtgccgggca
     2101 ccagccgctc gggcgcgacc atcatcggcg gcatgctgtt cggcctgagc cgcaaggccg
     2161 cgaccgactt cagcttcttc ctcgcgatcc cgacgctgat cggcgccggc gtctacagcc
     2221 tgtggaagga gcgcgatcag ctatccggcg ccgacctgcc gctgctgacg ataggcaccg
     2281 tggtgtcctt cttcgttgcc tgggcctgcg tgcgctggct gctgcgctat atctccagcc
     2341 acgacttcaa gcccttcgcg tggtatcgca tcgccttcgg gctgatcgtg ctggccacct
     2401 ggtggggtgg ctgggtggac tgggccggct gaggcgcgat ggcggcggcc gcaccggcca
     2461 tgacatcgat tagttcttgg cggccgactt gtccgggttg tcctccggat tgagataggt
     2521 gatgtcgaag gggccttcgg cgtgcacctg cagcacggtc ggtgatttgg tgtaggcgta
     2581 gtgattggtc ttgcccggca gtaggtggaa gccgccggcc ttcaaggcat gggcctgctt
     2641 cgcatcgaat ttgtcgccca ggcccaggta gaaggtgccc gaaagcaccg tcaggttctc
     2701 gggctgcgtg tgccagtgcg gcgcgatgcg gtagttggcc ggcatcttga cccgcagcac
     2761 gtagggccca ggcttgcccg gatcgcccga gagcacggtg aactttgcac ccttcggcaa
     2821 cgcgggcggc gcatcgctcc atttcagctg gttcgcgttc aacatcgtcg cctcgcccat
     2881 gggcgcatcc gcagcctggg cgaggagcgg catggccagc gcagcggccg gcgtgatgag
     2941 cagcagatga gcggttcgac ggttcatgac ggctctcctc gaagtgcgcg acgctttgct
     3001 tgcagcgcac gggcggatgg cgtcgcgctg gccgagccgc ctgcgttcgc gcagtgtcgc
     3061 gctttcaggc gcggcgcgat agaccgaact gggccgaacc caccgccacc cgaccggcgt
     3121 gcgtcagccg accgagcgcc gctcgaatac gaggtcccac acgccatggc ccagcctcaa
     3181 gccacggtgc tcgaatttcg tgagcggccg gtaggccggc ttgggcgcat agcccgcggc
     3241 cgtgttgcgc agcgcaggct ccgcacccag cacttccagc atctgctgcg catagggctc
     3301 ccaatccgtc gcgcagtgca gatagccgcc cggggccagt cgagtgacca gctccgcgac
     3361 gaagccgggc tggatcaggc gacgcttgtg atggcgcttc ttgtgccagg gatcgggaaa
     3421 gaagacgtgc accccggcca gcgaggccgg cgcga
//
LOCUS       DASUCY010000035         2529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_96929, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000035 DASUCY010000000
VERSION     DASUCY010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96929"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /gene="guaA"
                     /locus_tag="VFV25_00745"
     CDS             <1..1009
                     /gene="guaA"
                     /locus_tag="VFV25_00745"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEY1391871.1"
                     /translation="VREICGTRADWIMGNYIEEAVARIREQVGDEEVILGLSGGVDSS
                     VAAALIHRAIGDQLTCVFVDHGLLRLNEGAMVMEMFAGKLHAKVVHVDASEQFLGHLK
                     GVTDPEQKRKIIGREFVEVFQREAKRLSNAKWLAQGTIYPDVIESGGAKTKKATTIKS
                     HHNVGGLPETLGLKLLEPLRELFKDEVRELGVELGLPPEMVYRHPFPGPGLGVRILGE
                     VKREYADLLRRADAIFIEELRNTRDGASGKSWYDLTSQAFAVFLPVKSVGVMGDGRTY
                     DYVVALRAVQTSDFMTADWAELPYALLKRVSGRIINEVRGINRVTYDVSSKPPATIEW
                     E"
     gene            1006..1608
                     /locus_tag="VFV25_00750"
     CDS             1006..1608
                     /locus_tag="VFV25_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HEY1391872.1"
                     /translation="MSSGRASGQLALTGLDGAPVPTDRLFFALYPDASAAARIAQLAQ
                     RLRGELRLRGRPLQTERFHVTLHHLGDYAGLPADVLRCAKQAAAAIAVPPFEIAFDRA
                     MSFDRKPSNRPFVLQGSGEGLAGVLAFQQALGEALKNAGLGRWVAPRFTPHVTLLYDD
                     RRVDEEAIEPIRWTVREFVLVDSLLGRTQHVPLERWPLGS"
     gene            1678..1917
                     /locus_tag="VFV25_00755"
     CDS             1678..1917
                     /locus_tag="VFV25_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1391873.1"
                     /translation="MRILFSVLSLLIVVAVIGLIAKKQLHALSAPSLPAVAASGAAPT
                     GNVRQQSQQMQQQVQQEMQRALQQGAQRAASDADQ"
     gene            1931..>2529
                     /gene="tadA"
                     /locus_tag="VFV25_00760"
     CDS             1931..>2529
                     /gene="tadA"
                     /locus_tag="VFV25_00760"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEY1391874.1"
                     /translation="MSEASPVSPADEYAMRIALDQAQNAWLVGEVPVGAVIMRQTPDG
                     PQVVATGYNRPITTHDPTAHAEIVALRHAAQLLENYRLPECELYVTLEPCAMCAMALM
                     HARFKRVVFGAPDPKTGAAGSVLDLFASSKLNHHTEVVGGVLAEACGQLLRDFFAERR
                     AQQRAEREAGGAGEPAASESSDEAIPVGQAAEVEAAHFDT"
BASE COUNT          420 a          849 c          869 g          391 t
ORIGIN      
        1 cgtgcgcgag atctgcggca cgcgcgccga ctggatcatg ggcaactaca tcgaggaggc
       61 ggtggcgcgc atccgcgagc aggtgggcga cgaggaggtg atcctcggcc tgtccggcgg
      121 cgtcgattcg agcgtggcgg ctgcgctgat ccatcgtgcc atcggcgacc agctcacctg
      181 cgtcttcgtc gaccacggcc tgctgcgcct gaacgaaggc gcgatggtga tggagatgtt
      241 cgccggcaag ctgcacgcca aggtggtgca tgtcgacgcc agcgaacagt tcctcggcca
      301 tctcaagggc gtcaccgatc ccgagcagaa gcgcaagatc atcggtcgcg aattcgtcga
      361 ggtgtttcag cgcgaggcga agaggctctc caatgcaaag tggctggcgc agggcacgat
      421 ctaccccgac gtcatcgagt ccggcggcgc caagactaag aaggccacca cgatcaagag
      481 ccaccacaac gtcggtggcc tgcccgagac gctggggctg aagctgctgg agcccttgcg
      541 cgagctcttt aaggacgagg tgcgcgagct cggcgtggaa ctcggcctgc cgcccgagat
      601 ggtgtatcgc catcccttcc cggggccggg gctgggcgta cgcatcctcg gcgaggtcaa
      661 gcgcgaatac gccgacctgc tgcgccgcgc cgatgcgatc ttcatcgagg agctgcgcaa
      721 tacgcgcgac ggcgcctcgg gcaagagctg gtacgacctc acgagtcagg ccttcgcggt
      781 cttcctgccg gtcaagagcg tgggcgtgat gggcgacggc cgcacttatg actacgtggt
      841 ggcgctgcgc gcggtgcaga ccagcgactt catgacggcc gactgggccg agctgcccta
      901 tgcgctgctt aagcgtgtgt ccggccgcat catcaacgag gtgcgcggca tcaaccgcgt
      961 gacctacgac gtctcgagca agccgccggc aacgatcgag tgggaatgag cagcggccgt
     1021 gcatctggcc agctggctct gaccggtctt gacggggcgc cggtgccgac cgatcggctg
     1081 ttcttcgcgc tctacccgga cgcaagcgcg gcggcgcgca tagcgcagtt ggcgcagcgc
     1141 ctgcgcggcg agctgaggct gcgcggccgg ccgctgcaga ccgaacgctt ccacgtcacc
     1201 ttgcatcatc tgggcgacta cgcgggcctg cctgcggacg ttctgcgctg cgccaagcag
     1261 gcggccgcgg ccatcgccgt gccgcctttc gagatcgctt tcgaccgggc gatgagcttc
     1321 gaccgcaagc ccagcaaccg gccttttgtg ctgcaaggca gcggcgaggg cctggcgggc
     1381 gtgttggcct tccagcaggc cctgggcgaa gcgctcaaga acgcggggct gggtcgctgg
     1441 gtggcgcctc gcttcacgcc gcatgtgacg ctgctgtacg acgatcgccg cgtggacgag
     1501 gaggcgatcg agcccatacg ctggacggtg cgcgaattcg tgctggtcga cagcctgctg
     1561 ggccgcaccc agcacgtgcc gctggagcgc tggcccttgg gctcttaagc gcttgagcgc
     1621 cgtcattggc gagccacagg gcagaatgca tctgccagcc catccatttg acgacccatg
     1681 cgcattctct tcagcgtcct gagcctgctg atcgtcgtgg ccgtgatcgg cctgatcgcc
     1741 aagaagcagc tgcatgcctt gagtgcgccc agcctgccgg ccgtggccgc ctcgggcgcg
     1801 gcgccgaccg gcaacgtgcg ccagcagtcg cagcagatgc aacagcaggt gcagcaggag
     1861 atgcagcgcg ccttgcagca aggcgcccag cgcgccgcct cggacgccga tcaatgaacg
     1921 catccgcgcc atgagcgaag cgagcccagt cagcccggcc gacgaatatg cgatgcgcat
     1981 cgcgctcgac caggcgcaga acgcctggct cgtcggcgag gtgccggtcg gcgcggtcat
     2041 catgcgccag acacccgacg gcccgcaggt ggtcgccacc ggctacaacc ggccgatcac
     2101 gacgcacgac ccgaccgctc acgccgagat cgtcgcgctg cgccacgcgg cccagctgct
     2161 ggagaactac cgcctgcccg aatgcgagct ctacgtgacg ctggagccct gcgcgatgtg
     2221 cgcgatggcg ctgatgcatg cgcgcttcaa gcgcgtcgtc ttcggcgccc cggatcccaa
     2281 gaccggcgcc gcgggctcgg tgctagacct attcgcaagc agcaagctca accatcacac
     2341 cgaagtcgtc ggcggcgtgc tggccgaggc ctgcggccag ttgctgcgcg atttcttcgc
     2401 ggagcgccgt gcccaacagc gcgccgagcg cgaggccggc ggggcgggtg agcccgcagc
     2461 ttccgaatcc tctgacgaag cgattcctgt ggggcaggcc gccgaagtcg aagcggcaca
     2521 tttcgacac
//
LOCUS       DASUCY010000036         2268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_209419, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000036 DASUCY010000000
VERSION     DASUCY010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209419"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..810)
                     /locus_tag="VFV25_00765"
     CDS             complement(178..810)
                     /locus_tag="VFV25_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY1391875.1"
                     /translation="MIKVILCDDHALIRRGIRDTLTDAPDMEVVGEAGDYGELRTLLR
                     SGKGCDVLVLDINLPGRSGLDVLHALKEENSPVRVLVVSMYPEDQYALRAMRAGAYGY
                     VNKGAEAGQIVAAVRTVAQGRKYVTPEMAQMLVENLTQPAPEQPHERLSDRELQTLVM
                     IASGKRLSDIAEELTLSPKTVSVYRARVLEKLGLSNNSELTVYAIRSGLV"
     gene            complement(807..1958)
                     /locus_tag="VFV25_00770"
     CDS             complement(807..1958)
                     /locus_tag="VFV25_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY1391876.1"
                     /translation="MRRLRVLHLEDSEFDHLMTLAHLERGGLDVEAVRVESEADYARA
                     LGQDWDVVLSDYNLPGFSGMAALKLLKDSGTLTPFIIVSGEIGEDVAVAAMRSGADDY
                     LLKNNLVRLVPAMLRAIEGAEMLRSHRLADAELAKSQQRLRELTHHLQTSVEAERAAI
                     AREIHDDVGGSLTAVKFDLAWIARHAENPAAAERAQQALETVTHAIEASQRIMHNLRP
                     AILEQGLVAALQWMTQRFEKRSGITTVFSSIGVVDTPHWPAGVALAAYRTAQEALTNV
                     SKHAQATRVQVGLAVTRGVLSLEMSDNGRGLSEEDLAKTHSFGIRGLHERANMVGGWV
                     DMSSGPDGTTLILSIPLRPAGKNSRHAPLDTMPAAIKDDLNDPTSWGSL"
BASE COUNT          339 a          779 c          752 g          398 t
ORIGIN      
        1 cggctttccc gtcacgctgg gcgtgggcct ggtcggcatg acgctgactc tgccagtgat
       61 gcaggcgccg ttcacgatgg cgttggagcg gatgctggga tatttccagt agttgcttag
      121 ctcgcgcttg cagccattga agatggcctc ggccaagccg atcggggcaa cggcccctca
      181 aaccaacccg ctgcgtatcg cgtaaaccgt caactccgag ttgttgctca accccagctt
      241 ctccagcacg cgggcgcggt acacgctcac tgtcttgggg ctgagcgtca gttcctcggc
      301 gatgtcggac aggcgcttgc ccgaggcgat catcaccagc gtctgcagct cgcggtccga
      361 cagtctctcg tgcggctgtt ccggcgccgg ctgggtgagg ttctcgacca gcatctgcgc
      421 catctcgggc gtgacgtact tgcggccctg ggccacggtg cgcacggctg ccacgatctg
      481 gccggcctcg gcacccttgt tgacatagcc gtaggcgccg gcgcgcatgg cgcgcagcgc
      541 gtactggtcc tccggataca tcgacaccac cagcacgcgc accggcgagt tctcttcctt
      601 cagcgcatgc agcacatcca ggccgctgcg gccgggcagg ttgatgtcca gcaccagcac
      661 gtcgcagccc ttgccgctgc gcagcagcgt gcgcagctcg ccgtagtcgc ccgcctcgcc
      721 gaccacttcc atgtccggcg catcggtcag cgtgtcgcgg atgccgcggc ggatcagcgc
      781 gtggtcgtcg cacaggatta ccttgatcac aggcttcccc aagatgtcgg atcgttcaaa
      841 tcatccttga tcgcggctgg catcgtatcg agcggcgcat gccggctgtt cttgccggcc
      901 ggccgcagcg gtatcgacag gatcagcgtg gtgccgtccg ggccgctgct catgtcgacc
      961 cagccgccca ccatgttggc gcgctcgtgc aggccgcgga tgccaaaaga gtgggtcttg
     1021 gccaggtctt cctcgctcaa cccacggccg ttgtcgctca tttccagcga cagcacgccg
     1081 cgcgtcaccg cgaggccgac ctggacgcgg gtggcctgcg catgcttgct gacgttggtg
     1141 agcgcttcct gtgccgtgcg gtaggcggcc aaggccacgc cggcgggcca gtgcggcgta
     1201 tccaccacgc cgatgctgga gaagacggtg gtgatgccgc tgcgcttctc gaagcgctgc
     1261 gtcatccact gcagtgctgc caccaggccc tgctcgagga tggctgggcg caggttgtgc
     1321 atgatgcgct ggctcgcctc gatggcgtgc gtgaccgtct ccagcgcctg ctgcgcacgc
     1381 tcggcagcgg ccgggttctc cgcatggcgg gcgatccagg ccaggtcgaa cttcaccgcc
     1441 gtcagcgagc cgccgacatc gtcgtggatc tcgcgcgcga tggcggcgcg ctccgcctcc
     1501 acgctggtct gcagatgatg ggtcaattcg cgcaggcgct gctgcgactt ggcgagctcc
     1561 gcgtcggcca gccggtgcga gcgcagcatc tcggcgcctt cgatggcgcg cagcatggcc
     1621 ggcaccagcc gcaccaggtt gttcttcagc aggtagtcgt ccgcgccgct gcgcatggcg
     1681 gccaccgcca cgtcctcgcc gatctcgccg gagacgatga tgaagggcgt gagcgtgccg
     1741 ctgtccttga gcagcttgag cgccgccatg cccgagaagc cgggcaggtt gtagtcggac
     1801 agcaccacgt cccagtcctg gccgagggcc cgggcgtagt cggcctcgga ctccacccgc
     1861 acggcctcca cgtccaggcc accgcgctcc agatgcgcga gcgtcatcag gtggtcgaac
     1921 tccgagtcct ccaggtgcag cacgcgcagg cggcgcatga agttcggggc ggtcgtggaa
     1981 gtggatgtca cgggcgcagc ttacggggag atcggcgtgg caaacaaccc gaactagtgg
     2041 aatagcaccg ccttttcccg cgtgatcgac tcaaggtcgg ggcgacatgt gccgaaaaat
     2101 tctagcaacc gcctgcctcg gcccggcctc ggccggcgcc ctgggcctac gcccccctgg
     2161 aacgacgatg ttgatgaacg atgtcccgcc cgatgacgac cgcgatggcg tcatcccgcc
     2221 gctgaaggcg accggcctgc aggagcagct gagcctggcc ggcgacga
//
LOCUS       DASUCY010000037         2071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_627156, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000037 DASUCY010000000
VERSION     DASUCY010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627156"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(275..1690)
                     /locus_tag="VFV25_00775"
     CDS             complement(275..1690)
                     /locus_tag="VFV25_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY1391877.1"
                     /translation="MSRKRTAWRTGSLALAALLALSGCTMIPKLERPAPPVAAQWPSA
                     QGAAGAVAATALPWQDYFADARLSKLIELALQNNRDLRVAVLSIEQVRAQYQIQRADL
                     LPTVSLAATGSRSPAPPAGSGNVYSFGLSLSSFEIDLFGRVRSLSEAAFAKYLATQEG
                     ARAAQISLVASVASTALAWQADQELLQLTQDTLKTREESLKLTQLKFDNGASSELDLR
                     QAQSLVENANAALAQQQRLLAVDVDALTLLVGQPLPADLVATSTPLADLHFASVPAGL
                     PSEVLVQRPDVREAERQLVAANADIGAARAAFFPRISLTASGGFASNALSSLFKHTAW
                     TASDQAVMPIFDYGRNSANLAVANTGRDIAVAQYEKAIQSAFREVADALAGEATLGEQ
                     LRAQQAQADAETARFKLVDLRYRNGASSYLELLDAQRSLFAAQQAAIQVRLAQLQNSV
                     ALYRSLGGGWSAAKSTDQAPS"
     gene            complement(1692..>2071)
                     /locus_tag="VFV25_00780"
     CDS             complement(1692..>2071)
                     /locus_tag="VFV25_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY1391878.1"
                     /translation="VLIIEFAKDLHAQGKSLVDAVLQAAHLRFRPILMTSMAFMLGVL
                     PLAVSSGAGSASQRAIGTGVMGGMFTATALAVFLVPVFFVVVRRLFKGSERQRKMYAH
                     QLDNNNGVGTGEVPPALPAGEGH"
BASE COUNT          319 a          696 c          736 g          320 t
ORIGIN      
        1 aacagggaaa tgcacagcac tctgcgtagc gaactcatgg tgtctccgtc cgttggttgc
       61 gtggcgtggt gcgaggcctt gcaaaaaagg gcggcgcctg tacgaggcgg gcaaggctcc
      121 ccggaagcgg caaatctagt cgccacgctg cagtgcggca ataggatttg catgtgttct
      181 tgcgcgcaga cccgcatgcg aggggtgggg cccccttggg acgaagccgg ccgccaggca
      241 ggcggccggg agccgatgtg tcagctcggt gacgtcagct cggtgcctgg tccgtcgact
      301 tggccgcgct ccagccgccg cccaggctgc gatacagcgc cacgctgttc tgcagctgtg
      361 ccagccgcac ctggatcgcg gcctgctggg cggcgaacag cgagcgctgg gcgtccagca
      421 gctccagata gctggaggcg ccgttgcgat agcgcaggtc gaccagcttg aagcgcgccg
      481 tctcggcatc ggcctgggcc tgctgagcgc gcaactgctc gcccagcgtg gcctcgccgg
      541 ccagcgcatc ggccacctcg cggaaggcgg actggatggc cttctcgtac tgcgccaccg
      601 cgatgtcgcg ccccgtattg gccacggcca ggttggcgct gttgcggccg tagtcgaaga
      661 tcggcatcac ggcctggtcg gaggcggtcc aggccgtgtg cttgaacagc gaggacaagg
      721 cattgctggc aaagccgccg cttgcagtca gcgagatgcg cggaaagaag gccgcgcggg
      781 cggcgccgat gtcggcattg gccgccacca gctgccgctc ggcttcgcgc acgtcgggcc
      841 gctgcacgag cacctcggaa ggcaggcccg cggggacgct cgcgaaatgc aggtccgcca
      901 gcggcgtgct tgttgccacc aagtcggccg gcagcggctg gccgaccagc agcgtcaagg
      961 cgtcgacatc gacagcgagc aggcgctgtt gctgggccag ggccgcgttc gcgttctcga
     1021 ccagcgactg cgcctgacgc aagtccagct cggagctggc gccgttgtcg aacttcagct
     1081 gggtgagctt cagcgactcc tcgcgggtct tgagcgtgtc ctgcgtcagc tgcagcagct
     1141 cctggtcggc ctgccaggcg agcgcggtac tggcgaccga ggccaccagc gagatctggg
     1201 ccgccctggc accttcctgc gtcgccaggt atttggcgaa ggccgcttcg ctcaggctgc
     1261 gcacgcggcc gaacaggtcg atctcaaagg agctgagcga caggccgaag ctgtagacat
     1321 tgccagagcc cgccggcggc gcgggcgagc ggctgccggt ggcggccagg ctcaccgtcg
     1381 gcagcaggtc ggcgcgctgg atctggtact gcgccctcac ctgctcgatg ctgagcaccg
     1441 ccacgcgcag gtcgcggttg ttctgcagcg cgagctcgat gagcttgctc aagcgcgcat
     1501 cggcgaagta gtcctgccag ggcaaggccg tcgcggccac tgccccggcc gcgccctggg
     1561 cagagggcca ttgtgcggcg acgggcggcg cgggccgctc cagcttgggg atcatggtgc
     1621 agccactcag cgccagcagt gcggcgagcg cgaggctgcc agtgcgccat gcggttcgtt
     1681 tcctgctcat ctcaatgccc ctccccggcc ggcagcgcgg gcggcacttc gcctgtaccc
     1741 acgccattgt tgttgtcgag ctggtgcgcg tacatcttgc gctgccgctc gctgcccttg
     1801 aagagcctgc gcacgaccac gaagaacacc ggcaccagga acacggccag cgcggtggcg
     1861 gtgaacatgc cgcccatcac gccggtgccg atggcgcgct ggctggccga gccggcgccg
     1921 ctggagaccg ccaacggcag cacgcccagc atgaaggcca tcgaggtcat caggatcggc
     1981 cggaagcgca gatgcgcagc ctgcagcacg gcgtcgacca ggctcttgcc ctgggcgtgc
     2041 aggtccttcg cgaactcgat gatcagcacc g
//
LOCUS       DASUCY010000038         2892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_161221, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000038 DASUCY010000000
VERSION     DASUCY010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161221"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="VFV25_00785"
     CDS             <1..610
                     /locus_tag="VFV25_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1391879.1"
                     /translation="IAVMRIAIGLKARTGRAVLVAVGGSVGEPLLVERSQLPLLPDGA
                     FAPYHAAQKLEALEAHKSVARSIAAAQELATSGIGDAARRLVAAGHDLRGCAVLVGTG
                     MPGWTTDEILAVHVRMHKAEGELFRDVLVSGVRACGLALATLPEKSALDDAARTLGLT
                     RTQLDARLIGLGKAAGPPWGKDQKEAAAAALVALAQASRERE"
     gene            complement(614..1546)
                     /locus_tag="VFV25_00790"
     CDS             complement(614..1546)
                     /locus_tag="VFV25_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY1391880.1"
                     /translation="MSIVTTESMHLIAPWPGGITLECRIAGPAGAPVLMFLHGFPEGA
                     FIWDELLAHFASRYRCVAPNLRGYAGSSAPQEVEAYRAKHLAADIAGLVQAVSPGAPL
                     AALVAHDWGGAVAWGLAAQKPGLMQRLVMINSPHPATFLRELTHNPAQQQASAYMNFL
                     CRPDAESLLAADDYARLWPFFENMGGKAWLDEELRRQYRAVWTRGVAEEASPGAGLRG
                     PLNYYRASPLRPASEPEAALHKVVFAPEQVSVKLPTTVIWGEADTALPPSLLDGLEAY
                     VPDLHVVRVPEATHWIVHERPQVVAREIEAALGR"
     gene            1677..>2892
                     /locus_tag="VFV25_00795"
     CDS             1677..>2892
                     /locus_tag="VFV25_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1391881.1"
                     /translation="MQQPTPPAGALAPAPAPAPAARPALPRLAFFGRVLWFCAFPLAT
                     VVLGNLVLFEVDQAKEAMEAYFDAVSFRLSHLASFLIAYWAWAVSTWYCARLLLGRRF
                     PVTAIGNCVGPVFAARLVKWLPRVLAVLSTLPTAIYLLVEGQWLVGVPVLVLALALLV
                     FVVWRRSWPWAGRRIAAAFSTDAALGEQWEHFDRIDPIGKALLWLLGLGPWGVLAGLL
                     LADPGFARTLGAPGLLLLALAAWTVFGSMVLIYWPLTRGWSAMVYVPLAMLLAFSFSN
                     DNHVIGQREGVTTPNAEARPDVAADFSHWLKAQAAAGRGKDPIYLVAMSGGASRAAYW
                     GAYTLGWLEDEARGAGRPFAADIYAISGVSGGSLGAAAFVTALASERAALQQQSGGHR
                     ATSLAGWMGELLG"
BASE COUNT          392 a          989 c         1049 g          462 t
ORIGIN      
        1 gatagcagtc atgcgcatcg cgataggact gaaggcacga accggccgcg cggtgctggt
       61 tgcggtcggc ggctcggtag gcgagccgct actcgttgaa cgatcgcagc tcccgctctt
      121 gccggacggt gcttttgcgc cttaccacgc agcccaaaag ctcgaggcgc tggaggctca
      181 caagagcgtc gcgcgcagca ttgcggcggc acaggagctt gcgacgagcg gcataggcga
      241 cgcggcacgg cgcctggtgg cggcagggca cgatctgcgc ggctgcgcag tgctggtcgg
      301 gaccggcatg cccggctgga ccaccgacga aatccttgcc gtccacgtcc gcatgcataa
      361 agccgagggc gagctgttcc gcgacgttct ggtctcgggc gtgcgcgcct gcggactggc
      421 gttggcaacg cttccggaaa agtcggcgct ggatgacgca gccaggacgc tcggcctgac
      481 ccgaacgcag ctcgacgcac gactcatcgg gctgggaaag gccgccggac ctccctgggg
      541 caaggaccag aaggaagcgg ccgctgcggc cctggtggcg ctcgctcagg cttcgcgcga
      601 gcgcgagtga gcctcagcgc cccaacgccg cctcgatctc gcgcgccacg acttgcggcc
      661 gctcgtggac gatccaatgc gtggcctcgg gcacgcgcac cacatgcagg tcaggcacat
      721 aggcctccag cccgtcgagc agcgaaggcg gcagcgccgt gtcggcctcg ccccagatca
      781 ccgtggtcgg cagcttcacg ctgacctgct cgggggcgaa cactaccttg tgcagggcgg
      841 cctcgggctc gcttgcgggg cgcagcggcg aagcgcggta gtagttgagc gggccgcgca
      901 ggccggcgcc gggcgaggct tcttcggcga cgccgcgcgt ccatacggcg cggtactgcc
      961 gcctcagctc ttcgtcgagc caggccttgc cgcccatgtt ctcgaagaag ggccacaggc
     1021 gtgcgtagtc gtccgcggcc agcaggctct cggcatcggg ccggcacagg aagttcatgt
     1081 aggcgctggc ctgctgctgg gccgggttgt gcgtcagctc gcgcaggaag gttgccgggt
     1141 gcggcgaatt gatcatcacc aggcgctgca tcagcccggg cttctgcgcg gccagccccc
     1201 aggccacggc gccgccccag tcgtgcgcca caagggccgc cagcggcgca cccgggctca
     1261 cggcctggac gagcccggcg atgtcggccg ccagatgctt ggcgcgatag gcctcgacct
     1321 cctgcggcgc cgacgagccg gcatagccgc gcaggttcgg ggcgacacag cgatagcgcg
     1381 acgcgaaatg cgcgagcagc tcgtcccaga tgaaggcccc ttccgggaag ccgtgcagga
     1441 acatcagcac cggcgcgccg gccgggccgg caatgcggca ttcgagcgtg atgccgccgg
     1501 gccagggcgc gatcaggtgc atggactcgg tagtgacgat gctcatgtgg agctccttgc
     1561 ctgcaggccg gtgcagtatg cgctggtgga gtccgcatcc gcgtcgcctg cgtgaccttg
     1621 ggcgaagaca aggcaccgcc ccaaggtgtc caatgcctcg ttcgggaggc atagccatgc
     1681 aacaacccac tccgccagcc ggcgcgcttg caccggcacc cgcgccggcc ccggccgctc
     1741 gacctgccct gccccgcctg gccttcttcg gccgggtgct gtggttctgc gcctttccgc
     1801 tggccaccgt tgtcctgggc aacctggtcc tgttcgaggt ggaccaggcc aaggaggcga
     1861 tggaggccta tttcgacgcc gtcagcttca ggctcagcca tctcgcgagc tttctgatcg
     1921 cctactgggc ctgggcagtg agcacctggt attgcgcgcg gctgctgctg gggcggcgct
     1981 ttccggtcac ggccatcggc aactgcgtgg ggccggtctt cgcggctcgc ctggtgaagt
     2041 ggctgccgcg cgtgcttgca gtgctctcca ccttgcccac cgcaatctat ctgctggtcg
     2101 aggggcagtg gctggtgggc gtgccggtgc tggtgctggc actggcgctg ctggtcttcg
     2161 tggtctggcg ccgcagctgg ccctgggccg ggcgtcgcat cgccgcggcc ttcagcaccg
     2221 atgcggccct gggcgagcaa tgggagcatt tcgaccgcat cgacccgatc ggcaaggccc
     2281 tgctgtggct gctcggcctc gggccctggg gcgtgctggc gggcttgctg ctggccgacc
     2341 ccggcttcgc ccgcacgctc ggcgcgccgg gcctgctgct gctggccttg gccgcctgga
     2401 cggtgttcgg cagcatggtg ctgatctact ggccgctgac gcggggctgg agcgcgatgg
     2461 tctatgtgcc gctcgcgatg ctccttgcct tctccttctc caacgacaac catgtcatcg
     2521 gccagcgcga gggcgtcact acgccgaacg ccgaggcccg ccccgacgtg gcggccgact
     2581 tctcgcactg gctcaaggcg caggcggcgg ccgggcgcgg caaggatccg atctatctgg
     2641 ttgcgatgtc gggcggcgcc tcgcgggcgg cctattgggg cgcctatacg ctgggctggc
     2701 tggaggacga ggcgcgcggc gcgggacggc ccttcgcggc cgacatctat gcgatcagcg
     2761 gcgtttcggg cggcagcctg ggcgcggccg ccttcgtcac ggcgctggcg agcgagcggg
     2821 cggcgctgca acagcaaagc ggcgggcacc gcgccacatc gctggccggc tggatgggcg
     2881 agctgctggg cc
//
LOCUS       DASUCY010000039         2446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_16663, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000039 DASUCY010000000
VERSION     DASUCY010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16663"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VFV25_00800"
     CDS             <1..445
                     /locus_tag="VFV25_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011003335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY1391882.1"
                     /translation="LLSFALMAVLLLVLKRTQLGRALRAIAESPRAAWLLGINVEGLF
                     MLTSFVAAALGGLAGVLIGLYSNAVFPLMGQPMLHKGIAVIILGGMGDVRGALLGGLF
                     LGFAEVLSVAYIGSTMRDAVAFGLLFLILLVRPTGLFGKVQERKA"
     gene            539..1390
                     /locus_tag="VFV25_00805"
     CDS             539..1390
                     /locus_tag="VFV25_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY1391883.1"
                     /translation="MEWFDGFWSVYSNLVLSLGTNALLALSIYLTLSCGMLAMANAAF
                     MGIGAYSASLLTMNAGAPFPLALLGGMAAPSVIAFLIGKPTLRLSGVYLAMATLALGE
                     VVRVLILNTETWTGGALGLNGIPQETLWWHVLLAVVLVLLLLWRLRRSKVGRAFEAIK
                     EDETAAALMGVDVAGHKMLAFVLGAAIAGLAGALNAHLTFFIGPNEYGFDRGVEILTM
                     AILGGVNGLTGPVLGAVILTLLPELLRSLADFRLVVNGLILAVVTLFLPKGLWEPARW
                     RRLLRRT"
     gene            1404..2186
                     /locus_tag="VFV25_00810"
     CDS             1404..2186
                     /locus_tag="VFV25_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY1391884.1"
                     /translation="MLKLESVAMRFGGLQVLQDVNLEVPGGSIFGLIGPNGAGKTTVF
                     NLITGLLQPSGGAISFDGKPLARLKPHEITRQGIARTFQNIRVFKEMSLLDNVVVGVH
                     RHLDYGAAGLLLATPGFRARERAARERARELLSWVRLDHKADETADSLSYGEQRKLEL
                     ARALATEPKLLLLDEPVAGMNASEKTELMREIENIRQRGYSIFLIEHDMRFVMGLCER
                     VAVLNFGRIICDATPDVVRNDPQVIEAYLGRDEDPLAVEAAA"
     gene            2183..>2446
                     /locus_tag="VFV25_00815"
     CDS             2183..>2446
                     /locus_tag="VFV25_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013209122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY1391885.1"
                     /translation="MTAPVLKVEGLQVAYGHIEAVKGISFEVRPGEITALVGANGAGK
                     STTLLALSGLVKPEAGRVIFEGEDVSRLKPHQRVERGIVQVAEG"
BASE COUNT          347 a          808 c          863 g          428 t
ORIGIN      
        1 cctgctgtcc ttcgcgctga tggcggtgct gctgctggtg ctcaagcgca cccagctcgg
       61 gcgcgcgctg cgcgccatcg ccgagtcgcc gcgcgcggcc tggctgctgg gcatcaacgt
      121 cgaaggcctg ttcatgctga cctccttcgt cgccgcggcg ctcggcgggc tggccggcgt
      181 gctgatcggc ctgtactcga acgcggtctt cccgctgatg ggccagccca tgctgcacaa
      241 gggcatcgcg gtcatcatcc tgggcggcat gggcgacgta cgcggcgcgc tgctcggcgg
      301 actcttcctc ggcttcgccg aggtgctgtc ggtggcctat atcggctcga ccatgcgcga
      361 cgccgtggcc ttcggcctgc tgttcctgat tctgctggtg cggcccacgg gcctgttcgg
      421 caaggtgcag gagcgcaagg catgagcccc gcgcggccgc ccgaaggggc gaactccctc
      481 ccggagggac ggcgcgcagc gcctaggggg cacacatgac cggggcgttg tcgtcatgat
      541 ggagtggttc gacggcttct ggtccgtcta cagcaacctg gtgctgagcc tgggcaccaa
      601 tgccctgctg gctctgtcca tctacctcac gctgtcctgc ggcatgctgg cgatggcgaa
      661 cgcggccttc atgggcatcg gtgcctacag cgcctcgctg ctgacgatga atgccggtgc
      721 gccttttccg ctcgcgttgc tgggcggcat ggcggcgccg tctgtcatcg ccttcctgat
      781 cggcaagccg acgctgcgcc tgtcgggcgt ctatctcgca atggcgacgc tggccctggg
      841 cgaggtggtg cgcgtgctca tcctcaacac cgagacatgg accggcggtg cgctgggcct
      901 caacggcatc ccgcaggaaa cgctgtggtg gcatgtgctg ctggccgtgg tgctggtgct
      961 gctgctgctg tggcggctgc gccgctccaa ggtcggccgc gccttcgaag cgataaagga
     1021 ggacgagacg gccgctgcgc tgatgggcgt ggacgtggcg gggcacaaga tgctggcctt
     1081 cgtactgggc gcggccatcg ccggcctggc gggtgcgctc aacgcccacc tgaccttttt
     1141 catcggcccc aacgagtacg gcttcgaccg cggcgtcgag atcctgacga tggcgatcct
     1201 gggcggcgtg aacggcctca caggcccggt gctgggtgcc gtgatcctca cgctcctgcc
     1261 cgaactgctg cgctcgctcg cggacttccg cctcgtcgtc aacggcctca tcctggccgt
     1321 cgtcacgctc ttcctgccca aagggctgtg ggagccggcg cgctggcggc gcctgcttcg
     1381 gcggacctga gaacaaccag atcatgctca agctcgaatc ggtggcgatg cgcttcggcg
     1441 gcctgcaggt gctgcaggac gtgaatctcg aagtgcccgg cggcagcatc ttcggcctca
     1501 tcggccccaa cggggctggc aagaccacgg tcttcaatct catcacgggc ctgttgcagc
     1561 cttccggtgg agcgatcagc ttcgacggca agccgctcgc caggcttaag ccgcatgaga
     1621 tcacgcgcca gggcatcgcg cgcaccttcc agaacatccg cgtgttcaag gagatgtccc
     1681 tgctggacaa cgtcgtggtc ggcgtgcacc gccatctgga ctacggagcg gccggcctgc
     1741 tgctcgccac gccgggcttc cgcgcgcgcg agcgtgcggc gcgcgaacgc gcccgcgagc
     1801 tgctgtcctg ggtcaggctg gaccacaagg ccgatgagac ggccgacagc ctctcctacg
     1861 gcgagcagcg caagctggag ctggctcgcg cgctggcgac cgagcccaag ctgctgctgc
     1921 tggacgagcc ggtggccggc atgaacgcga gcgagaagac cgagctgatg cgcgagatcg
     1981 agaacatccg ccagcgcggc tattcgatct ttttgatcga gcacgacatg cgcttcgtga
     2041 tgggcctgtg cgagcgcgtg gccgtgctca acttcggccg catcatctgc gacgcgacgc
     2101 cggacgtggt gcgcaacgat ccgcaggtga tcgaggccta cctgggtcgg gacgaagacc
     2161 cgctggcggt ggaggcggct gcatgacggc gcctgttctc aaggtcgagg gcctgcaggt
     2221 ggcctatggc cacatcgagg cggtcaaggg catcagcttc gaagtgcgtc cgggcgagat
     2281 cacggcgctg gtcggcgcaa acggggccgg caagtccacc acgctgctgg ccctgtcggg
     2341 gctggtgaag cctgaggccg gccgcgtgat cttcgagggc gaggacgtga gccggctcaa
     2401 gccgcaccag cgggtcgagc gaggcatcgt gcaggtggcc gagggc
//
LOCUS       DASUCY010000040         2199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_273730, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000040 DASUCY010000000
VERSION     DASUCY010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273730"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="VFV25_00820"
     CDS             complement(<1..905)
                     /locus_tag="VFV25_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY1391886.1"
                     /translation="MDMKTSPLSLLKDTSLLKTDALLGGEWIKGSSRFEVRDPATGLK
                     LAEVANLGAAETEAAIAAAGKAWPAWRAKTAKERAAVLMKWFALMHQHADDLARIMTA
                     EQGKPLAEARGEVTYGASFLEWFAEEAKRVYGETIPTTDNNKRYLVIKQPIGVCAAIT
                     PWNFPIAMITRKVAPALAAGCPVVIKPAEATPLSALAVAELAQRAGMPAGVLNIVTAD
                     AANSIAVGKVLCASEVVQHLSFTGSTEVGRILMQQCAPTVKKLSLELGGNAPFIVFDD
                     ADLDSAVEGAMVSKYRNAGQTCVCA"
     gene            1078..>2199
                     /gene="alaS"
                     /locus_tag="VFV25_00825"
     CDS             1078..>2199
                     /gene="alaS"
                     /locus_tag="VFV25_00825"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEY1391887.1"
                     /translation="MKVAEIRNTFLKFFESKGHAVVASSPLVPANDPTLMFTNSGMVQ
                     FKDVFLGTDKRPYVRAASVQACLRAGGKHNDLENVGYTARHHTFFEMLGNWSFGDYFK
                     RESLKWGWELLTEVYKLPKDKLWATVYIDDNEAYDIWTKEIGLPPERVVRIGDNKGAK
                     YASDNFWMMADTGPCGPCSEIFYDHGPDVAGGPPGSPDADGDRYIEIWNHVFMQFDMQ
                     PDGSVKKLPAPCVDTGMGLERLAAILQHVHSNYEIDLFAALIKAAARETHTQDLTNPS
                     LKVIADHIRATAFLVADGVIPSNEGRGYVQRRIIRRAIRHGYKLGQKTPFFHKLVPDL
                     VALMGEAYPNLAAHAQRIAEVLKAEEERFFETLANGMEIL"
BASE COUNT          369 a          754 c          715 g          361 t
ORIGIN      
        1 ttggcgcaca cgcaggtctg gccggcgttg cggtacttgc tgaccattgc gccttcgacg
       61 gcgctgtcca ggtcggcgtc gtcgaagacg atgaagggcg cattgccgcc cagctccagc
      121 gagagcttct tgaccgtggg cgcgcattgc tgcatcagga tgcggccgac ctcggtggag
      181 ccggtgaagg acaggtgctg cacgacctcg ctcgcgcaca gcaccttgcc cacggcgatc
      241 gagttcgcgg cgtctgccgt gacgatgttg agcacgccgg ccggcatgcc ggcgcgctgc
      301 gccagctcgg ccacggccag cgccgacagc ggcgtcgcct cggccggctt gatgaccacc
      361 gggcagcccg cggccagcgc cggtgcgacc ttgcgcgtga tcatcgcgat cggaaaattc
      421 cagggcgtga tggccgcgca cacgccgatg ggctgcttga tgacgaggta gcgcttgttg
      481 ttgtcggtgg tcgggatcgt ctcgccgtag acgcgcttgg cctcttcggc gaaccattcg
      541 agaaagctcg cgccataggt gacttcgccg cgtgcctcgg ccagcggctt gccctgctcg
      601 gccgtcatga tgcgggccag gtcgtcggcg tgctggtgca tcagcgcgaa ccacttcatc
      661 aggaccgcgg cacgctcctt ggccgtcttg gcgcgccagg cgggccaggc cttgccggcc
      721 gcggcgatgg cggcctcggt ctcggcggcg ccgagattgg ccacctcggc cagcttgagg
      781 ccggtggccg ggtcgcgcac ttcgaagcgg ctgctgccct tgatccattc gccgcccagc
      841 agcgcatcgg tcttgagcag gctagtgtcc ttgagcaggc tcaaggggga agtcttcatg
      901 tccatgacag gccttcgttt tcgggtcagc aagcatgata agccgcgggt tgcggcccgc
      961 gccgcgcccg ggtcttcgcc ccagggcgcg cagctacaat caaaagcttc gcgccatcaa
     1021 gtacttagcc ccgttttcgg gcgccgcgat gccgtcgcca gccagcagaa agtcccgatg
     1081 aaagtcgccg agattcgcaa caccttcctg aagttcttcg agtccaaggg ccacgccgtg
     1141 gtcgcctcca gcccgctggt gccggccaac gacccgacgc tgatgttcac caactcgggc
     1201 atggtgcagt tcaaggacgt cttcctcggc accgacaagc gcccctacgt gcgcgccgcc
     1261 tcggtgcagg cctgtttgcg cgccggcggc aagcacaacg acctggagaa cgtgggctat
     1321 accgcgcgcc accacacctt cttcgagatg ctgggcaact ggagcttcgg cgactacttc
     1381 aagcgcgaga gcctgaagtg gggctgggag ctgctgaccg aggtctacaa gctgcccaag
     1441 gacaagctct gggctacggt ctacatcgac gacaacgagg cctacgacat ttggacgaaa
     1501 gagatcggcc tgccgcccga gcgcgtggtg cgcatcggcg acaacaaggg cgccaagtac
     1561 gcctccgaca acttctggat gatggccgac accggcccct gcggcccctg ctcggaaatc
     1621 ttctacgacc acggccctga cgtggcgggc ggcccgccgg gcagccccga cgccgacggc
     1681 gatcgctaca tcgagatctg gaaccacgtg ttcatgcagt tcgacatgca gcccgacggc
     1741 agcgtgaaga agctgcccgc gccttgcgtg gacaccggca tgggcctgga gcgcctggcc
     1801 gcgatcctgc agcacgtgca cagcaactac gagatcgacc tgttcgccgc gctgatcaag
     1861 gcggccgcgc gcgagaccca tacgcaggac ctgaccaacc cctcgctcaa ggtcatcgcc
     1921 gaccacatcc gcgccaccgc cttcctggtg gccgacggcg tgatcccgag caacgagggc
     1981 cgcggctacg tgcagcgccg catcatccgc cgcgccatcc gccatggcta caagctgggt
     2041 cagaagacgc ccttcttcca caagctggtg cccgacctcg tggcgctgat gggcgaggcc
     2101 tacccgaacc tggcggccca tgcgcagcgc atcgccgagg tgctcaaggc cgaagaggag
     2161 cgcttcttcg agacgctggc aaacggcatg gagatcctc
//
LOCUS       DASUCY010000041         4316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1205541, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000041 DASUCY010000000
VERSION     DASUCY010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205541"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VFV25_00830"
     CDS             complement(<1..542)
                     /locus_tag="VFV25_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY1391888.1"
                     /translation="MAFVRAMLLAYERYGADPAEALKKAQITPAALRQPDAHITSAQM
                     VAFSAAAMQELDDEALGWFSRRLPWGSYGMLCRASITSPDLGVALKRWCRHHRLLTDD
                     ILLHLAVADGTATLHIEERLPLGTMREFCLVTLLRYVHGYACWAIDSQIPLREAGFPF
                     AAPPHHRVYPLMFPGPVYFEA"
     gene            758..2476
                     /locus_tag="VFV25_00835"
     CDS             758..2476
                     /locus_tag="VFV25_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018314435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY1391889.1"
                     /translation="MPQPASSLAPIGGLSQVQGAIDAPLSSLTVAGLLADTVGRYADR
                     PAVVFREQGVRWSWREFAAEVDAFASGLRVLGLKPGDRLGIWSPNRVEWLVTQFATAR
                     IGVILVNINPAYRLAELEYALNASGCRAIVSAERLKTSQYLEMLRKLAPELDAARPGE
                     LKAAKLPSLQFVIRMGDDSTPGMLNYRALLERGRAALDVSALVAVGKLLSCHDAINIQ
                     FTSGTTGNPKGATLTHHNVVNNARFIAQAMRFSEADSLCIPVPLYHCFGMVLAVLACV
                     STGAAMVFPGEAFDPAATLAAVAEEGCTALHGVPTMFIAELDHPQFASFDLSTLRTGI
                     MAGSPCPIETMKRVVSQMHMSEVTIAYGMTETSPVSFQSSTADPLERRVSTVGRIQPR
                     LDAKVVDVDGRVLPVGQTGELCVRGYSVMQGYWGDEARTREVVRDGWMHTGDLATIDP
                     EGYCNIVGRAKDMLIRGGENVYPREIEEFLFRHPKVQSVQVFGVPDPKYGEEVCAWIV
                     LKPGQQADEQEIRDFCRDQIAHYKVPRYIRFVDEMPMTITGKVQKFVMRQRMIDELKL
                     SVTKTA"
     gene            2509..3684
                     /locus_tag="VFV25_00840"
     CDS             2509..3684
                     /locus_tag="VFV25_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012733906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HEY1391890.1"
                     /translation="MSQNLGLSFDLGETIDALRDAVHDFASQEIAPRAAAIDRDNLFP
                     HDLWRKLGELGLHGMTVKEEYGGTELGYLAHIVAMEEVSRASASVGLSYGAHSNLCVN
                     QIHRNGSEAQKKKYLPKLVSGEHVGALAMSEPNAGSDVVSMKLRAEKKGDRYVLNGAK
                     MWITNGGDADTLVIYGKTEPELGARGMTAFIVEKGFKGLSFGNKLDKLGMRGSNTWPV
                     FFQDCEVPEENVLGGEGNGAKVLMSGLDYERTVLSGGPLGIMAACMDAVVPYIHERKQ
                     FGQSIGEFQLMQGKVADMYTTWQATRAYVYAVGKACDRGDHARTLRKDAAGAILYSAE
                     KATWMAGEAIQTLGGVGYTKEFPVERLWRDAKLYEIGAGTSEIRRMLIGRELFSETA"
     gene            3744..>4316
                     /locus_tag="VFV25_00845"
     CDS             3744..>4316
                     /locus_tag="VFV25_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEY1391891.1"
                     /translation="MPALASKLNPRSEDFKANAAVMRGLVDDLNAKLAQIAQGGGEAP
                     RAKHLARGKLLPRDRVEMLLDPGTPFLELAPLAALDVYKEKDGSDSAPGAGVVAGIGR
                     VNGVECMVVCNDATVKGGTYYPLTVKKHLRAQEIAEANRLPCIYLVDSGGANLPNQDE
                     VFPDRDHFGRIFYNQANLSAQGIPQIAVVMG"
BASE COUNT          755 a         1441 c         1462 g          658 t
ORIGIN      
        1 gcctcgaaat agaccgggcc cgggaacatc agcggataga cccggtgatg gggcggcgcg
       61 gcgaagggga agcccgcctc gcgcagcggg atctgcgagt cgatggccca gcaggcatag
      121 ccatgcacat agcgcagcag ggtgacgagg cagaactcgc gcatggtccc cagcggcagc
      181 ctttcctcga tgtgcagggt cgcggtgccg tcggcgaccg cgaggtgcag caggatgtca
      241 tcggtcagca ggcgatggtg gcggcaccag cgcttgagcg cgacccccag atcgggcgag
      301 gtgattgagg cgcggcacag catgccgtag ctgccccagg gcaggcggcg cgagaaccag
      361 cccagcgcct cgtcgtccag ttcctgcatc gccgcggccg agaaggccac catctgggcg
      421 gaggtgatgt gggcatccgg ctggcgcaaa gccgctggcg tgatttgtgc cttcttcagc
      481 gcctctgcgg ggtcggcgcc atagcgctcg taggccagca gcatggctct cacgaaggcc
      541 attggcgtgg ccgcctgccg agccttgggc ggcggcttgc gggggcgatc gggggcgcgg
      601 accggcatgc cgcgagtctg gctggtgctg gcaggatttg caacctcatt ggcgccaaat
      661 gcgacgcacg cgggcgtaat ctctgggcca aaacgtgggc gcgctgtggg ccgggcaggt
      721 cgcgagcgcc atgaacgccc ctagccggag acaaagcatg ccgcagccag cctctagtct
      781 cgcccccatc gggggactgt cccaggtgca gggcgcgatc gacgcgccgc tgtccagcct
      841 caccgttgcc ggcctgctgg ccgacacggt cgggcgctac gccgaccggc cggcggtcgt
      901 gttccgcgag cagggtgtgc gctggagctg gcgcgaattc gcggccgagg ttgatgcctt
      961 cgcgtccggc ctcagggtgc tgggcctcaa gcccggcgac cgcctgggca tctggtcgcc
     1021 aaaccgcgtc gaatggctgg tcacgcagtt cgccacggcg cgcatcggcg tcatcctcgt
     1081 caacatcaac ccggcatacc gcctggccga gctggaatac gcgctcaacg cctcgggctg
     1141 ccgcgcgatc gtgtcggccg agcggctcaa gacctcgcaa tacctcgaga tgctgcgcaa
     1201 gctcgcgccc gagctcgacg ccgcgcggcc gggcgagctg aaggcggcca agctgccgtc
     1261 gctgcagttc gtgatccgca tgggcgacga ttccacgccc ggcatgctga actaccgcgc
     1321 gctgctggaa cgcggccgcg cggcactcga tgtttcggcg ctcgttgccg taggcaagct
     1381 gctgagctgc cacgacgcga tcaacatcca gttcaccagc ggcaccaccg gcaaccccaa
     1441 gggcgcgacg ctgacccatc acaacgtggt caacaacgcg cgcttcatcg cccaggcgat
     1501 gcgcttttcc gaggccgact cgctgtgcat cccggtgccg ctgtaccact gcttcggcat
     1561 ggtgctggcg gtgctggcct gcgtatccac cggcgccgcg atggtcttcc ccggcgaggc
     1621 cttcgacccg gcggccacgc tggcggccgt ggccgaggag ggctgcacgg ccctgcacgg
     1681 cgtgccgacg atgttcatcg ccgagcttga ccacccgcag ttcgcgagct ttgacctctc
     1741 cacgctgcgc actggcatca tggccggctc gccctgcccc atcgagacga tgaagcgcgt
     1801 ggtctcgcag atgcacatga gcgaggtcac gatcgcctac ggcatgaccg agacctcgcc
     1861 ggtctccttc cagagctcga ccgccgaccc gctggagcgg cgcgtctcca cggtcggccg
     1921 catccagccg cggctggatg ccaaggtcgt cgacgtggac ggccgggtgc tgcccgtggg
     1981 ccagaccggg gagctgtgcg tgcgcggcta ttcggtgatg cagggctact ggggcgacga
     2041 ggcgcgcacc agggaggtgg tgcgcgatgg ctggatgcac accggcgacc tcgcgaccat
     2101 cgaccccgaa ggctattgca acatcgtcgg ccgcgccaag gacatgctga tccgcggcgg
     2161 cgagaacgtc tacccgcgcg agatcgagga attcctgttt cgccacccca aggtgcagtc
     2221 ggttcaggtc ttcggtgtgc ccgaccccaa gtacggcgag gaagtctgcg cctggatcgt
     2281 cctcaagccc ggccagcagg ccgacgagca ggagatccgc gatttctgcc gcgaccagat
     2341 cgcgcattac aaggtgccgc gctacattcg tttcgtcgac gagatgccga tgaccatcac
     2401 cggcaaggtg cagaagttcg tgatgcgcca gcgcatgatc gacgagctca agctgagcgt
     2461 taccaagact gcctgagcag ccaccagaca caagcaggag aacatcacat gtcccaaaac
     2521 ctcggcctga gcttcgatct cggcgagacc atcgatgcgc tgcgcgacgc ggtccatgat
     2581 ttcgccagcc aggaaatcgc gccgcgcgcc gccgccatcg accgcgacaa cctgttcccg
     2641 catgacctgt ggaggaagct cggcgagctt ggcctgcacg gcatgacggt caaggaggaa
     2701 tacggcggca ccgagctggg ctatctggcc cacatcgtcg cgatggagga ggtctcgcgc
     2761 gcctcggcct cggttggcct gtcctacggc gcgcactcca acctctgcgt caatcagatc
     2821 caccgcaacg gcagcgaggc gcagaagaag aagtacctgc ccaagctggt gagcggcgag
     2881 catgtcggcg cgctggccat gagcgagccc aatgccggct ccgacgtggt cagcatgaag
     2941 ctcagggccg agaagaaggg cgaccgctat gtgctcaacg gcgccaagat gtggatcacc
     3001 aatggcggcg atgccgacac cctggtgatc tacggcaaga ccgagcccga gctgggcgcg
     3061 cgcggcatga cggccttcat cgtcgagaag ggcttcaagg gcctgagctt cggcaacaag
     3121 ctcgacaagc tgggcatgcg cggctccaac acctggccgg tcttcttcca ggactgcgag
     3181 gtgcccgagg agaacgtgct gggcggcgag ggcaacggcg ccaaggtgct gatgagcggc
     3241 ctggactacg agcgcaccgt gctctccggc ggcccgctgg gcatcatggc cgcctgcatg
     3301 gacgctgtcg tgccctatat ccacgagcgc aagcagttcg gccagagcat cggcgagttc
     3361 cagctgatgc aaggcaaggt ggctgacatg tacaccacct ggcaggccac ccgcgcctac
     3421 gtctatgcgg tgggcaaggc ctgcgaccgc ggcgaccatg cgcgcacgct gcgcaaggat
     3481 gcggccggcg cgattctcta ttcggccgag aaggcaacct ggatggccgg cgaggcgatc
     3541 cagaccctgg gcggcgtggg ctacaccaag gagttcccgg tggagcggct gtggcgtgat
     3601 gcgaagctct acgaaatcgg cgcaggcacg tcggagatcc gccgcatgct gatcggccgt
     3661 gaactcttct ccgaaaccgc ctgatcggcc cagaatccga aacaccggcc tgccgcctcg
     3721 cggagcctga tggagataac aagatgccgg ccctggcttc caaactgaac ccgcgctccg
     3781 aggacttcaa ggccaatgcc gccgtgatgc gcgggctggt cgacgacctc aacgccaagc
     3841 tggcgcagat cgcgcaaggc ggcggcgaag cgccgcgcgc caagcacctg gcgcgcggca
     3901 agctgctgcc gcgcgatcgc gtcgagatgc tgctcgaccc cggcacgccc tttctcgagc
     3961 tcgcgccgct ggccgcgctg gatgtttaca aggagaagga cggcagcgac tcagcgcccg
     4021 gcgccggcgt cgtggccggc atcggccgcg tgaacggcgt cgaatgcatg gtcgtctgca
     4081 acgacgccac cgtgaagggc ggcacctatt acccgctcac ggtcaagaag catctgcgcg
     4141 cgcaggagat cgctgaagcg aaccggctgc cctgcatcta tctggtggac tcgggcggcg
     4201 ccaatttgcc gaaccaggac gaggtcttcc ccgaccgcga tcacttcggc cgcatcttct
     4261 acaaccaggc gaatctctcc gcgcagggca ttccgcagat cgcagtggtg atgggt
//
LOCUS       DASUCY010000042         3904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1414356, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000042 DASUCY010000000
VERSION     DASUCY010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414356"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VFV25_00850"
     CDS             complement(<1..274)
                     /locus_tag="VFV25_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1391892.1"
                     /translation="MHTQTLSFSLRIARTRADLLAACKVRAESYGHHLPNLRAAIGEP
                     DLLDGDEHTHVVLCVDKISGDPVGTARVQTNAGSKLLIEHSTSVPAA"
     gene            413..1546
                     /locus_tag="VFV25_00855"
     CDS             413..1546
                     /locus_tag="VFV25_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HEY1391893.1"
                     /translation="MAWLDRLAAFFIKLCAIAAALAGLGAARADGAALALDKLRVPSG
                     FSIELLARVPNAREMALGDGRILYVGSMHEGKVYALELDEHYRAGTLHVIASGLNLPV
                     GVAYANGSLYVSAVSRILRYDDIAHRLANPPAPVVVRSDLPTETHHGWKFIAFGPDGW
                     LYVPVGAPCNICEPDPDRYALILRMKPDGSQTQVYARGVRNSVGFDWQPGTQELWFTD
                     NGRDMLGDDVPPDKLNHSPRAGLHFGYPYCHGGSIADPEYGRKHACSEFTAPAQALGP
                     HVASLGMRFYTGTQFPAEYRGQIFIAEHGSWNRSHKIGYRVSLVRLAGGKAVSYTPFA
                     TGWLQGESAWGRPADVLVLPDGSLLVSDDYAGAIYRVTYQGPG"
     gene            complement(1550..1705)
                     /locus_tag="VFV25_00860"
     CDS             complement(1550..1705)
                     /locus_tag="VFV25_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1391894.1"
                     /translation="MVTAMLVAACLALAAVLMGAVWSRCGCVDASVPIIMAMLAAGLI
                     AVALYKQ"
     gene            complement(1739..2851)
                     /locus_tag="VFV25_00865"
     CDS             complement(1739..2851)
                     /locus_tag="VFV25_00865"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY1391895.1"
                     /translation="MIALGLVGCSAPQPQTWRVAPTYRVSVTGSASALDYAALARQYE
                     GEQRWGDALNAWSKAASVAPDNAEVLDKLGVALAERGRYDEAVTALRRATELAPTQAR
                     PLNNLGYTLLLAGRTEEARTVLRKAVALDSHYELAGANLQRVDPDGPSARDAAGAPME
                     ATAAAGETESSPASSTKDQRIENLAQSKPIIQLETAAKATALPLQTEPNAPRLALHSE
                     ETGQQPPTRQTAAADPPAASSTALEQEVNGASHPFDGLRIEIANGNGVTGMAARLGSW
                     LRHGGLGMRPRLTNLRPFNTPRTTIYYRSGYAEAAYRLARRMPHEVEVKPQLTSASQV
                     DLRVVLGHDLRQVAACPSDCATLSVTWLETEQSEAR"
     gene            complement(2938..>3904)
                     /locus_tag="VFV25_00870"
     CDS             complement(2938..>3904)
                     /locus_tag="VFV25_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY1391896.1"
                     /translation="SACKESSMNASQLVFLALVFVVVVAAFLGVFALARPSAERRRLS
                     TFVADASSNQPRLGEQWLRWLADLTRPIGKLSMPEEGFEKSSLRLRFIHAGMRNPMAA
                     SIFFGLKTLLTLGLPTLAFVLLALSGSPLRGNNLLLVMLAMATLGYYGPNLVLSHLVM
                     VRQREIFETFPDALDLMTVCVEAGLGTEAAMMRVADDLQFKSPALAEELRLVNLELRA
                     GSDRERALRNLAIRTGVEEVDGFVTMISQAERFGTSIAASLRIHAEMLRTRRRQRAEE
                     AAAKIALKLLFPLIFCIFPSLMVVLMGPAMIQIYRVLLPTMSGQQ"
BASE COUNT          646 a         1291 c         1285 g          682 t
ORIGIN      
        1 tcgccgccgg cacggaagtg ctgtgctcga tcagcagctt gctgccggca ttcgtctgca
       61 cgcgggccgt gccgaccggg tcgccgctga tcttgtcgac gcatagcacc acatgcgtgt
      121 gttcatcgcc gtccagcaga tcgggctcgc ctatggcggc gcgcaggttc ggaagatggt
      181 gtccgtagga ctccgcgcgc accttgcagg cagccagcag atcggcgcgc gtgcgcgcaa
      241 ttctcaagct gaagctgagc gtttgagtgt gcattggact ctcctcgccg catcgaaatg
      301 cgctcccaac ccgcctatgc tagccacatt gtgaaatgca gccaagcaac agcgcactcc
      361 ggccgatccc gctaagcttg cgggctggct gcgaaggaga tggatggtgg gcatggcgtg
      421 gctcgaccgg ctggccgctt tcttcatcaa gctgtgcgct attgctgccg cgctcgcagg
      481 gctgggtgcg gcccgcgccg acggcgctgc gctagcgctg gacaagctgc gggtgccgag
      541 cggcttcagc atcgaactgc ttgcccgcgt gcccaatgcg cgcgagatgg cgctgggcga
      601 tggccgcatc ctctacgtcg gctcgatgca cgaaggcaag gtttatgcgt tggagctgga
      661 tgagcactac cgcgccggca cgctgcatgt catcgccagc ggcctgaacc tgccggtggg
      721 cgtggcctat gcgaacggca gcctttatgt ctccgcggtc agccgcatcc tgcgctacga
      781 cgacatcgcg catcgcctgg ccaatccgcc cgcgccggtg gtagtgagga gcgatctgcc
      841 caccgagacc catcacggct ggaagttcat cgccttcggg ccggacggct ggctctatgt
      901 gccggtgggt gcgccctgca acatttgcga gcctgacccg gatcgctacg ccctgatcct
      961 tcgcatgaaa cccgacggct cgcagacgca ggtctatgcg cgcggcgtgc gcaactcggt
     1021 gggcttcgac tggcagcccg gcactcagga gctctggttc accgataacg gccgcgacat
     1081 gctgggcgac gatgtgccgc ccgacaagct gaaccactcg cccagggccg gcctgcactt
     1141 cggctacccg tattgccacg gcggcagcat cgccgacccc gagtacggca ggaagcacgc
     1201 gtgcagcgaa ttcaccgcgc ccgcccaggc gctgggcccg catgtggcct cgctgggcat
     1261 gcgcttctac accggcacgc agttcccggc ggaataccgt ggccagatct tcatcgcaga
     1321 gcacggctcc tggaaccgca gccacaagat cggctaccgc gtgagcctgg tgcgcttggc
     1381 ggggggcaag gcggtgtcat acacgccctt cgccaccggt tggctgcagg gcgaatcggc
     1441 ctggggccgg ccggccgacg tgctggtgct gcccgacggc tcgctgctgg tgtcggacga
     1501 ttacgcgggg gcgatctatc gggtcaccta ccagggccca ggctaacgct cattgcttgt
     1561 acagcgccac ggcgatcagc ccggccgcca gcatggccat gatgatgggg acgctggcgt
     1621 ccacgcagcc gcagcgtgac caaactgctc ccatcaggac cgctgcaagg gccaggcagg
     1681 ccgcgacgag catcgctgtg accatggcga cacgcatgcc ttggcaaagt gtcgtgtctc
     1741 agcgggcctc gctttgctcc gtctcaagcc aagtcacact cagcgtggca cagtccgacg
     1801 ggcaggccgc gacttgccgc aggtcatggc ccaacaccac gcgcagatcg acctgcgagg
     1861 cactcgtgag ctgcggcttg acctcgacct cgtgaggcat gcgccgggcc agccgatagg
     1921 ccgcctccgc gtagccgctg cggtagtaga tcgtggtcct gggcgtgttg aagggccgca
     1981 ggttggtcaa gcgcggccgc atgcccaggc cgccatgtcg cagccatgaa cccagccgcg
     2041 ccgccatgcc agtcacgccg ttgccgttgg cgatttcgat tcgcagcccg tcgaagggat
     2101 gcgaagcgcc atttacctct tgctccaacg ctgtgctgct ggccgctggc gggtcggcgg
     2161 cagcagtttg ccgcgtgggc ggctgttgcc ccgtttcttc gctgtggagt gccagccgtg
     2221 gcgcgtttgg ctcggtttgc aagggcagcg ccgtcgcctt tgcggcagtt tccagttgga
     2281 tgatcggttt gctctgagcc aggttctcga tgcgctgatc cttggttgaa ctcgccgggc
     2341 tgctttcggt ttctcctgcc gccgccgtcg cttccatcgg tgcaccagcg gcatcgcgtg
     2401 ccgatggacc atccgggtcg acgcgctgca aattggcacc cgcgagttcg tagtgggaat
     2461 ccaatgccac ggccttgcgc aacaccgtgc gcgcttcttc cgttctgccc gcgagcagca
     2521 gcgtgtagcc caggttgttg agcggtctcg cctgcgtcgg agcaagctcc gtcgcgcggc
     2581 gcagagccgt gacggcttcg tcgtaccggc ctcgctcggc cagagccacg ccgagtttgt
     2641 cgagaacctc ggcgttgtcg ggcgccaccg aggcggcctt gctccaagcg ttcagtgcgt
     2701 cgccccagcg ctgttcgccc tcgtattggc gggccagggc cgcatagtcc agggcggacg
     2761 cagaacctgt gaccgagaca cgataggttg gcgccacgcg ccaggtctga ggctgcggcg
     2821 cggagcagcc gacgagtccc agcgcgatca tcaatcccgc agtggggcca tgtctgtgtt
     2881 tgcggcttgt cttcatgagc gcctcctgcg gaagatcacg cgaaccgggc cagatcctca
     2941 ctgctgtccg gacatggtgg gcaacagcac ccgatagatc tgaatcatcg ccgggcccat
     3001 cagaacgacc atcagcgaag ggaagatgca gaagatcagc gggaacagga gcttcagcgc
     3061 gatcttggct gcagcctcct ctgcacgctg gcgccggcgg gtgcgcagca tctcggcgtg
     3121 gatgcgcaga gacgcggcga tgctggtgcc gaaacgctcg gcctggctga tcatggtcac
     3181 gaagccgtcc acctcttcca cgccagtgcg tatcgccagg ttgcgcagcg cccgctcgcg
     3241 gtcggagccg gcgcgcagct cgaggttgac caggcgcagt tcttcggcca gggcagggct
     3301 cttgaactgc aggtcgtcgg caacgcgcat catggcggcc tcggtaccca gccccgcttc
     3361 cacgcacacc gtcatcaggt ccagcgcatc cggaaaggtt tcgaatatct cgcgctggcg
     3421 caccatcacg aggtgcgaca gcacgagatt gggcccgtaa taccccaggg ttgccatcgc
     3481 cagcatcacg agcagcaagt tgttgcccct caacggcgag cccgacagcg ccagcaggac
     3541 gaaggcgagc gtcggcaagc ctagcgtcag gagcgtcttg aggccgaaga agatcgaagc
     3601 ggccatgggg ttgcgcatcc cggcatggat gaagcgcagc ctgaggctcg atttctcgaa
     3661 gccttcttcg ggcatcgaca gcttgccgat cgggcgcgtc agatccgcca gccagcgcaa
     3721 ccactgttcg cctagccgag gctggttgct gctcgcatcg gccacgaagg tggaaaggcg
     3781 ccgccgttcg gcgctgggac gcgccagggc gaatacgccc aggaaggcgg cgacgaccac
     3841 cacgaaaacg agtgcaagaa agacgagctg gctcgcattc atcgaagact ccttacacgc
     3901 ggat
//
LOCUS       DASUCY010000043         2508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1205591, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000043 DASUCY010000000
VERSION     DASUCY010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205591"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2373
                     /locus_tag="VFV25_00875"
     CDS             <1..2373
                     /locus_tag="VFV25_00875"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEY1391897.1"
                     /translation="GAAAYLARQYKTIAMVGVVLAVLIAIFIGLPTAAAFVVGAILSG
                     ACGFIGMNVSVRANVRTAQAATRGIGEALNVAFKGGAITGMLVVGMGLLGVGIVFWVL
                     TRHGAGANLATTIKPLLGLAFGASLISIFARLGGGIFTKGADVGADLVGKVEAGIPED
                     DPRNPAVIADNVGDNVGDCAGMAADLFETYAVTIIATMALGALLITSAPLQAVAYPLA
                     LGGFSIIASIIGCSFVKAKPEMKNVMPALYKGLIITGLISLVGFYFITNSLMPDDALG
                     APGSQIKLFGSAVVGLVLTALLVWITEYYTGTDYAPVRHVAKASTTGHGTNIIAGLGV
                     SMKSTAWPVVCVCLAILVSYWLGGLYGIAIAATSMLSLAGIVVALDAYGPITDNAGGI
                     AEMAGLPQSVRDITDPLDAVGNTTKAVTKGYAIGSAGLAALVLFADYTHALSASGHNI
                     SFDLSNHKVIIGLFIGGMIPYLFGAMAMEAVGRAAGAVVEEVRRQFRDIKGIMDGSGK
                     PEYGRAVDMLTTAAIKEMIIPSLLPVVVPIAVGLILGPTALGGLLMGTIVTGLFVAIS
                     MCTGGGAWDNAKKYIEEGNYGGKGSETHKAAVTGDTVGDPYKDTAGPAVNPLIKIINI
                     VALLIVPLRPVAVATGGGAAGEHASAAVATVATTPAAATTAANATTANGDEGSTVKTG
                     TDANGKLQGEAKIYFGSGKAALPAGANEDLAKLAEAVRSGSGKLVISGYHDASGDPAK
                     NAELAKERAQNVATALEAAGVAKDRIELKKPEQVDGGVSAEARRVEVKLE"
BASE COUNT          414 a          841 c          852 g          401 t
ORIGIN      
        1 ggcgcggcgg cctatctggc gcgccagtac aagaccatcg ccatggtcgg cgtggtgctg
       61 gccgtgctga tcgcgatctt catcggcctg ccgacggcgg cggccttcgt ggtcggcgcg
      121 atcctgtcgg gcgcctgcgg cttcatcggc atgaacgtct cggtgcgcgc caacgtgcgc
      181 accgcgcagg ccgcgacgcg cggcatcggc gaggcgctca acgtggcctt caagggcggg
      241 gcgatcaccg gcatgctggt ggtcggcatg ggcctcttgg gcgtgggcat cgtgttctgg
      301 gtgctgacac gccacggcgc gggcgcaaac ctggccacga cgatcaagcc gctgctgggc
      361 ctggccttcg gcgcctcgct gatctccatc ttcgcgcgcc tgggtggcgg catcttcacc
      421 aagggcgccg acgtgggcgc cgatctggtg ggcaaggttg aggccggcat cccagaggac
      481 gacccgcgca acccggccgt gatcgctgac aacgtgggcg acaacgtggg cgattgcgcc
      541 ggcatggcgg ccgacttgtt cgagacctat gccgtcacca tcatcgcgac gatggcgctg
      601 ggcgcgctgc tgatcacctc ggcgccgctg caggcggtgg cctatccgct cgcgctgggc
      661 ggcttctcca tcatcgcctc catcatcggc tgcagcttcg tcaaggccaa gccggagatg
      721 aagaacgtga tgccggcgct ctacaagggc ctgatcatca cgggcctcat ctcgctggtg
      781 ggcttctact tcatcaccaa ttcgctaatg cccgatgacg cactgggtgc gcccggcagc
      841 cagatcaagc tgttcggctc ggcagtcgtg ggcctggtgt tgacggcgct gctggtgtgg
      901 atcacggagt actacaccgg caccgactac gcgccggtca ggcatgtggc caaggcctcg
      961 accaccggcc acggcaccaa catcatcgcc ggcctgggcg tgagcatgaa gtccaccgcc
     1021 tggcccgtgg tctgcgtgtg cctggccatc ctggtgtcct actggctggg cgggctctac
     1081 ggcatcgcca ttgccgcgac ctcgatgctg agcctggccg gcatcgtggt ggcgctggac
     1141 gcctacggcc ctatcaccga caacgccggc ggtatcgccg agatggccgg cttgccgcaa
     1201 tcggtgcgcg acatcaccga cccgctggac gcggtgggca acaccaccaa ggctgtcacc
     1261 aagggctacg cgatcggctc ggccggcctc gcggcgctgg tgctgttcgc cgactacaca
     1321 catgcgctga gcgcctcggg ccacaacatc agcttcgatt tgtccaacca caaggtgatc
     1381 atcggcctct tcatcggcgg catgatcccc tatctcttcg gcgcaatggc gatggaggcc
     1441 gtgggccgcg ccgcgggtgc agtggtcgag gaagtgcgcc gccagttccg cgacatcaag
     1501 gggatcatgg acggctcggg caagccggag tacggccgcg cggtcgacat gctgacgacc
     1561 gccgcgatca aggaaatgat catccccagc ctgctgcccg tggtggtgcc cattgccgtg
     1621 ggcctcatcc tcggcccgac agcactcggc ggcctgttga tgggcaccat cgtgaccggc
     1681 ctcttcgtcg cgatctcgat gtgcaccggc ggcggcgcct gggacaacgc caagaagtac
     1741 atcgaggagg gcaattacgg cggcaagggc agcgagacgc acaaggccgc cgtcacaggc
     1801 gacaccgtgg gcgatcccta caaggacacg gccggcccgg cggtgaaccc gctgatcaag
     1861 atcatcaaca tcgtggccct gctgatcgtg ccgctgcggc cggtggcggt ggcgaccggc
     1921 ggcggcgcgg ccggcgagca tgcgtccgcc gccgtggcga cagtggcaac cactcccgcc
     1981 gccgcgacca cggccgccaa tgcaacgacg gccaatggcg acgaaggcag caccgtaaag
     2041 accggcaccg acgccaacgg caagctgcaa ggcgaggcga agatctactt cggcagcggc
     2101 aaggccgcct tgcctgcggg cgccaatgag gatttggcca agctcgccga ggccgtgcgc
     2161 tcaggcagcg gcaagctggt catcagcggc tatcacgacg ccagcggcga cccggccaag
     2221 aacgccgaac tggccaagga gcgcgcgcag aacgtcgcca cggcgctcga agccgcgggc
     2281 gtggccaagg accgcatcga gctcaagaag cccgagcagg tcgacggcgg cgtctcggcc
     2341 gaggccaggc gcgtggaggt gaagctggaa tagccagcct gcgcttacga gaaaggcccg
     2401 catcgcgggc ctttttcttt gccgatgggt gttactacgc tgttgaccca aacgctcgtc
     2461 ccgaggcttg tcgttgctga gtctccggag ccctagagaa ctttctct
//
LOCUS       DASUCY010000044         4573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1077085, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000044 DASUCY010000000
VERSION     DASUCY010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4573
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077085"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1563)
                     /locus_tag="VFV25_00880"
     CDS             complement(<1..1563)
                     /locus_tag="VFV25_00880"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY1391898.1"
                     /translation="MPFMKIRRLALALSLGGLVLVVAGLVTARYFAPPELRWRLQILA
                     AKLHGDLGYIPARNLIAWLAPGSPVYLAPLADNPNPHLGIKNEYKKLEDARQGKLLYL
                     RRCSQCHGDNAHGNSGPDLLASVSGKSDWSFFSTAKWGRQGTSMQAQALTDQQIWQVH
                     AYLRSEALASGRATAGDKSASARPLVDIAAQQIIDTDANPGQWLTYAGNYAGHRHSLL
                     PQLTKSNVGNLRLAWVAQLRQVDRDLQVSPIMAGGVMYVTESREGVVALDAKTGAVLW
                     TFQRPVPDGVRGCCGMPNRGVAVLGQTVFVASADAYLIALDANTGQQRWITKVADYQA
                     GYTITGAPLAVGGRVVTGIAGGEFGIRGFLAAFDAATGRPLWTFNTVPGPGEAAHDTW
                     TGDSWKTGGAPTWTVGAYDPEQDLIIWGTGNPSPEFQAKLRPGDNLYSNSVIAVEAKT
                     GRLRWHYQFTPADEHDWDSCQQPILADIVWQGRKRAVVLWANRNAFFYALDRKSGEFL
                     FAKPFVKQSWNEG"
     gene            1908..2885
                     /locus_tag="VFV25_00885"
     CDS             1908..2885
                     /locus_tag="VFV25_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1391899.1"
                     /translation="MGTASQAWYRRATPVLYALLICCSLLAALVYRLRVDGIFACPAS
                     GYGSAAYLSECSAGQYGDYDHGAFWFELEPQARRAAAEAEVLFVGSSRLQFALSGGNA
                     AAWFSAAGVRYYLLGFSHSETVAFFGPLLAKLQPHAKVVVINVDRIFDERISPPTEQI
                     LQGHDILQKYEEKRSWQALHKGLCTALPAVCGDAVAVYRERETGVWHRTGAGRFKALE
                     VSEGPPSNDARWAGYARLGEQFLARLPVDRSCIILTIVPTVETKRDEARAIAQKLGLD
                     LLEPRVDGLRTFDGSHLDGGSAERWSEAFLREAGPRIRRCADPPRDSMG"
     gene            2931..>4573
                     /locus_tag="VFV25_00890"
     CDS             2931..>4573
                     /locus_tag="VFV25_00890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIC family phosphatase"
                     /protein_id="HEY1391900.1"
                     /translation="MDPTSGHGDFSSQLQAVLAQADARARLDGLTRLAQACRTFVEAI
                     QLDRAIAQQGNEESLRGLACLRLAVLSTHTIDHLLPAIRVAGFGRGFRLEAYAGAFGQ
                     YRQELLDPSSALHRFRPNLVLFSLAAGQAVGGVPLAAAAAEVDAMLARTIDELRELWR
                     HARLRLDAAVIQQSYLDVSQPLFGSYDRLVPAAPSRMILRLNELLAQAVAQEGVLLLD
                     VAHLAAQDGIDAWFDVARWLQGKMEISPQAAPRYAELLMRVIGAMRGKSRKCLVLDLD
                     NTLWGGVLGEVGMTGLALGEGSAQGEAHLALQRYAKSLKERGVVLAVCSKNDQEAAEA
                     VFRDHPEMVLRRGDIAAFVANWQDKVENLKDIARSLNIGLDSVVFVDDNPVERARVRQ
                     GLPVVAVPELPDDPAFYVRCLAAGGYFEAVSFTQEDLRRGEQYAMNAQREALRGMTSS
                     LDNFLRGLEMSVVYGPIAEVERERAAQLINKTNQFNLTARRGTVDEFARLCEAPVNLA
                     LQFRLIDRFGDNGLVSVMILAPDADADADDARALRVDTWV"
BASE COUNT          721 a         1508 c         1553 g          791 t
ORIGIN      
        1 gccttcgttc cagctctgtt tcacgaaagg cttggcaaac aggaactccc cgctcttgcg
       61 gtccagcgca tagaagaagg cattcctgtt ggcccacagc accaccgcgc gtttgcgccc
      121 ctgccagacg atgtccgcca ggatgggctg ctggcacgaa tcccagtcat gctcgtccgc
      181 cggcgtgaac tgatagtgcc acctcaggcg gccggtcttg gcctcgaccg cgatcacgct
      241 gttgctgtat aggttgtcgc cgggccgcag cttcgcctgg aattcgggcg aagggttgcc
      301 ggtgccccag atgatcaggt cctgctcggg atcgtaggcg cctacggtcc aggtgggagc
      361 gccgccggtc ttccagctgt cgcccgtcca tgtgtcatgc gccgcttcgc ccggcccggg
      421 gacggtgttg aaggtccata gcggacgtcc cgtcgcagcg tcgaacgccg ccaggaagcc
      481 ccggatcccg aactcgcccc cggcgatccc ggtgacgaca cgccccccga cagccagcgg
      541 tgcgcccgtg attgtgtagc ccgcctgata gtcggcgacc ttcgtgatcc agcgctgctg
      601 gccggtgttg gcgtcgagcg cgatcaggta ggcatcggcg ctggccacga agacggtctg
      661 gccgagcacg gccacgccgc ggttgggcat tccgcagcag ccgcgaactc catcgggaac
      721 gggtctttga aacgtccaga gcaccgcgcc ggtcttggca tccagcgcca ccaccccctc
      781 gcgcgactcc gtcacgtaca tcaccccgcc cgccatgatc ggggatacct gcaagtccct
      841 gtcgacctgg cgcagttgcg cgacccaggc caggcgcagg ttgccgacat tgctcttcgt
      901 caactgcggt agcaggctgt gcctgtggcc agcgtagttg cctgcgtagg tcagccattg
      961 gccggggttc gcgtcggtgt cgatgatctg ctgcgcggcg atgtcgacca ggggacgggc
     1021 gctggctgat ttgtcacccg cggtcgcccg acccgaggcg agcgcctcac tgcgcagata
     1081 ggcatggacc tgccagatct gctggtccgt gagggcttgc gcctgcatgg aggttccctg
     1141 gcgtccccac ttggccgtgg agaagaacga ccagtcggac ttgccggaca ccgaggcgag
     1201 gaggtccggg ccggaattcc catgcgcgtt gtcgccgtgg cattggctgc agcgcctgag
     1261 gtagagcagc ttgccttggc gcgcgtcctc gagcttcttg tactcgttct tgatgccgag
     1321 gtgaggattg ggattgtcgg ccagcggtgc cagatagacc gggctgccgg gcgccagcca
     1381 ggcgatcaga ttgcgagccg ggatgtagcc caagtcgccg tgcagcttgg cagccaggat
     1441 ctgcaggcgc caccgcagct ccggcggggc gaaatagcgc gccgtcacca ggccggcgac
     1501 gaccaggacc agccctccga gagagagcgc cagcgcaagt cgtcgaatct tcatgaacgg
     1561 catggcgtaa tcggcatgga caaagcgttc atctggtccc gaatgaatgc cgccggagtc
     1621 ggcgacgacg cggcaattgg agcattgcgc aggcttgtcg cgcgtcgatt cgggacacgc
     1681 gattcgaggg ccgcgtcccc cctcgaatgt gggggtggtc gccgaagagg aaatctgaaa
     1741 gattggatgc gccttgcaat caatgctgtc gccggccggc gggaaagaac cgctgcggat
     1801 cagcgatgac gaaaggcact ggtggccact ggcatgggcg ccagtatcgg ctccgcgcag
     1861 ctcttttgga ctgagccttg cagatgagca cttcggcttc cggtttcttg gggacggctt
     1921 cacaggcttg gtaccgtcgc gcgaccccgg tgctgtacgc gctgctgatc tgctgcagtc
     1981 tgctcgcggc gcttgtctat cggctgcgcg tcgacggcat cttcgcctgt cctgcgagcg
     2041 gctatggctc cgcggcctat ctgtccgaat gctcggccgg ccagtacggg gactacgacc
     2101 atggcgcctt ctggttcgag ctggagccgc aggcgcgccg cgcggctgcc gaggccgaag
     2161 tactcttcgt cggcagcagc cggctgcaat ttgcgctgtc cgggggcaat gccgccgctt
     2221 ggttctcggc agccggcgtg cgctactacc tgctcggctt cagccacagc gaaaccgttg
     2281 ccttcttcgg cccgctgctt gcgaagctgc agccgcatgc caaggtcgtc gtgatcaacg
     2341 tcgaccgcat cttcgacgag cgcatttcac cgcccaccga gcagatcctg caagggcacg
     2401 acatcctgca gaagtacgag gaaaagcgca gctggcaggc attgcacaag gggctgtgca
     2461 cggctttgcc ggccgtatgc ggagatgcgg tggccgtcta ccgcgagcgc gaaacggggg
     2521 tgtggcacag gacgggggca gggcgcttca aggccttgga ggtgtctgaa ggtccaccga
     2581 gcaacgacgc gcgctgggct ggctatgcga ggctcggcga gcagttcctc gcgcggctgc
     2641 cggtggatcg aagctgcatc atcctcacca tcgtcccgac ggtggaaacc aagcgggacg
     2701 aagcccgggc gattgcgcag aagctggggc tggatctgct cgagcccagg gtcgacggac
     2761 tgcgcacctt cgacggctcc catctcgatg gcggcagcgc ggagcgctgg tccgaggcct
     2821 tcctgcggga ggccgggccg cgcatccgca ggtgcgccga cccgccacgg gactccatgg
     2881 gttagcggcc gtacgagccg aaaccacatt gcctagctct cgctctcatc atggatccga
     2941 catccggcca cggcgacttc agctcgcaac tgcaggccgt attggcccag gcggatgcac
     3001 gcgcccgcct cgacggcctg acccgtcttg cgcaggcttg taggaccttc gtcgaggcca
     3061 tccagctcga tcgggcgatc gcgcagcaag gcaatgagga atcgcttcgc gggctggcct
     3121 gcctgcgcct ggcagtgctg tccacgcaca cgatcgatca cctgctgccg gcgatccgtg
     3181 tcgccggctt tggccgcgga tttcgcctgg aggcctacgc cggcgccttc ggccagtacc
     3241 gccaggaact gctcgatccc tcctcggccc tgcatcgctt ccggcccaac ctggtgctgt
     3301 tctcgctggc ggccgggcag gccgtcggcg gcgtgccgct cgccgcggca gcggcagaag
     3361 tggacgcgat gctcgcgcgc actatcgatg aactgcgcga actctggcgg cacgcgcggc
     3421 ttcgcctcga tgcagccgtc atccagcagt cctacctcga cgtgtcgcag ccgctgttcg
     3481 gcagctacga ccggctggtc ccggccgcgc cgtcgcgcat gatcctgcgc ttgaacgagc
     3541 tgctcgccca ggccgtggcg caggagggcg tgctgctgct ggatgtcgca catctggccg
     3601 cgcaggacgg catcgacgcc tggttcgatg tcgcgcgctg gctccagggc aagatggaga
     3661 tctcgccgca agcggcgccg cgctacgccg aactgctgat gcgcgtgatc ggcgccatgc
     3721 gtggcaaatc ccgcaagtgc ctggtgctgg atctggacaa cacgctctgg ggtggcgtgc
     3781 tgggcgaggt gggcatgacc ggcctcgcgc tgggcgaagg cagcgcgcag ggcgaggcgc
     3841 acctcgcctt gcagcgctat gccaagtcgc tgaaggagcg cggcgtggtg ctcgccgtct
     3901 gttccaagaa cgatcaggaa gcggccgagg cggtgtttcg cgatcatccc gagatggtgc
     3961 tgcggcgcgg cgacatcgcc gccttcgtcg ccaactggca ggacaaggtc gagaacctca
     4021 aggacatcgc gcgcagcctc aacatcggcc tggacagcgt ggtcttcgtc gacgacaacc
     4081 cggtggaacg cgcgcgtgtg cgccagggcc tgccggtcgt ggccgtgccc gagctgccgg
     4141 acgatccggc cttctatgtc cgctgcctcg ccgcaggcgg gtatttcgag gccgtgagct
     4201 tcacccagga ggatctgcgt cgcggcgagc aatacgcgat gaacgcgcaa cgcgaggcct
     4261 tgcgcggcat gacgagcagc cttgacaact tccttcgcgg gctcgagatg tcggtcgtct
     4321 acgggccgat cgcggaggtg gagagggagc gcgccgctca gctgatcaac aagacgaacc
     4381 agttcaacct cacggcgcgg cgcggcaccg tagacgagtt cgcccgcctg tgcgaagcgc
     4441 cggtgaacct cgcgctccag ttccgcctca tcgatcgctt cggcgacaac ggcctggtga
     4501 gcgtgatgat cctcgccccc gacgccgacg ccgacgccga cgatgcgcgg gcgctcaggg
     4561 tcgacacctg ggt
//
LOCUS       DASUCY010000045         2310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1350213, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000045 DASUCY010000000
VERSION     DASUCY010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1350213"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VFV25_00895"
     CDS             <1..533
                     /locus_tag="VFV25_00895"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEY1391901.1"
                     /translation="YYRLNGRSVADMEWQRERFRTHIRAARAVGKPLVIHTRSASEDT
                     IDILRTEGEDGSAGSAGGVFHCFTETRGVARAALDLGFYISFSGILTFKNAQELQEVA
                     KEVPLERCLIETDSPYLAPVPHRGKTNRPSYVPHVARRLAELKGCSLEEVAAATSRNF
                     ERLFGLPALQNEPLSA"
     gene            complement(618..1334)
                     /locus_tag="VFV25_00900"
     CDS             complement(618..1334)
                     /locus_tag="VFV25_00900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HEY1391902.1"
                     /translation="MPSSDVSTPPRCLIARIAFALLPLCLAGSASAQMAISVTAESDY
                     RFRGVSLSDEKPSLHLSLGYDGASGWYGGATAATVELEPDRRIAQLLGYAGYAARAAS
                     GLGWELGATQAHFDGSSRYDYGEFYGGLNGQGWNARLYYSPSYFGRGVRTAYAELNGG
                     WPQEGLLRLFAHVGALLPLGSSQGSPRNRYDLRLGVSAALVRSGEVQLAWVAASRGGP
                     YPAIYEQKRGALVLSASLFF"
     gene            complement(1321..>2310)
                     /locus_tag="VFV25_00905"
     CDS             complement(1321..>2310)
                     /locus_tag="VFV25_00905"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEY1391903.1"
                     /translation="LGVDCYVMAVPPDRAPEHVAQVLSQDARVAWAQPMNIYRAQQQA
                     AAAAPTHNDPLYPAQPAAAAWHLAALHELATGREVRVAVIDSGIEQNHPDLDGQVALA
                     RNFVPARPDAPEQHGTAVAGIIAARADNGIGIAGVAPRARLLALRACWQESRSSTLCS
                     SLSLAMALDFAISHDAQVINLSLGGAPDRLLGKLLDAALVRGITVVAAADRSLPGGGF
                     PAGHPGVVAVSDEAPNPPAGALLAPGRDVPTTTLGAHWNLVSGSSYAAAHVSGLFALV
                     RELHRSPLPAASTTLSVLPSGGIDTCATLLRFVSPRACACANMRATPPPIAQQ"
BASE COUNT          399 a          780 c          781 g          350 t
ORIGIN      
        1 actactaccg gctcaacggc cgcagcgtgg ccgacatgga atggcagcgc gagcgctttc
       61 gcacgcatat ccgggccgcc cgagcggtgg gcaagccgct cgtgatccac acgcgcagcg
      121 ccagcgagga cacgattgac atcctcagga ccgagggcga ggacggcagc gcaggctcgg
      181 caggcggtgt cttccattgc ttcaccgaga cgcgcggggt cgcgcgggcc gcgctggacc
      241 tcggcttcta catctccttc tcgggcatcc tgaccttcaa gaacgcccag gagctgcagg
      301 aggtggcgaa ggaagtaccg ctggagcgct gcctgatcga aaccgacagc ccctatctgg
      361 cgccggtgcc ccatcgcggc aagaccaaca ggccctccta tgtgccccac gtggcccgcc
      421 gactggctga gttgaaaggc tgcagcctgg aagaggtcgc tgcggcgacc agccgcaact
      481 tcgagcggct attcggcctg ccggccttgc agaatgagcc gttgagtgct tgagaaagtt
      541 gatttattgc atgagatata ttttctatct cattgtcaat aaacaaattt agtgccgact
      601 gtgcgcagcc gttcctttta gaagaagagc gatgcgctga gcaccagggc cccgcgcttt
      661 tgctcataga ttgccggata ggggccgccg cggctcgccg ccacccaggc tagctgcact
      721 tcgccagagc gaacgagagc cgcgctcacg cccaggcgca ggtcatagcg gttgcgtggc
      781 gagccttgac tggacccgag cggcagcaag gcacccacgt gagcgaagag ccgcaacagc
      841 ccttcttgcg gccagccgcc gttgagctcg gcataggcgg ttcgcacgcc gcggccgaaa
      901 tagctgggcg agtagtacag cctcgcgttc cagccctgtc cgttcaggcc gccgtagaac
      961 tcgccatagt cgtagcgcga gctgccgtcg aaatgcgcct gcgtcgcccc cagctcccag
     1021 cccaaccccg aggcagcgcg cgccgcatag cccgcatagc ccagcagttg ggctatgcgg
     1081 cgatccggtt cgagttcgac tgtcgcggct gtcgccccgc cataccagcc gctggcaccg
     1141 tcatagccca ggctcagatg caggctgggc ttttcgtcgc tgagcgacac gccgcgaaag
     1201 cggtagtcgc tctccgccgt cacgctgatc gccatctgcg ccgatgcgct gccggccaga
     1261 cacaggggca gcaaggcgaa ggcgatgcgg gcaatcaggc agcgcggggg cgtcgacaca
     1321 tcactgctgg gcaatgggtg gtggtgtggc gcgcatattg gcacaggcgc aggcccgcgg
     1381 gctgacaaag cgcagcagcg tggcgcaggt atcgatgccg ccgctgggca gcacgctcag
     1441 ggtcgtcgac gcggcgggga ggggggagcg gtgcaactcg cgcaccaggg cgaacaggcc
     1501 gctcacatgg gcagcggcat aagacgagcc ggacaccaga ttccaatggg ccccgagcgt
     1561 cgtggtcggc acgtcacgcc ctggcgccag cagagccccg gctggagggt tgggcgcctc
     1621 gtccgatacc gccaccacgc cgggatggcc ggctgggaag ccgccccccg gcaggctgcg
     1681 atccgcagcg gcgaccaccg tgatgccgcg caccagcgcc gcgtcgagca gcttgccgag
     1741 cagccggtct ggcgcgccac ccaggctcag attgatcacc tgggcgtcat gcgagatggc
     1801 gaagtccagc gccatcgcga ggctcaagct gctgcacaag gtgctcgacc ggctctcctg
     1861 ccagcaggcg cgcagcgcca gcagccgggc cctgggcgcc acgcccgcga tgccaatgcc
     1921 gttgtcggcg cgggcagcga tgatgccggc cacggccgtg ccatgctgct cgggcgcatc
     1981 cggccgcgcg ggaacgaagt tgcgggcgag cgccacctgg ccatccaggt cgggatggtt
     2041 ctgctcgata ccgctgtcga tcaccgcgac ccggacttcg cgcccggtcg ccaattcatg
     2101 cagcgcagcc agatgccagg cggccgccgc cggctgcgcg gggtagagcg gatcgttgtg
     2161 cgtgggcgcc gctgccgccg cttgctgctg ggcccggtag atgttcatcg gctgtgccca
     2221 ggccactctt gcgtcctggg acaagacttg cgcaacatgt tcgggcgcgc gatcgggagg
     2281 cacggccatc acatagcagt ccacacccag
//
LOCUS       DASUCY010000046         2998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1494872, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000046 DASUCY010000000
VERSION     DASUCY010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494872"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1293)
                     /locus_tag="VFV25_00910"
     CDS             complement(<1..1293)
                     /locus_tag="VFV25_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEY1391904.1"
                     /translation="MSFPPLSRCAGWLLGLVSLLAGAVHAQTPSADDTQSPAAAPAPV
                     SASAQRLYEAARQQLLQVRTLLKGQDSQAVVGSGFIVSEQGHILTNYHVVSQAALQPE
                     RYRLVYSTADRKEGALQLLAFDAIHDLALVKPVQPEALAGRGALRFRPRQLAVAQGER
                     IYSLGNPLDVGFAVLEGTYNGLVARSFYPTIFFAGSLNAGVSGGPTLDGEGRVTGVNV
                     AARRDGGQVSFLVPASYAEDLLRKGRDAKPISAPVYPELARQLLAHQQTLTERFLAQP
                     WRHAGHARYLIPLPQEDFMRCWGRSTPADAKGLDYERSDCMMDTRIFVSGWLTTGSLQ
                     VRHEAYDGRKLGPLRFASQYSDSFRNESFGERADANKTAAQCSERFVDREGLPLRAVL
                     CMRAYKKLAGLYDLSVLVATLDQSTAGVQGRFDARGVSF"
     gene            1437..1994
                     /locus_tag="VFV25_00915"
     CDS             1437..1994
                     /locus_tag="VFV25_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEY1391905.1"
                     /translation="MDVLQLDVSGRPQAWISAKEAAVIYACDGVAWTLGDPCLVMRGG
                     TQRITGLQSRIEVHPIIAVRGAIPSRAWRQVPALSNPKLFGRDRLLCAYCGDQFHVDD
                     LTREHIRPISRGGADTWMNCITACRGCNGHKGNRLPEEAHMSLLYLPYVPSLHEDMIL
                     RGRRILADQMEFLLASVPRHSRLHA"
     gene            2081..2557
                     /locus_tag="VFV25_00920"
     CDS             2081..2557
                     /locus_tag="VFV25_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEY1391906.1"
                     /translation="MKKLLALAAAAALTACATSSPDVVQRYDANRMSQVQDAVVINVR
                     PVTVDGSQSGGGAAAGGIIGGVAGSSVGGNRESIAVGVLGAVAGAVVGNAVERGVTRE
                     NANEILVQLTSGERRSVVQAQGNEQFAPGDAVILVTTGGHTRVMKAPPRAAPRSPS"
     gene            2720..>2998
                     /locus_tag="VFV25_00925"
     CDS             2720..>2998
                     /locus_tag="VFV25_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003261357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome B"
                     /protein_id="HEY1391907.1"
                     /translation="MSMAIALIVIVVASVLFHLISPWWLTALASNWQRMDDTLTITLV
                     ITGIFFVVINLFVVYTLLRYRHRQGHRAAYEPENSRLERWLIGVTTVGI"
BASE COUNT          482 a         1042 c         1027 g          447 t
ORIGIN      
        1 gaagctgacg ccgcgcgcat cgaagcggcc ctgcacgccg gccgtggatt gatcgagcgt
       61 ggccaccagc acgctcaagt cgtagagccc ggccagcttc ttgtaggcgc gcatgcacag
      121 cacggcgcgc agcggcaggc cctcgcggtc gacgaagcgc tcgctgcatt gcgcggcggt
      181 cttgttggca tcggcgcgct cgccgaagct ctcgttgcgg aagctgtcgg aatattggct
      241 ggcgaagcgc agcggcccca gcttgcggcc gtcataggcc tcatggcgca cctgcagcga
      301 accggtggtg agccagccgc tgacgaagat gcgcgtgtcc atcatgcagt cggagcgctc
      361 gtagtccagg cccttggcat cggccggcgt gctgcggccc cagcagcgca tgaagtcctc
      421 ctgcggcagc gggatcaggt agcgcgcatg gccggcatgg cgccagggct gggccaggaa
      481 gcgctcggtc agcgtctgct ggtgggcgag cagctggcgc gccagctccg gatagaccgg
      541 cgcgctgatc ggcttggcat cgcggccctt gcgcagcaga tcctcggcat agctcgcggg
      601 caccaggaag ctgacctgcc cgccgtcgcg ccgcgccgcc acgttgacgc ccgtcacgcg
      661 gccctcgccg tccagcgtgg gcccgccgct cacgccggca ttgagcgagc cggcgaagaa
      721 gatggtgggg tagaagctgc gcgccaccag gccgttgtag gtgccttcca gcaccgcgaa
      781 gcccacgtcc agcgggttgc ccagcgaata gatgcgctcg ccctgggcca ccgcgagctg
      841 gcgcgggcga aagcgcaacg cgccgcgccc ggccagagcc tcgggctgca ccggcttgac
      901 caaagccagg tcgtggatcg catcgaaggc cagcagctgc agcgcgccct ccttgcggtc
      961 ggcggtgctg tagaccaggc gatagcgctc gggctgcagc gcggcctggc tcacgacgtg
     1021 gtagttggtg aggatgtggc cctgctcgct gacgatgaag cccgagccga ccacggcctg
     1081 actgtcctgg cccttgagca gcgtacgcac ctgcagcaac tgctggcgcg ccgcctcgta
     1141 aagccgctgg gcggaggccg aaacgggggc cggtgcggcc gccgggctct gcgtgtcatc
     1201 ggcagatggg gtctgcgcat ggacggcgcc ggcgagcagc gagaccaggc cgagcagcca
     1261 gccggcgcac cgtgaaagag gcggaaagct catcggacca ttctagggag cggccgcgcc
     1321 tcgacgatgc aatggtgtcc ggcgtaggca atcccccacg ccgacaagcg ccggcggccc
     1381 ggaaagttga gcattgacac ggtgttgctt catactcaca gcttcaggag cgggccatgg
     1441 acgtactgca actcgacgtg tccggccgcc cgcaggcctg gatctcggcc aaggaagctg
     1501 ccgtcatcta cgcctgcgac ggcgtagcct ggactttggg cgatccctgc ctcgtgatgc
     1561 gcggcggcac gcagcgcatc accgggctgc aatcgcgcat cgaggtgcat cccatcattg
     1621 cggtacgcgg cgcgattccc agccgcgcct ggcgccaggt gccggcgctg tccaatccca
     1681 agctcttcgg ccgcgaccgc ctgctgtgtg cctattgcgg cgaccagttc catgtcgacg
     1741 acctgacgcg cgagcacatc cggccgatct cgcgcggcgg cgccgacacc tggatgaact
     1801 gcatcactgc ctgccgcggc tgcaacggcc acaagggcaa ccgcctgccc gaagaggccc
     1861 atatgtcgct gctctacctg ccctatgtgc ccagcctgca cgaggacatg atcctgcgcg
     1921 gccggcgcat cctggccgac cagatggagt tcctgctggc cagcgtgccg cggcacagca
     1981 ggctgcacgc gtgagcgtgt aggaacccca gccgctggga gccgatccaa agagcggcat
     2041 actgccggat tcacagattg atacacgcga ggatgacgcc atgaagaaac tgcttgccct
     2101 tgcagccgcc gcggccctga ccgcctgcgc caccagcagc cccgacgtcg tgcagcgcta
     2161 tgacgccaac cgcatgtcgc aggtgcagga cgccgtggtg atcaatgtgc ggcccgtgac
     2221 agtcgacggc tcgcaaagcg gcggcggcgc ggccgccggc ggcatcatcg gcggcgtggc
     2281 cggctcctcg gtgggcggca accgcgagtc gatcgccgtc ggcgtgctcg gcgcggtggc
     2341 cggtgccgtc gtgggcaatg ccgtcgaacg cggcgtcacg cgcgagaacg ccaacgagat
     2401 cctggtgcaa ctgaccagcg gcgagcgccg ctccgtcgtg caggcccagg gcaacgagca
     2461 attcgcgccc ggcgacgcgg tcatcctggt caccaccggc ggccacacgc gcgtgatgaa
     2521 ggcgccgccg cgcgccgcgc cgcgcagccc gagctgagca cgcctcggct ccggtcatac
     2581 acagcagcca ccggccgcag cgaagagctg cgtccgggat ggatcgcttg tagcggcatc
     2641 gccataggag taggctaggg ccgctcccgc aacgcgggcc ggcactggcc ttcgcgggac
     2701 ttgcgagaag aggctgccca tgtccatggc catcgccctg atcgtgatcg tcgtcgcctc
     2761 ggtgctgttc cacctgatca gcccgtggtg gttgaccgcg cttgcctcca attggcagcg
     2821 catggacgac acgctgacga tcacgctcgt gatcaccggc atcttcttcg tcgtcatcaa
     2881 tctcttcgtt gtctacacgc tgctgcgcta tcgccaccgc cagggccatc gcgcggccta
     2941 cgagcccgag aacagccggc tggagcgctg gctcatcggc gtcaccacgg tgggcatc
//
LOCUS       DASUCY010000047         3916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_900416, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000047 DASUCY010000000
VERSION     DASUCY010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900416"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..119)
                     /locus_tag="VFV25_00930"
     CDS             complement(<1..119)
                     /locus_tag="VFV25_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HEY1391908.1"
                     /translation="MSPRPVVLVTGAAKRLGREIALELARAGCDLALHYRHSEA"
     gene            214..1278
                     /locus_tag="VFV25_00935"
     CDS             214..1278
                     /locus_tag="VFV25_00935"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY1391909.1"
                     /translation="MAALIRREIAAAGGWLGFERYMELALYAPGLGYYAHGSRKFGVL
                     PQSGSDFVTAPELSPLFGRALARQVAQALEASGCDEVWEFGAGSGALADQLLGALGER
                     VRCYTIVDLSGSLRERQRARLAAHAHKLAWADRLPQAFSGVVVGNEVLDAMPVALLHF
                     DGGQWRERGVADGGAGFAWADRPTELRPPVDTGFVPGAVTELHRQAEAFIATLAAALQ
                     RGAAFFVDYGFPEAEYYHPQRIGGTLMCHRGHQADTDPLSDVGDKDITAHVNFTGIAL
                     AGQEAGLDVLGYTSQARFLLNCGLAGDLEAAELPVRINALRLINEHEMGELFKVVGFG
                     KDLPHFEALGFTQGDRSHTL"
     gene            1305..1970
                     /gene="lepB"
                     /locus_tag="VFV25_00940"
     CDS             1305..1970
                     /gene="lepB"
                     /locus_tag="VFV25_00940"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEY1391910.1"
                     /translation="MKQWLRDNRGFLIFLLLFGLFRTAVADWNPIPSGSMRPTLREGD
                     VVFVNRLAYQLKVPLTDVVLMRTGEVRRGDIVTFSSPSDGTRLIKRVIGLPGDVVKMR
                     NKVLFINGQRADYLREKALLEPVGAQQVPARQMRESIAGLKHEMQWFDGRAGAPDFGP
                     LRVPADHYLMLGDNRDDSADSRYFGLVARNKLIGRAERVLVSVDMLNGWRLQPSRFGL
                     ALR"
     gene            complement(1944..3188)
                     /locus_tag="VFV25_00945"
     CDS             complement(1944..3188)
                     /locus_tag="VFV25_00945"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HEY1391911.1"
                     /translation="MQIYKVGGAVRDALLGLPVQDHDWVVVGATPEAMVARGYLPVGK
                     DFPVFLHPKTREEYALARTERKTARGYHGFSFVTAPEVTLEQDLARRDLTINAMAEDA
                     QGRLIDPYHGQRDLEQRVLRHVSEAFVEDPVRILRLARFAARFADFSVAPETQALMRH
                     MVEDGEVDALVSERVWQELSRGLMEKHPSRMFEVLRGSGALARLLPEVDRLWGVPQRA
                     DYHPEVDTGVHLMMVLDMSARLDAPLAVRYACLGHDLGKGTTPAEILPRHLGHEERSV
                     RLLREMSERLRAPNECRELAEVVAREHGNVHRSLEIGAAALLRLLERCDAFRKPQRFD
                     DILLACECDARGRLGYEDGDYPQRARLLQALQLALTVDTAEVAAQAQQRGAQGPQIAR
                     AIHEARAQALSRQALTQGQPEA"
     gene            complement(3250..3732)
                     /locus_tag="VFV25_00950"
     CDS             complement(3250..3732)
                     /locus_tag="VFV25_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY1391912.1"
                     /translation="MTSPQLDALDWRILDQLQQDASLSNQALAERVHVSPATCLRRVK
                     RLVDSGVIEKRIAILSPGAVGAGLTAVVEITLDHQAAERLSGFEARAAADPAVQQCYR
                     TSSGPDFVLIAQVRDMEAYHALAHRLLTSDANVRNVKTYFSVKRSKFAPALALPGPAA
                     "
BASE COUNT          611 a         1308 c         1328 g          669 t
ORIGIN      
        1 gcctcggaat gccgatagtg caaggccagg tcgcagccgg cgcgcgccag ttcgagcgcg
       61 atctcgcgcc cgagccgctt ggcggcaccg gtgacaagga cgacggggcg ggggctcact
      121 tcctacaatg gcgcggtgaa agaacaagtg ggaagtttag cggcgcgccc tggggagcag
      181 actccaggac acaacggtgc gactgacgcc gcgctggcgg cgctgatccg gcgcgaaatc
      241 gccgccgcgg gcggctggct gggcttcgag cgctacatgg agctggcgct ctatgcgccc
      301 gggctgggct attacgccca cggcagccgc aagttcggcg tcttgccgca gtcgggcagc
      361 gatttcgtca ccgcgcccga gctgagcccg ctgttcggtc gcgcgctggc ccgccaggtc
      421 gcccaggccc tggaggccag cggctgcgac gaagtctggg aattcggcgc cggctctggc
      481 gccttggccg atcaactgct tggcgctttg ggcgagcgcg tgcgttgcta caccatcgtc
      541 gatctctcgg gcagcctgcg cgagcgccag cgtgcgcggc tggccgccca tgcacacaag
      601 ctggcctggg cggaccggct gccgcaggcc ttcagcggcg ttgtggtcgg caacgaggtg
      661 ctggatgcca tgcctgtggc gctgctgcat ttcgacggcg ggcagtggcg ggagcgtggc
      721 gtcgccgatg gcggcgcggg cttcgcctgg gcggaccgcc ccacggagct gcgcccgccg
      781 gttgacacgg gcttcgtgcc cggcgccgtc accgagctgc accgccaggc cgaggccttc
      841 atcgcgacgc tggccgcggc cctgcagcgc ggtgccgcct tcttcgtcga ctacggcttt
      901 cccgaggccg agtactacca cccgcagcgc atcggcggca cgctgatgtg ccatcgcggc
      961 caccaggccg acaccgatcc gctaagcgac gtgggcgaca aggacatcac cgcgcatgtg
     1021 aatttcaccg gcatcgcctt ggccggccag gaggccgggc tggacgtgct gggctatacg
     1081 agtcaggcgc gctttttgtt gaactgcggc ctggcgggcg atctcgaagc cgccgagctg
     1141 ccggtgcgga tcaatgcctt gcggctcatc aacgagcacg agatgggtga gctgttcaag
     1201 gtggtcggtt tcggcaagga cctgccccat ttcgaggccc tcgggtttac gcagggcgac
     1261 cgcagccata ccctttaggc tgcgcgcaag ctggagacac atcgatgaag caatggctgc
     1321 gtgacaaccg gggctttctg atctttctgc tgctgttcgg gctgttccgc accgcggtgg
     1381 ccgactggaa cccgatcccc tcaggctcga tgcgtcccac gctgcgcgaa ggcgacgtag
     1441 tcttcgtcaa ccgtctggcc tatcagctga aagtaccgct gaccgacgtg gtgctgatgc
     1501 gcaccggcga agtccggcgc ggcgacatcg tcaccttctc gtcgccctcc gacggcacgc
     1561 ggctcatcaa gcgggtgatc ggcctgcccg gcgacgtcgt aaagatgcgc aacaaggtgc
     1621 tcttcatcaa cgggcaacgg gccgactact tgcgcgagaa ggcactgctc gaacccgtgg
     1681 gtgcgcagca ggtgccggct cggcaaatgc gcgaatcgat cgccgggctg aagcacgaga
     1741 tgcaatggtt cgacggccgc gccggcgcgc ccgacttcgg cccgttgcgc gtgccggccg
     1801 accattacct gatgctgggc gacaaccgcg acgacagcgc cgactcgcgt tatttcggcc
     1861 tggttgcgcg caacaagctg atcggtcgcg ccgaacgcgt gctcgtctcg gtggatatgc
     1921 tcaatggctg gcggctgcag ccatcacgct tcgggttggc cttgcgttag ggcctgccga
     1981 ctcaaggcct gcgcccgcgc ctcgtggatc gctcgcgcga tctgcggccc ctgagctcca
     2041 cgctgctggg cctgagccgc cacttcggcg gtgtccacgg tcagcgcgag ctgcaaggct
     2101 tgcagtaggc gcgcccgttg cgggtagtcg ccgtcctcat agcccaggcg cccgcgcgca
     2161 tcgcattcgc aggccagcag gatgtcgtcg aagcgctgcg gcttgcggaa ggcgtcgcag
     2221 cgctccagca ggcgcagcag agcggcggca ccgatttcca ggctgcggtg cacgttgccg
     2281 tgctcgcgcg ccacgacctc ggccaactcg cggcactcgt tgggcgcgcg caggcgctcg
     2341 ctcatttcgc gcagcaggcg cacgctgcgc tcctcatggc ccagatggcg cggcagtatt
     2401 tccgccggcg tcgtgccctt gcccaggtcg tgccccaggc aggcatagcg cacggccagc
     2461 ggcgcatcca gcctggcgct catgtccagc accatcatca ggtgcacacc ggtgtcgacc
     2521 tcggggtggt agtcggcgcg ctgaggcacg ccccacagac gatcgacctc gggcagcagc
     2581 cgcgccagcg caccgctgcc gcgcagcacc tcgaacatgc gcgaagggtg cttttccatc
     2641 aggccgcgcg acagctcctg ccacacacgc tcgctcacca gggcgtcgac ctcgccgtct
     2701 tccaccatat ggcgcatcag cgcctgcgtc tcgggcgcca ccgagaagtc cgcaaagcgt
     2761 gcggcgaagc gcgctaggcg caggatgcgc accggatctt cgacgaaggc ctcggacaca
     2821 tggcgcagca cgcgctgctc gaggtcgcgc tgaccgtggt aagggtcgat gagccgcccc
     2881 tgcgcgtcct cggccatcgc gttgatcgtg agatcgcggc gcgccaggtc ctgctccagc
     2941 gtcacctcgg gtgcggtgac gaagctgaag ccgtggtagc cgcgcgccgt cttgcgctcg
     3001 gtgcgcgcca gcgcatattc ctcgcgcgtt ttcggatgca ggaagaccgg aaagtctttg
     3061 cccaccggca gatagccccg cgccaccatg gcttcgggcg tggcgccgac gacgacccag
     3121 tcgtgatcct gcaccggcag acctaggagg gcatcacgta ctgcgccgcc gactttgtag
     3181 atctgcatgg agggagtgta gttaaggggg ctgcatggct gcgcacggca cgtcgtgcgc
     3241 agggcggtct tacgcagcag gcccgggcag cgccagcgcc ggtgcgaact tgctgcgctt
     3301 gaccgagaaa taggtcttca cgttgcgcac attggcatcg ctcgttaaca ggcgatgcgc
     3361 cagcgcgtga taggcctcca tgtcgcgcac ctgcgcgatg agcacgaaat ccggcccgct
     3421 ggacgtgcgg tagcactgct gcaccgccgg gtcggccgct gcacgcgcct cgaagccgct
     3481 cagcctctct gcggcctggt ggtccagcgt gatctcgacc accgcggtga gccccgcgcc
     3541 cacggctccg ggcgagagga tggcgatgcg cttttcgatc acgccggagt ccaccagccg
     3601 cttgacgcgc cgcaggcagg tggccggcga gacgtgaacg cgttcggcca gggcttggtt
     3661 gctcagcgag gcgtcttgct gcagctggtc gaggatgcgc caatcgaggg catccagttg
     3721 cggggatgtc atgactgatt atttcaagaa cttctgcggt tagaaatttt agatcggcat
     3781 tgatgaatga agatcgaata gcgcgcatca gatgatagat tgcaatttaa tttcatacgg
     3841 ccctgtctac catcctggca ttccaagctc ggccggagac ttcttccatg tgcggcatcg
     3901 tcggcgctgt cagcca
//
LOCUS       DASUCY010000048         6955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_482740, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000048 DASUCY010000000
VERSION     DASUCY010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 6955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6955
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482740"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VFV25_00955"
     CDS             <1..493
                     /locus_tag="VFV25_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEY1391913.1"
                     /translation="QNWRWAGVPFYLRTGKRLAARDAQIVVNFRPVPHPIFPGANRPN
                     KLVIKLQPEDGLELHLSAAKGTGQTEALSPVFLDLDFDKAFSTARVGAYERLLLDAIA
                     GRLNLFVRSDEQEQAWRWVEPVLDAWQRDSSGPRIYAAGSWGPAAASALVARDGFAWS
                     EEQ"
     gene            511..1365
                     /locus_tag="VFV25_00960"
     CDS             511..1365
                     /locus_tag="VFV25_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEY1391914.1"
                     /translation="MLDRIRASIPALPPAEQRVAKLVLADPRSFASLPVVELAERAHV
                     SKPTVVRFCRSVGYDGLADFKLKLAGAVNEGVPFVHRAVDVDDKPGDLIVKVIDNTVS
                     ALLKFRNDAAGHAFERAIAALTEAGKSGRKIEFYGVGNSGIVAQDAQHKFFRLGVNAV
                     AYSDGHVQVMAATMLGPGDCAVVISNSGRSRDLLDASEIARKNGATTIVITASGSPLA
                     HQAQILLAADHPEDYDRYSPMVSRLLHLMIIDILTTGVALRLGEKLRPVLQEIKKNLR
                     AKRYAQGE"
     gene            complement(1391..1672)
                     /locus_tag="VFV25_00965"
     CDS             complement(1391..1672)
                     /locus_tag="VFV25_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY1391915.1"
                     /translation="MLLLPETLTAREARDTLRMLTTALEKEAKASAAELVVDGSPLQR
                     FDSSALAVLLECQRQTQAWGKRFAVKHMPQQLAELAKLYGATDLLPTAA"
     gene            complement(1699..2328)
                     /locus_tag="VFV25_00970"
     CDS             complement(1699..2328)
                     /locus_tag="VFV25_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY1391916.1"
                     /translation="MMLKRLLSFIAFAALALSSGFAAAETTTPDGMIKQLSNEVIDTV
                     KSDKAIQAGDIKKIMDLVDAKVLPHVNFQRMTAAAVGRYWRQATPDQQQRLQNEFKVL
                     LVRTYSGALTQVRDQVVELKPMRSKPEDTEVVVRTEVKGSGDPIQLDYRLEKAGNEWR
                     IYDINVLGVWLVENYRNSFAQEISANGIDGLINKLAEKNKAAAVATKKS"
     gene            complement(2460..3269)
                     /locus_tag="VFV25_00975"
     CDS             complement(2460..3269)
                     /locus_tag="VFV25_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEY1391917.1"
                     /translation="MRWSSRSAALALTLLLSACATASRNPQDPLEPWNRKVFAFNESV
                     DEHVLKPAATVYSNVVPRPVRTAADNFFGNVKDVWSAINLMLQGRFADGAHDAMRFGA
                     NSVFGFFGIADVATEMGLERQNEDFGQTLGKWGVPPGAYIVWPILGSSTVRDSVGLPI
                     DLKASPESFVKHVPARNTMLAVRTVNARADLLGATKLLDEIVLDKYTFIRDAYLQRRR
                     SLIYDGEPPDDDSSKDWSKPDSPAPAAPAASQPADGKDKTEDSPADTSGHS"
     gene            complement(3266..3763)
                     /gene="mlaD"
                     /locus_tag="VFV25_00980"
     CDS             complement(3266..3763)
                     /gene="mlaD"
                     /locus_tag="VFV25_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEY1391918.1"
                     /translation="MSKKGLESLVGFFVLLGIMGVVFLALKAANLASFSRADTYSVTA
                     RFDNIGGLKVRAPVRSAGVTVGRVTRISLDGKTYQGVVTLDIDRGYEFPKDSSAKILT
                     AGLLGDQYIGLEPGADEKNLVPGDTIKQTQSAVVLENLIGQLLFNKAAEAGSESNKGG
                     AESKK"
     gene            complement(3786..4568)
                     /gene="mlaE"
                     /locus_tag="VFV25_00985"
     CDS             complement(3786..4568)
                     /gene="mlaE"
                     /locus_tag="VFV25_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HEY1391919.1"
                     /translation="MTGLLAYLGNRTLHVLRSFGHAGGFFVDLLRYSPAAMRRFGLVV
                     AQIYAIGNLSLLIILASGLAVGFVLALQMYYALVTYGATESLGLIVNLALVRELGPVV
                     AALLFAGRAGTSLTAEIGLMKAGEQLAAMELMAIDPRSRVLAPRFIGGILSMPLLAAL
                     FSAIGILGAYVVAVLLIGIDSGNFWSIMQERVDVWRDVGNGIVKSAVFGVICTFVALY
                     QGYETQATPEGVAHATTRTVVISSLSVLGMDFILTALMFSTP"
     gene            complement(4565..5377)
                     /locus_tag="VFV25_00990"
     CDS             complement(4565..5377)
                     /locus_tag="VFV25_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY1391920.1"
                     /translation="MKSDNLIEIDHVTFGYDASRTILNDVSLSFPRGKVTAILGGSGC
                     GKTTLLRLIGGVYGVNQGRVVFDGEVVDAQDKEQLFKLRRRLGMLFQFGALFTDLTVF
                     ENVAFPLREHTDLPEDMIRDIVLMKLNAVGLRGAASLRISQVSGGMARRIALARAIAL
                     DPELIMYDEPFAGLDPISMGVTANLIRKLNDATGSTTLMVSHDVQECFSICDYAYLMS
                     TQGRVLVHGRPEELNACEDPEVRQFIRGEPDGPVRFHYPAKPLAEELGLRAS"
     gene            complement(5597..>6955)
                     /locus_tag="VFV25_00995"
     CDS             complement(5597..>6955)
                     /locus_tag="VFV25_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEY1391921.1"
                     /translation="EEAKIQGARCMDCGTPFCNSGCPVNNIIPDFNDLVYRDDWANAI
                     RVLHSTNNFPEFTGRICPAPCEAACTLNVNDDPVGIKSIEHAIIDRAWEEGWVQPQPP
                     KARTGKRVAVVGSGPAGLAAAQQLARAGHDVTVFEKNDRIGGLLRYGIPDFKMEKSHI
                     DRRVQQMQAEGVSFRTGVLVGHLPEGSKITNWSKEIVSADQLKAEFDAVLLAGGAEAS
                     RDLPAPGRDLDGVHFAMEFLPLQNKLNAGDKVKGQIRADAKHVIVIGGGDTGSDCVGT
                     SNRHGAASVTQFELLPMPPEQENKELTWPYWPYKLRTSSSHEEGCEREFAIATKEFIG
                     EKGKLTALKTVRVEWKNGKMSEVPGSEQTLKADLVLLAMGFVSPVGTVLDAFGVEKDQ
                     RGNAKASTEFFGGYKTSADKVFAAGDIRRGQSLVVWAIREGRQAARAVDEFLMGSSEL
                     PR"
BASE COUNT         1184 a         2300 c         2247 g         1224 t
ORIGIN      
        1 gcagaactgg cgctgggccg gcgtgccctt ctatctgcgc accggcaagc gcctggcggc
       61 acgcgacgcg cagatcgtcg tcaatttccg gcccgtgccg caccccatct tcccgggcgc
      121 gaatcggccc aacaagctgg tcatcaagct ccagcccgag gatggcctgg aactgcacct
      181 gtcggcagcc aagggcaccg gccagaccga ggcgctgtcg ccggtctttc tggacctcga
      241 tttcgacaag gctttcagca ccgcgcgcgt cggcgcctac gagcgcttgc tgctcgatgc
      301 aatcgccggg cggctcaacc tcttcgtgcg cagcgacgag caagagcagg cctggcgctg
      361 ggtcgagccc gtcctcgacg cctggcagcg cgacagcagc gggccgcgca tctatgccgc
      421 gggctcctgg ggcccggccg ccgccagcgc gctggtggcg cgcgacggct tcgcttggtc
      481 cgaagaacaa taggctccag aagcttggcc atgctggacc ggatacgcgc ctccatcccc
      541 gccctgccgc cggccgagca gcgcgtagcc aagctggtgc tggccgatcc gcgcagcttc
      601 gccagcctgc ctgtggtcga gctggctgaa cgcgcccatg tcagcaagcc cacggtcgtg
      661 cgcttttgcc gcagcgtggg ctatgacggg ctggccgact tcaagctcaa gctggccggc
      721 gcggtcaacg agggcgtgcc cttcgtgcat cgcgcggtcg acgtggacga caagccgggc
      781 gatctcatcg tcaaggtcat cgacaacacg gtctcggcgc tgctcaagtt ccgcaacgac
      841 gcggccggcc acgccttcga gcgcgccatt gcggcgctga ccgaggccgg caagagcggc
      901 cgcaagatcg agttctacgg cgtgggcaac tcgggcatcg tggcccagga cgcgcagcac
      961 aagttctttc gcctgggcgt caatgcggtg gcctacagcg acggccatgt gcaggtgatg
     1021 gccgccacca tgctgggccc cggtgactgc gccgtggtca tttccaactc aggccgcagc
     1081 cgcgacctgc tggacgccag cgagatcgcc cgcaagaacg gcgcgaccac catcgtcatc
     1141 acggcgagcg gctcgccgct cgcccaccag gcgcagatcc tgctggcagc cgaccaccct
     1201 gaggactacg accgctacag cccgatggtg tcgcgcttgc tgcacctgat gatcatcgac
     1261 atcctgacca ccggcgtggc gctgcgcctg ggcgagaagc tgcgcccggt gctgcaggag
     1321 atcaagaaga atttgcgcgc caagcgctac gcgcagggcg agtgaggagc gctcactcgc
     1381 ctgcgtgggg tcaggctgcg gtcggcagga gatcggtcgc gccgtagagc ttggccagct
     1441 cggccagctg ctgcggcatg tgcttcaccg cgaagcgttt gccccaggcc tgggtttggc
     1501 gctggcactc cagcagcacg gccagggcgc tggaatcgaa acgctgcagc ggcgagccgt
     1561 cgacgaccag ctcggccgcg ctcgccttgg cttccttttc cagggcggtc gtcagcatgc
     1621 gcagcgtgtc gcgcgcctcg cgggccgtca gcgtttcggg caagagcagc atggtggcac
     1681 gcctccagcg gtcagtaatt acgacttctt ggtggccacc gcggcggcct tgttcttctc
     1741 ggccagcttg ttgatcaggc catcgatgcc attggcgctg atttcctgcg cgaagctgtt
     1801 gcgatagttc tcgaccagcc agacgcccag cacgttgatg tcatagatgc gccactcgtt
     1861 gccggccttc tcgagccggt agtcgagctg gatcgggtcg ccgctgccct tgacctcggt
     1921 gcgcacgacc acttccgtgt cttcgggctt ggagcgcatg ggcttgagct cgaccacctg
     1981 gtcgcgcacc tgggtcagcg caccggaata agtgcgcacc agcaggacct tgaactcgtt
     2041 ctgcaggcgc tgctgctggt ccggcgtagc ctgacgccag tagcggccga ctgccgcggc
     2101 ggtcatgcgc tggaagttca cgtgcggcag caccttggcg tccaccaggt ccatgatctt
     2161 cttgatgtcg ccggcctgga tggccttgtc ggacttcacc gtgtcaatca cttcgttgga
     2221 cagctgcttg atcatgccgt ccggcgtggt ggtctcggcc gcagcaaaac cgctggacaa
     2281 ggccagggcc gcaaaggcga tgaaggagag gagtcgtttc agcatcatgg tcctttctag
     2341 aagcccatcg ccacccacat gtggcgccgg gcttgaagcc tacaacgtcg cgaaccaccc
     2401 tgcggttcac cggaacgttt gcaggcgaac actaacttat ctgccggtcc gtactatttc
     2461 taggaatgac cagatgtatc tgctggtgaa tcttcggttt tgtctttccc gtcggccggc
     2521 tggctggctg cgggagcggc aggggccggg ctgtcgggct tgctccagtc cttggagctg
     2581 tcgtcgtcag gcggctcgcc gtcatagatc aggctgcgtc ggcgctgcag ataagcgtca
     2641 cgtatgaagg tgtacttgtc gagcacgatc tcgtcgagca gcttggttgc gcccagcagg
     2701 tcggcccgcg cgttgaccgt gcgcacggcc agcatggtgt tgcgcgccgg cacatgcttg
     2761 acgaaggact cgggcgaggc cttgaggtcg atgggcaggc cgaccgagtc acgaaccgtc
     2821 gatgatccca gtatcggcca gacgatgtag gcgccgggcg gtacgcccca tttgcccagg
     2881 gtctggccga aatcctcgtt ctggcgctcc aggcccatct cggtggcgac gtcggcgatg
     2941 ccgaagaagc cgaacacgct gttggcaccg aagcgcatcg catcgtgcgc tccgtctgcg
     3001 aagcggccct gcagcatcag attgatcgcc gaccagacgt ccttcacatt gccgaagaaa
     3061 ttgtcggcag ccgtgcgcac cgggcgcggc acgacgttgc tgtagaccgt ggcagccggc
     3121 ttgagcacat gctcatcgac cgactcgttg aaggcgaaga ccttgcggtt ccagggctcc
     3181 agcggatcct gcggattccg gctggctgtg gcacaggcgc tcagcagcag cgtcagggcc
     3241 agcgcggcgc tgcgcgacga ccacctcact tcttgctctc cgcgccgccc ttgttgcttt
     3301 cactgccggc ttcggcggcc ttgttgaaga ggagctggcc gatcaggttc tccagcacca
     3361 cggcggactg agtctgcttg atcgtgtcgc cgggcacgag gttcttctca tcggcgccgg
     3421 gctcgaggcc gatgtactgg tcgcccagca ggcccgcggt caggatcttg gccgaggaat
     3481 ccttcgggaa ttcgtagccg cggtcgatgt ccagcgtcac gacgccctgg taggtcttgc
     3541 cgtccagcga gatcctcgtg acgcggccca ccgtgacgcc ggcgctgcgc accggggcgc
     3601 gcaccttgag gccgccgatg ttgtcgaaac gcgccgtcac gctataggtg tcggcgcgcg
     3661 agaagctggc caggttggct gccttcaggg ccaggaagac cacgcccatg atgcccagca
     3721 gcacaaaaaa accgaccaga gattcgagac ccttcttgct catatgccta tgctccagct
     3781 tctcatcaag gcgtcgagaa catcagcgcc gtgagaatga aatccatacc gagtacgctc
     3841 aaggacgaaa tcacgacggt gcgtgtcgtc gcgtgggcca cgccttctgg ggtggcctgg
     3901 gtttcatagc cctggtacag ggccacgaag gtgcagatga cgccgaacac ggcgctcttg
     3961 acgatgccgt tgccgacatc gcgccagacg tcgacgcgct cctgcatgat cgaccagaag
     4021 ttgcccgaat cgatgccgat cagcagcacc gccacgacat aggcgccgag gatgccgatg
     4081 gcggaaaaca gcgctgcgag cagcggcatc gacaagatgc caccgataaa gcgcggcgcc
     4141 agcacgcggc tgcgcgggtc gatggccatc agctccatcg ccgccagctg ctcgcccgcc
     4201 ttcatcaggc cgatctcggc ggtcagcgag gtgccggcgc gccccgcaaa gagcagcgct
     4261 gccaccaccg gccccagctc gcgcaccagt gccagattga cgatcaggcc cagcgattcg
     4321 gtcgcgccat aggtgaccag cgcgtaatac atctgcagcg ccagcacgaa gcccaccgcc
     4381 aggcccgacg ccaggatgat gagcagcgag aggttgccga tggcatagat ctgcgccacg
     4441 acgaggccga agcggcgcat cgcggcaggc gaatagcgca gcaaatcgac gaagaagccg
     4501 ccggcatggc cgaaggagcg cagcacatgc agcgtgcgat tgcctagata agcaagcagg
     4561 cccgtcatga tgcgcgcagc cccagttcct cggccagcgg cttggccgga tagtggaaac
     4621 gcaccgggcc gtcgggctcg ccgcgaatga actggcgcac ttcgggatcc tcgcaggcat
     4681 tgagctcttc cggacgcccg tgcaccagca cccgcccctg cgtcgacatc aggtacgcgt
     4741 agtcacagat cgagaagcac tcctgcacgt cgtgcgagac catcagcgtg gtggagccgg
     4801 tggcatcgtt gagcttgcgt atcagattgg ccgtgacgcc catcgagatc gggtcgagcc
     4861 ccgcgaaggg ttcgtcgtac atgatcagct cggggtcgag ggcgatcgcg cgggccagcg
     4921 caatgcgccg cgccatgccg cccgagacct gagagatgcg cagcgacgct gcgccgcgca
     4981 ggccaacggc attgagcttc atcagcacga tgtcgcggat catgtcctcg ggcaggtcgg
     5041 tgtgctcgcg caaggggaag gccacgttct cgaacaccgt gaggtcggtg aacagcgcgc
     5101 cgaactggaa cagcatgccc aggcgccgcc gcagcttgaa cagctgctcc ttgtcctgcg
     5161 catcgaccac ttcgccgtcg aacacgacgc ggccctggtt gacgccgtag acgccgccga
     5221 tcaggcgcag cagcgtggtc ttgccgcagc ctgagccgcc caggatggcc gtgaccttgc
     5281 ctcgcgggaa gctcaggctc acgtcattca ggatcgtacg gctcgcgtcg tacccgaagg
     5341 tgacatggtc gatctctatg aggttgtctg atttcaatcg cgaaagcgcc gctgcgggcg
     5401 catgcttgtt gttggtcact gccgcaaagc tggcagtatg agggatagcc ctaggctgga
     5461 aattgtcgca tgcctgtcgc aaagccgctg gcagcctaga aagcaggcaa aacagctgac
     5521 tcgcgaggtc cagtccgtta tcaaaaaacg acaaagccgc cctgaggcgg cccgtcgtcg
     5581 aagcccgcgc ggcggctcaa cgcggcagct cgctcgagcc catcaagaat tcgtcgaccg
     5641 cacgagcagc ctgccgaccc tctcggatcg cccacaccac gagtgactgg ccgcgacgga
     5701 tgtcgccggc ggcgaacacc ttgtcggcac tggtcttgta gccgccgaag aactcggtgc
     5761 tggccttggc attgccgcgc tggtcctttt cgaccccgaa ggcgtcgagc accgtgccca
     5821 ccgggctgac gaagcccatg gccagcagca cgagatcggc cttgagcgtc tgctcgctgc
     5881 ccggcacctc gctcatcttg ccgttcttcc attccacgcg caccgtcttc agcgccgtga
     5941 gcttgccctt ttcgccgatg aattccttgg tggcgatggc gaactcgcgc tcgcagccct
     6001 cctcgtggct cgatgaggtg cgtagcttgt agggccagta tggccaggtc agctccttgt
     6061 tctcttgctc gggcggcatc ggcagcagtt cgaactgggt gacgctcgca gcgccgtggc
     6121 ggttgctggt gcctacacag tcggatccgg tgtcgccgcc gccgatcacg atcacatgct
     6181 tggcatccgc gcggatctgg cccttgacct tgtcgccggc attgagcttg ttctgcagcg
     6241 gcaggaactc catcgcgaaa tgcacaccgt ccagatcacg gccaggcgcc ggcagatcgc
     6301 gcgaagcttc ggcgccaccc gccagcagca ccgcatcgaa ctcagccttg agctggtcgg
     6361 ccgacacgat ctccttggac caattggtga tcttgctgcc ctcgggcaga tgccccacca
     6421 gcacgccggt gcggaagctg acgccctcgg cctgcatctg ctggacgcgg cggtcgatgt
     6481 gcgacttctc catcttgaag tcgggaatgc cgtagcgcag caggccgccg atgcggtcgt
     6541 tcttctcgaa caccgtcacg tcatggccgg cacgcgccag ctgctgcgcc gcggccagtc
     6601 cagccgggcc ggagccgacg acggccacgc gcttgccggt cctggccttg ggcggctgcg
     6661 gctgcaccca gccttcttcc caggcgcggt cgatgatcgc gtgctcgatg gacttgatgc
     6721 ccaccggatc gtcgttgacg ttcagcgtgc aggccgcctc gcagggcgcg gggcagatgc
     6781 ggccggtgaa ttccggaaag ttgttggtcg agtgaagcac ccgtatcgca ttggcccagt
     6841 cgtcgcgata cacgaggtcg ttgaagtccg ggatgatgtt gttgaccggg cagccgctgt
     6901 tgcaaaaggg cgtgccgcag tccatgcaac gggcaccctg gatcttcgcc tcctc
//
LOCUS       DASUCY010000049         2075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1237793, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000049 DASUCY010000000
VERSION     DASUCY010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237793"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VFV25_01000"
     CDS             <1..263
                     /locus_tag="VFV25_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011350958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HEY1391922.1"
                     /translation="ADFNNRAKGGIPDDIPEVSLAGAPLGIGALLKQAGLVPSTSEGL
                     RMVEQGGVKIDGATVSDKGLKLEAGRFVVQVGKRKFAKVTLS"
     gene            344..1249
                     /locus_tag="VFV25_01005"
     CDS             344..1249
                     /locus_tag="VFV25_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEY1391923.1"
                     /translation="MQVSNYLKLSVVAAIVTIVLKTAAWWVTDSVGLLSDALESFVNL
                     AGAVFALAMVTISQRPADDDHPYGHHKAEYFSSGFEGVLIFGAAVAIIWAAAQRFFEP
                     QPLQSLGLGLVLAIISTIVNGVLGFGMLRTAKAHRSIALEADARHLFADVWTTVGVVL
                     GLTVVHFTGLLWLDALVGIGVALNILREGVSLVWRSVEGLMDHAVEPAVREQIDRALQ
                     PYAQPPVRFDHVVTRRAGRRRFVDLHMHVPAAWSLGQAAALRASVEQALMSAVPGLRP
                     SIQLLPTDVEAKFVADEEDERVQAS"
     gene            1246..1683
                     /gene="dtd"
                     /locus_tag="VFV25_01010"
     CDS             1246..1683
                     /gene="dtd"
                     /locus_tag="VFV25_01010"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEY1391924.1"
                     /translation="MIALVQRVAQARVEVAGRVVGAIGAGLLVLVCAEPGDTEAQADK
                     LVTKLLKLRIFSDASAKMNLSVQDVAGGLLVVSQFTLAADVSGGNRPSFTGAAAPELG
                     RRLYERVLAQARAQHPEVASGEFGADMQVHLVNDGPVTIPIRL"
     gene            complement(1706..1948)
                     /locus_tag="VFV25_01015"
     CDS             complement(1706..1948)
                     /locus_tag="VFV25_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HEY1391925.1"
                     /translation="MQPKIVVYSKSACAQCDMAKNLLKSRSLDFEEIRIDDEAQRLAF
                     YEKCGPAVRQMPQVFINDQRVGGVAGLQAALAQLGS"
BASE COUNT          299 a          706 c          713 g          357 t
ORIGIN      
        1 aggccgactt caacaaccgc gccaagggtg gcatcccgga cgacattccc gaagtgagcc
       61 ttgccggcgc gccgttgggc attggcgccc tgctcaagca ggccgggctc gtgccttcga
      121 ccagcgaggg cctgcgcatg gtcgagcagg gcggcgtcaa gatcgacggc gccacggtca
      181 gcgacaaggg gctcaagctc gaggccggcc gcttcgtcgt gcaggtgggc aagcgcaagt
      241 tcgcaaaggt cacgctgagc tgacgcagcc gccgggtgtg cccggcgggc gtcacagcca
      301 cgccgccggc ttcggctagg cttgcttcaa accttcaacg tacatgcaag tcagcaacta
      361 tctcaagctc tcggtggtgg cggccatcgt caccatcgtg ctcaagacgg cggcctggtg
      421 ggtcaccgat tcggtgggcc tgctgtccga tgcgctggag tccttcgtca atctcgccgg
      481 cgcggtattc gcgctggcca tggtgacgat ctcgcagcgc ccggccgacg acgaccaccc
      541 ttacggccat cacaaggccg agtacttctc cagcggcttc gagggcgtgc tgatcttcgg
      601 cgccgcggtg gccatcatct gggccgccgc gcagcgcttc ttcgagccgc agcctttgca
      661 gtcgctgggc ctgggcctgg tgctggcgat catcagcacg atcgtcaacg gcgtgctcgg
      721 cttcggcatg ctgcgcaccg ccaaggcaca ccgctcgatc gcgctggagg ccgatgcgcg
      781 gcatctgttc gccgacgtct ggaccacggt cggcgtggtg ctgggcctga ccgtcgtgca
      841 tttcaccggg ctgctatggc tcgacgccct ggtcggcatc ggcgtggcgc tcaacatcct
      901 gcgcgaaggc gtttcgctgg tctggcgctc ggtggaaggc ctgatggacc atgccgtgga
      961 gcctgccgtg cgcgagcaga tcgaccgcgc gctgcagccc tatgcgcagc cgccggtccg
     1021 cttcgatcac gtggtgacgc gccgcgccgg acgccgccgc ttcgtcgatc tgcacatgca
     1081 tgtgccggcc gcctggtcgc tcggccaggc cgctgcgctg cgcgcctcgg tcgagcaggc
     1141 cttgatgagt gccgtgcccg gcctgcgccc cagcatccag ctgctgccca ccgatgtcga
     1201 ggccaagttc gtcgccgacg aggaggacga gcgcgtgcag gcatcatgat cgcgctggtg
     1261 cagcgcgtgg cgcaggcgcg ggtcgaggtc gcgggccggg tggtcggtgc gataggcgcg
     1321 ggcctgctgg tgctggtctg tgccgagccc ggcgacaccg aggctcaggc cgacaagctg
     1381 gtgaccaagc tgctgaagct gcgcatcttc agcgacgctt cggccaagat gaacctgagc
     1441 gtgcaggacg tggccggcgg cctcctcgtc gtctcgcagt tcacgctggc ggccgatgtc
     1501 tccggcggca accgccccag cttcaccggc gccgctgccc ccgagctggg ccgcaggctc
     1561 tacgagcgcg tgctggcgca ggcccgcgcg cagcaccccg aggtcgcgag cggcgagttc
     1621 ggcgccgaca tgcaggtgca cctcgtcaac gacgggccgg tgacgatacc tatccggctc
     1681 tgagccgcag gcccggcccc cgaccctagc tgcccagctg agcgagagcc gcctgcaggc
     1741 cggcgacgcc gccgacgcgc tggtcgttaa tgaagacctg cggcatctgg cgcacggcag
     1801 ggccgcactt ctcgtagaac gccagccgct gcgcctcgtc atcgatcctg atttcctcga
     1861 aatcgagaga gcgggacttg agaaggttct tggccatgtc gcattgcgcg caggccgact
     1921 tcgagtagac gacgatcttg ggttgcatgg gcgagagttt attcaccggt gccacttatt
     1981 ggcggctggg gctttgctct cagcctgagg cgcaggccgc ggcccggtat gcgcaatcac
     2041 ttgtagctgc gcgcgtcctc gatcaccttg ccgtc
//
LOCUS       DASUCY010000050         7372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_193503, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000050 DASUCY010000000
VERSION     DASUCY010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 7372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7372
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193503"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1945
                     /gene="pnp"
                     /locus_tag="VFV25_01020"
     CDS             <1..1945
                     /gene="pnp"
                     /locus_tag="VFV25_01020"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEY1391926.1"
                     /translation="IEKTYAAGKIPGSFFKREGRPSELETLTSRLIDRPIRPLFPDGF
                     FNEVQVVVHVLSLNPEVEADIAALIASSAALAVSGIPFNGPIGAARVGYINGEYVLNP
                     SKTQQTQSRLDLVVAGTEAAVLMVESEADQLPEDVMLGAVVFGHDQGKIAIAAINELV
                     RDAGKPEWKWEAPSKDEGFIAKVSALGEDKLRAAYQIRSKQARTQACRDAYAAVKAGL
                     AEQGVEFDAVKVDNLLFEIEAKIVRSQILAGEPRIDGRDTRTVRPIEIRTGLLPRTHG
                     SALFTRGETQAVVVATLGTDRDAQRIDALSGEFEDRFMLHYNMPPFATGETGRVGSPK
                     RREIGHGRLAKRALVAALPSKEEFPYSIRVVSEITESNGSSSMASVCGGCLALMDAGV
                     PMKAHVAGIAMGLIKDSNRFAVLTDILGDEDHLGDMDFKVAGTTTGVTALQMDIKIQG
                     ITKEIMQVALAQAKEARMHILGKMQDAVSGAKTEVSQFAPRLYTMKINPEKIRDVIGK
                     GGATIRALTEETGTTIDIAEDGTITIASTDADKAAHAKKRIEEITAEVEVGKVYEGPV
                     TKILDFGALVNLLPGKDGLLHISQIAHQRVEKVTDFLSEGQIVRVKVLETDEKGRVKL
                     SMKALQERSADAGTAQQQQQQQQ"
     gene            2013..3002
                     /locus_tag="VFV25_01025"
     CDS             2013..3002
                     /locus_tag="VFV25_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003076506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEY1391927.1"
                     /translation="MQAIEISQPGGPEVLRLATRPDAVAGAGEALIRVTASGVNRPDV
                     LQRKGIYPPPPGASDLPGLEVAGEIVGGDAQALAQAGFGLGDHVCALVAGGGYAQLCA
                     APVGQCLPVPRGLSDVEAASLPETFFTVWSNVFERGRLQPGETLLVQGGSSGIGVTAI
                     QLAKALGSKVIVTAGDDAKCAACLRLGADHAINYKTEDFVAAVQRLTAGRGADVILDM
                     VAGAYVARELSCLADDGRLVFIAVQGGVKAEIDAGAVLRRRLTITGSTLRPRPVAFKA
                     AVAAALRAKVWPLIEAGQVRPVIQQVYAADQAAQAHAAMEASQHVGKLVLQWQ"
     gene            3017..3757
                     /gene="tpiA"
                     /locus_tag="VFV25_01030"
     CDS             3017..3757
                     /gene="tpiA"
                     /locus_tag="VFV25_01030"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEY1391928.1"
                     /translation="MRRKLVVGNWKMHGSHVGNAELLAGVLAAKPYQADVAVCVPFPY
                     LSEVAVALTGSHLLWGAQDVSAHEQGAYTGEVSATMLAEFGCRYAIVGHSERRAYHAE
                     GDQLVADKAKAALARGVTPIVCVGETLAQREAGETEAVVKRQLSAVIHALTHCIGEIV
                     VAYEPVWAIGTGKTATPDQAQEVHALLRTQLQAATPHAAGMRLLYGGSVKPDNAAALF
                     SQPDIDGGLIGGAALKAGDFIAICRAAA"
     gene            3801..4211
                     /gene="secG"
                     /locus_tag="VFV25_01035"
     CDS             3801..4211
                     /gene="secG"
                     /locus_tag="VFV25_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEY1391929.1"
                     /translation="MQALMTVMLVVQMLAALTMIGLVLVQHGKGADMGASFGSGASGS
                     LFGATGSANFLSRSTAVCATLFFVCTLTLAYMGNRRVEAPSTGSSVLDRAAAAPAASA
                     PALPQIPTAAASTVAAPAAPAAATASAPAQIPTK"
     gene            4446..4530
                     /locus_tag="VFV25_01040"
     tRNA            4446..4530
                     /locus_tag="VFV25_01040"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4480..4482,aa:Leu,seq:gag)
     gene            4751..5110
                     /locus_tag="VFV25_01045"
     CDS             4751..5110
                     /locus_tag="VFV25_01045"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEY1391930.1"
                     /translation="MLLEQYLPVILFILVGIGVGVAPQVLGFLLGPRRPDAEKNSPYE
                     CGFEAFEDARMKFDVRYYLVAILFILFDLEIAFLFPWAVTIREIGAAGFWAMMIFLGI
                     LVVGFAYEWKKGALDWE"
     gene            5126..5602
                     /locus_tag="VFV25_01050"
     CDS             5126..5602
                     /locus_tag="VFV25_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEY1391931.1"
                     /translation="MGIEGVLKEGFITTSADKLINWSKTGSLWPMTFGLACCAVEMMH
                     AGAARYDIDRFGMLFRPSPRQSDLMIVAGTLCNKMAPALRKVYDQMAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALLYGILQLQAKIRRENTIAR"
     gene            5616..6215
                     /locus_tag="VFV25_01055"
     CDS             5616..6215
                     /locus_tag="VFV25_01055"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEY1391932.1"
                     /translation="MNHKLDILQPALESALGSRIKSLKRDRGELTLIVAAADYAEVAR
                     TLRDHAELKFEQLIDLCGLDYSSYKNEAHEGLRFCVVSHLLSVSKNWRLRLKVFCPDD
                     ELPMVASLNEVWSSANWFEREAFDLYGIVFEGHEDLRRILTDYGFIGHPFRKDFPVSG
                     HVEMRYDPERKRVIYQPVTIEPREITPRVVREDNYGGLH"
     gene            6230..>7372
                     /locus_tag="VFV25_01060"
     CDS             6230..>7372
                     /locus_tag="VFV25_01060"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEY1391933.1"
                     /translation="MAEIKNYTLNFGPQHPAAHGVLRLVLELDGEVIQRADPHIGLLH
                     RATEKLAETRTFIQSLPYMDRLDYVSMMSNEHAYCLAIEKLLGIEVPLRAQYIRVMFD
                     EITRLLNHLLWLGAHGLDCGAMNILIYCFREREDLFDTYEAVSGARMHAAYYRPGGVY
                     RDLPDSMPQYKVSKIRNAKALAKMNENRQGSLLDFIDDFCRRVPKNVDDYETLLTDNR
                     IWKQRTVGIGVVSPERALNLGFTGPMLRGSGVEWDLRKKQPYEVYDRMDFDVPVGSNG
                     DCYDRYLVRVEEMRQSNRIIKQCVDWLRANPGPVITDNHKVAPPSRLDMKSSMEELIH
                     HFKLFTEGFHVPEGEAYAAVEHPKGEFGIYIVSDGANKPYRLKIRPP"
BASE COUNT         1298 a         2442 c         2438 g         1194 t
ORIGIN      
        1 catcgagaag acctatgccg ccggcaagat ccccggcagc ttcttcaagc gcgaaggccg
       61 ccccagcgag ctcgagacgc tgacctcgcg tctgatcgat cgcccgatcc gcccgctctt
      121 cccggacggc ttcttcaatg aggtgcaggt ggtcgtgcac gtgctgtcgc tcaaccccga
      181 ggtggaagcc gacatcgcgg ccctgatcgc ctccagcgcc gcgctggccg tctccggcat
      241 tcccttcaac ggcccgatcg gcgccgcgcg cgtgggctac atcaacggcg aatacgtgct
      301 gaacccgtcc aagacacagc agacgcagtc gcgcctggat ctggtcgtgg ccggcacaga
      361 agccgccgtg ctcatggtcg aatccgaggc cgatcagttg cccgaggacg tgatgctggg
      421 cgccgtggtg ttcggtcacg accagggcaa gatcgcgatc gccgcgatca acgagctggt
      481 gcgcgatgcc ggcaagcccg agtggaagtg ggaggctccg tccaaggacg agggcttcat
      541 cgccaaggtc agcgccctgg gcgaagacaa gctgcgcgcg gcctaccaga tccgctccaa
      601 gcaggcccgc acccaggcct gccgcgacgc ctatgcagcc gtgaaggcgg gcctggccga
      661 gcagggcgtg gagttcgacg cagtcaaggt cgacaacctg ctgttcgaga tcgaggccaa
      721 gatcgtgcgc agccagatcc tggccggcga gccgcgcatc gacggccgcg acacccgcac
      781 cgtgcgtccc atcgagatcc gcaccggcct gctgccccgc acccacggct cggcgctgtt
      841 cacgcgcggc gagacccagg cggtggtggt ggccacgctg ggcaccgacc gcgacgctca
      901 gcgcatcgat gcgctgtccg gcgagttcga agaccgcttc atgctccact acaacatgcc
      961 gcccttcgcc accggcgaaa cgggccgcgt gggcagcccc aagcgccgcg agatcggcca
     1021 tggccgcctg gccaagcgcg cgctggtcgc ggcactgccg agcaaggaag agttccccta
     1081 ttcgatccgc gtcgtctccg agatcaccga atccaacggc tcctcgtcga tggcctcggt
     1141 gtgcggcggc tgcctggcgc tgatggacgc cggcgtgccg atgaaggccc acgtggccgg
     1201 catcgcgatg ggcctgatca aggacagcaa ccgcttcgcg gtgctgaccg acatcctggg
     1261 cgacgaggac cacctgggcg acatggactt caaggtggcc ggcacgacca ccggcgtcac
     1321 cgcgctgcag atggacatca agatccaggg catcaccaag gaaatcatgc aggtcgcgct
     1381 ggcccaggcc aaggaagccc gcatgcacat cctcggcaag atgcaggacg cggtgagcgg
     1441 cgccaagacc gaggtctcgc agttcgcgcc gcgcctgtac acgatgaaga tcaacccgga
     1501 gaagatccgc gacgtgatcg gcaagggcgg cgccaccatc cgcgcgctga ccgaagagac
     1561 cggcacgacg atcgacatcg ccgaagacgg cacgatcacg atcgcctcga ccgacgccga
     1621 caaggcggcg cacgccaaga agcgcatcga ggagatcacc gccgaagtcg aggtgggcaa
     1681 ggtctacgaa ggcccggtca ccaagatcct ggactttggt gccctggtga acctgctgcc
     1741 cggcaaggac ggcctgctgc acatcagcca gatcgcccac cagcgcgtcg agaaagtcac
     1801 cgacttcctg agcgaaggcc agatcgtcag ggtcaaggtg ctcgagaccg acgagaaggg
     1861 ccgcgtcaag ctctcgatga aggcgctgca ggagcgcagt gccgatgctg ggactgctca
     1921 acaacaacaa cagcaacagc agtgagtagt gatctgtgac cggtgacttg tgaccggtca
     1981 ctagtcacga gtcactggtc actactgcac gcatgcaagc catcgaaata tcgcaacccg
     2041 gcgggcccga ggtgctgcgc ctggccacgc gccccgacgc ggtagcgggc gcgggcgagg
     2101 cgctgatccg cgtgaccgcg tccggcgtga accgcccgga cgtgctgcaa cgcaagggga
     2161 tctatccgcc gccgcccggt gcctcggatc ttccgggcct ggaggtggcg ggcgagatcg
     2221 tcggcggcga tgcgcaggcg ctggcccagg ccggcttcgg gctgggcgac catgtttgcg
     2281 cgctggtggc gggcggcggc tacgcgcagc tgtgcgcggc gcccgtcggc caatgcctgc
     2341 cggtgcccag gggcctgagc gatgtcgagg cggccagcct gccggagacc ttcttcacgg
     2401 tctggtccaa cgtcttcgag cgcggccgcc tgcagcccgg cgaaaccctg ctggtgcaag
     2461 gcggcagcag cggcatcggc gtcacggcca tccagctggc caaggcgctg ggttccaagg
     2521 tgatcgtgac ggccggcgac gacgccaaat gcgcggcctg cctgcggctc ggcgccgatc
     2581 acgcgatcaa ctacaagacc gaggacttcg tcgcggccgt gcagcgcctc accgcgggcc
     2641 gtggcgcgga tgtgatcctc gacatggtgg cgggtgccta cgtggctcgc gagctgagct
     2701 gcctggctga cgacggtcga ctcgtgttca tcgcggtgca ggggggtgtc aaggccgaga
     2761 tcgatgccgg tgccgtgctg cgccggcgcc tgacgatcac gggctcgacc ttgcgcccgc
     2821 gcccggtggc cttcaaggcc gccgtcgctg cggcgctgcg cgccaaggtg tggccgctga
     2881 tcgaagcggg gcaggtgcgg ccggtcattc agcaggtcta cgcggccgat caagccgcgc
     2941 aagcgcatgc cgccatggaa gcgagccagc acgtgggcaa gctggtgctg cagtggcaat
     3001 agcgggagga ggagagatgc gacgcaagct ggtggtgggc aactggaaga tgcatggcag
     3061 ccatgtcggc aacgccgagc tgctggccgg cgtgctggcc gccaagcctt atcaggccga
     3121 tgtcgcggtc tgcgtgcctt tcccctatct gtccgaagtg gcggttgcgc tgaccggcag
     3181 ccatctgctg tggggtgcac aggatgtttc ggcccatgag cagggcgcct acaccggcga
     3241 agtgtcggcc acgatgctgg ccgagttcgg ctgtcgctat gccatcgtcg gccactcgga
     3301 gcgccgcgcc tatcacgccg aaggcgacca gctcgtcgcc gacaaggcca aggcggcgct
     3361 ggcgcgtggc gtgacgccca tcgtctgcgt cggcgaaacc ctggcccagc gcgaggccgg
     3421 cgagaccgag gccgtcgtca agcgccagct gtcggccgtg atccacgcgc tgacgcattg
     3481 catcggcgaa atcgtcgtgg cctacgagcc ggtctgggcg atcggcaccg gcaagacggc
     3541 cacgcccgac caggcgcagg aagtgcatgc cctgctgcgc acccagctgc aggcggccac
     3601 gccgcacgcg gccggcatgc ggctgctcta cggcggcagc gtcaagcccg acaatgccgc
     3661 cgcgctgttc agccagcccg acatcgatgg cggcctgatc ggcggcgcgg cgctgaaggc
     3721 gggcgatttc atcgcgattt gccgtgccgc ggcttgaagc ccgccgcacg aacacttaga
     3781 gaaatacgga gaaggaaacc atgcaagctt tgatgacggt gatgttggtc gtgcagatgc
     3841 tggcggccct gacaatgatc ggcctggtgc tggtccagca cggcaagggg gccgacatgg
     3901 gcgcctcctt cggcagcggc gcttcgggca gcctgttcgg cgccaccggc agtgccaact
     3961 tcctgtcgcg ctcgacggcg gtgtgcgcca cgctgttctt tgtctgcacg ctgaccctgg
     4021 cctacatggg aaatcgccgc gtcgaggccc cgagcaccgg ctccagcgtg ctcgatcgcg
     4081 cggccgccgc gccggcggcc agcgcgcccg ccttgccgca gattcccacc gccgcggcca
     4141 gcacggtcgc cgctcccgca gcgcccgctg ctgcgacggc ttccgcgccc gcgcaaattc
     4201 ctaccaagta aagccgcgta tagcgcagcc tcccgctgga tatcacatgc cacctgcagt
     4261 cgcgccaaca tcactgcgcg atcgttgagt ggcccttgaa tcgggttaga attcaaggct
     4321 gctcgacgag caattcctca ggcgaagtga gcagcaaagt cacagtgttc ggttctggtg
     4381 cggtcctggt gttgagctgg gtcagtcaga tcggaaagat cggaaacggg tcctgtgaag
     4441 cgtatgccga cgtggtgaaa ttggtagaca cgctatcttg agggggtagt ggcgaaagct
     4501 gtgcgagttc gagtctcgcc gtcggcacca gacacaagag tttcatcggg ccgggttctc
     4561 atccggtaca cggcccgagc gccctgatgg caatgcttgg agcggtgcac agggtccgag
     4621 cgggcgcgct gagcgtggga ttggaggtgg gttcccgccc gatcactgca gcgcgctttg
     4681 ttttggccgg atggttttgc agacccagca gcgatttgtt gttcgaagtg cctgaccaga
     4741 gccagcaatg atccttcttg aacaatatct ccccgtcatc ttgttcatcc tggtcggcat
     4801 cggcgttggc gtggcacccc aggtgctggg cttcctgctc gggccgcgcc ggcccgacgc
     4861 ggaaaagaat tccccctacg aatgcggctt cgaggccttc gaagacgcgc gcatgaagtt
     4921 cgacgtgcgc tattacctgg tcgccatcct cttcatcctg ttcgacctgg aaattgcgtt
     4981 tcttttcccg tgggccgtga ccatccgcga aatcggcgcg gccggcttct gggccatgat
     5041 gatcttcctc ggcatcctcg tcgtgggctt cgcctacgaa tggaaaaagg gcgccctgga
     5101 ctgggaatga tgtgaggcaa gcggtatggg catagagggc gtccttaagg aaggtttcat
     5161 caccacgtcg gccgacaagc tgatcaactg gtccaagacc ggctcgctgt ggccgatgac
     5221 cttcggcctg gcctgctgcg cggtcgagat gatgcatgcg ggcgcggccc gctacgacat
     5281 cgaccgcttc ggcatgctgt tccgccccag cccgcgccag tccgacctga tgatcgtggc
     5341 cggcacgctg tgcaacaaga tggcgccggc gctgcgcaag gtctacgacc agatggccga
     5401 gccgcgctgg gtgctcagca tgggctcctg cgccaacggc ggcggctact accactacag
     5461 ctactcggtg gtgcgcggct gcgaccgcat cgtgccggtg gacgtctacg tgcccggctg
     5521 cccgccgacg gccgaggcgc tgctctacgg catcctgcag ctgcaggcca agatccggcg
     5581 cgaaaacacg atcgctcgct gagggccgca cgcgcatgaa ccataagctg gacattctcc
     5641 aacctgcgct cgagtccgca ctcggctcca ggatcaagag cctcaagcgc gaccgcggcg
     5701 agctgacgct catcgtggcg gccgccgact atgccgaggt ggcgcgcacc ctgcgtgacc
     5761 acgccgagct caagttcgag cagctgatcg acctgtgcgg cctggattac tcgagctaca
     5821 agaacgaggc ccacgagggc ctgcgcttct gcgtcgtgtc gcacctgctg tcggtgtcga
     5881 agaactggcg cttgcggctc aaggtgttct gccccgacga cgagctgccc atggtcgctt
     5941 cgctgaatga ggtctggagc tcggctaact ggttcgagcg cgaggctttc gacctctatg
     6001 gcatcgtctt cgagggccat gaggacctgc gccgcatcct gaccgactac ggcttcatcg
     6061 gccatccctt ccgcaaggac ttcccggtct ccggccatgt cgagatgcgc tacgaccccg
     6121 aacgcaagcg cgtgatctat cagccggtga cgatagagcc gcgcgagatc acgccgcgcg
     6181 tggtgcgcga agacaactat ggcgggctgc attgagctga gagcgacgca tggctgagat
     6241 caagaactac acgctcaatt tcggtcccca gcatccggct gcccacggtg tgctgcgcct
     6301 cgtgctcgag ctcgatggcg aggtgatcca gcgcgccgat ccgcacatcg gcctgctgca
     6361 ccgcgcgacc gagaagctgg ccgagacccg caccttcatc cagtcgctgc cctacatgga
     6421 ccgcctcgac tacgtgtcga tgatgtccaa cgagcacgcc tactgcctgg cgatcgagaa
     6481 gctgctgggc atcgaggtgc cgctgcgcgc gcagtacatc cgcgtgatgt tcgacgagat
     6541 cacgcgcttg ctgaaccacc tgctgtggct gggcgcgcat gggctggact gcggtgcgat
     6601 gaacatcctc atctactgct tccgcgagcg cgaagacctg ttcgacacgt acgaagcggt
     6661 gtcgggcgcg cgcatgcatg cggcctacta ccgtccgggc ggcgtctacc gcgatctgcc
     6721 cgactcgatg ccgcagtaca aggtctcgaa gatccgcaac gccaaggctc tggccaagat
     6781 gaacgagaac cggcagggct cgctgctgga cttcatcgac gacttctgcc ggcgcgtccc
     6841 gaagaacgtc gacgactacg agacgctgct gaccgacaac cgcatctgga agcagcgcac
     6901 cgtgggcatc ggcgtggtga gccccgagcg cgcgctgaac ctgggcttca ccggccccat
     6961 gctgcgcggc tccggcgtcg aatgggatct gcgcaagaag cagccctacg aggtctacga
     7021 ccgcatggac ttcgacgtgc cggtgggcag caacggcgac tgctacgacc gctacctggt
     7081 gcgcgtcgag gagatgcgcc agtccaaccg catcatcaag cagtgcgtgg actggctgcg
     7141 cgccaacccc ggcccggtca tcaccgacaa ccacaaggtg gcgccgcctt cgcgcctgga
     7201 catgaagtcc agcatggagg agctgatcca tcacttcaag ctcttcaccg aaggcttcca
     7261 cgtgcccgag ggcgaggcct atgcggcggt ggaacacccc aagggcgagt tcggcatcta
     7321 catcgtcagc gacggtgcca acaagcctta tcgcctcaag atccgtccgc cc
//
LOCUS       DASUCY010000051         2956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_16868, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000051 DASUCY010000000
VERSION     DASUCY010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16868"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..161
                     /locus_tag="VFV25_01065"
     CDS             <1..161
                     /locus_tag="VFV25_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HEY1391934.1"
                     /translation="AQEVWRRRFGGEPAEDAATRARQMRFLAGRGFSTEVIRRVVRGA
                     EKFGGAAD"
     gene            293..1813
                     /locus_tag="VFV25_01070"
     CDS             293..1813
                     /locus_tag="VFV25_01070"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY1391935.1"
                     /translation="MAAKKEQRSLFGEILDWMLAPLLLLWPMSVALTWLVAQNIANRP
                     YDRALGEMARALVQQVTIEETAGAAPKAKFKLPPGAADLLRADGVDDVYYQVLGTHGE
                     YLGGDRELPVPQDEAPVAGELRYRDDEMQAAPVRVAYLWGGAPPGGGSPPLVQVAETL
                     GKRSKLATEIIKGVILPQFVILPVAVLLVWLALARGISPLNELQQRIRQRAADDLSPI
                     DEHDAPEELSPLVLAVNDLLSRLDRSLRTQRRFLADAAHQLKTPLAGLRMQAELAQRD
                     IEAGKTDPKLMAASLQQIAHSSQRAAHMVNQLLAMARAEARAGDRKSWTQERISLPRL
                     AADTVRDFVPRALDKRIDLGYEGPEPLGPTVDNTSVMGQPLLLRELLSNLVDNALLYT
                     PAGGSATVRVVADPFGQVVVLQVEDSGPGIPASERELVFQPFYRALGTNVDGSGLGLA
                     IVAEIARQHGAELTLEDAHGPGSGEPPGARFTLRFPAAPPLQPAPQADADPNEVHP"
     gene            1817..>2956
                     /locus_tag="VFV25_01075"
     CDS             1817..>2956
                     /locus_tag="VFV25_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEY1391936.1"
                     /translation="MTTTAIARAVPKTQAPRPALFAIAWPIFAEFLLGMTVGGVGLWL
                     ASKTSDSAAGAFAMANHLMAALFILFRVVGIGVSVVVTQNLGAGRRAQADAVARAGLG
                     ASTWMGLGIALASLLLAGPLLRLMSTPPEVLPLAIPFLQTLAIALALDAYNATMASVM
                     RAHLHGRDALFVMGVMHAVHLLLAGPLMRGIGPLPGFGLVGFAMALAASRATGLALHL
                     WLWRLRLDLVPQRSDWWALPAQQLKGVLHIGLPGAAENIAYRLAFMVSVAVAAKMGAS
                     ALATQSYAMQVMYYALLFGLATGFASEIMVGHLIGAGALHEAHRLVRRNLMRGLAVST
                     SAALLAATCAPWLLRLFTHDPAIIEAATKLLWITVLLEPGRTFNLV"
BASE COUNT          404 a         1054 c         1065 g          433 t
ORIGIN      
        1 gcgctcagga ggtctggcgc cgccgcttcg gcggcgagcc ggccgaggac gcggcgacgc
       61 gcgccaggca gatgcgcttc ctggccgggc gcggcttctc gactgaggtg atacgccgcg
      121 tcgtgcgcgg cgccgagaag ttcggcggcg ccgccgacta gatgacggta cccggtcgcg
      181 ggttaccgcg accaccctcc gtgagggtgc cggcttgctt ctatgaggac cggcgcggcg
      241 ccggctaggg cctgttcgcg cggggcgcta tgctgcgcgt tcacaccgca gcatggccgc
      301 aaagaaggaa caacgctctc tcttcggcga gatcctcgac tggatgctcg cgccgctgct
      361 cttgctgtgg ccgatgagcg tggcgctgac ctggctggtg gcccagaaca tcgccaaccg
      421 cccttacgac cgtgcgctgg gcgagatggc ccgcgcgctg gtgcagcagg tgacgataga
      481 agaaaccgca ggcgccgcgc ccaaggcgaa gttcaagctg ccgcccggcg ccgccgacct
      541 gctgcgcgcc gacggcgtcg acgacgtcta ctaccaggtg ctgggcacgc acggcgaata
      601 cctgggcgga gaccgcgagc tgccggtgcc gcaggacgag gcgcccgtcg cgggcgagct
      661 gcgctaccgc gacgacgaga tgcaggccgc gccggtgcgc gtggcctatc tctggggcgg
      721 ggccccgccg ggcgggggct cgccgccgct ggtgcaggtg gccgagacgc tgggcaagcg
      781 cagcaagctc gccaccgaga tcatcaaggg cgtgatcctg ccgcagttcg tgatcctgcc
      841 ggtggcggtg ctgctggtgt ggctcgcgct ggcgcgcggc atctcgccgc tcaacgagct
      901 gcagcagcgc atacgccagc gcgcggccga cgacctgagc ccgatcgacg agcacgatgc
      961 gcccgaggaa ctgtcgccgc tggtgctggc cgtcaacgac ctgctgtcgc ggctggaccg
     1021 ctcgctgcgc acgcaaaggc gcttcctcgc cgacgcggcg caccagttga agacgccgct
     1081 ggccggcctg cgcatgcagg ccgagctggc ccagcgcgac atcgaggccg gcaagaccga
     1141 ccccaagctg atggccgctt cgctgcagca gatcgcccac agcagccagc gcgccgccca
     1201 catggtgaac cagctgctgg cgatggcgcg cgccgaggca cgcgccggcg atcggaaatc
     1261 ttggacccag gagcgcatca gcctgccgcg cctggcggcc gacacggtgc gcgacttcgt
     1321 gccgcgcgcg ctggacaagc gcatcgacct cggctacgag ggcccggagc cgctcggccc
     1381 gacggtcgac aacaccagcg tgatgggaca gcccctgctg ctgcgcgagc tgctgagcaa
     1441 cctggtcgac aacgccctgc tctacacgcc ggccggcggc tcggccacgg tgcgcgtggt
     1501 ggccgatccc ttcggccagg tggtggtcct gcaggtcgaa gactccggcc caggcatacc
     1561 ggcgagcgag cgggagctgg tgttccagcc cttctaccgg gccctgggca ccaatgtcga
     1621 cggctcgggc ctgggcctgg ccatcgtcgc cgagatcgca cgccagcatg gcgccgagct
     1681 cacgctcgag gacgcccacg gcccaggcag cggcgagccc ccgggggcgc gcttcacgct
     1741 gcgcttcccg gccgcgccgc ccttgcagcc ggcgccgcag gccgatgcgg acccgaacga
     1801 agtccatccc tgaaccatga ccacgactgc catcgcgcgc gccgttccca agacgcaggc
     1861 gcccaggccg gcgctgtttg ccatcgcctg gccgatcttc gccgagttcc tgctcggcat
     1921 gacggtgggc ggggtcgggc tgtggctggc ctccaagacc tccgacagcg cggccggcgc
     1981 cttcgcgatg gccaaccacc tgatggcggc gctgttcatc ctgttccgcg tggtcggcat
     2041 cggcgtcagc gtggtggtca cgcagaacct gggcgccgga cggcgcgcgc aagccgatgc
     2101 cgtggcgcgc gccggcctgg gggccagcac ctggatgggg ctgggcatcg cgcttgcctc
     2161 gctgctcttg gccgggccgc tgctgcgcct catgagcacc ccgccggagg tgctgccgct
     2221 ggccatcccc ttcctgcaaa cgctggcgat agcgctggca ctggacgcct acaacgccac
     2281 catggccagc gtgatgcgcg cccatttgca tggccgcgac gcgctgttcg tgatgggggt
     2341 catgcacgcg gtgcacctgc tgctggcggg cccgctgatg cgcggcatcg ggccgctgcc
     2401 cggcttcggg ctggtcggct tcgcgatggc gctggcggcc agccgcgcca ccggcctggc
     2461 gctgcatctg tggctgtgga gattgaggct cgatctggtg ccgcaacgca gcgactggtg
     2521 ggcgctgccc gcgcagcagc tgaagggcgt gctgcacatc ggcctgccgg gcgcggccga
     2581 gaacatcgcc tatcgcctcg ccttcatggt gagcgtggcg gtggcggcca agatgggcgc
     2641 gtccgcgctg gccacgcagt cgtatgcgat gcaggtgatg tactacgcgc tgctgttcgg
     2701 cctggccacc ggcttcgcca gcgagatcat ggtgggccat ttgataggcg ccggagcgct
     2761 gcacgaggcg caccggctgg tgcggcgcaa cctgatgcgc gggctggcgg tatccacctc
     2821 ggcggcactg ctggccgcca cctgcgcgcc ctggctgctg cgcctcttca cgcacgatcc
     2881 ggccatcatc gaggccgcca cgaagctgct gtggatcacc gtgctgctgg agccgggccg
     2941 caccttcaac ctcgtg
//
LOCUS       DASUCY010000052         2480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_354449, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000052 DASUCY010000000
VERSION     DASUCY010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354449"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="VFV25_01080"
     CDS             complement(<1..679)
                     /locus_tag="VFV25_01080"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY1391937.1"
                     /translation="MAQAHAGEQRFGKLTVRPAQRQLLVDGQPATVGARALDLLLALI
                     ERRERVVAKNELLDLVWPGLVVEENNLQVQISSLRKLLGPQVITTIPGRGYRFTATSP
                     GAAAAERPISDAVLGRRAGDTLPERNSNLGEPATLLGRDADLAALVPLVTAHRAVTVV
                     GAGGIGKTRLAQAAGHALRDVFTDGVWLVELAPLSDPQFVAAAAAQALGLSLPGHRPI
                     FDELVEAL"
     gene            848..1012
                     /locus_tag="VFV25_01085"
     CDS             848..1012
                     /locus_tag="VFV25_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1391938.1"
                     /translation="MSLSYTPVSKSSRLAAFALAVVFSTATLGAVVLGMTGASEIGFA
                     QSAPAAAVSV"
     gene            1032..1406
                     /locus_tag="VFV25_01090"
     CDS             1032..1406
                     /locus_tag="VFV25_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1391939.1"
                     /translation="MAAASAARSSHHQEFIMSYQRLIFVSALLVGSVGAHAQTGPVLE
                     STAKAMLASGPACGQAGAFSGAQRRLLQKASQGVGPLRDYVFITRAIHQYNMAEVGDW
                     IDGWRADQAHCGSTLAREEGKR"
     gene            1515..>2480
                     /locus_tag="VFV25_01095"
     CDS             1515..>2480
                     /locus_tag="VFV25_01095"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY1391940.1"
                     /translation="MDDISASPAYGFAGFELSPRERTVSLAGAPVKLGARAFDVLMAL
                     IERRERTVTKDELFDIVWPGLVVEESNLQVQVSALRKLLGQGVIATIPGRGYRFVAPL
                     DDRARAPWAPAVETPNNLPQQRTHFVGRAKELAQCSALLRDTRLLTLTGVGGCGKTRL
                     ALQLAQQQLDTFVDGVRFVDLASLQEAQGVAGALAGVIDVRERVGSPLVERMVEYLSL
                     RRELILLDNCEHVIDAVADLCEALLARCPQLKIVATSREGLGLDGEQLYSVQPLSLPT
                     TPELQAMDASEAVRLLVDRARLAVPDLVLDERNAAVVADICCRLDG"
BASE COUNT          373 a          848 c          853 g          406 t
ORIGIN      
        1 gcagcgcctc gaccagctcg tcgaagatcg gtcggtgtcc cggcaggctc agccccagcg
       61 cctgcgcggc tgcggcggcg acgaactgcg ggtcggacag cggcgccagt tcgaccagcc
      121 agacgccatc ggtgaacacg tctcgcaggg catgcccggc agcctgggca aggcgggtct
      181 tgccgatgcc gccggcgccc acgacggtga cggctcggtg agccgtcacc aatggcacca
      241 gcgccgcaag gtcggcgtcg cggccgagta gtgtcgccgg ctcgccgaga ttgctgttgc
      301 gctcgggcag cgtgtcgccg gcgcggcgcc caaggactgc gtctgaaatg ggcctctcgg
      361 ctgccgccgc accaggggat gtggcggtaa atcggtaacc ccggccgggg atggtggtaa
      421 tcacctgcgg gccgagcagc ttgcgcaagc tgctgatctg cacctgcagg ttgttctcct
      481 cgaccaccag gcccggccag accaggtcga gcagctcgtt cttggccacg acgcgctcgc
      541 ggcgctcgat cagcgccagc agcaggtcca gcgcgcgtgc gccgacggtc gctggctggc
      601 cgtcgaccag cagctggcgc tgggccggcc tgaccgtcag cttgccgaaa cgctgctccc
      661 ctgcatgggc ctgcgccatg ggcacctccg cacctcccaa tttagcggga attggcgcgt
      721 ccggggggca ccctgagggg gggcgattca gaaggtttaa ggacgcttca gcggcgcttc
      781 aagacttcat aggccccgtc gcccaaactg attccatcga gcaaccaacc caccatggag
      841 tcagatcatg agcctcagct acaccccggt ttccaagtcc tcccggctgg ccgcctttgc
      901 cctggccgtc gtcttcagca ccgccacgct cggcgccgtg gtgctgggca tgaccggcgc
      961 gtcggagatc ggctttgcgc aatccgcgcc cgccgcggcc gtatccgtgt gaagcggaca
     1021 gacatccccc catggccgcc gcgagcgcgg cccgttcttc tcaccaccag gagttcatca
     1081 tgagctatca acgcctgatt ttcgtctcag ccctgctggt cggttcagtc ggtgcccacg
     1141 cgcagaccgg tcccgtgctg gaatccaccg cgaaagccat gctggctagc gggcccgcct
     1201 gcggacaagc cggtgctttc agcggagcgc agcggcggct gctgcagaag gcctcgcaag
     1261 gggtggggcc gttgcgcgac tacgtcttca tcacgcgcgc catccatcag tacaacatgg
     1321 ccgaagtcgg cgactggatc gacggctggc gcgccgacca ggcccactgc ggctcgacgc
     1381 tggcgcgcga agaaggcaag cgctgagcat cgggcggcct cgcgccgccc gtgctacccg
     1441 attgcggcgg ttgctaccca cgtgctcagc gactaacctc attgaaacct tcgatgcatc
     1501 gcacgggggc tcgcatggac gacatatcgg cttccccggc ctacggcttt gccggatttg
     1561 agctcagccc ccgcgagcgg acggtgtcgc ttgccggtgc gccggtcaag ctcggtgccc
     1621 gggccttcga cgtgctgatg gccctcatcg agcgtcgcga gcgcacggtg accaaggacg
     1681 agctgttcga catcgtgtgg cccggcctcg tggtcgagga gagcaatctg caggtgcagg
     1741 tgagcgccct gcgcaagctg ctcgggcaag gcgtgatcgc gaccatcccg gggcgcggct
     1801 atcgcttcgt cgcgccgctg gacgatcgcg cccgcgcgcc ctgggcgccg gcggtggaga
     1861 cgccgaacaa cctgccgcag cagcgcacgc acttcgttgg ccgggcgaag gagctggcgc
     1921 aatgcagcgc gctgctgcgc gacacccgac tgctgacgct gacgggcgtg ggcgggtgcg
     1981 gcaagacgcg cctggccctg cagttggcgc agcagcagct ggacacgttt gtcgacggcg
     2041 ttcgattcgt cgatttggcc tcgctgcagg aggcgcaagg cgtagcgggc gcccttgccg
     2101 gcgtcatcga tgtgcgtgag cgcgtcggtt cgccgctggt cgagcgaatg gtcgaatacc
     2161 tgtcgctacg ccgagaactg atcctgctgg acaactgcga gcatgtgatc gacgccgtgg
     2221 ccgatctctg cgaggccctg ctggcccgat gcccccagct gaagatcgtg gctaccagcc
     2281 gcgaggggct gggcctcgat ggcgagcagc tgtactccgt gcagccgctg tccttgccga
     2341 cgacgcccga gcttcaggcg atggacgctt ccgaagcggt gcgcctgctg gtcgatcggg
     2401 cgcggctggc cgtgcccgat ctggtgctgg acgagcgcaa tgcagccgtg gtggccgaca
     2461 tctgctgccg gctcgacggc
//
LOCUS       DASUCY010000053         3577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_788311, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000053 DASUCY010000000
VERSION     DASUCY010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788311"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VFV25_01100"
     CDS             complement(<1..183)
                     /locus_tag="VFV25_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY1391941.1"
                     /translation="MGAPVTVEGLTIVAGEQTLVDQLSFAIRPGEVLALIGESGSGKT
                     TTALALMGYARHGCRIA"
     gene            complement(183..1070)
                     /locus_tag="VFV25_01105"
     CDS             complement(183..1070)
                     /locus_tag="VFV25_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013210078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY1391942.1"
                     /translation="MDAISPSGAALQATARKRTARHRLLGFGWTGLLGLAVLAFWLLA
                     AVFGPSLARYDMSSISSGAAVFAPMSVQHWLGTDYLGRDMLARVLDGARYTVGVALVA
                     TLLASGIGTTLALLAATTGRWIDATLSRGLDTLTAIPSKMFALIIVAGFGSSTAMLIA
                     TAAIIYVPGAYRIARSLAININAMDYVVVARTRGEGTLYIMLREILPNIVGPMLADLG
                     LRFVYVVLLLAGLSFLGLGVQPPAADWGSLVRENIGALPEGGAAVIAPAMAIASLTIA
                     VNLVIDNLPGRAARERGAR"
     gene            complement(1079..2035)
                     /locus_tag="VFV25_01110"
     CDS             complement(1079..2035)
                     /locus_tag="VFV25_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003265957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY1391943.1"
                     /translation="MNVLILRLLAQRIALALLSLLAVSVIVFAITAVLPGDAAQEQLG
                     QEATPEALAALRAQMGLDVPGPVRYWRWLSGMLQGNPGMSSVTSMPVGELIASRLPNS
                     LLLAAVTALFSVPIALTLGIASAVWRGSWFDRIASTAAVGVVSVPEFLVATLAVLVFA
                     VNLHWLPALSFVNDVESVGQMLRAFAMPVLSLCCVIVAQMMRMSRAAVIDQLEAPYIE
                     MVKLKGASPLRMVLAHALPNAVGPIANAVALSLSYLLGGVIIIETIFNYPGIAKLMVD
                     GVTQRDMPLVQTCAMIFCAGYLILVTLADLCGIVANPKLRHR"
     gene            complement(2061..3575)
                     /locus_tag="VFV25_01115"
     CDS             complement(2061..3575)
                     /locus_tag="VFV25_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY1391944.1"
                     /translation="MLMAGGMQVGLAGSLAGVAVSAHAQTPKKGGRLRVAGATAAVTD
                     TLDPAKQSNQTDYSRGTMLYNGLTSLDGSLTPQPALAESFATKDAKTWVFTLRKGVSF
                     HDGKPLSPADVVFSLMRHKDPATGSKAKALADQIDSVKATGPNEVTIQLSAANADLPV
                     FLGTFHFHIVKDGTTDFNAGIGTGPYKLKEFKPGVRSLMVRNERYWKPGKPYLDEIEF
                     VGIGDETARVNALLSGGMDLVASVNPRAVARVQATPGYAIFTTQSGQYSDLILRKDLG
                     PGANPDFVLAMKYLFDREQMKKSIALDHAVVANDQPIDPTNRFYFAGLPQRAFDLDKA
                     KFHLQKSGVTGTVPVVTSPAAQYSVEIALLLQQAAQKIGLDLEVKRMPADGYWSNHWL
                     NSPVGFGNINPRPSADTLLTQFFKSDAAWNESRWKNPKFDQLLAASRSETDLAKRKQM
                     YADMQTLINQEAGLGIPLFLASIDGHSTKVKGLSPIPLGGLMGYSFAEHVWIES"
BASE COUNT          604 a         1167 c         1248 g          558 t
ORIGIN      
        1 cgcgatgcga cagccgtggc gcgcatagcc catcagtgca agcgccgtgg tggtcttgcc
       61 cgagcccgac tcgccgatca gcgccagcac ctcgccgggg cggatagcga agctcagctg
      121 atcgacgagc gtctgctcgc cggcgacgat ggtgagtcct tcgacggtga ctggcgcgcc
      181 catcagcgtg ctcctcgctc gcgagcggcg cggcccggca ggttgtcgat cacgaggttg
      241 accgcgatcg tgaggctggc gatcgccatc gccggcgcga tcaccgcggc gccgccctcg
      301 ggtagcgctc cgatgttctc gcgcaccagc gagccccagt cggcggccgg cggctgcacg
      361 cccaggccga ggaagctcag gcccgcgagc aacagcacga cgtagacgaa gcgcagcccc
      421 aggtcggcca gcatcgggcc gacgatattg ggcaggatct cgcgcagcat gatgtagagc
      481 gtgccttcgc cgcgcgtgcg cgcgacgacc acatagtcca tcgcattgat gttgatcgcc
      541 agcgagcgcg cgatgcggta ggcgcccggc acgtagatga tggcggcggt ggcgatcagc
      601 atcgcggtcg acgagccgaa gccggccacg atgatcagcg cgaacatctt gctcggaatc
      661 gccgtcagcg tgtccagccc gcggctgagc gtggcgtcga tccagcgccc agtggtggca
      721 gccagcagcg ccaacgtggt gccgatgccg ctggccagca gcgtggcgac cagcgcgacg
      781 ccgaccgtgt agcgcgcacc atccagcacg cgcgccagca tgtcgcggcc gaggtagtcg
      841 gtgcccagcc agtgctgcac gctcatcggc gcgaacaccg ctgcgccgct gctgatgctg
      901 ctcatgtcgt agcgggccag ggaggggccg aagacggctg ccagcagcca gaaggccagc
      961 accgccaggc ccagcaggcc ggtccagccg aagccgagca gccgatgacg tgccgtgcgc
     1021 ttgcgagccg tggcctgcaa ggctgcgccg gaaggggaga tggcatccat gggggtcttc
     1081 agcggtggcg cagcttggga ttggcgacga tcccgcacaa gtcggccagg gtcaccagga
     1141 tcaggtagcc ggcgcagaag atcatggcgc aggtctgcac cagcggcatg tcgcgctggg
     1201 tgacgccgtc gaccatcagc ttggcgatgc cggggtagtt gaagatggtc tcgatgatga
     1261 tcacgccgcc cagcagatag gacaggctca gggccaccgc attggcgatc ggccccacgg
     1321 cattgggcag cgcgtgcgcg agcaccatgc gcagcggcga agcgcccttg agcttgacca
     1381 tctcgatgta gggcgcctcc agctggtcga tcacggctgc gcggctcatg cgcatcatct
     1441 gcgcgacgat cacgcagcac aggctgagca caggcatcgc gaaagcgcgc agcatctgcc
     1501 cgaccgactc gacgtcattg acgaaggaca gcgccggcag ccagtgcaga ttgaccgcga
     1561 acaccagcac cgcgagcgtg gcgaccagga actcgggcac cgacaccacg ccgaccgcgg
     1621 cggtcgacgc gatgcggtcg aaccatgagc cgcgccatac cgccgacgcg atgccgagcg
     1681 tgagcgcgat gggcaccgag aacagcgccg tgacggccgc cagcagcagc gagttgggca
     1741 gccggctggc gatcagctcg cccactggca tggacgtgac cgacgacatg cccgggttgc
     1801 cctgcagcat gccggacagc cagcgccagt agcgcaccgg tccgggaacg tccaggccca
     1861 tctgcgcgcg cagcgcggcc agggcctcag gcgtggcctc ctggccgagc tgctcctgcg
     1921 ccgcgtcgcc gggcagcacg gcagtgatcg cgaacacgat caccgacacc gccagcagcg
     1981 acagcagcgc cagcgcaatc cgctgggcaa gaagtctgag gatcaggacg ttcatcggat
     2041 ggctggcgcc tcggcgagtg tcaggactcg atccagacgt gctcggcgaa agagtagccc
     2101 atcaggccgc cgagcgggat gggcgacaag cccttgacct tggtgctgtg gccgtcgatg
     2161 ctcgcgagga agagcggaat accgaggccg gcttcctggt tgatcagcgt ctgcatgtcg
     2221 gcgtacatct gcttgcgctt ggccagatcc gtctccgagc gcgaggccgc cagcagctga
     2281 tcgaatttcg ggttcttcca gcgcgactcg ttccacgcgg cgtcggactt gaagaactgc
     2341 gttagcagcg tgtcggcgct cgggcgcggg ttgatgttgc cgaagccgac cgggctgttg
     2401 agccagtggt tcgaccagta gccgtcagcc ggcatgcgct tgacctccag atccagcccg
     2461 attttctgcg ccgcctgctg cagcaacagc gcgatttcga cagagtactg cgcagccggg
     2521 gaggtcacca ccggcaccgt gccggtgacg cctgattttt ggaggtggaa cttggccttg
     2581 tcgaggtcga aggcccgctg cggcaggccg gcgaagtaga agcggttcgt ggggtcgatc
     2641 ggctggtcgt tggcgaccac cgcatggtcc agcgcgatgg acttcttcat ctgctcgcga
     2701 tcgaagaggt atttcatcgc cagcacgaag tccgggttgg cgccggggcc caggtctttg
     2761 cgcaggatca ggtcggagta ctggcccgac tgcgtggtga agatcgcata gccgggggtt
     2821 gcctgcacgc gggcgacggc gcgcggattc accgaggcga ccaggtccat gccgcccgac
     2881 agcagcgcgt tgacgcgcgc cgtctcgtcg ccgatgccga cgaattcgat ctcgtcgagg
     2941 tagggcttgc cgggcttcca gtagcgctcg ttgcgcacca tcagcgagcg cacgccgggc
     3001 ttgaattcct tcagcttgta ggggccggtg ccgatgcccg cattgaagtc cgtcgtgccg
     3061 tccttgacga tatggaagtg gaaagtgccc aggaacaccg gcaggtcggc gttggctgcg
     3121 gacagctgta tcgtcacctc gttggggccg gtggccttga cgctgtcgat ctggtctgcc
     3181 agggccttgg ccttggagcc ggtcgcagga tccttgtggc gcatcagcga gaacaccaca
     3241 tcggccggcg atagcggctt gccgtcgtgg aagctcacgc ccttgcgcaa ggtgaacacc
     3301 caggtctttg cgtccttggt cgcgaacgac tcggccagcg cgggctgcgg cgtgaggctg
     3361 ccgtccagcg aggtcagccc gttgtagagc atggtgccgc gcgaatagtc ggtctgattc
     3421 gactgcttgg cggggtccag cgtgtcggtg acggcggctg tggcgcccgc cacgcgcaga
     3481 cggccgccct tcttcggtgt ctgcgcatgc gcggacactg caaccccagc caggcttccg
     3541 gccaggccca cttgcatgcc gccggccatc aacatcg
//
LOCUS       DASUCY010000054         4455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_386605, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000054 DASUCY010000000
VERSION     DASUCY010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386605"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VFV25_01120"
     CDS             <1..342
                     /locus_tag="VFV25_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003081116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEY1391945.1"
                     /translation="IVFVNANPQSTLLLGQARGAALAGLVSCELAVSEYTALQMKKAV
                     AGHGLARKEQIQEMVARLLRLPGAPGKDAADALGIAICHAHAGRSMAALAQATPLARK
                     AHAQYRKGRTY"
     gene            complement(390..590)
                     /locus_tag="VFV25_01125"
     CDS             complement(390..590)
                     /locus_tag="VFV25_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1391946.1"
                     /translation="MLTWIATIAIGSTLAITAFCFVAGLTVDDERWQARTVYWFRWTV
                     VATLGSFNIAIFRHIIEALLSF"
     gene            complement(611..796)
                     /locus_tag="VFV25_01130"
     CDS             complement(611..796)
                     /locus_tag="VFV25_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1391947.1"
                     /translation="MPLARTLVLLLLLAGVACFALYAATGQQLWRRRGLMIVRWTVYA
                     ALSFFAVLMLQRLAHVV"
     gene            complement(813..1748)
                     /gene="lpxC"
                     /locus_tag="VFV25_01135"
     CDS             complement(813..1748)
                     /gene="lpxC"
                     /locus_tag="VFV25_01135"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEY1391948.1"
                     /translation="MLQQRTLKSLTRAVGVGVHSGQRVELVLRPAPANTGIVFRRVDL
                     PEPVDIPVSPDAVCDTRMASTISPGGDPGAPKVQTIEHLMSACCGLGLDNLYVDINAE
                     EVPILDGSAASFVFLLQSAGIELQRAPKRFLKVLKEIEIREGSGPTLKWARLAPYNGY
                     KLSFEIDFAHPAVDQTGQRVTFDFSSGQYKRDIARARTFGFTKDVEMARARGLALGGS
                     MDNAIVVDDYRVLNAEGLRYDDEFVKHKILDAIGDLYLACKPLLASYTSYKGGHALNN
                     KLLRALLADDYAYEIVSFENEAQAPQGFAELAPAW"
     gene            complement(1858..3054)
                     /gene="ftsZ"
                     /locus_tag="VFV25_01140"
     CDS             complement(1858..3054)
                     /gene="ftsZ"
                     /locus_tag="VFV25_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEY1391949.1"
                     /translation="MAIEMIEEFNLGTQIKVIGVGGGGGNAVEHMIAEDVQGVEFICA
                     NTDAQALNRSSAHNLIQLGTTGLGAGSKPEAGRAAAEEAVDRIRAAIDGAHMIFLTAG
                     MGGGTGTGAAPVIARVAKEMGILTVGVVTKPFDFEGGRRMKQAESGLQELEANVDSLI
                     VVLNEKLLEVLGDDVSQDQAFKHANDVLKNAVGGIADIIHIDASINVDFEDVKTVMCE
                     PGKAMMGTAMAAGPDRAQRAADAAVACPLLEGIDLSGARGVLVLIAASKATLKLKESQ
                     LAMNTIRRYAAEDAHVIFGAAYDDSLGDQLRVTVIATGLAPAKRVQTPVTMVHNAVAQ
                     RTGTDNIPVLTTPVSASGGPDYTGLAVPSVWRSGRSQAAAKVDALASNGMDEIEIPAF
                     LRKQAD"
     gene            complement(3116..4345)
                     /gene="ftsA"
                     /locus_tag="VFV25_01145"
     CDS             complement(3116..4345)
                     /gene="ftsA"
                     /locus_tag="VFV25_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEY1391950.1"
                     /translation="MAKEYKDLVVGLDIGTAKVMAVVAEVLPGGELRVAGLGVAATHG
                     LKRGVVVNIDATVQSIQQALKEAEMMADCKISRVYTGITGSHIRGQNSTGMVIVRDKE
                     VTPTDVARVVETAKAINIPNDQRLLLVEPQEFVIDGHEVKEPIGMSGGRLEVKVHIVT
                     GAQSAAENIVKCVRRCGLEVEQLVLNPSASSLAVLTDDERDLGVAVVDIGAGTTDVSI
                     FTDGSVRHTAVIPIAGDLITSDIAMALRTPTKDAEEIKVDYGVAKQLLADPGEQVEVP
                     GLGDRAPRMLSRQALAGVIEPRVEEIFSLVHQVIRESGYEELLSSGIVLTGGAAVMPG
                     MVELGEDIFLKPVRKGLPTYSGALFDMVASPRSATVMGLLEEARLARTRGIKAAQQAG
                     SVQTLFGRVKDWFIGNF"
BASE COUNT          687 a         1493 c         1504 g          771 t
ORIGIN      
        1 atcgtcttcg tcaatgccaa tccccagtcc acgctgctgc tgggccaggc ccgcggcgcg
       61 gcgctggcgg ggctggtgag ctgcgagctg gcggtgagcg aatacaccgc gctgcagatg
      121 aaaaaggccg tcgcaggcca cggcctggcg cgcaaggagc agatccagga gatggtggcg
      181 cgcctgctca ggctgccggg cgcacccggc aaggatgcgg ccgatgcgct gggcattgcg
      241 atctgccatg cccatgcggg ccgctcgatg gccgcgctgg cgcaggccac gccgctggcg
      301 cgcaaggccc atgcgcagta ccgcaagggc aggacttact gagcactgaa agcgctccag
      361 cccccgtggg gtcgaggctc agaatgcgcc tagaagctca gcagcgcctc gatgatgtgc
      421 ctgaagatgg cgatgttgaa gctgcccagc gttgccacca cggtccagcg gaaccagtag
      481 acggtgcgcg cctgccagcg ctcgtcgtcg accgttaggc cggccacgaa gcagaacgcg
      541 gtgatggcga gtgtcgaacc tatggcaatg gtggcgatcc aggtcagcat ctcaggtcgg
      601 caagtccgtt tcagacgaca tgggccaggc gctgcagcat cagcactgcg aagaagctca
      661 gcgccgcata gacggtccat ctcacgatca tcagtccgcg acggcgccag agctgctggc
      721 cggttgcggc gtacagcgcg aagcatgcca cgcctgccag cagcaacagc aggacgaggg
      781 tgcgggcgag cggcatgcga gcttaagctc gatcaccagg ccggggccag ttcggcaaag
      841 ccctgaggcg cctgcgcttc gttctcgaag ctgacgatct cgtaagcata gtcatcggcc
      901 agcaaggcgc gcagcagctt gttgttcagc gcgtggccgc ccttgtagga ggtgtagctc
      961 gccagcagcg gcttgcaggc gaggtagagg tcgccgatcg catccaggat cttgtgcttg
     1021 acgaactcgt cgtcgtagcg caggccctcg gcattgagca cgcggtagtc gtcgaccacg
     1081 atggcgttgt ccatgctgcc gcccagggcc agcccgcggg cgcgcgccat ttccacgtcc
     1141 ttcgtgaagc cgaaggtgcg cgcacgcgcg atgtcgcgct tgtactggcc ggagctgaag
     1201 tcgaaggtca cgcgttggcc ggtctggtcg accgcgggat gagcgaaatc gatctcgaag
     1261 ctgagcttgt agccgttgta gggcgcaagc ctggcccatt tgagcgtggg gccgctgcct
     1321 tcgcggatct cgatctcctt cagcaccttg aggaagcgct tcggggcgcg ctgcagctcg
     1381 atgccggcgc tttgcagcag gaagacgaag gaggcggcgg aaccgtcgag gatgggcact
     1441 tcctcggcgt tgatgtcgac gtagaggttg tccagtccga ggccgcagca ggcagacatc
     1501 aggtgctcga tggtctgcac cttgggtgcg ccgggatcgc cgcccggcga gatcgtcgag
     1561 gccatgcgcg tgtcgcacac ggcgtcgggg ctgaccggga tgtccaccgg ctcgggcagg
     1621 tcgacgcggc ggaacacgat accggtgttg gccggggcgg ggcgcagcac gagttcgacg
     1681 cgctgcccgc tgtgcacgcc cacgccgacg gcgcgggtca gggacttgag ggtgcgctgc
     1741 tgcaacatca tgccttcatt gtaagaacgc gggccggagc gtgtgctccg aagggcaaag
     1801 cagccccgat gccggtccag acatcggggc cttgcgacga aggagagaaa gccgagctca
     1861 gtctgcctgc ttgcgcagga aagccgggat ctcgatctcg tccatgccgt tgctcgccag
     1921 tgcatccacc ttggccgccg cctggctgcg gccgctgcgc cacacgctgg gcacggccag
     1981 gcccgtgtag tcggggccgc cggacgcgct caccggagtg gtcagcaccg ggatgttgtc
     2041 ggtgccggtg cgctgggcga ccgcgttgtg caccatcgtc accggcgtct gcacgcgctt
     2101 ggccggcgca aggccggtgg cgatcaccgt gacgcgcagc tggtcgccca ggctgtcgtc
     2161 ataggcggcg ccgaagatca cgtgggcgtc ctcggccgca tagcggcgga tggtgttcat
     2221 cgcgagctgg ctttccttga gcttcagcgt ggccttgctg gccgcgatca gcaccagcac
     2281 gccgcgcgcg ccggacaggt cgatgccttc gagcagcggg caggccaccg ccgcgtcggc
     2341 ggcgcgctgg gcgcggtcgg ggccggccgc catcgccgtg cccatcatcg ccttgcccgg
     2401 ctcgcacatc accgtcttca cgtcctcgaa gtcgacgttg atcgaggcgt cgatatggat
     2461 gatgtcggcg atgccgccca cggcgttctt cagcacgtcg ttggcgtgct tgaaggcctg
     2521 gtcctgggag acatcgtcgc ccagcacctc gagcagcttc tcgttcagca ccacgatcag
     2581 cgaatcgaca ttcgcctcga gctcctgcag gccggactcg gcctgcttca tgcggcgccc
     2641 gccctcgaag tcgaagggct tggtcaccac gcccaccgtc aggatgccca tctccttggc
     2701 tacgcgggcg atcaccgggg cggcgccggt gccggtgccg ccgcccatgc cggcggtcag
     2761 gaagatcatg tgggccccgt cgatggcggc gcggatgcga tccaccgcct cttcggccgc
     2821 ggcgcggccc gcctcgggct tggaacccgc gcccagcccg gtggtgccga gctggatcag
     2881 gttgtgggcc gacgagcggt tgagcgcctg ggcgtcggtg ttggcgcaga tgaactccac
     2941 gccctgcacg tcctcggcga tcatgtgttc gacggcgttg ccgccgccgc ctccgacgcc
     3001 gatcaccttg atctgcgtcc ccaggttgaa ctcttcgatc atttcgatgg ccatgagtgt
     3061 tactccttcg tcgtcgtcaa gcagttgcct aaaaagtccc aaatgaaacg ccggttcaaa
     3121 agttgccgat gaaccagtcc ttgacgcggc cgaacagggt ctggaccgac cccgcctgct
     3181 gcgccgcttt gatcccgcgc gtacgcgcga gccttgcttc ttcgagcaat cccatcaccg
     3241 tcgccgagcg cgggctcgcc accatgtcga acagagcgcc cgaataggtg gggaggccct
     3301 tgcgcacggg cttcaggaaa atgtcttcgc cgagctcaac catgcccggc atcaccgccg
     3361 cgccgccggt cagcacgatg ccgctggaca gcagctcctc gtagccgctc tcgcgtatca
     3421 cctggtgcac gagcgagaag atctcctcga cgcgcggctc gatcacgccg gccagcgcct
     3481 ggcggctgag catgcgcggc gcgcggtcgc ccagcccggg cacctcgacc tgctcgcccg
     3541 ggtcggccag cagctgcttg gccacgccgt agtcgacctt gatctcttcc gcgtccttgg
     3601 tcggcgtgcg cagcgccatc gcgatgtcgc tggtgatcaa gtcgccggcg atcgggatca
     3661 cggccgtgtg gcgcaccgag ccgtcggtga agatcgacac gtcggtggtg ccggcgccga
     3721 tgtcgaccac agccacgccg aggtcgcgct cgtcgtcagt cagcacggcc aggctggacg
     3781 cgctggggtt gagcaccagc tgctccacct ccagcccgca gcggcgcacg cacttgacga
     3841 tgttctcggc cgcgctctgc gcgccggtca cgatgtgcac cttcacctcc aggcgcccgc
     3901 cgctcatgcc gatcggctcc ttgacctcgt ggccgtcgat cacgaactcc tggggctcga
     3961 ccagcagcag acgctggtcg ttggggatgt tgatggcctt ggccgtctcg accacgcgcg
     4021 ccacgtcggt gggcgtgacc tccttgtcgc gcacgatcac catgccggtg gagttctggc
     4081 cgcggatatg gctgccggtg atgccggtgt agacgcggct gatcttgcag tcggccatca
     4141 tctcggcttc tttcagcgcc tgctggatcg actgcacggt ggcatcgatg ttgaccacga
     4201 cgccccgttt gagcccgtgc gtcgccgcca cgccgagccc cgccacccgc agctcgccgc
     4261 cgggcagcac ttcggccacc accgccatca ccttggcggt gccgatgtcc agtccgacga
     4321 cgagatcctt gtattccttg gccatttcgt cctcaacgtt tctgttttcc gggtttaccc
     4381 tgagtcgtca cgccgcgcag cttcagcgcg tagccgtcgg catggcgcag atcggcgctg
     4441 agcagcgggc tgtgg
//
LOCUS       DASUCY010000055         4865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_113626, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000055 DASUCY010000000
VERSION     DASUCY010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4865
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113626"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1464
                     /locus_tag="VFV25_01150"
     CDS             <1..1464
                     /locus_tag="VFV25_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY1391951.1"
                     /translation="GPPGDRGPSSLDWALQEGATQWGVTVLEAVAEMDAGDIWAWAQV
                     QVSPVGKSDLYRGEVADAAADAVLLAVERFASGHHRPQRQDPAALAAGWRPFIRQPQR
                     RIDWATQDTASVLRQLRAADSQPGVLDELLGEPWFLHGGYPEDELRGRPGELIATRDG
                     AICRATVDGAVWIPQLRSQRRPDGPSTFKLPAVDALGSRLPALPEMPAPLELPPGRRT
                     WSEIRYREQGRVGFLQFGFPSGAMSTRQCKRLLEAYRYACSRDTEVLVLGGLRDFFSN
                     GIHLNVIEAAADPAQESWANINAMNDLVQAVLTTTDRLTVCALGGNAAAGGVMLALAA
                     DEVWCRDGLVLNPHYRLMGLYGSEYWTYQLPRRVGAERAQQLVETALPVSTAAALRMG
                     LIERVLQASPEEFGDEVARLAAQLAKSPKRRDRLARKCAERERDEALKPLQQYRDEEL
                     AHMHRNFWGDNEPYHALRSAFVHKLLPVRTPDYLCGE"
     gene            1787..2569
                     /locus_tag="VFV25_01155"
     CDS             1787..2569
                     /locus_tag="VFV25_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHYT domain-containing protein"
                     /protein_id="HEY1391952.1"
                     /translation="MDINVGDVLQARYDMVAVATSYVVSVLGSYAALAHTPHMFNRDG
                     SLKWSMVVGAAVALGGIGIWTMHFIGMIGYHLPFRVVYEGTLTLVSLAAAIVIAGIAL
                     VLPGLGGRFSLGGWAVGSVLAGLGVCVMHYMGMFAMNLQAAMNLDMKIVGLSVAIAIT
                     AAAAALWLAYHVRKTLHRAVAAVVMGLAVCAMHYTGMSAAEFICVAQTPASVWGVGGS
                     DLLPVVFGVASVVLAVLTWNVLGIFVEDGPSPVMAKPRASAF"
     gene            complement(2743..2818)
                     /locus_tag="VFV25_01160"
     tRNA            complement(2743..2818)
                     /locus_tag="VFV25_01160"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2783..2785),aa:Ala,seq:cgc)
     gene            complement(2840..3712)
                     /locus_tag="VFV25_01165"
     CDS             complement(2840..3712)
                     /locus_tag="VFV25_01165"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY1391953.1"
                     /translation="MKKIAWLGTGLMGAPMARRLCEAGFELTAWNRTPAKAQALAQHG
                     ARVGGSPAQAVLGADIVVTMLEHGGVVRDVLFEQGAATALQRGALVIDMSSIKPSEAR
                     EHAAALAALGMAHLDAPVSGGTLGAEAGTLAIMVGGEPADFERARPLFQHLGRATHVG
                     PHGAGQLAKLANQMIVGIGIGAVAEALLFAAKGGADMAGVREAIRGGFAESRVLEVHG
                     LRMVERDFAKRGTLAVQLKDLRNALQTAAELGFSAPVTQLLESLYAEAAADGLASQDQ
                     SALFVELARRNGLA"
     gene            complement(3709..4395)
                     /gene="rsxB"
                     /locus_tag="VFV25_01170"
                     /pseudo
     CDS             complement(3709..4395)
                     /gene="rsxB"
                     /locus_tag="VFV25_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577060.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
     gene            complement(4447..>4865)
                     /locus_tag="VFV25_01175"
     CDS             complement(4447..>4865)
                     /locus_tag="VFV25_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HEY1391954.1"
                     /translation="YDFYEDLLRGDLEDAEAHREFYDEYNAVLDMPAEYYLDTIKTVF
                     QDFALVNGSWKVGGQPVRPQDIKGTALLTVEGELDDISGAGQTEAAHSLCTGIAKTRQ
                     RHYSVKGAGHYGIFSGRRWREKAYPEIRDFIAQYDK"
BASE COUNT          764 a         1650 c         1618 g          833 t
ORIGIN      
        1 ggcccgccgg gcgaccgcgg gccctcgtcg ctggactggg cattgcagga aggcgccacg
       61 cagtggggtg tgaccgtact ggaggccgtg gccgaaatgg atgccggcga catctgggcc
      121 tgggcgcaag tgcaggtttc tccggtcggc aagagcgacc tgtatcgcgg cgaggtggcc
      181 gatgccgccg ccgacgcagt gctgctggcg gtggagcgct ttgcgtccgg ccaccaccgg
      241 ccccagcggc aggacccggc cgcgctggcc gccggctggc gccccttcat ccgccagccg
      301 cagcgtcgca tcgactgggc gacgcaggac acggcctcgg tgctgcggca gctgcgcgcg
      361 gccgactcgc agcccggcgt gttggacgag ttgctgggcg agccctggtt cctgcacggc
      421 ggctaccccg aagacgagct gcgcggccga ccgggcgaac tgatcgccac ccgcgacggt
      481 gccatctgcc gtgccaccgt ggacggcgcc gtctggattc cgcagctgcg gtcgcagcgc
      541 cggcccgacg ggccgagcac attcaagctg cccgcggtcg acgccctcgg ctcacggctg
      601 ccagcgctgc cggaaatgcc tgcgccgctc gagctgccgc ccggacgccg cacctggtcc
      661 gagatccgct atcgcgagca aggtcgggtg ggcttcctgc aattcggctt cccgagcggc
      721 gccatgagca cgcggcagtg caagcgcctg ctggaggcct atcgctacgc ctgcagtcgc
      781 gacaccgagg tgctggtgct gggtggcctg cgtgatttct tctccaacgg catccacctc
      841 aacgtgatcg aggccgcggc cgacccggcg caggaatcct gggccaacat caatgccatg
      901 aacgacctgg tgcaggcggt gctgaccacc accgacaggc tcacggtctg cgcgctgggc
      961 ggcaacgcgg ccgccggcgg cgtgatgctg gcattggcgg ccgacgaagt gtggtgccgc
     1021 gacggcctgg tgctgaaccc gcattaccgg ctcatgggcc tgtacggctc cgaatactgg
     1081 acctaccagc tgccgcgccg cgtcggcgcc gagcgcgcac agcaactcgt ggagactgca
     1141 ctgccggtga gcaccgccgc cgccttgcgc atgggcctga tcgagcgcgt gctgcaggct
     1201 tcaccggaag agttcggcga cgaggtggcg cggctggccg cgcagttggc caaatcaccc
     1261 aagcgccgcg accgcctcgc ccgcaagtgc gccgagcgcg agcgcgacga agcactcaag
     1321 ccgctgcaac agtaccgcga cgaggaactc gcgcacatgc accggaattt ctggggcgac
     1381 aacgagccct accacgcact gcgctcggcc ttcgtgcaca agctcctgcc ggtgcgcaca
     1441 ccggactatc tctgcggcga gtgaggcaaa cgcacaacgc tcgcaagaga aacgtcaggc
     1501 tcgaaccggc cgcaccagca gctgcggccg ccgatatcag tgcaggaagc gggtgttcca
     1561 cgcgagtacc cagtgctgct gatcgtctgc gcgcacggtg atgaatgccg tgtttagctc
     1621 ttagcgacgc gggcactgct gcgatgcacg gacctcttat taggccgggc cgtgcctgac
     1681 gtcaccggtc aagtgtcaac tcttacatcc ccctttgggg ggatgtcgtc gccatggccg
     1741 attccctaag gtttgcgcgc tggcttcact aaagagggag aaaaccgtgg atatcaacgt
     1801 tggggatgtt cttcaagcgc gctatgacat ggtcgctgta gccacgtcat acgtggtgtc
     1861 ggtcctgggc tcttatgcag cgcttgcgca tacgccgcac atgttcaatc gcgacggatc
     1921 gctcaagtgg tcgatggtcg ttggagccgc agtggccctg ggtggcatcg gcatctggac
     1981 catgcatttc atcggcatga tcggctatca cctgccattc cgcgtggtat acgagggcac
     2041 gctgacgctg gtgtcgctcg ccgcggccat cgtcatcgcc ggcatcgcgc tcgtgctgcc
     2101 aggcctcggc ggcaggttca gcttgggcgg atgggccgtg ggcagcgtgc tggctgggct
     2161 gggggtatgc gtcatgcact acatgggcat gttcgctatg aacctgcagg ccgccatgaa
     2221 tctggacatg aagatcgtag ggctgtcggt agcgattgcg atcaccgccg cggcagcagc
     2281 attgtggctg gcatatcacg tccgcaagac cctgcatcgc gctgtcgcag ccgtagtgat
     2341 ggggctcgca gtctgtgcaa tgcattacac gggcatgtcg gccgccgagt tcatctgcgt
     2401 tgcgcagact ccggcctcgg tgtggggcgt cggcggcagc gatctgcttc ccgtggtgtt
     2461 cggcgtagcc agcgtggtgc tggcggtgct gacctggaac gtgctcggga tttttgttga
     2521 ggacggtccg agcccagtca tggcgaagcc gcgcgcctct gccttctgat cctccagtgc
     2581 ggcctgtccg gtcccgtgca cccatgtgcc ggacgggacc aagacagggg cttacttaga
     2641 acacagcttg ctcagggagc gcccaagact cagaacatag ggcttgagat aacaacaaaa
     2701 gaaaagggct tagcagtcac atcgctaagc ccttgattca tttggtggag acgaggagga
     2761 tcgaactccc gaccttcgca ttgcgaacgc gacgctctcc cagctgagct acgtccccga
     2821 agccgtgaat tctaacgcat caggcgaggc cgttgcggcg cgccagttcg acgaacagcg
     2881 cggactgatc ctgactcgcc aagccgtctg ccgctgcctc ggcatagagg ctctccagca
     2941 gctgcgtcac gggcgcggag aagccgagtt cagcggcggt ctgcagggca ttgcgcagat
     3001 ccttgagctg caccgccagc gtgccgcgct tggcgaaatc gcgctcgacc atgcgcagac
     3061 catgcacttc gagcacgcgg ctctcggcga agccgccgcg tatcgcctcg cgcaccccag
     3121 ccatgtcggc accgcccttg gcggcgaata gcagggcttc agcgacggca ccgataccga
     3181 tgccgacgat catctggttg gccagcttgg cgagctggcc cgcgccatgc ggcccgacat
     3241 gggtggcccg cccgaggtgc tggaacaagg gccgtgcacg ctcgaaatcg gccggctcgc
     3301 cacccaccat gatggccaga gtgccagcct cggcgcccag cgtgccgccg gacaccggcg
     3361 cgtccagatg ggccatgccc agcgcagcga gcgctgccgc atgctcgcgc gcttcgctcg
     3421 gcttgatcga gctcatgtcg atcacgagcg cgccgcgctg caaggccgtt gcagccccct
     3481 gctcaaacag cacgtcgcgc acgacgccgc catgctccag catggtgacg acgatgtccg
     3541 cgcccagcac ggcctgtgcg gggctgccgc ccacgcgggc gccgtgctgc gccagggcct
     3601 gcgccttggc aggcgtgcga ttccatgccg tcagctcgaa gccggcttcg caaagacgcc
     3661 gcgccatcgg cgcacccatc aggccggtgc ccagccaggc aatcttcttc actcgcttcc
     3721 ttcctcgcgg cgccgcctgg cgcgagccag cgccgcctcg atgacggcgc gcttgcgctc
     3781 gatctgctgc ggatcgctga gccgcgtgtg ctcggccagg ctggccagct tctgttccgc
     3841 cttggtggcc aggcgttctt cctgctcccg cctggcgcgc cggcgccgct gctgttgctg
     3901 cccatagcgt tcgcgcgccg cctcggcctg ggccggcgac catgcctgcc agccgctgcg
     3961 cccgggcgtg acgtcgagca gcgagatgca atccaccggg caggccggga tgcacagctc
     4021 gcagcccgtg cactgcgcct cgatcacggt gtgcatctgc ttcggcgcgc ccacgatgca
     4081 gtccaccggg caggctttga tgcacagcgt gcaacctatg caccagttct cgtcgatgac
     4141 ggcgagcctg agcggcccct cggccccgtt caccggattc aggggcagcg gcgccttgcc
     4201 ggtgagcgcg gccagtcggg cgatgccttc ggcacctccc ggcgggcatt gattgatctc
     4261 ggcctcgccg caggcgatcg cctgcgcata gccgcggcag tccggatagc cgcagcgtgt
     4321 gcactgcgtc tgcggcagct gtgcgtcgat ctgctcgatg gtgacgacaa cggggttcac
     4381 agcccggatt gtcgcaaggc agcagccccg cgccccggat cgccggccgc agccccttga
     4441 tgacggctac ttgtcgtact gcgcgatgaa gtcgcgaatc tcgggatagg ccttctcgcg
     4501 ccagcggcgg cccgagaaga tgccgtagtg gccagcgccc ttgacagagt agtggcgctg
     4561 tctagtcttc gcgatgcccg tgcacaggct gtgcgccgcc tcggtctggc cggcgcccga
     4621 gatgtcgtcc agctcgccct cgaccgtcag caaggccgtg cccttgatgt cctgagggcg
     4681 caccggctgg ccgcccacct tccagctgcc gttgaccagc gcgaaatcct ggaacacggt
     4741 cttgatggtg tcgaggtagt actcggccgg catgtcgagc acggcgttgt attcgtcgta
     4801 gaactcgcga tgtgcttcgg catcctcgag atcgccgcgc aacagatcct cgtaaaaatc
     4861 gtagt
//
LOCUS       DASUCY010000056         2236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_643807, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000056 DASUCY010000000
VERSION     DASUCY010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643807"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VFV25_01180"
     CDS             <1..531
                     /locus_tag="VFV25_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEY1391955.1"
                     /translation="SGREVMAEFRRATGLPTATNMIATDWRQLSHALTLQSVDIPLAD
                     PHFWTMAGSVRVAQTCRDWGLTWGSHSNNHFEVSLAMFTHVAAAAPGKVTAIDTHWIW
                     QDGQRLTREPLRIREGLIEVPKKPGLGIELDMVEVEKAHRLYLEHGLGARDDAKAMQY
                     LIPGWSFDPKRPALDR"
     gene            616..1974
                     /locus_tag="VFV25_01185"
     CDS             616..1974
                     /locus_tag="VFV25_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004633974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1391956.1"
                     /translation="MDMAQATSVDDAAAGRRTRTRYFIVAMLFIVTAINYADRATLSI
                     AGPALSKDLGLDAVTMGYVFSAFGWSYVIGQLPGGWLLDRFGSKSVYFASILLWSLFT
                     MLQGGVSFLTATAASAVVVLFALRLLVGAAEAPSFPANGRIVAAWFPANERGTASAVF
                     NSAQYFATVLFAPIMGWITHAYGWPYVFYFMGAVGIVAGLAWLKFVHSPKDHPGINAA
                     ELDYLKQGGALVDMDRSGKAASAADKPKWSYIKQLLGNRMLLGVYIGQYCITTLTYFF
                     LTWFPVYLVKERGFSILNAGFVAALPAVCGFVGGVLGGLISDYLLHKGKSLTVSRKTP
                     IVIGMLLSTSMIACNYVDSAALVVGIMAFAFFGKGLGALGWAVVSDTSPKEIAGLSGA
                     LFNTFGNTAAITTPIVIGYLVKGTGSFGAALVFVAANALIAVISYLFVVGEIKRVVLA
                     KD"
     gene            2008..>2236
                     /locus_tag="VFV25_01190"
     CDS             2008..>2236
                     /locus_tag="VFV25_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEY1391957.1"
                     /translation="MRPNKLREIWQSGGGAVNGWLAIPNSFSAEIMAHQGWDTLTIDL
                     QHGVVDYQAMVTMLQAISTTPTVPVVRVPWLE"
BASE COUNT          359 a          780 c          700 g          397 t
ORIGIN      
        1 tcgggccgcg aggtcatggc cgagttccgc cgcgcgaccg gcctgcctac ggccacgaac
       61 atgatcgcca ccgactggcg tcagctgtcg cacgcgctga cgctgcagtc ggtggacatt
      121 ccgctggccg acccgcactt ctggaccatg gccggctcgg tgcgcgtggc gcagacctgc
      181 cgcgactggg gcctgacctg gggctcgcat tccaacaacc atttcgaggt atcgctggcg
      241 atgttcaccc acgtcgccgc cgccgcgccg ggcaaggtca ccgcgatcga cacccattgg
      301 atctggcagg acggacagcg cctgacgcgc gagccgctgc gcatccgcga gggcctgatc
      361 gaggtgccga agaagccggg cctgggcatc gagctcgaca tggtcgaggt cgagaaggcg
      421 caccggctct acctcgagca tggcctcggg gcgcgcgacg acgccaaggc gatgcagtac
      481 ctcatcccgg gctggagctt cgatcccaag cggccggccc tggaccgctg acgagtacga
      541 gatccccatc cccgccatcc ccgccccgaa cgaattcaaa acagccgcaa gcgagcaagc
      601 ggcaaaggag acaacatgga catggcccag gccacttccg tcgacgacgc cgccgccggc
      661 cgccggaccc gaacgcgcta tttcatcgtg gcgatgctct tcatcgtcac ggcgatcaac
      721 tatgcggacc gcgccacgct gtcgatcgcc gggccggcgc tgtccaagga tctggggctg
      781 gacgccgtca cgatgggcta tgtcttctcg gccttcggct ggtcctacgt catcggccag
      841 ctgccgggcg gctggctgct cgaccgcttc ggctcgaagt cggtctactt cgccagcatc
      901 ctgctgtggt cgctgttcac gatgctccag ggcggcgtca gctttctcac cgcgaccgcc
      961 gcgtcggccg tggtcgtgct gttcgcgctg cgcctgctcg tgggcgcggc agaggcccca
     1021 tccttcccag ccaacgggcg catcgtggcg gcctggttcc cggcgaacga gcgcggcacc
     1081 gcctcggccg tcttcaactc ggcccagtat ttcgcgaccg tgctgttcgc gccgatcatg
     1141 ggctggatca cgcacgccta cggctggccc tacgtgttct atttcatggg cgccgtgggc
     1201 atcgtcgccg gcctcgcctg gctcaaattc gtccacagcc ccaaagatca tccgggcatc
     1261 aacgcagccg agctggacta tctcaagcag ggcggcgccc tggtcgacat ggaccggtcc
     1321 ggcaaggccg cttcggctgc cgacaagccc aagtggtcct acatcaagca gctgctgggc
     1381 aaccgcatgc tgctgggcgt ctacatcggc cagtactgca tcacgaccct gacctatttc
     1441 ttcctgacct ggttcccggt ctacctcgtc aaggagcgcg gcttctccat cctcaacgcc
     1501 ggcttcgtcg cggcgctgcc ggcagtctgc ggcttcgtcg gcggcgtgct cggcgggctg
     1561 atctccgact acctgctgca caagggcaag tcgctgaccg tgtcgcgcaa gacgcccatc
     1621 gtcatcggca tgctgctgtc gaccagcatg atcgcctgca actatgtcga ttcggctgcc
     1681 ctggtcgtcg gcatcatggc cttcgccttc ttcggcaaag ggctgggcgc actcggctgg
     1741 gcggtggtgt ccgacacctc accgaaggaa atcgccggcc tgagcggcgc gctgttcaat
     1801 actttcggca acacagcggc gatcacgacg cctatcgtga tcggctatct cgtcaagggc
     1861 acgggttcct tcggcgcggc actggtgttc gttgctgcca atgcgctgat cgcggtgatc
     1921 agctatctct tcgtggtcgg cgagatcaag cgcgtggtgc tggccaagga ctaagacctg
     1981 cccgaccgaa acctgaagga gtacacgatg cgtcccaaca agctgcgcga aatctggcaa
     2041 tccggcggcg gcgccgtcaa cggctggctg gccatcccga acagcttctc cgccgagatc
     2101 atggcccacc agggctggga cacgctgacc atagacctcc agcatggcgt cgtggactac
     2161 caggccatgg tgaccatgct gcaggcgatc tcgaccacgc ccaccgtgcc ggtggtgcgc
     2221 gtgccctggc tcgagc
//
LOCUS       DASUCY010000057         2098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1248, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000057 DASUCY010000000
VERSION     DASUCY010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1248"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VFV25_01195"
     CDS             <1..285
                     /locus_tag="VFV25_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY1391958.1"
                     /translation="IIAQQMESGIVFALGAALYSRIDIVNGAVQQNNFPNYPLLSLRE
                     TPVIETHILPSTLPPTGVGEPGTPPVAPALANAIFVLTGQRLRELPLKLS"
     gene            complement(278..892)
                     /locus_tag="VFV25_01200"
     CDS             complement(278..892)
                     /locus_tag="VFV25_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEY1391959.1"
                     /translation="MKLIGSLTSPYVRKVRIVLAEKRLDYQFELEDVWEPGSSITASN
                     PLGKVPCLVMEGGEAVFDSRVIVEYVDTLSPVGKLIPASGRERVEVRTWEALADGLMD
                     AAVLARLEQTWGGRTELQRSPAWVTRQMDKVHAAIDAMAHGLGERPWAVSNHFSLADI
                     AVGCALGYLDFRFAHIDWRGQHPNLARLYEKLVQRPSFTETAPS"
     gene            1068..>2098
                     /gene="purB"
                     /locus_tag="VFV25_01205"
     CDS             1068..>2098
                     /gene="purB"
                     /locus_tag="VFV25_01205"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEY1391960.1"
                     /translation="MTSAALSALTALSPLDGRYAAKVAALRPLLSEFGLMHRRVQVEV
                     EWFIALSDAGLPEFAPLSAAAREQLRALVSGFAEADAQAIKDIEKTTNHDVKAVEYWI
                     KSRFEGQPELQKAGEFVHFACTSEDINNTSHGLMLKAAREQVLLPALDGLVATLSGMA
                     QRFAALPMLSRTHGQTASPTTVGKEIANVVARLSTARERIASVKLLGKMNGAVGNYNA
                     HLAAYPEVDWEAFSRRVVEQQLGLSFNPYTIQIEPHDYMAELFDAVTRANTILIDWSR
                     DAWGYISLGYFKQKTKAGEIGSSTMPHKVNPIDFENAEGNLGLANALLTHLSQKLPIS
                     RWQRDLTDST"
BASE COUNT          379 a          743 c          650 g          326 t
ORIGIN      
        1 atcatcgccc agcagatgga aagcggcatc gtcttcgccc tcggcgccgc gctctacagc
       61 cgcatcgaca tcgtgaacgg cgccgtgcag cagaacaact tcccgaacta tccgctgctg
      121 agcctgcgcg aaacacccgt catcgagacc cacatcctgc ccagcaccct gccgccgacc
      181 ggcgtcggcg agccgggcac accgccagtg gcgccggcgc tggcgaatgc gatctttgtg
      241 ctgacggggc agcgcttgcg cgagctgccg ttgaagctca gctaggcgcg gtctcggtga
      301 agctggggcg ctgcaccagc ttttcgtaca ggcgcgccag attgggatgc tggccgcgcc
      361 agtcgatgtg ggcaaagcgg aaatcgaggt agcccagcgc acagccgacc gcgatgtcgg
      421 ccagcgagaa atggttgctg accgcccagg gccgctcgcc caggccgtgc gccatcgcat
      481 cgatggcggc atgcacctta tccatctgcc gcgtcaccca ggccggcgag cgctgcagct
      541 cggtgcggcc gccccaggtc tgctccaatc gagccagcac agcggcgtcc atcaggccgt
      601 cggccaaggc ctcccaggtg cgcacctcga cgcgctcgcg ccccgaggcc gggatcagct
      661 tgcccaccgg cgacagcgta tcgacgtact cgacgatgac gcgcgaatcg aacaccgcct
      721 cgccgccctc catcacgagg cagggcacct tgcccagcgg attcgaggcc gtgatgctgc
      781 tgcccggctc ccagacatct tccagctcga actgatagtc cagccgcttc tcggccagga
      841 cgatgcgaac cttgcgcaca taggggctgg tgagcgagcc gatcagtttc atgttgtttg
      901 tactgccatt gtttgatttc attctagcga gcgcataagg cggcgacacc ccagtgcggg
      961 ggagtgcgcc acagtccgac gcgtggcgcg gcggcacagg ctgggcctcg aagcgagtgc
     1021 ctacaattca gggctttccc tcagcaacat ccagttcgcg cactgccatg acctccgccg
     1081 ccctgtccgc tctcaccgcc ctgtcgccgc tggacggccg ctatgccgcc aaggtcgccg
     1141 cgctgcgccc gctgctttcg gaattcggcc tcatgcaccg ccgcgtgcag gtcgaggtcg
     1201 agtggttcat cgcgctgtcc gatgcggggc tgcccgagtt cgcgccgctg tcggctgccg
     1261 cacgcgagca actgcgcgcg ctggtgagcg gcttcgccga ggccgacgca caagccatca
     1321 aggacatcga gaagaccacc aaccacgacg tcaaggccgt cgagtactgg atcaagtcca
     1381 ggttcgaagg ccagcccgag ctgcagaagg ccggcgagtt cgtgcacttc gcctgcacct
     1441 cggaagacat caacaacacc agccacggcc tgatgctcaa ggccgcgcgc gaacaggtgc
     1501 tgctgcccgc tctcgatggc ctcgtcgcca cgctgtccgg catggcgcag cggttcgccg
     1561 cgctgcccat gctcagccgc acgcacggcc agaccgcgag ccccactacg gtgggcaagg
     1621 agatcgccaa tgtcgttgcg cgcctgtcca cggcgcggga gcgcattgcc agcgtgaagc
     1681 tgctgggcaa gatgaacggc gccgtaggca actacaacgc ccacctcgcg gcctaccccg
     1741 aggtcgactg ggaagccttc agccgccgcg tggtcgagca gcagctgggc ctgagcttca
     1801 acccctacac gatccagatc gagccgcacg actacatggc cgagctgttc gacgcggtca
     1861 cgcgcgccaa caccatcctg atcgactggt cgcgcgacgc gtggggctac atcagcctgg
     1921 gctatttcaa gcagaagacc aaggccggcg agatcggcag ctcgaccatg ccgcacaagg
     1981 tcaatccgat cgacttcgag aatgctgaag gcaacctcgg gctggccaat gcgctgctca
     2041 cgcatctgag ccagaagctg ccgatcagcc gctggcagcg cgacctgacc gactccac
//
LOCUS       DASUCY010000058         3780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1302371, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000058 DASUCY010000000
VERSION     DASUCY010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1302371"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="VFV25_01210"
     CDS             <1..329
                     /locus_tag="VFV25_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HEY1391961.1"
                     /translation="RELGADVHAAEQERQHVEPVGLHEVADQKDEATQSQMAESDSAQ
                     EQLERDELAEVDAALRRLDDGTYGDCAGCGNSIPLQRLMVQPAALRCAACQAAYECNR
                     ELRPPH"
     gene            complement(564..3239)
                     /locus_tag="VFV25_01215"
     CDS             complement(564..3239)
                     /locus_tag="VFV25_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HEY1391962.1"
                     /translation="MTIRHLDRLLEPASVAVFGASDRAGRVGTTVWNNLHGRFRGALY
                     AVNPSHAQLGRVPVFASAKDMPSTPDLAVLCTPPATIAPLVAELAALGTRAAVIVTAG
                     LSPAQKQAALDAARPKLLRLLGPNCIGVLTPRIGLNASFAHAGALPGSLAFVSQSGAL
                     LTAVLDWANSRHIGFSHLVSLGEHCDVDFGDLLDHLANDAHTRAILLYIESVQAPRKF
                     MSAARAAARNKPVIVVKAGRAGHGVQAAASHTGAMAGADIVFDAAFRRAGMLRVDTLQ
                     ELFVAAETLARFSGNRDERLSIMTNGGGAGVMAADAAALRGVALADPPASVLQQLDVE
                     LPANWSHGNPIDIIGDAPVERYTATLSALLAEPAAGAVLFLHAPTAIVNSQAIARACL
                     PLIQAAPQRVMSCWLGDGAVAAARQLFEEAGVPDYPTPEDAVHAFEMLQTYRRNQETL
                     MEAPATAGNAAPDAGAALDIVSAALAQRREWLTESESKAVLAAYGVPVVPTLAVEATP
                     EAALAAAQSLGFPVALKILSPDITHKTDVGGVRLDLHDADAVARAAADMLETVRRAKP
                     DASVTGFTVQPMARRPHAQELIVGASIDAVFGPVILCGQGGTAVEVMADRAVALPPLN
                     RSLAHELLARTRVSRLLAGYRDHPPAKLDALYDVLIAVSQMLADLPQLAELDINPLWV
                     DEDGVLALDARIRLAPAPVAGAERFAILPYPEQWVRNLRWQGREITLRPIRPEDEAQH
                     RRFLECLAPEDVRMRIFYTRRDLPHSELARLTQIDYDREMAFIAVGTNAQGAPQTLGV
                     VRAIRDPDNIEAEFGVIVRSDLKGLGLGRVLLDELISHVRASGTQRLVGLVLRENRSM
                     LALARAVGFDADPVEPPQSDFVRLVLPLQAQTVPA"
     gene            3456..>3780
                     /locus_tag="VFV25_01220"
     CDS             3456..>3780
                     /locus_tag="VFV25_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="HEY1391963.1"
                     /translation="MDGQAAESVPGQSLARALQRGLEQQTGQAVAVKETHISWVLLTG
                     QLAFKLKKPVRLPFLDFGSIAARKHWCEEELRLNRRTAPGLYLSVLPVRGAADAPRIA
                     GKGPVI"
BASE COUNT          591 a         1267 c         1379 g          543 t
ORIGIN      
        1 aacgggaatt gggtgccgac gtgcatgcgg cggaacagga gcggcagcac gtagagcctg
       61 tgggcctgca tgaggttgcc gaccagaagg acgaggcgac acagtcgcag atggccgagt
      121 cggacagcgc gcaggagcaa ctcgagcgtg acgagctggc cgaagtcgac gccgccttgc
      181 ggcgcctgga cgacggcacc tacggcgatt gcgccggctg cggcaactcc attcctttgc
      241 agcggttgat ggtgcagccg gccgcgctgc gctgtgcagc ctgccaggcc gcctacgaat
      301 gcaaccggga gctcaggccg ccccattgaa gtgggcggag acggaccacc gagatggccg
      361 caagccacag gcacgcctaa cgcacgtgag tccgtgccta gtccggcgcc cgacagggcg
      421 cgcgtgggat gcgcagcgca agagcagggt gagctggagt tgggccgcct ttggcgggcg
      481 agttgcgccc tgaagccggg gggcgcttgg atggctggcg ctgccgcgtg gcagccgtgt
      541 cgccacctcg ttgaagcatt aaatcaggcc ggcactgtct gcgcctgcag cggcaggacc
      601 agccgcacga aatcggactg cggcggctcg acggggtcgg catcgaagcc gacggccctg
      661 gcaagcgcca gcatcgagcg gttctcgcgc agcacgaggc ccaccagccg ttgcgtgccg
      721 ctcgcgcgca cgtggctgat gagctcgtcg agcagcaccc ggccgaggcc caggcccttg
      781 agatcggagc gcacgatgac gccgaactcg gcctcgatgt tgtccgggtc gcggatggcg
      841 cgaaccacgc ccagcgtctg cggcgcaccc tgcgcgttgg tgccgacggc gatgaatgcc
      901 atctcgcggt cgtagtcgat ctgggtcagc cgggccagct cgctgtgcgg caggtcgcgg
      961 cgcgtgtaga agatacgcat gcgcacgtct tcgggcgcca gacattcgag gaagcggcgg
     1021 tgttgcgcct cgtcctcggg gcggatcggg cgcagggtga tttcgcggcc ctgccaccgc
     1081 aggttccgca cccattgctc ggggtagggc aggatggcga agcgctcagc gccggccacc
     1141 ggcgcgggcg ccaggcggat gcgggcatcc aacgcgagca cgccatcctc gtcgacccac
     1201 agggggttga tatccagctc ggccagctgc ggcaggtcgg ccagcatctg cgatacggcg
     1261 atgagcacgt cgtacagcgc gtcgagcttg gcgggcggat ggtcccggta gccggccagc
     1321 aggcgcgata cccgcgtgcg cgccagcagc tcgtgggcca ggctgcggtt cagcggcggc
     1381 agcgctacgg ctcggtcggc catgacttcc accgccgtgc cgccctggcc gcacaggatc
     1441 accgggccga acaccgcgtc gatgctggcg cccacgatca gctcctgcgc atgcggccgc
     1501 ctggccatgg gctgcaccgt gaagcccgtg acgcttgcgt cgggcttggc gcgacgcacc
     1561 gtttccagca tgtctgcagc ggcgcgcgcc acggcatcgg cgtcgtgcag atccaggcgt
     1621 acgccgccca cgtccgtctt gtgcgtgatg tcgggtgaca ggatcttcag tgccaccggg
     1681 aagcccagcg attgcgcggc agcgagcgcg gcctcgggtg tcgcttcgac cgccaatgtc
     1741 ggcaccaccg gcacgccgta tgcggcaagc acagccttgg actcgctctc agtgagccat
     1801 tcacgtcgct gtgccagcgc cgcggagacg atgtcgagcg ccgcgcccgc atccggggcc
     1861 gcattgcccg cggtcgccgg cgcctccatc agcgtttcct ggttgcgccg gtaggtctgc
     1921 agcatctcga aggcatgcac cgcatcttcc ggcgtcggat agtccggcac gccggcttcc
     1981 tcgaacagct gccgcgcggc cgcgaccgcc ccgtcgccga gccagcagct catcacgcgc
     2041 tgcggtgccg cctgaatcag cggcaggcag gcgcgtgcga tggcctggct gttcacgatc
     2101 gcagtcgggg cgtgcaggaa cagcaccgcg ccggctgcgg gctcggccag tagcgcggac
     2161 agggtcgccg tgtagcgctc gaccggtgca tcgccgatga tgtcgatcgg gttgccgtgc
     2221 gaccagttgg cgggcagctc gacgtccagc tgctgcagaa cggaggcggg tggatcggcc
     2281 agcgcgacgc cgcgcagcgc cgccgcatcg gcggccatga cgccggcgcc gccgccgttg
     2341 gtcatgatgc tgagtcgctc atcgcggttg ccgctgaagc gcgccagcgt ctctgcggcg
     2401 acgaagagct cctgcagcgt gtccacgcgc agcatgccgg cgcgccggaa ggctgcgtcg
     2461 aaaacgatat cggcgcccgc catggccccc gtatgcgagg cggcggcctg cacgccgtgc
     2521 cctgcacggc cggccttgac gacgatcacc ggcttattgc gcgccgcggc acgcgcggcc
     2581 gacataaact tgcgcggcgc ctgtaccgac tcgatgtaca gcaggatggc gcgcgtgtgc
     2641 gcgtcgttcg ccaggtggtc cagcaggtcg ccgaaatcca catcgcagtg ctcgccgagc
     2701 gacaccaggt gtgaaaagcc gatgtgccgg ctgttggccc agtccagcac tgccgtcagc
     2761 aacgcacccg attgcgacac aaaggccagt gagccgggca gggcgcccgc gtgcgcaaag
     2821 ctggcgttca ggccgatgcg cggcgtgagc acgccgatgc agttggggcc cagcagacgc
     2881 agcagcttag ggcgtgcggc atccagtgcc gcctgcttct gagcggggct cagcccggcg
     2941 gtgacgatca cggcggcgcg agtacccagc gcggcgagct cggccaccag cggcgcaatg
     3001 gtcgccggcg gcgtgcacag caccgccagg tccggcgtgg agggcatgtc cttcgcactg
     3061 gcgaagacgg gcacgcgtcc gagctgggcg tggctcggat tcacggcata cagcgcgccg
     3121 cggaagcggc cgtgcaggtt gttccacacg gtggtgccga cgcggccggc gcgatcggaa
     3181 gcaccgaaca cggccacgct ggcgggctcc agcaggcggt cgaggtgtcg gatggtcatg
     3241 ccgcgcatcc tcgtgcaggg tcagagagag cgcttgagcc atgtcaagga agcatcgggt
     3301 gagcgccagc ggaggccgcg cgccccctta ggtcacgggt ggcgcgctgc ggccggtgat
     3361 gaagacgcgc aggttcatga cagcagggag gcgcacctgt cgcttccctt gcggcgagtc
     3421 aagtcgccag ccagcaccgg gcgcagactg agcgcatgga tggacaggcc gccgaatccg
     3481 tgccgggcca atcgctggcg cgggcgctgc agcgcggcct cgaacagcag acgggccagg
     3541 ctgtggcggt gaaggagact cacatctcct gggtgctgct gaccgggcag ctggccttca
     3601 agctgaagaa gccggtgcgc ctgcctttcc tcgacttcgg cagcattgcg gcacgcaagc
     3661 actggtgcga ggaggaactg cgcctgaatc gccgcaccgc gcccgggctg tacctgagcg
     3721 tgctgccggt tcgtggcgcg gccgatgcac ccaggatagc cggcaagggg cccgtgatcg
//
LOCUS       DASUCY010000059         3466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_643820, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000059 DASUCY010000000
VERSION     DASUCY010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643820"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..1666
                     /gene="dacB"
                     /locus_tag="VFV25_01225"
     CDS             212..1666
                     /gene="dacB"
                     /locus_tag="VFV25_01225"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEY1391964.1"
                     /translation="MLSVLCRASLCVLLAVSSALACAAGLPPAVAKALKQAGLPQDAM
                     VTMVAEAGRPGKPRLAYRAEQLVNPASLMKLFTTYAALDLLGPAYSWTTPVYLDGRIV
                     NPGPAGVLEGSVYIQGRGDPTLVLERVWLLVQRLRQAGVREIRGDIVLDRSAFSVPEA
                     DPGDFDGEPYRPYNVRPDALLFNFKSLNLSFEPDVPAGVARISIEPSLAGLHIDESVA
                     LANGPCNDWRSALKADFNDPARIRFGGSFAATCGDRQWPLAYADPRSYNARLLIALWQ
                     GVGGLLSGQVREGTVPPGLKPAFEFGSPPLADAVRGINKFSNNTMAQQLFLTLGLTQR
                     GTGSFEAAREVVAAQAQERVGCSTDELMLDNGAGLSRDGRVSARCMARLLQAAWGSAV
                     MPELLSSLPVAGVDGTTKQPDRAWGRAQGRAHLKTGSLRDVTGLAGVVLGASGRRYVL
                     VAIVNHPNAAAARPALDALVQWTAADGSAPRRRP"
     gene            complement(1762..2442)
                     /locus_tag="VFV25_01230"
     CDS             complement(1762..2442)
                     /locus_tag="VFV25_01230"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HEY1391965.1"
                     /translation="MRNKQLLACALLGLAATGASAQHTVYLGGAYVDVHSSAPPLSFN
                     PAQPTTTDARVRVGSASTVGFGYSYRFNDAWSVEVALGIPPTHKTYGEGQLQGFGQIN
                     AMKQGGPTVFANYHFGEIAPHLRPFVGLGINYTRFYDSHTTTAGNWAAGGPTTTKLSD
                     SWGLAGHVGASYQMTKEWSIIGTIAAAQVKSDSTATTQLVGGGTSVATTTINFHPVVY
                     TLSVGYSF"
     gene            complement(2477..>3466)
                     /locus_tag="VFV25_01235"
     CDS             complement(2477..>3466)
                     /locus_tag="VFV25_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1391966.1"
                     /translation="QGMIVVAPNYLGYDKSSLTYHPYLNAQVQATDMIDGLRAAKNYL
                     AGSTSPIKPTDQLLITGYSQGGHVAMATHQLIEASYSSEFKVTASGPMSGPYNLAKFT
                     TVINASPMNPNPTGLTPCPSTNVNCWVDAGGTLFTPLLLTSYQKAYGNVYTSPSQAYQ
                     SPYDKTAETLFPTDTPISTLVQQGELPADPTFTKLFGTGGLINEAFRASFFTDPTNGF
                     KADVVKNTLLGWNPHRPMMLCGGAQDPTVYYFNTTDAQADFASRGVSVPAVDMENAAT
                     VPGGATGPLYLGFQAQKAAAGANVLAVYHGELVPPFCTAIVRGFFQQVLASGI"
BASE COUNT          547 a         1124 c         1191 g          604 t
ORIGIN      
        1 gcgattcccg cgtccgccag cagcttcaaa atgccgggca gcaattcggc ggaggcccgt
       61 gccccgcctt cggcttcgac gctgtggagt tcctcgcgca gcagcaggcc gagatgcatg
      121 acctcggtgg cggtatccag cacaagcaga cggggggcgc tcatgccggc agtttagcca
      181 gcgcccgcaa ggccgcccgt atcatcggcc catgctttca gtgttatgcc gcgccagcct
      241 gtgcgtgctg ctggcggttt catccgccct ggcctgcgcg gccggcctgc cgcctgccgt
      301 ggcgaaggcg ctaaaacagg ccgggctgcc gcaggacgcc atggtgacca tggtggccga
      361 agcgggccgg cccggaaaac cgcgcctggc ctaccgggcc gagcagctgg tgaaccccgc
      421 ttccttgatg aagctgttca ccacttacgc tgcgctggac ctgctcggcc ccgcctacag
      481 ctggacgacg cccgtgtatt tggacggccg catcgtcaac cccggacctg ctggcgtgct
      541 cgagggctcg gtctacatcc aaggccgcgg cgatcccacg ctggtgctgg agcgggtctg
      601 gctactggtg cagcggctgc gtcaggccgg cgtgcgcgag atccgcggcg atatcgtgct
      661 cgatcgcagc gccttcagcg tgcccgaggc cgatcccggc gacttcgacg gcgagcctta
      721 tcggccctac aacgtgcggc ccgatgccct gctgttcaac ttcaagtcgc tgaacctgag
      781 cttcgagccc gatgtcccgg caggcgtcgc gcggatcagc atcgaaccct cgctggccgg
      841 cctgcacatc gacgaaagcg tcgcgctcgc caacggcccc tgcaacgatt ggcgcagcgc
      901 gctcaaggcc gacttcaacg acccggcgcg catacgcttc ggcggcagct ttgctgcgac
      961 ctgcggcgac aggcaatggc cgctcgccta tgccgatccg cgcagctata acgcgaggct
     1021 gctgatcgct ctgtggcagg gcgtgggcgg cctgctgagc ggccaggtgc gcgaaggcac
     1081 ggtgccgccg ggcctgaagc ctgccttcga attcggctcg ccgccgctgg ccgatgcggt
     1141 gcgcggcatc aacaaattca gcaacaacac catggcccag cagctgttcc tcactctggg
     1201 cctcacgcag cgcggcacgg gcagcttcga ggcggcgcgc gaagtcgtcg cggcacaggc
     1261 acaggagcgt gtaggctgtt caaccgacga gctgatgctc gataacggcg caggcctgtc
     1321 gcgcgacggc cgtgtcagcg cgcgctgcat ggccaggctg ctgcaggcgg cctggggcag
     1381 cgccgtgatg ccggaactgc tcagctcgct gccggtggcc ggtgtggacg gcacgacgaa
     1441 gcagccggac cgcgcctggg gccgggcgca gggccgcgcc cacctcaaga ccggctcctt
     1501 gcgcgatgtg acagggctgg cgggcgtggt tctgggggct tccggccggc gttatgtcct
     1561 ggtcgccatc gtgaaccacc ccaatgccgc tgcggcccgc ccggcgctcg atgcgctggt
     1621 gcaatggacg gcggccgatg gttctgcgcc ccgccgcagg ccttgattcc gggccgatct
     1681 gaccgcacca gccatgaaaa agccggctgc acgcgcaatg cgcgacacag ccggctcggt
     1741 ttcgctgaaa cgcggctcgt cttagaagct gtagcccacc gacagcgtgt agaccaccgg
     1801 gtggaagttg atggtggtcg tcgccaccga agtgccgccg ccgaccagct gcgtcgtggc
     1861 cgtggagtcg ctcttgacct gggccgccgc gatggtgccg atgatcgacc attccttggt
     1921 catctggtag ctcgcaccca cgtgccccgc caggccccag gaatccgaca gcttggtcgt
     1981 ggtcggaccg cccgcggccc agttccccgc ggtcgtggtg tggctgtcgt agaagcgggt
     2041 gtagttgatg cccagcccca cgaagggacg cagatgcggc gcgatctcac cgaagtggta
     2101 attcgcgaag acagtcggcc cgccctgctt catcgcgttg atctggccga agccttgcag
     2161 ctggccctcg ccataagtct tgtgggtggg cggaatgccc agcgccacct cgacgctcca
     2221 ggcatcgttg aagcggtagg agtagccgaa gcccacggtc gaggcgctgc ccacgcggac
     2281 gcgggcgtcg gtcgtcgtcg gctgggccgg attgaagctc agcggcggcg cactggagtg
     2341 gacgtcgaca taggcccctc ccaggtacac ggtgtgctgg gcgctggcgc ccgtggcggc
     2401 caggcccagc agggcacagg cgagcaattg cttgttcctc attggtgatc tctccttatt
     2461 gctcggtgtc gctgcgtcag atgccgctgg ccagcacttg ctggaagaag ccgcgcacga
     2521 ttgcggtgca gaagggcggc acgagctcgc cgtggtaaac cgcgagaacg ttggcaccgg
     2581 cggccgcttt ttgcgcctgg aagcccaggt acaagggacc ggtggcccca cccgggacag
     2641 tggcagcgtt ctccatgtcc acggcaggca cgctcacgcc gcgcgaggcg aaatcggctt
     2701 gggcatcagt cgtgttgaag tagtacaccg tggggtcctg ggcgccgcca cacagcatca
     2761 ttggacgatg aggattccag ccgagcagcg tgttcttcac cacatccgcc ttgaagccgt
     2821 tggtcggatc ggtgaagaag ctggcgcgaa atgcctcgtt gatcaggccg ccagtgccga
     2881 acagcttggt gaaggtcggg tcggcaggca actccccttg ctgcaccagc gtcgaaatcg
     2941 gcgtgtcggt cgggaacagg gtctcggccg tcttgtcgta gggcgactga taggcttggg
     3001 atgggctggt gtagacgttg ccgtaggcct tctggtagct ggtcaacagc aatggcgtga
     3061 acagcgtccc gcccgcatcc acccagcaat tgacattggt cgacgggcag ggcgtcaggc
     3121 ccgtcgggtt cgggttcatc gggctggcgt tgataaccgt cgtgaacttc gcgaggttgt
     3181 atgggccgga catcggcccc gaagccgtga ccttgaactc gctggagtaa ctggcctcga
     3241 tcagctggtg cgtggccatc gccacatggc cgccctgcga atagccggtg atcagcaact
     3301 gatccgtcgg cttgatcggg ctcgtgctgc cggccagata gttcttggcg gcgcgcaggc
     3361 cgtcgatcat gtcggtggcc tggacctggg cgttgagata agggtgatag gtgaggctgg
     3421 acttgtcgta gcccaggtag ttgggcgcga cgacgatcat gccctg
//
LOCUS       DASUCY010000060         2719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_33358, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000060 DASUCY010000000
VERSION     DASUCY010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33358"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VFV25_01240"
     CDS             <1..256
                     /locus_tag="VFV25_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEY1391967.1"
                     /translation="TLEVCLTFSAAGPPRFEELAAQAESRGYRVLRDSLRISFADSQP
                     MWRFCVVALDRTRAASPDSLAQDMATAEGVASFSIVPVRN"
     gene            265..1707
                     /locus_tag="VFV25_01245"
     CDS             265..1707
                     /locus_tag="VFV25_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY1391968.1"
                     /translation="MNAALYAARRVALVIVLGLLGACSTTRPWVNQPLAPSARLESTP
                     ALNPMLPVGAPPGSPQVIAAVALSGGGARAAAFGLGVLEELKATRFALGGRSTTLLDE
                     VGLVSGVSGGSVLATYYAAFGDEVFTRFEPEFLKADFQSSLIGYILSPASLYRLTSPW
                     YGRSNVLAERLAPVFRGLTFGDLKARPRGPRLLITAADLTTGAPFEFTPEQFALICSD
                     LDSMPLSFAAAASSSVPLLLSPITLRNYAGSCPQSAQVVEAAMADHNLSARLLRTIAK
                     SYQNSSARPYLHLVDGGLVDNLGVRSLLDHTIARGSLRQSFEHLPPGSVHRVVLVSVN
                     SERDLGDRLDDSGRVPSTVQVLDALVFGAGSRTTTETSAMVVDAARRLAEELVADRGS
                     AGSPFAADAQLHVISVSLRDVPDEALRQQLLQVPTAFTILPLQVRQLREAGRSALRAS
                     AEFQKLVQGLQGQDAPMSIAGNANAANSTD"
     gene            1806..2114
                     /locus_tag="VFV25_01250"
     CDS             1806..2114
                     /locus_tag="VFV25_01250"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY1391969.1"
                     /translation="MSALVTSDRVRVRQIVSTLLSNAVKFTSRGSVHLRATMTAKGQV
                     RLAVADTGIGIEAEVLARLFRPFVQAGESTTRQYGGSGLGLLICRALANDGWPGGCSE
                     "
BASE COUNT          408 a          960 c          921 g          430 t
ORIGIN      
        1 gacgctggag gtgtgcctca ccttcagcgc ggccggcccg ccgaggttcg aggagctggc
       61 cgcgcaggcc gaatcgcgcg gctaccgcgt gctgcgcgat agcctgcgca tcagcttcgc
      121 cgacagccag ccgatgtggc gcttctgcgt ggtggcgctg gaccgcaccc gcgccgcgtc
      181 gcccgattcg ttggcgcagg acatggccac ggccgaaggc gtggcgagct tcagcatcgt
      241 tcccgtgagg aactgacccg cgtgatgaac gctgccctgt acgccgcgcg ccgcgtcgcg
      301 ctggtcatcg tcctgggcct gctgggcgcc tgctccacca cgcggccctg ggtcaaccag
      361 ccgctcgcgc cgtcggcacg gctcgagtcc accccggccc tcaacccgat gctgccggtg
      421 ggcgccccgc ccggctcgcc gcaggtgatc gccgcggtgg cgctatccgg tggcggtgcg
      481 cgggccgcgg cctttgggct cggcgtgctg gaggaactca aggccacgcg cttcgcgctc
      541 ggcggccgtt cgaccacgct gctcgacgag gtggggctcg tcagcggcgt gtccggcggc
      601 agcgtgctcg cgacctacta cgccgccttt ggcgacgagg tgttcacgcg cttcgagccc
      661 gagttcctca aggccgactt ccagtcgagc ctcatcggct acatcctctc gccggccagc
      721 ctgtaccggc tcacctcacc ctggtacggc cgcagcaacg tgctggccga aaggctggcc
      781 ccggttttcc gcggcctgac cttcggcgac ctgaaggcgc ggccgcgcgg cccgcgcctg
      841 ctgatcaccg ccgccgacct caccaccggc gccccgttcg agttcacgcc cgagcagttc
      901 gcgctgatct gctccgacct cgacagcatg ccgctgtcgt tcgccgccgc ggcctcgtcg
      961 tcagtgccgc tgctgctgag cccgatcacc ctgcgcaact acgccggctc ctgcccgcag
     1021 tcggcccagg tggtggaagc cgcgatggcc gaccacaacc tctcggcgcg cctgctgcgc
     1081 accattgcca agagctacca gaactcgtcg gcgcggccct acctgcatct cgtggacggc
     1141 ggcctggtgg acaacctcgg cgtgcgcagc ctgctcgatc acaccatcgc ccgcggctcg
     1201 ctgcgccaga gcttcgaaca cctgccgccg ggctcggtgc accgcgtcgt gctggtcagc
     1261 gtgaactccg agcgcgacct cggcgaccgg ctcgacgaca gcggccgtgt gcccagcacg
     1321 gtgcaggtgc tggacgcgct ggtattcggc gccggctcgc gcaccaccac cgaaacgagc
     1381 gcgatggtgg tcgatgccgc gcgccgcctg gccgaggagt tggtcgcgga ccgcggcagc
     1441 gccggcagcc ccttcgcggc cgacgcgcag ctgcacgtga tcagcgtgag cctgcgcgac
     1501 gtgcccgacg aagcgctgcg ccagcagctg ctgcaggtgc ccaccgcgtt caccatcctg
     1561 ccgctgcagg tgcgccagct gcgcgaggcc ggccgcagtg cgctgcgcgc ctcggccgag
     1621 ttccagaagc tggtgcaggg cctgcagggg caggacgccc cgatgtccat cgcgggcaat
     1681 gccaacgcgg cgaacagcac ggactgatct tcgcgcgatt ccggctctca gccgggcagg
     1741 ccctcggcct tcggcgcctg ctgcaccgcc ggccgcgcgt tcatgcgggc catgaaggca
     1801 ttgaagtgtc ggcgctcgtg acaagcgacc gggtgcgcgt acgtcagatc gtctccaccc
     1861 ttttgagcaa cgcagtcaag ttcacatcgc ggggcagtgt gcatctgagg gccacgatga
     1921 cggccaaagg ccaggttcgt cttgccgttg cagacaccgg aatcggcatc gaagcggagg
     1981 tcctggcacg gctgttccgc ccattcgtgc aggccggcga atcgacgacg cgccagtacg
     2041 gaggcagcgg cctgggcctg ttgatctgcc gcgcgctggc caatgatggg tggccgggtg
     2101 ggtgctcaga gtgagcctgg acgaggcagt gtcttctggg ccgaactgcc gttggagatg
     2161 gcgcaggtgc aggcatcgcc tgccgacgct gcctgcgcgc cctgtgggtg attcatatcc
     2221 gcccgaaacc cgctagacga tcattcagca acagtgcctt gatcgccaga tcgtccggca
     2281 gcgggaccag tgggtagccc ggaggtctcg aggaaggcag gggtacggat cgcccctgcg
     2341 cgatgtaggt cccgactgcg gccgccagcc tgtgctccgc cagccacagt acgcttgtta
     2401 tggatgtccc tgaagtcacc gcctcgggga catcggggaa cgacaaatgg tatccgttgt
     2461 ctctgcgctc gacacgcgcg ggatatcgaa tcggcagcat cggcaactaa cgcctattag
     2521 atcggctcgc gagcccaacc gaacagctga cggctctcgc gccagaagac atcgacatca
     2581 ccggcatgcg gggtctgctg gtcgaagaca acgagacgaa caccgtgatc gaaagcgcca
     2641 tgatgaggcg ctggggcgtc gaagtgtcca ccgcgcaagg cggatgcgag gcgctggaac
     2701 tgatcgatca ggagcgacg
//
LOCUS       DASUCY010000061         7267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1157845, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000061 DASUCY010000000
VERSION     DASUCY010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 7267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7267
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157845"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VFV25_01255"
     CDS             complement(<1..396)
                     /locus_tag="VFV25_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY1391970.1"
                     /translation="MLQIDDLKVALDADFGLVKAIDGMRLSIQRGETFALVGESGCGK
                     SMTALALMRLLPENGRITDGRLALEGEDVLALPEARMRDVRGGRIGMIFQEPATSLNP
                     VMRVGDQIVEAIETHTSLRGPAARAKAIEW"
     gene            complement(411..1865)
                     /locus_tag="VFV25_01260"
     CDS             complement(411..1865)
                     /locus_tag="VFV25_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY1391971.1"
                     /translation="MSPKFVLLWTDVVIYILVAVTAFYAWRVAKRPNLRANWLKVLRD
                     PAALCSALVLLLFCGVTLLDSVHFRRALPPSSNGDTRVFYDTRTESLLDVALARQIAM
                     RETTYSRPLAYAGFNKDTVEVDGQSVRDYPRLKFGGAHLKDPAQEWAGDLVSRAVTGL
                     LGGLLFAGLVGAAAAGFYARHHKRGWSDAVAAILGNRTDLPWRAAFLTIVIGCLLCGP
                     IAALMNHYHVFGTDRTGNDVLLLALKSVRTAFVIGTLATLATLPFAIVLGILAGYFKG
                     WVDELIQYLYTTLSSVPNVLLIAACVLMVQVFMDKNPNLFETGAERSDLRIFLLCVIL
                     GLTGWATLCRLVRAETLKLRELEYVQAATAFGVSHARIMARHVFPNLVHLVLITTVLS
                     FSDLILYEAVLTYVGVGVDPSMSSFGGMINLARSEMSRDPVVWWSFAAAFFFMVTLVL
                     AANLFADGVRDAFDPRSRAFRPRLAAMPKPAAIK"
     gene            complement(1995..2978)
                     /locus_tag="VFV25_01265"
     CDS             complement(1995..2978)
                     /locus_tag="VFV25_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY1391972.1"
                     /translation="MLNYIIRRIGYGVLILIGVNLFTFFLFFTVNTPDDMARLNIGGK
                     RVTQEQIEKWKAERGYDKPLYWSAKESGAAKLTDTIVWDRSVSLFKLDFGRADSKNAV
                     DIGYEIKTRMGVSLQLALPLFILQVIISVAFSLMLVFFRHSRIDFWGVVLCVLMLSIS
                     SLFYIIVGQFVFSRVFRLAPISGYAGGLDAAKFLVLPIALSLLSRLGSEARLYRAMFL
                     EEIGKDYVRTARAKGLSEQVVLFRHVLKNALIPIITSAGAYLPYVFMGSLVFESFFGI
                     PGLGAYTIEAITGQDFAIVRSMVFLGSLLYVASYVLIDIAYTWADPRVRLT"
     gene            complement(2984..5275)
                     /locus_tag="VFV25_01270"
     CDS             complement(2984..5275)
                     /locus_tag="VFV25_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY1391973.1"
                     /translation="MLSPKLSRGGELGRRLLARLLTLGAAALATVLLAACNNSPYPSR
                     EAGQNILFNAFQERSPRYLDPTASYNNNETPITYQVYEPLYAYHYLKRPYALIPKVAE
                     EIAHPKYYDKDGHLLPDDVSGEQVAESVYEIHIKKGILYAPHPAFAKDEQGNYLYHKL
                     KPGELGDKHSPWDFPKQGTRELVADDFVYAFKRHATPRIEAPIYGIFSEYIVGLKDYG
                     KLIRAEDRKLLGDIPASSEDKPFLDFRKFPLEGVTAPDPYTLRMRIKGKYPQWKYWLA
                     MTFSAPVPWEADKFYSQPGMAAAGLRLTRWPVGTGPYMLTEYVQDRSHTLKRNPNYRG
                     EPYPCEGEPGDKAAGLLEDCGKMMPFVDTIVSTIEKEKVPMKEKFRQGYLDVPEIERP
                     DWGPEFIADRDDSDDVKKQFEERGFQFPQMTDINNWYLGFNWLDPVVGKGDTPAQQLR
                     NRKLRQAISIAIDWEEGYGRIFRNKGGVAAHSPLPPGVFGSREDQPGFYDHVTHKLVD
                     GKVVRRSIDEAKQLLAEAGYPDGRDAKTGQPLVLNYDYQRVPTPEIKAELDWMVKQFA
                     KIDIQLEIRATDYNQFNEKVLKGKQQIFWWGWLADYPDAENFLFLLYGPNSQAKYEGQ
                     NTANYQNDDYDRMYRQLQLLEDGPEKQKLIDQMVAVVQQDAPWAWGYFPYSAIATQHW
                     VHNGKPTIIVRDMAKYYRLDPKERVARLAAWNKPVWWPLLALVAIALGLVWIARRSYR
                     AREQAVGRAPAAVATQEVETASA"
     gene            complement(5588..6583)
                     /locus_tag="VFV25_01275"
     CDS             complement(5588..6583)
                     /locus_tag="VFV25_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEY1391974.1"
                     /translation="MTKKKKTEPEAQPPRLILFSPAGVVAQPVAVRRAAKRLAAHGYA
                     VSIDEAALVRQQRFAGDDDTRLAALHRIAKEGAQGRCSIAMATRGGYGLTRLLDRIDW
                     KLMAKSVAGGLRWVGYSDMTALQLAALTHGAGGDERAPHGHGVQGDWWAGPLAIDDFG
                     RTQEQGGVDDVTEACFLEAMHGELEAVGFRTDNGFDGLDVSGRLWGGNLCVLASLLGT
                     PHFPKPKQVRGGILFLEDVNEHPYRVERSLLQLHQAGVLDAQKAVLLGAFTDWRKSPL
                     DRGYTLKTAIAHLRSVTTTPILTGLPLGHIRTHVSLPHGRRATLAVQGRDAFLGW"
     gene            complement(6561..7190)
                     /gene="tadA"
                     /locus_tag="VFV25_01280"
     CDS             complement(6561..7190)
                     /gene="tadA"
                     /locus_tag="VFV25_01280"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEY1391975.1"
                     /translation="MSEASPVSPADEYAMRIALDQAQNAWLVGEVPVGAVIMRHTPDG
                     PQVIATGYNRPITTHDPTAHAEIVALRHAAQLLENYRLPECELYVTLEPCAMCAMALM
                     HARFKRVVFGAPDPKTGAAGSVLDLFASDKLNHHTEVVGGVLAEACGQLLRDFFAERR
                     AQQRAEREAGEPAASESADEAIPVGQAAEVEATHFDTIKDKKNDEEKEN"
BASE COUNT         1211 a         2310 c         2505 g         1241 t
ORIGIN      
        1 ccattcgatc gccttggcgc gcgccgcggg gccgcgcagc gaggtgtggg tctcgatcgc
       61 ctcgacgatc tggtcgccca cgcgcatcac cggattcagg ctggtggccg gctcctggaa
      121 gatcatgccg atgcgcccgc cccgcacatc gcgcatgcgc gcctcgggca gggccagcac
      181 gtcctcgcct tccagcgcca ggcggccgtc ggtgatgcgg ccgttctcgg gcagcaggcg
      241 catcagcgcc agcgcggtca tgctcttgcc gcagccggat tcgccgacca gcgcgaaggt
      301 ttcgccgcgc tggatgctca agcgcatgcc gtcgatggcc ttcacgaggc cgaagtcggc
      361 atcgagggcg accttgagat cgtcaatctg caacatggaa ctcgctcact tcacttgatc
      421 gccgcaggct tgggcatcgc agccaggcgg ggtcggaagg cacgcgagcg cgggtcgaac
      481 gcatcgcgca cgccgtcggc gaaaaggttg gcggccagca ccagcgtcac catgaagaag
      541 aaggcggcgg cgaaggacca ccacacgacg ggatcgcgcg acatctcgct gcgcgccagg
      601 ttgatcatgc cgccgaagct gctcatcgat ggatcgacac ccacgccgac ataggtcagc
      661 accgcctcgt agaggatgag gtcggagaag ctcagcaccg tcgtgatcag caccaggtgc
      721 accaggttgg ggaagacatg tcgcgccatg atgcgcgcgt gactcacgcc gaaggcggtg
      781 gcggcctgca cgtattcgag ttcgcgcagc ttgagcgtct cggcgcgcac caggcggcac
      841 agcgtggccc agccggtgag gccaaggatc acgcacaaca ggaagatgcg caggtccgag
      901 cgctcggcgc cggtctcgaa caggttcggg ttcttgtcca tgaagacctg caccatcagc
      961 acgcaggccg cgatcagcag cacattgggc accgaggaca gcgtcgtata gaggtactgg
     1021 atcagctcgt cgacccagcc cttgaaatag ccggccagga tgcccagcac gatcgcgaag
     1081 ggcagcgtcg ccagcgtcgc cagcgtgccg atcacgaagg cagtgcgcac gctcttcagt
     1141 gccagcagca gcacgtcgtt gccggtgcgg tcggtgccga agacgtggta gtggttcatc
     1201 agcgcggcaa tgggcccgca cagcaggcag ccgatgacga tggtgaggaa ggcggcgcgc
     1261 cacggcaggt cggtacggtt gccgaggatg gccgcaaccg cgtcgctcca gccgcgcttg
     1321 tggtggcgcg cgtagaagcc ggccgctgcc gcgccgacca ggccggcaaa cagcaggccg
     1381 ccgagcaggc cggtcacggc gcgcgagacg aggtcgcccg cccattcctg cgccggatcc
     1441 ttcaggtgcg cgccgccgaa tttgaggcgg gggtagtcgc gtacgctctg gccgtcgact
     1501 tcgaccgtgt ccttgttgaa gccggcgtag gcgagcgggc gcgaataggt cgtctcgcgc
     1561 atcgcgatct ggcgcgccag cgcgacatcg agcagcgact cggtgcgcgt gtcgtagaac
     1621 acgcgggtat cgccgttgga cgacggcggc agcgcgcgcc tgaagtgcac gctgtccagc
     1681 agcgtcacgc cgcagaacag cagcagcacc agggccgagc agagggccgc ggggtcgcgc
     1741 agcaccttca gccagttggc gcgcaggttg ggccgcttcg cgacgcgcca ggcgtagaag
     1801 gcggtgacgg cgaccaggat gtagatgacc acatcggtcc acagcaggac gaacttggga
     1861 ctcatgaccg caccccgccc ggcgagtacg ccgtgcaccc ctccgggagg ggacgggcct
     1921 tgcttgggag cggcccggcg cggcggcccg tggaaagaca ggttgacact ggatgtctca
     1981 tagcgcttgg gggctcaggt caggcgcacc cggggatcgg cccaggtgta ggcgatgtcg
     2041 atcagcacat agctcgccac gtagagcagc gagccgagga agaccatgga gcgcacgatg
     2101 gcgaagtcct ggcccgtgat ggcttcgatg gtgtaggcgc cgagccccgg aatgccgaag
     2161 aagctctcga acaccaggct gcccatgaag acatagggca gataggcgcc ggcgctggtg
     2221 atgatgggga tcagcgcgtt cttcagcacg tggcggaaca gcacgacctg ctccgacagg
     2281 cccttggcgc gcgcggtgcg cacatagtcc ttgccgatct cctccaggaa catcgcacga
     2341 tacagccgcg cctccgagcc caggcgcgac agcagcgaca gcgcgatcgg cagcaccagg
     2401 aacttggcgg catccagccc gccggcatag cccgagatcg gcgccagcct gaacacccgc
     2461 gagaacacga actggccgac gatgatgtag aacaggctgg agatcgacag catcagcacg
     2521 cacagcacca cgccccagaa gtcgatgcgc gagtgccgga agaagaccag catcagcgag
     2581 aaggccacgc tgatgatgac ctgcaggatg aacagcggca gcgccagctg caggctcacg
     2641 cccatgcggg tcttgatctc gtagccgatg tccaccgcgt tcttgctgtc ggcgcgaccg
     2701 aagtcgagct tgaacagcga gaccgagcgg tcccagacga tggtgtcggt gagcttggcc
     2761 gcgccgcttt ccttggcgct ccagtacagc ggcttgtcgt agccgcgctc ggccttccac
     2821 ttttcgatct gctcctgcgt gacgcgcttg ccgccgatgt tcaggcgcgc catgtcgtct
     2881 ggcgtgttga ccgtgaagaa caggaagaag gtgaagagat tgacgccgat gaggatcagc
     2941 acgccgtaac cgatgcggcg aatgatgtag ttcagcatga ggttcaggcg ctggccgttt
     3001 cgacttcttg tgtggcgacc gccgcgggag cccggcccac cgcctgctcg cgggcgcgat
     3061 agctgcggcg cgcgatccag accaggccca gggcgatggc aaccagcgcc agcagcggcc
     3121 accacaccgg cttgttccag gccgccagcc tggcgacgcg ctccttggga tcgaggcggt
     3181 agtacttcgc catgtcgcgc acgatgatgg tgggcttgcc gttgtgcacc cagtgctggg
     3241 tggcgatggc cgaatagggg aagtagcccc aggcccaggg cgcgtcctgc tgcaccacgg
     3301 ccaccatctg gtcgatcagc ttctgcttct cgggcccgtc ctcgaggagc tggagctgac
     3361 ggtacatgcg gtcgtagtcg tcgttctggt agttggccgt gttctggccc tcgtatttgg
     3421 cctgggagtt ggggccatac agcaggaaga ggaagttctc ggcatccgga tagtcggcca
     3481 gccagcccca ccagaagatc tgctgcttgc ccttgagcac cttctcgttg aactggttgt
     3541 agtcggtggc gcggatctcg agctggatgt cgatcttggc gaactgcttg accatccagt
     3601 ccagctcggc cttgatctcg ggcgtgggca cgcgctggta gtcgtagttc agcaccagcg
     3661 gctgaccggt cttggcgtcg cggccgtcgg ggtagccggc ctctgccagc agctgcttgg
     3721 cctcgtcgat ggagcggcgc accactttgc cgtccaccag cttgtgcgtg acgtggtcgt
     3781 agaagccggg ctggtcttcg cgcgagccga agacgcccgg cggcagcggg ctgtgcgcgg
     3841 ccacgccgcc cttgttgcgg aaaatgcggc catagccctc ttcccagtcg atcgcgatcg
     3901 agatggcctg gcgcaacttg cggttgcgta gctgctgcgc tggcgtgtcg cccttgccga
     3961 ccaccgggtc cagccagttg aagccgaggt accagttgtt gatgtcggtc atctgcggga
     4021 actggaagcc gcgctcctcg aactgcttct tcacgtcgtc ggagtcatcg cggtcggcga
     4081 tgaattcggg gccccagtcg gggcgctcga tttccggcac gtccagatag ccctggcgga
     4141 atttctcctt catcggcacc ttctccttct cgatggtgga gacgatggtg tcgacgaagg
     4201 gcatcatctt gccgcagtcc tcgaggaggc cggccgcctt gtcgcccggc tcgccctcgc
     4261 agggataggg ctcgccgcga tagttggggt tgcgcttgag cgtgtggctg cggtcctgca
     4321 catactcggt cagcatgtag ggcccggtgc ccaccggcca gcgcgtcagg cgcaggccgg
     4381 cggcggccat gccgggctgg gaatagaact tgtcggcttc ccagggcacg ggggcggaga
     4441 aggtcatcgc cagccagtat ttccactgcg ggtacttgcc cttgatgcgc atgcgcagcg
     4501 tgtaggggtc gggcgcggtg acgccttcca gcgggaattt gcggaaatcc aggaagggct
     4561 tgtcctcgct ggaggccggg atgtcgccga gcagcttcct gtcctcggcg cggatcagct
     4621 tgccgtagtc cttcaggccg acgatgtact cggagaagat gccgtagatc ggcgcctcaa
     4681 tgcgcggcgt cgcatggcgc ttgaaggcgt agacgaagtc gtcggccacc agctcgcgcg
     4741 tgccctgctt ggggaagtcc cagggcgaat gcttgtcgcc cagctcgccg ggcttgagct
     4801 tgtgatagag gtagttgccc tgctcgtcct tggcgaaggc gggatgcggc gcgtagagga
     4861 tgcccttctt gatgtggatc tcgtagacgc tttcggcgac ctgttcgccg gacacgtcgt
     4921 cgggcagcag atggccgtcc ttgtcgtagt acttggggtg ggcgatctcc tcggccacct
     4981 tggggatcag cgcatagggc cgcttgaggt agtgataggc gtacagcggc tcgtagacct
     5041 ggtaggtgat cggcgtctcg ttgttgttgt aggacgccgt cgggtcgagg tagcgcggcg
     5101 agcgctcctg gaaggcattg aacaggatgt tctggcctgc ctcgcggctg ggataggggc
     5161 tgttgttgca ggccgccagc agcacggtag ccagcgccgc ggcgccaagg gtcaacaggc
     5221 gagcgagaag gcggcggccc agctcgcccc cacgactgag cttggggcta agcatgcggc
     5281 ccgattccat gagtcttgta cacatcgctg aagggaagcc gcatgcggat caacgcatga
     5341 gcctcaaaaa aacaagcgcc tcgcgcgtac cgggcttttg gcccgaccga tgcaaggcat
     5401 accaatgata cttcctgaca gatcgtttac cgctcagggt ttcaccggcc gggccgtgcg
     5461 gacggcccgc gtgccaaggg tgagttgcgc ggcgccggag agatctcaga aaattgcctg
     5521 tctagcggtg tatttttacg gtaatcaact accaaatatg tagcatccgc aaacaccaaa
     5581 ctgatcgcta ccagccgagg aaggcgtccc gcccctgcac tgccagcgtt gcgcgccggc
     5641 cgtgaggcag gctcacatgg gtgcggatat ggcccagcgg caggccggtg aggatgggcg
     5701 tcgtggtgac cgagcgcagg tgtgcgatcg ccgtcttcag cgtgtagcca cgatccagcg
     5761 gcgacttgcg ccagtcggtg aaggcgccca gcagaaccgc tttttgcgca tccagcacgc
     5821 ccgcctggtg cagctgcagc aggctgcgct cgacgcgata gggatgctcg ttgacgtcct
     5881 cgaggaagag gatgccgccg cgcacctgct tgggtttggg gaaatgcggc gtgcccagca
     5941 gcgatgccag cacgcagaga ttgccgcccc acaggcgccc gctgacatcc agcccgtcga
     6001 agccgttgtc ggtgcgaaag cccacggctt ccagctcgcc gtgcatcgct tcgaggaagc
     6061 aggcttcggt gacgtcgtcg acaccgccct gctcctgcgt gcggccgaaa tcgtcgatcg
     6121 ccagcggccc ggcccaccag tcgccctgga cgccatggcc gtgcggcgcg cgctcgtcgc
     6181 cgcctgcgcc atgcgtcagc gcggcgagct gcagcgccgt catgtcgctg tagccgaccc
     6241 agcgcaggcc gccggccacg ctcttggcca tcagcttcca gtcgatgcgg tcgagcaggc
     6301 gcgtcagccc atagccgccg cgcgtggcca tcgcgatcga gcagcggcct tgcgcccctt
     6361 ccttcgcgat gcggtgcagc gcggccaggc gcgtgtcgtc gtcgccggcg aaacgctgct
     6421 ggcgcaccag ggccgcctcg tcgatcgaaa cggcatagcc atgcgctgcc aggcgcttgg
     6481 cagcgcggcg caccgcgaca ggctgcgcca ccacgccggc cggcgagaac aggatcagac
     6541 gcggcggctg cgcttcgggt tcagttttct ttttcttcgt cattcttctt gtccttgatg
     6601 gtgtcgaaat gcgttgcctc aacctcggcg gcctgcccca cgggaatcgc ttcgtcagcg
     6661 gattcggaag ctgcgggctc gccggcttcg cgctcggcgc gctgttgggc acggcgctcc
     6721 gcgaagaaat cgcgcagcag ctggccgcag gcctcggcca gcacgccgcc gacgacttcg
     6781 gtgtgatggt tgagcttgtc gctggcgaag aggtcgagca ccgagcctgc ggcgccggtc
     6841 ttgggatcgg gggcgccgaa gaccacgcgc ttgaagcgcg catgcatcag cgccatcgca
     6901 cacatcgcgc agggctccag cgtcacatag agctcgcatt cgggcaggcg gtagttctcc
     6961 agcagctggg ccgcgtggcg cagcgcgacg atctcggcat gggccgtcgg atcgtgcgtc
     7021 gtgatcggcc ggttgtagcc ggtggcgatc acctgggggc cgtctggcgt gtgccgcatg
     7081 atgaccgcgc cgaccggcac ctcgccgacc agccaggcgt tctgcgcctg gtcgagcgcg
     7141 atgcgcatgg catattcgtc ggccgggctg actgggctcg cttcgctcat ggcgcggatg
     7201 cgttcattga tcggcgtccg aggcggcgcg ctgggcgcct tgctgcaagg cgcgctgcat
     7261 ctcctgc
//
LOCUS       DASUCY010000062         2457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_997296, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000062 DASUCY010000000
VERSION     DASUCY010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997296"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1094)
                     /locus_tag="VFV25_01285"
     CDS             complement(<1..1094)
                     /locus_tag="VFV25_01285"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEY1391976.1"
                     /translation="MKTFLRRLSIRHRLYACAALFAVLTLGLGGWSHLSNRSSLAALE
                     QVLDEQQQTASRAGTLRSALGRLHEDEMSMMLNASNTLEVDRYKGQWLQELKTLRQGF
                     ADIAANGDAGLVEQARAGTAALAEYEQIITPIAQQLSGATMDVSAASAYAAKAAPNLA
                     AASKGVDALAQHAQEGLEATRSAVRRSGGVQAALAGALSVAAVALVALVMWLTLHSIC
                     EPIEQAKALAARIADGDLSSSLHVEGHDETAELLTSLLQMQSALRATVAEVRSSTDSI
                     TTASTEIASGNQDLSSRTEQAASSLQQTASSMEQLTGTVKQTADSARTANQLASSASA
                     SAAKGGAVVAQVVSTMDDINTSSKKIADIIG"
     gene            complement(1140..1619)
                     /locus_tag="VFV25_01290"
     CDS             complement(1140..1619)
                     /locus_tag="VFV25_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEY1391977.1"
                     /translation="MGMMEALEVKAPAAVREYLTFRLGNEEYGIDILKVQEIRGYEAP
                     TRIANAPAFLKGVVNLRGTIVPIVDLRLRFRCDSAEYNGFTVSIILHLGSRTIGAVVD
                     SVSDVLDIPAEAIKPAPEMKSAVDAGYILGLAQLGERMVILLDIEGMLLSEDMGLVA"
     gene            complement(1638..>2457)
                     /locus_tag="VFV25_01295"
     CDS             complement(1638..>2457)
                     /locus_tag="VFV25_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY1391978.1"
                     /translation="LKEMNESLVKIVGDVRTSTDTIATASGQIATGNQDLSSRTEEQA
                     SSLEQTAASMEELTSTVKQNADNARQANQLAVSASEVAVKGGSVVSQVVDTMASINAS
                     SKKIVDIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAARSAEAAK
                     EIKSLIGASVERVASGSQLVESAGRTMDEIVASVQRVTDIVSEISAASREQAGGIAEV
                     GQAVAQMDNATQQNAALVEQGAAAAASLREQAERLSQAVGVFRIAAGPALAAGY"
BASE COUNT          336 a          835 c          831 g          455 t
ORIGIN      
        1 ccgatgatgt cggcgatctt cttggagctg gtgttgatgt cgtccatcgt gctgaccacc
       61 tgggccacga cggcgccgcc cttggcggcc gaggccgagg ccgaggaggc cagctggttg
      121 gcggtgcgcg cggagtcggc cgtctgcttc accgtgccgg tgagctgctc catggacgag
      181 gcggtctgct gcaggctgga cgccgcctgc tcggtgcgcg agctcaggtc ctggttgccg
      241 ctggcgatct cggtgctggc cgtggtgatg ctgtcggtgg acgagcgaac ctcggccaca
      301 gtggcccgca gcgcggattg catctgcagc agcgaggtca gcagctcggc cgtctcgtcg
      361 tggccttcga catgcaatga gctggagagg tcgccgtcgg cgatgcgcgc ggccagcgcc
      421 ttggcctgct cgatgggctc gcagatggag tgcagcgtca gccacatcac cagggcgacc
      481 agcgcaacgg ccgccacgct gagcgcgccc gccagggcgg cctgcacgcc gccgctgcgc
      541 ctcacggccg agcgcgtggc ctccaggcct tcctgtgcgt gctgcgccag cgcatcgacc
      601 cccttggacg cggcggccag attgggcgcc gccttggccg cataggccga ggcagcgctg
      661 acatccatcg tggcgccgga cagctgctgc gcgatcggcg tgatgatctg ttcgtattcg
      721 gccagcgccg cggtgcccgc cctggcctgt tcgaccagcc cggcatcgcc gttggccgcg
      781 atgtcggcga agccttgcct cagagtcttc agctcctgca gccattggcc cttgtagcgg
      841 tcgacctcca gggtgttcga ggcgttgagc atcatgctca tctcgtcctc gtgcagccgg
      901 cccagcgccg agcgcagcgt gcccgcccgg ctggccgtct gctgctgttc gtcgagcacc
      961 tgttccagcg ccgccagcga actgcggttc gagagatggc tccagccacc cagccccagg
     1021 gtcaggacgg caaacagggc cgcgcaggca tacaggcggt ggcgtatcga caagcgcctc
     1081 aggaaggtct tcatggctcg gactccgttt gcgtgagagt gaattggcca cctaggccgt
     1141 caggcgacga ggcccatgtc ctcgctcagc agcatcccct cgatgtccag caggatcacc
     1201 atgcgctcgc cgagctgggc gaggccgagg atgtagccgg cgtcgacggc ggacttcatc
     1261 tcgggcgcgg gcttgatggc ctcggctggg atgtccagca cgtcgctgac cgagtcgacc
     1321 accgcgccta tcgtgcggct gccgaggtgc aggatgatgg agacggtgaa gccgttgtat
     1381 tcggcgctgt cgcagcgaaa gcgcaggcgc aggtcgacga tgggcacgat ggtgccgcgc
     1441 aggttgacca cgcctttcag gaaggccggc gcgttggcga tgcgcgtggg cgcctcgtag
     1501 ccgcggattt cctgcacctt gaggatgtcg atgccgtatt cctcattgcc gaggcggaag
     1561 gtgaggtatt cgcgtaccgc ggccggcgcc ttcacttcca gtgcttccat catgcccatg
     1621 tcggctgctc ctgtttttca atagcccgcg gccagggccg ggcccgccgc gatcctgaag
     1681 acgccgacgg cctggctcag gcgctcggcc tgttcgcgca ggctggcagc ggcggcggcg
     1741 ccttgctcca ccagcgcggc gttttgctgc gtggcgttat ccatctgcgc aacggcctgg
     1801 cccacctcgg cgatgccgcc cgcctgctcg cgcgaagcgg ccgagatctc ggaaacgatg
     1861 tcggtcacgc gctgcacgct ggccacgatc tcgtccatcg tccgacccgc gctttcgacc
     1921 agctgcgagc ccgatgccac gcgctcgacc gaggcgccga tcaggctctt gatctccttg
     1981 gcggcttcgg cactgcgtgc cgcaagcgaa cgcacctcgc tggcgacgac cgcgaagccg
     2041 cggccctgtt cgccggcgcg tgccgcctcg accgcggcgt tcagcgcgag gatgttggtc
     2101 tggaaggcga tgccgtcgat gacgccgatg atgtccacga tcttcttgga cgatgcgttg
     2161 atggatgcca tcgtgtccac cacctgactg accacgctgc cgcccttgac tgccacttcg
     2221 gaggccgaga cggccagttg attggcttgg cgtgcgttgt ccgcgttctg cttgaccgtg
     2281 ctggtcaact cctccatcga cgcagcggtc tgctccagcg aactcgcctg ctcttcggtg
     2341 cgcgaggaca agtcctggtt gcccgtcgcg atctggcccg acgccgtcgc aatcgtgtcc
     2401 gtgctcgtac gcacgtcgcc gacgatcttc accaggcttt cgttcatctc cttcagg
//
LOCUS       DASUCY010000063        11591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_210060, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000063 DASUCY010000000
VERSION     DASUCY010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 11591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11591
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210060"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VFV25_01300"
     CDS             complement(<1..402)
                     /locus_tag="VFV25_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEY1391979.1"
                     /translation="METPRDASMSANEVRVLRRFRIVFNAIKAHFQQVEKQAGVGGAQ
                     LWALSVIRQRPGIGVNELARAMDVHQSTASNLVKTLAERNLIVAAKEGSDRRAVQLRL
                     LASGAEVLDRAPGPCTGVLANALAALDADTLA"
     gene            635..901
                     /locus_tag="VFV25_01305"
     CDS             635..901
                     /locus_tag="VFV25_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1391980.1"
                     /translation="MAKDEVKTQIKASGLRYKSKASGSPFAATGAFQAGTMSCFLCGK
                     HRPRSALMTKRLLGKPQQVCSPSCKEAEAAANSASRSATTTTTA"
     gene            1164..2843
                     /locus_tag="VFV25_01310"
                     /pseudo
     CDS             1164..2843
                     /locus_tag="VFV25_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
     gene            complement(2867..3814)
                     /locus_tag="VFV25_01315"
     CDS             complement(2867..3814)
                     /locus_tag="VFV25_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEY1391981.1"
                     /translation="MMLFKAALPAGAMALLCSLAWAAPFAYVPNEGSGSLSVIDTASD
                     AVVATIAAGDKPRGTVVSLDGRTAYVSDQPNNRLVMIDLAQRRQSGAIPLGESPEGVG
                     ISPDGRWVAAAVEESNDVAFVDTRTNQKAFVVTVRGKNPEHAVFAPDGKHVFVSAEEG
                     EAVDVIDLEARKQVAQVPVGARPRGIGFSPDGRRAYVAAENANEVYVIDAADFKVLAK
                     IKAGTRSNGIAVHPDGKRVYVSNGGDASVSVIDTASNAVTATVAVGKRPWNMALTPDG
                     KKLYVACGRSGAVSVIDTERNVKLGDVAVGKLPWGVAIR"
     gene            complement(3832..4380)
                     /locus_tag="VFV25_01320"
     CDS             complement(3832..4380)
                     /locus_tag="VFV25_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEY1391982.1"
                     /translation="MQETRYSRVAMAFHWLIGLALLGETAFGFLLDEIAPRNTPARAE
                     VINLHKFIGIALGLLVLLRLLWRLGHRAPAWPPSIKQWQRSAAIAVHRALYALMIVVP
                     LSGYVASNFSKHGLKLYGLVLRPWGPDLPAVYAFFNTLHDVASYALVALIAGHVAMAL
                     KHLLIDRDGVFSRMWPWPEAES"
     gene            complement(4403..6556)
                     /locus_tag="VFV25_01325"
     CDS             complement(4403..6556)
                     /locus_tag="VFV25_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY1391983.1"
                     /translation="MDCDTLAQLPLSPGLAALLALLLVPLSAAAQSDAGTASTLPQVV
                     ITATRVETSPFDVPASIDRIGDDEIRDGRARINISESLGGVPGLLARDRQNYAQDVQI
                     SVRGFGARASFGIRGVRLYVDGIPATFPDGQGQISNVDLGSAASIEVLRGPFSALYGN
                     SSGGVIQVFTQEGAGPRKLELSAAGGSDRVMRAAATVSGADGPIGYLISASHFQTDGY
                     REHSAAERYIGNAKLSWHPDDSSKLTLVLNSVALPRAQDPLGLTRAQFEADPRSVDPS
                     ATQFNTRKSIDQTQGGLIYERRLDGVNSLRAMVYTGTRNTEQFQAIPVATQANPLNPG
                     GVIALGSDYSGTDLRWTARTSLAGRAFTLVAGLAYDRLGQLRRGYQNFIGKSLGVAGA
                     LRRDEDDGVSNLDQYLQASWQPAPAWTLNAGLRHSRVRFESQDHYIVGPNGDDSGAVQ
                     YGANLPAFGVLYAFNSGLHAYATAGRGFETPTFNELAYRPSGAPGLNFALQPARSLST
                     EIGLKARWAEAGEATAAVFETHTDNEIVSQTNQGGRSTYQNVGATKRSGIELSWANRF
                     FGNLRAHAAYTLLDAQYRDGFTTCATTPCAVPNQLIPAGNRIPGIARSSFFASLGWTP
                     VLGWRAGIEGRYLSRVYVNDANTDAAPSYAVASASLGYVSDLGLWHLHGFVRGDNLFA
                     RRYAGSVIVNESNGRYFEPAPGRTWLAGLSGTLSF"
     gene            complement(6608..7231)
                     /locus_tag="VFV25_01330"
     CDS             complement(6608..7231)
                     /locus_tag="VFV25_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018314108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEY1391984.1"
                     /translation="MSLLCLAVPVRAQSTDLPNPSAQLLAAARRADSVQVERALAQGA
                     APNARNRLGKTALLLASEQGAAALAQRLIEAGADVNLASLEGVTPLMAASYGGHAALA
                     KRLLDAGAQAETLDRMKKPAIVYAAAQGHAEVLGLLIAAGVDVNAGYEHGLTPLMWAA
                     GQGQAEAVKLLMARGAQAELRDDRGLTAAEIARRAGHAEIPALIGGR"
     gene            7481..9226
                     /locus_tag="VFV25_01335"
     CDS             7481..9226
                     /locus_tag="VFV25_01335"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018438890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HEY1391985.1"
                     /translation="MRHIDSTPCPWHRLRSCILAATVGLAATAAFAQAEISGTGAATG
                     SPVPKAVSVPQSRLDGATGDRSDFLHSNGNYAQTRYYPAAQINTANVGKLKPAFVFQT
                     EVLESMETAPIVVDGVMYLTTSYNHIYAIDAATGKEYWHYKHKQGPVTTFCCGPNNRG
                     VAIMGDRIYMGTLDAKLLALDAKTGKVLWAAQVADPEAGYSETMAPVAVNGKILIGTN
                     GGEYGVRGFVKAYDANDGKLLWTFYTIPDRGHEGVWAANDATGRDMHRDIAAEKKQLA
                     ERGGDFYKTLGGGVWMAPAVDKESNTVFFVVGNPSPDLYGAARPGDNLYTDSIVAVDL
                     NTGAYKWHYQYIAHDVWDLDAVSPVILTEAKDKAGKMRKVAIHGGKTGHIYVHDRTTG
                     ELIRYSEAMIPQENMWVLPTKDGARMLPGANGGVEWSPMAINPKLHLAYAANLHQPMT
                     YHVEEAQYPGGKLWLGGAFKVIPSEKQWGRLVAVNLDTGKLAWKYDTEQPLIGGVLAT
                     AGDLVFNGEGNGLFRAFDARNGKMLWQYQCGAGVNAPAVSYMVGGKQYVAVAAGGNTQ
                     LDFKRGNSVFVFALP"
     gene            9304..9888
                     /locus_tag="VFV25_01340"
     CDS             9304..9888
                     /locus_tag="VFV25_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013956666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY1391986.1"
                     /translation="MNPTRAWLGAVFSMSLALLAPQQTGAQASAPTKIETCTACHGPG
                     GNSTQPLIPILAGQTSRYLYLQLRDFQAGRRSNEMMTPMVAGLSPDEMRAMADWFAQQ
                     KPVPLSFKVDPEKARLGKLKADETLCTICHLGGFEGQNEIPRVAGQHHDYVVKQLSDF
                     KARRRTNDAGNMTSVSNTLSDADIENLAQYLSGL"
     gene            10158..>11591
                     /gene="adh"
                     /locus_tag="VFV25_01345"
     CDS             10158..>11591
                     /gene="adh"
                     /locus_tag="VFV25_01345"
                     /EC_number="1.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEY1391987.1"
                     /translation="MDMLEPGRFGFAVNFKKRYGNFINGQWVAPAKGEYFENITPVTG
                     KAFCEIPRSSAEDVERALDAAHAAKVAWGRSSPTERANILNRIADRMEQNLELLATAE
                     TWDNGKPIRETMAADLPLAIDHFRYFAGCIRAQEGTVAEIDHQTYAYHFHEPLGVVGQ
                     IIPWNFPLLMAVWKLAPALAAGNCVVLKPAEQTPVSILVLMELVGDLLPPGVLNVVNG
                     FGLEAGKPLASSNRIAKIAFTGETTTGRLIMQYASQNIIPVTLELGGKSPNIFFHDVC
                     AKDDGFLDKAIEGFVLFALNQGEVCTCPSRALVHESIYDQFMERALKRVAAIKQGNPL
                     DKSTMIGAQASGEQMEKIMSYLSLGKQEGAQCLIGGERNVLDGALKDGYYIKPTVFKG
                     HNKMRIFQEEIFGPVVSVTTFKDEDEALAIANDTLYGLGAGVWSRDMNTAFRMGRGIQ
                     AGRVWTNCYHAYPAHAAFGGYKQSGIGR"
BASE COUNT         1996 a         4004 c         3717 g         1874 t
ORIGIN      
        1 ggcgagcgta tcggcatcca gcgcggcgag cgcattggcc aggacgccgg tgcagggccc
       61 gggcgcgcgg tccagcacct cggcacccga ggccagcagc cgcagctgga ccgcgcggcg
      121 gtcgctgccc tccttggccg ccacgatcag attgcgctcg gccagcgtct tcaccagatt
      181 gctggcggtg gactggtgca cgtccatcgc acgcgccagc tcgttgacgc cgatccccgg
      241 cctttgcctg atcacgctga gcgcccacag ctgggcaccg ccgacaccgg cctgcttctc
      301 gacctgctgg aagtgggcct tgatcgcgtt gaagacgatc cggaagcgcc gcagcacccg
      361 cacctcgttg gccgacatgc ttgcgtcgcg cggggtttcc actgcgctct gatgccgtcg
      421 tttggcgggg ctggctggca tggtccgatg atagtggggc cccgcaacgt gccactcgac
      481 cgaaggggca aaacagctac cagaaacgcg agttttgtca agcgttacca tgtcgggtgg
      541 gagattcaca acatgactat ggattcgcaa gctgcaagga catttgaact aaagccagct
      601 gaccccaatt tcgatttttc gcaagagtaa gttcatggcc aaagacgaag ttaagaccca
      661 gatcaaagcc agcggcttgc gctacaagtc caaggcgagc ggctcgccct tcgctgccac
      721 gggggcattt caggccggaa cgatgtcgtg cttcttgtgc ggcaagcaca ggccgcgcag
      781 cgcgctgatg accaagcgcc tgctcggcaa gccacaacag gtgtgctcgc cttcctgcaa
      841 ggaagccgaa gccgcggcca attccgccag ccgcagcgcg accaccacca cgacggccta
      901 agccgtccaa ggtggttggc cgggaggccg atcacctgcg cctcggcgac ctgcatcgac
      961 gctgcgcgcc gatgcttgca aacgggcgtg agggcgcctc gaatccggtg cgcgtcactg
     1021 cctcatgcgc gcccgttcca tcactgccaa ctcccgcgtc tggctcctga cgcggcggcg
     1081 gcgcttcatc gcgcgtgcgc accattgcgg acctccggcc gcggctctcg ccgcctgccc
     1141 aagccaccct acccggaggt tccatgccgc tgcctgccca gttccaacgc tttcgcccgc
     1201 gcctgctcga tgcattggcg gcgggttatg accgccagcg cttctttgcc gacctcggag
     1261 ccggcatcac cgtcggcatc gtcgccctgc ccttggccat ggcctgcgcc atcgcctcgg
     1321 gcgtcaaacc cgagcagggc ctgttcacgg ccatcatcgc cggactgctg atctcgctgc
     1381 tcggcggctc gcaggtgcag atcggcgggc cggctggcgc cttcatcgtt attgtttgcg
     1441 gcatcgtcca acgctacggc ctggccaacc tgctgatcgc cacctccctg gccggcatcg
     1501 cgctgttcct gatgggcctg ttcaagctgg gcgcgctggt gcgctacctg ccggtcagca
     1561 tcgtgatcgg cttcaccaac ggtatcgcgg tgctgattgc gctctcgcag ctgcgcgacc
     1621 tgctgggcct gaagctcgac aagctgccgg cggacttctt cggccagctg catgcgctgg
     1681 gcacgcacat cgacagcttc aacccctatg ccttcgcctt gggcagcgtc tgcgtcgccg
     1741 ggctgctgct gtgggcacag ctcttcaagc ccgggtcgcc ggtgctgcag gcgcagctcg
     1801 acaagcgcgc aacccgtctc gcggcgcgcg tgcccggtcc catcatcgcg ctggtcagcc
     1861 tcagcgccgc agcggcctat tggcagctgc cggtcgagac ctgggctcgc gcttcggaga
     1921 gattccgcgc gcactgcccg ccttcaccct gcccgccttc aattgggcaa cggtgcagca
     1981 gctcttcatt ccgacgctga cgatcgccat gctgggcgcc gtggaatcgc tgctgcgcgc
     2041 gcgcgtggcc gacaacctga tcgagcacgc ccgccacgac cccaaccagg aattgatggc
     2101 acagggcgtc gccaacttct tcacgccgct tttcggcggc attccggccc ccggcaccat
     2161 cgcgcacggt caccaacgtc cgcgccggcg cggccacgcc gattgcgggg atcgtgcacg
     2221 cgctgacggt gcttgccatc gtgctgctgg ccgcgcctct ggcctcacgc gtgccgctgt
     2281 gcgtgctggc cggcatcctg ctcgtcgtgg cctggaacat gggcgactgg cacgagttcg
     2341 cgcgcctcag gcagttcaat gtcacctacc gcaccatcct ggtcggcacc ttcgtgctca
     2401 cggtggtgat cgacttgacg gtggctgtcg aggtcggcct ggtgctggcc tgcatctttt
     2461 tcatctggcg catgagcgag cagtttcgcg tcgaagcgct gtggcttccc gctgcaccac
     2521 ccggcgtaca ggtgctggag ctctacggcg cgctcttctt cggcgcaata ggcaaggtcg
     2581 agacgctgcc gcagaacctg cctgcaggca cgcacaccgt agtgctggac acccagcgcc
     2641 tggtgctgat ggacagctcc ggcctggacg cgctgctgtt gctgcatcgc cagctggagc
     2701 gccagaacat gcgcctgctg ctgtgcgggc tcaacgagca gccggcttcg ctgctgcgac
     2761 gcgcgggtgc cgatgcggta ctgggcgagg ccaatatcga tccggatctg ccgacggctt
     2821 tggcgcaact gcgccaggct tgaacggcag actcgatcgc tcggcctcag cgtatggcga
     2881 cgccccaagg cagcttgcca accgccacat cgcccagctt gacgttgcgc tcggtgtcga
     2941 tcaccgagac ggcgccagag cgcccgcagg ccacatagag cttcttgccg tcgggcgtca
     3001 gcgccatgtt ccaggggcgc ttgccgaccg cgaccgtggc cgtgacggca ttgctggccg
     3061 tgtcgatcac cgacacgctc gcatccccgc cgttggaaac atagacccgc ttgccgtccg
     3121 ggtgcaccgc gatgccgttg ctgcgcgtgc cggccttgat cttcgccagc accttgaagt
     3181 cggccgcatc gatcacatag acctcgttcg cgttctccgc cgctacatag gcgcgccggc
     3241 cgtccggcga gaagccgatg ccgcgcggcc gcgcgccgac tgggacctgc gccacctgct
     3301 tgcgtgcctc cagatcgatc acgtcgaccg cctcgccctc ctccgcgctg acgaacacat
     3361 gcttgccgtc gggcgcgaac acggcatgct cggggttctt gccgcgcacg gtgacgacaa
     3421 aggctttctg gttcgtgcgc gtgtcgacga aagcgacgtc gttgctttcc tcgacggcgg
     3481 cagccaccca gcggccgtcg ggtgaaatgc ccacgccctc gggcgattcg ccgagcggga
     3541 tcgcgccgga ctgccggcgt tgcgcgaggt cgatcatcac gaggcggttg ttgggctggt
     3601 cgctgacata ggccgtgcgg ccatccaggc tgaccaccgt cccgcgcggc ttgtcgcctg
     3661 ccgcgatggt cgcgacgacg gcatcgctcg cggtatcgat cacgctcagg ctgccggagc
     3721 cttcgttggg cacgtaggca aagggagcgg cccaggccag tgagcacagc aaggccatgg
     3781 caccggccgg cagcgcggct ttgaacaaca tcatgccaat tccctctctc attacgattc
     3841 ggcctcaggc cagggccaca tgcgcgagaa cacgccatcg cggtcgatca gcagatgctt
     3901 gagcgccatc gccacatgcc cggcgatcag ggccaccagg gcatacgatg cgacgtcgtg
     3961 cagcgtgttg aagaaggcgt acaccgccgg caggtcggga ccccagggcc gcagcaccag
     4021 gccatacagc ttcagcccat gcttgctgaa attggaagcc acatagcccg acagtggtac
     4081 cacgatcatc agggcataca gagcgcgatg gaccgcgatc gccgcgctgc gctgccactg
     4141 ctttatcgag ggtggccacg cgggcgcccg atgccccagg cgccacagca gccgcaagag
     4201 gaccaaaagg cctaacgcaa tgccaatgaa cttgtgcaga ttgatcacct cggcgcgcgc
     4261 cggcgtgttg cgcggtgcga tctcatcgag caggaagccg aatgcggtct cgcccaatag
     4321 ggcgaggcca atcaaccagt ggaacgccat ggcgacgcgg ctgtagcggg tttcttgcat
     4381 ggaggagcct gccccgtgtc gatcagaagc ttagcgtgcc cgacaggccg gccagccagg
     4441 tccgcccggg cgccggctcg aaatagcgcc cattgctctc gttgacgatg accgaacccg
     4501 catagcggcg cgcgaacagg ttgtcgccgc gcacgaagcc atgcagatgc cataggccga
     4561 gatccgagac atagcccagg ctcgcgctgg cgacggcata gctgggcgcc gcatcggtat
     4621 tggcgtcgtt gacatagacg cggctcaggt agcggccctc gatgccggcc cgccagccca
     4681 gcacgggagt ccagcccagc gaggcgaaga aagacgagcg cgcgatgcca gggatgcggt
     4741 tgccggccgg tatcagctgg ttcggtacgg cgcacggggt ggtggcgcag gtcgtgaagc
     4801 cgtcacggta ttgcgcgtcg agcaaggtgt aagcagcgtg ggcgcgcaga ttcccgaaga
     4861 agcggttcgc ccatgagagc tcgatgccgc tgcgcttcgt cgcgcccaca ttctggtagg
     4921 tcgagcgccc gccctgattg gtctgggaga cgatctcgtt gtcggtatgg gtctcgaaga
     4981 cggcagccgt cgcttcgccg gcctcggccc agcgtgcctt caggccgatc tcggtgctca
     5041 ggctgcgcgc cggctgcaac gcgaaattca gccccggggc accgctgggc cgataggcca
     5101 gctcgttgaa ggtcggcgtc tcgaagccgc gccccgccgt ggcgtaggcg tgcaggccgc
     5161 tgttgaaggc atacagcacc ccaaacgcag gcaggttcgc accgtattgc actgccccgc
     5221 tgtcgtcgcc gttggggcca acgatgtaat ggtcctggga ctcgaagcgc actctgctgt
     5281 ggcgcaggcc ggcattcaag gtccaagcgg gcgccggctg ccaggaggcc tgcaggtatt
     5341 ggtcgaggtt ggagacgccg tcgtcctcgt cgcgccgcag cgcgccggcc acgcctagcg
     5401 acttgccgat gaagttctgg tagccgcgcc tgagctggcc gagccggtca taggcgaggc
     5461 cggcaacgag cgtgaaagcc cgcccggcca gcgaggtgcg cgccgtccag cgcagatcgg
     5521 tgccgctgta gtcgctgccc aatgcgatca cgccgccggg gttgagggga ttggcctgcg
     5581 tcgcgaccgg gatggcctgg aactgctcgg tattgcgtgt gcccgtgtag accatggccc
     5641 gcagcgaatt gacgccatcg aggcgacgct cgtagatcaa gccgccctgg gtctggtcga
     5701 tgctcttgcg ggtgttgaac tgcgttgcgc tcggatcgac gctacgcgga tcggcttcga
     5761 actgggcccg cgtcagcccc agaggatcct gggccctcgg cagggccacg ctgttgagga
     5821 ccagggtcag cttgctggaa tcgtcggggt gccagctcag cttggcattg ccgatgtagc
     5881 gctcggccgc gctgtgctcg cgatagccgt cggtctggaa gtggctggca ctgatcaggt
     5941 agccgatcgg cccgtcggcg ccgctcaccg tggccgccgc gcgcatcacg cggtcgctgc
     6001 cgccagcggc gctgagttcg agcttgcgcg ggccggcccc ttcctgcgtg aagacctgga
     6061 tcactccacc cgaggaattg ccatacaagg ccgagaacgg gccgcgcagc acctcgatgc
     6121 ttgcggccga gcccaggtcc acgttcgaga tctggccctg gccatcggga aatgtggccg
     6181 gaatgccgtc aacgtagagc cgcacgccgc gtatgccgaa gctcgcgcgc gcgccgaagc
     6241 cgcgcaccga gatctgcacg tcctgcgcat agttctggcg atcgcgagcc aggaggccgg
     6301 gcacgccgcc caggctctcc gagatattga tccgggcccg gccatcgcgg atctcgtcgt
     6361 cgccgatgcg atcgatggag gcgggtacat cgaagggcga cgtctccacg cgcgtggccg
     6421 tgatcacgac ctgcggcagc gtgctcgccg tacccgcgtc ggattgcgcc gccgcagaca
     6481 aagggacaag caggagggcc agcaaggcgg ccaggcccgg ggaaagaggt agttgcgcca
     6541 aagtgtcaca gtccatattg ccgggccttg cacatgcaat tcgcctgcca atgaagcgcc
     6601 agcattgtca tcgaccaccg atcaaggccg gaatctccgc gtggccggcc cgccgcgcga
     6661 tctctgcggc cgtcaagccg cggtcgtcgc gcagctccgc ctgcgccccg cgcgccatca
     6721 gcagtttcac ggcctcggcc tgaccctgcc ccgccgccca catcagcggc gtgaggccgt
     6781 gctcatagcc ggcgttcaca tcgacgccgg ccgcgatcag caggcccagc acctccgcat
     6841 gcccctgggc tgcggcatag acgatggccg gcttcttcat gcgatccagc gtctcagctt
     6901 gggccccggc atcgagcaga cgcttggcga gggcggcatg cccgccgtag ctggcggcca
     6961 tcagcggcgt cacaccctcg agcgaggcga gattgacgtc ggcccccgcc tcgatcaagc
     7021 gctgcgccag cgccgccgcg ccctgctcgc tggccagcag caaggcggtc ttgcccagtc
     7081 ggttgcgcgc gttgggcgcg gcgccctgtg ccaacgcccg ctcgacctgg acgctgtcgg
     7141 cccggcgcgc tgccgccagg agctgcgcgc tcgggttggg caagtcagtc gattgcgcgc
     7201 gcaccggcac cgccagacag agcagcgaca tcaagaccac cgccgcgcca gacctgtacc
     7261 agctccccga atactgcccg cgcatcacca ccctactgaa aacgccgcca gagcttgcaa
     7321 ctatgcgttt tgtgactatc ctcgcaagcg aggaaacgcg cgacccgacc cacgcgcagc
     7381 ccagcagttc atcgagactg cctggagccg agccctccac gttgtcttct tccccgcgtc
     7441 ctgcaacgcg gcccgacgga caagcaacaa ggagactcgc atgcgtcata tcgactcgac
     7501 gccgtgcccg tggcaccggc ttcgctcttg catcctggcg gccacggtcg gcctggccgc
     7561 gaccgcggcc ttcgcccagg ccgagatttc cggcacggga gccgcgaccg gttcgccggt
     7621 gcccaaggcc gtctcggtgc cccagtcgcg gctggatggg gcgacgggcg atcgatccga
     7681 tttcctgcac tccaacggca actatgcgca gacgcgctac tacccggcag cgcagatcaa
     7741 cacggccaat gtcggcaagc tcaaacccgc cttcgtgttc cagaccgagg tgctggaatc
     7801 aatggagacg gcgcccatcg tggtcgacgg cgtgatgtac ctgaccacct cgtacaacca
     7861 catctacgcg atcgatgcgg ccaccggcaa ggagtactgg cattacaagc acaagcaggg
     7921 gccggtgacg accttctgct gcggccccaa caaccgcggc gtggcgatca tgggcgatcg
     7981 catctacatg ggcacgctgg acgccaagct gctggcgctg gacgccaaga ccggcaaggt
     8041 gctgtgggcg gcccaggtgg ccgatcccga agccggttat tcggaaacca tggccccggt
     8101 ggcggtgaac ggcaagatac tcatcggcac caacggcggc gaatacggcg tgcgcggctt
     8161 cgtgaaggcc tatgacgcga acgatggcaa gctgctgtgg accttctaca ccatccccga
     8221 tcgtggccac gagggcgtat gggccgccaa cgatgcgacc ggacgcgaca tgcatcgcga
     8281 catcgcggcc gagaagaagc agttggccga gcgcggcggc gacttctaca agaccctggg
     8341 cggtggcgtg tggatggcgc ccgccgtgga caaggagagc aacacggtct tcttcgtggt
     8401 cggcaacccc agccccgatc tctacggcgc cgcgcggccg ggcgacaacc tctacaccga
     8461 ctccatcgtc gcggtcgacc tgaacaccgg tgcctacaag tggcactacc agtacatcgc
     8521 ccacgacgtc tgggacctgg acgccgtctc gcccgtgatc ctgaccgagg cgaaggacaa
     8581 ggccggcaag atgcgcaagg tcgcgatcca cggcggcaag accggccaca tctacgtgca
     8641 cgaccgcacc accggcgagc tgatccgcta cagcgaggcg atgatcccgc aggagaacat
     8701 gtgggtgctg cccaccaagg atggcgcgcg catgctgccc ggcgcgaacg gcggcgtcga
     8761 atggagcccg atggcgatca atcccaagct gcacctggcc tatgcggcca atctacacca
     8821 gccgatgacc tatcacgtcg aggaggccca gtaccccggc ggcaagctgt ggctgggcgg
     8881 cgccttcaag gtcatcccca gcgagaagca atgggggcgc ctggtggcgg tgaacctcga
     8941 caccggcaag ctcgcctgga aatacgacac cgagcagccc ttgatcggcg gcgtgctcgc
     9001 caccgccggc gacctggtct tcaacggcga gggcaatggc ctgttccgcg ccttcgatgc
     9061 ccgcaacggc aagatgctgt ggcaatacca atgcggcgcg ggcgtgaacg caccggctgt
     9121 gtcctacatg gtcggcggca agcaatacgt agcagtggcc gcaggcggca atacccagct
     9181 cgacttcaag cgcggcaatt cggtcttcgt gtttgccttg ccctgagcgg gctggtcttg
     9241 acgcatgaca gggattcggg gccgccaccc cgagtccttg cctggaatag aagagatgcc
     9301 tgcatgaatc caactcgcgc gtggctgggc gctgtctttt caatgagcct ggctctcctc
     9361 gcgccgcaac agaccggtgc acaggcctcg gctccgacca agatcgagac ctgcacggcc
     9421 tgccacgggc cgggcggcaa ctccacccag cccttgattc ccatcctcgc cggccagacc
     9481 tcccgctatc tttatctgca gctgcgcgac ttccaggccg gccggcgcag caacgagatg
     9541 atgacgccca tggtcgccgg cctgagcccg gacgagatgc gcgccatggc cgactggttc
     9601 gcgcagcaga agcccgtgcc actgagcttc aaggtcgatc ctgagaaggc caggctcggc
     9661 aagctcaagg ccgacgagac actgtgcacg atatgccatc tcggcggctt cgaggggcag
     9721 aacgagatcc ctcgcgtggc cgggcagcac cacgactatg tcgtcaagca attgagcgac
     9781 ttcaaggccc gccgccgcac caacgacgcc ggcaacatga ccagcgtgag caacacgctg
     9841 agcgacgcgg acatcgagaa cctggcgcaa tacctctccg gcctgtgacc acccgcgggc
     9901 gtcgctcatc gagcccgttg cgcgacagcc gctgtaccga aacgcaacgc tgaagctccg
     9961 gcgatgtgca agcgcagcac atcgcccgct ctgcgcctcg gtatttgccc gcgctcagcc
    10021 gggctcggca gcggcatagg cgccgcgaac ccccatctgg cacggcgctc gcaatgaggc
    10081 gccccagcag ccgaacatcc gccccacccc gtgctgcatc aatggcggga agggcctata
    10141 tcttcaggag agaacacatg gacatgctcg aaccgggccg cttcggcttc gccgtcaact
    10201 tcaagaagcg ctacggcaac ttcatcaacg gccagtgggt cgcgccggcc aagggcgaat
    10261 acttcgagaa catcacgccc gtcaccggca aggccttctg cgagatcccg cgttcctccg
    10321 ccgaagacgt cgagcgtgcc ctcgatgcgg cgcatgccgc caaggtggcc tggggccgta
    10381 gctctcccac ggagcgcgcc aacatcctca accgcatcgc cgaccgcatg gagcagaacc
    10441 tcgagctgct cgcgaccgcc gagacctggg acaacggcaa gcccatccgc gagacgatgg
    10501 cggccgacct gccgctggcg atcgaccact tccgctattt cgccggctgc atccgcgcgc
    10561 aggaaggcac ggtcgccgag atcgaccacc agacctatgc ctatcacttc cacgagccgc
    10621 tgggcgtcgt gggccagatc atcccgtgga acttcccgct gctgatggcg gtatggaagc
    10681 tcgcgcctgc gctggccgcc ggcaactgcg tggtgctgaa gcccgccgag cagacgcctg
    10741 tgtccatcct ggtactgatg gagctggtcg gcgacctgct gccgcccggc gtgctcaacg
    10801 tggtcaacgg cttcggcctg gaagcgggca agccgctcgc gtcgagcaac cgcattgcca
    10861 agatcgcctt caccggcgag acgaccaccg gccggctcat catgcaatac gccagccaga
    10921 acatcatccc ggtgacgctg gagctgggcg gcaaatcgcc caacatcttc ttccacgatg
    10981 tctgcgccaa ggatgacggc ttcctggaca aggccatcga aggcttcgtg ctgttcgcgc
    11041 tcaatcaggg cgaggtctgc acctgcccct cgcgcgcgct ggtccacgag tcgatctatg
    11101 accagttcat ggagcgcgcg ctcaagcgcg tggccgcgat caagcagggc aacccgctgg
    11161 acaagagcac gatgatcggc gcgcaggcct ccggcgagca gatggagaag atcatgagct
    11221 acctcagcct cggcaagcag gaaggcgcgc aatgcctgat cggcggcgag cgcaacgtgc
    11281 tcgacggcgc cctgaaggac ggctactaca tcaagcccac cgtcttcaag ggccacaaca
    11341 agatgcgcat cttccaggaa gagatcttcg gcccggtggt gtcggtgacg accttcaagg
    11401 atgaagacga agcgctcgcc atcgccaatg acacgctcta cggcctgggt gccggcgtgt
    11461 ggagccgcga catgaacacc gccttccgca tgggccgcgg catccaggcc ggccgcgtgt
    11521 ggaccaactg ctaccacgcc tacccggcgc atgcagcctt cggcggctac aagcaatcgg
    11581 gcatcggtcg c
//
LOCUS       DASUCY010000064         2297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_611662, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000064 DASUCY010000000
VERSION     DASUCY010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611662"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VFV25_01350"
     CDS             complement(<1..248)
                     /locus_tag="VFV25_01350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEY1391988.1"
                     /translation="MRSDEAADVVTYGRREDVLSLAQGIAERQGLDPDWVTQTLAQAR
                     FVPNVVRLIMPPPAGTAKNWAAYRDRFIEPTRLRAGVE"
     gene            complement(380..>2297)
                     /locus_tag="VFV25_01355"
     CDS             complement(380..>2297)
                     /locus_tag="VFV25_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3488 and DUF4129 domain-containing
                     transglutaminase family protein"
                     /protein_id="HEY1391989.1"
                     /translation="ALGSAPLPGRWMPAVVLLVAAVLTLWSYRTLLGKEAGVTLIVVL
                     AALKTLELRARRDAFVVFFLGFFLVLTQFLYSQSLLTAASMLVAVWGLLTGLVLAHMP
                     VGQPALRQAAALAARSALLGAPLMALLFLLFPRVAPLWGVPQEALGRTGLSAEMSMGS
                     IAQLAVDDSVAMVLRFPDGQAAPQELLYFRGPVLGDFDGRSWRVLEPRFSGDPPLRTE
                     LRLIGKPLRYELTLEPQRLPLLPLLDVTPEAPALEGDADNLRATRRSDLNWVTNRPIV
                     DRLRLTAEAWLAFRHGPRQDEPGLRAYLALPADYNPRTLAWAAQLRSDPRYAQADAHT
                     LATAILQRIRTGGYSYTLTPGFYGDGDIRGKNSPYDAIDEFWLDRKAGFCEHFSAAFV
                     VVMRAMGVPARIVTGYQGAELNPYDGSYVVRQSFAHAWAEYWQPGAGWLRADPTAAVA
                     PDRIQRGLSLRPPRGLVAGAIANVSPALQEQLREFFEAVNNQWNRWVLNYSHGRQLDL
                     LKNLGFDSPNWQDLSLLLIGLLAGLALLGALWAWWDQRRMDPWLRAYGQVQRALAAAG
                     LEAPAHLPPRSLAQRLMSQRPDGAEIAGGLRELDTLRYGPQARDPHEAATLARKLARS
                     LLVRIRKLPRRLPG"
BASE COUNT          375 a          784 c          841 g          297 t
ORIGIN      
        1 aactcgacgc ccgcgcgcag gcgcgtcggc tctatgaagc gatcgcggta ggcggcccag
       61 ttcttggccg taccggccgg cggcggcatg ataaggcgca ccacattcgg cacgaagcgg
      121 gcctgggcca gggtctgcgt cacccagtcg ggatccaggc cctggcgctc ggcgatgccc
      181 tgggccaggc tcaggacatc ctcgcggcgg ccataggtga cgacatcggc agcttcatcg
      241 ctgcgcacgg ctgctggctt cttgggtgcg gcgagtgtgg gagtggggga cgcgagacaa
      301 aggcccagca gggcgagcgc gaagacgggg aggggcaaac gcatcggtac aacagcgaca
      361 aatggcaaca gcggaaagct cagccaggca gccttcgcgg tagcttcctg atccgcacca
      421 gcaggctgcg cgcgagcttg cgggccagtg tagcggcctc gtgcgggtcg cgggcctgtg
      481 ggccgtagcg aagtgtgtcc agctcgcgca ggccaccggc gatctcggcg ccgtcggggc
      541 gctggctcat cagccgttgc gccaggctgc gcggcggcag atgcgcaggc gcctctagtc
      601 ccgctgcggc cagggcgcgc tgcacctggc cgtaggcgcg cagccagggg tccatgcggc
      661 gctggtccca ccaggcccac agcgcgccca gcagggccag accggccagg aggccgatca
      721 gcagcagcga gaggtcctgc cagttcggcg agtcgaagcc caggttcttc agcagatcga
      781 gctggcggcc gtgcgaatag ttgagcaccc agcggttcca ttggttgttg accgcctcga
      841 agaactcgcg caactgctcc tgcagcgccg ggctcacgtt ggcgatggcg cccgccacca
      901 gcccgcgcgg cggcctcagg ctcaagcccc gctggatgcg atcgggggcg accgcggcgg
      961 tcggatcagc gcgtagccag ccggcaccgg gttgccagta ctcggcccag gcatgggcga
     1021 agctctggcg caccacatag ctgccgtcgt aggggttgag ctcggccccc tggtagccgg
     1081 tgacgatgcg tgccggcacg cccatggcgc gcatcacgac cacgaaggca gccgagaaat
     1141 gctcgcagaa gccggccttg cgatcgagcc agaactcgtc gatcgcgtca taggggctgt
     1201 tcttgccgcg gatgtcgccg tcgccgtaga acccgggcgt cagcgtatag ctatagccgc
     1261 ccgtgcggat gcgctgcagg atcgcggtgg ccagcgtgtg cgcatcggcc tgcgcatagc
     1321 gggggtcgct gcgcagctgc gcggcccagg ccagggtgcg cgggttgtag tcggccggca
     1381 gcgcaagata ggcgcgcagc cctggctcgt cctggcgcgg gccgtggcgg aaggcgagcc
     1441 aggcctcggc cgtgagccgc agcctgtcga cgatggggcg gttggtgacc cagttcaaat
     1501 cgctgcggcg cgtggcgcgc agattgtcgg cgtcgccttc cagcgccggg gcctcgggcg
     1561 tcacgtccag cagcggcaag aggggtaggc gttgcggctc cagcgtcagc tcgtagcgca
     1621 ggggcttgcc gatcaggcgc agctcggtgc gcagcggcgg atcgccgctg aagcgcggct
     1681 ccagcacgcg ccaggagcgg ccgtcgaagt cgcccagcac cgggccgcgg aaatacagca
     1741 gctcctgggg tgccgcctgg ccgtccggga agcgcagcac catggccacg ctgtcgtcga
     1801 ccgccagctg ggcgatcgag cccatgctca tttcggccga caggccggtg cgccccagcg
     1861 cctcctgcgg cactccccac agcggcgcca cccgtgggaa gagaaggaag agcagggcca
     1921 tcagcggcgc ccccagcagg gccgagcgtg ccgccagcgc cgccgcctgg cgcagcgccg
     1981 gctggccgac cggcatatgg gccagcacca gccccgtcag caagccccat acagccacca
     2041 gcatcgacgc cgcggtaagc agggactgcg aatacaggaa ctgcgtcagt accaggaaga
     2101 agccgaggaa gaagacgacg aaggcgtcgc gccgcgcgcg cagctccagc gtcttgagcg
     2161 cggcgagcac cacgatgagc gtcacccctg cctccttgcc gagcagggtg cgatagctcc
     2221 acagcgtcaa gactgccgcg accagcagca cgacggccgg catccagcgg ccgggcagcg
     2281 gcgcgctgcc gagcgcc
//
LOCUS       DASUCY010000065         2924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1543437, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000065 DASUCY010000000
VERSION     DASUCY010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1543437"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1518
                     /locus_tag="VFV25_01360"
     CDS             <1..1518
                     /locus_tag="VFV25_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY1391990.1"
                     /translation="HSAGRRGFGNADVILNLMPDRFANGNPANDDVPGFADKANRADN
                     DAGRHGGDIQGIVDHLDHIAAMGYTMLWPTPLTESNQPHYSYHGYAATDTYRIDPRYG
                     SNEDYRRMVAAARERGIGVIQDIVLNHIGSNHWWMRDLPTQDWVSGGSHYVPTGHART
                     AISDPYAAEVDRESFIAGWFGPEMPDMNQRNPLLATYQIQNTIWWIEYAGLAGIRADT
                     YGYSDKHFLAQWSGRVMLEYPRLNIVGEEWSNNPVVTSYWLRGKKNADGYVSNLPSVM
                     DFPLQATLLRALVAPEGLHSGLVDVYEAMVNDRLYPDPMNLVLFEGNHDLPRLYSMVD
                     EDLALWKMALAFVLTAPRIPQIYVGTEILMTSPKVRDDGAFRHDFPGGWAGDKVNAFT
                     GEGLSDRQKEAQAWLKRVLNWRKTQPAVHRGKFMHYQPQDGMYVYFRYDAKGLVMVVL
                     NKNAGDATLATERFRQVLPQGASGTDVLTGRHYELGDTLVVPARSVLILDLKRPG"
     gene            complement(1532..2422)
                     /locus_tag="VFV25_01365"
     CDS             complement(1532..2422)
                     /locus_tag="VFV25_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY1391991.1"
                     /translation="MQVSIASSQYRLQAADLEVLLALTRSGTLADAARRLAVDASTVF
                     RSVQRIERQLGSRLFERSRSGYLPTEQALAIATHAERIEAELEAARAAVQRSDGAVTG
                     LVRITTTDTIAQGLVLPVLGPLKAQHPELRLELSATNELVNLTRRDADIAIRATRRPP
                     GHLVGRPLGTLRVAVYGRADRLGRGRIGALDGYPWIAPDEALPEHPTVRWRCKHLPRV
                     TPALLVNSISMVLDAVAAGMGIGAVPIFLAEQRPGLRALTEPLPECETELWLLTHPES
                     RHLRRIATVSAHLAQHVVLP"
     gene            2499..>2924
                     /locus_tag="VFV25_01370"
     CDS             2499..>2924
                     /locus_tag="VFV25_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEY1391992.1"
                     /translation="MKLYFTPNACSLAVHIVAREAGLALDLTKVDLMTHTLEDGSDFH
                     AVNPRGYVPVLQLDDGSRHTEVSALIQHLGDQDPIGRLMPRHGSRERFEVQQWISFVS
                     SELHKAFSPWLWHKETADSTVKAVKEKIATRFAELDQRLA"
BASE COUNT          512 a         1016 c          963 g          433 t
ORIGIN      
        1 cactcggccg ggcgccgcgg cttcggcaat gcagacgtga tcctcaacct gatgccggac
       61 cgcttcgcca atggcaatcc ggccaacgac gacgtgccag gctttgccga caaggccaat
      121 cgcgccgaca acgacgccgg ccgccacgga ggcgacatcc agggcatcgt cgatcacctc
      181 gaccacatcg ccgcgatggg ctacacgatg ctgtggccca cgccgctgac cgagagcaac
      241 cagccgcact actcctacca cggctacgcg gccaccgaca cctaccgcat cgacccgcgc
      301 tacggcagca acgaggacta ccgccgcatg gtggcggcgg cgcgcgagcg cggcatcggc
      361 gtcatccagg acatcgtgct gaaccacatc ggctcgaacc actggtggat gcgcgatttg
      421 ccgacgcagg attgggtcag cggcggcagc cactacgtgc ccaccgggca cgcacgcacc
      481 gcgatcagcg acccctatgc tgccgaggtc gaccgcgaga gcttcatcgc cggctggttc
      541 ggccccgaga tgcccgacat gaaccagcgc aacccgctgc tggcgaccta ccagatccag
      601 aacacgatct ggtggatcga gtacgcgggg ctggcaggca tacgcgccga cacctatggc
      661 tattccgata agcacttcct cgcgcaatgg tcgggccgcg tgatgctgga gtacccacgg
      721 ctcaacatcg tcggcgagga gtggagcaac aacccggtgg tgacctcgta ctggctgcgc
      781 ggcaagaaga acgccgacgg ctatgtctcc aacctaccca gcgtgatgga cttcccgctg
      841 caggccacgc tgctgcgcgc gctggtggcg ccggaagggc tgcattcggg gctggtggac
      901 gtctacgagg ccatggtcaa tgaccggctg tatcccgacc cgatgaacct cgtgctcttc
      961 gagggcaacc acgacctgcc gcgcctgtac agcatggtcg acgaggacct cgcgctatgg
     1021 aagatggcgc tcgccttcgt gctgaccgcg ccgcgcattc ctcagattta cgtcggtacc
     1081 gagatcctga tgaccagccc caaagtgcgc gacgacggcg ccttccgcca cgacttccca
     1141 ggcggctggg cgggcgacaa ggtcaatgcc ttcaccggcg agggcctgag cgataggcag
     1201 aaggaggcgc aggcctggct caagcgcgtg ctgaactggc gcaagactca gcccgcggtg
     1261 caccgcggca agttcatgca ctaccagccg caggacggga tgtatgtcta cttccgctat
     1321 gacgcgaagg ggctggtgat ggtggtgctc aacaagaatg ccggcgacgc gacgttggcg
     1381 accgagcgct ttcgccaggt gttgccgcag ggggccagtg ggaccgatgt gcttacgggg
     1441 cggcactacg agctggggga tacgctggtg gtgccggcgc ggtcggtgct gatcctcgac
     1501 ctgaagaggc ccgggtagga accgctcccc ttcacggcag caccacatgc tgcgccagat
     1561 gcgccgacac ggtcgcgatg cgccgcagat gacgcgactc cggatgcgtc agcagccaca
     1621 gctcggtctc gcattcgggc agcggctcgg tcagggcgcg caggccgggg cgctgctcgg
     1681 ccaggaagat cggcaccgcg cctatgccca tgccggccgc caccgcgtcc agcaccatcg
     1741 agatgctgtt gaccagcagc gccggcgtga cgcgcggcag atgcttgcag cgccagcgca
     1801 ccgtggggtg ctcgggcaag gcctcgtcgg gcgcgatcca cgggtaaccg tctagcgcgc
     1861 cgatgcggcc gcgtcccagg cggtcggctc ggccgtacac cgccacgcgc agcgtgccga
     1921 gcgggcgccc caccaagtgt ccgggtggcc gccgcgtggc gcggatcgcg atgtcggcgt
     1981 cgcgccgggt caggttgacg agctcgttgg tcgcgctgag ctccagcctc aactcggggt
     2041 gctgcgcctt cagcggcccc agcaccggca gcaccaggcc ctgcgcgatg gtgtcggtgg
     2101 tggtgatgcg caccagcccg gtcaccgcac cgtcggaccg ctgcacggca gcgcgcgcgg
     2161 cctccagctc ggcctcgatg cgctcggcat gcgttgcgat ggccaatgcc tgctcggtgg
     2221 gcaggtagcc gctgcgcgag cgctcgaaca aacggctgcc cagctggcgc tcgatgcgct
     2281 gcaccgagcg gaacaccgtg gacgcatcca cggccagccg ccgcgccgcg tcggccagcg
     2341 tgccggagcg cgtgagcgcc agcagcacct ccagatcggc cgcctgcagc ctgtattgcg
     2401 aacttgcaat cgacacttgc aagctatcct attttcattg cgtggctgca atcctactct
     2461 tggctcatcc caacccctga tcacgaaaga ccgtgacgat gaagctctac ttcaccccca
     2521 acgcctgctc gctggccgta cacatcgtgg cgcgcgaggc cggcctcgcc ctcgacctga
     2581 ccaaggtcga cctgatgacc cacacgctgg aagacggcag cgatttccac gccgtcaatc
     2641 cgcgcggcta tgtgccggtg ctgcagctgg acgacggcag ccgccacacc gaggtgagcg
     2701 cgctgatcca gcacctgggc gaccaggacc cgatcggccg gctgatgccc cggcacggca
     2761 gccgcgagcg cttcgaggtg cagcagtgga tcagcttcgt cagcagcgag ctgcacaagg
     2821 ccttcagccc ctggctgtgg cacaaggaga cggccgattc caccgtgaag gccgtgaagg
     2881 agaagatcgc cacgcgcttc gccgagctcg accagcggct ggcc
//
LOCUS       DASUCY010000066         5035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1591613, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000066 DASUCY010000000
VERSION     DASUCY010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 5035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5035
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1591613"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VFV25_01375"
     CDS             <1..199
                     /locus_tag="VFV25_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="antitermination regulator"
                     /protein_id="HEY1391993.1"
                     /translation="MPLSTPDIRAGLAEASQTWEALLAGGQRADQPQGRVALAAASEA
                     LLELFDRLTLLYERSMQGLMG"
     gene            358..780
                     /locus_tag="VFV25_01380"
     CDS             358..780
                     /locus_tag="VFV25_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014900079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HEY1391994.1"
                     /translation="MTTLKGSKTEQNLKDAFAGESQANRRYLYFAGKADVEGQNDVAA
                     LFRSTAEGETGHAHGHLEFLEAVGDPATGLPIGPTRANLKSAVAGETHEYTDMYPGMA
                     KTARSEGFDEIADWFETLAKAERSHANRYQKALDALVD"
     gene            996..2360
                     /locus_tag="VFV25_01385"
     CDS             996..2360
                     /locus_tag="VFV25_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEY1391995.1"
                     /translation="MSQREGNLEAPIRHPVDWKNPEFYEEDKCLQELGRIFDICHGCR
                     RCVSLCHAFPTLFDLVDATDDGEVHGVNKQDFWKVVDQCYLCDLCYMTKCPYVPPHAW
                     NVDFPHTMLRAKAIKFRKGEVKAGERFLASTDVHGQFAGIPIVVQAVNAIGKTKPARR
                     AMQSTLGVDENAWLPELATQRFRWSAPKSAATVVTNGERTPGKVAIFATCYVNYNEPG
                     IGHDLLKVLAHNGIPYVLVEKEKCCGMPKLELGDLDTVEKHKDANIPVLVKYAKEGYA
                     ILSAVPSCTLMFKQELPLMFPKDEDVKLVQDAMWDPFEYLVARSKDGLLKTDFKTALG
                     KVSYHVPCHGRVQNIGRKTEEMFKIIARSVEVQINTVERCSGHAGTYGVKQPYHQIAM
                     KIGKPVFKAMAKQDADGKGPDYISSDCALAGHHIAQGMANNQLPAAALRHPLSLVRIA
                     YGIE"
     gene            2384..2962
                     /locus_tag="VFV25_01390"
     CDS             2384..2962
                     /locus_tag="VFV25_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HEY1391996.1"
                     /translation="MTISRESLLTLEAYARVRQEMKARVIPHRRLRSVRLGEHLNVQF
                     EDETTIRYQIQEMLRVEKIFEEDGILQELEAYAPLVPDGSNWKATLLLEYPDVNERKR
                     ELARLIGVEDRLYVEVEGHGRVYAIADEDLDRENEEKTSSVHFLRFEFTPSMIEAVRA
                     GAGVTLGCDHRNYPAHVAIEPETLASLAGDLK"
     gene            complement(2974..4449)
                     /locus_tag="VFV25_01395"
     CDS             complement(2974..4449)
                     /locus_tag="VFV25_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1391997.1"
                     /translation="MPTRLLRLLAAAVALAPLAAQAQTVPAASQMPMSTMLGFEQLRL
                     PGSERLGLVGASLLFRTDSGWWLGPAVYGAATGERGGFFVGGAELQRRWTLVDHTQLV
                     TGLYVGGGGGGAAPVGGGLMLRPALTLLQDFGGWQAGLSLSDVRFPSGDIHSHQLGLL
                     LAWDGSYRYYDTALIGRPSFDIERSGLGLDRAGITLSQYRLPDGRHVDLVGVRAEQND
                     EASGLHGGIEAAAAAKGGAAGYMELLGSLGWEQALLPQALPSLKLGLRGAVGLGGGGG
                     VPNGGGAMAKAAATVGWQLVPGWRLALEAGIVRGWHGLPHERSAQIWLATDLGDSHRP
                     SVLGATGEIQRNEWAFSLQHVQHADRKTGGRGSLDTLGLKLDHYLGDSFYLSGQAHSA
                     YAGGAGAYSVGLLGAGLATPAADRRWQLGAEVLAGAAGGGGVATGGGAIVQGLGWAAW
                     RVGPSSQLRLGAGKVRSARSGGLNSPLVELAWSQSFGLTGR"
     gene            4620..>5035
                     /locus_tag="VFV25_01400"
     CDS             4620..>5035
                     /locus_tag="VFV25_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEY1391998.1"
                     /translation="MKTVGDKLEAFSVVGVKPGFNHHEENGVSAFETVTEKSFPGKWK
                     VIYFYPKDFTFVCPTEIVGFDKLAGQFAERDAVLLGGSTDNEFSKLGWRREHKDLNKL
                     GHYSFADVTGALIDQLGVRDKAAGVALRATFIVDPD"
BASE COUNT          938 a         1693 c         1671 g          733 t
ORIGIN      
        1 gatgccactg tccacccccg acatccgcgc gggcctcgcg gaagcgagcc agacctggga
       61 agccctactg gcaggcgggc agcgcgctga ccagccccag ggccgcgtcg cgctcgccgc
      121 ggcgagcgag gccctgttgg agctcttcga ccggctgacg ctcttatacg agcgcagcat
      181 gcagggcctg atgggctaag cccgtaacaa ccccaggcta tcgtgcggca taggcgcgag
      241 caattggact gagcccggca atcgggctag cctcacgcta catcccgcct tgccgctgtc
      301 atggcatggg cgcaggatgc gttgttggca gtcttcatca caggacagga gatcgaaatg
      361 acgacgctca agggctcaaa gaccgagcag aatttgaaag acgccttcgc cggcgaatcc
      421 caggcgaacc ggcgttacct ctatttcgcg ggcaaggccg atgtcgaggg ccagaacgat
      481 gtggcggcgc tgtttcgctc cacggccgaa ggcgagaccg gccatgcgca tggccacctc
      541 gagttcctcg aagcggtggg cgacccggcc accgggctgc cgattggccc gacgcgtgcc
      601 aacctgaagt cggccgtcgc cggcgagacc cacgagtaca ccgacatgta cccgggcatg
      661 gccaagacgg cgcgcagcga gggcttcgac gagatcgccg actggttcga aaccctggcc
      721 aaggccgagc gctcgcacgc caaccgctat cagaaggctt tggacgccct agtggattga
      781 tggtccaccc ccggagcggc tgacgccgct ccccctcaag ggggctgagc ccggacatga
      841 aaggtccagt gaacgtttca tgcctggcga agggccgggc cgcgggcccg gcgcggcctg
      901 caaggcacgc cagcggaccg gcagagccgg atccgcggcg tccgcttggc aagaccgggg
      961 cttgccgcgg gcccacacca caggagacat gagccgtgag ccagcgcgag ggcaatctcg
     1021 aggcgccgat ccgccatccc gtggattgga agaacccgga gttctacgaa gaagacaaat
     1081 gcctccagga gctggggcgc atcttcgaca tctgccacgg ctgccggcgc tgcgtgagcc
     1141 tgtgccatgc ctttcccacg ctgttcgacc tggtcgacgc cacggacgac ggcgaagtgc
     1201 acggcgtgaa caagcaggat ttctggaagg tcgtggacca gtgctatctc tgcgacctct
     1261 gctacatgac caagtgcccc tatgtgccgc cgcatgcgtg gaacgtggac ttcccgcaca
     1321 ccatgctgcg cgccaaggcg atcaagttca ggaagggcga ggtcaaggcc ggtgagcgct
     1381 ttctcgcttc caccgatgtg cacggccagt tcgccggcat tcctatcgtc gtgcaggcgg
     1441 tgaacgcgat cggcaagacc aagccggcgc gccgtgcgat gcagtcgacg ctgggcgtgg
     1501 acgagaacgc ctggctgccc gagctggcca cgcagcgctt ccgctggagc gcgcccaaga
     1561 gcgcggcgac ggtggtcacc aatggcgagc gcacgccggg caaggtggcg atctttgcca
     1621 cctgctacgt caactacaac gagcccggca tcggccatga cctgctcaag gtgctggcgc
     1681 acaacggcat tccctacgtg ctggtcgaga aggaaaaatg ctgcggcatg cccaagctgg
     1741 agctgggcga cctcgatacg gtggaaaagc ataaggacgc caacatcccg gtgctggtga
     1801 aatacgcgaa ggagggctat gccatcctga gcgccgtccc ttcgtgcacg ctgatgttca
     1861 agcaggagct gccgctgatg ttccccaagg acgaggacgt caagctggtg caggacgcga
     1921 tgtgggatcc tttcgaatac ctcgtcgcgc gcagcaagga cggcctgctg aagaccgact
     1981 tcaagaccgc gctcggcaag gtgagctatc acgtgccctg ccatggccgg gtgcagaaca
     2041 tcggccgcaa gaccgaggag atgttcaaga tcatcgcgcg cagcgtcgag gtccagatca
     2101 acaccgtcga gcgctgctca ggccatgcag gcacctacgg cgtcaagcag ccctatcacc
     2161 agatcgcgat gaagataggc aagccggtct tcaaggccat ggccaagcag gacgccgacg
     2221 gcaaaggccc tgactacatc agctccgact gcgccctcgc cggccaccac atcgcgcagg
     2281 gcatggccaa taaccagctg ccggccgcgg cgctgcgcca ccccttgagt ctggtgcgta
     2341 tcgcgtatgg aatcgaatga gccggacaga gagaggaggc ggcatgacca tcagtcgtga
     2401 gagcctgttg acgctcgagg cctatgcccg ggtgcgccag gagatgaagg cgcgcgtcat
     2461 tccccaccgc cgcctgcgca gcgtgcgcct gggcgaacac ctcaacgtgc agttcgagga
     2521 cgagacgacg atccgctacc agatccaaga gatgctgcgc gtcgagaaga tcttcgagga
     2581 agacggcatc ctgcaggagc tcgaggccta tgcgcccctg gtgcccgacg gcagcaactg
     2641 gaaggcgacc ctgctgctcg aataccccga cgtgaacgag cgcaagcgcg agctagcgcg
     2701 gctgatcggg gtggaggacc gcctgtacgt cgaggtcgag ggccatgggc gcgtctacgc
     2761 cattgccgac gaagacctgg accgcgagaa cgaggaaaag acctcgtcag tgcatttcct
     2821 gcgcttcgag ttcacacctt cgatgatcga ggcggtgcgc gccggggccg gcgtcacgct
     2881 gggctgcgac caccgcaact acccggccca tgtggcgatc gagcccgaga cgctggccag
     2941 cctggccggt gacctcaagt aagagttttg ccgtcagcgg ccggtgaggc cgaagctctg
     3001 gctccaggcc agctcgacca gcgggctgtt gaggccgccg ctgcgcgccg agcgcacctt
     3061 gccggcgccg agccgcagct ggctgctcgg cccgacgcgc caggctgccc agcccaggcc
     3121 ctgcacgatt gcgccgcccc cggttgccac accgccgccg cccgccgcgc cggccagcac
     3181 ttcggcgccg agctgccagc ggcgatccgc tgccggtgtc gccagcccgg cgccgagcag
     3241 gcccacagaa taggcgcctg cgccgccggc ataggcgctg tgcgcctggc cgctgaggta
     3301 gaagctgtcg cccagatagt gatcgagctt cagccccagg gtatcgagcg agccgcggcc
     3361 gccggtcttg cgatcggcgt gctgtacatg ctgcaggctg aaggcccatt cattgcgctg
     3421 gatctcgccg gtggcgccga ggaccgatgg gcgatgactg tctcccagat cggtagcgag
     3481 ccagatctgt gcgctgcgtt catgaggcag gccgtgccag ccgcgcacga tgcctgcttc
     3541 cagcgccagc cgccagccgg gcacgagttg ccagcccacg gtcgcggcgg ccttggccat
     3601 cgcgccgccg ccattgggta cgccgccgcc gccgcccagg cccacggcgc cgcgcaggcc
     3661 cagtttcagc gagggcaggg cttgcggcag caaggcctgc tcccagccca ggctgcccag
     3721 caattccatg tagcccgccg cgccgccctt ggccgcggcg gcggcctcga tgccgccatg
     3781 caggccgctg gcttcgtcgt tctgctcggc gcgcacgccc acgagatcga cgtggcgccc
     3841 gtctggcagc cggtattggc tcagcgtgat gcccgcccgg tccagaccca ggccgctgcg
     3901 ctcgatgtcg aaggacggcc ggccgatcag agcggtgtcg tagtagcggt agctgccgtc
     3961 ccaggccagc agcagcccga gctggtgact gtggatgtcg ccgctcggaa agcgcacgtc
     4021 tgacaaggag agaccggcct gccagccacc gaagtcctgc agcagcgtga gcgcagggcg
     4081 cagcatcagc ccgccgccca ccggcgccgc tccgccacca ccgccgccga cgtagaggcc
     4141 ggtgaccagc tgcgtgtggt cgacaagcgt ccagcgccgc tgcagctcgg cgccgccgac
     4201 gaagaagccg ccgcgctcac cggtggcggc gccatagacc gccgggccta gccaccagcc
     4261 ggagtcggtg cgaaacagca gcgacgcgcc gaccagcccg aggcgttcgc tgccaggcag
     4321 gcgcagctgc tcgaagccca gcatggtcga catcggcatc tgactggccg cgggcacggt
     4381 ctgggcttgc gcggcgaggg gcgctagcgc gacggcggcg gcgagtaggc gaaggaggcg
     4441 ggtgggcatg gcgtgctgaa ctgaaagggc agcattcgac cacagcccgg cgcttcgcgg
     4501 ccggctatct ccggtttagg cagagccaat cgggtggcaa tgcgcttgcc aatacaatct
     4561 atcgaaacgg acgtatcaat agcaatctcc gtcaaaaccc aaaccgaaag gaaatcacca
     4621 tgaaaaccgt gggcgacaag ctcgaagcct tctccgtcgt cggcgtcaag cccggcttca
     4681 accaccacga ggaaaacggc gtctcggcct ttgaaaccgt caccgagaag agcttcccgg
     4741 gcaagtggaa ggtgatctac ttctatccca aggacttcac cttcgtctgc ccgaccgaga
     4801 tcgtcggctt cgacaagttg gccggccagt tcgccgagcg cgatgccgtg ctgctgggcg
     4861 gctcgaccga caacgaattc agcaagctgg gctggcgccg cgagcacaag gacctgaaca
     4921 agctgggcca ctacagcttc gccgacgtga ctggcgcgtt gatcgaccag ctgggcgtgc
     4981 gcgacaaggc cgcaggcgtg gcgctgcgcg cgaccttcat cgtcgatccc gacaa
//
LOCUS       DASUCY010000067         5520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_97685, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000067 DASUCY010000000
VERSION     DASUCY010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 5520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5520
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97685"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..323
                     /locus_tag="VFV25_01405"
     CDS             18..323
                     /locus_tag="VFV25_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HEY1391999.1"
                     /translation="MNTPLLQYYEAIEQASTDMLSAAREGDWDRVVKLEGACAVLISR
                     LKQAAAGLPLAAEESRLKARIMQRILVNDAEIRTLAEPWLEDLDRLMGGRQPRTFLH"
     gene            502..1248
                     /locus_tag="VFV25_01410"
     CDS             502..1248
                     /locus_tag="VFV25_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar regulator YcgR PilZN domain-containing
                     protein"
                     /protein_id="HEY1392000.1"
                     /translation="MPAPLDAMGSLRSGALDEFRIHSPVEVQALLKQLQDSNALLHLS
                     TSDGVSYTTTLWAQDRARGILSFSADARDPRLLPLLESDEAVVVGYADNIKLQFDARK
                     LVLVQSARAAAINAAYPYEIFRFQRRGCYRVRPLARSMAVAAVPTSQAAGGRMQLRVI
                     DVSLTGVGLFLPDSAPRPRPGDILEQVSLQLDAETQLNVNLEVRHVTVAHPDHRGARM
                     GCEMLDLSRDAARELQRYIDRTQAQRRMLT"
     gene            complement(1261..1578)
                     /gene="fliE"
                     /locus_tag="VFV25_01415"
     CDS             complement(1261..1578)
                     /gene="fliE"
                     /locus_tag="VFV25_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HEY1392001.1"
                     /translation="MDVRLKPFDFAQAAARAGMPQVAQRVKESTQAAGFQQSFTQALR
                     SVSDTQNQAAHMQREVQLDNPTVSLEETMVAMQKAQIGFQAALTVRNRLVQAYTDIMN
                     MPV"
     gene            1752..3419
                     /gene="fliF"
                     /locus_tag="VFV25_01420"
     CDS             1752..3419
                     /gene="fliF"
                     /locus_tag="VFV25_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HEY1392002.1"
                     /translation="MDHVAAQNLPATAAYGSNATPGFGQRLAALPAKRKLTLGAGLAA
                     LVAIVAAMLMMNSQGDYKVLYANLSDKDGGAVIAQLSQMNVPYKHAEGGSAILVPASM
                     VHDARLKLASAGLPKGSVVGFELMDNARFGITQFQERLTFQRGLEGELTRSITALSAV
                     QAARVHLALPNQNGFFREQQKPSASVMLTLYPGRTLDRAQLAGIVHLVSSSVPELSPQ
                     AVSVLDQTGTLLSGSSDSGGTGGLDAQQLQYVNQIEANYTKRIVDILEPVVGRDNLRA
                     QVAAEVDFSQTESTSEEFKPNQGQNPAAVRSQQSSSSIAGGVGAQPSGVPGAASNQPP
                     VPATAPINGPAQPLQAAQGGAGSSGPARTEAMTNYEVDKTVRVTRNATGTVKHLNAAV
                     VINHHAVTDAKGKTSYVPLGKDELDKLTALVQESIGFNQERGDSVKVINAPFRVEKPD
                     AVELPLWKQPELLDLLRSAAVPGALTLLALIVVFGAIRPALKAARSSRPKLNAVVDDV
                     QSLPAANLLPVLDAPQSDNRLDVARRLAKDNPGAVANIVRGWVAKEA"
     gene            3462..4457
                     /gene="fliG"
                     /locus_tag="VFV25_01425"
     CDS             3462..4457
                     /gene="fliG"
                     /locus_tag="VFV25_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HEY1392003.1"
                     /translation="MDDQGLQDSAILLMSLGEEEAAEVFKHLAPKEVQRLGETIARMK
                     TVPRERVEQVLTRFGSDAAEQSMLVSDTDEYVKAVLRKALGEDKANLLIDRILQGSDV
                     TGIESLKWMDPGSVAELLRNEHPQIIAAILVHLDYEQGSAVLKCFAERQRNEVMVRIA
                     TLDGIQPSALKDLNEVMSKVLAGGDKLRKASLGGVKPAAEIINLMGNSIEATVLDYIR
                     EIDGELAQKIMDNMFVFDDLNKIDDRGIQALLKEVQSDSLIIALKGATPELREKIFRN
                     MSSRAAETLREDLESRGPVRLSEVEAEQKEMLKIVRRLADEGQVVLGGGGDDQFV"
     gene            4438..5169
                     /locus_tag="VFV25_01430"
     CDS             4438..5169
                     /locus_tag="VFV25_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HEY1392004.1"
                     /translation="MTTSSFKRRSARQVPPPQAGVDAAKTAYARFIPREELHSFAAWR
                     PGALGGDGVQAEPAAPNEADLAAQTQAARQAGYQDGYRDGLAALEGFKQSYAAQVTAQ
                     LASIAGAFQQQLGALEQDLAHRLAGVALSLARQVVRSELNQRPQLVVDVAQEALGALL
                     LSARHVSMRVNPDDHALVAQGTQELMAARGARLLPDAQVTRGGCVVESDIGVIDASVE
                     MRWRRAAEAMGRESNWTASEDEGLS"
     gene            5166..>5520
                     /locus_tag="VFV25_01435"
     CDS             5166..>5520
                     /locus_tag="VFV25_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HEY1392005.1"
                     /translation="MTAPMDQWMRYLDDLHGCASAPLPLQVQGTLVRVAGLVLEAAGI
                     RVPVGSVCEVRMEGALPVPAEVVGFAGDRAYLMPTAEVHGLASGARVVPLAPPVAAPM
                     LGQASHPWRRSEDRTR"
BASE COUNT          932 a         1888 c         1901 g          799 t
ORIGIN      
        1 caaaccagga gcccttcgtg aacaccccgc tcttgcagta ttacgaggcg atcgagcaag
       61 ccagtacgga catgctctcg gccgcccgcg aaggcgactg ggaccgcgtg gtcaagctcg
      121 aaggcgcttg cgcggtgctg atctcccggc tcaagcaggc cgccgccggc ctgccgctcg
      181 ctgccgagga gagccgactg aaggcgcgca tcatgcagcg catcctggtg aacgatgccg
      241 aaatccgcac gctcgccgaa ccctggctgg aagacctgga tcgcctgatg ggcggccgcc
      301 agccgaggac tttcctgcac tgaactaagc ggtggcggcc atgagaccgg agaacgggtc
      361 agggcacttg gtgaagtcgg cgagcggggt gtcgtcggca cgagccgccg cgccgggccg
      421 ctcccaagca aggcccgttc ccctctcgga ggggtggtcg ccgtactcgg cgagcggggt
      481 gtcgtcatga ccatggagac catgcccgcc ccgctcgacg cgatgggcag cctacgcagc
      541 ggcgcactcg acgagttccg gattcattcg ccggtggaag tgcaggcgct cctgaagcag
      601 ctgcaggaca gcaatgcgct gctgcatctg agcacgtcgg acggcgtcag ctacacgacg
      661 acgctgtggg cgcaggaccg cgcgcgcggc atcctcagct tcagcgccga tgccagggat
      721 ccgcgcctgc tgccgctgct cgaatcggac gaggcggtgg tggtcggcta tgcggacaac
      781 atcaagctgc agttcgacgc gcgcaagctc gtactggtgc agagcgcgcg cgccgcggcc
      841 atcaacgccg cctatccgta cgagatcttc cgcttccagc gccgcggctg ctaccgcgtg
      901 cgcccgctgg cgcgcagcat ggccgtggcc gccgttccca cgtcacaggc cgcgggcggc
      961 cgcatgcagc tgcgcgtgat cgacgtcagc ctgaccggtg tcggactctt cctgcccgac
     1021 agtgcgccac ggccgcggcc gggcgacata ctggagcagg tgtcgctgca actcgacgcc
     1081 gagacgcagc tgaacgtgaa cctggaagtc cgccatgtca ccgtggccca tcccgaccac
     1141 cgcggcgccc gcatgggctg cgagatgctc gatctcagcc gcgacgccgc gcgcgagctg
     1201 cagcgctata tcgacagaac gcaggcgcag cggcgcatgc tgacttgacc accccctacg
     1261 tcacactggc atgttcatga tgtcggtata ggcctgaacg agccggttgc gcaccgtcag
     1321 cgcggcctgg aagccgatct gtgccttctg catcgccacc atggtctcct cgaggctgac
     1381 cgtggggttg tccagctgca cctcgcgctg catgtgcgcc gcctgattct gcgtgtcgct
     1441 caccgagcgc agcgcctgcg tgaagctttg ctggaagccg gccgcctgcg tgctttcctt
     1501 cacgcgctga gccacctgcg gcatgcccgc gcgcgccgcg gcctgcgcga aatcgaaggg
     1561 cttgagtctc acgtccatgg gcggtctccg ggcacgatgg cttagaccgg atcgtagcga
     1621 ggcaatggcc ttgcctatgg cgcgaactgg gcctcgtttg catggttgtt cccggcattt
     1681 gtttccggtg gcctccgaat aatcctctcg gcgtgctcgc ttgtgccgag cgccctgggg
     1741 aagaccaaga catggaccac gtcgccgcgc aaaacctgcc cgccaccgcc gcttacggca
     1801 gcaacgccac acccggcttc ggccagcgcc tggccgcgct gccggccaag cgcaagctga
     1861 cgctgggcgc cggcctggcg gccctggtcg ccatcgtcgc cgcgatgctg atgatgaaca
     1921 gccagggcga ctacaaggtg ctgtacgcca acctgtccga caaggacggc ggcgccgtca
     1981 tcgcccagct ctcgcaaatg aacgtgccct acaagcacgc cgaaggcggc tcggccatcc
     2041 tggtgccggc gagcatggtg cacgacgcgc gcctgaagct cgcctcggcc ggcctgccca
     2101 agggctcggt ggtcggcttc gagctgatgg acaacgcgcg cttcggcatc acccagttcc
     2161 aggagcgcct gaccttccag cgcggcctgg aaggcgagct cacgcgctcg atcaccgcgc
     2221 tgtcggccgt gcaggccgcg cgcgtgcacc tggcgctgcc caaccagaac ggcttcttcc
     2281 gcgagcagca gaagcccagc gcctcggtga tgctgacgct gtatccgggc cgcacgctgg
     2341 accgcgcgca gctggccggc atcgtccatc tcgtctcctc cagcgtgccc gagctcagcc
     2401 cgcaggcagt gagcgtgctc gaccagaccg gcaccctgct gtccggctcg tcggacagcg
     2461 gcggcaccgg cggtctcgat gcacagcagc tgcaatacgt caaccagatc gaggccaact
     2521 acaccaagcg catcgtcgac atcctggagc cggtggtcgg gcgcgacaac ctgcgtgcac
     2581 aggtcgcagc cgaagtcgat ttctcgcaga ccgagtcgac ctccgaggag ttcaagccca
     2641 accaggggca gaacccggct gcggtgcgca gccagcagtc ctcgtcctcc atcgccggcg
     2701 gcgtcggcgc ccagcccagc ggcgtgcccg gcgcagcctc caaccagccg cccgtgccgg
     2761 ccaccgcgcc gatcaacggc ccggcgcagc cgctgcaggc ggcccagggc ggcgcgggtt
     2821 cgagcggccc ggcccgtacc gaggccatga ccaactacga agtcgataag accgtgcgtg
     2881 tgacgcgcaa tgccaccggc accgtgaagc acctgaacgc cgcagtggtg atcaaccatc
     2941 atgccgtgac cgatgccaag ggcaagacca gctacgtgcc gctcggcaag gacgagctcg
     3001 acaagctgac cgcgctggtg caggaaagca tcggcttcaa ccaggagcgc ggcgattcgg
     3061 tgaaggtcat caatgcgccg ttcagggtcg agaagcccga cgccgtcgag ctgccgctgt
     3121 ggaagcagcc cgagttgctt gacctgctgc gcagcgccgc ggtcccgggt gcgctcacgt
     3181 tgctggccct gatcgtggtg ttcggcgcga tccgccccgc gctgaaggcc gcgcgcagca
     3241 gccggccgaa gctgaacgcc gtggtggacg acgtgcaaag cctgcccgcc gccaacctac
     3301 tgcccgtgct ggatgcgccg cagagcgaca accgcctgga tgtggcgcgg cgcctggcca
     3361 aggacaaccc cggcgcggtc gccaacatcg tacgcggctg ggtggccaag gaagcatgag
     3421 gcgcgttctc ttgcggcaat ccgacgacag ggaagaagaa catggatgac cagggcctcc
     3481 aagactccgc gatcctgctc atgtcgctgg gcgaggaaga ggcggccgaa gtcttcaagc
     3541 atctcgcgcc caaagaagtg cagcgcctgg gcgagacgat cgcgcgcatg aagaccgtgc
     3601 cgcgcgagcg cgtcgagcag gtgctcacgc gcttcggcag cgatgccgcg gaacagagca
     3661 tgctggtgag cgacaccgac gagtacgtca aggccgtgct gcgcaaggcc ctgggcgagg
     3721 acaaggccaa cctgctgatc gaccgcatcc tgcagggcag cgacgtcacc ggcatcgaga
     3781 gcctcaaatg gatggacccg ggttcggtgg ccgagctgct gcgcaacgag cacccgcaga
     3841 tcatcgccgc catcctggtg catctggact acgagcaggg ctccgcggtc ctgaagtgct
     3901 tcgcggagcg ccagcgcaac gaggtgatgg tgcgcatcgc gacgctcgac ggcatccagc
     3961 cctcggcgct gaaggacctg aacgaagtga tgagcaaggt gctggccggc ggcgacaagc
     4021 tgagaaaggc ctcgctgggc ggcgtgaagc cggccgccga gatcatcaac ctgatgggca
     4081 acagcatcga ggcgaccgta ctcgactaca tccgcgagat cgacggcgag ctggcgcaga
     4141 agatcatgga caacatgttc gtgttcgacg acctgaacaa gatcgacgat cgcggcatcc
     4201 aggcgctgct gaaggaagtg cagagcgatt cgctcatcat cgcgctcaag ggcgccacgc
     4261 ccgagctgcg cgagaagatc ttccgcaaca tgtcctcgcg cgccgccgag acgctgcgcg
     4321 aggacctcga gtcgcgcggc ccggtgcgcc tatcggaggt cgaggccgag cagaaggaga
     4381 tgctgaagat cgtccgccgc ctggcggacg agggccaggt cgtgctggga ggcggcggtg
     4441 acgaccagtt cgtttaagcg ccgctcggcc cggcaggtcc cgccgccgca ggccggcgtc
     4501 gatgccgcca agacggccta tgcgcgcttc attccgcgcg aggagctgca ttctttcgcg
     4561 gcctggaggc caggtgcgct gggcggcgac ggcgtgcagg cggagccggc cgctccgaac
     4621 gaggcggacc tcgccgcgca gacgcaagcg gcacggcagg ccggctatca ggacggctac
     4681 cgcgacggcc tggccgcgct cgaaggcttc aagcaaagct atgccgcaca ggtgacggcc
     4741 cagctggctt ccatcgccgg tgccttccag caacagctgg gagcactgga gcaggacctg
     4801 gcgcacaggc tggctggcgt ggccctgtcg ctggcgcgcc aggtggtgcg cagcgagctg
     4861 aaccagcgcc cgcagctggt ggtcgacgtg gcgcaggaag cgctcggcgc attgctgctg
     4921 tcggccaggc acgtcagcat gcgcgtgaac cccgacgacc acgcgctggt agcgcaaggc
     4981 acccaggagc tgatggctgc gcgcggcgcc cgtttgctgc ccgatgcgca ggtcacgcgc
     5041 ggcggctgcg tcgtcgaatc ggacatcggc gtgatcgacg cgagtgtgga aatgcgctgg
     5101 cgccgcgccg cggaagccat ggggcgcgag tcgaactgga cggccagcga ggacgagggg
     5161 ctgtcatgac cgcgcccatg gaccagtgga tgcgctatct cgacgacttg cacggctgcg
     5221 ccagcgcgcc gctgccgctg caggtgcagg gcacgctggt gcgcgtggcc ggcctcgtgc
     5281 tggaggcggc cggcattcgc gtgcccgtgg gttccgtgtg cgaagtgcgc atggaaggcg
     5341 ccctgccggt gcccgccgag gtggtgggct tcgccggcga tcgcgcctat ttgatgccca
     5401 ccgccgaggt gcatggcctg gccagcgggg cgcgcgtggt gccgctggcc ccgccggtgg
     5461 cggcgccgat gctggggcag gccagccacc cctggcgccg cagcgaggat cgcacccgcc
//
LOCUS       DASUCY010000068         2934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1158011, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000068 DASUCY010000000
VERSION     DASUCY010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158011"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..2029
                     /gene="hyfB"
                     /locus_tag="VFV25_01440"
     CDS             5..2029
                     /gene="hyfB"
                     /locus_tag="VFV25_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HEY1392006.1"
                     /translation="MGSLSSPADWLLLDWLLLAGAGWLLVGAIGVLLLHRLGWVAHVL
                     FPIGGLIGLALAGLSLAALHGTPEVAVLPIGLPGLPFHLRLDSLAAYFLLIIGAASAG
                     ISWFAAGYFRRGEGTPPGLLCLEYHVFLASMAGVVLADDAYAFMVMWESMALASYFLV
                     TANHRVAEVRSAGYLYITMARVGAIGILLCFGVLQANTGDYTFANMRAQQLSAFWASL
                     GFGLALFGFGAKAGILPLHVWLPEAHPAAPSPVSALMSGVMLNTAIYGLLRVSLDLLH
                     LRLWWWGALLLAAGLITALYGVVFAAVQTDMKRLLAYSSIENMGLLFVGMGLTLIFAG
                     YDMRPMAALSLTATLYHVASHAFFKSLLFLGTGSVLHATAERSLGKLGGLIRMMPWVG
                     WLSLLGVLTSAGLPPLGGFVSEWLLLQSFLFTPSLPVPLLTMLVPVVAALIALVAALA
                     GYTMVKFFGVIFLGLPREEKLAQAHDAGVRERIGMGWLAAGCLALGLLPVPFITLIDP
                     VTQQLVGASLAGRMAGHSWLLVPNSVQQASYGPVIFLLGILGSFALAFLLVRRLYHGR
                     LRRSIAWACGFPWGTARMQDSAEGFGQPIRQIFEPFFQMQRELPTPFDAEPHYRVVVE
                     DHFWRWLYLPIARAANSLARLIGVMQQGRIAVYLLYSFVTLAATLLAVMH"
     gene            2035..>2934
                     /locus_tag="VFV25_01445"
     CDS             2035..>2934
                     /locus_tag="VFV25_01445"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004554989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEY1392007.1"
                     /translation="MPSISGLLSQVLEIVLAIAIAPLLTGWVNQWRAWLQNKSAPPVW
                     QPYLMLHKLFNKESVVADNASRLFRTVPYLVFGCMLLASAIVPTLSTDLPLAPAADAI
                     ALVGLFALARVFISLAAMDVGTAFGTLGARREMLIGFLAEPALLMVLFSASLISKSTS
                     LTTIVDTLAHRELAIYPGLAFAGVAFTMVSLAENARVPVDNPATHLELTMIHEALVLE
                     YSGRHLALLEWAASLKLFAYSCIGLALFFPWGVAQAQAPLAMLLALPALVFKLAIGGF
                     LLALLETLSAKMRIFRVPEFLATA"
BASE COUNT          334 a         1035 c          979 g          586 t
ORIGIN      
        1 accgatgggc agcctgtcgt cgcccgccga ctggctgctg ctcgactggc tgctgctcgc
       61 cggcgcgggc tggctgctgg tgggggccat cggcgtgctg ctgctgcacc gcctcggctg
      121 ggtcgcgcat gtgctgtttc cgatcggcgg cctgatcggc ctggcgctgg caggcctctc
      181 gctggctgcg ctgcacggca cgcccgaggt ggcggtattg ccgatcggcc tgcccggcct
      241 gcctttccat ctgcggctcg acagcctggc cgcctatttc cttctcatca tcggcgcggc
      301 cagcgccggc atctcctggt tcgccgctgg ctatttccgc cgcggcgaag gtacgccgcc
      361 ggggctgctg tgcctggagt accacgtctt cctcgctagc atggccgggg tggtgctggc
      421 cgatgatgcc tatgccttca tggtcatgtg ggagtcgatg gcgctcgcgt cctacttcct
      481 cgtcaccgcc aaccaccgcg tggccgaggt gcgcagtgcg ggctatctct acatcacgat
      541 ggcgcgcgtg ggcgcgatcg gcattctgct gtgcttcggc gtgctacagg ccaacaccgg
      601 cgactacacc ttcgccaaca tgcgcgccca gcagctgtcg gccttctggg cctcgctcgg
      661 cttcggcctt gcgctgttcg gcttcggcgc caaggccggc atcctgccgc tgcacgtgtg
      721 gctgcccgag gcgcacccag cggcgccgtc gccggtctcg gcgctgatga gtggggtgat
      781 gctcaacacc gcgatctacg gcctgctgcg cgtgtccctg gacctgctgc acctgcgtct
      841 gtggtggtgg ggtgcgctgc tgctggctgc gggcctcatc actgcgctgt atggcgtggt
      901 cttcgccgcc gtgcagaccg acatgaagcg gctgctggcc tactcgtcga ttgagaacat
      961 gggcctgctc ttcgtcggca tgggcctcac cctgatcttc gcgggctacg acatgcggcc
     1021 gatggccgcg ctgtcgctca ccgcgacgct ctaccacgtg gcaagccatg ccttcttcaa
     1081 gagcctgctg ttcctgggca ctggcagcgt gctgcacgcg acggccgagc gcagcctggg
     1141 caagctcggc ggcctgatcc gcatgatgcc ctgggtcggg tggctgagcc tgctcggcgt
     1201 cctcaccagc gccggcctgc cgcccctggg cggcttcgtg tcggagtggc tgctgctgca
     1261 gagtttcttg ttcacgccca gcctgccggt gccgctgctg accatgctgg ttcccgtggt
     1321 ggcggcgctg atcgcgctgg tggcggcgct ggccggctac accatggtca agttcttcgg
     1381 cgtgatcttc ctcggcctgc cgcgcgagga gaaactggcc caggcccatg acgccggcgt
     1441 gcgcgagcgc atcggcatgg gatggctggc ggcgggctgc ctcgcgctcg gactgctgcc
     1501 ggtgcccttc atcacgctga tcgacccggt gacccagcag ttggtcggtg ccagcctcgc
     1561 cggccgcatg gccggccaca gctggctgct ggtgccgaac agcgtgcagc aggcgagcta
     1621 cgggccggtg atcttcctgc tcggcatcct gggcagcttc gcgctcgcct tcctgctggt
     1681 gcgccggctc tatcacggcc gcctgcggcg cagcatcgcc tgggcctgcg gctttccgtg
     1741 gggcacggcg cgcatgcagg actcggccga gggcttcggc cagcccatcc ggcagatctt
     1801 cgagcccttc ttccagatgc agcgcgagct gcccacgccg ttcgatgccg agccgcatta
     1861 ccgcgtggtc gtcgaagacc atttctggcg atggctgtac ctgcctatcg cgcgcgcggc
     1921 taactctctc gctcgcctga tcggcgtgat gcagcagggt cgcatcgcgg tctacctgct
     1981 gtacagcttc gtcacgctgg ccgccacgct gctggcagtg atgcactgat gaccatgccg
     2041 agcatcagcg gcctgctctc ccaggtgctc gagatcgtgc tggcgatcgc gatcgcgccg
     2101 ctcttgaccg gctgggtcaa ccagtggcgg gcctggctgc agaacaagtc ggcgccgccc
     2161 gtgtggcagc cctatctcat gttgcacaag ctgttcaaca aggagtcggt ggtcgccgac
     2221 aacgcctcgc ggctgttccg cacggtgccc tatctagtgt tcggctgcat gctgctggcc
     2281 agcgcgatcg tgccgaccct gtcgaccgac ctgccgctgg cccccgccgc cgacgcgatc
     2341 gcgctggtcg gcctgttcgc gctggcccgc gtcttcatct cgctggccgc gatggacgtc
     2401 ggcaccgcct tcggcacgct gggcgcgcgg cgcgagatgc tgatcggctt cctggccgag
     2461 ccggccctgc tgatggtgct gttctccgca tcgctgatct cgaagtccac ctcgctcacg
     2521 accatcgtcg acaccctggc gcaccgggag ctcgccattt atcccggcct cgccttcgcc
     2581 ggcgtggcgt tcacgatggt ctcgctggcg gagaacgcgc gcgtgccggt cgacaatccc
     2641 gccacccatc tcgagctgac gatgatccac gaggcgctcg tcctcgagta ctcggggcgg
     2701 cacctggcac tgctggaatg ggccgcgagc ctgaagctgt tcgcctactc gtgcatcggg
     2761 ctcgcgctgt tcttcccctg gggagtggcc caggcgcagg cgccgctggc gatgctgctg
     2821 gcgctgccgg cgctcgtctt caagctggcc attggcggct tcctgctcgc gctgctggag
     2881 acgctgtccg ccaagatgcg catctttcgc gtgcccgagt tcctcgccac tgcc
//
LOCUS       DASUCY010000069         2080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_901026, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000069 DASUCY010000000
VERSION     DASUCY010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901026"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /gene="queE"
                     /locus_tag="VFV25_01450"
     CDS             <1..599
                     /gene="queE"
                     /locus_tag="VFV25_01450"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HEY1392008.1"
                     /translation="GEGSHAGRPAVFCRFAGCNLWSGREADRASAVCKFCDTDFVGTD
                     GTLGGKFATAAELAGRVAAQWPSADLSARFVVLTGGEPLLQLDEALVAALHGQGFEIA
                     VETNGTVAAPAGIDWLCVSPKAGAPLVQRSGQEIKVVVPQEGLDLAELEKLDFEHRRV
                     QPMDGPQRQANTEWAVQWCLDHPRWQLSLQTHKYLGIR"
     gene            608..982
                     /locus_tag="VFV25_01455"
     CDS             608..982
                     /locus_tag="VFV25_01455"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEY1392009.1"
                     /translation="MFELSQSFYFESAHTLRRKVERNEADGSRRVHGHTYTAEVSVRG
                     QIDPETGMVVDLAVLRAAIAAVRAKLDHHFLDEVPGLGAPTLENLCVFIHKQLSAAVP
                     GVCKVSVERKSSGDRCSYRPAA"
     gene            979..>2080
                     /gene="bioA"
                     /locus_tag="VFV25_01460"
     CDS             979..>2080
                     /gene="bioA"
                     /locus_tag="VFV25_01460"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEY1392010.1"
                     /translation="MSEASLPERSLRAVWHPCTQMKRHEREPVLAIVRGEGPWLYDDQ
                     GRRYLDAVSSWWVNLFGHQHAGIKAALVDQLDRIEHVMLAGLTHEPVVALSERLAALT
                     GLGHAFYGSDGASATEIALKMSAHYWRNLGRPGKCEFIGLAGGYHGETVGALSVTDIA
                     IFREAYAPLVRLSAALPTPDARLAAPGVSPREHAEQCARALQAYLEAHHERTAALILE
                     PLVQCASGMAMHDAEYLRLARALCDRYQVHLIADEIAVGFGRTGTMFAHQQAGIRPDF
                     ICLSKGLTGGYLPLSAVLTTDTVYQAFYDDSTARGFLHSHSYTGNPLACRAALAVLDV
                     FERSPVLERNRAAAARLNQLSTVLAAHPRVRHA"
BASE COUNT          312 a          704 c          716 g          348 t
ORIGIN      
        1 agggcgaggg cagccatgcc gggcgcccgg cggtgttttg tcgcttcgcc ggctgcaacc
       61 tctggagcgg gcgcgaggcc gatcgcgcca gcgccgtctg caagttctgc gacactgatt
      121 tcgtcggcac cgacggcacg ctaggcggca agttcgccac ggcggccgag ctggccgggc
      181 gggtggcggc gcaatggccg agtgccgacc tcagtgctcg cttcgtcgtg ctgaccggcg
      241 gcgagccctt gctgcagctc gacgaggccc tcgtcgccgc gctgcatgga caaggcttcg
      301 agatcgccgt cgagaccaat ggcaccgtgg ccgcaccggc gggcatcgat tggctctgcg
      361 tcagccccaa ggcgggtgcg ccgctggtgc agcgctcggg ccaggagatc aaggtggtcg
      421 tgccgcaaga gggtctggat ctcgccgagc tggagaagct cgatttcgag caccgtcgcg
      481 tgcagcccat ggacggcccg cagcgccagg ccaataccga gtgggcggtg caatggtgcc
      541 tggaccaccc gcgctggcag ctcagcttgc agacgcacaa atatctcggc atccgttgag
      601 ccccttcatg tttgaactga gccaatcctt ctatttcgag tccgcccaca cgctgcggcg
      661 caaggtcgag cgcaacgagg ccgacggcag ccgccgcgtg catggccaca cctacaccgc
      721 cgaagtgagc gtgcgcggcc agatcgatcc cgagaccggc atggtggtgg acctcgccgt
      781 gctgcgggcg gcgatcgccg ccgtgcgggc caagctggat catcattttc tcgacgaggt
      841 gcccggcctg ggcgcgccga cgctggagaa cctttgcgtc ttcatccaca agcagctgag
      901 cgccgccgtg ccgggcgtgt gcaaggtgag cgtggagcgc aagagcagcg gcgaccgctg
      961 tagctatcgc ccggccgcat gagcgaagct tccctgccgg aacgcagcct tcgcgccgtc
     1021 tggcatccct gcacgcagat gaagcgccac gagcgcgagc ctgtgctcgc catcgtgcgc
     1081 ggtgaggggc cttggctcta tgacgaccag ggcaggcgct atctcgatgc cgtgagttcc
     1141 tggtgggtca atctcttcgg ccatcaacat gccggcatca aggcggcgct ggtcgaccag
     1201 cttgatcgca tcgagcacgt gatgctggcc gggctcacgc atgagccggt ggtcgcactg
     1261 tccgagcgcc tcgcggcgct gaccgggctt ggccatgcct tctacggcag cgatggcgcc
     1321 tcggccaccg agatcgcgct gaagatgagc gcccactact ggcgcaacct gggccggccg
     1381 ggcaaatgcg aattcatcgg cctggcgggc ggctatcacg gcgagacggt gggcgccttg
     1441 tcggtgaccg acatcgcgat ctttcgcgag gcctatgcgc cactggtgag gctgagcgcc
     1501 gcgctgccta cgcctgatgc ccggctcgcg gcgcccgggg tttctccgcg cgaacacgcc
     1561 gagcaatgcg cgcgagccct gcaggcctat ctcgaagcgc atcacgagcg cacggccgcg
     1621 ctgatcctcg agccgttggt gcagtgcgcg tccggcatgg cgatgcacga tgccgagtac
     1681 ctgcgcttgg cgcgcgcttt gtgcgaccgc taccaggtgc acctgatcgc cgacgagatc
     1741 gcggtgggct tcgggcgcac ggggacgatg ttcgcgcacc agcaggccgg catccggcct
     1801 gatttcatct gcctgtccaa ggggctcacg ggcggctatc tgccgctgtc cgctgtgttg
     1861 acgaccgata ccgtctacca ggccttctac gacgacagca cggcgcgcgg ctttttgcat
     1921 tcgcattcct acaccggcaa tcccctggcc tgccgggcgg cgctggcggt gctggacgtc
     1981 tttgagcgct cgccggtgct ggagcgcaac cgcgccgccg cggcgcgctt gaaccagctg
     2041 agcacagtgc tcgccgctca tccgcgcgtg cggcatgccc
//
LOCUS       DASUCY010000070         4454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_178241, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000070 DASUCY010000000
VERSION     DASUCY010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178241"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VFV25_01465"
     CDS             complement(<1..432)
                     /locus_tag="VFV25_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide transporter"
                     /protein_id="HEY1392011.1"
                     /translation="MNEATLTPSLGSTSGAQRWAWLPPIGSWRYHLLLAALGIFVLGP
                     LGGVTASYMNFSIGFFVGGQVLAGILGSTVTLGYGPEGKHGANYMQTAAASVAGMGGM
                     AVLVQAMVWMGLPQPPLWQLMLYMGAIGMFGAGVGMLYTPIL"
     gene            548..1354
                     /locus_tag="VFV25_01470"
     CDS             548..1354
                     /locus_tag="VFV25_01470"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY1392012.1"
                     /translation="MPLPTNAPLLSLDRVGKRYASGTLALQELTLAIVRHEFVSLLGP
                     SGCGKSTALRLLAGLDEPSSGRLQWDDGGSQASVHNDIGYVFQEPTLMPWASAADNVA
                     LPLRLRGTPRADIEAAVMPALAAVGLREFAQAYPRELSGGMRMRVSIARALVTEPALL
                     LLDEPFAALDEITRFRLNQDLLALWLREPAHAERGFTGIFVTHSVYEAVFLSQRILVM
                     APRPGRVVDEIAIDLPYPRNEATRSLPRYTELCVQVSRALQAASEVVEHA"
     gene            1347..2129
                     /locus_tag="VFV25_01475"
     CDS             1347..2129
                     /locus_tag="VFV25_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY1392013.1"
                     /translation="MPERGALALRWAAPALAVLVFLLGWEAAVRHYDIPPYLLPAPSL
                     VLRTLWDNFGSLAQSWWVTVSITFAALALAVLGGVLLAAVFALSRWVEWSLTPFAVVL
                     QVTPVIAVAPLIMILTDSALATVLVCAWIVAFFPILANTVTGLRSADANLRDLFRLYR
                     ASRWQTLRHLLIPSALPYFMTGLKIAGGLSLIGAVVAEFTAGAAGRNTGLASRILEAA
                     FRTEIPKMFAALLLVSLTGVAIYLLFALLSRLVLKRWHESEA"
     gene            2209..3225
                     /locus_tag="VFV25_01480"
     CDS             2209..3225
                     /locus_tag="VFV25_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY1392014.1"
                     /translation="MMRRILLVLALLVAGNAAQAQEKVVFATNWKAQAAHGGFYQALA
                     DGTYKRYGLDVEIRQGGPQVNNRPLLPAGRIDFLMTGNLLHSFDNVKNGVPVVVVASM
                     FQKDPQALFAHPGQGYAKFTDLVKAPVAYIAKDAEFSWWAWLKAEYGFKDEQLRPYNY
                     NLGPFLANKKSIQQGYAVEEPISIAKQGDFKPLTFLLADYGYSTYSTTIVARRETVAQ
                     HPELVRKFVEASIIGWVNYLYGDRRAADALIERDNPEMNDALIAQSIALMKELGIVDS
                     GDALKLGIGAMRPERIEDFYAKMVKAGLYKPGEVDLRQVAVYDFVNRGVGTDLKKKLA
                     ASTK"
     gene            3267..3668
                     /locus_tag="VFV25_01485"
     CDS             3267..3668
                     /locus_tag="VFV25_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3224 domain-containing protein"
                     /protein_id="HEY1392015.1"
                     /translation="MSTATGSFEVQLTPLPSSAEAEGAVVGRRSIVKQFHGDLEASSA
                     GEMLSAGSPVKGSAGYVAIEWVSGKLQGRSGSFALQHFGVMERGAGRLTVSVVPDSGS
                     GELVGLSGKLDVRIVDGQHFYDFDYSLDAAP"
     gene            complement(3693..>4454)
                     /locus_tag="VFV25_01490"
     CDS             complement(3693..>4454)
                     /locus_tag="VFV25_01490"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEY1392016.1"
                     /translation="RPATRPTAPLIPLAPRGAARNDEEAIKLIGLRRKIAQKMQEAKR
                     RIPHFSYIEEVDVTELEALRARLNAKWGAQRPRLTVLPFLVRALVLALREHPEINARF
                     DDEAGVVTRYGAAHVGIATQTAAGLMVPVLSHAETHDLWSAAAEIARLAEAARSGKIV
                     RDELSGSTITISSLGPLGGIVTTPVINHPEVAIIGVNRIVERPTMRNGAVVPRLLMNL
                     SSSFDHRVVDGMNAAEFVQALRGYLESPASLFIEA"
BASE COUNT          714 a         1498 c         1513 g          729 t
ORIGIN      
        1 caggatgggc gtgtagagca tgcccacgcc ggcgccgaac atgccgatcg cgcccatgta
       61 gagcatcagc tgccacagcg ggggctgcgg caggcccatc cacaccatgg cctgcaccag
      121 cacggccatg ccgcccatgc ccgccaccga ggcggcggcc gtctgcatgt agttggcgcc
      181 atgcttgcct tcggggccgt agcccagggt cacggtggag ccgaggatgc cggccagaac
      241 ctgtccgccg acgaagaagc cgatcgagaa gttcatgtag gaggcggtca cgccgcccag
      301 cgggccgagc acgaagatgc ccagggcggc aagcagcagg tggtagcgcc agctgccgat
      361 ggggggcagc caagcccagc gttgtgcgcc ggacgtgctg ccgagggaag gggtcagggt
      421 tgcttcattc atgggccgca accctagcct acgcgggtgg tggcgtgaat tcggagtgtc
      481 gagagtcgag gccgaagcgt cagctagttc tcagagtcgt gtgagcttga agctacagtc
      541 tgcgcccatg cctttgccca cgaacgcccc gctgctaagc ctggatcgcg tcggcaagcg
      601 ctatgccagc ggcacgctgg cgctgcagga gctgacgctg gccatcgtcc ggcacgagtt
      661 cgtcagcctg cttggcccct cgggctgcgg caagagcacg gcgctgcgcc tcttggccgg
      721 gctggatgag ccgagctccg gccgcctgca atgggacgat ggcggctcgc aagcctcggt
      781 gcacaacgac atcggctatg tcttccagga gcccacgctg atgccctggg ccagcgcggc
      841 cgacaacgtg gcgctgcccc tgaggctgcg cggcacgcct cgcgcggaca tcgaggccgc
      901 cgtgatgccg gcgctggccg ccgtgggcct gcgcgaattc gcgcaggcct atccgcgcga
      961 actctcgggc ggcatgagga tgcgcgtctc gatcgcgcgc gccctcgtca ccgagccggc
     1021 gctgctgctg ctcgacgagc ccttcgcggc gctggacgag atcacgcgct tcaggctcaa
     1081 ccaggacctg ctcgcgctgt ggctgcgcga gccggcgcat gctgagcggg gcttcacggg
     1141 catcttcgtc acgcacagcg tctacgaggc ggtgttcctg agccaacgca tcctcgtcat
     1201 ggcgccgcgc ccggggcgcg tggtcgacga aatcgcgatc gacctgccct atccgcgcaa
     1261 cgaggccacg cgcagcctgc cgcgctacac cgagctgtgc gtacaggtgt cgcgtgcgct
     1321 gcaggcggcg agcgaggtgg tcgagcatgc ctgagcgcgg cgcacttgcg ttgcgctggg
     1381 ccgcaccggc cctggccgtg ctcgtcttcc tcctgggctg ggaggccgcg gtgcggcact
     1441 acgacattcc gccctatctg ctgcccgcgc ccagcctcgt gctgcgcacg ctctgggaca
     1501 acttcggatc tctcgcgcag agctggtggg tcacggtgag catcaccttc gcggcgctcg
     1561 cgctcgcggt gctgggcggc gtgctgctgg cggccgtgtt cgcgctgtcg cgctgggtgg
     1621 agtggagcct cacgcccttc gccgtcgtgc tgcaggtcac gccggtgatc gcggtcgcgc
     1681 cgctgatcat gatcctgacc gacagcgcgc tcgccacggt gctggtctgc gcctggatcg
     1741 tcgccttctt tcccatcctc gccaacactg tgaccgggct gcgcagtgca gatgcgaacc
     1801 tgcgcgacct gtttcgcctg tatcgcgcca gccgctggca gacgctgcgc catctgctga
     1861 tcccgagcgc cctgccctat ttcatgaccg gcctcaagat cgctggaggc ctgagcctga
     1921 tcggcgccgt ggtggccgag ttcaccgccg gcgcggccgg gcgcaacacc ggcctggcct
     1981 cgcgcatcct cgaagcggcc ttccgcaccg agatcccgaa gatgttcgcg gcgctgctgc
     2041 tggtatcgct gaccggcgtg gcaatctatc tgctgttcgc gctgctgtcg cggctggtgc
     2101 tcaagcgctg gcacgagagc gaggcctagc aacggcgttg atgacgcggc acgatcgccg
     2161 cctatcatcg ccctccggct tccgttcaac agggaaaggc atgacccgat gatgcgaaga
     2221 atcttgttgg tgctggcatt gctcgtcgca ggtaatgcag cccaggccca ggaaaaagtc
     2281 gtcttcgcca ccaactggaa ggcccaggcc gcgcacggtg gcttctatca ggcgctggcc
     2341 gacggcacct acaagcgcta tggcctggat gtggagatcc gccagggcgg gccgcaggtc
     2401 aacaaccggc cgctgctgcc ggccggccga atcgacttcc tgatgaccgg caacctgctg
     2461 cacagcttcg acaacgtgaa gaacggcgtg ccggtggtgg tcgtggcctc gatgttccag
     2521 aaggacccgc aggcgctgtt cgcgcatccc ggccagggct atgcgaagtt caccgacctc
     2581 gtgaaggcgc ccgtcgccta catcgccaag gacgcggagt tctcatggtg ggcctggctc
     2641 aaggccgaat acggcttcaa ggacgagcag ctgcggccct acaactacaa cctcggcccc
     2701 ttcctggcca acaagaagag catccagcag ggctatgcgg tcgaggagcc gatcagcatc
     2761 gccaagcagg gcgacttcaa gcccctgact ttcctgctgg ccgactacgg ctactcgact
     2821 tattcgacga ccatcgtggc gcgccgcgag acggtggccc agcaccccga gctggtacgc
     2881 aagttcgtcg aggcctcgat catcggctgg gtcaactatc tctacggcga tcgccgcgcc
     2941 gccgacgcgc tgatcgagcg cgacaacccc gaaatgaacg acgcgctgat cgcgcagtcg
     3001 atcgccctga tgaaggagct gggcatcgtc gattcgggcg atgcgctcaa gctcggcatc
     3061 ggcgcgatga ggcccgagcg catcgaggac ttctacgcca agatggtcaa ggcggggctc
     3121 tataagccgg gtgaggtcga cctcaggcag gtcgcggtct acgacttcgt gaaccgcggc
     3181 gtcggcaccg acttgaaaaa gaagctcgcc gcctcgacca agtgacgcgc catatcgatc
     3241 ctatgacaag caagggagcc ggcaccatga gcacagccac cggcagcttc gaagtccagc
     3301 tcacgcccct gccctcgagc gcggaagccg aaggcgccgt ggtgggccgc cgttccatcg
     3361 tcaagcaatt ccatggcgat ctggaggcga gcagcgccgg cgagatgctc agcgctggtt
     3421 cgcccgtcaa gggctcggcc ggctatgtgg cgatcgaatg ggtcagcggc aagctgcagg
     3481 ggcgcagcgg cagcttcgcg ctccagcatt tcggcgtgat ggagcgcggc gcgggacgat
     3541 tgaccgtgag cgtcgtgccg gactcaggca gcggcgagct cgtgggcctg tccggcaagc
     3601 tggacgtccg catcgtcgac ggacagcatt tctacgactt cgactacagc ctggacgcgg
     3661 cgccctgaga gtcgcgtcgg cgagcctgcc cgctacgctt cgatgaacag cgaggcaggg
     3721 ctctccagat aaccgcgcag cgcctgcacg aactccgccg cgttcatgcc gtcgaccacg
     3781 cggtggtcga aggacgacga gagattcatc agcagccgcg gcaccacggc gccgttgcgc
     3841 atggtcgggc gctcgacgat gcggttgacg ccgatgatgg cgacctcggg gtggttgatc
     3901 accggcgtcg tgacgatgcc gcccagcggc cccaggctgc tgatcgtgat ggtcgagccc
     3961 gagagctcgt cgcgcacgat cttgccgctg cgcgccgcct cggccaggcg cgcgatctcg
     4021 gccgcggccg accacagatc gtgagtctcg gcatggctca gcaccggcac catcagtccg
     4081 gccgcggtct gcgtcgcgat gccgacgtgg gccgcgccat agcgcgtgac gacaccggcc
     4141 tcgtcatcga agcgggcatt gatttccggg tgctcgcgca aggccagcac caaggcgcgg
     4201 accaggaagg gcagcacggt caggcgcggg cgctgtgcac cccatttggc attgagcctg
     4261 gcgcgcagcg cctcgagctc ggtgacgtcg acctcctcga tatagctgaa atgcgggatg
     4321 cgccgcttgg cctcctgcat cttctgcgcg atcttgcgac gcaggccgat cagcttgatc
     4381 gcctcttcgt cgtttcgcgc cgcgccgcgc ggggcgagtg ggataagcgg ggccgtgggc
     4441 cgggtcgccg ggcg
//
LOCUS       DASUCY010000071         2207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_403087, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000071 DASUCY010000000
VERSION     DASUCY010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403087"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2207)
                     /locus_tag="VFV25_01495"
     CDS             complement(<1..>2207)
                     /locus_tag="VFV25_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY1392017.1"
                     /translation="DGKQQSFAVYTNDQILQADPWNDMVLAYRNGAPVRVRDIGVALE
                     GPENVRTAAWAYAGAAAPADSTVRNGRGMTLFITKQPGANVIATVERIRAALPKLQAA
                     IPPSVAVSTLVDRTQNIRASVHEVEITLLITIAAVIMVISVFLLSVPATVIPGLTVPL
                     ALTGTAAVMYLMGYSLDNLSLMALTISVGFVVDDAIVMLENIYRHVEQGTKPMEAAYK
                     GAAEIGFTIISISVSLVAVFIPLLLMGGIVGRLFREFAVTVTLAIAVSVFISLTLTPM
                     LCSRYLSDTHAKQHGRLFLLFERGFDAMLAGYKRGLDFVLKHEFATLCVFFATLTLTV
                     AMFVLSPKGFFPQQDTGTIYGFAETSQDASFVAMQQRMTQAADVVRQDPDVTAFGMFA
                     GGSTANTGAFFIALKPRDEGRNATADEVITRLRPKLANLEGINLFMQAPQDINVGGRL
                     SRTQYQYTLTDTNLDELNTWAPRLLERLRREPVLTDLATDQQNAAAAAVLTIDRARAA
                     SFGSPPAQIDATLYDAIGQRQVAQYFTQTNSYHVVLEVTPQLQQDPALFDKLYVASPQ
                     NGRQVPLSTFVKVDTSKTAYLQISHQGQFPAVTISFNLAPGVALGDAVKAIEKVQAEM
                     NVPATLSGSFQGTAKAFGESLASQPYLIAAALVAVYIVLGLLYESYIHPLTILSTLPS
                     AGLGALLILRLGGYDLSVIALIGIILLIGIVKKNGIMMIDFALSAERERGLDA"
BASE COUNT          363 a          714 c          768 g          362 t
ORIGIN      
        1 gcgcgtcgag gccgcgctcg cgctcggcgc tgagcgcaaa gtcgatcatc atgatgccgt
       61 tcttcttgac gatgccgatc agcaggatga tgccgatcag cgcgatcacg ctcaggtcgt
      121 agccgcccag gcgcaggatc agcaaggcgc ccaggccggc cgagggcagc gtcgagagga
      181 tcgtcagtgg gtggatgtag ctctcgtaga gcaggcccag cacgatgtag acggccacca
      241 gcgcggcggc gatcagatag ggctgcgagg ccagcgattc gccaaaggcc ttggccgtgc
      301 cctggaaact gccggacagc gtcgccggca cgttcatctc ggcctgcacc ttctcgatcg
      361 ccttcacggc atcgcccagg gcgacgcccg gcgcgaggtt gaaggagatc gtcaccgccg
      421 ggaactggcc ctggtggctg atctgcaggt aggcggtctt gctggtgtcg accttcacga
      481 aggtcgacag cggcacttgg cgcccgttct gcggagaggc gacatagagc ttgtcgaaca
      541 gcgccggatc ctgctgcagc tgcggcgtaa cctccagcac cacgtgatag ctgttggtct
      601 gggtgaagta ctgcgcgacc tggcgctggc cgatcgcgtc gtacagcgtc gcatcgatct
      661 gcgccggcgg gctgccgaag ctcgcggcac gcgcgcggtc tatcgtcagc accgccgcgg
      721 cggcagcgtt ctgctggtcg gtcgcgaggt cggtcagcac gggctcgcgg cgcagccgct
      781 ccagcaggcg tggtgcccag gtgttgagct cgtcgaggtt ggtgtcggtc agcgtgtact
      841 gatactgggt gcgcgacagc cggccgccga cgttgatgtc ctgcggcgcc tgcataaaga
      901 ggttgatgcc ctccaggttc gcgagcttgg ggcgcagcct ggtgatcacc tcgtcggcgg
      961 tggcgttgcg gccctcgtcc ctgggcttga gcgcaatgaa aaaggccccg gtgttggccg
     1021 tgctgccgcc ggcgaacatg ccgaaggcgg tcacgtcggg gtcctggcgc accacgtcgg
     1081 ccgcctgggt catgcgctgc tgcatcgcga cgaaggaggc gtcctgcgaa gtctcggcga
     1141 agccgtagat ggtgccggtg tcctgctgcg ggaagaagcc cttggggctc agcacgaaca
     1201 tcgccaccgt cagcgtcagc gtcgcgaaga acacgcacag cgtcgcgaac tcgtgcttca
     1261 gcacgaagtc caggccgcgc ttgtagcccg ccagcatcgc gtcgaagccg cgctcgaaga
     1321 gcaggaagag gcggccgtgc tgcttggcat gggtgtcgct caggtagcgc gagcacagca
     1381 tgggcgtgag cgtcagcgag atgaacaccg acacggcgat ggccagcgtc accgtcaccg
     1441 cgaactcgcg gaacagccgg ccgacgatgc cgcccatcag cagcagcggg atgaagaccg
     1501 ccaccagcga gaccgagatc gagatgatgg tgaagccgat ctcggccgcg cccttgtagg
     1561 ccgcctccat cggcttcgtg ccctgctcga cgtgccggta gatgttctcc agcatcacga
     1621 tcgcatcgtc gacgacgaag cccaccgaga tcgtcagcgc catcagcgac aggttgtcca
     1681 ggctgtagcc catcaggtac atcacggcgg ccgtgcccgt cagggccagc ggcacggtga
     1741 ggccggggat cacggtggcc ggcacgctga gcaggaagac cgagatcacc atgatcacgg
     1801 ccgcgatcgt gatcagcagc gtgatctcca cctcgtgcac cgaggcgcgg atgttctgcg
     1861 tgcggtcgac cagcgtgctg accgcgaccg agggcgggat cgccgcctgc aacttgggca
     1921 gcgccgcgcg tatgcgctcg accgtcgcga tcacattggc gcccggctgc ttggtgatga
     1981 acagcgtcat gccgcgcccg ttgcgcacgg tgctgtcggc cggggcggcg gcgccggcat
     2041 aggcccaggc cgcagtcctg acgttctccg gcccttccaa cgcgacgccg atgtcgcgca
     2101 cccgcaccgg cgctccgttg cgataggcca gcaccatgtc gttccacggg tcggcctgca
     2161 ggatctggtc gttggtatag accgcgaagc tctgctgctt gccgtcg
//
LOCUS       DASUCY010000072         5661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_419171, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000072 DASUCY010000000
VERSION     DASUCY010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 5661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5661
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419171"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1223
                     /locus_tag="VFV25_01500"
     CDS             <1..1223
                     /locus_tag="VFV25_01500"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HEY1392018.1"
                     /translation="KVLVKPGWLAVYGKEAQEDDANLVPVQPNETVRTESVDVTALKT
                     KPPARYTEATLLSAMEGAGKLIDDDDLREAMQEKGLGTPATRAAIIEGLILEKYMVRE
                     GRELIPTAKAFQLTTLLRGLDVEDLTKPELTGNWEYQLSQMEKGKLSREAFMNEIAVM
                     TRRVVQKAKEYDRDTIPGDYATLKTPCPNCGGTVKENYRRFACDGGGTAPGGASCDFS
                     ISKIPGGRSFELDEVESFLAHKKIGPLEGFRSKAGWPFTAELKLAYDEDIKNWKLEFD
                     FGDDAKKEGESGEPVDFSGQESLGACPKCKGHIYEHGTGYVCEHSVGAHVTCDFKSGK
                     IILQQPIAREQMSKLLLTGKTDLLENFVSNKTRRKFKAYLAFDQKEGKVVFEFEPRAA
                     AKKAPAKKAAAKKA"
     gene            1254..1673
                     /locus_tag="VFV25_01505"
     CDS             1254..1673
                     /locus_tag="VFV25_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392019.1"
                     /translation="MSMLACGVLASHAGASPEGGVAADVDSLAAASYFATGPTGVGGV
                     EPPMLALMQRLHREPSARELMGQAFARGSLEARLYVACWAESYAREDYPRYKATLLAA
                     DGQASTMTGSVLRKEATAEVLARIDKGGCGRLLVGAS"
     gene            1745..2302
                     /locus_tag="VFV25_01510"
     CDS             1745..2302
                     /locus_tag="VFV25_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEY1392020.1"
                     /translation="MPLVSRQGAIGALLIAAASTAVAGSFQLTSSTIKPNAKLAEAQV
                     FDGFGCSGKNISPALKWSGAPAGTRSYAVTVYDPDAPGSGWWHWVVYNIPGSASEIPQ
                     GAGGAGGKGLPSGSVQGRTDFGAPGYGGACPPQGDKPHRYIFTVHALKVDKLDVSPDS
                     PPAQVGRMIDANRLDKASLTATYSR"
     gene            2367..3350
                     /locus_tag="VFV25_01515"
     CDS             2367..3350
                     /locus_tag="VFV25_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEY1392021.1"
                     /translation="MSALILLKLLAIFVVVVIGWLAGRTRFFGGGVAARALSSTAFYL
                     FAPCLLFRTTARIDFAAMPWSTLVAYFAPVVAWLLAIYAWQRLGARHEADPAPAEPSV
                     RAITATFGNTAQLGIPVAAALFGEAGLSIHLAIVSLHALTLLSVITVLVELDVAHAAA
                     RRSERRASLSATLALTARNTVIHPVVLPVLMGLAWNLTGIAIPRPVDEILLMLGQAVV
                     PVCLIAIGMSLAHFGLQGSLRNAFKLTVAKLAVQPALVLGVGHYGFGLGGMPLAVIVT
                     CASLPAGTNSLMFSQRYDTLEAETTATLVLSTLAFVLAAPAWLWLTSHLAP"
     gene            3413..4693
                     /locus_tag="VFV25_01520"
     CDS             3413..4693
                     /locus_tag="VFV25_01520"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEY1392022.1"
                     /translation="MTAADLQATMDHLGAAARAASSAMAAAFTAAKNAALLTLAHEVR
                     AQRPALQAANAQDVSAAEANGLAAPMVDRLRLSDKVVEQMAEGCEQVAALPDPIGELI
                     NFKRRPSGITVGQMRVPLGVFGMVYESRPNVTIDAASLAIKSGNAAILRGGSEAIHSN
                     LALWRLVQAALVEASLPPDAVQLVQTTDRAAVGRLIAMPEYVDVIIPRGGKSLIERIS
                     REATVPVIKHLDGNCHVYVDAEVDIELAVKVTDNAKTQKYSPCNAAESLLVHEAQAAA
                     FLPKIGTIFAAKGVEMRCDAAAKAVLQAVPSAKLADANEQDWSEEYLAPVISVKLVKS
                     LDEAIAHINRYGSHHTDAILTTNHPNAMRFLREVDSASVMVNASTRFADGFEYGLGAE
                     IGISTDKFHARGPVGLEGLTSLKWVVFGQGEVRV"
     gene            4782..5486
                     /gene="queC"
                     /locus_tag="VFV25_01525"
     CDS             4782..5486
                     /gene="queC"
                     /locus_tag="VFV25_01525"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEY1392023.1"
                     /translation="MSDPRTAIVLFSGGQDSTACLAWALDRYARVETVGFDYGQRHRI
                     ELDCRPRVISALRAAFPQWSAKLGEDHVLDLALLGQISDTALTEARAIEMQANGLPNT
                     FVPGRNLLFFTFAATLAYRRGASVLVGGMCETDYSGYPDCRDNTLKALQIALSLGMDT
                     PMTVETPLMWLTKAQTWSLTERLGGAPLNELIAEHTHTCYLGERSVRHPWGYGCGKCP
                     ACELRRAGYESWLAQQ"
     gene            complement(5500..5649)
                     /locus_tag="VFV25_01530"
     CDS             complement(5500..5649)
                     /locus_tag="VFV25_01530"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY1392024.1"
                     /translation="MLTCCEFGRISTLAAATLHSLGFQRAIALDGGLQAWREAGYPLK
                     SGSAP"
BASE COUNT          939 a         1928 c         1940 g          854 t
ORIGIN      
        1 gcaaggtgct cgtcaagccg ggctggctcg cggtctacgg caaggaggcg caggaggacg
       61 acgccaacct ggtgccggtg cagccgaacg agaccgtgcg caccgagagc gtcgacgtca
      121 cggcgctcaa gaccaagccg cccgcgcgct acacggaagc gacgctgctg tccgcgatgg
      181 aaggcgccgg caagctgatc gacgacgacg atctgcgcga ggcgatgcag gagaaaggcc
      241 tgggtacgcc ggccacgcgc gcggccatca tcgaaggcct gattctcgag aaatacatgg
      301 tgcgcgaggg ccgcgagctg attcccacgg ccaaggcctt ccagctgacg acgctgctgc
      361 gcgggctgga cgtggaggac ctgaccaagc ccgagctcac aggcaactgg gaataccagc
      421 tgtcgcagat ggagaagggc aagctcagcc gcgaagcctt catgaacgaa atcgcggtga
      481 tgacccgccg cgtcgtgcag aaggccaagg aatacgatcg cgacaccatc cccggcgact
      541 atgcgacgct gaagacgcct tgccccaact gcggcggcac tgtgaaggag aactaccgcc
      601 gcttcgcctg cgatggcggc ggaactgcgc ctggcggcgc ctcgtgcgac ttctcgatca
      661 gcaagatccc cggcggacgc agcttcgagc tggacgaggt cgagagcttc ctggcccaca
      721 agaagatcgg cccgttggag ggatttcgtt ccaaggcggg ctggcccttc acggccgagc
      781 tcaagctcgc ctatgacgag gacatcaaga actggaagct ggaattcgac ttcggcgacg
      841 acgcgaagaa ggaaggcgag tccggcgagc cggtggactt ttcaggccag gagagcctgg
      901 gcgcctgccc caagtgcaag ggccatatct acgagcatgg caccggctat gtctgcgagc
      961 acagcgtcgg cgcgcacgtg acttgcgatt tcaagagcgg caagatcatc ctgcagcagc
     1021 ccatcgcgcg cgagcagatg agcaagctgc tgctaacggg caagaccgat ctgctggaga
     1081 acttcgtctc caacaagacg cggcgcaagt tcaaggccta tctggccttc gaccagaagg
     1141 aaggcaaggt cgtcttcgag ttcgagccgc gcgcagcggc caagaaggcg cccgccaaga
     1201 aggctgccgc gaagaaggcc tgaaagggga gtgaaatgca aggaagacgc ttgatgtcga
     1261 tgctcgcctg cggcgtgctg gcaagccatg caggcgcctc gccagaaggc ggcgtggctg
     1321 ccgacgtgga cagcctcgcg gcggccagtt atttcgcgac cggccccacc ggcgtcggcg
     1381 gtgtggagcc gccgatgctg gccttgatgc agcggctgca ccgcgagccc agcgcccgcg
     1441 agctgatggg ccaggccttc gcgcgcggca gcctcgaggc gcgcctgtac gtggcctgct
     1501 gggccgaaag ctatgcccgc gaggactatc cgcgctacaa ggccacgctg ctggccgcgg
     1561 acgggcaggc cagcacgatg acgggctcag tgctgcgcaa ggaggccacg gccgaggtgc
     1621 tggcgcgcat cgacaagggc ggctgcgggc gcttgctggt gggcgcgagc tagcttctgc
     1681 tcgcgcgtgc ctgcgcggcg cgcaaggcgc atgatgaatg ccttcttcgc tgaaggagat
     1741 cgccatgccc ctcgtctcgc gccagggcgc tatcggcgcc ttgctcatcg ccgccgcctc
     1801 cactgccgtg gccggcagct tccagctgac cagcagcacc atcaagccca atgccaagct
     1861 cgccgaggcc caggtcttcg acggcttcgg ctgcagcggc aagaacatct cgccagccct
     1921 gaaatggagc ggcgcgccgg ccggcaccag gagctacgcg gtcacggtct atgaccccga
     1981 cgcgcccggc tcgggctggt ggcactgggt ggtctacaac atcccgggca gcgcaagcga
     2041 aatcccgcag ggcgcaggtg gcgccggcgg caagggcctg ccttctggca gcgtccaggg
     2101 ccgcaccgat ttcggcgcgc cgggctatgg cggcgcctgc ccgccgcagg gcgacaagcc
     2161 gcaccgctac atcttcacgg tgcatgcctt gaaggtcgac aagctcgatg tgtcgcccga
     2221 ttcgccaccc gcgcaggtcg gccgcatgat cgacgcgaac cgcctggaca aggccagcct
     2281 caccgcgacc tactcgcgct gagtcacgct gagcgcggcg ggcatcgcgc ggcgggtgct
     2341 tcctcgcgct gggcatactg cggcgcatgt ccgcattgat tctgctcaag ctgctggcga
     2401 tcttcgtggt cgtcgtgatc ggctggctgg ccggccgcac gcgtttcttt ggcggcggcg
     2461 tcgctgcgcg tgccttgtcg agcaccgcct tctacctgtt cgcgccctgc ctgctgttcc
     2521 gcaccaccgc gcgcatcgac ttcgcggcca tgccctggtc gacgctggtg gcctatttcg
     2581 cgccggtcgt ggcctggctg ctggcgatct acgcctggca gcggctgggc gcgcgacacg
     2641 aggccgatcc ggcgccggcc gagcccagcg tgcgcgccat caccgccacc ttcggcaata
     2701 cggcgcagct gggcattccc gttgcggcgg ctttgttcgg ggaggccggg ctgtccatcc
     2761 acctcgccat cgtgagcctg cacgcgctca cgctgctgag cgtgatcacg gtgctggtgg
     2821 agctggacgt ggcgcacgcc gcggcgcggc gctccgagcg ccgtgcgagc ctgagcgcca
     2881 ccttggcgct caccgcccgc aacaccgtta tccatccggt ggtgctgccg gtgctgatgg
     2941 gcctcgcgtg gaacctcacc ggcatcgcga tcccgcgccc ggtggatgaa atcctgctga
     3001 tgctgggcca agcggtggtg ccggtctgcc tcatcgccat cggcatgtcg ctggcgcact
     3061 tcggcttgca ggggtcgctg cgcaacgcgt tcaagctcac ggtggcgaag ctggctgtgc
     3121 agccggcgct ggtgctgggc gtcgggcact atggcttcgg cctcggcggc atgccgctcg
     3181 cggtgatcgt cacgtgcgcc agcctgcccg ccggcaccaa ctcgctgatg ttctcgcagc
     3241 gctacgacac gctggaggcc gagaccacgg cgaccctcgt cctgtcgacc ctggccttcg
     3301 tgttggccgc accggcctgg ctgtggctga cgagccatct cgcgccctag ctgtgtatcg
     3361 gcgaaaagag cccaccgccg gcgctggcgg gacggtttgc gacaattgcg gcatgaccgc
     3421 tgccgacttg caggccacga tggaccacct gggcgctgcc gcccgggccg catccagcgc
     3481 gatggccgct gccttcaccg ccgccaagaa cgcggccctg ctcaccctcg cccacgaggt
     3541 ccgcgcgcag cgaccggcac tgcaggcggc caatgcgcag gacgtgagcg cggccgaagc
     3601 gaacggcctg gccgcgccca tggtcgaccg gctgcgcctg agcgacaagg tggtcgagca
     3661 gatggccgag ggctgcgagc aggtggccgc gctgccggat ccgatcggcg agctgatcaa
     3721 cttcaagcgc cgccccagcg gcatcaccgt gggccagatg cgcgtgccgc tgggcgtgtt
     3781 cggcatggtc tacgagagcc gccctaacgt caccatcgat gccgcgtcgc tggcgatcaa
     3841 gagcggcaat gcggccatcc tgcgcggcgg ctccgaggcc atccattcca acctcgcgct
     3901 gtggcgcctg gtgcaggccg cgctggtcga ggccagcctc ccgcccgatg cagtgcagct
     3961 ggtgcagacc accgaccgcg ctgcggtggg ccgcttgatc gcgatgcccg agtacgtgga
     4021 cgtgatcatt ccgcgcggcg gcaagagcct catcgagcgc atcagccgcg aggccacggt
     4081 gcctgtgatc aagcacctgg acggcaactg ccacgtgtat gtggatgcag aggtcgacat
     4141 cgagctcgcc gtcaaggtga ccgacaacgc caagacgcag aagtacagcc cctgcaatgc
     4201 ggccgaatcg ctgctggtgc acgaggcgca agccgcggcc ttcctgccga agatcggcac
     4261 gatcttcgcg gccaagggcg tggagatgcg ctgcgacgcc gccgcaaagg ccgtgctgca
     4321 ggccgtaccg agcgcgaagc tggcggatgc gaacgagcag gactggagcg aggaatacct
     4381 ggctcccgtc atcagcgtca agctcgtgaa gagcctcgac gaagccatcg cccatatcaa
     4441 ccgctacggc tcgcaccaca ccgacgccat cctcaccacc aaccacccga acgcgatgcg
     4501 cttcctgcgc gaggtcgact cggccagcgt gatggtcaat gccagcacgc gcttcgccga
     4561 cggcttcgag tacggcctgg gcgccgagat cggcatcagc accgacaagt tccacgctcg
     4621 cgggccggtg gggctggagg ggctgacgtc gctgaagtgg gtggtgttcg ggcaggggga
     4681 agtcagggta tgacgacacc ccgctcgccg aatacgtcga gcacccctcc gaaaggggcc
     4741 ggccttgctt ggggcggccc ggcgcggcgg cccgtgagcc catgagcgac ccccgcaccg
     4801 ccatcgtcct ctttagcggc ggccaggact ccaccgcctg cctggcctgg gcgttggacc
     4861 gctatgcgcg cgtcgagacc gtcggcttcg actatggcca gcgccaccgc atcgagctcg
     4921 actgccggcc gcgcgtgatc tcggcgttgc gtgcggcctt cccgcaatgg agcgcgaagt
     4981 tgggcgagga ccatgtgctc gaccttgcgc tgctcggcca gatcagcgac accgcattga
     5041 ccgaggcgcg cgccatcgag atgcaggcca acggcctgcc caacaccttc gtgccggggc
     5101 gcaacctgct cttcttcacc ttcgccgcga cgctggccta tcgccgcggc gcctctgtgc
     5161 tggtgggcgg catgtgcgag accgactatt ccggctaccc cgattgccgc gacaacacgc
     5221 tgaaggcgct gcagatcgcg ctgagcctgg gcatggacac gccgatgacg gtggagacgc
     5281 cgctgatgtg gctgaccaag gcacagacct ggtccctgac cgagcggctc ggcggtgcgc
     5341 cgcttaacga gctgatcgcc gagcacacgc acacctgcta tctcggcgag cgcagcgttc
     5401 gccatccctg gggctatggc tgcggcaaat gcccggcctg cgagctcagg cgcgcaggtt
     5461 acgagagctg gctggcgcag cagtgagatc cgccgtccgt cacggcgcgc tgccggactt
     5521 gagcggatag cccgcttcgc gccaggcctg caggccgccg tccagcgcaa tcgcgcgctg
     5581 gaagccgagc gagtgcagag tcgccgcggc gagcgtggag atgcggccga actcgcagca
     5641 ggtgaggatg cgccgcgtcg g
//
LOCUS       DASUCY010000073         2689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_49726, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000073 DASUCY010000000
VERSION     DASUCY010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49726"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VFV25_01535"
     CDS             <1..204
                     /locus_tag="VFV25_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392025.1"
                     /translation="GYEKLMRQWLAAAPAGTVLMAHPATRLVDGDAIAAARVAEFSYL
                     SGPAFGDLLSQLGVRPTRGSELF"
     gene            258..1736
                     /locus_tag="VFV25_01540"
     CDS             258..1736
                     /locus_tag="VFV25_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEY1392026.1"
                     /translation="MSASQQPSSLNTQALQSFVERAWDEQIVPELTQYIEIPAKSPMF
                     DAQWESNGHIATVARRAASWVEEQKVAGLKLELIKLPGRTPVIFFEVPATRPGGNERG
                     ADTVVMYGHLDKQPEFSGWRNDLGPWTPKYEGGKLYGRGGADDGYAVYASISAIKALD
                     DQGIARPRIIGLIETCEESGSGDLPHYIDALRERLGNVALVVCLDSGAGNYDQLWLSS
                     SLRGNITGVLKVEILTEGVHSGDASGLVPSSFRILRQVLDRLEDSKTGQLLPASFHCE
                     IPAGRVAQAKATAAILGDEVWKRFPWACGAEGALALPTTSDPVEALLNRTWRPTLSIT
                     GVDGLPPLSNAGNVLRPYTAFKLSLRLPPLVDANRAAQDLKTLLEDNAPYHAKVTFVP
                     DGRADDQGASGWNAPALTPWLETALDQASHNHWDAPLAYMGMGGTIPLMNMLQKGFPK
                     AQMMVCGVLGPKSNAHGPNEFLHVPYGKRLTAAVAEVIAAMP"
     gene            complement(1747..2517)
                     /locus_tag="VFV25_01545"
     CDS             complement(1747..2517)
                     /locus_tag="VFV25_01545"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEY1392027.1"
                     /translation="MTLAIVAAMHEELRALLPALQRQRVVLHAGREFHCGRLDGQQVV
                     LVLSRIGKVAAATTAALLIERFGVRRLLFTGVAGGLGAGVKVGDVVVARELLQHDMDA
                     SPLFARYEVPLTGRARFATDAVMSDALLLAAREGVAKVMDLTGPLSALTELGVATPQV
                     HEGLVLSGDRFVATEAEGEILRSNLSDALAVEMEGAAVAQVCHDCDIPFAVLRSISDR
                     ADDSAHVDFGRFVAEVASRYSATIVRSWLRQQPGGSAR"
BASE COUNT          449 a          929 c          918 g          393 t
ORIGIN      
        1 ggatatgaaa agctgatgcg gcagtggctg gcggcggctc cggccgggac ggtgctgatg
       61 gcgcatccgg ccacccgttt ggtcgacggc gatgccatcg cggcagcgcg cgtcgcggag
      121 ttttcctatc tgtccggacc cgccttcggc gatctgctgt cgcagctggg agtgcggccc
      181 acccgaggca gcgaattgtt ttagacgccc accagggcgg accaccggca ctaccgtgcc
      241 agcaaggagt tgatccaatg agcgcgagcc agcagccatc cagcctgaac acccaggccc
      301 tgcaatcctt cgtcgagcgg gcctgggacg agcagatcgt gcccgaactg acccagtaca
      361 tcgagatccc ggccaagagc ccgatgttcg atgcgcaatg ggaaagcaat ggccacatcg
      421 ccaccgttgc gcgccgcgcc gcgagctggg tggaagaaca gaaagtggcc gggctcaagc
      481 tcgagctgat caagctgccc gggcgcacgc cggtgatctt cttcgaggtg ccggccaccc
      541 ggccgggcgg caacgagcgc ggcgccgata cggtcgtgat gtatggccac ctggacaagc
      601 agcctgagtt cagtggctgg cgcaacgacc tcggcccatg gacgccgaag tacgagggcg
      661 gcaagctcta tggccgcggc ggcgcggacg acggctatgc ggtttatgcc tcgatctcgg
      721 caatcaaggc gctggatgac cagggcatcg cgcgtccgcg catcatcggc ctgatcgaga
      781 cgtgcgagga aagcggttcg ggcgatctgc cgcattacat cgatgcgctg cgcgagcggc
      841 tgggcaatgt ggccctggtg gtctgcctgg attcgggcgc tggcaactac gaccagttgt
      901 ggctcagcag cagcctgcgc ggcaacatta ccggcgtgct caaggtggag atcctgaccg
      961 agggcgtgca ttcgggcgac gcgagcggcc tcgtgccctc gagcttccgc atcctgcgcc
     1021 aggtgctgga ccggctggaa gacagcaaga ccggccagct gctgccggcc agcttccatt
     1081 gcgagatccc ggccgggcgt gtggcccagg ccaaggcgac cgcggccatc ctcggcgacg
     1141 aggtgtggaa gcgcttcccc tgggcctgcg gcgccgaggg cgcgctggcc ctgcccacca
     1201 ccagcgatcc ggtggaggcg ctgctgaacc gcacctggcg ccccacgctg agcatcaccg
     1261 gcgtcgacgg gctgccgccg ctgtcgaacg ccggcaacgt gctgcgcccc tacaccgcct
     1321 tcaagctctc gctgcgcctg ccgccgctgg tggatgccaa ccgcgcggcc caggatctca
     1381 agaccctgct cgaagacaac gcgccctacc acgccaaggt gaccttcgtg ccggacggcc
     1441 gcgccgacga ccagggcgcc agcggctgga atgcgccggc cctgacgcct tggctggaga
     1501 cggcgctgga tcaggcctcg cacaaccact gggacgctcc gctcgcctac atgggcatgg
     1561 gcggcacgat cccgctgatg aacatgctgc agaagggctt cccgaaggct cagatgatgg
     1621 tctgcggcgt gctgggcccc aagagcaacg cccacgggcc caatgaattc ctgcacgtgc
     1681 cctatggcaa gcggctgacc gcagccgtgg ccgaggtcat cgcggcgatg ccctgagcca
     1741 ggtgggctag cgagcggagc cgcccggctg ctggcgcagc cagctgcgca caatggtcgc
     1801 gctgtagcgg ctcgcaacct cggcgacgaa gcgcccgaaa tccacatggg cgctgtcgtc
     1861 ggcgcggtcc gagatgctgc gcagcaccgc aaaggggatg tcgcagtcgt ggcagacctg
     1921 ggccaccgcg gcgccctcca tctccaccgc cagcgcgtcg ctcagattgc tgcgcaggat
     1981 ttcgccttcg gcctcggtgg cgacgaagcg atcaccgctc agcaccaggc cctcgtgcac
     2041 ctgcggtgtg gccacgccca gctcggtgag cgcgctgagc ggtccggtga ggtccatgac
     2101 cttggcgacg ccttcgcggg cggcgagcag cagggcatcg ctcatcaccg catcagtggc
     2161 gaagcgggcg cggccagtca gcggcacttc gtagcgcgcg aacagcggcg aggcatccat
     2221 gtcgtgctgc agcagctcgc gcgcgacgac cacgtcgccc accttgaccc cggcgccgag
     2281 gccgccggcc acgccggtga acagcagccg ccgcacgccg aagcgctcga tcagcagcgc
     2341 cgccgtggtc gcggccgcca ccttgccgat gcgcgacagc accagcacga cctgctggcc
     2401 gtccagccgc ccgcaatgga attcgcgccc ggcatgcagc accacacgct ggcgctgcag
     2461 cgccggcagc agcgcgcgca actcctcgtg catggcggcg acgatggcga gcgtcatggc
     2521 atgggctgaa acaatgcggc ccgatgttgc catcgggccg tagcatggac actcagtttg
     2581 cgcgcgggac gttcgccgcg caagagctca cttgccccgg agttcgcgcc tgagaatctt
     2641 cccgaccggc gtcttgggca gctcggtgcg gaattcgacg atcttgggc
//
LOCUS       DASUCY010000074         2726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_644141, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000074 DASUCY010000000
VERSION     DASUCY010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644141"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..426
                     /locus_tag="VFV25_01550"
     CDS             82..426
                     /locus_tag="VFV25_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEY1392028.1"
                     /translation="MNKAELIDLIASSHEFSKAEAGRVLGTVLDAVVTTVKKGGSVTL
                     PGFGSFKQVSRAARSGRNPATGDKIKIAAAKLPKFTAGAAFKAAVDPKAAARKAEKAA
                     GKKPAKKAGKKA"
     gene            complement(506..1351)
                     /locus_tag="VFV25_01555"
     CDS             complement(506..1351)
                     /locus_tag="VFV25_01555"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006481242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY1392029.1"
                     /translation="MTIAITGATGQLGRLVVSQLKATLPPGQLIALVRSPAKAADLGI
                     PAREADYARPETLPAALAGVDTLLLISSSEIGQRAAQHRNVIEAAKKAGVKRIVYTSL
                     LHADTSPLSLAQEHLDTEAALKASGLAFTILRNGWYTENYTGSIPGALAGGAFIGSAG
                     QGRISSAARADYAAAAAAVLTSDGHEGKTYELAGDEAYTLGDLAAEVSRQTGKAFPFR
                     NLPEADYATALTGFGLPAGIAQAIASWDVGASNGALFDDGRQLARLIGRPTKPLAAVV
                     AAALR"
     gene            1421..1864
                     /locus_tag="VFV25_01560"
     CDS             1421..1864
                     /locus_tag="VFV25_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY1392030.1"
                     /translation="MNVSTSRKLSHRLQRGDLLAEACPSREVLKHLTSRWGVLVLMAL
                     EIRPHRFAELRRAVQGVSERMLAQTLQWLEGDGLVQRIVHDVVPPHVEYRLTPLGREG
                     AKRVRGLADWIEVNLSRVQEFQARRSAKAAEKDSSRSASRVHRMA"
     gene            complement(1876..>2726)
                     /locus_tag="VFV25_01565"
     CDS             complement(1876..>2726)
                     /locus_tag="VFV25_01565"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEY1392031.1"
                     /translation="PNGQGIAALIALGILSRFDMAALPPDSIEAQHLQIEAMKLAFAD
                     VYRYVAEPRSMELTPAQMLDAGYLAARASLIDMKRAQDFGAGNPAKGGTVYLTTADES
                     GMMVSFIQSNYMGFGSGVVVPQFGLSMQNRGHAFSLAAAGPNVVAPGKRPFHTIIPAF
                     LTREVGASVTLPVMSYGVMGANMQPQGHVQTLVRMLDYKQNPQAACDAPRWRYNAGLE
                     LNVEAAMDPAVVQGLLDRGHRIEVIRDSYQDFGAGQFIWRLGDPAREGYVAASDPRRD
                     GQAVGY"
BASE COUNT          460 a          891 c          935 g          440 t
ORIGIN      
        1 cacccgctgc gcctgaatcc tgtgcctgcg ttgcggcaca atccgcgcgc ccaatccggg
       61 tgaaacacag aatgacactg aatgaacaaa gctgaactga tcgatctgat cgcaagctcg
      121 cacgagttct ccaaggccga agcgggccgc gttctcggca cggtgctcga cgccgtcgtc
      181 accaccgtca agaagggcgg ctctgtgacg ctgccgggtt ttggttcgtt caagcaggtc
      241 tcgcgcgccg cgcgctctgg ccgcaacccg gccaccggcg acaagatcaa gatcgctgcc
      301 gccaagctgc cgaagttcac ggccggcgcc gcgttcaagg ccgctgtgga ccccaaggcc
      361 gccgcccgca aggccgagaa ggccgctggc aagaagcccg cgaagaaggc cggcaagaag
      421 gcctgaggcc cagccgcaac cgtcttgatg aaggggcctg cgggcccctt tttctgcttt
      481 tcaaagaccg aaagaaggcg cgggatcagc gcaatgccgc ggccacaacc gcggcaagtg
      541 gcttggtcgg ccgcccgatc aggcgcgcga gctgccggcc gtcgtcgaac agcgcgccgt
      601 tcgacgctcc gacgtcccag ctcgcgatgg cctgcgcgat gcctgcaggc aggccgaagc
      661 cggtcaaggc cgttgcatag tccgcctcgg gcaggttcct gaagggaaaa gccttgccgg
      721 tctggcgcga tacctcggct gccaggtcgc ccagcgtgta ggcctcgtcg ccggccagct
      781 cgtaagtctt gccttcatgt ccgtccgagg tcagcaccgc ggccgctgca gcagcatagt
      841 cggcacgcgc ggccgatgag atacggccct ggcccgcact gccgatgaag gcgccaccgg
      901 ccagcgcacc ggggatcgag cccgtgtagt tctcggtgta ccagccgttg cgaagaatcg
      961 tgaaggccag gccggaagct ttcaacgcgg cctcagtgtc gagatgctcc tgcgccaggc
     1021 tcagcggcga ggtgtccgcg tgaagcaggc tggtgtagac gatgcgcttg acgccggcct
     1081 tcttggccgc ctcgatgaca ttgcggtgct gcgcggcgcg ctgcccgatt tcgctcgagg
     1141 aaatcagcag cagcgtgtcg acgcccgcca gagcggccgg tagcgtttcg gggcgcgcgt
     1201 agtcggcttc gcgcgcgggg atgccgaggt ccgccgcctt ggccggcgaa cgcacgagag
     1261 cgatgagctg ccccggcggc agcgtggcct tgagttgcga gacaacgagg cgcccgagct
     1321 ggccggtggc gccggtaatg gcgatggtca tggtgtgctt ccacttgttg gcgagggagc
     1381 cccactgtac gattggtact tccttttcgt aagtacaaac atgaatgtaa gtacctccag
     1441 aaagctgtcg catcgcctgc agcgtggcga cctgctggcc gaggcctgcc cgtcgcgcga
     1501 ggtgctcaag cacctcacca gccgctgggg cgtgctcgtg ctcatggcgc tggagatccg
     1561 gccgcaccgc ttcgcggagc tgcgccgcgc cgtccaaggg gtcagcgaac gcatgctcgc
     1621 gcagaccttg cagtggctcg agggcgacgg gctggtgcag cgcatcgtcc acgacgtggt
     1681 gccgccgcat gtcgagtacc gcttgacgcc gctggggcgc gaaggagcaa agcgggtgcg
     1741 tggcctggca gactggatcg aggtcaacct ctccagagtg caggagtttc aggctcgccg
     1801 ctcagccaag gccgcggaga aggactcttc gcggagtgcc tcgcgggttc atcgcatggc
     1861 ctaggtcacc cgtcgtcaat agcccactgc ctgcccatcg cgccgcgggt cgctcgcagc
     1921 cacatagccc tcgcgggccg ggtcgccaag tcgccagatg aactggccgg caccgaagtc
     1981 ctggtagctg tcgcggatga cctcgatacg atggccgcgg tcgagcaggc cttgcaccac
     2041 tgccgggtcc atggcggctt cgacattgag ctccagccca gcgttgtagc gccagcgcgg
     2101 cgcgtcgcag gcagcctgcg ggttctgctt gtaatcaagc atgcgcacca gggtctgcac
     2161 atggccctgc ggctgcatgt tggcgcccat cacgccgtag gacatgaccg ggagggtgac
     2221 ggaggcgccc acctcgcggg tgaggaaggc cggaatgatg gtgtggaagg gccgcttgcc
     2281 cggcgccacg acattggggc cggccgcggc cagcgagaag gcatgcccgc ggttctgcat
     2341 cgaaaggccg aactgtggca ccacgacgcc ggagccaaag cccatgtagt tgctctggat
     2401 gaagctcacc atcatgccgc tctcgtcggc cgtggtcaga tacacggtgc cgcccttggc
     2461 ggggttgccg gcgccgaagt cctgagcgcg cttcatgtcg atcaagctcg cgcgcgcggc
     2521 gagatagccg gcatcgagca tctgcgccgg cgtgagctcc atgctgcgcg gctcggcgac
     2581 gtagcggtac acgtccgcaa aggccagctt catcgcctcg atctgcagat gctgggcctc
     2641 gatgctgtcg ggcggcaagg cagccatgtc gaaacgggac aggatgccca aagcgatcag
     2701 cgcggcgatg ccctggccgt tgggcg
//
LOCUS       DASUCY010000075         2122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_965389, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000075 DASUCY010000000
VERSION     DASUCY010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965389"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VFV25_01570"
     CDS             <1..275
                     /locus_tag="VFV25_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392032.1"
                     /translation="ASLGYRTETWGANAAVSSVGARPDVDSNFSQVQLPSYAMVDLSA
                     HYAFARQWRFETKLLNAFDKRYEPVKDYGSLGRSFWVGLRYDTLGF"
     gene            283..1266
                     /locus_tag="VFV25_01575"
     CDS             283..1266
                     /locus_tag="VFV25_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEY1392033.1"
                     /translation="MRPPPRLALWLPMAVATAFAALALGLAAGSSGWGWADAQIVWQI
                     RAPRVLAGFGAGAALALAGALMQLLTRNALADPYVLGVSGGASVGALLALLLAPLAWS
                     FWAPALGAALGALLATALLFALAWRALGRANALASAGEGSVSLLLIGVMLGAAASALI
                     SLLLTLAPDLQLRGMVFWLLGDLNGATEWRPVWLALALGLALVWPRAHELDLLARGEA
                     WAASLGVPVALRRRLALLAAALMTGAAVATTGAVGFVGLVMPHLMRLAGLRAAAVLLP
                     ASALAGGIFLCLADTAARTIVAPLQLPVGVLSAAVGVPVFLALLLHRGSGR"
     gene            1263..2078
                     /locus_tag="VFV25_01580"
     CDS             1263..2078
                     /locus_tag="VFV25_01580"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY1392034.1"
                     /translation="MKPHAPSVQAQGLSLRAGGQGRLLCSGLDLALQAGECWVLLGPN
                     GAGKSTLIATLAGLLLPSEGDVNLAGRSLHAWPLQALARQRAWCPQFWSDPFPASVNE
                     TLRQARLGPARWWPWSDDEDAAFEAELARVLQRLDLAHLAGADVRTLSGGERQRVAVA
                     TCLLQGVPWLLLDEPASHLDLAHQQLLVALLREHADKGGIVMLSLHDLNLAASLASHV
                     VLMDGRGGVLAGPRAAVMTPERLSAAFDVKIDQVEVCGQQRYWIGPATGARVA"
BASE COUNT          229 a          703 c          804 g          386 t
ORIGIN      
        1 ccgcgagcct gggctaccgc accgagacct ggggtgcgaa tgccgccgtg agctcggtcg
       61 gcgcgcggcc tgatgtggac tccaacttca gccaggtgca gctgccttcc tatgcaatgg
      121 tcgatttgag cgcccactac gccttcgcgc gtcagtggcg tttcgagacc aagttgctca
      181 atgcgttcga caagcgctac gagccggtca aagactacgg ctcgctgggc cgcagcttct
      241 gggtcggcct gcgctacgac acgctgggct tctgagtctg caatgcgtcc gccgccgcgc
      301 ctcgccctgt ggctgccgat ggccgtcgcg acggccttcg cggcgctggc gctgggcctc
      361 gcggccggct catcgggctg gggctgggcg gatgcgcaga tcgtctggca gatccgcgcg
      421 ccgcgcgtgc tggccggctt cggtgctggc gcggcgctcg cgctagccgg cgcgctgatg
      481 cagctgctga cgcgcaatgc cctggccgac ccttacgtgc tgggcgtgtc gggcggcgcg
      541 tcggtaggcg cactgctggc cttgttgctc gcgccgttgg cctggagttt ctgggcgccg
      601 gcgctcggtg ctgcgctggg cgctttattg gccacggcgc tgctgtttgc gctcgcctgg
      661 cgcgcactgg gccgtgccaa tgcgctggcc tcggcaggcg agggcagcgt gagcctgctg
      721 ctgatcggcg tgatgctggg cgccgccgct tcggcactga tttcgctgct gctcacgctg
      781 gcgcccgact tgcagctgcg tggcatggtg ttctggctgc tcggcgacct gaacggtgca
      841 accgaatggc ggccggtctg gctcgcgctg gcgctgggcc tggcgctggt ctggccgcgt
      901 gcgcacgagc tggacctgct cgcgcgcggc gaggcctggg ccgcgagcct gggcgtgccg
      961 gtggcgctgc ggcggcgctt ggcgctgttg gcggcggcct tgatgaccgg agcggccgtc
     1021 gcgaccacgg gcgccgtcgg ctttgtcggc ctcgtgatgc cgcatctgat gcggctcgcc
     1081 gggctgcgtg ccgcggctgt cctgctgcct gcgagcgcgt tggccggcgg catcttcctg
     1141 tgcctggccg acacggcggc gcgcaccatc gtcgcgccgc tgcagctgcc ggtgggcgtc
     1201 ttgtcggccg cggtcggcgt gccggtcttc ctggcattgc tgctgcatcg cgggagtggg
     1261 cgatgaagcc gcatgcgccg tcggtgcagg cgcagggctt gagcctgcgc gcgggcgggc
     1321 aggggcggct cttgtgcagc ggcctggacc ttgcgctgca agccggcgaa tgctgggtct
     1381 tgctgggacc caatggcgcg ggcaagtcca cgctgattgc gacgctggcc gggctgctgc
     1441 tgccgtccga gggcgatgtg aatttggccg gccggtccct gcatgcctgg ccgttgcagg
     1501 cgctcgcgcg gcagcgcgcc tggtgcccgc agttctggag cgatcccttt ccggcgagcg
     1561 tgaacgagac gctgcgccag gcgcgcttgg ggccggctcg ctggtggccc tggagcgatg
     1621 acgaggatgc cgccttcgaa gccgaactcg cgcgggtgct gcaaaggctg gacctcgcgc
     1681 atctagcggg cgccgatgtg cgcaccctgt ccggcggcga gcgccagcgc gtcgccgtgg
     1741 cgacctgcct gctgcaaggc gtaccctggt tgctgctcga cgagcccgct tcgcacctgg
     1801 atctcgcaca ccagcagctg ctcgtagcct tgctgcgcga gcatgccgac aaaggcggca
     1861 tcgtcatgct gagcctgcac gacctcaacc tggcggcgag cctcgccagc catgtcgtgc
     1921 tgatggacgg ccgcggcggt gtgctggccg ggccgcgagc tgcggtgatg acacccgagc
     1981 gcctgagcgc tgccttcgac gtgaagatcg accaagtcga ggtctgcggc cagcaacgct
     2041 attggatcgg gccggcgact ggggcgaggg tggcatgaac actactgggc gttggatggt
     2101 tgcggcactg ctggccctgt ac
//
LOCUS       DASUCY010000076         2885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1479500, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000076 DASUCY010000000
VERSION     DASUCY010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1479500"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(262..2532)
                     /locus_tag="VFV25_01585"
     CDS             complement(262..2532)
                     /locus_tag="VFV25_01585"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY1392035.1"
                     /translation="MSDAIRVLLVEDNEMDAELLSRQLHVGQLGADIERVDSAETLQA
                     ALAAGGHDIIISDYTLPGFSGMQALEMAQQQRPEVPFVFVSGTIGEERAIEALKHGAV
                     DYLLKDRPSRLVPCLRRALDQAQEQRTRHSLERKLARLSHIHALMAGLNAAILRGTDG
                     ASLFDEACRLAVEVGGYRFAWIGVTAPRGSKVKPVAFVGHEDGYLAEIGHLLGDAPGH
                     DPVIGLSTVVATDDGGLAGQTLCAGLVFCCNDIETDPMVAFRQQALARGYRSLAALPL
                     SAGAGQLAGVMMLYAADKDYFGDDDIRLLGDLAADLSFALELLDKRARLTQASRDDPL
                     TGLANRSQFFDRLDALTEASKSSQTGLAVVVLDLQRFREINSMLGRAGGDRVLQTFAG
                     RLKRAFDGAATVARMGADRFAVAIPTPRKWSPSTLAAEAKVKALAAPARILGSEVSIA
                     FKMGVATCPADSDHPELLFQQAEAALQRAKDSIELCVFYSPSLNEGVTRRLRMEAKLR
                     RAMRERGFVLHYQPKVRLVDRSVVGLEALIRWNDEPQGLISPAHFVPLLEETGLIAEV
                     GLWVVRQAIADARTWEAQGVGVPRIAVNVSALQLRRPYFAEELLAVLQEAGPGWHGID
                     LEITESQLLEDHGQSTKKLARLRARGLRVYMDDFGTGYSSLSQLSSLPLDALKIDRAF
                     VHDMGKKPEHAAVVRAVIQLADALGLGVVAEGVETEAQAQQLEQLGCGEAQGYHFSRP
                     LAADAIARAGQGFGGP"
     gene            2803..>2885
                     /locus_tag="VFV25_01590"
     CDS             2803..>2885
                     /locus_tag="VFV25_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY1392036.1"
                     /translation="MASHGRRGMRALLLGSETHKVLTHTQIP"
BASE COUNT          490 a         1024 c          914 g          457 t
ORIGIN      
        1 aagtcctgaa tccagcgctg gcgcacgtca ccggcggcag cggcaagatc tgcatggagc
       61 cgcacctggt gccgggcggc gcatggatcg cgccctgcct cgatcctcag ggcggcgcct
      121 tcgccctggt cgccgacaag cgctgaggtc ggcaacacga agggagcgag cccatgatcg
      181 acatgccaga gatcgcccgg gccccgtcgc gccccatcgc cagcatcccc ctgtgcatca
      241 ggggcggcga gacgagcaag ctcatgggcc cccaaacccc tggccggccc tcgcgatcgc
      301 atccgcagcc agcggccggc tgaagtgata cccctgcgct tcgccgcagc cgagttgctc
      361 gagctgctgc gcttgcgcct ccgtctccac cccttcggcg accacgccca gtccgagcgc
      421 atcggccagc tggatcaccg cgcgcaccac tgcggcgtgc tccggcttct tgcccatgtc
      481 gtgaacgaag gcccggtcga tcttcagggc atccagcggc agcgacgaca actggctgag
      541 actggaatag ccggtgccga agtcgtccat gtagacgcgc aggccccggg cccgtaggcg
      601 tgcgagcttt ttggtgctct gcccgtgatc ctccagcaac tggctctcgg tgatctcgag
      661 gtcgatgcca tgccacccag ggccggcttc ctgcagcacg gccagcagct cctccgcaaa
      721 ataaggccgc ctgagctgca gcgccgagac attgacggca atgcgcggca cgcccacccc
      781 ctgggcctcc caggtccggg cgtcggcgat ggcctgacgg acaacccaca ggccgacctc
      841 ggcgatcagg ccggtctcct ccagcaacgg cacgaagtga gccggcgaga tcagtccctg
      901 cggttcgtcg ttccagcgga tcaacgcctc caggccgacg acgctgcggt cgaccaagcg
      961 aaccttgggc tggtaatgca gcacgaagcc acgttcccgc atggcacgtc tcagcttggc
     1021 ttccatgcgc aggcgccgcg ttacgccttc gttgaggctc ggcgagtaga agacgcacag
     1081 ctcgatgctg tccttggctc gctgcagtgc agcctcggcc tgctggaaca gcaattctgg
     1141 gtggtcgctg tcggccggac aggtcgccac gcccatcttg aaggcgatgc tcacctcgct
     1201 gccgagaatg cgagctggtg ccgccaatgc cttaaccttg gcctcggcgg ccagcgtcga
     1261 tggtgaccat ttgcgcggcg tcgggatcgc taccgcgaag cgatcggcgc ccatgcgcgc
     1321 caccgtggcg gcgccgtcga aggctcgctt cagccggcca gcgaaggttt gcagcacgcg
     1381 gtcgccgccg gcacgcccca gcatgctgtt gatctcgcgg aagcgctgca ggtccagcac
     1441 cacgacggcc aggccagtct ggctcgactt ggacgcctcc gtcagcgcgt caagccggtc
     1501 gaagaactgg ctgcggttgg ccaggccggt gagcggatcg tcccgcgacg cctgcgtcag
     1561 gcgggctcgc ttgtccagca gctccagcgc aaacgacagg tcggccgcga gatcgcccag
     1621 caggcggatg tcgtcgtcgc cgaagtagtc cttgtcggcg gcgtacagca tcatcacgcc
     1681 ggccaactgg ccggcgcctg ccgacagcgg cagcgccgca agcgaacggt agccgcgagc
     1741 caaggcctgc tggcggaagg cgaccatcgg atcggtctcg atgtcgttgc aacagaacac
     1801 cagaccggcg cacagggtct gcccggccaa gcccccatcg tcggtcgcga cgactgtcga
     1861 caggccgatg actggatcgt gcccgggcgc gtcgcccagc agatgaccga tctctgccag
     1921 gtagccgtct tcgtgaccga cgaacgccac cggcttcacc ttggagcccc tgggggcggt
     1981 cacgccgatc caggcgaagc ggtagccgcc gacctcgaca gcaagccggc aagcctcgtc
     2041 gaacaggctg gcgccgtcgg tgccccgcaa tatggccgcg ttgaggccgg ccatcaaggc
     2101 gtggatgtgg ctgagccgtg cgagcttgcg ctccagcgag tgacgcgtgc gctgctcctg
     2161 cgcctggtcc agcgcacggc gcagacaggg caccagcctg gaaggccggt ccttcagaag
     2221 gtagtcgacc gcaccgtgct tgagcgcctc gatggcgcgc tcttcgccga tggtgccgga
     2281 gacgaatacg aagggcacct ccggcctttg ctgctgcgcc atctccagcg cctgcatgcc
     2341 cgagaaaccc ggcagcgtgt agtccgagat gatgatgtcg tgcccgccag cggccaaggc
     2401 ggcttgaagc gtctcggctg agtccacgcg ttcgatgtcg gcgcccagct ggccgacatg
     2461 cagctgacgg ctgagcaatt ccgcgtccat ctcattgtcc tcgaccaaca agactctgat
     2521 cgcgtcggac atgcggcctc cctgcatcga agcagagtca tccctcaggc aatctatcgg
     2581 acaacccacg ggctgtcacc tgtaagacct gacatggtca caccttcccc tcgctctcag
     2641 ctgccgcccg aactcggcag ttggcggacc agtacctgag caagctgtcg gcctgcgcga
     2701 cctcctccga tgtcttctgc gacgcgctca ccgttgaaca cgatcacccc tacgaagcga
     2761 tcatcgccac ctgcgcagac gcgtcactgc gacctcatcg taatggcctc gcacggccgg
     2821 cggggcatgc gtgcgctgct gctgggcagc gaaacccaca aggtgttgac gcacacccag
     2881 atccc
//
LOCUS       DASUCY010000077         2051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_114050, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000077 DASUCY010000000
VERSION     DASUCY010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114050"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VFV25_01595"
     CDS             complement(<1..518)
                     /locus_tag="VFV25_01595"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HEY1392037.1"
                     /translation="MLTQQEARPPLAGTSPRPLVICVDDAAQDEAVTEAVLQLAAAGR
                     ISATSAMVQAPGWPAQARSLAAESRIDLGLHLNFTQPFPEASAWPLSQVMRRAWLRGF
                     ARDWLEAQIDAQIEAFANHAGRLPDHIDGHQHVHQFPQIREALLARMAHWWHGAARRP
                     WLRCTLPAPGAA"
     gene            633..>2051
                     /locus_tag="VFV25_01600"
     CDS             633..>2051
                     /locus_tag="VFV25_01600"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY1392038.1"
                     /translation="MQRLAWLPFALLLWWTWSIALRPISVPDEGRYAGVARDMMAHGD
                     WLVPRLNGLPFFHKPPLFYWIDASAMSLFGVGPATARIGSAIAALIACWAVGWHARRV
                     GGQAAMRATLLVLCTMPLYFFGGQYANLDMLVGALITACVVCWAGALDPGGAGDDAAA
                     RRRYACIGWAAAALAILAKGLIGLVLPLLVLGPWALWHWRGRWLAGLRFLLDPLGLVI
                     FAVIVAPWFVAMAQRFPDFLDYFFIEQHFRRYLGSSFNNRQPLLFYPAVLAVLLLPWS
                     LLLVAALRRRGQATVPASPFRSLWIWWAVAISLFFSAPASKLVGYILPAVPPLALLAG
                     SWLAMPGRERWLRGGALLAALVCVGGVFGARQWDTKSLRELPPVMRAAATADPAWRDV
                     PMVFIEAPHFEVPLLLDAKVLPWVVKNWDDPKVQQVDSWPKELFDAARFDPEQGRRVL
                     LKPQDLLARLCTTQRAWILAPLQ"
BASE COUNT          229 a          729 c          721 g          372 t
ORIGIN      
        1 ctcgccgcgc ccggcgccgg tagtgtgcag cgcagccaag gccggcgcgc cgcgccgtgc
       61 caccaatggg ccatgcgtgc cagcagggcc tcgcggattt gcgggaactg gtgcacgtgt
      121 tgatggccat cgatgtggtc gggcagccgg ccggcgtggt tcgcgaaggc ctcgatctgc
      181 gcgtcgatct gcgcctcgag ccagtcgcgt gcgaagccgc gcagccaggc gcggcgcatc
      241 acctgcgata gcggccaggc gctggcctct ggaaagggct gcgtgaaatt cagatgcagg
      301 cccagatcga tgcggctttc ggcggcgagc gagcgggcct gggccggcca gccgggcgct
      361 tgcaccatcg cgctggtggc gctgatgcgg ccggccgcag ccagctgcag cacggcctcg
      421 gtgaccgctt cgtcctgggc cgcgtcgtcg acacagatga cgaggggccg gggcgacgtg
      481 ccggccagcg gcgggcgcgc ttcttgttgt gtcaacattg ccgtctatta tccaatcgcg
      541 tccctcgctg ccggggcacc gccagcgttt gctccatgtc ttcccagccc gcccaaacct
      601 ccactaccga ccaactcggc aacagagctg ccgtgcagcg ccttgcctgg ctcccgttcg
      661 cgctgctgct gtggtggacc tggagcattg cgctgcggcc gatctccgtt cccgacgagg
      721 gtcgctacgc cggcgtggca cgcgacatga tggcccatgg cgactggctg gtgccgcgtc
      781 tgaacggcct gcccttcttc cacaagcccc cgctcttcta ctggatcgac gcgagcgcga
      841 tgtcgctgtt cggcgtcggg cccgcaacgg cgcgcatcgg ctcggccatc gctgcgctga
      901 tcgcctgctg ggccgtgggt tggcatgcac gccgcgtcgg cgggcaggcc gcgatgcggg
      961 ccacgctgct ggtgctgtgc acgatgccgc tgtatttctt cggcgggcag tatgcgaatc
     1021 tcgacatgtt ggtcggcgcc ctgatcacgg cttgcgtcgt ctgctgggcc ggcgcgctgg
     1081 accccggcgg cgccggcgat gacgctgccg cgcgccgccg ctacgcgtgc atcggctggg
     1141 cggccgcggc gcttgcgatc ctcgccaagg gcctgatcgg gctggtgctc ccgctcctgg
     1201 tgctgggccc ctgggctctg tggcactggc gcgggcgctg gctggccggc ttgcgcttcc
     1261 tgctggaccc gctgggcctg gtgatcttcg ccgtcatcgt agcgccctgg ttcgtcgcga
     1321 tggcgcagcg cttccccgac ttcctcgact acttcttcat cgagcagcat ttccgccgtt
     1381 atctgggcag cagcttcaac aatcgccagc ccctgctgtt ctacccggcc gtgctggcgg
     1441 tgctgctcct gccctggagc ctgctgctgg tcgccgcctt gcgccggcgc ggccaggcca
     1501 cggtgccggc ctcccctttc cgcagcctgt ggatctggtg ggcggtcgcg atcagcctct
     1561 tcttctccgc gccggcctcc aagctggtcg gctacatcct gccggccgtg ccaccgctgg
     1621 ccctgctcgc cggctcctgg ctcgccatgc cggggcgcga gcgctggctg cgcggcggcg
     1681 cgctgctggc cgcgctggtt tgcgtcggcg gcgtgttcgg tgcgcggcaa tgggatacca
     1741 agtcgctgcg cgagctgccg cccgtgatgc gggctgccgc gaccgccgac ccggcctggc
     1801 gcgacgtgcc catggtcttc atcgaggcgc cgcacttcga ggtgccgctg ctgctcgacg
     1861 ccaaggtcct gccctgggtg gtgaagaact gggacgaccc caaggtgcag caggtcgaca
     1921 gctggcccaa ggagctgttc gacgcagccc gcttcgatcc ggaacaaggc cggcgcgtgc
     1981 tgctgaagcc gcaggacctg ctggcccggc tttgcactac gcagcgtgcg tggatcctgg
     2041 cgccgctgca g
//
LOCUS       DASUCY010000078         2404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_547866, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000078 DASUCY010000000
VERSION     DASUCY010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547866"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="VFV25_01605"
     CDS             complement(<1..307)
                     /locus_tag="VFV25_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili twitching motility protein PilT"
                     /protein_id="HEY1392039.1"
                     /translation="MSTNMERVLRLMADKQASDVYLSVNTPILIKIHGQILQLSDQPL
                     TPQQPRQLLSELLTPTQMEELDDTGELNIGIGLQGVASFRLSAFKQRGSVAAVFRCIP
                     "
     gene            complement(357..1178)
                     /gene="dapD"
                     /locus_tag="VFV25_01610"
     CDS             complement(357..1178)
                     /gene="dapD"
                     /locus_tag="VFV25_01610"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEY1392040.1"
                     /translation="MSQDLQSIIDLAWEARAQITPTSAPEVRATVEKVITELNAGRLR
                     VAEKISGEWVTHQWLKKAVLLSFRLHDNRLMRAGELGFYDKVPTKFSDMSEDELRVSG
                     VRVVPPAVARRGSYLAKNVVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEENSVISMGVYIGQSTKIYDRA
                     TGEVSYGRIPAGSVVVSGSLPSADGKYSLYCAVIVKRVDAQTRAKTSINDLLRGS"
     gene            complement(1189..2391)
                     /gene="dapC"
                     /locus_tag="VFV25_01615"
     CDS             complement(1189..2391)
                     /gene="dapC"
                     /locus_tag="VFV25_01615"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEY1392041.1"
                     /translation="MNPLLSRLQPYPFERLRALFANVVPPAQHRPISLGMGEPKHPTP
                     AFIKEALAAALDGLANYPLTIGDPALREACAAWLEQRYGVAVDPATQLLPVNGSREAL
                     FALAQTVIDPTRPGATVVCPNPFYQIYEGAALLAGAQTAFANSDPRLNFAADWSAIPE
                     ETWARTQLLYVCSPGNPTGAVMPLAEWQRLFELSDRHGFVIASDECYSEIYFRDEAPL
                     GGLEAAAKLGREDFRRLIAFTSLSKRSNVPGLRSGFVAGDAAVIKAFALYRTYHGSAM
                     GPAVQRASIAAWGDEEHVRANRELYRRKFARVTPMLAEVMEVALPDAGFYLWAKVPER
                     LWGGSDTAFARELLAQYNVTVLPGSYLAREAQGVNPGLGRIRMALVAGVDECVEAAER
                     IVSFIRSN"
BASE COUNT          383 a          788 c          815 g          418 t
ORIGIN      
        1 tggggatgca gcggaacacc gccgccaccg agccgcgctg cttgaaggcc gagagccgga
       61 agctcgctac cccctgcagc ccgatgccga tgttgagctc gccggtgtcg tccagctctt
      121 ccatttgcgt cggcgtcagc aattcgctga gcagctggcg cggctgctgc ggcgtcagcg
      181 gctggtccga cagctgcagg atctgcccgt ggatcttgat caggatgggc gtgttcaccg
      241 aaaggtagac gtccgaggcc tgcttgtcgg ccatcagcct cagcacccgc tccatgttcg
      301 tgctcatcct gctccctctt gtgctcgatg acgctctcgc ccgcgctgag caggtctcag
      361 ctgccgcgca gcaggtcgtt gatgctggtc ttggcccgcg tctgcgcatc gacgcgcttg
      421 acgatcacgg cgcaatacag gctgtacttg ccgtcggcgc tgggcagcga accgctcacg
      481 accaccgagc cggccgggat gcggccgtag ctcacttcgc cggttgcgcg gtcgtagatc
      541 ttggtgctct ggccgatgta gacgcccatc gaaatcaccg agttctcctc gacgatcacg
      601 ccctcgacga cctcggagcg cgcgccgatg aagcagttgt cctcgatgat ggtggggttg
      661 gcctgcagcg gctccagtac gccgccgatg cccacgccgc ccgacaggtg gacgttcttg
      721 ccgatctggg cgcaggagcc cacggtagcc caggtgtcga ccatggtgcc ttcgtccaca
      781 taggcgccga tgttgacgta ggagggcatc agcacgacgt tctttgcgag atagctgccg
      841 cggcgcgcca cggccggcgg caccacgcgc acgcccgaga cgcgcagctc gtcttcgctc
      901 atgtccgaga acttggtcgg gaccttgtcg tagaagccca gttcgccggc gcgcatcagg
      961 cggttgtcat gcaggcggaa gctcagcagc accgccttct tgagccattg gtgagtgacc
     1021 cattcgcctg agatcttttc ggccacgcgc agccggccgg cgttgagctc ggtgatgact
     1081 ttctcgacgg tggcacggac ttcgggcgcc gacgtcggcg tgatctgcgc gcgggcctcc
     1141 caggcgaggt cgatgatgct ttggaggtct tgagacatgt tcgttcgctc agttggatcg
     1201 tatgaaggag acgatgcgct cggcggcctc gacgcattcg tcgacacccg ccaccagcgc
     1261 catgcggatg cgccccaggc cgggattcac gccctgagcc tcgcgcgcca gatagctgcc
     1321 gggaagtacg gtcacattgt attgagccag cagttcgcgg gcgaaggcag tgtccgaacc
     1381 gccccacagg cgctccggga ccttggccca gaggtagaag ccggcgtcgg gcagggccac
     1441 ttccatcacc tcggccagca tgggcgtgac gcgggcgaac ttccggcggt agagctcgcg
     1501 attggcgcgc acatgctctt cgtcgcccca ggctgcgatg ctggcgcgct gcaccgccgg
     1561 gcccatggcg ctgccgtggt aggtgcggta gagcgcgaag gccttgatga cggccgcgtc
     1621 gccggccacg aagcccgagc gcagtcccgg cacattggaa cgcttggaca ggctggtgaa
     1681 ggcgatcagc ctgcggaagt cctcgcggcc cagtttggcc gcggcctcca ggccgcccaa
     1741 cggcgcctcg tcgcggaaat agatctccga atagcactcg tccgaggcga tcacgaagcc
     1801 gtggcggtcg ctcagctcga acaaacgctg ccattcggcc agtggcatca cggcgccggt
     1861 tgggttgcct ggcgaacaga catagagcag ctgcgtgcgc gcccaggttt cctcgggaat
     1921 ggccgaccag tcggccgcga aattcaggcg cgggtcgcta ttcgcgaagg ccgtctgcgc
     1981 accggccagc agggccgcgc cctcgtagat ctggtagaaa ggattagggc agacgaccgt
     2041 ggcgccaggc cgcgtcgggt cgatcacggt ctgcgccagc gcgaacaggg cttcgcgcga
     2101 tccgttgacg ggcagcaact gggtcgctgg gtccaccgcg accccatagc gctgctccag
     2161 ccatgccgcg caggcctcgc gcagggccgg atcgcctatg gtcagcgggt agttcgccag
     2221 gccgtccagc gccgccgcca atgcctcctt gatgaaagcc ggtgtcggat gcttgggctc
     2281 gcccatgccc aggctgatcg ggcggtgctg ggccggtgga acaacgttgg cgaacaaggc
     2341 gcgcagccgc tcgaacgggt agggctggag gcgcgaaagc agcgggttca tggcgccgat
     2401 tatc
//
LOCUS       DASUCY010000079         4408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_451530, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000079 DASUCY010000000
VERSION     DASUCY010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4408
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451530"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..1082
                     /locus_tag="VFV25_01620"
     CDS             27..1082
                     /locus_tag="VFV25_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006495079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEY1392042.1"
                     /translation="MRTTGASWRWPSGSRRPAPGWRTRWAAATSRMPMDTTSISAPES
                     PQAAVLSAALLRIQHETRYQYAGPVELAHHMAFLRPLEDAAQHVEAFEMRVEPAPSQH
                     STTRDAFGNSRAFFSLTAPHQALSVKACSLLRVVDRYAGLQPAASLPWDAVREQLRYT
                     AGARYAPAAEFAFASPMVPLHAELRAYAAPSFSPGRPLAEAALELMQRVHEDFAYDPR
                     STQVATPVLQAFEQRRGVCQDFAHLTIGCLRALGLAARYVSGYLLTDPPPGQPRLLGA
                     DASHAWVSVYAPGAQPGLDWLDLDPTNACVPALAHARLATGRDYGDVTPLKGVIRGGG
                     QHTLAVHVSTEVVPAPA"
     gene            1161..2594
                     /locus_tag="VFV25_01625"
     CDS             1161..2594
                     /locus_tag="VFV25_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEY1392043.1"
                     /translation="MHPYDEMHAGSKQVREHYSQYDRWLARQPQEMMKTRRDEAEMIF
                     RRVGITFAVYGTKDEEGAGTERLIPFDLIPRIIPSHEWREMERGLRQRVTALNRFLHD
                     VYHDQEIIKAGIVPAEQVFKNVQFRPEMMGVDVPGGIYSHIAGIDIVRAGNPDGSGSY
                     YVLEDNLRVPSGVSYMLENRKMMMRLFPDLFGEHRVAPVAHYPDLLLETLRAVAPAGV
                     NEPVVVVLTPGMYNSAYFEHAFLAQQMGVELVEGQDLFVRDNYLYMRTTQGPKRVDVI
                     YRRVDDDFLDPLVFRPDTSLGCAGLLSVYRAGNVTLANAIGTGVADDKSIYPYVPKMI
                     EFYLGEQPILHNVPTYLCREPEDLKYVLAHLPELVVKEVHGAGGYGMLVGPASTKEEI
                     AAFREQLLLNPGNYIAQPTLSLSTCPTFVESGIAPRHIDLRPFVLSGKTVQMVPGGLT
                     RVALKEGSLVVNSSQGGGTKDTWVLEA"
     gene            2597..3562
                     /locus_tag="VFV25_01630"
     CDS             2597..3562
                     /locus_tag="VFV25_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HEY1392044.1"
                     /translation="MLSRTADHLFWMARYMERAENTARMLDINYQTSLLPQSAATAEQ
                     GWRGLLSISELTLDFHKKYDNVSARAVMDFMVRDEANSSSIVSCLRAARENARAVRGA
                     LTTEVWETQNQTWLEFNRLLQAEVFQKDPSEFFEWVKFRSHLSRGVTAGTMLQDEAYH
                     FLRIGTFLERADNTARLLDVKFHSAQGEYFGARGQGRDQHEVDFYHWSAILRSVSGFE
                     VYRKVYRNVIRPERVAELLMLRPDMPRSLLACMNEVVANLRMVANEQSQETLRRAGRL
                     RADLRFGRIDEILATGLHAYLTQFLDRVGDLGYGISRDFLVPVSA"
     gene            3591..4391
                     /locus_tag="VFV25_01635"
     CDS             3591..4391
                     /locus_tag="VFV25_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEY1392045.1"
                     /translation="MRLHIKHSTHYVYREPVRHAIQTLLLTPQNSPHQRVHRWQLHAP
                     GTLFEARDAYGNTSHTYTLARPIVRGTVRASGEVETLACPEVVDPPGSLSPWLYLRPT
                     PLAEPHRRLAEFTAPHLDAGLNPDSLLALAGAVCDQVTYHPGHTDVQTTALEAFDWGK
                     GVCQDQAHVFIAACRSHGVPARYVSGYFYAADAPELASHAWADVCVDKGEGRWLSIDV
                     THRCLMDERHIRLATGPDYAACPPIKGVRSGGGDETMEVQVEIRPVGS"
BASE COUNT          707 a         1515 c         1487 g          699 t
ORIGIN      
        1 ccatgtcgcc gaggcacctg aaggcgatgc gcacgacggg cgcatcctgg cgctggccga
       61 gcggctcgag gaggccggcg cccggctggc ggacgaggtg ggccgccgct acttcgcgca
      121 tgccgatgga tacgaccagc atctcagcgc ctgagtcgcc acaggctgca gtgttgtcgg
      181 ctgccctgtt gcgcatccag cacgaaacgc gctaccagta cgccggaccg gtggagctgg
      241 cgcaccacat ggccttcctg cgcccgctgg aggacgccgc ccagcacgtc gaggccttcg
      301 agatgcgggt ggagccggcg ccgagccaac acagcaccac acgcgacgct ttcggcaaca
      361 gccgggcctt cttcagtctc accgcgccgc accaggcgct gagcgtcaag gcctgcagcc
      421 tgttgcgcgt cgtggaccgc tatgcgggcc tgcagccggc ggcctccttg ccctgggacg
      481 cggtgcgcga gcagctgcgc tataccgccg gggcgcgcta cgcgccggca gccgagttcg
      541 ccttcgcctc gccgatggtg ccgctgcacg ccgagttgcg cgcctatgcg gcgccttcgt
      601 tcagccccgg ccgaccattg gccgaggccg cgctggagct gatgcagcgc gtgcatgagg
      661 acttcgccta cgacccgcga agcacccagg tcgctacgcc ggtgctgcag gccttcgagc
      721 agcggcgcgg cgtgtgccag gacttcgcac acttgacgat cggctgcctg cgggcgctgg
      781 gcctggctgc acgctatgtg agcggctatc tgctgaccga tccaccgccc ggccagccgc
      841 gcctgctggg cgccgatgcc tcgcatgcct gggtctcggt gtatgcgccc ggcgcgcagc
      901 ccgggctgga ctggctggat ctcgacccga ccaacgcttg cgtgcctgcg ctggcccatg
      961 ccaggctggc caccgggcgc gactacggcg acgtgacgcc gctcaagggc gtgatccgcg
     1021 gcggcgggca gcacaccctt gcggtgcatg tgagcacgga ggtggtgcct gcgccggcgt
     1081 aatgcgcagc tgggcctgcc catgctgggg cgagcttggc acaatccgtg catgccatga
     1141 gaaacatgga aggggacgcg atgcatccgt acgacgaaat gcacgccggc agcaagcagg
     1201 tccgtgagca ctacagccag tacgaccgct ggctggcgcg gcagccgcag gagatgatga
     1261 agacgcgccg cgacgaggcg gagatgatct tccgccgcgt gggcatcacc tttgcggtct
     1321 acggcaccaa ggacgaggag ggggccggca ccgagcgcct gatccccttc gacctgatcc
     1381 cgcgcatcat cccctcgcac gagtggcgcg agatggagcg cggactgagg cagcgcgtca
     1441 ccgcgctcaa ccgcttcctg cacgacgtct atcacgacca ggagatcatc aaggccggca
     1501 tcgtgccggc ggagcaggtc ttcaagaacg tgcagtttcg tcccgagatg atgggcgtgg
     1561 acgtgccggg cggcatctat tcgcacatcg ccggcatcga catcgtgcgc gccggcaacc
     1621 cggacggctc gggcagctac tacgtgctcg aggacaacct gcgcgtgccc agcggcgtga
     1681 gctacatgct cgagaaccgc aagatgatga tgcgcctgtt ccccgacctg ttcggcgaac
     1741 accgcgtggc gccggtggcg cactacccgg acctgctgct cgagaccctg cgtgccgtgg
     1801 cgccggccgg cgtgaacgag cccgtggtgg tggtgctcac gccgggtatg tacaactcgg
     1861 cctacttcga gcatgccttc ctcgcgcagc agatgggcgt ggagctggtt gagggacagg
     1921 acctcttcgt gcgcgacaac tacctctaca tgcgcaccac gcaaggcccc aagcgggtgg
     1981 acgtgatcta ccgtcgcgtg gacgacgact ttctcgaccc gctggtgttc cgccccgaca
     2041 cgtcgctggg ctgcgcaggc ctgctctccg tctaccgcgc cggcaacgtg acgctggcca
     2101 acgccatcgg caccggcgtg gccgacgaca agtcgatcta cccctatgtg ccgaagatga
     2161 tcgagttcta cctcggcgag cagcccatcc tgcacaacgt gcccacctat ctgtgccgcg
     2221 agcccgagga cctgaagtac gtgctggcgc atctgcccga actggtggtg aaggaggtgc
     2281 acggcgccgg cggctacggc atgctggtgg gaccggcctc caccaaggag gagatcgccg
     2341 cgttccgcga gcagctgctg ctcaatcccg gcaactacat cgcgcagccg accctgtcgc
     2401 tgtcgacctg ccccaccttc gtggagagcg gcatcgcgcc gcgccacatc gacctgcggc
     2461 ccttcgtgct ctccggcaag accgtacaga tggtgccggg cgggctgacg cgcgtggcgc
     2521 tgaaggaagg ctcgctggtg gtcaactcgt cgcagggcgg cgggaccaag gacacctggg
     2581 tgctggaggc ttgaccatgc tgtctagaac cgcggatcac ctcttctgga tggctcggta
     2641 catggagcgg gccgagaaca ccgcccgcat gctggacatc aactaccaga cctcgctgct
     2701 gccgcagtcg gccgccacgg ccgagcaggg ctggcgcggg ctgctgagca tttcggagct
     2761 gacgctggac ttccacaaga agtacgacaa cgtcagtgcc cgcgcggtga tggacttcat
     2821 ggtgcgcgac gaggccaatt cgtccagcat cgtcagctgc ctgcgcgccg cgcgcgagaa
     2881 cgcgcgggcg gtgcgaggcg cgctcaccac cgaggtgtgg gagacgcaga accagacctg
     2941 gctggagttc aaccgcctgc tccaggccga ggtctttcag aaggatccca gcgagttctt
     3001 cgaatgggtg aagttccgct cgcatctttc gcgcggcgtc accgcgggca ccatgctgca
     3061 ggacgaggcc tatcacttcc tgcgcatcgg caccttcctg gagcgggccg acaacacggc
     3121 gcggctgctg gacgtgaagt tccattcggc gcagggcgag tacttcgggg cccgcggcca
     3181 ggggcgcgac cagcacgagg tggacttcta ccactggtcg gcgatcctgc gctcggtctc
     3241 gggcttcgag gtctatcgca aggtctatcg caacgtgatc cggcccgagc gcgtggccga
     3301 gctattgatg ttgcgccccg acatgccgcg ctcgctgctg gcctgcatga acgaggtggt
     3361 cgccaatctg cgcatggtcg ccaacgagca gagccaggag acgctgcgcc gcgccggccg
     3421 gctacgcgcg gacctgcgat tcggtcgcat cgacgagatc ctcgccaccg gcctgcatgc
     3481 ctacctcacc cagttcctgg atcgcgttgg cgacctgggc tatggcatca gccgcgactt
     3541 cctggtgccg gtgagtgcct gaggccccgc atccggcgct gcgcaaaccc atgcgcctgc
     3601 acatcaagca ctccacccat tacgtctatc gcgagccggt gcgccacgcg atccagacct
     3661 tgcttctcac gccgcagaac tcgccgcacc agcgcgtgca ccgctggcag ctgcatgcgc
     3721 ccggcacgct gttcgaggcg cgcgacgcct acggcaacac cagccacacc tatacgctgg
     3781 cgcggcccat cgtgcgcggc acggtacgcg ccagcggcga ggtggagacc ttggcctgtc
     3841 cggaggtggt ggacccgccg ggttcgctgt cgccctggct ctatctgcga cccacgccgc
     3901 tggccgagcc gcatcggcgc ctggccgagt tcaccgcgcc gcacctggat gccgggctga
     3961 accccgacag cctgctggcc ctggccggcg ccgtgtgcga tcaggtgacc taccacccgg
     4021 gacacacgga cgtgcagacc acggcgctgg aggccttcga ctggggcaag ggcgtgtgcc
     4081 aggaccaggc ccatgtcttc atcgctgcct gccgcagcca cggcgtgccg gcgcgctatg
     4141 tgagcggcta tttctatgcg gccgatgcgc ccgagctggc gagccatgcc tgggccgatg
     4201 tctgcgtgga taagggcgaa gggcgctggc tcagcatcga cgtcacgcac cgctgcctga
     4261 tggacgaacg tcacatccgc ctcgccacgg ggccggatta cgcggcctgt cccccgatca
     4321 agggagtgcg ctcgggcggc ggcgatgaga cgatggaagt gcaggtggag attcggcctg
     4381 ttggcagtta gtgcagacga atcctcgt
//
LOCUS       DASUCY010000080         3254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_563943, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000080 DASUCY010000000
VERSION     DASUCY010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563943"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VFV25_01640"
     CDS             <1..616
                     /locus_tag="VFV25_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY1392046.1"
                     /translation="LRVGQIAIAIGNPLGFTHTVTSGIVSALGRSLRSTAGRMIDNVI
                     QTDAALNPGNSGGPLLDSRGRVIGVNTAIIPGAQAICFAVAVNSAKWVVTQLFTHGRV
                     RRAYIGLSASTVPISRRVARFLEAEQGSAVRVLVVQPGSPAQIGGLRADDLIVGIDGA
                     PARTVDDLQRLLDESRIGKQCVVRVLRGTQPLYLNVQPVESRRA"
     gene            complement(626..1297)
                     /locus_tag="VFV25_01645"
     CDS             complement(626..1297)
                     /locus_tag="VFV25_01645"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY1392047.1"
                     /translation="MADGTAWLLMELRVRMLRVALIASSPLVRAGLHAALDQAGGFEV
                     LRSAASLDELAGDPPPGLEVVIAQAADDEAAWLAQLPGEGWPPLVLLLEGEARAVGEW
                     LAEGVTVLASDAGADLVAAAARAAAAGLVAGSREMISDALRFARTPRHEERTVPGLER
                     LTARETEVLAQLAQGLGNKAIAQALHISTHTAKFHVAQIISKLDASSRAHAVAKALRA
                     GLIEA"
     gene            complement(1270..2199)
                     /locus_tag="VFV25_01650"
     CDS             complement(1270..2199)
                     /locus_tag="VFV25_01650"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HEY1392048.1"
                     /translation="MTNAAPAVPGAGPLAAYSEALASIVETVGRSALAVPGRHRHGLA
                     SAVVWRPGIVVTAAHVFRHPPAAVSLVGPDGKIIEATLAGIDTPTDVAVFRVAEEGAA
                     PAQLGDPSTLKAGSVVIAVGRSAGGDPTASYGIVNRSSGPWETWLGGQLDRMIRLDGG
                     IYEGLSGAPVADASGAIIGIASSALSRSYGMVVPAATVNRVVDSLLSKGHVARAFLGV
                     SAQPVPLGLGQEGVGLLVTGLVPGGPAQAAGLMVGDILVSVDGKPAASLHELRQGLAD
                     RVGQPVVIRLQRGGVATELALTVGQWPTERRGC"
     gene            2463..>3254
                     /locus_tag="VFV25_01655"
     CDS             2463..>3254
                     /locus_tag="VFV25_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY1392049.1"
                     /translation="MQATDIRHPDYFHKVVDCQWACPAHTPVPEYIRLIAAGRYTDAY
                     LVNWASNVFPGILGRTCDRPCEPACRRGRVEEAQAAKPEPVAICRLKRVAADFKDDIS
                     EQLPRPAAVKNGKRIACVGAGPASLTVARDLAPLGYHVTVFDGDAKPGGMIRSQIPRF
                     RLPEEVIDEETGYVLQLGVEFRAGMRIASMQQLLDENWDAVFVGSGAPRGRDLSLPGR
                     AEAATHIHIGIDWLASVSFGHTTHIGKRVIVLGGGNTAMDCCRTAK"
BASE COUNT          514 a         1210 c         1075 g          455 t
ORIGIN      
        1 gctgcgcgtc gggcagatcg ccatcgcgat cggcaacccg ctgggcttca cgcacacggt
       61 caccagcggc atcgtctcgg ccctgggccg ctccctgcgt tcgacggctg gccgcatgat
      121 cgacaacgtc atccagaccg atgcggcgct gaacccgggc aactcgggcg gtccgctgct
      181 cgacagccgc ggccgcgtga tcggcgtgaa cacggccatc attcccggcg cccaggcgat
      241 ctgcttcgcc gtcgcggtca actcggcaaa atgggtcgtc acccagctgt tcacgcatgg
      301 ccgcgtgcgg cgcgcctaca tcggcctgtc cgcctcgacc gtgccgatct cgcgccgcgt
      361 ggcgcgcttc ctcgaagccg agcagggcag cgcggtgcgc gtccttgtcg tgcagcctgg
      421 ctcgcccgcg caaatcggcg gcctcagggc cgacgacctc atcgtcggca tcgacggcgc
      481 gccggcgcgc acggtggacg acctgcagcg cctgctcgac gagtcgcgca tcggcaagca
      541 atgcgtggtg cgcgtgctgc gcggcaccca gccgctgtac ctgaacgtgc agcccgtcga
      601 atcccggcgc gcctgagtgc ggcgctcagg cctctatcag gccggcccgc agggccttgg
      661 ccacggcgtg ggcgcgtgag ctcgcatcga gcttgctgat gatctgcgcg acatggaact
      721 tggccgtgtg cgtggaaata tgcagcgcct gggcaatggc cttgttcccc aggccctggg
      781 cgagctgggc caggacctcg gtctcgcgcg ccgtcagccg ctccagcccg ggcacagtgc
      841 gctcctcgtg gcgcggcgtg cgggcgaagc gcaaggcgtc cgagatcatc tctctggagc
      901 cggcgaccag gccggccgct gcagcacgcg cggccgccgc gacgagatcg gcgccagcat
      961 cgcttgccag gaccgtaaca ccctcggcca gccactcgcc gacggctcga gcctcgcctt
     1021 ccagcagcag caccaggggc ggccagccct cgcccggcag ctgcgcgagc caggccgcct
     1081 cgtcatccgc cgcctgcgcg atgaccacct ccagcccggg cggcggatcg ccggccagtt
     1141 cgtccagcga cgcggcggag cgcaagacct cgaagccgcc ggcctgatcc agagcggcat
     1201 gcaggccggc ccgcacgagc ggcgagctgg caatcagcgc gaccctaagc atgcgcaccc
     1261 gcagctccat caacagccac gccgttccgt cggccattga ccgaccgtca aggccagctc
     1321 ggtcgccacg ccgccgcgct gcagcctgat caccaccggc tgcccaacgc gatcggccag
     1381 cccctggcgc agctcgtgca ggctggctgc cggcttgccg tccacgctaa ccaggatgtc
     1441 gcccaccatg agcccggcgg cctgcgcggg cccaccgggc accaggcctg tgaccagcag
     1501 gcccacgccc tcctggccga ggcccagcgg caccggctgc gcactcacgc ccagaaaggc
     1561 gcgggccacg tgccccttgc ttaacagcga atcgaccacc cgattcacgg tggccgccgg
     1621 aaccaccatg ccgtagcttc tggacagcgc ggagctggcg atgccgatga tggccccgct
     1681 cgcatccgcc accggagcgc ccgacaggcc ctcatagatg ccgccgtcga gccggatcat
     1741 gcggtcgagc tggccgccca gccaggtttc ccaggggccg gagctgcggt tcacgatgcc
     1801 gtagctggcc gtcggatcac cgccggccga gcgccccacg gcgatgacca cgctgcctgc
     1861 cttgagtgtc gagggatcgc ccagctgcgc gggagctgcc ccctcttcgg cgacgcgaaa
     1921 gaccgcgaca tccgtgggcg tatcgatgcc ggccaatgta gcttcgatga tcttcccatc
     1981 ggggccaacc agcgaaaccg ctgccggcgg gtggcgaaac acatgggcgg ccgtcaccac
     2041 gatgccgggc cgccagacca ctgccgaggc cagcccgtgc cgatggcggc caggcacggc
     2101 cagggcgctg cggcccacgg tttcgacgat ggatgccagg gcctcggaat aggcagccag
     2161 cgggccggca cctggaactg cgggagctgc gttcgtcatg gaatccgtcc tttccgtgta
     2221 tatggaggaa aggtaggccg ggctggccgg caccgcatcc gccgatcggc caggttgcgt
     2281 tcctgaccat attgggaggt ttcggccccg gacaagcccc cacccatgtg agggcggccc
     2341 tcgcacaaac ccgggctgac actgctactg tgcataccta ggattgcggc agccacaagc
     2401 gccatcagtc acacagatgc gcgccgacct caccgggccg gacgcgcgga aaagggggtc
     2461 gattgcaagc caccgacatc cgccacccgg actacttcca caaggtagtc gactgccaat
     2521 gggcctgccc ggctcatacg ccggtgcccg aatacatccg cctcatcgcg gcgggccgct
     2581 ataccgatgc ctacctcgtc aactgggcat cgaacgtctt ccccggcatc ctcggccgca
     2641 cctgcgaccg gccctgcgag cccgcctgcc ggcgcggccg cgtcgaggag gcacaggccg
     2701 ccaagcccga gcccgtagcc atctgccggc tcaagcgtgt ggccgccgac ttcaaggacg
     2761 acatcagcga gcagttgccg cggccggccg ccgtgaagaa tggcaagcgc atcgcctgcg
     2821 tgggcgccgg ccccgcttcg ctcaccgtgg cgcgagacct ggcaccgctg ggctatcacg
     2881 tcacggtgtt cgacggcgat gccaagcccg gcggcatgat ccgctcgcag atcccgcgct
     2941 ttcgcctgcc cgaggaagta atcgacgagg aaacgggcta tgtgctgcag ctgggcgtgg
     3001 agttccgcgc cggcatgcgc atcgcgtcga tgcagcagct gctcgacgag aactgggacg
     3061 cggtgttcgt cggttcaggc gcaccgcgcg ggcgcgatct ctcgctgccc gggcgcgcgg
     3121 aggccgcaac acacatccac atcggcatcg actggctggc ctcggtctcg ttcggccaca
     3181 ccacgcacat aggcaagcgc gtgatcgtgc tgggcggcgg caacaccgcg atggactgct
     3241 gccgcaccgc gaag
//
LOCUS       DASUCY010000081         2164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_821022, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000081 DASUCY010000000
VERSION     DASUCY010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_821022"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /gene="coaE"
                     /locus_tag="VFV25_01660"
     CDS             complement(<1..308)
                     /gene="coaE"
                     /locus_tag="VFV25_01660"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543204.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEY1392050.1"
                     /translation="MQTRIGLTGGIGSGKSVVATMLADLGACLVDTDAIARQITAPGG
                     AAIPALLAQFGAVIIDAHGGLDRERMRTLAFTRPETRRLLESLLHPLIGQEAEHQAAS
                     A"
     gene            complement(327..1184)
                     /locus_tag="VFV25_01665"
     CDS             complement(327..1184)
                     /locus_tag="VFV25_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEY1392051.1"
                     /translation="MGAPELLGALLSWPALGLIGLCVGSFLNVVIHRLPLMLERQWRA
                     ESAELLGVPAKNEGEPLSLSRPRSRCPSCGHQIRWYENIPLVSWLVLRARCSACKAPI
                     SVRYPLFELLTGLLFAAVGWHYGAQPQALLWCGFAATLVALAAIDWDTTLLPDDLTLP
                     LLWAGLIAAALGWTLPLGTAIWGAVAGYLSLWAVYWLFKLTTGKEGMGRGDFKLLAAL
                     GAWLGWQMLVPILLAASVIGAVVGIAMKLSAQLREGRYVPFGPFLAGAGLVVMLAGAP
                     RVVDWLGWA"
     gene            complement(1187..>2164)
                     /locus_tag="VFV25_01670"
     CDS             complement(1187..>2164)
                     /locus_tag="VFV25_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY1392052.1"
                     /translation="AGVPLLQAFDIVARGNPNPRMTKLITDIRADVETGTSLSAAFRK
                     FPMYFDALYCNLIEAGEAAGILETLLDRLATYQEKTLAIKSKIKSALIYPVAVLVVAF
                     VVVSVIMIFVIPAFKEVFTSFGADLPAPTLAVMAMSEIFVKYWWAIFGSIGGGLCFFF
                     QSWKRSEKMQKTMDRLMLKMPIFGDLVNKATVARWTRTLSTMFAAGVPLVEALDSVGG
                     AAGNWVYLEATQKIQKDVSTGAGLTTSMNATGVFPNMVLQMCAIGEESGSIDAMLGKA
                     ADFYEEEVDEMVKGLSTLMEPIIIVILGVIIGGIVVSMYLPIFKLGQVV"
BASE COUNT          400 a          726 c          717 g          321 t
ORIGIN      
        1 gcagaggcgg cctggtgctc ggcttcctgg ccgatcagtg gatgcaatag gctctccagt
       61 aggcggcgcg tctcgggccg ggtgaaggcg agggtccgca tgcgctcgcg gtccagtccg
      121 ccgtgcgcgt cgatgatgac ggccccgaac tgcgcaagta gggcgggaat cgcagccccg
      181 cccggcgccg tgatctggcg ggcgatggca tcggtgtcga ccaggcaggc gccaaggtcg
      241 gccaacatgg tggcgaccac gctcttgccg ctgcctatgc cgccggtgag gcctatgcgt
      301 gtctgcatgg cctcaatgat cgaccatcag gcccagccca gccagtccac cacgcgcggc
      361 gcgccggcca gcatcacgac caagcctgcg ccggcgagga aggggccgaa gggcacatag
      421 cgaccttcac gcagctgggc gctgagcttc atcgcgatgc ccacgacggc accgatcacc
      481 gaggctgcca gcaggatggg caccagcatc tgccagccca gccaggcgcc gagtgcggcg
      541 agcagcttga aatcgccgcg ccccatgccc tctttgccgg tggtcagctt gaacagccag
      601 tagaccgccc acagcgagag gtagccggct acggcgcccc agatcgcggt gccgagcggc
      661 aaggtccaac ctagcgccgc ggcgatgagg ccggcccaga gcagcggcag agtgagatcg
      721 tcgggcagca gcgtcgtgtc ccagtcgatg gcggcaagag ccaccagcgt ggccgcaaag
      781 ccgcaccaca gcagcgcttg cggctgggcc ccatagtgcc agccgacggc ggcgaacagc
      841 agccccgtca gcaactcgaa cagcgggtag cgtaccgaga tgggcgcctt gcaggccgag
      901 cagcgggcgc gcagcaccag ccagctcacg agcgggatgt tttcgtacca gcgaatctgg
      961 tggccgcagg acgggcagcg cgagcgcggc cgcgacaggc tcaagggctc gccctcgttc
     1021 ttggccggca cgcccagcag ctcggcgctc tcggcccgcc actggcgttc cagcatcagc
     1081 ggcagccggt gaatcacgac gttgaggaag ctgccgacgc acaggccgat caggcccagc
     1141 gccggccagg agagcagggc tcccagtagt tcgggcgctc ccacgctcag accacttggc
     1201 cgagcttgaa gatgggcaga tacatcgaca cgacgatgcc gccgatgatg acgcccagga
     1261 tgacgatgat gatgggctcc atcagcgtgg acaggccctt caccatctcg tcgacctctt
     1321 cctcgtagaa gtcggcggcc ttgcccagca tggcgtcgat cgagcctgat tcctcgccga
     1381 tggcgcacat ctgcagcacc atgttcggga acacgccggt ggcgttcatc gaggtcgtga
     1441 ggccggcacc ggtggagacg tccttctgga tcttctgggt cgcttccagg tagacccagt
     1501 tgcccgcggc gccgcccacc gagtcgagcg cctcgaccag cggcacgccg gccgcgaaca
     1561 tggtcgacag ggtgcgcgtc cagcgcgcga ccgtggcctt gttcaccagg tcgccgaaga
     1621 tcggcatctt gagcatcagc cggtccatgg tcttctgcat cttctccgag cgcttccagg
     1681 actggaagaa gaagcacagc ccgccgccga tggagccgaa gatggcccac cagtacttga
     1741 cgaagatctc cgacatcgcc atcacggcca gtgtcggcgc tggcaggtcg gcaccgaagg
     1801 aagtgaacac ctccttgaag gccgggatca cgaagatcat gatgaccgac acgacgacga
     1861 aggccaccac cagcaccgcc accgggtaga tcagtgccga cttgatcttc gacttgatgg
     1921 ccagcgtctt ctcctggtaa gtcgccagcc ggtcaagcag ggtttccagg atacccgccg
     1981 cctcgccagc ctcgatcagg ttgcaataca gcgcgtcgaa gtacatcggg aacttgcgga
     2041 aggcggccga caggctggtc cccgtttcca catcggcgcg gatgtcggtg atcagcttgg
     2101 tcatgcgcgg attggggttg ccgcgcgcca cgatgtcgaa ggcctgcagc aagggcacgc
     2161 ctgc
//
LOCUS       DASUCY010000082         2725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_178471, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000082 DASUCY010000000
VERSION     DASUCY010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178471"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VFV25_01675"
     CDS             <1..426
                     /locus_tag="VFV25_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEY1392053.1"
                     /translation="KKYLDFSMAFRMDETLALLVDIRASQLNGCAFCLDMHVKQASIH
                     GERELRLHHVAIWRESPLFSPKERAALAWTEALTRIAPEGVSDALYAEIREQFSDEEL
                     SMLTFRVTAINGWNRMNVAFRTTPGSQDKMLGLDKAELR"
     gene            complement(431..730)
                     /locus_tag="VFV25_01680"
     CDS             complement(431..730)
                     /locus_tag="VFV25_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392054.1"
                     /translation="MKQRLMWIAWPAFLMAGVLEMVVFALVDPQTLRWFGDTLELSRS
                     AVYTLGFFLFWGATGLSSALTTLLAASPFEVNRCPLPIDGRPQACPKPRPSSDAC"
     gene            complement(751..1050)
                     /locus_tag="VFV25_01685"
     CDS             complement(751..1050)
                     /locus_tag="VFV25_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEY1392055.1"
                     /translation="MMTTQALPTPLRPWWREPLVWMVIGGPAVVVVASFVTLGLVLAR
                     PDPLVSENYYQEGLEYNKTAHPPAEDVASNDAHRPALDARNHAASPRALREGRPH"
     gene            complement(1077..2555)
                     /gene="ccoG"
                     /locus_tag="VFV25_01690"
     CDS             complement(1077..2555)
                     /gene="ccoG"
                     /locus_tag="VFV25_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HEY1392056.1"
                     /translation="MNSKPNVIPILPVTPEAAETASDDDAAGQVVSLYEKQKRIYPRS
                     VHGWFAGWRWSLVWLTQAVFYGLPWLAWNDRQAVLFDLASRRFYIFGLVLYPQDFIYL
                     TALLVLSALGLFLFTAVAGRLWCGYACPQTVYTEIFMWVERHIEGDRIARMRLDAAPW
                     SRHKLARKTAKQAAWIAIGLWTGFTFVGYFTPIHTLAREAATLGLGPWETFWVLFYGF
                     ATYGNAGYMREQVCKYMCPYARFQSAMFDRDTMIITYDERRGEARGSRPRKADPAQLG
                     LGDCIDCRLCVQVCPTGIDIRDGLQYDCIGCAACIDVCDGVMDKMGYARGLIRYSTEN
                     ALEQGWSRGQILRHVLRPRVLIYGGILLAVAGLFVASVLLRSPFRVDVIRDRTSLARL
                     VEGGRIENVYRLQIMSSAESEQRYRISVGGLPGLAIASTPEVEVEPAQARSLAVRVQL
                     PPDEALKLAGRSHPIMFHVEREADAEGQARVVNEKSTFLVPR"
BASE COUNT          458 a          914 c          930 g          423 t
ORIGIN      
        1 aagaagtacc tcgacttcag catggccttc cgcatggacg agacgctggc cctgttggtg
       61 gacatccgcg cctcgcagct caacggctgc gcgttctgcc tggacatgca cgtcaagcag
      121 gccagcatcc acggcgagcg cgagctgcgc ctgcaccacg tggccatctg gcgcgagtcg
      181 ccgctgttct cgccgaagga gcgcgcggca ctcgcctgga ccgaagcgct gacgcgcatc
      241 gcgcccgagg gcgtatcgga cgcgctctat gccgaaatac gcgagcagtt ctccgatgaa
      301 gagctgtcga tgctcacctt ccgcgtcacc gcgatcaatg gctggaaccg catgaacgtg
      361 gccttccgca ccacgccggg atcgcaggac aagatgttgg ggctggacaa ggccgaactg
      421 cgctgaggac tcagcaagcg tccgaactcg gcctcggctt ggggcaggcc tgcggccgcc
      481 cgtcgatcgg cagcgggcag cggttgactt cgaagggcga cgcggccagg agcgtcgtga
      541 gcgcgctgga caggccggtc gccccccaga acaggaagaa gcccagcgtg tagacggccg
      601 agcgcgaaag ctccagcgtg tcgccgaacc agcgcagtgt ttgcgggtcg acgagggcga
      661 acaccaccat ctccagcacg ccggccatca ggaaggccgg ccaggcaatc cacatcaggc
      721 gctgcttcat gggctgctcc ttgcttatgt tcagtgcggc cggccctcgc gcagcgcgcg
      781 cggcgaggcc gcgtgattgc gcgcatcgag cgccgggcgg tgagcatcgt tggaagccac
      841 gtcctcggcc ggagggtgtg ccgtcttgtt gtactccagg ccttcctggt aatagttctc
      901 cgacaccagg gggtcgggcc gcgccagcac cagccccagg gtcacgaagc tggccacgac
      961 gacgacggcc ggcccgccga tcaccatcca caccaagggt tcgcgccacc agggccgcag
     1021 cggggtcggc agggcttgcg tcgtcatcat gatggggctc cttgcttgca gtgatctcaa
     1081 cgtggcacca ggaaggtgga cttctcgttc accacccgcg cctggccttc ggcatcggct
     1141 tcgcgctcga cgtggaacat gatcgggtgc gagcgcccgg ccagcttgag cgcctcgtcg
     1201 ggcggcagct gcacgcgcac cgccagcgag cgtgcctgcg ccggctcgac ttcgacctcg
     1261 ggcgtggagg cgatcgctag ccccggcagc ccgccgacgc tgatgcggta gcgctgctcg
     1321 gattcggcgc tgctcatgat ctgcaggcga tagacgttct cgatgcggcc gccttccacc
     1381 aggcgggcga ggctggtgcg gtcgcggatc acgtcgaccc ggaaggggct gcgcagcagc
     1441 acgctggcga cgaagaggcc tgctacggcc agcaggatgc cgccgtagat cagcacgcgc
     1501 ggcctcagca cgtggcgcag gatctggccg cggctccagc cctgctccaa ggcgttctcg
     1561 gtggagtagc ggatcaggcc gcgcgcatag cccatcttgt ccatcacgcc gtcgcagacg
     1621 tcgatgcagg ccgcgcagcc tatgcagtcg tattgcagcc cgtcgcggat gtcgatgccg
     1681 gtggggcaga cctgcacgca caacctgcag tcgatgcaat cacccaggcc tagctgggcg
     1741 gggtcggcct tgcgcgggcg cgagccgcgc gcctcgccgc gccgctcgtc gtaggtgatg
     1801 atcatcgtgt cccggtcgaa catcgcgctc tggaagcgcg catagggaca catgtatttg
     1861 cacacctgct cgcgcatgta gccggcattg ccataggtcg cgaagccgta gaacagcacc
     1921 cagaaagttt cccacggccc caggcccagc gtcgccgctt cgcgcgcgag cgtgtggata
     1981 ggcgtgaaat agccgacgaa ggtgaagccc gtccacaggc cgatggcgat ccaggccgcc
     2041 tgcttggccg tcttgcgcgc gagcttgtgc cggctccacg gcgctgcgtc cagccgcatg
     2101 cgggcgatgc ggtcgccttc gatgtggcgc tccacccaca tgaagatctc ggtatagacc
     2161 gtctgcgggc aggcatagcc gcaccacagc cggccggcca ccgcggtgaa gaggaagagc
     2221 ccgagcgcgc tgagcaccag cagcgcggtc aggtagatga agtcctgcgg atacaggacg
     2281 aggccgaaga tgtagaaacg ccgcgaagcc aggtcgaaga gcacggcctg ccggtcgttc
     2341 cacgcgagcc agggcagtcc gtagaagacc gcctgggtga gccagaccag gctccagcgc
     2401 cagcccgcga accagccgtg caccgagcgg ggatagatgc gcttctgctt ctcgtacagc
     2461 gagacgacct gcccggccgc gtcgtcgtcg gaagccgtct cggctgcctc gggcgtgacc
     2521 ggcaagatgg ggatgacatt gggcttgctg ttcatggcac gggggcgcgg caagcgccgc
     2581 gcctcgtcgg ctgctggggt cgtcctattt agtcgcggcc ccgctcgcgt cgctgttagc
     2641 cagctcgctc tggtgcgaca agctccacac gtaagccgcc agcacgcgga tctgtgccgc
     2701 gctgaacttg tcgcgatgcg cgggc
//
LOCUS       DASUCY010000083         3363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1463548, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000083 DASUCY010000000
VERSION     DASUCY010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1463548"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /gene="fusA"
                     /locus_tag="VFV25_01695"
     CDS             <1..283
                     /gene="fusA"
                     /locus_tag="VFV25_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544034.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEY1392057.1"
                     /translation="GPQVLEPVMSVEVLTPADYLGDVIGDLHRRRGLVRGQGQRGNTV
                     VVDARVPLKEMFGYIGSLRALSSGRAQYSMQLDHYDAAPASETAELLQG"
     gene            complement(346..717)
                     /locus_tag="VFV25_01700"
     CDS             complement(346..717)
                     /locus_tag="VFV25_01700"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY1392058.1"
                     /translation="MLDHISLGTHRYAEAVAFYSAVLAPLGLTLQRDTGREASFGTQE
                     RWMFFLYPVPPGESVAAQGMHLAFGAAAHRQVEQVHADALAASATDIFTPRARPDISP
                     TYYGAMFHDLDGHRIEVKTDA"
     gene            complement(763..1734)
                     /locus_tag="VFV25_01705"
     CDS             complement(763..1734)
                     /locus_tag="VFV25_01705"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEY1392059.1"
                     /translation="MHIIDTPHRLSLANIERAAAAIDPLFLNSPQFESEPLSQALGCT
                     VTLKVETMNPLRSFKGRGADFFMYEAAQHLAGRALVCASAGNWGQAMAYACRKRGLPL
                     VVYTATTANPLKLERMRAMGAELRLHGEDFDAAKGEARRFCEESGARFVEDGREAAIA
                     EGAGTIGMELMRLRHGIDTVVLPLGNGALLTGVGRWIKAVAPEVRVIGVCSDGADAMA
                     ASWALGRAVDRPRVDTIADGIAVRRPVPEAVADMPGCVDDLLLVSDAAIEQAMRLLCA
                     KAGLVVEPAGAAGVAAVIEHPPLRRQRVATVLCGSNITAEQQARWLG"
     gene            1733..2305
                     /locus_tag="VFV25_01710"
     CDS             1733..2305
                     /locus_tag="VFV25_01710"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY1392060.1"
                     /translation="MPQFLDAALSLGPHPGPPACGDTAFPAPRLRRNRTMILDALDRR
                     ILALYQHDTRIPAERIGDEVGLSAAAVQRRLKRMREGGVIVAETATLDCRLLGLGVTA
                     IVQVDLVDESARASQAFRAKALARAEVQQCYGVTGEYDFLLVVIVQDLAAYDAFCAAC
                     LLHDANVRSFTTQIVLDAAKRGSGLAVPLE"
     gene            2579..>3363
                     /locus_tag="VFV25_01715"
     CDS             2579..>3363
                     /locus_tag="VFV25_01715"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906657.1"
                     /note="catalyzes the phosphorylation and decarboxylation
                     of oxaloacetate to form phosphoenolpyruvate using GTP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HEY1392061.1"
                     /translation="MNRPVMEGLRLNTPAYVKNARLIAWVAEIAALTEAREVYWCDGS
                     QAEYDRLCEQLVQAGTFKRLNPELRPNSYLACSDPSDVARVEDRTFICSAKKEDAGPT
                     NNWMAPAEMRALLQTGPTALFRGAMRGRTLYVVPFSMGPLGSPIAHIGVELSDSPYVA
                     VNMRIMTRMGRAVLDVLGENGEFVPCVHTVGAPLEPGQKDVSWPCNKTKYIVHYPETH
                     EIWSYGSGYGGNALLGKKCFALRIASTMGREQGWLAEHMLILGV"
BASE COUNT          518 a         1127 c         1158 g          560 t
ORIGIN      
        1 gggaccccag gtgctggagc ccgtgatgag cgtggaggtg ctcacgccgg ccgattacct
       61 gggtgacgtc atcggtgacc tgcatcgcag gcgcggccta gttcgcgggc agggccagcg
      121 cggcaacacg gtggtcgtcg atgcccgggt gccgctgaag gagatgttcg gctacatcgg
      181 cagtctgcgc gcgctctcgt ccgggcgtgc gcagtattcg atgcagctgg accactacga
      241 cgcggcacca gccagcgaga cggccgagct cttgcagggc tgagttgcca atgaggaggg
      301 ggcgtggccc gccgggcctc gcccttcctt tctcaccgcg agccttcagg cgtcggtctt
      361 cacctcgatg cggtgcccgt ccaggtcgtg gaacatcgcg ccgtagtagg tggggctgat
      421 gtccggccgg gcgcgtggcg tgaagatatc agtggccgat gcagcgagcg cgtcggcgtg
      481 aacctgctcg acttggcggt gcgcggcggc gccgaacgcc agatgcatgc cttgtgccgc
      541 gacgctttcg cccggcggca ccgggtagag gaagaacatc caccgctcct gcgtgccgaa
      601 gctagcctcg cggccggtat cgcgctgcag ggtcagcccc agtggggcca gcacggcgct
      661 gtagaaggcg acggcctcgg cataacggtg cgtgccgagc gagatgtgat cgagcatggg
      721 ggactccttt gttttgcttc gaatgagagg gagggaaggt gttcaaccta gccagcgcgc
      781 ctgctgctcg gcagtgatgt tgctgccgca cagcacggtg gccacgcgct gccggcgcag
      841 tggggggtgt tcgatcaccg cggccacgcc ggccgcgccg gccggctcga ccaccagccc
      901 ggccttggcg cacagcagcc gcatcgcctg ctcgatggcc gcgtcgctca ccagcagcag
      961 atcatcgacg cagcccggca tgtcggccac ggcttccggc acggggcggc gcacggcgat
     1021 cccgtcggcg atcgtgtcga ccctgggccg atcgacggcg cggcccagcg cccacgatgc
     1081 ggccatggcg tcggcaccgt cgctgcacac gccgatgacg cggacctcag gagccacggc
     1141 cttgatccag cggcccacgc cggtgagcag cgcgccattg cccaggggca gcaccacggt
     1201 gtcgatgccg tggcgaaggc gcatcagctc catgccgatc gtgccggcgc cctcggcgat
     1261 ggcggcctcg cggccgtctt cgacgaaacg agcgccgctt tcttcgcaga agcgacgcgc
     1321 ctcacccttg gctgcgtcga agtcctcgcc atgcaggcgc agctcggcgc ccatcgcgcg
     1381 catgcgctca agcttgagtg gattggccgt ggtcgcggtg tagaccacca gcggcaggcc
     1441 gcgtttacgg caggcgtagg ccatggcctg gccccagttg cccgccgagg cgcacaccag
     1501 tgcgcggccg gccaggtgct gtgcggcctc gtacatgaag aagtcggcgc cacggccctt
     1561 gaaggagcgc agcgggttca tcgtttcgac cttcagggtc acggtgcagc ccagcgcctg
     1621 cgacagcggc tcgctttcga actgcgggct gttcaggaac aaagggtcga tcgccgcggc
     1681 ggcgcgctcg atgttggcca acgacaggcg atggggtgta tcgataatgt gcatgccgca
     1741 attcttggat gcggccctct cgcttgggcc gcatcctggc ccccccgcct gcggcgacac
     1801 cgcatttccg gcgccgcgcc tgcggcgcaa ccgcacgatg atcctcgacg ccctcgatcg
     1861 gcgaatcctc gccctgtacc agcacgacac gcgcatcccc gccgagcgca ttggcgacga
     1921 ggtcggcttg tcggcggcgg ccgtgcagcg gcggctcaag cggatgcgcg agggcggcgt
     1981 gatcgtcgcc gagaccgcca cgctcgactg ccggctgctc ggcctgggcg tgacggccat
     2041 cgtgcaggtg gacctggtcg acgaatcggc ccgcgcctcg caggctttcc gtgccaaggc
     2101 gctggcgcgt gccgaggtcc agcagtgcta tggcgtgacc ggcgagtacg acttcctgtt
     2161 ggtggtgatc gtgcaggacc tcgcggccta cgacgccttc tgtgcggcct gtctgctgca
     2221 cgatgccaac gtgaggagtt tcacgacgca gatcgtgctg gatgcggcga agcgtggcag
     2281 cgggctggcg gtgccgctgg agtagcgtcg gccacgagcg ggcccttcgc tttgctttga
     2341 ggccggtcgg ctcgcttcgc cgcaacaacc caaggcgtcg agaggcgcct attgccatcc
     2401 gcgcgattaa agcttcttgc atcaagctat gcaaaacgtg catgtaatcg cgctgtaacc
     2461 tttggggcgc ggtcctacac tgaatcggtt tatccgctcg caggatgtcg gcagcgcgtt
     2521 tctccttcgt gacagcgccc tgccgagatc ctgttttcaa cttttggagt ttgctgccat
     2581 gaaccgaccc gtgatggagg gcctgcgcct gaacacgccc gcctatgtca agaacgcacg
     2641 cctgatcgcc tgggtcgccg agatcgcggc gctcaccgag gcgcgtgagg tctattggtg
     2701 cgacggcagc caggccgaat acgaccgcct gtgcgagcag ctggttcagg ccggcacctt
     2761 caagcggctc aaccccgagc tgcgccccaa cagctacctg gcctgcagcg atccgagcga
     2821 cgtggcgcgc gtcgaagacc gcaccttcat ctgctccgcg aagaaagagg acgccggccc
     2881 caccaacaac tggatggcgc cggccgagat gcgcgccctg ctgcagaccg ggccgaccgc
     2941 cctgttccgc ggcgcgatgc gcggccgcac gctttatgta gtgccgttct ctatgggccc
     3001 gctgggctcg ccgatcgcgc acatcggcgt ggagctgtcc gacagcccct atgtcgcggt
     3061 caacatgcgc atcatgaccc gcatgggccg cgccgtgctc gacgtgctgg gcgagaacgg
     3121 cgagttcgtg ccctgcgtgc acacggtggg tgcgccgctc gaaccaggcc agaaggacgt
     3181 gagctggccc tgcaacaaga ccaagtacat cgttcactac cccgagacgc acgagatctg
     3241 gagctatggc tcgggctacg gcggcaacgc gctgctgggc aagaagtgct tcgcgctgcg
     3301 catcgcctcc accatgggcc gcgagcaggg ttggctggcc gagcacatgc tgatcctcgg
     3361 cgt
//
LOCUS       DASUCY010000084         2872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1029825, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000084 DASUCY010000000
VERSION     DASUCY010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1029825"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(309..527)
                     /locus_tag="VFV25_01720"
     CDS             complement(309..527)
                     /locus_tag="VFV25_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEY1392062.1"
                     /translation="MKTPRIVTCPTCSGPSVYSPDNPYRPFCSERCKNIDFGAWASES
                     YRVAAQPDPAAEPDTRDGLPGSASAQQH"
     gene            complement(533..1288)
                     /gene="zapD"
                     /locus_tag="VFV25_01725"
     CDS             complement(533..1288)
                     /gene="zapD"
                     /locus_tag="VFV25_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HEY1392063.1"
                     /translation="MILYEYPFNESIRTMLRLEHLFDRLGQLIPRDTAVDHHYALATL
                     FEIMDVASRADLKSDLLKELERLRSQYNGYRGNPSVSEGTLDAVVTRIDHAFDGLHQL
                     PGKAGHALTTNEWLMSIRSRISIPGGTCEFDLPGYYAWQHESAERRRRDLQHWTATMM
                     PLAEALKLLLSLLRDSGSPHMAVAPQGQYQQSLPAGRSYHLLRLKIDPTLGLVPEISG
                     HRLMVLIRLMRQDADGRLRNANEDASFELTLCS"
     gene            complement(1349..1945)
                     /gene="coaE"
                     /locus_tag="VFV25_01730"
     CDS             complement(1349..1945)
                     /gene="coaE"
                     /locus_tag="VFV25_01730"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345584.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEY1392064.1"
                     /translation="MQTRIGLTGGIGSGKSVVATMLADLGACLVDTDAIARQITAPGG
                     AAIPALRAQFGAAVIDAHGGLDRERMRALAFTRPETRRLLESLLHPVIGQEAERQAAA
                     AAPGRPVVFDIPLLVESGRWRARVDRVLVIDCETETQIARVMQRSGWERAAVERVIAQ
                     QARREQRRAAADAVICNEGLDMQGLRQAVNELWRCWRL"
     gene            complement(1964..2821)
                     /locus_tag="VFV25_01735"
     CDS             complement(1964..2821)
                     /locus_tag="VFV25_01735"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEY1392065.1"
                     /translation="MGAPELLGALLSWPALGLIGLCIGSFLNVVIHRLPLMLERQWRA
                     ESAELLGVRAKDEGEPLSLSRPRSRCPSCGHQIRWYENIPLASWLVLRARCSACKTPI
                     SVRYPLFELLTGLLFAAVGWHYGAQPQTLLWCGFTATLVALAAIDWDTTLLPDDLTLP
                     LLWAGLIAAALGWTLPLGTAIWGAVAGYLSLWAVYWLFKLTTGKEGMGRGDFKLLAAL
                     GAWLGWQMLVPILLAASVIGAVVGLAMKLSAQLREGRYVPFGPFLAGAGLVVMLAGAP
                     RVVDWLGWA"
BASE COUNT          457 a          947 c         1008 g          460 t
ORIGIN      
        1 caggaaggta gtgcatcatc gtccatgccg ctgctcgcat tgctgccgat gtgcctgacc
       61 ggattgctga atatcacggc gaactcctta ctgcgctgat catcttccct gtggcgccgc
      121 ctcttgaatc cggctcaagg cctgcgtgcg gcgtggccgt agtgtcctcc cgcgcaatat
      181 ggctgcccat accttaggtc ggtctttccc cgcgaacgag ggggcgcatt ctccttgtaa
      241 agacgtcgat gcgtgaaatc ctcaccggcg ttgctctcag gcgacgcagc cggcgggagg
      301 ccgcggcgtc aatgctgctg cgctgatgcc gagcccggca ggccgtcccg ggtgtcgggc
      361 tcggcggccg ggtcgggttg ggcggcgacg cgatagctct cgctggccca ggcgccgaag
      421 tcgatgttct tgcagcgctc gctgcagaac ggccggtagg ggttgtcggg cgagtagacg
      481 ctgggtccac tacaggtggg gcaggtgacg atgcggggag tcttcatggt cctcagctgc
      541 acagcgtgag ctcgaagctc gcatcctcgt tggcattgcg caggcggccg tcggcatcct
      601 ggcgcatcag gcggatcagc accatcaggc gatggccgct gatttccggc acgagcccga
      661 gcgtcgggtc gatcttgagc ctgagcagat ggtagcttcg cccggccggc aggctctgct
      721 gatactggcc ttgcggcgcg accgccatgt gcggactgcc cgagtcgcgc agcaggctca
      781 gcagcagttt gagcgcttcg gccaggggca tcatcgtggc ggtccagtgc tgcagatctc
      841 ggcggcggcg ctcggcgctc tcgtgctgcc aggcgtagta gcccggcagg tcgaactcgc
      901 aggtgccgcc ggggatgctg atgcggctgc gtatgctcat cagccattcg ttggtggtca
      961 gcgcatggcc agccttgccc ggcagctgat ggaggccgtc gaaggcgtga tcgatgcgag
     1021 tgacaacggc atccagcgtg ccttcggaga ccgaggggtt gccgcggtag ccgttgtact
     1081 ggctgcgcag acgctccagc tccttcagca gatcgctctt gagatcggcg cgcgaggcga
     1141 cgtccatgat ctcgaacagg gtcgccagcg cgtagtggtg gtccaccgcc gtgtcgcggg
     1201 gtatcagctg gccgaggcga tcgaacaggt gttcgaggcg gagcatcgtg cggatgctct
     1261 cgttgaaagg gtattcgtag aggatcaagg cgctcgctcg ctggtgccgg cccgcttgcg
     1321 ttgcgcgggc gcggcgcggt cgattgtttc acaggcgcca gcagcgccac aactcgttca
     1381 cggcctgtcg cagcccttgc atgtccagac cctcgttgca gatcaccgca tcggcggcgg
     1441 cgcggcgctg ctctcgcctc gcctgctggg cgatgacgcg ctcgacggcg gcgcgctccc
     1501 agcccgagcg ctgcattacg cgcgcgatct gggtctcggt ttcgcaatcg atcaccagga
     1561 cgcggtccac gcgagcgcgc cagcggccgg attcgaccag cagcgggatg tcgaatacca
     1621 ccggccggcc gggcgctgcg gcggcggcct ggcgctcggc ttcctggccg atcaccggat
     1681 gcagcaggct ctccagcagg cggcgcgtct cgggccgggt gaaggccagg gctcgcatgc
     1741 gctcgcggtc cagcccgccg tgtgcgtcga tgacggcggc cccgaattga gcgcgcaggg
     1801 cgggaatcgc agccccgccc ggcgccgtga tctggcgggc gatggcatcg gtgtcgacca
     1861 aacaggcgcc gaggtcggcc aacatggtgg cgaccacgct cttaccgctg cctatgccgc
     1921 ctgtgaggcc tatgcgtgtc tgcatggcct caatgatcga ccatcaggcc cagcccagcc
     1981 agtccactac gcgcggcgca ccggccagca tcacgaccag gccggcgccg gcgaggaagg
     2041 ggccaaaggg cacatagcgg ccttcacgca gctgggcgct gagcttcatc gcgaggccca
     2101 cgacagcacc gatcaccgag gccgccagca ggatgggcac cagcatctgc cagcccagcc
     2161 aggcgccgag tgcggcgagc agcttgaaat cgccgcgccc catgccctcc ttgccggtgg
     2221 tcagcttgaa aagccagtag accgcccaca gcgagaggta gccggccacg gcgccccaga
     2281 ttgcggtgcc gagcggcaag gtccagccca gggccgcggc aatgaggccg gcccaaagca
     2341 gcggcagggt gagatcgtcg ggcagcagcg tcgtgtccca gtcgatggcg gccagggcca
     2401 ccagcgtggc cgtaaagccg caccacagca gtgtttgcgg ctgggcgccg tagtgccagc
     2461 cgacggcagc gaacagcagc cccgtcagca actcgaacag cgggtagcgc accgagatgg
     2521 gcgtcttgca ggccgagcag cgggcgcgca gcaccagcca gctcgcgagc gggatgtttt
     2581 cgtaccagcg gatctggtgg ccgcaggacg ggcagcgcga gcgcggccgc gacaggctca
     2641 agggctcgcc ctcgtccttg gcccgcacgc ccagcagctc ggcgctctcg gcccgccatt
     2701 ggcgttccag catcagcggc agccggtgaa tcacgacgtt gaggaagctg ccgatgcaca
     2761 ggccgatcag gcccaacgcc ggccaggaga gcagggctcc cagtagttcg ggcgctccca
     2821 cgctcagacc acttggccga gcttgaagat gggcaggtac atcgacacga cg
//
LOCUS       DASUCY010000085         2450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_676387, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000085 DASUCY010000000
VERSION     DASUCY010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676387"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1447
                     /locus_tag="VFV25_01740"
     CDS             <1..1447
                     /locus_tag="VFV25_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HEY1392066.1"
                     /translation="KTIRLGGVDCGASYLHEPGDARDGVTVTVPIYALNQVSEERCEW
                     LVPGMLKDKVLALVKSLHQKPRARLVPLPDYVAEFVELTPFAQGGLLDVLLKSVRDRT
                     QLAVQRQDFKLEMLAPHLFMNFRVVDEHGRQLGMGRNLAALKAELGGQARSAFQALAA
                     LKTGLPAAAPAAQPPARPADAAGGKGASGGAKAPAKGQGTPVYTAWTFGELPELMEVQ
                     RGAQTLIGFPALIDKLTHVEIEVFDEPDVAAAKHRAGLRRLVALQLKEPLKYLEKNIP
                     DLQKMAVAYMALGTQEELRDQIIDVALERAFLGEPLPTDERSFKARIDEGRGRLNLIA
                     QEVARLAGAVLTEYAAAARKLKDSKPPKEVADDVAAQLQRLMPKRFIAQAAYLQLQHY
                     PRYLKAIVLRLDKLRADPVRDAQRLVELRPLEQRYVRRLAERKGVADARLDEFRWLLE
                     ELRVSLFAQELRTPQPVSVKRLEKAWAQLSS"
     gene            1503..2027
                     /locus_tag="VFV25_01745"
     CDS             1503..2027
                     /locus_tag="VFV25_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392067.1"
                     /translation="MAKPAASTTTAMPLGRMELERVAQRCRKLATKRALLAAGVAAVP
                     VPGIDWVTDVGIMVKLIPQINEAFGLTPQQIERLSPDRRVYVYKAISTAGGWLIGRLV
                     TRELVINVLRLVGVRLTAQQAAKYVPIAGQAVSAALTFSALKLLCEQHIRQCIEVATA
                     LAELPAPEAAASVG"
     gene            2054..2130
                     /locus_tag="VFV25_01750"
     tRNA            2054..2130
                     /locus_tag="VFV25_01750"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2088..2090,aa:Pro,seq:tgg)
     gene            2169..2245
                     /locus_tag="VFV25_01755"
     tRNA            2169..2245
                     /locus_tag="VFV25_01755"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2203..2205,aa:Arg,seq:tct)
BASE COUNT          426 a          810 c          839 g          375 t
ORIGIN      
        1 gaagaccatc cgcctgggcg gcgtggactg cggcgcgagc tatctgcacg agcccggcga
       61 cgcgagggac ggcgtgaccg tcaccgtgcc gatctacgcg ctcaaccagg tgagcgagga
      121 gcgctgcgag tggctggtgc ccggcatgct gaaggacaag gtgcttgcat tggtgaagag
      181 cctgcaccag aagccgcgcg cgcggctcgt gccgttaccc gactacgtgg ccgagttcgt
      241 cgagctcacg ccgttcgcgc agggcggcct gctggatgtg ctactgaaat cggtgcgaga
      301 tcgcacgcaa cttgccgtgc agcgccagga tttcaagctg gaaatgctgg cgccgcacct
      361 gttcatgaat ttccgcgtcg tcgatgagca tggccgccag ctcggcatgg gccgcaacct
      421 cgccgcgctg aaggcggagc tgggcgggca ggcgcgcagc gccttccagg cgctggccgc
      481 gctgaagacc ggcctgccgg ccgccgcgcc ggccgcgcag ccgccggcgc gcccggccga
      541 tgcagcgggt ggcaagggcg cgtcgggcgg tgccaaggcc cctgcgaaag ggcaaggcac
      601 gccggtctac accgcatgga ccttcggcga gctgcctgag ctgatggagg tgcaacgtgg
      661 cgcgcagacg ctgatcggct tcccggcgtt gatcgacaag ctcacgcatg tggagatcga
      721 ggtgttcgac gagcccgacg tcgcggcggc caaacaccgt gccggcctgc gccgcctggt
      781 cgcgctgcag ctcaaggagc ccctcaaata cctcgagaag aacattcccg acctgcagaa
      841 gatggccgtg gcctacatgg cgctgggtac gcaagaggaa ctacgcgacc agatcatcga
      901 cgtggcgctg gagcgcgcct tcctcggcga gccgctgccg accgacgagc gcagcttcaa
      961 ggcgcgcatc gacgaaggcc gtggcagact caacctcatt gcgcaggagg tggcgcgcct
     1021 ggctggtgcc gtgctgaccg aatacgccgc ggcggcccgc aagctgaagg acagcaagcc
     1081 gccgaaagag gttgccgacg acgttgccgc gcagctgcag cgcctgatgc cgaagcgctt
     1141 catcgcgcag gcggcttacc tgcaactgca gcattacccg cgctacctca aggccatagt
     1201 cctgaggctg gacaagctgc gcgccgatcc ggtgcgcgat gcccagcgcc tggtcgagct
     1261 tcgcccgctg gagcagcgct atgtgcgccg cctagcggag cgcaaaggcg tggccgatgc
     1321 gcggctcgac gagttccgct ggctgctcga agagctgcgc gtgagcctct tcgcgcagga
     1381 actgcgcacg ccgcagccgg tgtcggtgaa gcgactcgag aaggcctggg ctcagctgtc
     1441 gagctgaaga cggcgaagtc gccgggttga ccgcgacgcc cacgcgtgca accatggtcc
     1501 gcatggcaaa gccagccgca tcgacgacca ccgccatgcc cctcgggcgc atggagctcg
     1561 agcgcgtcgc gcagcgctgc cgcaagctgg ccaccaaacg cgcgctgctg gccgccggcg
     1621 tggccgcggt gcccgtgcca ggcatcgact gggtgaccga cgtaggcatc atggtcaagc
     1681 tcatcccgca gatcaacgag gccttcgggc tcacgcccca gcagatcgaa aggctcagcc
     1741 cggatcgtcg cgtctatgtc tacaaggcca tcagcacggc aggcggctgg ctgatcggcc
     1801 ggctggtgac gcgcgaactg gtcatcaacg tgctgcggct cgtcggcgtg cggctgacag
     1861 cgcagcaggc agccaaatac gtgcccatcg caggccaggc cgtgtcggct gccttgacct
     1921 tctcggcact caagctgctg tgcgagcagc acatccgcca gtgcatcgag gtcgcaacgg
     1981 ctctggctga gctgccggcg cccgaggccg ccgccagcgt cggctgaaca tcgccggcta
     2041 caattcgaag cttcggggtg tagcgcagcc tggtagcgca actgctttgg gagcagtggg
     2101 tcggacgttc gaatcgtctc accccgacca atcctcttcg gcctgaaccg cctccaccca
     2161 ggccctgact gcccgtagct caactggata gagcagctgc cttctaagca gcaggtcggg
     2221 ggttcgagtc cctccgggca ggccacccaa gtgcctgtcg cgcccgaagg ttccgccgtg
     2281 accgcgaggt tcatggagcg ggtcaggccc tgccttcatg agaagcgagc gccttggtca
     2341 tggtgtattt ggccgcgccg tgcgtgaagc cttgaatctc ataggtgaca gcatagccgt
     2401 gcgccttgta gaacgcgggc gcttgaaagg agaaggtgtc caggtacacg
//
LOCUS       DASUCY010000086         3150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_226651, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000086 DASUCY010000000
VERSION     DASUCY010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226651"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1786)
                     /locus_tag="VFV25_01760"
     CDS             complement(<1..1786)
                     /locus_tag="VFV25_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEY1392068.1"
                     /translation="MTRQTLQQAFPAPLMVGERDADLPVWPIFKQDATATVLVGYAFE
                     SIDFAPVPGFSGTPMNLLVMLDPKGQFIDVRVLSHHEPVFLDGLGEAPLLRFVEQYKG
                     LSLMQNIKIGSNLTRADKNGSVNVYIDGVAKATASVRILNQSLLSAALKVARAKLGYA
                     AGHDPDLVGHVRTDIYTPMDWGAMLRQGLVRRLAVSRREVERAFAGTVGEGQTPAGDG
                     GFVDLYLAYLSVPSVGRNLLSEKGWQHLLNRIDANDHALLVVTRGDYSFVGENFVRGA
                     VPDRLTLHQGELPLELRDLDLDEALRLPPELSLADAKVFRVIGPAGLDPAQPLDLALR
                     VTRSKGMIYPERASRDFTLRYQLPVDYVQASSSDNKSWRSIWIGRAWELALIAVALAL
                     LSVALARQSWLGANERRLARFRTVYLVFTLVFIGWYAQGQLSIVNLTALLQALIAKRK
                     LDFFLYDPVSLVLWAFVAATLVIWGRGTFCGWLCPFGALQELVSQIAQRLGIARIKLH
                     RRLDARLKLAKYAVLAAILATAAVSGPWTDRVVEFEPFKTAITLAFVRSWPFVLWAGS
                     VVLLSAFVYKGYCRYLCPLGAWLALLGRL"
     gene            complement(1882..3078)
                     /gene="pqqE"
                     /locus_tag="VFV25_01765"
     CDS             complement(1882..3078)
                     /gene="pqqE"
                     /locus_tag="VFV25_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018149 - peptide cross-linking [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="HEY1392069.1"
                     /translation="MDAVGSPEPSNRPGPPLWLLAELTHRCPLHCVFCSNPVDYASRR
                     EELDTRDWLNVLDQGRALGAVQLGLSGGEPLLRDDLEEIVAHAHQLGYYINLITSGVG
                     LTEQRATALKKAGLDHIQLSFQDSSRELNDFLSSTRTFELKSRVAAIIKSLGYPMVLN
                     CVMHRYNLPHVGRIIAMAEAMGAEFLELANTQYYGWAWLNRDALMPTPTELADAEAIV
                     DCHRERLAGRMRILWVSPDYVDAKPKACMNGWGSVFLLVSPDGIALPCHSARMLPGLD
                     PPSVLERPLREIWYDSSAFNRYRGSAWMSETCRSCDEHERDFGGCRCQAFLLTGSAEA
                     TDPVCPKSPQRPLVDGLVAQAAARPAAVQTIKLVPNAARQGSLIYRTDANSRQLSSRA
                     AAEHDA"
BASE COUNT          529 a         1015 c         1130 g          476 t
ORIGIN      
        1 gcagccggcc gagcaaggcc agccatgcgc ctagcggaca caggtagcgg caatagccct
       61 tgtagacgaa ggcgctgagt agcacgacgg agcctgccca cagcacgaag ggccaggagc
      121 gcacgaaggc cagggtgatg gccgtcttga agggctcgaa ctcgacgacc cggtcggtcc
      181 agggccccga gaccgcggcc gtcgcgagga tcgcggccag cacagcgtac ttggcgagct
      241 tgagccgcgc gtccagcctg cggtgcagct tgatccgcgc gatgcccagg cgctgtgcga
      301 tctggctcac cagctcctgc agggcgccga aggggcagag ccagccgcag aacgtgccgc
      361 ggccccagat caccagcgtc gccgccacga aggcccacag caccaggctc accgggtcgt
      421 agaggaagaa gtcgagcttg cgcttggcga tcagggcctg cagcagcgcg gtcaggttga
      481 cgatggagag ctgcccctgg gcataccagc cgatgaagac cagcgtgaag accagataga
      541 cggtgcgaaa gcgcgccagc cgccgctcgt tcgcgccgag ccaggattgc cttgcgaggg
      601 cgacgctcag cagtgccagc gcgacggcga tcagcgcgag ctcccaggcg cggccgatcc
      661 agatgctgcg ccaggacttg ttgtcgctgc tcgacgcctg cacgtagtcg accggcagct
      721 ggtagcgcag cgtgaagtcg cggctggcgc gctcggggta gatcatgccc ttgctgcgcg
      781 tcacgcgcag cgcaagatcc agcggctgcg cggggtccag cccggccggg ccgatgacgc
      841 ggaagacctt ggcatcggcg aggctcaact cgggcggcag cctcagcgct tcgtcgagat
      901 cgaggtcgcg cagctccagc ggcagctcgc cctggtgcag cgtcaggcga tcgggcaccg
      961 cgccgcgcac gaagttctcg ccgacgaagc tgtagtcgcc gcgggtgacc acgagtagcg
     1021 catggtcgtt ggcgtcgatg cggttgagca ggtgctgcca gcccttttcg ctcaacagat
     1081 tgcgtcccac gctgggcacg ctgagatagg ccaggtagag atcgacgaag ccgccgtcgc
     1141 ctgcgggcgt ctgcccttcg ccgaccgtgc cggcgaaggc gcgctcgacc tcacggcggc
     1201 tcacggccag gcggcgcacc aggccttggc gtagcatcgc gccccagtcc atgggcgtgt
     1261 agatatcggt gcgcacatgg ccgaccaggt ccgggtcgtg tccggccgca tagccgagct
     1321 tggcgcgcgc caccttcagg gctgcggaca gcaggctctg gttgaggatg cgcaccgagg
     1381 ccgtggcctt ggccacgccg tcgatgtaga cgttgacgct gccgttcttg tcggcgcggg
     1441 tgaggttgct gccgatcttg atgttctgca tcagcgacag gcccttgtac tgctcgacaa
     1501 agcgcagcag cggcgcctcg cccagcccgt cgaggaagac cggctcatgg tgggacagca
     1561 ccctcacatc gatgaactgg cccttgggat cgagcattac cagcagattc atgggcgtgc
     1621 cggagaagcc gggcaccggc gcgaagtcga tcgattcgaa ggcgtagccg accagcacgg
     1681 tcgccgtggc gtcctgcttg aagatcggcc acaccggcag gtcggcatcc ctttccccga
     1741 ccatcagcgg cgcggggaag gcttgctgca gggtctgtcg cgtcatgacg ccagcctcgg
     1801 ccggtgctgc gcatagcagg atgcccagcg ccaggagcag aagccggaag atgcttcgcg
     1861 tgacaatggc catcgccggc gctaggcgtc gtgctccgct gctgcgcgtg aagacaactg
     1921 ccgcgaattc gcatcggtgc gatagatcag gctgccctgg cgggccgcgt tgggcaccag
     1981 cttgatggtc tgcaccgcgg cgggacgcgc ggcggcctgc gcgacgaggc catccaccag
     2041 cggccgctgc gggctcttgg ggcagaccgg gtcggtcgcc tcggccgagc cggtgagcag
     2101 gaaggcctgg cagcggcagc cgccgaagtc gcgctcatgt tcgtcgcagc tgcggcaggt
     2161 ctcgctcatc caggcgctgc cgcggtagcg gttgaaggct gagctgtcgt accagatctc
     2221 gcgcaaaggg cgctcgagca ccgagggcgg gtccagcccc ggcagcatgc gcgcgctatg
     2281 gcagggcagg gcgatgccgt ccggcgagac gagcaggaag accgagcccc agccgttcat
     2341 gcaggccttg ggcttggcgt cgacatagtc cggcgagacc cacaggatgc gcatgcgccc
     2401 ggccaggcgc tcgcggtggc agtcgacgat ggcctcggca tcggccagct cggtcggtgt
     2461 gggcatcagc gcatcgcggt tgagccaggc ccagccgtag tactgcgtgt tggccagctc
     2521 cagaaactcg gcacccatcg cttcggccat cgcgatgatg cggcccacgt gcggaaggtt
     2581 gtagcggtgc atcacgcagt tgagcaccat ggggtagccc aggctcttga tgatggccgc
     2641 gacgcgggat ttgagctcga aggtgcgcgt ggacgagaga aagtcgttga gctcgcgcga
     2701 ggagtcctgg aaggacagtt ggatgtggtc cagcccggcc ttcttgaggg ccgtggcgcg
     2761 ctgctcggtc aggccgacac ccgaggtgat gaggttgatg tagtagccca gctggtgggc
     2821 atgggcgacg atctcctcga ggtcgtcgcg cagcagcggc tcgccgcccg acaggccgag
     2881 ctgcacggcg cccagtgcgc ggccctggtc cagcacattc agccagtctc gcgtgtcgag
     2941 ttcttcgcgt cggctcgcgt agtcgaccgg gttggagcag aagacgcagt gcagcgggca
     3001 gcgatgagtc agctcggcca gcagccaaag cggcgggccg ggacggttag agggctcagg
     3061 cgagccaacc gcgtccataa gcgtcgctca ggaagtggca gacatcctcg cgcagctcgg
     3121 cgcccgcgta ttcgcgctcc agtccttcga
//
LOCUS       DASUCY010000087         2308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1495862, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000087 DASUCY010000000
VERSION     DASUCY010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1495862"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VFV25_01770"
     CDS             complement(<1..369)
                     /locus_tag="VFV25_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392070.1"
                     /translation="MNAWVLQQAGSDAQALDAQLSACDRQMAQARSAGDGERLRLLTP
                     RRLMLGKALTALLRPVRARHRAALRQLFFGRIGKTTTTLTYQLRCDAVKLGLGWATAN
                     AVLDSALLAWEKSLVLGRAPR"
     gene            complement(588..1118)
                     /locus_tag="VFV25_01775"
     CDS             complement(588..1118)
                     /locus_tag="VFV25_01775"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEY1392071.1"
                     /translation="MWQILRQITRVGIATEAPPAAGDTTLADGRRIQEELLAILGRAL
                     AIRQVDAGSCNGCELEIHALNNPYYNIEGLGIKFVASPRHADMLLVTGPVSRHMAEAL
                     KRTYDATPEPKLVVAVGDCGCSGGIFGESYATCGKVADVIPVDLTVPGCPPAPIEILR
                     GVLTAVRRRPLNARRR"
     gene            complement(1121..>2308)
                     /locus_tag="VFV25_01780"
     CDS             complement(1121..>2308)
                     /locus_tag="VFV25_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni,Fe-hydrogenase III large subunit"
                     /protein_id="HEY1392072.1"
                     /translation="LWDLSGAPLRQGSRTQSGPPARLGVEYPFVRVEGDGVHEIAVGP
                     VHAGIIEPGHFRFSVVGEKVLRLEQRLGYVHKGIEQRFTEIAPMDAYRLAGRVSGDST
                     VAYAWAYCMALEAATGPTVPERAQWLRAVLLERERVANHLGDLGALGNDGGFAVGLAQ
                     FSRLREDWLRLSAALFGHRLMMDRIVPGGVAVDLDAAACARLIAQCDALEPELRRLRS
                     IYDEHAGLQDRFIAAGRVTPQLAAQLGLTGLAGRASAQSWDLRCNPSHPPYDRLDVVM
                     ASHRNGDVAARVAVRFDELFESLRLLRELCRRLPAGPVKAELKPGVAEAIGAGWVEGW
                     RGEVFVALELRDRGRAIRRCHCHDPSWQNWPVLEHAVIGNIVPDFPLINKSFNLSYSG
                     HDL"
BASE COUNT          370 a          799 c          799 g          340 t
ORIGIN      
        1 gcggggtgct cggccgagca ccaaggactt ttcccaggcg agcagtgcgc tgtcgagcac
       61 cgcgttcgcg gttgcccagc ctagcccgag tttgacagcg tcacagcgca gctggtaggt
      121 cagcgtggtc gtcgtcttgc cgatgcggcc gaagaagagt tggcgcagcg ccgcccggtg
      181 cctagcgcgc accgggcgca acagcgctgt caacgccttg ccgagcatca ggcgccgcgg
      241 ggtcaagagg cgcaatcgct ctccatctcc ggctgatcga gcttgtgcca tttggcgatc
      301 acaagcgctc aactgggcat cgagcgcctg tgcatcgctg ccggcctgtt gaaggaccca
      361 ggcgttcatc tccgcatgca cgatctgaat gcagccgacc agtccgttgt agagctcacg
      421 ggcttgggcg atctggtccc gggcgacggc tcatcaaggc gcacgccgta ttcatagacc
      481 acgatctgcg gaggcgcggc aatccagggc tcgatcaccc aaggccgctc gcgcgcggca
      541 tggtcacgct tggatctgcc gtgcagggac tcgtcggcct ccatgcttca gcgacgccgg
      601 gcgttcaacg gtctccggcg caccgcggtc aggacgccgc gcaggatctc gatcggtgct
      661 ggcgggcagc ctgggaccgt caagtccacc gggatcacgt cggcgacctt gccgcaggtc
      721 gcatagctct cgccgaagat gccgccggag cagccgcaat cgccgacggc gacaaccaac
      781 ttgggctcgg gcgtggcgtc gtaggtccgc ttcagggcct cggccatgtg gcgcgacacc
      841 gggccggtga ccagcagcat gtcagcatgg cgagggctgg cgacgaactt gatgcccagg
      901 ccctcgatgt tgtagtaggg gttgttgagc gcgtggatct cgagctcgca gccgttgcag
      961 gagccggcat cgacttggcg gatcgccagc gcccggccca ggatagccag cagttcttcc
     1021 tggatgcgcc gcccgtcggc gagcgtcgta tcgcctgccg ccggcggcgc ctcggtcgcg
     1081 atgccgacgc gggtgatctg gcgcaggatc tgccacatgg ctaaaggtcg tgtcccgagt
     1141 agctgaggtt aaaggacttg ttgatcagcg ggaagtcggg aacgatgttg ccgatcaccg
     1201 catgctcgag cacgggccag ttctgccacg acgggtcgtg acagtggcag cggcgaatgg
     1261 cacggccgcg gtcgcgcagt tcgagcgcga cgaacacctc gccgcgccat ccttccaccc
     1321 agccggcgcc gatcgcctcc gccacgcccg gcttcagctc ggccttcacc ggaccggcgg
     1381 gcagcctgcg gcagagctcg cgcagcaggc gcagggattc gaacagctcg tcgaagcgca
     1441 ccgcgacgcg cgcggcgacg tcgccgttgc gatggctggc catcaccaca tcgagccggt
     1501 cgtaaggcgg atgcgacgga ttgcagcgca ggtcccagga ttgcgcgctg gcgcggcctg
     1561 ccaggccggt gaggccgagc tgggcggcca gctgcggcgt gacgcggccg gcggcgatga
     1621 agcggtcctg caggccggcg tgctcgtcgt agatgctgcg caggcggcgc agctcgggct
     1681 ccagcgcatc gcattgcgcg atcagcctcg cgcaagcggc cgcatcgagg tcgaccgcga
     1741 cgccgccggg cacgatccgg tccatcatca gccggtggcc gaacagcgcg gccgacagcc
     1801 gcagccagtc ctcgcgcaag cgcgagaact gcgccagccc gaccgcgaag ccgccatcgt
     1861 tgccaagcgc gcccaggtcc cccaggtgat tggccacgcg ctcacgctcc agcaggacgg
     1921 cgcgcagcca ctgcgcgcgc tcgggcacgg tagggccggt ggctgcctcc agcgccatgc
     1981 agtaggccca ggcataggcc accgtcgagt cgcccgagac gcggccggcg agccggtagg
     2041 cgtccatggg cgcgatctcg gtgaagcgct gctcgatgcc cttgtgcaca tagcccagcc
     2101 gctgctccag gcgcaggacc ttctcgccga ccaccgagaa gcggaaatgc ccgggctcga
     2161 tgatgcccgc atgcaccgga cccaccgcga tctcgtgcac accgtccccc tcgacccgaa
     2221 cgaacgggta ctcgaccccc aggcgcgcag gcggcccgga ttgagtccgg ctgccttgcc
     2281 ggagcggcgc gccggagaga tcccacag
//
LOCUS       DASUCY010000088         2980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_853227, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000088 DASUCY010000000
VERSION     DASUCY010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_853227"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(218..688)
                     /locus_tag="VFV25_01785"
     CDS             complement(218..688)
                     /locus_tag="VFV25_01785"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEY1392073.1"
                     /translation="MSLNATLIAQLVVFLILVWVTMKFVWPPIVTALDARAKKIADGL
                     AAADKAKADLTTANKRVEEQLAAARNETAQRLADAERLAQSMIEEAKTRASEEGAKIV
                     AAAKAEAEQEAVKARETLREQVAALAVKGAEQILHREVNAGVHAELLNRLKTEL"
     gene            complement(735..983)
                     /gene="atpE"
                     /locus_tag="VFV25_01790"
     CDS             complement(735..983)
                     /gene="atpE"
                     /locus_tag="VFV25_01790"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEY1392074.1"
                     /translation="MEHVLGFVALAAGLIVGLGAIGACIGIGIMGSKYLESAARQPEL
                     MNELQVKMFILAGLIDAAFIIGVGIAMMFAFANPFVLH"
     gene            complement(1056..1952)
                     /gene="atpB"
                     /locus_tag="VFV25_01795"
     CDS             complement(1056..1952)
                     /gene="atpB"
                     /locus_tag="VFV25_01795"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEY1392075.1"
                     /translation="MAAEEHGTTAGEYIGHHLTHLRSHEQSFIADFSIFNYDTLFWSI
                     LLGVVGVFVLWRVAKSATSGVPGRMQAAVEVLLEMVDSQAKAIVHNAQSRKFVGPLAL
                     TVFVWIFLMNSMDFLPLDLLPRIWAGIYGAAGGDEHHAYLRVVPTADLSATLGMSIGV
                     LLICFYYNLKIKGPLGWGHELISAPFGSVKLSANPLTWIGFLFMSVANFAMQVVEFFA
                     KTLSHGMRLFGNMYAGELVFMLIALMGAAGFGSASGIALWLGHVVAGSLWAIFHILIV
                     LLQAFIFMMLTLVYVGQAHDAH"
     gene            complement(2022..2594)
                     /locus_tag="VFV25_01800"
     CDS             complement(2022..2594)
                     /locus_tag="VFV25_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HEY1392076.1"
                     /translation="MHADNALRRNLSGTRLNGPGFHVYFYGFAETDCKEAMRIEQGQL
                     TDGVDDPPFKTLTREEAKALRAKCPPLSPWRVIAAQAAVGLAMAAIWWAFSRRGGAAW
                     SALYGAATVVVPGALLARGLTARLFGGNAGAAVLGFLLWESLKIVAAVAMLVAAVRVV
                     PDLSWPALLVTMIVCMKVNWLALLWRRKVS"
     gene            complement(2612..>2980)
                     /locus_tag="VFV25_01805"
     CDS             complement(2612..>2980)
                     /locus_tag="VFV25_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY1392077.1"
                     /translation="GTLSRRRVFASFAPRQPGQDLDSYGGRPDGAAVDAEGCYWVAMF
                     EGGRLLRLSPRGELVSELRLPVRCPTMPCFGGADLRTLYVTTARDKRPPEELAAQPLA
                     GCVLALRVEVPGLPANFYAA"
BASE COUNT          562 a          991 c          958 g          469 t
ORIGIN      
        1 tggcgcagct gcggatcggc ggccacctgg cccagggccg cgagctgctc gccccaggcc
       61 aagacgtcct gccccgccgc agccttgaag gccgcctcgg catagggacg cgcgatggta
      121 gcgagctcgg ccatgtcagg cctcccgccc ggccgcccga agggaggcct gcgccccctc
      181 ggggggcagc gaaccaaggt gagtgtgggg gctcatctca cagctccgtt ttgaggcggt
      241 tcagcaactc ggcgtgaacg ccggcgttga cctcgcggtg caggatctgc tcggcaccct
      301 tgacggccag cgcagccacc tgctcgcgca gggtctcgcg cgccttgacg gcttcctgct
      361 cggcctcggc cttggcggcg gcaacgatct tggcgccctc ttcgctcgcg cgggtcttgg
      421 cttcctcgat catggactgc gccaggcgct cggcatcggc caggcgctgc gcggtctcgt
      481 tgcgcgccgc ggcgagctgc tcctccacgc gcttgttggc ggtggtcaga tcggccttgg
      541 ccttgtcggc cgcggccagg ccgtcggcga tcttcttggc acgcgcgtcg agcgccgtca
      601 cgatgggcgg ccacacgaat ttcatcgtga cccacaccaa gatgaggaac accaccagct
      661 gtgcaattag ggttgcgttc agactcacgg caacaccttt ctagacaggt tggggaactc
      721 ggctaggccg ccgctcagtg caggacgaag gggttggcga aggcgaacat catcgcgata
      781 ccgacgccga tgatgaaggc cgcgtcgatc agaccggcca ggatgaacat cttcacctgc
      841 agctcgttca tcagctcggg ctggcgggcg gccgactcga ggtatttgct gcccatgatg
      901 ccgatgccga tacaggcacc gatggcgccc agaccaacga tcaggccagc ggccagagcg
      961 acaaaaccca acacgtgttc catgtttgct cctttttgga atgtgagaga gaaagagaaa
     1021 gaaagctaag gggtgacgaa agcggtgccc gttgatcagt gcgcgtcgtg ggcctggccc
     1081 acgtagacca gggtcagcat catgaagatg aaggcctgca gcaagacgat caggatgtgg
     1141 aagatggccc agaggctgcc cgcgaccaca tggcccagcc acagcgcgat gccagaggcg
     1201 gagccgaagc cggcggcgcc catcagcgcg atcagcatga agaccagttc gccggcatac
     1261 atgttgccga acaaacgcat gccgtgcgac agcgtcttgg cgaagaactc gaccacctgc
     1321 atcgcgaagt tggcgacact catgaacagg aagccgatcc aggtcagggg attggccgac
     1381 agcttcaccg agccgaaggg cgcggagatc agctcgtggc cccagcccag cgggcccttg
     1441 atcttgaggt tgtaatagaa gcagatcagc agcacgccga ttgacatgcc cagcgtggcc
     1501 gacaggtccg cggtgggcac cacgcgcaga taggcatgat gctcgtcgcc gccggcggcg
     1561 ccgtagatac cggcccagat gcgcggcagc agatccagcg gcaagaagtc catcgagttc
     1621 atcaggaaga tccagacgaa cacggtcagc gcgagcgggc cgacgaactt gcgcgactgc
     1681 gcgttgtgca cgatggcctt ggcctggctg tcgaccattt ccagcaggac ctcgaccgcc
     1741 gcctgcatgc gccccgggac gcccgaggtc gcgctcttgg cgacgcgcca cagcacgaag
     1801 acgccgacga cgcccagcag gatggaccag aacagggtgt cgtaattgaa gatcgagaaa
     1861 tcggcgatga agctctgttc gtgggagcgc aggtgggtca ggtggtgacc gatgtactca
     1921 ccggcggtgg ttccgtgttc ttcggcggcc atgtcggatt cggctttctg ctttgtctcg
     1981 tcttctttat tgaacccgtt tgcgctgctg cgcagcgcaa actagctcac tttgcgccgc
     2041 cacagcagcg ccaaccagtt cactttcatg cacacgatca tcgtgaccag cagggccggc
     2101 cagctcagat cgggcaccac tctcacggct gccaccagca tggcaacagc cgccacaatc
     2161 ttcaatgact cccagagtaa aaagccgagc accgcagcgc cggcattgcc cccgaagagc
     2221 cttgcggtga gaccgcgcgc caataaggca cccggcacca ccacggtggc ggcgccatac
     2281 agcgccgacc aagccgcccc gccccgccgg ctgaaggccc accagatggc agccatcgcc
     2341 agccccaccg cggcctgggc cgcgatgacc cgccagggcg agagcggcgg gcatttcgcc
     2401 ctcaaggcct tagcttcttc gcgcgtcaga gtcttgaaag gcggatcgtc taccccatct
     2461 gtaagctggc cctgttcgat ccgcatggct tccttacagt cggtttcggc aaaaccatag
     2521 aagtatacgt ggaaacccgg gccattcagc ctggttcctg acaggttacg tcggagggcg
     2581 ttgtccgcat gcaccgccgt ggagcggcgc ctcaagcagc gtagaaattg gccggcagcc
     2641 ccggcacctc gacgcgcagc gcgagcacgc agccggccag cggctgggcg gccaattcct
     2701 cgggcgggcg cttgtcgcgc gcggtcgtga catagagcgt gcgcagatcg gcgccgccga
     2761 agcagggcat ggtggggcag cgcaccggca gcctgagctc gctcaccaat tcaccgcgcg
     2821 gcgacaggcg caacagcctg ccgccctcga acatcgcaac ccaatagcag ccctcggcat
     2881 cgacggccgc cccgtcgggc cgcccgccat agctgtcaag gtcctgcccg ggctgccggg
     2941 gcgcaaagct cgcgaacacg cggcgccggc tcagcgtgcc
//
LOCUS       DASUCY010000089         2526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_66106, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000089 DASUCY010000000
VERSION     DASUCY010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66106"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            522..1816
                     /locus_tag="VFV25_01810"
                     /pseudo
     CDS             522..1816
                     /locus_tag="VFV25_01810"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
     gene            1813..>2526
                     /locus_tag="VFV25_01815"
     CDS             1813..>2526
                     /locus_tag="VFV25_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEY1392078.1"
                     /translation="MKIAAIQMVASPRVEENLAHARQRIAEAAAAGAELVALPEYFCL
                     MGMKDSDKLAAAEAEGEGPIQEALAEAARAHGLWLIGGTLPLRIAGDASHALNTSLVF
                     APDGERAARYDKIHLFRFDDGERRYDEAATLKPGREPRAFELRDRGGQAWRVGLSVCY
                     DLRFPELYRALSRPPCDLIVVPSAFTYPTGQAHWELLLRARAVENQCYVLAPAQGGRH
                     ENGRRTWGHSMLVDPWGEVI"
BASE COUNT          376 a          908 c          913 g          329 t
ORIGIN      
        1 gcctcaggcg ggccggcgaa ctgggcagcc tggccgcgct gggtgcgcat atgagcgggc
       61 agacacccta tcagctcaag ctgggcttcc ccaagggccg caccgagttc agcctcagca
      121 gcagcctggt cgggctgggc ctgaacctgc ccgcgccgct gcgcaagccg gccggcgagg
      181 ccatgccgct gcgcatgcag ctggccgcat tgcccgctga gccctcggcg cccgcgctgg
      241 accggctgag cgtggagctg ggcagcctgc tgcagacgca ataccagcgc gacgtctcgg
      301 gcgagcagcc gcgcgtgctg cgcggcagcc tggcgctggg attggcggcc gacgaggcgg
      361 cgccgctgcc tgtcagcggc gtggccgcca gcgtgaagct gcccagcctc aatgtcgacg
      421 cctggcagaa gctgctcgac gaacttcagc cttcggccga cgacggcaag actgccgccg
      481 cggccgatcc gccggccggg ggcgcctatc tgccgacgca gatggcgctg cgcgcgaagg
      541 agctgatcgt gaacggccgc aagctggaca acgtggtggc cggcatcacc cacgacacgc
      601 ggcgcgagga cagttactgg cgcgccaatc tcgaggccga ccagctcagc ggctatgccg
      661 aataccggca gccgcgccgc ggcgaggaat cggggcgcat ctatgcgcgg ctggcgcggc
      721 tggcgctgcc caggagcgag gtcgaggccg tcgagaacct gctggaacag tcgccgcgca
      781 gcgtgccggc gctggacatc gtcgtcgatg acctcgacct gcgcggcaag cgcctggggc
      841 gcgtcgagat cgaggccacc aaccagcgcg cggccgatgc gcgcgcagac ggcgctgccg
      901 cacgcgagtg gcggctgtcc aagcttaact tcagcacgcc cgaggccaag ttcgccgcca
      961 cgggctactg gagcgcgccg ggcgcggccg aaccgcaggg cggccagggt ggctcgggca
     1021 catcgcgccg ccgcgccgtg atgaacttca agctcgacct ggccgacagc ggtggcttcc
     1081 tcgcgcgcat gggcctgccc ggcacgctgc gcggcggcaa gggcaagatg agcggccagg
     1141 tgtcctggct gggctcgccg ctgtcgcctg actacccgag cctggccggc aacgtcaacc
     1201 tggccatcga gtccgggcag ttcctcaagg ccgatcccgg catcgccaag ctgctgggcg
     1261 tgctgagcct gcagtcgctg ccgcgccggc tgttgttcga cttccgcgat gtcttcctcg
     1321 acggcttcag cttcgacggc gtgaacggcg acgttgcgat cgccaacggc gtggccagca
     1381 cgcgcaacct gcgcatgcgc ggcgtgcagg ccgtggtgct gatgaaggcc aggccgacat
     1441 cgcgcacgag acccaggacc tgcgcgtctg ggtcgtgccc gagatcaacg ccggcgccgc
     1501 ctcgctggcc tatgccgtga tcaacccggc ggtcggcctg ggcaccttcc tcggccagct
     1561 cttcctgcgc aagccgctga tcgaggcgag cacgcgcgag ttcgcggtct cgggctcctg
     1621 ggccgatccc aaggtcgagc gcatcgagcg caaggcgggc gacgccatgc ccaagactga
     1681 gcccgagccc gagccggaag ctgcgcccgc cgctcccgag cctgcggcgt cggagccctc
     1741 ggcacccgct gcggccgagc cggcagcctc cgcggccagc ggcggcgcaa tgagtacagg
     1801 ggagaagacg ccatgaagat cgccgccatc cagatggtcg ccagccccag ggtcgaagag
     1861 aaccttgccc atgcgcggca acgcattgcc gaggccgctg ccgcgggcgc cgagctggtg
     1921 gccctgcccg agtacttctg cctgatgggc atgaaggaca gcgacaagct cgccgccgcc
     1981 gaagccgagg gcgaggggcc gatccaggag gcgctggccg aggcggcacg cgcgcacggc
     2041 ctgtggctga tcggcggcac gctgccgctg cgcatcgcgg gcgacgcgag ccatgcgctc
     2101 aacaccagtc tcgtattcgc gcccgacggc gagcgtgcgg cgcgctacga caagatccat
     2161 ctgttccgct tcgacgacgg cgagcgccgc tatgacgagg ccgccacgct caagcccggc
     2221 cgcgagcccc gggccttcga actgcgcgac cgcggcggcc aggcctggcg cgtgggcctg
     2281 agcgtgtgct acgacctgcg cttccccgag ctgtaccgcg cgctgagccg gccgccctgc
     2341 gacctcatcg tcgtgccctc ggctttcacg tacccgaccg gccaggcgca ttgggagctg
     2401 ctgctgcgcg cccgcgcggt ggagaaccag tgctatgtgc tggcgccggc ccagggcggg
     2461 cggcacgaga acgggcggcg cacctggggc cacagcatgc tggtcgaccc ctggggcgag
     2521 gtgatc
//
LOCUS       DASUCY010000090         2660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1335181, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000090 DASUCY010000000
VERSION     DASUCY010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1335181"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /gene="dapB"
                     /locus_tag="VFV25_01820"
     CDS             <1..810
                     /gene="dapB"
                     /locus_tag="VFV25_01820"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEY1392079.1"
                     /translation="DSATHRVAVAGASGRMGHMLIEAISAADDCGLTGALDQAGSPNL
                     GQDAAGFLGRRSGVAITADLREGLKGAQFLIDFTRPEGTLAHLKACRELGVKLVIGTT
                     GFSEAQKAEIAAAAKDIAIMMAPNMSVGVNVVLKLLATAAKALNQGYDIEIIEAHHRH
                     KVDAPSGTALAMGQAVADALGRDLKQCAVYGREGITGERDPSTIGFATVRGGDIVGDH
                     TVLFAGTGERIEISHKSSSRATYAQGSLRAVRFLAGQEKGLFGMDEVLGLQ"
     gene            807..1484
                     /locus_tag="VFV25_01825"
     CDS             807..1484
                     /locus_tag="VFV25_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEY1392080.1"
                     /translation="MSGFMQFWSRGDAITQAVAALLLAMSVCAWVLIFWKTWLLRRAR
                     RDIGRAVPAFWSATELEAARNQLTAFDRESVVSPLLEAATQAARPNTLEAAGQLESQL
                     TRRLRDALHGVLQQLQFGQVLLASIGSTSPFIGLFGTVWGIYHALASISAAGGITIEK
                     VSGPVGEALIMTAAGLAVAIPAVLAYNLFGKWLGACEAELEGFAHDLREMMLDRVRAS
                     AVRSREA"
     gene            1489..1905
                     /locus_tag="VFV25_01830"
     CDS             1489..1905
                     /locus_tag="VFV25_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY1392081.1"
                     /translation="MAFGRLERSTGPAPMSDINMTPLIDVMLVLLVIFMVTAPLMASS
                     LKLDLPKADAAQPGDTAEFLAVAISADGTLYLSEEKLAQGAFRERVLAAAKRTPDIEV
                     QLRADQAVPYGRVAELIGLMQQAGLSRIAFVTEKAP"
     gene            1989..>2660
                     /locus_tag="VFV25_01835"
     CDS             1989..>2660
                     /locus_tag="VFV25_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY1392082.1"
                     /translation="MSSDPQAYDPRSIEAQAQAHWQAADAYRVVEDASRPKFYACSML
                     PYPSGKLHMGHVRNYTINDMLTRYLRMKGYNVLMPMGWDAFGLPAENAAMKNKVPPAK
                     WTYENIAYMKQQMLAMGLAIDWSRETATCSPDYYRWNQWLFLRMLEAGIAERRTQVVN
                     WDPVDQTVLANEQVIDGRGWRSGAVVEKREIPGYYLKITQYADELLDHVQHHLPGWPE
                     RVRLMQ"
BASE COUNT          448 a          927 c          880 g          405 t
ORIGIN      
        1 gattcggcta cgcaccgcgt cgccgttgcg ggggcatccg gccgcatggg gcacatgctg
       61 atcgaggcga tcagcgcggc agacgactgc gggctgacgg gcgcgcttga ccaggccggc
      121 agccccaatc tgggccagga cgcagcgggc tttctgggcc ggcgtagcgg cgttgctatc
      181 accgctgatc tgcgcgaggg cttgaagggc gcgcagttcc tgatcgactt cacgcgcccc
      241 gaaggcacgc tcgcccatct gaaggcctgc cgcgaactgg gcgtcaagct cgtcatcggc
      301 accaccggct tcagcgaggc gcagaaggcc gagatcgcgg ccgccgccaa ggacatcgcc
      361 atcatgatgg cgcccaacat gagcgtgggc gtcaacgtcg tgctcaagct gctggctacc
      421 gctgccaagg ccctgaacca gggctatgac atcgagatca tcgaggccca tcaccgccac
      481 aaggtcgatg cgcccagcgg caccgcactg gcaatgggcc aagccgtcgc cgacgccctg
      541 ggccgcgacc tcaaacaatg cgccgtctat ggccgcgagg gcatcactgg cgagcgcgat
      601 ccgtccacga tcggcttcgc caccgtgcgc ggcggcgaca tcgtcgggga ccacaccgtg
      661 ctcttcgccg gcaccggcga gcgcatcgag atcagccaca agtccagcag ccgcgccacc
      721 tatgcccagg gcagcttgcg cgcggtgcgc ttcctggccg gccaggaaaa agggctgttc
      781 ggcatggacg aggtgctcgg cctgcaatga gcggcttcat gcagttctgg agccggggcg
      841 acgccatcac gcaggctgtc gcggcgctgc tgcttgcgat gtcggtctgc gcctgggtgc
      901 tgatcttctg gaagacctgg ctattgcgcc gcgcgcgccg cgacatcggc cgcgccgtgc
      961 cggccttctg gtcggcgacg gagctggaag cggcgcgcaa ccagctcacc gccttcgacc
     1021 gcgaaagcgt cgtgagcccg ctgctcgagg cggcgaccca ggccgcccgc cccaatacgc
     1081 tcgaggctgc gggccagctc gaatcccagc tcacacgccg cctgcgcgac gccctgcatg
     1141 gcgtgctgca gcagctgcag ttcggccagg tgttgcttgc ctcgataggc tcgacctcgc
     1201 ccttcattgg cctcttcggc acggtctggg gtatctatca cgcgctggcg agcatctccg
     1261 ccgccggagg catcacgatc gagaaggtct ccggcccggt cggtgaggcc ttgatcatga
     1321 cggccgccgg cctggccgtg gcgatacccg cagtgctggc ctacaacctg ttcggcaaat
     1381 ggctgggcgc ctgcgaggcc gagctcgaag gcttcgcgca cgatctgcgc gagatgatgc
     1441 tggaccgcgt ccgcgcgtcc gcagtccgaa gccgcgaggc ctgaaaccat ggccttcggc
     1501 cgtctcgagc gcagcaccgg ccccgcgccg atgagcgaca tcaacatgac gccgctgatc
     1561 gacgtgatgc tggtgctgct ggtgatcttc atggtcacgg cgccgctgat ggccagcagc
     1621 ctcaagctcg atctgcccaa ggccgatgcc gcccagcccg gcgacacggc ggagttccta
     1681 gcggtggcga tctcggccga cggcacgctc tacttgagcg aagagaagct ggcgcagggt
     1741 gccttccgcg agcgcgtgct ggccgcggcc aagcgtacgc ccgatatcga ggtccagctg
     1801 cgcgccgatc aggcggtgcc ttatggccgc gtcgccgagc tgatcggcct gatgcagcag
     1861 gccggcctga gccgcatcgc cttcgtgacc gagaaggcgc cataagcgcc ggccgggtgc
     1921 gggacgcacc ctaaaatcgc gctcatcccc acatccagac cgagcgggcg cgtcgcgccc
     1981 ctgtgcgcat gagttccgac ccgcaagcct acgatccccg ctccatcgaa gcccaggccc
     2041 aggcgcattg gcaggccgcc gatgcctatc gcgtcgttga ggacgcgagc cgccccaagt
     2101 tctacgcctg ctccatgctg ccctacccca gcggcaagct gcacatgggc catgtgcgca
     2161 actacacgat caacgacatg ttgacgcgtt acctgcgcat gaagggctac aacgtgctga
     2221 tgcccatggg ctgggacgcc ttcggcctgc cggccgagaa cgcggcgatg aagaacaagg
     2281 tgccgccggc gaagtggacc tacgagaaca tcgcctacat gaagcagcag atgctggcga
     2341 tggggctggc catcgactgg agccgcgaga ctgcgacctg ctcgcccgac tactaccgct
     2401 ggaaccagtg gctgttcctg cgcatgctgg aggccggcat cgccgagcgc cgcacccagg
     2461 tggtgaactg ggatccggtg gaccagaccg tgctcgccaa cgagcaggtc atcgacggcc
     2521 gcggctggcg ctcgggcgcc gtggtcgaaa agcgcgagat ccccggctac tacctcaaga
     2581 tcacccagta cgccgacgag ctgctcgacc atgtgcagca ccacctgcca ggctggcccg
     2641 agcgcgtgcg cctgatgcag
//
LOCUS       DASUCY010000091         2520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_580210, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000091 DASUCY010000000
VERSION     DASUCY010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580210"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1674)
                     /gene="hutU"
                     /locus_tag="VFV25_01840"
     CDS             complement(<1..1674)
                     /gene="hutU"
                     /locus_tag="VFV25_01840"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEY1392083.1"
                     /translation="MNAPDNFIVPAADPRHDASRVIRAPRGSQLHCKSWLTEAAFRMI
                     QNNLDAEVAEDPKNLVVYGGIGRAARNWECFDQILASLKELNNDETLLVQSGKPVGVF
                     KTHENAPRVLIANSNLVPKWANWEHFNELDRKGLFMYGQMTAGSWIYIGSQGIVQGTF
                     ETFVEAGRQHYNNSLAGKWILTAGLGGMGGAQPLAATLAGAVSLNIECQQSSIDFRLR
                     TRYVDKQAKDIDDAMALIKQHTAAREAVSIALLGNAAEILPELVKRARAGGIKPDLVT
                     DQTSAHDLINGYLPTGWSVAQWKAAQRDPEQHARLTDAAAKSCAVHVQAMLDFQAMGI
                     PTVDYGNNIRQVAFDMGVKNAFDFPGFVPAYIRPLFCEGKGPFRWVALSGDPEDIYKT
                     DAKIKELFPDNKHTHRWLDMARERIAFQGLPARICWLGLGERHRAGLAFNEMVKNGEL
                     KAPIVIGRDHLDTGSVASPNRETEAMKDGTDAVSDWPLLNALLNTAGGATWVSLHHGG
                     GVGMGYSQHAGMVIVCDGTSSAAKRIERVLWNDPATGVMRHADAGYAEAI"
     gene            complement(1700..>2520)
                     /locus_tag="VFV25_01845"
     CDS             complement(1700..>2520)
                     /locus_tag="VFV25_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HEY1392084.1"
                     /translation="DARIHAARGQPGQIAVAGAVRALLEGSEILPSHADCGRVQDPYS
                     IRCVPQVMGACLDNLAHAARVLCIEANAASDNPLVFTDTGEVISGGNFHAEPVAFAAD
                     IIALAVSEIGAIAERRLALLLDTGLSGLPPFLVRDGGLNSGFMIAQVTAAALASENKS
                     LAHPASVDSLPTSANQEDHVSMATFAARRLGEMVNNTAVVVGIEAMAAAQGIELKRNL
                     KSSPLVEAEFATIRQKVAFLERDRYLAPDIEAMRQWALRAELPAALLNILPSHS"
BASE COUNT          371 a          793 c          892 g          464 t
ORIGIN      
        1 gatcgcttcc gcatagccgg catcggcgtg acgcatcacg ccggtcgcag gatcgttcca
       61 cagcacgcgc tcgatacgct tggccgcgct tgacgtgccg tcgcagacga tgaccatgcc
      121 tgcgtgctgc gaataaccca tgccgacgcc gccgccgtgg tgcaggctga cccaggtggc
      181 gccgcccgcg gtgttgagca gcgcgttgag cagcggccag tcggaaacgg catcggtgcc
      241 gtccttcatg gcttcggtct cgcggttagg gctggccacc gagccggtgt ccaggtggtc
      301 gcggccgatc acgatcgggg ccttgagttc accgttcttc accatttcgt tgaaggccag
      361 accggccctg tggcgctcgc ccaggcccag ccagcagatg cgtgcgggca ggccctggaa
      421 ggcgatgcgc tcgcgcgcca tgtcgagcca gcggtgcgtg tgcttgttgt cggggaacag
      481 ttccttgatt ttggcatcgg tcttgtagat gtcttccggg tcgcccgaga gcgcgaccca
      541 gcgaaacggc cccttgcctt cgcagaacag cggccggatg taggccggca cgaaaccggg
      601 gaagtcgaag gcgttcttca cacccatgtc gaaggcgacc tggcggatgt tgttgccata
      661 gtccacggtg gggatgccca tggcctggaa gtccagcatg gcctgcacgt gcacggcgca
      721 ggacttggcg gcggcgtcgg tcagcctggc gtgctgctcg gggtcgcgtt gcgccgcctt
      781 ccactgcgcc acgctccagc ctgtgggcag gtagccgttg atgagatcgt gggccgaagt
      841 ctggtcggtc accaggtcgg gcttgatgcc gccggccctg gcgcgtttga ccagctcggg
      901 caggatctcg gcggcattgc ccagcagggc gatcgacacg gcctccctgg cggctgtgtg
      961 ctgcttgatg agcgccatcg cgtcatcgat gtccttggcc tgcttgtcga cgtagcgggt
     1021 gcgcaggcga aagtcgatgc tcgactgctg gcactcgatg ttgagcgaca ccgcaccggc
     1081 cagcgtggcg gccagcggct gcgcgccgcc catgccgccc aggcctgccg tcagaatcca
     1141 tttgcccgcc aggctgttgt tgtagtgctg gcggccggct tccacaaaag tttcaaaagt
     1201 gccctggacg atgccctggc tgccgatgta gatccagctg ccggcggtca tctggccgta
     1261 catgaacagg cccttgcggt cgagttcgtt gaaatgctcc cagttggccc acttgggaac
     1321 caggttggaa ttggcgatca gcacgcgcgg cgcgttttcg tgggtcttga acacgccgac
     1381 cggcttgccg gattgcacca gcagcgtctc gtcgttgttc agttccttga gcgaggcgag
     1441 gatctggtca aagcactccc agttgcgcgc ggcacggccg atgccgccgt agaccaccag
     1501 gttcttcggg tcttcggcca cctcggcgtc caggttgttc tggatcatgc ggaaggcggc
     1561 ttcggtcagc cagctcttgc agtggagctg gctgccgcgc ggcgcgcgga tcacgcggct
     1621 cgcgtcatgg cgcgggtcgg ccgcaggcac gatgaaattg tcgggggcgt tcatggcaag
     1681 gtctcctgag aggtacggtt taggaatggc tgggcaggat gttcagcagg gcggcgggca
     1741 gctccgcgcg cagggcccac tggcgcatgg cttcgatgtc gggggccagg tagcggtcgc
     1801 gctcgagaaa cgccaccttt tggcggatcg tggcgaactc ggcttcgacc aggggtgagc
     1861 tcttgaggtt tcttttgagt tcaatgccct gggcggcggc catcgcttcg atgccgacca
     1921 cgacggccgt gttattgacc atctcgccga gccggcgcgc cgcgaaggtg gccatcgaca
     1981 catggtcttc ctggttggcc gaggtcggca ggctgtcgac gctggcgggg tgcgccagcg
     2041 acttgttctc cgaggcgagg gctgcggccg tgacttgcgc gatcatgaag ccggagttga
     2101 gtccgccgtc gcggaccagg aagggcggca ggcccgagag gccggtgtcc agcagcagcg
     2161 cgaggcggcg ctcggcgatc gcgccgattt cgctcacggc cagtgcgatg atgtcggccg
     2221 cgaaggcaac cggctcggcg tggaagttgc cgcccgagat gacctcgccg gtgtcggtga
     2281 acaccagcgg gttgtccgac gcggcgttcg cctcgatgca gagcacccgc gccgcatgcg
     2341 cgagattgtc gaggcaggcg cccatcacct gcggcacgca gcggatcgag tacgggtcct
     2401 gcacccggcc gcagtcggca tgcgagggca ggatctcgct gccttcgagc aaggcgcgca
     2461 ccgcgcccgc caccgcgatc tgccccggct ggccgcgcgc cgcatggatg cgggcgtcga
//
LOCUS       DASUCY010000092         6409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_789067, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000092 DASUCY010000000
VERSION     DASUCY010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 6409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6409
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789067"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..591)
                     /gene="trmL"
                     /locus_tag="VFV25_01850"
     CDS             complement(121..591)
                     /gene="trmL"
                     /locus_tag="VFV25_01850"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEY1392085.1"
                     /translation="MFNIVLVEPEIPPNTGNIIRLAANTGCSLHLIEPLGFSMDDKLL
                     QRAGLDYHEYAPVRRHASWDRFLADCEPDPARLFAFTTHGSRPFAQMAWRPGDWLVFG
                     CETRGLAPALRECFAPEQRVRLPMREGQRSLNLSNTVAIAVFEAWRQNGYAGGR"
     gene            complement(603..1328)
                     /locus_tag="VFV25_01855"
     CDS             complement(603..1328)
                     /locus_tag="VFV25_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEY1392086.1"
                     /translation="MFSRLLHRRPFALRWPSQCAVCHSWQAERLCEPCLRRFVPQTNR
                     CVRCALRLPDGPKLCGECIKQPPPYEFTVAALDYDHPWDGLLHALKFRAALDLAPTLA
                     ARLQHAVETRLQPGIMPRPDLLLPTPLHPARLRERGHNPAWELARRLAAPLGLCAESR
                     LLLRIKDTPHQLALTREQRRANMRDAFALEPARRSEVAQRSVAIVDDVMTSGATAAEM
                     ARTLLQAGASSVQIWVLARTALE"
     gene            1358..2281
                     /locus_tag="VFV25_01860"
     CDS             1358..2281
                     /locus_tag="VFV25_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY1392087.1"
                     /translation="MNAAARDTSPATRPDAGAVQGMLRRLARGTGAPWLHAEIARRMA
                     EKLELIKREPAVVADWWGFLGGGAVHLAQRYPKAQRVVVEPSEALRARSLEQGRQPWW
                     SPRRWRSSATEVRLDAIQPGSAQLLWANMMLHWVDDVPSLFAEWQRSLAVDGFVMFST
                     FGPDTLKELRALYAQLGWPQPAQAFVDMHDIGDQLVHAGFADPVMDMEQLTLSWPEPQ
                     ALLAELRGLGSNLSAARGTGLRTPRWRARLEQELQSLAGADGRLSLSFEIVYGHAFKP
                     APRPRIEGETKVSLEDMRSMVRSRRGGGEGS"
     gene            2499..3683
                     /gene="coxB"
                     /locus_tag="VFV25_01865"
     CDS             2499..3683
                     /gene="coxB"
                     /locus_tag="VFV25_01865"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEY1392088.1"
                     /translation="MKALGLVLRHGAHKAGRLALTLGAGLATTAALAVNDLPGGPAVL
                     QIDLPPAATKIAAEQQWLHYFMLIICLVIFAGVFGVMFYSILKHRKSKGAQSANFHES
                     TTVEIVWTIVPFIIVIAMALPATKAVVAMKDTTNSDLTIKATGYQWKWGYDYLKGEGE
                     GIGFISTLDVNQRVMSNLGQPEGDDYLLKVDNPLVVPVNKKVRIITTAADVIHAFAVP
                     QFGIKQDAIPGFVRDTWFRAEKVGDFYGQCQELCGKEHAYMPIHVKVVSAEDYTKWVE
                     GKKKEIAAASEDPNKVWTQAELIARGEGVYGANCATCHQANGKGGGPIKPLDGSPVVL
                     DADKTKQITVVLHGRQGTAMPPWSQLSDTDLAAVITFTKNHWSNATGQAVQPAEVLAA
                     RK"
     gene            3717..5351
                     /gene="ctaD"
                     /locus_tag="VFV25_01870"
     CDS             3717..5351
                     /gene="ctaD"
                     /locus_tag="VFV25_01870"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEY1392089.1"
                     /translation="MSAVLEPHGHVAGDHAHDDHAHGHPHGWRRWVFATNHKDIGTLY
                     LLFSFTMLLIGGVLALCIRAELFKPGLQFVNPELFNQFTTMHGLIMVFGAIMPAFVGF
                     ANWMVPLQIGASDMAFARMNNFSFWLLIPAALMLVLSFFMPGGAPAAGWTLYAPLTLQ
                     MGPSMDAGIFAMHIMGASSIMGSINIIVTILNMRAPGMTLMRMPLFAWTWLITAYLLI
                     AVMPVLAGAITMTLTDRHFGTSFFNPAGGGDPVMYQHIFWFFGHPEVYIMILPAFGII
                     SAIVPAFARKKLFGYTSMVYATASIAILSFIVWAHHMFTTGMPVTGQLFFMYATMLIA
                     VPTGVKIFNWIATMWKGSMTFETPMLFAVGFIFVFTMGGFTGLILAMAPIDIQLQDTY
                     YVVAHFHYVLVAGSLYAMFAGFYYWSPKWCGVMYNETRGKIHFWGSLITFNVTFFPMH
                     FLGLAGMPRRYADYPMQFTDFNMVASIGAFGFGLMQVYFLLFVVLPVMRGQGEKAPQK
                     PWEGAEGLEWEIPSPAPFHTFETPPKLDSSATRVIG"
     gene            5380..5499
                     /locus_tag="VFV25_01875"
     CDS             5380..5499
                     /locus_tag="VFV25_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome oxidase small assembly protein"
                     /protein_id="HEY1392090.1"
                     /translation="MTTPEEKKKSNLRLALILASVALAFFFGFIAKMSLFAHH"
     gene            5521..6159
                     /locus_tag="VFV25_01880"
     CDS             5521..6159
                     /locus_tag="VFV25_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEY1392091.1"
                     /translation="MSPSGTDRQAAARRAENRRMVGKLVVVAALMFGFGYALVPVYRS
                     ICNALGINVLSLSELAVPGAPGANGVANTQVDRSRTITVEFDANAHGPWDFKSEQRSM
                     QVHPGELATVMYEFRNVQDHTMSAQAIPSYAPKEATEHFHKLQCFCFTQHTLAPGESK
                     RWPVVFVIDPKLPKSVTTITLSYTFFEVGGEMRAAPQSTAGLQQLTKPGAAS"
     gene            6156..6404
                     /locus_tag="VFV25_01885"
     CDS             6156..6404
                     /locus_tag="VFV25_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2970 domain-containing protein"
                     /protein_id="HEY1392092.1"
                     /translation="MSDAPRPGADLKEAAQRKGSFLQTMRAVAWSFFGVRKSKDYEQD
                     VSKLNPVHVIIAGVIGAAIFVVVLVLLVQWVIGSGVAK"
BASE COUNT         1083 a         2166 c         2108 g         1052 t
ORIGIN      
        1 gccgcgaggc caaacccgaa gcctgacggg tgtcgtcaca ggcggcgccg cgccgggccg
       61 ctcccaagca agccggcacc ctcacggagg gcggacgcgg cacgccgcgg ccgggtgttg
      121 tcatctcccg ccggcgtagc cgttttgccg ccaggcctcg aacacggcga tggccacggt
      181 gttgctgaga ttgaggctgc gctggccctc gcgcatgggc aagcgcacgc gctgctccgg
      241 cgcaaagcat tcgcgcagcg ccggcgccag gccgcgcgtc tcgcagccga acaccagcca
      301 gtcgccgggc cgccaggcca tttgcgcaaa gggccggctg ccgtgcgtgg tgaaggcgaa
      361 gaggcgagcc gggtcgggtt cgcagtcggc caggaagcga tcccaggatg cgtggcgccg
      421 caccggtgcg tattcgtggt aatccagtcc ggcgcgctgc agcagcttgt cgtccatcga
      481 gaagcccagg ggctcgatca agtgcaggct gcaacccgtg ttggcggcca gccggatgat
      541 gttgccggtg ttgggcggga tctcgggctc gaccaggacg atgttgaaca tgagggtggc
      601 tctcattcga gggcggtgcg cgccagcacc cagatctgca cgctggatgc gcccgcctgc
      661 agcagcgtgc gtgccatttc ggccgccgtg gcgccgctcg tcatcacgtc gtcgacgatg
      721 gccacgctgc gctgcgcgac ttcgcttcgc cgcgcaggtt cgagtgcgaa ggcatcgcgc
      781 atgttggctc ggcgctgctc gcgcgtcagt gcgagctgat gcggcgtgtc cttgatgcgc
      841 agcaagagcc gcgactccgc acacagaccc agcggcgcgg ccagtcgccg cgccagctcc
      901 catgcggggt tgtggccacg ctcgcgcagg cgagccggat gcaaaggcgt aggcagcagc
      961 aggtcggggc gcggcatgat gcctggctgc agcctggttt cgacggcatg ctgcaagcgc
     1021 gctgcgagcg tgggcgccag atccagcgcg gcgcggaatt tcagcgcgtg cagcagacca
     1081 tcccacggat ggtcgtagtc gagcgcggcg accgtgaact catagggcgg tggctgcttg
     1141 atgcactcgc cgcagagctt ggggccatcc ggcagccgca aggcgcagcg cacgcagcgg
     1201 ttcgtttgcg gcacgaagcg ccgcaaacag ggctcgcaca gccgctcggc ctgccagctg
     1261 tggcagacgg cgcattggct gggccagcgc aaggcgaagg ggcggcggtg cagcagacgg
     1321 ctgaacatga agccctagcc tacactgccc gcccagcatg aatgccgctg cacgagacac
     1381 tagccctgcc acgcgccccg atgccggcgc ggtgcagggc atgctgcgcc gtctggcgcg
     1441 cggcacaggc gcgccttggc tgcacgccga aatcgcaagg cgcatggccg aaaagctgga
     1501 gctgatcaag cgcgagcccg cggtcgtggc ggattggtgg ggcttccttg ggggtggcgc
     1561 agtgcatctg gcacagcgct atccgaaggc gcagcgcgtc gtggtcgagc ccagcgaggc
     1621 cctgcgcgcg cgcagcctcg agcaaggccg ccagccctgg tggtcgccgc ggcgctggcg
     1681 ctcttccgcc accgaggtca ggctcgatgc gatccagccc ggctcggccc agctgctgtg
     1741 ggccaacatg atgctgcact gggtcgacga cgtgcccagc ctgttcgccg aatggcagcg
     1801 cagcctcgcg gtcgacggct tcgtgatgtt ctcgaccttc gggcccgaca cgctgaagga
     1861 gctgcgcgcc ctctatgccc agctgggctg gccgcagccg gcccaggcct ttgtcgacat
     1921 gcacgacatc ggcgaccagc tggtgcacgc gggctttgcc gatcccgtga tggacatgga
     1981 gcagctcacg ctgagctggc ccgagcccca ggccctgctg gccgagctgc gcggcctggg
     2041 ctccaacctc agtgcggcac gcggcaccgg cctgcgcacg ccgcgctggc gcgcccgcct
     2101 ggagcaggaa ttgcagtcgc ttgccggcgc cgacgggcgg ctgtcgctga gcttcgagat
     2161 cgtctacggc catgccttca agcccgcgcc gcggcccagg atcgaaggcg aaaccaaggt
     2221 ctcgctcgag gatatgcgca gcatggtgcg cagcaggcgc ggcggcggcg agggctcgtg
     2281 agggcgaggc cggcgccccg ccgaatgagg aagcggcgcg gacctgagcc gtgccgaaag
     2341 catgacggtg gtcaagggtg aggggcgcgc ggcgctgcgt ataaaatcaa aaagttgtgc
     2401 tcgatcaaag caggcaagtg agtgtgctga cttgcgtgcc ggcgcggagg ctgcgccacc
     2461 gggatgaacc tgaagaagag accaccaaga tgaacacgat gaaagctctg gggctagttc
     2521 tgaggcatgg ggcgcacaag gccggccggc ttgccctgac cctcggcgca gggctggcca
     2581 cgacggcggc cctggccgtc aacgatctgc ccggcggccc cgcggtgctg cagatcgacc
     2641 tgccgcccgc ggcgaccaag atcgccgccg agcagcagtg gctgcactac ttcatgctca
     2701 tcatctgcct ggtgatcttc gcgggcgtgt tcggggtcat gttctattcg atcctgaagc
     2761 accgcaagtc caagggcgcc cagtcggcca atttccacga atccacgacg gtggaaatcg
     2821 tctggaccat cgtccccttc atcatcgtga tcgcgatggc gctgccggcc accaaggccg
     2881 tggtcgcgat gaaggacacg accaactccg acctcaccat caaggccacc ggctaccagt
     2941 ggaaatgggg ctacgactac ctcaagggtg aaggcgaggg catcggcttc atctccacgc
     3001 tcgacgtcaa ccagcgcgtg atgtccaacc tcggccagcc cgagggtgac gactacctgc
     3061 tgaaggtcga caacccgctg gtggtgccgg tcaacaagaa ggtccgcatc atcacgaccg
     3121 ccgccgacgt gatccacgcc ttcgcggtgc cccagttcgg catcaagcag gacgccatcc
     3181 ccggcttcgt gcgcgacacc tggttccgcg ccgagaaggt cggcgacttc tacggccagt
     3241 gccaggaact gtgcggcaag gaacacgcct acatgccgat ccacgtgaag gtggtgtccg
     3301 ccgaggacta caccaagtgg gtcgagggca agaagaagga aatagccgcc gcgtccgagg
     3361 accccaacaa ggtctggacc caggccgagc tgatcgcgcg cggcgagggc gtctacggcg
     3421 ccaactgcgc gacctgccac caggccaacg gcaagggcgg cggtccgatc aagccgctcg
     3481 acggctcgcc cgtcgtgctc gacgccgaca agaccaagca gatcacggtc gtgctccacg
     3541 gccgccaggg cacggcgatg ccgccctgga gccagttgtc cgacaccgac ctggccgccg
     3601 tcatcacctt caccaagaac cactggtcca atgccacggg ccaagccgtg cagcccgccg
     3661 aagtgctggc tgcccgcaag taaacgccag ccacgagaag ctacgaggac ccgaccatga
     3721 gcgcagttct ggaaccccac ggtcacgttg ccggtgacca cgcgcacgac gaccatgcgc
     3781 acggccaccc gcacggctgg cgccgctggg tattcgccac caaccacaag gacatcggga
     3841 cgctgtacct gctgttctcg ttcacgatgc tgctgattgg cggcgtactc gcgctgtgca
     3901 tccgcgccga gctgttcaag cccggcctgc agttcgtgaa ccccgagctg ttcaaccagt
     3961 tcaccaccat gcacggcctg atcatggtgt tcggcgccat catgccggcc ttcgtgggct
     4021 tcgcgaactg gatggtcccg ctgcagatcg gcgcctccga catggccttc gcgcgcatga
     4081 acaacttcag cttctggctg ctgatcccgg ccgcgctgat gctggtgttg tcgttcttca
     4141 tgcctggcgg cgcgccggcc gccggctgga cgctgtacgc gccgctgacg ctgcagatgg
     4201 gtccgtcgat ggacgccggc atcttcgcga tgcacatcat gggcgccagc tccatcatgg
     4261 gctcgatcaa catcatcgtg accatcctca acatgcgcgc ccccggcatg acgctgatga
     4321 ggatgccgct gttcgcgtgg acctggctga tcaccgccta tctgctgatc gccgtgatgc
     4381 cggtgctggc cggcgcgatc acgatgacgc tgaccgaccg ccacttcggc accagcttct
     4441 tcaaccccgc gggcggcggc gacccggtga tgtaccagca catcttctgg ttcttcgggc
     4501 accccgaggt ctacatcatg atcctgccgg ccttcggcat catcagcgcc atcgtcccgg
     4561 ccttcgcgcg caagaagctg ttcggctaca cctcgatggt ctatgccacg gcctcgatcg
     4621 cgatcctgag cttcatcgtc tgggcccacc acatgttcac caccggcatg ccggtgacgg
     4681 gccagctctt cttcatgtac gcgaccatgc tgatcgccgt gcccacgggc gtgaagatct
     4741 tcaactggat cgcgacgatg tggaagggct cgatgacctt cgagacgccg atgctgttcg
     4801 ccgtcggctt catcttcgtg ttcacgatgg gcggcttcac cggcctgatc ctggcgatgg
     4861 cgccgatcga catccagctg caggacacct actacgtcgt ggcccacttc cactacgtgc
     4921 tggtggccgg ctcgctctac gcgatgttcg ccggcttcta ctactggagc ccgaagtggt
     4981 gcggcgtgat gtacaacgaa acgcgcggca agatccattt ctggggttcg ctgatcacct
     5041 tcaacgtcac cttcttcccg atgcacttcc tcgggctggc cggcatgccg cgtcgctatg
     5101 ccgactaccc catgcagttc accgacttca acatggttgc ctcgatcggc gccttcggct
     5161 tcggcctgat gcaggtttat ttcctgctct tcgtggtgct gccggtgatg cgcggccaag
     5221 gcgagaaggc accccagaag ccctgggaag gcgccgaggg ccttgagtgg gaaatcccgt
     5281 cgccggcccc tttccacacc ttcgagactc cgcccaagct cgattccagc gcgacgcgcg
     5341 tgatcggctg agcgccagag atattgcgag gacggcgtga tgacgacccc cgaggaaaag
     5401 aagaagagca atctgcgcct ggcgctgatc ctggcgtcgg tcgcgcttgc cttcttcttc
     5461 ggcttcatcg ccaagatgtc gctgttcgcc catcactgag cgagcgcgcg gaccgcagcc
     5521 atgagcccat ccggaaccga ccgacaagcc gccgcgcgcc gtgccgagaa ccggcgcatg
     5581 gtcggcaagc tcgtggtcgt ggccgccctg atgttcggct tcggctacgc tctggtgccg
     5641 gtctatcgct cgatctgcaa cgccctgggc atcaacgtgc tgtcgctcag cgagctggcc
     5701 gtaccgggcg cgcccggcgc caacggcgtg gccaacacgc aggtcgatcg ctcgcgcacc
     5761 atcacggtcg agttcgatgc caatgcccac gggccctggg acttcaagtc cgagcagcgc
     5821 tcgatgcagg tccatcccgg cgagctcgcc acggtgatgt acgagttcag gaacgtgcag
     5881 gaccacacca tgtcggccca ggcgatcccg agctatgcgc ccaaggaggc gaccgagcat
     5941 ttccacaagc tgcagtgctt ctgcttcacg cagcacacgc tggcgcccgg cgagtccaag
     6001 cgctggccgg tggtcttcgt gatcgacccc aagctgccca agagcgtgac gaccatcacc
     6061 ctgtcctaca ccttcttcga ggtgggcggc gagatgcggg ctgcgcccca gagcactgcc
     6121 ggactgcagc agctcaccaa gccgggggcg gcgtcatgag cgacgctccg cgccccgggg
     6181 ccgacctcaa ggaggcggcg cagcgcaagg gctccttcct gcagacgatg agggccgtag
     6241 cctggtcctt cttcggcgtg cgcaagtcca aggactacga gcaggacgtg agcaagctca
     6301 atccggtgca tgtgatcatc gccggggtca tcggcgcggc aatcttcgtc gtcgtgctgg
     6361 tgctgctggt gcaatgggtg atcggcagcg gcgtggccaa gtagacggc
//
LOCUS       DASUCY010000093         2946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_82105, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000093 DASUCY010000000
VERSION     DASUCY010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82105"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="VFV25_01890"
     CDS             <1..907
                     /locus_tag="VFV25_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY1392093.1"
                     /translation="NPFSGMLGDYAVVMGNTGGDNRVEFGTRLDHAVWYESPNWSGFT
                     INALVSPGQNRATDNSNIPAGGSDCTGGNMPGSGGTPQACNDGSFGTAYSASASYRTS
                     NLLLTAAYEMHKGVNRTSDLAAFDPNDVADEDAGKLGVQYRFPTGTTLMAVVERLRRH
                     VPEYLKYQDERTRSGFWLAVSQDISPAASLHFGWAHAGKTPGDPGQHNTAGGANPDNA
                     ANLYTLAWKYKVDKQLSYYVDWAETLNHAAAHYDLGAGGRGVTTDCHDASNPDASGFD
                     PNGGAPHCWAGGHLQGVSVGMQYKF"
     gene            complement(1025..1582)
                     /locus_tag="VFV25_01895"
     CDS             complement(1025..1582)
                     /locus_tag="VFV25_01895"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY1392094.1"
                     /translation="MSGLGGARLHHVDVALEESALGRGAPAACRVVRAGSLLPVLAES
                     VDRATALHSTRRHRLVVGLLREELQAAEPLAIGIELPDAVSLRAACEAFLQDASDCNL
                     QAMADAAGIGARTLARRFRQELRTSFSEWRLQLRLASVVRAWAEGQTLRTSAAAAGYA
                     SPSAFSVMVRRLVGMTPRRLLASRS"
     gene            complement(1789..>2946)
                     /gene="frc"
                     /locus_tag="VFV25_01900"
     CDS             complement(1789..>2946)
                     /gene="frc"
                     /locus_tag="VFV25_01900"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HEY1392095.1"
                     /translation="RPGSGDVTRNQLRDIPDADALYFTMLNSNKRSLTLDTKTPDGKK
                     VLEDLIKRSDVMVENFGPGALDRMGFTWEYIQKLNPGMILASVKGFSEGHHYEDLKVY
                     ENVAQCAGGAASTTGWWKGENSAPTISAAALGDSNTGMHLAIGILTAYIGRQKTGKGQ
                     KVAVSMQDAVLNLCRVKMRDQQRLERVGYLEEYPQYPHEMEAFADKVTPRGGNAGGGG
                     QPGWILKCKGWETDPNSYIYFTIQGHAWEPICDALGKPEWKSDPAYTTAKARQPHIEE
                     IFATIEDFIKDKTKFEAVDIFRKHDIPCAPVLSMKELLRDESLRKSGSIVEVPHKARG
                     SYFTVGSPIKFSELKPEVTASPLLGEHSDEVLAELGYTPDQIATFHAVGVV"
BASE COUNT          481 a         1001 c          995 g          469 t
ORIGIN      
        1 gaaccccttc tccgggatgc ttggcgacta cgccgtggtg atgggcaaca ccggcggcga
       61 caaccgtgtg gagttcggca cgcggctgga ccacgccgtc tggtatgaat caccgaactg
      121 gagcggcttc accatcaatg cgctggtctc gccgggtcag aaccgcgcga cggacaacag
      181 caacatccct gccggcgggt ccgattgcac cggtggcaac atgccgggca gcggcggcac
      241 accgcaggcc tgcaacgatg gatcgttcgg cacggcttac agcgcgagcg cgagctaccg
      301 cacgagcaat ctcctgctca cggcggccta cgagatgcac aagggcgtga accgcaccag
      361 cgacctggcg gctttcgacc caaatgacgt ggccgacgag gacgcaggca agctgggcgt
      421 ccagtaccgc ttccccacgg gcaccacact gatggccgtg gtggagcgcc tgcggcgcca
      481 cgtgcccgaa tacctgaagt accaggatga acgcacgcgc tcgggcttct ggctcgccgt
      541 cagccaggac atttcaccgg ccgcctcgct gcactttggc tgggcgcatg ccggcaagac
      601 gccgggcgat ccgggccagc acaacaccgc gggcggcgcg aacccggaca acgcggccaa
      661 cctgtacacg ctcgcgtgga agtacaaggt cgacaagcag ctcagctact acgtcgactg
      721 ggccgagacg ctcaatcacg ccgccgccca ttacgacctc ggcgccggcg ggcggggcgt
      781 gactaccgat tgccatgacg cgtcgaaccc ggacgcatcc ggtttcgatc cgaacggtgg
      841 tgcgccgcac tgctgggccg gtgggcacct ccagggcgtg agcgtgggca tgcaatacaa
      901 gttctgaaag cgcagccgca aggagtcgtg atcgtgcgag gtcacgactc cggatgccta
      961 cgaacgacgc acagccctgc ggcggcgctc caggtggaag ccctgcagac cgccggccgc
     1021 aggctcagct gcgagacgcg agtagccgtc gtggcgtcat gccgaccagg cggcgcacca
     1081 tgaccgaaaa ggcgctcgga ctcgcgtagc ccgccgctgc tgcgctggtg cgcagcgttt
     1141 ggccctcggc ccacgcgcgc accacgctgg caagacgcag ctgcaggcgc cactcgctga
     1201 acgaggtgcg caattcctgc ctgaaccgcc gcgccagcgt ccgcgcgccg atgccggcgg
     1261 catcggccat cgcctggagg ttgcagtcgc tggcatcttg aaggaaggcc tcacaggcag
     1321 cgcgcaagct cactgcatca ggcagttcga taccgatcgc cagcggctcg gcagcttgca
     1381 gctcctcgcg cagcaagccg acgacaagac gatggcgtct ggtcgagtga agcgccgtcg
     1441 cgcggtctac ggactctgcc agcacgggca gcaaggagcc tgcgcgcacg acgcggcacg
     1501 cggcaggcgc gccccgccca agggcgctct cttccaacgc gacatccaca tggtggagcc
     1561 gcgcgccgcc caggccgctc acctggtgct ggatctgggc aggaacccac agcgccagcg
     1621 tggcgggcag tatgtggagg ctgcgcgccg tgcggcaata cagcgcgccg ccccgtgcca
     1681 gccacaaggt ccccggcacg aggccttcat cgaatgccgg ataagccgcc ggcccggcat
     1741 gaggcggggc gggcaccgcg cgcgccaaac gccgggcctc gatccgcgtc agaccacgcc
     1801 gaccgcgtgg aaggtggcga tctggtcggg cgtgtagccc agctcggcca gcacctcgtc
     1861 gctgtgctcg cccagcagcg gcgaggcggt gacctcgggc ttgagttccg agaacttgat
     1921 cgggctgccc accgtgaagt agctgccgcg cgccttgtgc ggtacctcga cgattgagcc
     1981 gctcttgcgc agcgactcgt cgcgcagcag ctccttcatc gacagcaccg gggcgcaagg
     2041 gatgtcgtgc ttgcggaaga tgtcgaccgc ctcgaacttg gtcttgtcct tgatgaagtc
     2101 ctcgatggtc gcgaagatct cttcgatgtg cggctggcgg gccttggccg tggtgtaggc
     2161 cgggtcgctc ttccactcgg gcttgccgag cgcgtcgcag atcggctccc aggcatggcc
     2221 ctggatggtg aagtagatgt aggagttggg gtcggtctcc cagcccttgc acttcaggat
     2281 ccagcccggc tggccgccgc cgccggcgtt gccgccgcgc ggcgtcacct tgtcggcgaa
     2341 ggcctccatc tcgtgcgggt actgcgggta ctcctcgagg tagcccactc gctccaggcg
     2401 ctgctggtcg cgcatcttca cgcggcacag gttcagcacc gcatcctgca tcgagaccgc
     2461 caccttctgg cctttgccgg tcttctggcg gccgatgtag gcggtcagga tgccgatggc
     2521 caggtgcatg ccggtgttgg agtcgcccaa ggcggcggcc gagatggtgg gcgccgagtt
     2581 ctcgcccttc caccagccgg tggtggaggc cgcgccgccg gcgcattgcg ccacgttctc
     2641 gtagaccttc aggtcctcgt agtggtggcc ttcgctgaag cccttgaccg acgccaggat
     2701 catgccgggg ttcagcttct ggatgtactc ccaggtgaaa cccatgcggt ccagcgcgcc
     2761 ggggccgaag ttctcgacca tcacgtccga acgcttgata aggtcctcca gcaccttctt
     2821 gccgtcgggc gtcttggtgt ccagcgtcag cgagcgcttg ttgctgttga gcatagtgaa
     2881 gtacagcgcg tcggcgtcgg ggatgtcgcg cagctggttg cgcgtcacgt cgcccgagcc
     2941 ggggcg
//
LOCUS       DASUCY010000094         2923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_676530, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000094 DASUCY010000000
VERSION     DASUCY010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676530"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VFV25_01905"
     CDS             complement(<1..255)
                     /locus_tag="VFV25_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY1392096.1"
                     /translation="MAERLDDTDLLFKALADPSRRKLLDLLHAHDGRTLNELCEHLDM
                     TRQGVTQHLGVLEAANLVATLWRGREKLHFLNPVPLQEIYE"
     gene            complement(431..1441)
                     /locus_tag="VFV25_01910"
     CDS             complement(431..1441)
                     /locus_tag="VFV25_01910"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEY1392097.1"
                     /translation="MLEEALSAARVVADQEREAGPGLAALGQALARQAPGLALTVARG
                     SSDHAASYFAYLAMQRIGVPVVSLPMSLVTLHRAPLAVQGQLAVALSQSGRSPDLVDT
                     MVTLGGAGARTVALVNQPDSPLAAACEWTVPLCAGAEKSVAATKSYIASLSAVARLVA
                     HWRDDAALLQALQALPEHLLAATREDWSPAVEALAKAERIMVVGRGLGFAIALEAALK
                     FKETCAIQAEAFSSAEIKHGPMALIHQGYPLLVFAPRGPEQSGLIALAAEMRERGAKV
                     LLAAPADVPTRQLTLATAADEVLDPLLAIQSFYLMAATLSEARGLNPDAPLHLSKVTL
                     TV"
     gene            complement(1434..2555)
                     /gene="nagA"
                     /locus_tag="VFV25_01915"
     CDS             complement(1434..2555)
                     /gene="nagA"
                     /locus_tag="VFV25_01915"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEY1392098.1"
                     /translation="MTNTASLQGRILTPAGWVEGRLSSSPAGRIAAIEAARLSPEDST
                     RTLLPGFIDLHVHGGGGTDVMEGGSAAERVAQLHARHGTTSLLATTMTAPMGEIETAL
                     APLGHYCRERSAGAARILGVHLEGPYINPGKLGAQPHHTCVAVLEEVQRLHALAPLRV
                     ITLAPEITGHLELIGRLNDLGMRVQIGHSLASYEEGLSALQHGAAGFTHLFNAMSGMH
                     HREPGAVGAALAHAEYAELIPDLLHVHPGAMHAALRAIPRLYCVTDSTSAAGMPDGEY
                     HLGSQHVTKCLGGVRLADGTLAGSTLTMEEALRNLVSIGLSLEDASNRLSRYPADYLG
                     LNERGRLAPGAWADVVELDAGLRLKNVYVEGERIALVDA"
     gene            complement(2727..>2923)
                     /locus_tag="VFV25_01920"
     CDS             complement(2727..>2923)
                     /locus_tag="VFV25_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY1392099.1"
                     /translation="PETGANADTANSGATLVYLSPGATVLLTDKWQLYGFVQLPIYQR
                     VNGLQLETKTSFSLGTRVAF"
BASE COUNT          453 a         1004 c         1002 g          464 t
ORIGIN      
        1 ctcgtagatt tcctgcagcg gcaccgggtt aaggaaatgc aatttctcgc ggccgcgcca
       61 caaggtggct accaggttcg ccgcctccag cacgccaagg tgctgcgtca cgccctgccg
      121 cgtcatgtcc aggtgctcgc acagctcgtt cagcgtgcgg ccatcgtgcg cgtgcagcaa
      181 gtccagtagc ttgcgccggc tcggatcggc gagggctttg aacagcaagt cggtgtcatc
      241 gaggcgctcg gccatgaacg gctacatgca agtaatctgt tgcatgaata ataggcaagc
      301 aatcccttgc atgtcaagcg ctggtttgac gcgtcaccaa cgcttggcac tccgagcttg
      361 cgctgcttgc cgccttgggg cggccttgtt gcgtcgaact cggccgtgcg gttcagggcc
      421 tctgctggcg ctaaaccgtc agcgtcacct tcgacagatg caagggcgcg tccgggttga
      481 gcccgcgcgc ctcagacagc gtggcggcca tcaggtagaa gctctggatc gccagcagcg
      541 ggtcgagcac ctcgtccgcg gcggtggcca gcgtcagctg gcgtgtcggc acatcggccg
      601 gcgcggctag cagcactttc gcgccgcgct cgcgcatctc ggcggccagc gcgatcagcc
      661 cggactgctc cggtccgcgt ggcgcgaaca ccagcagcgg gtagccttgg tggatcagcg
      721 ccatcggccc atgcttgatc tcggcgctgg agaaggcctc ggcctggatc gcgcaggtct
      781 ccttgaactt cagcgctgcc tccagcgcga tcgcgaagcc cagcccgcgg cctactacca
      841 tgatgcgctc ggccttggcc agcgcttcga ccgccggcga ccagtcttcg cgcgtggcgg
      901 cgagcagatg ctcgggcagc gcctgcaggg cctgcagcag cgctgcatcg tcgcgccaat
      961 gcgccaccag gcgcgcgacg gccgacaacg aggcgatata gctcttggtc gccgccacgc
     1021 tcttctcggc gcccgcgcac agcggcacgg tccactcgca ggcggccgcc aagggtgaat
     1081 cgggctggtt caccagcgcc accgtgcggg cgccggcgcc gcccagcgtg accatcgtgt
     1141 ccaccaggtc cggactgcgg cccgattggg acagcgccac cgccagctgg ccttgcacgg
     1201 ccagcggcgc acggtgcagc gtcaccagcg acatcggcag cgaaaccacc ggcacgccga
     1261 tacgctgcat cgccagatag gcgaaatagc tggccgcatg gtcggagctg ccgcgcgcca
     1321 ccgtcagcgc caggcccggg gcctggcgcg ccagcgcctg accgagcgcc gccaggcccg
     1381 gccccgcctc gcgctcctga tcggcgacga cccgcgcggc ggatagggct tcctcaagca
     1441 tcaacgaggg caatgcgttc tccttcgaca tacacgttct tgagccgtag cccggcatcg
     1501 agctcgacca cgtcggccca ggcgccgggc gccagccggc cgcgctcgtt caagcccagg
     1561 tagtcggccg ggtagcgcga aagccgattc gaggcgtctt ccaggctcag tccgatggag
     1621 accaggttgc gcagcgcctc ttccatggtc agcgtggagc cggccagcgt gccgtcggcc
     1681 aggcgcaccc cgcccaggca tttggtgacg tgctgcgagc ccaggtggta ttcgccatcg
     1741 ggcatgccgg cggcggacgt ggaatcggtg acgcaataca ggcgtgggat cgcgcgcagc
     1801 gccgcgtgca tcgcgcccgg atgcacgtgc agcagatcgg ggatcagctc ggcgtattcg
     1861 gcatgggcca gtgccgcgcc gaccgcgccg ggctcgcggt ggtgcatccc gctcatcgcg
     1921 ttgaacagat gggtgaagcc ggccgcgccg tgctgcagcg cgctcaggcc ttcctcgtag
     1981 ctggccaggc tgtggccgat ctgcacccgc atgcccagat cgttcaagcg gccgatcagc
     2041 tccagatggc cggtgatctc gggggccaag gtgatgacgc gcagcggggc cagggcatgc
     2101 aggcgctgca cctcttccag caccgcgacg caggtgtgat gcggctgggc gcccagcttg
     2161 ccagggttga tgtaggggcc ttccagatgc acgcccagga tgcgcgcggc gccggcgctg
     2221 cgctcgcggc agtagtggcc gagcggcgcc agcgcggttt cgatctcgcc catcggcgcg
     2281 gtcatcgtgg tggcgagcag cgaggtggtg ccgtgccggg catgcagctg ggccacgcgt
     2341 tcggcggccg agccgccttc catcacatcg gtgccgccgc cgccgtgcac atgcagatcg
     2401 atgaagccgg gcagcagcgt gcgcgtgctg tcttcaggag aaagccgcgc cgcctcgatg
     2461 gcggcaatgc ggcccgccgg actcgagctc aggcggcctt ccacccaacc ggcgggagtc
     2521 aggatgcggc cttgcaggga agcggtattc gtcatcgcgg aaggggggcg agggccccga
     2581 agatcagccc tcagtctagc cccgtacccg cgcggaaacc gcgccaactg gtatgccgtg
     2641 aaggcgagcg gttcaggccg cccgctccct ctgaaacgcg gctttttcgc cgcgaatccg
     2701 ctcgcctacc agtgccgatt tttcattcag aaggcgacgc gcgtgcccag cgagaagctg
     2761 gtcttggtct ccagctgcag gccgttgacg cgctggtaga tcggcagctg aacgaagccg
     2821 tagagctgcc atttgtcggt cagcaacacc gtcgcgcccg gactcagata gaccaaggtc
     2881 gcgccggagt tggcggtgtc ggcattggcg cccgtttcgg gcc
//
LOCUS       DASUCY010000095         2162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1511988, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000095 DASUCY010000000
VERSION     DASUCY010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1511988"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="VFV25_01925"
     CDS             <1..756
                     /locus_tag="VFV25_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY1392100.1"
                     /translation="AVISGFGVGAIVGLTGVGGGSLMTPLLISAFRLNPAVAIGTDLW
                     FAALTKTTGSIAHHRAGNVDYRVMSLLLVGSLPASLATIGLMHATGLTKAWASALTVT
                     LGIALLLTAVTVAYRQAWQALGLRLERVITPERRPALTVLAGLILGVLVSLSSIGAGA
                     IGATLILLLYPRMEARRLVGTDIAHAVPLTLIAGIGHASLGHVDWGLLAALLLGSIPG
                     IWIGAQLTKKMPDRVVRALLCVSLVSAGLKVIS"
     gene            792..>2162
                     /locus_tag="VFV25_01930"
     CDS             792..>2162
                     /locus_tag="VFV25_01930"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEY1392101.1"
                     /translation="MYQYTEFDRYFVRQRAAQFRDQLERNIAGTLSDDDFRPLRLQNG
                     WYIQRHAPMTRIAVPYGELSSRQLRQLAYVAREFDRGYAHFTTRQNCQFNWIPLPRAA
                     DTMDALAEVDMHGIQTSGNCIRNITSDALAGVAPDEIVDPRPYCEILRQWSTLHPEFA
                     FLPRKFKIAVSGAKEDRAAIAWHDIGLQLLKNEAGEVGFKVLAGGGMGRTPIPGTVVH
                     EFLPWQQILVFIEAIVRVYNRYGRRDNMYKARIKILVKAEGERFIKQVHDEFQAILER
                     DVDGLAHIVPQREFDRVAASFVLPEGVAPRASKGDGAPADAPLAYRKWLERNVHGHKI
                     PGYRAVTLSLKRAGQAPGDATDAQMDLAADLAERYSSSELRVTHDQNLLLPWVRESDL
                     YALWQEARAASFATPNIGLLTDMIACPGGDFCALANARSIPIAAALTERFDDLDELYD
                     IGDIDLH"
BASE COUNT          331 a          798 c          691 g          342 t
ORIGIN      
        1 gcggtcatca gcggatttgg cgtcggcgcc atcgtcggcc tcaccggcgt gggcggcggc
       61 tcgctgatga cgcccttgct gatctcggcg ttccggctga acccggcggt cgcgatcggc
      121 accgacctgt ggttcgccgc cctgaccaag acaacgggct cgatcgccca ccatcgcgcg
      181 ggcaatgtcg actaccgcgt gatgagcctg ctgctcgtcg gcagcctccc cgcctcgttg
      241 gccacgatag gcctgatgca cgccaccggc ctcaccaagg cctgggccag cgcgctcacg
      301 gtgaccctgg gcatcgcgct cctgctgacc gctgtcaccg tcgcctatcg ccaagcctgg
      361 caggccctgg gcttgcgcct ggagcgcgtg atcacgcccg agcgccggcc ggcgctgacc
      421 gtgctcgccg gcctgatcct gggcgtgctg gtgtcgctga gctcgatcgg cgcgggcgcg
      481 atcggcgcca cgctgatcct gctgctgtac ccgcgcatgg aagcgcgccg cctggtcggc
      541 accgacatcg cccatgccgt gccgttgacg ctgatcgccg gcatcggcca tgcctcgctc
      601 ggccatgtgg actggggcct gctcgctgcg ctgctgctgg gttccatccc cggcatctgg
      661 atcggcgccc agctcaccaa gaagatgccg gaccgcgtgg tgcgggcgct gctgtgcgtc
      721 tcgctcgtca gcgccgggct caaggtcatt tcctgattcc ttcgcccggg ccttgcgccg
      781 gggccactgc catgtaccag tacaccgagt tcgatcgata tttcgtccgc cagcgcgcgg
      841 cgcagtttcg cgaccagctg gagcgcaaca ttgctggcac gctgagcgat gacgatttcc
      901 ggccgctgcg gctgcagaac ggctggtaca tccagcgcca cgcgccgatg actcgcatcg
      961 ccgtccccta cggcgaactc agcagccgcc agctacgcca gctggcctat gtcgcacgcg
     1021 agttcgaccg cggctatgcc catttcacga cgcgccagaa ctgccagttc aactggattc
     1081 cgctcccacg cgcggccgac acgatggatg cgctggccga ggtcgacatg cacggcatcc
     1141 agaccagcgg caactgcatc cgcaacatca cgagcgacgc gctggccggc gtggcgcccg
     1201 acgaaatcgt cgatccgcgc ccctattgcg agatcctgcg ccagtggtcg acgctgcacc
     1261 ccgagttcgc cttcctgccg cgcaagttca agatcgcggt cagcggcgcc aaggaagacc
     1321 gcgccgccat cgcctggcat gacatcggcc tgcagctgct gaagaacgag gccggcgaag
     1381 tcggcttcaa ggtgctggcc ggcggcggca tgggccgcac gcccatcccc ggcacggtgg
     1441 tccacgagtt cctgccctgg cagcagatcc tcgtcttcat cgaggccatc gtgcgcgtct
     1501 acaaccgcta cggccggcgc gacaacatgt acaaggcgcg catcaagatc ctcgtcaagg
     1561 ccgagggaga gcgcttcatc aagcaggtgc acgacgagtt ccaggccatc ctggagcgcg
     1621 acgtcgacgg cttggcgcac atcgtcccgc agcgcgagtt cgaccgcgtg gccgcaagct
     1681 tcgtgctgcc cgagggcgtc gcaccgcgcg cctcgaaggg cgacggcgcc cctgccgatg
     1741 cgccgctggc ctaccgcaag tggctggagc gcaatgtgca cggccacaag atccccggct
     1801 accgcgccgt gacgctgtcg ctcaagcgcg ccggacaggc gcccggcgac gccaccgacg
     1861 cgcagatgga cctggccgcc gatctggccg agcgctactc cagcagcgag ctgcgcgtca
     1921 cgcacgacca gaacctgctg ctgccctggg tgcgcgagtc ggacctgtac gcactgtggc
     1981 aggaggccag ggccgccagc ttcgcgacac ccaacatcgg cctcttgacc gacatgatcg
     2041 cctgccccgg cggcgacttc tgcgcgctgg ccaatgcccg ctcgatcccg atcgccgcgg
     2101 cgctgaccga gcgcttcgac gacctcgacg agctctacga catcggcgac atcgacctgc
     2161 ac
//
LOCUS       DASUCY010000096         2899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_901541, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000096 DASUCY010000000
VERSION     DASUCY010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901541"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VFV25_01935"
     CDS             <1..309
                     /locus_tag="VFV25_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEY1392102.1"
                     /translation="LVDAKDVISSAYKVVVARGNVYLMGRVTEREANRGADLARSIGG
                     VQKVVKVFEILSEQELAALGRQTRAAPVSDAASQPTSTPAPQPAEQQPEAAQPNPVRN
                     "
     gene            complement(384..1280)
                     /locus_tag="VFV25_01940"
     CDS             complement(384..1280)
                     /locus_tag="VFV25_01940"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY1392103.1"
                     /translation="MSSSKQYEAVAPHTVAFLGLGVMGHPMAGHLARAGHRVTVYNRT
                     ADKAAGWAREYGGKTAATPREVAAGTELVFACVGNDDDLRSITLGEQGAFAGMKPGAV
                     FVDHTTASAEVARELSAAASERGLRFIDAPVSGGNLGAINGTLTVMCGGDALAFAKAQ
                     PVAMAYAKAVTLLGGSGAGQLAKMVNQIAIAGLVQGLAEAIAFGRKAGLDMRSVLGVI
                     GKGAAQSWQMDNRGPTMIEDQFDFGFAVDWMRKDLDLCLNEARRNGARLPVTALVDQF
                     YADVQALGGGRWDTSSLIKRLG"
     gene            complement(1344..2480)
                     /locus_tag="VFV25_01945"
     CDS             complement(1344..2480)
                     /locus_tag="VFV25_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEY1392104.1"
                     /translation="MERDQASKFINDLLRLMVSRNGSDLFLTSDFPPAVKVDGKVTKV
                     SPQPLTGQHTIALARSIMNDKQAAEFERTKECNFAIAPQGVGRFRVNAFVQQGNVGMV
                     LRTIPQTLPTIDGLGLPHVLKEVAMTKRGLVVFVGATGSGKSTSLAAMVDYRNENSYG
                     HIITIEDPVEFVHPHKNCIVTQREVGIDTEDWFAALKNTLRQAPDVILMGEIRDRETM
                     EHAVAFAETGHLCLATLHANSANQALDRIINFFPEERRAQLLMDLSLNLKALISQRLL
                     PRQEGKGRVAAVEIMLNTPLMADLIFKGEVGELKELMKRSRELGMQTFDQSLFDLYEG
                     QSITYEDALRNADSVNDLRLQIKLNSQRARNADLSAGTEHLTIV"
     gene            complement(2492..>2899)
                     /locus_tag="VFV25_01950"
     CDS             complement(2492..>2899)
                     /locus_tag="VFV25_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY1392105.1"
                     /translation="SAQDLQRMSDYLEFVTVAENQQVIGQDEQGDYMLIVLEGTLAVD
                     RLQPWGARVRLAEAHAGDMLGEMSLLDAGARFSVCTTLTPCCLAVVVAQRLDEMIMFE
                     PRLGLALLASLSRRLSLRLRHVSARLSALLSRG"
BASE COUNT          479 a          972 c          951 g          497 t
ORIGIN      
        1 ctggtggacg ccaaggatgt catctccagc gcctacaagg tggtggtcgc gcgcggcaac
       61 gtctacttga tgggccgcgt caccgagcgc gaggccaatc gcggcgccga tctggcgcgc
      121 agcatcggcg gcgtgcagaa ggtcgtcaag gtattcgaga tcctcagcga gcaggagctc
      181 gccgcgctgg ggcgccagac gcgcgccgcc ccggtgtctg atgccgcctc ccagcccact
      241 agtacgccgg cgccccagcc tgcggaacag cagcccgaag cggcccagcc caacccggtc
      301 agaaactgag agttttctga ccagttaccc aaggagttct gcgcaagatc actacgaaaa
      361 acgtagtgat cttgcctgaa cgctcagccc agccgcttga tcaggctgga cgtgtcccaa
      421 cgcccgccac ccagcgcctg cacatcggca tagaactggt ccaccagggc cgtgaccggc
      481 aggcgcgcgc cgttgcgcct ggcctcgttg aggcacaggt cgaggtcctt gcgcatccag
      541 tccaccgcga agccgaagtc gaactggtcc tcgatcatcg tgggcccgcg gttgtccatc
      601 tgccagctct gtgccgcacc cttgccgatc acgcccagca cggagcgcat gtccagcccg
      661 gctttgcggc cgaaggcgat cgcctcggcc aggccctgca ccaggccggc gatagcgatc
      721 tggttgacca tcttggcgag ctggccggcg ccgctgccgc ccagcagggt cacggccttg
      781 gcataggcca tcgcgaccgg ctgcgccttt gcgaaagcca gtgcatcacc gccgcacatg
      841 acggtcagcg tgccgttgat cgcgcccagg ttgccgcccg agaccggggc atcgatgaat
      901 cgcaggccgc gttcgctggc cgctgcgctg agctcgcgcg cgacctcggc cgaagcggtc
      961 gtgtggtcga cgaagacggc gcccggcttc atgccggcga aggccccttg ctcgcccagc
     1021 gtgatcgaac gcagatcatc atcgttgccg acgcaggcga agaccagttc agtacctgca
     1081 gctacctccc gcggcgtggc tgccgtcttg ccgccgtact cgcgcgccca gccggccgcc
     1141 ttgtctgccg tgcggttgta gaccgtgaca cgatgcccgg cgcgcgccag gtggcccgcc
     1201 ataggatggc ccatcacgcc caggccgagg aaggcgacgg tatgcggggc gacggcttcg
     1261 tattgcttgc tgctgctcat gggctggttc tccaaacgaa aacggcctcg aaccgaggcc
     1321 gttcttcagc gccgaatgat cgcctaaacg atcgtcaggt gctcggtgcc ggcggacagg
     1381 tccgcgttgc gcgcgcgctg gctgttgagc ttgatctgca ggcgcaggtc gttcaccgag
     1441 tcggcattgc gcagcgcatc ctcgtaagtg atggactggc cttcgtagag atcgaacagg
     1501 ctctggtcga aggtctgcat gcccagctcg cggctgcgct tcatcagctc cttgagctcg
     1561 ccgacctcgc ccttgaagat caggtccgcc atcagcggcg tgttgagcat gatctccacc
     1621 gccgccacgc ggcccttgcc ctcctggcgc ggcagcaggc gctgcgagat cagcgccttg
     1681 aggttgagcg agaggtccat cagcagctgg gcccggcgct cctcggggaa gaagttgatg
     1741 atgcggtcca gcgcctggtt ggcgctgttt gcgtgcagcg tggctaggca caggtgcccg
     1801 gtctcggcga aggcgaccgc atgctccatc gtctcgcggt cgcggatctc gcccatcagg
     1861 atcacgtcgg gcgcctggcg cagcgtgttc ttcagcgcgg cgaaccagtc ctcggtgtcg
     1921 ataccgacct cgcgctgcgt gacgatgcag ttcttgtgcg ggtgcacgaa ctccaccggg
     1981 tcctcgatcg tgatgatgtg gccgtaggag ttctcgttgc gatagtccac catcgccgcc
     2041 agcgaagtcg acttgcccga gccggtggcg ccgacgaaga cgaccaggcc gcgcttggtc
     2101 atcgccactt ccttcagcac atggggcagc cccagcccgt cgatggtcgg cagcgtctgc
     2161 gggatcgtgc gcagcaccat gccgacattg ccctgctgca cgaaggcatt gacgcggaag
     2221 cgccccacgc cctgcggcgc gatggcgaaa ttgcattcct tggtgcgctc gaactcggct
     2281 gcctgcttgt cgttcatgat cgaacgcgcc agcgcgatcg tgtgctggcc ggtgagcggc
     2341 tggggcgaca ccttggtgac cttgccgtcg accttgacgg cgggcgggaa gtcggaagtc
     2401 aggaagagat ccgagccgtt gcgcgacacc atcaggcgca gcagatcgtt gatgaacttg
     2461 gatgcctggt cgcgttccat gatgggctac ctcagccgcg tgacagcaag gcgctgaggc
     2521 gcgcactgac atggcgcagc cgcagcgaca agcgccgcga cagggaggcc agcagggcca
     2581 gtccgagacg cggctcgaac atgatcatct catccaatcg ttgcgcaacc accacagcaa
     2641 ggcaacaagg tgtgagtgtg gtgcagaccg agaagcgcgc gccggcgtcg agcagtgaca
     2701 tctcgccgag catgtcgccg gcatgggcct cggccagcct gacgcgcgcg ccccagggct
     2761 gcaggcggtc gacggccagc gtgccttcga gcacgatcag catgtagtcg ccctgctcgt
     2821 cctggccgat gacctgctgg ttctcggcca ccgtgacgaa ctcgagatag tcgctcatgc
     2881 gctgcaggtc ctgcgcgct
//
LOCUS       DASUCY010000097         3865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_403571, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000097 DASUCY010000000
VERSION     DASUCY010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403571"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VFV25_01955"
     CDS             complement(<1..446)
                     /locus_tag="VFV25_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392106.1"
                     /translation="MNTINDSLPPSALPVEEESASPVPTEDQAAAPVRKRRTRKSEAQ
                     APEAAAEATPAGDAGTEAPAEPAAAPKRTRKRKAVAAAEEAAPIAGATEEAPPVAGAD
                     EPASVPVAEPVAAEAGPSLAPVAADENAAEVGPAADATDAGPAAEG"
     gene            complement(458..1249)
                     /gene="scpB"
                     /locus_tag="VFV25_01960"
     CDS             complement(458..1249)
                     /gene="scpB"
                     /locus_tag="VFV25_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEY1392107.1"
                     /translation="MNTQDAKRVLETALICAQQPMPLRDMRTLFDDQINLDTLRGLLD
                     ELTRDWEDRGVELVALASGWRFQSRPEIRHYLDRLHPEKPPKYSRATMETLSIIAYRQ
                     PVTRGDIEDIRGVTVSSQIVKQLEDRGWIEAIGYREAPGRPALYATTRQFLDDLGLAS
                     LEQLPMIDGNATPAAAVLGEALLPEQASLLDGAGEQVTEPMAAPIPEEAPAAEADQEL
                     PNHATRSIDGEADAQIEPLAAEAAAEHEAAEPREMTQEPSSHTEA"
     gene            complement(1502..2515)
                     /locus_tag="VFV25_01965"
     CDS             complement(1502..2515)
                     /locus_tag="VFV25_01965"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEY1392108.1"
                     /translation="MRATSSSEPDRLQEIDEEAAEPQAAEVERRCLRVDEAAHGLRLD
                     KLLVTMAGEFSRSHLQNLIAQGHVRLAGQVQTTASRKVQLGQEVEVDLLPTEQSKAFC
                     AEAVPLAIVYEDEHLMVVDKPAGLVVHPAAGNWSGTLLNGLLHHHAGAANLPRAGIVH
                     RLDKDTSGLMVVGKTLAAVTALVREIGAREVRREYLALAHGAVADEAFSIDAPIGRDP
                     VSRVRMAVLASGKPARTDVQRIAVGEALGQSGVPELASALRCRLHTGRTHQIRVHLAH
                     QGHPLVGDTLYGGRRLLGLQRQALHAARLSFTHPASGRPMDFEAALPPDLAAAWQRLL
                     GSR"
     gene            2576..3394
                     /locus_tag="VFV25_01970"
     CDS             2576..3394
                     /locus_tag="VFV25_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEY1392109.1"
                     /translation="MSQFRLEFGGQRLRTIAATLATAALLGACGTTKDEFAGQSNEKL
                     YADAREEVANGNYEKGAKIYEKLKGRAAGTLMAQQAELELAWTYYKSQEKAQALSTIE
                     SFIKLHPSSPALDYALYLQGLINFNDDLGLFGRIAGQDLSERDQQASRDAYQSYKQLV
                     DQFPESKYAPDARLRMNYIVNTLAAYEVHVARYYYRRGAYVAAANRAQQAVQEFSSAP
                     AVEEALYIMMLSYDRLDLPKLHDDAQRVLTKNFPTSRFLSEGFQRPNKPWWQLW"
     gene            complement(3400..>3865)
                     /locus_tag="VFV25_01975"
     CDS             complement(3400..>3865)
                     /locus_tag="VFV25_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HEY1392110.1"
                     /translation="PGEPIQVLVQGRSPKRQLLQQFLASPRSVLVGSQSFWEGIDVPG
                     DALQCVLIDKLPFPPPNDPLVEARVRRLEAQGRNAFGDYFVAEAAVALKQGAGRLIRT
                     ETDRGLLVVCDPRMARMNYGKRLRAALPAMGRLDEEAEVLDWLAELSAAHRV"
BASE COUNT          573 a         1327 c         1324 g          641 t
ORIGIN      
        1 tcgccttcag ccgcggggcc tgcatcggta gcgtcggcag ccgggccgac ctcggctgcg
       61 ttctcatccg ccgcgaccgg tgccagcgac ggacccgctt cggcagccac tggctcggcg
      121 accggaacgc tggctggctc atcggcgccg gcgaccgggg gcgcctcttc agtggcgccg
      181 gcgatcggcg ccgcttcttc ggcagcagcc acggctttgc gcttgcgcgt ccgcttgggc
      241 gctgcggccg gctcggctgg cgcctcggtg cctgcatcgc cagccggcgt cgcctcggca
      301 gccgcttcgg gcgcctgggc ttcggacttg cgagtgcggc gcttgcggac cggcgcagcg
      361 gcctggtctt cggtggggac cgggctggcc gattcttctt ccacgggcaa ggcggagggc
      421 ggcaggctgt cgttgatggt gttcattcga ttaggcgtca agcctcggtg tgcgaggacg
      481 gctcttgggt catttcacga ggctcggccg cctcgtgctc ggccgcggct tcggcggcca
      541 ggggttcgat ctgcgcatcg gcttcgccgt cgatgctgcg cgtcgcgtgg tttggtagct
      601 cctggtccgc ttcggcggcg ggcgcctctt cgggtatcgg tgcggccatc ggctcggtga
      661 cttgctcgcc ggcgccatcg agcaaggagg cctgctcggg cagcaaggcc tcgcccagca
      721 cagccgcggc gggcgttgca ttgccatcga tcatcggcag ctgctccagc gatgcgaggc
      781 cgaggtcgtc gaggaactgg cgcgtggtcg cgtacagcgc gggccggccg ggcgcctcgc
      841 ggtagccgat tgcctcgatc cagccgcggt cttcgagctg cttgacgatc tggctgctga
      901 cggtgacgcc gcggatgtcc tcgatgtcgc cgcgcgtcac cggctggcgg taggcgatga
      961 tggacagcgt ctccatcgtc gcacgcgagt atttcggcgg cttctccgga tgcaggcggt
     1021 cgagatagtg gcgtatctcg gggcggctct ggaagcgcca gccgctggcc agcgcgacca
     1081 gctcgacgcc gcggtcttcc cagtcgcgcg tcagctcgtc gagcaggccg cgcagggtgt
     1141 ccagattgat ctggtcgtcg aagagcgtgc gcatgtcgcg cagcggcatg ggctgctgtg
     1201 cgcaaatgag cgccgtctct aggacacgct tggcatcctg tgtgttcatg gaatccgttt
     1261 cactccgggg accgaaacaa ggggggccac ggtggctgaa gcctggcggg ctgcgcgtct
     1321 ttgcttgctg cttccggctg tcgccgaagc atcgtcgtgt cgcgctgggc cgggtaagcc
     1381 tgggggcatc gaggcctgtg catggcaggc actctctttg gggccaccag ggtgggagcc
     1441 ttgccggcct aaacggcttg ggcggctgcg ccagggagct ttgcaccgca ttgtagccgg
     1501 ctcaacggga acccagaagc cgctgccagg cggcggcaag atccggcggc aaggccgcct
     1561 cgaaatccat gggcctgccg ctggccggat gcgtgaagct caggcgcgcc gcgtgcaggg
     1621 cctggcgctg caggcccagc aagcggcgcc cgccataaag cgtgtcgccc accagcggat
     1681 gcccctggtg agccaggtgc acgcggatct ggtgggtgcg gccggtgtgc aggcggcagc
     1741 gcaaagcact ggcaagctcc ggcaccccac tctgcccaag cgcctcgccc accgcgatgc
     1801 gctggacgtc ggtgcgtgca ggcttgccgc ttgccagcac cgccatgcgc acgcgcgaga
     1861 ccggatcgcg cccgatcggc gcgtcgatgc tgaaggcctc gtcagcgacg gcgccatggg
     1921 ccagcgcgag atattcgcgg cgcacctcgc gcgcgccgat ctcgcgcacc agcgccgtca
     1981 ccgcagccag cgtcttgccg accaccatca agccgctcgt gtccttgtcc agcctgtgca
     2041 cgatgccggc gcgcggcagg ttcgccgcgc cggcgtggtg atgaagcaag ccattgagca
     2101 gagtgcccga ccaattgccg gccgccggat gcacgaccag ccccgcaggc ttgtcgacca
     2161 ccatcagatg ctcgtcctcg tacacgatgg ccagcggcac tgcttcggcg cagaaggcct
     2221 tgctctgctc ggtgggcagc agatcgacct cgacctcctg ccccagctgc accttgcgcg
     2281 aagccgtggt ctgcacctgc cccgccagcc tcacatgccc ctgcgcaatc aggttctgca
     2341 gatggctgcg agagaactcg cccgccatcg tgaccagcag cttgtccagc ctcaggccat
     2401 gtgcagcctc gtcgacacgc aggcagcgcc gctcgacctc ggcggcctgg ggctcggcgg
     2461 cttcttcgtc gatctcctgg agccgatccg gctcggagct gcttgtggcc ctcatatact
     2521 gtcgtttggt tcctaggcag cagcggtggc aagcccccgc taacgagtgc gagtgatgag
     2581 tcagtttcgt ttggagttcg gagggcagcg cctgcgcacg atcgcggcaa ccctcgccac
     2641 ggctgcgctg ctgggcgcct gcggcaccac caaggacgag ttcgcgggcc agagcaatga
     2701 aaaattgtac gcggacgccc gcgaggaagt ggccaacggc aactatgaga agggcgccaa
     2761 gatctacgag aagctcaagg gccgcgccgc cggtacgctg atggcgcagc aggccgagct
     2821 ggagctggcc tggacctact acaagagcca ggagaaggct caggccctgt ccaccatcga
     2881 gagcttcatc aagctgcacc cctccagccc ggcgctggac tatgcgctgt atctgcaggg
     2941 cctgatcaac ttcaacgacg acctgggcct gttcggccgc atcgccgggc aggacctgtc
     3001 cgagcgcgac cagcaggctt cgcgcgacgc ctaccagtcc tacaagcagc tggtcgatca
     3061 gttccccgaa tccaagtacg cccccgatgc gcgcctgcgg atgaactaca tcgtcaacac
     3121 gctggcggcc tacgaggtgc acgtggcgcg ctactactac cgccgcggcg cctatgttgc
     3181 ggcggccaac cgcgcgcagc aggcagtgca ggaattcagc agcgcgccgg cggtcgagga
     3241 agcgctctac atcatgatgc tgagctacga ccgcctggac ctgcccaagc tgcacgacga
     3301 tgcacagcgc gtgctgacca agaacttccc caccagccgc ttcctcagcg agggcttcca
     3361 gcgccccaac aagccttggt ggcagctgtg gtgactggct cagaccctgt gggctgctga
     3421 cagctcggcg agccagtcca gcacctcggc ctcctcgtcg agccggccca tggccggcag
     3481 cgcggcgcgc agccgtttgc cgtagttcat gcgcgccatg cgcggatcgc acaccaccag
     3541 caggccgcga tcggtctcgg tgcgtatcag gcgccccgcc ccctgcttga gcgcgacggc
     3601 ggcctcggcg acgaaatagt cgccgaaggc attgcggccc tgggcctcca gccttctcac
     3661 gcgcgcctcg accagcgggt cgttgggcgg cggaaagggc agcttgtcga tcagcacgca
     3721 ttgcaaggcg tcgccgggca catctatgcc ctcccagaag ctctgcgagc cgaccagcac
     3781 gctgcgcggc gaggccagga attgctgcag cagctggcgc ttgggcgagc ggccctgcac
     3841 cagcacctgg atgggctcgc ccggc
//
LOCUS       DASUCY010000098         3279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1576419, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000098 DASUCY010000000
VERSION     DASUCY010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1576419"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="VFV25_01980"
     CDS             complement(<1..756)
                     /locus_tag="VFV25_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HEY1392111.1"
                     /translation="MEVALVLAVIAAIFVIKSVTVVPQQHAVVVERLGRYHATLTPGL
                     NFLVPFIDRQAYRHSLKEIPLDVPSQVCITKDNTQLQVDGILYFQVTDPMRASYGSSN
                     YIVAITQLAQTTLRSVVGKMELDKTFEERDAINAQVVTALDEAALNWGVKVLRYEIKD
                     LTPPAEILRAMQAQITAEREKRALIAASEGRRQEQINIATGEREAFIARSEGAKQAEI
                     NKAQGEAAAITAVADATADAIRKIAASIQQPGGE"
     gene            complement(800..1222)
                     /locus_tag="VFV25_01985"
     CDS             complement(800..1222)
                     /locus_tag="VFV25_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEY1392112.1"
                     /translation="MTDAMLWWVVAGAAVALELATGTFYLLMLALGLSAGAIAAHLGL
                     GTEAELVAAALVGGGAVAACYLVRSRLPAKAPAAKNRDMNLDIGERVQVQQWNSDGTA
                     RIHYRGAAWSARYVGDGEPAPGEHFIQAIEGNQLLLGR"
     gene            1361..>3279
                     /locus_tag="VFV25_01990"
     CDS             1361..>3279
                     /locus_tag="VFV25_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Orn/Lys/Arg decarboxylase N-terminal
                     domain-containing protein"
                     /protein_id="HEY1392113.1"
                     /translation="MLRFHFPIVIIDEDFRSENASGLGIRALAEAIEKEGFEVLGVTS
                     YGDLSQFAQQQSRASAFILSIDDEEFGGGSKEEVDAALKNVRAFVSEIRFKNAEIPIY
                     LYGETRTSQHIPNDVLRELHGFIHMFEDTPEFVARHIIREARSYLDSLAPPFFRALMD
                     YAQDGSYSWHCPGHSGGVAFLKSPIGQMFHQFFGENMLRADVCNAVEELGQLLDHTGP
                     VAASERNAARIFNADHCFFVTNGTSTSNKMVWHHTVAPDDVVVVDRNCHKSILHAIIM
                     TGAIPVFLTPTRNHLGIIGPIPKSEFEPATIRRKIAANPLLKGVDADRVKPRILTITQ
                     STYDGVLYNTETIKGMLDGWIDTLHFDEAWLPHAAFHKFYGTYHAMGKSGRGRPRESL
                     VFSTQSTHKLLAGISQASQVLAQDSQTRKLDRHLFNEAYLMHTSTSPQYSIIASCDVA
                     AAMMEPPGGTALVEESLLEALDFRRAMRKVDKEFGRDWWFKVWGPDRLAGEGLGRADD
                     WMIEADAKWHGFGSLAEGFNMLDPIKATIVTPGLDVSGKFAKTGIPASIVTKFLAEHG
                     VIVEKTGLYSFFIMFTIGITKGRWNTLLTALQQFKDDYDKNAPLWRILPEFCAQYPRY
                     DRMGLRDLCQSIHEM"
BASE COUNT          561 a         1107 c         1062 g          549 t
ORIGIN      
        1 ctcgccgccc ggctgctgga tggaggcggc gatcttgcgg atcgcgtcag ccgtcgcatc
       61 ggccaccgcc gtgatggcgg ccgcctcgcc ctgggccttg ttgatctcgg cctgcttggc
      121 accctcggag cgggcgatga aggcctcgcg ttcgccggtg gcgatattga tctgctcctg
      181 gcggcggccc tcggaggcgg caatcagcgc gcgcttctcg cgctcggcgg tgatctgggc
      241 ctgcatcgcg cgcaggatct cggccggtgg cgtcaggtcc ttgatctcgt agcgcagcac
      301 tttgacgccc caattgaggg cggcctcgtc gagcgcggtc accacctggg cattgatggc
      361 gtcgcgctcc tcgaaggtct tgtccagctc catcttgccg accaccgagc gcagcgtcgt
      421 ctgcgcgagc tgggtgatgg ccacgatgta gttgctggag ccgtagctgg cgcgcatcgg
      481 gtctgtcacc tggaaataga ggatgccgtc gacctgcagc tgggtgttgt ccttggtgat
      541 gcagacctgg ctgggcacat cgagcgggat ctccttcagc gagtggcggt aggcctggcg
      601 gtcgatgaag ggcacgagga agttcaggcc cggcgtgagc gtggcgtggt agcgccccag
      661 ccgctcgacc accaccgcat gctgctgcgg cacgactgtg acggacttga tcacgaagat
      721 ggccgcgatg acggccagca cgagtgcgac ttccatggat cctccttgat tcggattggt
      781 acgcaggcag cgcgcagcct caacgcccca gcagcagctg gttgccttcg atggcctgga
      841 tgaagtgctc gccaggcgcc ggttccccgt cgcccacata gcgcgccgac caggccgcgc
      901 cgcggtaatg gatacgcgcc gtgccatcgg agttccattg ctggacctgc acccgctcgc
      961 cgatgtcgag attcatgtcg cggttcttgg cggccggcgc cttggcgggc aggcggctgc
     1021 gcacgagata gcaggcggcc acggcgccgc cgccgaccag ggccgcggcg accagctcgg
     1081 cctcggtgcc caggcccagg tgggcggcga tggcgcctgc ggacaggccc agggccagca
     1141 tcagcagata gaaggtcccg gtggccagct ccagcgccac cgccgcgccg gcgacgaccc
     1201 accacaacat tgcatccgtc attgcctgtg gtcctccttg gggccggcag tgtacgctgc
     1261 acctgctcac agattcacaa aggcagcacc agggcgtcac gctctggccc tacacttcgc
     1321 gctttccttt ttcttccacg gcccgtccgg aggccccagc atgctgcgct ttcactttcc
     1381 catcgtgatc atcgacgagg acttccgctc ggagaacgcc tcgggcctgg gcatacgcgc
     1441 gctggccgag gccatcgaga aggagggctt cgaagtgctg ggcgtcacga gctacggcga
     1501 cctgtcgcag ttcgcccagc agcaaagccg cgccagcgcc ttcatcctgt cgatcgacga
     1561 cgaggagttc ggcggcggct ccaaggaaga ggtcgacgcg gcgctgaaga acgtgcgcgc
     1621 cttcgtcagc gagatccgct tcaagaacgc cgagatcccg atctacctct acggcgagac
     1681 acgcacctcg cagcacatcc ccaatgacgt gctgcgcgag ttgcacggct tcatccacat
     1741 gttcgaggac acgccggagt tcgtggcacg ccacatcatc cgcgaagcgc gcagctacct
     1801 cgactcgctg gcgccgcctt tcttccgcgc gctgatggac tacgcgcagg acggctccta
     1861 ctcctggcac tgtccggggc attccggcgg cgtggccttc ctgaagagcc cgatcggcca
     1921 gatgttccac cagttcttcg gcgagaacat gctgcgcgcc gacgtctgca atgcggtgga
     1981 ggagctcggt cagctgctgg accacaccgg cccggtggcg gccagcgagc gcaatgcggc
     2041 gcgcatcttc aacgccgacc attgcttctt cgtcaccaac ggcaccagca ccagcaacaa
     2101 gatggtctgg caccacacgg tggcgccgga cgacgtggtg gtggtggacc gcaactgcca
     2161 caagtccatc ctgcacgcca tcatcatgac cggggccatc cccgtgttcc tgacgcccac
     2221 gcgcaaccac ctgggcatca tcggaccgat ccccaagagc gagttcgagc cggcgacgat
     2281 ccggcgcaag atcgccgcca acccgctgct caagggcgtg gatgcggaca gggtcaagcc
     2341 gcgcatcctg accatcacgc agagcaccta cgacggcgtg ctctacaaca ccgagacgat
     2401 caagggcatg ctcgacggct ggatcgacac cctgcatttc gacgaggcat ggctgccgca
     2461 cgccgccttc cacaagttct acggcaccta ccatgcgatg ggcaagagcg ggcgcggccg
     2521 gccgcgcgaa tcgctggtgt tctcgaccca atccacgcac aagctgctgg ccggcatcag
     2581 tcaggcctcg caggtgctgg cgcaggactc gcagacgcgc aagctggatc ggcacctctt
     2641 caacgaggcc tatctgatgc acacctcgac cagcccgcag tattcgatca tcgcgagctg
     2701 cgacgtggcc gccgcgatga tggagccgcc aggcggcacg gccctggtcg aggaaagcct
     2761 gcttgaggcg ctggatttcc gccgcgcgat gcgcaaggtc gacaaggagt tcggccgcga
     2821 ctggtggttc aaggtctggg gaccggaccg gctggccggc gagggcctgg gccgcgccga
     2881 cgactggatg atcgaggccg acgcgaagtg gcatggcttc ggcagcctgg ccgaaggctt
     2941 caacatgctg gacccgatca aggccaccat cgtgacgccg ggcctggacg tgtcgggcaa
     3001 gttcgccaag accggcatcc cggccagcat cgtcaccaaa ttcctggccg agcacggcgt
     3061 gatcgtcgag aagacggggc tctattcctt cttcatcatg ttcacgatcg ggatcaccaa
     3121 ggggcgctgg aacacgttgc tgaccgcgct gcagcagttc aaggacgact acgacaagaa
     3181 cgcgccgctg tggcgcatcc tgccggagtt ctgcgcccag tatccgcgct acgaccgcat
     3241 gggcctgcgc gacctgtgcc agagcatcca cgagatgta
//
LOCUS       DASUCY010000099         2681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1142618, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000099 DASUCY010000000
VERSION     DASUCY010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142618"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2164
                     /locus_tag="VFV25_01995"
     CDS             <1..2164
                     /locus_tag="VFV25_01995"
                     /inference="COORDINATES: protein motif:HMM:TIGR03505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HEY1392114.1"
                     /translation="LQAVSPSEARQVIQAQSADFGAYRQHLAGKVAATETTASAQKSE
                     QGAGEAGADEYKVHSGDTLSRIASRAQRPGISLDQMLVGLYRGNPQAFLGDNMNRLKS
                     GVVLSVPSVEKLQAVSPSEARQVIQAQSADFGAYRQHLAGKVAATETTASAQKAGGQV
                     QGEVQDRKQAAATAPDKLTLSKGSAAAGAAAPEQKLAEERAKKDAANRVAELSKNVED
                     LKKLQSAGTAPAAKTAPAPKQAPGVAVPAAVGMAPVVSPPPAPAPAKPPAAAASTPAV
                     VATAASTPPAPIKAQAPAASAPAVVAAAATPASAPAAAAASAAVAATPAPAPRPPAPA
                     PVPAPAPAEEPGFFANLLDNPLVLPAVGAFALVAGGFGWYRMRAQKKQESGETSFLES
                     RLQPDSFFGASGGQRVDTRDATGVPSSMSYSLSQLDAIGDVDPVAEADVYLAYGRDLQ
                     AEEILKEAMRSAPDRLAIRTKLLEVYAKRRDTKGFELLANQLYSLTGGQGEDWAKTQE
                     LGLSIDPDNSLYRPGGHPASAVPGAVAGAGEVLGASTMPQSVLPSPSRFEAGLSGIDA
                     PDSGNVDLDLDLPAAASKPAARSSGALGKAPTPFIAPSFDAPATADAGAAKFDLPTIP
                     LRAEAHAASGPLDFDMGGLDLDLPSAPASSRAPAAEHFSLDVGNEAGDALARKLELAE
                     EFRQIGDTEGARDLLKEVVAQASGSLKTKAQGMLDALA"
     gene            2196..>2681
                     /gene="truA"
                     /locus_tag="VFV25_02000"
     CDS             2196..>2681
                     /gene="truA"
                     /locus_tag="VFV25_02000"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEY1392115.1"
                     /translation="MRVALGVSYRGQAYLGWQSQPGGRTVQDHLEAALARFAAAQSLR
                     SICAGRTDAGVHACNQVVHFDTELQRADYSWVRGTNTYLPPDIAVQWCRPVGEGFHAR
                     NSALGRRYVYVLLESAVRPSLEAGTVGWVFRPLDADAMRAAAGHLIGEHDFSAFRAAE
                     CQ"
BASE COUNT          413 a          956 c          923 g          389 t
ORIGIN      
        1 gctgcaggcc gtctcgccca gcgaggcgcg ccaggtgatc caggcgcaga gcgccgattt
       61 cggcgcctat cggcagcatc tggccggcaa ggtggcagcc accgagacca cggcctcggc
      121 ccagaagagt gaacagggcg ctggcgaggc cggcgccgac gaatacaagg tgcattccgg
      181 cgacacgctg tcgcgcatcg cgagccgcgc gcagcgcccc ggcatttcac tggaccagat
      241 gttggtgggt ttgtaccgcg gcaatcccca ggccttccta ggcgacaaca tgaaccggct
      301 gaagtccggc gtcgtgctgt cggtgcccag cgtcgagaag ctgcaggccg tctcgcccag
      361 cgaggcgcgc caggtgatcc aggcgcagag cgccgatttc ggcgcctatc ggcagcatct
      421 ggccggcaag gtggcagcca ccgagaccac ggcctcggcc cagaaggccg gcggccaggt
      481 gcagggtgag gtgcaggacc gcaagcaggc agctgcgaca gcgccggaca agctcacgct
      541 cagcaagggc agtgccgccg ctggtgctgc ggctcccgag cagaagctgg ccgaggaacg
      601 cgcgaagaag gatgctgcca atcgcgtggc cgagctgtcc aagaacgtcg aggatctgaa
      661 gaagctgcag agcgccggca cggcccccgc ggcaaagacg gcgcctgcac ccaagcaggc
      721 cccgggcgtt gcggttccgg ccgccgtcgg catggccccg gttgtgtcgc cccctccggc
      781 gcccgcgccc gccaagccgc cggcggcagc ggcaagcacc cctgcagtag tagcaactgc
      841 tgcatcgact cccccggcgc cgattaaagc ccaggcgccc gccgcttctg caccggcggt
      901 cgttgctgcg gcagcgaccc ctgcctctgc tcccgctgct gccgcggcgt cggccgcagt
      961 cgccgctacg ccggctccgg cgccccgtcc gcccgcgccg gctccagttc cggccccggc
     1021 cccggcggaa gagcccggct tcttcgccaa cctgctcgac aacccgctgg tgttgccggc
     1081 tgtaggggcc ttcgcgctgg tggccggcgg tttcggctgg taccgcatgc gcgcccagaa
     1141 gaagcaggag agtggcgaaa cctctttcct cgaaagtcgg ctgcagccgg attccttctt
     1201 tggcgccagc ggcggccagc gcgtcgacac gcgcgacgcc accggcgtgc cttcgtcgat
     1261 gagctattcg ctcagccagc tcgatgcgat cggcgatgtc gaccctgtgg ccgaggcgga
     1321 cgtctacctc gcctatgggc gcgacctgca ggccgaggaa atcctcaaag aagcgatgcg
     1381 cagcgcaccg gatcgcctgg cgattcgtac caaactgctc gaggtctatg ccaagcgtcg
     1441 cgacaccaag ggcttcgagc tgctggcgaa tcagctgtat tcgctgaccg gcgggcaggg
     1501 cgaggactgg gccaagacgc aggagctggg cctgtccatc gacccggaca acagcctgta
     1561 caggccgggc gggcatccgg cctcggcggt tccgggcgcg gtcgccgggg ccggcgaagt
     1621 cctgggcgcc agcaccatgc cccagtccgt gctgccttcc ccgtcccgct tcgaagccgg
     1681 gctttccggc atcgacgcgc cggattccgg caacgtggat ctcgatctgg acctgccggc
     1741 cgcggcatcg aagccggccg cgcgttcgtc tggcgctctc ggaaaagccc cgacgccgtt
     1801 catcgcgccg agctttgacg cgcccgcgac cgccgatgcc ggagctgcca agttcgactt
     1861 gccgaccata cccctccgcg ccgaggccca tgcagcctcc ggcccgctgg acttcgacat
     1921 gggcgggctc gacctggatc tgccgagcgc ccccgcaagc tcgcgtgcgc cggccgccga
     1981 gcatttcagc ctcgatgtcg gaaatgaggc gggcgatgcg ctggcgcgca agctcgaact
     2041 cgccgaggag ttccgtcaga tcggcgacac cgaaggtgcg cgcgacctgc tcaaggaagt
     2101 cgtcgcccag gccagcggca gcctcaagac caaggcccag ggcatgctcg acgccctggc
     2161 ctgagcggcc gggcgtctga gaggaggccg ccgccatgcg ggtggccctg ggagtgagtt
     2221 atcgcgggca ggcctatctg ggctggcaaa gccagcccgg cgggcgcacg gtgcaggatc
     2281 atctggaggc cgcgctggcg cgctttgcgg cggcccagtc gctgcgcagc atctgcgccg
     2341 gccgcaccga tgccggcgtg catgcctgca accaggttgt gcacttcgac accgaactgc
     2401 agcgcgcgga ttattcctgg gtgcgcggca ccaacaccta ccttccgcct gacatcgccg
     2461 tgcaatggtg ccggccggtc ggcgagggtt tccatgcccg caacagcgcg ctggggcggc
     2521 gctatgtcta tgtgctgctc gaatccgccg tgcgcccctc gctggaggcg gggacggtcg
     2581 gctgggtgtt ccggcccctg gatgccgatg cgatgcgcgc cgccgccgga catctgatcg
     2641 gcgagcacga cttcagcgcc tttcgcgctg ccgaatgcca g
//
LOCUS       DASUCY010000100         3301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_259009, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000100 DASUCY010000000
VERSION     DASUCY010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259009"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2166)
                     /locus_tag="VFV25_02005"
     CDS             complement(<1..2166)
                     /locus_tag="VFV25_02005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY1392116.1"
                     /translation="MAVFAAVIGLQVLVAALSIDLLSAVRAYVTGESLYSKGQKDAQI
                     YLLDYADFQKEEDYQRFLRALELPLGDRAAREELQKPQPDLGRVRQGFLAGGNHPDDI
                     DGLIRLFNWFHRVPFMAAAIQTWTEGDSVIEQMRVLVERAREQVKAGTLDAQAAREMR
                     MQALGLNQRMTELERRFSEQLGAASRQTKGLLLGLNLSFAALLSLAGMRFVRQSLRER
                     ALAEAEVRRRQESLQRMLDSAAEGLYGVDTEGKCTFVNRSALAMLGYEQESDLVGRNI
                     HALIHHTYPDGRPYPARDCKLSRAERQGQHAHVVDEVFWRRDGRSFPVECWSHPVVQD
                     GQVQGMVVTFFDITERVRAQAALRESEARLSRLVDSVADGVITIDARERVILFNRAAE
                     KLFRVRAAEIVGRPLERLIPERLRPRHHAGVQQFARGEAENRIVNGLHELVGLRANGE
                     EFPIEASLSKLETEQGLLMTVVLRDVSEQRATLKERQAREELEASNRAKTQFLSRMSH
                     ELRTPLNAVLGFAQLMRMDRAHTLATDQLARVHHIEHAGNHLLALVNDVLDLSRVESG
                     QMSLSVEPVDASAVAEEAIALVSPLAAEVGVKVGSSSSPRQGAQASMTGADEQATAAA
                     DEYWVLADRVRLRQVLVNLLNNAVKYNRPGGQVMLSHERSSGWCQIVVADTGKGMTAE
                     QVNRLYEPFNRLGAELSGIEGTGIGLVLTRHLVELMGGDI"
     gene            complement(2326..3132)
                     /locus_tag="VFV25_02010"
     CDS             complement(2326..3132)
                     /locus_tag="VFV25_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010110832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEY1392117.1"
                     /translation="MSDAFVRDGELTDIHSYPAWAQELVDSCAEARDQVARHELFQLM
                     RDAALNNNQMSTFLVGVWPVIEQFPQYMAQNLLKVQFGRTRGHDEARRYLIRNIRVEQ
                     NHADHWLEWAQASGVSRDDLLHGEVPPETQALAHWCWHTCERDSLAAGMAATNYAIEG
                     VTGDWSGLVCSADTYEQSFPAEVRSKAMKWLKLHAKYDDTHPWEALEIICALMGTKPT
                     ARGVELIRSRVVTSYNYMRLTLDHCLDAEHIVSQPLPLVSLDYPAERRRA"
BASE COUNT          512 a         1138 c         1082 g          569 t
ORIGIN      
        1 gatgtcgccg cccatcagct cgaccagatg gcgcgtgagc accaggccga tgcccgtgcc
       61 ttcgatcccg gatagctcgg cgccgaggcg attgaagggt tcgtagaggc ggttcacctg
      121 ctcggccgtc atgcccttgc ccgtatcggc gacgacgatc tgacaccagc cgctgctgcg
      181 ctcgtggctc aacatcacct ggccgcctgg gcggttgtac ttgactgcgt tgttcagcag
      241 gttgaccagg acctggcgca ggcgcacgcg gtcggccagg acccagtatt catcggccgc
      301 tgccgtcgcc tgctcgtccg cgcccgtcat ggaagcctgc gcgccttgcc gcggcgacga
      361 agagctgccg accttgacgc cgacttcggc agccaggggc gaaaccaggg cgatcgcctc
      421 ttcggccacc gcgctcgcgt cgacaggctc caccgaaagg ctcatctgcc ccgattccac
      481 gcgggacaga tccaggacgt cgttcaccag cgccagcaga tggtttccgg cgtgctcgat
      541 gtgatgcacc ctggcgagct ggtcggtggc cagggtgtgc gcgcgatcca tacgcatcag
      601 ctgggcgaac ccgagcacgg cgttcagggg cgtgcgcagt tcatggctca tgcgcgacag
      661 gaactgggtc ttggcgcgat tcgaggcctc gagctcttcg cgcgcctgcc gctccttcag
      721 cgtcgcgcgc tgctcgctca cgtcgcgcag cacgaccgtc atcagcaggc cctgctcggt
      781 ctcgagcttg gagagcgagg cctcgatcgg gaactcctcg ccgttcgcgc gcagcccgac
      841 cagctcgtgc agcccgttga cgatcctgtt ttcggcctcg ccgcgagcaa actgctgcac
      901 gccggcatga tgccgggggc gcaggcgctc cgggatgagg cgctccagcg ggcggccgac
      961 gatctcggcc gcccgcacgc gaaacagctt ttcggccgcg cggttgaaca ggatcaccct
     1021 ctcgcgggcg tcgatcgtga tcacgccgtc ggcaacgctg tcgaccagcc gggacaggcg
     1081 cgcctcgctc tcgcgcagcg cggcctgggc gcgcacccgc tcggtgatgt cgaagaaggt
     1141 gacgaccatg ccctgcacct gcccgtcctg caccaccgga tgcgaccagc attcgaccgg
     1201 gaaggatctg ccgtcgcggc gccagaacac ctcgtcgacc acatgtgcgt gctgcccctg
     1261 cctttcggcc cgggataatt tgcagtcgcg ggcagggtag gggcgcccgt cgggataggt
     1321 gtggtggatc agggcatgga tgttgcgccc gaccaggtcc gactcctgct catagcccag
     1381 catcgccagc gcagagcgat tgacgaaggt gcacttgccc tccgtgtcca cgccgtagag
     1441 cccttcggcc gcggagtcga gcatgcgctg cagcgattcc tggcggcgcc gcacctcggc
     1501 ttccgccaag gcgcgttcac gcaggctctg gcgcacgaag cgcatgccgg ccaaggagag
     1561 cagggcggcg aaggagagat tcaggcccag cagcagcccc ttggtttgcc gcgacgccgc
     1621 tccgagttgc tccgagaacc ggcgctccag ctccgtcatg cgctggttga ggcccagggc
     1681 ctgcatgcgc atttcgcggg ccgcctgtgc gtcgagcgtg cccgccttga cctgctcgcg
     1741 cgcgcgctcg accagcacgc gcatctgctc gatcacgctg tcgccctcgg tccaggtctg
     1801 gatcgccgcg gccatgaagg ggacacggtg gaaccagttg aagagccgaa tcaggccatc
     1861 gatgtcgtcg gggtggttgc cgcccgccag aaagccctgg cgcactcggc cgaggtcggg
     1921 ttgcggcttc tgcaactcct cgcgcgccgc gcggtcgccg agcggcaatt cgagcgcgcg
     1981 cagaaagcgc tggtagtcct cttccttctg gaagtcggcg tagtcgagca ggtagatctg
     2041 cgcgtccttc tgccccttgg agtacaggct ctcgcccgtc acgtaggcgc gcaccgccga
     2101 cagcaggtcg atgctcagcg cagccaccag cacctgcagg ccgatgacgg ccgcaaaaac
     2161 ggccacgacc ggccaggacc ggcggcgtcg tatggcttgg ccgtttgcgc gcatgcccgg
     2221 cttctaagca gcggacttct tcatccagcg atcctcctct tcgcggcgaa ccgcagcggc
     2281 aagccgggca tgccgcctcg attcagcccg gcacatacct ggggctcagg cgcgcctgcg
     2341 ctcggcgggg tagtccagcg aaaccagcgg cagcggctgc gaaacgatgt gctcggcatc
     2401 caggcagtgg tctagcgtca gccgcatgta gttgtagctg gtcacgacgc gcgagcggat
     2461 cagctcgacg ccgcgcgcgg tcggcttggt gcccatcagc gcgcagatga tctccagcgc
     2521 ctcccaggga tgggtgtcgt cgtacttggc gtgcagcttc agccacttca tcgccttgct
     2581 ccggacctcg gccgggaagc tttgttcgta ggtatccgcc gagcagacca gacccgacca
     2641 gtcgccggtc acgccctcga tcgcgtagtt ggtggccgcc atgcccgcgg ccagcgaatc
     2701 gcgctcgcag gtgtgccagc accagtgggc cagcgcctgc gtctccggtg gcacctcgcc
     2761 gtgcagcagg tcgtcgcggc tgacgccgct ggcctgcgcc cattcgagcc agtggtcggc
     2821 atgattctgc tcgacgcgga tattgcggat gaggtagcgg cgcgcttcgt catggccgcg
     2881 ggtgcggccg aactgcacct tcagaaggtt ctgggccatg tattgcggga actgctcgat
     2941 gaccggccac accccgacga ggaaggtgct catctggttg ttgttgagcg cggcgtcgcg
     3001 cattagctgg aacagctcgt gccgggcgac ctgatccctc gcctcggcgc agctgtccac
     3061 cagctcctga gcccatgcgg gataactgtg gatgtcggtc agctcgccgt ctcgaacgaa
     3121 cgcgtcgctc atcgcccttc ctccctgatc cgtgacgcac cggggcgctt gcgcaccgcc
     3181 tccaagctca ccacagacga caaccacatc ccttgcgggt acaacagccg cttgaccggc
     3241 tgaagcattc tggaattgga agcgaagatc ggtctactga caagtcttac aggtcgtgcc
     3301 g
//
LOCUS       DASUCY010000101         2358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_194719, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000101 DASUCY010000000
VERSION     DASUCY010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194719"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..1093
                     /locus_tag="VFV25_02015"
     CDS             101..1093
                     /locus_tag="VFV25_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY1392118.1"
                     /translation="MQYRRLGRSGLQVSELSLGSWVTYANQVDTAAAREMLAAAFDAG
                     INFFDNAEVYANGQSETIMGQAFKQLGWSRLDYVVSTKFFWGLDRAKRGERPRVNGQD
                     TLNRKYLMQAMDGSLKRMQLDHIDLVFCHRPDPNTPIEETVWAMSDLIRQGKALYWGT
                     SEWSAAEIRAAWDIADKHHLHKPVMEQPEYNLFHRKRVEQEYARLYEDTGLGLTTWSP
                     LASGLLTGKYRQGLPAGSRGALENMAFLRDQLLDPEKNAAVEKLESIAHELGGNMAQL
                     AIAWINRNPRVSTVILGASKLAQLKDNLGALTLTPKLSPEVLARIDEICQPLAG"
     gene            complement(1100..1819)
                     /locus_tag="VFV25_02020"
     CDS             complement(1100..1819)
                     /locus_tag="VFV25_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392119.1"
                     /translation="MILSVCWLLFSICAAMAAEMLVARGAAAVALSYAPLSGDQTDSV
                     TYQRSQLTPGEPALRAVWAGTAAAGALLWAAATFDMGWFYLLGLLAWLAALAWDAWTW
                     ERAAASVKFVSWRRGWQHSTRRVPVAQVSEVHVVEKPSALNFGLFKLRYAACYVALQM
                     SDGKAIKLPRTATLGDLNRVEDYANFVRLQMQMVEDTRKRVAAEKRAEARRAGLPDPD
                     AERELRKRLAELRQAGAKSDE"
     gene            complement(1816..>2358)
                     /locus_tag="VFV25_02025"
     CDS             complement(1816..>2358)
                     /locus_tag="VFV25_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY1392120.1"
                     /translation="GQFPATLGDGSRWGLSIRYSMEGRDHGGALETAGGIAKALPWLT
                     QGGAGAFWVVSGDIYAPDFRFDAELAARFAANPGLDAHLWLVPNPPFHPAGDFGLGAD
                     GLGLAGAGPDGKRWTYGNLALIKPSLVRDIAPGTRAALGPLLYQGMNERRIAADIYTG
                     RWENIGTPAQLEALNRGLAA"
BASE COUNT          414 a          813 c          786 g          345 t
ORIGIN      
        1 gagggctgca tgggtcaata cctttaggct acgctgcgca gtctagcgtc gcccgcgatc
       61 ccgctggcct cacgcatcgc cgaatacgga gaacccctca atgcaatacc gccgcctcgg
      121 ccgttccggc ctgcaagtca gtgagctctc gctcggctcc tgggtgacct atgccaacca
      181 ggtcgacacg gccgccgcgc gcgagatgct ggccgccgcc ttcgacgccg gcatcaactt
      241 cttcgacaac gccgaggtct atgccaacgg ccagagcgag accatcatgg gccaggcctt
      301 caagcagctg ggctggtcgc ggctggacta tgtggtgtcg accaagttct tctggggcct
      361 cgaccgcgcc aagcgcggcg agcgtccgcg cgtcaacggc caggacacgc tgaaccgcaa
      421 gtacctgatg caggcgatgg acggctcgct caagcgcatg cagctcgacc acatcgacct
      481 cgtgttctgc catcgccccg atcccaatac gccgatcgag gagacggtgt gggccatgag
      541 cgacctgatc cgccagggca aggcgctgta ctggggcacc agcgaatggt cggccgccga
      601 gatccgcgcc gcttgggaca ttgccgataa gcaccatttg cacaagccgg tgatggagca
      661 acccgagtac aacctctttc accgcaagcg ggtggagcag gaatatgcgc ggctctacga
      721 ggacaccggc ctgggcctga ccacctggag cccgctggcc agcggattgc tgaccggcaa
      781 gtaccgccag ggtctgccgg ccggcagccg cggcgcgctg gagaacatgg ccttcctgcg
      841 cgaccaactg ctcgatcccg agaagaatgc ggcggtcgag aagctcgaat ccatcgcgca
      901 cgagctgggc gggaacatgg cgcagctggc gatcgcctgg atcaatcgca acccgcgtgt
      961 gagcaccgtg atcctgggcg ccagcaagct ggctcaactc aaggacaacc tcggcgcgtt
     1021 gactctgacg cccaagctca gccccgaggt gctggcgcgc atcgacgaga tttgccagcc
     1081 gctggcgggc tgagttcgcc tactcgtcgg acttggcgcc cgcctggcgc aactcggcca
     1141 ggcgcttgcg cagctcgcgc tccgcatccg ggtcgggcag gccggcgcgg cgggcttcgg
     1201 cccgcttctc ggccgcgacg cgcttgcgcg tgtcctcgac catctgcatc tgcagccgca
     1261 cgaagttcgc atagtcctcg acgcggttca gatcgcccag cgtggccgtg cgcggcagct
     1321 tgatggcctt gccgtcgctc atctgcaggg ccacatagca ggccgcgtag cgcaacttga
     1381 agaggccgaa gttcagggca ctgggttttt cgaccacatg cacttcgctg acctgggcaa
     1441 ccggcacgcg gcgcgtggaa tgctgccagc cgcggcgcca ggacacgaac ttgacgctgg
     1501 ccgcggcgcg ctcccaggtc caggcgtccc aggccagcgc ggccagccag gccagcaggc
     1561 ccaggaggta gaaccagccc atatcgaaag ttgcggcggc ccagagcagc gcgccggccg
     1621 ccgcagtgcc ggcccagacg gcgcgcagcg cgggctcgcc gggggtcaac tgggagcgct
     1681 ggtaggtgac cgagtcggtc tggtcgcctg acagcggcgc ataggacaac gccacggctg
     1741 ccgcgccgcg cgcaaccagc atctcggccg ccatggccgc gcagatgctg aacaacagcc
     1801 agcagacgga aaggatcatg ccgccaggcc ccgattcagg gcttcgagtt gcgcaggagt
     1861 gccgatattt tcccagcgac cggtgtagat gtctgcggcg atacggcgct cattcatgcc
     1921 ttgatacaaa agcggtccca aggccgcccg ggtgccgggg gcgatgtcgc gcaccagcga
     1981 cggtttgatc agcgccaggt tgccgtaggt ccagcgcttg ccgtccggcc ctgcgccggc
     2041 caggccgagc ccgtcggcac ccaggccgaa atcgccggcg ggatggaagg gcgggttggg
     2101 caccagccag agatgggcat cgaggccggg attggccgcg aagcgcgccg ccagctccgc
     2161 atcgaagcga aagtcgggtg catagatgtc gcccgacacg acccagaacg cacccgcccc
     2221 gccctgcgtg agccagggca gggccttggc gatccctccc gccgtttcca gcgcgccgcc
     2281 gtggtcgcgc ccctccatcg aatagcggat ggacaggccc cagcgcgagc catcgccgag
     2341 ggtggcaggg aactgccc
//
LOCUS       DASUCY010000102         2140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1094484, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000102 DASUCY010000000
VERSION     DASUCY010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094484"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2140)
                     /locus_tag="VFV25_02030"
     CDS             complement(<1..>2140)
                     /locus_tag="VFV25_02030"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEY1392121.1"
                     /translation="AGDAKPAGAKTDARAIAPPPSPPRINADAQFDLRIPEEKAAGRK
                     PRSLLQQLAAWRGQARAQLRDSQFGGVPLAGELALESHAARQRLLGHARLEAGGNQLT
                     LDGEIDATAAASDRWKLQVQAPKLDSLAPLLRLLQPGSPPQASAGALSAQAEAEGRWP
                     SLHSRGELQAQGLSLLSWSAAGVRANWDFGTTPEAAVNLQAEISQVALGQRKLDLLRA
                     QLSGTGRSHQASLRIEAPPLRPTNGASQGNGSPLDALAEQRLLALATASGGWSRDERT
                     ASLGWQGRVQQLELRALAPGAAQTGNPGTAWLHADPFELSWAGGADSQRLSVSPTRLS
                     VSDAALKLSRLEWSQRAGAAPRFDLQAELEPLKLAPLLARAQPDFGWAGDLTVDGHLA
                     LHSDAEGGFRADALLERSAGDLEVVDPAQLGAKPLRLGLSEARFALSANNGLWQFTQQ
                     LTGANLGAINGEQTVSTRRELSWPEPAAPLHGQLDIRVANLATWGAWVPPGWRLSGQL
                     ATQASVGGRFGAPEYTGHLSGSHIGVRNVLAGVNLSEGEVEIALQGDTARIERFNVRG
                     GQGQARLEGEASLGAAPQARLQLTADHFAVLSRVDRRLSLSGQAQLELQSKALKLDGR
                     LVADSGLVDISHGGAPSLADDVTVVRRGSGDAAAALSEPAKTAPRMSNVALNLKLDLG
                     PDFRLRGHGLDTLLRGELAVTTPGGLMNVKG"
BASE COUNT          254 a          767 c          787 g          332 t
ORIGIN      
        1 gcccttcacg ttcatcaggc cgccgggcgt cgtgactgcc agctcgccgc gcagcaaggt
       61 gtccaggcca tgcccgcgca ggcggaagtc cggccccagg tccagcttca ggttcagcgc
      121 gacattgctc atgcgcggcg ccgtcttggc cggttcggag agggcggccg cggcatcgcc
      181 gctgccccgg cgcacgaccg tcacgtcgtc tgcgaggctg ggcgcgccac cgtgcgagat
      241 gtcgacgagg ccgctgtcgg ccaccaaccg cccgtcgagc ttgagggcct tgctctgcag
      301 ctcgagctgg gcctggccgc tgagcgacag ccggcgatcg acgcggctca gcaccgcgaa
      361 gtgatcggcg gtgagttgga gccgcgcctg cggcgcggcg cccaggctgg cctcgccttc
      421 cagcctcgcc tggccctggc cgccgcgcac attgaagcgc tcgatgcgcg ccgtgtcgcc
      481 ctgcagcgcg atctcgactt cgccttcgct caggttcacg ccggccagca cgttgcgcac
      541 gccgatgtgg ctgccgctca ggtggccggt gtattcgggc gccccgaagc gcccgcccac
      601 gctggcctgc gtggccagct gcccggaaag ccgccagccc ggcgggaccc aggcgcccca
      661 ggtggcgagg ttggcgacgc ggatgtcgag ctggccgtgc agcggcgccg caggctcggg
      721 ccagctcagt tcgcggcgcg tgctcacggt ctgctcgcca ttgatggccc cgaggttggc
      781 cccggtcagc tgctgggtga attgccacag tccgttgttc gcgctgaggg cgaaccgggc
      841 ttcgctcagt cccaggcgca gcggcttggc gccgagctgt gcggggtcga ccacctcgag
      901 gtcgccggcg ctgcgctcca gcaaggcgtc ggcgcggaag ccgccttcgg catcgctatg
      961 cagcgcgaga tggccgtcca ccgtcagatc gccggcccag ccgaagtcgg gctgcgcgcg
     1021 ggccagcagc ggcgccagct tcagcggctc cagctcggcc tgcagatcga agcggggtgc
     1081 cgcgccggcg cgctgcgacc actcaaggcg gctcagcttg agcgcagcgt ccgagacgct
     1141 cagccgcgtc ggcgagacgc tcaggcgctg gctgtccgcg ccgccagccc agctcagctc
     1201 gaagggatcg gcatgcagcc aggccgtgcc gggattgccg gtctgcgccg cgccaggcgc
     1261 caatgcgcgc agctccagct gctgtacgcg cccctgccag cctaggcttg cggtgcgttc
     1321 gtcgcgcgac cagccgccgc tggccgttgc gagcgcgagc agccgctgct cggcgagggc
     1381 atccaggggc gatccattgc cttggcttgc accgttggtt ggccgcaatg ggggcgcttc
     1441 gatgcgcaag ctagcctgat gcgaacggcc tgtgccgctc agctgggcgc gcagcagatc
     1501 gagcttgcgc tgcccgagcg cgacttggct gatttccgcc tgcaggttga cggcggcctc
     1561 gggcgtcgtg ccgaagtccc agttggcccg tacgccggct gcgctccagg acagcaggct
     1621 caggccttgg gcctgcagct cgccgcggct gtgcagcgag ggccagcggc cctcggcctc
     1681 ggcctgggcg ctcagggccc cggcggaagc ctggggcggc gagccaggct gcagcaggcg
     1741 caacagcggg gcaaggctgt cgagcttggg ggcctgcacc tgcaacttcc agcggtcgct
     1801 ggcagcggcg gtggcgtcga tttccccgtc cagagtcaac tggttgccgc ccgcctccag
     1861 cctggcgtgg ccgagcaggc gctggcgtgc cgcgtggctt tccagcgcga gctcgccggc
     1921 cagcggcaca ccgccgaact ggctgtcgcg cagctgcgct ctggcctggc cgcgccaggc
     1981 cgcgagctgt tgcagcaggc tgcgcggctt gcgtccggct gccttttctt cggggatgcg
     2041 caggtcgaat tgcgcgtccg cgttgatgcg cggcggcgat ggtggtggcg cgatagccct
     2101 ggcgtcggtc tttgcaccgg ctggctttgc atcgccggcc
//
LOCUS       DASUCY010000103         4919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1030181, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000103 DASUCY010000000
VERSION     DASUCY010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4919
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030181"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VFV25_02035"
     CDS             complement(<1..273)
                     /locus_tag="VFV25_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY1392122.1"
                     /translation="MYEAQFGLKEPPFQLNPDPAFYFDSRGHGNALAYLKFGIYQGEG
                     FIVVTGDIGAGKTTLVRTLLAGLDTEQIVAAQVVSTQLDPAELLQAV"
     gene            complement(460..1230)
                     /gene="hisF"
                     /locus_tag="VFV25_02040"
     CDS             complement(460..1230)
                     /gene="hisF"
                     /locus_tag="VFV25_02040"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEY1392123.1"
                     /translation="MLAKRIIPCLDVTGGRVVKGVNFVELRDAGDPVEIAARYNEQGA
                     DELTFLDITATSDGRDLILPIIEAVASQVFIPLTVGGGVRTVEDVRRLLNAGADKVSF
                     NSAAVANPQVIRDASEKYGAQCIVVAIDAKARIDQLSGPGWEVYTHGGRKNTGLDAVA
                     WARQMAEFGAGEILLTSMDRDGTKSGFDLALTRAVSDAVSVPVIASGGVGNLEHLVDG
                     IRLGAADAVLAASIFHYGEYTVGQAKALMAERGIPVRL"
     gene            complement(1278..2027)
                     /gene="hisA"
                     /locus_tag="VFV25_02045"
     CDS             complement(1278..2027)
                     /gene="hisA"
                     /locus_tag="VFV25_02045"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019699801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEY1392124.1"
                     /translation="MLLIPAIDLKDGQCVRLKQGDMNDSTTFGEDPAAMARRWLDAGA
                     RRLHLVDLNGAFAGKPVNEAAIKAILAEVGDEIPVQLGGGIRDLDTIERYLDDGLSYI
                     IIGTAAVKNPGFLRDACTAFGGHIIVGLDAKDGKVATDGWSKLTGHEVVDLAKKFEDY
                     GVEGVIYTDIGRDGMLSGINIDATVKLAQALSIPVIASGGLSDIADIEKLCAVEAEGI
                     EGVICGRSIYTGALDFAAAQKRADQLNGQAS"
     gene            complement(2089..2745)
                     /gene="hisH"
                     /locus_tag="VFV25_02050"
     CDS             complement(2089..2745)
                     /gene="hisH"
                     /locus_tag="VFV25_02050"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEY1392125.1"
                     /translation="MTRTVAVVDYGMGNLRSVSQAVLHAAAGTGVDVVVTAEPEVVRA
                     AERIVLPGQGAMRDCMRELREAHEGALLQSVLEAAAAKPLMGVCVGMQMLLDRSEEQD
                     TPGLGLIPGQVKRFRLEGLSQPDGSRYKVPQMGWNRVHQARPHPIWAGVPDEAWFYFV
                     HSYYAAPAQDVHSTGETEYGARFTSAVARDNIFATQFHPEKSAAHGLALYRNFLTWKP
                     "
     gene            complement(2747..3343)
                     /gene="hisB"
                     /locus_tag="VFV25_02055"
     CDS             complement(2747..3343)
                     /gene="hisB"
                     /locus_tag="VFV25_02055"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEY1392126.1"
                     /translation="MSAPRIAEITRNTKETQIRVRVNLDGTGEARLSTGIGFFDHMLD
                     QIARHGLIDLEIEAKGDLHIDGHHTVEDVGITLGLAVAQAVGDKKGLTRYGHAYVPLD
                     EALSRVVVDFSGRPGIEMDVAFKAGMIGALDTQLVFEFFQGFANHALVTLHIDNLKGH
                     NAHHQCETIFKAFGRALRMAMTLDPRSAGVIPSTKGSL"
     gene            complement(3343..4428)
                     /gene="hisC"
                     /locus_tag="VFV25_02060"
     CDS             complement(3343..4428)
                     /gene="hisC"
                     /locus_tag="VFV25_02060"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEY1392127.1"
                     /translation="MSDLQMRMQRVIRQDVQSMHGYAIQPSAGLVKLDAMENPFRLPV
                     GLQQALGERLGRVAINRYPTACVGDVIAALAQYVKLPAGCKLMLGNGSDELISLLAMA
                     CDVPGATILAPVPGFVMYEMSARLQGLSFVGVPLTANFELDESAMLAAIEQHRPAVTY
                     IAYPNNPTANLFDDAVIDKIVAAVGRQNGLVVFDEAYQPFSSRTWMGRMASHEHVLVM
                     RTLSKFGLAGVRLGYMAGVAALVAEIDKVRPPYNVSVLNAEATLFALEHADEFARQAA
                     ILREQRERLLVALRAMPGVTPFPSEANMILVRVPDSKAAFEGMKQRGVLVKHIAGLHP
                     LLANCLRLTVGTPDENTLMIDALKASL"
     gene            complement(4425..>4919)
                     /locus_tag="VFV25_02065"
     CDS             complement(4425..>4919)
                     /locus_tag="VFV25_02065"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEY1392128.1"
                     /translation="DAGYIARVKAEIERLLPGMPRRDVIRASLEGRGALIHTRSMEEA
                     CEISNRIAPEHLEVSSREPQRWEPLLKHAGAIFLGAYTSESLGDYCAGPNHVLPTSGT
                     ARFSSPLGVYDFQKRSSLIEVSEAGAQVLGEIAAELAYGEGLQAHAKAAEFRMAKNKK
                     GVTQ"
BASE COUNT          790 a         1641 c         1641 g          847 t
ORIGIN      
        1 cacggcctgc agcagttcgg ccggatcgag ctgggtgctg acaacctggg ccgcgacgat
       61 ctgctcggtg tccaggccgg ccagcagggt gcgcaccagc gtggtcttgc cggcgccgat
      121 gtcgccggtc acgacgatga aaccctcgcc ctgatagatg ccgaacttca gataggcgag
      181 cgcattgccg tgcccccggc tgtcgaaata gaaggccggg tcggggttga gctggaaggg
      241 gggttccttc aggccgaatt gagcttcgta catcggggcg cgccagcggg ctgtggcttt
      301 cgtttgcagg ccggggccgg cggccacggc ggccgcgctg cccgtccacg cagcttagcc
      361 gcgccgaggc cctgcaacga gcccaccccc ctcgaatgaa cgagaaggca gctcgccggc
      421 ggccctgtca tgcccctgaa aaaggggggg ccgcgagcgc tacagccgca ccggtatgcc
      481 gcgctcggcc atcagggcct tggcttggcc cacggtgtac tcgccgtagt ggaagatgct
      541 ggctgccagc accgcatcgg cggcgcccag acgtatgccg tcgaccaggt gctccaggtt
      601 gccgacgccg cccgaggcga tcacgggcac gctgacggca tcgctcacgg cacgcgtgag
      661 cgcgaggtcg aagccgctct tggtgccgtc gcggtccatg ctggtgagca ggatctcgcc
      721 ggcgccgaac tcggccatct gcctggccca ggccacggca tccagccctg tgttcttgcg
      781 cccgccatgg gtgtagacct cccagcccgg gccgctgagc tggtcaatgc gggcctttgc
      841 gtcgatcgcc accacgatgc attgcgcacc gtatttctcg gacgcatcgc gtatcacctg
      901 cggattggcc accgcggccg aattgaagct gaccttgtcg gcgcccgcgt tgagcaagcg
      961 ccgcacgtcc tcgacggtgc gcacgccacc gcccaccgtg agcggaatga agacttggct
     1021 agccacggcc tcgatgatgg gcaggatcag gtcgcgcccg tcgctggtcg cggtgatgtc
     1081 gaggaaggtc agctcgtcgg cgccctgctc gttgtagcgc gcggcgattt ccaccggatc
     1141 gccggcgtcg cgcagttcga cgaagttgac gcccttgacg acgcgaccgc cggtcacgtc
     1201 caggcaggga atgatgcgtt tggccagcat ggcagatgcg gccgggccgc gtatgaatga
     1261 aggatgggag tcgccgttca ggaggcctgg ccgttgagct ggtcggcgcg cttctgggcc
     1321 gccgcgaagt ccagcgcgcc ggtgtagatc gagcgcccgc agatcacgcc ctcgatgccc
     1381 tcggcttcca cggcgcagag tttctcgatg tcggcgatgt cgctcaagcc tcccgaggcg
     1441 atgaccggga tgctcagcgc ctgcgccagc ttgacggtgg catcgatgtt gatgccgctc
     1501 agcatgccgt cgcggccgat gtcggtatag atcacgcctt cgacgccgta atcctcgaac
     1561 ttcttcgcca gatccaccac ctcgtggccg gtgagcttgc tccagccgtc ggtggcgacc
     1621 ttgccgtcct tggcgtccag gccgacgatg atgtggccgc cgaaggccgt gcaggcatcg
     1681 cgcaggaagc ccgggttctt gaccgccgcc gtgccgatga tgatgtagct caggccatcg
     1741 tcgagatagc gctcgatggt gtcgaggtcg cggatgccgc cgcccagctg caccgggatt
     1801 tcatcgccca cctcggccag gatggccttg atggccgcct cgttgaccgg cttgccggcg
     1861 aaggcgccgt tcaggtccac cagatggagc cgccgcgccc cggcatcgag ccagcgccgc
     1921 gccattgcgg ccgggtcttc gccgaaggtg gtggagtcgt tcatgtcgcc ttgtttgagg
     1981 cgcacgcatt gaccgtcttt caggtcgatc gccgggatca gcagcatggc tgttgaagcg
     2041 cagtaaagaa aaaggaggga agagcgaaag gaaaggacga gaaaagactc agggcttcca
     2101 ggtcaggaag ttgcggtaca gggccaggcc atgtgctgcg cttttctcgg ggtgaaactg
     2161 ggtggcaaaa atattatcgc gagccaccgc cgaggtaaag cgcgcgccgt actcggtttc
     2221 accggtgctg tgaacgtcct gtgcgggtgc ggcgtagtag ctgtgcacga aatagaacca
     2281 ggcctcgtcg ggcacgccgg cccagatggg atgcggccgg gcctggtgca cgcgattcca
     2341 gcccatctgc ggcaccttgt agcggctgcc gtcgggctgg ctcaagcctt cgagccggaa
     2401 gcgcttcacc tggccgggaa tcagtcccag gcccggcgtg tcctgctcct cgctgcggtc
     2461 gagcagcatc tgcatgccca cgcacacgcc catcagcggc ttggctgccg ccgcttcgag
     2521 cacgctttgc agcagcgccc cctcatgggc ctcgcgcagc tcgcgcatgc aatcgcgcat
     2581 tgcgccctgg cccggcagca cgatgcgctc ggcagcgcgc accacctcgg gctcggccgt
     2641 cacaaccacg tccacgccag tgcccgcggc cgcatgcagc acggcctgcg acaccgagcg
     2701 caggttgccc atgccgtagt cgacgactgc aactgtgcgg gtcatctcag agactcccct
     2761 tggtcgaggg gatgacgccg gccgaacggg gatccagcgt catcgccatg cgcagcgcgc
     2821 ggccgaaggc cttgaagatg gtttcgcact ggtgatgcgc gttgtggccc ttgaggttgt
     2881 cgatgtgcag cgtcaccagc gcgtggttgg cgaagccctg gaaaaactcg aagacgagct
     2941 gggtgtcgag ggcgccgatc atgccagcct tgaaggccac gtccatctcg atgccggggc
     3001 ggccggagaa gtcgaccacg acgcgcgaca gcgcctcgtc cagcggcaca taggcatggc
     3061 cgtagcgcgt gagccccttc ttgtcgccca ccgcctgggc cacggccaag ccgagcgtga
     3121 tgcccacgtc ttccaccgtg tggtggccgt cgatgtgcag atcgcccttg gcctcgatct
     3181 cgaggtcgat caggccatgc cgcgcgatct ggtcgagcat gtggtcgaag aagccgatgc
     3241 cggtgctgag cctggcttcg ccggtgccgt cgaggttgac gcgcacgcgg atctgcgttt
     3301 ccttggtgtt gcgggtgatt tccgcaatgc ggggggcgct catcacaggc tcgctttcaa
     3361 ggcatcgatc atcaaggtat tttcgtccgg cgtgccaacc gtcaaacgca ggcagttggc
     3421 cagcaaggga tgcaggccag cgatgtgctt gaccagcacg ccgcgctgct tcatgccctc
     3481 gaaggcggcc ttggaatcgg gcacgcgcac gagaatcata ttggcctcgc tcgggaaagg
     3541 cgtcacgccg ggcatcgcac gcagcgcaac cagcagccgc tcgcgctgct cgcgcaagat
     3601 ggccgcctgg cgcgcgaact cgtccgcatg ctccagcgcg aacagcgtcg cctcggcatt
     3661 gagcacgctg acgttgtagg gcgggcgtac cttgtcgatc tcggcgacca gcgccgctac
     3721 gccggccatg tagcccaggc gcacgccggc caagccgaac ttgctgagcg tgcgcatcac
     3781 cagcacatgc tcgtggcttg ccatgcggcc catccacgta cgcgacgaga acggctgata
     3841 ggcttcatcg aacaccacca ggccgttctg cctgcccacg gcagcgacga tcttgtcgat
     3901 caccgcgtcg tcgaacagat tggccgtcgg attgttcgga taggcgatgt aggtgaccgc
     3961 cggacgatgc tgctcgatcg ccgccagcat cgccgactcg tcgagctcga agttcgcggt
     4021 gagcggcacg ccgacgaagc ttagcccttg cagcctggcc gacatctcgt acatcacgaa
     4081 gcccggcacc ggcgccagga tggttgcgcc tggcacgtcg caggccatgg ccagcaagct
     4141 gatcagctcg tcggaaccat tgcccagcat cagcttgcag ccggcaggca gcttcacgta
     4201 ctgcgccagc gccgcgatca cgtcgcccac gcaggcagtc ggatagcggt tgatcgccac
     4261 acggcccagg cgctcgccca gggcctgctg cagccccacc ggcagccgga aggggttctc
     4321 catcgcgtcg agcttgacca ggccggcact gggctggatc gcgtagccgt gcatggactg
     4381 cacgtcctgg cggatcaccc gctgcatgcg catctgcaaa tcgctcattg cgtgaccccc
     4441 ttcttgttct tggccatccg gaattcggcg gctttcgcgt gcgcctgcaa gccctcgcca
     4501 taggccagct cggccgcgat ctcgcccagc acctgcgcgc cggcttcgct cacctcgatc
     4561 aggctggagc gcttctggaa gtcgtagacg cccagcggcg acgagaagcg cgccgtgccc
     4621 gaagtgggca gtacgtggtt gggcccggca cagtagtcgc ccaggctctc gcttgtgtag
     4681 gcgccgagga agatggcgcc ggcgtgcttg agcagcggct cccaacgttg cggttcgcgc
     4741 gagctcacct ccaggtgctc gggcgcgatg cggttgctga tctcgcaggc ctcttccatg
     4801 ctgcgcgtat ggatcagcgc gccgcggcct tccagcgagg cgcggatcac gtcgcgccgc
     4861 ggcatgccgg gcagcaggcg ctcgatctcg gccttgaccc gcgcgatgta gccggcgtc
//
LOCUS       DASUCY010000104         2554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_516299, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000104 DASUCY010000000
VERSION     DASUCY010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516299"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..608)
                     /locus_tag="VFV25_02070"
     CDS             complement(117..608)
                     /locus_tag="VFV25_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY1392129.1"
                     /translation="MPVTSIELLQRMPIFGAISDEALRFLLEQAGVVQVAAGEFFFRE
                     NDPADSMFVLETGRAAVLKGWRGQELLLHHLEAGDCFGEMALMDLFPRSASVQAMLDC
                     EAIEIRPQHLLRLFEQSPEQFALIQMNIGREVCRRLRATDEQLFRAKMGEAPATVPGA
                     FRS"
     gene            795..1952
                     /locus_tag="VFV25_02075"
     CDS             795..1952
                     /locus_tag="VFV25_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY1392130.1"
                     /translation="MRRWLLLLSLTAMLAGCASAPPKPPAPEGLFRDELFAAPSERIS
                     VDDVFALSDAMRHYLNVDIAAQLRTEGLQQGLIDALRNRGQLRIDYDSTMTRNAAQAF
                     EARSGNCLSLLIMTAAFAKELGLPVTYQSVIIDETWSRSGDIYFASGHVNLMLGKRIT
                     DLRAGYDSSRLLTIDFLAAEDILGQRTKVIDEATVVAMYFNNRAAENLARDRLDDAYA
                     WARAGIRHNPGFVSAYNTLGVIYQRHGNSPEAEVVLRYALEREPRNTQVIANLAQLLG
                     NLGRTEEAQQMQRRLAQLEQDPPFYFFKLGMAAMREGNYKAARDLFALEVERAPDYHE
                     FHFWLGVASYRLGDIAAARKHLALALDDSTTVSDRDLYAAKLAWLKSHQKP"
     gene            complement(1990..>2554)
                     /locus_tag="VFV25_02080"
     CDS             complement(1990..>2554)
                     /locus_tag="VFV25_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY1392131.1"
                     /translation="DLAYQGFGDGIEDDAYAVRALATAGLSFFIANSFSKSMSVYGER
                     CGALSVACPDAKQAENVLGQLKATVRRNYSSPPIHGGQIVAHVLDDPELRALWERELG
                     QMRERILAMRKHLHQVLSAKLPGRNFDYFLSQRGMFSYTGLSAAQVDTLREKHGVYLV
                     RSGRICVAGLNSHNVERTAEAFAAVLG"
BASE COUNT          448 a          877 c          810 g          419 t
ORIGIN      
        1 ctcctttacc tcggatgaca ggaaatgccc gttttagctc accgtgagcc ttctcgggcg
       61 gtcctccata gcatcttggg acggcccggc gcggcgccgg ctagcgccgc ggtccgtcaa
      121 gagcggaagg cacctggcac cgtcgccgga gcctcgccca tcttggcgcg gaacagctgc
      181 tcgtcggtgg cgcgcaaacg gcggcagacc tcccggccga tgttcatctg gatcagcgcg
      241 aactgctcgg ggctttgctc gaagaggcgc agcaggtgct ggggcctgat ctcgatcgcc
      301 tcgcagtcca acatggcctg caccgaggcg ctgcgcggga acaggtccat caaggccatc
      361 tcgccgaagc aatcgcccgc ctccaggtgg tgcagcagca gctcctgccc gcgccagccc
      421 ttgagcaccg cagcgcggcc cgtttccagc acgaacatgg aatcggccgg gtcgttctcg
      481 cgaaagaaga actcgcccgc ggcaacctgc accacgcccg cctgctccag caggaagcgc
      541 agcgcctcgt cgctgatggc gccgaagatc ggcatgcgct gcagcaattc gatgctggtt
      601 accggcatgg catgtctccc gcccccggac ccagagggtg cacacagtga gtgtaggtcg
      661 ccgcagatgc cgacggcgga gcgagcgctt ttcccgaggt gacgggcacg ggctttgcgc
      721 ctacgatgta gacatggctt ggcgtcctgc cagggcacca aacccgctgc gccgatcggc
      781 gcgggagacg tgggatgcgc agatggctgc ttctcttgtc gctgaccgca atgctggcgg
      841 gctgtgcgag cgcgccgcca aagccgccgg cgcccgaggg gctgttccgg gacgagcttt
      901 ttgcggcgcc ttcggaacgc atcagcgtcg atgacgtgtt cgcgctgagc gatgcgatga
      961 ggcactatct gaacgtcgac atcgccgcgc agctgcgcac cgagggcctc cagcaaggcc
     1021 tgatcgacgc gctgcgcaac cgcgggcagc tgcgcatcga ctacgactcc acgatgacgc
     1081 gcaacgccgc ccaggccttc gaagcgcgtt cgggcaattg cctgtcgctg ctcatcatga
     1141 cggccgcctt cgccaaggag ctggggctgc cggtgaccta tcagagcgtc atcatcgacg
     1201 agacctggag ccgcagcggc gatatctact tcgccagcgg tcatgtcaac ctgatgctcg
     1261 gcaagcgcat caccgatctg cgagccggct acgactccag ccggctgctg acgatcgatt
     1321 tcctggccgc ggaagacatc ctcggccagc gcaccaaggt catagacgag gccaccgtcg
     1381 tcgcgatgta cttcaacaac cgcgccgccg aaaacctagc ccgggacagg ctcgatgacg
     1441 cctacgcttg ggcccgcgcc ggaatcagac acaacccggg cttcgtcagc gcctacaaca
     1501 cgctgggcgt gatctatcag cgccatggca attcgcccga agccgaggtc gtgctgcgct
     1561 acgcgctgga acgcgagccg cgcaacaccc aggtcatcgc caacctcgcc cagctgctcg
     1621 gcaatctagg gcgcaccgag gaagcccagc agatgcagcg ccgcctcgcc cagctcgagc
     1681 aggatccgcc cttttacttc ttcaagctgg gcatggcggc catgcgggag ggcaactaca
     1741 aggcggcacg cgatcttttc gcgctagagg tcgagcgtgc gccggactac cacgagttcc
     1801 atttctggct gggcgtggcg agctaccggc tgggcgacat tgccgcggcg cgtaagcatt
     1861 tggctctggc gctagacgac agcacgaccg tcagcgaccg tgatctctac gctgccaagc
     1921 tcgcctggct caagtctcat caaaagccct aggccgccgc ctcgcaggcg gaccgcttcg
     1981 ttccgcacct cagcccaaga ccgccgcaaa ggcctcggcc gtccgctcca cgttgtggct
     2041 gttgagcccg gccacacaga tgcgcccgga gcgaaccagg tagacgccgt gcttctcgcg
     2101 caaggtgtca acctgcgccg cgctcaggcc cgtatagctg aacatgccgc gctggctcag
     2161 gaagtagtcg aagttgcggc ccggcagctt ggcgctcagc acctggtgca ggtgcttgcg
     2221 catcgctagg atgcgctcac gcatttggcc cagctcgcgc tcccagaggg cgcgcagctc
     2281 agggtcgtcc agcacatggg cgacgatctg cccgccgtgg attggcgggc tggaatagtt
     2341 gcggcgcacc gtggccttaa gttggcccaa cacgttctcg gcctgcttcg catccgggca
     2401 ggccacgctg agagcgccgc agcgctcgcc atagacgctc atgctcttgc tgaaggaatt
     2461 ggcgatgaag aaactcaggc ccgccgtggc cagcgcccgc accgcatagg catcgtcttc
     2521 gatgccgtcg ccgaagccct ggtaggcgag gtcc
//
LOCUS       DASUCY010000105         2224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1190935, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000105 DASUCY010000000
VERSION     DASUCY010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190935"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="VFV25_02085"
     CDS             <1..601
                     /locus_tag="VFV25_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY1392132.1"
                     /translation="RVLVLIALAALVWVPIGVWIGMNPRWAGRLQAIAQFLAAFPANL
                     LYPIVVVLLVYWKLNPNIWLSPLMVFGTQWYILFNVIAGASSVPTELRYAAKNLGLTG
                     WLKWKRYLLPAVFPSFVTGAITASGGSWNASIVSEYVTWGDTTLEAQGLGSYIAHMTA
                     AGDFPRIALGIGVMCVFVMSLNRWVWRRLYRLAEDRMHF"
     gene            616..1941
                     /locus_tag="VFV25_02090"
     CDS             616..1941
                     /locus_tag="VFV25_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/sulfonate/bicarbonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HEY1392133.1"
                     /translation="MAQAIIELREVGKSFRSADGTARSVLEGVDFTLREGEIVALLGQ
                     SGSGKSTMLRLMAGLINVDGGNVSYRGQPLHGPARGISMVFQSFALFPWLTVQQNVEL
                     GLEARGAPVEERARRAEKAIELIGLAGFEGALPRELSGGMRQRVGIARALVTEPDVLL
                     MDEAFSALDVLTGERLRDDILELWGSGSMPTRAMLVVSHNIEEAVLMADRVLIFASDP
                     GRIRCQLAVQLPRPRDADSPEVRALIDEVYALMTAGAARAGRPAEERERLNLAERLPD
                     ADVARMEGLLEMLADDDFNGHADLPKLAEETELTDDELLPLANAIAMLGLAQLARGDL
                     NITALGRSYVEASNVGRQEIFGQQLLKNVPLAAHIRHSLEQEPSGELREEPFLRLLRE
                     HLDEAEAERVLRTAIEWGRHGEVFEYNYHTGMIHLPEQSDEEVASTTSG"
     gene            1993..>2224
                     /locus_tag="VFV25_02095"
     CDS             1993..>2224
                     /locus_tag="VFV25_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HEY1392134.1"
                     /translation="MSVNLPALDPSELHPVPGVELGIAMAGVRKANRRDLAVITLAEG
                     SAVAGVFTQNRFCAAPVQLCRKHLAAGAAPRAL"
BASE COUNT          346 a          762 c          758 g          358 t
ORIGIN      
        1 ccgcgtgctg gtgctgatcg cgctcgccgc gctggtctgg gtgccgatcg gcgtgtggat
       61 cggcatgaac ccgcgctggg ccgggcggct gcaggcgatc gcccagttcc tcgccgcctt
      121 cccggccaac ctgctctacc ccatcgtggt ggtgctgctg gtgtactgga agctcaaccc
      181 caacatctgg ctgtcgccgc tgatggtgtt cggcacccag tggtacatcc tgttcaacgt
      241 catcgccggc gcgtccagtg tgccgactga actgcgctat gcggccaaga atttggggct
      301 gaccggctgg ctcaagtgga agcgctatct gctgccggcc gtgttcccga gcttcgtcac
      361 cggcgccatc acggccagcg gcggatcctg gaatgcgagc atcgtgtcgg aatacgtgac
      421 ctggggcgac accacgctgg aggcccaagg cctgggcagc tacatcgcgc acatgacggc
      481 ggcgggcgat ttcccgcgca tcgccttagg gataggcgtg atgtgcgtct tcgtgatgag
      541 cctgaaccgc tgggtctggc gccggttgta tcgtctggct gaagaccgca tgcatttctg
      601 attcgagcac tagccatggc acaagcaatc attgaactgc gcgaagtcgg caagtccttc
      661 cgttcggccg acggcactgc gcgctcggtc ctggaaggcg tggacttcac gctgcgcgaa
      721 ggcgagatcg tggcgctgct gggccagtcc ggctcgggca agagcacgat gctgcggttg
      781 atggcggggc tcatcaacgt ggacggcggt aacgtcagct accgcggaca gcccctgcac
      841 ggcccggcgc gcggcatcag catggtgttc cagtccttcg cgctctttcc ctggctcacc
      901 gtgcagcaga acgtggagct cggcctggaa gcgcgtggcg cgccggtcga ggagcgcgcc
      961 cgccgcgccg agaaggcgat cgagctgatc ggcctggccg gcttcgaggg cgccttgccg
     1021 cgcgaactgt ctggcggcat gcggcagcgc gtgggcatcg cgcgcgcgct ggtcaccgaa
     1081 cccgatgtgc tgctgatgga cgaggccttc tcggccttgg acgtgctgac cggcgagcgc
     1141 ctgcgcgacg acatcctcga gctctggggc agcggcagca tgcccacgcg cgcgatgctg
     1201 gtcgtctcgc acaacatcga ggaagccgtg ctgatggccg accgcgtgct gattttcgcc
     1261 agcgacccgg gccgcatacg ctgccagctc gcggtgcagc tgccgcgccc gcgcgatgcc
     1321 gacagcccgg aggtgcgcgc gctgatcgac gaggtctatg cgctgatgac cgccggagcc
     1381 gcacgcgccg gtcgcccggc cgaggagcgc gagcgcctca acctcgccga gcgcctaccc
     1441 gacgccgacg tggcgcgcat ggagggcctg ctggagatgc tggccgacga cgatttcaac
     1501 ggccacgccg acctgcccaa gctggccgag gaaaccgagc tcaccgacga cgaactgctg
     1561 ccgctggcca atgccatcgc gatgctgggc ctggcccagc tcgcgcgcgg cgacctcaac
     1621 atcacggcgc tggggcgcag ctacgtggag gcgagcaatg tcgggcggca ggagatcttc
     1681 ggtcagcagc tgctcaagaa cgtgccgctg gccgcccaca tccgccacag cctggagcag
     1741 gagccctcgg gcgagctgcg cgaggagcca ttcctgcgcc tgctgcgcga acatctcgac
     1801 gaggccgagg ccgagcgcgt gctgcgcacc gccatcgaat ggggccgcca cggcgaggtc
     1861 ttcgagtaca actaccacac cggcatgatc catctgccgg agcaaagcga cgaggaagtc
     1921 gcgtcgacca cctcgggttg agctgcgccc cttcgtccga gaaattcagc aatcccatag
     1981 agcaccgctg ccatgtccgt caatttgcct gccctcgacc ccagcgaact gcaccccgtg
     2041 cccggcgtgg agctgggcat cgccatggcc ggcgtgcgca aggccaatcg gcgcgacctc
     2101 gccgtcatca cgctggccga gggcagcgcg gtggccggcg tcttcacgca gaaccgcttc
     2161 tgcgccgcgc ccgtgcagct gtgccgcaag catctggccg caggcgctgc cccgcgcgcg
     2221 ctgc
//
LOCUS       DASUCY010000106         3255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_564393, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000106 DASUCY010000000
VERSION     DASUCY010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564393"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VFV25_02100"
     CDS             complement(<1..336)
                     /locus_tag="VFV25_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEY1392135.1"
                     /translation="MSPSTARNKPKVHLLALAAGLACSLQASAQDTAPKAETKAETTL
                     DAVVVTGMKASLEKAQDRKRNADQIVDSIVADDIGKLPDANVAEALQRITGVQISRSR
                     GEGDRVQVRG"
     gene            complement(525..2912)
                     /locus_tag="VFV25_02105"
     CDS             complement(525..2912)
                     /locus_tag="VFV25_02105"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEY1392136.1"
                     /translation="MRWAFMWAGLCLAGSALGQTLLSVDASAPAPAPVSGVLRLGSHV
                     APGGISLGVNNRYLTLQGQPWLPVMGEFHYSRNPAYAWADELRKMKAAGVDIVASYVI
                     WNHHEELPGRFDWSGNRDLRRFVQLAQQAGLKVVVRIGPWAHAEARYGGVPDWVVNAM
                     PTRRDDAEYLHYVERLYGQIGLQLKGLMWKDGGPVIAVQLENEYNLDGPGQGARHIST
                     LKALASKAGLDVPFYTVTGWDGTVYPSGEVTPVFGGYPDEPWGVTTDELRPKETYAFR
                     FDSRVSGDLGAQTAATAPGTAETDADRTPFLGAEFGAGLPAMYRRRTLVSADDIASML
                     PVQLGSGVNLMGYYMFHGGRNPVGRTTLEESTLSGGYNDTPAISYDFNAPLGPDGQQR
                     PVLAKLRPFHSFLHDFGSRLAAMTVRRPDRVSASPADLQTPRWSVRANGDSGFVFFNN
                     HVRQYAMPTFRDVRFSVRLPTGTLVFPSQPITVPHGAYFIWPFNFDMDGTRLNYATAQ
                     PLARLDAGREGIVYVFEASEGIAAEFSIGQQVLTMEPGAAPREVGHAAGRPLRILVLR
                     PDQAQRLVIGELAGRRRLILTQAQVWFDASGLQLRSVGESRQHVAIFPSLERPAAHGA
                     ALRLAGTEGAFQALDLEWPARTPLASFSVLREAGTAPPVMKGGLAGAALQPIPEAFKA
                     AAAWRITLPRDALEGVDDTLLELDFVGDIARLFAGTRMLDDWYYNGQRWEVGLKGLKH
                     LGQLRSEPLTLTVMPLRADAPIYIDRSHRPDFAGQPQIAQLRSVTLKPIYRATLK"
     gene            complement(2973..>3255)
                     /locus_tag="VFV25_02110"
     CDS             complement(2973..>3255)
                     /locus_tag="VFV25_02110"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167831.1"
                     /note="catalyzes the formation of D-glyceraldehyde
                     3-phosphate and pyruvate from
                     2-dehydro-3-deoxy-D-galactonate 6-phosphate; functions in
                     galactonate metabolism; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HEY1392137.1"
                     /translation="PTEAFAAVRAGADALKLFPAELITPPVVRAMRSVLPRELRLLPV
                     GGITPQSMSEFRRAGAAGFGLGSALYAPGLDAAAVAGRGRAFVEAWRAF"
BASE COUNT          503 a         1094 c         1110 g          548 t
ORIGIN      
        1 gccgcgcacc tgcacccggt cgccctcacc gcggctgcgc gagatctgca cgccggtgat
       61 gcgctgcagc gcctcggcca cattggcatc gggcagcttg ccaatgtcgt cggccacgat
      121 ggaatcgacg atctgatcgg cattgcgctt gcggtcttgg gccttctcca ggctggcctt
      181 catgccggtg acgacgacgg cgtccagtgt cgtttcggcc tttgtctcgg ctttcggggc
      241 ggtgtcctgt gccgaggcct gcagcgagca ggccaggccg gcggccagcg caagaagatg
      301 gaccttgggc ttgtttcgag cggtgctcgg gctcatgcac ttgtctcctg gttatttgtt
      361 gaatatgtcg agcgacccga aagttaccga gagcccgctt cacgcactaa tgaaagattt
      421 cgtttttgct atctcggctt tgcatatcgc gcgtcatgcg acgcatgaaa cgaggctccc
      481 cctctcccgc gggcgggaga gggttggggt gaggggctcc ggtttcactt cagcgtggcg
      541 cgatagatgg gcttcagcgt cacgctgcgc agctgcgcaa tctgcggttg gcccgcgaaa
      601 tcggggcggt gcgatcgatc gatgtagatc ggcgcatcgg cgcgcagcgg catcacggtg
      661 agcgtgagcg gctcgctcct cagttgcccc aggtgcttca agccctttag gcctacctcc
      721 cagcgctggc cgttgtagta ccagtcgtcc agcattcgcg tgccggcgaa gaggcgagcg
      781 atgtcgccca cgaagtccag ctccagcagg gtgtcgtcca caccttcgag cgcgtcgcgc
      841 ggcagcgtga tgcgccacgc agcggccgcc ttgaaggctt cgggtatggg ctgcagggcc
      901 gcaccggcca ggccgccctt catcacgggg ggcgcggtcc cggcttcacg caagacgctg
      961 aagctggcta gcggcgttcg ggccggccat tccaggtcca gcgcctggaa ggctccttcg
     1021 gtaccggcga gccgcagtgc cgcaccatgc gcagcgggtc gctccaggga ggggaagatc
     1081 gccacgtgct gccggctctc gcctaccgag cgcagctgaa gaccgctcgc atcgaaccag
     1141 acctgggcct gcgtgaggat caagcggcgc cggccggcca gttcgccgat gacgagccgc
     1201 tgcgcctgat ccggccgcag aaccaggatg cgcagcggcc ggcctgccgc gtggccgacc
     1261 tcgcgcggcg ccgcccccgg ctccatcgtc agcacctgct ggccgatgct gaattccgcc
     1321 gcgatgccct cgctggcctc gaacacatag acgatgccct cgcgcccggc gtccaggcgg
     1381 gccagcggct gggcggtggc gtagttcagc cgcgtgccgt ccatgtcgaa attgaagggc
     1441 cagatgaaat aggccccgtg gggaaccgtg atcggttgac tcgggaacac cagcgttcct
     1501 gtgggtagcc ggaccgagaa gcgcacatcc ctgaacgtcg gcatcgcgta ttgccggacg
     1561 tggttgttga agaacacgaa gccgctgtcg ccgttcgcgc gcacggacca gcgcggcgtt
     1621 tgcagatcgg ccggcgacgc gctcacgcgg tccggccggc gcacggtcat ggcggcgagc
     1681 cgcgagccga agtcgtgcag gaaagagtgg aaaggccgca gctttgccag caccggccgc
     1741 tgctggccgt cgggccccag gggtgcgttg aagtcgtagc tgatggcggg cgtgtcgttg
     1801 tagccgccgc tcagcgtgct ctcttccagc gtcgtgcggc ccaccgggtt gcgcccgccg
     1861 tggaacatgt agtagcccat cagattgacg cccgagccca gctgcaccgg cagcatggag
     1921 gcgatatcgt ctgccgagac cagtgtgcgg cgccgataca tcgccggcag cccggcgccg
     1981 aattcagcgc cgagaaatgg cgtcctgtcg gcatcggtct ccgcggtccc cggggcggtg
     2041 gcggcggtct gcgctccgag gtcgccgctg acacggctgt cgaagcggaa ggcataggtt
     2101 tccttaggtc gcagctcatc tgtcgtcacg ccccagggct cgtcgggata gccgccgaag
     2161 acgggggtga cctcgcccga gggatagacc gtgccgtccc agccggtcac cgtgtagaaa
     2221 gggacgtcca gcccggcctt gctggccagg gccttgagcg tgctgatgtg cctggcgccc
     2281 tggccggggc cgtcaaggtt gtattcgttc tccagctgca cggcgatcac cggcccgccg
     2341 tccttccaca tcaggccctt gagctgcagg ccgatctgcc catacaagcg ctctacgtag
     2401 tgcagatact cagcgtcatc gcggcgggtc ggcatggcat tcacgaccca gtcgggcacg
     2461 ccgccgtagc gcgcctcggc atgggcccag gggccgattc gcaccaccac cttgaggccg
     2521 gcctgctgcg ccagttgcac gaagcggcgc aggtcgcggt tgccgctcca gtcgaagcgg
     2581 cccggcagct cctcgtgatg attccagatc acgtagctgg cgacgatgtc cacgccggcg
     2641 gccttcatct tgcgcagctc gtcggcccag gcataggccg ggttgcgcga gtaatgaaac
     2701 tcgcccatca cgggcagcca gggctggcct tgcagcgtga gatagcggtt gttcacgccc
     2761 agtgaaatgc cacccggcgc gacgtggctg cccagacgca agacgccgct cacgggcgcg
     2821 ggcgcggggg cactggcgtc cacgctcagc agggtctgcc ccagcgccga tccggccagg
     2881 cacagaccgg cccacataaa ggcccatcgc atcgctgtct ccttcttctc agggtcaaca
     2941 gaccctggtc ttttgtgcag gcttctgtcg ggttagaacg ctcgccaagc ctcgacgaat
     3001 gcacgcccgc ggccggccac cgctgcggcg tccaggcctg gggcatacaa ggcggatccg
     3061 aggccaaagc cggccgcgcc ggcgcgacgg aattcgctca tggattgcgg cgtgatgcct
     3121 cccacgggca gcagcctgag ttcccgcggc agcaccgacc gcatcgcgcg cacgaccggc
     3181 ggcgtgatca gctcggcggg gaagagcttc agcgcgtcag ccccggcgcg cacggccgcg
     3241 aacgcttcgg tcggc
//
LOCUS       DASUCY010000107         6495 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_516339, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000107 DASUCY010000000
VERSION     DASUCY010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 6495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6495
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516339"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VFV25_02115"
     CDS             complement(<1..260)
                     /locus_tag="VFV25_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392138.1"
                     /translation="MKTRFLLPLAVAAAALAGCATTPYSELYGYRYYVTNIDTYSVIV
                     SKVDGKSTPLRMPALVDPGLHKIVVQGPPTVTNRYGEEREISL"
     gene            complement(291..1223)
                     /locus_tag="VFV25_02120"
     CDS             complement(291..1223)
                     /locus_tag="VFV25_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY1392139.1"
                     /translation="MNSPQQAAPSALQRFFDGDVWHSFRNSPVAMLAALIACVCIFCA
                     LFANWVAPHNPFDLATLELGDARLPPAWMEGGTGKYLLGTDDQGRDILSALMFGARIS
                     LFVGLASVLLSMLVGVLLGLLSGFAGGHIDALIMRVCDVMLSFPAILIALLIDGVGRA
                     LFPGAHESLAFAVLILAIAITGWVQYARTVRGSTLIERNKEYVQAARVIGVAPLRIML
                     RHVLPNVLGPVLVLATIQVAQAIIIEATLSFLGVGVPPTSPSLGTLIRVGNDFLFSGE
                     WWITIFPGAMLVLIALSVNLLGDWLRDALNPRLR"
     gene            complement(1297..2868)
                     /locus_tag="VFV25_02125"
     CDS             complement(1297..2868)
                     /locus_tag="VFV25_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY1392140.1"
                     /translation="MKHKKTLVLAALALACAWAQGLTLRVADVGDVQSMDPHSLNETL
                     QLSFDGNVYESLIGRGKDMSLVPLLATKWTPTSPTVWRFELRHGVKFHDGTPFTADDV
                     VFSFKRGASDGSDMKSYMSPIKEVRKINDYVVDVETAAPFPILPETLTVFYMMSKKWC
                     EENKAERPVDRRKGVENTASFKANGTGPYRVKERQPSTRTVLVRNFNYWGKVDGNVDE
                     IVFTPIGNDATRVAAMLSGEIDLMEPVPLQDVDRIKAAGFTVMQGPELRTIFLGMDQR
                     RDELLFSNIKGKNPFKDKRVRQAFYQAIDIEAIKSRVMRNAATPSAEMVGPGVRGFQL
                     DMNKRLPYDVEAAKKLMAEAGYPAGFEVGMNCPNDRYVNDAQICQAVAANLARIGVKV
                     NLQAETKNSYFPKILRRDTSFYLLGWTPSTTDAHDAMTSLMATPNDKGQGQFNLGWYS
                     NPKFDELTHQIQSETDAAKRNAMIREAFKIHQDDVGHIPLHQQALAWAMKRGISAVQL
                     PTNYMFFKWVNVKVD"
     gene            complement(2911..4164)
                     /locus_tag="VFV25_02130"
     CDS             complement(2911..4164)
                     /locus_tag="VFV25_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HEY1392141.1"
                     /translation="MLQNVKHGTYQRLAGWHHELTAFRRDLHAHPELGFEEVRTAGRV
                     AEALRLFGCDEVHTGIGKTGVVALVRGARPVGNGAQSPQQAMGDGQKVRAIGLRADMD
                     ALPMCEENDFAWRSAKNGLMHGCGHDGHTTMLLGAARYLAQTRDFAGTAVLIFQPGEE
                     GYAGAKAMIEDGLFERFPVDAVYAMHNWPNLPAGTFGVNPGPMMAAADRVEITINGKG
                     GHGAHPYMAVDPVLVAAHIITAAQSLVSRNVNPLDSAVVSLCAVQAGDLGAFSVIPRQ
                     ARLMGTVRSFRHDVQDMLEERLGRLVESVAVGLGATAVLDYHRVYPATINTPDEANFA
                     ADIATSLVGPQRVVRDLPPSMGAEDFSFMLQNRPGAYLRIGQGSDDSSCFLHNSRYDF
                     NDEILPLGSALFASLVEAALPSATS"
     gene            complement(4166..5149)
                     /locus_tag="VFV25_02135"
     CDS             complement(4166..5149)
                     /locus_tag="VFV25_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY1392142.1"
                     /translation="MLAFILRRLAQAAIVMLTVAFIAFMLFQYVGDPVINLLGQDATP
                     QQRAQLRADLGLDQPFPVQFAHFVVNAAQGEFGLSLRQGRKVSSLIVERLPATLELSF
                     VAALLALLVGVPLGVYAALRRGSFLSQLAMTLSLLGVSLPTFLIGILLILVFSVLLKW
                     LPSFGRGAVVQLGAWSTGFLSADGWRHLILPGVTLAIFQLTLIMRLVRAEMLEVLRTD
                     YIKFARARGLSDRAVHFGHALKNTLVPVITITGLQLGGLIAFAIITETVFQWPGMGLL
                     FIQAVTFADIPVMAAYLCLIALIFVLINLVVDLLYFAVDPRLRIERAAGGH"
     gene            complement(5399..6439)
                     /locus_tag="VFV25_02140"
     CDS             complement(5399..6439)
                     /locus_tag="VFV25_02140"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY1392143.1"
                     /translation="MKKSLLALAVLSAFAGAASAQSSVTLYGRVDVALGKAPFDNDKQ
                     EANGSGSRVGLRGVEDLGGGLAAVFDIQHRFNAQNGAQTATRFWHGLSVVGLKGDFGQ
                     VLLGRNYTASYTYGQVAADPWGGDTVVSGNYMKTTRTPAAPTGFTSDRGTNAILLGGI
                     GATRNDQSITYSFSQSGFTFAAQLAESDPAIAPGFGSSSNNAPFANKPFNFALAYNAG
                     PIAAGVSHERPGADGAKWSTGYFSYNLDVVKLGAFFGSGTNGNNAGYMKGGNVRSWMV
                     TATAPLGGGEARAAYGQRKEGDVRDVRLGAIGYHYPLSKRTKLYTDAIKNGGAGTPTD
                     KWGYDFGLQHNF"
BASE COUNT         1208 a         2051 c         2174 g         1062 t
ORIGIN      
        1 agcgagatct cgcgctcctc gccgtagcgg ttggtcaccg tgggcggccc ctgcacgacg
       61 atcttgtgca gcccgggatc gaccagcgcc ggcatgcgca gcggcgtgct cttgccgtcg
      121 accttggaga cgatcaccga ataggtgtcg atgttggtga cgtaatagcg ataaccgtag
      181 agctcggaat aaggcgtcgt ggcgcagccg gcaagagcag cggcggcgac ggccagaggg
      241 agcagaaaac gagtcttcat gaccagctct cctttcttcg cgtgaatgcc tcagcgaagc
      301 cttgggttca gcgcatcgcg cagccagtcg cccagcaggt tcacgctcag cgcgatgagc
      361 accagcatag cgccggggaa gatcgtgatc caccactccc cactgaaaag aaagtcattg
      421 ccgacgcgaa tcaatgtacc gagcgaaggc gaggtcggcg gcacgcccac gcccaggaag
      481 gacagcgtgg cctcgatgat gatggcctgg gccacctgga tggtcgccag caccagcacc
      541 gggcccagca cattgggcag cacatgcctg agcatgatgc gcagcggggc gacgccgatc
      601 acgcgggctg cctgcacata ctccttgttg cgctcgatca gcgtcgagcc gcgcacggtg
      661 cgcgcgtatt gcacccagcc ggtgatggcg atggccagga tcagcaccgc gaaggccagc
      721 gattcatggg cgccgggaaa cagcgcccgc ccgacgccgt cgatcagcag cgcgatcagg
      781 atggccggga aggacagcat cacgtcgcac acgcgcatga tgagtgcgtc gatgtgcccg
      841 ccggcgaagc ccgacagcag ccccagcagc acgcccacca gcatcgacag cagcacggag
      901 gccagaccga cgaagagcga gatgcgtgcg ccgaacatca gcgccgaaag aatatcgcgc
      961 ccctgatcgt cggtgcccag aaggtatttg ccggtgccgc cctccatcca ggccggcggc
     1021 aggcgggcgt cgcccagctc cagcgtcgcg agatcgaagg ggttgtgcgg cgcgacccaa
     1081 ttggcaaaca gggcgcagaa gatgcagacg caggcgatca acgccgccag catggcgacc
     1141 ggcgagttcc ggaagctgtg ccagacatcg ccatcgaaga atcgctgcag tgcgctgggc
     1201 gcggcttgct gcggcgagtt catgccggcc ggaccgagca gggcacccgc gtcccgctcg
     1261 ctgggcgagt cgagaggcac gccggcggac gaagcttcag tcgactttga cattgaccca
     1321 cttgaagaac atgtagttgg tcggcagctg aacggcgctg ataccgcgct tcatcgccca
     1381 ggccagggcc tgctggtgca gcggaatgtg gccgacgtcg tcctggtgga tcttgaaggc
     1441 ctcgcggatc atcgcgttgc gcttggcagc atcggtctcg gactggatct ggtgggtcag
     1501 ctcgtcgaac ttcgggttgc tgtaccagcc gaggttgaac tggccctggc ccttgtcgtt
     1561 gggtgtggcc atcaacgagg tcatcgcgtc gtgggcgtct gttgtgctgg gcgtccagcc
     1621 gagcaggtag aagctggtat cgcggcgcag gatcttgggg aaatagctgt tcttggtttc
     1681 cgcctgcaga ttgaccttga cgccgatgcg tgccaggttg gcggccaccg cctgacagat
     1741 ctgcgcgtcg ttcacgtagc ggtcgttggg gcagttcatg cccacctcga agccggcggg
     1801 gtagccggcc tcggccatca gcttcttggc cgcctccacg tcatagggca ggcgcttgtt
     1861 catgtcgagc tggaaaccgc gcacgccggg gcccaccatt tcggcgctcg gcgtcgcggc
     1921 attgcgcatc acgcgcgact tgatggcctc gatgtcgatc gcctggtaga aggcctggcg
     1981 cacccgcttg tccttgaagg ggttcttgcc cttgatgttg ctgaacagca gctcatcgcg
     2041 cctctgatcc atgcccagga agatggtgcg cagctcaggc ccttgcatca cggtgaagcc
     2101 ggcggccttg atgcggtcca cgtcctgcag cggcaccggc tccatcaggt cgatctcgcc
     2161 ggacagcatc gccgccacgc gcgtagcgtc gttgccgata ggcgtgaaga cgatctcgtc
     2221 gacgttgccg tcgaccttgc cccagtagtt gaagttgcgg accagcacgg tacgcgtgga
     2281 gggctggcgt tccttcacgc ggtaagggcc ggtgccgttg gccttgaagg aggccgtgtt
     2341 ctcgactccc ttgcggcgat ccaccgggcg ctcggccttg ttttcctcac accacttctt
     2401 gctcatcatg tagaacacgg tgagtgtttc cggaaggatg gggaaaggcg cggcggtctc
     2461 gacatccacc acgtagtcgt tgatcttgcg tacctccttg atcggcgaca tgtagctctt
     2521 catatcagag ccgtcggagg caccgcgctt gaaggagaac accacgtcgt cggccgtgaa
     2581 gggcgtgccg tcatggaatt tgacgccgtg gcgcagctcg aagcgccaca cggttggcga
     2641 ggtcggcgtc catttggtgg ccaagagcgg caccaggctc atgtccttgc cgcggccgat
     2701 caggctctcg tagacgttgc catcgaagct cagctgcaga gtctcgttga gcgaatgcgg
     2761 gtccatggac tgcacgtcgc ccacatcggc cacgcgcagc gtgaggcctt gcgcccaggc
     2821 gcaggcaaga gccaaagcag cgaggaccag cgtcttcttg tgcttcatcg cgatctcctt
     2881 gggaaacgtg agaggaagga aggcgaaccg tcagctcgtg gccgacggca gggccgcctc
     2941 gaccagcgag gcgaagaggg ccgaacccag cggcagaatc tcgtcgttga aatcgtagcg
     3001 gctgttgtgc aggaagcagc tgctgtcgtc cgagccctgg ccgatgcgca gataggcgcc
     3061 cggacggttc tgcagcatga aggagaagtc ctcggcgccc atgctgggcg gcaggtcgcg
     3121 caccacacgc tgcgggccga ccaggctggt cgcgatatcg gccgcgaagt tcgcctcatc
     3181 cggggtgttg atggtggccg gatagacgcg gtgatagtcg agcacggccg tcgcccccag
     3241 gccaaccgcg accgactcga ccagtcggcc caggcgctcc tcgagcatgt cctgcacgtc
     3301 gtggcggaag ctgcgcaccg tgcccatcag cctggcctgg cgcggaatga cgctgaaggc
     3361 cccaaggtcg ccggcctgca cggcgcacag gctcaccacc gcactgtcca agggattgac
     3421 attgcgcgac accaggcttt gcgccgccgt gatgatgtgg gccgcgacca gcaccggatc
     3481 gaccgccatg tagggatgcg cgccatggcc gcccttgccg ttgatcgtga tctcgacgcg
     3541 atcggccgcg gccatcatcg ggcctggatt cacgccgaaa gtgcctgccg gcagattggg
     3601 ccagttgtgc atcgcgtaga ccgcatcgac cgggaagcgc tcgaacaggc catcctcgat
     3661 cattgccttg gcgccggcat agccctcctc gccgggctgg aagatcagca ccgcggtgcc
     3721 ggcgaagtcg cgcgtctggg ccagatagcg cgccgcgccg agcagcatgg tggtgtgccc
     3781 gtcgtggccg cagccatgca tcaggccgtt cttggccgag cgccacgcga aatcgttctc
     3841 ctcgcacatc ggcagggcgt ccatgtcggc ccgcaggccg atggcacgga ccttctggcc
     3901 atcgcccatg gcctgctgcg gcgattgcgc gccgttacca acgggcctgg cgccgcgcac
     3961 tagggcgacc acgccggtct tgccgatgcc ggtgtgcacc tcgtcgcagc cgaagagccg
     4021 tagcgcctcg gccacgcggc ctgcagtgcg cacctcctcg aagccgagtt cggggtgggc
     4081 gtgcaggtcg cggcggaagg cagtcagctc atgatgccag ccggccaggc gctggtaggt
     4141 gccatgcttg acgttttgca gcatctcaat gccctcctgc ggcgcgctcg atgcgcagcc
     4201 gcgggtcgac cgcgaagtac agcaggtcta ccacaaggtt gatcagcacg aagatcaggg
     4261 cgatcaggca cagataggcc gccatcaccg ggatgtcggc aaaggtgacg gcctggatga
     4321 agagcagccc catgcccggc cattggaaca ccgtctcggt gatgatggca aaggcgatca
     4381 ggccgccgag ctgcaggccg gtgatggtga tcacaggcac cagagtgttc ttcagtgcat
     4441 ggccgaaatg cacggcccgg tccgaaaggc cgcgggcccg cgcgaacttg atgtagtcgg
     4501 tgcgcagcac ctcgagcatc tcggcgcgca ccagccgcat gatcagcgtg agctgaaaga
     4561 tggccagcgt cacgccgggc aggatcaagt gcctccagcc gtccgcgctc agaaagcccg
     4621 tactccaggc cccgagctgc acgaccgcgc cgcggccgaa gctcggcagc catttcagga
     4681 gcaccgagaa caccaggatc agcaggatgc cgatcaggaa ggtgggcagt gagacgccca
     4741 gcagcgagag cgtcatcgcc agctgcgaaa gaaagctccc gcgcctgagc gccgcataga
     4801 cccccagggg cacgcccacc agcaaggcca gcagcgccgc aacgaaggac agctccagcg
     4861 tcgccggcag gcgctcgacg atcagcgaag agaccttgcg cccctggcgc aggctcaggc
     4921 cgaactcgcc ctgcgccgcg ttgacaacga aatgagcgaa ctgcaccggg aagggctggt
     4981 ccagccccag atcggcccgc agctgggcgc gctgctgggg cgtcgcgtcc tggccaagca
     5041 gattgatcac cggatcgccg acatactgaa acagcatgaa ggcgatgaag gccaccgtga
     5101 gcatcacgat ggcggcttga gcgaggcggc gaagtatgaa ggcgagcatg ccggggcttg
     5161 gggaccgatc acggattgcc tgcaggcgaa gcgtcgtcag tcacgacgcg tccggcagca
     5221 atgtccttat ggggtgatgc aaataacgga ccggaacgaa ccgacgtcga tgatccgtat
     5281 gcaggggaac cctcgtcaaa ggggattgcc cttagcgttt gacgcgagag cgatgaaaaa
     5341 ggccgcccga aggcggcctt gagcttctga ggacacccgc atcgctgcgg gctctcgatc
     5401 agaagttgtg ctgcagaccg aagtcgtagc cccacttgtc ggtcggcgtg ccggcgccgc
     5461 cgttcttgat cgcatcggtg tacagcttgg tgcgcttgct cagcggatag tggtagccga
     5521 tggcgcccag gcgaacgtcg cgaacgtcgc cttccttgcg ctggccgtag gccgcgcggg
     5581 cctcgccgcc gcccagcgga gcagtggcag tcaccatcca cgagcggaca ttgccgccct
     5641 tcatgtagcc ggcgttgttg ccgttggtgc cggaaccgaa gaaggcaccc agcttcacca
     5701 catcgaggtt gtaggagaaa tagcccgtgc tccactttgc accgtccgcg cccggacgct
     5761 catgcgacac gccagcggcg atcgggccgg cgttgtaggc cagcgcgaag ttgaacggct
     5821 tgttggcaaa cggagcgtta ttcgacgagc tgccaaagcc gggggcgatg gcgggatccg
     5881 actcggccag ctgagcagcg aaggtgaagc cggactgcga gaagctgtag gtgatcgact
     5941 ggtcgttgcg agtcgcgccg atgccgccca gcaggatggc gttggtgcca cggtcagaag
     6001 taaagccagt gggggcggcg ggcgtccggg tcgtcttcat gtagttgccc gaaacgacgg
     6061 tgtcaccacc ccagggatcg gcagccactt ggccgtaggt gtacgaggcg gtgtagttgc
     6121 ggcccagcag cacttggccg aagtcgccct tcaggccgac cacggacaga ccgtgccaga
     6181 agcgggtcgc ggtctgcgcg ccgttctggg cgttgaagcg gtgctggata tcgaacacgg
     6241 cagccagacc gccgccgagg tcttccacgc cacgcaggcc gacgcggctg ccggagccgt
     6301 tggcctcctg cttgtcgttg tcaaagggcg ccttgcccag agccacgtca acacgaccat
     6361 acagagtgac cgacgactga gccgaagcag cacccgcgaa agcgctcagg actgcgagag
     6421 cgagcagaga ttttttcatt gctaactatc tccaaggttg aaacaagagg tcccaaaaaa
     6481 gcccctgcgc gcacg
//
LOCUS       DASUCY010000108         2742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_34308, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000108 DASUCY010000000
VERSION     DASUCY010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34308"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1990
                     /locus_tag="VFV25_02145"
     CDS             <1..1990
                     /locus_tag="VFV25_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY1392144.1"
                     /translation="EAARICGIDEAALVQAARWFGESPATLSLYCQGLNQSTSGTDKN
                     AALINLHLATGQIGKPGAGPFSLTGQPNAMGGREVGGLANLLSAHRDLGNAGHRAEVA
                     ALWGVKDVPAAPGKTAVEMFEAAARGEIKALWIVCTNPAQSLPEQATVRQALQTAEFV
                     VVQEAYATTATCAYADLLLPATTWGEKEGTVTNSERRISRVRRAVAAPGEARDDWRIA
                     VDFAHSLEVRLSDRPNGQATLFPYAAPEAVWNEHRASTRGRDLDITGLSYALLDAQGP
                     QQWPLPEGAVQGRARLYEDGVFPTDDGRARFAAVKYRPVAEPRDARYPFSLTTGRLRD
                     QWHGMSRTGVLGRSFGHVPEPCIELHPQELTRRGWAEGELLHVTSRRGSLVLPVQASE
                     AVAPTQAFIAMHWGEEFISGSTASGARVTGVNALTTPAFCPKSKQPELKHAAVKLLKA
                     ELPWRLLAMAWLPQDRALHAQQALRELMPSFSYASCVPFGREPHPRGLAGVLFRAAAY
                     EAAPEALIAQIEAALGLAGADVLNYADKRRGQRRAMRLARDGSGARLEGFLLAGDTSA
                     EAWIRTLLQDELPAQAYGRLLLVPGARAPVAVKPKGRQVCTCFNVAEPEIIATLQACA
                     GTAEARLAQLQDRLKCGTNCGSCLPELKKLERQQQQVA"
     gene            2038..>2742
                     /gene="ybiB"
                     /locus_tag="VFV25_02150"
     CDS             2038..>2742
                     /gene="ybiB"
                     /locus_tag="VFV25_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HEY1392145.1"
                     /translation="MSIASYIKVIGRGKEGARALNQAQAHDLMSQVLDGRVSDLEIGA
                     FALAMRIKGETGDELAGFLQATEERCLHLAPPRPAVLLASYNGARRLPNLTPLLALLL
                     ARAGLAVLVHGPLQDPGRVTSAMIFEALGLPPAGSAADVQAAWDRREPAFIAIETLSP
                     QLAKLLAVRWTVGLRNSGHTIAKLLDPCSSEDGPPALRVISYTHPEYGKGHADFLAAT
                     RANAMLLRGTEGEPVAD"
BASE COUNT          439 a          996 c          936 g          371 t
ORIGIN      
        1 ggaggcggcg cgcatctgcg gcatcgacga ggcggcactg gtgcaggccg cgcgatggtt
       61 cggtgaatcg cccgcgacgc tgtcgctcta ttgccagggc ctgaaccaga gcacgagtgg
      121 caccgacaag aacgccgcac tgatcaacct gcaccttgcc accggccaga tcggcaagcc
      181 tggcgcgggg cctttctcac tcaccggcca gcccaatgcg atgggcgggc gcgaagtggg
      241 cgggctggcc aacctgctga gcgcgcatcg cgatctcggc aatgccgggc atcgcgccga
      301 ggtcgccgcg ctgtggggcg tgaaggacgt gcccgcggca cccggcaaga cggcggtgga
      361 gatgttcgaa gctgcggccc gcggcgagat caaggctctc tggatagtct gcaccaaccc
      421 ggcccagtcg ctacccgagc aggccactgt gcgccaggcc ctgcagaccg cggagttcgt
      481 cgtcgtgcag gaggcctatg cgaccactgc cacctgtgcc tatgccgacc tgctgctgcc
      541 cgccaccacc tggggcgaga aggaaggcac agtgacgaac agcgagcggc gtatcagccg
      601 cgtgcgccgc gccgtggctg cgccgggcga ggcgcgcgac gactggcgca tcgcagtcga
      661 cttcgcgcac agcctggagg tgcggctgtc ggaccggccg aacgggcaag ccacgctctt
      721 cccctacgct gcacccgagg ccgtttggaa tgaacatcgc gcatcgacgc gtggccgaga
      781 tctcgacatc acgggtctga gctatgcact gctggatgcg caaggcccac agcaatggcc
      841 cctgcccgag ggcgcagtgc aaggccgagc tcgcctctac gaagacggtg tgttccccac
      901 cgatgacggc cgagcccgct tcgccgcggt gaagtaccgc ccagtggccg agccgcgcga
      961 tgcgcgctac cccttctcgc tcaccacggg gcgactgcgt gaccagtggc atggcatgag
     1021 ccgcaccggc gtgctgggcc gctccttcgg ccacgtgccc gagccctgca tcgagctgca
     1081 tccgcaggag ctgacgcgcc gcggctgggc cgaaggcgag ctgctgcacg tgacctcgcg
     1141 gcgcggctcg ctggtgctgc cggtgcaggc gagcgaggcg gtggcgccga cccaggcctt
     1201 cattgcaatg cactggggcg aggagttcat ctccggcagc acggccagcg gcgcacgcgt
     1261 gaccggcgtg aatgcgctga cgacgccggc cttctgcccc aagtcgaagc agcccgaact
     1321 caagcacgcg gccgtcaagc tgctgaaggc cgagctgccc tggcgcctgc tcgcgatggc
     1381 ctggctgccg caggaccgtg ccctgcatgc gcagcaggcc ctgcgcgagc tgatgccctc
     1441 cttctcctat gcaagctgcg tccccttcgg ccgcgagccg catccgcgcg gcctcgccgg
     1501 tgtgctcttc cgcgccgccg cctacgaagc cgcgcccgag gcgctgatcg cgcagatcga
     1561 agccgcgctg ggcctggccg gcgccgacgt gctgaactac gccgacaagc gccgcggcca
     1621 gcgccgcgcg atgcgcctgg cccgagacgg cagcggcgcg cggctcgaag gcttcctgct
     1681 ggccggcgac actagcgccg aggcctggat caggacgctg ctgcaggacg agctgcccgc
     1741 ccaggcctat ggccgcctgc tgctcgtgcc gggcgccagg gcgccggtgg cggtcaagcc
     1801 caaaggccgc caagtctgca cctgcttcaa cgtcgccgag cccgagatca tcgccacgct
     1861 gcaagcctgc gccggcacgg ctgaagccag gctcgcgcag ctgcaggacc gcctcaaatg
     1921 cggcaccaac tgcggctcct gtctgcctga gctgaaaaaa ctcgagcggc agcaacagca
     1981 ggtggcttga gggcgacggc gacgcgccgg cctcaaaatc gaggccagga agcacgcatg
     2041 agcatagcca gctatatcaa agtcatcggc cgcggcaagg aaggcgcgcg ggcactgaac
     2101 caggcccagg cccatgacct gatgagccag gtgctggacg gccgcgtcag cgatctggag
     2161 atcggcgcct tcgcgctggc tatgcgcatc aagggcgaga ccggcgacga gctggccggt
     2221 ttcctgcagg ctaccgaaga acgctgtctg cacctcgccc caccacgccc tgccgtgctg
     2281 ctcgcctcct acaacggcgc acggcggctg cccaatctga caccgctgct cgcgctgctg
     2341 ctggcgcgcg cgggccttgc cgtgctggtg catgggccgc tgcaggaccc cggtcgcgtg
     2401 accagcgcca tgatcttcga agccttgggc ctgccgccgg ccggcagcgc ggccgacgtg
     2461 caggccgcct gggaccgccg cgagcccgcc ttcatcgcca tcgagacact cagcccccag
     2521 ctggccaagc tgctggcggt gcgctggacc gtgggcctgc gcaactccgg ccacaccatc
     2581 gccaagctgc tcgacccctg cagcagcgag gacggcccgc ccgcgctgcg cgtgatcagc
     2641 tacacccatc ccgaatacgg caagggccac gcggacttcc tcgccgcgac gcgggccaat
     2701 gcgatgctgc tgcgcggcac cgaaggcgag ccggtggccg ac
//
LOCUS       DASUCY010000109         4039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_757231, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000109 DASUCY010000000
VERSION     DASUCY010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757231"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /gene="waaF"
                     /locus_tag="VFV25_02155"
     CDS             complement(<1..740)
                     /gene="waaF"
                     /locus_tag="VFV25_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEY1392146.1"
                     /translation="MTRSLVIAPQWIGDAVMAEPLLAALAVRGEVLTVAALPWVAPVL
                     RAMPQVSEVIELPFAHGRLDWAARKAMAATLRGRFERAYVLPNSIKSALLPWLARIPR
                     RLGYQGEGRWLLLNERLSNPPRGQERPPMATYYGALAGRTATKLKPQLSLPPAQVEVV
                     LQRVGVSRRAYWVFAPGAEYGPAKRWPSENYAALARQLFAQSRQPVLLLGSAKEGEIC
                     DEISLLAPGACHVMAGRTTLDEAMALIAG"
     gene            complement(862..1086)
                     /locus_tag="VFV25_02160"
     CDS             complement(862..1086)
                     /locus_tag="VFV25_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEY1392147.1"
                     /translation="MSTENKSPATAVATVEVTAKDLQGPGVVFCPNPKMPLWSNHPRV
                     YIDVAAEGEGKCPYCGTVYKLKAGEKLHTH"
     gene            complement(1101..2027)
                     /locus_tag="VFV25_02165"
     CDS             complement(1101..2027)
                     /locus_tag="VFV25_02165"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEY1392148.1"
                     /translation="MGLDDRDGKIWMDGELVDWRDAKVHVLTHTLHYGCGAFEGVRAY
                     KTAAGTTAIFRLREHTERLFNSAKILRMKIPYSFEQVMEAQRQVVRANKLDSCYLRPL
                     TWIGSEKLGVSPKGNTIHLMVAAWAWGAYLGEEGMRRGIRVKTSSYTRHHVNITMTQA
                     KTVSNYTNSILANMEATEDGYDEAMLLDASGFVSEGAGENLFIVKNGVLYTPDLSAGA
                     LNGITRNTVFAICADLGLKLVEKRITRDEVYIADEAFFTGTAAEVTPIRELDRVEIGA
                     GSRGPITEKIQSAFFDIVNGRNPKYAEWLTPV"
     gene            complement(2101..2520)
                     /locus_tag="VFV25_02170"
     CDS             complement(2101..2520)
                     /locus_tag="VFV25_02170"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY1392149.1"
                     /translation="MITAEEFNARGVGRLPGHLGLVVTESSEGRLRAQLEVQPHLLAP
                     NGYLHAGSVITLADTCAGYGCIAHLPEGASGFTTVELKSNHLGTALSGVIECVATPVH
                     LGRTTQVWDSVVTHRDTGKTIALFRCTQMVLYPKPDA"
     gene            complement(2517..3362)
                     /locus_tag="VFV25_02175"
     CDS             complement(2517..3362)
                     /locus_tag="VFV25_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEY1392150.1"
                     /translation="MKRHAKSITRLAAVSDIHGNLPALEAVLAEISRENVDLIVNLGD
                     IVSGPLWPVETAEHLMAMQLPTLRGNHERQLLHQPRERMSLSDAFAAERISTAQRQWL
                     DALPASLQLADDLLCCHGTPDSDLQYWLETVTDDFRSHGSRGVRMATTDEARARLGSF
                     AGHGASLVLCGHTHMPRTLQIPGGPLVVNPGSVGLQAYDDGNPYTHVMEVGSPHARWA
                     LIERSNGGWSVQLRATAYDWDAAAERAEANGRGDWADALRSGFVGRFEDEMLANIKSS
                     GKDHA"
     gene            complement(3393..3737)
                     /locus_tag="VFV25_02180"
     CDS             complement(3393..3737)
                     /locus_tag="VFV25_02180"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003068222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY1392151.1"
                     /translation="MSAIPGFAATPEPPYYAVIFSSRRTEGEQGYGAMADRMVELAAD
                     QPGFLGVESSRDAAGFGITVSYWRSEADIAAWKAQAEHREAQRLGHQKWYAHFELRIA
                     KVERAYGMGRPR"
BASE COUNT          616 a         1362 c         1365 g          696 t
ORIGIN      
        1 ccggcgatca gcgccatcgc ctcgtccagc gtcgtgcggc cggccatcac atggcaggcg
       61 ccaggcgcca gcagcgagat ctcgtcgcag atctcgcctt ccttggccga gcccagcagc
      121 agcaccggct ggcggctctg cgcgaaaagc tggcgcgcca aggccgcgta gttctcggaa
      181 ggccagcgct tggccgggcc gtattcagcg ccgggcgcga agacccagta ggcgcgccgg
      241 ctcacaccga cgcgctgcag caccacctcg acctgggccg gcggcaggct tagctgaggc
      301 ttgagcttgg tcgccgtgcg gcccgccagc gcgccatagt aggtcgccat tggcgggcgc
      361 tcctggccgc gcggcgggtt ggacaggcgc tcgttgagca gcagccagcg gccctcgcct
      421 tgatagccca gccgccgcgg aatgcgcgcc agccaaggca atagtgctga cttgatcgaa
      481 ttgggcagta cataggcgcg ctcgaagcgc ccgcgcagcg tggccgccat cgccttgcgc
      541 gcggcccagt ccagccggcc atgggcaaag ggcagctcga tcacctcgct cacctgcggc
      601 atcgcgcgca gcaccggcgc gacccagggc agcgccgcca ccgtcagcac ctcgccgcgc
      661 accgccaggg ccgcgagcaa gggctcggcc atcacggcgt cgccgatcca ttgcggagcg
      721 atgacgaggg agcgggtcat ctgcgctccg tcatatgcct agcgcacaaa gcgccaagca
      781 gacgccgcgg aaccggcttt gccgggccgc tggcggcgcc cccttgaggg ggagcgccgc
      841 aggcgctccg ggggtgggcc attagtgcgt atggagcttc tcgccggcct taagcttgta
      901 gacggtgccg caatacgggc acttgccctc gccttcggcg gccacgtcga tgtagacgcg
      961 cgggtgattg ctccacagcg gcatcttggg attggggcag aacaccacgc cagggccttg
     1021 caggtccttg gccgtcactt ccaccgtggc caccgcggtg gcagggctct tgttttcggt
     1081 actcatcgcg tgtttgtttc tcagaccggg gtcagccact cggcgtattt cgggttgcgt
     1141 ccgttgacga tgtcgaagaa ggcggactgg atcttctcgg tgatggggcc gcgcgagccg
     1201 gcgccgatct cgacgcggtc gagctcgcgt atcggcgtga cctcggctgc cgtgccggtg
     1261 aagaaggcct cgtcggcgat gtagacctcg tcgcgcgtga tgcgcttctc gaccagcttc
     1321 aggcccaggt cggcgcagat cgcaaacacg gtgttgcgcg tgatgccgtt gagcgcgccc
     1381 gcgctcaggt ccggcgtata gagcacgccg ttcttgacga tgaagaggtt ctcgccggcg
     1441 ccctcgctga cgaagcccga ggcgtcgagc agcatggcct cgtcataacc gtcctcggtc
     1501 gcctccatgt tggcgaggat ggaattggtg tagttcgaca ccgtcttggc ctgcgtcatc
     1561 gtgatgttga cgtgatggcg cgtgtagctg ctggtcttga cgcgaatgcc gcgcctcata
     1621 ccctcttcgc ccaggtaggc gccccaggcc caggcggcga ccatcaggtg gatggtgttg
     1681 cccttgggcg agacgcccag cttctcggag ccgatccagg tgagcgggcg caggtagcag
     1741 ctgtcgagct tgttggcgcg cacgacctgg cgctgcgcct ccatcacctg ctcgaagctg
     1801 taggggatct tcatgcgcag gatcttggcg ctgttgaaga gccgctcggt gtgctcccgc
     1861 aggcggaaga tggccgtcgt gccggcagcg gtcttgtagg cgcgcacgcc ctcgaaggcg
     1921 ccgcagccat agtgcagggt gtgggtcagc acgtggacct tggcgtcgcg ccagtcgacg
     1981 agttcgccgt ccatccagat cttgccgtcg cggtcgtcca ggcccatgaa tgcctccttg
     2041 cggtattgct gccagtggaa gcggcgattt tacgagccgc gcggcgatgc ccgccgtggc
     2101 tcaggcatca ggctttgggt aaagaaccat ctgcgtgcag cggaacagcg caatcgtctt
     2161 gcccgtatcc cgatgcgtga ccacgctgtc ccagacctgc gtggtcctgc ccagatggac
     2221 cggcgtcgca acgcattcga tgacgccgga cagcgccgtg cccaggtggt tggacttcag
     2281 ctcgacagtc gtgaagccgc tcgcaccctc gggcaggtgg gcgatgcagc catagccggc
     2341 gcaggtatcg gccagcgtga tcacgctgcc ggcgtgcaga tagccgttgg gcgccagcag
     2401 gtgcggctgg acttcgagct gcgcgcgcag cctgccctcg ctcgattcgg tgacgacgag
     2461 gcccagatgc cccggcaggc ggccgacgcc gcgggcgttg aattcttctg cggtgatcac
     2521 gcgtggtcct ttccgctgct cttgatgttg gccagcattt cgtcctcgaa gcgcccgacg
     2581 aagccgctgc gcagtgcatc ggcccaatcg ccgcggccgt tggcctcggc gcgctcggct
     2641 gcagcgtccc agtcgtaggc cgtggcgcgt agctgcacac tccagccgcc gttgctgcgt
     2701 tcgatcagcg cccagcgcgc atgcggactg ccgacctcca tcacatgggt gtagggattg
     2761 ccgtcgtcat aggcctgcag gccgacgctg ccggggttga ccaccagcgg gccgccgggg
     2821 atctgcagcg tgcgcggcat atgggtgtgg ccgcacagca ccaggcttgc gccatgcccc
     2881 gcaaaactgc ccagccgcgc gcgcgcctca tcggtcgtcg ccatgcgcac gccgcgcgag
     2941 ccgtggctgc gaaagtcatc cgtcaccgtc tccagccagt actgcaggtc gctgtcgggc
     3001 gtgccgtggc agcacagcag gtcgtcggcg agctgcaacg aggcgggcaa ggcgtccaac
     3061 cactggcgct gtgcagtgct gatgcgctcg gccgcaaagg cgtcgctcaa gctcatgcgc
     3121 tcgcgcggct ggtgcagcag ctgacgctcg tggttgcctc gcagcgtcgg cagctgcatg
     3181 gccatcaggt gctcggccgt ttccaccggc cacagcgggc cgctgacgat gtcgccgagg
     3241 ttgacgatca ggtcgacgtt ctcgcgcgag atctcggcga gcacggcttc cagcgccggc
     3301 agattgccgt ggatgtcgga cacggcggcc agccgcgtga ttgactttgc gtgccgcttc
     3361 atgatgatgc cccagcctca gctactttag gctcagcgcg gccgccccat gccataggca
     3421 cgctcgacct tggcgatgcg cagctcgaaa tgcgcgtacc acttttggtg ccccaggcgc
     3481 tgcgcctcgc gatgctcggc ctgggccttc caggcggcga tgtcggcctc ggagcgccaa
     3541 tagctcaccg tgatgccgaa acccgcggca tcgcgcgagc tctcgacgcc cagaaagccg
     3601 ggctgatccg ccgcgagctc gaccatgcga tcagccatcg cgccatagcc ctgctcaccc
     3661 tcagtgcgcc ggctgctgaa gatgaccgcg taatacggcg gctcgggggt agcggcgaag
     3721 ccggggatag cactcatggt cgctggcaga tgaagaaggg gcctccatcc tagccggcgc
     3781 tagcccgttc tgtttgctac cgaaattgtt ccatctgttg gcaaatattt tccagtaaca
     3841 ggcagaaaac tagccgaact gcaccgctct caggcaccag gcagctgcag caaggcacgc
     3901 aagcgacgca gctcgctggc atcgaagcgc ggctgcatcc attgctccat cgccgcctgg
     3961 cgctgcggcg gcgacgcgcc ggcgtcggcc tgcagctcgg cccaacgctg gcgcgcctcg
     4021 gccaggcggg cctgccagc
//
LOCUS       DASUCY010000110         4519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_34400, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000110 DASUCY010000000
VERSION     DASUCY010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34400"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="VFV25_02185"
     CDS             complement(<1..964)
                     /locus_tag="VFV25_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY1392152.1"
                     /translation="MQAPPSPPSMAQQLRLNLFGDRASALVSGAFLLGIAWALWELLR
                     WGVLGAEVQPEVAACRAAAGACWGVVAEKGRLVLLGRFPPGEQWRPIVGSLALLGCLG
                     FAAHPRGFGRRGLLALAAALVTFGVLMAGGLFGLTPVGTDLWGGLPLTLFLAVVACLL
                     GMPLGIALALARRSKLPALSWLATGFIEGVRGLPLITLLFFGAFVLPLVLPERWRVDP
                     MLRVGICLTMFCSAYLAEVVRGGLQAIGTGQYEAAHALGFTKWQMLTRIVLPQAMRIT
                     IAPTASLFIGAVKDTSLVAIVNLYDLTGTLRLAMGDSSWRPFFVE"
     gene            complement(968..2104)
                     /locus_tag="VFV25_02190"
     CDS             complement(968..2104)
                     /locus_tag="VFV25_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY1392153.1"
                     /translation="MSTAVAPPEQAAPPAAAINYRSLGLQALALAAVVLLGAWMVHNA
                     AVNLGARNIAAGFGFLKDSAGFAIAEGPVHYEPSDSYGRAFLAGFANTLRAAFPAVAL
                     ATVLGFALGIAQLSRHALVRGLSRTYVDIVRNVPLLVQLLLWYFALTELLPGSDAPVH
                     LGQAVFLSKGGAAFPAPEMSTGQTLVVLALTVALPVLAWRVKRWRAAAQAAAWLAVAA
                     MWVLVPEAWERPEQGVFGVSGGLALSPEWLALVMALSQYSGAYCAEIVRAGLLAVPRG
                     QWEAAHALGLTRWQTLRRLIVPQGLRVIVPPYTSLVMNTIKNSSLAIAVGYPDIVYIA
                     TTSLNQNGQAIECIAVIAAVYLALNLLTAVVMGFVNVRVQIKER"
     gene            complement(2127..3161)
                     /locus_tag="VFV25_02195"
     CDS             complement(2127..3161)
                     /locus_tag="VFV25_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY1392154.1"
                     /translation="MRTLVRSLALSAGLAAVFGLPTAAHAGKVLDGIKQKGVVTCGVH
                     TGRDGFALADSSGKWSGIDVDYCRALAAGVLGDANKVKYVPTSGQTRITALQSGEIDV
                     LARNTTWTYTRDTSLGLLWVGINFYDGQAFIAKKNPSLKSVKQLSGATICVDSGSTTE
                     KNLADYFQANKLKYKAVVYESSEASQQAFVSGRCQAYTGDSGNLAVFKATQVKNPDDY
                     VILPELISKEPVGPAVRRGDEDWFAIARWTLIAMIEAEELGISSANIDKLKAGSADPT
                     IKRFVGTGDDMGKFLGLDKDWSYRIVKQVGNYGESFDRNLGAGSKIKLPRGYMNLWTK
                     GGLMFAPPLR"
     gene            complement(3158..4015)
                     /locus_tag="VFV25_02200"
     CDS             complement(3158..4015)
                     /locus_tag="VFV25_02200"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEY1392155.1"
                     /translation="MSSAQSRVEAAAADASPSLSRVGQWIAAHPIQTLSHSAEEIAEL
                     TGTSVAAVNRFSRAAGFDGFGHLKSVLGEELQSAVEPLRKLDSRGRKEGKSAIDGHAL
                     DAAGRVPEVQRAAGRLLKAQQVWVLGMGASSHLAGYAAHVLMPYLPRVWAVAGEGGTE
                     EAARRLSRCGRADVLVAISLPRYSRDTVRLAAFARERGARVIAITDAPTAPLAAMADA
                     LLLAPSEHAVMPSSALGALAVIEALAAAVMRLNPDAARIARELSDAVVSHLAAPATSP
                     SASRKKESP"
     gene            4240..>4519
                     /locus_tag="VFV25_02205"
     CDS             4240..>4519
                     /locus_tag="VFV25_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392156.1"
                     /translation="MSHRTALAAGLAALSFTAAAIAADQTAPGAGNPYADRIAAASPR
                     VREAHVFLVEQAMKIRNAALRAATLDLLRNRGFCITSRAGVNDAVKSRM"
BASE COUNT          755 a         1578 c         1532 g          654 t
ORIGIN      
        1 tctcgacgaa gaaggggcgc cagcttgagt cgcccatcgc caggcgcagg gtgccggtga
       61 ggtcgtagag gttcacgatc gccaccagcg aggtgtcctt gacagcgccg atgaagaggc
      121 ttgctgtggg cgcgatggtg atgcgcatcg cctgcggcag cacgatgcgc gtgagcatct
      181 gccatttggt gaagcccaga gcgtgcgccg cctcgtattg cccggtgccg atcgcctgca
      241 ggccgccgcg aaccacctcg gccaggtagg cggagcagaa catggtcagg cagatgccta
      301 cgcgcagcat cggatccacc cgccagcgct caggcagcac cagcggcagc acgaaggcgc
      361 cgaagaacag cagtgtgatc agtggcaggc cgcgcacgcc ttcgatgaag ccagtggcca
      421 gccagctcag cgccggcagc ttggagcggc gcgccagcgc caaggcgatg cccagcggca
      481 tgcccagcag gcaggcgacc acggcaagga acagggtcag cggcaggcca ccccaaagat
      541 cggtacccac cggcgtcagg ccgaacaacc cgccggccat cagcacgccg aacgtcacca
      601 atgccgccgc cagcgccaac aggccgcggc gcccgaagcc gcgcgggtgg gcggcgaacc
      661 ccaggcagcc gagcagcgcc aggctgccga cgatggggcg ccactgctcg ccaggcggga
      721 aacgcccaag cagcaccagc cggcccttct cggcgacgac accccagcag gcgcccgctg
      781 cggcgcggca ggccgccacc tccggctgta cctcggcgcc gagtacgccc cagcgcagca
      841 gctcccacag cgcccaggcg atgcccagca aaaaagcgcc cgaaaccagc gcggaagccc
      901 ggtccccgaa caggttcagg cgcagctgct gcgccatcga tggaggtgaa ggcggcgcct
      961 gcatggctca acgctccttg atctggaccc gcacgttgac gaagcccatc acgacggccg
     1021 tcagcaggtt cagcgccagg tacaccgccg cgatcacggc gatgcattcg atggcctggc
     1081 cgttctggtt cagcgaggtc gtggcgatgt acacgatgtc cgggtagccc accgcgatgg
     1141 cgagcgagga gttcttgatc gtgttcatca cgaggctggt gtagggcggc acgatcacac
     1201 gcagcccctg cggcacgatc aggcggcgca gcgtctgcca gcgcgtgagc ccgagcgcat
     1261 gggcggcctc ccattggccc ctcggcaccg ccagcaggcc ggcgcgcacg atctcggcac
     1321 agtaggcacc ggaatactgg ctcagcgcca tcaccagcgc cagccactca gggctgagcg
     1381 ccagcccgcc gctgacgcca aagacaccct gctccgggcg ctcccaggct tcaggcacca
     1441 gcacccacat ggcagccacg gccagccacg ccgctgcctg cgccgccgca cgccagcgct
     1501 tgacacgcca cgccagcacc ggcagcgcga ccgtcagcgc gagcaccacg agcgtctgcc
     1561 ctgtcgacat ctccggtgcc gggaaggcgg cgcccccctt gctgaggaag accgcctgcc
     1621 cgagatgcac aggcgcgtcg ctgccgggca gcagctccgt caacgcgaag taccacagca
     1681 ggagctgcac cagcagcggc acgttgcgca cgatgtcgac ataggtgcgc gacagcccgc
     1741 gcaccagcgc atggcggctc agctgcgcga tgcccagcgc gaaacccagc acggtggcca
     1801 gcgccactgc ggggaaggcg gcccgcagcg tgttggcaaa gcctgcgagg aaggcgcgcc
     1861 cgtagctgtc gctcggctcg tagtgcaccg ggccttcggc gatcgcgaag ccggcactgt
     1921 ccttcaggaa gccgaagccg gcagcgatgt tgcgcgcgcc gaggttgacg gctgcgttgt
     1981 ggaccatcca cgcgcccagc aacaccaccg ccgccagcgc cagggcctgc agccccagcg
     2041 agcggtagtt aatggccgcg gccggcggcg ccgcctgctc ggggggcgca acggctgtag
     2101 acatgccagg ctgagtcatg gccggatcag cgcagcggcg gcgcgaacat caggccgccc
     2161 ttggtccaca ggttcatgta gccgcgcggc agcttgatct tggagcccgc gcccaggttg
     2221 cggtcgaagc tctcgccgta gttgcccact tgcttgacga tgcggtagga ccagtccttg
     2281 tccagtccga ggaacttgcc catatcgtcg ccggtgccga cgaagcgctt gatggtcggg
     2341 tcggcgctgc cggccttgag cttgtcgatg ttggccgagc tgatgcccag ctcctcggcc
     2401 tcgatcatcg cgatgagcgt ccagcgcgcg atcgcgaacc agtcctcatc gccgcgccgt
     2461 acggccgggc cgaccggttc cttggagatg agctcgggca ggatgacgta gtcgtcgggg
     2521 ttcttcacct gcgtcgcctt gaacacggcc aggttgccgc tgtcgccggt ataggcctgg
     2581 cagcgcccgc tgacgaaggc ctgctgcgag gcctcgctgc tttcgtacac cacagccttg
     2641 tacttgagct tgttggcctg gaagtagtcg gcgaggttct tctcggtggt cgagccggag
     2701 tcgacgcaga tggtcgcgcc gctgagctgc ttcacgctct tgaggctggg gttcttcttg
     2761 gcgatgaagg cctggccgtc gtagaagttg atgcccaccc acagcaggcc cagcgaggtg
     2821 tcgcgcgtat aggtccaggt cgtgttgcgc gccagcacgt cgatctctcc gctttgcagc
     2881 gcggtgatgc gcgtttggcc gctggtcggc acgtacttga ccttgttggc gtcgcccagc
     2941 acgccggcgg ccagcgcccg gcagtagtcg acatcgatgc cggaccactt gccggagctg
     3001 tcggcgagcg cgaagccgtc gcggccggtg tgcacgccgc aggtgacgac gcccttctgc
     3061 ttgatgccgt cgagcacctt gccggcatgg gcggcagtgg ggaggccgaa gaccgcggca
     3121 aggcctgcgg acagtgcgag ggagcggacg agggttctca tgggctttcc ttcttgcgag
     3181 aagctgaggg ggaggttgcg ggtgccgcga ggtgcgacac gacggcgtcg gacaactcgc
     3241 gcgcaatgcg cgctgcatcc gggttgaggc gcatgacggc ggccgcgagc gcctcgatca
     3301 cggccagcgc gcccagcgcg gagctgggca tcacggcgtg ttcgctgggc gcgagcagca
     3361 aggcatcggc catcgccgcc agcggggcgg tcggcgcatc ggtgatggcg atcacgcggg
     3421 cgccgcgttc gcgagcgaag gcggcgaggc gaacggtgtc gcgcgaataa cggggcaggg
     3481 aaatcgccac cagcacatcc gcgcggccgc agcgcgacag ccggcgcgcc gcctcctcgg
     3541 tgccgccctc cccggccacg gcccagaccc gcggcagata gggcatcagc acgtgtgcgg
     3601 cgtaaccggc gagatggctg ctggcgccca tgccgagcac ccagacctgc tgcgccttca
     3661 gtaggcgccc ggcggcgcgc tggacttccg gcacgcggcc ggccgcatcg agcgcatggc
     3721 cgtcgatggc ggacttgcct tccttgcggc cgcggctgtc gagcttgcgc agcggctcga
     3781 cagccgactg caactcctcg ccgagcaccg atttcaggtg cccgaagccg tcgaagcccg
     3841 ccgccctgga gaagcggttc accgcggcaa ccgaggtgcc ggtcaactcg gcgatctcct
     3901 ccgcgctatg agacagcgtc tggatgggat gcgcagcgat ccactgcccg acccgggaca
     3961 gggacggcga ggcatctgca gcagcggctt cgacacgact ttgggcggat gacatgaatg
     4021 aacaatatca ttcacttgat agaaagtaaa tcaattgatt cacttagtcc gctggtctgc
     4081 gcctgttcgg gcgcccggcc ttgccaccga gctgtcatct gtgcgactga cactgcggcc
     4141 gtctctcaag gaaacgatgg accgccccgt ttccttgacc ccctcggggg cgggctcgct
     4201 ttgccgagcc cttgggccat tcaacaagga gaacccaccg tgtctcaccg caccgccctg
     4261 gctgcaggtc tcgcagcgct ctcgttcacc gccgcggcga tcgccgccga ccagacggcg
     4321 cccggcgccg gcaaccccta tgccgaccgc atcgccgcgg cctcgccgcg cgtgcgcgag
     4381 gcccacgtct tcctggtcga gcaggccatg aagatccgca atgcggctct gcgtgccgcg
     4441 actctcgatc tgctgcgcaa ccgcggcttt tgcatcactt cccgcgccgg cgtgaacgac
     4501 gccgtcaaga gccgcatgc
//
LOCUS       DASUCY010000111         2046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1159104, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000111 DASUCY010000000
VERSION     DASUCY010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159104"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VFV25_02210"
     CDS             <1..255
                     /locus_tag="VFV25_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative dsRNA-binding protein"
                     /protein_id="HEY1392157.1"
                     /translation="LVAQTAAEAWTKDPKTELQEWLQARREPLPGYRVEATRGQQHDQ
                     VFVVLCSVPGPSSEARGEGRSRRAAEQEAARAALATLKSS"
     gene            252..1187
                     /gene="era"
                     /locus_tag="VFV25_02215"
     CDS             252..1187
                     /gene="era"
                     /locus_tag="VFV25_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEY1392158.1"
                     /translation="MSELSTHPNAEEGAAPQRCGLVAIVGRPNVGKSTLLNALVGQKV
                     SITSRKAQTTRHRITGIRTADEAQFVFVDTPGFQTRHGTALNRSLNRTVQGVLGDVDV
                     ILFVVEAGRYGADDAKVLALLQTDAMRGKPVILIANKLDQVRRRADLAPWLQGMQAHH
                     AFAEFVPLSAQREDDVERLLGIVKPYLPEQPWFYDEEALTDRSERFLASELIREKLFR
                     LTGDELPYTSTVVIEKFEEEGNLRRIAATIIVEREAHKGMVIGDGGERLKRIGSEARG
                     ELEKLLDAKVFLELWVKVRSGWADDEQRLRSYGYE"
     gene            1239..2006
                     /gene="recO"
                     /locus_tag="VFV25_02220"
     CDS             1239..2006
                     /gene="recO"
                     /locus_tag="VFV25_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEY1392159.1"
                     /translation="MATRTPVTAYVLHRYDWSETSLILDLFTREQGRVAVAAKGAKRP
                     YSQLRSVLLPFQRLQVSFGGRRSAPDGAQPEVQTLRAAEWAGGGAMLTGAALLTGFYL
                     NELLMRLLARHDPHAVLFDAYAQTLPLLAAQDDTVVESALRAFELLLLRETGVLPELS
                     QVTLTQQPVRAAATYMLRAEAGVAGAVAGEGGIPGQVLLALEAAMQSRELAALQHAAS
                     ASLPELKTQLRALLHYHLGSAALRTRQLMLDVQEQLG"
BASE COUNT          345 a          708 c          683 g          310 t
ORIGIN      
        1 ctggtcgcgc agaccgcggc cgaagcctgg accaaggacc ccaagaccga gctgcaggaa
       61 tggctgcagg cgcgccgcga gcccctgccc ggctaccgcg tcgaggccac gcgcgggcag
      121 cagcacgacc aggtcttcgt ggtgctgtgc agcgtccccg ggcctagcag cgaggcgcgc
      181 ggcgaagggc gctcgcgccg cgccgccgag caggaggcgg cacgcgccgc actcgctaca
      241 ctgaagtcct catgagtgag ctttcgacac acccgaatgc cgaagaaggc gctgcgccac
      301 agcgctgcgg cctcgtcgcc atcgtcggcc ggcccaatgt gggcaagtcg acgctgctga
      361 atgccctggt cggccagaag gtcagcatca cctcgcgcaa agcacagacc acgcggcacc
      421 gcatcacggg catacgcacc gcggacgagg cccagttcgt cttcgtcgac acgccgggct
      481 tccagacccg ccacggcacg gccctcaacc gcagcctcaa ccgtaccgtg cagggtgtgc
      541 tgggcgacgt ggatgtgatt ctcttcgtcg tcgaggccgg ccgctacggc gcggacgacg
      601 ccaaggtgct ggcgctgctg cagaccgatg ccatgcgcgg caagccggtc atcctgatcg
      661 ccaacaagct cgatcaggtg cgccgccgcg ccgatctggc gccctggctg caaggcatgc
      721 aggcgcatca tgccttcgcc gaattcgtgc cgctgtccgc tcagcgcgaa gacgacgtgg
      781 agcgcctgct cggcatcgtc aaaccttacc tgcccgagca gccctggttc tacgacgaag
      841 aggcactcac cgaccgtagc gagcgcttcc tcgcgagcga gctgatccgc gaaaagctgt
      901 tccgcctcac cggcgacgag ctgccctaca cctcgaccgt ggtgatcgaa aaatttgagg
      961 aagagggcaa tttgcgccgc atcgccgcca ccatcatcgt cgagcgcgaa gcgcacaagg
     1021 gcatggtgat cggcgacggc ggcgagcgcc taaagcgcat cggcagcgaa gcgcgcggcg
     1081 agctcgaaaa gctgctcgac gccaaggttt tcctggagct gtgggtcaag gtgcgctcgg
     1141 gctgggccga cgacgagcag cggctgcgca gctacggcta cgaatgaatc gccgcttcct
     1201 ttcgggccag cgaatcaatc tctgggtcta ggcggcacat ggcgacccgc accccggtga
     1261 cggcctatgt gctgcaccgc tatgactgga gcgaaaccag cctgatcctc gacctgttca
     1321 cgcgtgagca ggggcgggtg gcggtcgcgg ccaagggggc caagcgaccc tattcgcaac
     1381 tgcgctccgt gctgttgcct tttcaacgcc tgcaggtgag tttcggcggc cgccgcagcg
     1441 cgccggacgg ggcccagcca gaggtgcaga ccctgagagc cgccgaatgg gcgggcggcg
     1501 gcgccatgct gaccggcgcg gccctgctga ctggcttcta tctcaacgag ttgctgatgc
     1561 ggctgctcgc gcgccacgat ccccatgccg tgctgttcga cgcctatgcg cagacactgc
     1621 cgctgctggc tgcacaggac gacaccgtgg tggagtccgc tctgcgtgcc ttcgagctgc
     1681 tgctgctgcg cgaaaccggc gtcctgcccg agctgagcca ggtcacgctg acccagcagc
     1741 cggtgcgggc ggcggcgacc tatatgttgc gcgccgaggc cggcgttgcc ggcgccgtgg
     1801 cgggcgaggg cggcatccct gggcaggtgc tgctggcgct cgaggcggcc atgcaatccc
     1861 gcgagctggc agcgctgcaa cacgccgcca gcgcttcact gcccgagctc aagacccagc
     1921 tgcgtgcctt gcttcactat catctcggca gcgccgcgct gcgcacgcgc cagctgatgc
     1981 tggacgtgca ggagcagctg ggctgaatct gcctcgacga gatccaaatg aatcctctgg
     2041 tcgccc
//
LOCUS       DASUCY010000112         4165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_484350, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000112 DASUCY010000000
VERSION     DASUCY010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4165
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484350"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VFV25_02225"
     CDS             <1..529
                     /locus_tag="VFV25_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEY1392160.1"
                     /translation="EQLESPIYVVTGGGPGIMEAGNRGALDVGGKSIGLNIVLPHEQQ
                     PNPYITPELCFQFHYFGLRKMHFLMRSIALVCFPGGYGTLDEMFEVLTLVQTGKCRRR
                     PILLFGREFWSRLINFELLVETGMISPQDVGLFQYVETAEEAWAAIQRESSLDISQAG
                     SDTENNAASTTTTTP"
     gene            526..1443
                     /gene="rocF"
                     /locus_tag="VFV25_02230"
     CDS             526..1443
                     /gene="rocF"
                     /locus_tag="VFV25_02230"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HEY1392161.1"
                     /translation="MTVPKISLIGAPTDIGAGARGASMGPEALRVANIQPILESHGLE
                     VLDRGNLSGPTNPWLPPVDGYRHLSEVVAWNQAVHEAVHDELALGRLPILLGGDHCLG
                     IGSISAVARHCREKGRKLRVLWLDAHADFNTNILTPSGNIHGMPVACLCGRGPKELVE
                     IGGHVPALNPKWVRQIGIRSVDEGEKRLVHEVDLEVFDMRYIDEMGMRHTMELALATM
                     DSNTHLHVSFDVDFLDPDIAPGVGTTVKGGPTYREAQLCMEMIADTGRLASLDLMELN
                     PAFDIKNRTAELAVDLIESLFGKSTLMRK"
     gene            complement(1474..4026)
                     /locus_tag="VFV25_02235"
     CDS             complement(1474..4026)
                     /locus_tag="VFV25_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response DEXH box
                     helicase"
                     /protein_id="HEY1392162.1"
                     /translation="MKPGPAPAESWFAHRGWQPFAFQREVWAAMGEGRSGLLHATTGS
                     GKTYAVWFGALARTLDLAGARPRSEGAAQGGGAAPSTRGGPTAPALQVLWITPMRALA
                     ADTARALALPLPELAPSWSVGLRTGDTPASERARQDKRYPSVLVTTPESLSLMLTREQ
                     AREQLGGLQAIVVDEWHELIGNKRGVQLQLALARLKRWNPRLMIWGLSATLGNLQEAM
                     QVLLGRDDGLLVQGRIDKSLRIDTLIPQEPGRFSWAGHLGSQMQQPVIAEIERSATTL
                     VFTNVRSQAEIWYQLILRERPDWAGLVALHHGSLDKSVREWVEAGLKQGSLKAVVATS
                     SLDLGVDFLPVERVLQIGSAKGVARLLQRAGRSGHAPGRTSRVTLVPTHTMELVEAAA
                     ARRAAQAGRIEKRESPSKPLDVLVQHLVTVALGGGFEAEDLYAELRSAFAYRELTRSE
                     YQWALDFVEKGGASLGAYPEYHRVQQIDGIYRVPDRGIAKRHRLQVGTIVSEASMQVK
                     WMSGGTLGHIEEGFIARLKKGDCFVFAGRVLEYVMTRDMAAYVRRAAKPRGIVPAWSG
                     SRMPLSTEMADSVLELMQSAVQGDFFEPEMQAAAPMLRTQVRLSRLPTPHNLLVESHV
                     SREGHHLYLYPFAGRNVHLGLASLLAYRLARQQPNTFSIAINDYGFELLSALPVDIAA
                     LNGESAFSTERLLDDVLASLNSGELAQRRFREIARVAGLVFTGYPGAPKSTRQLQASS
                     SLFYEVFRKYDAGNLLLSQAENEVLSQELDIAKLRSTLRRMSSRRLEFVELSVPSPMS
                     LPLMVERFREQLSTEKLKDRLERILKDMSAAAEAVEAANTPPRARRRRAGQA"
BASE COUNT          680 a         1459 c         1384 g          642 t
ORIGIN      
        1 ggagcagctg gaatcgccga tctacgtagt caccggcggc gggccgggca tcatggaagc
       61 tggcaaccgc ggcgcgctcg acgtgggcgg caagagcatc ggcctcaaca tcgtgctgcc
      121 gcacgagcag cagcccaacc cttacatcac gcccgagctg tgcttccagt tccactactt
      181 cgggctgcgc aagatgcatt tcctcatgcg ctcgatcgcg ctggtgtgct tcccgggcgg
      241 ctacggcacg ctggacgaga tgttcgaggt gctgaccctg gtgcagaccg gcaagtgccg
      301 ccgccggccc atcctgctgt tcggccgcga gttctggtcc aggctgatca acttcgagct
      361 gctggtcgag accggcatga tcagcccgca ggacgtgggc ctcttccagt acgtggagac
      421 ggccgaggag gcctgggccg cgatccagcg cgaatcctcg ctcgacattt cgcaggccgg
      481 cagcgacacc gagaacaacg ccgcatcgac gacaacgaca acaccatgac cgtccccaag
      541 atcagcctca tcggcgctcc caccgacatc ggcgccggcg cgcgcggcgc ctccatgggc
      601 ccggaagccc tgcgcgtggc caacatccag cccatcctgg aaagccacgg cctcgaggtg
      661 ctggaccgcg gcaacctcag cggccccacc aacccctggc tgccgccggt ggacggctac
      721 cgccacttga gcgaggtcgt cgcctggaac caggcggtgc acgaggccgt gcatgacgag
      781 ctggcgctgg ggcgcctgcc catcctgctg ggcggcgacc attgcctggg gataggctcg
      841 atcagcgccg tggctcgcca ttgccgcgag aagggcagga agctgcgcgt gctctggctc
      901 gatgcgcatg ccgacttcaa caccaacatc ctcacgccca gcggcaacat ccatggcatg
      961 cccgtggcct gcttgtgcgg gcgcggaccc aaggagctcg tcgagatcgg cggccacgtg
     1021 cccgcgctca atcccaagtg ggtgcgccag atcggcatac gcagcgtcga cgaaggcgag
     1081 aagcgccttg tgcacgaggt cgacctcgaa gtcttcgaca tgcgctacat cgacgagatg
     1141 ggcatgcgcc acaccatgga gctggcgctc gcgacgatgg acagcaacac ccatctgcac
     1201 gtgagcttcg acgtcgactt cctcgacccc gacatcgcgc ccggcgtcgg caccaccgtg
     1261 aagggcggcc cgacctaccg cgaagcccag ctgtgcatgg agatgatcgc cgacaccggc
     1321 cgcctcgcct cgctggactt gatggagctc aaccccgcct tcgacatcaa gaaccgcacc
     1381 gccgagctgg cggtggacct gatcgagagc ctgttcggca agagcacctt gatgcgcaag
     1441 taaacagcag ccggcccacc ggtcgcggcc ggctcaggcc tgtccggccc gacggcgcct
     1501 ggcgcgcggc ggcgtattgg cagcctcgac cgcttccgcc gcagcgctca tgtccttcag
     1561 gatgcgctcg agacggtcct tcagcttctc agtgctcagc tgctcgcgga agcgctcgac
     1621 catcagcggc aggctcatgg ggctgggcac agacagctcg acgaattcca ggcgccgcga
     1681 gctcatgcgc cgcagcgtgc tgcgcagctt ggcgatgtcg agctcctggc tcaggacttc
     1741 gttttcggcc tggctcagca gcaggttgcc ggcgtcgtac ttgcggaaca cctcgtagaa
     1801 gagcgagctc gaggcctgca gctgcctcgt gctcttgggc gcgccggggt agccggtgaa
     1861 gacgaggccg gcgacgcgcg cgatctcgcg aaagcggcgc tgcgccaact cgccggagtt
     1921 gaggctggcg agcacatcgt ccagcagacg ctcggtgctg aaggcggact cgccgttcag
     1981 tgcggcgatg tcgaccggca atgcgctgag cagctcgaaa ccgtagtcgt tgatcgcgat
     2041 gctgaaggta ttgggctgct gcctggccaa gcgataagcc agcagagagg ccaaccccag
     2101 atgcacattg cgcccggcga aaggatagag atagaggtga tgcccttcgc gcgagacatg
     2161 gctctccacc agcaggttgt gcggcgtggg tagccgggac agcctgacct gggtccgcag
     2221 catgggcgca gccgcctgca tctcgggctc gaagaagtcg ccctggacag cgctttgcat
     2281 cagctccagc acggagtcgg ccatctcggt ggacagcggc atgcggctgc ccgaccaggc
     2341 gggcacgatg ccgcgcggct tcgcggcgcg gcgcacatag gcggccatgt cgcgcgtcat
     2401 cacgtattcg agcacgcggc cggcgaagac gaagcagtcg cctttcttga gtctcgcgat
     2461 gaagccttcc tcgatatggc ccagcgtgcc accgctcatc cacttcacct gcatcgaggc
     2521 ttcgctgacg atggtgccga cctgcaggcg gtggcgcttg gcgatgccgc ggtccggcac
     2581 gcggtagatg ccgtcgatct gctgcacgcg gtggtattcg ggataggcgc ccaggctggc
     2641 gccgcctttc tcgacgaagt ccagcgccca ttggtactcg gatcgcgtta gctcgcgata
     2701 ggcaaaggcg ctgcgcagct cagcgtagag atcttcggcc tcgaagccgc cgcccagggc
     2761 caccgtcacc agatgctgta ccagcacgtc cagcggcttg cttggcgact cgcgcttctc
     2821 gatgcgtccg gcctgggcgg cgcgccgcgc cgcggcggcc tcgaccaact ccatcgtgtg
     2881 cgtaggcacc agcgtgacgc gcgaggtccg gcccggcgca tggccgctgc gaccggcgcg
     2941 ctgcagcagc cgcgccaccc ccttggcgct gccgatctgc agcacgcgct ccacgggcag
     3001 gaagtccacc cccaggtcca gcgacgaggt cgcgaccacc gccttcaggc tgccctgctt
     3061 gagcccggct tccacccatt cgcgcacgct cttgtccagc gagccgtgat gcagggccac
     3121 caggccggcc cagtcgggcc gctcccgcag gatgagctgg taccagatct cggcctggga
     3181 gcgcacattg gtgaacacca gcgtcgttgc gctgcgctcg atctcggcga tgacgggctg
     3241 ctgcatctgc gagcccaggt ggccggccca ggagaagcgg cccggctcct gcgggatcag
     3301 cgtgtcgatg cgcagcgact tgtcgatgcg gccctgcacc agcaggccat cgtcgcgccc
     3361 cagcagcacc tgcatggcct cctgcagatt gcccagcgtg gcgctcaggc cccagatcat
     3421 cagccgcggg ttccagcgct tgagccgcgc cagcgccagc tgcagctgca cgccgcgctt
     3481 gttgccgatc agctcatgcc attcgtcgac cacgatggcc tgcaggccgc ccagctgctc
     3541 gcgcgcctgc tcgcgggtca gcatcaggct cagcgattcg ggcgtggtga ccagcacgct
     3601 gggatagcgc ttgtcctgcc gcgcgcgctc gctggccggc gtgtcgcccg tgcgcagccc
     3661 cacgctccag ctgggagcga gctcgggcaa cggcagcgcc agagcgcgcg ccgtgtcggc
     3721 cgccaaggct cgcatggggg ttatccacaa cacctgcaaa gcaggtgctg tggggccacc
     3781 cctggtggac ggggctgctc ccccaccctg cgccgccccc tccgagcggg ggcgggctcc
     3841 agccagatcg agggtacgcg ccaacgcgcc gaaccaaacc gcataggtct tgcccgagcc
     3901 ggtggtggca tgtagcaggc ccgagcgccc ctcgcccatc gcggcccaga cttcgcgctg
     3961 gaaggcgaag ggctgccagc cgcgatgcgc gaaccagctt tcggccggcg cgggcccagg
     4021 cttcattcgc ccagcattgc ggccggcagc ggcctcgtca cgcgcagcag gcgcatgccg
     4081 tcgaattcga gcagacggtt ggcggcaaag ctattctggg agtccggctc cagggcggcg
     4141 ctgatcagcg cggacagcgg ctggg
//
LOCUS       DASUCY010000113         2587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1384048, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000113 DASUCY010000000
VERSION     DASUCY010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384048"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VFV25_02240"
     CDS             <1..380
                     /locus_tag="VFV25_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY1392163.1"
                     /translation="RLQSLLRRSGMAARAAAAPAGGTGRVLHFEGLSLDTSRRVALDA
                     GGQELELTSTEYELLLMLAAEPGRVFSRDEILNQLRGHEADLYTRAVDILVSRLRKKL
                     SPLEAIKTLRNAGYCLALAPGVS"
     gene            503..1594
                     /locus_tag="VFV25_02245"
     CDS             503..1594
                     /locus_tag="VFV25_02245"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY1392164.1"
                     /translation="MAAHYGRRADGDAHAALHQWLREFEARAVYGLDERWAWRRRGRL
                     SLGGRLTLVFLLLAGAGSMLVHYTLQRSAAWPWLALGGLLLAVLAYASVRHMLRPMRA
                     LGWGVAAFGRGDLKHRIRVRHRDEIGALAARFNRMADDIQAMLDGKRALLLAISHELR
                     SPITRARLNAELLEEGTTREALVRDLALMRDLVEGLLERERLDSGHSALLLQDTDWTE
                     LVQDLIDRRYGAPLAEGALLLELEPALPTARLDRMRIQLLIGNLLDNALRYNERERGA
                     VRLSLKRDGKSLLLAVRDYGPGVAEDALAQLGLPFFRPDESRTRASGGVGLGLNLCRL
                     IAAAHGAQLEIRNADPGLEVAVRFGGAPT"
     gene            complement(1587..2564)
                     /locus_tag="VFV25_02250"
     CDS             complement(1587..2564)
                     /locus_tag="VFV25_02250"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEY1392165.1"
                     /translation="MEIFDYDNILLLPRKCRVVSRSECDASVEFGGRRFRLPVVPANM
                     KTVLDEPISQWLAANGYFYVMHRFDLDNVVYAKRMRDLGLFVSISSGVKAADYEAIDR
                     LAAEGVGADYITIDIAHGHAESVRKMIEHIKKKLPQAFVIAGNVGTPEAVIDLENWGA
                     DATKVGIGPGKVCITRLKTGFGTGGWQLSALKWCARVATKPIIADGGIRHHGDIAKSV
                     RFGAAMVMVGSLFAGHEESPGQTVEVDGRLYKEYYGSASDFNKGEYKHVEGKRILEPI
                     KGKLADTLREMQEDLQSSISYAGGRSLADLRKVNYVVLGGENAGEHLFM"
BASE COUNT          367 a          868 c          903 g          449 t
ORIGIN      
        1 cccggctgca aagcctgttg cgccgctccg gcatggccgc gcgtgcagcg gcggcgccgg
       61 ccggcgggac cggccgggta ctgcatttcg aaggcctgag cctggacacg agccggcgcg
      121 ttgcgctcga cgccggtggg caggaactgg agctcaccag caccgaatac gagctgctgc
      181 tgatgctggc tgccgaaccc ggccgcgtgt tcagtcgcga cgaaatcctg aaccagctgc
      241 gcggccacga ggccgatctc tacacgcggg cggtcgacat cctggtgagc cgcctgcgca
      301 agaagctctc gccgctggaa gcgatcaaga cgctgcgcaa tgcgggctat tgcctggctc
      361 tggcccccgg ggtgtcatga aagatcgttc ttcccaacat gattggcctc actggggcaa
      421 ggggccgtcg aacgagccgg actggcgcga gcgccgtcgc gagtttcgcc aggcgatgcg
      481 gcatggcggt tggcgccatt tcgtggccgc gcattacggt cgccgcgccg acggcgacgc
      541 gcacgccgcg ctgcaccaat ggttgcgcga gttcgaggcc cgggccgtct atggtttgga
      601 cgagcgctgg gcctggcggc gccgtggccg cttgtcgctg ggcgggcggc tgacgctggt
      661 cttcctgctg ctggccggag ccggcagcat gctggtgcac tacacgctgc agcggtccgc
      721 cgcttggccg tggctggcgc tgggcgggct gctcctcgcg gtgctggcct atgcgagcgt
      781 gcggcacatg ctgcggccca tgcgcgccct gggctggggc gtcgcggctt tcggccgggg
      841 cgacttgaag caccgcatcc gcgtgcgcca tcgcgacgag atcggcgcgc tcgctgcgcg
      901 cttcaaccgc atggccgacg acatccaggc gatgctcgac ggcaagcgcg cgctgctgct
      961 ggccatcagc cacgagctgc gcagccctat cacgcgggcg cggctcaacg ccgagttgct
     1021 ggaggaaggc acgacgcgcg aggctcttgt gcgggatctg gcgctgatgc gcgatctggt
     1081 cgaagggctg ctggagcgcg aacggctcga ctccgggcac tcggcgctgc tgctgcagga
     1141 caccgactgg accgagctgg tgcaggatct gatcgaccgg cgctacggtg cgccgctggc
     1201 cgagggcgcc ttgctgctcg aactggagcc ggcgctgccg acggcgcggc tcgaccgcat
     1261 gaggatccag ctcctgatcg gcaacctgct cgacaacgcc ttgcgctaca acgagcgcga
     1321 acgtggcgca gtgcggcttt cactgaagcg cgacgggaaa tcgctgctgc tggcggtgcg
     1381 cgactacggc cccggcgtgg ccgaggatgc gctggcgcaa ctgggcctgc ccttctttcg
     1441 gcccgacgag tcgcgcaccc gcgccagcgg cggcgtgggg ctcgggctga acctgtgtcg
     1501 gttgatcgcc gccgcgcatg gcgcgcagct cgagatccgc aatgccgatc cagggctgga
     1561 ggttgcggtg cgctttggcg gggcgcctac atgaacaggt gctcgcccgc gttctccccg
     1621 cccagcacca cgtagttcac cttgcgcagg tcggccaggc tgcgcccgcc ggcgtaagag
     1681 atggagctct gcaggtcttc ctgcatctcg cgcagcgtgt cggccagctt gcccttgatc
     1741 ggctccagga tgcgcttgcc ttcgacgtgc ttgtattcgc ccttgttgaa gtcgctggcc
     1801 gagccgtagt actccttgta gagcctgccg tcgacctcca ccgtctggcc gggcgactcc
     1861 tcgtggccgg cgaacagcga gcccaccatc accatcgcgg caccgaagcg cacgctcttg
     1921 gcgatgtcgc cgtggtggcg gatgccgccg tcggcgatga tgggcttggt cgccacgcgg
     1981 gcgcaccact tcagcgccga gagctgccag ccgccggtgc cgaagccggt cttgagccgc
     2041 gtgatgcaga ccttgccggg gccgatgccg accttggtcg cgtccgcgcc ccagttctcc
     2101 aggtcgatca ccgcctcggg cgtgccgacg ttgccggcga tcacgaaggc ctgcggcagc
     2161 ttcttcttga tgtgctcgat catcttgcgc acgctctcgg catggccgtg tgcgatgtcg
     2221 atggtaatgt agtcggcgcc cacgccctcg gcggccaggc ggtcgatcgc ttcatagtcg
     2281 gccgccttga cgcccgagct gatcgacacg aacagcccca gatcgcgcat gcgcttcgca
     2341 taaacgacgt tgtcgaggtc gaagcggtgc atcacgtaga aatagccgtt ggctgccagc
     2401 cattgcgaga tcggctcgtc gagcaccgtc ttcatgttgg ccggcaccac cggcagcctg
     2461 aagcggcgcc cgccgaattc caccgaggcg tcgcattcgc tgcggcttac cacgcggcac
     2521 ttgcgcggca gcaagaggat gttgtcgtag tcgaagattt ccatgatgtt caagagctcc
     2581 tggtgcg
//
LOCUS       DASUCY010000114         3113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_500510, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000114 DASUCY010000000
VERSION     DASUCY010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500510"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            334..588
                     /locus_tag="VFV25_02255"
     CDS             334..588
                     /locus_tag="VFV25_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392166.1"
                     /translation="MSSANDYGEHYWCVIGDGGSVFVYADRMEIAACGALTAWGGYRK
                     EGSHLAREQQLWAAAPGQWKTFFAASLVDGRAVALEAAAA"
     gene            complement(776..925)
                     /locus_tag="VFV25_02260"
     CDS             complement(776..925)
                     /locus_tag="VFV25_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392167.1"
                     /translation="MALDHHSSSPAGRTPLRVWLTAGLLGMAAIGVAAWWLTASQSPW
                     TSPSE"
     gene            1083..2282
                     /locus_tag="VFV25_02265"
     CDS             1083..2282
                     /locus_tag="VFV25_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEY1392168.1"
                     /translation="MARTRKALPARVDVAHLISAGVLAAVCPRPLIEQVLAETGKASQ
                     RERLLPAPAVVYYVMALALWREAPLEEVLRVVCEGLQWLGGGRGQAVQASKSAISQAR
                     TRLGPQVMQRLAQAVLRPLAEPGAPGAWYRGLRVMALDGSCLDVADEQANAEFFGYPG
                     APRGQSAFPQARVLGLVECGTHAIVAADVAPYKHNSEKAMAAAVLPGWLRPDMLVLAD
                     RGFYSFKLWQRAAATGAMLLWRVSATIKLPAQQLLADGSYLSTIYDSADSARRHGVTV
                     RVIEYALQDSATPTEDSYRLITTFDADQAPALELAALYHERWEIEGVFDEFKTHLRGS
                     STVLRSKTPDLVQQELWGLLLAHFAVRQLMAQAAWRQELDPDRLSFMHAVRVIKRKLP
                     QAAAVPP"
     gene            complement(2473..>3113)
                     /locus_tag="VFV25_02270"
     CDS             complement(2473..>3113)
                     /locus_tag="VFV25_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392169.1"
                     /translation="TRPADAWAFIQGTWARLREQLGGMGLNLPEIVSASAAAAASASP
                     STAAFAHSAAAPLAPAAPVLDMRPMPELPAQRTAVPAAEAMGSREALLLRYARRCAEI
                     KGMLSVCIFDAAAQRPLAHAGSRPGPASLATQGAALQAAIAEAGRVLGLSTGAVDAAV
                     TLDQHHLLLRSVPQHDGLLLHAVLDKGVANLTLARLQLGRLDAVLEDPSGAV"
BASE COUNT          498 a         1004 c         1150 g          461 t
ORIGIN      
        1 ggtggccatg gacgacaggt cagtgagggt cagtgagcca aagctgtctc tcgagccgga
       61 aggtcagccg tccgttcttg agcggccaga cggcgacgaa tcctgtgcag ccgttgccga
      121 cccttagccg gcgtacagac cgtcgagcca cctgccggta agctgacctt ccgcctcccg
      181 cacatacaag gggttaggcc gaggttaggc cgcagtatgc gcgcgcgccc agcgtgaaca
      241 gcctgccatt cagagccaga agcgcagaat cgttctggcg gcgggttcgt tcgggactgg
      301 cttgggcggg tcctggcgcc gatggagttg aacatgagct ctgcaaacga ctacggtgag
      361 cactactggt gtgttatcgg cgatggcggt tctgtcttcg tctatgccga taggatggag
      421 attgcagcat gtggcgcgct gaccgcgtgg ggcggctatc gaaaggaggg cagccacctt
      481 gcgcgtgagc agcagttgtg ggcggccgcg cccggccagt ggaagacgtt ctttgctgcc
      541 agtctcgtcg acggaagagc agtagcactc gaagccgccg cggcgtgacc cgaggtggca
      601 tcgggttgtg tggcggcgga cgagggtgaa gcagcatgca ttctttcagc cggggtggta
      661 ccgctcacgg ccctctgcgg cctaacgaat gaaagggact tccccgtccc gaactagccg
      721 cgtagcggca accggcatcg aagtgccacg atgggaagtg cgaactctca tcaactcact
      781 cggaagggga agtccatggc gattgcgacg cggtcagcca ccaggccgcg acgccgatcg
      841 cggccatgcc gagcaggcca gccgtcagcc acacgcgcag cggggtgcgc ccggcgggcg
      901 aggaggagtg atgatcgagt gccattggaa cttcctgggc atgtgcgagc ggtttgtggt
      961 tgtggttcaa cgcgccgcgg caggcgacgc ggcatcgatc gaaacatggt ctagttccaa
     1021 tactgttcag atagataata ttgtgcgata ctccggttcc gtctccactg cggacggaat
     1081 cgatggcacg aacacgcaag gcgctgccgg cgcgggttga tgtggcgcac ctgatcagcg
     1141 ccggggtgct ggcggcggtg tgcccgcgcc cgctgatcga gcaggtgctg gccgagacgg
     1201 gcaaggccag tcagcgcgag cgactgctgc cggcgccggc ggtggtgtac tacgtgatgg
     1261 cgctggcgct gtggcgcgag gcgccgctgg aggaagtgct gcgcgtggta tgcgagggac
     1321 tgcagtggct cggcggcggc cgcgggcagg cggtgcaggc cagcaagtcc gcgatctcgc
     1381 aggcgcgcac ccggttgggc ccgcaggtca tgcagcggct ggcgcaagcg gtgctgcgcc
     1441 cgctggctga gcccggggcg ccgggcgcgt ggtaccgggg gctgcgcgtg atggcactgg
     1501 acggcagctg cctggacgtg gccgacgagc aggccaacgc cgagttcttc ggctacccgg
     1561 gggccccgcg cggacaaagc gccttcccgc aggcgcgcgt gctcgggctg gtggaatgcg
     1621 gcacgcacgc catcgtcgcg gccgatgtcg ccccgtacaa gcacaacagc gagaaggcga
     1681 tggctgccgc ggtcctgccc ggctggctgc gcccggacat gctggtgctg gccgaccggg
     1741 gcttttacag cttcaagctg tggcagcgcg ctgccgccac cggcgcgatg ctgctgtggc
     1801 gagtgagcgc cacgatcaag ctgccggcgc agcagctcct ggccgacggc tcgtacctga
     1861 gcacgatcta cgacagcgcc gacagcgccc gccgccatgg cgtgaccgtg cgcgtcatcg
     1921 aatacgcgct gcaggactcg gccaccccca ccgaggacag ctatcgattg atcaccacct
     1981 tcgacgccga tcaggcgccg gccctggagc tggcggcgct gtaccacgag cgctgggaga
     2041 tcgagggcgt gttcgacgag ttcaagacgc acctgcgagg cagcagcacg gtgctgcgca
     2101 gcaagacgcc cgatctggtg cagcaggagc tatggggact gctgctggcg cacttcgcgg
     2161 tgcgccagct gatggcgcag gcggcctggc gccaggagct cgaccccgac cgattgagct
     2221 tcatgcacgc ggtgcgcgtg atcaagcgaa aactgccgca agccgcggcc gttcccccct
     2281 gagcagcttg aagactggcg cgacgcgctg ctggccgaga tcggtcgcgg gcgctgcgtc
     2341 agcagccgcg gacgcagcaa cccgcgcggc gtcaagcgca agatgagcaa cttcaaggtt
     2401 cgccgccgcg gccaggcgct gcatcggcgg catcagccga cgccggtgtt acgtatctga
     2461 acagtattgg cactagaccg cgcccgaagg gtcttcgagc accgcgtcca gccggccgag
     2521 ctgcaggcgc gccagcgtca ggttggcgac gcccttgtcg agcaccgcgt gcagcagcag
     2581 cccgtcgtgc tgcggcaccg agcgcagcag caggtggtgc tggtcgagcg tgacggcggc
     2641 gtcgaccgcg ccggtggaca ggccgagcac gcggcccgcc tcggcgatgg ccgcctgcag
     2701 cgcggccccc tgcgtggcca gcgatgccgg cccggggcgc gagcccgcat gcgcgagcgg
     2761 ccgctgagcg gcggcgtcga agatgcacac gctgagcatg cccttgatct cggcgcagcg
     2821 gcgcgcatag cgcagcagca gcgcctcgcg cgagcccatc gcctcggccg cgggcactgc
     2881 ggtgcgctgc gcgggcagct ccggcatcgg ccgcatgtcg agcacgggcg cggccggagc
     2941 cagcggtgcc gcggcgctgt gtgcgaaggc agcggtcgag ggcgatgcgc tggccgccgc
     3001 agcggcgctg gcgctgacga tctccggcag gttcaggccc atgcccccca gctgctcgcg
     3061 cagccgcgcc caggtgccct ggatgaaggc ccaggcgtcg gccgggcgcg tga
//
LOCUS       DASUCY010000115         2847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1319801, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000115 DASUCY010000000
VERSION     DASUCY010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1319801"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /locus_tag="VFV25_02275"
     CDS             <1..1066
                     /locus_tag="VFV25_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392170.1"
                     /translation="AEAGQVGNTQAGGQSTLDSRVAGGEDAPDHPWAESPAATPATPL
                     LQAAAMTLDVNSRPPDSQPDAERPAAAADAFTPLALRRHAADQDERDAIGRLNFLHGA
                     GERRAALLALILTPGSAREKQAWREETAEVASAGRVRADVARLSAASRLPVFELLLQR
                     SRNVPVAERQALVEAARCVMGADGQVRPIDRLHWLAMRHVLGEAGATPVAASGSDNDP
                     AALPLASVEQFASLTAWLARLVPAPVDLNAKGAVSKAGNAWYESAISQWVQRYRAQGR
                     RAPACHPPDADGMAHALWAVQALSWMLKPVLLRAWVDAAVSMSAKATLLPEAADALRL
                     LALLLDTPMPPALAEHFLEV"
     gene            complement(1142..1363)
                     /locus_tag="VFV25_02280"
     CDS             complement(1142..1363)
                     /locus_tag="VFV25_02280"
                     /inference="COORDINATES: protein motif:HMM:NF020265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HEY1392171.1"
                     /translation="MSLHATVEFYAPSDELDLIDLAARVVGISRGEFLLHSAAERAHE
                     VLLDGAELEVDEEASRHTIELAEAARMVC"
     gene            1513..>2847
                     /gene="rsmB"
                     /locus_tag="VFV25_02285"
     CDS             1513..>2847
                     /gene="rsmB"
                     /locus_tag="VFV25_02285"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEY1392172.1"
                     /translation="MSAKPPSVPLATLLAHTADAVAAVRAGRSLNDALAGCPGSARPG
                     TQALAFQTLRQLGRALALRAELAKKPPAAWTDALLLTALALVWDEAQAPYAPHTLVDQ
                     AVEAAKHRARQQSGFVNAVLRRFLRERAALVAATEADEEARWNHPRWWVEMLKRDWPA
                     QAEALLAANNARPPMTLRVNARRGDAAAYLQRLAEAGIPAQATGAHTVVLAQPQPVQV
                     LPGFADGAVSVQDAAAQLAAPLLIGSGASALAPGARVLDACAAPGGKTAQLLELADLD
                     VLALDVDEERLQRVQETLQRLALRAELKAADAGRPETWWDGRPFDAILLDAPCSASGI
                     VRRHPDVRWLRRAEDLVALTRVQAELLDALWPLLKPGGRLLYATCSIFKAEGLAQIDA
                     FLQRQPAAVLAADPPSPGHLLPLPDNGAHRMPAGPAASAALHDGFFYALLQKH"
BASE COUNT          409 a         1024 c          993 g          421 t
ORIGIN      
        1 ggcagaggcc gggcaggttg gcaacacgca agcaggtgga cagtccactc tggacagccg
       61 ggtcgcggga ggcgaggatg cgccggatca tccctgggcc gaatcgcctg cggccactcc
      121 cgctacacct ttgttgcaag ccgccgccat gactctggac gtgaattccc ggccgccgga
      181 cagccagccc gatgccgagc ggcctgccgc agctgccgac gctttcacgc ccctggcctt
      241 gcgccgacat gctgccgacc aggacgagcg ggacgcgatc gggcggctga acttccttca
      301 cggcgcgggc gagcgccgcg cagccctgct ggcgctcatc ctgacgcccg gcagcgcgcg
      361 cgagaaacag gcctggcgtg aggagacggc ggaagtcgcc tcggccggcc gggtgcgcgc
      421 cgacgtggcg cggctgagcg cggcgagccg gctgccggtc ttcgaactgc tgctgcagcg
      481 atcgcgcaat gtgccagtgg ccgagcgcca ggcgctggtc gaggcggccc ggtgcgtcat
      541 gggcgccgat ggccaggttc ggccgataga ccgcctccac tggctggcga tgcgccatgt
      601 cctgggtgag gcgggcgcga ctcccgttgc cgcctccggc agcgacaacg accctgccgc
      661 gctgccgctg gccagcgtcg agcaattcgc cagcctcacc gcctggttgg cgcggctggt
      721 gccggcgccc gtggatctga acgccaaggg cgccgtcagc aaggccggca acgcctggta
      781 tgaatccgcg atatcgcagt gggtacagcg ctatcgcgcg caagggcgca gggcgcctgc
      841 ctgccatccg cccgatgccg acggcatggc ccatgcgcta tgggccgtgc aggcgctgtc
      901 ctggatgctc aagccggtcc tgttgcgggc ctgggtcgac gcggccgtga gcatgagtgc
      961 caaggctacg ctgctgccgg aagcggccga tgcgctgagg ctgctggccc tgctgctgga
     1021 tacgccgatg ccgccggccc tggccgagca ttttctggag gtctagggcc tgaaacgacg
     1081 aggcccgcct accagcttac gggtagcggg ccccgagtcc tgcggggcga atcgccgctc
     1141 gtcagcacac catgcgcgca gcctcggcca gctcgatggt gtggcggctc gcctcctcgt
     1201 cgacttccag ctccgcgccg tccaacagca cttcatgcgc acgctcggcg gcggagtgca
     1261 gcaggaactc cccacggctg atgcccacca cgcgggcggc aagatcgatc agatccagct
     1321 cgtcgctggg tgcatagaac tcgaccgtag cgtgcaggct catggctttc cccttgtgtt
     1381 gcagtctaag gagatcgccc gcttcatgct tcccctgttc gagggaagca acaacagagt
     1441 gaggattttt gtcgtgcaga aaaccacagt tttcgggccg gtcccgcttg cacgcgcata
     1501 gactcccgcc ccgtgagtgc caagccaccc tctgttccgc tcgccacgct gctggcccac
     1561 acggccgatg cggtggcagc ggtgcgcgca ggccgttcgc tgaacgacgc cctggcgggc
     1621 tgcccaggca gcgcgcgccc cggcacccag gccttggcct tccagaccct gcgccagctg
     1681 ggtcgtgccc tggccttgcg cgccgagctc gccaagaagc cgcccgcagc ttggaccgac
     1741 gccttgctgc tgacggcgct ggctctggtc tgggacgagg cccaggcgcc ctatgcaccc
     1801 cacaccctgg tggatcaggc ggtggaggcc gccaagcacc gcgcccgcca acaatcaggt
     1861 ttcgtcaacg cggtgctgcg gcgcttcctg cgcgaacgcg cggcactggt ggcggcgacc
     1921 gaggccgacg aagaggcccg ctggaaccat ccgcgctggt gggtggagat gctgaagcgc
     1981 gactggccgg cccaggccga agccttgctc gccgccaaca acgccaggcc gccgatgacg
     2041 ctgcgagtca acgcccgccg gggcgacgct gccgcctatc tgcagcgcct cgccgaggcc
     2101 ggcatcccgg cccaagcgac gggagcgcac acggtggtgc tggcccagcc ccagccggtg
     2161 caggtcttgc cgggtttcgc cgacggtgcg gtgtcggtgc aggatgcggc ggcccagctg
     2221 gccgcacccc tgctgatcgg aagcggggcg agcgcgctcg cgcctggggc ccgggtgctc
     2281 gacgcctgtg ccgcgccggg cggcaagacg gcgcagctgc tggaattggc ggatctggac
     2341 gtgctggcgc tggacgtcga cgaagagcgc ctgcagcgcg tgcaggaaac gctgcaacgc
     2401 ctggccttgc gggccgagct caaggcggcc gacgccggcc gacccgagac ctggtgggac
     2461 ggccgcccct tcgacgccat tctgctggac gccccctgca gcgcctccgg catcgtgcgc
     2521 cgccacccgg acgtgcgctg gctgcgccgc gccgaggacc tggtcgcgct gacgcgcgtg
     2581 caggccgagc tgctcgatgc gctgtggccg ctgctgaagc ccggcgggcg actgctgtac
     2641 gcgacctgct cgatcttcaa ggccgaaggg ctggcgcaga tcgacgcttt tttgcaacgt
     2701 caacccgcag cagtgctcgc cgccgacccg ccgtcgcccg ggcacctgct gcccctgccc
     2761 gacaatgggg ctcatcgaat gccggccggc cccgccgcct ccgcggcgct gcacgacggc
     2821 ttcttctacg ccctattgca aaagcat
//
LOCUS       DASUCY010000116         4241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_452409, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000116 DASUCY010000000
VERSION     DASUCY010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4241
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452409"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            348..770
                     /locus_tag="VFV25_02290"
     CDS             348..770
                     /locus_tag="VFV25_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY1392173.1"
                     /translation="MKILLAVDGSSYTKQMLAYLAAHDEWLGTAHQYTVAYAQLELPN
                     GLLALLSEAAIRERHEADAEMVFKPIRSFLEMQSIHAQFVHQVGAPGKVIAGLAEAGK
                     FDLIMMGSHGHGALGGMVLGSTTTKVLALCKTPVLVVR"
     gene            complement(784..1524)
                     /locus_tag="VFV25_02295"
     CDS             complement(784..1524)
                     /locus_tag="VFV25_02295"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEY1392174.1"
                     /translation="MNVILLSIVTFFSTAAGGLCAFRFRDRLHLVLGFTAGVLLGVVS
                     FDILPEIFELAHAQGSNGLGAMVALVAGFLLFHSLEKFVLVHHAHENQYAHHHHPKVG
                     VLSALALIGHSFMDGVGIGLAFQVSNALGITVAIAVIAHDFCDGLNTVSLMLLHRNST
                     RSSMVMLILDALAPVLGAASTLAFQVPAPTLLLYLGFFAGFLLYIGASDILPEAHSRA
                     RPGTSLGLIALTTLGAVLMYFVLRVHPG"
     gene            complement(1561..2751)
                     /locus_tag="VFV25_02300"
     CDS             complement(1561..2751)
                     /locus_tag="VFV25_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017675698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035444 - nickel cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux transporter"
                     /protein_id="HEY1392175.1"
                     /translation="MPPIADLIQQGSTNLLLFIPTAILLGALHGLEPGHSKTMMASFI
                     IAVRGTMGQAALLGLCAALSHSLIVWALAALALKLGNQLIAEQAEPYFMVASAVIVIG
                     VAAWMFMRTRRDDLAAQAQRHAPHGGRIINTGHGVVELLVFEDGVPPRFRLHAYDHHM
                     QPQAFEPGMTITLATIRADGGRQEFSLEAKDGYLESRETIPEPHEFEVRLSLGHGDHA
                     HDFEVRWSENEEEGGHAGHGHSHGHDHDHGDDHGHSHGHSHSHSAAFASGGGYQDAHE
                     RAHAEDIKRRFAGRQVTTGQIAMFGLTGGLVPCPASVTILLICLHLKRFSLGAAMVAS
                     FSLGLAIALVGVGVIAAWGAQQVGKRFGNLGNLARKMPYLSSLLMMLVGAVMGWQGLS
                     SILA"
     gene            2855..3130
                     /locus_tag="VFV25_02305"
     CDS             2855..3130
                     /locus_tag="VFV25_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015856024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal/formaldehyde-sensitive transcriptional
                     repressor"
                     /protein_id="HEY1392176.1"
                     /translation="MPHTIKGKKQLLARVRRIAGQVGALERALEAESEGSDVLQQIAS
                     IRGAVNGLMAEVLEHHLRDHLAADNLDPQQRGQDLEQVVTVLRSYLK"
     gene            3278..3880
                     /locus_tag="VFV25_02310"
     CDS             3278..3880
                     /locus_tag="VFV25_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY1392177.1"
                     /translation="MLRRRDENGRHGDEASAAEQAEVRLIARVAARELPAFEELYRCY
                     HPRLTRFLDRVTRRPSLVEELLNDTMLVVWNRADSYNGRSKLSTWIFAIAYRKALKAL
                     QGLDEAVEDPQADQRAASGPGPEQQAGQGELRAVLARAMGELSAEHRAVVDLTYFHGI
                     GYREIAQIVECPVDTVKTRMFYARRRLRDLLDGQLQDWLP"
     gene            3890..>4241
                     /locus_tag="VFV25_02315"
     CDS             3890..>4241
                     /locus_tag="VFV25_02315"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEY1392178.1"
                     /translation="MSARVVKLDADTHRSVQALLPWYVTQRLGAEDLAKVETHLAACP
                     HCQAELAWERRLHAARLEHDDFRAHGDAEAGLALLRSRIEASRSEQPRARLRRLRDGV
                     RAALRDWRLAWQGSA"
BASE COUNT          797 a         1351 c         1442 g          651 t
ORIGIN      
        1 tcacgaatga agaaccgccg aagcctccgg aggggccgaa ttccgtcatc tctgcggcgc
       61 gagcggccgg cggcaagagc atcgccagca aggctgcaaa ggcgagcgcg gcagcttgcg
      121 caatgcggtg gcatgtgtcc atcgggccct cctcggcgtc acggaagctg aatatgcagc
      181 ggctgcggtt gtactgcagc ccggctcagc acgggatccc caaaaggggt gtgccgctct
      241 tgagctaggt caagctgccg atcacgctga caatgcgacg attctgcatc cgtcgcatgt
      301 cctggtcacg tcgctgcggg atgtgtgcgc accgaaggaa cagcaccatg aagatactct
      361 tggccgttga cggcagctcc tacaccaagc aaatgctggc ctatctggcc gcacacgacg
      421 aatggctggg caccgcgcac cagtacaccg tggcctatgc ccagctggag ctgccgaacg
      481 gcttgctcgc tttgctcagc gaggctgcca tccgggagcg tcacgaggcc gacgcggaga
      541 tggtcttcaa gcccatccgc agcttcctcg agatgcagtc catccatgcg cagttcgtcc
      601 atcaggtcgg tgcgccgggc aaggtcatcg cgggcctggc cgaggccggc aagttcgact
      661 tgatcatgat gggctcgcac ggacacggcg ccctgggagg catggtcctg ggctccacca
      721 ccaccaaggt gctggcgctg tgcaagacgc cggtgctggt ggtgcgctga cggggaaccc
      781 gcttcagccc gggtgcacgc gcaacacgaa atacatcagc acggcgccca gcgtggtcag
      841 cgcgatgagg cccagtgagg tgcccggccg ggcccttgag tgcgcctcgg gcaggatgtc
      901 cgaggccccg atatacaaca ggaagcccgc aaagaagccc aggtagagca gcagcgttgg
      961 cgcgggaacc tggaaagcca gcgtcgaggc ggcgcccagg accggcgcca gggcgtccag
     1021 tatcaacatc accatcgagc tgcgcgtcga gttccgatgc aacagcatca ggctgaccgt
     1081 gttgaggccg tcgcagaaat cgtgggcgat gaccgcaatg gcgacggtga tccccagcgc
     1141 attcgaaacc tggaaggcca ggccgatgcc tacgccatcc atgaagctgt ggccgatcaa
     1201 ggccagggcc gacagtacgc cgactttggg gtgatggtgg tgggcgtact ggttctcgtg
     1261 cgcgtggtgc accagcacga atttctcgag gctgtggaaa agcaggaagc ccgccaccag
     1321 tgcgaccatc gcgcccaggc cgttgctgcc ctgcgcgtgc gccagctcga agatctccgg
     1381 caggatgtcg aagctcacca cgcccagcag cacaccggcc gtgaagccca gcacgagatg
     1441 aagccggtcg cggaatcgga aggcgcacag gccgccggcg gcggtggaga agaaggtaac
     1501 gatcgacagc aggatgacgt tcaagaggcg gctttctgcg tgttcttttg cggcaaaggc
     1561 tcaagccagg atgctgctca agccctgcca gcccatgacg gcgccgacca gcatcatcaa
     1621 caagctggac aagtaaggca ttttgcgggc aagattgccc aggttgccga agcgcttgcc
     1681 cacctgctgc gcgccccagg cggcaatgac gcccacgccg accagcgcga tggcgagccc
     1741 gaggctgaac gaggccacca tcgccgcgcc caggctgaaa cgcttcaaat gcaggcagat
     1801 cagcaatatc gtgaccgagg ccgggcaagg aacgaggcca cccgtcaggc cgaacatcgc
     1861 gatctggccg gtcgtgactt gccgccccgc gaagcggcgc ttgatgtcct cggcgtgcgc
     1921 ccgttcatgg gcgtcctgat aaccgccgcc tgaagcgaag gccgccgagt gactgtggga
     1981 atggccgtga gagtggccgt ggtcatcgcc atgatcatgg tcgtggccat gactgtgacc
     2041 gtggcccgca tgcccgccct cctcctcgtt ctcgctccag cgcacttcga agtcatgggc
     2101 atggtcgcca tggcccaggc tcagccgcac ctcgaactcg tgcggctcgg gaatggtctc
     2161 gcgggattcg aggtagccat ccttggcctc cagcgagaac tcctggcgac cgccgtcggc
     2221 gcgtatggtc gccagcgtga tcgtcatacc cggctcgaag gcctgcggct gcatgtggtg
     2281 gtcataggca tggagccgaa agcgcggcgg tacgccgtct tcgaagacca gcagttccac
     2341 cacgccgtgc ccggtgttga tgatgcgccc gccatgcggc gcatggcgct gcgcctgggc
     2401 tgccagatcg tcccggcgcg tgcgcatgaa catccatgcc gcgacgccga tgacgatgac
     2461 cgccgaggcc accatgaaat agggctcggc ctgctcggcg atcagctgat tgccgagctt
     2521 gagcgccagc gccgcgagcg cccagacgat cagcgaatgc gacagcgccg cgcaaaggcc
     2581 cagcagggct gcttgcccca tggtgccgcg cacggcgatg atgaaggacg ccatcatggt
     2641 cttggaatga ccgggctcca gcccgtgcag agctcccagc aggatggcgg tggggatgaa
     2701 cagcagcagg ttggtcgatc cctgctggat cagatcggcg atgggcggca tgagggctcc
     2761 gggagatggg aaggcagaac caatggggtg acccatcgag atcgcagttt gcatatacta
     2821 taccccacta ccctaaatcg gagcgcaaag agccatgcct cacaccatca agggcaagaa
     2881 gcagctgctg gcccgcgtgc gccgcatcgc aggccaggtc ggtgccctgg agcgggcact
     2941 ggaggccgaa tccgagggct ccgacgtgct gcagcagatt gcgtcgatac gcggcgcggt
     3001 gaacggcctg atggccgagg tgctggagca tcatctgcgc gaccacctcg ctgcggacaa
     3061 cctggacccg cagcagcgcg gtcaggacct tgagcaggtc gtgaccgtat tgcgctccta
     3121 cttgaagtga ccatgccagc gttcgcgcgg cggggttagc gacaccgcca ggggtgcctt
     3181 tggcttgcgc cgagcggcgc cgctgcgcac actccaggca tcggattgaa cctttttcgc
     3241 gccggcggga atcgacggcc cggaggagca cgcaccgatg ctgcgcaggc gcgacgagaa
     3301 cggcagacac ggcgacgagg cctcggccgc ggagcaggcc gaggtacgcc tgatcgcgcg
     3361 cgtggctgcg cgcgagctgc cagccttcga ggagctctac cgctgttatc acccgaggct
     3421 gacgcgcttc ctggatcgcg tgacgcggcg gcccagcctg gtcgaggagc tgctcaacga
     3481 caccatgctg gtggtttgga accgggccga cagctacaac gggcgcagca agctctcgac
     3541 ctggatcttc gccatcgcct accgcaaggc gctgaaggcc ttgcagggac tggacgaagc
     3601 ggtggaagac ccgcaggcgg atcagcgcgc cgcgagcggg ccgggtcccg agcagcaggc
     3661 cgggcaaggc gaattgcggg cggtgctggc cagggcgatg ggcgagcttt cggccgagca
     3721 tcgcgcagtc gtcgacctga cctactttca tggcatcggc tatcgcgaga tcgcgcagat
     3781 cgtcgaatgt ccggtcgaca cggtgaagac gcgcatgttc tatgcccggc ggcggctcag
     3841 ggatctgctc gatggtcaat tgcaggactg gctgccttga ggtgacgaga tgagtgcaag
     3901 agtcgtgaag ctagacgccg acacgcaccg cagcgtgcag gccctgctgc cctggtatgt
     3961 cacgcaaagg ctgggggccg aagacctggc caaggtcgaa acccatctgg ccgcctgccc
     4021 gcattgccag gccgagctgg cctgggagcg caggctgcat gccgccaggc tggaacacga
     4081 cgatttccgc gcccacggcg atgccgaagc cggcttggcg ctgctgcgct cacggatcga
     4141 ggcgagtcgc agcgagcagc caagggcgag actgcgccgc ctgcgcgacg gcgtgcgcgc
     4201 ggccctgcgc gactggcgcc tcgcctggca aggcagcgct g
//
LOCUS       DASUCY010000117         2736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1175159, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000117 DASUCY010000000
VERSION     DASUCY010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1175159"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..680
                     /locus_tag="VFV25_02320"
     CDS             60..680
                     /locus_tag="VFV25_02320"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY1392179.1"
                     /translation="MSEQDTQPTDPDAHLRETSLKSEQAYRGIFLDLRRDEVRLPDGH
                     IAQREYLVHPGAVMVVPLLDDGRVVVERQYRYPLRRAFIEFPAGKLDAGEGGLACAVR
                     ELAEETGYSAREWAQAGVLHNAIGYSNEAIQIWFARGLMPGMRQLDAGEHLDVYSASF
                     EELEAQAASGELTDAKTLIGLLWLGQWRRGLWPLQWQPSPEGSASR"
     gene            691..1152
                     /locus_tag="VFV25_02325"
     CDS             691..1152
                     /locus_tag="VFV25_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HEY1392180.1"
                     /translation="MKVLNLLCSRGHGFEGWFGSEEDYQAQLERGLVECPLCADKGIT
                     RLPSAPRLNLSGLREEPTTPAPVVQPPAAATPEQASVEALWLSAIRHVMAHTEDVGER
                     FAEEARRIHYGESEHRGIRGRTSQEEAVALAEEGIEVMALPIPAALKGPTQ"
     gene            complement(1180..2604)
                     /locus_tag="VFV25_02330"
     CDS             complement(1180..2604)
                     /locus_tag="VFV25_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY1392181.1"
                     /translation="MSDLLVQALRGIVGETQVLQGGDLSAYEVDWRKRYRGKALAVVR
                     PADAAEVAAVVRACAAHGASLVPQGGNTGLVGGSVPDASGRQVLLSLARMNRVREIDA
                     ANLTMTTEAGCVLQAVQEAAAARGLLFPLSLAAEGSCTIGGNLATNAGGTQVLRYGNA
                     RELCLGLEVVTAAGEIWQGLSGLRKDNTGYALRELYIGSEGTLGIITAATLKLYPQPA
                     ARCTALATCETLDDCVALLQLAQARLGAGLTGFELMGRFALGLVDKHFHALPSLPALP
                     PQMKASPWAVLLEQSDTENEAHARKLFSTLLEAALQDGLITDALVAQSLQQSRALWHL
                     REAIPLAQAEEGLNIKHDISLPVSRIAAFVSQTDAALQRAFTGVRLVNFGHLGDGNLH
                     YNVQAPEGIDAAQFLAQHEKAVNTLVYDAVKAHGGSISAEHGIGALKRDELAKRESPV
                     ALELMRAIKRALDPQGIFNPGRLL"
     gene            complement(2601..>2736)
                     /locus_tag="VFV25_02335"
     CDS             complement(2601..>2736)
                     /locus_tag="VFV25_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HEY1392182.1"
                     /translation="TSDPNGRSAALAGLQVLLCLVLFAACALHVRWRLKPAAPREAQA
                     "
BASE COUNT          426 a          938 c          943 g          429 t
ORIGIN      
        1 ctgacgttgg cctttcccgg cttcgtctgg cgctacctgc gtcgtcaccg gccacagcga
       61 tgagcgagca agacacgcag ccgacggatc ccgacgcgca cctgcgggag acgtcgctca
      121 agagcgagca ggcgtatagg ggcatcttcc tggacctgcg acgcgacgag gtgaggctgc
      181 ccgacggtca catcgcccag cgcgaatatc tggtgcatcc cggcgccgtg atggtcgtgc
      241 ccttgctgga cgacggaagg gtggtggtcg agcgccaata ccgctacccg ctgcggcgcg
      301 ccttcatcga gttccccgcc ggcaagctcg acgccggcga gggcggtctc gcctgcgccg
      361 tgcgcgaact ggccgaggag accggctaca gcgcccgcga atgggcgcag gccggcgtgc
      421 tgcataacgc catcggctat tccaacgagg cgatccagat ctggttcgcg cgtggcctca
      481 tgcccggcat gcgccagctc gatgccggtg agcatctcga cgtctacagc gccagcttcg
      541 aggaactgga ggcccaggcg gcgtccggcg aactgaccga tgccaagacc ctgatcggcc
      601 tgctgtggct gggccagtgg cgacgcggac tatggccttt gcagtggcaa ccctcgcctg
      661 agggctctgc cagccgataa tgacggcacg atgaaggttc tcaacctgct atgcagccgc
      721 ggccacggct tcgagggctg gttcggctcc gaggaggact accaggcgca gctggagcgc
      781 ggcctggtgg aatgcccctt gtgcgccgac aagggcatca cgcgcctgcc ttctgcgccg
      841 cgactgaacc tctcaggcct gcgcgaggag ccgacgacgc cagcgcccgt cgtccagccg
      901 ccggccgccg cgacgcccga gcaggccagc gtcgaggcgc tgtggctgag cgcgatacgc
      961 catgtgatgg ctcataccga ggacgtcggc gagcgcttcg ccgaggaggc ccggcgcatc
     1021 cattacggtg agagcgagca tcgcggcatt cgcgggcgca cgagccaaga ggaagccgtt
     1081 gcgctcgcgg aagagggcat cgaagtgatg gccctgccga taccggccgc gctcaagggc
     1141 cccacgcagt aggccctagg cccagccggg cgcggcggtt tagagcaggc gccccggatt
     1201 gaagatgcct tgcgggtcca gcgcccgctt gatggcgcgc atcagctcca aggccacggg
     1261 cgactcgcgc ttcgccagct cgtcgcgctt gagcgccccg atgccgtgct cggccgagat
     1321 cgagccgccg tgggccttta ccgcgtcgta caccagcgta ttgaccgcct tttcgtgctg
     1381 cgccaggaat tgcgcggcat cgatgccctc gggcgcctgc acgttgtagt gcagattgcc
     1441 gtcgcccaga tggccgaagt tgaccagccg cacgccggtg aaggcgcgct gcagcgcagc
     1501 gtcggtctgc gacacgaagg cggcgatgcg cgacaccggc agcgagatgt cgtgcttgat
     1561 gttgagcccc tcctcagcct gcgccagcgg gatagcctcg cgcaagtgcc atagcgcgcg
     1621 cgattgctgc aggctctgcg ccaccagggc atccgtgatc aggccgtctt gcaacgcggc
     1681 ttccagcagg gtggagaaaa gcttccgcgc atgcgcttcg ttctcggtgt cggactgctc
     1741 cagcagcacg gcccaggggc tggccttcat ctgcggcggc agggcgggca ggctgggtag
     1801 cgcatggaag tgcttgtcca ccaggccgag cgcgaagcgc cccatcagct cgaagccggt
     1861 gagcccggcg cccaagcgcg cctgggcgag ctggagcagg gcgacgcagt cgtccagcgt
     1921 ttcgcaggtt gccagcgcag tgcaacgggc cgccggttgg gggtagagct tgagcgtggc
     1981 ggccgtgatg atgcccagcg taccttcgct gccgatgtag agctcgcgca gcgcgtagcc
     2041 ggtgttgtcc ttgcgcagcc cgctcaagcc ctgccagatc tcgccggcag ccgtcacgac
     2101 ttccagcccg aggcagagtt cgcgcgcatt gccgtagcgc agcacctggg tcccgcccgc
     2161 attggtggcg aggttgccgc ctatcgtgca gctgccttcg gccgcgaggc tgagcgggaa
     2221 cagcaggcct cgcgccgcgg ccgcctcctg cacggcctgc agtacgcaac cggcctcggt
     2281 ggtcatcgtc agattggcgg catcgatttc gcgcacccgg ttcatgcgtg cgaggctgag
     2341 cagcacctgc ctgccgctcg catccggcac cgagccgccg accagcccgg tattgccgcc
     2401 ttgcggcacg aggcttgcgc catgcgcggc gcacgccctc acgacagcgg ccacttcggc
     2461 tgcatctgcc gggcgcacca cggccagggc cttgccgcga tagcgcttgc gccagtcgac
     2521 ctcataggca ctgagatcgc cgccctgcag cacctgggtc tcacccacga tgccgcgcag
     2581 ggcttgtact agcaaatcac tcatgcctgc gcttcccgcg gcgcggcggg cttgagtcgc
     2641 cagcgcacat gtagcgcgca ggctgcgaac agtacgaggc acagcagtac ttgcaggccg
     2701 gccagcgcgg cgctgcggcc gttgggatcg ctggtg
//
LOCUS       DASUCY010000118         3185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_982453, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000118 DASUCY010000000
VERSION     DASUCY010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982453"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1134
                     /locus_tag="VFV25_02340"
                     /pseudo
     CDS             1..1134
                     /locus_tag="VFV25_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559640.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            1131..1754
                     /locus_tag="VFV25_02345"
     CDS             1131..1754
                     /locus_tag="VFV25_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY1392183.1"
                     /translation="MSLIPKKGTVYVVDDDEAVRDSLQWLLEGKDYRVKCFDSSESFL
                     SRFDPREVACLIADIRMEGMSGLELQDRLMERKSPLPIVFITGHGDVPMAVSTMKKGA
                     MDFIEKPFKEEELLALVERMLDQARTAFSQHQEQASRDALLSRLTTREAQVLERIVAG
                     RLNKQIADDLGISIKTVEAHRANIMEKLNANTVADLLKIALGAQAKA"
     gene            1816..2682
                     /gene="folD"
                     /locus_tag="VFV25_02350"
     CDS             1816..2682
                     /gene="folD"
                     /locus_tag="VFV25_02350"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEY1392184.1"
                     /translation="MTAQIIDGTALSRQIREEVTHRAAALAARGHRPGLAVILVGEDP
                     ASQVYVRNKVKACQDHGLHSVLEKYEATLSEADLLARITALNRDPSIHGILVQMPLPK
                     HIDAHKVIEAIAPTKDVDGYSVLSAGELMAGLPGFRPCTPYGCMKLIESTGVPIKGKH
                     AVVIGRSNTVGKPMALLLLQANATVTVCHSGTPDLGQHTRQADIVVAAVGRRNTLTAG
                     MVKPGAIVIDVGINRNEEGKLCGDVDFAGVSQVAGWISPVPGGVGPMTITMLLVNTIE
                     SAERTAAEEGQA"
     gene            2679..>3185
                     /locus_tag="VFV25_02355"
     CDS             2679..>3185
                     /locus_tag="VFV25_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HEY1392185.1"
                     /translation="MTTTNPLLDTSGLPRFDAIKPEHVAPAITALLQEADAALEKAVS
                     PAVPADYDALSAVLDVATEKLGRAWGAVGHLNAVADTPELRAAYNENLPKVTDFYTQL
                     GADERLYAKYKALSRSPAVATLNPARQQVLTHAMRDFVLSGAELQGAAKERFQALQEQ
                     QAALAQKFS"
BASE COUNT          599 a         1108 c         1025 g          453 t
ORIGIN      
        1 acgaccgtgc tggaggggct ggacgccgcc gtctcggtgc aatcggtgca gcagcgcgag
       61 ctgctgttcg ccaaccgctc ctaccgcctg tggttcggcg ccgatgcgcg cggccacgcg
      121 ctgctggccg ggatggagca gccgtagcag cagcccgaac gcgacgagtt cgtcgacaac
      181 ctgggcgggc ttccgaccca ggagctgacc gaggccggct ccgacacgcg cgaggtctac
      241 atcgaatcac tgcagaaatg gttcgacgtg cgtgcccgct acctgcagtg gaccgacggc
      301 cggctggcgc agatgctgat cgccaccgac atcacggcgc gccgcagcgc cgaggagcag
      361 gccgcgcgcc aggccgagcg ggcccagatg acgagccggc tgatgaccat gggcgagatg
      421 gcctcctcgg tcgcgcacga gctcaaccag ccgctcaccg ccatcaccaa ctactgcaac
      481 ggcatggtct cgcgcgtgaa ggccggcagc atcaccgagg tcgacctgac cgcggcgctg
      541 gaaaagaccg ccaggcaggc ccagcgcgcc gggcagatca tccaccgcat acgcgccttc
      601 gtgaagcgca gcgagccgca gcgccagccg gcccaggcgc gcgccatcgt cgaggatgcg
      661 gtggagctgg ccggcatcga gctgcggcgg cgcaacgtca acatccacac ctatgtggcc
      721 cagcgcctgc ccagcatcat ggtcgatccc atcctgatcg aacaagtggt gatgaacctg
      781 ctgaagaatt cggccgaggc gatcgacaac gcccagctgc cgcccgcgcg gcgcaacatc
      841 gagctgcgcg tggtgcccaa gtacacgtcg gacgaaggcg gcgtgattga attcagcgtc
      901 agcgattcgg gccccggcat caaggacgag gtgatcagcc ggctctacga ggccttcttc
      961 tcgaccaagg ccgacggcct gggcatcggc ctgagcctgt gccgctccat catcgagtcg
     1021 caccgcggcc ggatcaaggc ggagaacctc tacaatggtt ccaccgtggc cggctgccgt
     1081 ttcaccttca cgctgccagt cgaaatcacc cactcccacg tcgaaactgc atgagcctga
     1141 tcccgaagaa aggcacggtc tatgtcgtcg atgacgacga ggccgttcgc gattccctcc
     1201 agtggctgct cgaaggcaag gactacaggg tcaagtgctt cgactcctcc gagtccttcc
     1261 tctcccgctt cgatccgcgc gaagtcgcct gcctgatcgc cgacatccgc atggaaggca
     1321 tgagcggcct ggagctgcag gaccggctga tggagcgcaa gtcgccgctg cccatcgtct
     1381 tcatcaccgg ccatggcgac gtgccgatgg ccgtgagcac gatgaagaag ggcgcgatgg
     1441 acttcatcga gaagcccttc aaggaagaag agctgctcgc cctggtggag cgcatgctcg
     1501 accaggcgcg taccgccttc tcgcagcacc aggaacaggc cagccgcgac gcgctgctgt
     1561 cgcgcctgac tacgcgcgag gcccaggtgc tggagcgcat cgtcgccggc cggctgaaca
     1621 agcagatcgc cgacgacctt ggcatcagca tcaagacggt ggaggcgcat cgcgccaaca
     1681 tcatggaaaa gctcaacgcc aacaccgtgg ccgatctgct gaagatcgca ctcggcgcgc
     1741 aggccaaggc ctgagacctc acacgacgac aagggccgct gcacagcggc ctttgtcatt
     1801 tgcaggacga cacacatgac cgcacagatc atcgacggca ccgccctctc tcgccagata
     1861 cgcgaggaag tcacgcaccg cgccgcggcc ctcgccgcgc gcggccaccg gcccggcctg
     1921 gcggtgatcc tggtgggcga ggatcccgcc agccaggtct atgtgcgcaa caaggtcaag
     1981 gcctgccagg accatggcct gcattcagtg ctcgagaagt acgaggcgac gctgtccgag
     2041 gccgacctgc tggcgcgcat aactgcgctg aaccgcgatc ccagcatcca cggcatcctg
     2101 gtgcagatgc cgctgcccaa gcacatcgac gcgcacaagg tgatcgaggc gatcgcgccg
     2161 accaaggacg tggacggcta ctcggtgctg agcgccggcg agctgatggc cgggctgccg
     2221 ggctttcgcc cctgcacgcc ctatggctgc atgaagctga tcgaaagcac cggcgtgccc
     2281 atcaagggca agcatgcggt cgtgatcggc cgcagcaaca cggtgggcaa gcccatggcc
     2341 ctgctgctcc tgcaggccaa tgccaccgtc accgtgtgcc acagcggcac gcccgatctc
     2401 ggccagcaca cgcgccaggc cgacatcgtg gtggccgccg tcggccggcg caacacgcta
     2461 acggccggga tggtcaagcc cggcgccatc gtgatcgacg tgggcatcaa ccgcaacgag
     2521 gaaggcaagc tctgcggcga cgtggacttt gccggtgtct cccaggtcgc aggctggatc
     2581 tcgccggtac cgggcggcgt cggtcccatg accatcacca tgctgctcgt caataccatc
     2641 gagtctgctg agcgcaccgc cgccgaggaa ggacaagcat gaccacgacc aaccctttgc
     2701 tcgacacgag cggcctgcct cgtttcgatg cgatcaagcc cgagcatgtg gcgccggcca
     2761 tcacggcgct gctgcaggaa gccgatgcgg cgctggagaa ggcggtgagc cccgccgtgc
     2821 cggcggacta tgacgcgctg tccgccgtgc tggacgtggc gaccgagaag ctgggccgcg
     2881 cctggggcgc ggtcggccat ctcaacgccg tggccgacac gcccgagctg cgcgccgcct
     2941 acaacgagaa cctgcccaag gtcaccgact tctataccca gttgggcgcc gacgagcgcc
     3001 tctacgccaa gtacaaggcc ctgagccgga gccctgcggt cgcgacgctg aacccggcgc
     3061 gccagcaggt gctgacccat gcgatgcgcg acttcgtgct ctcgggcgcc gagctgcagg
     3121 gcgccgccaa ggaacgcttc caggcgctgc aggaacagca ggccgcactc gcgcagaaat
     3181 tctcc
//
LOCUS       DASUCY010000119         2684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1062932, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000119 DASUCY010000000
VERSION     DASUCY010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062932"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..1013
                     /locus_tag="VFV25_02360"
     CDS             168..1013
                     /locus_tag="VFV25_02360"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEY1392186.1"
                     /translation="MPTPLIDVASLQALLGCDAPPLLLDASFDLADPEAGRRAHEQGH
                     LPGALYVHLDRDLSAPKTGCNGRHPLPSREDFTVRVGAWGLTPERAVVVYDAQGGVYA
                     ARTWWMLRWLGHESVAVLDGGLQAWTAAGGTLTSVASSPRPAPPYPLRAAATMPALDA
                     DSLQRSLGRVRLIDARAPERFRGDVEPLDPVAGHIPGALNRLFRLNLAADGRFKPAAQ
                     LRAEFMPLLGGLAPQDVVHQCGSGVTACHNLLAMEAAGLGGAALYPGSWSEWCADPAR
                     PLARG"
     gene            1169..1603
                     /locus_tag="VFV25_02365"
     CDS             1169..1603
                     /locus_tag="VFV25_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY1392187.1"
                     /translation="MYKRILVPTDGSDITAKAVDTAIALARSLSAHVCTLAVKEPFPY
                     SAISEMQPVPPQEFYDTQNALANRHLQAVKDACAAAGIACDAQSVEALHPWEAIIEHA
                     KAQGCDLIVMASHGRRGMSALLLGSETQKVLTHTKTPVLIVR"
     gene            complement(1661..2431)
                     /locus_tag="VFV25_02370"
     CDS             complement(1661..2431)
                     /locus_tag="VFV25_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEY1392188.1"
                     /translation="MLRVLIVDDEELARSRLSALVSEVREPLCQVVGEAANAAQALDW
                     LASSHGGGADVLLLDVQMPGLAGTELAARLSSLARPPAVIFVTAHAEHALRAFELDAV
                     DYLTKPVRRERLHAALQRAAQRRGSQPAAQAPADRPTLVVSDRGRLARVPLAEVLYLK
                     AELKYVTLRTASHTYVLDDALADLERRLGPGFLRVHRNALVARRAVRQLERRHLDGQG
                     EDEGSEAWAVCVAPVDEWLAVSRRQVAAVREALADEGL"
     gene            complement(2459..>2684)
                     /locus_tag="VFV25_02375"
     CDS             complement(2459..>2684)
                     /locus_tag="VFV25_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY1392189.1"
                     /translation="PSAEGGAIRVRTRLRRGHAIVSVSNSLPGLPSQPGRGMALRNVR
                     ERLRLMHDVAAQFEAGAHGDVYRVQIVVPV"
BASE COUNT          420 a          966 c          894 g          404 t
ORIGIN      
        1 gccttctcct tcctctatgg cgtcttcctg ttccatgatc cggtcacggc catggccgtg
       61 ggcggcatgg tcatcgtcgt gatcgccggc attgcggccg cgcggctgcg ccaggcgagc
      121 gccccgcaag tcagcgcagc gcccaactcc accctcgaaa catgaaattg cccacgcccc
      181 tgatcgacgt cgccagcctg caggccctgc tgggctgcga tgcaccgccg ctgctgctcg
      241 atgcaagctt cgacctggcc gaccccgagg cgggacggcg cgcccacgag caaggccatc
      301 tgcccggcgc gctctatgtg catctggacc gcgatctctc cgcgcccaag accggctgca
      361 acggccgcca cccgctgccc agccgcgagg acttcaccgt gcgcgtcggc gcctggggcc
      421 tcacgcccga gcgcgcggtc gtggtctacg acgcccaggg cggcgtctat gccgcgcgca
      481 cctggtggat gctgcgctgg ctgggccacg agagcgtcgc ggtgctggac ggcggcctgc
      541 aggcgtggac ggcggcaggc ggcacgctga cgagcgttgc atcgagtccc cgccccgcgc
      601 cgccctaccc attgcgggcg gcggcaacga tgcctgcgct ggacgccgac agcctgcagc
      661 gctcgctggg gcgggttagg ctgatcgatg cccgggcgcc ggagcgcttt cgcggcgacg
      721 tcgagccgct ggacccggtg gctgggcata ttccgggcgc gctgaaccgg ctcttcaggc
      781 tcaatctggc ggcagacggc cgcttcaaac ccgctgcgca gctgcgcgcc gagttcatgc
      841 cgctgctggg cggccttgca ccccaggacg tggtgcatca atgcggctcg ggcgtcacgg
      901 cctgccacaa cctgctggcg atggaggcgg cgggactggg cggcgcggcg ctctatccgg
      961 gttcgtggag cgaatggtgc gccgacccgg ccaggcccct cgctcgcggg tagggaatgc
     1021 tacaaatatt gatgcgcaga tccccaaaat aaaacggatg ttagactgat ttttctgtat
     1081 ttttgaccaa aatcaagctc cacaagcgct cttgtccgta gcatcatcaa cgagagcgca
     1141 ttccgcgcct cagctgaagg agatgagcat gtacaagcgc atcctcgttc ccaccgacgg
     1201 ctcggacatt accgccaagg cagtcgacac ggctattgca ctggcgcgca gcctgtcggc
     1261 ccacgtctgc acgctggccg tgaaggagcc cttcccctac agcgcgatct cggaaatgca
     1321 gccggtgccg ccccaggagt tctacgacac gcagaacgcc ctggccaacc gccacctgca
     1381 ggccgtgaaa gacgcctgtg ccgcggccgg tattgcctgc gacgcgcaaa gcgtcgaggc
     1441 cctgcacccc tgggaagcca tcatcgaaca tgccaaggcc caaggctgcg acctgatcgt
     1501 gatggcctcg cacggccggc gcggcatgag cgcgctgctg ctgggcagcg agacgcagaa
     1561 ggtgctgacg cacacgaaga cgccggtgct gatcgtcaga tagtgactgg tgaccagtga
     1621 gtggtgacta gtcactgatc acaggtcaca agtcactact tcagaggcct tcgtccgcca
     1681 gcgcttcgcg caccgcggcc acctggcggc gcgagacggc cagccattcg tccaccgggg
     1741 cgacgcagac ggcccaggct tcgctgcctt cgtcctcgcc ctggccgtcg agatggcggc
     1801 gctcgagctg gcgcacggcg cggcgcgcca ccagggcatt gcggtgcacg cgcaggaagc
     1861 cgggccccag gcgacgttcc agatcggcca gcgcgtcgtc gagcacatag gtgtggctcg
     1921 cggtgcgcag cgtcacgtat ttcagctcgg ccttcagata cagcacctcg gccagcggca
     1981 cccgcgccag ccggccgcgg tcgctcacca ccagcgtcgg ccggtcggcc ggcgcctggg
     2041 ccgcgggctg ggagccgcgt cgctgggccg cgcgctgcag cgcggcatgc aggcgctcgc
     2101 gccgcaccgg cttggtcagg tagtcgacgg catccagctc gaaggcgcgc agcgcatgtt
     2161 cggcatgggc cgtgacgaag atcaccgcag gcggccgcgc caggctgctc agccgggcgg
     2221 ctagctcggt gccggccagg ccgggcatct gcacgtcgag cagcagcaca tcggcaccgc
     2281 cgccatgcga agacgccagc cagtcgagcg cctgcgcggc attggcggcc tcgccaacca
     2341 cctggcacag tggttcgcgc acctcgctca ccagcgcgct caggcgcgag cgggccagct
     2401 cctcatcgtc gacgatcagc acgcgcagca tgctccccct cccttctttg actgtgagct
     2461 ataccggcac gacgatctgc acccggtaga catcgccatg cgcgccggcc tcgaactgtg
     2521 ccgcgacatc gtgcatcagg cgcaggcgct cgcgcacatt gcgcaaggcc atgccgcggc
     2581 ccggctgcga gggcaggccc ggcaggctgt tgctcaccga cacgatggca tggccgcgcc
     2641 gcagccgcgt gcgcacgcgt atcgccccgc cctcggcgct gggc
//
LOCUS       DASUCY010000120         2896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_179399, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000120 DASUCY010000000
VERSION     DASUCY010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179399"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="VFV25_02380"
     CDS             complement(<1..803)
                     /locus_tag="VFV25_02380"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEY1392190.1"
                     /translation="MGSSASSVGVVAPQTLHFAQALALRSGAQLADYTLSYETYGELN
                     ADKSNAVLVCHALNASHHVAGVHAGVDKSEGWWDNLVGPGKPLDTNRFFVIGVNNPGS
                     CFGSTGPMQTNPATGRPYGADFPVVTVEDWVDAQARLLDALGIAQLAAVIGGSLGGMQ
                     ALSWTLQYPERVRHALVIASAPNLSAQNIAFNEVARRAIVTDPDFHDGHYYGHGVLPR
                     RGLRVARMIGHITYLSDDVMEQKFGRELRAAELAYSTQDIEFQIESYLR"
     regulatory      complement(855..930)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            1002..2648
                     /locus_tag="VFV25_02385"
     CDS             1002..2648
                     /locus_tag="VFV25_02385"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HEY1392191.1"
                     /translation="MPLPPWLRKLDQLYPVRYTAWLLCTVFFLLSALVWVAVGQAVLA
                     TLVFGTLVLVGLHDVRQHRHSVLRNYPVIGHLRFMLEYIRPELRQYFIEGDNDAAPFS
                     RTQRSLVYQRAKGVNDKRPYGTQLDVGLPGYEWINHSIAPTRLPTHDFRITIGPERAL
                     PYSASVFNISAMSFGALSANAIRALNTGARRGGFAHDTGEGSISRYHRENGGDLIWEV
                     GSGYFGCRNEDGSFSAERFAEAARDPQVKMIELKLSQGAKPGHGGVLPGAKVTPEIAA
                     ARGVPAGVDCISPARHSAFDSPIGMMQFIERLRELSGGKPTGFKLCIGHPWEWFAIAK
                     AMLATGITPDFIVVDGAEGGTGAAPLEFTDHVGAPLQEGLLLVHNTLVGIGLRERIRI
                     GCAGKVVSAFDIARMMALGADWCNSARGFMFALGCIQSQTCHTGRCPTGVTTQDTLRQ
                     SALVVPDKAERVFQFHETTLEALRELVQAAGLQHPGEIKAAHIVRRTAEHEVKLLASL
                     LPFAKPGALLAAERGEGGWPHRVFELYWPIARAESFAPAA"
     gene            complement(2672..>2896)
                     /locus_tag="VFV25_02390"
     CDS             complement(2672..>2896)
                     /locus_tag="VFV25_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HEY1392192.1"
                     /translation="RELAPTLPLLIPGVGAQGGDAAATVRAGWREDGPIIVNSSRAVL
                     YAGAGEDFASAARAVARATRDALNAAKLAR"
BASE COUNT          460 a         1010 c          958 g          468 t
ORIGIN      
        1 tagcgcagat agctctcgat ctggaactcg atgtcctggg tgctgtaggc cagctcggcg
       61 gcgcgcagct cgcggccgaa cttctgctcc atcacatcgt cgctcagata ggtgatgtgg
      121 ccgatcatgc gcgccacgcg cagcccgcgc ctgggcaaga cgccatgtcc atagtagtga
      181 ccgtcgtgga aatcggggtc ggtcacgatg gcgcggcgcg ccacctcgtt gaaggcgatg
      241 ttctgcgccg acagattggg ggcgctggcg atcaccagcg catggcgcac gcgctcgggg
      301 tattgcagcg tccagctcaa ggcctgcatg ccgcccaggc tgccgccgat caccgccgcc
      361 agctgcgcta tgcccagcgc atcgagcagc ctggcctgcg cgtcgaccca gtcctccacc
      421 gtcacgaccg ggaagtccgc gccatagggc cgcccggtcg cgggattggt ttgcatcggc
      481 ccggtggagc cgaagcagga gccggggttg ttgacgccga tgacgaagaa gcggttcgtg
      541 tccagcggct tgcccgggcc gaccaggttg tcccaccagc cttcgctctt gtccacgcca
      601 gcgtggacgc ccgccacatg gtgcgaggca ttcagcgcat ggcagaccag cacggcattg
      661 ctcttgtcgg cgttgagctc gccataggtc tcgtagctca gcgtgtagtc ggccagctgc
      721 gcgccgctgc gcagcgccag tgcttgagcg aaatgcaggg tttgcggcgc caccacgccg
      781 accgaactag ccgaggagcc catgaataat gaacggcaga aacgaaaaac ccggcctcac
      841 gctgcagtga gccgggttcg cttcgccggg cccctttagc tggatttctt gagcgcccgc
      901 aagccggtac aaatcggcgc tgggcggcat tgtaacgaag cggcacgcgg ggcggtcgct
      961 gggctgctat cgttgccgcc ccgtttgaga ggagctctcc catgcccttg cctccttggc
     1021 tgcgcaagct cgaccagctc tatccggtcc gctacacggc ctggctgctg tgcacggtct
     1081 tcttcctact cagcgcgctg gtctgggtgg ccgtgggcca ggcggtgctg gccacgctgg
     1141 tcttcggcac gctggtgctc gtgggcctgc acgacgtgcg ccagcaccgc cactcggtac
     1201 tgcgcaacta ccccgtcatc ggccacctgc gcttcatgct cgagtacatc cgccccgagc
     1261 tgaggcagta tttcatcgag ggcgacaacg acgccgcgcc cttctcgcgc acccagcgct
     1321 cgctggtcta ccagcgcgcc aagggcgtca acgacaagcg gccctatggc acccagctcg
     1381 atgtcggcct cccgggctac gaatggatca accactcaat cgcgccgacg cggctgccca
     1441 cgcacgactt ccgcatcacc atcggccccg agcgcgcgct gccctacagc gccagcgtct
     1501 tcaacatctc ggcgatgagc ttcggcgcct tgagcgccaa tgcgatacgc gcgctcaaca
     1561 ccggcgccag gcgcggcggc ttcgcccacg acaccggcga gggcagcatc tcgcgctatc
     1621 accgcgaaaa cggcggcgac ctgatctggg aggttggctc gggctacttc ggctgccgca
     1681 acgaggacgg cagcttcagc gccgagcgtt ttgccgaggc tgcgcgcgac ccacaggtaa
     1741 agatgatcga gctcaaactg agccagggcg ccaagcccgg ccatggcggc gtgctgccgg
     1801 gcgccaaggt cacgcccgag atcgccgcgg cgcgcggcgt gccggccggc gtggactgca
     1861 tctcgccagc gcgccacagt gccttcgact cgcccatcgg catgatgcag ttcatcgagc
     1921 gcctgcgcga gctctcgggc ggaaagccca ccggcttcaa gctgtgcatc ggccatccct
     1981 gggaatggtt cgcgatcgcc aaggccatgc tcgccaccgg catcacgccg gacttcatcg
     2041 tcgtcgatgg agccgagggc ggcaccgggg ccgcgccgct ggagttcacc gaccatgtcg
     2101 gcgcgccgct gcaggaaggg ttgctgctgg tgcacaacac cctggtcggc atcggcctgc
     2161 gcgaacgcat ccgcatcggc tgcgccggca aggtggtcag cgccttcgac atcgcgcgca
     2221 tgatggccct gggcgcggac tggtgcaatt cggcgcgcgg cttcatgttc gcgctgggct
     2281 gcatccagtc ccagacctgc cacaccggcc gctgcccgac cggcgtcacg acgcaggaca
     2341 ccctgcgcca gagcgccctg gtggtgccgg acaaggccga gcgcgtgttc cagttccacg
     2401 aaacgacgct ggaggccctg agggagctgg tgcaggccgc gggcctgcag catccgggtg
     2461 agatcaaggc ggcccatatc gtgcggcgca ccgccgagca tgaggtgaag ctgcttgcga
     2521 gcttgctgcc tttcgcgaag ccgggcgccc ttttggcggc cgagcgcggc gagggcggct
     2581 ggccgcatcg ggtgtttgag ctttattggc ctatcgcgcg tgcagagagc tttgcgccag
     2641 cggcctgagg tagtggcgct agtagttgct gtcagcgtgc caacttggcc gcgttcagcg
     2701 catcgcgcgt ggcacgcgca accgcgcgcg cagcagacgc gaagtcctcg cccgcccccg
     2761 catacagcac cgcccgcgaa gaattgacga tgatgggccc atcctcgcgc caccccgcgc
     2821 gcaccgtggc cgcagcatcg ccgccctggg caccgacgcc cggaatcagc agcggcaagg
     2881 tcggcgcgag ctcgcg
//
LOCUS       DASUCY010000121         2110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_340032, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000121 DASUCY010000000
VERSION     DASUCY010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340032"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VFV25_02395"
     CDS             <1..262
                     /locus_tag="VFV25_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY1392193.1"
                     /translation="RGAPHEAGIWTFEDLSSRRPLTTPGRAGLTGREREVAALVIEGQ
                     TSKQIGRALGLSHRTVDIYRARLLRKYGAGTTAELVQRLVSG"
     gene            327..1031
                     /locus_tag="VFV25_02400"
     CDS             327..1031
                     /locus_tag="VFV25_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEY1392194.1"
                     /translation="MWHDFALRVLLALVLGAVIGTERQLRQRMAGLRTNALVAAGAAL
                     FVAVSALSGDAQGEARIAAQVVSGIGFLGAGVIMREGLNVRGLNTAATLWCSAAVGVL
                     SGLGYALEAAIGTFFVLGANFLLRNVAQRLNRQVPSAAVEVEQAYRITAVCAASEEIH
                     VRSLLLHMLNGMPALILQSLHSEDVPTAAGKIEVRADLLAAPGNQSLLEQLVSRVSLE
                     KSVSVVRWSVLSESLE"
     gene            1143..>2110
                     /gene="lplT"
                     /locus_tag="VFV25_02405"
     CDS             1143..>2110
                     /gene="lplT"
                     /locus_tag="VFV25_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HEY1392195.1"
                     /translation="MPLSFYLLIAAQFVSGLADSALLIVSIARLGEMGSAHWQVPLLK
                     LCFTIAYVVLAPFAGPLADAWPKGRVMLATNGIKAAGCLLIAAGGDPLIGFVVVGLGA
                     TGYSPAKYGLITEIVPPQRLVEANGWIEVSTVGAIILGTVLGGALVGPLADAAWLLWA
                     PPAAMQAAARLLLPLLLVLALYALAAALNLCLPDSGARYAAASRRPLALLAEFRRDNA
                     ALWRDPEARISLAVTTLFWGVGATLQFLVLRWATEALDLDLHQAAYLQGVTAIGITAG
                     AALAGRCVPLGRAGTVLPLGVAMGLLVPLMTATGSVATALPLLILVG"
BASE COUNT          277 a          743 c          750 g          340 t
ORIGIN      
        1 ccgaggcgcg ccgcacgagg ccggcatctg gaccttcgag gacctgtcct cgcgccgccc
       61 gctgaccacg ccgggccgcg ccggcctgac cggccgcgag cgcgaggtgg cggccctggt
      121 gatcgaaggc cagaccagca agcagatcgg cagggccctg ggcctgagcc accgcacggt
      181 ggacatctac cgcgcgcggc tgctgcgcaa gtacggcgcc ggcaccacgg cggagctggt
      241 gcaaaggctc gtctccggct gaacgggccg tctatgcggc acactgcagc gccgccactc
      301 gagtcaatgc ggaggaggga aaaatgatct ggcacgactt cgccctcagg gtgctattgg
      361 cgctcgtact gggcgccgtg atcggcaccg aacgccagct gcgccaacgc atggcggggc
      421 tgcgcaccaa tgcgctggtg gccgcaggcg cggcgctctt cgtcgcggtg tcggccctgt
      481 ccggcgacgc ccagggcgag gcgcgcatcg cggcacaagt ggtctcgggc atcggcttcc
      541 tgggcgccgg cgtcatcatg cgcgagggct tgaacgtgcg cggcctgaac acagcagcaa
      601 cactgtggtg ctcggccgcg gtgggcgtgc tgtccggcct gggctatgcg ctggaagcgg
      661 cgatcggcac cttcttcgtg ctgggcgcaa acttcctgct gcgcaacgtc gcgcagcggc
      721 tgaaccgcca ggtgccgtcc gcggcggtgg aggtcgagca ggcctaccgc atcacggccg
      781 tgtgcgccgc cagcgaagag attcatgtgc gcagcctgct gctgcacatg ctcaacggca
      841 tgcctgcgct gatcctgcaa tcgctgcaca gcgaggacgt gcccaccgcg gccggcaaga
      901 tcgaggtgag agccgatctg ctggccgcgc ccggcaacca gagcctgctg gagcagctcg
      961 tcagccgggt cagcctcgaa aagagcgttt cggtggtgcg ctggtcggtg ctgagcgagt
     1021 ccttggagta gcgaggcgct gtcgcgccca agccgcacgg atgccgaaag cttgcgcgct
     1081 gtcacgcggc ttaaatcttc gtgtcatcaa atcgcggccg ttgcttccag gcgtcccgcg
     1141 tcttgcccct cagcttctac ctgctcatcg ccgcgcaatt cgtctcgggc ctggccgaca
     1201 gcgccctgct catcgtctcc attgcacggc tcggcgagat gggcagcgcc cactggcagg
     1261 tgccgctgct caagctgtgc ttcacgatcg cctatgtcgt gcttgcgccc ttcgccgggc
     1321 cgctggccga tgcctggccc aagggccggg tgatgctggc gaccaatggc atcaaggcgg
     1381 ccggctgcct gctgatcgcg gccggcggtg atccgctgat cggcttcgtc gtcgtcggcc
     1441 tcggcgcgac tggctactcg ccggccaagt acggcctcat caccgagatc gtgccgccgc
     1501 agcgcctggt ggaggccaac ggctggatcg aggtcagcac ggtgggcgcg atcattctcg
     1561 gcaccgtgct aggcggcgca ctggtggggc cgctcgccga tgccgcctgg ctgctgtggg
     1621 ctccgccggc cgcgatgcag gccgcagcgc gactgctgct gcctctgctg ctggtgctgg
     1681 cgctgtacgc actggccgcg gccttgaacc tctgcctgcc ggacagcggc gcgcgctatg
     1741 ccgcggccag ccgccgcccg ctcgcgctgc tggccgagtt ccggcgcgac aacgccgcgc
     1801 tgtggcgcga cccggaagcc cgcatctcgc tcgcggtgac cacgctgttc tggggcgtgg
     1861 gcgccacgct gcagttcctg gtgctgcgct gggccaccga ggccctggac ctcgacctgc
     1921 atcaggccgc ctacctgcaa ggcgtcacgg ccatcggcat cacggccggc gccgcgctgg
     1981 cgggccgctg cgtgccgctc gggcgcgccg gcacggtgct gccactgggc gtcgcgatgg
     2041 gcctgctggt gccgctgatg acggcgaccg gcagcgtcgc cacggccctg cccctgctga
     2101 tcttggtggg
//
LOCUS       DASUCY010000122         2049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_131118, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000122 DASUCY010000000
VERSION     DASUCY010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131118"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1317)
                     /locus_tag="VFV25_02410"
     CDS             complement(<1..1317)
                     /locus_tag="VFV25_02410"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY1392196.1"
                     /translation="MDAAAEAQSPSSPLYQLIGGQIERAIESGALRVGDRLPSVRQLS
                     QQHGVSMSTALQALRWLENRGVVQARPKSGYYVAGRSPTLPEPQLDLRMEGASFVNMD
                     QVLQEFLYIVDDPLALPRFHAAPARSLLPEAKLQHLTASVNRRHPEYASGYSMTGSAA
                     LRHEIARRAVNSGVQLRPDEIVVTNGGLEAVYLALRSVAASGDTIVMESPTYFHLLQL
                     NESLGMKVLEIPSHPRTGLSLEALDFATQTPGAVKACILLPNFPNPTGSLMPVENKRR
                     LVAMMAERGITIIESDIYGELHFGEQRPPVLKSFDATGDVILCSAFTKTLAPGYRVGW
                     VAPGRHFLKTQALKFRTSVACPMLQQEVLAQFIRDGGYEHHLRKLRAALRSQAEHMAD
                     AVARYFPMGCRLSMPQGGLLMWIELPRHVDSREVFALARLEHIGVAP"
     gene            1491..1835
                     /locus_tag="VFV25_02415"
     CDS             1491..1835
                     /locus_tag="VFV25_02415"
                     /inference="COORDINATES: protein motif:HMM:NF022592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2917 domain-containing protein"
                     /protein_id="HEY1392197.1"
                     /translation="MASWLKFQGPVVVQLQDGELLPVESVWLSVVRGRVWLTQAGDPD
                     DHFIDSGQAIRLAAGCRALMSAEGPAQVSLMPAPSWLRALLEKASQWSLLSRRRRDGR
                     RPARAWAPSPGH"
     gene            1876..>2049
                     /locus_tag="VFV25_02420"
     CDS             1876..>2049
                     /locus_tag="VFV25_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEY1392198.1"
                     /translation="MHAPKIPEHEVEITAIRAQGAGGQNVNKVSSAIHLRFDIAASSL
                     PEAVKARLLALNDQ"
BASE COUNT          321 a          667 c          728 g          333 t
ORIGIN      
        1 cggcgccacg ccgatgtgct ccagccgcgc cagcgcgaac acctcgcgcg aatcgacatg
       61 gcgcggcagc tcgatccaca tcagcaggcc gccttgcggc atcgagagcc ggcaacccat
      121 cgggaaatag cgcgccaccg cgtctgccat gtgctcggcc tgcgatctca gcgcggcgcg
      181 cagcttgcgc agatgatgct cgtagccgcc gtcgcggatg aactgcgcca gcacttcctg
      241 ctgcagcatg gggcaagcca ccgaggtgcg gaacttcagc gcctgcgtct tcaggaaatg
      301 cctgcccggc gcgacccagc cgacgcggta gccgggtgcc agcgtcttgg tgaaggccga
      361 gcacaggatc acgtcgccgg tggcatcgaa gctcttgagc accggcgggc gctgctcgcc
      421 gaagtgtagc tcgccgtaga tgtcgctctc gatgatggtg atgccgcgct cggccatcat
      481 cgcgaccagg cggcgcttgt tttccaccgg catcaggctg cccgtcgggt tggggaagtt
      541 gggcagcagg atgcaggcct tcaccgcgcc aggcgtctgc gtcgcgaagt cgagtgcctc
      601 gagcgacagg ccggtgcgcg ggtggctggg aatctccagc accttcatgc ccaggctctc
      661 gttgagctga agcaggtgaa agtaggtagg cgactccatc acgatggtgt cgcccgaggc
      721 ggccaccgag cgcagcgcca gatacaccgc ctccagcccg ccgttggtga cgacgatttc
      781 gtcggggcgc agctgcacgc cggagttcac cgcgcgacgc gcgatctcgt ggcgcagcgc
      841 ggcgctgccg gtcatgctgt agcccgaggc gtactccggg tggcggcggt tcacgctggc
      901 cgtcaggtgc tgcagcttgg cctcgggcag cagcgaacgc gccggcgcgg catggaagcg
      961 cggcagggcc agcggatcgt cgacgatgta caggaactcc tgcagcacct ggtccatgtt
     1021 gacgaaggag gcgccttcca tgcgcagatc cagctgcggc tcgggcagcg tgggcgagcg
     1081 gcccgcgacg tagtagccgg acttgggccg cgcctgcacg acgccccggt tctccagcca
     1141 gcgcaaggcc tgcagcgcgg tgctcatgct cacgccgtgc tgctggctca gctggcgcac
     1201 cgagggcagg cggtcgccca cgcgcagtgc gccgctctcg atggcgcgct cgatctgccc
     1261 gccgatgagc tgatacagcg gcgacgacgg tgactgcgct tcggcggcgg cgtccatgat
     1321 gactccattg tgacgagctt cggtgctgct gtaccagaac agatgtggat ctcaatggcc
     1381 tatacagagg cctgtgcagc ccgtctgttc cggtgcagtt catcggatgc tgggtctgtt
     1441 ggcgaaaggc gccgatgccg acactttcgg ttcatgagaa ggagtcggcg atggcatcgt
     1501 ggttgaagtt ccaaggtccg gtggtggtgc agttgcagga tggcgagctg ctgccggtgg
     1561 agtccgtttg gctgagcgtg gtgcgcgggc gcgtgtggct tacgcaagcc ggtgatccgg
     1621 acgaccattt catcgacagc gggcaggcca tccgcctagc ggcgggctgc agggcgctga
     1681 tgagcgccga agggccggcg caggtgagcc tgatgccggc accgtcgtgg ctgcgggccc
     1741 tgctggagaa ggcctcacaa tggtccttgt tgtcgcgtcg ccgccgcgac gggcgccggc
     1801 ccgcgcgtgc ctgggcgccg tctcccgggc attgagccgg cggcatgcga cggacgagag
     1861 cagaccgccg cagccatgca cgcccccaag attcccgagc acgaggttga gatcaccgcg
     1921 atccgcgccc agggcgcggg cgggcagaac gtcaacaagg tgtccagcgc gatccatctg
     1981 cgcttcgaca tcgccgcctc gtcgctgccc gaagccgtca aggcgcggct gctggccctg
     2041 aacgatcag
//
LOCUS       DASUCY010000123         2839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_50802, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000123 DASUCY010000000
VERSION     DASUCY010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50802"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /gene="glyA"
                     /locus_tag="VFV25_02425"
     CDS             <1..425
                     /gene="glyA"
                     /locus_tag="VFV25_02425"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904615.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEY1392199.1"
                     /translation="EALGPEFKAYQQQVVKNAAVLADTLTQRGLRIVSGRTESHVMLV
                     DLRPKGLTGKEAEAILGQAHMTCNKNGIPNDPQKPMVTSGIRLGSPAFTTRGFKEAEA
                     ALTANLIADVLDKPHDEANIAAVRAKVAALTRDFPVYR"
     gene            471..914
                     /gene="nrdR"
                     /locus_tag="VFV25_02430"
     CDS             471..914
                     /gene="nrdR"
                     /locus_tag="VFV25_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEY1392200.1"
                     /translation="MRCPFCGHEDTQVVETRESDEGDVIRRRRRCPACDKRFTTYERP
                     ELAMPAIVKKDGSRSEFDRNKIRASMLLALRKRPVSIDQVDSALARIEEKLLSSGANE
                     VPSARIGEMVMRELKKLDKVAYVRFASVYRSFEGVDEFSRLIKDI"
     gene            1223..2344
                     /gene="ribD"
                     /locus_tag="VFV25_02435"
     CDS             1223..2344
                     /gene="ribD"
                     /locus_tag="VFV25_02435"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEY1392201.1"
                     /translation="MPHTFSPRDEELMRLALAEAQQALPLSPPNPAVGCVIADAEGQI
                     LGQGHTQLAGGPHAEVMALRAARTRRPDLAGCTAYVTLEPCSHHGRTPPCVNALIEHS
                     IARVVAAVQDPNPLVAGRGLAMLREAGVQVELGLLGEQARELNIGFFSRMVRRRPWLR
                     MKVAASLDGVTALDNGASQWITSEAARTDGHRWRARAGAVLTGVGTVREDDPRLDVRL
                     VPTQRQPIRVIVDSRLETPRQAHILAPPGEVLIYCASATRQDAAEFQALGAQLIALPN
                     QAGKVELAAMLKDLAQRGINELHVEAGHRLNGSLLREGLVDEFLIYLAPKLLGQGRSM
                     ASFGPLHRLQDAVELEFLDVEHVGPDLRIVARPPGRATF"
     gene            2405..>2839
                     /locus_tag="VFV25_02440"
     CDS             2405..>2839
                     /locus_tag="VFV25_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEY1392202.1"
                     /translation="MAISPVPELIAELAAGRMVILVDEEDRENEGDLVLAADHVTPEA
                     INFMAKYGRGLICLTLTRERCERLQLPPMTQRNGAQHGTAFTVSIEAAEGVTTGISAA
                     DRAHTVKVAVARECRARDLVQPGHVFPLQAQDGGVLMRAGHTE"
BASE COUNT          480 a         1005 c          936 g          418 t
ORIGIN      
        1 aggaggcgct cggccccgag ttcaaggcct accagcagca ggtggtgaag aacgccgcag
       61 tgctggccga cacgctgaca cagcgcggcc tgcgcatcgt cagcggccgc accgagagcc
      121 atgtgatgct ggtcgacttg cgccccaagg gtctgaccgg caaggaggcc gaggccatcc
      181 tgggccaggc ccacatgacc tgcaacaaga acggcatccc caacgacccg cagaagccca
      241 tggtgacgag cggcatccgc ctcggcagcc cggccttcac gacgcgcggc ttcaaggagg
      301 cggaagcggc gctcacggcc aacctgatcg ccgacgtgct ggacaagccg cacgacgagg
      361 ccaacatcgc ggcggtgcgc gccaaagtcg ctgcgctgac acgcgacttc cccgtctatc
      421 gctgaggagc cagcgaggcc gggtcctgaa cgaatccggc ctcatcgacc atgcgctgcc
      481 cattttgcgg ccacgaagac acccaggtcg tcgagacgcg cgagtccgac gaaggcgacg
      541 tcatacgccg gcgccggcgc tgcccggcct gcgacaagcg cttcacgacc tatgagcgcc
      601 ccgagctcgc gatgccggcc atcgtcaaga aggacggcag ccgcagcgag ttcgaccgca
      661 acaaaatccg cgcctccatg ctgctggcgc tgcgcaagcg gccggtgagc atcgaccaag
      721 tcgacagcgc cttggcccgt atcgaagaga agctcctgtc cagcggcgcc aacgaagtgc
      781 catcggcccg catcggcgag atggtgatgc gcgaactcaa gaagctggac aaggtcgcct
      841 acgtgcgttt tgcctcggtc taccgcagct tcgagggcgt ggacgagttc agccgcttga
      901 tcaaggacat ctgagctgaa cgactgccgg cccgatcagc gcggccggcg actgcttccc
      961 cctcctcttc ctgttctttc gccgccggct ataccttgct ggaaatcctg gtcgccgtgc
     1021 tggtgcggcg gcttcttggc caaccgcctc gtcttcacca atggagatcc gcgcgagaac
     1081 cgcacggtgg tgctggcgcc ctcgggctgc gcccgtatct gcaaggtcgg tgacgctgcc
     1141 tgcttggcag acaacagcta agctgctgac gttttgggta gcatgcacgc gatgcggcgt
     1201 gatgctgcat tgtctgctcc gcatgcccca cacattctca ccgcgcgatg aagagctgat
     1261 gcgcctggcc ctggccgagg cgcaacaggc tttgccgctc agcccgccca acccggccgt
     1321 cggctgcgtg atcgccgacg ccgaagggca gatcctgggc cagggccaca cccagctcgc
     1381 aggtggcccg catgccgaag taatggcgct gcgcgcggca cggacgcgcc ggcccgatct
     1441 cgccggctgc accgcctatg tcacgctgga gccctgcagc caccacggcc ggacgccgcc
     1501 ctgcgtgaac gcgctgatcg agcacagcat cgcgcgcgtc gtggccgccg tccaggaccc
     1561 gaatccgcta gtggccgggc gtgggcttgc gatgctgcgc gaggccggcg tgcaggtcga
     1621 actgggtctg ctcggcgagc aggcgcgcga gctgaatatc ggcttcttct cgcgcatggt
     1681 ccggcggcgc ccctggctgc ggatgaaggt cgcggcctcg ctggacggcg tcaccgccct
     1741 ggacaacggt gccagccaat ggatcacctc cgaggccgcg cgcaccgacg gccaccgctg
     1801 gcgtgcacgc gccggcgctg tgctgaccgg ggtgggcacg gtgcgcgagg acgatccgcg
     1861 gctggacgtg cgcctggtgc ccacacagcg ccagccgata cgcgtgatcg tcgattcgcg
     1921 gctggagacc ccgcgccagg cgcacatcct cgcaccgccc ggcgaggtgc tgatctactg
     1981 tgcctcggcc accaggcagg atgcagcaga attccaggcg ctcggcgccc agctcatcgc
     2041 gctgccgaat caggcaggca aggtcgaact ggctgcgatg ctgaaggacc tggcccagcg
     2101 cggcatcaac gagctgcatg tcgaggccgg ccaccgcctc aacggctcgt tgctgcgcga
     2161 aggcctggtg gacgaattcc tgatctacct ggcgcccaag cttctgggcc agggccggag
     2221 catggccagc ttcgggccgc tgcaccgcct gcaggacgca gtcgagctgg aattcctcga
     2281 tgtcgaacat gtcgggccgg accttcgcat cgtcgccagg ccgccgggac gcgcgacttt
     2341 ctaagaccag cgctcgatcc ccccaaccgg caggggcgcg aagaccgccg cctacaatcc
     2401 gggcatggct atctcccctg tccccgagct catcgccgag ctagctgccg gccgcatggt
     2461 catcctcgtc gacgaggaag atcgcgagaa cgagggcgac ctcgtgctgg cggccgacca
     2521 tgtcacgccg gaggccatca atttcatggc caagtacggc cgtgggctga tctgcctcac
     2581 gctcacgcgc gagcgctgcg agcggctgca gttgccgccc atgacccagc gcaacggcgc
     2641 ccagcatggc acggccttca ccgtctcgat cgaggccgca gagggcgtga cgaccggcat
     2701 ctcggccgcc gaccgtgcgc acaccgtcaa ggtcgcggtg gcgcgcgaat gccgagcgcg
     2761 cgacctggtg cagccgggcc acgtcttccc gctgcaggcc caggacggcg gcgtgctgat
     2821 gcgcgccggc cataccgaa
//
LOCUS       DASUCY010000124         3466 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_211513, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000124 DASUCY010000000
VERSION     DASUCY010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211513"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VFV25_02445"
     CDS             complement(<1..245)
                     /locus_tag="VFV25_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HEY1392203.1"
                     /translation="MKATLRQQFDRLAVRLQELDAALAEPDVASDIKRYRSLTREHAE
                     LDGLVGRYRRYQQREVDLQAARELLADPDMAELARDE"
     gene            complement(306..1649)
                     /gene="hemA"
                     /locus_tag="VFV25_02450"
     CDS             complement(306..1649)
                     /gene="hemA"
                     /locus_tag="VFV25_02450"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEY1392204.1"
                     /translation="MSVFALGLNHNTAPVDLRGRFAFTLEQLAPTLQGFRQRLEGHHE
                     AARAPEAAILSTCNRTELYCAAEPRLVRPALEWLAGVGGVGPDALKHHAYVLEDGEAA
                     RHAFRVASGLDSMVLGEPQILGQLKQAVHEADTAGTLGTTLHQLFQRSFAVAKEVRTS
                     TEIGAHSISMAAASVRLAAQLFEDLREIKVLFVGAGEMIELAATHFAARTPKHMAVAN
                     RTLERGEKLASRLGAEAMRLADLPARLHEFDAVVSCTASTLPIIGLGAVERALKARRH
                     RPMFMVDLAVPRDIEPEVAQLGDIYLYTVDDLSALVQSAGEKRLAAVAQAEAIIETGV
                     QSFVHWLDQRATVPLIQALQAQTDSWREHEIARARKLLAKGEDIEAVLDALSRGLTQK
                     MLHGTLAELHGADNAQRQQLADTVSRLFLRGDWSSPGLRSTAARKPGGDDTSSHS"
     gene            1782..2057
                     /locus_tag="VFV25_02455"
     CDS             1782..2057
                     /locus_tag="VFV25_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392205.1"
                     /translation="MGPLDALNHLVNFFMPALGVGLISALLAKLLWYRSLKGVGWLRL
                     AGWSTAACALVSVVGLVLSGRDGRMLTYVAMAAGCALALLWAGWGRR"
     gene            2068..2703
                     /gene="queE"
                     /locus_tag="VFV25_02460"
     CDS             2068..2703
                     /gene="queE"
                     /locus_tag="VFV25_02460"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HEY1392206.1"
                     /translation="MSYSVKEIFYTLQGEGSHAGRPAVFCRFAGCNLWSGREADRASA
                     VCKFCDTDFVGTDGTLGGKFATAAELAGRVAAQWPGADLSARFVVLTGGEPLLQVDEA
                     LVAALHAQGFEIAVETNGTVAAPAGIDWLCVSPKAGAPLVQRAGQELKVVVPQEGLDL
                     AELEKLDFEHHRVQPMDGPQRQANTEWAVQWCLDHPRWQLSLQTHKYLGIR"
     gene            2712..3086
                     /locus_tag="VFV25_02465"
     CDS             2712..3086
                     /locus_tag="VFV25_02465"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEY1392207.1"
                     /translation="MFELSQSFYFESAHTLRRKVERNEADGSRRVHGHTYTAEVSVRG
                     QIDPETGMLVDLAVLRAAIATVRAKLDHHFLDEVPELGAPTLENLCVFIHKQLSAAVP
                     GVCEVSVERKSSGDRCSYRPAA"
     gene            3083..>3466
                     /locus_tag="VFV25_02470"
     CDS             3083..>3466
                     /locus_tag="VFV25_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003071797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY1392208.1"
                     /translation="MSEASLPERSLRAVWHPCTQMKRHEREPVLAIVRGEGPWLCDDQ
                     GRRYLDAVSSWWVNLFGHQHAGIKAALVDQLDRIEHVMLAGLTHEPVVALSERLAALT
                     GLGHAFYGSEGASATEIALKMSAHYW"
BASE COUNT          518 a         1151 c         1217 g          580 t
ORIGIN      
        1 atctcgtcgc gcgccaactc ggccatgtcg gggtctgcga gcagctcgcg cgccgcttgc
       61 agatcgacct cgcgctgctg atagcggcgg tagcggccca ccaggccgtc gagctcggca
      121 tgctcgcgcg tcaagctgcg atagcgcttg atgtcgctcg ccacgtcggg ctcggccagg
      181 gcggcgtcca gctcttgcaa gcggacggcg aggcggtcga attgttggcg cagcgttgct
      241 ttcatgaagg cgggatgtga ggcgaggccg cctgcgcgcg gcaggcggca aaaacgagga
      301 gggagctagg agtgcgacga ggtatcgtcg ccgccgggct tgcgggccgc ggtgctgcgc
      361 agccccggcg acgaccaatc gccgcgcagg aagaggcgcg acaccgtgtc cgccagctgc
      421 tggcgctggg cgttgtcggc gccatgcagc tcggccagcg tgccgtgcag catcttctgc
      481 gtgaggccgc ggctcagcgc atccagcacg gcctcgatgt cctcgccctt ggcgagcagc
      541 ttgcgggcgc gcgcgatttc gtgctcgcgc cagctgtcgg tctgggcctg gagggcctgg
      601 atcaagggca ccgtggcgcg ctggtccagc cagtgcacga agctttgcac gccggtttcg
      661 atgatcgcct cggcctgggc caccgcagcc agacgcttct cgccggcgct ttgcaccagg
      721 gccgagagat cgtcgaccgt gtacagatag atgtcgccga gctgcgccac ctcgggctcg
      781 atgtcgcgcg gcacggcgag gtcgaccatg aacatcgggc gatgccggcg ggccttgagc
      841 gcccgctcga cggcgcccag gccgatgatg ggcagggtgc tggcggtgca ggacacgacg
      901 gcatcgaact cgtgcaggcg agccggcagg tccgccaggc gcatcgcctc ggcgcccagg
      961 cgcgaggcga gcttctcgcc gcgctccagc gtgcggttgg ccacggccat gtgcttgggc
     1021 gtcctggcgg cgaagtgcgt cgccgccagc tcaatcatct cgcccgcgcc gacgaacaac
     1081 accttaatct cgcgcaggtc ctcgaacagc tgcgccgcca acctcaccga ggcggccgcc
     1141 atgctgatcg aatgcgcgcc gatctcggtg gaggtacgca cttccttggc gactgcgaag
     1201 ctgcgctgga agagctggtg cagcgtggtg ccgagcgtgc cggccgtgtc ggcctcgtgc
     1261 accgcttgct tcagctggcc caggatctgg ggctcgccca gcaccatgga atcgagcccg
     1321 ctggcgacgc gaaaggcatg gcgggcggcc tcgccgtcct ccagcacata ggcgtgatgc
     1381 ttgagcgcat cggggccgac gccgccgacg ccggccagcc attccagcgc cgggcgcacc
     1441 agcctcggct cggcggcgca atacagctcg gtgcggttgc aggtcgacag aatggccgct
     1501 tccggcgcgc gcgcagcctc gtgatggcct tccaggcgct gccgaaagcc ctgcagcgtg
     1561 ggggcgagtt gctccagcgt gaaggcgaat cgcccgcgca ggtccacggg cgcggtgttg
     1621 tgattcaggc cgagggcaaa cacgctcatg ggctgattat aaaattggta gttccgtcaa
     1681 ggacttaccg gcgcaatagc cccggcgagg cgcttgatct cgatcaaggc gccacacttc
     1741 ttgcccggca gcgccttgtc ttcacttccg gaatcccagt catgggcccc ctagacgccc
     1801 tgaaccacct tgtcaatttc ttcatgcccg cgctcggcgt tgggctgatc agcgccttgc
     1861 ttgccaagct gctgtggtat cgctcgctca agggcgtggg ctggctgcgc ctggccggct
     1921 ggagtacggc tgcctgcgcc ctcgtttcgg tggtcgggct ggtgctgagc ggccgcgacg
     1981 ggcgcatgct cacctatgtg gcgatggccg ccggctgcgc tctggccttg ctgtgggcgg
     2041 ggtgggggcg gcgttgaggt cggattcatg agctacagcg tcaaggaaat cttctacacc
     2101 ttgcaaggtg agggcagcca tgcgggacgc ccggccgtgt tctgccgctt cgccggctgc
     2161 aatctctgga gcggccgcga ggccgaccgc gccagcgccg tctgcaagtt ctgcgacacc
     2221 gatttcgtcg gcaccgacgg cacgctgggc ggcaagttcg ccacggcggc cgagctggcc
     2281 gggcgggtgg cggcgcaatg gcctggtgcc gacctcagcg ctcgcttcgt cgtgctgact
     2341 ggcggcgagc ccttgcttca ggtcgacgag gccctcgtcg ccgcgctgca tgcacaaggc
     2401 ttcgagatcg ccgtcgagac caatggcacc gtggcggcac cggcgggcat cgattggctc
     2461 tgcgtcagcc ccaaggcagg tgcgccgctg gtgcagcgcg cggggcagga gctcaaggtg
     2521 gtcgtgccgc aagagggtct ggatctcgcc gagctggaga agctcgattt cgagcaccat
     2581 cgcgtgcagc ccatggacgg gccgcagcgc caggccaata ccgagtgggc ggtgcaatgg
     2641 tgcctggacc acccgcgctg gcagctcagc ttgcagacgc acaaatatct cggcatccgt
     2701 tgagccccct catgtttgaa ctgagccaat ccttctattt cgagtccgcc catacgctgc
     2761 ggcgcaaggt cgagcgcaac gaggccgacg gcagccgccg cgtgcatggc cacacctaca
     2821 ccgccgaagt gagcgtgcgg ggccagatcg atcccgagac cggcatgttg gtggacctcg
     2881 ccgtgctgcg cgcggcgatc gccaccgtgc gggccaagct ggatcatcat ttcctcgacg
     2941 aggtgcccga gttgggcgcg ccgaccctgg agaacctttg cgtcttcatc cacaagcagc
     3001 tgagcgccgc cgtgccgggc gtgtgcgagg tgagcgtgga gcgcaagagc agcggcgacc
     3061 gctgcagcta tcgcccggcc gcatgagcga agcttccctg ccggaacgca gccttcgcgc
     3121 cgtctggcat ccctgcacgc agatgaagcg ccacgagcgc gagcctgtgc tcgccatcgt
     3181 gcgcggtgag gggccctggc tctgtgacga ccagggcagg cgctatctcg atgccgtgag
     3241 ttcctggtgg gtcaatctct tcggtcatca gcatgccggc atcaaggcgg cgctggtcga
     3301 ccagctcgat cgcatcgagc acgtgatgct ggccggtctc acgcatgaac cggtggtcgc
     3361 gctgtccgag cgcctcgcgg cgctgaccgg gcttggccac gccttctacg gcagcgaggg
     3421 agcctcggct accgagatcg cgctgaagat gagcgcccac tactgg
//
LOCUS       DASUCY010000125         3589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_147283, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000125 DASUCY010000000
VERSION     DASUCY010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147283"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="VFV25_02475"
     CDS             complement(<1..545)
                     /locus_tag="VFV25_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY1392209.1"
                     /translation="MPVGTTLSQRKFDVVIVGAGGSGMRASLQLARAGLNVAVLSKVF
                     PTRSHTVAAQGGIGASLGNMSEDNWHYHFYDTIKGSDWLGDQDAIEFMCREAPKVVYE
                     LEHFGMPFDRNPDGTIYQRPFGGHTANYGEKPVQRACAAADRTGHALLHTLYQQNVKA
                     RTQFFVEWMALDLIRDADGDVV"
     gene            complement(545..910)
                     /gene="sdhD"
                     /locus_tag="VFV25_02480"
     CDS             complement(545..910)
                     /gene="sdhD"
                     /locus_tag="VFV25_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEY1392210.1"
                     /translation="MTQNYGSKRLVVGAHYGLTDWLAQRVTALLMALFTIVLLAQVLF
                     TRGPIGYDKWAHIFSHQWMKVLSFVVIASLLYHAWVGMRDIWMDYIQPVGVKLALQVF
                     TIVWLLGCAGWAVQVLWRL"
     gene            complement(944..1384)
                     /gene="sdhC"
                     /locus_tag="VFV25_02485"
     CDS             complement(944..1384)
                     /gene="sdhC"
                     /locus_tag="VFV25_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEY1392211.1"
                     /translation="MAEILSTQSAKQRPVFRNIHITQIVAYRLPPAGIVSILHRVSGL
                     LMFLLLPFIIWMFDNSLTDEISYDRFTSVFAGGGLFPGWFVKLVVLALIWAYLHHFIA
                     GLRHLWMDATHSVSKQQGHSSAVFTLAASVALTLVLGAKLFGLY"
     gene            complement(1497..2267)
                     /locus_tag="VFV25_02490"
     CDS             complement(1497..2267)
                     /locus_tag="VFV25_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY1392212.1"
                     /translation="MSTPSSSLPGAPAADAGGPAFSPLYQQIKALLMRSLQAGEWRPG
                     EAIPSEVELAVRFKVSQGTVRKAIDELATENLLVRRQGKGTFVATHSEEQIQYRFLRL
                     APDDGGPIGPVERKLLDCRRLRAPAEVARALDLKSGDTAFQLRRLLIFRGKPVVLEEI
                     WLPGQPFKGLTAERLSDYRGPMYAFFEAEFGVRMIRAEEKLRAVAAEPSVAELLQQPV
                     GTPLLSIERLSLTYGDKPVELRRGLYHTAAHFYRNELS"
     gene            2471..3457
                     /locus_tag="VFV25_02495"
     CDS             2471..3457
                     /locus_tag="VFV25_02495"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEY1392213.1"
                     /translation="MSKKPVRVAVTGAAGQIGYALLFRIASGEMLGKDQPVILQLLEI
                     PDEKAQKALKGVMMELEDCAFPLLAGMEAHSAPDTAFKDTDYALLVGARPRGPGMERK
                     DLLSANAQIFTAQGKALNAVASRNVRVLVVGNPANTNAYIAMKSAPDLPRKNFTAMLR
                     LDHNRALSQVAAKTGKAVADIEKLCVWGNHSPTMYADYRYATIGGQSVKAMINDQEWN
                     ANVFLPTVGKRGAAIIEARGLSSAASAANAAIDHMHDWALGTNGKWVTMGIPSDGQYG
                     IPKDVMFGFPVTTANGEYKVVEGLEIDAFSQERINLTLKELLEEQDGVKHLL"
BASE COUNT          680 a         1209 c         1141 g          559 t
ORIGIN      
        1 accacgtcgc catcggcgtc gcggatcagg tcgagcgcca tccattcgac gaagaactgg
       61 gtgcgcgcct tgacgttctg ctggtacagc gtgtgcagca gcgcgtggcc ggtgcggtcg
      121 gccgccgcgc aggcgcgctg cacgggcttc tcgccgtagt tcgccgtgtg gccgccgaag
      181 gggcgctggt agatcgtgcc gtccgggttg cggtcgaagg gcatgccgaa atgctcgagc
      241 tcgtagacga ccttgggcgc ttcgcggcac atgaactcga tcgcgtcctg gtcgccgagc
      301 cagtcggaac ccttgatcgt gtcgtagaag tggtagtgcc agttgtcctc ggacatgttg
      361 cccagcgagg ccccgatgcc gccttgcgcc gccacggtgt gggagcgggt cgggaagacc
      421 ttggacagca cggccacgtt gaggccggcg cgcgccagct gcagcgaggc gcgcatgccg
      481 gagccgccgg ccccgacgat cacgacgtcg aacttgcgtt gggaaagggt tgttccaacc
      541 ggcatcacag tctccacagc acctgaacgg cccagccggc gcagccgagc aaccaaacaa
      601 tcgtgaacac ctgcagcgca agcttcacac ccaccggctg gatgtagtcc atccagatgt
      661 cacgcatgcc gacccaggcg tggtacagca gcgaagcgat cacgacgaag ctcagcactt
      721 tcatccactg atgcgaaaag atatgggccc acttgtcgta gccgatcggg cccctggtga
      781 acagcacttg cgccagcaac acgatcgtga agagggccat cagcagcgct gtcacgcgct
      841 gggcgagcca gtcggtgagg ccgtagtgcg caccgaccac gaggcgcttg gagccgtagt
      901 tctgcgtcat tgcgtttcct tgttttcttg attcccgcgc ggctcagtac aggccgaaca
      961 gcttggcgcc cagcaccagc gtcagcgcga cgctggccgc cagcgtgaac accgccgagg
     1021 aatgcccctg ctgcttgctc acgctgtgcg tcgcatccat ccacagatgg cgcagcccgg
     1081 cgatgaagtg atgcagatag gcccagatca gcgccagcac caccagcttg acgaaccagc
     1141 ccggaaacaa gccgccgccc gcgaagacgc tagtgaagcg gtcgtaagag atctcgtcgg
     1201 tcaggctgtt gtcgaacatc cagatgatga agggcagcag cagaaacatc agcaagccgc
     1261 tgacgcgatg caggatcgac acgatgcccg ccggcggcag gcggtaggca acgatctgcg
     1321 tgatgtggat gttgcgaaac accggtcgtt gtttcgcgct ctgggtgctg agtatttctg
     1381 ccattgccgc gttgctccag gcttgcgtaa cgtgagtgaa actcgaagat tctaggacaa
     1441 ggatttggcg tcgccgggcc tgccgaacgc gcatttcacg gcttgcagcg cgcgcatcag
     1501 ctcaactcgt tgcgatagaa atgcgcggcc gtgtgataaa gcccgcgacg cagctccaca
     1561 ggcttgtcgc cgtaggtcag cgacaggcgc tcgatgctga gcagcggcgt gcccacgggc
     1621 tgctgcagca gctccgccac cgaaggctcc gcggccacgg cgcgcagctt ctcttcggcg
     1681 cggatcatgc gcacgccgaa ctcggcctcg aagaaggcat acatcggtcc gcggtagtcg
     1741 ctcaggcgtt ccgcggtcag ccccttgaag ggctgcccgg gcagccagat ctcttccagc
     1801 accaccggct tgccgcggaa aatcagcagc cggcgcagct gaaaagccgt gtcgcctgac
     1861 ttgagatcga gggcacgcgc cacttccgcc ggggcgcgca ggcgccggca atcgaggagc
     1921 ttgcgctcca cggggccgat cgggccgccg tcatcgggcg ccagccgcag aaagcggtac
     1981 tggatctgct cttcggagtg cgtcgcgacg aaggtgccct tgccctggcg gcgcaccagc
     2041 aggttctcgg tggcgagctc gtcgatggcc ttgcgcaccg tgccctggct gaccttgaag
     2101 cgcaccgcca gctcgacttc gctggggatc gcttcgccgg ggcgccactc gccggcctgc
     2161 aggctgcgca tcagcagggc cttgatctgc tgatagagcg ggctgaacgc cgggccgccc
     2221 gcatccgccg cgggggcacc tggaagcgag gaggaaggcg tggacatccg gtcattgcac
     2281 cacaggctcg ccccggcgtc caccagaccc atgattaatg tcttatataa gacataggat
     2341 gattgacgac cccgggatca acacctagac tggcctaaac tccccaacag acaggcgcaa
     2401 gccgcgaaac gtccgcagga tcaggcgcct ctccccccaa ttcccctctt tctccacgga
     2461 gtcacacacc atgagcaaga aacccgtacg cgtcgccgtc accggcgccg ccggccagat
     2521 cggctatgcc ctgctgttcc gcatcgcctc cggcgagatg ctgggcaagg accagcccgt
     2581 gatcctgcag ctgctcgaga tccccgacga gaaggcccag aaggcgctca agggcgtgat
     2641 gatggagctc gaggactgcg ccttcccgct gctggccggc atggaggcgc acagcgcccc
     2701 cgacaccgcc ttcaaggaca ccgactacgc cctgctcgtc ggcgcccgcc cgcgcggccc
     2761 cggcatggag cgcaaggacc tgctgtcggc caatgcgcag atcttcacgg cccagggcaa
     2821 ggcgctcaac gccgtggcca gccgcaacgt cagggtgctg gtggtgggca accccgccaa
     2881 taccaatgcc tacatcgcga tgaagtcggc gcccgacctg ccgcgcaaga acttcaccgc
     2941 catgctgcgc ctggaccaca accgcgcgct gagccaggtc gccgccaaga ccggcaaggc
     3001 cgtggccgac atcgagaagc tgtgcgtgtg gggcaaccat tcgccgacca tgtacgccga
     3061 ctaccgctac gccaccatcg gcggccagag cgtcaaggcc atgatcaacg accaggaatg
     3121 gaacgccaac gtcttcctgc ccacggtggg caagcgcggc gccgccatca tcgaggcgcg
     3181 cggcctctcg tccgcggctt ccgcggccaa cgcggccatc gaccacatgc acgactgggc
     3241 cctgggcacc aacggcaagt gggtcacgat gggcatcccg tccgacggcc aatacggcat
     3301 ccccaaggac gtgatgttcg gcttcccggt caccacggcc aacggcgaat acaaggtggt
     3361 cgagggcctg gagatcgacg ccttctcgca ggaacgcatc aacctcacgc tgaaggaact
     3421 gctcgaggag caggacggcg tcaagcatct gctgtaagtg ctccacaagg ccggctggat
     3481 gacccgccgg ccttttccgc tcgcggaaac gctgcgccga gctgcgagta ccggcccaag
     3541 ctggacgagg gaatgagcga ctggaacccg gccctctacc gccgctacg
//
LOCUS       DASUCY010000126         3676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_18828, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000126 DASUCY010000000
VERSION     DASUCY010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18828"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="VFV25_02500"
     CDS             complement(<1..875)
                     /locus_tag="VFV25_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY1392214.1"
                     /translation="MKFLRHLALVGLFCAAALPALADINVGITLSATGPAASLGIPEK
                     NTIALMPTSIGGQKVNYIVLDDASDTTTAVTNARKLITENKVDVLLGSTTSPNSLAMI
                     DVAAENQTPMISMAASARIVEPVDAKRRWVFKTPQNDIMMSLAIVGHMADLGIKKVGY
                     IGFADAYGEGWYQEFSKAAALKKIEIVANERYNRTDTSVTGQVLKLLAAKPDAVLVGA
                     SGTPAVLPQRTLKERGYAGKIYQTHGVANNDFLRVGGKDVEGTFLPAGPVLVATQLPA
                     DHPVRKSALDYVGKY"
     gene            complement(924..1562)
                     /locus_tag="VFV25_02505"
     CDS             complement(924..1562)
                     /locus_tag="VFV25_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY1392215.1"
                     /translation="MARGRAANYDEQRELILANAAQLFAQRGYSATSMNEVAEACGLS
                     KPTLYHYFRDKYELLVSIAENHVSLLQAVVDEVMQRPLPSGAPPETRLRELILRFVEE
                     YGGAQHAHRVLTEDVKFLHTADRERILGKEREVVGGFARAVAELRPEAKGDGLNKPLT
                     MLLFGMINWMFTWLRPDGELDHKAMAPIVADLFFGGLGAVHSPQREPESKAA"
     gene            complement(1591..2697)
                     /gene="paaE"
                     /locus_tag="VFV25_02510"
     CDS             complement(1591..2697)
                     /gene="paaE"
                     /locus_tag="VFV25_02510"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HEY1392216.1"
                     /translation="MKTHFHPLRVRAIQPDTEEAMIVSFDVPTELREVFGFMQGQYLT
                     LRQEIDGQDLRRSYSICAGLDDGELRVGVRKVRGGMFSNWINTHLKPGDVIQVMPPQG
                     RFYVPIAPQAHRHHVGIAGGSGITPILSIMKTVLAREPHSSFTLIYGNRSQKSTMFKE
                     EIEDLKNRYLTRLVLHHVFSDEHTDAPLNSGVMNREKIAAFLEVLVPAAGIDHVYVCG
                     PYQMNDEAEAALLAAGVPEERIHIERFGIAPPVAGEAAGAAQAAGAVMHETKAGDAEQ
                     ARVLIIRDGLSREILFRKDDPSILDAASTAGLEVPYSCTSGVCGTCRAKLMEGQVRMD
                     RNFALEKHEVAAGFVLTCQAHPLTERVVLSFDER"
     gene            complement(2719..3261)
                     /gene="paaD"
                     /locus_tag="VFV25_02515"
     CDS             complement(2719..3261)
                     /gene="paaD"
                     /locus_tag="VFV25_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HEY1392217.1"
                     /translation="MVSAVLQARTEARAVRVASAWAVLAEVLDPEVPALSVCDLGIVR
                     DVLELGDGTLEIVLTPTYSGCPATEAIERNVLDAIEAAGLGPARAKLQRAPAWTTDWI
                     SEEGRRKLHEYGIAPPAHLAAAQAAATPIRFMRREAALACPRCGSKDTERLSAFGSTA
                     CKALYRCRACREPFEHFKPI"
     gene            complement(3255..>3676)
                     /gene="paaC"
                     /locus_tag="VFV25_02520"
     CDS             complement(3255..>3676)
                     /gene="paaC"
                     /locus_tag="VFV25_02520"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HEY1392218.1"
                     /translation="KLHESSDAELAAIAGKAIKEARYHQQHAGEWLLRLGDGTDESRR
                     RTEAALQELWRFAGEFFESDAVDEQAAASKLGPCWADLREAWEAEMTALLGEADLAVP
                     QASAFRSTGRQGVHSEHMGYILTEMQYLQRAFPGGVW"
BASE COUNT          554 a         1233 c         1240 g          649 t
ORIGIN      
        1 tcgtacttgc ccacgtagtc gagcgcggac ttgcgcaccg gatgatcggc cggcagctgg
       61 gtcgccacca gcaccgggcc ggccggcagg aaggtgcctt cgacatcctt gccgcccacg
      121 cgcaggaagt cgttgttggc cacgccatgg gtctggtaga tcttgcccgc gtagccgcgc
      181 tccttgagcg tgcgctgcgg cagcacggcc ggcgtgccgg aggcgccgac cagcaccgcg
      241 tcgggcttgg cggccagcag cttgagcacc tggccggtca ccgaggtgtc ggtgcggttg
      301 tagcgttcgt tggcgacgat ctctatcttc ttcagcgccg cggccttgct gaactcctgg
      361 taccagcctt cgccgtaggc gtcggcgaag ccgatgtagc ccaccttctt gatgcccaga
      421 tcggccatgt ggcccacaat ggccagcgac atcatgatgt cgttctgcgg tgtcttgaag
      481 acccagcgcc gcttggcatc gaccggctcg acgatgcgtg cgctggcggc catggagatc
      541 atcggcgtct ggttctcggc cgccacgtcg atcatcgcca gcgaattggg cgaggtcgtg
      601 gagccgagca ggacgtcgac cttgttctcg gtgatgagct tgcgcgcatt ggtgacggcg
      661 gtggtcgtgt ccgaggcatc atcgagcacg atgtagttga ccttctgccc gccgatgctc
      721 gtgggcatca gcgcgatcgt gttcttctcg gggatgccca gcgatgccgc ggggccggtg
      781 gccgacagcg tgatgccgac attgatgtcg gccagggcgg gcaaggccgc agcgcagaac
      841 agccccacca gggccaggtg tcgcaggaac ttcatcgctt ggtctccggt tgttgatcgt
      901 gttggtcgcg gaagtatcgc ggcttacgcc gccttggact cgggctcgcg ctgcggcgag
      961 tgcaccgcgc ccaggccacc gaagaagaga tcggccacga tgggcgccat cgccttgtgg
     1021 tccagctcgc catcgggcct gagccaggtg aacatccagt tgatcatgcc gaagagcagc
     1081 atggtcagcg gcttgttgag cccatcgccc ttggcttcgg ggcgcagctc ggccacggcg
     1141 cgcgcaaagc cgccgaccac ctcgcgctcc ttgccgagga tgcgttcgcg atcggcggta
     1201 tggaggaatt tcacgtcctc ggtcagcacg cggtgggcat gctgggcgcc gccgtattcc
     1261 tcgacgaagc gcagtatcag ctcgcgcaag cgcgtctcgg ggggcgcccc ggaggggagc
     1321 ggccgctgca tcacctcgtc caccacggct tgcagcagcg agacatggtt ttccgcgatg
     1381 ctgaccagca gctcgtactt gtcgcggaag tagtgataaa gcgtcggctt cgacaagccg
     1441 caggcttcgg ccacctcgtt catcgaggtc gccgaatagc cgcgctgcgc gaacagctgc
     1501 gccgcattgg ccaggatcag ctcgcgctgc tcgtcgtagt tggcggctct gccacgggcc
     1561 atggatgcct ttcgttcctc gctcgctctc tcagcgctcg tcgaaggaca agaccacacg
     1621 ctcggtcagc gggtgggcct ggcaggtcag cacgaagccg gccgcgacct cgtgcttctc
     1681 gagcgcgaaa ttgcggtcca tgcgcacctg gccttccatc agcttggcgc ggcaggtgcc
     1741 gcatacgccg gaggtgcagg agtaaggcac ctccagcccg gccgtcgacg ctgcgtcgag
     1801 gatgctcgga tcgtccttgc ggaacaggat ctcgcggctc aggccgtcgc ggatgatgag
     1861 cacgcgtgcc tgctcggcat cgcccgcctt ggtctcgtgc atcaccgccc cagccgcctg
     1921 ggccgcgcca gcggcctcac ctgcaacagg cggcgctatg ccgaagcgct cgatgtggat
     1981 gcgctcctcg ggcacgccgg cggcgagcag cgccgcctcg gcctcatcgt tcatctgata
     2041 ggggccgcag acatagacat ggtcgatgcc ggccgccggc accagcacct ccaggaaggc
     2101 ggcgatcttc tcgcggttca tcacgccgct gttcagcggc gcatcggtgt gctcgtcgga
     2161 aaagacatgg tgcagcacca gccgcgtcag atagcggttc ttcagatcct cgatctcctc
     2221 cttgaacatc gtggacttct ggctgcggtt gccgtagatc agcgtgaagc tcgaatgcgg
     2281 ctcgcgcgcc agcaccgtct tcatgatcga gaggatgggc gtgatgccgc tgccgccggc
     2341 gatgcccaca tgatggcggt gcgcctgcgg ggctatgggc acgtagaagc ggccctgagg
     2401 cggcatcacc tggatcacgt cgccaggctt cagatgggtg ttgatccagt tggagaacat
     2461 gccacccctg accttgcgca cgcccacgcg cagttcgccg tcgtccaggc cggcgcagat
     2521 cgaataggag cggcgcaggt cctgtccgtc gatctcttgg cgcagcgtga ggtattggcc
     2581 ctgcatgaag ccgaagactt cgcgcagctc agtcggcacg tcgaaggaga cgatcatcgc
     2641 ctcctcggtg tccggctgga tggcgcgcac gcgcagggga tggaagtggg tcttcatgta
     2701 gggcctctgt gcagccgctc agatgggctt gaaatgctcg aagggctcgc ggcaggcgcg
     2761 gcagcgatac aacgccttgc aggccgtgga gccgaaagcc gacaggcgct cggtgtcctt
     2821 gctgccgcag cgcgggcagg ccagcgcagc ctcgcggcgc atgaagcgga tcggcgttgc
     2881 ggcggcctgc gccgccgcca gatgagcagg cggcgcgatg ccgtattcgt gcagcttgcg
     2941 ccggccttcc tcgctgatcc agtcggtggt ccaggccggc gcgcgctgga gcttcgcgcg
     3001 agccgggccc agcccggccg cttcgatggc gtccagcaca ttgcgctcga tcgcctcggt
     3061 ggccgggcag ccggaatagg tcggcgtcag cacgatctcc agcgttccgt cgccgagctc
     3121 cagcacatcg cgcacgatgc ccaggtcgca caccgaaagc gccggcacct cggggtccag
     3181 cacctcggcc agcacggccc aggcgcttgc cacgcgcacc gcgcgtgcct ctgtccgggc
     3241 ttgcagcacg gcactcacca cacgccccct ggaaaggcgc gctgcaggta ttgcatctcg
     3301 gtcaggatgt agcccatgtg ctcgctgtgc acgccctgcc tgccggtgct gcggaaggcg
     3361 ctggcctggg gcacggcaag atcggcctcg ccgagcagcg ccgtcatctc ggcctcccag
     3421 gcttcccgca gatcggccca gcacgggccc agcttgctcg cggcagcctg ctcgtcaacc
     3481 gcgtcggact cgaagaactc gccggcgaag cgccacagct cctgcaaggc cgcttcggtg
     3541 cgccggcgcg attcgtcggt gccgtcgccc aggcgcagca gccactcgcc tgcgtgctgc
     3601 tggtggtagc gcgcctcctt gatcgccttg ccggcgatgg ccgccagctc agcgtcgctg
     3661 gactcgtgca gcttct
//
LOCUS       DASUCY010000127         5301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_388271, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000127 DASUCY010000000
VERSION     DASUCY010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 5301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5301
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388271"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1219)
                     /locus_tag="VFV25_02525"
     CDS             complement(<1..1219)
                     /locus_tag="VFV25_02525"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEY1392219.1"
                     /translation="MTLTLRAVSLNDQPLTQPITAQFGAEGGTIGRADQNTLALPDPE
                     RHISRHQAEISVHGDSYAIKNIGSANPIIVHGQPLAQGVSAALTHHDQVRIGGYLLEV
                     IDDGLLGTDTTTVTRPRTAPAEPKTPMRTAAALAAAQLAAPPQVAPLSSSNPFADLLG
                     PAPAAAPMQAGAVPAAAKTDDPFADLPPPAGLSGASAAWAAAAPAAPRLPDDFDPFAA
                     SAPAAASPLSAPAPGGAGAFDDLIPSAAAPSIDEAFGLQQSGSSQDPLAAFMAGAPAQ
                     AAGSEPRAASGLSTDPLALFGGTKAEAPGMPTTPDDASELRGAFKPPGLRDTPDPLLP
                     SGLDWLAQSPKRPAATVPPMPPTAARSNEAQRPAHAVTPPEAPEAPEAAAVHAAANKA
                     AASAEAGAWAGDTQ"
     gene            complement(1389..2657)
                     /locus_tag="VFV25_02530"
     CDS             complement(1389..2657)
                     /locus_tag="VFV25_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02270 family protein"
                     /protein_id="HEY1392220.1"
                     /translation="MTQQLVRPVIPVVVQQHAEESAMLRHIRSVLVRAPHVKLHHLRR
                     LDDRIAAHLDGLSVAGAYGTECATAALARPGAGEVFAVAVRAIEDRDDKALDRLIALA
                     GALPDGKRGLLSAFGWVSAGQLQGIARTLLVSDDPLRREIGLAACRLHRTDPGAVLAS
                     ALRDSRPPLMIAALRAAGELGRIDLLEHVMAAMTEEAEDVVFWAARSACLLGERPASS
                     KVLSALAQREGERAEPALLLLLQAQAFEPAREQLRAASKAVDKPALKRRLIRGFGLLG
                     DAQFLPWLVDLMADDHLARLAGESFSMITGADLAALDLERKPPEGFEARPNDDPEDEN
                     VAMDEDDSLPWPDRDRIQRWWQARSAQMRADRRLFLGEPPTPEHCLRVLREGYQRQRI
                     AAARELCLLKPGTPLFAVCAPVWRQQRALG"
     gene            2929..3867
                     /locus_tag="VFV25_02535"
     CDS             2929..3867
                     /locus_tag="VFV25_02535"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEY1392221.1"
                     /translation="MTKKPVALLCAAVGLLAACATHPPAAPPAPASQTPGGTMPAPPE
                     PAPPQPSGPVSAAAQKQAQQIVASAIGLLEAGNEEQAKTELRRALLVNPNNDLAPNLL
                     AQIDADPLATLGRESFAYVVKPNDSLSGIAERFLHDKYKFYILARYNDIKVPRQLSSG
                     QTIRVPGKAPPPETHRPEPAPRPEAKKEAVAVPVTPPPPAAPPPPPEPSPGEKAMSSA
                     QAAERAGNLEKAYGEYHRAAGLDQAGAAEKAAQLSKQLANRYAQQARSALAKQDLDGA
                     ISAWNRVLEYDPGNDTARLERQRAITLKEKLGNLKD"
     gene            complement(3922..4752)
                     /locus_tag="VFV25_02540"
     CDS             complement(3922..4752)
                     /locus_tag="VFV25_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392222.1"
                     /translation="MSSWHPCCIAGAAIAAMLAGCAGAPPTGPAATESTPAAQISAAG
                     YERSQKERALSMARQGRLADAALSWEILTVLRPELREYRERLAETRSQIDAQVAERMQ
                     RAAQAQKRGEMDGATQLYLSVLALQPDEPRAAEALRALERDRNKRNYLGKLSAVTLTR
                     RAVADGEMAPGSAGSSNDLEHAALLAGQGELDAAIQMLERTVAGDRRNSRARQMLADF
                     YAQKAENVAPRDKQGAVSLLQKSLRMDGGNVRAQALLRQLKPEAGGAVKSEAASPGAR
                     "
     gene            complement(4749..>5301)
                     /locus_tag="VFV25_02545"
     CDS             complement(4749..>5301)
                     /locus_tag="VFV25_02545"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY1392223.1"
                     /translation="EGRQPLPRRSVVITIDDGYESVYRYAFPLLRKYGFPATLFVYTD
                     FIGAREGLSWAQLQELAASGLIDIEAHSKTHRNLIERAAGEDDERYRQDIEMEAKAPR
                     ELLERRLGVKIRNYAYPYGDANETVLAALARQQYQLAVTVNPGGNAFFAQPLMLRRTM
                     IFGDQDLEAFKAKLQISRGINAP"
BASE COUNT          845 a         1863 c         1795 g          798 t
ORIGIN      
        1 cctgcgtatc gcccgcccag gcgccggcct cggcgcttgc cgcggccttg ttcgccgcgg
       61 cgtgcacagc agccgcctcc ggcgcctccg gcgcctccgg cggcgtcacg gcgtgcgcgg
      121 gccgctgcgc ttcgttgctt cgcgcggcgg tcggcggcat aggcggcacc gtcgcagccg
      181 gccgcttggg cgactgcgca agccaatcca gccccgaggg cagcagaggg tccggcgtgt
      241 cgcgcaggcc cggcggcttg aatgcgcctc ttagctccga tgcgtcgtcc ggtgtcgtgg
      301 gcatgcccgg agcttcggcc ttcgttccac cgaaaagcgc gagcggatcg gtcgacaagc
      361 ccgacgctgc tctgggttca gagcctgccg cctgcgcggg tgcccccgcc atgaaggctg
      421 ccaagggatc ctgcgaagaa cccgattgct gcaggccgaa ggcttcgtcg atcgacggcg
      481 ctgcggcgct gggtatgagg tcgtcgaagg caccggctcc gcccggtgcg ggtgccgaca
      541 ggggcgaagc cgcggcgggc gccgaagcag cgaagggatc gaaatcgtcc ggcaacctgg
      601 gcgccgccgg ggccgccgcg gcccaggctg cggaggcacc ggaaaggccg gccggaggag
      661 gaagatcggc gaagggatcg tccgtcttcg ccgcggcagg caccgccccg gcctgcatcg
      721 gcgcagcggc aggcgccggg ccgagcaggt cggcgaaggg attgctgctg gacagcggcg
      781 ctacctgagg tggcgcggca agctgcgccg ccgccagcgc tgcagcggta cgcatcggcg
      841 tcttcggctc ggcaggcgcc gtacgcggcc gcgtgacggt cgtggtgtcc gtgccgagca
      901 gcccgtcgtc gatcacctcc agcaagtagc cgccgatgcg cacctggtcg tgatgcgtca
      961 aggccgccga cacgccctgc gccagcggct ggccgtgcac gatgatggga ttggcgctgc
     1021 cgatgttctt gatcgcatag gagtcgccgt gcacgctgat ctcggcctgg tggcgcgaga
     1081 tgtggcgctc cggatcgggc agggccagcg tgttctggtc ggcccgcccg atggtgccac
     1141 cttcggcgcc gaactgcgcc gtgatcggct gggtcagcgg ttgatcattg agcgacaccg
     1201 cgcgcagggt cagagtcatg gtgcttcacc acccatcgag ctatgggaga cacgcgctcc
     1261 gaccggcccg gcgcttcact caacagagcg cagcggccct ccgctgaaac cgacccgcac
     1321 gcatgaacca tggtggccgc cgcggtggcc cgcttcaatg aacgaaagtc atgctctgcg
     1381 ccggcggatc accccaacgc ccgctgctgc cgccataccg gcgcgcacac tgcgaacaaa
     1441 ggcgtccccg gcttcagcaa gcacagttct cgcgcagcgg cgatgcgctg gcgctgatag
     1501 ccttcgcgca gcacgcgcag gcagtgctcg ggcgtcggcg gctcgccgag gaagaggcgc
     1561 ctgtcggccc gcatctgcgc tgagcgagcc tgccaccagc gctgtatgcg atcgcggtcc
     1621 ggccagggca ggctgtcgtc ttcgtccatc gcgacgttct cgtcctcggg atcgtcgttg
     1681 ggcctggcct cgaagccctc gggcggcttg cgttcgagat cgagggccgc gaggtcggca
     1741 ccggtgatca tggagaacga ctccccggcc agccgcgcca ggtgatcgtc ggccatcaga
     1801 tcgaccagcc atggcaggaa ttgcgcgtcg cccagcaagc cgaagccgcg tatcaatcgc
     1861 cgcttgagcg caggcttgtc caccgccttg gaagctgcac gcaactgctc gcgcgccggc
     1921 tcgaacgctt gcgcctgcag cagaagcagc agcgccggct cggcccgctc gccctcgcgc
     1981 tgcgccaagg cgctcagcac cttcgaactc gcagggcgct cgccgagcag gcaggctgac
     2041 cgggcggccc agaacaccac gtcctcggct tcctcggtca tcgcggccat gacatgctcg
     2101 aggagatcga tgcggccgag ttcgcctgca gcgcgcaggg cggcgatcat cagaggcggc
     2161 cgcgaatcgc gcagagccga agccaacacc gcaccgggat ctgtgcgatg cagtcggcag
     2221 gcagcaagac cgatctcgcg gcgcagcgga tcgtcagaca ccagcagggt gcgcgctatg
     2281 ccctgtagct gcccggccga gacccagccg aaggccgaca gcagcccgcg cttgccatcg
     2341 ggcaatgcgc cggccaaggc gataaggcga tcgagcgcct tgtcgtcccg gtcctcgatc
     2401 gctcgcaccg cgacggcaaa gacctcgccg gcgcctggcc tcgccagcgc agccgtggcg
     2461 cattcggtgc cataggcccc ggccacgctc aagccgtcga gatgggccgc gatcctgtcg
     2521 tcgaggcggc gcaagtggtg cagcttcacg tgcggcgcgc gcaccagcac cgaacggatg
     2581 tggcgcagca tcgcactctc ttcggcatgc tgctgcacga ccacaggaat gaccgggcgc
     2641 acgagctgtt gcgtcatgtt ttcggcgtcg gcatggatgc acgggcaggc atcgcacgac
     2701 gaagcatgct ggatgcaggc tgcgagtgcg gcaatgaacg aaagtcattc gctgcatggt
     2761 cgaccgcggg cggccgacat gcgcccttgt gcgcggcctc gggcgcgatc aataatcggt
     2821 acggctcaac aatccagctt ggtgcgccca tgccctcggg gcgatcacgg gatcgcgaga
     2881 tcgcgcattc gtcgaggggc cgagcgctct gggaagaact tctcgcccat gaccaagaag
     2941 cctgttgcct tgttgtgcgc ggccgtcggc ctgttggcgg cctgcgcgac ccacccgccg
     3001 gcggcccctc cggcacccgc atcgcagact cccggcggca ccatgccagc cccgccggaa
     3061 cctgcgccgc cacaaccctc gggcccggtg tctgccgcgg cgcagaagca ggcccagcag
     3121 atcgtcgcct cggccatcgg gctgctcgaa gcgggcaacg aagagcaagc caagaccgag
     3181 ctgcggcgcg ccctgctcgt caacccgaac aacgatctgg cgcccaacct gctcgcccag
     3241 atcgatgccg acccgcttgc aactctgggt cgcgaatcct tcgcctacgt cgtgaagccc
     3301 aacgacagcc tgtcgggcat cgccgagcgc ttcctgcacg acaaatacaa gttctacatc
     3361 ctcgcccgct acaacgacat caaggtgccc agacagctgt cgagcggcca gacgatacgc
     3421 gtgccgggca aggcgccgcc gcccgagacc caccgcccgg agcctgcgcc acggcccgag
     3481 gccaagaaag aagcagttgc cgttcccgtc actccaccgc cacctgcagc cccgcccccc
     3541 ccgccggagc caagtccggg cgagaaggca atgagcagtg cacaggccgc ggagcgtgca
     3601 ggcaatttgg aaaaagccta tggggaatac caccgcgcgg ccggcctgga tcaggccggg
     3661 gctgcagaaa aggccgcgca gctgagcaag cagttggcca accgctacgc ccagcaagca
     3721 cgcagcgccc tcgccaagca ggacctcgat ggcgccatca gcgcctggaa ccgggtgctc
     3781 gagtacgacc ccggcaatga caccgcacgc ctggagcgac agagggcgat cactctgaag
     3841 gaaaagctgg gcaatctcaa ggattgaccg ggtcgcctgg cccttgccgc cgcggcgcag
     3901 gggcggcacg acggatcctg gctagcgcgc tccagggctc gcagcttccg acttcaccgc
     3961 gccgcccgcc tcgggcttga gctgcctgag cagagcctgc gcgcgcacat tgcccccatc
     4021 catgcgcagg ctcttttgca gcaggctcac ggcgccttgc ttgtcgcgtg gtgcgacgtt
     4081 ctcggccttc tgggcgtaga agtcggccag catctgcctg gcgcgactgt tgcgccgatc
     4141 gccagccacc gtgcgttcga gcatctgtat ggccgcatcg agctcgccct gccccgccag
     4201 caaggcggca tgctcgaggt cgttgctgct gcctgcagaa cccggcgcca tttcgccatc
     4261 ggcaacggcg cggcgcgtca gggtcaccgc ggaaagcttg cccaggtagt tgcgcttgtt
     4321 gcgatcgcgc tccagcgcgc gcagggcctc cgcggccctg ggctcatccg gctgcagcgc
     4381 gagcaccgag agatagagct gggtggcacc atccatttcg ccgcgctttt gggcctgggc
     4441 ggctcgctgc atgcgctcgg cgacctgggc atcgatctgg ctgcgcgtct cagccaggcg
     4501 ctcgcggtat tcgcgcagct cgggcctgag gacggtgagg atctcccagc tcagcgcagc
     4561 atcggccagc cggccctgcc gggccatgct gagcgcgcgc tccttctggc tgcgttcgta
     4621 gcctgcggcg gatatctgag ccgcaggcgt cgattcagtc gccgcgggcc cggtgggcgg
     4681 cgcgccggcg cagccggcca gcatcgcagc gattgcggca ccggcgatgc agcaggggtg
     4741 ccaactgctc atggcgcatt gatcccgcgg ctgatctgca gcttggcctt gaaggcttcc
     4801 aggtcctgat cgccgaagat catcgtcctc cgcagcatca acggctgcgc gaagaaggca
     4861 ttgccgcccg gattcacggt gacggccagc tggtactgct gtcgcgccag cgcagccagc
     4921 acggtctcgt tggcgtcgcc ataggggtag gcgtagttcc tgatcttcac gcccagcctg
     4981 cgctccagca attcgcgcgg cgccttggcc tccatctcga tgtcctggcg ataacgctcg
     5041 tcgtcttcgc cagcggcacg ctcgatgagg ttgcgatgcg tcttggaatg ggcctcgata
     5101 tcgatcagcc ctgaagcggc cagctcctgc agttgggccc agctgaggcc ttcgcgcgca
     5161 ccgatgaaat cggtgtagac gaacagcgtg gcggggaaac catacttgcg cagcagcggg
     5221 aaggcgtagc ggtagaccga ctcgtagccg tcgtcgatcg tgatcacgac cgagcgcctg
     5281 ggcagcggct gccgcccttc c
//
LOCUS       DASUCY010000128         3401 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_789952, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000128 DASUCY010000000
VERSION     DASUCY010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789952"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1081)
                     /locus_tag="VFV25_02550"
     CDS             complement(<1..1081)
                     /locus_tag="VFV25_02550"
                     /inference="COORDINATES: protein motif:HMM:NF045284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HEY1392224.1"
                     /translation="MHAAVMPPVEAREDAGGLIEQLIERLKAKLPPDQVEEAAEFTRQ
                     YYGQVAPEDLAERSLGDLYGAALSHWHFARSFAGGAPKLRVYNPRLDEHGWQSTHTVV
                     EIVNDDMPFLVDSITMEVNRQGLTLHLIAHPVMKVRRDAEHRLVSLARRRDEPEGRFE
                     SLIHVEVDRRTDAARLAELHDGLLRILGDVRAAVEDWKPMRQSVIDTVAQLQRSAPPQ
                     SSADPAEDVAFLQWLADDNFTLLGQRDYDLVADSSGMDTLRVVPGSGLGILRAGSDTA
                     SASASFAALPAELRARAREPRALVLTKSNARATVHRPGYLDYVGVKRYDAQGRVSGER
                     RFLGLFTSTAYNTNPAEIPLLRRKVA"
     gene            complement(1192..1671)
                     /locus_tag="VFV25_02555"
     CDS             complement(1192..1671)
                     /locus_tag="VFV25_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY1392225.1"
                     /translation="MKSLLLVRHAKSSRDDPSLPDRERPLNERGLQDAPGMGKRLAKR
                     KLKPDLIVASPALRALTTAQLMADEIGYARKDIVVEERLYASSAGQLLAVIHALDKKL
                     SCVMLFGHNPEFTDLAHSLSGEIIDLPTCAVAEFRYAAWRDVGEVGPSAVTLETPKK"
     gene            complement(1668..1892)
                     /locus_tag="VFV25_02560"
     CDS             complement(1668..1892)
                     /locus_tag="VFV25_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013204699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392226.1"
                     /translation="MTNPHASLTLQLLDWIAERPRSYAELMEAWKTSCPRLSIWEDAC
                     TEGLVSWGPGREGLVSLTEKGQDLLRLRAP"
     gene            complement(1936..2448)
                     /locus_tag="VFV25_02565"
     CDS             complement(1936..2448)
                     /locus_tag="VFV25_02565"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEY1392227.1"
                     /translation="MKQSDLLLAAAGALTALCCGAARADDGIQAHARLIGYQEVPALA
                     SGATGTFQATLSPSSFSFVLSYSGLDASPTQAHIHFGQKSVNGGISVFLCSNLGNGPA
                     GTQPCPSPGGTVTGTITAASVIGPTGQDISPGQFEALIRAMRAGITYANVHSTKFPAG
                     EIRGQIKIDD"
     gene            2623..2925
                     /locus_tag="VFV25_02570"
     CDS             2623..2925
                     /locus_tag="VFV25_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392228.1"
                     /translation="MRYKIRIATFLATSSAYLLCAATPAVRSNGASAPQAQQVRCEKE
                     LKDYSEAMRFVRQNAGDRIGDRVAAGYIDEAQLQRVAQTSGPCAAAQLLHDKRAMH"
     gene            3085..>3401
                     /locus_tag="VFV25_02575"
     CDS             3085..>3401
                     /locus_tag="VFV25_02575"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY1392229.1"
                     /translation="MPKHHGSARLSALAATLAAFAALAPNAQAGEDSHHRRIEHVLLI
                     SVDGMHAADLERYIATKPASTFAKLTTHGVRYTQASTAKPSDSFPGMLALATGGTPLL
                     HGV"
BASE COUNT          520 a         1125 c         1191 g          565 t
ORIGIN      
        1 cggccacctt gcgccgcagc agcgggatct cggccggatt ggtgttgtag gcggtggacg
       61 tgaagagccc caggaagcgc cgctcgccgc tgacgcggcc ctgcgcgtcg tagcgcttga
      121 ctcccacata gtcgagatag ccggggcggt gcaccgtggc gcgcgcgttc gacttggtca
      181 gcaccagcgc gcgcggctcg cgggcgcggg cgcgcagctc ggccggcagc gcggcgaagg
      241 aggccgaggc gctggccgtg tcggagccgg cgcgcaggat gccgagcccg gagcccggca
      301 ccacgcgcag cgtgtccatg ccgctgctgt cggccacgag gtcgtagtcg cgctggccga
      361 gcagcgtgaa gttgtcgtcg gccagccact gcaggaaggc gacgtcctcg gcgggatcgg
      421 ccgaggactg gggtggcgcg ctgcgctgca gctgcgccac ggtgtcgatc acggactgtc
      481 gcatcggctt ccagtcttcg accgccgcgc gcacgtcgcc caggatgcgc agcagcccgt
      541 cgtgcagctc ggcgagcctg gccgcgtcgg tgcggcggtc gacctcgacg tggatcagcg
      601 actcgaagcg gccctcgggc tcgtcacgcc gtcgggccag ggagacgagg cggtgctcgg
      661 cgtcgcgcct caccttcatt acggggtgcg cgatcaggtg cagcgtgagc ccctggcggt
      721 tgacctccat cgtgatcgag tcgaccagga agggcatgtc gtcgttgacg atttccacca
      781 ccgtgtgggt cgactgccag ccgtgctcgt ccaggcgcgg gttgtagacg cgcagcttgg
      841 gcgccccgcc cgcgaagctg cgcgcgaaat gccagtgcga cagcgccgcg ccatagagat
      901 cgcccagcga gcgctcggcc aggtcctcgg gcgcgacctg gccatagtat tggcgcgtga
      961 attcggcggc ctcttccacc tggtcgggcg gtagcttggc cttcaggcgc tcgatcagct
     1021 gttcgatcag gccccctgca tcctcgcgag cctctactgg cggcatgaca gctgcgtgca
     1081 tggtggcctc ctgggttgga gtgcgccggc cctgggcgac gcggcgcggg cggagatcgg
     1141 actccgcagt atccgccaac ggccaggcga gttgaagtcg cttgcccagg actacttttt
     1201 gggtgtttcc agcgtcacgg cggacgggcc gacttcgccg acatcccgcc atgccgcata
     1261 gcggaactcg gcgacagcgc aggtgggcag gtcgatgatc tcgccggaca ggctgtgcgc
     1321 gaggtcggtg aactcggggt tgtggccgaa cagcatcacg cagctgagct tcttgtcgag
     1381 cgcgtggatg acggccagca gctggccggc actgctggcg tacagccgct cttcgaccac
     1441 gatgtccttg cgcgcgtagc cgatctcatc ggccatcagc tgtgccgtcg tcagcgcgcg
     1501 cagcgccggg ctggccacga tcaggtcggg cttgagcttg cgcttggcca ggcgcttgcc
     1561 catgcccggc gcgtcctgca ggccgcgctc gttcaagggg cgctcacggt cgggcaggct
     1621 cgggtcgtcg cggctcgact tggcgtggcg gacgagaagc agggacttca cggggctcgc
     1681 aggcgcagga gatcctggcc cttttccgtg aggctcacca agccttcacg tccggggccc
     1741 cagctgacca gcccctccgt gcatgcgtcc tcccaaatcg acagcctggg gcagctcgtc
     1801 ttccaggctt ccatgagctc ggcatagctg cgcggccgct cggcgatcca atcgagcagc
     1861 tgaagggtga gtgatgcatg cgggttggtc aacaaaggct cctggcgaga ccgcgggctg
     1921 ccgtgcaacg gcgattcagt cgtcgatctt gatttgtccg cgtatctccc cggccgggaa
     1981 cttggtggaa tgcacattgg cataggtgat gccggcgcgc atcgcgcgta tcagcgcctc
     2041 gaactggccg ggcgagatgt cctggccggt ggggccgatc acgctggccg cggtgatggt
     2101 gccggtgacc gtgccgccgg ggctggggca tggctgggtg ccggccggcc cattgcccag
     2161 gttcgagcac aggaagacgc tgatgccccc attcacgctc ttctggccga agtggatatg
     2221 cgcctgggtg gggctggcgt ccaggccact gtaggaaagc acgaagctga aggagctggg
     2281 cgaaagcgtt gcctggaagg tcccggtggc tcccgaggcg agggccggta cctcctggta
     2341 gccgatcagg cgggcatgcg cctgtatgcc gtcgtctgca cgggccgcgc cgcagcacaa
     2401 ggcggtgagc gcacccgccg ccgcgagaag aagatctgat tgcttcatcg tgatcctcca
     2461 tgggtgagcg gccgctgtgc gcggcctcca tcggatacgg ctggattgaa tccgcgaact
     2521 tctaacttag tcgcaaggcg agggctattc aacgtcggcg ggcagcgcgt catatcgagg
     2581 tcatgcgcgc caccaagaat cgctggctcc gaaggagcag tcatgcggta caaaattcga
     2641 atcgccactt tccttgcgac ttcatcggct tacctgctgt gcgcagcgac gccggccgtc
     2701 cgctcgaacg gcgccagcgc gccccaggcc cagcaggtgc gctgcgagaa ggagctgaag
     2761 gactattccg aggccatgcg cttcgtgcgc cagaacgcgg gcgaccggat cggtgatcgc
     2821 gtcgcggccg gctatatcga cgaagcgcaa ttgcagcgcg tcgcccaaac ctcggggcct
     2881 tgcgcagccg cgcagctgct gcacgacaag cgcgcgatgc attgaggggc cccgcgtatt
     2941 ggggggtagg gccgcaacgg gtgtcgcgag actgtcattg accgcgccta gattgccagc
     3001 ggcgagcgcc tcgcagcctc cggcgcccgc aaacgccggt gcaatcccgc gcgggcggtc
     3061 gacaaccaga ttcggagaga tcccatgccc aagcaccatg gcagcgcgcg cttgtcagcg
     3121 ctcgccgcaa cgctcgccgc cttcgcggca cttgctccca atgcgcaggc cggcgaagac
     3181 tcgcaccacc gtcgtatcga gcatgtgctg ctgatcagcg tggacggcat gcatgccgcc
     3241 gatctggagc gatacatcgc caccaagccg gcttcgacct tcgccaagct gaccacgcat
     3301 ggcgtgcgct acacccaggc ctcgaccgcc aagccttccg actccttccc cggaatgctt
     3361 gccctggcca ccggcggcac gccgctcttg cacggggtgt t
//
LOCUS       DASUCY010000129         2767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_532981, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000129 DASUCY010000000
VERSION     DASUCY010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532981"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="VFV25_02580"
     CDS             complement(<1..740)
                     /locus_tag="VFV25_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY1392230.1"
                     /translation="MTDRCPEQALAEFAARLDFAEIPPAVLRRAEDLMLDWFGSALAG
                     KNARPVETLTHFLESMGPGEGPSELLIHRRRSSPLIAAMVNAAASHFAEQDDVHNGSV
                     FHPAAVVFPAALAVAQALGRSGAELLASVVAGYEVGIRIGEFLGRSHYKVFHTTGTAG
                     TFAAAAAAGRLLRLTPEQMQHALGSAGTQSAGLWEFLRDAADSKQLHVAHAAGAGLTS
                     AYLAQDGFTGARRILEGPQGLAAGTSKDA"
     gene            complement(737..1771)
                     /locus_tag="VFV25_02585"
     CDS             complement(737..1771)
                     /locus_tag="VFV25_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY1392231.1"
                     /translation="MKFLIRAGLSALLGALALAGAGAALAPPAPIVIKFSHVVAPDTP
                     KGRGADSFKKLAEERTKGRVKVEVYPNSQLYKDKEELEALQLGSVQMLAPSLSKFGPL
                     GVREFEAFDLPYLFDNYDELHKVTRGPVGEALLKRLQPRGIVGLAYWDNGFKEFTANK
                     PLRRPEDFKGLKMRIQSSKVLDAQMRSVGAVPQVLAFSEVYQALQSGVVDGTENTPSN
                     IYTQKTHEVQKVLTLTHHGYIGYAVVVNKRFWDGLPPDIRSTLEGAMKDATQYADELS
                     KQENDDALEAMRKSGKIQLVEPTADEKKVLKKAMLAVHREMESRVGRDTEQQFYKATG
                     FDPNSFLSTK"
     gene            complement(1832..>2767)
                     /locus_tag="VFV25_02590"
     CDS             complement(1832..>2767)
                     /locus_tag="VFV25_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY1392232.1"
                     /translation="QADVLVQNLAPGAAARLGLSFEALHDRHPRLIVCDISGYGDDPA
                     QPGPYRDKKAYDLLIQSESGFLSITGSPGEPAKAGCSIADIAAGMYAYTNVLAALIER
                     GKTGRGRRIDVSMLESMVEWMGYPLYYSFDGATPPPRAGAAHATIYPYGPFPTGDGHT
                     VMLGLQNEREWQVFCQKVLQQPELIADERFSTNSRRTAARQALREIIVQAFAGLTADQ
                     VVARLDEAQIANARMNDMREVWEHPQLKARRRWVQVGTPSGTVPALLPPGTPDEPGAS
                     RMDAVPALGQHTEAILAELGYEPADIAGLREKRAI"
BASE COUNT          398 a          934 c          960 g          475 t
ORIGIN      
        1 gcgtctttcg aggtgccggc ggcgagaccc tgaggcccct ccaggatgcg gcgcgcgccg
       61 gtgaagccgt cctgggccag ataggcggaa gtcagcccag ccccggcggc atgtgccaca
      121 tgcagctgct tggaatcggc cgcgtcgcgc aggaactccc acagccccgc cgactgcgtg
      181 cccgctgagc caagcgcgtg ctgcatttgc tcgggcgtca ggcgcagcag ccgccctgcc
      241 gccgccgcgg cggcaaaagt gcctgcggtg ccggtggtgt ggaagacctt gtaatgcgag
      301 cgcccgagga attcgccgat gcgaatgccg acctcgtagc cggccaccac gcttgccagc
      361 aactcggcgc cgctgcggcc cagagcctgt gccaccgcga gcgctgcagg gaagaccacc
      421 gcggccgggt ggaagaccga gccgttgtgc acgtcgtcct gctccgcgaa atgtgaagcc
      481 gccgcattga ccatcgccgc gatcagcggg ctgctgcgcc ggcggtggat cagcagctcg
      541 ctcgggccct cgcccggccc catgctctcg aggaagtgcg tgagcgtctc aaccggccgc
      601 gcattcttgc cggccagcgc cgagccgaac cagtccagca tcaggtcttc ggcgcggcgc
      661 agcaccgcgg gcgggatctc cgcgaagtcc agccgcgcgg cgaattccgc cagggcttgt
      721 tcggggcagc ggtcggtcat ttggtgctca ggaaactgtt ggggtcgaag ccggtggcct
      781 tatagaactg ctgctcggtg tcgcggccga cgcggctctc catctcgcga tgaacggcca
      841 gcatcgcttt cttcaagacc ttcttctcgt cggcggtcgg ctcgacgagc tggatcttgc
      901 cgctcttgcg catggcttcc agcgcgtcgt cgttctcctg cttggagagc tcgtcggcgt
      961 attgggtcgc gtccttcatc gcgccttcga gcgtgctgcg gatgtcgggc ggcaggccgt
     1021 cccagaacct cttgttgacc accaccgcat agccgatata gccgtggtgc gtcagcgtga
     1081 ggaccttctg cacctcgtgg gtcttctgcg tgtagatgtt cgacggcgtg ttctcggtgc
     1141 cgtcgaccac gccgctttgc agcgcctggt agacctcgga gaaggccagc acctgcggga
     1201 ccgcgccgac cgagcgcatc tgcgcatcga gcaccttgga cgactggatg cgcatcttca
     1261 ggcccttgaa gtcctcgggg cggcgcagcg gcttgttggc ggtgaactcc ttgaagccgt
     1321 tgtcccagta ggccaggccg acgatgccgc gcggctgcag gcgcttgagc aaggcctcgc
     1381 cgaccggccc gcgcgtcact ttgtgcagct cgtcatagtt gtcgaagaga taaggcaggt
     1441 cgaaagcctc gaactcccgc acgcctagcg gcccgaactt ggacagcgag ggcgccagca
     1501 tctgcaccga gccgagctgc agcgcctcga gctcctcctt gtccttgtag agctggctgt
     1561 tgggatagac ctcgactttc acgcggccct tggtgcgctc ctcggccagc ttcttgaagg
     1621 agtcggcgcc ccggcccttg ggcgtgtcgg gcgccaccac gtggctgaac tttatgacga
     1681 tgggcgccgg cggggccagc gccgcgccgg cgcccgcgag tgccagggcc ccgagcaatg
     1741 cggacagtcc tgcgcggatg aggaacttca tgcttgtctc cttggtggcg atgccactcg
     1801 tgttgtcgaa agaacgctgg atcgcccaaa gctagatcgc ccgcttctcg cgcaggccgg
     1861 cgatatcggc cggctcatag cccagctcgg ccaggatggc ctcggtgtgc tggcccaggg
     1921 ccggcaccgc gtccatgcgc gatgcgccgg gctcgtcggg cgtgccaggc ggcagcagcg
     1981 cgggcaccgt cccgctgggc gtgccgacct gcacccagcg ccggcgcgcc ttgagctggg
     2041 gatgctccca gacctcgcgc atgtcgttca tgcgcgcgtt ggcgatctgc gcctcgtcca
     2101 gccgcgccac cacctggtca gccgtgaggc cggcgaaggc ctgcacgatg atctcgcgca
     2161 gcgcctggcg cgctgccgtg cgccggctgt tggtggagaa gcgctcgtcg gcgatcagct
     2221 ccggctgctg cagcaccttc tggcagaaca cctgccattc gcgttcgttc tgcaggccca
     2281 gcatcacggt gtggccgtcg ccggtcggga aggggccgta gggatagatg gtggcgtgcg
     2341 cggcgccggc gcgcggcggc ggggtggcgc cgtcgaagga gtagtacagc ggatagccca
     2401 tccactccac catgctctcc agcatcgaca cgtcgatgcg ccgcccgcgg ccggtcttgc
     2461 cgcgctcgat cagcgcggcc agcacattgg tgtaggcata catgccggcc gcgatgtcgg
     2521 cgatcgagca gcccgccttg gcgggctcgc cgggcgagcc ggtgatggag aggaagcccg
     2581 actcgctctg gatcaagagg tcgtaggcct tcttgtcgcg atacggcccg ggctgcgcgg
     2641 gatcgtcgcc atagccagag atgtcgcaga cgatgaggcg cgggtgcctg tcgtgcaagg
     2701 cctcgaagga caggccgagc cgtgccgcgg cgccgggcgc gaggttttgc accagcacgt
     2761 cggcctg
//
LOCUS       DASUCY010000130         3498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_372374, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000130 DASUCY010000000
VERSION     DASUCY010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372374"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..806)
                     /gene="fabG"
                     /locus_tag="VFV25_02595"
     CDS             complement(60..806)
                     /gene="fabG"
                     /locus_tag="VFV25_02595"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEY1392233.1"
                     /translation="MSAVKFEGQIALVTGASRGIGRAIALTLAQRGLKVLGTATTEAG
                     AAAIGEALAPYGGRGLVLNVTDAASLDAAIDGIVKEFGALHVLVNNAGITRDNLSMRM
                     KDEEWDAVLDTNLKAVFRASRAVMRTMMKQRYGRIINITSVVGASGNAGQANYAAAKA
                     GLAGMTRSLARELGSRGITVNCVAPGFIATDMTDALPEAQKTALLAQIPLGRLGEPSD
                     IAEAVAYLASPQAGYITGTELHVNGGMYMN"
     gene            complement(803..1744)
                     /gene="fabD"
                     /locus_tag="VFV25_02600"
     CDS             complement(803..1744)
                     /gene="fabD"
                     /locus_tag="VFV25_02600"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEY1392234.1"
                     /translation="MTTSFAFVFPGQGSQSVGMLDAWGDHPAVRATLAEASEALGADI
                     AQLIAQGPKESLDLTTNTQPVMLTAGIACYRAWMAETGLAPAAVAGHSLGEYAALVAA
                     GALQLGDALPLVRFRAQAMQEAVPVGAGGMAAILGLDAEAVRAGCAEAAAASGEVVEA
                     VNFNDPKQTVIAGTKAGVEKACEVLKAKGAKRALPLPVSAPFHSSLMKPAAERLREKL
                     AATTFAAPSIPVVNNIDVKVESDPAAIRDALYRQAFGPVRWVEIVLALKARGLGHVLE
                     CGPGKVLAGMAKRIDGELVSASVFDPASLAEAKEILG"
     gene            complement(1798..2775)
                     /locus_tag="VFV25_02605"
     CDS             complement(1798..2775)
                     /locus_tag="VFV25_02605"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEY1392235.1"
                     /translation="MPRYSRITGTGSYLPPKRVTNEELAAELAQRGIETSDQWIVERT
                     GIRARHFAAPDVTSSDLAAAAARQALEAAGRKAEEIDLILVATSTPDMIFPSAACLVQ
                     QKLGIQGCPAFDVQAVCAGFVYALTVADSMIRTGAASKALVIGAEVFSRILDFNDRTT
                     CVLFGDGAGAVVLEASGQPGLLASELHADGRHVDILCTPGTVSGGKVLGDPLLKMDGQ
                     AVFKLAVGVLEQVARGVLAKAGRLESDIDWLIPHQANIRIMQSTAKKLKLPLDKLIVT
                     VDEHGNTSAASIPLALDHAVRGGKVRKGDTVMLEGVGGGFTWGAVLLDF"
     gene            complement(2784..>3498)
                     /gene="plsX"
                     /locus_tag="VFV25_02610"
     CDS             complement(2784..>3498)
                     /gene="plsX"
                     /locus_tag="VFV25_02610"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEY1392236.1"
                     /translation="DGIDRPAIATVLPNQLDKHTTVLDLGANVDCTAEHLLQFAVMGS
                     ALVAAVEGAENPSVGLLNIGEEAIKGSEAIKKAGELLRFAANGGHLNFYGNVEGNDIF
                     KGTVDIVVCDGFVGNVALKTAEGLASMFSSFIRQEFTRNFFTKMAAIVALPVLNHFKN
                     RIDHRRYNGAALLGLRGLVFKSHGSADAFAFEQALNRAYDAARNRLLDRVHERILETL
                     QALPTEATDAATATVAQAA"
BASE COUNT          563 a         1178 c         1166 g          591 t
ORIGIN      
        1 acggttgccg cagcccggac gggcctcaac cggtccgggc gccacccaag gggcgcccct
       61 cagttcatgt acatgccgcc gttgacgtgt agttccgtgc ccgtgatgta gcccgcctgc
      121 ggcgaggcca gataagccac ggcctccgcg atgtcggacg gctcgcccag gcgcccaagc
      181 ggaatctgtg ccagcagcgc ggtcttctgg gcctcgggca aggcatcggt catgtcggtg
      241 gcgatgaagc caggggccac gcaattgacc gtgatgccgc ggctgccgag ctcgcgcgcc
      301 aatgaacggg tcatgccggc cagccccgcc ttggccgcgg cgtagttcgc ctggcccgca
      361 ttgccgctgg cgccgaccac ggaggtgatg ttgatgatgc ggccgtagcg ctgcttcatc
      421 atcgtgcgca tcacggcgcg gctggcgcgg aagacggcct tgaggttggt gtccagcacg
      481 gcatcccatt cctcgtcctt catgcgcatg gacaggttgt cgcgcgtgat gccggcgttg
      541 ttgaccagca catgcagggc accgaattcc ttgacgatgc catcgatcgc agcatcgagc
      601 gaagcggcgt cggtgacgtt gagcaccagc ccgcgcccgc cgtacggcgc cagcgcctcg
      661 ccgatcgcgg cggcaccggc ttcggtggtg gccgttccca gtaccttcag cccgcgctgt
      721 gccagcgtca aggcgatggc gcggccgatg ccgcggctgg cgccggtgac gagggcgatc
      781 tggccttcaa atttcaccgc gctcatccca aaatttcctt tgcttcggcc aggctggccg
      841 gatcgaacac gctcgcgctc accagttccc cgtcgatgcg cttggccatg ccggccagca
      901 ccttgccggg cccgcattcc agtacatggc ccaggccgcg cgccttcaga gccagaacga
      961 tctcgaccca gcgcaccggg ccgaaggcct ggcgatagag cgcgtcgcgg atcgcggcgg
     1021 gatcgctctc gaccttgacg tcgatgttgt tgaccacagg aatcgacggc gccgcaaaag
     1081 tcgtggccgc cagcttctcg cgcaggcgct cggccgccgg cttcatcagg ctggagtgga
     1141 aaggcgccga cacaggcagc ggcagcgcgc gcttggcacc cttggccttg aggacttcgc
     1201 aggccttctc gacgccggcc ttggtgcctg cgatgacggt ctgcttgggg tcgttgaaat
     1261 tgacggcttc cacgacctcg ccactggccg ccgccgcttc ggcgcagccg gcgcgcacgg
     1321 cttcggcgtc cagccccagg atggccgcca tgccgcccgc gcccaccggc acggcctctt
     1381 gcatcgcctg ggcccggaac ctgaccagcg gcagagcgtc gccgagctgc agcgcgcctg
     1441 cggcgaccag tgccgcgtat tcacccagcg aatggcctgc caccgccgcc ggcgccaggc
     1501 cggtttcggc catccaggcg cgatagcagg cgatgccggc tgtcagcatc accggctggg
     1561 tattggtggt gagatccagg ctttccttgg gcccctgcgc gatcagctgc gcgatgtcgg
     1621 cgcccagcgc ctcgctggct tcggccagcg tcgcgcgcac cgcgggatgg tcgccccagg
     1681 cgtcgagcat gccgaccgac tgcgagcctt gaccgggaaa gacgaaagcg aatgaggtcg
     1741 tcattgcgca aaccttcttg taggaaacgc gaaaggcacg agcgaaccgg gtcggactca
     1801 gaagtccagt agcacggcgc cccaggtgaa gccgccgccc acgccctcga gcatcacggt
     1861 atcgcccttg cgaaccttgc cgccgcgcac ggcatggtcc agcgccagcg gaatcgacgc
     1921 ggccgaggtg ttgccatgct cgtccaccgt gacgatgagc ttgtccagcg gcagcttcag
     1981 cttcttggcc gtgctctgca tgatgcgtat gttggcctgg tgcgggatca gccagtcgat
     2041 gtcgctctcc agccgcccgg ccttggcgag cacaccgcgc gcgacttgct cgagcacgcc
     2101 gaccgcgagc ttgaacacgg cctggccgtc catcttgagc agaggatcgc ccagcacctt
     2161 gccgcccgag acggtccccg gcgtgcagag gatgtcgacg tgacgcccgt cagcgtgcag
     2221 ctcgctggcc aggaggcccg gctgcccgga ggcttcgagc accacggcac cggcgccgtc
     2281 gccgaagagc acgcaggtgg tgcggtcgtt gaagtccagg atgcgcgaaa acacttcagc
     2341 gccgatcacc agggccttgc tcgcggcgcc ggtgcggatc atcgagtcgg ccaccgtcag
     2401 cgcgtagacg aagcctgcgc agaccgcctg cacgtcgaag gccgggcagc cttgaatccc
     2461 caacttttgc tgcacgaggc aggcggccga ggggaagatc atgtcgggcg tggaggtggc
     2521 gaccaggatc aggtcgatct cctcggcctt gcgcccagcc gcctccaggg cctgcctggc
     2581 ggcagcggcg gcgagatcgc tggacgtgac gtcaggcgcc gcgaaatggc gcgcccggat
     2641 gccggtgcgc tcgacgatcc actggtcgga agtctcgatg ccgcgctgcg caagctcggc
     2701 ggcgagctcc tcattagtga ctcgcttcgg cggcagatag ctgccggtgc cggtgatccg
     2761 ggaataacgg ggcagggatg aggtcatgca gcttgggcca cggtagcggt ggcggcatcg
     2821 gttgcctcag tcggcagcgc ttgcagggtc tcgaggatgc gctcatgcac ccggtcgagc
     2881 agtcgattgc gggcggcatc atacgcccga ttcaaggcct gctcgaacgc gaaggcgtca
     2941 gccgagccgt ggctcttgaa cacgaggccg cgcaggccga gcagggccgc gccgttgtag
     3001 cgccggtggt caatgcgatt tttgaagtga tttagcaccg gaagggcgac gatcgcggcc
     3061 attttggtga agaagttgcg agtgaactcc tgcctgatga agctggagaa catgctcgcc
     3121 aagccttcgg ccgtctttag cgccacattg ccgacgaagc cgtcgcaaac gacgatgtcg
     3181 accgtgccct tgaagatatc gttgccctcg acattgccat agaaattgag atggccgcca
     3241 ttggcagcaa atctcagcag ttcgccggcc tttttgatcg cttcgctgcc tttgatggct
     3301 tcttcgccga tgttgagcaa acccacgctg gggttctcgg ccccctcgac cgccgccacc
     3361 agcgcactgc ccatcacggc gaactggagc aaatgctcgg ccgtgcaatc cacattggcc
     3421 cccaggtcca gcaccgtggt gtgcttgtcg agctgattgg gcagcacggt ggcaatcgcc
     3481 gggcggtcga tgccgtcc
//
LOCUS       DASUCY010000131         7228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_227739, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000131 DASUCY010000000
VERSION     DASUCY010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 7228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7228
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227739"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /locus_tag="VFV25_02615"
     CDS             <1..1080
                     /locus_tag="VFV25_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY1392237.1"
                     /translation="DESLTGQYLAHKLRIPVPRKRHALADGTDPLTLKIVNARGNNLK
                     NVTAEIPVGLFSCVTGVSGSGKSTLVNDTLYAAVARRLYQSHLDPAPHDEIEGLDAFD
                     KVINVDQSPIGRTPRSNPATYTGLFTPIRELFAEVPTARERGYGAGRFSFNVAGGRCE
                     ACQGDGVLKVEMHFLPDVYVPCDVCHGKRYNRETLEILYKGKNISEVLDLTVEDAHEF
                     FSAVPSIARKLQTLLDVGLGYIHLGQSATTLSGGEAQRVKLALELSKRDTGRTLYILD
                     EPTTGLHFHDIALLLKVLHQLRDHGNTIVVIEHNLDVIKTADWIIDMGPEGGAGGGQV
                     LASGTPEDIAAHPASYTGQYLKPLL"
     gene            1138..1749
                     /locus_tag="VFV25_02620"
     CDS             1138..1749
                     /locus_tag="VFV25_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HEY1392238.1"
                     /translation="MSLSIDYYLAPQSPWTYLGHQRFWALAKAHGATIRVLPVDLGGR
                     VFPVSGGLPLAKRAPQRQAYRLLELGRHSQWLGLALNPQPKYFPVASDDAARLIIAVD
                     MKDGSEAAMGITDALLRAVWVQERNIADEATLAALLAEQGLPAQRLEDAHAQAAHERY
                     EMDSQLAIQAGVFGAPSYVIEGEIFWGQDRLDFVERRLQLARR"
     gene            1885..4701
                     /locus_tag="VFV25_02625"
     CDS             1885..4701
                     /locus_tag="VFV25_02625"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY1392239.1"
                     /translation="MPPTVPSHDAEAQRLAVLRSLGVVDSSQSEPSLDALAESAARLL
                     GCPAGFITLIDSDQQWIKAGYGLQTATQTLPREISFCTHLVSSQADVLEVPDASLDAR
                     FADNPFVAGEPHVRFYAGAPLPVDGCNVGTLCVIDFRPRRLSEDDRRSLRRLAQAASE
                     LLEHRRLLRRQGAEQQRLRDLARASGDWMWETDEQGRYTWISSAFDALTGLPGASLLG
                     QPMADAPLLDEQGRSAQGSLHQLLAHGQAFVRALSEKTTPRGRLIVSRSAVPVRNGEG
                     QLVAWRGTTRDTTQRITLLAEARARDAVLRRFSAQIPGLVFQFRRAPGGGLSFPYLSE
                     RVEDLLGVTADRVAADVEADPSHAFRLVVDEDLPGLKAAIAASELKLEPLHHEYRVRL
                     RDGRLRWLETRATPERLDNGDTLWHAFSADVTARKQTELALRHSEERWAMAASAAGIG
                     IAQLDLLNGTLRLDRQACLNHGLPFPHGALSLADWLAQLDPRDRPAAEAGLRQAIDAH
                     GALEARYRIHRNVDGAERWLEILARAQYDTRGLAVGMIGTCRDVTEAQLAEQLKRDKA
                     EAERASRAKTRFLSRMSHELRTPLNGILGFVQLMSMDRQNPLPPAQQARLDSVQWASR
                     HLLGLINEVLDLSRIEREDFHLRLRPVELGGSLAACLALLLPLAQARGVTLAAAPAGQ
                     FWVRGDAHALEQVLMNLLSNAIKYNREDGRVWLQARFEADRLRLEVHDTGAGLDAEQR
                     GQLFQPFVRLSREAARIEGSGLGLFIARQLAEAMGGSLDAHSEAGVGSVFRLELVRCE
                     PAEGSGGDETQPSSRTAQATRHVVYVEDEPLNVVLLQELFRARAHWRLHVATSGSQGL
                     ELARATPADLLLIDMNLPDMDGLELLRQLRADPATRELRCVALSADALPEQIARARES
                     GFDNYWTKPFDVTRLLGELDRLLA"
     gene            complement(4777..5199)
                     /locus_tag="VFV25_02630"
     CDS             complement(4777..5199)
                     /locus_tag="VFV25_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HEY1392240.1"
                     /translation="MATAKKAPAKKAAAKKAPAKKPAAKKAAPAKKAAAKKAPAKKAA
                     AKKAPAKKAAPAKKRTPNAAFMKALTPSAALAAIVGDKPLPRTEVTKKIWDYIKKNKL
                     QDSVNKRMINADAKLKEIFKKAQASMFEMTKLVNSHLK"
     gene            5496..5852
                     /locus_tag="VFV25_02635"
     CDS             5496..5852
                     /locus_tag="VFV25_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEY1392241.1"
                     /translation="MKTLLRWLLLAAALLLVAHLYPGVEVKSFGSAMIAAAVLGLFNA
                     VLRPILVVLTLPVTLVTLGLFLFVINALMFYGAAYVLKGFSVHGFVAALIGSLLYSAC
                     GVVIDAALERLFVKGD"
     gene            complement(5899..>7228)
                     /locus_tag="VFV25_02640"
     CDS             complement(5899..>7228)
                     /locus_tag="VFV25_02640"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY1392242.1"
                     /translation="PLVAASRARGNLTQELSDHFAWQPFLVTDKTINAFALPGGYVGV
                     HLGLISMTATSDELASVLAHEMSHVTQRHIARMIGNEQRVSMVGIASMILGILAAARS
                     PQAGEALIVGGQAVTAQGQLNFSRDREREADRVGFGVLQGAGYQPAGMAGMFERLAAA
                     SRLTDSGDFPYLRSHPLTTERIGEARARLGVNADENPAMSKNLLEHAAYRGRARALMD
                     PRAAALARVAANEGGAATSKAERLTSVYAAALAATKLKEWPRADEMLAQARELAQGDA
                     GAARAVALLQAESSIERGDAARAAAALAPHAGDGTRPVAMLQARLALLPGTPRAQLRR
                     SVDELQTWVALHPKDALAWTYAAQAWERLDEPLRAVRAQAEVRVAIGDYDGAVDRLHA
                     GQTLARKSAARDSIEASVIEARMRDVQALRRREMADERGNNKDKSEEPKS"
BASE COUNT         1083 a         2535 c         2434 g         1176 t
ORIGIN      
        1 gacgaatcgc tgaccgggca atacctggcg cacaagctgc gcatcccggt gccgcgcaag
       61 cggcatgcgc tggccgacgg caccgacccg ctgacgctca agatcgtgaa tgcgcgcggc
      121 aacaacctca agaacgtgac ggccgagatc ccggtcggcc tcttcagctg cgtgaccggg
      181 gtttcgggct cgggcaagtc cacgctggtc aacgatacgc tctacgccgc ggtggcgcgc
      241 cggctctatc agagccatct cgatccggcg ccgcatgacg agatcgaggg gctggacgcc
      301 ttcgacaagg tcatcaacgt cgaccagtcg cccatcggcc gcacgccgcg cagcaatccg
      361 gccacctaca cgggtctctt cacgccgatc cgcgagctgt tcgccgaggt gccgacggcg
      421 cgcgagcgcg gctatggtgc cgggcgcttc tccttcaacg tcgccggcgg gcgctgcgag
      481 gcctgccagg gcgacggcgt gctgaaggtg gagatgcatt tcctgcctga cgtctacgtg
      541 ccctgcgacg tctgccacgg caagcgctac aaccgcgaga cgctggagat cctctacaag
      601 ggcaagaaca tctccgaggt gctggacctg acggtggagg acgcccacga gttcttcagc
      661 gccgtgccca gcatcgcgcg caagctgcag accctgctcg acgtgggcct gggctatatc
      721 cacctgggcc agagcgccac aacgctgtcg ggtggcgagg cacagcgcgt caagctggcg
      781 ctggagctgt ccaagcgcga caccggccgc accctctaca tcctcgacga gccgaccacc
      841 ggcctgcatt tccacgacat cgcgctgctg ctcaaggtgc tgcaccagct gcgcgaccac
      901 ggcaacacca tcgtcgtgat cgagcacaac ctggacgtca tcaaaacggc cgactggatc
      961 attgacatgg gccccgaggg cggggccggc ggcggccagg tgctggccag cggcacgccg
     1021 gaagacatcg cggcccatcc ggccagctac accgggcaat acctcaaacc cctgctgtga
     1081 cggctggcta tcagtctcgc tagactgcgc cgcagaccca acagcctagg agccgaggtg
     1141 agcctgagca tcgactacta cctggcgccg cagtcgccct ggacctatct cggccaccag
     1201 cgcttctggg cactggccaa ggcgcacggc gcgacgatac gcgtgctgcc ggtggacctg
     1261 ggcggccgcg tctttcccgt ctccggcggg ctgccgctgg ccaagcgggc accgcagcgc
     1321 caggcctatc gcctgctcga gctcgggcgc cacagccaat ggctggggct ggcgctgaac
     1381 cctcagccga aatactttcc tgtggcttcc gacgacgcgg cgcgcctgat catcgcggtc
     1441 gacatgaagg acggcagcga agcggccatg ggcatcaccg atgccctgct gcgcgcggtg
     1501 tgggtgcaag agcgcaacat cgccgacgaa gccacgctgg ccgccctgct cgccgaacaa
     1561 ggcttgcctg cgcagcgtct cgaagacgcc catgcccagg ccgcgcacga gcgctacgag
     1621 atggattcgc agctcgcgat ccaggccggc gtcttcggcg cgcccagcta tgtgatcgag
     1681 ggcgagatct tctggggcca ggaccggctg gacttcgtcg agcgccggct gcagcttgca
     1741 cggcgctaac agcgccttgg tgttccgcga cttttcgcac ggagtgcagg gactggggca
     1801 gatcccagct gccgtgcccg ccggccggcg ggcacaatgc gccggcctcc gccgcgccgg
     1861 cttcggcgtt tccccgctcc gcccatgccc cctacggtcc cgtcgcacga cgcagaagcc
     1921 cagcgcctgg ccgtattgcg cagcctcggg gtggtcgaca gcagccaatc cgagcccagc
     1981 ctcgacgcgc tggccgaatc tgcggcccgt ttgctcggct gccccgccgg cttcattacg
     2041 ctgatcgaca gcgatcagca atggatcaag gcgggctacg gcctgcaaac ggcaacgcag
     2101 acactgccgc gcgagatcag cttctgcacc catctcgtgt cgtcccaggc cgacgtgctg
     2161 gaagtgccgg atgccagtct cgatgcacgc tttgccgaca accctttcgt cgccggcgaa
     2221 ccccatgtgc gcttctatgc cggcgcgccg ttgccggtgg acggctgcaa cgtcggcacg
     2281 ctgtgcgtga tcgattttcg cccgcgccgc ctcagcgagg acgatcgccg ttcgctgcgc
     2341 cggctggcgc aggcagcctc cgagctgctg gagcatcgcc gcctgctgcg ccggcaaggc
     2401 gccgagcagc agcgcctgcg ggacctcgcc cgcgcttccg gcgactggat gtgggagacc
     2461 gatgagcagg ggcgctacac ctggatttcc agtgccttcg acgccctcac cggcttgccg
     2521 ggcgcgagcc tgctagggca gcccatggcc gatgcccccc tgctcgacga gcaaggccgc
     2581 tccgcgcagg gcagcctgca tcaattgctg gcgcacggcc aggccttcgt gcgcgccctc
     2641 agcgagaaga ccacgccgcg cggtcgcctc atcgtttcgc gcagcgccgt gccggtgcgc
     2701 aacggcgagg gccagctggt ggcctggcgc ggcacgacgc gcgacaccac gcagcgcatc
     2761 accctgctgg ccgaagcgcg cgcccgcgat gcggtgctgc gccgcttcag cgcccagata
     2821 ccgggcctcg tgttccagtt ccggcgcgcg ccgggcgggg gcttgagctt tccctacctg
     2881 agcgaacgag tggaggatct gctgggagtg acggcggaca gggtggcggc cgatgtggag
     2941 gccgatccga gccatgcctt ccgcctcgtc gtcgatgaag acctgcccgg cctcaaggcc
     3001 gccatcgccg catccgagct caagctcgag cccttgcacc atgaataccg cgtgcgcctg
     3061 cgcgacggcc ggctgcgctg gctggaaacg cgggccaccc cggagcggct ggacaacggt
     3121 gacacgctgt ggcacgcctt ttcggccgac gtcaccgcgc gcaaacagac cgagctggcc
     3181 ctgcgccact cggaagagcg ctgggccatg gcggcctccg cggcgggcat cggcatcgcc
     3241 cagctcgatc tgctcaacgg cacgctgcgg ctcgatcgcc aggcctgtct gaaccacggc
     3301 ctgccgttcc cgcacggcgc gctcagcctc gccgattggc tggcacagct cgatccgcgc
     3361 gatcgccccg cagccgaggc gggtttgcgc caggccatcg acgcgcacgg ggcgctggaa
     3421 gcgcgctatc gcatccaccg caacgtggac ggcgccgagc gctggctgga gatcctggcg
     3481 cgcgcccaat acgacacgcg aggactggcc gtcggaatga tcggcacctg ccgcgacgtc
     3541 accgaggccc agctcgccga gcagctcaag cgcgacaagg ccgaggccga gcgcgccagc
     3601 cgtgccaaga cccgctttct ctcgcgcatg agccacgagc tgcgcacgcc gctcaacggg
     3661 atcctcggct tcgttcagct gatgagcatg gaccgccaga atcccttgcc gccagcgcag
     3721 caggccaggc tggacagcgt gcaatgggcc agccgccatc tgctggggct gatcaacgaa
     3781 gtgctggatc tctcgcgcat cgagcgcgag gacttccatc tgcggctgcg gccggtggag
     3841 ctgggcggct ccctagcggc ctgcctggcc ctgctgctgc ccttggccca ggcacgcggc
     3901 gtcacgcttg cggcggcgcc agccggccag ttctgggtgc gcggcgacgc gcatgcgctg
     3961 gagcaggtgc tgatgaacct gctgtccaac gcgatcaagt acaaccgcga agatgggcga
     4021 gtctggctgc aggcgcgatt cgaggccgat cggctacggc tcgaagtcca cgacaccggc
     4081 gccggcctgg acgccgagca gcgcggccag ctgttccagc ccttcgtgcg gctgagccgg
     4141 gaagccgcgc gcatcgaagg cagcggcctg ggccttttca tcgcgcgcca gctcgccgag
     4201 gcgatggggg gcagtctcga cgcgcacagc gaagccggtg tcggctcggt gtttcgcctg
     4261 gagctcgtgc gctgcgagcc ggcagaagga tccggcggcg acgagacgca gccttcaagc
     4321 agaactgcac aggccacccg ccacgtcgtc tatgtcgagg acgagccgct caacgtcgtg
     4381 ctgctccagg agctgtttcg tgcccgcgcg cattggcggt tgcatgtcgc gacgagcggc
     4441 tcgcagggcc tggagctggc ccgcgccaca ccggccgacc tgctgctgat cgacatgaac
     4501 ctgcccgaca tggacggcct ggagctgctg cgccagctgc gcgccgaccc tgccacgcgc
     4561 gagctgcgct gcgtcgcgct gtcggccgac gccctgcccg agcagattgc gcgcgcccgc
     4621 gagagcggct tcgacaacta ctggaccaag cccttcgacg tcacccgcct gctgggcgaa
     4681 ctcgaccgtc tactggcctg agagtgcacg caagcggcaa agaaaaaggc ccggagaaac
     4741 cgggcctttg agaattgctc gcaaatcaga cgacagtcac ttgaggtggc tgttgaccag
     4801 cttggtcatc tcgaacatcg aagcctgggc cttcttgaag atctccttca gcttggcgtc
     4861 ggcattgatc atgcgcttgt tgaccgagtc ctggagcttg ttcttcttga tgtagtccca
     4921 gatcttcttg gtcacttcgg tgcgcggcag cggcttgtca cccacgatgg cggccagcgc
     4981 ggcgctgggc gtcagggcct tcatgaaggc cgcattgggg gtgcgcttct tggcgggcgc
     5041 ggccttcttg gcaggagcct tcttcgccgc agccttcttg gccggggcct tcttcgccgc
     5101 ggccttcttc gccggagccg ccttcttggc cgccggcttc ttggccgggg ccttcttcgc
     5161 cgccgctttc ttcgcaggcg ctttcttcgc agttgccatg ttgaatatct ccatcaaagt
     5221 tggttgatgt gccgggcccc gggcgaatag ccgcatgagc tttcgttctc tcagcaaccc
     5281 tgcggggcga atggtactgc gtgagtgctg cattgagaag cagttttcac tcggaaaaca
     5341 cgcattcatg cgggtttgcg cgctgttttg tcgtggcgcg gcatcatttg cgtgacttgc
     5401 ggcgcatcgc gcgcatcgca tcgggcttgc gcaagcgcat cgagagcgct caacgcgctc
     5461 gcaaaaaacg cgctgaaccg ctatcctgcg gcgccatgaa gacgctgctc cgttggctgc
     5521 tgctggcagc cgccctgctg ctggtagccc atctctaccc cggtgtggag gtgaagagct
     5581 tcggctcggc gatgatcgcg gcggccgtac tgggcctgtt caatgccgtg ttgcggccca
     5641 tcctggtcgt gctgacgctg ccggtgacgc tggtgacgct ggggctcttc ctcttcgtca
     5701 tcaacgcgct gatgttttac ggcgcggcct acgtgctcaa gggcttctcg gtgcatggct
     5761 tcgtcgccgc gctgatcggc tcgctgctct acagcgcctg cggtgtcgtg atcgatgcgg
     5821 cgctggagcg gctgttcgtc aagggtgact gagaggctcg tggcctgagc gagcgccgca
     5881 ggcgctgcgc gggcaagcct agctcttcgg ctcttcgctc ttgtccttgt tgttgccgcg
     5941 ctcgtcggcc atttcgcggc gccgcagcgc ctgcacgtcg cgcatgcgcg cctcgatcac
     6001 cgaagcctcg atcgagtcgc gcgccgcgct cttgcgcgcc agggtctggc cggcgtgcag
     6061 gcggtccacg gcgccgtcgt agtcgccgat cgcgacgcgc acctcggcct gggcgcgcac
     6121 ggcccgcagc ggctcgtcca ggcgctccca ggcctgggcg gcgtaggtcc aggccagcgc
     6181 gtccttggga tgcagcgcga cccaggtctg cagttcgtcg acgctgcgcc gcagttgggc
     6241 gcgcggcgtg ccgggcagca aggccagccg cgcctgcagc atcgccaccg ggcgcgtgcc
     6301 gtcgccggca tggggcgcga gcgcggcagc ggcgcgcgcc gcgtcgccgc gctcgatcga
     6361 ggactcggcc tgcagcaagg ccaccgcgcg cgcggcgccg gcatcgccct gcgccagctc
     6421 gcgcgcctgg gcgagcatct cgtcagcgcg cggccattcc ttcagcttgg tggctgccag
     6481 cgcggcggca tagacggagg tcaggcgctc ggccttgctg gttgcggcac cgccctcgtt
     6541 ggccgccacc cgggccagcg cagcagcacg cggatccatc agcgcccggg ctcggccgcg
     6601 ataggccgca tgctccagca ggttcttgct cattgccggg ttctcatcgg cattcacgcc
     6661 cagccgcgca cgcgcctcgc cgatgcgctc ggtggtgagc gggtggctgc gcagataggg
     6721 aaagtctcca ctgtcggtga gccgcgaggc cgcggcgagt cgctcgaaca tgccggccat
     6781 gcccgcgggc tggtagcctg cgccctgcaa cacgccaaag ccgacccggt cggcctcgcg
     6841 ctccctgtcg cgcgagaaat tgagctggcc ctgcgccgtg acggcctggc cgccgacgat
     6901 cagcgcctcc ccagcctgcg ggctgcgcgc agcggcgagg atgccgagga tcatcgacgc
     6961 gatgccgacc atggacacgc gctgctcgtt gccgatcatg cgcgcgatgt ggcgctgggt
     7021 gacgtgcgac atctcatgcg ccagcacaga ggcgagctca tcgctggtgg cggtcatcga
     7081 gatcaggccc agatgcacgc ccacgtagcc gcccggcagc gcgaaggcat tgatggtctt
     7141 gtcggtgacc aggaagggct gccaggcgaa atggtcggac agctcctggg tcaggttgcc
     7201 gcgcgcgcgg gaggcggcca ccaacggc
//
LOCUS       DASUCY010000132         2066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1239849, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000132 DASUCY010000000
VERSION     DASUCY010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1239849"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..1304)
                     /gene="nhaA"
                     /locus_tag="VFV25_02645"
     CDS             complement(93..1304)
                     /gene="nhaA"
                     /locus_tag="VFV25_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEY1392243.1"
                     /translation="MSKAYAAVGAAAGAPQPPAFARFCASETAGGIVLAIAALVALVI
                     SNSPLSAWYQSFLHLPGELRIGSGWLVLNKPLLIWVNDLWMAVFFLLVGLEIKREFVE
                     GELASRRQAVLPAVAALGGMAAPAAIYSLVNWGDAIALRGWAIPAATDIAFAVGIVTL
                     LGSRVPASLKVFLTAVAIIDDLGAIVVIACFYTSQLSLAMLLAAGVGCLVLLALNRLG
                     VTRLSPYIVVGAVIWLCVLKSGIHATLAGVVTAMAIPMRDATGRSPLEAAEQGLHPWV
                     AFVVLPVFAFANAGVSLQGVSLATLFSPIPLGIAVGLVAGKTLGVFGSSWLMVRAGWA
                     GRPQGSSWAQFFGVCVLCGIGFTMSLFIGALAFEGLDAGYETRVKLGVICGSLLAGLL
                     GSLILLRSRRS"
     gene            1402..>2066
                     /locus_tag="VFV25_02650"
     CDS             1402..>2066
                     /locus_tag="VFV25_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEY1392244.1"
                     /translation="MTTIRYDDLVESVAAALQYISYYHPADYISHLARAYAREESPAA
                     KDAIAQILTNSRMSAEGRRPMCQDTGIVNVFMKIGMDVRWEGFSGSILDAVNEGVRRG
                     YNDPDNRLRASIVADPQFDRKNTKDNTPAVVHMEVVPGNTVDVQVAAKGGGSENKSKF
                     VMLNPSDSLVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAVLMAKESLMDDLDMYEL
                     QAKS"
BASE COUNT          417 a          719 c          671 g          259 t
ORIGIN      
        1 gccaaggcct cgggcgcgcc gcccacgccc agccaaagct cgctgagcac atccttggga
       61 gccagagcat ccatggtttt ccttcgctgg cgtcaggaac gccggctgcg caggagtatg
      121 agcgagccca acagccccgc cagcagcgag ccgcaaatga ccccgagctt gacccgggtc
      181 tcatagcccg cgtccaaccc ctcgaaggcc agtgcgccga tgaacaagct catcgtgaag
      241 ccgatgccgc aaagcacgca gacgccgaag aactgggccc agctcgaccc ctgcggccgg
      301 ccggcccagc cggcgcgaac catcagccaa gacgagccga acacgcccag cgtcttgccc
      361 gccaccagcc cgacggcgat gcccagcggt atcggcgaga acagcgtcgc gagcgacacg
      421 ccctgcaacg agacgccggc attggcgaag gcgaacaccg gcagcacgac gaaggcgacc
      481 caagggtgca ggccctgctc tgcggcctcc agcggcgaac gcccggtagc gtcgcgcatg
      541 gggatcgcca tcgccgtgac gacgccagcc agcgtcgcgt ggatgccgga tttcaggacg
      601 cacagccaga tcacggcacc aacgacgata tatgggctga gcctggtgac gccgagccgg
      661 ttcagggcca gcaggacgag acaaccgacg cccgcggcga gcagcatcgc gagcgacagc
      721 tggctggtgt agaagcaggc aatcacgacg atggcgccga ggtcgtcgat gatggccacg
      781 gccgtgagaa agaccttcag cgaggccggc acgcgcgagc ccagcagcgt cacgatgccg
      841 acggcgaagg cgatgtcggt ggccgcgggg atcgcccagc cgcgcagcgc aatcgcgtcg
      901 ccccagttga ccaggctgta gatcgccgcc ggtgcggcca tgccgcccag cgcggcgacg
      961 gccggcagca cggcctggcg gcggctcgcc agctcgcctt cgacgaactc tcgcttgatc
     1021 tccagcccga ccagcaagaa gaacaccgcc atccacagat cgttgaccca gatcagcagc
     1081 ggcttgttga gcacgagcca gccactgccg atgcgcagct cgcccggcag gtgcaggaag
     1141 gactgatacc aggcgctcag cggcgagttg ctgatgacga gggcaaccag ggccgcgatc
     1201 gccagcacga tgccgccggc cgtttcgctg gcgcagaaac gggcgaaggc cggtggttgg
     1261 ggcgcaccgg ccgcggcacc gacggcggca taggccttgg acattgttgt gatcccaggg
     1321 aaagtacact cgaattcttg cctgaatcgc gcacgccgcg ttcaggcccg ccccgttttc
     1381 gacacctccc gagaccccgc catgaccacg atccgctacg acgacctggt ggaaagcgtt
     1441 gccgccgccc tgcagtacat cagctactac caccccgccg actacatcag ccacctggcc
     1501 cgcgcctatg cgcgcgagga gagcccggcc gcgaaggacg ccatcgcgca gatcctcacc
     1561 aattcgcgca tgagcgccga gggccgccgg cccatgtgcc aggacaccgg catcgtcaac
     1621 gtcttcatga agatcggcat ggacgtgcgc tgggaaggct tctcgggctc gatcctcgat
     1681 gccgtcaacg aaggcgtgcg ccgcggctac aacgacccgg ataacaggct gcgcgcttcc
     1741 atcgtggccg acccgcagtt cgaccgcaag aacaccaagg acaacacgcc ggccgtcgtg
     1801 cacatggagg tcgtgcccgg caacacggtg gacgtgcagg tcgcggccaa gggcggcggc
     1861 agcgagaaca agagcaagtt cgtgatgctc aaccccagcg actcgctggt cgactgggtg
     1921 ctcaagaccg tgcccaccat gggcgccggc tggtgcccgc ccggcatgct gggcataggc
     1981 attggcggca ccgccgaaaa agccgtgctg atggccaagg aaagcctgat ggatgatctg
     2041 gacatgtacg agctgcaggc caagtc
//
LOCUS       DASUCY010000133         3917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_99294, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000133 DASUCY010000000
VERSION     DASUCY010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99294"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..1861
                     /locus_tag="VFV25_02655"
     CDS             38..1861
                     /locus_tag="VFV25_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY1392245.1"
                     /translation="MNLRSKSPGRATLSALALSLVAAAVSTGCGSSDSSSSGTSAAAA
                     ATPIQHVVVIFQENVSFDHYFATYPTAANPAGQPAFVAAAGTPAVNTLATPLDVSKGF
                     VPLTGVNLLTNNPTLANAKNGANAINPFRLDRTQAATADQNHAYDAEQQAFDNNAMDL
                     FPATVGIGDAAAPQSLTPPLNTAGLVMGYYDGNTVTALWNYAQNFAMSDNSYGTTFGP
                     STVGALNLISGQTNGVITTNAVAKVTGSNIDGELSADGNGGISVTGDPQPLGDACSTR
                     DQVTLNGQNIGDLLNAANVSWGFFEGGFDLGITNPNGTTGCKRSTTSAVTGVKKVDYI
                     PHHQPFQYYKSTQNLTHARPSAVSAIGNTYQADGKTLDPANHQYDLHDFFDAVSAGNF
                     PAVSFLKAPGYQDGHAGYSDPLDEQAFVVQVINFLQQQPGWANTLVVIAYDDSDGWYD
                     HQASPIVNASALTAATAGFTSNGVAVAGLGDFLNGAGTCKTGLQQQGMSAAPSAALAG
                     ADGKANAQGRCGYGPRMPLMVISPFAKANYVDHTLTDQSSILRFIEDNWLQGKRITGS
                     FDALAGSLNSMLNLSASTGTTAKLFLDPLSGQPTACANCLR"
     gene            2286..3047
                     /locus_tag="VFV25_02660"
     CDS             2286..3047
                     /locus_tag="VFV25_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEY1392246.1"
                     /translation="MLDLTLVTLASLFAGFVDAIVGGGGLILVPALFSAYPQTAPATL
                     FGTNKGAAIWGTGWATAQFARRVQMNWQALLPAALAALLGSFAGAWVVTKVPPTGLRQ
                     ALPLILVGVLAYTLMRKDLGRAHKPRYAGRAEAALAAGIGLVIGFYDGFFGPGTGSFF
                     VFLFVRVLGYDFLHASASAKLLNVATNAAALVLFASTGHIWWQTAFVMALANVAGSLL
                     GTKLALKHGAGFVRGVFIAVVSALILKTAYDAFFR"
     gene            complement(3073..3660)
                     /locus_tag="VFV25_02665"
     CDS             complement(3073..3660)
                     /locus_tag="VFV25_02665"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEY1392247.1"
                     /translation="MKKTVYSLAFAAAALVAAPAAQAQYAPAPVRLVIGGGITGGGDR
                     LATVYYNTGNSYDITAGSLIHVFVGGEWRVAPRVSMQANVGYHFDRTDASNGSIRFER
                     FPIELLAHYDINPVFRLGGGVRFVTGAKLAGTGAVGGDIDFQSTTGLVLEGEYMMSSN
                     LGLKLRAVPVEDYTPSGGGSKLNGSHVGFYLTGYF"
BASE COUNT          655 a         1356 c         1246 g          660 t
ORIGIN      
        1 cccggccatc cctaccagag aagcaaagga tctcacgatg aacctgcgtt ccaaatcgcc
       61 ggggcgtgcc acgctgtcgg ccctggccct gagcctcgtt gcggcggcgg tgtccaccgg
      121 ttgcggcagc tcggacagct cctcctccgg cacgtctgct gcggcagccg ccacgcccat
      181 ccagcacgtg gtcgtgatct tccaggagaa cgtctccttt gatcactact tcgccaccta
      241 tcccacggcg gccaacccgg ccggccagcc tgccttcgtg gcggccgccg gcacgccggc
      301 cgtcaacacc ctcgctacgc cgctggacgt gtccaagggc ttcgtgccgc tgacgggcgt
      361 gaacctgctg accaacaacc cgaccctggc caatgccaag aacggcgcca acgcgatcaa
      421 ccccttccgc ctggaccgca cccaggccgc gacggccgac cagaaccacg cctacgacgc
      481 cgagcagcag gccttcgaca acaacgcgat ggacctgttc ccggccacgg tgggtatcgg
      541 tgacgcggca gcgccgcaat cgctgacgcc gccgctgaac acggccggcc tggtgatggg
      601 ctattacgac ggcaacaccg tgacggcgct gtggaactac gcccagaact tcgcgatgag
      661 cgacaactcc tacggcacca ccttcggccc ctcgacggtg ggcgcgctca acctgatctc
      721 cggccagacc aatggcgtga tcacgaccaa tgccgtggcc aaggtcaccg gctccaacat
      781 cgacggcgag ctctcggccg acggcaacgg cggcatctcc gtgaccggcg atccgcagcc
      841 gctgggcgat gcctgctcga cgcgcgacca ggtgacgctg aacggccaga acatcggcga
      901 tctgctgaac gcggccaatg tcagctgggg cttcttcgag ggcggcttcg acctgggcat
      961 caccaacccc aacggcacca ccggctgcaa gcgctcgacg acctcggccg tcaccggcgt
     1021 caagaaggtg gactacatcc cgcaccacca gcccttccag tactacaagt cgacgcagaa
     1081 cctgacgcac gcgcgcccaa gcgccgtctc ggcgatcggc aacacctacc aggccgatgg
     1141 caagacgctg gacccggcca atcaccagta cgacctgcac gacttcttcg atgccgtgtc
     1201 ggccggcaat ttcccggcgg tgagcttcct caaggcgccg ggctaccagg acggccatgc
     1261 gggctactcc gatccgctgg acgagcaggc cttcgtcgtg caggtgatca acttcctgca
     1321 gcagcagccg ggctgggcca acaccctggt cgtgatcgcc tacgacgatt cggacggctg
     1381 gtacgaccac caggcgagcc cgatcgtgaa cgcctcggcg ctcaccgccg cgaccgccgg
     1441 cttcaccagc aacggcgtgg ccgtcgcggg cctgggcgac ttcctgaacg gcgcgggcac
     1501 ttgcaagacg ggcctgcagc agcaaggcat gtccgctgcg ccgagcgctg cgctcgccgg
     1561 cgccgacggc aaggccaatg cgcaaggccg ctgcggctac ggcccgcgca tgccgctgat
     1621 ggtgatctcg cccttcgcga aggcgaacta tgtcgatcac acgctgaccg accagagctc
     1681 gatcctgcgc ttcattgagg acaactggct ccagggcaag cgcatcaccg gctccttcga
     1741 tgcgctcgcc ggcagcctga acagcatgct gaacctgagc gcctccaccg gcacgacggc
     1801 caagctcttc ctcgatccgc tgtcgggcca gccgacggct tgcgccaact gcctgcgctg
     1861 aggccgactc aggcgaagag cgggcgggta tgttcccgcc ctctcttcac tgcactacgg
     1921 cgctactgtt tcgatatcgc tctttcgtaa attatcttgg gtgactgatg aaaaattctg
     1981 caacagcagg gtgtgtgacg caccgagacc gggccctggc ctccaaataa ggggcaggaa
     2041 aaccccattg tggggcggct gcggcccggt ctacattcct tgtctcgctg caggccttgg
     2101 gcctcgcgcc gcgggggctg aggagacaag atcagcgcaa gagaactaac gagacataag
     2161 gcgagcagcg tcgtgacccg atagatgtcg ccacataatc caaagagcgc cggctaccac
     2221 cggcgctctt tgtttttgtg cttcccgtcg agggcaggcc ccacaagaag aagcgactcg
     2281 tctccatgct cgatctcacc ctcgtcacgc tagcctcgct attcgccggt ttcgtcgatg
     2341 ccatcgtcgg cggcggcggg ctgatcttgg tgccggcgct gtttagcgcc tatccgcaga
     2401 ccgcgccggc cacgctgttc ggcaccaaca agggcgcggc gatctggggc acgggatggg
     2461 ccacggcgca gttcgcgcgc cgcgtgcaga tgaactggca ggcgctgctg ccggccgcgc
     2521 tggcggcatt gctgggcagc ttcgccggcg cctgggtggt cacgaaagtg ccgcccaccg
     2581 ggctgcgcca ggccctgcct ttgatcctgg tgggcgtgct ggcctatacg ctgatgcgca
     2641 aggatctggg ccgcgcgcac aagccgcgct acgccggccg cgccgaagcg gcgctcgcgg
     2701 ccggcatcgg cctcgtcatc ggcttctacg acggcttctt cgggccgggc accgggagtt
     2761 tcttcgtctt tttgttcgtg cgcgtgctgg gctacgactt cctgcacgcc tcggccagcg
     2821 ccaagctgct caacgtggcg accaatgcgg ctgcgctggt gctgttcgcc tccaccggcc
     2881 acatctggtg gcagaccgcc ttcgtgatgg cgctggccaa tgtcgccggc agcctgctgg
     2941 gcacgaagct ggcgctcaag catggcgccg gcttcgtgcg aggcgtcttc atcgccgtgg
     3001 tgtcggcgct gatcctgaag accgcctatg acgctttctt ccgctgaagc cccgaaggcg
     3061 cttcgggggt ggtcagaaat agccggtcag gtagaagccg acatggctgc cgttgagctt
     3121 gctgccgccg ccactgggcg tgtagtcctc caccggcacg gcgcgcagct tcaggccgag
     3181 gttgctcgac atcatgtact cgccttccag caccaggccg gtggtcgact ggaagtcgat
     3241 gtcgccgccc acggcgccgg tgcctgcgag cttggcgccg gtgacgaagc gcacgccgcc
     3301 gcccaggcgg aagacgggat tgatgtcgta gtgggccagc agctcgatcg ggaagcgctc
     3361 gaaccggatg ctgccgttgc tcgcgtcggt gcgatcgaag tggtagccga cattggcctg
     3421 catcgacacg cgcggcgcga cgcgccattc gccgccgacg aagacatgga tcaagctgcc
     3481 ggccgtaatg tcgtagctgt tgccggtgtt gtagtagacg gtggccaggc ggtcgccgcc
     3541 cccggtgatg ccgccgccga tcaccaagcg caccggtgct ggcgcatatt gggcctgagc
     3601 agcgggagcc gcgaccaggg cggccgcagc gaaagcgagg ctgtagacgg ttttcttcat
     3661 ggggaactct gaggtggttg ggtagtccat gcaggctctt gagcccgccc actgtattgg
     3721 cagtgcgtcc aatgactgta agcgccgtgg gtgccggctt cggcaggcct gttgtgctgc
     3781 ggagacaagc tgcaacgcca ggctgccgca tcgcgcgtgc ggacacgctt cgtgacgctt
     3841 tttccgccgg attgccgcgc ctcgccggct ccgccagaat cgcgccgcca tgagaccagc
     3901 ctttgcactt tccttgc
//
LOCUS       DASUCY010000134         2193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_533076, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000134 DASUCY010000000
VERSION     DASUCY010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533076"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..1019)
                     /locus_tag="VFV25_02670"
     CDS             complement(60..1019)
                     /locus_tag="VFV25_02670"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEY1392248.1"
                     /translation="MAVFTEVGFDEAAALMRRLNLGALTEFRGITSGIENTNYFATTE
                     RGDYVLTLFERLTHEQLPFYLHLMRHLAQHGIPVPEPQADASDEILHSLRGKPAAVVN
                     KLLGGHQLAPADSHCESVGAMLARMHLAGLDYPRQQPNLRGLAWWIETVPVVLPFLSA
                     EQRELIEGELAFQQALAGTAAYQALPRGPIHADLFRDNVMFVGDELTGFFDFYFAGVD
                     TLLFDICVCLNDWCLDLDSGRLDEQRAAAFVAAYDGVRQLSGDELRLLAALLRAAALR
                     FWLSRLWDLHLPRDAAMLKAHDPSHFERVLRARVASPWHYTRP"
     gene            complement(1023..1358)
                     /locus_tag="VFV25_02675"
     CDS             complement(1023..1358)
                     /locus_tag="VFV25_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392249.1"
                     /translation="MIPHRLAAPVLAAVLLCAVGLARAQEAAAAKEPPPGPPEPAVRR
                     SVIEDEGSRIEELKVRGQVKSITVTPKVGTKIPYEIVPLDASRDVTPIGPGNTRGIAG
                     QRVWHVLSF"
     gene            1442..>2193
                     /locus_tag="VFV25_02680"
     CDS             1442..>2193
                     /locus_tag="VFV25_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEY1392250.1"
                     /translation="MNSTAPLEPTLLLVDGSSYLYRAYHALPDLRSSQGEPTGAMHGM
                     VNMLRLLRKQYPVAHAACVFDAKGPTFRDEWYADYKANRSPMPEALVAQIAPIHEVVK
                     LLGWPVLEVPGVEADDVIGTLARIAAHSGHKVIISTGDKDLAQLVDEHVTLINTMSNE
                     TLDVAGVKAKFGVPPERIIDYLTLIGDAVDNVPGVEKVGPKTAAKWIEEYGSLDGVMA
                     AADSIKGVAGENLRKALDWLPQGRRLITVKTD"
BASE COUNT          369 a          716 c          776 g          332 t
ORIGIN      
        1 gcgaaagcca tcgcgcgccc acatcaggcc ctcgcggaat ccggcttgct tgagtttcat
       61 caggggcgcg tgtaatgcca ggggctggcc acgcgcgcgc gcagcacgcg ctcgaaatgg
      121 ctggggtcgt gggctttgag catggcggcg tcgcgcggca ggtgcaggtc ccacagccgc
      181 gacagccaga agcgcagggc cgccgcgcgc agcagggcgg ccagcaggcg cagctcgtcg
      241 ccgctcagtt ggcgcacgcc gtcgtaggcg gccacgaagg cggcggcgcg ctgctcgtcg
      301 aggcggccgc tgtcgaggtc taggcaccag tcgttgaggc acacgcagat gtcgaacagc
      361 agcgtgtcga cgccggcgaa gtagaagtcg aagaagccgg tcagctcgtc gccgacgaac
      421 atcacgttgt cgcgaaaaag gtcggcatgt atggggccgc gtggcagggc ctggtaggcc
      481 gcggtgccgg ccagggcctg ctggaaggcg agttcgccct cgatcagctc gcgctgctcg
      541 gcgctcagga aaggcagcac caccggcacc gtctcgatcc accacgcgag gccgcgcagg
      601 ttgggctgct ggcgcggata gtcgaggccc gccagatgca tgcgcgccag catcgcaccc
      661 acgctctcgc aatggctgtc ggccggcgcc agctgatggc cgccgagcag cttgttgacg
      721 acggcggccg gcttgccgcg caggctgtgc aggatctcgt cgctcgcatc ggcctggggc
      781 tcgggcaccg ggatgccgtg ctgcgcgaga tggcgcatca gatgcagata gaagggcagc
      841 tgctcatgcg tcaggcgctc gaacagcgtc agcacgtagt cgccgcgctc ggtcgtcgcg
      901 aagtagttgg tgttctcgat gcccgaggtg atgcctctga actcggtcag cgcgccgaga
      961 ttcaggcgcc gcatcaaggc ggccgcttcg tcgaagccga cttcggtgaa gacggccatg
     1021 gatcagaagc tgagcacgtg ccagacccgc tgccccgcga tgccgcgggt gttgcccggg
     1081 ccgatcgggg tcacgtcgcg cgaggcgtcc agcggcacga tctcataagg tatcttggtg
     1141 ccgaccttgg gggtcacggt gatgctcttg acttggccgc gcaccttcag ctcctcgatg
     1201 cgcgagcctt cgtcctcgat cacgctgcgg cgcaccgccg gctcgggcgg accggggggc
     1261 ggttccttgg cggccgcggc ttcctgcgcg cgcgccagcc cgaccgcgca cagcaacaca
     1321 gcggcaagca caggggcggc aagacgatgc gggatcatgg ttcgattgta ggcggcgcgc
     1381 gagttccgcc ctgtgcgggg acggcgggca ttgacgatcg gcatcaacga gaatcgtccg
     1441 catgaacagc accgcgcctc ttgaaccgac cctgttgctg gtcgatggtt ccagctatct
     1501 ctatcgcgcc taccatgccc tgcccgatct gcgcagctcg cagggcgagc ccaccggtgc
     1561 gatgcacggc atggtcaaca tgctgcgcct gttgcgcaag caatatccgg tcgcgcacgc
     1621 ggcctgcgtt ttcgatgcca agggaccgac cttccgcgac gagtggtacg ccgactacaa
     1681 ggccaaccgc tcgcccatgc ccgaggcgct ggtggcccag atcgcgccca tccatgaggt
     1741 cgtgaagctg ctcggctggc cggtgctgga ggtgccgggc gtcgaggccg acgacgtgat
     1801 cggcacgctg gcgcgcatcg cagcgcacag cggccacaag gtcatcatct cgacaggcga
     1861 caaagatctg gcccagctcg tggacgagca cgtgaccctc atcaacacga tgagcaacga
     1921 gacgctggat gtggccggcg tgaaggccaa gttcggcgtg ccgcccgagc gcatcatcga
     1981 ctacctcacg ctcatcggcg acgcggtgga caacgtgccg ggcgtcgaga aggtcggccc
     2041 gaagacggcg gccaagtgga tcgaggaata cggctcgctg gacggcgtga tggccgcggc
     2101 cgacagcatc aagggcgtgg ccggcgagaa cctgcgcaag gcgctggact ggctgcccca
     2161 gggccggcgg ctcatcaccg tcaagaccga ttg
//
LOCUS       DASUCY010000135         2195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_806147, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000135 DASUCY010000000
VERSION     DASUCY010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806147"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VFV25_02685"
     CDS             complement(<1..657)
                     /locus_tag="VFV25_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY1392251.1"
                     /translation="MNQGQVASAPLLEARELSHHYSVSRGLFKPHATVKALNGVSFSL
                     DAGQTLAVVGESGCGKSTLARALTLIEKPTSGTLNIAGVDAASASSAQQRALRRQVQM
                     VFQNPYASLNPRKRVAQMLEEPLLLNTPLGAAERAERVAEMLNQVGLRPEHARRYPHM
                     FSGGQRQRIAIARAMMLQPGLLVADEPTSALDVSIQAQILNLFMDLQERSGIAYVFIS
                     H"
     gene            complement(654..1643)
                     /locus_tag="VFV25_02690"
     CDS             complement(654..1643)
                     /locus_tag="VFV25_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEY1392252.1"
                     /translation="MSLLEIRNLTVEFGPKERPFAAVQGVDLTLDRGELLGVVGESGS
                     GKSVSMLALMGLIDAPGRVSADVLDFDGHDLLKLAPKERRRIVGRDMAMIFQDPMTSL
                     NPSYTVGYQILETLKVHQSGSKSRLRERAVELLKLVEIPDPESRLAAFPHQLSGGMSQ
                     RVMIAMALACNPKLLIADEPTTALDVTIQAQVLDLLVQLQRKQDMALILITHDLALLA
                     EHARRIAVMYAGEVVESQGVPALFERPHHPYTEALLASIPEHSRGARRLPTLAGVVPG
                     QYDRPAGCLLSPRCPHVQDACRATHPGLSPMPDGGQARCLFPLNVAPAAEATA"
     gene            complement(1640..>2195)
                     /locus_tag="VFV25_02695"
     CDS             complement(1640..>2195)
                     /locus_tag="VFV25_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY1392253.1"
                     /translation="LGLLAAFFPSKLGTLILRLMDVLLALPSLLLAIAVVAVLGPGLL
                     NTMCAIAIVAMPGYVRLVRASAMAELTKDYVVASRLAGAGTLRLMFDTVLPNCMAPVI
                     VTATLCFSDAILTAAALGFLGLGAQPPTPEWGAMLASARDYIEHANWVVTLPGVAILS
                     TVLAINLMGDGLRDALDPRMKKLS"
BASE COUNT          363 a          722 c          781 g          329 t
ORIGIN      
        1 gtgcgagatg aagacatagg cgatgccgct gcgctcctgc agatccatga agaggttgag
       61 gatctgggcc tggatcgaca cgtccagcgc cgaggtcggc tcgtcggcca ccagcaggcc
      121 gggctgcagc atcatcgcgc gggcgatcgc gatgcgctgg cgctggccgc cggaaaacat
      181 gtgcggatag cgccgcgcat gctctgggcg caggcccacc tggttcagca tctctgcgac
      241 gcgctcggca cgctcggccg cgcccagggg cgtgttcagc agcagcggct cttcgagcat
      301 ctgcgccacg cgcttgcgcg ggttcagcga ggcatagggg ttctggaaca ccatctgcac
      361 ctggcggcgc agcgcgcgct gctgcgcaga actggccgag gcggcatcga cgcctgcgat
      421 gttcagcgtg cccgaagtgg gcttttcgat cagcgtcagc gctcgtgcca gcgtggactt
      481 gccgcagccc gattcgccga ccacggccag cgtctggccg gcgtcgagcg aaaagctcac
      541 gccgttgagc gccttcacgg tcgcatgcgg cttgaagagg ccgcgcgaca cgctgtagtg
      601 gtgcgagagc tcgcgcgctt cgagaagcgg ggcggaggca acctggcctt gattcatgcc
      661 gtggcctccg cggcaggcgc gacgttgagc ggaaacaggc agcgcgcctg accgccgtcc
      721 ggcatgggcg aaaggcccgg gtgcgtggcg cggcaggcgt cctgcacatg tgggcagcgc
      781 ggtgacagca ggcagccggc cggccggtcg tactgccccg gcaccacgcc ggccagcgtg
      841 ggcagccgcc gggcaccgcg gctgtgctcg gggatggagg ccagcagcgc ctcggtgtaa
      901 gggtggtgcg gccgctcgaa gagggccggc acgccttggc tctcgaccac ctcgccggca
      961 tacatcaccg cgatgcgcct ggcatgctcg gccagcagcg cgaggtcgtg ggtgatgaga
     1021 atgagcgcca tgtcctgctt gcgctgcagc tgcaccagca ggtccagcac ctgggcctgg
     1081 atggtcacgt ccagcgccgt ggtcggctcg tcggcgatca gcagcttggg gttgcaggcc
     1141 agcgccatcg cgatcatcac gcgctggctc atgccgccgg agagctggtg cggaaaggca
     1201 gcaaggcgcg attccgggtc gggaatctcg accagcttga gcagctccac ggcgcgctcg
     1261 cgcaagcgcg acttgctgcc gctctggtgc actttcagcg tctcgaggat ctggtagccc
     1321 acggtgtagc tcgggttcag gctggtcatc gggtcctgga agatcatcgc catgtcgcgg
     1381 ccgacgatgc ggcggcgctc tttgggtgcg agcttcagca ggtcgtggcc atcgaaatcg
     1441 agcacatcgg cgctgactcg cccgggcgcg tcgatcaaac ccattagcgc gagcatgctg
     1501 acgctcttgc ccgagcccga ttcgccgacc acgcccagca gctcgccgcg atccagcgtc
     1561 aggtcgacgc cttgcacggc cgcgaagggg cgttccttcg gaccgaactc gaccgtgagg
     1621 ttgcggattt caagaagact catgacaact ttttcatccg cggatccagc gcatcgcgca
     1681 ggccgtcgcc catcagattg atcgccagca cggtggacag gatggcgaca ccgggcagcg
     1741 tgacgaccca gttggcgtgc tcgatgtagt cgcgggccga ggccagcatt gcgccccatt
     1801 ccggcgtcgg cggctgggcg cccaggccca ggaagcccag ggccgccgcg gtgaggatgg
     1861 cgtccgagaa gcacagcgtc gcggtcacga tcaccggcgc catgcagttg ggcagcacgg
     1921 tgtcgaacat cagccgcaag gtgccggcgc cggccagccg cgaggcgacc acgtagtcct
     1981 tggtcagctc ggccatcgcc gaggcgcgca ccagccgcac atagccgggc atcgcgacga
     2041 tggcgatggc gcacatcgtg ttcaggaggc cagggccgag caccgcaacg acggcgatcg
     2101 ccagcaacag gctgggcagg gccagcagca catccatcag gcgcaggatc agcgtgccca
     2161 gcttgctcgg gaagaaggcg gccagcaggc caagc
//
LOCUS       DASUCY010000136         4745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_725709, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000136 DASUCY010000000
VERSION     DASUCY010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4745
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725709"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VFV25_02700"
     CDS             complement(<1..281)
                     /locus_tag="VFV25_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392254.1"
                     /translation="MNRPPAPPTPARSRSLWLAYAGLCLLCWTLYSVAGTDWQHGSLR
                     VWDAMYEATWHLGPPMLLGALVLPWTRWLQRSSPPWPARVALHAGAALL"
     gene            complement(418..1146)
                     /locus_tag="VFV25_02705"
     CDS             complement(418..1146)
                     /locus_tag="VFV25_02705"
                     /inference="COORDINATES: protein motif:HMM:NF018473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HEY1392255.1"
                     /translation="MKLAIAALSCAFALLPGLSPAAPSCSANSGAMAPLLVELYTSEG
                     CNSCPPADRWLSSIAKSRSDVIALAFHVDYWDSLGWKDRFADAAYTRRQYEIEAGAGS
                     RFAYTPQVLLNGRDYRSWPFLPAPAGAALVNLSLAHEGSSYVARLTRLPAARGGQLLA
                     YWAVTEDSHVSAVKAGENAGATLKHDAVVREYRAPQRLDAADELTLRFEPRVGSEAGH
                     ARHVVLVITDARSGQVVQAAQVDC"
     gene            1398..3770
                     /locus_tag="VFV25_02710"
     CDS             1398..3770
                     /locus_tag="VFV25_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HEY1392256.1"
                     /translation="MEKILLQIAAELKVRPAQVNAAVELLDGGATVPFIARYRKEATD
                     GLDDVQLRELEARLAYLRELEERRAAVLKSIAEQGKLTPELRAAIEAAPTKQELEDLY
                     LPYKPKRRTKAMIAREAGIEPLADKLFADPSLDPMAEATAFINADAGFADAFAVLDGV
                     RDILSERWAEDATLVGKLREWLWGEGLFKSKLMDGKDENHPDIAKFRDYFDYDEPINR
                     VPSHRALAVFRGRALEFLDAKLVLDEEVVPGQPRDPKGPPPVSLAEGHIARHLGWSHG
                     KRASDDLIRKCVAWAWKVKLSLSLERDLFARLREEAEKVAIKVFAENLRDLLLAAPAG
                     KRVVMGLDPGIRTGVKVAVVNETGKVVDTATVYPHEPKRDWEGSIHALGRLAATHGVN
                     LIAIGNGTASRETDKLAADLIKRIQQMAPGTPLEKVVVSEAGASVYSASEYASKELPE
                     LDVSLRGAVSIARRVQDPLAELVKIEPKSIGVGQYQHDVNQSELAKSLGAVVEDCVNS
                     VGVDLNTASAPLLARVSGLSSAVAASIVRWRDANGAFKNRRQLREVSGLGDKTFEQAA
                     GFLRIRDGDNPLDQSGVHPETYPVVEKMLASTGKGIGELMGKSDVIHTLKPEAFADEK
                     FGAITVKDILAELEKPGRDPRPDFKVARFNEGVEDIKDLAEGMVLEGTVSNVAQFGAF
                     IDLGVHQDGLVHVSQLSNKFVNDAREVVKAGDIVKVKVLEVDLVRKRISLTMKLDAAP
                     APRGERGDNSYRPAARNERHGGGGARGSAAAQPAGQSAMAAAFAKLQAKR"
     gene            3820..>4745
                     /locus_tag="VFV25_02715"
     CDS             3820..>4745
                     /locus_tag="VFV25_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HEY1392257.1"
                     /translation="MQALRIYAGPAARAHIENKGLSPQNIRVVAGAAGGPKGLVLGHL
                     DRDIFGRWLRGSGQTIHLVGASIGAWRMATGAMRDPDAAFQRLSSDYIHQEYKVEPGR
                     ETPSAEQVSREFAAELRAFFDGEVEQLIAHPRYRLHIVTSRGRHLLGREGRLRTPLGY
                     LGATLSNAFTRKALSAWLERVVFSSPGEAMPVDLSDLRHRAVRLTAENFRPALLASCS
                     IPFLLKAVHDIPGGPPGAYWDGGITDYHLHWNYRSMSGGGLVLYPHFQQSVVPGWLDK
                     AFKHRHRATPFLDNVIVLAPAPEWVATLPNAKL"
BASE COUNT          832 a         1614 c         1616 g          683 t
ORIGIN      
        1 aagagcagcg cggcgccggc gtgcagggcc acgcgcgccg gccacggcgg gctgctgcgc
       61 tgcagccagc gcgtccaggg cagcacgagg gcgcccagca gcatcggtgg gcccaggtgc
      121 caggtggcct cgtacatcgc atcccacacg cgcaggctgc cgtgctgcca gtcggtgccg
      181 gccacgctgt agagcgtcca gcacagcagg cacaggcccg cataggccag ccagagggag
      241 cggctgcggg cgggagtcgg tggggcagga gggcgattca cgggcgccat cctagtgccc
      301 tatcgggacg gcgcggggat agaggcgatg aaatgcgaaa cgggcgggtg caaacgcggc
      361 tgcggcggcc ccgcaggcct gagatcggcc cgcgcagcag aaagattcac accttcttca
      421 gcaatccacc tgcgccgcct gcaccacctg gccgctgcgc gcgtcggtga tcacgagcac
      481 cacatgccgc gcatggccgg cctccgagcc gacacgcggc tcgaagcgca gcgtcagctc
      541 gtcggccgca tccagccgct gcggcgcgcg gtattcgcgc actacggcgt cgtgcttgag
      601 tgtggctccg gcgttctcgc cggccttgac ggcgctcacg tggctgtcct cggtcacggc
      661 ccaataagct agtagctgcc cgccccgcgc cgcgggcagg cgggtcagcc tggcgacata
      721 gctcgatccc tcgtgcgcca ggctcaggtt gacgagcgcc gcaccggcag gcgccggcag
      781 gaagggccag gagcggtagt cgcgcccgtt gagcagcact tgaggcgtgt aagcgaagcg
      841 cgaacccgcg ccggcctcga tctcgtactg gcgccgcgtg taggccgcgt cagcgaagcg
      901 gtccttccag cccaggctgt cccagtagtc gacatggaag gccagcgcga tcacgtcgct
      961 gcgcgacttg gcgatgctgc tgagccagcg gtcggccggc gggcaggagt tgcagccctc
     1021 cgaggtgtag agctcgacca acagcggcgc catggcgccg ctgttggcgc tgcagcttgg
     1081 cgcggccggg ctcaagccgg gcagcagggc gaaggcgcaa gacaaggcag caatggcgag
     1141 cttcatggtg gggctttctt cacaacaggc atggcatgtg tcggtttagc ccaggtcgac
     1201 ctgacacgga aagagaaatt caaagaaata agctagtcac tcgaacaaat ttaccgaata
     1261 tcatatcaaa aaatgtagcg acacgagccg cggaaccgat cggcaaattc tgctcacaag
     1321 ctcgcgcgcc gtcacggcaa tcgcctccac ccgctgtggc aaactcgcgc gcttcccgtg
     1381 ctcagacgcc ttaggttttg gaaaagatcc tgctgcaaat cgccgccgaa ctgaaggttc
     1441 ggccggccca agtgaatgcc gccgtcgaac tgctggacgg cggggccacc gtccccttca
     1501 tcgcccgcta ccgcaaggag gccaccgacg gcctggacga cgttcagttg cgcgagctcg
     1561 aagcgcgcct ggcttacttg cgcgagctgg aggagcgccg cgccgccgtg ctcaagagca
     1621 tagccgagca aggcaagctc acgcccgagc tgcgcgccgc catcgaggcc gcgccgacca
     1681 agcaggagct cgaagacctc tacctgccct acaagcccaa gcgccgcacc aaggccatga
     1741 tcgcgcgcga agccggcatc gagccgctgg ccgacaagct gttcgccgac ccctcgctcg
     1801 acccgatggc cgaggccact gcattcatca atgcggacgc cggctttgcc gacgcgtttg
     1861 ccgtgctcga cggcgtgcgc gacatcctct ccgagcgctg ggccgaggac gccacgctcg
     1921 tgggcaagct gcgcgaatgg ctgtgggggg agggcttgtt caagtccaag ctgatggacg
     1981 gcaaggacga gaaccacccc gacatcgcca agttccgcga ctacttcgac tacgacgagc
     2041 cgatcaaccg cgtgccctcg caccgcgccc tggccgtgtt ccgcggccgc gcgctggagt
     2101 tcctggacgc caagctggtg ctcgacgagg aggtcgtgcc gggccaaccg agggacccga
     2161 aggggccccc tccggtgtcc ttggccgaag gccacatcgc gcgccacctc ggctggagcc
     2221 acggcaagcg cgcaagcgac gacctgatcc gcaaatgcgt ggcctgggcc tggaaggtca
     2281 agctcagcct gagcctggag cgcgatctgt tcgcgcgcct gcgcgaggag gcggagaagg
     2341 tggctatcaa ggtcttcgcc gagaacctgc gcgacctgct gctggcggcg cccgccggca
     2401 agcgcgtcgt gatggggctg gaccccggca tccgcaccgg cgtgaaggtg gccgtggtca
     2461 acgagaccgg caaggtggtg gacaccgcga ccgtctatcc gcacgagccc aagcgcgact
     2521 gggaaggctc catccacgcg ctgggccgcc tggcggccac gcatggcgtg aatctcatcg
     2581 ccatcggcaa cggcaccgcc agccgcgaga ccgacaaact ggcggccgac ctcatcaagc
     2641 ggatacaaca gatggcgccc ggcacgccgc tggagaaggt ggtggtgagc gaagccggcg
     2701 cctcggtgta ctcggcctcc gaatacgcga gcaaggagct gcccgagctc gacgtgagcc
     2761 tgcgcggcgc ggtgtcgatc gcacgccgcg tgcaggaccc gctcgccgaa ctggtgaaga
     2821 tcgagcccaa gagcatcggc gtgggccagt accagcacga cgtgaaccag agcgagctcg
     2881 ccaagagcct gggcgcggtc gtcgaggatt gcgtgaactc ggtcggcgtg gacctcaaca
     2941 ccgcgtcggc gccgctgctc gcgcgcgtct cgggcctgtc atcggcggtg gccgccagca
     3001 tcgtgcgctg gcgcgatgcc aacggcgcct tcaagaaccg tcgccagctg cgcgaagtct
     3061 ctggcctggg cgacaagact ttcgagcagg ccgccggctt cctgcgcatc cgcgacggcg
     3121 acaacccgct ggaccagtcc ggcgtgcacc ccgagaccta tccggtggtc gagaagatgc
     3181 tcgcctccac cggcaagggc atcggcgagc tgatgggcaa gagcgatgtg atccacacgc
     3241 tcaagcccga agccttcgcc gacgagaagt tcggcgccat cacggtgaag gacatcctcg
     3301 ccgagctgga aaagcccggc cgcgacccgc gcccggactt caaggtcgcg cgcttcaacg
     3361 agggcgtcga agacatcaag gacctggccg agggcatggt gctcgaaggc acggtgagca
     3421 acgtcgcaca gttcggcgct tttatcgacc tgggcgtgca tcaggatggc ctggtgcacg
     3481 tgagccagct ctccaacaag ttcgtcaatg atgcacgcga ggtcgtgaag gccggcgaca
     3541 tcgtcaaggt caaggtgctg gaggtggatc tggtgcgcaa gcgcatctcg ctgacgatga
     3601 agcttgatgc ggcgccggca ccgcgcggcg agcggggcga caacagctac cggccggccg
     3661 cacgcaacga gcgccatggc ggtggcggtg cgcggggtag tgctgcggca cagccggcgg
     3721 ggcagagcgc gatggcagcg gccttcgcga agctgcaagc caagcgctga ggctgggggc
     3781 acgcattggg ctcgcaaaga gcccctaaca tcccgcccca tgcaagcact gcgcatctat
     3841 gccggccccg ccgcccgggc ccatatcgag aacaagggcc tgagcccgca aaacatccgc
     3901 gtcgtggccg gcgcggcggg cgggcccaag ggcctggtgc tgggccatct cgaccgcgac
     3961 atcttcggcc gttggctgcg cggttccggg cagaccatcc atcttgtcgg cgcctcgatc
     4021 ggggcctggc gcatggccac cggcgcgatg cgcgaccccg acgctgcctt ccagcggctg
     4081 tctagtgact acatccatca ggaatacaag gtcgagcccg gccgcgagac gccctcggcc
     4141 gagcaggtga gccgcgagtt cgccgctgag ctgcgcgcct tcttcgatgg cgaggtcgaa
     4201 cagctcatcg ctcacccgcg ctatcggctg cacatcgtca cctcacgcgg ccggcatctg
     4261 ctggggcggg aagggcggct gcgcacgccg ctgggctatc tgggcgcgac gctcagcaat
     4321 gccttcacgc gcaaggccct gagcgcctgg ctggagcgcg tcgtgttctc gtcgccgggc
     4381 gaggcgatgc cggtggacct gagcgacttg cgccaccgcg cagtgaggct cacggccgag
     4441 aacttccgtc ccgccttgct ggcctcctgc tcgattccct ttctgctcaa ggccgtgcac
     4501 gacattcccg gcgggccgcc cggtgcctat tgggacggcg gcatcaccga ctaccacctg
     4561 cactggaact accgctcgat gagcggcggc ggcctcgtgc tctatccgca tttccagcag
     4621 agcgtggtgc cgggctggct ggacaaggcc ttcaagcatc gccatcgcgc gacgcctttc
     4681 ctcgacaacg tgatcgtgct ggcgcccgcg cccgaatggg tcgcgacgct gcccaatgcc
     4741 aagct
//
LOCUS       DASUCY010000137         4396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_67282, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000137 DASUCY010000000
VERSION     DASUCY010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67282"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VFV25_02720"
     CDS             complement(<1..555)
                     /locus_tag="VFV25_02720"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120526.1"
                     /note="catalyzes the interconversion of D-xylose to
                     D-xylulose; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HEY1392258.1"
                     /translation="MKNYFHAIPAIEFKGAQSEDPLSFRHYDKNRVVLGKRMEDHLRF
                     AVCYWHTFVWTGLDPFGGQTFERPWFQGGSPMELARAKAGAAFEFFSKLGAPYYCFHD
                     RDVAPEGATPRESHENFLRMVDVLGEHQQRTGVKLLWGTANLFSHRRFMAGAASNPDP
                     AVFAMAALQVRDAMDATLKLGGENY"
     gene            complement(588..2066)
                     /gene="xylB"
                     /locus_tag="VFV25_02725"
     CDS             complement(588..2066)
                     /gene="xylB"
                     /locus_tag="VFV25_02725"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HEY1392259.1"
                     /translation="MYLGIDLGTSEVKLLLLNEAGQILGHAGAPLQVSRPQPLWSEQA
                     PHAWWRATESAVARLRSTHPDEFAQIRAIGLSGQMHGAVLLGARDELLRPAILWNDGR
                     SHAECEELTQRAPMLHAVAGNLAMPGFTAPKLLWVRKHEPDVFAQLRCVLLPKDYLRL
                     MLTGEKVSEPSDAAGTLWLDVAARDWSDALLSACDLRRSQMPRLVESDQPCATLRPEV
                     AKAWGLGPEVVIAGGAGDNAASAIGIGAVQAGDGFISLGTSGVLFVVNDRFRPNPASA
                     VHAFCHALPQRWHQMSVMLSAASCLQWFVGLSGARDEAALLGDVAQLPVESLSNAPIF
                     LPYLAGERTPHNDPFAKGVLFGLTHEHTRAAAAYAVLEGVSFGMADGLAALRAAGTEV
                     TRLSLVGGGARSGWWAQLMADALDVEIDVHASSEAGAALGAARLARLADGGAETQVCT
                     KPEVAAHYLPDPRRHAVLQQRLQRFREIYQRLKPLSFAGGTA"
     gene            complement(2066..3295)
                     /locus_tag="VFV25_02730"
     CDS             complement(2066..3295)
                     /locus_tag="VFV25_02730"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEY1392260.1"
                     /translation="MSVDTAGSQQLLKDINRMALVRQLCATPGLSRADLATAVGLTKS
                     TVSLLVRELMAEGWLAEREVVATGDLGRRPTPLFIDPSRLLLLGAEVGIESVRVVATS
                     LTGDVLARAVTSYGSSKSAKACVGTLASTLLKVRKQLDEATHQIVGIGVGLPGGVDEA
                     RGFLHFAPNLGWRDVPVGTLLTERLADSALAGVPLFLQNEADVAALGELEFSPADVTD
                     PLLYLSINQGVGAGVIVGDRLLTGSRGFAGEVGHIVLQLNGPRCSCGRRGCAEALIGP
                     RAMLQADDKEHPHPLAEIQRRLAEQDDDTVRAVKKAGSYLGVLLQNLASAYDPGRIVL
                     GGAVVDLGDVFLQPALHTLNEYAAAADLAPPAVQTSRFGADAVAVGAAALVRYRLTRP
                     VIGKTPVNHLAADGEAG"
     gene            3589..3810
                     /locus_tag="VFV25_02735"
     CDS             3589..3810
                     /locus_tag="VFV25_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392261.1"
                     /translation="MSAAVTFSSHETGFELCFESLFGHGRGFAFPCDPQGRIDFDAVS
                     DRARNNYLFARAMIGREYAVPIVRRSGSP"
     gene            4019..>4396
                     /locus_tag="VFV25_02740"
     CDS             4019..>4396
                     /locus_tag="VFV25_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HEY1392262.1"
                     /translation="MNVKAIRRIVIVGGGTAGWMTAAPLAQMLGRHAHEVVLIESAEI
                     GAVGVGEATLPPIRAYNQTLGLDGADFIRKTQATFKLGIEFRDWGRLGHRFFHGFGDF
                     GPPIGHRSSFMHWLRLHRAGGMPS"
BASE COUNT          705 a         1452 c         1517 g          722 t
ORIGIN      
        1 gtagttctcg ccgccgagct tcagcgtggc gtccatcgca tcgcgcacct gcagcgccgc
       61 catcgcgaac acggcggggt ccgggttgct tgccgcgccg gccatgaagc ggcggtggct
      121 gaacagattg gccgtgcccc acagcagctt cacgccggtg cgctgctggt gttctcccag
      181 cacgtcgacc atgcggagga agttctcgtg gctctcgcgc ggcgtggcgc cttcgggggc
      241 cacatcgcgg tcatggaagc agtagtaggg cgcgcctagc ttggagaaga actcgaaggc
      301 cgcgccggcc ttggcgcgcg ccagttccat cggcgagccg ccttggaacc aggggcgctc
      361 gaaggtctgt ccgccgaacg gatcgaggcc ggtccagacg aaggtatgcc agtagcacac
      421 cgcgaagcgc aggtgatctt ccatgcgctt gccgagcacg acgcggttct tgtcgtagtg
      481 gcgaaagctc agcgggtcct cgctttgcgc gcccttgaat tcgatcgccg ggatagcgtg
      541 gaaatagttc ttcatgacag ggtgagggtg aattggagtg gttgttctca ggcggtgccg
      601 ccggcgaacg acaggggctt gagccgttga tagatttcgc ggaagcgctg caggcgctgc
      661 tgcagcacgg cgtggcgtcg cgggtcaggt aggtagtgag ccgccacctc gggcttggtg
      721 cacacctgtg tctcggcgcc gccgtcggcg agccgggcca ggcgggccgc gcccagggcc
      781 gcgccggcct cgctgctggc atggacgtcg atctcgacat ccagcgcatc ggccatgagc
      841 tgcgcccacc atccgctgcg cgcgccgccg ccgaccaacg acaggcgcgt cacctcggtg
      901 ccggccgcgc gcaaggccgc caggccatcg gccatgccga agctcacgcc ttccagcacg
      961 gcataggcgg ctgccgcgcg cgtgtgctca tgagtcaggc cgaagagcac gcccttggcg
     1021 aagggatcgt tatgcggtgt ccgctcgccc gcgaggtagg gcaggaagat cggcgcgttg
     1081 gacagcgatt cgacgggtag ttgggccacg tcgcccagca gcgcagcctc gtcgcgcgcg
     1141 ccgctcaagc ccacgaacca ttgcaggcag ctcgcggcgg acagcatcac gctcatctga
     1201 tgccaacgct gcggcagggc atggcagaag gcgtgcacgg ccgaggcagg attggggcga
     1261 aagcggtcgt tcaccacgaa cagcacgccc gaggtgccca gcgagatgaa gccatcgccc
     1321 gcttgcacgg cgccgatgcc gatcgcgctg gccgcgttgt cgccagcgcc gccggcgatg
     1381 accacctccg gccccagccc ccaggccttg gcgacttccg ggcgcaaggt ggcgcaaggc
     1441 tgatcgctct ccaccaggcg cggcatctgg ctgcgacgca agtcgcaggc cgagagcagg
     1501 gcatcggacc agtcgcgcgc ggcgacgtcg agccacaggg ttccggccgc atccgaaggc
     1561 tcggacacct tctcgccggt gagcatcagg cgcaggtagt ccttgggcag cagcacgcag
     1621 cgcagttgcg caaagacgtc gggttcatgc ttcctgaccc acagcagttt cggcgccgtg
     1681 aagccgggca tggcgaggtt gccggccaca gcgtgcagca tcggtgcgcg ctgcgtcagt
     1741 tcctcgcatt ccgcatggct gcggccgtcg ttccaaagta tggcggggcg caggagctcg
     1801 tcgcgcgcgc cgagcagcac ggcgccgtgc atttgcccgg acagtccgat cgcgcgtatt
     1861 tgggcgaact cgtccggatg cgtgctgcgc agacgcgcca cggcgctctc cgtcgcccgc
     1921 caccaggcgt ggggcgcctg ctccgaccac aagggctgcg gccgcgacac ctgcagcggt
     1981 gcgcccgcat gaccgaggat ctggccggct tcattgagaa gcaacagctt gacttcggag
     2041 gttccgaggt cgatgccgag gtacatcagc cggcctcccc gtcggccgcc aaatggttga
     2101 ctggcgtctt gccgatcacc ggccgcgtca agcgatagcg cacgagcgct gccgcgccca
     2161 cggctacggc atccgcgccg aagcgcgagg tctgcactgc gggcggagcc aggtcggccg
     2221 cggccgcgta ttcgttcagg gtgtgcagtg ccggctgcag gaagacgtcg cccaggtcca
     2281 ccacggcgcc gccgagcacg atgcggccgg gatcgtaggc ggaggcgagg ttctgcagca
     2341 gcacacccag gtagctgccg gccttcttca cggcacgtac cgtgtcgtcg tcctgctcgg
     2401 ccaggcgccg ctggatctcg gccagcgggt gcggatgttc cttgtcgtcg gcctgcagca
     2461 tggcgcgcgg gccgatcagc gcttcggcgc agccccgccg accgcacgag cagcgtggcc
     2521 cgttgagctg cagcacgatg tggccgacct cgcccgcgaa gcctcggctg ccagtgagca
     2581 gccggtcgcc gacgatgacg ccagcaccca cgccctggtt gatgctcagg tagagcaggg
     2641 ggtccgtcac gtcggcggga ctgaactcca gttcgcctaa agcggccacg tcggcttcgt
     2701 tctgcaggaa gagcggaacg ccggcgagtg ctgagtccgc gagcctctca gtgagcagcg
     2761 tgcccaccgg cacgtcgcgc cagcccaggt tgggcgcgaa atgcaggaag ccgcgcgcct
     2821 cgtccacgcc gcccggcagg ccgacgccga tgccgacgat ctggtgcgtg gcttcatcga
     2881 gttgtttgcg caccttgagg agcgtgctgg ccagcgtgcc cacgcaggcc ttggcgctct
     2941 tgctcgagcc gtaactggtc accgcgcgtg cgaggacgtc gccggtgagc gaggtcgcga
     3001 ccacgcggac cgactcgatg cccacctcgg cgcccagcag cagcaagcgg ctcgggtcga
     3061 tgaacagcgg tgtggggcgc cggccgagat cgcctgtggc gaccacttcg cgctccgcga
     3121 gccagccctc ggccatcagc tcgcgcacga gcaggctgac ggtggacttg gtcaagccca
     3181 ccgcggtggc aaggtctgcc cgcgacaggc ccggggtggc gcacagctgg cgcaccaagg
     3241 ccatgcggtt gatgtctttg agcagctgct ggctgccggc ggtgtcgacg gacatggtgt
     3301 ctacccccct tccatagata gcggttgcac gatggccggc atggtagttg gggcgtcgat
     3361 cgaatgatta cggtgtcgct atattagttt gggcatcgaa cgaatttaaa cagtgttaac
     3421 acctagctcg atctcgtgcc tgcactcggc gccttcgcgg tcgaccctcg ctgtgctagg
     3481 tatttctccc actttgttcg ggcgttgcac gaactatcat cgatgagcga gagtggcctg
     3541 gattcaaggt gcagcgctac ctgtacggac gaattggcga ggattgccat gtcagcagca
     3601 gttacttttt caagtcatga gaccgggttt gagctgtgct tcgaatctct cttcggccac
     3661 ggaagggggt ttgcctttcc gtgcgatccg caggggcgca tcgatttcga tgccgtcagc
     3721 gaccgtgccc gcaacaacta cctatttgcg cgcgcgatga tcgggcgcga atatgccgta
     3781 ccgatcgtgc gccgaagcgg tagcccttga aggcgatccc aacgctgcgc cggcaaggcg
     3841 acgagcagcg attgctccag ggcataagtt caagccttcg ccgccaagtg cgcggagcat
     3901 gccgtggggc gatctgtaaa cacctagcca catccgtccg gcgcgggcgc agacttcgcg
     3961 ccgcagcgca cggcagtggg gccgtgccgg caactatcca gcggaggtgg aagcaaccat
     4021 gaacgtcaag gccatccgca ggatcgtcat cgtcggcggc ggaacggccg ggtggatgac
     4081 cgccgcgccg ctggcccaga tgctgggcag gcatgcgcac gaggtggtgc tgatcgagtc
     4141 tgccgagatc ggcgccgtgg gcgtgggcga ggcgacgctg ccgcccatcc gcgcctacaa
     4201 ccagaccctg ggcctggacg gcgccgactt catccgaaaa acgcaggcca ccttcaagct
     4261 cggcatcgag ttcagggact ggggccggct ggggcaccgc ttcttccacg gcttcggcga
     4321 cttcggcccg ccgattggcc atcgctcgtc cttcatgcat tggctgcgtc tgcatcgcgc
     4381 cggcggcatg ccgtcc
//
LOCUS       DASUCY010000138         2321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_918552, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000138 DASUCY010000000
VERSION     DASUCY010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918552"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="VFV25_02745"
     CDS             complement(<1..683)
                     /locus_tag="VFV25_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArgP/LysG family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY1392263.1"
                     /translation="MKDLDPAALDCLAALADEGGFERAAQRLAITQSAVSQRLRAMEA
                     QIGQPLVVRSRPLRLTESGKVLLRLARQWHALRADAARELGGSGGGADERLPIAVNAD
                     SLATWVLPALDALVQEGQRAGYGLELVVDDQDFTHDWLRQGAVLGCVSSVSQALRGCR
                     VTPLGVMRYCAVASPAFVDKLLGGDARRGLHEGNFASAPFLVFNRKDDMQAQWVRRAF
                     GVRAPRLNER"
     gene            757..1410
                     /locus_tag="VFV25_02750"
     CDS             757..1410
                     /locus_tag="VFV25_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEY1392264.1"
                     /translation="MPVAASSLLPDAPAFARGWLMSAGLIVAIGAQNALVLRQGLARC
                     HVGPVVALCVASDWLLIAAGVYGLGALIATSPQALELLRWGGAAWLLVYSALAAKRAL
                     ASEHAILSKAAGAASLKATLVATLAMTWLNPHVYLDTVVLLGSVGAQQPGVAARSAFA
                     AGAGFASLMWFLALGYGAAAAAPWLRRPQLWRAIDLAVAGVMFLLAWQLIAGPLLGN"
     gene            1420..>2321
                     /locus_tag="VFV25_02755"
     CDS             1420..>2321
                     /locus_tag="VFV25_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY1392265.1"
                     /translation="MRVLVLGAGIIGVSTAWHLLEEGHEVTVVDRQADAALETSFANG
                     AQISVSFCEPWASAEAPLKVLKWLAHDDSPLLFRPRLDPAQWRWGLSFLTQCTDGAFE
                     RNVEQLVQLGRYSHEALKAVVARTDIEYDRLERGILHFFSSQKDFDAGAEGAEIMRRH
                     GVDRRVLSRDEVLQVEPSLAAYAAKIAGGTYTPSDESGDARIFTQELAKRCVARGARF
                     IYEHDIVTLQATGGELQSVVIADLRTGQKATLSADAYVAALGPYTAPLLRQVGVHLNI
                     YPAKGYSATLKLKRPERASVVSMI"
BASE COUNT          337 a          820 c          794 g          370 t
ORIGIN      
        1 cgctcgttca gccgtggggc gcgcacgccg aaggcgcgcc tgacccattg cgcctgcatg
       61 tcgtccttgc ggttgaacac gagaaaaggg gcgctcgcga aattgccttc atgcaggccg
      121 cgcctggcgt cgccgcccag cagcttgtcg acgaaggcgg ggctcgcgac ggcgcaataa
      181 cgcatcacgc ccagcggcgt cacgcggcag ccgcgcaagg cctggctgac gctggacacg
      241 cagcccagca cggcgccctg gcgcagccag tcgtgggtga agtcctggtc gtccaccacc
      301 agctccaggc cgtagcccgc gcgctgcccc tcctgcacca gcgcgtccag cgccggcagc
      361 acccatgtcg cgaggctgtc ggcgttcacc gcgatcggca ggcgctcgtc ggcaccgccg
      421 ccgctgccgc ccagctcgcg tgccgcgtcg gcgcgcagcg catgccattg gcgggccaag
      481 cgcagcagta ccttgcccga ttcggtcagg cgcaaggggc gcgagcgcac caccagcggc
      541 tggccgatct gcgcttccat cgcgcgcagc cgctgcgaca cggccgactg cgtgatcgcc
      601 agccgctgcg ccgcgcgctc gaagccgcct tcgtcggcca gcgcggccag gcaatcgagc
      661 gcggcaggat ccaggtcctt cataagtcct gctaatgtaa tctgaagaat atgcatgtgg
      721 cttcatgttt tcaacggctc tgccgagaat cccgcaatgc ctgtcgccgc ctcatcgctg
      781 ctgcccgatg ccccggcctt tgcccgcggc tggctgatgt cggccggctt gatcgtcgcg
      841 atcggcgcgc aaaacgcgct ggtgctgcgc caagggctgg cgcgctgcca tgtgggcccg
      901 gtcgtggcgc tgtgcgtggc ctccgactgg ctgctgatcg ccgccggcgt ctacggcctc
      961 ggcgcgttga tcgccacctc gccgcaggcc ctggagctgc tgcgctgggg tggtgcggcc
     1021 tggctgctgg tctatagcgc cctggcggcg aagcgtgcac tcgccagcga gcatgcgatc
     1081 ctgagcaaag ccgccggggc ggccagcctc aaggccacgc tcgtcgcgac actggcgatg
     1141 acctggctga acccgcatgt ctatctggac acagttgtgc tgctcgggag cgtgggtgct
     1201 cagcagcccg gcgtggccgc gcgcagcgcc ttcgcggccg gcgccggctt cgcgtcgctg
     1261 atgtggttcc tggcgctcgg ctatggcgct gccgccgcgg cgccctggct gcgccggccg
     1321 cagctgtggc gcgccatcga cctggccgtg gccggcgtga tgtttctgct ggcctggcag
     1381 ctgatcgccg ggcctttgtt gggcaattag gagagaaaaa tgagagtcct cgtcctcggc
     1441 gccggcatca tcggcgtttc aaccgcctgg catctgctcg aagaaggcca tgaggtcacg
     1501 gtcgtcgacc gccaggccga tgcggcgctg gaaaccagct tcgccaatgg cgcgcagatc
     1561 tcggtcagct tctgcgagcc ctgggccagc gccgaggcgc cgctgaaggt gctgaagtgg
     1621 ctggcgcacg acgattcgcc gctactgttt cgcccccgtc tcgatccggc gcaatggcgc
     1681 tggggcctga gcttcctgac gcaatgcacc gacggcgcct tcgagcgcaa tgtcgagcag
     1741 ctggtgcagc tgggccgcta cagccacgag gcgctgaagg ccgtcgttgc caggaccgac
     1801 atcgaatacg accggctgga gcgcggcatc ctgcatttct tcagctcgca gaaggacttc
     1861 gacgccggcg ccgagggggc ggagatcatg cgccgccacg gcgtggaccg ccgcgtgctg
     1921 agccgcgacg aggtgctcca ggtcgagccc tcgctggccg cctatgccgc caagatcgcc
     1981 ggcggcacct acacgcccag cgacgagtcg ggcgatgccc gcatcttcac gcaggagctg
     2041 gccaagcgct gcgtcgcgcg cggcgcccgt ttcatctacg agcacgacat cgtgacgctg
     2101 caggccaccg gcggcgagct gcaaagcgtg gtgatcgccg acctgcgcac cgggcagaag
     2161 gccacgctga gcgccgatgc ctatgtcgcg gcgctcgggc cttacacggc gccgctgctg
     2221 cggcaagtgg gcgtgcacct caacatctat ccggccaagg gctattcggc cacgctcaag
     2281 ctcaagcggc cggagcgcgc cagcgtcgtc agcatgatcg a
//
LOCUS       DASUCY010000139         2236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_629585, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000139 DASUCY010000000
VERSION     DASUCY010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629585"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..440)
                     /locus_tag="VFV25_02760"
     CDS             complement(99..440)
                     /locus_tag="VFV25_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEY1392266.1"
                     /translation="MDRVILPTLETPTLTKAELADLLFERLGLNKRESKDMVEAFFEL
                     VHGTLVQGKDVKLSGFGNFQIRRKAPRPGRNPRTGEAIPIKARNVVTFHASHKLKGVV
                     QGDISAEENFE"
     gene            complement(483..>2236)
                     /gene="pheT"
                     /locus_tag="VFV25_02765"
     CDS             complement(483..>2236)
                     /gene="pheT"
                     /locus_tag="VFV25_02765"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEY1392267.1"
                     /translation="GRVIKGVNPKASTPAWMVERLARCGQRSVSALVDISNYVMFEFG
                     RPSHIFDLDKIHGALHVRWGRAGETLELLNGNTITVDENVGVIADERMVESLAGIMGG
                     QSTAVSDDTRNVYVEAAFWWPAAIAGRARRYNFATDAGHRFERGVDPALTVEHVERIT
                     RLIIEICGGEAGPMDDQVTGLPERKPVTLRVERAAKIIGMPITQAQCVDVLRRLGLSF
                     VEEAGRLHVTPPSWRFDLQIEEDLIEEVIRVLGYGGLPATPPLARIVARTRSESRRGP
                     HALRHALAVLDYQETINFSFVEERWEHDLAGNQQPIRLLNPIASQLSVMRSSLIGSLV
                     NALRFNLARRVSRVRLFEIGRVFMRDAGIADDLRSVAGVAQPVRVGGLAYGSADASQW
                     GSAERVVDFFDAKGDVEALLAPRQVRFVPAEHSAMHPGRCAAVELDGRVIGHVGELHP
                     RWRQAYELPQAPVVFELDAQALLERPLPSYAAIPRQQSVARDIAIVVDERVSHDRLMQ
                     VIAAAPTQGLLQAARLFDIYKPKQAGADIAAGERSLAIHLELLDEEVTLTDERIEAAK
                     QTVLDALARETGARLRA"
BASE COUNT          374 a          745 c          731 g          386 t
ORIGIN      
        1 ctcgctgacc tcaccgatgg tgaagtagcg cttggcagga atcgcaggaa gcgctttctc
       61 cattgaaatc aataagattg atggggaaag ctaacacttt actcgaagtt ttcctccgcg
      121 gatatatcgc cttgaacgac acccttgagc ttgtggctgg catgaaacgt tacgacgttg
      181 cgggccttga tgggtatggc ctcgccggtg cgcggattgc ggcccgggcg cggcgccttg
      241 cggcggatct ggaagttgcc gaagcccgag agcttcacgt ccttgccctg caccagggtg
      301 ccgtggacca gctcgaagaa ggcctcgacc atgtccttgg attcgcgctt gttcaagccc
      361 aggcgctcga acagcaggtc cgccagctcg gccttggtca gcgtgggcgt ttccagcgtc
      421 ggcaggatga ctctgtccat gggcttgtta ttgtcctgag tgctcattct tgtcggccct
      481 gcttaggcgc gcaggcgcgc gccggtttcc cttgccaagg catcgagcac ggtctgcttc
      541 gcggcctcga tgcgctcatc ggtcaaggtg acctcttcgt ccagcagttc gaggtgtatg
      601 gcgaggctgc gctcgcccgc cgcgatatcg gcacccgcct gcttgggctt gtagatgtcg
      661 aacagccgcg cggcctgcaa cagtccctgg gtgggcgcag cggcgatcac ctgcatcagc
      721 cgatcgtggc tgacccgctc atcgaccacg atggcgatgt cgcgcgccac cgattgctgg
      781 cgggggatcg cggcgtagct cggcaaaggc cgctccagca aggcctgggc gtcgagctcg
      841 aagacgacgg gcgcctgcgg caactcgtag gcctggcgcc agcgcggatg cagctcgccg
      901 acgtggccga tcacccgccc atcgagctcg accgcagcgc agcgccccgg atgcatggcg
      961 gaatgctcgg ccggcacgaa acgcacctgg cgcggtgcga gcaaggcctc gacgtccccc
     1021 ttggcatcga agaagtccac aacgcgctcg gcgctacccc attgcgaggc atccgcggag
     1081 ccataggcca agcctcccac gcgcaccggc tgggcgaccc cggccacgct gcgcaagtcg
     1141 tccgcgatgc cggcgtcgcg catgaacacg cggccgatct cgaacaggcg aacccgcgag
     1201 acgcgccgcg cgagattgaa gcgcagcgcg ttcaccagac tgccgatcag gctggaacgc
     1261 atcacgctca attggctggc gatggggttg agcagacgga tcggctgctg gttgccggcg
     1321 agatcgtgct cccagcgctc ctcgacgaag ctgaaattga tggtttcctg gtagtccagc
     1381 acggccagtg cgtggcgtaa ggcatgaggc ccgcgcctgg attccgaacg ggtgcgcgca
     1441 acgatccggg ccaggggtgg cgtggccggc agcccgccat agccgagcac gcggatcact
     1501 tcctcgatca ggtcttcctc gatctgcagg tcgaagcgcc agctcggcgg cgtgacatgc
     1561 aggcggcctg cctcctcgac gaagctcagg cccagacggc gcagcacatc gacacattga
     1621 gcctgggtga tgggcatgcc aatgatcttg gccgcgcgct ccacgcgcag cgtcaccggc
     1681 ttgcgctcgg gcaggccggt cacctggtcg tccattggcc cggcttcgcc gccgcagatc
     1741 tcgatgatca gccgcgtgat gcgctcgaca tgctcgaccg tcagcgccgg gtccacgccg
     1801 cgctcgaaac gatgcccggc atctgtcgcg aagttgtagc ggcgcgcacg gccggcgata
     1861 gcggccggcc accagaaggc ggcctcgaca tagacattgc gcgtgtcgtc cgacaccgca
     1921 gtggattgcc cgcccatgat gccggccagc gactcgacca tgcgctcgtc ggcaatcacg
     1981 cccacgttct cgtcgacggt gatggtgttg ccgttgagca gctccagcgt ctcgccggcg
     2041 cggccccagc gcacatgcag cgcgccgtgg atcttgtcga gatcgaagat atgcgacggc
     2101 cggccgaact cgaacatcac gtagttggag atgtccacca gcgcgctcac ggagcgctgg
     2161 ccgcagcgtg ccaggcgctc gaccatccac gccggcgtgc tcgccttggg attcacaccc
     2221 ttgatcacgc ggccgg
//
LOCUS       DASUCY010000140         4749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_982917, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000140 DASUCY010000000
VERSION     DASUCY010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4749
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982917"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..551
                     /locus_tag="VFV25_02770"
     CDS             90..551
                     /locus_tag="VFV25_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY1392268.1"
                     /translation="MSTSFTLNGKPVTVNADPSTPVLWTLRDHLGMTGTKFGCGAALC
                     GACTVHLNGQAIRSCITPISSVAGQSITTIEAQAHDKVGRAVQAAWQKHDVAQCGYCQ
                     SGQIMSATALLKSNRHPSDADIDGAMSGNICRCGTYVRIRAAIHDAAKALA"
     gene            563..2806
                     /locus_tag="VFV25_02775"
     CDS             563..2806
                     /locus_tag="VFV25_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY1392269.1"
                     /translation="MNYNALMQRFAAAQAAQAAQAAPADGASALGRRSFIKLGVASGF
                     ALGLFGLDAKAQEASAQAAGLKPSQQPSAFLRVEPNGDIVVLVNKLDFGQGVQTGLPM
                     LVAEELDADWSKVRGELAPAGDAYKDPNFGIQMTGGSTSTHNDWLPYRELGARARAMF
                     VAAAAQRFGVPAERVKLANGVASAGGKRATLGELAAEAMKQLVPEKVTLKEPKAWRLL
                     GKPTHRLDRVAKTTGTQDFGIDVKLPGLKVALVTRPPVFGAKLASYDASRARAVPGVV
                     EVLEVPLDRGASGLAVVAEGYWPAKQAREALELHWDTSGVEKPDSDKLFAQYRELARQ
                     PGMKAREADTSALASAPRKLVAEYSFPYLAHAPMEPLNCTLHYSGDHCRVWAGSQFQT
                     IDQATVAGVLGLKPEQVEFNTMMAGGGFGRRAVPTSDYIAEAAQVVKAWRAKGHGEPI
                     KLMWSREDDIHGGYYRPMHVHRAEIGYDEQGKVLAWKHTIVGQSILAGTPFESFMVKN
                     GIDAVTVEGVADTPYELPLSLDVHAPKVNVPVLWWRSVGNTHTAYVMETLIDELARGA
                     GKDPVAYRKALLGDKHPRHVAALDLAVAKSGYGKKQLPSGRAWGVALHESFGSVVAYV
                     VQASLDKGQPVLHEVTAGVHCNFAVNPLAVEAQVQGASIMGLGTTLPGAEITFKDGAI
                     QQNNFGEYPVPRISQAPKIAVYIVPSADPPTGMGEPGVPPLAPAFANAIAGLGGKRLR
                     DLPFKLT"
     gene            2928..3617
                     /locus_tag="VFV25_02780"
     CDS             2928..3617
                     /locus_tag="VFV25_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY1392270.1"
                     /translation="MSARAEIEAREGVDQAEEDRVTTGPVIVVLAAGSGSRFSGSSHK
                     LAQALGGASVLGVTLEHALASSLPVVVVTTHELAPVARQFVAARDMVLLPSVGSSSAE
                     PLGMGYSIASGVAARPHASAWLVLPGDMPLVQPSTMQAVARALDQHPVAYAQHLGRRG
                     HPVGFAAELYSELVTLSGDEGARRLLGRYPSYGVEVDDPGALLDVDTEDDLHALRLAQ
                     GDVGQGLQLQK"
     gene            complement(3625..4275)
                     /locus_tag="VFV25_02785"
     CDS             complement(3625..4275)
                     /locus_tag="VFV25_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003839 - gamma-glutamylcyclotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061928 - glutathione specific
                     gamma-glutamylcyclotransferase activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HEY1392271.1"
                     /translation="MLDDYANVSAPQAFQGLDGAMPAPLRDPHELFEATLRGWNPRED
                     LWVFGYASLIWRPEFDADEQRLARVHGYHRALRMRSRVNRGTPQTPGLVFALLSGGSC
                     QGMAFRVPRANADRELRRLWQREMPTGVYDPRWLHCRTAQGPVKALAFTLSRGSPNYT
                     GEIADEEMLAILREARGRYGTTLEYLLATATGLRECGICDAEIERLAALARQHGLA"
     gene            4362..>4749
                     /locus_tag="VFV25_02790"
     CDS             4362..>4749
                     /locus_tag="VFV25_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392272.1"
                     /translation="MTKDTGPNEKAEPAWRWLAGAAVLLLAGVLGYEIVSKESAGLPT
                     AGSAVEALGGQPTLIGEEVEIAASGEPLVVVYFELAQADVPLDAAQALESVLRAAEAD
                     AAARVLLSGYHDASGDAAKNADLARDR"
BASE COUNT          788 a         1579 c         1669 g          713 t
ORIGIN      
        1 cgagtgcaaa gtcccgcctt caccgatcca gccacacatc ggctgcagtc tggccgatgt
       61 catccgccac caagaaccgg agactcgcga tgagcacctc atttaccctc aacggcaagc
      121 cggtcacggt caatgccgat ccctccacgc cggtgctgtg gacgctgcgc gaccatctgg
      181 gcatgaccgg caccaagttc ggctgcggtg cggcactgtg cggcgcctgc accgtgcacc
      241 tcaacgggca ggcgatccgc tcctgcatca cgccgatttc ctcggtggca gggcagagca
      301 tcacgaccat cgaggcgcag gcccatgaca aggtcggccg tgcggtgcag gcggcctggc
      361 agaagcacga cgtcgcgcag tgcggctact gccagagcgg ccagatcatg agcgccacgg
      421 ccctgctcaa gtccaaccgc catcccagcg acgccgacat cgacggcgcc atgagcggca
      481 acatctgccg ctgcggtacc tatgtgcgca tccgtgccgc gatccacgac gcggccaagg
      541 ccctggcttg aggaggctgg tcatgaacta caacgcactc atgcaacgct ttgcagcggc
      601 tcaggcggct caggcggccc aggcggctcc ggccgacggc gcgtccgccc ttggccgccg
      661 cagcttcatc aagctcggcg tggccagcgg cttcgcgctc gggctgttcg gcctggacgc
      721 caaggcccag gaggcgtcgg cccaggctgc gggcctgaaa cccagccagc agcccagcgc
      781 cttcttgcgc gtggagccca acggcgacat cgtggtgctg gtgaacaagc tggatttcgg
      841 ccagggcgtg cagaccgggt tgccgatgct ggtggccgag gagctggacg ccgactggtc
      901 caaggtgcgc ggcgaactcg cgccggccgg cgatgcctac aaggatccga acttcggcat
      961 ccagatgacg ggcggctcga catcgaccca caacgactgg ctgccctatc gcgagctggg
     1021 cgcgcgcgcc cgcgcgatgt tcgtcgccgc ggcggcacag cgtttcggcg ttccggccga
     1081 gcgggtcaag ctcgccaatg gcgtggccag cgccggtggc aagcgggcca cgctgggcga
     1141 actcgcggcc gaggcgatga agcagctggt tcccgagaag gtcacgctga aggagcccaa
     1201 ggcctggcgg ctcttgggca agcccacgca tcgcctggac cgcgtcgcca agaccacggg
     1261 cacgcaggac ttcggcatcg acgtgaagct gcccggcctc aaggttgccc ttgtgactcg
     1321 cccgccggtg ttcggcgcca agcttgcgag ctatgacgcg agccgggcgc gtgcggtgcc
     1381 cggcgtcgtc gaggtgctgg aggtgccgct ggaccgcggc gccagcgggc tggccgtggt
     1441 ggccgagggc tattggccgg cgaagcaggc gcgcgaggcg ctcgagctgc actgggacac
     1501 gagcggcgtc gagaagcccg acagcgacaa gctcttcgcg caatatcgcg agctggccag
     1561 gcagccgggg atgaaggccc gcgaggccga cacctctgcg ctcgcctccg cgccgcgcaa
     1621 gctggtggcc gagtacagct tcccctacct cgcccatgcg ccgatggagc cgctcaactg
     1681 cacgctgcac tacagtggtg accattgccg cgtctgggcc ggctcgcagt tccagaccat
     1741 cgaccaggcg acggtggctg gcgtgctggg tctcaagccc gagcaggtcg agttcaacac
     1801 catgatggcc ggcggcggct tcgggcgccg cgcggtgccg acttccgact acatcgccga
     1861 ggcggcccag gtcgtgaagg cctggcgcgc caagggccat ggggagccga tcaagctgat
     1921 gtggagccgc gaggacgaca tccacggcgg ctactaccgg cccatgcatg tgcatcgcgc
     1981 cgagatcggc tacgacgagc agggcaaggt gctggcgtgg aagcacacca tcgtcggcca
     2041 gtccatcctc gccggcacgc ccttcgagag cttcatggtg aagaacggca tcgatgccgt
     2101 cacggtcgag ggcgtggccg acacgcccta tgagctgccg ctgtcactcg atgtgcatgc
     2161 gcccaaggtc aacgtgccgg tgttgtggtg gcgctcggtc ggcaacacgc ataccgccta
     2221 cgtgatggaa accctgatcg acgaactcgc gcgcggcgcg ggcaaggatc cggtcgccta
     2281 tcgcaaggcc ctgctagggg acaagcatcc gcgccatgtg gcggcgctgg atctggccgt
     2341 cgctaaatcc ggctatggca agaagcagct gccctcaggg cgggcctggg gtgtggcgct
     2401 gcacgagtcc ttcggctcgg tcgtggccta tgtggtgcag gcttcgctgg acaagggcca
     2461 accggtgctc cacgaagtga cggccggcgt gcattgcaac ttcgcggtca atccgctggc
     2521 ggtcgaggcg caggtgcagg gcgcctcgat catgggcctg ggcacgacgc tgccgggtgc
     2581 ggaaatcacc ttcaaggacg gcgcgatcca gcagaacaat ttcggtgaat accccgtccc
     2641 gcgcatcagc caggcgccca agatcgccgt gtacatcgtg cccagcgccg atccgcccac
     2701 cggcatgggc gaacccggcg tgccgccgct ggcgccggcc ttcgcgaatg ccatcgccgg
     2761 cctcggcggc aagcgcctgc gcgacctacc tttcaaattg acgtgagctt gacgcactga
     2821 tatgcaaaga aaatcccgcc ggctcgttgc caaacgcaac agccgcgcgg gaaacacctt
     2881 aagtgatttc cgccttccgc gaagcggccg gctgcgctcc catatgcatg tcggcgcgcg
     2941 cggaaatcga ggcgcgcgaa ggggtagatc aagcggagga ggaccgggtg acaacagggc
     3001 cggtcatagt cgttctggcc gcgggcagcg gctcgagatt tagcggcagc agccacaagc
     3061 ttgcgcaagc tctgggcggc gcgagtgtat tgggcgtgac gctcgagcat gcgttggcca
     3121 gcagcctgcc ggtggtcgtc gtgacgaccc acgaactggc gccggtcgcg cgccagttcg
     3181 tcgcggccag ggacatggtg ctcctgccga gcgtcggctc cagcagcgcc gagcctctag
     3241 gcatggggta ttcgattgcc agcggggttg cggcgcggcc tcatgccagc gcctggctgg
     3301 tgctgccggg agacatgccg ctggtgcagc catccacgat gcaggcagtg gcgcgcgcgc
     3361 tggaccagca tcccgtggcc tatgcgcagc atctgggccg gcgcggccac ccggtcggtt
     3421 tcgcggccga gctgtattcc gagctggtca cgctgagcgg cgacgaaggc gcgcgccggc
     3481 tgttggggcg ctacccctca tacggcgtcg aggtggacga ccccggcgcg ttgctggacg
     3541 ttgacaccga ggacgacctg cacgcgctgc gcctggcgca gggcgacgtc gggcaagggc
     3601 tgcaactgca gaagtagtgc agcctcacgc caggccgtgc tgcctggcca aggctgccag
     3661 gcgctcgatc tccgcatcgc agatgccgca ctcgcgcagg ccggtggccg tggcgagcag
     3721 gtactccagc gtcgtgccat agcgaccgcg cgcctcgcgc aggatggcga gcatctcctc
     3781 gtccgcgatc tcccctgtgt aattcgggct gccgcggctc agcgtgaagg ccaaggcctt
     3841 gaccggcccc tgggcggtac ggcaatgcag ccagcgcggg tcgtagacac cggtgggcat
     3901 ctcgcgctgc cacaggcgcc tgagttcccg gtctgcattg gcgcgcggca cgcgaaaggc
     3961 cattccctgg caggagccgc cgctcaacag cgcgaacacc aggcccggcg tctgcggcgt
     4021 gccgcggttg acgcgcgagc gcatgcgcaa cgcccggtga tagccgtgca cgcgtgccag
     4081 gcgctgctcg tcggcatcga actcggggcg ccagatcagc gaggcatagc cgaacaccca
     4141 caagtcttct ctcgggttcc agccgcgcaa ggtggcctcg aagagttcgt gaggatcgcg
     4201 cagcggcgcc ggcatggcgc catcgaggcc ctggaaggct tgcggcgcgc tgacgtttgc
     4261 atagtcatca agcatgaagc gcagcgtgct ttgcgccggg cttgactgtc aagatgcacg
     4321 gcgacgatag tcggaacgat gcgacatgcg caggaacgcc aatgacgaag gacaccgggc
     4381 ctaacgaaaa agcggaaccg gcatggcggt ggttggcggg cgccgccgtc ttgctgctgg
     4441 ccggcgtgtt gggctatgag attgtcagca aggagagtgc gggcctgccg accgccggct
     4501 cggcggttga agcgctgggg ggccagccca ccctcatcgg cgaggaggtc gagatcgccg
     4561 cgagcggcga gcctcttgtc gtggtgtatt tcgagctggc ccaggccgat gtgccgctcg
     4621 acgcagcgca ggccctggag agcgtgctgc gcgccgccga ggccgatgcg gcggccaggg
     4681 tgctgctgtc gggctatcac gatgccagtg gcgacgcggc gaagaacgcc gatctggccc
     4741 gcgaccggg
//
LOCUS       DASUCY010000141         2067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_613472, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000141 DASUCY010000000
VERSION     DASUCY010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613472"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VFV25_02795"
     CDS             <1..241
                     /locus_tag="VFV25_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEY1392273.1"
                     /translation="VAIVLGANDVVNPAALQKGSPIYGMPILEAYKAKTVIVNKRSMA
                     AGYAGLDNELFYMDKTMMVFGDAKKVVEDAVKAIE"
     gene            251..1219
                     /locus_tag="VFV25_02800"
     CDS             251..1219
                     /locus_tag="VFV25_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY1392274.1"
                     /translation="MKHATLRQLKVFESVARHLSCSRAAEELHLTQPAVSTQIGKLEE
                     HAGNLLFEQFGKKVYLTAAGAELLQISRAIIQQFEQAESAMAQFRGVCGGRLNVGVIS
                     AGDYFFPRLLVEFIGRHSGVTLNFTVHNREELLAHIAENQTDLAVMVRPPTELDTMNQ
                     PFAPHPYVIVAPSEHPMVDRRRIPLVEAMRQPFVVRERGSDTWRSMCEGFGGNLSDLN
                     VAMEIRSTETIKQAVIAGLGLSFLSAHTVSRELQSGSLRVLDVQGFPLRLHWYVVHRR
                     TKRLPPVAQAFKDFLLSEGASLIARAVPGEVQRSVPEGVEHRAPAI"
     gene            1614..>2067
                     /locus_tag="VFV25_02805"
     CDS             1614..>2067
                     /locus_tag="VFV25_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392275.1"
                     /translation="MLLLIGIAIGAGGVIVVQERYLPPRLSADASAKLSSAFVQADAQ
                     RLRLKTELSETTKRLDTALADRKSLADELAASRAAAERLGKDVASVVASLPPDPRGGG
                     RGSVEVRAGQFTAKGGMLAYDVVLTRERATGKPITGVMQLAVSGEAARG"
BASE COUNT          361 a          681 c          683 g          342 t
ORIGIN      
        1 cgtggccatc gtgctgggcg ccaacgacgt ggtgaacccg gccgcgctgc aaaagggcag
       61 cccgatctac ggcatgccca tcctggaggc ctacaaggcc aagacggtga tcgtcaacaa
      121 gcgctccatg gccgccggct acgcggggct ggacaacgag ctgttctaca tggacaagac
      181 catgatggtc ttcggcgacg cgaagaaggt ggtcgaggac gccgtcaagg ccatcgagta
      241 gggcctggcc gtgaagcacg cgacgctgcg ccagctcaag gtcttcgagt cggtggcccg
      301 gcacctcagc tgttcgcgtg ccgccgaaga gcttcacctg acgcagcccg cggtttccac
      361 gcagatcggc aagctcgagg agcatgccgg caatctgctg ttcgagcagt tcggcaagaa
      421 ggtgtatctc accgccgccg gcgccgagct gctgcagatc agccgcgcca tcatccagca
      481 gttcgagcag gccgagagcg ccatggcgca gttcaggggc gtgtgcggcg gccgcctcaa
      541 cgttggcgtg atcagcgcgg gcgactattt ctttccgcgc ctgctggtgg agttcatcgg
      601 ccgccacagc ggtgtcacgc tcaacttcac ggtgcacaac cgtgaggagc tgctggccca
      661 catcgccgag aatcagaccg acctcgccgt catggtgagg ccgcccacgg agctggacac
      721 gatgaaccag ccattcgcgc cgcaccccta cgtcatcgtg gccccatccg agcaccccat
      781 ggtcgaccgg cggcgcattc ccctggtgga agcgatgcga cagcccttcg ttgtgcgcga
      841 gcgcggctcc gacacctggc gctcgatgtg cgagggcttc ggcggcaatt tgtcggacct
      901 caacgtggcc atggagatcc gcagcaccga gacgatcaag caggccgtga tcgccggctt
      961 aggcctgagc ttcctgtcgg cccacacggt gagccgggaa ctacagtcgg gcagcttgcg
     1021 agtgctggac gtgcagggct tcccgctgcg gctgcactgg tacgtggtgc atcggcgcac
     1081 caagcgcctt ccgcccgtgg cgcaggcgtt caaggacttc ctgctcagcg agggtgcatc
     1141 gctgatcgcg cgggctgtac cgggggaggt gcagcgttcg gtgccggaag gcgtcgagca
     1201 ccgcgcaccc gcgatttgat caggcacatg cggcacgtgc tgtgacagcg gcacgcgact
     1261 cccagccgca gcctgtactc acggtcttgg ccacattgcc gacgtccaag gggtgccgcg
     1321 cgacgccacc tggggcgaac gctacttcca aattaccgac ctagatggcc acgaactgag
     1381 cttcgccagc cccctgggca agtccacgca acttgggcgc gacacgtgaa ttccatcagc
     1441 ctgcccgcca atccaggcgc ggtacatggc gtttcaaatc gtgtcttcgc gggttgtacc
     1501 ctgtcgcatc gcttctcagc ccatcgacac atcaaatgct cggccaatca aaacccatcc
     1561 ctttcgagcc ttatggaaga cggcgctcgc gctggcgctt gccgcgatgg cttgtgctcc
     1621 tgctgatcgg gatagcgatc ggcgctggcg gcgtgatcgt cgttcaggag cgctatctgc
     1681 cgccgcgctt gtcggccgat gcgtcggcga agctgagcag cgcgttcgtg caggccgatg
     1741 cgcagcggct gcgactgaag actgaactca gtgagaccac gaagcggctt gacaccgctc
     1801 tcgccgacag gaagagcctg gccgacgagc tcgccgcgag ccgtgcagcg gccgaacgtc
     1861 ttggcaagga cgtcgcatcg gtggtcgcat cgctgccgcc cgatccgcgc ggtggtggtc
     1921 gcggcagcgt cgaagtgcgc gcggggcaat ttacggccaa gggcggcatg ctcgcctacg
     1981 acgtggtgct cacgcgcgag cgcgcaacgg gcaagcccat caccggcgtg atgcaactcg
     2041 cggtgagcgg cgaggccgca cgcggcg
//
LOCUS       DASUCY010000142         3358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_645744, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000142 DASUCY010000000
VERSION     DASUCY010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645744"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="VFV25_02810"
     CDS             <1..642
                     /locus_tag="VFV25_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003112741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEY1392276.1"
                     /translation="LNALTGLAFRPGDDYFALMGSQDAAVALSGQLKTTAVSLMKIAN
                     DLRWMNCGPLAGLAEIELPALQPGSSIMPGKVNPVIPEATAMVAAQVIGNDAAITVAG
                     QSGNFELNVMLPLIAHNLLGSLQLLARASRLLADKAIAGFKVNEVQLQAALARNPILV
                     TALNPLIGYLKAAEIAKQAYAQGRPIIDVADELTDLGRERLQELLDPRKLTGG"
     gene            814..2853
                     /locus_tag="VFV25_02815"
     CDS             814..2853
                     /locus_tag="VFV25_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEY1392277.1"
                     /translation="MYALFEDAGKFLAGRVMSEAESSMQVELDSGKRVKVKSANVLIR
                     FAKPAPAEVLTRGQQLAHEIDLDLIWEVAPDEEFGFEDIAREYFSAQADAPQQAAALF
                     RLFEAPHYFHRRGKGRFRKASEDVLKQALAAIERKARQAEQIDAWAAELADGQCPASI
                     REQLYKLLFKPDKNAPEYKAVVEAAKRTHKAPLDLLKDAGAIDSPYQFHWKRFLFEFF
                     PKGTGFPALSAPPIKDELPLAATPAFSIDDSATTEIDDALSVRGLGTGTVVLGVHIAA
                     PALAIAPGSPLDAVARTRMSTVYMPGYKLTMLPDAVVESYTLQEGRDCPAVSIYFTLD
                     EATLTIQASETKLERVPIAANLRHDKLDAVITEAKLTGAQAADYPFAGELTFAFRLAR
                     HLKAQRELVRGKPENFNRPDYNFKLDGNGNAEPDGSERVLIGLRQRGAPLDLIVSETM
                     ILANSSWGALLADCGVPGIYRSQASLAPGIKVRMGVKPAPHAGMGVKQYTWATSPLRR
                     YVDLVNQWQIVAAARHGRTAALVAPFKPKDAELFSVISGFDAAYSAYNGFQNGMERYW
                     TLRYLEQNGITELDAAVMKDGLVRADALPLVFRALGTEALPRGAHVRVRITGTDLLTL
                     DVHANLMARLDADEAAAAAPEADEGEEAEAAGPLTLAIDVQDAEAGEGAAAAVAP"
     gene            2871..>3358
                     /locus_tag="VFV25_02820"
     CDS             2871..>3358
                     /locus_tag="VFV25_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY1392278.1"
                     /translation="MSWLKKLSTLQIALLASFSVHAVLLAVRIVDPEDFNRVFEDSPL
                     EVILVNAGSKERPAKAQAIAQRNLVGGGEAEQGRATSPLPPSALTEVGDAMEETHRQI
                     EQMIDEQAQLLAQVKQELAQLPPPDPKHDSGNPDARNQDEKRRQLLRLLAEIEKRINE
                     ENA"
BASE COUNT          581 a         1163 c         1136 g          478 t
ORIGIN      
        1 ctgaacgcgc tcaccggctt ggccttccgg cccggcgacg actacttcgc gctgatgggt
       61 tcgcaggatg cggcggtcgc actgtcgggc cagctcaaga cgacggcggt gtcgttgatg
      121 aagatcgcca acgatctgcg ctggatgaac tgcgggccgc tggccggcct ggcggagatc
      181 gagctgccgg cgctgcagcc tggctcctcc atcatgccgg ggaaggtcaa cccggtgatc
      241 ccggaagcga cggcgatggt ggcggcccag gtgatcggca acgacgccgc catcacggtg
      301 gccggccaga gcggcaactt cgagctcaat gtgatgctgc cgctcatcgc gcataacctg
      361 ctgggcagcc tgcagttgct ggcgcgcgcg agccggttgc tggccgacaa ggcgatcgcc
      421 ggattcaagg tcaacgaggt gcagctgcag gccgcgctgg cgcgcaaccc catcttggtg
      481 acggcgctga acccgctgat cggctacctc aaggccgccg agatcgccaa gcaggcctat
      541 gcgcagggcc ggcccatcat cgatgtggcc gacgagctga ccgatctggg acgtgagcgc
      601 ctgcaggaac tgctcgatcc tcgcaagctg accggaggat gacagcccct cgtccggcga
      661 gtacgccggc cagccttgca ggccgcgctg gccgcaagcg tccagccctt cgccaggcac
      721 aaacagtccc ccggactgcg gcccggcgcg gcggcccgag ggcagcccct cagccgacgc
      781 ccacgcaagg gcatcggcga caatcacgcg ggtatgtatg ccctgttcga agacgccggc
      841 aagttcctgg ccggccgcgt gatgtcggag gccgagagct cgatgcaagt cgagctcgat
      901 tccggcaagc gcgtcaaagt gaaatccgcc aatgtgctga tccgcttcgc caagccggcg
      961 ccggcggagg tgctgacgcg aggccagcag cttgcgcacg agatcgacct cgatctgatc
     1021 tgggaagtcg cacccgacga ggagttcggc ttcgaggaca ttgcgcgcga atacttcagc
     1081 gcccaggccg atgccccgca gcaggcggcg gcgctgtttc gcctgttcga ggcgccgcat
     1141 tacttccacc gccgcggcaa gggacgcttc cgcaaggcgt ccgaggacgt gctgaagcag
     1201 gccctggccg cgatcgagcg caaggctcgc caggccgagc agatcgacgc ctgggcagct
     1261 gagctggccg atgggcagtg cccggcgagc atccgcgagc agctctacaa gctcttgttc
     1321 aagcccgaca agaacgcgcc cgaatacaag gccgtggtcg aggcggccaa gcgcacgcac
     1381 aaggcgccgc tggacctgct gaaggacgcc ggcgcgatcg acagccctta ccagttccac
     1441 tggaagcgct tcctgttcga gttcttcccc aagggtacgg gcttcccggc gctcagcgcc
     1501 ccgccgatca aggacgagct gccgctggcc gcgacgccgg ccttctcgat cgacgattcc
     1561 gccaccacgg agatcgacga tgccttgtcg gtgcggggcc tgggcacggg gacggtcgtc
     1621 ctgggcgtgc acatcgccgc gcccgcgctg gccatcgccc cgggctcgcc tctcgacgcg
     1681 gtggcgcgca cgcgcatgtc cacggtctat atgccgggct acaagctcac catgctgccc
     1741 gacgcggtcg tggagagcta cacgctgcaa gaaggccgcg actgcccggc cgtgtcgatc
     1801 tatttcacgc tcgacgaggc gacgctcacg atccaggcca gcgagaccaa gttggagcgc
     1861 gtgccgatcg cagccaatct gcgccacgac aagctggacg ccgtgatcac cgaggccaag
     1921 ctcaccggtg cgcaagccgc ggactatccc ttcgctggcg agctgacctt cgccttccgc
     1981 ctcgcgcggc acctgaaggc gcagcgcgag ctcgtgcgcg gcaagcccga gaacttcaac
     2041 cggcccgact acaacttcaa gctcgatggc aacggcaatg ccgagcccga tggcagcgag
     2101 cgcgtgctga tcggcctgcg ccagcgcggc gcgccgctgg acctcatcgt ctcagagacg
     2161 atgatcctcg ccaacagctc ctggggcgcg ctgctggccg attgcggcgt gcccggcatc
     2221 taccgcagcc aggccagcct ggcgcccggc atcaaggtgc gcatgggcgt gaagccggcg
     2281 ccgcacgccg gcatgggcgt gaagcaatac acctgggcca cctcgccgct gcgccgctat
     2341 gtggacctgg tcaaccaatg gcagatcgtc gctgctgcgc gccatggccg caccgcggcg
     2401 ctggtggcgc ccttcaagcc caaggacgcc gagctgttct cggtgatctc gggcttcgat
     2461 gctgcctaca gcgcctacaa cggcttccag aacggcatgg agcgctactg gacgctgcgc
     2521 tatctggagc agaacggcat caccgagctc gatgccgccg tgatgaagga tggcttggtg
     2581 cgcgccgacg cgctgccgct ggtgttccgc gcgctgggca ccgaggcgct gccgcgcggc
     2641 gcgcatgtgc gcgtgcgcat caccggcacc gatctgctga cgctggacgt gcacgccaac
     2701 ctgatggcgc gcctggacgc cgatgaggca gccgccgcgg cgccagaagc cgacgagggc
     2761 gaggaagcag aggccgccgg cccgctcacg ctggccatcg acgtgcagga tgccgaggcc
     2821 ggcgaaggcg ccgcagcagc cgtcgcgccc tgaggatagt ggccccatcc atgtcctggc
     2881 tcaagaagct cagcaccctg cagatcgccc tgctcgcttc ctttagcgtg catgcggtgc
     2941 tgctggccgt gcgcatcgtc gaccccgagg acttcaaccg cgtgttcgaa gactcgccgc
     3001 tggaagtcat cctggtcaat gccggctcca aggagaggcc ggccaaggcg caggccatcg
     3061 cgcagcgcaa cctcgtgggc ggcggcgagg ccgagcaggg acgcgccacc tcgccgctgc
     3121 cgccctcggc gctgaccgag gtgggcgatg cgatggagga gacgcaccgc cagatcgagc
     3181 agatgatcga cgagcaggcc cagctgctgg cgcaggtgaa gcaggagctg gctcagctgc
     3241 cgccgcccga cccgaagcac gacagcggca atcccgatgc gcgcaaccag gacgagaagc
     3301 gccggcagct gctgcgcctg ctggccgaga tcgagaagcg catcaacgaa gagaacgc
//
LOCUS       DASUCY010000143         2403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_292334, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000143 DASUCY010000000
VERSION     DASUCY010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292334"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..99)
                     /locus_tag="VFV25_02825"
     tRNA            complement(23..99)
                     /locus_tag="VFV25_02825"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(63..65),aa:Gln,seq:ttg)
     gene            complement(152..1075)
                     /gene="ispE"
                     /locus_tag="VFV25_02830"
     CDS             complement(152..1075)
                     /gene="ispE"
                     /locus_tag="VFV25_02830"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEY1392279.1"
                     /translation="MSLRALYDVPAPAKLNLFLHVIGRRADGYHLLQSVFALIDWADI
                     LHFERRDDARIARHDLGAALPEDDLCLRAARALQLASGSPRGVDISIAKRVPWGAGLG
                     GGSSDAASTLLALNRLWGLNWPRKRLQALAVKLGADVPFFVGGRNAWVEGIGEQLTPI
                     ELAPRRYAVLKPAAVIPTAAIFASPLLARDSAPAILSDFLADPPRFLSGVGRNDLQLA
                     AQAASSEVAQALALLASRYGASRMTGSGSAVFADAGAEAGADESGFAGKSATGDAPVA
                     PMFEPHELPSGWVGRMCRGLERHPLVGWCAD"
     gene            complement(1092..1613)
                     /locus_tag="VFV25_02835"
     CDS             complement(1092..1613)
                     /locus_tag="VFV25_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HEY1392280.1"
                     /translation="MSLRALLASCVLVALVGGCATPSKLQAPIEGDHLSGRLALKVEG
                     DSARSFSAAFELDGGPALGQLSLTSPLGTRVAEARWAPGSAVLANSDGKRVYADLQDL
                     SRDALGESVPLVALFDWLQGRPWSGAPAEAREGGFEQLGWRIDLQRQAEGLIVVTRSV
                     PTLVSLRVKLDSK"
     gene            complement(1648..>2403)
                     /locus_tag="VFV25_02840"
     CDS             complement(1648..>2403)
                     /locus_tag="VFV25_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY1392281.1"
                     /translation="QVYLLLAQAAEQRRDYKAAEAWLNKIDNPAAALLVQSRRAAILA
                     QQGKLAEARELIRKTPERSPDDARAKLVAEAELLRDTRHWREAYDTLGEAVKRTPEDT
                     DLLYEQAMMAEKLDRMDDMERLLRRVIALKPEHQHAYNALGYSLADRNLRLPEARQLI
                     VKALQLAPGDPFITDSLGWVEYRMGNRAEAARLLRQAYEAQPDAEIAAHLGEVLWVSG
                     ERDEARKVLREGRDKDAGNDVIKDTLARLKVDL"
BASE COUNT          408 a          833 c          800 g          362 t
ORIGIN      
        1 cgctcagaaa gaagaagagg tttggcaggg gaggaaggac tcgaaccctc gcatgtcgga
       61 atcaaaatcc gatgccttaa ccaacttggc gactccccta cacaggaccc ggcccgaact
      121 gcagccatgc cctcaaaaaa acaatgtcgc ctcagtctgc acaccatccg accagcggat
      181 gccgctccag accgcggcac atgcggccca cccagcccga tgggagctca tgcggctcga
      241 acatcggggc gacgggcgcg tcgcctgttg ccgattttcc tgcaaacccg ctctcatcgg
      301 cgcccgcctc tgcgcccgca tctgcgaaca ccgcgctgcc cgagcccgtc atgcggcttg
      361 cgccgtagcg ggatgccagc agcgccagag cctgggcgac ctcgctgctt gcggcttgcg
      421 ccgcaagttg cagatcgttg cggcccaccc ctgagagaaa acgcgggggg tctgcaagaa
      481 agtccgacag tatagcaggg gcgctatcgc gcgccaagag cgggctggca aaaatcgccg
      541 cggtcgggat caccgcagcc ggcttcagca ccgcataacg gcgcggagcg agctctatcg
      601 gcgtgagctg ctcgcctatg ccctcgaccc aggcgttgcg ccccccgacg aagaagggca
      661 cgtccgcacc cagcttcacg gccagggcct gcaggcgctt gcgcggccag ttcaggcccc
      721 agagccggtt cagggcgagc aaggtgctgg cagcgtccga gctgccgccg cccaggcccg
      781 cgccccaggg cactcgcttg gctatggaga tgtcgacgcc ccgagggctg ccgctggcca
      841 gctgcagggc cctagcagct cgcaggcaga gatcgtcctc cggcaaggcg gcgccgagat
      901 cgtggcgcgc gatgcgcgcg tcgtcgcggc gctcgaaatg caggatgtcg gcccagtcga
      961 tcaaggcgaa cacggactgc agcaggtgat agccgtcggc gcggcgcccg atcacatgca
     1021 ggaagagatt cagcttggcc ggggcgggca cgtcgtaaag ggcacgcaag ctcatgagat
     1081 gccgtgggag atcatttcga gtcaagcttg accctcaggc tcaccagcgt cggcacgctg
     1141 cgcgtcacga cgatgaggcc ctcggcttgg cgctgcaggt cgatgcgcca gcccagctgc
     1201 tcgaagccgc cctcacgggc ctctgccggc gcgcccgacc agggccggcc ctgcagccaa
     1261 tcgaagagcg cgaccagcgg cacgctctcg cccagggcat cgcgcgagag gtcctgcaga
     1321 tcagcgtaga cgcgcttgcc gtcggaattc gcgagcaccg cgctgcctgg cgcccagcgc
     1381 gcctcggcca cccgcgtgcc cagcggactg gtcagtgaga gctggcccaa ggcgggtccg
     1441 ccgtcgagtt cgaaggcagc cgagaaggat cgggcgctgt cgccctcgac cttgagcgcc
     1501 aagcgcccgc tcaagtggtc gccttcgatc ggggcctgca gcttggacgg ggtcgcgcag
     1561 ccgcccacca gggccaccag cacgcaggag gccagcaacg cgcgcaggct caagagacaa
     1621 cctcggcgat catcaacccg agcccaatca aagatcgacc ttcaaccggg ccaaggtgtc
     1681 cttgatgacg tcgttgccgg cgtccttgtc acggccttcg cgcaggacct tgcgggcctc
     1741 gtcgcgctcg ccgctgaccc acagcacctc gcccagatgg gcggcgatct cggcatcggg
     1801 ctgagcctcg taagcctggc gcagcagacg cgcggcttcg gcgcggttgc ccatgcggta
     1861 ttcgacccag cccaggctgt cggtaatgaa ggggtcgcca ggcgccagct gcaaggcctt
     1921 gacgatgagc tggcgcgcct cgggcaggcg caggttgcga tcggccaggc tgtagccgag
     1981 cgcgttgtag gcatgctggt gctcgggctt gagcgcgatc acgcggcgca gcagcctttc
     2041 catgtcgtcc atacgatcga gcttctcggc catcatggcc tgctcgtaga gcagatcggt
     2101 gtcctcgggt gtgcgcttca cggcctcgcc cagcgtgtca taggcctcgc gccaatggcg
     2161 cgtgtcgcgc agcagctcgg cctcggcgac cagcttggcg cgcgcatcgt ccgggctgcg
     2221 ctcgggcgtc ttacggatca gctcccgcgc ctcggccaac ttgccttgct gggccaggat
     2281 ggcggctcgg cgcgactgca ccaacagcgc ggcagccggg ttgtcgatct tgttcagcca
     2341 ggcttcggca gccttgtaat cgcggcgttg ctcggcggcc tgggccagca gcagatagac
     2401 ctg
//
LOCUS       DASUCY010000144         2000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_886713, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000144 DASUCY010000000
VERSION     DASUCY010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886713"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1960)
                     /locus_tag="VFV25_02845"
     CDS             complement(<1..1960)
                     /locus_tag="VFV25_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY1392282.1"
                     /translation="MLANLNAEQLAAVTLPAGNALILAGAGSGKTRVLTTRIAWLLQT
                     GQVSPGGILAVTFTNKAAKEMQTRLSSMLPINTRGMWIGTFHGLCNRMLRAHFRDAGL
                     PQTFAILDSQDQLSAIKRLLKSLNVDDEKYPAKNLQYFINNAKEQGLRPADVEANDDF
                     NKRFVDLYAAYDTQCQREGVVDFAELLLRCYELLRYNDAIRAHYQRRFKHILVDEFQD
                     TNKLQYAWLKILAGHGEPGRSEDRPNAIFAVGDDDQSIYAFRGANVGNMVDFEREFRV
                     DHRIKLEQNYRSHGHILDTANHLIAHNTRRLGKNLRTDAGHGEPVRVYQAATDGQEAG
                     WIVDEIRERIASGSARSEIAILYRSNAQSRVIEHALFSAGIPYRVYGGLRFFERAEIK
                     HALAYMQLLENPRNDAAFGRVVNFPARGIGARSLEVLQDMARQYDCALAEAVAYVPGA
                     AGSKLAAFTRLIDKMREDTRRMTLAETVEYVINHSSLITHYQGEKEGQDRIENLQELV
                     TAAQAFVTEEGYGLEALAAVIPVGFESRVAQAVPQLAEGDQAQQVLDPEALPVVMTPL
                     VAFLTHASLEAGDNQAQAGQDAVQMMTVHAAKGLEFNVVFITGLEEGLFPHENSAQES
                     DGLEEERRLAYVAITRAKERLYLSFAQSR"
BASE COUNT          288 a          654 c          691 g          367 t
ORIGIN      
        1 tgcggctctg cgcaaacgac agatagagcc gctccttcgc ccgcgtgatc gccacatagg
       61 cgagccgccg ctcttcctcc agcccgtccg attcctgcgc gctgttctcg tgcgggaaca
      121 ggccctcttc cagcccggtg atgaacacca cgttgaactc caggcccttg gcggcgtgca
      181 cggtcatcat ctgcaccgcg tcctggccgg cctgggcctg gttgtcgccg gcctccagcg
      241 aggcgtgggt caggaaggcc accaggggcg tcatcaccac cggcagcgct tccgggtcca
      301 gcacctgctg ggcctggtcg ccttcggcca gctgcggcac ggcctgcgcc acgcgcgact
      361 cgaagccgac ggggatcacc gccgccagcg cctccagccc gtagccctct tccgtgacga
      421 acgcctgcgc ggcggtcacc agttcctgca agttctcgat gcggtcctgg ccttcctttt
      481 caccctggta gtgggtgatc aggctgctgt ggttgatcac gtactcgacg gtctcggcca
      541 gcgtcatgcg gcgcgtatcc tcgcgcatct tgtcgatcag gcgcgtgaac gccgccagct
      601 tgctgccggc cgcgccgggc acgtaggcca cggcctcggc gagcgcgcag tcgtactggc
      661 gcgccatgtc ctgcagcact tccagcgagc gcgcgccgat gccgcgcgcc gggaagttga
      721 ccacgcggcc gaaggcggca tcgttgcgcg gattctccag cagctgcatg taggccagcg
      781 cgtgcttgat ttcggcgcgc tcgaagaagc gcaggccgcc gtacacgcgg tacgggatgc
      841 cggccgagaa cagcgcatgc tcgatcacgc gcgactgcgc gttgctgcgg tacaggatgg
      901 cgatctcgga gcgcgccgag ccgctggcga tgcgctcgcg gatttcatcg acgatccagc
      961 cggcctcctg cccatcggtg gcggcctggt agacgcgcac cggctcgccg tggccggcgt
     1021 cggtgcgcag gttcttgccc aggcggcgcg tgttgtgcgc gatcaggtgg ttggccgtat
     1081 cgaggatgtg gccatgcgag cggtagttct gctccagctt gatgcggtgg tcgacgcgga
     1141 attcgcgctc gaaatcgacc atgttgccga cgttggcacc acggaaggcg tagatgctct
     1201 ggtcgtcgtc gcccacggcg aagatggcgt tgggccgatc ttccgacctg cccggctcgc
     1261 cgtggccagc gagaatcttc agccaggcgt attgcagctt gttcgtatcc tggaactcgt
     1321 ccaccaggat gtgcttgaag cgccgctggt aatgcgcgcg gatggcgtcg ttgtagcgca
     1381 gcagctcata gcagcgcagc agcagttcgg cgaagtcgac cacgccctcg cgctggcact
     1441 gcgtgtcgta ggcggcgtag aggtcgacga agcgcttgtt gaagtcgtcg ttggcctcga
     1501 cgtcggccgg gcgcaggccc tgctccttgg cgttgttgat gaagtactgc aggttcttgg
     1561 ccgggtactt ctcatcgtcg acgttgagcg acttcaacag ccgcttgatc gccgaaagct
     1621 ggtcctggct gtccaggatg gcaaaggtct ggggcaggcc cgcgtcgcgg aaatgcgcgc
     1681 gcagcatgcg gttgcacagg ccgtggaagg tgccgatcca catgccgcga gtgttgatcg
     1741 gcagcatcga cgacagccgc gtctgcattt ccttggcggc cttgttggta aaggtcaccg
     1801 cgaggatgcc gcccggcgag acctggccgg tctggagcag ccacgcgatg cgtgtggtca
     1861 gcacccgggt cttgcccgag ccggcgccgg cgaggatcag cgcattcccg gcaggaagcg
     1921 tcacggctgc cagttgctcc gcgttcaggt tggcaagcag gggggacgcg gtgggcgcct
     1981 ggcccgcggc gtcgggcgcg
//
LOCUS       DASUCY010000145         2213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_565470, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000145 DASUCY010000000
VERSION     DASUCY010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565470"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VFV25_02850"
     CDS             complement(<1..579)
                     /locus_tag="VFV25_02850"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEY1392283.1"
                     /translation="MALIVHKYGGTSMGSTERIRNVAKRVAKWHRAGHQMVVVPSAMS
                     GETNRLLALAKELSPGSQTPELARELDMIAATGEQVSSGLLSIALQSEGLQAVSYAGW
                     QVPVKTDSSYTKARIQSIDDARVRADLEAGKVVVITGFQGIDDDGHITTLGRGGSDTS
                     AVAVAAAMKADECLIYTDVDGVYTTDPRVVPEA"
     gene            complement(673..1998)
                     /gene="tilS"
                     /locus_tag="VFV25_02855"
     CDS             complement(673..1998)
                     /gene="tilS"
                     /locus_tag="VFV25_02855"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEY1392284.1"
                     /translation="MRQTAVVGIAYSGGRDSTALLHATLKAAQAQGLSVAALHVHHGL
                     SPQADAWLAHCEAQCRRWARQGLPVSLHFERLHLHLRAGDSVEAVAREARYAALRRLA
                     LAAGAEAVLLAHHLRDQAETFLLQALRGAGVAGLAGMPAAIERGGIAWLRPWLKLPRE
                     AIEAYVRLHRLNHIDDDSNEDTRFARNRLRLEVWPQLSAAFPQAEAKLADAASWAQEA
                     NACLSDLAALDLSKLVKPQGLDIDAWSGLPAHRARNALRAWVRECSGEALPAAQLERL
                     YAELPRGRGPARWALNGGWLRRYRGCLSFVEHDRPSEAASTQPREASVSIRRAGRYAL
                     PGWGGSLLVKRVAERGVPLARLGELALLPRSGGEQFQPEPGRPARSLKKQYQAAAVPA
                     WSREGPLAYVAGQLVFVPGLGIDARAWGEKGQAMVALEWLAKKPAGNLR"
BASE COUNT          328 a          793 c          755 g          337 t
ORIGIN      
        1 cgcctcgggc accacgcgcg ggtcggtcgt gtagacgccg tccacgtcgg tgtagatcag
       61 gcattcgtcg gccttcatcg cggccgccac cgcgaccgcc gaggtgtccg agccgccgcg
      121 gcccagggtc gtgatatgac catcgtcgtc gatgccctgg aagcctgtga tgacgaccac
      181 cttgcctgcc tcgagatcgg cccgcacgcg cgcatcgtcg atgctctgga tgcgcgcctt
      241 ggtgtaggac gaatcggtct tcaccggcac ctgccagccg gcatagctga cggcttgcag
      301 gccttcggac tgcagcgcga tcgacagcag gccggaagag acctgctcgc cggtcgccgc
      361 gatcatgtcg agctcgcgtg ccagttcggg cgtctgcgaa ccgggcgaga gctccttggc
      421 gagcgcgagc aggcggttgg tctcgccgct catcgccgag ggcacgacca ccatctggtg
      481 gccggcgcga tgccatttgg cgacgcgctt ggcgacgttt ctgatgcgct cggtcgagcc
      541 catcgaggtg ccgccgtact tgtgaacgat caaagccatg aaaggatgcc gcgggggttg
      601 atgcgcaagg ccgctctgcg caaagagctg tcgattttag ccgccggcaa ggccgatttt
      661 gcggcgcagc aatcagcgca agtttccggc cggcttcttt gcaagccact ccagcgcgac
      721 catggcttgt cccttttccc cccaggcgcg cgcatcgatg cccagccccg gcacgaagac
      781 cagttgcccg gccacatagg ccagtggacc ctcgcgcgac caggccggca cggcggccgc
      841 ctggtattgc ttcttcaggc tgcgcgccgg ccgtcccggc tcgggctgaa actgctcgcc
      901 gccgcttcga ggcaggagcg cgagttcacc gagacgcgcc agcggcaccc cgcgttcggc
      961 gacgcgcttg acgagcaggc tgccgcccca gcccggcagc gcgtagcgtc cggcgcgacg
     1021 gatcgacaca ctggcctccc gcggctgcgt cgacgctgct tcggaaggcc gatcatgctc
     1081 gacgaagctc aggcacccgc gatagcggcg cagccagccg ccgttcaagg cccagcgcgc
     1141 cggcccgcgg ccgcgcggca gttccgcata caggcgctcc agctgggccg ccggcaaggc
     1201 ctcgccgctg cactcgcgca cccaggcgcg caaggcgttg cgcgcgcggt gggccggcag
     1261 gccggaccag gcatcgatgt ccaagccttg cggcttgacc agcttcgaaa gatccagcgc
     1321 ggccagatca ctcaggcagg cattggcctc ctgggcccag ctcgccgcat cggccagctt
     1381 ggcctcggcc tgcgggaagg ctgcactgag ctgcggccac acctcgaggc gcagccggtt
     1441 gcgggcgaag cgcgtgtcct cgttgctgtc gtcgtcaata tggttcagcc tgtgcaggcg
     1501 cacataggcc tcgatcgcct cgcgtggcag cttgagccaa ggccgcaacc aggcaatgcc
     1561 cccgcgttcg atggcggccg gcatgccggc cagcccggcc acgccggccc cacgcaaggc
     1621 ctgcagcagg aaggtttcgg cctggtcgcg aaggtggtgg gcgagcagca cggcttcggc
     1681 gccggccgcc agggccaggc gtcgcagcgc ggcatagcgc gcctcgcgcg cgacggcctc
     1741 cacgctgtcg ccagcccgca ggtgcaagtg caaacgctcg aaatgcaggc tcaccggcag
     1801 gccctgccgc gcccagcgcc ggcattgcgc ctcgcaatgc gcaagccagg catcggcctg
     1861 cggactgagc ccatggtgca catgcaaggc cgccacgctc aggccctgtg cctgcgccgc
     1921 ctttagcgtg gcatgcagca gcgcggtgga atctcgcccc ccgctgtagg cgatgccgac
     1981 gaccgccgtc tgacgcacct cagcaacccc tgaagcggtt gcaccgcccg cgcgacgtga
     2041 accggctgtt gccgagtggg gggcgcggat ccggctttgc cgggccgccg acgccgcccc
     2101 cttgaggggg tgcgccgaag gcgctccggg ggggagccat cagctgtctt tggtatcggt
     2161 gtagcgcccg tagctttgca ggcgcttgta gcgctgatcc agcagctctc gcg
//
LOCUS       DASUCY010000146         3246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_51367, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000146 DASUCY010000000
VERSION     DASUCY010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51367"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..756
                     /locus_tag="VFV25_02860"
     CDS             157..756
                     /locus_tag="VFV25_02860"
                     /inference="COORDINATES: protein motif:HMM:NF017269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEY1392285.1"
                     /translation="MNARKKLFPAKLRAARWLVLAPFVVLTLSACVVAPPPRPYYATA
                     PAPPPPPARTTLFFYPQQQQPQAQQERDRYECYRWAVSQTGVDPGMTPLSASPPVPPP
                     PPVYNDGRNTVGGAVVGGVVGASVAPPWRSGEGFVIGALLGGIVGAAADNARTEAIQQ
                     AREARYQAAQARAAQANAPYEAFRGAMTNCMTSRGYTVQ"
     gene            779..1456
                     /locus_tag="VFV25_02865"
     CDS             779..1456
                     /locus_tag="VFV25_02865"
                     /inference="COORDINATES: protein motif:HMM:NF040452.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6515 family protein"
                     /protein_id="HEY1392286.1"
                     /translation="MSLPARALVLGLVALMPALAVLPARADNDRHGNAHGMAAPYARP
                     GEWYDNRWGHSHYYPAPGRAVPVLPGGAQLAVRGGSRYWYSGGVWYGPGPRGYVVVSP
                     PFGVYVDVLPAFATAVVLGGITYYYANDVYYQPVPTGGYQVVAPPAQGEVSAAPQQLY
                     VYPRQGQGAQQQAKDEYECHQWAVSKTGFDPTVAASGGDAGTPSQRADYARADTACLE
                     GRGYTVR"
     gene            complement(1479..2909)
                     /gene="pyk"
                     /locus_tag="VFV25_02870"
     CDS             complement(1479..2909)
                     /gene="pyk"
                     /locus_tag="VFV25_02870"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEY1392287.1"
                     /translation="MPRATKIVATLGPASSTPEILERMIRAGVDVVRLNFSHGKAQDH
                     IDRAALVREAAQRAGKEVAIMADLQGPKIRVGKFAEGRVMLEPGQPFVLDAARSEPGD
                     LSGVGLDYKELPRDVKPGDTLLLNDGLIVLTVDAVKSDAVHTIVKLGGELSNNKGINK
                     QGGGLTAPALTAKDMEDIKTAMSFQCEYLAVSFPKSATDMEMARQLAYVAGEPWRHKP
                     ALIAKIERSEAIPLLDQILKASDGIMVARGDLAVEVGNAAVPALQKRMIRLAREMDKV
                     VITATQMMESMIVNPVPTRAEVSDVANAVLDGTDAVMLSAETAAGKYPLETVEQMAAI
                     AQEAEWAEEIELDADFINKHFGRIDQSIAMGALFTAHHLGCKAIVALTESGSTALWMS
                     RHKIHVPIYGLTSQLASQRRMTLYRNVVPLLMPAHNDRDVALKEAERLLVERGVLKPG
                     DTYAITCGEPMGYPGGTNMLKVCRVA"
     gene            complement(2959..>3246)
                     /gene="pgk"
                     /locus_tag="VFV25_02875"
     CDS             complement(2959..>3246)
                     /gene="pgk"
                     /locus_tag="VFV25_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEY1392288.1"
                     /translation="QLAEQLKAAGTIVWNGPVGVFEFDAFAHGTETIARAVAASSAFS
                     IAGGGDTLAAIAKYGIEKEVGYISTGGGAFLEVLEGKTLPAFEILQRRATA"
BASE COUNT          457 a         1133 c         1104 g          552 t
ORIGIN      
        1 gcggctttgc ggctgcctac gcgcgctgcg ggaatgctcg gggctcggcc gagaagcgcc
       61 ctcctaaact ggctgctgtt ggggtggccg aacaatgctt aacaaagttt cacccacaga
      121 cgcaggacac ttgcacagca agccgaacat gcacgcatga acgctcgcaa gaaattgttc
      181 cccgccaagc tgcgcgccgc gcgctggctg gtgctcgcac cctttgtggt gctgacgctg
      241 agtgcctgcg tggtggcgcc gcctccgcgg ccctactacg cgacggcacc ggcgccgcca
      301 ccgccgccgg ctcgcacgac actcttcttc tatccgcagc aacagcagcc ccaggctcag
      361 caggagcgcg accgctacga gtgctatcgc tgggctgtga gtcagaccgg cgtcgatccg
      421 ggtatgacgc cgctcagcgc ctcgccgccc gtgcctccgc ccccgccggt ctacaacgac
      481 gggcgcaaca cggtgggcgg ggccgtggtg ggcggcgtgg tcggcgcctc ggtggcgccg
      541 ccctggcgca gcggcgaagg cttcgtgatc ggtgcgctgc tgggcggcat cgtcggtgcg
      601 gcggccgaca acgcgcgcac cgaggcgatc cagcaggccc gcgaggcgcg ctaccaggcc
      661 gcgcaggccc gggctgccca ggccaatgca ccctacgagg ctttccgagg cgcgatgacg
      721 aattgcatga cgtcgcgcgg ctacacggtg cagtgaggcc gccgaaagga tgaacgccat
      781 gtctcttcct gcccgcgcgc tggtgctcgg cctcgtcgcg ttgatgccgg ccctcgcggt
      841 gctacctgcc cgcgccgaca acgatcgcca cggcaatgcg cacggcatgg ccgcgccgta
      901 cgctcggccg ggcgagtggt acgacaaccg ctggggtcat agtcactact accccgcgcc
      961 cggccgtgcc gtgccggtcc tgccgggcgg cgcccagctc gcggtgcgcg gcggctcgcg
     1021 ctactggtac tcgggcggcg tctggtacgg ccccggcccg cgcggctatg tcgtggtgag
     1081 cccgcccttc ggcgtctacg tggacgtgct gccggccttc gccactgcgg tcgtgctggg
     1141 cggcatcacc tactactacg ccaacgacgt ctactaccag ccggtgccca ccggcggcta
     1201 ccaggtcgtc gccccgcccg cgcagggcga agtgtcggcc gcaccccagc agctctacgt
     1261 ctatccgcgc caaggccagg gcgcgcagca gcaggcgaag gacgagtacg agtgccacca
     1321 atgggcggtg tccaagaccg gcttcgaccc gacggtggcg gccagcggcg gcgatgcagg
     1381 gacgcccagc cagcgggccg attacgcccg cgccgatacc gcctgtctgg aaggccgcgg
     1441 ctataccgtc cgctagcagc tagcgctccg ggggtgggtc aagccacccg gcagaccttg
     1501 agcatgttgg tgccgcccgg atagcccatg ggctcgccgc aggtgatggc ataggtgtcg
     1561 ccgggcttga gcacgccgcg ctcgacgagc aggcgctcgg cctccttgag cgccacgtcg
     1621 cggtcgttgt gggccggcat cagcagcggc acgacattgc gatacagggt catcctgcgc
     1681 tgcgaagcga gctgagaggt cagcccgtag atcggcacat ggatcttgtg acggctcatc
     1741 cacagtgcgg tggagcctga ctcggtcagc gccacgatcg ccttgcagcc caggtggtga
     1801 gccgtgaaca gcgcgcccat cgcgatgctc tggtcgatac ggccgaagtg cttgttgatg
     1861 aagtcggcat ccagctcgat ctcctcggcc cactcggctt cctgcgcgat ggccgccatc
     1921 tgctctaccg tctccagcgg gtatttgccg gcagccgtct cggcgctcag catcacggca
     1981 tcggtgccgt cgagcacggc attggccacg tcgctgacct cggcgcgcgt gggcaccggg
     2041 ttgacgatca tcgactccat catctgggtc gccgtgatca cgaccttgtc catttcgcgg
     2101 gccagcctga tcatgcgctt ttgcagcgcg ggcacggccg cgttgcccac ttcgaccgcg
     2161 agatcgccgc gtgccaccat gatgccgtcg ctggccttga ggatctggtc cagcagcggg
     2221 atggcttcgc tgcgctcgat cttggcgatc agcgcgggct tgtggcgcca gggctcgccc
     2281 gcgacatagg cgagctggcg cgccatctcc atgtcggtcg cgctcttggg gaagctcacc
     2341 gccaggtatt cgcactggaa gctcattgcg gtcttgatgt cttccatgtc cttggcggtg
     2401 agggccggcg ccgtgagccc gccgccctgc ttgttgatgc ccttgttgtt ggacagctcg
     2461 ccgccgagct tgacgatggt gtgcacggca tcgctcttca cggcgtccac cgtcaacacg
     2521 atcaggccgt cgttgagcag cagcgtgtcg ccgggcttca cgtcgcgcgg cagctccttg
     2581 tagtccaggc ccacgccgct caggtcgccg ggctcgctgc gtgccgcatc cagcacgaag
     2641 ggctggccgg gctccagcat caccctgcct tcggcgaact tgccgacgcg gatcttgggc
     2701 ccctgcaggt cggccatgat cgcgacttcc ttgccggccc gctgggccgc ctcgcgcacc
     2761 agtgccgcgc ggtcgatgtg gtcctgggcc ttaccgtgcg agaagttgag ccgcaccacg
     2821 tcgaccccgg cgcggatcat gcgctcgagg atctcaggcg tggaagaggc ggggccgagg
     2881 gtggcgacga tcttggtggc gcggggcatg gtcttcctgt tgtctccgtg attctttgaa
     2941 tggctgcggc cgaaggccct aggccgtcgc gcgccgctgc aggatttcga aggccggcag
     3001 cgtcttgccc tccagcacct cgaggaaggc gccgcctccg gtagagatgt agcccacctc
     3061 cttctcgatg ccgtacttgg cgatcgcggc cagcgtgtcg ccgccgccgg cgatgctgaa
     3121 ggcgctcgac gcggcgacgg cgcgcgcgat ggtctcggtg ccatgggcga aggcgtcgaa
     3181 ctcgaacacg cccaccgggc cgttccacac gatggtgccg gccgccttga gctgctcggc
     3241 cagctg
//
LOCUS       DASUCY010000147         3017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_372802, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000147 DASUCY010000000
VERSION     DASUCY010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372802"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..1585
                     /locus_tag="VFV25_02880"
     CDS             5..1585
                     /locus_tag="VFV25_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY1392289.1"
                     /translation="MAATLTACAAAFTAFGAQAASTLIYCSEGSPEGFDPARFTAGTT
                     FNASSQPIFNRLVEFEIGGTRVEPGLAERWEISPDGLNYTFHLRHGVKFHTTEWFKPS
                     RDFDADDVVFSFDRMINKDNAFTKAAPASFEYANDMGLPADIVRIEKLDPYTVRFVLK
                     NVDAAFLADLAMDFASVQSAEYAAQLLKAGQAGELNNKPVGTGPFVFKRYEKDAQIRY
                     AAFDQYWRGKAKIDNLIYAITKDSAVRVQKLKAGECQVSIYPKPAEVEVLKKEPNVKL
                     QSQPGLNVGYLALNVTHKPFDKLEVRQAINMAINKKALLDAVYQGAGTIAVNPIPPTM
                     WSWNKQIKDYEYNPEKAKALLAKAGYPNGFETKLWALPVQRPYNPNGQQAAELIQADL
                     AKIGIKAQIVKYEWAEYLKRAKAGESDIGMFGWNGDNGDPDNFMANLLSCSAVGGSNY
                     GQWCNKEYDALIIKAKQTSNVAERTKLYEKAQEVFKREAPWVPMAHSVVYQPMLKNVE
                     GYKMSPFASVQFWGVSIK"
     gene            1662..>3017
                     /locus_tag="VFV25_02885"
     CDS             1662..>3017
                     /locus_tag="VFV25_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY1392290.1"
                     /translation="MKSKSLTLAALSVGLSAGLFACSLARAAGSLTYCADGSPEGFDI
                     AQYETVVTNDAAIPVYEQLLAFKPGSTELVPGLAQSWDISADGREYTLHLRHAVKFHS
                     TAWFKPTREMNADDVLWSLNRINDAHHPAHAAARNGFPYWAGMSMPGLVQGIARLDDY
                     TVRITLTHPEAPFLADLAMSALASVYSAEYGEQLTRAGKLEELNTQPIGTGPYAFKSY
                     QKDAVIRYVAHNAYWGGKPALDQLVFAITPDPSVRAQRVKAGECAVANVIGEQATTLA
                     GDSRIAVVPNPQLTTSYVAPNAKRRFLSDKRFRLALSLAIDRASLVKSVYGGYATVAT
                     SFVPPGIWSHDASLANPQDIEKAKALVKASGYDGSELTLFSVARRSDNKRAVELLQAD
                     WARIGVKVRPQLMELGELYKRTGKGEHDLAFLSWFSDNGDPDNFLSPNLACAAVEGGG
                     NK"
BASE COUNT          581 a         1055 c          941 g          440 t
ORIGIN      
        1 taccctggcg gctaccctca ccgcctgcgc cgccgctttc acggctttcg gcgcccaggc
       61 cgccagcacc ctgatctatt gctccgaagg cagccccgag ggcttcgacc cggcgcgctt
      121 cacggccggc acgaccttca acgcgagctc gcagcccatc ttcaaccggc tggtggagtt
      181 cgagatcggc ggcacccgcg tcgagcccgg cttggccgag cgctgggaga tcagccccga
      241 cggcttgaac tacaccttcc atctgcgcca tggcgtgaag ttccacacca ccgagtggtt
      301 caagcccagc cgtgacttcg atgccgatga cgtggtcttc agcttcgacc gcatgatcaa
      361 caaggacaac gccttcacca aggcggcgcc ggcgtccttc gagtacgcga acgacatggg
      421 cctgccggcc gacatcgtcc gcatcgaaaa gctcgatccc tacacggtgc gcttcgtgct
      481 gaagaacgtg gacgccgcct tcctcgccga cctggcgatg gacttcgcct cggtgcagtc
      541 ggccgagtac gcggcccagc tgctgaaggc cggccaggcc ggcgagctca acaacaagcc
      601 ggtcggcacc ggccccttcg tcttcaagcg ctacgagaag gacgcgcaga tccgctacgc
      661 cgccttcgac cagtactggc gcggcaaggc caagatcgac aacctgatct acgcgatcac
      721 caaggactcc gcggtgcgcg tgcagaagct caaggccggc gaatgccagg tctcgatcta
      781 ccccaagccg gccgaggtcg aggtgctgaa gaaggagccc aacgtcaagc tgcagtcgca
      841 gcccgggctg aacgtgggct atctcgcgct caacgtcacg cacaagccct tcgacaagct
      901 ggaggtgcgc caggcgatca acatggcgat caacaagaag gcgctgctcg acgcggtcta
      961 ccagggcgcg ggcacgatcg ccgtgaaccc cattccgccg acgatgtgga gctggaacaa
     1021 gcagatcaag gactacgagt acaaccccga gaaggccaag gccctgctcg ccaaggccgg
     1081 ttatcccaac ggcttcgaga ccaagctctg ggccctgccg gtgcagcggc cctacaaccc
     1141 caacggccag caggcggccg agctgatcca ggccgacctc gccaagatcg gcatcaaggc
     1201 ccagatcgtc aagtacgaat gggccgagta cctgaagcgc gccaaggccg gcgagagcga
     1261 catcggcatg ttcggctgga atggcgacaa cggcgacccg gacaacttca tggccaacct
     1321 gctgagctgc tcggccgtgg gcggctccaa ctacggccag tggtgcaaca aggaatacga
     1381 cgcgctgatc atcaaggcca agcagaccag caatgttgcc gagcgcacca agctctacga
     1441 gaaggcgcag gaggtcttca agcgcgaggc gccctgggtg ccgatggcgc actcggtggt
     1501 ctaccagccc atgctcaaga acgtggaggg ctacaagatg agccccttcg cctcggtgca
     1561 gttctggggc gtgagcatca agtagtgact tgaacgatga agcgcggccc ccgcgcttcg
     1621 cccttgcacg actcgcacga ctcaccgtca cggatcccgc gatgaaatcg aaatcgctca
     1681 ccctggctgc cctttcggtt ggcctgtctg ccggcctgtt cgcctgcagc ctggcccgcg
     1741 ccgcgggcag cctgacctac tgcgccgacg gctcgcccga gggcttcgac atcgcgcagt
     1801 acgagactgt cgtcaccaat gacgcggcca taccggtcta cgaacagctg ctcgccttca
     1861 agcccggcag caccgagctg gtgccgggcc tggcgcagag ctgggacatt agcgccgatg
     1921 ggcgcgagta cacgctgcac ctgcgccacg ccgtgaagtt ccacagcacg gcctggttca
     1981 agcccacgcg cgagatgaac gccgacgacg tgctgtggtc gctcaaccgc atcaacgatg
     2041 cgcaccaccc ggcgcatgcg gcggcgcgca acggcttccc ctactgggcc ggcatgagca
     2101 tgcccggcct ggtccagggc atcgccaggc tggacgacta caccgtgcgc atcacgctga
     2161 cgcacccgga ggcgcccttc ctcgccgacc tggcgatgtc ggcgctggcc tcggtctatt
     2221 cggccgaata cggcgagcag ctgacgcgcg ccggcaagct ggaggagctg aacacgcagc
     2281 ccatcggcac cgggccttac gccttcaaga gctatcagaa ggacgcggtg atccgctacg
     2341 tggcccacaa cgcctactgg ggcggcaagc cggcgctgga ccagctggtg ttcgcgatca
     2401 cgcccgatcc cagcgtgcgg gcgcagcgcg tgaaggccgg tgaatgcgcg gtcgccaacg
     2461 tcatcggcga gcaggccacg accctggcgg gcgacagccg catcgcggtc gtccccaatc
     2521 cgcagttgac gacgagctat gtcgcgccca acgcgaagcg ccgcttcctg agcgacaagc
     2581 gcttccggct cgccctgtcg ctggccatcg accgcgccag cctcgtgaaa tcggtctacg
     2641 gcggctatgc aacggtggcg accagcttcg tgccgcccgg catctggagc cacgacgcca
     2701 gcctcgccaa cccgcaagac atcgaaaagg ccaaggccct ggtcaaggcc tcgggctacg
     2761 acggcagcga gctcacgctg ttctcggtcg cgcggcgcag cgacaacaag cgcgccgtgg
     2821 agctgctgca ggccgactgg gctcgcatcg gcgtcaaggt caggccgcag ctgatggagc
     2881 tgggcgagct ctacaagcgc accggcaagg gcgagcatga tctcgccttc ttgagctggt
     2941 tcagcgacaa cggcgacccc gacaacttcc tgagccccaa cctcgcctgc gcggccgtcg
     3001 aaggcggcgg caacaag
//
LOCUS       DASUCY010000148         2517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1352628, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000148 DASUCY010000000
VERSION     DASUCY010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352628"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /gene="radC"
                     /locus_tag="VFV25_02890"
     CDS             complement(<1..630)
                     /gene="radC"
                     /locus_tag="VFV25_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEY1392291.1"
                     /translation="MKDLPAELRPREKLLERGPEALADAELLALLLRTGYQGVGVLSL
                     AQRLLDEFGGITGLLRAAPEDLARIKGLGLAKRAELSAVLELSRRALATQLAERPIFE
                     SPQTVKDYLLLHLSDRAHEVFSVLFLDARHRLQAFEELFRGTLTQTSVYPREIVKRAL
                     ALNAAAVVLAHNHPSGVAEPSKADEYLTATLKSALALIDVRVLDHLVVGR"
     gene            632..1144
                     /locus_tag="VFV25_02895"
     CDS             632..1144
                     /locus_tag="VFV25_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY1392292.1"
                     /translation="MRWGRKLKSWPSLANRKQRALPTVQPGSFLTLHYRLAGPNGADL
                     VNTFSDKPATLSLGCGELAPAIEARLIGLEEGARERFELAAGEAFGVRNPELLQRVKL
                     ALLRELGDPDETYAVGDVVQFPTPDGQASYAGVVRELGPQEEDWALFDFNHPLAGQPV
                     SFEVQVIGVL"
     gene            1144..2130
                     /gene="ispH"
                     /locus_tag="VFV25_02900"
                     /gene_synonym="lytB"
     CDS             1144..2130
                     /gene="ispH"
                     /locus_tag="VFV25_02900"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEY1392293.1"
                     /translation="MTSESVLPRVDDVLLAEPRGFCAGVDRAIEIVERAILKFGAPIY
                     VRHEIVHNTYVVNELKAKGAVFIEDLAEVPPGATLVFSAHGVSKAVRVEAAQRGFSVF
                     DATCPLVTKVHVEVAKLHKEGYEFIMIGHKGHPEVEGTMGQLTEGIYLVEDVADVERV
                     QVQRPDRLAVVTQTTLSVDDAAEILAAVKRRFPQVREPKKQDICYATQNRQDAVKVLA
                     PQVDVVIVVGSPTSSNSNRLRELAERLGTPAYMIDAPEDLQPAWFAGRPRVGLTAGAS
                     APDILVQQVIARLKSLGAVSVRKMSGVAETVQFPLPKGLKSEPGDQPLQHLA"
     gene            complement(2141..2401)
                     /locus_tag="VFV25_02905"
     CDS             complement(2141..2401)
                     /locus_tag="VFV25_02905"
                     /inference="COORDINATES: protein motif:HMM:NF042692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6587 family protein"
                     /protein_id="HEY1392294.1"
                     /translation="MQSLIVALLVVGCSLYAAWTLAPKAAQRALAAGLLRLKLPAAIA
                     TKLGRVARASGGCSCDGCDAGNAAPRKPQGGPAPLRFHPRRR"
BASE COUNT          421 a          838 c          890 g          368 t
ORIGIN      
        1 gcggccgacc accaggtgat cgagcacgcg cacatcgatc agcgccagcg ccgatttcag
       61 cgtcgcggtc aggtattcat cagccttgga cggctcggcg acgccggagg gatggttgtg
      121 ggccagcacg acggcagcgg cgttgagcgc cagggcgcgc ttgacgatct cgcgcgggta
      181 gacgctggtc tgagtcagcg tgccgcgaaa cagctcctcg aaggcctgca gccggtggcg
      241 ggcatcgaga aatagcaccg agaagacttc atgggccctg tccgacagat gcagcagcag
      301 atagtccttc accgtctgcg gcgactcgaa gatggggcgc tcggccagct gggtggccag
      361 tgcgcggcgc gacagctcca gcacggccga cagctcggcg cgcttggcca gccccaggcc
      421 cttgatgcgt gcgaggtcct cgggcgcggc ccgcagcaag ccggtgatgc cgccgaattc
      481 gtccagcagc cgctgggcca ggcttagcac gcccacgccc tggtagcccg tgcgcagcag
      541 cagcgccagc agttcggcat cggccagggc ctcggggccg cgctccagca gcttctcgcg
      601 cgggcgcagt tcggcaggaa ggtctttcac gatgcggtgg ggacgaaaac taaaatcgtg
      661 gcccagtcta gcgaaccgaa agcagcgcgc cttgcctacc gtacaaccgg gttccttcct
      721 taccctgcat taccgcctgg ccgggccgaa cggcgccgat ctggtcaaca ccttcagcga
      781 caagcccgcg acgctatcgc tgggctgcgg cgagctcgcc ccggcgatcg aggcccggct
      841 gatcggcctg gaagagggcg cgcgcgagcg cttcgagctg gccgccggcg aggctttcgg
      901 cgtgcgcaac cctgagctgc tgcagcgcgt gaagctggcg cttctgcgcg agctgggcga
      961 tcccgacgag acctatgcgg tcggtgacgt cgtccagttc cccacgcccg acggccaggc
     1021 ctcctatgcc ggcgtggtgc gtgaactcgg tccgcaggag gaggattggg ccctgttcga
     1081 cttcaaccac ccgctggccg gccagccggt gagtttcgaa gtgcaagtga tcggggtgct
     1141 gtgatgacga gcgagagcgt gctgccgcgc gtcgacgatg tgctgctggc agagccgcgc
     1201 ggcttctgcg ccggcgtgga tcgagcgatc gagatcgtcg agcgggcgat cctcaagttc
     1261 ggcgcgccca tctacgtgcg ccacgagatc gtgcacaaca cctatgtggt caatgagctc
     1321 aaggccaagg gcgcagtctt catcgaggac ctggccgagg tgccgccggg cgcgacgctg
     1381 gtcttcagcg cccatggcgt gagcaaggcg gtgcgggtgg aggcggctca gcgcggcttt
     1441 tccgtcttcg acgcgacctg tccgctggtc accaaggtgc atgtcgaggt ggccaagctg
     1501 cacaaggaag gctacgagtt catcatgatc ggccacaagg gacaccccga ggtcgagggc
     1561 acgatgggcc agctcaccga gggcatctac ctggtcgagg acgtcgccga tgtcgagcgc
     1621 gtgcaggtgc agcgccccga tcgcctggcc gtcgtgacgc agaccacgct cagcgtggac
     1681 gacgcggccg agatcctggc cgccgtgaag cggcgcttcc ctcaggtgcg tgagcccaag
     1741 aagcaggaca tctgctatgc cacgcagaac cgccaggacg cggtgaaggt gctggcgccg
     1801 caggtggatg tcgtgatcgt ggtcggcagc cccaccagct ccaacagcaa ccgcctgcgc
     1861 gagctggccg agcgcctggg caccccggcc tacatgatcg atgcgcccga ggacctgcag
     1921 ccggcctggt tcgcgggccg tccgcgcgtg ggccttacgg ccggcgcctc ggcgcccgac
     1981 atcctggtgc agcaggtgat cgcgcggctg aagtcactgg gcgcggtctc ggtgcgcaag
     2041 atgagcggcg tagcggagac cgtgcaattc ccgctgccca aggggctcaa gagcgagccc
     2101 ggtgaccagc ccttgcagca cctcgcctga gcgggtacgc ctagcggcgc cgcggatgga
     2161 agcgcagcgg cgctggcccg ccctggggct tgcggggcgc agcattgccg gcatcgcagc
     2221 catcgcagga gcagccgccg ctagcgcgcg ccacgcggcc tagcttggtc gcgatcgcag
     2281 cgggcagctt caggcgcagc aagcccgcgg ccagcgcgcg ctgcgcggcc ttgggcgcga
     2341 gcgtccaggc cgcatacagg ctgcagccga cgaccagcag ggcgacgatc aggctttgca
     2401 tatcagcccc cgaaccagag cgcgacccgg taggtcacga aggacgccac ataggccagc
     2461 gcgaacagat agcccgccat gagcagcgga tagcgccagg aattcgtctc gcgcttg
//
LOCUS       DASUCY010000149         4298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_292486, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000149 DASUCY010000000
VERSION     DASUCY010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292486"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..314)
                     /locus_tag="VFV25_02910"
                     /pseudo
     CDS             complement(1..314)
                     /locus_tag="VFV25_02910"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            complement(313..372)
                     /locus_tag="VFV25_02915"
                     /pseudo
     CDS             complement(313..372)
                     /locus_tag="VFV25_02915"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(582..752)
                     /locus_tag="VFV25_02920"
                     /pseudo
     CDS             complement(582..752)
                     /locus_tag="VFV25_02920"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
     gene            complement(749..1324)
                     /locus_tag="VFV25_02925"
     CDS             complement(749..1324)
                     /locus_tag="VFV25_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1392295.1"
                     /translation="MTTVVVARKNGQLAIAADSLVTFGDTRLAHGYESNDKLFRVDDS
                     YLGLAGTVAHFPVLRKALQSLPAEDLKLHSRDQVFDTFTKLHPKLKEQFFLNPKEEDA
                     DPYESSQFTVLIANASGIYGVYSYREVFTFDRFWGIGTGRNFALGAMFAAYDKVKTAR
                     ELAEIGVRAGCEFDKNSAGPVRVHTLKLKEQ"
     gene            complement(1392..3053)
                     /gene="aceF"
                     /locus_tag="VFV25_02930"
     CDS             complement(1392..3053)
                     /gene="aceF"
                     /locus_tag="VFV25_02930"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEY1392296.1"
                     /translation="MPLVEIKVPDIGDFKDVEVIELLVKPGDAIKAEQSLITVESDKA
                     SMEIPASQGGVVKELKVKLGDKVSEGSLLLMLEANGAAVPAAAPAAASTVPAPIALAA
                     SAPAAAAPVAAPAASAGPIEVVVPDIGDFSDVAVIELLVKPGDVVKAEQSLITVESDK
                     ASMEIPSSHAGVVKSLAVKLGDKVSKGSVVAVVEATGAAPAAAAAPVAAAAAAPAPAA
                     ASVAAIPSQAAAVQPAHEPRAPTAQLPHASPSVRKFARELGVPLDEVKGSGPKGRIMQ
                     EDVQGFVKGVMAGSVQTEAQKAKAPAGGGAGGAGLDLLPWPKVDFAKFGPIERKDLSR
                     IKKISGANLHRNWVMIPHVTNNDEADITELEAFRVATNKENEKSGIKVTMLAFVIKAV
                     VSALKKFPAFNASLDGDALVYKQYYHIGFAADTPNGLVVPVLKDADKKGILQISQEMG
                     DLAKKARDGKLGPADMTGGCFSISSLGGIGGTHFTPIINAPEVAILGLSKSAMKPVWD
                     GKAFVPRLTLPLSLSYDHRVIDGAAAARFNTYLAQVLADFRRVLL"
     gene            complement(3075..>4298)
                     /gene="aceE"
                     /locus_tag="VFV25_02935"
     CDS             complement(3075..>4298)
                     /gene="aceE"
                     /locus_tag="VFV25_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576846.1"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEY1392297.1"
                     /translation="VLEPTAEGREISTTQAYVRFLTQLLRDKEFGSRVVPILVDEART
                     FGMEGLFRQIGIYNPAGQQYTPVDKDQVMYYREDKAGQILQEGINEAGGMASWIAAAT
                     SYSTSNRIMMPFYVYYSMFGFQRIGDLAWAAGDLQARGFLLGGTSGRTTLNGEGLQHE
                     DGHSHILAGTIPNCVSYDPTFAHEVGVILHHGLKRMVEKQDNVFFYLTLLNENYAMPG
                     LKSGTEEQIIKGMYLLQEGSKKTPRVNLLGSGTILRESIFAKELLEKDWGVAANVWSC
                     PSFNELARDGQDCERWNLLHPSESPRLPFVAQQLEKYAGPVIASTDYMKSYADQIRAF
                     MPKGRSYKVLGTDGFGRSDFRSKLREHFEVNRHYIVVAALKALADEGTLPAAKVAEAI
                     AKYGIKAGKINPLYA"
BASE COUNT          647 a         1424 c         1435 g          792 t
ORIGIN      
        1 gtctcttcga cgcgcagatg gtgaggatcg gcgaagctgc cgatgccgcg caccaccgtg
       61 accttgcgca tcttggccat ggcggccagg ccgccggtga gcttgcctgt gaccttgttc
      121 ttgtgcgcga gcagcttggg caagtcgagc tggggctggt catagctcac gcccaggtcg
      181 gcgaaatgct tgacctcgtc catcacggcc gccacgtgca gcagcgcctt ggacggaatg
      241 cagccgacgt tcaggcacac gccgcccagc gtcgcgtagc gttcgaccag gacggtcttc
      301 atgccgaggt cggcggcacg gaaggcggcc gaatagccgc cggggccggc gcccagcacc
      361 agcacgtcgc attcgaggtc ggcaccgccg gcatagctcg cggccgccgg tgcgacggta
      421 ggcgccgaag cgggagtcgc tacgcttttt gcaccggcat tcggcaaaga tactccgact
      481 gccggcttat tttctgcaga agctgccgcc ccttctacct ccagtgtcag gatcaccgag
      541 ccctggctca ccagatcgcc gagcttgagc ttgagctcct tgacgacgcc ggcatgcgac
      601 gacgggatct ccatcgaagc cttgtcgctt tccaccgtga tcaggctctg ctcggcctgg
      661 acggcgtcgc ccggcttgac cagcagctcg atcactgcca cgtccttgaa atcgccgatg
      721 tccggcacct tgatgtcgac gagggccatc attgctcctt tagttttagt gtgtgaaccc
      781 gcaccggccc ggcggagttc ttgtcgaatt cgcagcctgc acgcacgccg atctcggcta
      841 gctcgcgcgc agtcttgacc ttgtcatagg cggcgaacat cgcgcccagc gcgaagttgc
      901 gccccgtgcc tatgccccag aagcggtcga aggtgaagac ctcgcgatag gagtagacgc
      961 catagatgcc gctggcattg gcgatcagca ccgtgaactg gctgctctcg taggggtcgg
     1021 cgtcttcctc cttcgggttg aggaagaact gctccttgag cttgggatgc agctttgtga
     1081 aggtgtcgaa gacctggtcg cgcgaatgca gcttcaggtc ctcggccggc agcgattgca
     1141 gcgccttgcg cagcaccggg aaatgcgcca ccgtgccggc caggcccagg taggagtcat
     1201 cgacgcggaa cagcttgtcg ttgctctcgt agccgtgggc caggcgcgtg tcgccgaaag
     1261 tgacgaggct gtccgccgca atcgcgagct gcccgttctt gcgggccacg accacggtcg
     1321 tcacgccgaa ggctcctgcg tggatgcagg cgcgcgcacg gtcacccgcg cgcggcccgg
     1381 ccagcgtgct ttcacagtaa cacccggcgg aagtcggcca gcacctgcgc aaggtaggtg
     1441 ttgaagcgcg ccgccgcggc gccgtcgatg acgcgatggt cgtaggacag cgacagcggc
     1501 agcgtcaggc gcggcacgaa ggccttgccg tcccacaccg gcttcatcgc gctcttggac
     1561 agacccagga ttgccacttc gggcgcgttg atgatgggag tgaagtgcgt gccgccgatg
     1621 ccgccgagcg aggagatcga gaagcagccg cccgtcatgt cggccgggcc cagcttgccg
     1681 tcgcgcgcct tcttggcgag atcgcccatc tcctggctga tctgcaggat gcctttcttg
     1741 tcggcgtcct tgagcaccgg caccacgagg ccgttgggcg tgtcggccgc gaagccgatg
     1801 tggtagtact gcttgtagac caacgcatcg ccatcgaggc tcgcgttgaa cgcggggaac
     1861 ttcttcagtg ccgagaccac ggccttgatc acgaaggcca gcatcgtgac cttgatgccg
     1921 gacttctcgt tttccttgtt ggtcgcgact cggaaggcct cgagctcggt gatgtcggcc
     1981 tcgtcgttgt tcgtgacgtg ggggatcatc acccagttgc ggtgcaggtt ggcgcccgag
     2041 atcttcttga tgcgcgacag gtccttgcgc tcgatggggc cgaacttcgc gaagtccacc
     2101 ttgggccagg gcagcaggtc caggcctgcc ccgccggcgc caccgccggc cggcgccttg
     2161 gccttctgcg cctcggtctg cacgctgccg gccatcacgc ccttgacgaa gccctgcacg
     2221 tcctcctgca tgatgcggcc cttggggccg gagcccttga cctcatcgag cggcacgccc
     2281 agctcgcgcg cgaacttgcg caccgagggc gaggcatgcg gcagctgggc cgtgggcgcg
     2341 cgcggctcgt gagccggttg cacggcggcg gcttggctgg gtatggctgc cacggacgca
     2401 gcagccggtg ccggcgcagc cgccgcagcg gcaacgggcg ccgcagcagc cgcgggcgcc
     2461 gcgccggtgg cctcgaccac ggcgaccacc gagcccttgg acaccttgtc gcccagcttg
     2521 acggcgagcg acttgacgac gccggcatgc gacgagggga tctccatcga ggccttgtcg
     2581 ctctccaccg tgatcaggct ctgctcggcc ttgacgacgt cgccgggctt gaccagcagc
     2641 tcgatcacgg ccacgtcgga gaagtcgccg atgtccggca cgacgacctc gatcggaccc
     2701 gccgacgcgg ccggtgcagc gaccggggcc gcagcagcag gcgcgctggc ggccagcgcg
     2761 atcggcgcag gcacagtgct cgcggcggcc ggtgccgcag ccggcaccgc ggcgccgttg
     2821 gcctccagca tcagcagcag gctgccctcg ctgaccttgt cgcccagctt gaccttgagc
     2881 tccttgacca cgccgccctg cgaggcgggg atctccatcg aggccttgtc gctctccacc
     2941 gtgatcaagc tctgctcggc cttgatggcg tcgccgggct tgaccagcag ctcgatgacc
     3001 tcgacgtcct tgaagtcccc gatgtcgggc accttgattt caaccaatgg catttttctt
     3061 tgtccccgtg ggtctcaggc gtagagcggg ttgatcttgc cggccttgat gccgtacttg
     3121 gcgatcgcct cggccacctt ggccgccggc agcgtgccct cgtcagccag ggccttgagc
     3181 gcggccacca cgatgtaatg gcggttgacc tcgaaatgct cgcgcagttt gctgcggaag
     3241 tcgctgcggc cgaagccgtc ggtgcccagc accttgtagc tgcggccctt gggcatgaag
     3301 gcgcggatct ggtcggcgta gctcttcatg tagtcggtcg aggcgatcac cgggccggcg
     3361 tatttctcga gctgctgcgc gacgaagggc aggcgcggcg actccgaggg gtgcagcaga
     3421 ttccagcgct cgcagtcctg gccgtcgcgc gccagctcgt tgaagctcgg gcagctccac
     3481 acgttggcgg ccacgcccca gtccttctcc agcaactcct tggcgaagat ggattcgcgc
     3541 aggatggtgc cgctgcccag caggttgacc cgcggggtct tcttgctgcc ttcctgcagc
     3601 agatacatgc ccttgatgat ctgctcctcg gtaccggact tgaggccggg catcgcgtag
     3661 ttctcgttga gcagcgtgag gtagaagaac acgttgtcct gcttctcgac catgcgcttg
     3721 aggccgtggt gcaggatcac gccgacctcg tgcgcgaagg tcgggtcgta gctgacgcag
     3781 ttggggatgg tgccggccag gatgtggctg tggccgtcct cgtgctgcag gccctcgccg
     3841 ttgagcgtgg tgcgccccga ggtgccgccc agcaggaagc cacgcgcctg caggtcgcct
     3901 gcggcccagg ccaggtcgcc gatgcgctgg aagccgaaca tcgagtagta cacgtagaac
     3961 ggcatcatga tgcggttgct cgtgctgtag ctcgtcgccg cggcgatcca gctcgccatg
     4021 ccgccggcct cgttgatgcc ttcttgcagg atctgcccgg ccttgtcctc gcggtagtac
     4081 atgacctggt ccttgtcgac cggggtgtac tgctggcccg ccgggttgta gatgccgatc
     4141 tggcggaaca ggccctccat gccgaaggtg cgcgcctcgt cgaccaggat gggcaccacg
     4201 cgcgagccaa attccttgtc gcgcagcagc tgggtcagga agcgcacata ggcctgggtg
     4261 gtggagatct cgcggccttc ggcagtgggc tccagcac
//
LOCUS       DASUCY010000150         5859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_677932, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000150 DASUCY010000000
VERSION     DASUCY010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 5859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5859
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677932"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="VFV25_02940"
     CDS             complement(<1..913)
                     /locus_tag="VFV25_02940"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDR/VanB family oxidoreductase"
                     /protein_id="HEY1392298.1"
                     /translation="MITVRVEAKEPAAQGVCALTLVAADGGALPAFTAGAHLDVHLPG
                     GLVRQYSISNDPAETHRYVLGVLLDTQSRGGSRAVHEQLAPGQTLQVSAPKNHFPLAV
                     HTRRSLLIAGGIGITPILAMARQLASEDADFEFHYAASCAERMAFRAEIEASAWAEVA
                     HFHVGHDRLDVERLLAAQDAGVHVYVCGPKRLIDAVSDAARRQGWAGERIHHEFFAGA
                     EPSKPGQNSAFEIELAGSGRIIPVAADQTAAQALIAAGVSLLTSCEQGVCGTCLTRVL
                     AGEPEHRDLYLTPEEQAANDQFTPCCSR"
     gene            complement(910..1938)
                     /locus_tag="VFV25_02945"
     CDS             complement(910..1938)
                     /locus_tag="VFV25_02945"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY1392299.1"
                     /translation="MFITNTWYVAARSDEVGADKPLGRKVCNHAMAFFRNSAGRVSAV
                     EDFCPHRGAPLSLGRVHGDTLVCGYHGLAMGCDGKTVSMPCQRVRGFPAIRSFPVHEA
                     HGFIWVWPGDAAQADVAKLPAFEWLGNPAFGYGGGLYHIACDYRLMIDNLMDLTHETY
                     VHAGSIGQKEIDEIPCTTRVDGDHVITERYMQAIEAPPFWKMALRMNGLPDDQPVDRW
                     QICHFTPPSHIMIEVGVALAGQGGYEAPDAVKAASWVVDFITPETDTSIHYFWGMARK
                     FKPQDEVLTAQIREGQGKIFAEDQEMLERQQLNLLAWPQRSLLMLNIDGGGVQSRKVI
                     ERVRAAQA"
     gene            complement(1959..2345)
                     /locus_tag="VFV25_02950"
     CDS             complement(1959..2345)
                     /locus_tag="VFV25_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392300.1"
                     /translation="MNRHALSVRRRLLLGFGLQIVLLTALAAGTVWQLRSMGLQIERI
                     VEGHDRRSDLAHRLNAAQLDWREQLRALLTVSDPEDQKAQCAELIAAQRRHVQGRAEE
                     LQTAMGGFHLGGAVLATSGLPPPPSC"
     gene            complement(2348..3145)
                     /locus_tag="VFV25_02955"
     CDS             complement(2348..3145)
                     /locus_tag="VFV25_02955"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEY1392301.1"
                     /translation="MPKPNRRFVDLSIYLENDVLSDPPPLAPKITYQKHTDTVKEFMA
                     LLPGTQAEDYPDGEAAAAEWVTLTTHNGTHLDAPYHFHSTMDAKLGGSRPSTTIDEVP
                     LEWCFQPGVKLDFRHFPDGYVVTAADVQAELARIDYGLQPLDIVMVNTRAGSRYGHND
                     YVNAGCGMGYEATMYLLERGVRLTGTDAWSWDAPFTYTAKKVAETGDKSLIWEGHKAG
                     RDIGYCHLEKLHNLEALPPHGFTVSCFPHKIRGASAGWTRAVAIFES"
     gene            complement(3160..4149)
                     /locus_tag="VFV25_02960"
     CDS             complement(3160..4149)
                     /locus_tag="VFV25_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006399574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY1392302.1"
                     /translation="MKFATLRDGSPDGRLIVVSRDLSRAVDAAALAPSLLHALRHWRD
                     VQAPLQALYERLNAGTVPGSFGFDATCCAAPLPRSPQWLDGSAFLNHGRLMERAFNTK
                     PIPKFDTVPVMYQGASDDFLGPHDDVPLPSEVDGIDFEGEFGVVVDGVPMGATPAQAL
                     AAVRLVVQINDWSLRALGPHEMATGFGFLQAKPSTSFAPVAVTPDELGDAWHDGRVQM
                     TLHVALNGQRFGEPNGREMNFGFGQLVAHAARTRRLSPGAIVGSGTVSNESRAAGSAC
                     IAERRVIEIIDQGEARTPFMRFGDRVRMQARFADGRPGPFGIIDQPVVPAPAN"
     gene            complement(4169..5311)
                     /locus_tag="VFV25_02965"
     CDS             complement(4169..5311)
                     /locus_tag="VFV25_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1392303.1"
                     /translation="MVKTESVLGRGVLMAAHCAGMIDLVTLPVWVGTLISSYRFDAQQ
                     AGALVTLFLAGAVVASLFFAPRFHRLRGRMAAPLGYGLAALAFVLVSLTQDFALMAAL
                     HALAGLATGCGLSFTHGTIGRSANPHRLFALAGLALGVFAIAMLGGLPALVAAVGGAA
                     MFRVFALVMAVAAVLSALAFPTPHEAQAEAAGPGGRLSAAAWLVMAGVSCMALVQAML
                     FSFVERIGAERFGIQAVTSMLIALGFVTLLPAPLAALLQKSLPPHRVLLAGPVAQALV
                     AVGIAQSLLFAPYAVAAILLPCVMIFTHTFAFGALARLDLTGRAVAATPAMTMVGAAV
                     GPFLGGTLVKFGGYSPLALAACAVATVAIGCFAAMPRFASPLPATP"
     gene            complement(5335..>5859)
                     /locus_tag="VFV25_02970"
     CDS             complement(5335..>5859)
                     /locus_tag="VFV25_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392304.1"
                     /translation="ESTPPAPALSYGAHKLMAEIQLADLSRRDLLDGLSLRLPGIVAR
                     PPSTTGHGSAFMSELIRRLRAGEPYDCPVSAVARCWWMSRRCCVSNLLHAAGIDTQRL
                     PPQRVVQLPVIAATVHEVAMAAADQAPQVRYQPDERLEAVFGRMPALHAPQARELGFV
                     DDGSLDNLVNNAPA"
BASE COUNT          921 a         1921 c         2129 g          888 t
ORIGIN      
        1 agcgggagca gcagggcgtg aactggtcgt tcgcggcttg ctcctccggc gtcaggtaga
       61 gatcacgatg ctcgggttcg ccagccagca cgcgcgtcag gcaagtgccg cagacgcctt
      121 gttcgcagga ggtgagcagc gagacgccgg cagcgatgag ggcttgggca gcggtctggt
      181 cggcggccac cgggatgata cggccggaac ccgcgagttc gatctcgaag gcgctgttct
      241 ggcccggctt ggacggctcg gcaccggcga agaactcgtg gtggatgcgc tcaccagccc
      301 agccctggcg cctcgccgcg tcggacaccg cgtcgatcag acgcttgggc ccgcagacat
      361 agacatgcac gccggcgtcc tgcgcggcca gcagccgttc cacgtccagg cggtcgtggc
      421 cgacgtggaa atgtgccacc tcggcccagg cgctcgcctc gatctcggcg cggaaggcca
      481 tgcgctccgc gcagctcgcc gcgtagtgga actcgaaatc ggcgtcttcg ctggcgagct
      541 ggcgggccat cgcgaggatg ggcgtgatgc cgatgccgcc ggcgatcagc aggctgcggc
      601 gggtatggac cgccagcggg aagtgattct tgggtgcgct gacttgcagc gtctgccctg
      661 gcgctagctg ctcgtgcacc gcccgcgagc cgccacgcga ttgggtatcg agcagcacgc
      721 cgagcacata gcgatgtgtc tcggcgggat cgttgctgat cgagtattgc cgcacgaggc
      781 cgccgggcag gtggacatca aggtgtgcac cagccgtgaa ggcgggcagg gcgccgccat
      841 cggcggccac gagggtgagg gcgcagacgc cctgcgcggc cggctccttg gcctcgacgc
      901 ggacggtgat catgcctgcg ccgccctcac gcgctcgatg accttgcgcg actgcacgcc
      961 gccgccgtcg atgttgagca tcagcagcga gcgctgcggc caggccagca gattgagctg
     1021 ctggcgctcc agcatctcct ggtcctcggc aaagatcttg ccctggccct cgcggatctg
     1081 cgccgtcagc acttcgtcct gcggcttgaa cttgcgcgcc atgccccaga agtagtggat
     1141 ggacgtgtcg gtctcggggg tgatgaagtc caccacccag ctcgccgcct tgactgcgtc
     1201 gggggcttcg tagccgccct ggccggctag cgccacgccg acctcgatca tgatgtggct
     1261 gggcggcgtg aagtggcaga tctgccagcg gtccaccggc tggtcgtcgg gcaggccgtt
     1321 catgcgcagg gccatcttcc agaacggcgg cgcctcgatg gcctgcatgt agcgttcggt
     1381 gatgacgtgg tcgccatcga cgcgggtcgt gcaggggatt tcgtcgatct ccttctggcc
     1441 gatgctgcca gcgtgcacgt aggtctcgtg cgtgaggtcc atgaggttgt cgatcatcag
     1501 ccggtagtcg caggcgatgt ggtacagccc gccgccgtag ccgaaggcgg ggttgcccag
     1561 ccattcgaag gccggcagct tggccacgtc ggcctgggcg gcgtcgccag gccagaccca
     1621 gatgaagcca tgcgcctcat ggacagggaa gctgcggatg gcggggaagc cgcgcacgcg
     1681 ctggcagggc atggagacgg tcttgccatc gcaacccatg gctaggccgt ggtagccgca
     1741 gaccagggtg tcgccgtgta cgcgccccag cgacagcggt gcgccgcggt gggggcagaa
     1801 gtcctctacc gcgctgaccc ggccggcgct gttgcggaag aaggccatcg cgtgattgca
     1861 gaccttgcga cccaggggct tgtcggcgcc gacctcgtcg ctgcgggcgg ccacgtacca
     1921 ggtgttggtg atgaacatgg ggacagatcc ttggtgaatt agcacgacgg cgggggtggc
     1981 aggccgctgg tcgccaggac ggcgccgcca agatggaaac cgcccatggc ggtctggagc
     2041 tcttccgcac ggccctgcac atggcgccgc tgcgcggcga tcagctcggc gcactgggcc
     2101 ttctgatcct cgggatcgct gaccgtcaac agcgcgcgca gctgttccct ccaatcgagc
     2161 tgggccgcgt tgagccgatg cgcgaggtcg ctgcggcggt cgtggccttc gacgatgcgt
     2221 tcgatctgca aacccatcga gcgaagctgc cacaccgtgc ccgcagcgag agctgtcagc
     2281 agcacgatct gcaggccaaa gcccagcagc agccgcctgc gcacgctgag tgcatgacgg
     2341 ttcacgatca gctctcgaaa atcgcgaccg cccgcgtcca cccggccgag gcgccgcgga
     2401 tcttgtgcgg gaagcagctc acggtgaagc cgtggggcgg cagcgcctct aggttgtgca
     2461 gcttctcgag gtggcagtag ccgatgtcgc ggccggcctt gtggccctcc cagatcagcg
     2521 acttgtcacc cgtctcggcg accttctttg ccgtgtacgt gaaaggcgcg tcccagctcc
     2581 aggcgtcggt gccggtcagc cgcacgccgc gttccagcag gtacatggtg gcctcatagc
     2641 ccatgccgca gccggcgttc acgtagtcgt tgtgcccgta gcgggagccc gcccgcgtgt
     2701 tgaccatcac gatgtccagc ggctgcagtc cgtagtcgat gcgcgcaagc tccgcctgca
     2761 cgtcggccgc ggtgacgacg tagccgtcgg ggaagtggcg gaagtccagc ttcacgccgg
     2821 gctggaagca ccactccagc ggtacttcgt cgatggtggt ggacggccgc gacccgccga
     2881 gcttggcgtc catcgtcgaa tggaagtgat aaggcgcgtc gaggtgagtg ccgttgtgcg
     2941 tggtcaacgt cacccactcg gccgcggcag cctcgccgtc ggggtagtcc tcggcctggg
     3001 tgcccggcag cagcgccatg aactccttca ccgtgtcagt gtgcttctgg taggtgatct
     3061 tgggcgccag cggcggcggg tcggacagca cgtcgttctc gaggtagatc gacaggtcaa
     3121 cgaagcggcg attgggtttg ggcatgggaa agctctgttt cagttcgcag gggccggcac
     3181 caccggctgg tcgatgattc cgaaggggcc ggggcggcca tcggcaaagc gggcctgcat
     3241 gcgcacgcgg tcgccgaaac gcatgaatgg cgtgcgggct tcgccctgat caatgatctc
     3301 gatcacgcgg cgttcggcga tgcaggccga gccggcggcg cggctttcgt tggacacggt
     3361 gcccgagccg acgatggcgc cgggcgacag ccgccgggtg cgcgccgcgt gcgcgaccaa
     3421 ctgcccgaag ccgaagttca tctcgcggcc gttgggctcg ccgaagcgct gcccgttcag
     3481 cgctacgtgc aacgtcatct gtacgcggcc gtcgtgccag gcgtcgccca gttcatcggg
     3541 cgtcacggcc actggcgcaa agctggtcga cggcttggcc tgcaggaagc cgaagcccgt
     3601 ggccatctcg tgcgggccca gggcgcgtag gctccagtcg ttgatctgca cgacgaggcg
     3661 cacggcggca agcgcctgcg cgggcgtggc gcccatgggc acgccgtcga cgaccacgcc
     3721 gaactcgccc tcgaagtcga tgccgtcgac ctcgctgggc agtggcacgt cgtcatgggg
     3781 gccgaggaag tcgtcgctgg cgccttggta catcacgggc acggtgtcga acttcgggat
     3841 cggcttggtg ttgaaggcgc gctccatcag gcggccatgg ttcaggaagg ccgagccgtc
     3901 cagccattgc gggctgcgtg gcagcggggc ggcgcagcag gtggcgtcga agccgaagct
     3961 gcccggcacg gtaccggcgt tcaggcgttc gtacaaggcc tgcagcggtg cttgcacgtc
     4021 ccgccagtgg cgcagcgcat gcagcaggct tggggccagg gcggcagcgt cgacggcgcg
     4081 cgagaggtcg cgcgaaacca cgatcaaccg gccgtccggc gatccgtcgc gcagcgtggc
     4141 gaatttcatg ggagtccttt tcgagggttc agggcgtggc gggcaggggg ctcgcgaagc
     4201 gcggcatggc ggcgaagcag ccaattgcta cggtggcgac ggcgcaggcg gccagcgcca
     4261 aggggctgta gccgccgaac ttgacgagcg tgccgccgag gaaggggccg accgccgcgc
     4321 cgaccatggt catcgccggc gtggcggcca cggcgcggcc ggtgaggtcc agccgcgcga
     4381 gcgcgccgaa cgcgaaggtg tgggtgaaga tcatcacgca gggcagcagg atggctgcga
     4441 ccgcgtaggg cgcgaacagc aggctctgcg cgatgccgac tgccaccaag gcttgcgcga
     4501 ccggccccgc gagcagtacg cgatgcggcg gcaggctctt ttgcagcagg gcggccagcg
     4561 gcgcgggcaa cagcgtgacg aagcccagcg cgatgagcat cgacgtgacc gcctggatgc
     4621 cgaagcgctc cgcgccgatg cgctcgacga agctgaagag catggcctgc accagcgcca
     4681 tgcagctgac gcctgccatg acgagccagg ccgccgcaga cagccgcccg cccggcccgg
     4741 ccgcttccgc ctgggcctca tgcggtgtcg gaaacgccag ggccgacaac acggctgcga
     4801 cggccatcac cagcgcaaag acgcggaaca tcgccgcccc gcccacggcg gcgaccaggg
     4861 ccggcaggcc gcccagcatc gcgattgcaa acacccccag cgccaggccg gccagcgcga
     4921 acagccgatg cgggttggcg ctgcggccga tggtgccatg ggtgaaggac agcccgcagc
     4981 ccgtggcgag gccggccagc gcgtgcagcg ccgccatcag tgcgaagtcc tgcgtcagcg
     5041 acacgagcac gaaggccagc gccgcgaggc cgtagcccag cggcgccgcc atgcggcctc
     5101 gcaggcggtg aaagcgcggc gcgaagaaca agctggccac cacagcgccg gccaggaaca
     5161 gcgtgaccaa cgcacccgcc tgctgcgcat cgaagcggta gctggagatc agcgtgccca
     5221 cccacacggg cagggtgacc aggtcgatca tgccggcgca gtgcgctgcc atcagcacgc
     5281 cgcgtccgag gacgctttcg gtcttgacca ttggaattcc tttcatggcc agtgtcatgc
     5341 cggcgcgttg ttgaccaggt tgtcgaggct gccgtcatcg acgaagccga gttcacgggc
     5401 ctgtggcgca tgcagcgcgg gcatgcggcc gaagaccgct tccaggcgtt cgtcgggctg
     5461 gtagcgcacc tggggtgcct ggtcggcggc ggccatggcg acttcatgca ccgtggccgc
     5521 aatcacgggc agttgcacta cacgctgggg cggcagccgc tgcgtgtcga tgcccgcggc
     5581 gtgcagcagg ttgctcacgc agcagcgccg cgacatccac cagcagcgcg ctacggccga
     5641 cacggggcag tcgtagggct cgccggcgcg aaggcggcgg atcagttcgc tcatgaaggc
     5701 cgagccatgg ccggtggtcg agggcggccg cgcgacgatg cccggcaggc gcagcgacaa
     5761 gccatccagc aggtcgcggc ggctgaggtc ggcgagctgg atttctgcca tcagcttgtg
     5821 cgcgccgtag ctgagcgccg gcgcgggcgg ggtcgattc
//
LOCUS       DASUCY010000151         2511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_180165, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000151 DASUCY010000000
VERSION     DASUCY010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180165"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2511
                     /gene="rpoC"
                     /locus_tag="VFV25_02975"
     CDS             <1..>2511
                     /gene="rpoC"
                     /locus_tag="VFV25_02975"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEY1392305.1"
                     /translation="EKRMLQEAVDSLLDNGRRGKAMTGANKRALKSLADMIKGKSGRF
                     RQNLLGKRVDYSGRSVIVVGPTLKLHQCGLPKLMALELFKPFIFSRLEAMGIATTIKA
                     AKKEVEAGTPVVWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLHPLVC
                     AAFNADFDGDQMAVHVPLSIEAQMEARALMLASNNVLFPANGEPSIVPSQDVVLGLYY
                     ATRERTNAKGEGIIFSDVAEVQRALDNGQVEITAKISVRLTEWTRDKDSGHFVPQVKL
                     VDTTVGRALLSEILPKGLPFSNLNKTLKKKEISRLINVSFRKCGLKETVVLADKLLQS
                     GFRLATRAGISIGIDDMLVPKEKHGIIERAEKEVKEIEQQYVSGLVTAGERYNKVVDI
                     WGKAGDEVGKVMMSQLAKQKVIDRHGKEVDQESFNSIYMMADSGARGSAAQIRQLAGM
                     RGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTRRLV
                     DVTQDLVVTEDDCGTDHGIAMRALVEGGEVIESLRDRILGRVAAIDVLHPETQEVIIP
                     AGQMLDEEALDLIEQAGADEIKVRTPLTCQTRYGLCAKCYGRDLGRGGLVNSGEAVGV
                     IAAQSIGEPGTQLTMRTFHIGGAASRAAVASSVEAKSDGIIGFNATMRYVTNGKGELV
                     VISRSGEIIISDQHGRERERHKVPYGATLNIKADQHIKAGLVLANWDPLTRPIITEFA
                     GRAKFENVEEGVTVAKQVDEVTGLSTLVVIDPKRRGAAKVVRPQVKLLDAGGNEVKIP
                     GTDHSVTIGFQVGALIQVRDGQDLAPGEVLARIPVEGQK"
BASE COUNT          480 a          829 c          845 g          357 t
ORIGIN      
        1 gagaagcgca tgctgcaaga ggctgtcgac tcgctgctgg acaacggccg ccgcggcaag
       61 gcgatgacgg gcgccaacaa gcgcgcgctg aagtcgctgg ccgacatgat caagggcaag
      121 agcggccgct tccgccagaa cctgctgggc aagcgcgtcg actactcggg ccgctcggtc
      181 atcgtggtgg gccccacgct caagctgcac cagtgcggcc tgcccaagct gatggcgctg
      241 gagctgttca agcccttcat cttctcgcgt ctggaggcga tgggcatcgc caccacgatc
      301 aaggcggcca agaaggaagt ggaagccggt acgccggtgg tgtgggacat ccttgaagaa
      361 gtcatcaagg agcacccggt gctgctgaac cgcgcgccga cgctgcaccg cctgggcatc
      421 caggccttcg agccggtgct gatcgagggc aaggccatcc agctgcaccc gctggtctgc
      481 gcggccttca acgcggactt cgacggcgac cagatggccg tgcacgtgcc gctgtcgatc
      541 gaagcgcaga tggaagcccg cgcgctgatg ctggcctcca acaacgtgct gttcccggca
      601 aacggcgagc cctccatcgt gccctcgcag gacgtggtgc tgggcctgta ctacgccacc
      661 cgcgagcgca ccaacgccaa gggcgaaggc atcatcttct cggacgtcgc cgaagtgcag
      721 cgcgcgctgg acaacggcca ggtcgagatc actgccaaga tcagcgtgcg tctgaccgag
      781 tggaccaggg acaaggacag cggccatttc gtgccccagg tcaagctggt cgacacgacc
      841 gtgggccgcg ctctgctgag cgagatcctg cccaagggcc tgcccttcag caacctgaac
      901 aagacgctga agaaaaagga gatctcgcgc ctgatcaacg tttccttccg caagtgcggc
      961 ctcaaggaga cggtggtgct ggccgacaag ctgctgcaaa gcggcttccg cctggccacg
     1021 cgcgccggca tctcgatcgg catcgacgac atgctggtgc cgaaggagaa gcacggcatc
     1081 atcgagcgcg ccgagaagga agtgaaggag atcgagcagc agtacgtctc gggtctcgtc
     1141 acggcgggcg agcgctacaa caaggtggtg gacatctggg gcaaggccgg cgacgaggtc
     1201 ggcaaggtca tgatgagcca gctcgccaag cagaaggtca tcgatcgcca cggcaaggaa
     1261 gtggaccagg agtccttcaa ctccatctac atgatggccg actcgggcgc gcgcggctcc
     1321 gcggcgcaga tccgccagct ggccggcatg cgcggcctga tggccaagcc cgatggctcc
     1381 atcatcgaga cgccgatcac cgcgaacttc cgcgagggcc tgaacgttct gcagtacttc
     1441 atctccaccc acggcgcccg caagggcctg gcggacacgg cgctgaagac cgcgaactcc
     1501 ggctacctga cgcgccgcct ggtggacgtg acacaggacc tggtcgtgac cgaagacgac
     1561 tgcggcaccg accatggcat cgcgatgcgc gcgctggtcg aaggcggcga ggtcatcgag
     1621 tcgctgcgcg accgcatcct gggccgcgtc gcggccatcg acgtgctgca tcccgagacc
     1681 caggaagtca tcatcccggc cggccagatg ctggacgagg aagcgctgga cctgatcgag
     1741 caggccggcg ccgacgagat caaggtccgc accccgctga cctgccagac gcgctacggg
     1801 ctgtgcgcca agtgctatgg ccgcgacctc ggccgcggcg gcctggtgaa ctccggcgag
     1861 gcggtcggcg tgatcgccgc ccagtcgatc ggcgagcccg gcacgcagct gacgatgcgt
     1921 accttccaca tcggcggtgc ggcctcgcgt gcggcggtgg cttcgagcgt ggaggccaag
     1981 tccgatggca tcatcggctt caatgccacg atgcgctacg tgaccaacgg caagggcgag
     2041 ctggtggtga tctcgcgctc cggcgagatc atcatctccg accagcatgg ccgcgagcgc
     2101 gagcgccaca aggtgcccta cggcgccacg ctcaacatca aggccgacca gcacatcaag
     2161 gccggcctcg tgctggccaa ctgggacccg ctgacccgac ccatcatcac cgagttcgcg
     2221 ggccgcgcga agttcgagaa cgtggaagag ggcgtgacgg tggccaagca ggtcgacgaa
     2281 gtgaccggcc tgtccaccct agtggtgatc gacccgaagc gccgcggtgc tgccaaggtc
     2341 gtgcgcccgc aggtcaagct gctggatgcg ggcggcaacg aggtcaagat ccccggcacc
     2401 gaccactcgg tgacgatcgg cttccaggtc ggcgcgctga tccaggtgcg cgacggccag
     2461 gacctcgccc ccggcgaagt gctggcgcgt atcccggtcg aaggccagaa g
//
LOCUS       DASUCY010000152         2875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_3498, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000152 DASUCY010000000
VERSION     DASUCY010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3498"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VFV25_02980"
     CDS             <1..273
                     /locus_tag="VFV25_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HEY1392306.1"
                     /translation="QVGIINPSEMSPAAGIEIAIWTAVGGRATVIGPILGALFVNGAK
                     SAFTQLLPELWLYFLGALFIVVTLFLPDGIVGGLRRLFKRAKGADT"
     gene            270..1160
                     /gene="urtD"
                     /locus_tag="VFV25_02985"
     CDS             270..1160
                     /gene="urtD"
                     /locus_tag="VFV25_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HEY1392307.1"
                     /translation="MTPDLMEEGARLLRAHAQRERERQALSGGRDASYGRVVRPDVVD
                     VTHGAILYLDDITVSFDGFRALNDLSLSIDAGELRCIIGPNGAGKTTMMDVITGKTRP
                     DSGSAYFGSTVDLLRLRESEVARAGIGRKFQKPSVFENLCVFENLELALKADRRARAS
                     VLFALSGAQRERIAEVLALIHLKDQARRMAGLLSHGQKQWLEIGMLLMQDPKLLLLDE
                     PVAGMTDEETERTAELFLSLQGEHSLVVVEHDMKFIAALTADDPKKTVTVLNEGSVLA
                     EGPLAQVQANDKVVEVYLGR"
     gene            1258..1950
                     /gene="urtE"
                     /locus_tag="VFV25_02990"
     CDS             1258..1950
                     /gene="urtE"
                     /locus_tag="VFV25_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HEY1392308.1"
                     /translation="MLKVEDLNQYYGGSHIVRNLGFEARVGEVTVVLGRNGVGKTTLL
                     KSLMGLVRIKTGSIKLGGTEMAGLPPYERVRLGVGYVPQGREIFNRLTVAENLSMGLA
                     GQPRGTAVPRELFELFPVLSQMMRRRGGDLSGGQQQQLAIARALAAGPSLLLLDEPTE
                     GIQPSIIKDIGRVIRRLAERKTMAIVLVEQYYDFAAELADQYLVMERGEIVQRGRGID
                     MEADGVRRRISI"
     gene            complement(1955..>2875)
                     /locus_tag="VFV25_02995"
     CDS             complement(1955..>2875)
                     /locus_tag="VFV25_02995"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEY1392309.1"
                     /translation="AGGAAAFPLTARVPPRNPGAEWPGEPGAQVLRTEPGAGEGSLHS
                     PEELKLLVQESREAGILRQAQQEVVARVLDIGDRRIADIMTARPQIEWIDTQDDHANK
                     LRAIRECRHDQLVVARGSLDEPLGIVLKKDLLDQLLDGLALEPMKALREPLIVHEGAS
                     VFGVLEQFKRAPVRMAIVVDEYGNVEGVVTQTDLLEAMAGDLPESEGEEPDIVESEDG
                     SLLIDGLTPAHAAFERLGVREADVQGKVHTIAGFALAQLGHLPRTGETFTFEDWRLEV
                     VDMDGRRIDKLLARREPAPGKVGADRSSGG"
BASE COUNT          473 a          973 c          973 g          456 t
ORIGIN      
        1 caggtgggca tcatcaaccc gagcgagatg tctccggctg cgggcataga gatcgccatc
       61 tggactgccg tgggcggccg cgccacagtg atcggcccca tcctcggcgc cctgttcgtc
      121 aacggcgcaa agagcgcctt cacgcagctg ctgccggagt tgtggctcta cttcctcgga
      181 gccttgttca tcgtcgtgac gctgttcttg cctgacggca tcgttggcgg cctgcgtcgg
      241 ctattcaagc gcgcgaaggg ggctgataca tgacgcccga cttgatggag gaaggtgccc
      301 gcttgctgcg ggcccacgcg cagcgcgagc gggagcggca agcgctctcc ggcggccgcg
      361 atgcgagcta cgggcgcgtg gtgcgacccg atgtggtgga cgtcacccac ggcgccatcc
      421 tctacctcga cgacatcacg gtgagcttcg acggcttccg tgcgctcaac gacctgagcc
      481 tgagcatcga tgccggcgaa ctgcgctgca tcatcggccc caacggcgcc ggcaaaacga
      541 cgatgatgga cgtcatcacc ggcaagacgc ggcccgacag cggcagcgcc tacttcggct
      601 ccaccgtgga cctgctgcgc ctgcgcgaga gcgaggtggc gcgcgccggc atcggccgca
      661 agttccagaa gcccagcgtg ttcgagaacc tgtgcgtgtt cgagaacctc gaactcgcgc
      721 tcaaggccga tcgccgcgcg cgcgcctcgg tgctgttcgc gctgagcggc gcccagcgcg
      781 agcgcatcgc cgaggtgctg gcgctgatcc acctgaagga ccaggcgcgg cgcatggccg
      841 gcctgctctc gcacggtcag aagcagtggc tggagattgg catgctgttg atgcaggacc
      901 ccaagctgct gctgctggac gagccggtgg cgggcatgac cgacgaagag accgagcgca
      961 ccgccgagct cttcctgtcg ctgcagggcg agcattcgct ggtggtggtc gagcacgaca
     1021 tgaagttcat cgccgcgctc acggccgacg atccgaagaa gacggtcacc gtgctgaacg
     1081 agggcagcgt gctggccgaa ggcccgctgg cccaggtgca ggccaacgac aaggtggtcg
     1141 aggtttacct cggcagatga tgacaacccc tcgtccgatg aatacatcgg ccgatccccc
     1201 gaggggatgc gggccggctt ggggcggccc ggcgctcggc ccgcgggaga gcgagacatg
     1261 ctgaaggtcg aagacctcaa ccagtactac ggcggttcgc acatcgtgcg caacctgggc
     1321 ttcgaggcgc gcgtcggcga agtgacggtg gtgctggggc gcaacggcgt gggcaagacg
     1381 acgctgctca agagcctgat ggggctggtg cgcatcaaga ccggcagcat caagctcggc
     1441 ggcaccgaga tggcgggcct gccgccctac gagcgcgtgc gcctgggcgt cggctatgtg
     1501 cctcaggggc gggagatctt caaccgcctc actgtggccg agaacctgag catggggctg
     1561 gccggccagc cgcgcggcac ggccgtgccg cgggagctgt tcgagctgtt cccggtgctc
     1621 agccagatga tgaggcggcg cggcggcgac ctctccggcg gtcagcaaca acagctcgcg
     1681 atcgcccgcg cgctggccgc gggcccgagc ctgctgctgc tggacgaacc caccgaaggc
     1741 atccagccct ccatcatcaa ggacatcggg cgcgtgatcc gccggctggc cgagcgcaag
     1801 accatggcca tcgtgctggt ggagcagtac tacgacttcg cggccgagct ggccgaccag
     1861 tatctcgtga tggagcgcgg cgagatcgtg cagcgcgggc gcggcatcga catggaggcc
     1921 gacggcgtgc gccgacgcat ctcgatctga tgcttcagcc tcctgagctg cgatccgcgc
     1981 cgaccttgcc gggcgccggc tcgcgtcgtg cgagcagctt gtcgatgcgc cggccatcca
     2041 tgtcgacgac ttccagccgc cagtcttcga aggtgaaggt ttctcccgtc ctgggcaggt
     2101 gcccaagctg cgcgagcgca aagccggcga tcgtgtgcac cttgccctgc acatcggctt
     2161 cgcgcacgcc gagccgctcg aacgcagcgt gcgccggcgt aagaccgtcg atcagcagcg
     2221 aaccgtcctc gctctcgacg atgtccggct cctcgccctc ggattccggc aggtcgccgg
     2281 ccatcgcctc gagcagatcc gtctgcgtca cgacgccctc gacgttgccg tactcgtcga
     2341 cgacgatggc catgcgaacc ggagcgcgct tgaactgctc caggacgccg aacaccgatg
     2401 cgccttcatg cacgatcagc ggctcgcgca aggccttcat gggttccagc gccaggccgt
     2461 cgagcagctg gtcaagcaga tccttcttca gcacgatacc aagaggctcg tcgaggctgc
     2521 cgcgcgccac gacgagctga tcgtggcggc actcgcgaat cgcgcgcagt ttgtttgcat
     2581 gatcgtcctg cgtgtcgatc cattcgatct gcgggcgcgc cgtcatgatg tcggcgatgc
     2641 gccggtcgcc gatgtccagt acccgcgcga cgacctcctg ctgcgcctgc ctcaggatgc
     2701 cggcctcccg gctctcctgc accagcagct tcagctcctc cggcgagtgc agcgatccct
     2761 cgccggcgcc gggttcagtc cgcaggacct gagcaccagg ttccccaggc cattcagcac
     2821 caggattgcg gggcggaaca cgagcagtaa gagggaaagc ggccgcacca cccgc
//
LOCUS       DASUCY010000153         2744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1481203, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000153 DASUCY010000000
VERSION     DASUCY010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1481203"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VFV25_03000"
     CDS             <1..397
                     /locus_tag="VFV25_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY1392310.1"
                     /translation="TMKLGYTILYVADVEGSIAFCERAFGLQRGFVHESGAYGELATG
                     ATTLAFAQHETARGNLGHDYVDAAASKQPLGMEIGLVNEDVPAAYQRAVQAGAVALKD
                     PQPKPWGQTIAYVRCPDGLLVELCTPMGD"
     gene            402..1112
                     /locus_tag="VFV25_03005"
     CDS             402..1112
                     /locus_tag="VFV25_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018902999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HEY1392311.1"
                     /translation="MRRADRLFQLVQLVRSRRLSTANWLAERLEVSERTVYRDIADLQ
                     AQGVPIEGEAGVGYRMRAGFDLPPLMFTRDEAQALVAAVRLAQPRLDIALAGQAEAAL
                     GKILAVLPAASRAAAESLAVYAPPAGPDEATRARLQQLRESTEARRKVKLHYLDLRER
                     VSERIVRPLGCFYWGAVWTLAAWCEEREAFRNFRVDRIRQLEVLEERFRDEPGKTLAD
                     LFRQVEAEMDESNRSTDA"
     gene            complement(1146..>2744)
                     /locus_tag="VFV25_03010"
     CDS             complement(1146..>2744)
                     /locus_tag="VFV25_03010"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY1392312.1"
                     /translation="EITARKIRLLEPDHALLGVPGDGGNDVIVAKARWSNDREFRDTL
                     ALRLDTLSGITHPLSAPPDANVTEWMLDEHGEPRAALDTRGAHARLFWRAPGKAGWEL
                     LDDSPTAIGQPRYRPLWNEGGTLYVTAASGKDQTAALYRYETTKRSLDPQALFSTTGF
                     DYDGWHVFDQASGRVVGIRYQSDAAGSYWFDTRLQAAQREIDALLPGTVNRLICLRCT
                     DAARLLISAASDRQPAVYYLFDTKTRKLEAIGSSRPWIDARQMATQDFVRIEARDGLS
                     LPIYVTRPPGKSDAPRPAVVLVHGGPQVRGNVWGWQPITQFLASRGYVVIEPEFRGGN
                     GFGFAHLSAGFRQWGRAMQDDVTDATHWAIRSAGIDPKRICIAGASYGGYAALMGLLR
                     EPDLYRCGFEWVGVTDLELMFSIPYSDLPEDWKQHGLQIMLGDPERDKEAMRAVSPLM
                     QAEHIKQPLLLAYGGRDRRVPLTHGVQFHDAVRRSNRDVEWVVYPEEAHGWGALETNR
                     DFWTRVENFLARNIGAATDTAARR"
BASE COUNT          477 a          927 c          889 g          451 t
ORIGIN      
        1 gacgatgaaa ctcggctaca ccatcctcta cgtcgccgac gtggagggtt ctatcgcctt
       61 ctgcgagcgc gccttcggcc tgcagcgcgg cttcgtgcac gagtccggcg cctatggcga
      121 gctcgccacc ggtgccacca cgctggcctt cgcccagcac gaaacggcgc gtggcaatct
      181 cgggcacgac tacgtggatg cggccgcttc gaaacagccg ctgggcatgg agatcggcct
      241 cgtcaacgag gacgtacccg ccgcctatca gcgtgcggtg caggctggcg cggttgcctt
      301 gaaggatccg cagcccaagc cctggggcca gaccatcgcc tatgtgcgct gccccgacgg
      361 gctgctggtc gagctgtgta cgcccatggg ggattgaacg cgtgcgccgt gccgaccgcc
      421 tgtttcagct cgtgcaactg gtgcgcagcc ggcgactgtc gaccgcgaat tggctcgccg
      481 agcggctgga ggtttccgag cgcaccgttt accgcgacat cgccgacttg caggcccagg
      541 gcgtaccgat cgaaggcgaa gccggcgtgg gctaccgcat gcgcgccggt ttcgacctgc
      601 cgccgctgat gttcacgcgc gacgaagccc aagccctggt ggccgccgtg cgcctggccc
      661 agccgcgcct ggacatcgcg ctggctggcc aggccgaggc cgcgctgggc aagatcctgg
      721 ccgtgctgcc ggccgcctct cgcgccgccg ccgagagcct tgccgtctat gcgccgccgg
      781 ccgggcctga cgaggccacg cgcgcacgcc tgcagcaact gcgcgagtcc accgaggcaa
      841 ggcgcaaagt caagctgcac tacctcgacc tcagggagcg cgtcagcgaa cgcatcgtgc
      901 ggccgctggg ctgcttctac tggggcgccg tctggacctt ggccgcgtgg tgcgaggagc
      961 gcgaggcgtt tcgcaatttc cgcgtcgacc gcatccggca gctcgaagtg cttgaggagc
     1021 gcttccgcga cgaacccggc aagaccctgg ccgacctgtt ccgccaggtc gaagccgaga
     1081 tggacgagag caatcgcagc acggacgcgt gagtgcagga gcgggtgccc gccggcctca
     1141 ggtcgtcaac gtcgcgccgc cgtatccgtc gcagcgccga tgttgcgtgc aagaaaattt
     1201 tcaacccgcg tccagaaatc gcgattcgtc tccagagcgc cccagccatg agcttcctcg
     1261 ggatagacga cccattcgac atcgcggttg ctacgcctca cggcatcgtg gaattgcacg
     1321 ccatgagtca gcggaacgcg tctgtctcgg ccgccatagg cgagcagcag cggctgcttg
     1381 atatgctcgg cctgcattag cggcgacacc gcccgcatcg cctccttgtc ccgctcggga
     1441 tcgcccagca tgatctgcag accgtgctgc ttccagtcct cgggcaggtc ggagtaaggg
     1501 atcgagaaca tgagttccag atccgtcacg cccacccact cgaagccgca gcgatacaaa
     1561 tcaggctcgc gcagcaaccc catcaacgcc gcgtagccgc cgtagctggc cccagcgatg
     1621 caaatgcgct tggggtcgat gccggcactg cgtatcgccc aatgcgtggc atcggtcaca
     1681 tcgtcttgca tcgcccgccc ccattggcgg aagccggcag acaggtgggc aaagccgaag
     1741 ccgttgccgc cgcgaaactc aggctcgatg acgacatagc cgcgcgaagc gagaaactga
     1801 gtgatcggtt gccaacccca gacattgccg cgaacctgcg gaccgccatg caccagcacc
     1861 actgcgggtc gcggagcatc gctcttgccg ggcggacgcg tgacatagat cggcaagctc
     1921 aggccatcgc gcgcctcgat gcgcacgaaa tcctgcgtag ccatctgtcg cgcatcgatc
     1981 caaggcctgg acgaaccgat cgcctccagc ttgcgggtct tggtgtcgaa caggtagtag
     2041 accgccggct gacgatccga tgctgcgctg atcagcaggc gcgcggcgtc ggtgcagcgc
     2101 aagcagatga gacgattgac ggtgccaggc aacagagcat cgatctcgcg ctgggccgct
     2161 tgcagccgag tatcgaacca gtagctgcca gcggcatcgg attgatagcg gatgcctact
     2221 acgcgcccgc tggcctggtc gaagacgtgc cagccgtcat agtcgaaccc cgtggtcgaa
     2281 aacaaggcct gagggtcgag actgcgcttg gtcgtctcgt agcggtacag ggcagcggtc
     2341 tggtccttgc cgctggctgc ggtcacgtag agcgtgccac cttcgttcca cagcggcctg
     2401 tagcgaggct gaccgatggc ggtcggagag tcatccagca actcccagcc ggctttgcca
     2461 ggagcgcgcc agaagaggcg tgcgtgcgcg ccgcgggtat ccagcgctgc gcgcggctcg
     2521 ccgtgctcgt cgagcatcca ttcggtgacg tttgcatccg gcggcgcgct caggggatgc
     2581 gtgatgccac tgagggtatc gaggcgcagc gccagcgtgt cgcggaactc acgatcgttg
     2641 ctccagcgag ccttggccac gatcacatcg ttgccgccat caccaggcac tcccaacaaa
     2701 gcatgatcag gttccagcag cctgatcttg cgcgccgtga tctc
//
LOCUS       DASUCY010000154         2528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1208477, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000154 DASUCY010000000
VERSION     DASUCY010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1208477"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..1690)
                     /gene="murJ"
                     /locus_tag="VFV25_03015"
     CDS             complement(122..1690)
                     /gene="murJ"
                     /locus_tag="VFV25_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEY1392313.1"
                     /translation="MNLLRAASVVSLLTLASRITGLVRELLMASVFGASAMTDAFNVA
                     FRIPNLFRRVLGEGAFSQAFVPVLAATRSEGGEAGAKQLIDHVGTLLSWVLLLFCLAG
                     MLAAPAMVWGMASGLEKTPRGYEAAVFMTRWMFPYIGFMSLVALAGGILNTWRRFAVP
                     AASPVLLNVALILSITLGAPWFARHGIEPIYAQAAGVMVGGVLQLGLQIPALAKLGLL
                     PRLGASLAALRAAWADPTTRKIARLMLPALLGVSVAQLSLLINTQIASHLASRSITWI
                     TYADRLMEFPTALLGVALGVVLMPQLASARAEHDDARYSALLDWGLRLVVLLSVPCAV
                     ALVLFSTPLVATLYHHGEFSELDVQRVSAALMNYGFGLLGIVAIKVLAPGYYARHDTR
                     TPMLIGVSVLVFTQLLNIVFVPLLQHAALTLTIAIGAMVNALWLLLGLMRRGSYKPEP
                     GWAVFALRVALASAAMGAGLSYAAHELDWVGLGAHQWLRTGWLALVLGGAAVLYFGVL
                     LMSGLKLRSFIKRA"
     gene            1858..2154
                     /gene="rpsT"
                     /locus_tag="VFV25_03020"
     CDS             1858..2154
                     /gene="rpsT"
                     /locus_tag="VFV25_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEY1392314.1"
                     /translation="MATASKAKKKTVRLASGRKRARQDVKLNAANTSLRSKFRTAIKG
                     VLKAVTAGDKTKATETFKAAQSVIDSIADKGLFHKNKAARYKSRLSAKVKALAA"
     gene            complement(2223..>2528)
                     /locus_tag="VFV25_03025"
     CDS             complement(2223..>2528)
                     /locus_tag="VFV25_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3579 domain-containing protein"
                     /protein_id="HEY1392315.1"
                     /translation="REVFIQGLTLDGKTFRPSDWAERLAGAMSCFRPGGVAQGRAAHI
                     GYSPYCVPTVIGGVKCVIVNEALRDVEPMAWDFVMNFARDNRLQVAEACLLPDSPKT"
BASE COUNT          490 a          872 c          844 g          322 t
ORIGIN      
        1 gccaggctcg cgtcatcggc gaccagctgg gagaagtact ccagcggcga cacgcgggct
       61 agtatcggca gagccatgga tgcagtgtag gactgccgca gcgccgcagc gacccccgga
      121 atcaggcgcg cttgatgaag ctgcgcagct tgaggccgct catcagcagc acgccgaaat
      181 aaagcacggc cgccccgccc agcaccagcg ccagccagcc ggtgcgcagc cactggtgcg
      241 cgcccaggcc gacccagtcc aattcatggg ccgcatagga gaggcccgct cccatcgcgg
      301 ccgaagccag cgccacgcgc agcgcgaaga cggcccagcc tggctcgggc ttgtagctgc
      361 cgcgccgcat caggcccagc agcagccaca gcgcgttgac catggcgccg atcgcgatgg
      421 tcagcgtcag cgcggcatgc tgcagcagcg gcacgaagac gatgttgagc agctgcgtga
      481 acaccagcac gctgacgccg atcagcatcg gcgtgcgcgt gtcgtgccgc gcgtagtagc
      541 cgggcgccag caccttgatc gcaacgatgc cgagcaggcc gaagccgtag ttcatcagcg
      601 ctgccgagac gcgctgcacg tcgagctcgc tgaactcgcc gtggtggtag agcgtcgcca
      661 ccagcggcgt cgagaacagc accagcgcca ccgcgcaggg caccgagagc agcaccacca
      721 ggcgcaggcc ccagtcgagc agggccgaat agcgcgcgtc gtcgtgctcg gcgcgcgcgg
      781 aggcgagctg gggcatcagc accacgccta aagcgacgcc gagcaaggcg gtggggaatt
      841 ccatcagccg gtcggcatag gtgatccagg tgatgctgcg cgacgcgagg tgggaggcga
      901 tctgcgtgtt gatcagcagc gagagctggg ccacgctgac gcccagcagg gccggcagca
      961 tcagccgggc gatcttgcgc gtcgtcggat cggcccaggc cgcgcgcaag gccgccagac
     1021 tcgcacccaa gcgcggcagc aggccaagct tcgccagggc cgggatctgc aaccccagct
     1081 gcagcacgcc gcccaccatc acgccggccg cctgtgcata gatgggctcg atgccgtgcc
     1141 gcgcgaacca gggcgcgccc agcgtgatcg acaggatcag cgccacgttg agcagcactg
     1201 gcgaggcggc cggcaccgcg aagcgccgcc aggtgttgag gatgccgccg gccagcgcca
     1261 ccagcgacat gaagccgatg taggggaaca tccagcgcgt catgaagacc gccgcctcgt
     1321 agccgcgcgg cgtcttctcc aggccgctgg ccatgcccca gaccatggcc ggcgccgcca
     1381 gcatgccggc caggcagaac agcagcagca cccagctcag cagcgtgccc acatggtcaa
     1441 tcaactgctt ggcgcctgcc tcgccccctt cgctgcgggt cgccgccagc accggcacga
     1501 aggcctggct gaaggcgccc tcgcccagca cgcggcgaaa caggttgggg atgcggaagg
     1561 cgacgttgaa agcgtcggtc atcgcgctgg ccccgaagac cgaggccatc agcagctcgc
     1621 gcacgaggcc tgtgatgcgc gacgccaggg tgaggaggga gacgacggat gcggcgcgca
     1681 gcaggttcat gcgaggaagg aattaggccc ccagggccgg gctacgccgg gcatttcctt
     1741 gagaggcggc ggcggattat aggaagtgac tactaacgcg cctgggttgc ctagcacttg
     1801 acaagcctgc cgtgctatac tttcaggctt tccacccacc tggacacccg tagacacatg
     1861 gccaccgcct ccaaagccaa gaaaaagacg gttcgactcg cgtcgggccg caagcgcgcg
     1921 cgtcaggacg tcaagctgaa cgccgccaac acctcgctgc gctccaagtt ccgcaccgcc
     1981 atcaagggcg tgctcaaggc cgttacggcc ggcgacaaga ccaaggccac cgagaccttc
     2041 aaggccgccc agagcgtcat cgactccatc gccgacaagg gcctcttcca caagaacaag
     2101 gccgctcgct acaagagccg cctctcggcc aaggtaaagg cgctggctgc ctgaagccag
     2161 atcgtccagg tggaaacgac aaggcccgca actgcgggcc ttgtgctttt tggcctcccc
     2221 gttcaggtct tgggggagtc aggaagcaga caggcctcgg caacctgtag ccggttgtcg
     2281 cgcgcgaagt tcatcacgaa gtcccaggcc atcggctcga cgtcgcgcag cgcctcgttg
     2341 acgatcacgc atttcacgcc gccgatcacg gtgggcacgc agtagggcga gtagccgata
     2401 tgggccgccc ggccctgggc tacgccgccc gggcggaaac aggacatggc gcccgccagg
     2461 cgctcggccc agtcgctggg gcgaaaggtc ttgccatcca gggtcaggcc ctggatgaag
     2521 acctcgcg
//
LOCUS       DASUCY010000155         2239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_822736, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000155 DASUCY010000000
VERSION     DASUCY010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822736"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(230..589)
                     /locus_tag="VFV25_03030"
     CDS             complement(230..589)
                     /locus_tag="VFV25_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEY1392316.1"
                     /translation="MNNDITLSSQGLNKVVKGRQAADCTSLEQLPNIGPSLAADLRLI
                     GVESPRDLIGKDAFVLYQKLCAITGKRHDPCVLDTFMAVTDFMRGAAPAPWWQYTAQR
                     KALYGQIPEQTETSSCR"
     gene            complement(601..1290)
                     /gene="lexA"
                     /locus_tag="VFV25_03035"
     CDS             complement(601..1290)
                     /gene="lexA"
                     /locus_tag="VFV25_03035"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEY1392317.1"
                     /translation="MDEAPKLTARQQQILELIQSSIERSGAPPTRAEIAAELGFRSAN
                     AAEEHLQALARKGVIELVGGTSRGIRLKSDTLRSIHASRLSGIGLQFSLPLPSLAQLS
                     LPLVGRVAAGQPILAQEHIDQTYVVEASMFPRKPDYLLKVRGMSMRDAGILDGDLLAV
                     QKAKDAKNGQIVVARLNDEVTVKRFRRTKAGIELIPENPEFQTLVVTPESGEFELEGL
                     AVGLIRNTMLM"
     gene            1387..>2239
                     /locus_tag="VFV25_03040"
     CDS             1387..>2239
                     /locus_tag="VFV25_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEY1392318.1"
                     /translation="MAVGKASITVILGTGGTIAGKAADAGDNIGYTAAQIGVAQLLAA
                     VPALSGLPLEAEQVAQLDSKDMSFEVWRRLAERVSHHCARPEVSGVVVTHGTDTLEET
                     AYLLQRVLSPSKPVVMAAAMRPATAMQADGPQNLLDAVSVARHAGVHGVVAVLAGAVW
                     SGGELRKSHTYRLDAFNAGDAGPLAWVEEGALRRLRPWPEGRPLGLDLLAREPWPRVE
                     IVWSHAGADGRLVDALVADGVNGIVVAATGNGTLHHALEEALLRAQQRGVKVLRSTRV
                     AAGAVLAK"
BASE COUNT          352 a          671 c          819 g          397 t
ORIGIN      
        1 ttgtgcttgg cgacttgcgc gcgaggatga ggtaagaccg gcgagccggg cggccatgcg
       61 aacgaggcga atcatgatgg gcatctccga agttcaagaa aaagctatga agttcggcag
      121 gattggcatc gtctcagacc atctgcggga tcgcatcgca gtgccggctt ggcgccacga
      181 tgtatgcccg ccacccgcag aggccgcaag ctgttgaaga gaagccgact cagcggcagc
      241 tgctggtctc cgtttgttcg gggatctggc cgtacagcgc cttgcgctgc gctgtgtatt
      301 gccaccaggg ggccggggcc gcgccgcgca tgaaatcggt cacggccatg aaggtatcga
      361 gcacgcaggg gtcgtgccgc ttgccggtga tggcgcacag cttctggtag agcacgaagg
      421 cgtccttgcc gatcaggtcc cgagggctct cgacgccgat aaggcgcagg tctgcggcca
      481 gggacgggcc gatgttgggc aactgctcga gcgacgtgca atccgccgcc tgcctgccct
      541 tgacgacctt gttgaggcct tgactggaaa gggtgatgtc gttgttcatg cgcagctcgc
      601 tcacatcagc atggtgttgc ggatgagtcc cacggccagg ccttcgagct cgaattcgcc
      661 gctctcgggt gtcacgacca gggtctggaa ctcggggttc tcgggaatca gctcgatgcc
      721 ggccttggtg cggcggaagc gcttgaccgt gacttcatcg ttcaggcggg ccacgacgat
      781 ctggccgttc ttggcgtcct tggccttctg cacggccagc aggtcgccat cgaggatgcc
      841 ggcgtcgcgc atgctcatgc cgcgcacctt gagcagatag tcgggcttgc gcgggaacat
      901 gctggcctcg actacatagg tctggtcgat atgttcctgt gcgaggatgg gctggccggc
      961 ggcaacgcgc ccgaccagcg gcagcgagag ttgggccagg ctgggcagag gcagcgagaa
     1021 ctgcaggccg atgccgctca ggcgcgaggc atggatggag cgcaatgtgt cagacttcag
     1081 ccgtatgccg cgcgaggtgc cgccgacgag ttcgatgaca cccttgcgtg ccagggcctg
     1141 caggtgctcc tcggcggcat tggccgagcg aaagccgagc tcggcggcga tctcggcccg
     1201 ggtgggcggc gcgccgctac gctcgatgct gctttggatc agctcgagga tctgctgctg
     1261 gcgcgcggtg agtttgggcg cttcgtccat ggcggggagt ccggttcggc ctgaggtcaa
     1321 gtttctgctg gctggctatc catccagtat ctgtattttt atccagtcct gagaaaaatg
     1381 caaagcatgg ctgtaggcaa ggcttcgatc accgtcatcc tgggcaccgg cggcaccatc
     1441 gccggcaagg cggcggacgc cggcgacaac atcggctaca cggccgcgca gatcggcgtg
     1501 gctcagctgc ttgcagcggt gccggccctg agcgggctgc cgctggaagc cgagcaggtg
     1561 gcccagctcg acagcaagga catgagcttc gaggtctggc gccggcttgc cgagcgtgtg
     1621 agccaccatt gcgcgaggcc tgaggtgtcc ggcgtggtcg tgacgcacgg caccgacacg
     1681 ctggaggaaa cggcctatct gctgcagcgc gtgctgagcc cgtccaagcc ggtcgtgatg
     1741 gccgctgcaa tgcgcccggc cacggcgatg caggccgatg gcccgcagaa tctgctggac
     1801 gccgtcagcg tggcccgcca tgccggcgtg catggcgtcg tggccgtgct ggccggcgcg
     1861 gtctggtcag gcggcgagct gcgcaagagc catacctaca ggctggatgc cttcaatgcg
     1921 ggcgatgcgg ggccgctggc ctgggtcgag gagggcgcgc tgcgcaggct caggccctgg
     1981 cccgagggcc ggccgctggg cctggatctg ctggcgcgcg agccttggcc gcgtgtcgag
     2041 atcgtgtgga gtcatgccgg cgccgatggc aggctggtcg atgcgctggt ggccgatggg
     2101 gtgaacggca tcgtcgtggc cgccaccggc aacgggacct tgcatcacgc gctggaagag
     2161 gcgctgttgc gggcgcagca gcgcggagtg aaagtgctgc gcagcactcg tgtggcagcc
     2221 ggcgccgtgc tggccaagc
//
LOCUS       DASUCY010000156         5377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1368981, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000156 DASUCY010000000
VERSION     DASUCY010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 5377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5377
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1368981"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..1096
                     /locus_tag="VFV25_03045"
     CDS             95..1096
                     /locus_tag="VFV25_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HEY1392319.1"
                     /translation="MLVTLLLFALSAGAIYFACEFFVNGVEWLGHRLNLSATATGTVL
                     AAFGTALPESAVTFVAVAMGTTPAQKDIGVGAALGGPLVLATIAYAVVGFALLANRSR
                     RQEQAGRHEVAVDQRRLSRDQAWFMAIFTVKTGLGLVVFTFKPWLGVLFLAAYAIYVW
                     RELRDEGAADHEDFEPLKIRPRDTSPSLFWVSLQTVAALGVVAAASHIFVIQLESLGI
                     AFGMSPHLVALLLSPVATELPETMNALIWVRQGKERLALANISGAMMIQATIPSALGL
                     FFTPWLFDAPLMAAAVVTMVAVVFLWSLFRQGVMDSRMLACAGTLYPVFAGYVFFFS"
     gene            complement(1140..2324)
                     /locus_tag="VFV25_03050"
     CDS             complement(1140..2324)
                     /locus_tag="VFV25_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEY1392320.1"
                     /translation="MSDTAEAPQAPATGYTLWQLVRYMLGLGTWGFGGPVALLGYMYR
                     DLVEKRGWISESDYKEGLTLAQLMPGPMAAQLAIYLGFVHFRVLGATLVGLAFVLPSF
                     LMVVALGAVYVTYGGIAWMQAVFYGVGAAVIGIIAVGAYKLTTKNIGKDKLLWVIFIG
                     NAVLTAVTQTEYVWTVLGAGLLVWLLRAAPRAWSGGRLNGVAAPILGVFAFASADPHR
                     LAQIGAYFAYAGSFVFGSGLAIVPFLYGGVVKEYGWLTDKQFVDAVAVAMITPGPVVI
                     TTGFIGFLVSGFWGAVVAAAGTFLPCYLLTIVPAPYFKKHGKRPGLLAFINGATAAAI
                     GAIAGSVIVIGQRSITDWLTALLAVATAGVLWRFKRVTEPVIVVVAALVGLVAYPLLS
                     HG"
     gene            complement(2339..3301)
                     /locus_tag="VFV25_03055"
     CDS             complement(2339..3301)
                     /locus_tag="VFV25_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HEY1392321.1"
                     /translation="MDTQPHWLLLIVSLPTPSATARMRVWRGLKGLGCMALRDGAYLL
                     PAGEEREPALRELGDECTREGGIAWLMAVLPRSADEELAYRQLFDRSEDYADLRKSWK
                     EANRGLAGLSPTELARLQRKLQREYEAVRAIDFFRGDASADAESAWTDLNRRVEQLLS
                     PDEPHETKGGIPRLDAQQYRGRTWATRRRLWVDRVASAWLIRRFIDPEARFKWLARPS
                     DCPKSALGFDFDGATFTHIGDRVSFETLLASFGLEDDPALLRLGAMVHQLDVGGDPVP
                     EAIGFEAVMAGARERLDDDDALLAEMTMVLDSLYAHFGRQADKA"
     gene            3380..3673
                     /locus_tag="VFV25_03060"
     CDS             3380..3673
                     /locus_tag="VFV25_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392322.1"
                     /translation="MQVFDTVSGARVAELPLCGDADDLFLDAERRRLYAVCGDGRVAI
                     IGQRDGDRYDTVQNIATSPGARTGLWVQATKTLFVAAPASQGSPAAVLVYRLE"
     gene            3836..5377
                     /locus_tag="VFV25_03065"
     CDS             3836..5377
                     /locus_tag="VFV25_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008250973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 domain-containing protein"
                     /protein_id="HEY1392323.1"
                     /translation="MKKAPLSTLAAACGAVALLAVATPACASSHREAPFITTAPKVDA
                     TDFYMFNSYEAGRAGYVTLIANYQPFQEPFGGPNYYMMDPNALYEIHIDNDGNAREDL
                     SFQFRFKNTLANGGQGVALPIGGAQVAIPLIQAGSVSSLMDPHLNVAETYTLTLMRGD
                     RRDGQAAAVTNADTGSATFEKPVDNIGVKTIPDYAAYAAKHVYNINIPGCGIPGKVFV
                     GQREDPFWVNLGPIFDLVNLPVAVLTDPSKINAFPNSLAGKNVTTLALEVHKSCLTKN
                     DDVIGAWTTASLRQARLLDGRPPSGHHTSEKPGGAWVQVSRLGMPLVNEVVIGLKDKD
                     KFNASKPRDDAQFATYVTNPTLPALLEIALGLPGISPTNIPRNDLVTTFLTGIAGLNQ
                     PRNVVASEMLRLNTAVPAVSFAQQNRLGIVGNILAGGNDNAGFPNGRRPKDDVVDISL
                     VAVMGGLCVANGNNNALGFGVACNPSAVPLGKTSFNLNDGVDQAQLTLLPGFPYLNTP
                     FGGTK"
BASE COUNT          941 a         1843 c         1708 g          885 t
ORIGIN      
        1 gatcggcgag acctttggcc gcagcgtgaa ccgtcagccg gacgagccac gttgccgtgc
       61 catcggttta ttgcttgtcc ggcgcccacg ttccatgctc gtcacactgc tcttgttcgc
      121 cttgtccgcg ggcgcgatct atttcgcctg cgaattcttc gtcaatggcg tcgaatggct
      181 cggccaccgc ctgaacctca gcgctaccgc cactggcacc gtgctggcag cttttggtac
      241 ggcgctgccg gaaagcgccg tcaccttcgt ggccgtggcg atgggcacga cgccagcgca
      301 aaaggacatc ggtgtcggcg cggctctcgg cgggccgctg gtcctcgcca ccatcgccta
      361 tgcggtcgtc ggcttcgcgc tactcgccaa tcgcagcaga cggcaggagc aggcgggtcg
      421 gcacgaagtg gctgttgacc agcgcagact gagtcgggat caagcctggt tcatggcgat
      481 cttcacggtg aagacgggcc tgggcctggt ggtcttcacc ttcaagccct ggctcggtgt
      541 gctgttcctg gcagcctacg cgatctatgt ctggcgggaa ctccgcgacg aaggcgccgc
      601 cgaccacgaa gacttcgagc cgctcaagat ccggccccgg gacaccagcc cgagcttgtt
      661 ctgggtgtcg ttgcaaacgg tcgcagcctt aggcgtggtc gcggcggcgt cccacatctt
      721 cgtcatccaa ctcgaatcgc tcgggatcgc ctttggcatg tcgccccact tggtggcgct
      781 gctcttgagc ccggtggcca ctgagctccc ggaaacgatg aatgcactga tctgggtccg
      841 ccagggcaag gagcggctgg cgctggccaa catctccggc gccatgatga tccaggcgac
      901 gatcccgagc gcgctgggac tgttcttcac gccgtggctg ttcgatgcgc cgctgatggc
      961 cgccgcggtg gtcacgatgg tggccgttgt ttttctgtgg tcactgtttc ggcagggcgt
     1021 gatggacagc cggatgctgg cctgcgcggg cacgctgtat ccggtattcg ctggttacgt
     1081 cttcttcttc agctgacgcg cacggtcatg cccgaaggcc gcgtgcgacc ctgcgtcttt
     1141 cagccatgcg aaagcaaggg ataggcgacc agaccgacca gcgccgcaac gacgacgatt
     1201 accggctcgg tcaccctctt gaagcgccaa agtacgcccg cggtggccac cgccagcagc
     1261 gcggtgagcc agtcggtgat cgagcgctgg ccgatcacga tgaccgagcc ggcgatcgcg
     1321 ccgatggccg cagcggtcgc gccgttgatg aaggccagca gccccgggcg cttgccatgc
     1381 ttcttgaaat agggtgccgg cacgatggtc agcaggtagc acggcaggaa ggtgccggcg
     1441 gcggcgacca ccgcgcccca gaagccggag accagaaagc cgatgaagcc ggtcgtgatg
     1501 acgaccggcc ccggcgtgat catcgccacc gccaccgcgt cgacgaactg cttgtcggtc
     1561 agccagccgt attccttgac gaccccgccg tagaggaagg gcacgatcgc caggccgctg
     1621 ccaaagacga agctgcccgc ataggcgaag taggcgccga tctgcgcgag cctgtgcgga
     1681 tcggcactgg cgaatgcgaa aacacccagg atcggggccg cgacgccatt gaggcggccg
     1741 ccggaccagg ctctgggcgc tgcgcgcagc agccagacga gcaggcccgc gccgagtacg
     1801 gtccagacgt actcggtctg cgtcacggcc gtcaacacgg cgttgccgat gaagatgacc
     1861 cacagcaact tgtccttgcc gatgttctta gttgtgagct tgtaggcgcc cactgcgatg
     1921 atgccgatca ccgcggcgcc cacgccatag aacaccgcct gcatccaggc tatgccgccg
     1981 taggtcacat agaccgcgcc gagcgccacc accatcagga acgaaggcag tacgaaggcc
     2041 aggccgacca aggtggcgcc gaggacacgg aagtggacaa agccgaggta gatggccaac
     2101 tgcgcggcca tggggccggg catcagctgc gccagcgtca agccttcctt gtagtcgctt
     2161 tcggagatcc agccgcgctt ctcgaccagg tcgcggtaca tgtagcccag cagcgccacc
     2221 ggcccgccaa agccccaggt gcccaggccc agcatgtagc gcacgagctg ccacagcgtg
     2281 tagccggtag cgggagcctg gggcgcttcg gctgtatcgc tcattgctcg ctttctgact
     2341 aagctttgtc ggcctggcgc ccgaaatgcg cgtacagcga gtcgagcacc atggtcatct
     2401 ccgcgagcag cgcgtcgtcg tcgtcgagcc gctcgcgggc gccggccatt acggcttcga
     2461 agccgatcgc ctccggtacc ggatcgccgc ccacatcgag ctgatggacc atggcgccca
     2521 gtcgtagcaa cgccgggtcg tcttccagtc cgaagctcgc cagcagggtt tcgaaactga
     2581 cccggtcgcc gatatgcgtg aacgtggcgc catcgaaatc gaagccgagt gcactcttgg
     2641 ggcagtccga gggcctcgcc aaccacttga agcgagcttc gggatcgatg aagcgtcgga
     2701 tcagccaggc gctggccacc cgatcgaccc atagccgccg gcgcgtggcc caggtgcggc
     2761 cgcggtactg ctgtgcatcg aggcgaggga tacccccctt cgtctcatgg ggctcgtccg
     2821 gcgacagcag ctgctcgacg cgacggttga ggtccgtcca ggcggactcc gcgtccgcgc
     2881 tggcgtcgcc gcgaaagaag tcgatcgcgc gcacggcctc gtactcgcgc tgcagcttgc
     2941 gctgcaggcg cgccagctcg gtgggcgaca agccggcgag gcctcggttg gcctccttcc
     3001 agctcttgcg caggtcggcg tagtcctcgc tgcggtcgaa caactggcgg taggccagct
     3061 cctcgtccgc cgagcgcggc agcaccgcca tcagccaggc gatgccgcct tcgcgcgtgc
     3121 actcgtcgcc aagctcgcga agcgccggtt cacgctcctc gccggccggc agaaggtagg
     3181 cgccatcgcg cagtgccatg cagcccaggc ccttgagacc gcgccaaacg cgcatgcgcg
     3241 ccgtcgcgct gggcgtcggc agcgacacga tgagcagcag ccaatgcggc tgcgtatcca
     3301 tagccatgtc gcgaatataa ctcgaatgtc gcatatgcta cataacatga ccctgcgagc
     3361 cacacgcgct ccagcgagct tgcaggtctt cgataccgta tcgggtgcgc gggtcgccga
     3421 gttgccgttg tgcggcgacg ccgacgatct gttcctcgat gccgagcggc ggcgccttta
     3481 cgcggtgtgc ggggatgggc gagtcgccat catcggtcag cgcgacggcg accggtacga
     3541 cacggtccag aacatcgcca catcgccggg ggcacgcact ggcttgtggg tgcaggcgac
     3601 gaagacgctg ttcgtggccg cgccggcttc gcagggaagc ccggcggctg tcctcgtcta
     3661 ccgcctcgag tgaggcgcat tgcattgggc tttcgcgatg cgcggcgagc ctccaaaggc
     3721 cctgcagcca acgcagggat tgccaggctg catccgctac tcccgccgca gcgtaaagcg
     3781 cagtgcacgg ccgtaggccg cggcacgcga atgctgcaac tcccaggaga cccgcatgaa
     3841 aaaagctcct ctctccaccc tcgccgcagc ttgcggcgcc gtcgccctgc tggcagtcgc
     3901 caccccggcg tgcgcctcca gccaccgcga ggcgcccttc atcaccactg cgcccaaggt
     3961 cgacgccacc gatttctaca tgttcaacag ctacgaggct ggccgcgccg gctatgtgac
     4021 gctgatcgcc aactatcagc ctttccagga acccttcggc gggcccaact actacatgat
     4081 ggaccccaac gcgctgtacg aaatccacat cgacaacgac ggcaacgcgc gcgaggacct
     4141 cagcttccag ttccggttca agaacacgct cgccaacggc ggccagggcg tggcgctgcc
     4201 tattggcggc gcccaggtgg cgatcccgct gatccaggcg ggcagcgtgt ccagcctgat
     4261 ggatccgcat ctgaacgtgg ccgagaccta taccctgacg ctgatgcgcg gcgaccggcg
     4321 cgacggtcag gctgcggcgg tgaccaatgc cgacaccggc agcgccacct tcgagaaacc
     4381 ggtggacaac atcggcgtca agaccatccc cgactatgcc gcctacgccg ccaaacacgt
     4441 ctacaacatc aacatccccg gctgcgggat accgggtaag gtcttcgtcg gtcagcgcga
     4501 ggacccgttc tgggtcaatc tgggcccgat cttcgatctg gtcaatctgc cggtcgccgt
     4561 gctcaccgac ccgagcaaga tcaacgcctt ccccaacagc ctggccggca agaacgtcac
     4621 gacgctggcg ctggaggtgc acaagagctg cttgaccaag aacgacgacg tgatcggcgc
     4681 ctggaccacg gccagcctgc gccaggccag actgctcgac ggccgtccgc cctcgggcca
     4741 ccacaccagc gagaagcccg gcggtgcctg ggtgcaggtt tcgcgcttgg gcatgccgct
     4801 ggtcaacgaa gtggtgatcg gcctgaagga caaggacaaa ttcaacgcct ccaagccccg
     4861 cgacgacgct cagttcgcca cctacgtgac caaccccacg ctgcccgcgc tgctggagat
     4921 cgcactcggc ctgcccggca tctcgccgac caacatcccg cgcaatgacc tagtcacgac
     4981 cttcctcaca ggcattgctg gcctcaacca gcccaggaac gttgttgcct ccgagatgct
     5041 gaggctcaac accgcggtcc ccgccgtgtc cttcgcgcag cagaaccggc tgggtatcgt
     5101 tggcaacatc ctcgcgggtg gcaacgacaa cgcgggcttc cccaacggcc gccgccccaa
     5161 ggacgatgtg gtcgacatct cgctggtcgc ggtcatgggc gggctgtgcg tggccaacgg
     5221 taacaacaac gcgctgggct tcggcgtggc ctgcaacccc tcggccgtgc cgctgggcaa
     5281 gacgtcgttc aacctgaacg acggcgtgga ccaggctcag ctgacgctgc tgccgggctt
     5341 cccttatctc aacacgccct tcggcggcac gaagtga
//
LOCUS       DASUCY010000157         4636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1593687, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000157 DASUCY010000000
VERSION     DASUCY010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1593687"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            315..1499
                     /locus_tag="VFV25_03070"
     CDS             315..1499
                     /locus_tag="VFV25_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEY1392324.1"
                     /translation="MTLRARPAFSSATRPIARGLAVAAALGLLGAGAAWGQKGPNYPI
                     TPAQRSTAQQVAEAGVPLSELAANAPDSYTVKRGDTLWGISSIYLKRPWRWPELWGMN
                     MDQIRNPHLIYPGQVLYLEKTGDRARLRAGAAGGGTVKLSPRVRSSDAYTAIASVPLH
                     LIEPFLNEAVIFETNELERAPRIVASQEGRVFLGRGDLAYVRGDLNDERSWRLFRNPK
                     PLTDPSTGEVLGYEAAYVGTAQYLRPGDTRTIDGKPEIIPATFVIGDIKQEAGVGDRL
                     APVPARDFTNYVPHAPSSPIVGQIASLYGEALSAGQNQIVTLNKGASDGLERGHVLAL
                     WRDGSRAYDPTQGGDRGEPIKLPDERHGLLYVFRVFNRMSYALILSVKDPVRVGDKFT
                     QP"
     gene            1540..2640
                     /gene="dprA"
                     /locus_tag="VFV25_03075"
     CDS             1540..2640
                     /gene="dprA"
                     /locus_tag="VFV25_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEY1392325.1"
                     /translation="MDRDELAAWLRLLETPQIGPVAARKLLAALGSPQAIFEASAGAL
                     RGLLGEKEAQALQTAPESLPALLDATWTWLQTEPGRHLLTLGDADYPAALLECADPPL
                     LLYAIGRLETLRRPALAVVGSRNPTPQGLQNATAFAESLSRAGLAIVSGLALGIDGAA
                     HEGALRAAGSTIAVVGTGLDRVYPKQHRELAHRIGERGLLLSEYPLGMPPLAPNFPKR
                     NRIISGLARGTLVVEAALQSGSLITARLAAEMGREVFAIPGSIHAPQSRGCHALIEQG
                     AKLVDSAQDVLEELRLELAAVPIAPSVPAAAGAAKDAVLDALGYDPVNLDALSARTGM
                     DPAALSARLLELELEGQVARLPGQLFQRLARG"
     gene            2721..3173
                     /locus_tag="VFV25_03080"
     CDS             2721..3173
                     /locus_tag="VFV25_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HEY1392326.1"
                     /translation="MFDVLVYLYENYWRPDACPDHAQLTRKLSAVGFESEEIQEALTW
                     LDGLASAAESHHGDQGELSLRHYTAAEHDHMGEESIGFISFLESAGVLRPPMREIIVD
                     RAMAISGGPVALEDLKIIVLMVFWSLGEEPDALILDELFVDVEDRLIH"
     gene            complement(3267..>4636)
                     /locus_tag="VFV25_03085"
     CDS             complement(3267..>4636)
                     /locus_tag="VFV25_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HEY1392327.1"
                     /translation="HTLTQHLSAPSARGRDRGTAPPVISTPALLQELQQRKQALKQAA
                     STPVERATIEIVALLFQSILTEERIPATVRVWFARLQMPVLRVAVSEPDFFATIDHPA
                     RKLIDRMGACVMGFDATTRAVGDALEKEIKRVVQVVEAYPDTGRRVFQTVLTEFEKFL
                     EHYFKNENAATRKGVSLAQQVEQREALAIQYTIELRKMLNQMPVQEGVREFLFHVWAD
                     VLATSAVKSGAASEQTKQMKRVAADLIWSAGAKASREERAEVIRKLPPLLKTLREGMA
                     QANIATEKQDEHIQALNNSLAAAFTAKAATIPAERLEELTERLETLEELLPEAGEMMI
                     DEAMVLDLSGYESAELEVVGEGGSMPTPAMMTWARELQVGSWYKLDYRNRNEGVQLAW
                     QGLRKQLSLFVTPAGRGVLFQQHRLASFLQAGLLVPAEDESLTVRATRDALAKLDGDP
                     SRLLN"
BASE COUNT          711 a         1619 c         1517 g          789 t
ORIGIN      
        1 ctacggtgtg caggcgaggg tcgggatagc ggaggatgtt caatcgtgcc atggctctag
       61 cgtgcctggg accctgcgcg gcggcctgaa cgaccggctc gcactgtgga tcgcacgcaa
      121 cgaaaggccc tctcccggcc cattcccgag cggtgacaag aggtcaattt cattgcggca
      181 tcaagggttt atgggcagaa tctgtgaaac taagtgagga ttttcgctga aaggcccagg
      241 gcctgtcgcg aagcttctct atcaccacag ggcgttggga attgtcgcac cggcgccgct
      301 cggagaccct gctcatgacc ttgcgcgccc gcccagcctt cagctccgcc acccgcccca
      361 tcgcgcgcgg gcttgcggtc gccgctgccc tcggattgct gggcgccggt gcggcctggg
      421 gccagaaggg gccgaactat cccatcacgc cggcccagcg ttccaccgcc caacaggtgg
      481 ccgaggccgg cgtgccgctg tcggagctgg cggctaatgc gccggattcc tacaccgtca
      541 agcgcggcga cacgctctgg ggtatctcca gcatctacct gaagcgcccc tggcgctggc
      601 ccgagctgtg gggcatgaac atggatcaga tccgcaatcc gcatctgatc tatcccggcc
      661 aggtgctcta cctcgaaaag accggcgacc gtgcacgctt gcgcgccggg gcggcgggcg
      721 gcggcaccgt caagctgtcg ccgcgcgtgc gcagctcgga cgcctacacg gccatcgcgt
      781 cggtgccctt gcatctcatc gagcccttcc tcaacgaggc ggtgatcttc gagaccaacg
      841 agctcgagcg cgcgccgcgc atcgtcgctt cgcaggaggg ccgcgtcttc ctgggccgcg
      901 gtgacctcgc ttatgtgcga ggcgatctca acgacgagcg cagctggcgc ctgttccgca
      961 accccaagcc gctgaccgac ccgagcaccg gcgaagtgct gggctacgag gccgcctatg
     1021 tcggcacggc ccagtacctg cgccccggcg acacccgcac catcgacggc aagcccgaga
     1081 tcattcccgc caccttcgtc atcggcgaca tcaagcagga ggccggcgtc ggcgaccggc
     1141 tggcgcccgt gccggcacgc gacttcacca actacgtgcc gcatgcgcct tcgtcgccca
     1201 tcgtcggcca gatcgcttcg ctctatggcg aagcgctgtc ggccggccag aaccagatcg
     1261 tcaccctcaa caagggcgca agcgacggcc tggagcgcgg ccatgtgctg gcgctgtggc
     1321 gcgacggcag ccgtgcctac gaccccaccc agggcggcga ccgcggcgag ccgatcaagc
     1381 tgcccgacga gcgccacggc ctgctctacg tcttccgcgt cttcaaccgc atgtcctatg
     1441 cgctgatcct gagcgtcaag gacccggtgc gcgtgggcga caagttcacc cagccctgag
     1501 tcttctctct ccgcgttctc gtctgcgatt ggcccggtga tagatcgcga cgagctcgcc
     1561 gcctggctgc gtctgctcga gacgccgcag atcggtccgg tcgcggcgcg caagctgctt
     1621 gctgctttag gctcacccca ggcgatcttt gaggcgtcgg ctggcgccct gcgcgggctg
     1681 ctgggtgaaa aggaagcgca ggcgctgcaa acggcgcccg agtcgctgcc ggccttgctc
     1741 gacgcgacct ggacctggct gcaaaccgag cctggccgcc acctgctgac gctgggcgac
     1801 gcggactatc ctgccgcgct gctggagtgc gcagacccgc cgctgctgct ctacgcgatc
     1861 ggcaggctcg aaaccctgcg tcgcccggcg cttgccgtgg tcggcagtcg caatccgacg
     1921 ccccagggct tgcagaacgc cacggccttc gccgagagcc tcagccgtgc cgggctggcc
     1981 atcgtgtccg gcttggccct gggcatagac ggtgcggccc acgaaggcgc gttgcgcgca
     2041 gcgggcagca ccatcgcggt ggtcggcacc ggcctggatc gggtctatcc gaagcagcat
     2101 cgcgagctgg cgcatcgcat cggtgagcga ggactgctgc tgagcgaata cccgctcggc
     2161 atgccgccgc tggcgcccaa tttccccaag cgcaatcgca tcatctccgg ccttgcgcgc
     2221 ggcacgctcg tggtcgaggc ggcgctgcaa tcgggctcgc tgatcacggc acgcctggca
     2281 gccgagatgg ggcgcgaagt gttcgcgatt cccggctcca tccacgcgcc ccagtcgcgc
     2341 ggttgccatg ccctgatcga gcagggcgcc aagctggtcg acagcgcgca agacgtgctc
     2401 gaagagttgc ggctcgaact ggccgctgtg ccgatcgcgc catcggttcc cgccgcggct
     2461 ggcgcggcca aggatgcggt gctggatgcg ctcggctacg acccggtcaa cctcgatgcc
     2521 ctgagcgcgc gcaccggcat ggaccccgcc gctctcagcg ctcgcctgct ggaactggag
     2581 ctcgaaggcc aggtagcgcg cctgccgggc cagctgttcc agcgcctggc tcgcggctga
     2641 agggaccacg caaatccata ctccccaatc ccgttttttc acgaggccga cacacggggc
     2701 tcgggtatag tgggctcacc atgttcgacg tgctggtcta cctctacgag aactactggc
     2761 gtcccgatgc ctgccccgac cacgcacagc tcacgcgcaa gctgtcggcc gtcggcttcg
     2821 agagcgaaga gatccaggaa gccttgacct ggctggacgg cctggccagc gccgccgaat
     2881 cgcatcatgg cgatcagggc gagctgagcc tgcgccacta caccgcggcc gagcacgatc
     2941 acatgggcga ggaatccatc ggcttcatca gcttccttga gtcggccggt gtgctgcgcc
     3001 ccccgatgcg cgagatcatc gtcgaccgtg cgatggccat ctccggtggt cccgtggccc
     3061 tggaagacct gaagatcatc gtactgatgg tgttctggag cctgggcgaa gagcccgatg
     3121 cactcatcct cgacgagctc ttcgtcgatg tcgaggacag gctgattcac tgaggcgcgg
     3181 tcggcccgcg tcaaaaccaa gatttgccca ccgccttgcg caaccgcgtc agggcggcag
     3241 caccgcggcc aacttcgtcc ggggcgtcaa ttcaacagcc ggctcggatc tccatcgagc
     3301 ttggccagcg catctcgcgt cgcgcgcacg gtgagcgact cgtcttccgc cggcacgagc
     3361 aggccggcct gcaggaagga cgcgaggcgg tgctgctgga acagcacgcc gcgtcccgcc
     3421 ggcgtgacga agaggctcag ctgcttgcgc aggccttgcc aggcgagctg caccccttcg
     3481 ttgcggttgc ggtaatcgag cttgtaccag ctgcccacct gcagctcgcg agcccaggtc
     3541 atcatggccg gggtgggcat ggagccgccc tcgcccacca cttcgagctc ggcggattcg
     3601 tagcccgaga gatccagcac catggcctcg tcgatcatca tctcgccggc ctcgggcagc
     3661 agctcttcca gcgtctcgag gcgctcggtg agctcctcca gccgctcggc ggggatggtg
     3721 gcggccttgg ccgtgaaggc agcggccagc gagttgttga gcgcctggat gtgctcgtcc
     3781 tgcttctcgg tggcgatgtt ggcctgggcc atgccctcgc gcagggtctt gagcagcggc
     3841 ggcagcttgc ggatcacttc ggcgcgctcc tcgcgcgaag ccttggcgcc ggccgaccag
     3901 atcagatcgg ccgctacgcg cttcatctgc ttggtctgct cgctggccgc gcccgacttg
     3961 acggcgctgg tggccagcac gtcagcccag acgtggaaca ggaattcgcg cacgccttct
     4021 tgcaccggca tctggttgag catcttgcgc agctcgatcg tgtactggat cgccagcgcc
     4081 tcgcgctgtt cgacctgctg ggccagcgag acgcccttgc gcgtggcggc gttctcgttc
     4141 ttgaagtagt gctcgaggaa tttctcgaac tcggtcagca cggtctggaa cacgcgccgc
     4201 ccggtgtcgg gataggcctc gactacctgc accacgcgct tgatttcctt ctccagcgcg
     4261 tcgcccacgg cccgcgtggt ggcatcgaag cccatcacgc aggcgcccat gcggtcgatc
     4321 agcttgcgcg ccgggtggtc gatggtcgcg aagaagtcgg gctcgctcac cgccacgcgc
     4381 agcaccggca tctgcagccg cgcgaaccat acgcgcacgg tggccggaat gcgctcttcg
     4441 gtcaggatgc tctgaaacag cagcgcgacg atctctatcg tcgcgcgctc caccggcgtt
     4501 gacgctgcct gcttgagggc ctgcttgcgt tgctgcaatt cctgcagcag cgcgggggtg
     4561 ctgatgaccg gcggcgcggt accccggtcg cgcccacggg cggacggcgc cgacagatgc
     4621 tgcgtcagcg tgtgct
//
LOCUS       DASUCY010000158         2165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_51632, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000158 DASUCY010000000
VERSION     DASUCY010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51632"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1301
                     /locus_tag="VFV25_03090"
     CDS             <1..1301
                     /locus_tag="VFV25_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392328.1"
                     /translation="AAYWGAYTLGWLEDEARGAGRPFAADIYAISGVSGGSLGAAAFV
                     AALASEQAALQQGGGHRAASLAGWMGELLGRDDLSPVVGMALFPDLVARLSPVAIRRF
                     DRSQALELTWEQDTRRLARAAVGVDTPPWFACPLEALHDAPETCPGAKPAATPVPHLI
                     LNSASARSGRPALQASLDLQHPSAIGGRNPVFALDGLTLSGAVHNSARFPYLSPAGRV
                     LGHNGASVDYWVDGGYFENSGVWALQALLHEMSKAEGFDAGVRARLRFIVFSNDPVHA
                     DSGSWLPQPPAPPPSGALAKPVPPRTLGGGVASQDRPILLAELFAPPEGLFTARAARA
                     DAEVLRLAALLRDDWQIEPTAVIRQLQLPATYGRAPSMNWFINHRSTCVMRSMVLARD
                     ECGAKLGGAPAPLPAQIASAPAYLRYRLQRDALHAEVVGR"
     gene            complement(1305..2006)
                     /gene="yihA"
                     /locus_tag="VFV25_03095"
     CDS             complement(1305..2006)
                     /gene="yihA"
                     /locus_tag="VFV25_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEY1392329.1"
                     /translation="MSPAPEPRSIQPAEPADAADAKAALAWAHTARFLTTAAQLHQLP
                     VTELPEIAFVGRSNAGKSTAINTLTQQRQLAFASKTPGRTQHVNLFALGPRDEPDALL
                     ADLPGYGYARVERQAKLRWQEVMAQYLELRRNLAGVVQMIDSRHGYTDLDRQLLALIA
                     PRVGTGQVKLLVLLTKADKLNRKESAQAMRDAQQVLADVATDHADVGIALFSALKKSG
                     VDDAALALRSWVAAS"
BASE COUNT          291 a          748 c          775 g          351 t
ORIGIN      
        1 gggcggccta ttggggcgcc tatacgctgg gctggctgga ggacgaggcg cgcggcgcgg
       61 gacggccctt cgcggccgac atctatgcga tcagcggcgt ctcgggcggc agcttgggcg
      121 cggccgcttt tgtcgcggcg ctggcgagcg agcaggcggc attgcagcaa ggcggcgggc
      181 accgcgccgc atcgctggcc ggctggatgg gcgagctgct gggccgcgac gatctctcgc
      241 cggtggtggg catggcctta tttcccgatc tggtggcgcg gctgtcgccg gtagccatcc
      301 ggcgcttcga ccgctcgcag gcgctggagc tgacctggga gcaggacaca cgccggctgg
      361 cgcgagcagc cgtcggcgtc gacacgccgc cctggttcgc ctgcccgctg gaggccttgc
      421 acgacgcgcc cgaaacctgc ccgggcgcca agccagcagc aacgccggtg ccgcatctga
      481 tcctcaacag cgcctcggcg cgcagcggcc ggccggcgtt gcaggccagc ctggatctgc
      541 agcatcccag cgcgatcggc gggcgcaatc cggtgttcgc gcttgacggc ctcacgctca
      601 gcggcgccgt gcacaactcg gcccgctttc cctatctgag cccggccggc cgagtgctgg
      661 gccacaacgg cgccagcgtg gactactggg tcgacggcgg ctatttcgag aactcgggcg
      721 tctgggcctt gcaagccctg ctgcacgaga tgagcaaggc cgagggcttc gacgccggcg
      781 tgcgcgcccg attgcgcttc atcgtcttca gcaacgatcc cgtgcatgcg gacagcggtt
      841 cctggctgcc gcaaccgccg gccccgccgc catcaggcgc cttggccaag ccggtaccgc
      901 cgcgcacgct gggcggcggc gtggcctcgc aggaccgccc catactgctg gccgagctgt
      961 tcgccccgcc cgagggcttg ttcacggccc gtgccgcgcg cgccgatgcc gaagtgctgc
     1021 ggctggccgc cctgctgcgc gacgactggc agatcgagcc cacggccgtg atccgccagc
     1081 tgcagctgcc ggccacctat ggccgggcgc catcgatgaa ctggttcatc aaccaccgct
     1141 cgacgtgcgt gatgcgctcc atggtgttgg cgcgcgacga atgcggtgcc aagctcggcg
     1201 gtgcccccgc accgctgccc gcgcagatcg cctctgcgcc ggcctacctg cgctaccggc
     1261 tgcagcgcga tgcgctgcat gctgaggtgg tgggccgctg agattcagga agccgcgacc
     1321 cagctgcgca gcgccagcgc cgcgtcgtcc acgccggact tcttcagcgc cgaaaacagc
     1381 gcgatgccaa cgtcggcatg gtcggtggcg acatcggcca gcacctgctg ggcatcgcgc
     1441 atcgcctgcg cgctctcctt gcggttgagc ttgtcggcct tggtgagcag caccagcagc
     1501 ttgacttggc cggtgcccac gcgcggcgcg atcaaggcga gcagttggcg gtccaggtcg
     1561 gtatagccgt ggcgcgaatc gatcatctgc accacgccgg cgagattgcg acgcagctcc
     1621 aggtattgcg ccatcacttc ctgccagcgc agcttggcct ggcgctccac cctcgcatag
     1681 ccgtagccgg gcaggtcggc cagcagcgcg tcgggctcgt ccctggggcc gagcgcgaac
     1741 aggttgacgt gctgggtgcg cccgggcgtc ttggacgcga aggcgagctg gcgctgctgg
     1801 gtcagcgtgt tgatcgcggt ggacttgccg gcgttggagc ggccgacaaa ggcaatctca
     1861 ggcagctcgg tgacgggcag ctggtgcagc tgggccgccg tggtcaggaa gcgcgccgtg
     1921 tgagcccagg ccagggcggc ctttgcatcg gcagcgtctg cgggttcggc gggctgtatg
     1981 gaacggggtt cgggagctgg actcatgggg cattgtaaaa ttgcccggtt ttgcacaata
     2041 aatgggccat cgccatgaag tccttcgcct ccttgcgcgc ttctttgcta gtggctgcca
     2101 tgctggcagc cccggccgct ctgctgagcg gcaacgccca ggccgccgag tctgcggccg
     2161 cggcc
//
LOCUS       DASUCY010000159         3870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1385163, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000159 DASUCY010000000
VERSION     DASUCY010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1385163"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..1077)
                     /locus_tag="VFV25_03100"
     CDS             complement(166..1077)
                     /locus_tag="VFV25_03100"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEY1392330.1"
                     /translation="MPVYSMTGYANVAGVAPQGQENGQIPAASVAVELRSVNSRFLDL
                     AFRMPEEFRSLEPALREMLTGAFRRGKIELRLAPKSEADSAWPHPQPEQLNTLARLES
                     TVQAWLPKAVPLSVHEALQWCRSASPAPRLDEAALAAARQAVAALRESREREGERLVG
                     ILKERITHLRQLAAQAAPLLPQVVAKQQQRFLERWKEALESSGAAASLSAEALQERAL
                     NEAAAYAIRIDVAEELSRLNAHLDEISRLLAAGGELGKRLDFLIQELHREANTLGSKS
                     AALELTQISVEMKVAIEQLREQVQNIE"
     gene            1106..2074
                     /locus_tag="VFV25_03105"
     CDS             1106..2074
                     /locus_tag="VFV25_03105"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY1392331.1"
                     /translation="MSKPKPAPLPAGTVVGGYQIVKKLAAGGFGVVYLAEDPERRLIA
                     LKEYLPSSLAERSPGELTPRVKPDKLPLYRLGLKSFFEEGRSLAQISHPSVVSVLNFF
                     RENETVYMVMNYLQGDTLQDFIVTARDLKRDKVFRESTIRSLFDEILRGLRIVHQHKM
                     LHLDIKPANIFITNDNRAVLLDFGAAREVLSKEGNFIRPMYTPGFAAPEMYRRDGTLG
                     PWTDIYAIGACIYACMQGYPPNDAPQRIEKDRLGLSLSRLRNVYSDNLIEVTEWCMSL
                     DPLSRPQSVFALQKELARETERRYTKLSFSERLKLQLENLKTGAKA"
     gene            2104..3042
                     /locus_tag="VFV25_03110"
     CDS             2104..3042
                     /locus_tag="VFV25_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HEY1392332.1"
                     /translation="MRFSVYQISRKGGREKNEDRMGYCYTRDSGLFALADGMGGHPEG
                     EVASQIALQTLAALFQRDAKPVLKDPLRFLHDAIIAGHHQLIRYATEKALIDTPRTTI
                     VACLLQGNTAYWAHCGDSRLYLVRGDKLIARTRDHSYSELQETLSQVVPIGERFNRNV
                     LFTCLGSPGKPVVDTVGPLILQEGDRLLLCSDGLWGSVSDELITEQLAASAISDSVPE
                     LVEQALRNGGPKSDNVTVLAVEWEGAEDPESGKGISTQSLGEEVFASTIQASVGSGSA
                     DPGELDEAEIERSIREINEAIQRSSAKSNGTQKKSS"
     gene            3079..3810
                     /gene="rph"
                     /locus_tag="VFV25_03115"
     CDS             3079..3810
                     /gene="rph"
                     /locus_tag="VFV25_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEY1392333.1"
                     /translation="MSSFERAGGRAADALRPVRITRRYTKHAEGSVLVEFGETKVLCT
                     ASVEEKVPPHKRGSGEGWVTAEYGMLPRSTHTRSDREAARGKQSGRTQEIQRLIGRSL
                     RAVFDLAALGERSIQLDCDVIQADGGTRTAAITGAFVAAHDAVSWLLAQGKLTRSPVR
                     DFIAAVSVGIVDGVQLLDLEYSEDSACDTDMNVVMTGSGGFVEIQGTAEGATFTRAEM
                     DRLLALADSGIRSLIAAQKAALAAD"
BASE COUNT          678 a         1259 c         1280 g          653 t
ORIGIN      
        1 tcgagagccg cgcgaggggc cttgcgccgg ggcaagctac cggcagttca ccccacggaa
       61 gccgcgcgct tcagtgagac gacctgggac gccgtgatat tgactaccgt tgcgggtttg
      121 cgccgagcat cgaactgcgg gacggcgtga gacgccgtga gacgttcatt cgatgttttg
      181 cacctgctcg cgcagctgct cgatggcgac cttcatctcc accgagatct gggtgagttc
      241 cagcgcagcg gattttgagc ccagggtgtt ggcttcgcga tgcagttcct ggatcaggaa
      301 gtcaagccgc ttgccgagct cgccgccggc ggcgagcagg cggctgatct cgtccagatg
      361 ggcgttcagt cgcgacagct cttcggccac atcgatgcga attgcatagg ccgcggcctc
      421 gttcaaggcg cgctcctgca aggcctcggc agacaggctg gcggcggcgc cgctgctttc
      481 cagcgcctcc ttccagcgct ccaggaagcg ctgctgttgc ttggcgacaa cctgcggcag
      541 caatggcgca gcctgggcgg ccagctgacg caggtgggtg atgcgctcct tcaggatgcc
      601 gaccaagcgc tcgccctcgc gctcgcggga ttcgcgcagc gctgcgaccg cctggcgagc
      661 cgcagccagg gcggcctcgt ccagccgcgg cgccggactg gcgctgcggc accattgcaa
      721 ggcctcgtgc acgctcagcg gcacggcctt ggggagccaa gcctggaccg tgctttccag
      781 ccgcgccaag gtgttgagct gctcgggctg cggatgaggc caggcgctgt ccgcctcgct
      841 cttgggggcc aggcgcaatt cgatcttgcc gcgccgaaag gctcccgtga gcatctcgcg
      901 cagggcgggc tccagcgagc ggaactcctc gggcatgcga aaggcgagat cgagaaagcg
      961 gctgttgacc gagcgcagtt cgactgccac gctggcagcg ggaatttggc cgttttcctg
     1021 gccttgcggg gctacgccgg ccacgttggc atagcctgtc atgctgtaaa ctggcatcgc
     1081 acttatcgca ttgcaaaatt cgattatgtc aaagcccaag cccgccccat tgcccgcggg
     1141 gaccgtggtc ggtgggtacc agatcgtcaa gaagctggct gcaggcggtt ttggcgtggt
     1201 ctatctggcc gaggatcccg agcggcgcct gatcgccctc aaggaatacc tgccgtcttc
     1261 gctggccgag cgctcgcccg gcgagctcac gccgcgcgtc aagcccgaca agctgccgct
     1321 ttaccgcctt ggcctcaaga gcttcttcga ggaagggcgc tcgcttgcgc agatttcgca
     1381 cccgagcgtg gtctcggtgc tcaacttctt ccgcgagaac gagaccgtct acatggtgat
     1441 gaactacctg cagggagaca ccctgcagga cttcatcgtc acggcgcgcg atctcaagcg
     1501 cgacaaggtg ttccgcgagt cgacgatacg ctcgctgttt gacgagatcc tgcgcggctt
     1561 gcgcatcgtg caccagcaca agatgctgca cctagacatc aagccggcca atatcttcat
     1621 caccaacgac aaccgggccg tgctgctcga tttcggcgcg gcgcgcgagg tgctgagcaa
     1681 ggaaggcaac ttcatccgcc cgatgtacac gcccggcttc gcggcgcccg agatgtaccg
     1741 ccgcgacggc acgctgggtc cctggaccga catctacgca atcggcgcct gcatctatgc
     1801 ctgcatgcag ggctatccgc ccaacgatgc gccgcagcgc atcgagaagg accgcctggg
     1861 cctgtcgctg tcgcgcctgc gcaatgtcta ttcggacaac ctgatcgagg tcaccgaatg
     1921 gtgcatgtcg ctcgacccgc tgtcgcgacc gcaaagcgtg ttcgcgctgc agaaggaact
     1981 ggcgcgcgag accgagcgcc gctacaccaa gctcagcttc agcgaaaggc tgaagctgca
     2041 actcgaaaac ctgaagacgg gcgccaaggc ctgatcctgc agccaagccg ctgaaagcca
     2101 ttcatgaggt tttccgttta ccagatcagc cgtaagggcg gccgcgagaa gaacgaagac
     2161 cgcatgggct attgctacac gcgggactcg ggcctgtttg cgctggccga cggcatgggt
     2221 ggccatcccg aaggcgaggt ggcctcgcag atcgcgctgc agacactggc cgcgctgttc
     2281 cagcgcgacg ccaagccggt gctgaaggat ccgctgcgct tcctgcacga cgccatcatc
     2341 gccggtcacc atcagctgat ccgctacgcg accgagaagg cgctgatcga cacgccgcgc
     2401 accaccatcg tggcctgcct gctgcagggc aacaccgcct actgggcgca ttgcggcgat
     2461 tcgcgcctgt atctcgtgcg cggcgacaag ttgatcgccc gcacccgtga ccactcctat
     2521 tccgagctgc aggagacgct gagccaggtc gtgccgatcg gcgagcgttt caatcgcaac
     2581 gtgctcttca cctgcctggg cagtcccggc aagccggtag tcgacaccgt cgggccgctg
     2641 atcttgcagg agggtgatcg tctgctgctg tgctcggatg ggctgtgggg cagtgtcagc
     2701 gacgagctca tcaccgagca gctggcggcc agcgcgatct cggactccgt gccggagctg
     2761 gtggagcagg cgctgcgcaa cggcggcccc aagagcgata acgtcacggt gctggccgtc
     2821 gagtgggagg gcgccgagga tccggagtcc ggcaagggca tctccacgca gtcgctcggc
     2881 gaggaggtct ttgcgtcgac aatccaggcc agcgtgggca gtggcagcgc cgatcccggc
     2941 gaactcgacg aggccgagat cgagcgctcg atccgcgaga tcaacgaggc catccaacgc
     3001 tcttctgcca agagcaacgg cacgcagaag aagtcttcct gaggcattcc cgcctctcac
     3061 tcaatcccaa ggaaagtcat gagcagtttc gagcgcgcgg gcggccgcgc cgccgacgca
     3121 ttgcgtcccg tgcgcattac gcgccgctac accaagcatg ccgagggctc ggtgctggtg
     3181 gagttcggcg agaccaaggt gctgtgtacc gcgtcggtgg aggagaaggt gccgccgcac
     3241 aagaggggca gcggtgaggg ctgggtgacg gccgaatacg gcatgctgcc gcgctccacg
     3301 cacacgcgca gcgaccgcga ggcggcacgc ggcaagcaga gcggccgcac ccaggagatc
     3361 cagcgcctca tcgggcgttc gctgcgcgcc gtcttcgatc ttgcggccct gggcgagcgc
     3421 agcatccagc tcgactgcga cgtgattcag gccgacggtg gtacgcgcac tgcggccatc
     3481 accggtgcct ttgtcgcggc gcatgatgcg gtgagttggc tgcttgccca aggcaagctc
     3541 accaggtcgc cagttcgcga cttcatcgcg gcagtgtcgg tcggcatcgt cgacggcgtg
     3601 cagctgctgg atctcgaata cagcgaagac tccgcctgcg acaccgacat gaacgtcgtg
     3661 atgacgggct cgggcggctt tgtcgagatc cagggcacgg ccgagggcgc caccttcacg
     3721 cgcgccgaga tggatcgcct gctcgcgctg gccgacagcg gcatacgcag cctgatcgct
     3781 gcgcagaagg ccgcgctggc agccgattga ggggtcgggc atgaggctgg tgctcgcctc
     3841 caacaacgcg aagaagcttg ccgaactgca
//
LOCUS       DASUCY010000160         2020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1288651, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000160 DASUCY010000000
VERSION     DASUCY010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1288651"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="VFV25_03120"
     CDS             complement(<1..702)
                     /locus_tag="VFV25_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2817 domain-containing protein"
                     /protein_id="HEY1392334.1"
                     /translation="MAAAAAYFSQSYAEARGKFLAAVDAAGLDVEPHLHPLRGRDGEE
                     LAMDIVREGPHSCEHLLIISSACHGVEGFCGSGIQTALLSAPTWRAAVREAGLAVLYV
                     HALNPYGFSWLRRVTQENVDLNRNFHDFSKPLPANPAYDEIAHLLVPPTWPPAQQITA
                     EMQRYLAERGVKALQAAISGGQHEHPEGLFYGGINPTWSNLALRHVLREQGAGVKHLG
                     WIDLHTGLGPSGHGER"
     gene            complement(707..1903)
                     /locus_tag="VFV25_03125"
     CDS             complement(707..1903)
                     /locus_tag="VFV25_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HEY1392335.1"
                     /translation="MQLIESILADAQSIKAVRRDIHAHPELCFEEVRTSDLIAKQLTD
                     WGIPVHRGLGKTGVVGIIKNGSSSRAVGLRADIDALPMTEHNHFAHASQHPGKMHACG
                     HDGHTAMLLAAAKHFSRHRNFDGTVYLIFQPAEEGGGGAREMIKDGIFDRFPMEAVFG
                     AHNWPGMKVGQFAIKSGPCFASSNEFKITIRGKGAHAAMPHLGLDPVPVACQMVQAFQ
                     TIISRNKRPIDTGVISVTMIHTGEATNVVPESCVIEGTVRTFTTEVLDLIERRMKTIA
                     EATCAAFEVGCEFEFMRNYPPTVNHPDETQFAREAMADVVGADNVLEFEPTMGAEDFS
                     YFLQVKPGAYFLIGNGDGTHREGGHGMGPCMLHNPSYDFNDDLIPLGATLWVRLAEKW
                     LATPRG"
BASE COUNT          295 a          661 c          709 g          355 t
ORIGIN      
        1 gcgctcgcca tggccgctgg ggccgaggcc ggtgtgcagg tcgatccagc ccaggtgctt
       61 cacaccggcg ccctgctcgc gcagcacatg gcgcagcgcg agattgctcc aggtcgggtt
      121 gatgccgccg tagaacaggc cttcgggatg ctcgtgctgc ccgcccgaga tggccgcctg
      181 cagcgccttg acgccgcgct cggcgaggta gcgctgcatt tcggccgtga tctgctgcgc
      241 tggcggccag gtcggcggca ccagcaggtg agcgatctcg tcataggccg ggttggccgg
      301 caggggcttg gagaagtcgt ggaaattgcg gttcaggtcc acattctcct gggtcacgcg
      361 gcgcagccac gagaagccgt aggggttgag cgcgtggacg tacagcacgg ctaggccggc
      421 ctcgcgcact gcggcacgcc acgtgggggc gctcagcagc gcggtttgta tgcccgagcc
      481 gcagaatccc tcgacgccat ggcaggcgct gctgatgatg agcaagtgtt cgcagctgtg
      541 cgggccctcc cgcacgatgt ccatcgccag ctcctcgccg tcgcgcccgc gcaaggggtg
      601 tagatgcggc tcgacatcga gcccggccgc gtcgacggcg gccaggaact tgccgcgcgc
      661 ttcggcatag ctctgggaaa aatatgcagc cgccgcagcc atgggctcag cccctggggg
      721 tggcgagcca cttctcggcc aggcgcaccc acagcgtcgc gcccagcggg atcaggtcgt
      781 cgttgaagtc gtagctgggg ttgtgcagca tgcagggccc catcccgtgc ccgccttcgc
      841 gatgggtgcc gtcgccgttg ccgatcagga aataggcgcc gggcttgacc tgcaggaagt
      901 agctgaagtc ctcggcgccc atcgtcggct cgaactccag cacgttgtcc gcgcccacca
      961 catcggccat cgcctcgcgg gcgaactgcg tctcgtccgg gtgattgacc gtgggtgggt
     1021 agttgcgcat gaactcgaac tcgcagccga cctcgaaggc ggcgcaggtc gcctcggcga
     1081 tcgtcttcat gcggcgctcg atcaggtcca gcacctcggt cgtgaaggtg cgcaccgtgc
     1141 cctcgatgac gcagctctcg ggcaccacat tggtcgcctc gccggtgtgg atcatggtca
     1201 ccgagatcac gccggtgtcg atcggccgct tgttgcggct gatgatggtc tggaaggcct
     1261 gcaccatctg gcaggccacc ggcaccgggt ccaggcccag gtgcggcatc gcggcgtgcg
     1321 cgcccttgcc gcgtatcgtg atcttgaact cgttgctgga ggcgaagcag gggccgctct
     1381 tgatcgcgaa ctggcccacc ttcatgcccg gccagttgtg ggcgccgaac acggcctcca
     1441 tcgggaagcg gtcgaagatg ccgtccttga tcatctcgcg ggcgccgccg ccgccttcct
     1501 ctgcgggctg gaagatcagg tagaccgtgc cgtcgaaatt gcggtgccgg ctgaaatgct
     1561 tggccgcggc cagcagcatt gcagtgtggc cgtcgtggcc gcaggcgtgc atcttgccgg
     1621 ggtgctggct ggcatgggcg aaatggttgt gctcggtcat cggcagcgcg tcgatgtcgg
     1681 cccgcagacc cacggcgcgg gaagagctgc cattcttgat gatgcccacc acgccggtct
     1741 tgcccaggcc gcggtggacg gggatgcccc agtcggtcag ctgcttggcg atcaggtccg
     1801 aggtgcggac ctcctcgaag cacagctcgg gatgggcatg gatgtcgcgg cgcacggcct
     1861 tgatgctctg cgcatcggcc aggatggatt cgatcaattg catgacgagg gctcccggat
     1921 ccggagcgcg tggccccgga gaagccatct tagccgttcc gccgcttccg gtaccccctg
     1981 cgtgagccgg ccaagtgggc gcccgcggat ccggcttgcc
//
LOCUS       DASUCY010000161         6110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1433349, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000161 DASUCY010000000
VERSION     DASUCY010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 6110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6110
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1433349"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VFV25_03130"
     CDS             <1..204
                     /locus_tag="VFV25_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HEY1392336.1"
                     /translation="KAIKAAKVVGFADLGMEAIYEFDVVDMPVTVAVDAGGTSAHITG
                     PADWQQHIATGQFKGIELAAAKA"
     gene            348..725
                     /locus_tag="VFV25_03135"
     CDS             348..725
                     /locus_tag="VFV25_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY1392337.1"
                     /translation="MSTPETVVQRQLDAYNAKDLSAWLATYAPDAEQFELHGPRLTAG
                     HAQMQERMALRFTEPDLHAELLSRVVMGAVVVDHERVTRNFAEGKGTIEMLCVYEVAN
                     GVIAKASFALGAKRVLGRAQSAI"
     gene            797..1210
                     /locus_tag="VFV25_03140"
     CDS             797..1210
                     /locus_tag="VFV25_03140"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY1392338.1"
                     /translation="MHPASPQSSRVAACSCGQLQAHVVGEPLRVSVCHCLACQRRTGS
                     MFGAQARFPREAVTVVGASTEYVRTGDEGTKSTFRFCPKCGATVYYTMEGLAEAVAIP
                     VGAFAEPSFPAPSFSVYEERMHSWVSMPSGIERMA"
     gene            complement(1230..2507)
                     /locus_tag="VFV25_03145"
     CDS             complement(1230..2507)
                     /locus_tag="VFV25_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEY1392339.1"
                     /translation="MTHHPINREELRSLVESLLKPTAFTELLVLAVCLALAWTVARLL
                     RGTQARPRSIWFGYRIFDGVLFPVLSLVFAFVARDLLSGMVPIAVFRLALPILMSLLV
                     IRLFVRVMTAAFPNAQVMRVVERSVSWVVWIGVVLWFTGVLPTVLEELDGITWKMGAS
                     TISLRNVVEGSLSVVVALVLVLWMSSAIEARLLKDATHENLSVRKITANAVRALLVFV
                     GMLFALSAAGIDLTALSVLGGAIGVGLGFGLQKLAANYVSGFVVLAERSLRIGDLIKV
                     DNFEGRITDIKTRYTVIRSAGGRESIVPNEMLITQRVENASLTDTKVLMSTVVQVAYG
                     TDVRDISAKLQAAATRVPRVMGDPGPSVHLTAFAPDGLELTVWFWIPDPENGQSNVRS
                     AVNLAILETLNAEGIDIPYPQRVVRQIVDAVRS"
     gene            complement(2558..3496)
                     /locus_tag="VFV25_03150"
     CDS             complement(2558..3496)
                     /locus_tag="VFV25_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEY1392340.1"
                     /translation="MSTAYYTHPDCRAHDMGRGHPECPQRLAAIEDHLIATGLDIALT
                     HREAPKVEPGELALAHEQGYVNQLGEFMRAVRESGESRHIDPDTVVCPASWDAALRAA
                     GAAVAATDAVLAGEVENAFCAVRPPGHHATRREAMGFCFFNNVAVAARHALDVRGLQR
                     VAIVDFDVHHGNGTEDIIAGDDRVLMVSFFQHPLYPGCGTEPMGDNMLNLPVPPYTKG
                     MAIRELIEALWLPRLNEFKPQMIFVSAGFDAHREDDLGQLGLVEADYDWITRRIKEVA
                     ERHARGRIVSCLEGGYALGALARSVAAHLRVLAGVG"
     gene            3547..4467
                     /locus_tag="VFV25_03155"
     CDS             3547..4467
                     /locus_tag="VFV25_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY1392341.1"
                     /translation="MSLHTRLQDLIGQISTIIVGKRPQIEDCMACLLAGGHLLIEDVP
                     GVGKTTLAHALAVSLGLKFSRVQFTADLMPSDLIGVSVYERGKEAFVFHGGPVFAHVL
                     LADEINRAGPKTQSALLEAMEEHQVTVDNETRALPEPFFVIATQNPTDQLGTYPLPES
                     QLDRFLMCITLGYPDRASERALLAGRDRREAIHELRPVMTPAELHAVQQAVLQIHASE
                     ALLDYLQALIAATRSGHWFVEGLSPRAGIAVLRAAKARALLDQRDYVAPDDIQAVLPQ
                     TVAHRLVPVAGAGRGRLEQVRAMIAATPLS"
     gene            4588..5553
                     /locus_tag="VFV25_03160"
     CDS             4588..5553
                     /locus_tag="VFV25_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY1392342.1"
                     /translation="MMRQRWRAWWQARSPRSDTLALEQRNIYIVPTRAGLMFCLTLLV
                     LLVASINYQLNLGYLLTFLLAGAGVVSMHVTHSTLRGLKLHLRPVVPVFAGEALTLEV
                     VLGSSAARPRYGIGLRVDAPGRAAAWSWSDVAAGGQASTQVSYVAPRRGLHGLPTLQI
                     ETRFPLGLFRAWSFLRPAAQALVYPAPEQPAPAMPAARAIPGGPSQARSRDGGEIEGI
                     RAYRRGDPLKLVMWKKAARALETGGELASRDTASSAHYELWLDWQLTGTGLTAEERLS
                     RLAAWVLAAHRAGVLYGLRLPGRELAPADGQAHRRACLEALALWA"
     gene            5556..>6110
                     /locus_tag="VFV25_03165"
     CDS             5556..>6110
                     /locus_tag="VFV25_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HEY1392343.1"
                     /translation="MAELASLLGARMRHLPRETRDTLFLLAVIAWTVLPHVGHIPLWC
                     SLLAGVVLLWRTQLALGSAPLPGRWMPAVVLLVAAVLTLWSYRTLLGKEAGVTLIVVL
                     AALKTLELRARRDAFVVFFLGFFLVLTQFLYSQSLLTAASMLVAVWGLLTGLVLAHMP
                     VGQPALRQAAALAARSALLGAPLMA"
BASE COUNT          958 a         2085 c         2099 g          968 t
ORIGIN      
        1 aaggccatca aggcggccaa ggtcgtcggc ttcgccgacc tcggcatgga agcgatctac
       61 gagttcgacg tggtcgacat gccggtcacg gtggccgtgg atgccggcgg caccagcgcc
      121 cacatcacgg gcccggccga ttggcaacag catatcgcca cgggccagtt caagggcatc
      181 gagctggcgg cggccaaggc ttagcaaggc atgccaccgc tctcgcttct cgtgagcacc
      241 gtcacaacca ggccgcctgc gggcggcctt ttcttctttg caggcaggcc tgcaccgtac
      301 gatgtcgtga gcggcgagcg gcacgggcgt cggctatgct gagacgcatg agcactccgg
      361 agacagtcgt tcaacgccag ctcgacgcct acaacgccaa ggatctatcg gcctggcttg
      421 ccacttacgc tcccgatgcg gagcaattcg agctgcatgg gccccggctt actgcgggcc
      481 atgcccaaat gcaggagcgc atggcgctca ggttcaccga acccgatctc cacgctgaac
      541 tgctgtcgcg ggtggtcatg ggcgctgtcg tcgtcgatca tgagcgcgtt actcgcaact
      601 ttgccgaggg caagggcacg atcgaaatgc tctgcgtgta cgaggtggcc aacggtgtca
      661 tcgccaaggc atcatttgca ctcggagcga agcgcgtcct tggccgtgcg caaagtgcca
      721 tctaaccatc aatcgagcga cagccacagc tacgctgttt ggccgccgct tgtgtccaac
      781 attaggaggc gcagaaatgc atcccgcatc tccccagtct tctcgagttg ccgcctgtag
      841 ctgtgggcag ttgcaagcgc atgtcgtcgg cgagcccttg cgcgtgtctg tgtgccactg
      901 cctggcgtgt cagcgccgca ctggaagcat gttcggtgcg caagcgcggt ttccacgcga
      961 ggcggtcacg gtcgttggtg cgagcaccga gtacgttcgc accggagacg aaggaacgaa
     1021 aagcacattc aggttctgcc cgaagtgcgg tgccaccgtg tactacacga tggaggggct
     1081 ggcggaggcc gttgcgatac cggtgggtgc attcgccgag ccgtcgtttc cggcgccgag
     1141 cttctcggtc tacgaagagc gcatgcactc gtgggtcagc atgccaagtg gcatagagag
     1201 aatggcatga gaccaacagc gccttggcat cagctccgca ccgcatccac gatctgccgc
     1261 accacgcgct gcggataggg gatgtcgatg ccctcggcat tcagcgtctc gaggatcgct
     1321 aggttgactg ccgagcgtac attggactgg ccgttctccg gatcggggat ccagaaccag
     1381 accgtgagct ccaggccgtc gggcgcgaag gcggtgagat gcacggaggg gccggggtcg
     1441 cccatcacgc gcgggacccg cgtcgcggcc gcctgcagct tggcgctgat gtcgcgcacg
     1501 tcggtgccat aggccacctg cacgacggtg gacatcagca ccttcgtatc ggtgagcgag
     1561 gcgttctcga cgcgctgggt gatcagcatc tcgttgggca cgatggactc gcgcccgccg
     1621 gccgagcgga tcaccgtgta gcgcgtcttg atgtcggtga tgcggccctc gaagttgtcg
     1681 accttgatca ggtcgccgat acgcaaggac ctttcggcca gcaccacgaa gccgctcacg
     1741 tagttggcgg ccagcttctg caggccaaag cccaggccta cgcctatcgc gccgcccagc
     1801 acgctcaagg cggtgaggtc gatgccggcg gccgacagcg cgaacagcat ccccacgaag
     1861 acgagcagcg cgcggaccgc gttggcggtg atcttgcgca ccgagaggtt ctcgtgcgtc
     1921 gcgtccttga gcagcctggc ctcgatggcc gaagacatcc acagcaccag caccagcgcg
     1981 acgacgacgc taagcgaacc ctcgaccacg ttgcgcagcg agatggtcga ggcccccatc
     2041 ttccaggtga tgccgtccag ctcctccagc accgtgggca gcacgccggt gaaccacagc
     2101 accacgccga tccacaccac ccaggacacg ctgcgctcca cgacgcgcat gacctgggca
     2161 ttggggaagg cggccgtcat cacccgcacg aagaggcgga tgacgagcag ggacatcaag
     2221 atgggcagcg ccagcctgaa cacggcaatc ggcaccatgc cggacaggag gtcgcgcgcc
     2281 acgaaggcga acaccagcga cagcaccggg aacagcacgc cgtcgaagat gcggtaaccg
     2341 aaccagatcg agcgcggcct tgcctgggtg ccgcgcagga ggcgcgccac ggtccaggcc
     2401 agggccaggc agacggccag caccagcagc tcggtgaagg cggtgggttt gagcagcgat
     2461 tcgacgaggc ttcgaagctc ctcgcggttg atcgggtgat gggtcatgaa tattgtcggg
     2521 acaagagaag cttgctcggg tcacgcgggc ctaggcttca acccacgccg gccagcacgc
     2581 gcaaatgggc ggccacgctg cgcgccagcg cgccgagggc gtagccgcct tcgaggcagg
     2641 acacgatgcg gccccgtgca tggcgctcgg ccacctcctt gatgcgccgc gtgatccagt
     2701 cgtagtcggc ctcgaccagg ccgagctggc ccaggtcgtc ttcgcgatgg gcatcgaagc
     2761 cggccgagac gaagatcatc tgcggcttga actcgttgag ccgcggcagc cacagcgcct
     2821 cgatcagctc gcgtatcgcc atgcccttgg tatagggcgg caccggcagg ttgagcatgt
     2881 tgtcgcccat gggctcggtg ccgcagcccg ggtacagcgg atgctggaag aaactcacca
     2941 tcagcacgcg atcgtcgccg gcgatgatgt cctcggtgcc gttgccatgg tgcacgtcga
     3001 agtcgacgat ggccacgcgc tgcaggccgc gcacatccaa cgcatggcgc gccgcgacgg
     3061 cgacgttgtt gaagaagcag aaacccatgg cctcgcgccg cgtcgcgtgg tggcccggcg
     3121 ggcgcacggc gcagaaggcg ttctccacct cgccggccag caccgcatcg gtggccgcca
     3181 ccgcggcgcc ggccgcgcgc agcgcggcat cccagctggc agggcagacg acggtgtcgg
     3241 gatcgatgtg gcgcgactcg ccggactcgc gcaccgcgcg catgaattcg cccagctggt
     3301 tgacgtagcc ctgctcgtgc gccagcgcca gctcacccgg ctcgaccttg ggggcctcgc
     3361 ggtgcgtcag cgcgatgtcc agcccggtgg cgatcagatg gtcctcgatg gcggccaggc
     3421 gctgcgggca ctcgggatgg ccgcggccca tgtcgtgggc ccggcagtcg ggatgggtgt
     3481 agtacgcggt agacatccgg gcgggaagac tctggaagat gctttttatg agctaaggtg
     3541 cgggccatga gcctgcacac ccgactccaa gacctcatcg gccagattag cacgatcatc
     3601 gtcggcaagc gcccgcagat cgaggactgc atggcctgcc tgctggccgg cggccacctg
     3661 ctgatcgaag acgtgccggg cgtgggcaag accacgctgg cccatgccct ggccgtgtcg
     3721 ctgggcctga agttctcgcg cgtgcagttc accgccgacc tgatgcccag cgacctgatc
     3781 ggcgtcagcg tatacgagcg cggcaaggag gccttcgtct tccacggcgg cccggtgttc
     3841 gcccatgtgc tgctggccga cgagatcaac cgcgccggcc ccaagacgca gagtgccctg
     3901 ctggaggcca tggaggagca ccaagtcacg gtggacaacg agacgcgcgc cctgcccgag
     3961 cccttcttcg tcattgcgac gcagaacccg accgaccagc tcggcaccta tccgctgccc
     4021 gagagccagc tcgaccgctt cctgatgtgc atcacgctgg gctacccgga ccgcgcctcc
     4081 gagcgcgccc tgctggccgg gcgcgatcgc cgcgaggcca ttcacgaact gaggccggtg
     4141 atgacgccgg ccgagctgca cgcggtgcag caggccgtgc tgcaaatcca cgcctcggag
     4201 gcgctgctgg actatctgca ggccctgatc gcggcgacgc gctccgggca ctggttcgtc
     4261 gagggcctga gcccgcgcgc cggcatcgcc gtgctgcgtg ccgccaaggc gcgcgccctg
     4321 ctcgaccagc gcgactacgt ggcgccggac gacatccagg ccgtgctgcc gcagaccgtg
     4381 gcgcatcgcc tggtccccgt ggccggcgcc ggccgcgggc ggctggagca ggtccgcgcg
     4441 atgatcgcgg cgacgccgct ttcctgaacg ttgggcatga ggcatccagt gtcaacgtcc
     4501 ttcgcgggcc gagcgccggg ccgctcccaa gccggcccgc atcccctcgg gggatcggcc
     4561 gacgtacccg tcggacgagg ggttgtcatg atgcgccagc gctggcgcgc ctggtggcag
     4621 gcgcgctcgc cgcgcagcga cacgctggcg ctggagcagc gcaacatcta catcgtgccc
     4681 acgcgcgccg ggctgatgtt ctgccttacg ctgctcgtgc tgctggtcgc ctcgatcaac
     4741 taccagctca acctcggcta tctgctgacc ttcctgctgg ccggtgccgg cgtcgtgtcg
     4801 atgcatgtca cgcactcgac gctgcgcggc ctcaagctcc acctgcggcc cgtggtgccg
     4861 gtgttcgctg gcgaggcgct gacgctggag gtcgtgctcg gcagcagcgc cgcgcggccg
     4921 cgctacggca tcggcctgag ggtggacgcg ccaggccgcg ccgccgcctg gagctggagc
     4981 gatgtggccg cgggcggcca ggccagcacg caggtgagct atgtggcgcc gcggcgcggc
     5041 ctgcatggcc tgcccacctt gcagatcgag acgcgcttcc cgctaggcct gttccgcgcc
     5101 tggagcttcc tgcgtccggc cgcccaggcc ctggtctatc cggcccccga gcagcccgcc
     5161 ccggcgatgc cggcagcgcg tgccataccc ggcggcccct cgcaggcgcg cagccgcgac
     5221 ggcggcgaga tcgaaggcat acgcgcctac cggcgcggcg acccgctcaa gctcgtgatg
     5281 tggaagaagg cggcgcgcgc gctggaaacc ggcggcgagc tcgcgagccg cgacacggct
     5341 tcgtcggcgc attacgagct gtggctggac tggcagctca ccggcaccgg cttgaccgcc
     5401 gaggagcgcc tgtcgcggct ggcggcctgg gtgctggcgg cgcaccgggc cggcgtgctc
     5461 tacggcctgc gcctgcccgg gcgggagctg gcgcccgccg atggccaagc gcaccggcgg
     5521 gcctgcctgg aagccctggc gctttgggct tgagcatggc cgagctcgcc tccctgctcg
     5581 gcgcgcgcat gcgccacctg ccgcgcgaga cccgcgacac gctcttcctg ctggccgtca
     5641 tcgcgtggac ggtgctgccg catgtgggcc atatcccgct gtggtgcagc ctgctggccg
     5701 gtgtggtcct gctatggcgc acccagctgg cgctcggcag cgcgccgctg cccggccgct
     5761 ggatgccggc cgtcgtgctg ctggtcgcgg cagtcttgac gctgtggagc tatcgcaccc
     5821 tgctcggcaa ggaggccggg gtgacgctca tcgtggtgct cgccgcgctc aagacactgg
     5881 agctgcgcgc gcggcgcgac gctttcgtcg tcttcttcct cggcttcttc ctggtgctga
     5941 cgcagttcct gtattcacag tccctgctca cagcggcgtc gatgctggtg gccgtatggg
     6001 gcttgctgac tgggctggtg ctggcccata tgccggtcgg ccagccggcg ctgcgccagg
     6061 cggcggcgct ggcggcacgc tcggccctgc tgggggcgcc gctgatggcc
//
LOCUS       DASUCY010000162         2213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_855062, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000162 DASUCY010000000
VERSION     DASUCY010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855062"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1331)
                     /locus_tag="VFV25_03170"
     CDS             complement(<1..1331)
                     /locus_tag="VFV25_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctA"
                     /protein_id="HEY1392344.1"
                     /translation="MSRFLRSLFGQVVLALILGVIIGVAAPEFSVKLKPLGDGFIKLI
                     QMIIPLLVFCVVVHGIAGAGDLKRVGRVGVKSLVYFEVVTTIALGLGLLLAFMFQPGA
                     GMNVDPRTLDASAMSAYAENANKLTGGGTVGFLMKLIPTTVGDAFARGDVLQVLLFAV
                     LFGCALSLLGARGRQVAGFVDQLSHVLFKVMGIIIKLAPLGVLGAIAFTVGKYGIGSL
                     KQLGMLVALFYVAVVIFVVVVLGAILRVAGFNIFKLLNYLREELMVVLATTSSDSVLP
                     QIMSKLKRMGIRDSTVGLVIPTGYSFNLDAFSIYITLAAVFIAQATNTPVSMTDLLTI
                     LAISLVTSKGAHGVPGSAIVVLAATLHAIPAIPAIGLVLVLSVDWFMGIARALGNLIG
                     NCVATVAIAAWEGDIDRERAHAVLDGRAVPTTEDDLDIEVSRLPAGALSEA"
     gene            complement(1640..>2213)
                     /locus_tag="VFV25_03175"
     CDS             complement(1640..>2213)
                     /locus_tag="VFV25_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014615644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEY1392345.1"
                     /translation="EAPVPTRAEASDVATAIYDGADAVMLSAESASGKHPIEAVDMMS
                     RIIQQVEADPLYRQLIDASHTSARPGGDVAEAVCCAMRRAVALLHAAAIVCYTSSGHT
                     SLRAARERPESPILSLTPSLAAARRLALAWGVHSVHTEDVADVRQMSEIACEISRREG
                     FAQAGQMIVAIAGVPFGTPGTTNLMRIATV"
BASE COUNT          424 a          732 c          741 g          316 t
ORIGIN      
        1 caggcctcgc tcagcgcacc cgcaggcagc cggctgacct cgatatccag atcatcctcg
       61 gtggtcggta ccgcacgccc gtcgagcacg gcatgggcgc gctcgcggtc gatgtcgccc
      121 tcccaggccg cgatcgccac ggtggccacg caattgccga tcaagttgcc caatgcgcgc
      181 gcgatgccca tgaaccagtc gaccgacagc accagcacga ggccgatcgc gggaatcgcg
      241 gggatcgcat gcagcgtcgc ggccagcacg acgatggccg agcccggcac cccatgcgcg
      301 cccttggagg tgaccagcga gatggcgagg atggtcagca aatcggtcat cgacaccggc
      361 gtgttggtgg cctgagcgat gaacacggcc gccagcgtga tgtagatcga gaacgcgtcg
      421 aggttgaagg aatagccggt gggaatgacc aggcccaccg tcgaatcgcg gatgcccatg
      481 cgcttgagct tggacatgat ctgcggcagc acgctgtccg acgacgtggt cgccagcacc
      541 accatcagct cctcgcgcag atagttgagc agcttgaaga tgttgaagcc ggccacgcgc
      601 aggatcgcgc ctagcaccac gacgacaaag atcacgaccg cgacatagaa cagcgccacc
      661 agcatgccca gctgcttgag cgagccgatg ccgtacttgc cgactgtgaa ggcgatggcg
      721 ccgagcacgc ccagcggcgc cagcttgatg atgatgccca tgaccttgaa cagcacgtgc
      781 gacagctggt ccacgaagcc ggccacctgg cgcccgcgcg cgcccagcag cgacagcgcg
      841 cagccgaaca gcacggcgaa cagcagcact tgcagcacgt cgcccctggc gaaggcgtcg
      901 ccgacggtgg tcgggatcag cttcatcagg aagccgaccg tgccgccgcc cgtcagcttg
      961 ttcgcgttct cggcgtaggc gctcatcgcg ctggcgtcca gcgtgcgcgg atcgacgttc
     1021 atgccggcgc cgggctgaaa catgaaggcg agcagcaggc caagccccag cgcgattgtc
     1081 gtcaccacct cgaaatagac cagcgacttg acgcccaccc gcccgacgcg cttcaggtcg
     1141 ccggcgcccg cgatgccatg caccacgacg cagaagacca gcagcggaat gatcatctgg
     1201 atcagcttga tgaagccgtc gcccagcggc ttgagcttga cggagaactc gggcgcggcg
     1261 acgccgatga tgacgccgag gatcagcgcc agcacgacct ggccgaataa agaacgcagg
     1321 aaacgggaca tggtctctcc taggaaggaa gcggggctgg ggctagctcg ggtcattgcc
     1381 cacaaacttg catacaagca atgtaggcaa gcaatgtcga catcccatgc gtggataccc
     1441 taaacattca tgcatgggct gcaaaactgc ctgaacgcgg aagcagcggc gtggagggtg
     1501 cagcccttcg ccgatgtgct ttgaggcaca gcaaggcgcg tgccaagcac gctcagattt
     1561 ggatgcctcg gtgaagcgcg gcgggcaccg cttcgcccgc atcagcgcac cgatgatccg
     1621 cggccagctg ccgcgcagtt cagaccgtcg cgatgcgcat caggttggtc gtgcccggtg
     1681 tgccgaaggg cacaccagcg atggcgacga tcatctggcc ggcctgggcg aagccctcgc
     1741 ggcgcgagat ttcgcaggcg atctcgctca tctggcgcac gtcggccacg tcctcggtgt
     1801 gcaccgaatg gacgccccag gccagtgcaa gccggcgcgc agccgcgagg ctgggcgtga
     1861 ggctcaggat gggcgactcc ggccgctcgc gggcggcgcg caggctggtg tggcccgagc
     1921 tcgtgtagca gacgatggcc gcggcgtgca gcagggccac ggcgcgccgc atcgcgcagc
     1981 acacggcctc ggccacgtcg ccgccggggc gggccgaggt atgcgaagcg tcgatcagct
     2041 ggcgataaag cggatccgcc tcgacctgct ggatgatgcg gctcatcatg tccaccgctt
     2101 cgatcgggtg cttgcccgag gccgattcgg ccgaaagcat caccgcgtcg gcgccgtcgt
     2161 agatggcggt tgcgacatcg gaggcctcgg cgcgcgtcgg caccggggcc tcg
//
LOCUS       DASUCY010000163         4664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_260550, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000163 DASUCY010000000
VERSION     DASUCY010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4664
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260550"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..2117)
                     /locus_tag="VFV25_03180"
     CDS             complement(117..2117)
                     /locus_tag="VFV25_03180"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEY1392346.1"
                     /translation="MRSSFATLAATLAAAMAGAAAQASPAAFTELPGPAFPNYTDAAQ
                     LTQACDAGLKGMQTQLKRLEHHAPDGRWLAAYDDLNASIEDATGPVDLLVNVHPDKAI
                     RDAAQACTLRWQDFSSSLNQNGTLYRSLLKLKPRDAIDRRFAQDLRETFEDAGASLPA
                     PQRERAKQIVDRITDLAQQFDRNIREDGTRVAFTDQELQGVPEAVWKDAKHDDEGRYL
                     LGLDYPTYFPVMQLAENPATRERIWRAKTNEGGDANLKLLGEITQLRQEYAQLFGFKS
                     YDDFALRRRMAGTSQRVESFLADVKQAVAARELQDLAELREAKAKHLGQAPEATKFER
                     WDLLFYTERLRRERYAVDQELFRQYFPPQESLGFVLHVAEKMLGVRYTRVQPRQPLWQ
                     GDVQAYAVSDAASGKPLATLYVDLYPREGKYNHAAVWSFRSGSTRMHRTPEAALVVNF
                     NRKGLTLQELETLLHELGHSLHNNLSATRYTAQWGTSVQRDFVEAPSQMLEDWVYDKK
                     VLKLFAEVCASCKPVPDELVDQAVKAKEFAKGSFYARQRLYASYDIALHGPGAPEPMA
                     LWARMEGATPLGYVPGTKFPAGFSHVAGGYAAGYYGYLWSLVIAMDLRTAFAPDKLDT
                     AVGLRYRKEVLGNGGQRPPESLLKDFLGRETNSQAFYDYLRH"
     gene            complement(2232..3566)
                     /gene="glmM"
                     /locus_tag="VFV25_03185"
     CDS             complement(2232..3566)
                     /gene="glmM"
                     /locus_tag="VFV25_03185"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEY1392347.1"
                     /translation="MSKRQFFGTDGIRGTVGQPPITPDFVLRLGHAVGRVLKRSEARP
                     TVLIGKDPRISGYMLESALEAGFASAGVEVLLTGPLPTPGVAYLTRALRLSLGVVISA
                     SHNPFPDNGIKFFSAKGEKLPDEWELQVEAALQEPPQWVDSTGLGKARRLDDAQGRYV
                     EFCKSSFSGDLSLKGLKIVVDAANGAAYQVAPNVFHELGAEVIAIGCSPDGTNINDGV
                     GATHPEALQREVLARKADYGIALDGDADRLQLVDRNGRLYNGDELLYVMVADRLAQGL
                     PVPGAVGTLMTNMAVELALKKRGVDFVRAKVGDRYVLEELAARGWQLGGEGSGHLLAL
                     DKHTTGDGIISALQVLQATQRTGNTMADLLRGVELFPQKLINVRLQPGQDWKSNARLA
                     AEQQAVDQELAGQGRVLIRPSGTEPLLRVMVEASEASLADRCAQRLAEAVRA"
     gene            complement(3628..4461)
                     /gene="folP"
                     /locus_tag="VFV25_03190"
     CDS             complement(3628..4461)
                     /gene="folP"
                     /locus_tag="VFV25_03190"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEY1392348.1"
                     /translation="MFWQTTRFRIALDRPQVMGIVNVTPDSFSDGGRHDSAAAAIRHC
                     EQLLKEGADILDIGGESSRPGADPLPLDEELRRVQPVLKAACRLGVPVSIDSYKPEVM
                     RAALDLGVDIVNDINALRDPGAEQLVTVHASCGVCLMHMLGRPKSMQASEPVYRDVVV
                     EVREFLGERAERLLAAGVARERIVLDPGIGFGKSVAHNFELLARQPELLALGYPLLAG
                     WSRKSSLGALTGRAVDERLVPSVAAALAAVQRGARIVRVHDVTATVDALKVWQAAGLI
                     G"
BASE COUNT          742 a         1556 c         1559 g          807 t
ORIGIN      
        1 ctgtccagcg gcaagaagct ggaagacctg gcgatctgaa tatctgaact cagcgtgaac
       61 tcaaagaagg gccgcccgtg ggcggccttt tctattgcgg gagccgggtg cgggtttcag
      121 tgtcgcagat agtcgtagaa cgcctgggaa ttggtctccc gcccgaggaa gtccttgagc
      181 aaggactccg gagggcgctg ccccccgttc ccgagcacct ccttgcgata ccgtagaccc
      241 accgccgtat cgagcttgtc gggcgcaaac gcggtgcgca aatccatcgc gatcacgagg
      301 ctccacaaat agccgtagta accggcagca tagccgcccg cgacatgact gaagccggcc
      361 gggaatttag ttcccggcac atagccgagc ggcgtggccc cttccatgcg ggcccacagc
      421 gccatgggct cgggggcgcc ggggccgtgc aaggcgatgt catagctggc atagagccgc
      481 tggcgcgcat agaaactgcc cttggcgaat tccttggcct tgacggcctg atcgaccagc
      541 tcgtctggca ccggcttgca gctcgcacag acttcggcga acagcttcag caccttcttg
      601 tcatagaccc agtcttccag catctgcgag ggcgcctcga cgaaatcgcg ctgcacgctg
      661 gtgccccatt gcgcggtgta gcgcgtggcc gagaggttgt tgtgcagcga gtgccccagc
      721 tcgtgcagca gcgtttccag ctcctgcagg gtcaggccct tgcgattgaa attcaccacc
      781 agcgccgcct cgggcgtgcg gtgcatccgc gtcgagccgc tgcggaagga ccagaccgcg
      841 gcgtggttgt acttcccctc acgcgggtag agatccacat agagcgtcgc cagcggcttg
      901 ccgctcgccg cgtcgctcac cgcataagcc tgcacgtcgc cctgccatag gggctgccgc
      961 ggctgcacgc gcgtgtagcg gacgcccagc atcttctcgg cgacatgcag cacgaagccc
     1021 aggctttctt gcggtgggaa gtactgccgg aagagctcct gatcgacggc atagcgctcg
     1081 cggcgtaagc gctcggtata gaagagcagg tcccagcgct cgaacttggt cgcctcaggc
     1141 gcctggccca agtgcttggc cttggcttcg cgcaattcgg ccaggtcctg cagttcgcgg
     1201 gccgccaccg cctgcttcac gtcggcaagg aagctctcca cgcgctgcga agtgcccgcc
     1261 atgcggcgcc gcagcgcgaa gtcgtcatag ctcttgaagc cgaagagctg ggcgtactcc
     1321 tggcgcagtt gtgtgatctc gcccagcagc ttgaggttcg cgtcgccgcc ctcgttggtc
     1381 ttggcacgcc agatgcgctc gcgcgtggcc gggttctcgg ccagctgcat cacgggaaag
     1441 taggtcgggt agtccaggcc tagcaggtag cggccttcgt cgtcgtgctt ggcgtccttc
     1501 catacggcct cgggcacgcc ctgcagttcc tggtccgtga aagccacccg ggtgccgtct
     1561 tcgcgtatgt tgcgatcgaa ctgctgggcc agatcggtga tgcgatccac aatctgcttg
     1621 gctcgctcac gctgtggcgc gggcaggctc gcaccagcat cctcgaaggt ctcgcgcaga
     1681 tcctgtgcga accgccggtc gatggcgtca cgcggcttga gcttgagcag ggatcgatag
     1741 agcgtgccgt tctgattgag gctggacgag aaatcctgcc agcgcagcgt gcaggcttgg
     1801 gcggcgtcgc ggatcgcctt gtcgggatgc acgttgacca gcaggtccac ggggccggtg
     1861 gcgtcttcga tcgaggcgtt caggtcgtca tatgcggcca gccagcgccc gtccggcgcg
     1921 tggtgctcca ggcgcttgag ctgggtctgc atacctttga ggccagcatc gcaggcctgc
     1981 gtcagctggg ccgcatcggt gtagttcggg aaagcaggcc cgggcagttc ggtaaaggca
     2041 gcaggactcg cttgggctgc ggcaccagcc atggcggcag ccagggtggc cgccagcgtc
     2101 gcaaaactcg atcgcatcta tcctccttgt gggtgaacgg cgggcgactg cggcccgctc
     2161 ggcgaaagcg cgcattgtgc ccgcgtgagg gcacaagaca ggcggtgtat ggcgcgcggc
     2221 gggtgctgcg ttcaggcgcg cacggcctcg gccaggcgct gcgcgcaacg gtcggccagc
     2281 gaggcttcgg aagcctccac catcacgcgc agcaacggct cggtgcccga ggggcggatc
     2341 agcacgcggc cttgaccggc cagctcctga tccacggcct gctgctcggc tgccaggcgc
     2401 gcgttggact tccagtcctg gccgggctgc aggcgcacat tgatgagctt ctgcgggaac
     2461 agctccacgc ctcgcagcaa atcggccatg gtgttgccgg tgcgctgcgt cgcctgcagc
     2521 acctgcagcg cgctgatgat gccgtcgcca gtggtgtgct tgtccagcgc tagcagatgg
     2581 cccgagcctt cgccgcccag ctgccagccg cgcgccgcga gttcctcgag cacatagcgg
     2641 tcgccgacct tggcgcgcac gaagtccacg ccgcgcttct tgagcgccag ctcgaccgcc
     2701 atgttcgtca tcagcgtgcc gacggcgccg gggacgggca ggccctgggc caggcggtcg
     2761 gccaccatga cgtagagcag ctcgtcgccg ttgtagaggc gaccattgcg gtccaccagc
     2821 tgcaggcggt cggcgtcgcc atccagcgcg atgccgtagt cggccttgcg cgccaggacc
     2881 tcgcgctgca gcgcttcggg atgggtggcg cccacgccat cgttgatgtt ggtgccatcg
     2941 gggctgcagc ctatggcaat gacctcggcg ccgagttcgt ggaagacatt gggcgcgacc
     3001 tgataagccg cgccattggc cgcatcgacg acgatcttga gccctttcag cgaaagatcg
     3061 cccgagaagc tgctcttgca gaactcgacg tagcggccct gcgcgtcgtc cagccggcgc
     3121 gccttgccca gccccgtgga atcgacccac tgcggcggct cctgcagggc cgcctcgacc
     3181 tgcagctccc attcgtcggg cagtttttcg cctttggccg agaagaactt gatgccgttg
     3241 tcgggaaagg ggttgtgcga ggcactgatc accacgccca ggctcagcct cagggcgcgc
     3301 gtcagatagg ccacgcccgg ggtgggcagc gggcctgtca gcagcacctc gacgccggcc
     3361 gaggcgaagc ccgcttccag cgccgattcc agcatgtagc ctgagatgcg tggatccttg
     3421 ccgatcagca ccgtcggacg tgcctcactg cgcttgagca cgcggcccac ggcatggccc
     3481 aggcgcaaca cgaaatcagg cgtgatcggg ggctggccga cggtgccgcg tatgccgtca
     3541 gtgccgaaga attggcgttt gctcatggcc ggaattgtgc ctgcgtagcg cgccctgcag
     3601 accccctccg gtaggcggcc agccggctca gccgatcagc cccgcggcct gccagacctt
     3661 cagggcatcg acggtggcgg tcacgtcgtg cacgcgcacg atgcgagcac cgcgctgcac
     3721 agccgccagc gctgccgcga cgctgggcac caggcgctcg tcgaccgcac ggccggtcag
     3781 cgcaccgaga gaagacttgc gcgaccaacc cgccagcagg gggtagccga gcgccaacag
     3841 ctccggctgc cgggccagca actcgaagtt gtgcgccacg ctcttgccga agccgatgcc
     3901 ggggtcgagc acgatacgct cgcgcgccac gccggcagcc agcaggcgct cggcgcgttc
     3961 gccgagaaat tcacgcacct cgaccaccac atcgcgatag accggctcgc tggcctgcat
     4021 ggacttcggc ctaccgagca tgtgcatcag gcagacgccg cagcttgcgt gcactgtcac
     4081 cagctgctct gcgccggggt cgcgcagcgc attgatatcg ttgacgatat cgacgcccag
     4141 atccagcgcg gcccgcatca cttcgggctt gtagctgtcg atggagaccg gcacgcccag
     4201 acggcacgcc gctttcagca ccggctgcac gcggcgcagc tcctcgtcca gcggcaaggg
     4261 atcagcaccg gggcggctgg actcgccgcc gatgtcgagg atgtcggctc cctccttcag
     4321 cagctgctcg cagtggcgta tcgctgcagc cgcactgtca tgccggccgc cgtcggagaa
     4381 ggaatctggc gtcacgttga cgatgcccat cacctgcggg cgatccaagg cgatgcgaaa
     4441 gcgtgtggtc tgccagaaca tgagatttgc cggaaaagga aacggggccg cagccccgtt
     4501 ttattcagcg cttgtgtatc cggccgcttc aggccgtcgc gtgggcgttg tcggtgttgt
     4561 tgaccggcgg cgtggcgccg ccaccgctgc tcttgttgtt cgccggcgac cagtccttgg
     4621 gcggccgcgg cggcttgccg gccatgatgt cgtcgagctg ctcg
//
LOCUS       DASUCY010000164         3422 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1272772, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000164 DASUCY010000000
VERSION     DASUCY010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1272772"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VFV25_03195"
     CDS             complement(<1..329)
                     /locus_tag="VFV25_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY1392349.1"
                     /translation="MLANDVVPQGRTSAAVTMTAGRLAARAGNNGRPSGEGIADALKL
                     LQEKLPLQKRLVRAGDTVYQAGEKFASLYILNAGFFKLLNVSADGREQVVGLKFKGDW
                     LGFDGIAG"
     gene            598..1350
                     /locus_tag="VFV25_03200"
     CDS             598..1350
                     /locus_tag="VFV25_03200"
                     /inference="COORDINATES: protein motif:HMM:NF014749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392350.1"
                     /translation="MVHALPYRHVWSIDSLSRGELLSLLDSALLFKQASQAPDGLPRP
                     LHGKYIAILSDDHASPEAAAFDRAASELGAQVAHVRATDPAARESRDPRDTAGLLGRL
                     YDAIECQGMPPEQVQQIDRHAGVPVFNGIAGARHPLHVLADLMSLQERSGEPFERLRL
                     RFAGDPRGEAGLVLANLADVLGFELVTGQNGSHASQATDLEIGCEPGGELPLQLSLHL
                     GALPQMAGRSLVHEQARNRRCVLQAVLSSTVR"
     gene            complement(1381..2046)
                     /locus_tag="VFV25_03205"
     CDS             complement(1381..2046)
                     /locus_tag="VFV25_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEY1392351.1"
                     /translation="MLVSLLLTALLMGLAGGPHCVAMCGAATAGIGCTSPRLWAFQGG
                     RVLGYALLGAAVAASAGVLQWAATHAALLKPFWAMLQLAILALGLSLVWLGRQPLWLD
                     RWAHQVWQGLRMRSLDWRAAPALAGLLWALLPCGLLYSALMVAALSSHAWQGGLVMAA
                     FAAGSALGLHLGPALWRRWRQAAGPQAGHWGVRLAGLTVACAAAWALGHGLWKEYGAL
                     FCA"
     gene            complement(2046..>3422)
                     /gene="hemN"
                     /locus_tag="VFV25_03210"
     CDS             complement(2046..>3422)
                     /gene="hemN"
                     /locus_tag="VFV25_03210"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEY1392352.1"
                     /translation="ISEQLLARFDVPGPRYTSYPTADRFVEAYTAAQYQEALRQRASG
                     MGGGASTPLSVYVHIPFCESVCYYCACNKIVTKRHEQSDGYLDALEQELALHTRELGT
                     RTPVSQLHLGGGTPTFLSDAQMARLMQALRSSFTLVPGGEHSIEIDPRTVTPERLRFL
                     AELGFNRLSFGVQDFDPDVQRAVHRVQPYDSVAALMRSARELGFESINADLIYGLPRQ
                     TPESFARTLQQISELRPDRIALYAYAHLPQRFKPQRRIVEAELPAPAQKLAMLSAAIA
                     SFIARGYAYIGMDHFALENDALAVAKRQGRLHRNFQGYSTQPECDLIGLGVSAIGRVG
                     ASYSQNAKTLPEYYDALRQGQFPVVRGLALERDDLARRAVIMAIMCQGHLVYESIELA
                     YLLDFKRYFDAELKALQPLADQGLVVLEADGLRVTPMGWYFVRAVAMVFDKHLQAQRN
                     RERYSRII"
BASE COUNT          577 a         1196 c         1140 g          509 t
ORIGIN      
        1 cccgcgatgc cgtcgaagcc cagccagtcg cccttgaact tgaggccgac cacctgctcg
       61 cggccgtcgg ccgacacgtt gagcagcttg aagaagccgg cgttgaggat gtagaggctt
      121 gcgaacttct cccccgcctg atagacggtg tcgccggcac gcaccaaacg cttttgcaga
      181 ggcagctttt cctgcaagag cttgagcgca tcggcgatgc cctcgccaga cggccgcccg
      241 ttgttgccgg cgcgcgcggc cagccggccc gccgtcatcg tcaccgccgc tgaagtccgc
      301 ccttggggta ccacgtcgtt tgccagcatc tcgcgctcct ttgctttgct cgttcgttgc
      361 gacaagctta gggagccccc ggcaatcccg tgacaacggc agaacaccgt gggtaacact
      421 gcgtgaatgc tcggtatcgg ctgcgtaata acccgatggg cgcagcccct gcttgatttg
      481 catcagaagc gggccagttc ggctctctta atctcggcac cggattgttt gcaccagcgc
      541 ctgagagaag ccatggagtc aattgcagcg gcgacaacgc ctagccggag ccgcaccatg
      601 gttcacgccc tgccttaccg ccacgtctgg tcgatcgaca gcctttcgcg cggcgaattg
      661 ctgtccctgc tggacagcgc tctgcttttc aagcaagcga gccaggcgcc cgacggcctg
      721 ccgcggccgc tgcatggcaa gtacatcgcg atcctgagcg acgatcacgc cagccccgag
      781 gcggctgcct tcgatcgtgc cgcctccgag ctcggtgccc aggtcgcaca cgtgcgcgcc
      841 accgatccgg ccgcgcgtga aagccgcgac ccgcgcgaca cggcgggcct gctcggccgc
      901 ctgtatgacg cgatcgaatg ccagggcatg ccgcccgaac aagtgcagca gatcgatcgc
      961 cacgccggcg ttcccgtgtt caacggcatc gccggcgctc gccatccgct gcacgtgctg
     1021 gccgatctga tgagcttgca ggaacgcagc ggcgagccct tcgaacggct gcgcctgcgc
     1081 ttcgccggcg atccccgcgg cgaagccggc ctggtcctcg caaacctggc cgacgtgctg
     1141 ggcttcgaac tcgtgacagg ccagaacgga tctcatgcct cacaggcgac ggacctggag
     1201 atcggctgcg agccgggcgg cgaactcccg ctgcaactct ccttgcatct cggcgccctg
     1261 ccgcagatgg ctgggcgttc gctggtccac gaacaggccc gcaaccgccg ctgcgtactt
     1321 caagcggtgc tgagcagcac ggtgcgatag cccacgcagg ccctgcgggg gcgggtcatt
     1381 tcaagcgcag aacaaagcac cgtattcctt ccacagccca tgccccaggg cccaggcggc
     1441 ggcacaggcc acggtgaggc cggccagcct gacgccccaa tgcccggcct gtggaccggc
     1501 cgcctggcgc cagcgccgcc acagcgccgg gcccaggtgc aggcccagcg ccgagccggc
     1561 ggcgaaggcg gccatgacga ggccgccttg ccaggcgtgt gagctgaggg cggccaccat
     1621 cagggccgaa taaagcagcc cgcagggcag caaggcccag agcaggcccg cgagtgccgg
     1681 cgcggcccgc cagtccaggc tgcgcatgcg caggccctgc cagacctgat gcgcccagcg
     1741 atcgagccat agcggctgac gccccagcca cacgaggctc agccccaggg ccaggatcgc
     1801 gagctgcagc atggcccaga agggcttgag cagcgcagca tgtgtcgcgg cccactgcag
     1861 cacgccggcc gaggccgcga ccgccgcgcc cagcaacgca tagcccagca cgcgcccgcc
     1921 ctggaaggcc cacagccttg gcgacgtgca gcctatgccg gccgtggccg cgccgcacat
     1981 cgccacgcaa tgcgggccgc cggccaggcc catcagcaag gcagtcagaa gcagactgac
     2041 gagcatcaga tgatgcgcga gtagcgctcg cggttgcgtt gcgcctgcag gtgcttgtcg
     2101 aacaccatcg cgacggcgcg cacgaaatac cagcccatcg gagtgacgcg cagtccgtcg
     2161 gcttccagca caaccaggcc ctgatcggcc agcggctgca aggccttcag ctcggcgtcg
     2221 aaataacgct tgaagtcgag cagataggcc agctcgatcg actcgtagac caggtgcccc
     2281 tggcacatga tggccatgat gacggcgcgc cgcgccaggt cgtcgcgctc cagcgccagg
     2341 ccgcgcacga ccggaaactg cccctggcgc aaggcgtcgt agtactcggg cagggtcttg
     2401 gcgttctggc tgtagctggc gccgacccgg ccgatggccg agacgcccag gccgatgagg
     2461 tcgcactccg gctgcgtgct gtagccctgg aagttgcgat gcagccggcc ctggcgcttg
     2521 gccacggcca gcgcatcgtt ctcgagcgcg aagtggtcca tgccgatgta ggcatagccg
     2581 cgcgcgatga agctcgcgat cgcggcgctc agcatggcca gcttctgcgc aggcgccggc
     2641 agctcggcct cgacgatgcg gcgctgcggc ttgaagcgct gcggcagatg cgcataggca
     2701 tatagagcga tgcgatcggg tcgcagctcg ctgatctgct gcagcgtgcg cgcaaacgac
     2761 tcgggagtct gccgcggcag gccgtagatc aggtcggcat tgatcgactc gaagccgagc
     2821 tcgcgcgcac tgcgcatcag cgcggccacg ctgtcgtagg gctgcacgcg gtgcaccgcc
     2881 ctctgcacat cggggtcgaa gtcctgcaca ccgaagctca ggcggttgaa gccgagctcg
     2941 gccaggaagc gcaggcgctc gggcgtcacg gtgcgcggat cgatttcgat ggagtgctcg
     3001 ccgcccggca ccagcgtaaa gctgctgcgc aaggcctgca tcagccgcgc catctgtgca
     3061 tcgctcagga aggtcggcgt gccgccgccc aggtgcagct gcgacaccgg cgtgcgcgtg
     3121 cccagctccc tggtgtgcag cgccaactcc tgctccagcg cgtccaggta gccgtcggac
     3181 tgctcgtgcc gcttggtgac gatcttgttg caggcgcagt agtagcagac cgactcgcag
     3241 aaggggatgt gtacatagac cgacagcggc gtgctcgcgc cgccgcccat gccgctggcg
     3301 cgctgccgca aggcctcctg gtactgggcg gcggtatagg cctcgacgaa gcgatcggcc
     3361 gtcgggtagg aggtgtagcg cgggccgggc acgtcgaagc gggccaggag ctgctcggag
     3421 at
//
LOCUS       DASUCY010000165         2223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_389151, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000165 DASUCY010000000
VERSION     DASUCY010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389151"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="VFV25_03215"
     CDS             complement(<1..895)
                     /locus_tag="VFV25_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEY1392353.1"
                     /translation="MSRHQAIEQTLAFFDDGRFQQTLARRVAFRTESQDAASGPILHA
                     YLDEEIVPQLEALGFTCRVIDNPEAGKSPFLLAERIEPGATFTVLTYGHGDVVRGYDA
                     QWRQGLSPWNIVVEGNRWYGRGTADNKGQHTINLTALEQVLAVRGGQLGYNVKIILEM
                     GEEAGSPGLNEVCALHSELLAADLFIASDGPRVSASRATMFLGSRGVFNFDLHLKLRD
                     GGHHSGNWGGLLRNPGIRLAHAIASLVDAKGKILVPGLRPAELPASVRTALKDIEIGG
                     DVNDPEIDADWGEPGLSAAERV"
     gene            complement(933..1784)
                     /locus_tag="VFV25_03220"
     CDS             complement(933..1784)
                     /locus_tag="VFV25_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY1392354.1"
                     /translation="MNSLLPAGPDIALELCALHCVYPLKRGLFRAPGEIKAVNNVSLR
                     IQKGETLGLVGESGCGKSTLAKMLLGLLAPTSGNVLVNGKEIDTGHRKALARSIQPIF
                     QDPYSSLNPRRTVADIVGVPLRLHQVGTAAEQRKRVREMLDLVGMPERTHSQYPNQLS
                     GGQRQRVAIARALVLRPDILICDEPTSALDVSVQAQILNLLLDLKAEFGLTYLFISHD
                     LGVVEHLVDRVAVMHQGAIVELGTREQIFARPGHPYTRMLLVSALTPEPGLGIPDIDR
                     DLPVALA"
     gene            complement(1793..>2223)
                     /locus_tag="VFV25_03225"
     CDS             complement(1793..>2223)
                     /locus_tag="VFV25_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEY1392355.1"
                     /translation="VTIQAQILRLIRELQKEFGTAVVFITHDLGVVARIADRVAVMYA
                     GEVIETAPVSELFARPTHPYTRGLLNCIPVRGKTLPGSHLQAIAGVVPSLIGNVQGCA
                     FANRCPQVQTACAQTPPYVAVNAQHAARCVAAQLPMAEAA"
BASE COUNT          334 a          698 c          810 g          381 t
ORIGIN      
        1 tcacgcgttc ggctgccgac aggccgggct cgccccaatc ggcgtcgatc tcggggtcgt
       61 tcacgtcgcc gccgatctcg atgtctttca acgcagtgcg cacgctggcg ggcaattcgg
      121 cggggcgcag gccgggcacg agaatcttgc ccttggcatc gaccaggctg gcgatggcat
      181 gcgcgaggcg gatgccgggg ttgcgcagca ggccgcccca gttgcccgag tggtggccgc
      241 cgtcgcgcag cttcaggtgc aggtcgaaat tgaacacgcc gcgcgagccg aggaacatcg
      301 tggcgcgcga tgcggagacg cgcgggccgt cggaggcgat gaacaggtcg gccgcgagca
      361 actcgctgtg cagcgcgcag acctcgttca ggcccggcga gccggcttcc tcgcccatct
      421 ccaggatgat cttcacgttg tagcccagct ggccgccgcg caccgccagc acctgctcca
      481 gcgcggtgag gttgatggtg tgctggccct tgttgtctgc ggtgccgcgg ccgtaccagc
      541 ggttgccttc gaccacgata ttccacgggc tcaggccctg gcgccattgc gcgtcgtagc
      601 cgcgcaccac gtcgccgtgg ccgtaggtca gcacagtgaa ggtggcgccg ggctcgatgc
      661 gctcggccag caggaacggg cttttgccgg cctcggggtt gtcgatgacg cggcaggtga
      721 agcccagtgc ttcgagctgc ggcacgatct cctcgtccag gtaggcatgc aggatcgggc
      781 cgctggcggc gtcctggctt tcggtgcgga aggcgacgcg gcgggccagc gtctgctgga
      841 agcgaccgtc gtcgaaaaag gccagcgtct gttcgatggc ttggtggcgg ctcatgggtg
      901 ttcctctcag tggttttttt ggaatgggaa gctcaggcga gcgcgacggg tagatcgcgg
      961 tcgatgtcgg gaatgccgag gcccggctcg ggcgtgagcg ccgagaccag cagcatgcgg
     1021 gtgtaggggt gtccgggccg cgcgaagatc tgctcgcgcg tgccgagctc cacgatcgcg
     1081 ccctggtgca tcacggccac gcggtcgacc aggtgttcga ccacgccgag gtcgtggctg
     1141 atgaacaggt aggtcaggcc gaactcggcc ttcaggtcga gcagcaggtt gaggatctgc
     1201 gcctgtaccg agacatcgag cgccgaggtc ggctcgtcgc agatcaggat gtcggggcgc
     1261 agcaccaggg cccgggcgat ggccacgcgc tggcgctgac cgcccgagag ctggttgggg
     1321 tactggctgt gggtgcgctc gggcatgccc acgaggtcga gcatctcgcg cacacgttta
     1381 cgctgctcgg cggccgtgcc gacctggtgc aggcgcagag gcacgccgac gatgtcggcc
     1441 accgtgcggc gcgggttcag cgacgaatac gggtcctgga agatcggctg gatcgagcgc
     1501 gccagtgcct tgcggtggcc ggtgtcgatc tccttgccgt tgaccagcac gttgcccgag
     1561 gtgggcgcga gcaggccaag cagcatcttg gccagcgtac ttttgccgca gccggattca
     1621 cccaccaggc cgagggtttc gcccttttgg atgcgcagcg aaacgttgtt caccgccttg
     1681 atttcgccgg gggcacggaa caggccgcgc ttgagtgggt agacgcagtg cagcgcgcac
     1741 agctcgagtg cgatgtccgg gccggcgggc agcagtgaat tcatggctgt gttcatgcgg
     1801 cctccgccat gggaagctgg gcggccacac agcgcgcggc atgctgcgca ttcaccgcca
     1861 catacggcgg cgtttgcgcg caggccgtct gcacctgcgg gcaacggttg gcgaatgcgc
     1921 agccctgcac gttgccgatc aggctgggta ccacgcccgc aatggcctgc agatggctgc
     1981 ccggaagggt cttgccgcgc accggaatgc agttgagcaa gccccgcgtg tacgggtgcg
     2041 tgggccgggc gaacagctca ctcacgggtg cggtttctat cacctcgccg gcgtacatca
     2101 cggccacgcg gtcggcgatg cgcgccacca cgccgagatc gtgggtgatg aagacgaccg
     2161 cggtgccgaa ctctttctgc agctcgcgga tcagccgcag gatctgcgcc tggatggtca
     2221 cgt
//
LOCUS       DASUCY010000166         3627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_19738, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000166 DASUCY010000000
VERSION     DASUCY010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19738"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="VFV25_03230"
     CDS             <1..748
                     /locus_tag="VFV25_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HEY1392356.1"
                     /translation="EVTTAPQNFADTVPVTGEGAETGERGRRRRRRGGRDRGERIEEA
                     GAAEHGEDLAAGEAVSTAGAQPWWAAESEQAAATEAIQTGVEGGIEEEHPGAAEEGER
                     EGSRRRRGRDRNRRERREGEVAEGTAALADAGSGEPLPVLAAVPAGHFLAEPAPAQQP
                     PATALPPELPKAEPFVLPMADLAAVAEAAGLRWVNSDADKVQAAQEAIANTPRLAHVP
                     REPRPVVAIDEGPLVLVETRKDLSQVKLPF"
     gene            complement(919..1974)
                     /gene="nagZ"
                     /locus_tag="VFV25_03235"
     CDS             complement(919..1974)
                     /gene="nagZ"
                     /locus_tag="VFV25_03235"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEY1392357.1"
                     /translation="MNDIQHSPVILDIASTALNDDDRRRLQHPLTGGLIFFTRNWVNR
                     QQITEITAEAKSIRPDLLITIDHEGGRVQRFRTDGFTHLPAMRHLGELWMQDAMRATD
                     AASAAGYVLGAELRACGVDLSWTPVVDLDHGRSEVVGDRAFHRDPRVATLLAKSLMHG
                     LLMAGMANCGKHFPAHGYAVADSHVARAVDDRPLDAILAEDAMPYDWLGTTLTCVMPA
                     HVTFPQVDALPAGFSARWLKDILRGRFGYTGAICCDDLSMEGARVAGSAVEGGIAALN
                     AGCDLVLLCNQSKVDGGRPVDALLDGLQDALERGQWRADADSEARRLALLPQTPPLAW
                     DELMHHPQYQLALERLP"
     gene            complement(1981..2376)
                     /gene="acpS"
                     /locus_tag="VFV25_03240"
     CDS             complement(1981..2376)
                     /gene="acpS"
                     /locus_tag="VFV25_03240"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEY1392358.1"
                     /translation="MIYGVGTDICDIRRIAATLERRGERFAERVLGPHEIEVWRARTA
                     RSAARGLSYLATRFSAKEAFSKAIGLGMRLPMTWQACEVVKAASGKPEIRLHGELAAW
                     FEAKRLRAQVSVSDETEYAAAFVVVETTN"
     gene            complement(2373..3188)
                     /locus_tag="VFV25_03245"
     CDS             complement(2373..3188)
                     /locus_tag="VFV25_03245"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEY1392359.1"
                     /translation="MNPLVAPEAGTAHRGRTALSVNLNKVALLRNTRHLGIPSVRRAA
                     TIALEAGAAGITVHPRPDERHIRAGDVHELAELLRSWPQAEFNIEGNPFHNLMDFVRS
                     VKPHQATFVPDTASQFTSDHGWSLPADMARLAPVIAETKALGVRVSLFMDPQPEAMRL
                     AREAGADRVELYTEAYARAYDSADQTERAEVLDRYVQAAEAALAVGLGVNAGHDLNRD
                     NLTDFLRAVPGVLEVSIGHALIADALELGLDETVRDYQRCIQRAYGPHNDPQR"
     gene            complement(3211..>3627)
                     /locus_tag="VFV25_03250"
     CDS             complement(3211..>3627)
                     /locus_tag="VFV25_03250"
                     /inference="COORDINATES: protein motif:HMM:NF014607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO C-terminal
                     domain-containing protein"
                     /protein_id="HEY1392360.1"
                     /translation="VLFDAYAQTLPLLAAQNDTVVEAALRAFELLLLRETGVLPELSQ
                     VTLTQQPVRAAATYMLRAEAGVAGAAAGEGGIPGQVLLALEAAMQSRELAALQQAASA
                     SLPELKTQLRALLHYHLGSAMLRTRQLMLEVQEQLS"
BASE COUNT          579 a         1226 c         1263 g          559 t
ORIGIN      
        1 ggaagtgacg actgccccgc agaacttcgc cgacaccgtg ccggtcacgg gcgaaggcgc
       61 cgagaccggt gagcgaggcc gccgtcgccg ccgccgaggt ggtcgcgatc gcggtgagcg
      121 catcgaggag gcaggcgcag cagagcacgg cgaagacttg gcggccggtg aagccgtctc
      181 cactgccggc gcgcagccgt ggtgggccgc cgagtccgag caggctgccg cgaccgaggc
      241 catccagacg ggcgtcgagg gcggcatcga agaggaacac ccaggcgcag ccgaggaagg
      301 cgagcgcgaa ggcagccgcc gtcgccgtgg gcgcgatcgt aatcgccgag agcgtcgcga
      361 gggagaagtt gccgaaggca cggctgccct ggccgatgcg ggctctggcg aaccgctacc
      421 tgtgctagcg gctgtacctg ccgggcattt cctcgccgaa cctgcccctg cccagcagcc
      481 gcctgcgaca gcactgccac cggaattgcc caaggccgag cccttcgtgc tgcccatggc
      541 cgatctcgca gccgtcgccg aagcggccgg gctgcgatgg gtcaactcgg atgcggacaa
      601 ggtgcaggca gcccaggagg cgatcgccaa cacgccgagg ctcgcccatg tgccgcgcga
      661 gcctcgccct gtggtggcaa tcgacgaggg cccgctggtg ctggtcgaga cgcgcaagga
      721 cctgtcgcag gtcaagttgc ctttctaagc cccaccaacg cgctgcgcgc gccccctgcg
      781 agggggcgac accggcagac cggcaaagcc ggatctgcgg tgtctgctgg cagcagagat
      841 ttcgcggttc aaccgacacg aaaacgccgc cgggcgcatg cctcggcggc gtttttcatt
      901 ccgaccaagc cgcctgtatc aaggcaggcg ctccagcgcg agttggtact gagggtgatg
      961 catcaattca tcccaggcga gcgggggcgt ctgtggcagc agggccaggc gacgtgcctc
     1021 gctgtcggcg tcggcacgcc actggccacg ctccagggcg tcctgcaggc catcaagcag
     1081 tgcgtccacc ggcctgccgc catcgacctt ggactggttg cacagcagca ccaggtcgca
     1141 gcccgcattg agcgccgcga tgccgccctc gacggcgcta ccggccacac gcgcgccttc
     1201 catgctcagg tcgtcgcaac agatcgcgcc ggtgtagccg aagcgcccgc gcaggatgtc
     1261 cttgagccag cgcgcagaga agccggccgg cagcgcatcg acctgcggga aggtcacatg
     1321 cgccggcatc acgcaggtga gcgtcgtgcc caaccagtcg tagggcatcg cgtcttcagc
     1381 caggatggca tcgagcgggc ggtcgtcgac ggcacgcgcc acatgcgagt cggccactgc
     1441 atagccatgg gccgggaaat gcttgccgca attggccatg ccggccatca gcaggccatg
     1501 catcaggctc ttggcgagca gcgtggccac gcgcgggtcg cggtggaagg cacggtcgcc
     1561 caccacttcg ctgcgaccgt ggtcgaggtc gaccaccggc gtccagctca ggtcgacgcc
     1621 gcaggcgcgc agttcggcac ccagcacata gccggcggca ctggccgcat cggtggcgcg
     1681 catcgcgtct tgcatccaca gttcgcccag gtgacgcatg gccggcagat gggtgaagcc
     1741 atcggtgcgg aagcgctgca cgcgtccgcc ttcatggtcg atggtgatca agagatcggg
     1801 gcgtatggac ttggcctcgg ctgtgatctc cgtgatctgc tgccggttga cccagttgcg
     1861 cgtaaagaag atcagcccgc cggtcagcgg atgctggaga cgccgcctgt cgtcgtcgtt
     1921 gagggccgtg ctggcgatgt cgaggatcac cggcgaatgc tgaatgtcgt tcattcttct
     1981 tcagtttgtc gtttccacca ccacgaaggc ggcagcgtat tcggtttcgt cgctcacgct
     2041 cacctgcgct cgcaaacgct tcgcctcgaa ccaggcggct agctcgccgt gcagccggat
     2101 ctcgggcttg ccgctggcgg ccttgaccac ctcgcaggcc tgccaggtca tcggcaggcg
     2161 catgccgagg ccgatcgcct tggagaaggc ctccttggcc gagaagcgcg tggccagata
     2221 gctcaggcct cgcgcagccg agcgcgccgt gcgcgcacgc cagacctcga tctcgtgtgg
     2281 ccccagcacg cgttccgcga agcgctcgcc gcgccgttcc agcgtcgctg cgatgcggcg
     2341 gatgtcgcag atgtcggtgc cgacgccgta gatcatcgct gcgggtcgtt gtgcgggccg
     2401 taggcccgct gtatgcagcg ctgatagtcg cgcacggttt cgtccaggcc cagctccagc
     2461 gcatcggcga tcagcgcatg gccgatcgag acctcgagca cgccaggcac agcgcgcagg
     2521 aaatcggtga ggttgtcgcg gttcagatcg tggccggcat tgacccccag gccgacggcc
     2581 agcgccgctt cggccgcctg cacgtagcgg tccagcactt cggcccgctc ggtctgatcg
     2641 gcgctgtcgt aagcgcgcgc ataggcctcg gtgtagagct cgacgcggtc ggcgccggcc
     2701 tcgcgcgcca ggcgcattgc ctcgggctga ggatccatga aaaggctcac gcgcacgccc
     2761 aaggccttgg tctcggcaat caccggcgcc aggcgggcca tgtcggccgg caggctccag
     2821 ccgtggtcgg aagtgaactg gctggccgtg tcgggcacga aggtggcctg gtgaggcttc
     2881 acgctgcgca cgaagtccat caggttgtgg aagggattgc cctcgatatt gaactcggcc
     2941 tggggccagg atcgcagcaa ctcggccagc tcgtgcacgt cgccggcgcg gatatggcgc
     3001 tcgtccgggc gcggatgcac cgtgatgccc gccgcgcctg cctccagcgc gatcgtcgcc
     3061 gcccgcctga cgctggggat gcccaagtgg cgcgtgttgc gcagcagcgc caccttgttg
     3121 agattgaccg acagcgcggt gcggccgcga tgcgcggtgc cggcttcggg ggcgaccaga
     3181 ggattcatcg tggatttcgt cgaagcagat tcagctcagc tgctcctgca cctccagcat
     3241 cagctggcgc gtgcgcagca tggcgctgcc gagatgatag tgaagcaagg cgcgcagctg
     3301 ggtcttgagc tcgggcagtg aagcgctggc ggcctgttgc agcgctgcca gctctcggga
     3361 ttgcatggcc gcctcaagcg ccagcagcac ctgcccgggg atgccgccct cgcccgccgc
     3421 ggcgccggca acgccggcct cggcgcgcaa catataggtc gccgccgccc gcaccggctg
     3481 ctgggtcagc gtgacctggc ttagctcggg caggacgccg gtttcgcgca gcagcagcag
     3541 ctcgaaggcg cgcagagcgg cctccaccac ggtgtcgttc tgtgcagcca gcagcggcaa
     3601 tgtctgcgca taggcgtcga acagcac
//
LOCUS       DASUCY010000167         3601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1465417, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000167 DASUCY010000000
VERSION     DASUCY010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1465417"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VFV25_03255"
     CDS             <1..395
                     /locus_tag="VFV25_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY1392361.1"
                     /translation="IQAFSKIGLLPDAGGTWLLPRLVGRAQALGLALLGDKLPAEEAQ
                     RMGLIWKCVDDTALADEAQALAQRLAAMPIKALVSTREALDAAEHLSFAQALDEEARR
                     QRILGSAPDYQEGASAFLAKRAPRFTER"
     gene            409..1932
                     /locus_tag="VFV25_03260"
     CDS             409..1932
                     /locus_tag="VFV25_03260"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEY1392362.1"
                     /translation="MTSYDLNRDTRVAVIGAGSMGAGIAQLAAQAGHAVQLHDAQPGA
                     AARGLERIAADLDVAVRKGKLQSDERAAVLGRIRIAERIEELEGAGLAVEAIIEKAEA
                     KQALFRALEAVLSPRAVFATNTSSISVTAVAQGLKHPQRLIGLHFFNPATRMKLVEII
                     RGVQTDAALVPAMHALSKAWGKTSVDAPNAPGFIVNRVARPYYAEGLRLLGERIAEVP
                     AIDRLLREAGGFAMGPFELMDLIGVEVNLSVTESVFQATAYDPRYAPSVIQQELARSG
                     RYGRKSGRGFYAYAPDAPAPQVAFVEPAASQPRLAQAQQMGLLEPLVQRLQAAGLSVE
                     IDAALPAESFAVNGARVALTDGRTATQRAYEDSTPMLLLDLARDYASATTLGVAACAG
                     EEGRLADLAAALKPAGISLIALADVAGLVVMRSVCCLANEAADVMSWSGARAKDIDVA
                     MVLGTAYPMGPLAWADAIGPSRVRGVLVNLQAHYGEVRYRCAPALSRVHFSKGRFHG"
     gene            1925..2374
                     /gene="paaI"
                     /locus_tag="VFV25_03265"
     CDS             1925..2374
                     /gene="paaI"
                     /locus_tag="VFV25_03265"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HEY1392363.1"
                     /translation="MAEAVQQEAQRTADLVRERMFANDRASQALGMQITEMGPGSATL
                     TMTVRADMLNGFDICHGGHITALADSAFAFACNSHNEMTVASGLTIDFLAPAREGDRL
                     TAQCTEASLSGRTGVYDVSVSNQRGERIAIFRGRSYRIKGKPVVAQA"
     gene            2402..>3601
                     /gene="paaK"
                     /locus_tag="VFV25_03270"
     CDS             2402..>3601
                     /gene="paaK"
                     /locus_tag="VFV25_03270"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HEY1392364.1"
                     /translation="MNVSPPQPAELEPIERASRDEIAALQLQRLRWTLRHAYEHVPHY
                     RAAFDAKGVHPSDLKQLSDLAKFPFTTKKDLRDNYPFGLFAVPREQVVRIHASSGTTG
                     KPTVVGYTRKDIDTWAGLVARSIRAAGARPGDLVHVSYGYGLFTGGLGAHYGAERLGC
                     TVIPMSGGQTEKQVQLIRDFKPRVIMVTPSYMQVIIEEFARQGLDARESSLEIGIFGA
                     EPWTEAMRHEIEAKADIKAVDIYGLSEVMGPGVASECVETQDGPVIWEDHFYPEIIDP
                     ETGAVLPDGSEGELVFTSLSKEALPIIRYRTRDLTRLLPPTARAFRRMGKIVGRSDDM
                     LIIRGVNVFPTQIEEIVLQHEKLSGQYQIHVMREGLLDAVEVHCELQPASAGLADSEV
                     REIAGWVA"
BASE COUNT          575 a         1231 c         1259 g          536 t
ORIGIN      
        1 tcatccaggc cttctccaag atcggcctgc tgcccgatgc cggcggcacc tggctgctgc
       61 cgcgcctggt cggccgcgcc caggcgctgg gcctggcact gctgggggac aagctgcccg
      121 ccgaggaagc gcagcgcatg ggcctgatct ggaaatgtgt ggacgacacg gcgctggctg
      181 acgaagccca ggccctcgcg cagcgcttgg ccgcgatgcc catcaaggcg ctggtgtcca
      241 cgcgcgaggc cctggatgcc gccgagcatc tgagcttcgc ccaggcgctg gacgaagagg
      301 cgcggcgcca gcgcatcctc ggctctgcgc ccgactacca ggagggcgcg agcgccttcc
      361 tggccaagcg cgccccgcgg ttcaccgagc gataaggcaa cgcatgtcat gaccagctac
      421 gacttgaatc gagacacccg ggtcgccgtg atcggcgccg gcagcatggg cgccggcatc
      481 gcccagctcg cagcgcaggc ggggcacgcg gtgcagctgc acgatgccca gcccggcgcc
      541 gcggcgcgcg gcctggagcg catcgctgcc gacctggatg tggcggtcag gaaaggcaag
      601 ctgcagtccg acgagcgtgc ggccgtcctg ggccgcattc gcatcgccga gcgcattgag
      661 gagctcgaag gcgcgggcct cgcagtcgag gccatcatcg agaaggccga ggccaagcag
      721 gcgctgttcc gcgcactcga agcggtgttg agcccgcggg cggtgttcgc caccaacacc
      781 tcatcgatct cggttacggc agtggctcag ggcctcaagc atccgcagcg gctgatcggc
      841 ctgcatttct tcaacccggc cacgcgcatg aagctggtcg agatcatccg cggcgtccag
      901 accgatgcgg cgctggtacc ggcaatgcac gcgctgtcca aggcctgggg caagacctcg
      961 gtggacgcgc ccaacgcgcc gggcttcatc gtcaaccgcg tggcgcggcc ttactacgcg
     1021 gagggcctgc gcctgctcgg ggagcgcatc gccgaggtgc ctgccatcga ccgcctgctg
     1081 cgcgaggccg gcggcttcgc gatggggccc ttcgagctga tggatctgat cggcgtcgag
     1141 gtcaatctct cggtcaccga atcggtgttc caggccacgg cctacgatcc gcgctacgcg
     1201 cccagcgtca tccagcagga actggcgcgc agtggccgct atgggcgcaa gagcgggcgc
     1261 ggcttctatg cctatgcgcc ggatgcgccg gcaccccagg tggctttcgt cgagccggcc
     1321 gcgagccagc cgcgcctcgc ccaggcgcag cagatgggct tgctcgagcc gctggtgcag
     1381 cgcctgcagg ccgccggctt aagcgtggag atcgacgcgg cgctgcccgc ggagagcttc
     1441 gcggtgaatg gcgcgcgggt cgcgttgacc gacgggcgca ccgcgaccca gcgtgcctac
     1501 gaagacagca ctccgatgct gctgctcgac ctggcgcgcg actacgccag cgccacaacg
     1561 cttggcgtgg ccgcgtgcgc aggcgaggag ggcaggctgg cggacctggc cgccgccttg
     1621 aagcccgccg gcatctcgct gatcgccctg gccgacgtgg ccgggctggt cgtgatgcgc
     1681 agcgtgtgct gcctcgcgaa cgaggcggcc gacgtgatga gctggtcggg cgcgcgtgcc
     1741 aaggacatcg atgtcgcgat ggtgctgggc accgcctatc cgatggggcc gctggcatgg
     1801 gccgacgcga tcggcccgtc gcgcgtgcgc ggcgtgctcg tcaacctgca ggcccattac
     1861 ggcgaggtgc gctatcgctg cgcgcccgcg ctgagccgtg tgcatttctc gaaagggcgt
     1921 ttccatggct gaggctgtgc agcaagaggc ccagcgcacc gcagatctgg tgcgcgagcg
     1981 catgttcgcc aacgatcgcg cgtcccaggc cctgggcatg cagatcaccg agatgggccc
     2041 gggaagcgcg acgctgacga tgacggtgcg cgccgacatg ctcaacggct tcgacatctg
     2101 ccatggcggc cacatcacgg cgctggccga ctcggccttc gccttcgcct gcaactcgca
     2161 caacgagatg acggtggcct ccggcctcac gatcgacttc ctggcgccgg cgcgcgaagg
     2221 cgaccggctc acggcccaat gcaccgaagc ctcgctcagc gggcgcaccg gcgtctacga
     2281 cgtgtccgtg agcaaccagc gcggcgagcg cattgcaatc tttcgcggcc gctcgtatcg
     2341 aatcaagggc aagccggtgg tggcgcaagc ctgaacccga catgttgcgg agacccattc
     2401 catgaacgtc agtccacccc agccggccga actggagccg atagagcggg ccagccgcga
     2461 cgagatcgcc gcgctgcagc tgcagcgcct gcgctggacg ctgcggcatg cctacgagca
     2521 tgtgccgcac taccgcgccg ccttcgatgc caagggtgtg cacccgagcg atctcaagca
     2581 gctgagcgac ctcgcgaagt ttcccttcac gaccaagaag gacctgcgcg acaactaccc
     2641 cttcggcctc ttcgccgtgc cgcgcgagca ggtggtgcgt atccatgcct cgtcgggcac
     2701 cacgggcaag cccacggtgg tcggctatac gcgcaaggac atcgacacct gggccggcct
     2761 ggtggcgcgc tcgatacgcg cggccggcgc gcgccccggc gacctggtgc acgtctccta
     2821 cggctatggc ctcttcaccg gcggcttggg cgcgcactat ggagccgaga ggctgggctg
     2881 cacggtgatc ccgatgtcgg gcgggcagac cgagaagcag gtgcagctga tccgcgactt
     2941 caagccgcgc gtcatcatgg tcacgcccag ctacatgcag gtgatcatcg aggagttcgc
     3001 gcgccagggc ctggatgcgc gcgagtcctc gctggagatc ggcatcttcg gagccgagcc
     3061 ctggaccgag gcgatgcggc acgagatcga ggccaaggcc gacatcaagg cggtggacat
     3121 ctacggcctg tcggaagtga tgggcccggg cgtggccagc gagtgcgtgg agacgcagga
     3181 tggcccggtg atctgggagg accatttcta tcccgagatc atcgaccccg agaccggcgc
     3241 ggtgctgccc gacggcagcg agggcgagct cgtcttcacc tcgctcagca aggaggccct
     3301 gcccatcatc cgctatcgca cgcgcgacct gacgcgtctg ctgccgccca ccgcgcgggc
     3361 cttccggcgc atgggcaaga tcgtcggccg cagcgacgac atgctgatca tccgcggcgt
     3421 caatgtcttc ccgacgcaga tcgaggagat cgtgctgcag cacgagaagc tctcgggcca
     3481 gtatcagatt catgtgatgc gcgaaggcct gcttgacgcc gtcgaggtgc attgcgagct
     3541 gcagccggcc agcgccggcc tggctgacag cgaggtgcgg gagatcgccg gatgggtggc
     3601 g
//
LOCUS       DASUCY010000168         3367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_308962, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000168 DASUCY010000000
VERSION     DASUCY010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308962"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..731)
                     /locus_tag="VFV25_03275"
     CDS             complement(69..731)
                     /locus_tag="VFV25_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhaM family polyhydroxyalkanoate granule
                     multifunctional regulatory protein"
                     /protein_id="HEY1392365.1"
                     /translation="MSDRNASTPLDQALAFGKLVPGFDFLQGLVKNAGSALPDIGKWV
                     APTLNPDDLEKRIDELRTVQFWLEQNARMLAATIQALEVQRMTLSTLRSMNVPVDLLR
                     DSLAARAAPAAAAGKLPAAKSGAATKPSTGKPTAEKAAAPVDPMQWWGALTKQFTELA
                     TSAMKEAAADSAKNLASGLAKQSFNAAEQTLKAGMAPAKKAAAVARKAAARKKVEPAS
                     RS"
     gene            complement(820..1788)
                     /locus_tag="VFV25_03280"
     CDS             complement(820..1788)
                     /locus_tag="VFV25_03280"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HEY1392366.1"
                     /translation="MPDRPLRRRLACLLFTLCCLAGARAEEAATACPPAAKIPTPEQA
                     KAGQEQARDHGFLWRISKGGHSSWLYGTLHVGKAAWIYPGPQLKAALREADTVALELD
                     MLDPAVLRRLTQAVQAASAPELPAPLTVRLARQIKFACLDAQALKTLSPAMQAATLVA
                     LAGRRDGLDPSYGIDPMLAAAAHRAGKKVVSLETPESQAQALGGETPEEELDFVEQTL
                     QELESGKARPTLVRIARVWASGDLAELERYEQWCDCMNTAAERAQMHRLLDERNAGMA
                     NGIAALHSGGASVLAGVGSLHMIGSQGLPALLAARGYTVERIRFAP"
     gene            complement(1896..2915)
                     /locus_tag="VFV25_03285"
     CDS             complement(1896..2915)
                     /locus_tag="VFV25_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY1392367.1"
                     /translation="MKRTLIVAALASAAAAPALAQSSVTVYGRVNISLERQKNDADQT
                     DNKMVNDSSRFGLRGKEDLGGGMYADFNLESGFNATNGAVTQGGVFWRRQSTVGLGSN
                     DFGHLRLGNIWLTEAYHATADWVSLHNHDTGTSSDAFYGLGGNVAGGLMQHTVAYTSP
                     TFSGLKLDVQYGLNDGEGARRYAGAAQYDVGPLHAALGYEQFNGLKAVTAAGLYTIDA
                     FTFGGYVERDSGTPDIAALSPAGLAGGASRTSVRLVGMYALGASEFHLNVGKAGKVDS
                     VSGSGAKQYTLAYNYNLSKRTKVYTFYTKVDEDGALGTAGRALATSLYGGNFSSFAVG
                     LRHNF"
     gene            complement(3114..>3367)
                     /locus_tag="VFV25_03290"
     CDS             complement(3114..>3367)
                     /locus_tag="VFV25_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008351845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase"
                     /protein_id="HEY1392368.1"
                     /translation="IAHIAKTRNVRAGSIVGSGTVSNKDWSRGYSCIAEKRAIETIEH
                     GQPLTEFMRYADSIRIEMKDREGMSVFGAIDQKVAAPAP"
BASE COUNT          507 a         1096 c         1147 g          617 t
ORIGIN      
        1 ggcgctggca aaggctcgca tcttgatgga tgcccgttgg atgcccgttt ctgggcaggc
       61 aggcgtcgct agctcctgct ggcgggctcc accttcttgc gcgcggcagc cttgcgcgcc
      121 accgcggcgg ccttcttcgc cggggccatg ccggccttca gggtctgttc ggcggcattg
      181 aagctttgct tggccaggcc gctcgccagg ttcttggccg agtcggccgc ggcctccttc
      241 attgcactgg tggcgagctc ggtgaattgc ttggtcagcg ccccccacca ttgcatgggg
      301 tcgaccggag cagccgcctt ctcggccgtc ggcttgccag tcgaaggctt ggtcgccgca
      361 ccgctctttg ccgcgggcag cttcccggcg gccgcggcgg gcgccgcgcg tgcggccaga
      421 gagtcgcgca gcaggtcgac cggaacgttc atgctcctga gcgtggacag cgtcatgcgc
      481 tgcacttcca gcgcctggat cgtggcggcc agcatacggg cgttctgctc cagccagaac
      541 tgcacggtgc gcagctcgtc gatgcgtttc tcaaggtcgt cgggattcag cgtcggcgcg
      601 acccatttgc cgatgtccgg cagcgccgag ccggcgttct tgaccaggcc ctgcaggaag
      661 tcgaagccgg gcaccagctt gccgaaggcg agcgcctggt caagtggggt ggaggcattg
      721 cggtcgctca tgttcgtgtc tcctcgatgg cgttcttgtc ggcgcgagcc gaaaccattg
      781 tgcgcctcgg gcccggcatg cgaaagacgg tggagcaaat caaggcgcga agcggatgcg
      841 ttccaccgtg tagccgcggg ccgccagcag cgccggcagg ccttggctgc cgatcatgtg
      901 caggctgccg acgcccgcca gcaccgaggc gccgccgctg tgcagcgcgg cgattccatt
      961 ggccattccg gcattgcgtt catcgagcag gcggtgcatc tgcgcgcgct cggccgcggt
     1021 gttcatgcag tcgcaccatt gctcgtagcg ttccagctcg gccaggtcgc cgctggccca
     1081 gaccctggcg atgcgcacga gggtcggcct cgccttgccg ctctccagct cctgcagcgt
     1141 ctgctccacg aagtccagct cctcctcagg cgtttcgccg cccagcgcct gggcctgcga
     1201 ctccggcgtc tccagagaca cgaccttctt gcctgcccgg tgcgccgcgg cggccagcat
     1261 gggatcgatg ccgtagctcg ggtccagccc atcgcggcga ccggccagtg ccacgagcgt
     1321 cgcggcctgc atggccggcg acagcgtctt cagggcctgg gcgtccaggc aggcgaactt
     1381 gatctgccgt gccaggcgca cagtcagcgg cgccggcaac tcgggcgcgc ttgcagcctg
     1441 gaccgcctgc gtgagccggc gcagcacggc cgggtccagc atgtccagct ccagggctac
     1501 tgtatcggcc tcacgcagcg ctgccttcag ctgcgggcca gggtagatcc aggccgcttt
     1561 gcccacgtgc agggtgccat agagccagga gctgtggccg cccttgctga tgcgccacag
     1621 gaagccgtga tcgcgcgcct gctcctggcc ggccttggcc tgctcaggcg tcgggatttt
     1681 ggctgcaggt gggcaggctg ttgcagcctc ctcggctcgc gcgccggcca ggcagcacag
     1741 ggtgaacaac aggcaggcga gccggcgacg gagcggacga tcaggcatgg catcggtagg
     1801 tggcatcttc cattcgcgca tcctcgcatg cggcggcacg aaaagaaaaa cggccccgcg
     1861 gggccgtttt cgatcgagag cttatgcagc ccggatcaga agttgtggcg caggcccaca
     1921 gcgaaggagc tgaagttgcc gccgtacagc gaagtggcca gggcgcgacc agcagtgccc
     1981 agcgcgccgt cttcgtcgac cttcgtgtag aaggtataga ccttggtgcg cttggacagg
     2041 ttgtagttgt aggccagggt gtactgcttg gcgccgctgc cactgacgga gtccaccttg
     2101 ccggccttgc cgacgttcag gtggaactcg ctggcaccca gtgcatacat gccgaccagg
     2161 cgcacgctgg tgcgcgaggc gccgccggcc aagccggcag ggctcagcgc agcaatgtcc
     2221 ggcgtgcccg aatcgcgctc gacatagccg ccgaacgtga aggcgtcgat cgtgtacagg
     2281 ccggccgccg tgaccgcctt gaggccgttg aactgctcgt agcccagggc ggcgtgcaga
     2341 gggccgacgt cgtactgagc ggctccagcg tagcggcgcg caccttcgcc gtcattgaga
     2401 ccgtactgga cgtccagctt caggccgctg aaggtgggcg aggtgtaggc gaccgtgtgc
     2461 tgcatcaggc cgccggccac gttgccgccc agaccgtaga aggcgtcgga cgatgtgccg
     2521 gtatcgtggt tgtgcagact cacccagtca gccgtcgcgt gataagcctc ggtgagccag
     2581 atgttgccca ggcgcagatg accgaagtcg ttgctgccca ggccgaccgt ggactggcgt
     2641 ctccaaaaga cacccccttg ggtcaccgcg ccgttggtcg cgttgaaacc gctttccagg
     2701 ttgaagtcgg catacattcc gccgcccagg tcttccttac cgcggagacc gaagcgcgaa
     2761 gagtcgttca ccatcttgtt gtccgtctgg tcggcgtcgt tcttctggcg ctcaaggctg
     2821 atgttgacgc ggccatagac ggtgacggaa ctctgagcga gggcgggagc ggcggcggcg
     2881 ctagccaggg ccgccacgat gagcgtgcgt ttcattgcgt gttctccgaa gagttgtctg
     2941 atttgcgagc gcgcttcggc gtgaagcagg tcacgttttt tgggcgcaag ccagactata
     3001 tgaaacagcc acgaacccgc ggccggaaat cgcgagccga ggctgctttc gtcagcaaat
     3061 tgatgcgtgg gcgcaacggt tttcaggcgc gcaagcggcg agcaccagct ggctcaaggt
     3121 gccggcgcag ccactttctg gtcgatggcg ccgaagacgc tcataccttc gcgatccttc
     3181 atctcgatgc gtatcgagtc ggcgtacctc atgaactcgg tgagcggctg gccgtgctct
     3241 atggtctcga tcgcgcgctt ttcggcgatg cagctgtagc cgcggctcca gtccttgttg
     3301 ctcacggtgc cgctgcccac gatggagccg gcgcgcacat tgcgcgtctt ggcgatatga
     3361 gcgatca
//
LOCUS       DASUCY010000169         2492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_630259, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000169 DASUCY010000000
VERSION     DASUCY010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630259"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VFV25_03295"
     CDS             <1..276
                     /locus_tag="VFV25_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrpF domain-containing protein"
                     /protein_id="HEY1392369.1"
                     /translation="RALSMGKLHHAMMGTAAVAIGTAAAIPGTLVNLAAGGGTRQAVR
                     FGHPSGTLRVGAEASQVDGEWTVTKAVMSRSARVLMEGWVRVPGGLF"
     gene            538..1107
                     /locus_tag="VFV25_03300"
     CDS             538..1107
                     /locus_tag="VFV25_03300"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEY1392370.1"
                     /translation="MLLMIDNYDSFTYNLVQYFGELGEEVRTYRNDEISIEQIEALRP
                     QRLVLSPGPCSPAEAGICVEAVRHFAGKLPILGVCLGHQSIGAALGGRIVRAQRQMHG
                     KTSVITTDERGVYTGLPRDFTVIRYHSLAIAEASLPDALEITARSEDGEIMGVRHKSL
                     PIEGVQFHPESILTEHGHAMLKNFLEQTH"
     gene            1117..2151
                     /gene="trpD"
                     /locus_tag="VFV25_03305"
     CDS             1117..2151
                     /gene="trpD"
                     /locus_tag="VFV25_03305"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEY1392371.1"
                     /translation="MTISNTDALQRTIEHREIFHDEMLALMRRIMGGEMSPVMIAALA
                     VGLRVKKETVGEIAAAAQVMREFATPVHVADKSRLVDLCGTGGDAAHTFNISTAAMFV
                     AAAAGARVAKHGGRSVSSSTGSADVLEALGAHIELTPEQVATCLAETGIGFMFAPKHH
                     AAMKHAAPVRKELGVRTIFNILGPLTNPAAAPNQLMGVFHPDLVGIQVRVLQRLGSEH
                     VLIVYGMNGMDEISLSGETMIGELKNGEVLEYVVHPSDFGLPVYDSRVLKVANREESV
                     QCIERAFANEDGPVRDIVLLNAAGALYAANVVASIADGVKLAREAVASGKALAKLSQF
                     VAVTQKLKSA"
     gene            2155..>2492
                     /gene="trpC"
                     /locus_tag="VFV25_03310"
     CDS             2155..>2492
                     /gene="trpC"
                     /locus_tag="VFV25_03310"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734525.1"
                     /note="involved in tryptophan biosynthesis; amino acid
                     biosynthesis; converts
                     1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate to
                     C(1)-(3-indolyl)-glycerol 3-phosphate and carbon dioxide
                     and water; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase TrpC"
                     /protein_id="HEY1392372.1"
                     /translation="MGDILNKIVAVKHEEIAAALARRSLASLRAEAESAERRADLRDF
                     EGALRRKIAAGKAAVIAEVKKASPSKGVLRESFVPAEIAASYAKHGAASLSVLTDQRF
                     FQGAAEYLQQA"
BASE COUNT          425 a          822 c          853 g          392 t
ORIGIN      
        1 cgggcgctct cgatgggcaa gctgcaccac gcgatgatgg gcacggccgc cgtggccatc
       61 ggcaccgcgg cggcgatccc gggcacgctg gtgaacctcg cggccggcgg cggcacgcgc
      121 caggcggtgc gcttcggcca cccttcaggc accttgcgcg tgggcgcgga agccagccag
      181 gttgacggcg aatggaccgt gaccaaggcc gtcatgagcc gcagcgctcg cgtcctgatg
      241 gaaggctggg tgcgcgtgcc gggcggcttg ttctgattgc cccgacccgg cccccatggg
      301 cccgccggct ggcaccctga tggcggctgc gcaataatgc ggcccatcat gtcctcgacg
      361 cgttcattca cagcgacgct cttcggttgg ccttggcgca agccaaggaa ctgacccacc
      421 cctctttggc gccccgcggc gccagctaag tcccgcagcg ccgtcccggc gcggttcgcg
      481 gagaaacatc gaacggagcg cgagacgcgc cccagtgaat cgagaggatt gagcgccatg
      541 ctgttgatga tcgacaacta cgactccttc acctacaacc tcgtgcagta tttcggcgag
      601 ctgggcgagg aggtgcgcac ctatcgcaac gacgagatca gcatcgaaca gatcgaggcc
      661 ctcaggccgc agcgcctggt gctgtcgccc ggcccctgtt cgccggccga ggcgggcatc
      721 tgcgtcgagg cggtgcggca tttcgccggc aagctgccga tactgggcgt gtgcctgggg
      781 caccagagca taggtgccgc gctgggcggc cgcatcgtgc gcgcgcagcg ccagatgcac
      841 ggcaagacca gcgtcatcac gaccgacgaa cgcggcgtct acaccgggct gccgcgcgat
      901 ttcacggtga tccgctatca ctcgctggcc atagcagagg ccagcctgcc cgacgcgctg
      961 gagatcacgg cgcgttcgga agacggcgag atcatgggtg tgcggcacaa gagccttccg
     1021 atcgagggcg tgcagttcca tcccgagtcc atcctgaccg agcatggtca tgcgatgttg
     1081 aagaactttc tcgaacagac tcattgagga acgcacatga ccatcagcaa caccgatgcg
     1141 ctgcagcgca cgatcgagca ccgcgagatc ttccacgacg agatgctggc gctgatgcgt
     1201 cgcatcatgg gcggcgagat gtcgccggtg atgattgccg ccctggccgt gggcctgcgc
     1261 gtcaagaagg aaaccgtggg cgagatcgcc gccgccgccc aggtgatgcg cgaattcgcc
     1321 acgccggtgc atgtggccga caagagccgg ctcgtggacc tgtgcggtac cggcggcgat
     1381 gcggctcaca ccttcaacat ctccaccgcc gcgatgttcg tcgccgcggc ggccggcgcg
     1441 cgcgtggcca agcatggcgg gcgcagcgtg tcgtccagca ccggcagcgc cgacgtgctg
     1501 gaggcgctgg gcgcgcatat cgaactcacg ccggagcagg tggcgacctg cctggccgag
     1561 accggcatcg gcttcatgtt cgcgcccaag caccacgcgg ccatgaagca tgccgcgccg
     1621 gtgcgcaagg agctgggcgt gcgcaccatc ttcaacatcc tgggaccgct caccaacccg
     1681 gcggcggcgc ccaaccagct gatgggcgtg ttccaccccg atctggtcgg catccaggtg
     1741 cgcgtgctgc agcgcctggg ctctgagcat gtgctgatcg tctacggcat gaacggcatg
     1801 gacgagatct cgctgtccgg cgagaccatg atcggcgagc tgaagaacgg cgaagtgctc
     1861 gaatacgtcg tccaccccag cgacttcggc ctgccggtct acgacagccg cgtgctcaag
     1921 gtggcgaatc gcgaggaatc ggtgcagtgc atcgagcgcg ccttcgccaa cgaggatggg
     1981 ccagtgcgcg acatcgtgct gctcaacgcg gccggcgcgc tctatgcggc caacgtcgtg
     2041 gcctcaattg cggacggcgt caagctggcg cgcgaggcgg tggcttcggg caaggcgctg
     2101 gccaagctga gccagttcgt cgctgtgacc cagaaattga aatccgcctg agccatgggc
     2161 gatatcctga acaagattgt tgcggtcaag catgaggaga ttgccgcggc gcttgcgcgg
     2221 cgctcgctgg cttcgctacg tgctgaggcc gagtcggccg agcggcgcgc cgatctgcgc
     2281 gatttcgagg gcgccttgcg ccgcaagatc gcggcaggca aggcggcggt catcgccgag
     2341 gtcaagaagg ccagccccag caagggcgtg ctgcgcgagt ctttcgtgcc ggccgagatt
     2401 gccgcgagct atgccaagca cggcgcggcc tccctgagcg tgctcaccga ccagcgcttc
     2461 ttccagggcg cggctgagta tttgcagcag gc
//
LOCUS       DASUCY010000170         2168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1288740, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000170 DASUCY010000000
VERSION     DASUCY010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1288740"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1365
                     /locus_tag="VFV25_03315"
     CDS             <1..1365
                     /locus_tag="VFV25_03315"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY1392373.1"
                     /translation="GRVAGHDLLSPELDDRFARDQLNVQLDAAQYEAGQAWVWVDGDY
                     SRLSSTPLSIRLRSLDEVNRSSLMHARTVAASASALGVLSLMALLLWLSLRTEEMLYY
                     GLYVGSQALYLLYIMGEGFAFAPFSLLAPLGSLTWILPAVSGLYFSVCFFSSLMSLHL
                     RMPRVDTAVRWLAVINGVLGIGSVFAPGQAQFLLLSVANLVLILPCLAMLFLAARFAL
                     GGSRHAQLFVTGYALLIVFTVLRTWQLGTAQVPSGWIYYGLPAAMVISALLLVAALAE
                     QALEERRALAAARAEAERDPLTGVRNRRSIEAFVLAALRRAERSQKPMSLLYMDLDHF
                     KRINDTHGHGRGDDCLQAFVRLAQHELREGDALGRVGGEEFLAVLRDTDALAARSIAE
                     RIRATVADAPAAHGEEPIHLTVSIGVACVPPLTASVDHVIDAADRALYRAKAAGRNRV
                     EFFA"
     gene            1427..>2168
                     /locus_tag="VFV25_03320"
     CDS             1427..>2168
                     /locus_tag="VFV25_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEY1392374.1"
                     /translation="MSSAIALLLHPQSKDLGGGLMVRRALPAAARQAVGPFLFFDHFG
                     PITAGPADDHDVRPHPHIGLATVTYLFEGTQIHRDSTGVVQRIEPGAVNWMTAGRGVV
                     HSERTPPELRQAMRVSHGLQLWAGLPEADEELEPSFSHTAASEIPAATLGEVQARLLI
                     GEAFGLRSPVPTRSPTLYIDFALPPGAAVRLPGAAQERAIYSVDAGVLLDDAPLAACA
                     MAVLHPDSEPMLQAPQGARVVMIGGAPLG"
BASE COUNT          278 a          780 c          741 g          369 t
ORIGIN      
        1 ggccgcgtgg caggccacga cctgctgtct ccggagctgg acgaccgctt cgcgcgcgac
       61 cagctcaacg tccagctcga tgccgcgcag tacgaggccg gccaggcctg ggtctgggtc
      121 gacggcgact actcgcgcct gtccagcacg ccgctgagca tacgtctgcg cagcctggac
      181 gaggttaacc gcagcagcct catgcacgcc cgtaccgtgg ccgccagtgc atcggccctc
      241 ggcgtgctgt cgttgatggc gctgctgctg tggctgtcgc tgcgcaccga agagatgctg
      301 tactacggcc tttacgtcgg ctcgcaggcc ctgtacctcc tctacatcat gggcgagggc
      361 ttcgcattcg cgccgttctc gctgcttgcg ccgctgggct cgctgacctg gatcctgccg
      421 gccgtcagcg gcctgtactt ctcggtctgc ttcttcagct cgctgatgtc cttgcatctg
      481 cgcatgccgc gtgtcgacac ggccgtgagg tggctggcgg tgatcaacgg cgtgctgggg
      541 atcggcagcg tcttcgcccc gggccaggcg cagttcctgt tgctgagcgt ggccaacctg
      601 gtgctgatcc tgccttgcct cgcgatgctc ttcctggccg ccaggttcgc gctgggcggt
      661 agccgtcatg cccagctctt cgtcaccggc tatgcgctgc tgatcgtctt caccgtgctg
      721 cgcacctggc agctcggcac ggcgcaggtg cccagcggct ggatctacta cggcctgccg
      781 gccgcgatgg tcatttcggc gctgctgctg gtggccgcgc tggccgagca ggccctcgag
      841 gagcgccgcg cgctggcagc agcgcgcgcc gaggccgagc gcgacccgct gaccggcgtg
      901 cgcaaccggc gctccatcga ggccttcgtg ctggcggctt tgcgcagggc cgagcgcagc
      961 cagaagccga tgtcgctgct ctacatggac ctcgaccact tcaagcgtat caacgacacc
     1021 catggccacg gccgcggcga cgactgcctg caggccttcg ttaggcttgc ccagcacgag
     1081 ctgcgcgagg gtgatgcgct gggacgggtc ggcggcgagg aattcctggc cgtgctgcgc
     1141 gataccgatg ccctcgcggc ccggagcatc gccgagcgca tccgcgctac ggtggcggac
     1201 gctccggcag cccatggcga ggagcccatc cacctcacag tgagcatagg cgtggcctgc
     1261 gtgccgccct tgacggcctc ggtggaccat gtcatcgacg cagccgaccg agcgctctac
     1321 cgcgccaagg cggccgggcg caaccgcgtc gagttctttg cctaggaagc tggcacagta
     1381 aggcgctgtg ccgtcccctg ccggcggcgt gttgaggagc gaacccatga gttcagcgat
     1441 cgcgttgctg ctgcacccgc agtccaagga cttgggcggc ggcctcatgg tgcgccgtgc
     1501 gctgcctgcc gcggcccgcc aggcggtcgg ccccttcctg ttcttcgacc acttcggtcc
     1561 catcacggcc gggcccgctg acgatcacga cgtgcgtccg catccgcata tcggcctcgc
     1621 gaccgtcacc tatctcttcg aagggacgca gatccatcgc gattccaccg gcgtcgtgca
     1681 gcgcatagag ccgggcgcgg tcaactggat gaccgcgggc cgcggcgtcg ttcattccga
     1741 gcgcacgccg cccgagctgc gccaggccat gcgcgtgagc cacggcctgc agctgtgggc
     1801 cgggctgccc gaggccgacg aggagctgga gcccagcttc tcgcacacgg ccgcgagcga
     1861 gatccccgcc gcgacgctgg gcgaggtgca ggcccgcctg ctgatcggcg aggcctttgg
     1921 tctgcgctcg ccggtgccga cgcgctcgcc cacgctctac atcgatttcg ccctgccgcc
     1981 cggcgcggcc gtgaggctgc cgggtgccgc ccaggagcgg gccatctaca gcgtcgatgc
     2041 cggcgtgctg ctcgacgacg cgccgctagc cgcttgcgcg atggcggtgc tgcacccgga
     2101 cagcgagccg atgctgcagg ccccgcaagg cgcgcgcgtc gtgatgatcg gcggcgcgcc
     2161 gctcggcc
//
LOCUS       DASUCY010000171         3221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_405241, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000171 DASUCY010000000
VERSION     DASUCY010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405241"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            355..2442
                     /locus_tag="VFV25_03325"
     CDS             355..2442
                     /locus_tag="VFV25_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEY1392375.1"
                     /translation="MAVQQQQISMAPDPAHLESIVRSHERCAALGLSRIERPDHTPLI
                     RSDLTVARDRNQRLLVHAAPIMELLLEQILATQSMVVLTDTQGTVLHSVGHEDFLQRA
                     SKIALAPGVNWSESCKGTNAIGTALINETPTLVHADEHFMHANGFLTCSAAPILDPRG
                     NMLGVLDVTGDQRSYHHHTMGLVKMSARMIENHWLSDDFRHAMRLHFHSRPEFIGTLM
                     EGIVAVGRDGRFVGANRSALDQLAMSGAALRMNSVVSLFGIPVAAIIDHFRSPMAMPL
                     LLNLSNGQKVHVRARFNWPTWHALGSAGLEDEAAAPELVPTASPAPVSETTRTWKAPV
                     ATTPPQLSGLRYLNTGDAQMEAVISKVRRVLNRDISIMVLGETGTGKELLARAIHHDS
                     ERARQPFVAVNCASIPETLIESELFGYEEGAFTGARRKGAVGKILQANGGTLFLDEIG
                     DMPLQLQARLLRVLQERQVTPLGSGKSVPVDVAIISATHRNLREMIAAQAFREDLYYR
                     LNALVVRLPALRERSDLMTIVQKILRVECNGVLPELASDVLPLLQAYQWPGNIRQLAS
                     VLRTAAVMAAGELQITRAHLSDDFIEDAERASLGAAGTPGPAVSGFAAPVIEPVARVA
                     ALDGEGAATAPGPTAEVPTDAPASAPTTLEELELQTIRRAVEEAGGNISVASKRLGIS
                     RNTIYRKLRWQQH"
     gene            2650..2799
                     /gene="pqqA"
                     /locus_tag="VFV25_03330"
     CDS             2650..2799
                     /gene="pqqA"
                     /locus_tag="VFV25_03330"
                     /inference="COORDINATES: protein motif:HMM:NF019654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone precursor peptide PqqA"
                     /protein_id="HEY1392376.1"
                     /translation="MRIADLPCRGTALLPWPLNPERRLTMLWTKPQYQDLRFGFEITM
                     YISAR"
     gene            2863..>3221
                     /locus_tag="VFV25_03335"
     CDS             2863..>3221
                     /locus_tag="VFV25_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017423360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY1392377.1"
                     /translation="MKILVLGSGAGGGFPQWNCNCALCAGLRSGTTRSTPRTQSSIAI
                     SCDGEQWVLLNASPDVGQQIRSNAALHPRGGPRDTPIKAVILMDSQVDHVTGLLSLRE
                     GPPIELHCTPCVFEDLTT"
BASE COUNT          575 a         1138 c          999 g          509 t
ORIGIN      
        1 ggctgatgca cagcgcggcg gcgcggatga tgtggtgtct cctcatgtcg gtctccatcg
       61 ggacggtgtt gcagtcgccc acttgactgc aagcgatgtg ccacacggca aagcgactca
      121 tccccggcct tcgcttgcgt aatagccata cccagtcgga ccgggttgtt ccaaaatagg
      181 acagccgtgg cttgagattt gtcaaattcg gccttttcgc catggcaaac cgcaatggct
      241 ttgctccagc ctcgcccata acatcctcca tgcctagttt ttgctaggac gacaggaagg
      301 aagacaacaa gagaacggct gggcttcgca ggatcgaagc caaaggagac aagcatggcc
      361 gtccagcagc agcagatcag catggcgccg gaccccgccc atctggaaag catcgtgcgc
      421 tcgcatgagc gctgcgcagc actcgggctc agtcgcatcg agcggccgga ccatacgccg
      481 ctgattcgct ccgacctcac ggtcgcgcgc gatcgcaacc agcgcctgct ggtacacgcc
      541 gcgccgatca tggagctgct gctggagcag atcctcgcca cgcagagcat ggtcgtgctc
      601 accgacacgc agggcacggt gctgcattcg gtgggccacg aggacttcct gcagcgcgcc
      661 tccaagatcg cgctcgcgcc gggcgtcaac tggtcggagt cctgcaaggg caccaatgcg
      721 atcggcaccg ccctcatcaa cgagacgccg acgctggtgc atgccgacga gcatttcatg
      781 catgccaacg gcttcctcac ctgctcggcc gcgcccatcc tcgatccacg cggcaacatg
      841 ctcggtgtgc tcgacgtgac cggcgaccag cgctcctacc accatcacac gatgggcctg
      901 gtcaagatgt cggcccgcat gatcgagaac cactggctga gcgacgactt ccgccacgcc
      961 atgcggctgc acttccacag ccgccccgaa ttcatcggca cgctgatgga aggcatcgtc
     1021 gcggtgggcc gcgacggccg cttcgtcggc gccaaccgca gcgcgctcga ccagctggcg
     1081 atgagcggcg cggccctgcg catgaacagc gtggtcagcc tgttcggcat cccggtggcg
     1141 gccatcatcg accatttccg ctcgcccatg gccatgccgc tgctgctcaa tctgagcaac
     1201 ggccagaagg tgcacgtgcg ggcgcgcttc aactggccga cttggcacgc gctgggcagc
     1261 gccgggctcg aagacgaggc cgcggcaccc gagctggtgc cgaccgccag cccggcgccg
     1321 gtcagcgaaa ccaccaggac ctggaaggcg ccggtcgcaa cgacgccgcc tcagctctcg
     1381 gggctgcgct atctgaacac cggcgacgcg cagatggagg ccgtcatctc caaggtgcgc
     1441 cgcgtgctca accgcgacat ctcgatcatg gtgctgggcg agaccggcac cggcaaggaa
     1501 ctgctggccc gcgcgataca ccacgactcc gagcgcgcgc ggcagccctt cgtcgcggtc
     1561 aactgcgcgt cgatccccga gacgctgatc gaatccgagc tgttcggcta cgaggaaggc
     1621 gccttcaccg gcgcgcgccg caagggcgcg gtcggcaaga tcctgcaggc caacggcggc
     1681 acgctcttcc tcgacgagat cggcgacatg ccgctgcagt tgcaggcgcg attgctgcgc
     1741 gtgctgcagg agcgccaggt cacgccgctg ggcagcggca agtcggtgcc tgtggatgtc
     1801 gccatcatca gcgccaccca ccgcaacctg cgcgagatga tcgccgccca ggccttccgc
     1861 gaagacctct actaccggct caacgcgctg gtggtacggc tgccggcgct gcgcgagcgc
     1921 agcgatctga tgaccatcgt gcagaagatc ctgcgcgtcg aatgcaacgg cgtgctgccg
     1981 gagctcgcct cggatgtcct gccgctgctg caggcctatc aatggcccgg caacatccgc
     2041 cagctggcca gcgtgctgcg cacggccgcg gtgatggccg cgggcgagtt gcagatcacg
     2101 cgcgcccatc tgtccgacga tttcatcgag gacgccgagc gcgcgtcgct cggcgcagcc
     2161 ggcacgccgg gcccggccgt gagcggcttc gcggcaccgg tcatcgagcc cgtggcacga
     2221 gtcgccgcgc ttgacggcga aggggccgcc acagcgcccg gcccgacagc cgaagtgccg
     2281 actgacgctc ccgccagcgc gcccaccacg ctggaagaac ttgaactgca gaccatacgc
     2341 cgcgcggtgg aagaggccgg cggcaacatc tcagtcgctt ccaagcgcct gggcatcagc
     2401 cgcaacacca tctatcgcaa gctgcgctgg cagcagcact agtaccgagt tcgtaagagc
     2461 ctggagccgg gtattccctg ttccaggctc tgtgccaagc cctggcacag ctgctccagc
     2521 acgtgacagt gcgcgacgcg ccacgcagcg cgccgacctc gcactgcctc accgttcgcc
     2581 tcgcaccgaa cgatcgcgcc acctcactcg cgagtttccc taggtttcgg cctcaactga
     2641 gcaggcggca tgcgtattgc agatttacct tgccgcggga cagcgctgct gccctggccc
     2701 ctcaaccctg aaaggagact caccatgctg tggaccaagc cccaatacca ggacctgcgc
     2761 ttcggtttcg agatcacgat gtacatctcg gcacgctgat gacgcgaagg gcagaggcga
     2821 gtgcctctgc ccgcaacaaa ttcctgtctc tctcgccatc ccatgaagat actcgtgctg
     2881 ggctccggag ccggcggcgg ctttccgcag tggaattgca actgcgcgct gtgcgccggg
     2941 ttgcgcagcg gcaccacgcg ctcgacgccg cgcacgcaga gctcgatcgc catttcttgc
     3001 gacggcgagc aatgggtgct gctcaatgcc tcgccggacg tcggccagca gatccgcagc
     3061 aatgccgccc tgcacccgcg cggcggcccc agggataccc cgatcaaggc ggtgatcctg
     3121 atggactccc aggtcgacca tgtcaccggc ctgctgagcc tgcgcgaagg ccctcccatc
     3181 gaactgcact gcacgccctg cgtgttcgag gatctcacga c
//
LOCUS       DASUCY010000172         2826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1561826, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000172 DASUCY010000000
VERSION     DASUCY010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1561826"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1410
                     /gene="paaZ"
                     /locus_tag="VFV25_03340"
     CDS             <1..1410
                     /gene="paaZ"
                     /locus_tag="VFV25_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HEY1392378.1"
                     /translation="GGTGDLLDRLEEPDVVTFTGSADTAARLRTNRNIVARSIPFNAE
                     ADSLNCAILAPDVSPDDEEFDLFVKEVAREMTVKAGQKCTAIRRAIVPRKHIDAVAAK
                     LRERLAKTVVGDPSVEGVRMGALASKAQQADVAERVAMLAKGNELVFGARDGFAPVGK
                     ETAQGAFFSPTLLLCRDALDNAAVHDVEAFGPVSTLMPYEDIDEAVALAARGKGSLVG
                     TLVTHDPAVAAKVIPMAAAYHGRLLVLDREAAAESTGHGSPLPPLKHGGPGRAGGGEE
                     LGGLRAVKHYLQRAALQGSPTMLAAVTGEHVRGAKLREGDVHPFRKHFEDLQVGDSLL
                     THRRTVSEADIVAFGGLSGDYFYMHFDEIAAKQSPFGKRIAHGYFVLSAAAGLFVSPA
                     PGPVLANYGLDTLRFVKPVGIGDTIRARLTCKRKIDRNKKDAQGVGQGVVAWDVEVTN
                     QNDELVASYDILTLVSKRS"
     gene            1559..1891
                     /locus_tag="VFV25_03345"
     CDS             1559..1891
                     /locus_tag="VFV25_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392379.1"
                     /translation="MRIFRPNRVVLGAIAALSLTLAGCYVVPADPYYQSSEVVTVAPP
                     PAQVEVVGVAPYPGYIWIGGYWGWSGYRHVWYPGYWSAPRPGYRWYPHNWDRSGNGWR
                     ESRGHWGR"
     gene            complement(1908..2630)
                     /locus_tag="VFV25_03350"
     CDS             complement(1908..2630)
                     /locus_tag="VFV25_03350"
                     /inference="COORDINATES: protein motif:HMM:NF025433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LssY C-terminal domain-containing protein"
                     /protein_id="HEY1392380.1"
                     /translation="MSRPALRIAALLAALALGAALVYGLTAYEFVPSVWRFVERRHPA
                     LDRMGSCAFTKVGIPGDPLNLAFIGSEEALQKAMLRAGWQPADPITLKSSLRIAVDSV
                     VHRPYLDAPVSDLYINGKKQDLAFEQAAAGDPSKRHHVRFWAAAQRDALDRPLWMGAA
                     TFDSGIGLSHRTGQITHHIAPEVDAERDKLLKDLQALGSLSFRWIDEFQPQRSGKNGG
                     GDRYVTDGRLAVIVAGEATSHP"
BASE COUNT          448 a          942 c          971 g          465 t
ORIGIN      
        1 ggcggcaccg gcgacctgct cgatcgcctg gaggagccgg atgtcgtcac cttcaccggt
       61 tcggccgaca cggctgccag gctgcgcacc aaccgcaaca tcgtcgcacg ttccatcccc
      121 ttcaacgccg aggccgattc tctcaactgc gcgatcctcg cgcccgacgt ttcgcccgac
      181 gacgaggagt tcgatctctt cgtcaaggaa gtggcacgcg agatgaccgt caaggccggg
      241 cagaaatgca cggccatccg ccgcgccatc gttccgcgca agcacatcga cgcggtggcg
      301 gccaagctgc gcgagcggct ggcaaagacg gtggtgggcg atccgtcggt ggagggcgtg
      361 cgcatggggg cactggcttc caaggcccag caggccgatg tggccgagcg cgtggcgatg
      421 ctcgccaagg gcaacgagct ggtcttcggc gcgcgcgacg gctttgcgcc ggtgggcaag
      481 gagacagcgc agggcgcctt cttctcgccc acgctgctgc tgtgccgcga tgcgctggac
      541 aacgcggccg ttcacgacgt ggaggccttc ggcccggtca gcacgctgat gccctacgaa
      601 gacatcgacg aagccgtcgc gctggcggcg cgcggcaagg gcagcctggt cggcaccttg
      661 gtgacgcatg acccggccgt ggccgccaag gtgatcccta tggccgcggc ctatcacggc
      721 cgcttgctgg tgctggaccg cgaggcggcg gccgagtcca ccggccacgg ctcgccgctg
      781 ccgccgctca agcatggcgg cccgggccgc gccggcggcg gcgaggagct gggcgggctg
      841 cgcgccgtca agcactacct gcagcgtgcc gcgctgcagg gctcgcccac catgcttgcg
      901 gcagtgaccg gcgagcatgt gcgcggcgcc aagctgcgcg agggcgacgt gcatcccttc
      961 cgcaagcatt tcgaggatct gcaggtgggc gattcgctgc tcacccaccg ccgcaccgtc
     1021 agcgaggccg atatcgtcgc cttcggcggg ctgtcgggcg actatttcta catgcatttc
     1081 gacgagatcg ccgcgaagca atctcccttc ggcaagcgca tcgcgcatgg ctatttcgtg
     1141 ctgtcggcag cggccgggct cttcgtgtcg ccggcgccag gcccggtgct ggccaactac
     1201 ggcctggaca cgctgcgctt cgtcaagccg gtgggcatag gcgacacgat cagggcgcgc
     1261 ctgacctgca agcgcaagat cgaccgcaac aagaaggatg cccagggcgt cgggcagggc
     1321 gtggtggcct gggacgtgga agtgacgaac cagaacgacg aactggtggc gtcctacgac
     1381 atcctgacat tggtgagcaa gcgcagctga tccccatgca gccggcgcat ggctgtgaag
     1441 ccggtgcaaa gaagtgtgat cccgcccgcc cgggggcggg attgtcgctg cgtcgcgtca
     1501 tcggtttgag ccccgccagc gttaaggggg taactcttgg catcgagtcg aggattacat
     1561 gcgcatcttt cgtcccaacc gcgtcgttct aggcgccatc gcagccctgt cgctcacgct
     1621 tgcaggctgc tatgtagtgc ctgctgaccc ttactaccag agcagcgagg tggtgacggt
     1681 tgcaccgccg ccggcccagg tcgaggtggt gggcgttgcg ccctatcccg gctacatctg
     1741 gatcggcggc tattggggct ggtcgggcta ccgccacgtc tggtaccccg gctattggtc
     1801 ggcgccgcgg cccggctacc gctggtatcc ccacaactgg gaccgctccg gcaacggctg
     1861 gcgcgaatcg cgcggccact ggggccgctg acccggtgtt tgtgtgatca aggatggctc
     1921 gtcgcttcgc cggcgacgat cacggccagc cgcccgtcgg tgacatagcg gtcgccgccg
     1981 ccgttcttgc cgctgcgctg cggctggaac tcgtcgatcc agcggaagct caggctgccc
     2041 agggcctgca agtccttgag cagcttgtcg cgctcggcat cgacttcggg cgcgatgtgg
     2101 tgcgtgatct ggcccgtcct gtggctcagg cctatgcctg aatcgaaagt cgccgcgccc
     2161 atccacaagg ggcggtccag ggcatcgcgc tgcgctgcgg cccagaacct gacgtgatgc
     2221 cgcttggacg gatcgcccgc cgcggcctgc tcgaaggcga ggtcctgctt cttgccgttg
     2281 atatacaaat cgctcaccgg cgcgtcgaga taaggcctgt gcacgacgct gtcgaccgcg
     2341 atgcgcagcg aactcttcag cgtgatggga tcggccggct gccagccggc acgcagcatc
     2401 gccttctgca gggcctcctc gctgccgatg aaggccaggt tcagcggatc accggggatg
     2461 ccgaccttgg tgaaggcgca gctgcccatg cggtccaggg ccggatgccg gcgctccaca
     2521 aagcgccaca ccgaaggcac gaactcgtaa gcggtcagcc cgtaaacgag cgccgcgccc
     2581 agggcgagcg cggcgagcag ggcggcgatg cgcagggccg gtcggctcat gccgtgcccc
     2641 cgcagatcgt cgcccgcgcg gtcatcagaa gctgtagccg acgaaaagat cgctggcgat
     2701 gccttggctc ttcagctggt cttccaccgt gaccgtgctc gaggacacgg tggccttggc
     2761 cttcagcggc agataggtca gcgagaagcc cagcgaccag gtgtcgttga agcgatagtt
     2821 gaaacc
//
LOCUS       DASUCY010000173         3578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1160426, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000173 DASUCY010000000
VERSION     DASUCY010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160426"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VFV25_03355"
     CDS             <1..451
                     /locus_tag="VFV25_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY1392381.1"
                     /translation="AVLTTDAVYQAFYDDSTARGFLHSHSYTGNPLACRAALAVLDVF
                     ERSPVLERNRAAAARLNELNTALAAHPRVRHARQLGMIWAYDIDTQDPGFAARYRQAA
                     LDQGLLLRPIGNTLYFMPPYVLDEEALQLLAAGSLKALDVALAGGEA"
     gene            448..1125
                     /gene="bioD"
                     /locus_tag="VFV25_03360"
     CDS             448..1125
                     /gene="bioD"
                     /locus_tag="VFV25_03360"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HEY1392382.1"
                     /translation="MSPAWFVTGTDTGIGKTRASCALLHALRRAGHAAVGMKPVAAGT
                     EPGADGSRINEDVQALREASALRVPAELDNPVLLDAATSPHIAARRARRCIELAPLAA
                     ACAALRERAGAVVVEGAGGFCVPLSDTETGADLATLLGLPVILVVGLRLGCLNHALLT
                     QEAIAARGLRLAGWVANRIDPHMQAQDENLEFLRTRLRAPMLADWPWAPGVSPRDLHI
                     ELPAFHA"
     gene            1118..2284
                     /gene="bioF"
                     /locus_tag="VFV25_03365"
     CDS             1118..2284
                     /gene="bioF"
                     /locus_tag="VFV25_03365"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEY1392383.1"
                     /translation="MPEPSEVFAPALQALEQQHLLRRRRVAQAADGAYCIVNGERRLS
                     FAGNDYLGLSQHPLLAEAAQRAIAEYGVGATASPLVCGHSPAHEALEQELARFVGLPR
                     ALYFYAGYAANAGAVPALVGAGDAIFSDALNHACLIDGARLSRAAIHVYPHLDLAALD
                     AALAISTARNKLVITDAVFSMDGDLAPLSELLRLCERHDAWLMVDDAHGFGVLGEGGR
                     GSLSHWKVSSPRLIYMATLGKAAGVAGAFFAGAPAVVEWLLQRARTYMFATAAPALIA
                     QTLRASLKLIEAEGWRRERLAELRARLNAGLAGQPWKLLPSDTAIQPLVLGDSARALD
                     VMNGLDARGLWVPAIRPPTVPAGTARLRISLTAAHEPADVDRLTVALGELAAAG"
     gene            complement(2297..>3578)
                     /gene="miaB"
                     /locus_tag="VFV25_03370"
     CDS             complement(2297..>3578)
                     /gene="miaB"
                     /locus_tag="VFV25_03370"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEY1392384.1"
                     /translation="KAAEGYEATDDVEQADLILFNTCSVREKAQEKVFSDLGRIKHLK
                     ARGVKIGVGGCVASQEGAAIIERAPYVDVVFGPQTLHRLPEMLAKRDALNRPQVDISF
                     PEIEKFDHLPPARVEGATAFVSIMEGCSKYCSYCVVPYTRGEEVSRPFEDVLTEVAGL
                     ADQGVKEVTLLGQNVNAYRGKMGDSDEIADFALLIEYVAEIPGIERIRYTTSHPNEFT
                     QRLIDVYAKVPQLVNHLHLPVQHGSDRILMAMKRGYTALEYKSTIRKLRAVRPDISLS
                     SDFIVGFPGETEEDFAKMMKLIDDVGYDASFSFVFSPRPGTPAAALHDDTPQAVKLAR
                     LQQLQARIEENVQRISASRVGTLQRILVEGPSRRPGPDGRPELMGRTECNRIVNFAGA
                     ARLIGQMIAVRITEAYPHSLRGELPTRETTALAA"
BASE COUNT          496 a         1255 c         1218 g          609 t
ORIGIN      
        1 cgctgtgctg acgaccgatg ccgtctacca ggccttctac gacgacagca cggcgcgcgg
       61 cttcctgcat tcgcattcct acaccggcaa tcccctggcc tgccgtgcgg cactggccgt
      121 gctggacgtc ttcgagcgct cgccggtgct ggagcgcaac cgcgccgccg cagcgcgctt
      181 gaacgagttg aacaccgcgc tcgccgccca tccgcgcgtg cggcatgccc gccaactcgg
      241 gatgatctgg gcctacgata tcgacacgca ggatcccggc ttcgccgccc gctatcggca
      301 ggcagctttg gaccagggcc tgctgctgcg cccgatcggc aataccctct acttcatgcc
      361 gccctatgtg ctcgatgaag aagccctgca gctactggcc gctggcagcc tgaaggcgct
      421 ggacgttgca ctggctggag gcgaggcttg agtcctgcct ggttcgtcac cggcacggac
      481 accggcatcg gcaagacccg cgccagttgc gcgctgctcc acgctctacg ccgtgcgggc
      541 catgccgccg tcggcatgaa gccggtcgcg gcaggcaccg agcccggcgc cgacggatcg
      601 cgtatcaacg aggacgtgca ggccctgcgc gaggcgagcg cactgcgcgt gccggccgag
      661 ctggacaacc cggtgctgct ggatgccgcg acctcgccgc atatcgccgc gcgccgcgct
      721 aggcgctgca tcgaactggc gccgctcgcg gctgcctgcg cagccttgcg cgagcgcgcg
      781 ggggcggttg tggtcgaggg ggcagggggc ttttgcgtgc ccttgtcgga taccgagacc
      841 ggcgccgatc tggcgacgct gctcggcttg cccgtgatcc tggtcgtggg cttgaggctg
      901 ggctgcctca accatgcgct gctgacgcag gaggccattg ccgcgcgcgg cctgcgcttg
      961 gccggctggg tggccaaccg catcgatcca catatgcagg cgcaggacga gaatcttgaa
     1021 ttcctccgaa cccggctgcg cgcgccgatg ctggccgact ggccctgggc accgggcgtg
     1081 agcccgcgcg acctgcacat cgaactgccc gccttccatg cctgaacctt ctgaagtctt
     1141 cgcgcctgcg ctgcaggccc tggagcagca gcatctgctg cgccgccgcc gcgtggcgca
     1201 agccgccgac ggcgcgtatt gcatcgtgaa cggcgaacgg cgcctgtcct tcgccggcaa
     1261 cgactatctc ggcctttcgc agcatcccct gctggccgag gcagcgcagc gtgcgatcgc
     1321 cgaatatggc gtcggggcca ccgcatcgcc gctggtgtgc ggccacagcc ccgcacacga
     1381 ggcgctggag caggagcttg cgcgcttcgt cggcctgccc agggcgctct atttctacgc
     1441 cggttacgcg gccaatgccg gcgccgtgcc ggccctggtg ggggcgggtg acgcgatctt
     1501 ctcggacgcg ctcaaccatg cctgcctgat cgacggggcg cggctgtcgc gcgcggcgat
     1561 ccatgtctat ccgcatctgg atctagccgc cctggatgcg gcgctcgcca tcagcactgc
     1621 acgcaacaag ctcgtgatca ccgatgccgt cttcagcatg gacggcgacc tggccccgct
     1681 gtccgagctg ctgcgcctgt gcgaacgcca cgacgcatgg ctgatggtcg acgatgccca
     1741 cggcttcggc gtgctgggcg agggtggccg cggcagcctt tcgcattgga aggtgagctc
     1801 gccgcgtctc atctacatgg cgacgctggg caaggcggcc ggcgtggccg gtgccttctt
     1861 cgccggagcg ccggcggtcg tcgaatggct gctgcagcgc gcccgcacct atatgttcgc
     1921 caccgccgcg ccggcgctga ttgcgcagac gctgagggcg agcctcaagc tcatcgaggc
     1981 cgagggctgg cggcgcgagc gcctcgcgga gctgcgtgcc cgcttgaacg ccggcctggc
     2041 cggccagccc tggaagctgc tgccttccga cacggcgatc cagcccttgg tgcttggcga
     2101 cagtgcgcgc gccctggacg tgatgaacgg cctcgatgcg cgcggcctgt gggtgccggc
     2161 catacgcccg cccaccgttc cggcgggcac ggcgcggctg cgcatctcgc tgaccgcggc
     2221 gcatgagccg gccgacgtgg accggctcac cgtggccctg ggtgaattgg ccgcagcagg
     2281 ctgaagccgc agcgactcag gccgccagcg ccgtggtctc gcgcgtcggc agctcgccgc
     2341 gcagcgagtg cggataggcc tcggtgatgc gcacagctat catctggccg atcagccgcg
     2401 cggcacccgc gaagttgacg atgcggttgc actcggtgcg gcccatcagc tccggcctgc
     2461 catcggggcc ggggcggcgc gacggccctt cgaccaggat gcgctgcagc gtgccgacgc
     2521 ggctcgcgct gatgcgctgc acgttctcct cgatacgcgc ctgcagctgc tgcagccgcg
     2581 cgagcttcac ggcctgcggc gtgtcgtcgt gcagcgcggc ggccggcgtg ccggggcgcg
     2641 ggctgaacac gaagctgaag ctggcgtcgt aacccacgtc gtcgatcagc ttcatcatct
     2701 tggcgaagtc ttcttccgtc tcgccgggga agccgacgat gaagtcgctc gacaggctga
     2761 tgtccggccg caccgcacgc agcttgcgga tcgtgctctt gtattcgagc gccgtgtagc
     2821 cgcgcttcat cgccatcagg atgcggtcgg agccatgctg caccggcagg tgcagatgat
     2881 tcaccagttg cggcaccttg gcgtagacat cgatcagacg ctgcgtgaac tcgttggggt
     2941 gcgaggtggt gtagcggatg cgctcgatgc ccgggatctc ggccacgtat tcgatcagca
     3001 gcgcgaagtc ggcgatctca tccgagtcac ccatcttgcc gcggtaggcg ttgacgttct
     3061 ggcccagcag cgtcacttcc ttcacgccct ggtcggccag gcccgccact tcggtcagca
     3121 cgtcctcgaa ggggcggctc acctcttcgc cgcgggtgta aggcaccacg cagtagctgc
     3181 agtacttgga gcagccttcc atgatcgaca cgaaggcggt tgcgccttcg acgcgcgccg
     3241 gcggcaggtg gtcgaacttc tcgatctccg ggaagctgat gtccacctgc gggcggttca
     3301 gcgcatcgcg cttggccagc atctcgggca ggcggtgcag cgtctgcgga ccgaacacca
     3361 cgtccacata gggcgcgcgc tcgatgatgg ctgcgccctc ctggctcgcc acgcagccgc
     3421 ccacgccgat cttcacgccg cgggccttca ggtgcttgat gcgcccgagg tcggagaaca
     3481 ccttctcttg cgccttctcg cgcaccgagc aggtgttgaa caggatgagg tcggcctgct
     3541 cgacgtcgtc ggtggcctcg tagccctcgg cagccttc
//
LOCUS       DASUCY010000174         4308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_244834, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000174 DASUCY010000000
VERSION     DASUCY010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4308
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244834"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..615
                     /gene="gspG"
                     /locus_tag="VFV25_03375"
     CDS             175..615
                     /gene="gspG"
                     /locus_tag="VFV25_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HEY1392385.1"
                     /translation="MTLQLSLPRRRARGFTLIEVMVVLVIIGVMAALIAPSVLRRVDE
                     ARVTAARTDINTLMQALKLYKLDNQRFPTGEQGLQALVAKPTTGPIPSNWKPYLEKLP
                     VDPWGNPYQYANPGVKGEVDVFSYGADGKPGGEGVDADIGSWQQ"
     gene            587..1093
                     /locus_tag="VFV25_03380"
     CDS             587..1093
                     /locus_tag="VFV25_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY1392386.1"
                     /translation="MPTSAPGSNSFPLMQRRAPARAGGFTLIEVLIVLVIIALASSLV
                     ALALRDPASTQLEREASRLAALLEAERSEARSAGLAVLWVPGQNNRDGTDFQFVGLPT
                     GQTPPAQHWLDPDVQAEVVGARTVTLGPEPIIGAQRILLHLRDQRLALVTDGLGPFKV
                     ESMEASPP"
     gene            1090..1473
                     /gene="gspI"
                     /locus_tag="VFV25_03385"
     CDS             1090..1473
                     /gene="gspI"
                     /locus_tag="VFV25_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HEY1392387.1"
                     /translation="MKLATNKRQPAHGLTLIEVLVALAIVAITLAAGIKAAGALMGNA
                     QRLDEVTAAQWCADNQLTELRLSQQFPGIGDSDFGCQQLGRSYAGKLMTRPTLNPNFR
                     RVDAQISNDEGQPVLTLTTILPRYP"
     gene            1470..2120
                     /locus_tag="VFV25_03390"
     CDS             1470..2120
                     /locus_tag="VFV25_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEY1392388.1"
                     /translation="MKRSRLTAGFTLVEVLVALAILAIMAALAWRGVDGIVRARDGSQ
                     SRLEQTLRLQTVMAQWEADLSQAIDTHVVPPLMFDGATLRMTRRTPDGVQVVAWSLKG
                     GTWQRWAGPVATRSGALQDAWFQSQQLLGSEPGQLKLLPGVGSWQLYYYIGNAWANAQ
                     SSGNQVPNAAAGSASAPILVGTHTGLPDGVRSVLNFGEGSGLSGNLTRSVKLVHPS"
     gene            2117..3151
                     /gene="gspK"
                     /locus_tag="VFV25_03395"
     CDS             2117..3151
                     /gene="gspK"
                     /locus_tag="VFV25_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HEY1392389.1"
                     /translation="MKAALYKSPASPARQRGAALLTALIIVTLVATLASAMVWQQWRA
                     VQVEAAERSRTQSYWVLSGALDWARLILREAAQRKNRASGDNLGEAWAAPLAEARLSS
                     FLAADKDNNASDDNAGPEAFLSGQITDEQSRYNLHNLVANGAVQPKEMAALQRLCEIL
                     GLDSSVASRIAQGLQPALTPAALAASAAGANSASPEAMLLPPSVADLGWLGIDAASIA
                     ALRPYVTLLPRNTPVNLNTAPKEVIAAVIPVDLASADRLVQYRQRSPFTDLGQVQAQL
                     GSSDGKGQVQLDPERVDYRTSFFEVRGRLRLDDRVVEERSLVERRGIAVVVLRRERIA
                     GIESASASRP"
     gene            3287..>4308
                     /gene="gspL"
                     /locus_tag="VFV25_03400"
     CDS             3287..>4308
                     /gene="gspL"
                     /locus_tag="VFV25_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HEY1392390.1"
                     /translation="MSLLVVQIPPRPRLRSAAPAGADDGARASTEFAYVLSSDGLASQ
                     RQGLCAPSLLPKADTVVAVLAENDVSWHRVNLPRAPANRLRAALSGLLEEALLDETEH
                     VHLALAPQPKAGEPTWIAACDHRWLATELATLERAGVRVDRVAPSAWPDEPSSGHFHE
                     LSAEAGEQLNVALTWSHGNGVATWPLQGGLAHALLPQPLPETAGFTATPAVAAPAERW
                     LGATVAVVSPGERALAAARSLWNLRQFGLAPRHRGADLLRARWRQFLSPAWRPARVGL
                     LVLVLMQIVGLNLWAWQQKAQIASRRAAMNALLTTTYPQVRTVYAAPEQMQKETELLR
                     TAAGQPG"
BASE COUNT          685 a         1570 c         1437 g          616 t
ORIGIN      
        1 agacgcaggc cccagaaggc caagctggcc gcgatcgcgg cccacacgat gaaagcactc
       61 aatcgggcag ccatgggcgg cgattatgat ggaacgttgg cttcattcgg cgcagtcgag
      121 agccggtaca cagcctgaca caagcccttt acgcgacgag tacaagatca agccatgacg
      181 ctacaactta gcttgccgcg ccgccgtgcg cgcggtttca ccctgatcga agtaatggta
      241 gtgctggtca tcatcggcgt gatggccgcg ctgatcgccc ccagcgtgct caggcgcgtc
      301 gacgaggcac gcgtcactgc cgcgcgcacc gacatcaaca ccctgatgca ggcgctcaag
      361 ctgtacaagc tggacaacca gcgcttcccc accggcgagc agggcctgca ggcgctggta
      421 gccaagccca cgacgggccc catcccgtcg aactggaagc cctatctgga gaagctgccc
      481 gtcgatccct ggggcaatcc ttaccaatac gccaatccgg gcgtgaaagg cgaggtcgat
      541 gtgttcagct acggtgcgga cggcaagccg ggcggtgaag gcgtcgatgc cgacatcggc
      601 tcctggcagc aatagcttcc cgctgatgca gcgccgcgct cctgcgcggg ccggcggttt
      661 cacgctgatc gaggtgctga tcgtgctggt catcatcgcc ctggcctcct cgctcgtggc
      721 actggccctt cgcgaccccg cgagcacgca gctcgagcgt gaagcctcgc gcctggcggc
      781 cctgctcgaa gccgagcgca gcgaggcgcg ttcggccgga ctggccgtgc tgtgggtgcc
      841 cggccagaac aacagggatg gcaccgactt ccaattcgtc ggcctgccta ccggccagac
      901 cccgcctgct caacattggc tggacccgga cgtccaggcc gaagtggtgg gcgcgcgcac
      961 cgtgacgctc ggccccgaac ccatcatcgg cgcccagcgc atcctgctgc acttgcgcga
     1021 ccagcgcctg gcgctcgtga ccgacgggct cggccccttc aaggtggaaa gcatggaggc
     1081 cagcccgcca tgaagctcgc cacgaataag cggcagccgg cacatgggct gacgctgatc
     1141 gaggtgctgg tcgcgctggc catcgtcgcg atcacgctgg ccgccggcat caaggccgcc
     1201 ggcgcgctga tgggcaatgc ccagcgcctg gacgaagtga cggcggcgca gtggtgcgcc
     1261 gacaaccagc tgaccgagct acgcctgtcg cagcagttcc ccggcatcgg cgacagcgat
     1321 ttcggctgcc agcagctggg ccgcagctac gccggcaagc tcatgacccg gcccacgctc
     1381 aatcccaatt tccgccgcgt cgacgcccag atcagcaatg acgaaggcca gccggtgctg
     1441 acgctgacca ccatcctgcc gaggtatccg tgaagcgaag cagactcacg gcgggcttca
     1501 cgctggtcga ggtgctggtg gcgctcgcca tcctggccat catggccgcg cttgcctggc
     1561 gcggcgtgga cgggatcgtg cgcgcgcgcg acggctcgca gtcgcggctg gagcagaccc
     1621 tgcgcctgca gaccgtgatg gcgcaatggg aggccgatct gtcgcaggcg atcgacacgc
     1681 atgtggtgcc gccgctgatg ttcgacggcg ccacgctgcg catgacgcgg cgcacgcccg
     1741 acggcgtgca ggtcgtggcc tggtcgctca agggcgggac ctggcagcgc tgggccggcc
     1801 cggtggcgac gcgcagcggc gcgctgcagg acgcctggtt ccagtcgcag cagctgctgg
     1861 gcagcgagcc cggccagctc aagctcctgc cgggcgtggg cagctggcag ctctactact
     1921 acatcggcaa tgcctgggcc aatgcgcagt ccagcggcaa ccaggtgccg aatgcggccg
     1981 ccggctcggc ctcggcgccc atcctcgtcg gcacccatac cggcctgccc gacggcgtgc
     2041 gcagcgtgct caacttcggc gaaggcagcg ggctcagcgg caacctgacc cgcagcgtga
     2101 agctggtgca tccatcatga aggcagcgct gtacaagtcc cccgcatcgc ctgcgcgcca
     2161 gcgcggcgca gccctgctca ccgccctcat catcgtgacc ctggtggcca ccctggcctc
     2221 ggccatggtg tggcagcaat ggcgcgcggt gcaggtcgag gcggcagagc gcagccgcac
     2281 gcagtcctat tgggtcctga gtggcgcgct cgactgggcc cgcctgatcc tgcgcgaggc
     2341 cgcacagcgc aagaaccgcg cgagcggcga caacctgggc gaggcctggg ccgcaccgct
     2401 ggccgaggcg cggctgtcga gcttcctcgc cgccgacaag gacaacaacg ccagcgacga
     2461 caacgccggc cccgaggcct tcctgtcggg ccagatcacc gacgagcagt cgcgctacaa
     2521 cctgcacaac ctggtcgcca acggcgccgt gcagccgaag gagatggccg cgctgcagcg
     2581 gctgtgcgag atcctgggtc tggacagcag cgttgccagc cgcatcgccc agggcctgca
     2641 gccggccctg acgcccgcgg ccctggccgc ttcggcggcg ggcgccaaca gcgcctcccc
     2701 cgaagcgatg ctgctgccgc cttcggtggc cgacctcggc tggctcggca tcgatgccgc
     2761 ctcgatcgcg gcgctgcggc cctatgtcac gctgctgccc cgcaatacgc cggtcaatct
     2821 gaacacggcg cccaaggaag tgatcgccgc cgtgatcccc gtcgacctcg ccagcgccga
     2881 ccgcctggtg caataccgcc agcgcagtcc cttcaccgat ctcggccagg tgcaggccca
     2941 gctcggcagc agcgacggca agggccaggt ccagctcgat cccgagcgcg tcgactaccg
     3001 aaccagcttc ttcgaagtgc gcgggcggct gcggctggac gatcgcgtgg tcgaggagcg
     3061 ctcgctggtc gagcggcgcg gcatcgcggt cgtggtgctg cgccgcgagc gcatcgctgg
     3121 catagagagt gccagtgcgt cccggccctg acggcgcgaa acgatccatg cgctactgaa
     3181 aagatactta catgccgcaa aactacctgg tgaaaacact gaaatcctag gttattcgac
     3241 agcaagcgcc ccctacaatg ccccgctccc ctaccccgac acccacatga gcctgctcgt
     3301 cgtccagata ccgccgcgcc cgcggctgcg ctcggccgca ccggccgggg cagacgacgg
     3361 cgcgcgcgcc agcaccgagt tcgcctatgt gctcagcagc gacggcttgg cgtcgcagcg
     3421 ccagggcctg tgcgcgcctt cgctgctgcc caaggccgac accgtggtcg ccgtgctggc
     3481 cgagaacgac gtcagctggc accgcgtgaa cctgcccagg gcgccagcca atcgcctgcg
     3541 cgcggccctg tccggcctgc tcgaagaggc cttgctcgat gagaccgagc acgtccatct
     3601 ggcgctggcg ccgcagccca aggccggcga gccgacctgg atcgcggctt gcgatcaccg
     3661 ctggctggcc accgagctcg cgacgctgga gcgcgccggc gtgcgcgtgg accgcgtcgc
     3721 cccctcggcc tggcccgacg agccatccag cggccatttc cacgagttgt cggccgaggc
     3781 cggcgagcag ctgaacgtcg cgctgacctg gtcgcacggc aacggcgtcg cgacttggcc
     3841 gctgcagggc ggcctcgccc acgccctgct gccccagccc ctgcccgaga cggccggctt
     3901 caccgccacg cccgccgtcg cagcgccagc cgaacgatgg ctgggcgcta cggtggccgt
     3961 cgtttcgcct ggcgagcggg cactggcggc ggcccgctcg ctgtggaacc tgcgccagtt
     4021 cggccttgcg ccccgccatc gcggcgccga cttgctgcgc gcgcgctggc gccagttcct
     4081 cagtccggcc tggcgcccgg cgcgcgtcgg cctgctggtg ctggtgctga tgcagatcgt
     4141 cgggctcaat ctctgggcct ggcagcagaa ggcccagatc gcttcgcgcc gcgcagcgat
     4201 gaatgcgctg ctcaccacca cctatcccca ggtgcgcacc gtctatgcgg cgcccgagca
     4261 gatgcagaag gagacggagc tgctgcgcac cgctgccggc cagcccgg
//
LOCUS       DASUCY010000175         3366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_855254, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000175 DASUCY010000000
VERSION     DASUCY010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855254"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..1694)
                     /locus_tag="VFV25_03405"
     CDS             complement(105..1694)
                     /locus_tag="VFV25_03405"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEY1392391.1"
                     /translation="MRPSSRALLRACLAATLLLGLLAGPSQAGAQKMRGTALTEQRIA
                     LVIGNAAYRNDPLDNPINDARLIAGNLKLAGFSVSLHENLDRNGLVEALREFGIHLDE
                     NTVAVVYYAGHGLQLRDHNYLIPVDAEIRSEDEIPIAGVDLSFILGRMSHARSRINIV
                     ILDACRNNPFAGKTVASQGLAQMDAPVGTLLAFATAPGKLAADSGGGANSLYASYLAK
                     HLLTPGLPVEQVFKRVREGVVRDTHEQQVPWESSSLQGEFAFMPGVALAALENAAEQE
                     AAGELAFWNSIQGSMRADEYRAYLRQYPSGRFVSLAQARLAAIAVQALAPAASTPVAQ
                     APVLPALTGRPELLPHAGDSWRYRVQDQFRLGDLYLTARVGAVTADGVAETWTTTSDA
                     QVRSIVAPVEPGFHALPSWTLAPPEFSPYLLALGPLRAGQRLDDQQRRIEQVTVPLHV
                     RVEGEEEVVVAAGRFRTVKLVLRGQAQSRSAGRAGAVSTEQVVWYAPEVKRSVKTTVS
                     TYVGGSLREATTFELMEFKLN"
     gene            complement(1727..2419)
                     /gene="tagF"
                     /locus_tag="VFV25_03410"
     CDS             complement(1727..2419)
                     /gene="tagF"
                     /locus_tag="VFV25_03410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagF"
                     /protein_id="HEY1392392.1"
                     /translation="MPEAALNTSPGWYGKLPNRGDFASRRLPDDFISGWDDWLQQGLA
                     CAREDLGEEAWLARYLVAPVLRFWLAPGVLGAVAWTGLLMPSVDRVGRHFPLTVAVPA
                     GLSAGSLAAVLAAADWFSALDGAARQVLDVEFTVEDFEARLATIAVPDFDAAGDAGSP
                     GPQQLASALLQPFMPRQQQAGGPGLPGGPEASPEVPQPCSLWWREGASDAAQFACYPA
                     LPPAASFAGLLC"
     gene            complement(2401..>3366)
                     /locus_tag="VFV25_03415"
     CDS             complement(2401..>3366)
                     /locus_tag="VFV25_03415"
                     /inference="COORDINATES: protein motif:HMM:NF018454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion IcmF C-terminal
                     domain-containing protein"
                     /protein_id="HEY1392393.1"
                     /translation="AVIDPRFEALKRYVAGSPGPLDDALALLGRLATHLIAVDDALTR
                     KLMPPASDASRELASRAQLAPEPLQAMLGQLAQTSAAQELAALREPLARRMASELTPA
                     CLRALAGRYPLQRTAREEVSRAEFSRMFASGGVIDGFFQRYLAPYADTTAHPWAYRAV
                     DGSRVEPSESLPQFQRAQRLREAYFHDNGRTLGARLEFRLLELDAGIGQFTLDVDGQA
                     LHFGRESKLAQAVYWPAAGGTGRVKLQVVPMAGGSGSNYVFEGPWALFRLFDRVRIDP
                     GPSADRMQLLFDVEGRKARFEVRSMTSLNPLLRGELEQFQCPKRP"
BASE COUNT          578 a         1178 c         1128 g          482 t
ORIGIN      
        1 acggtggccc agcagactgc atagccatcc gctcaatcgg ttgcacgctg tcgcaaaccg
       61 gttcaaatac ttttcgcgac ctcgacgggc ttgccaccgt tcactcagtt gagcttgaac
      121 tccatcagtt cgaaggtcgt cgcttcacgc agcgagccgc cgacgtaggt ggacaccgtg
      181 gttttgacgc tgcgcttgac ctccggcgcg taccagacca cctgctcggt tgacaccgca
      241 cccgcccgcc ctgcactgcg cgactgcgcc tggccgcgca gcaccagctt gacggtgcga
      301 aagcgccccg ccgccaccac aacctcctcc tcgccctcga ccctgacatg cagcggcacc
      361 gtcacctgct cgatgcgccg ctgctggtcg tcgagccgct ggccggcacg cagcgggccc
      421 aaagcaagca ggtagggcga gaactcgggc ggcgcaagcg tccagctcgg caaggcatgg
      481 aagccgggct cgaccggcgc gacgatgctg cgcacctggg cgtccgaagt cgtggtccag
      541 gtttcggcca cgccgtccgc ggtgacggcg ccgaccctcg cggtcaggta caggtcgccg
      601 aggcggaact gatcctgcac gcggtagcgc caggaatcgc ccgcatgcgg cagcaactcg
      661 gggcggccgg tcaaagcggg cagcaccggc gcttgcgcga ctggagtcga cgccgctgga
      721 gcaagcgcct gcacggcaat cgccgcgagc cgggcctggg ccagcgaaac gaagcgccca
      781 ctaggatact ggcgcagata ggcacggtat tcgtcagcgc gcatcgaacc ctggatactg
      841 ttccagaacg ccagctcgcc ggccgcctcc tgctcggcag cgttttcgag cgccgccagc
      901 gcgacgcccg gcatgaaggc gaactcgccc tgcagcgagg aactctccca gggcacctgc
      961 tgctcatgcg tgtcgcgcac cacgccctcg cgcacgcgct tgaacacctg ctcgacaggc
     1021 agccccggcg tcagcagatg cttggcgagg taactggcat aaaggctgtt ggcgccacca
     1081 ccgctgtccg cggccagctt gcctggcgcg gtggcgaagg cgagcaaggt gcccaccggc
     1141 gcgtccattt gcgccagccc ctggctcgcg accgtcttgc ccgcaaacgg gttgttgcgg
     1201 caggcgtcga ggatcacgat gttgatgcgg ctgcgcgcat gcgacatgcg gccgaggatg
     1261 aagctcagat ccaccccggc gatcggtatc tcgtcttcgc tgcggatctc ggcatcgacc
     1321 ggaatcaggt agttgtggtc gcgcagctgc aggccatggc cggcgtagta gacgacggca
     1381 acggtgttct cgtcgaggtg aatgccgaat tcgcgcagtg cctccaccag gccgttgcgg
     1441 tcgaggttct cgtgcagcga gacgctaaag ccggcgagct tcaggttgcc tgcgatcagg
     1501 cgagcgtcat tgatggggtt gtccagcgga tcgttgcggt aagcggcatt gccgatcacc
     1561 agcgcaatgc gctgctcggt caacgcggtg ccgcgcatct tttgcgcccc cgcctgcgag
     1621 ggcccagcca acagccccag cagcagtgtc gcggccaggc aggcgcgcag caaggctcgc
     1681 gaagacggcc tcatgatggg cctcatccta ggacgaacag caggcttcag cacagcagcc
     1741 ccgcgaagga cgcggctggc ggcaacgccg gatagcaggc gaactgcgcc gcatcgctgg
     1801 caccttcgcg ccaccagaga ctgcagggct gcgggacctc gggtgacgct tcaggaccac
     1861 caggcaatcc cggcccgccg gcctgctgct ggcgaggcat gaagggctgc agcagcgcag
     1921 atgccagctg ctgaggcccg ggagatccag cgtcgcccgc cgcatcgaag tcgggcaccg
     1981 cgatcgtcgc gagcctggcc tcaaagtctt cgaccgtgaa ttcgacatcc agcacctggc
     2041 gtgccgcgcc gtccagggca ctgaaccaat ccgccgctgc aagcacggcc gctaggctac
     2101 ctgcggaaag tccggcgggc acggcgaccg tgagcgggaa gtggcgcccg acgcggtcga
     2161 cgctgggcat cagcagcccg gtccaggcga ccgcgcccag cacgccaggc gccagccaga
     2221 agcgcaggac gggcgccacc agatagcgcg ccagccaggc ttcctcgccc agatcctcgc
     2281 gcgcgcatgc caggccctgc tgcagccagt cgtcccagcc gctgatgaag tcgtcgggca
     2341 accggcgcga ggcgaaatca ccgcggttcg gcagcttgcc gtaccagccg ggtgaggtgt
     2401 tcagggccgc ttcgggcatt ggaactgctc cagttcgccg cgcagcagcg gattgagcga
     2461 cgtcatgctg cgcacctcga agcgggcctt gcgtccttcg acatcgaaca gcagctgcat
     2521 gcggtcggcg ctcggcccgg ggtcgatgcg cacgcgatcg aacaggcgga acaaagccca
     2581 ggggccctcg aacacgtaat tggagccgct gccacccgcc ataggcacca cctggagctt
     2641 gacgcgcccg gtgccgccag ctgcgggcca gtacacggcc tgggccagct tcgattcgcg
     2701 tccgaaatgc agcgcctggc cgtcgacgtc gagcgtgaac tgtccgatcc ccgcgtccag
     2761 ctcgagcaag cggaattcca gccgcgcgcc cagagtgcgc ccgttgtcgt ggaagtaggc
     2821 ctcgcgcagc cgctgcgccc gctggaactg cggcagggac tcgctgggtt ccacccggga
     2881 gccatccacg gcgcgatagg cccaggggtg ggcggtcgtg tcggcataag gcgcgaggta
     2941 gcgctggaag aagccatcga tcacgccgcc ggaggcgaac atccggctga actccgcgcg
     3001 agagacctct tcccgagcgg tgcgctgcag cgggtagcgc cccgccaacg cccgcaggca
     3061 cgccggcgtc agctcgctgg ccatgcgccg cgccaggggc tcgcgcaggg cagcaagctc
     3121 ctgggcagcg ctggtttgcg ccaactggcc cagcatggcc tgcaagggct cgggcgccag
     3181 ctgcgcccga gaggccaatt cgcggctcgc atcgctggca ggcggcatga gcttgcgcgt
     3241 cagggcgtca tccacggcga tcagatgcgt ggccaggcgg ccgagcagcg ccaaggcatc
     3301 gtcaagcggc cccggcgaac ccgccacata acgcttcaag gcctcgaatc tcgggtcgat
     3361 caccgc
//
LOCUS       DASUCY010000176         2692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1031924, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000176 DASUCY010000000
VERSION     DASUCY010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031924"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2692
                     /locus_tag="VFV25_03420"
     CDS             <1..>2692
                     /locus_tag="VFV25_03420"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEY1392394.1"
                     /translation="SATGHWDGPTWRLDAQLRDLAPATLDQRAPAIVLSGPLALNGRG
                     GLPPFGTGSSAGPVEVGLSGRLQGRLLGAPGQGPAGSLALLLDASLSPQRISVRQLEA
                     KAAGASAKLKGEAERVGASAAQPGAWQLRGTLALSDFDPRPWWAAARPQAGDAKPAAA
                     KADAKAVAPPPPPRINADARFDLRIPEEKAAGRKPRSLVQQLAAWRGQARAQLRDSQF
                     GGVPLAGELTLESHAARQHLLGHARLEAGGNQLTLDGEIDATAAASDRWKLQLQAPKL
                     DSLAPLLRLLRPTSPPQTSAGSLSAQAEAEGRWPSLHSRGALQAQGLSLLSWSAASAR
                     ANWDFGTTPEAAVKLQAEISQVALGQRKLDLLRAQLSGTGRSHQASLRIEAPPLRPIN
                     GASQGNGSPLDALAEQRLLALATASGGWSLDERSASPSWQGRVQQLELRALAPGAAPA
                     GNPGTAWMHAEPFELSWAGGPDSQRLSISPTRLSVSDAALKLSRLEWSQRAGAAPRFD
                     LQAELEPLKLAPLLARAQPDFGWAGDLTVDGHLALHSDAEGGFRADALLERSAGDLEV
                     VDPTQLGAKPLRLGLSEARFALGANNGLWQFSQRLTGANLGAINGEQTVSTRRELSWP
                     EPAAPLQGQLDIRVANLATWGAWVPPGWRLSGQLATQAIVGGRFGAPEYTGHLSGSHI
                     GVRNVLAGVNLSEGEVEIALQGDTARIERFNVRGGQGQARLEGEASLGAAPHARLQLI
                     ADRFAALSRVDRRLSLSGQAQLELQSKALKLDGRLVADSGLVDISRGGAPSLADDVTV
                     VRRGSGDAAAALSEPAKTAPRMSNVALNLKLDLGPDFRLRGHGLDTLLRGELAVTTPG
                     GLMNVKGSIRTEEGSYAAYAQKLDIDRGIISFDGPVENPRL"
BASE COUNT          412 a          993 c          964 g          323 t
ORIGIN      
        1 cagcgccact ggccactggg acggccccac ttggcgactg gacgcgcagc tgcgggatct
       61 cgcgcccgca acgctggatc agcgcgcgcc ggccatcgtg ctgagcgggc cgctcgcgct
      121 gaacggccgg ggcgggctgc cgcccttcgg cacggggagc tcggccggcc cggtcgaagt
      181 cggcctgagc ggccgcttgc agggccgcct gctcggcgca ccaggacaag gcccggccgg
      241 gtccttggcc ctgctgctcg atgccagcct gagtccgcag cgcatcagcg tgcggcaact
      301 ggaagcgaag gctgccggag ccagcgccaa gctcaaaggc gaggccgagc gcgtcggcgc
      361 ctccgccgcc caaccgggcg cctggcagct gcgcggcacg ctggccttgt cagacttcga
      421 tcctcgcccc tggtgggctg ctgcaagacc gcaggccggc gatgcaaagc cagccgctgc
      481 aaaggccgac gccaaggcag tcgcgccacc accgccgccg cgcatcaatg cggacgcgcg
      541 attcgacctg cgcatccccg aagaaaaggc agccggacgc aagccgcgca gcctggtgca
      601 gcagctcgcg gcttggcgcg gccaggccag ggcgcagctg cgcgacagcc agttcggtgg
      661 cgtaccgctg gccggcgagc tcacgctgga aagccacgcg gcacggcagc acctgctcgg
      721 ccacgccagg ctggaggcgg gcggcaatca gctgactctg gacggggaaa tcgacgccac
      781 cgccgctgcc agcgaccgct ggaagctgca gttgcaggcc cccaagctcg acagccttgc
      841 cccgctattg cgcctgctgc ggcctacctc gccaccccag acttccgccg ggtccctgag
      901 cgcccaggcc gaggccgagg gccgctggcc ctcgctgcac agccgcggcg cgctgcaggc
      961 ccagggcctg agcctgctgt cctggagcgc agccagcgca cgggccaact gggacttcgg
     1021 cacgacgccc gaggccgccg tcaagctgca ggcggaaatc agccaagtcg cgctcgggca
     1081 gcgcaagctt gatctgctgc gcgcccagct gagcggcact ggccgctcgc atcaggccag
     1141 cttgcgcatc gaagcgccgc cactgcgacc aatcaacggc gcgagccaag gcaacggatc
     1201 gcccctggac gccctcgccg agcagcggct gctcgcgctc gcaacggcca gcggcggctg
     1261 gtcgctcgac gaacgctccg caagcccaag ctggcagggg cgcgtacagc agctggagct
     1321 gcgcgcattg gcgcccggtg cggcgccggc cggcaatcca ggcacggcct ggatgcatgc
     1381 cgaacccttc gagctgagct gggccggcgg cccggacagc cagcgcctga gcatctcgcc
     1441 gacgcggctg agcgtctcgg acgctgcgct caagctgagc cgcctagagt ggtcgcagcg
     1501 cgccggcgcg gcaccccgct tcgatctgca ggccgaactg gagccgctga agctggcgcc
     1561 gctgctggcc cgcgcgcagc ccgacttcgg ctgggccggc gatctgacgg tagacggcca
     1621 tctcgcgctg catagcgatg ccgaaggcgg tttccgcgcc gacgccttgc tggagcgcag
     1681 cgccggcgac ctcgaggtgg tcgaccccac gcagctcggc gccaagccgc tgcgcctggg
     1741 actgagcgaa gcccggttcg ccctcggcgc gaacaacggg ctgtggcagt tctcccagcg
     1801 gctgacaggg gccaacctcg gggctatcaa cggcgagcag accgtgagca cgcgccgcga
     1861 actgagctgg cccgagcctg cggcgccgct gcagggccag ctcgacatcc gcgtcgccaa
     1921 tctcgccacc tggggcgcct gggtcccgcc gggctggcga ctttccgggc agctggccac
     1981 gcaggccatc gtgggcgggc gcttcggggc gcccgaatac accggccacc tgagcggcag
     2041 ccacatcggc gtgcgcaacg tgctggccgg cgtgaacctg agcgaaggcg aagtcgagat
     2101 cgcgttgcag ggcgacacgg cgcgcatcga gcgcttcaac gtgcgcggcg gccagggcca
     2161 ggcgcggctg gagggcgagg ccagcctggg cgccgcgccg catgcgcggc tccagctcat
     2221 tgctgatcgc ttcgcggcgc tgagccgcgt cgatcgccgg ctgtcgctca gcggccaggc
     2281 ccagctcgag ctgcagagca aggcgctcaa gctcgacggg cggctggtgg ccgacagcgg
     2341 cctcgtcgac atctcgcgcg gcggcgcgcc cagcctcgca gacgacgtga cggtcgtgcg
     2401 ccggggcagc ggcgatgccg cggccgccct ctccgagccg gccaagacgg cgccgcgcat
     2461 gagcaatgtc gcgctgaacc tgaagctgga cctgggaccg gacttccgcc tgcgcggaca
     2521 tggcctggac accctgctgc gcggcgagct tgccgtcacg acgcccggcg ggctgatgaa
     2581 cgtgaagggc agcatacgca ccgaggaggg cagctatgcg gcctacgccc agaagctgga
     2641 catcgatcga ggcatcatca gcttcgacgg ccccgtcgag aacccgcggc tg
//
LOCUS       DASUCY010000177         2768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_100261, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000177 DASUCY010000000
VERSION     DASUCY010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100261"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..1032
                     /locus_tag="VFV25_03425"
     CDS             223..1032
                     /locus_tag="VFV25_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392395.1"
                     /translation="MATQPLHPEKRIHPLHGDPIRIVPLHLPELVSTPTADALAAGPA
                     AAPQLTYRNGPLLANVEVYTIFWGPAWRHAAQTTLMNQLNQFFDFVLSSPLIDQLAEY
                     DVPNYKIGHGKRTGTINILTPALKHSVSDTAVQHMLQFEIANNPAVPHPGPNTLYFVY
                     VPPGVRVIQGGSASCQAFCGYHNDIAGQIFYAVMPYPGCGGCTGGLSVLDALTSTSSH
                     ELCEAITDAIPGQGWYDDTNGEIGDICAWKTKKLGPYTVQLEWSNAANGCV"
     gene            complement(1057..2361)
                     /locus_tag="VFV25_03430"
     CDS             complement(1057..2361)
                     /locus_tag="VFV25_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392396.1"
                     /translation="MKYTVLVSAALLAGCTTYVTAPPPPRAYEPPPVSYQPPPPAVVA
                     SVYVEPPIEQPAPIAAPWAPPPMLVEVPPPMPFEGAVWIGGYWLWNGTWVWGHGRWAE
                     PPQPTYHWVQPYYENRSGAVIFITGHWAAAGVVFVPPPPTLSISIAVASAGVVPGPRP
                     IGPNGVFVPPPPGSRPGIIVPAPIGVAPAVVTSAPPVVNVGMRINASVNNSNNTNIVN
                     NNINRTTNITNVTNVTNVTNVTIVAPPSATAAGRPVSLAVPTQQHLAAALPAVVRAPA
                     PVVPPPPPRTAAQPMPQQWQGQRPQAAEAMPQRPAAPARAQEPAYAPSRPGDAAWAAR
                     PPAAAPSPAPRNAYPAADPRMPAQHPAQEEGMQRPAGPRGEWAEQPRPQPPRGPAPEA
                     ARQNLERQAAEQRKAAEKKAQQQARERAGDKEREGKRDEQQR"
BASE COUNT          421 a          910 c          973 g          464 t
ORIGIN      
        1 cacgaatacc tccaaggtcg acgactcgtg aatggatcgg acaacgccgc cacgcggcgg
       61 ccgaggcagg gattgtgtgc cgacacggcg gtgcgggcaa ccggccgctg tgccaggacg
      121 taagtcatcc tcggcgggct acccaaaccc actcggccga cctaggcttg tccctccatt
      181 cggcccgcaa gccgcagccg ttcgccaaag gaggacaaga tcatggccac ccagccgctt
      241 cacccggaaa agaggatcca cccgctgcac ggcgacccga tcaggatcgt gccattgcat
      301 ctgcccgagc tggtgtcaac gcccacggcc gatgcgctcg cagccgggcc ggccgccgct
      361 ccccagctga cctaccggaa cgggccgctg ctcgcgaatg ttgaggtcta caccattttc
      421 tggggcccgg cctggcgtca cgcggcgcag acgacgctga tgaaccagct caaccagttc
      481 ttcgatttcg tgctcagcag cccgctgatc gatcagctgg ccgagtacga cgtgcccaac
      541 tacaagatcg gccacggcaa gcgcaccggc accatcaaca tcctcacgcc ggccctcaag
      601 cattcggttt ccgatacggc cgtgcagcac atgttgcagt tcgagatcgc caacaacccg
      661 gccgtgcccc atcctgggcc gaacaccctg tacttcgtct atgtgccgcc gggcgtgcgc
      721 gtgatccagg gcggctcggc gtcctgccag gccttctgcg gctatcacaa cgacatcgcc
      781 ggccagatct tctacgccgt gatgccctat ccgggctgcg gcggctgcac cggcggcctg
      841 tcggtgctgg acgcgctgac ctcgaccagc tctcacgagc tgtgcgaggc gatcaccgat
      901 gcgattcccg gccagggctg gtacgacgac accaacggcg agatcggcga catctgcgcc
      961 tggaagacca agaagcttgg gccatacaca gtgcagctgg aatggtcgaa cgcagccaat
     1021 ggctgcgttt gacctggatt gcgcgcctcg gcgccgtcag cgctgctgct cgtcacgctt
     1081 gccttcgcgc tccttgtcgc cggcgcgttc acgtgcctgt tgctgggcct tcttctcggc
     1141 ggctttgcgc tgttcggcag cttgcctctc gaggttctgc ctggcggcct cgggcgcggg
     1201 gccccgcggc ggctgaggac gcggctgctc tgcccattcg ccccgcggtc ccgctggacg
     1261 ctgcatgcct tcctcttgcg ccggatgctg cgcgggcatg cggggatcag ccgcgggata
     1321 cgcgttgcga ggcgcggggc tgggcgctgc cgcaggcggg cgagcggccc aggcggcgtc
     1381 gcccggcctg ctgggcgcat aggccggctc ctgggcgcgc gccggcgcag cagggcgctg
     1441 cggcatagct tcggcggcct gggggcgctg gccttgccac tgctgcggca tgggctgagc
     1501 ggccgtgcgc ggcggaggcg gcggcactac tggtgccggc gccctgacaa ccgcaggcag
     1561 ggccgcggcg aggtgctgtt gcgtcggcac ggcgaggctc accgggcggc cggccgcagt
     1621 cgcactgggc ggggccacga tggtcacatt ggtcacgttc gtgacattgg tgacattcgt
     1681 gatgttggtc gtccggttga tgttgttgtt gacgatgttc gtgttgttgc tgttgttaac
     1741 gctggcattg atgcgcatgc cgacgttgac caccggcggc gcgctggtca ccacggccgg
     1801 cgccacgccg atcggggccg gcacgatgat gccggggcgc gagcccggcg ggggcggcac
     1861 gaacacgccg ttgggcccga tggggcgcgg gcccggcacg acgccggcag acgccacggc
     1921 gatcgagatg ctgagcgtgg gcggcggcgg cacgaagacc acgcccgccg cggcccagtg
     1981 gccggtgatg aagatcaccg cgccactgcg gttctcgtaa tagggctgga cccagtggta
     2041 ggtgggctgg ggcggttcgg cccagcggcc atggccccag acccaggtgc cgttccataa
     2101 ccagtagccg ccgatccaga cggcgccttc gaaaggcatg ggaggcggca cctcgaccag
     2161 catgggcggc ggcgcccacg gtgcggcgat gggcgcgggc tgctcgatgg gtggctcgac
     2221 gtagacgctg gcaacgacag ccggcggcgg aggctggtag ctcacgggcg gcggctcgta
     2281 ggcgcgcggc gggggcgggg cggtgacata agtggtgcag ccagccagca gcgcagcgct
     2341 gacaaggacg gtgtatttca tggtgtgtcg cctcgggaac ttgtccgctc gggccggcgc
     2401 ctcggcggct tgatttgttg cgggtagatc gagtgaacgc tcagctaacg gctcgcgtcg
     2461 atcccgtgtt gactgatttg aacccgcctt gcctgactcg ccgttgacgc aaggagggtc
     2521 ccggcagcat ggcgggcggg cgcgatgcag cctgcggtta cgcgctcatc cctattttgt
     2581 gtgatgcaca ttcgccgttc gccgcctaat atcaattgga tttgacacat atctgggtgg
     2641 gccttggcgg cgtttgtgcc gcaaagcagc cgcacccaaa gggggaaaga tgctagcgag
     2701 cgagcgaaca cttgccgatg ccgcacagaa ggtcccgaca gccgatgccg cgcgtgcatc
     2761 gctgaagg
//
LOCUS       DASUCY010000178         4556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1465646, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000178 DASUCY010000000
VERSION     DASUCY010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4556
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1465646"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VFV25_03435"
     CDS             <1..312
                     /locus_tag="VFV25_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY1392397.1"
                     /translation="LSTSRSRRESTEAIKTRGNLTTNDGEIAVNWALDGHGILMRAEW
                     DIERHLRSGRLVQVLPQYFTPDADIYAVYPQRHQLSARVRAFVDFLTLSFQQQLGGSR
                     E"
     gene            complement(334..1245)
                     /locus_tag="VFV25_03440"
     CDS             complement(334..1245)
                     /locus_tag="VFV25_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEY1392398.1"
                     /translation="MTTTSKLAVETTPQGPRLSRIIAGAWRMADWRLTPQERLSWIKQ
                     CVELGVTSFDHADIYGDYAVEALFGEALALDRSVAQQIQIVTKCGIKLMSSKRPQHAI
                     KSYDTSREHIVRSVDHSLQALGVERLDVLMIHRPDALMDPAAVAETFRDLRRAGKVAH
                     FGVSNHSASQFALLHRQLPLATHQIEFSPLQMKALDDGTLDQCVEQNIRPMIWSPLGG
                     GRLFTGEDAQAQRVRAELVRLGSLYGVPPETIAYAWLLRHPARPLPISGSRRIEALRA
                     AVRALEVELSAEDWYAVWQAGAGREVP"
     gene            complement(1242..3683)
                     /locus_tag="VFV25_03445"
     CDS             complement(1242..3683)
                     /locus_tag="VFV25_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020700939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HEY1392399.1"
                     /translation="MLVGLVLASASAAEAAPMPPGGYSVSGNSIVLTDAQGTRLRITP
                     YGSFAVRLQAAREGEAFFPDDHYEMVESHRHGGRFKVQATDAEIRLTQPGGLRLHIDR
                     KTLAVAYFAADGDAALLQETGGVRWENGSITRGFVPDEREHFTALGHGFFGRADAIDL
                     KGQRLGRNYGTQQIDQAPLIVPFYISSRGYGVFLNSSFRNFFDFLQDGRYEFGIEPGA
                     DAGRMDYFVIAGPQPADVLRHYVELTGKPRLPPKAIFGLALSDKSHDHDSPTPSDEAW
                     WKRQVLAHRAAGLPIDHLVNDNRWRAGGGKRCESRMEWDTGRFPDPAEYARWLKADGL
                     VTTIDINRCVSQFSEGWKPAFNIPQAPGVDFASSAPDLTNAEFRAWFWNIFYRKALDP
                     KLGYPGDALWIDEFDEMGAAPDSMKLASGQSFGEMRNYWFFLIAKALVQQGWDRSGLD
                     RRPYVWVRGMTAGAQRYATLWSGDIKPNDDEMKQQVRGMQLAGLSGFPFWGHDAGGFY
                     DWQAKRGPDASLHERWSMGWGSFSPIWKPHGMGPSRWPLDRTPPEQAAAARFARARYE
                     LMPYIYTAAHEAARTGLPMARALLLAYPQVEKAWRNDLEYLWGPDLLVAPNTDPEHDQ
                     EVWLPPGRWFDYWHPKQAIAGDSVVRIARQTEDLPVFVKAGAVLVKQPYALSTAFADK
                     RKLMLDVYAGADGETTLVEDDDVTEDYRLHGRQMSTRIRYDDARKRLHVSGALGHYRG
                     APKQRAWEVTLHGPDLGSCYLVNGRRTEATQEDDGSAMRIVIASADIRKDTIIQACGN
                     RAAARVAMQHSEDHK"
     gene            3889..>4556
                     /locus_tag="VFV25_03450"
     CDS             3889..>4556
                     /locus_tag="VFV25_03450"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY1392400.1"
                     /translation="MAITTEKPTSEVPAIRRVRDLPGPRGLPLLGNTLQIKRDRIHQQ
                     AERWARQFGEAYRFRITSREIVVLSNPETVAAILRDRPDGFQRTERLNETARTFGFDG
                     LFSSNGEAWKRQRPMVLSGLDPTHIKAFFPTLAKVTQRLARRWRQAALAGQTIDLQAD
                     LMRYTVDVTAGLAFGTDINTIESSEEVIQTHLDKILPALSGRLFAPVQYWRYFKLPAD
                     YALGR"
BASE COUNT          703 a         1523 c         1558 g          772 t
ORIGIN      
        1 ctctcgacga gccgctcgcg tcgcgagagc accgaggcca tcaagacccg cggcaatctg
       61 accacgaacg acggagaaat cgccgtcaac tgggccctag acggccacgg catcctgatg
      121 cgcgccgaat gggacattga gcgccacctg cgcagcggca ggctggtgca agtgctgccg
      181 cagtatttca cgccggatgc cgacatctac gccgtctatc cgcagcgcca ccagctgtcg
      241 gcgcgagtgc gcgccttcgt tgacttcctt acgctgtcgt ttcagcagca gttgggagga
      301 agccgggagt agcgtgaagc gcgcaggctg gcatcagggc acttcgcgcc ccgcgccggc
      361 ctgccagacg gcataccagt cctcggccga gagctcgact tccagtgcgc ggacggcggc
      421 gcgcagtgct tcgatgcgcc gcgagcccga gatgggcagc ggccgggcgg ggtgacgcag
      481 cagccaggca taggcgatgg tctcgggcgg cacgccataa aggctgccca agcgaacgag
      541 ctcggcccgc acgcgctgcg cctgcgcatc ttcaccggtg aagagccgcc cgccgcccag
      601 cggcgaccag atcatgggcc tgatgttctg ctccacgcat tgatcgagcg tgccgtcgtc
      661 cagcgccttc atctgcagcg gcgagaactc gatctgatga gtggccagcg gcagctggcg
      721 gtggagcagc gcgaactgcg atgcggaatg gttcgagacg ccgaagtggg ccaccttgcc
      781 ggccctgcgc agatcgcgga aggtttccgc cacggccgcc gggtccatca gcgcatcggg
      841 ccggtggatc atgaggacat cgagccgctc cacgcccaac gcctggagcg agtggtcgac
      901 cgagcggacg atgtgctccc tggacgtgtc atagctcttg atcgcatgct gcggccgctt
      961 ggaggacatc agcttgatgc cgcatttggt gacgatctgg atctgctggg cgaccgatcg
     1021 gtccagggcc agggcctcgc cgaacaaggc ctcgactgca tagtcgccat agatgtcggc
     1081 gtgatcgaag ctggtcacgc cgagctcgac gcattgcttg atccagctca gcctctcctg
     1141 gggcgtgagg cgccagtcgg ccatgcgcca ggcgccggca atgatccggg agagtcgcgg
     1201 cccttgcggg gtcgtttcga cggccagttt ggaggtggtg gtcatttgtg atcctcgctg
     1261 tgctgcatgg cgactcgcgc tgctgcacga ttaccgcacg cctggatgat ggtgtctttg
     1321 cggatgtcgg cgctggcaat gactatcctc atcgcgctgc cgtcatcttc ttgcgtggcc
     1381 tcggtacggc gcccgttcac caagtagcag ctgccgagat ctggcccgtg cagcgtcacc
     1441 tcccaggccc gctgtttcgg cgcgccgcga tagtggccga gcgcgccgga aacgtgcagg
     1501 cgcttcctcg catcgtcata gcggatgcgc gtggacatct ggcggccgtg caggcggtag
     1561 tcctcggtca cgtcgtcgtc ctcgaccagc gtggtttcgc cgtcggcgcc tgcgtagaca
     1621 tcgagcatga gtttgcgctt gtcggcgaag gccgtgctca gtgcataggg ctgcttgacc
     1681 agcacggcgc cggccttgac gaagacgggg aggtcctcgg tctgccgcgc aatgcgcacg
     1741 acgctgtcgc cggctatggc ctgcttggga tgccagtagt cgaaccatcg ccccggcggc
     1801 agccagacct cctggtcatg ctcgggatcg gtgttcggcg ccaccagcag atccggcccc
     1861 cagaggtatt ccaggtcgtt gcgccaggcc ttctccacct gcggataggc gagcagcagt
     1921 gccctggcca tcggcaggcc ggtgcgcgcg gcctcgtgcg ccgcggtata gatgtagggc
     1981 atcagctcgt agcgggcgcg ggcgaagcgg gcggctgcgg cctgctcggg cggcgtgcga
     2041 tccagcggcc agcgcgacgg ccccatgcca tgcggcttcc agatcggcga gaagctgccc
     2101 cagcccatgg accagcgctc gtggaggctg gcatcggggc cgcgcttggc ctgccagtcg
     2161 tagaagccgc cggcatcgtg gccccagaac gggaagccgg acaagcccgc cagctgcatg
     2221 ccgcgcacct gctgcttcat ctcgtcgtcg ttgggcttga tgtcgccgct ccacagggtg
     2281 gcatagcgct gcgcgcccgc tgtcatgccg cgcacccaga catagggccg ccggtccagc
     2341 ccggagcggt cccagccttg ctgcacgagg gccttggcga tcaggaagaa ccagtaattg
     2401 cgcatctcgc cgaagctctg gccgctggcc agcttcatcg agtccggtgc cgcgcccatc
     2461 tcgtcgaact cgtcgatcca tagcgcgtcg cccgggtagc ccagcttcgg gtccagggcc
     2521 ttgcggtaga agatgttcca gaaccaggcg cggaattccg cgttggtcag gtcgggcgcg
     2581 ctgctggcga agtccacgcc cggggcctgc gggatgttga acgcgggctt ccagccctcg
     2641 ctgaactgcg agacgcagcg gttgatgtcg atcgtggtca cgaggccgtc ggccttgagc
     2701 cagcgcgcgt actcggcggg gtccgggaag cggcccgtgt cccattccat gcgcgactcg
     2761 cagcgcttgc cgccgccggc gcgccagcgg ttgtcgttga ccaggtggtc tatcggcagc
     2821 ccggcagcgc gatgcgcgag gacttgccgc ttccaccaag cctcgtcgga cggcgttggc
     2881 gagtcgtggt cgtggctttt gtcggacagg gccagcccga agatggcctt gggcgggagg
     2941 cgcggcttgc cggtcagctc gacatagtgg cgcagcacgt cggccggctg cggcccggcg
     3001 atcacgaagt agtccatgcg gccggcgtcg gcgcccggct cgatgccgaa ctcgtagcgg
     3061 ccgtcctgca ggaagtcgaa gaagttcctg aagctgctgt tgaggaagac gccatagccc
     3121 ctgctggaga tgtagaaggg cacgatcagc ggtgcctggt cgatctgctg ggtgccgtaa
     3181 ttgcggccga ggcgctggcc cttcaggtcg atagcatcgg cgcggccgaa gaagccatga
     3241 ccgagcgcgg tgaagtgctc gcgctcgtcc ggcacgaagc cgcgcgtgat gctgccgttt
     3301 tcccagcgca cgccacccgt ctcctgcagc agcgctgcgt cgccgtcggc agcgaagtag
     3361 gcgaccgcga gggtcttccg gtcgatatgc aggcgcagcc cgccgggctg ggtcaggcgg
     3421 atctcggcat ccgttgcctg gaccttgaag cgcccgccat gccggtgcga ctcgaccatc
     3481 tcgtaatggt cgtccgggaa gaaggcctcg ccctcgcgcg ccgcctgcag gcgcaccgcg
     3541 aagctgccgt aaggcgtgat cctgagccgg gtgccctggg cgtcggtcag gacgatgctg
     3601 ttgccgctga cgctgtagcc gcctggcggc atcggggctg cctcggcggc cgaggcgctc
     3661 gcgagcacga gcccgactag caatgacccg atccggaccg cccacgcaga ccggtgcttc
     3721 cttgtgctct ctcttgacga catctgaacc ctccaggcgc gcggattgtc gtgtaggcga
     3781 tacctgccgg gtacaggccg gttacgagct cctccggctg ctgcgcgatt ccccgcgtcg
     3841 ggggccgtgc cggcggcata ctgtgccgat ccgctagcga gggctctgat ggccattacc
     3901 accgaaaagc cgacgagcga ggtgcccgcg atacgccgcg tccgcgatct gccagggccg
     3961 cggggcttgc cgttgctcgg caataccttg cagatcaagc gcgatcgcat acaccagcag
     4021 gccgagcgat gggcgcggca gttcggggaa gcctatcgct tccgcatcac ctcgcgcgag
     4081 atcgtggtgc tgtccaaccc ggaaaccgtg gccgctatcc tgcgcgaccg gcccgatggc
     4141 ttccagcgga ccgagcggct caacgaaacc gcgcgcacct ttggcttcga tggcctcttc
     4201 tcgtccaacg gcgaagcctg gaagcgccag cggcccatgg tgctcagcgg gctggacccg
     4261 acccacatca aggctttctt ccccacgctg gccaaggtca cgcagcgcct cgcgcgccgc
     4321 tggcggcagg ctgcgctggc cggccagacg atcgacctgc aggccgacct gatgcgctac
     4381 accgtcgatg tcacggcggg gctggccttc ggcaccgaca tcaacacgat cgaatccagc
     4441 gaggaggtca tccagaccca tctcgacaag atcctgcccg cgctgtccgg gcgcttgttc
     4501 gcgccggtcc agtactggcg gtatttcaag ctgccggccg actacgcgct gggccg
//
LOCUS       DASUCY010000179         5008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_260864, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000179 DASUCY010000000
VERSION     DASUCY010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 5008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5008
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260864"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="VFV25_03455"
     CDS             <1..997
                     /locus_tag="VFV25_03455"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEY1392401.1"
                     /translation="ELLALNTPGEAAERGGELHRAALAANRLARTYAELVAQLLRDAQ
                     TGPESVLAIGAHGQTIRHRPGEFDGVGYTLQINAPALLAELSGIDVVADVRGRDVAAG
                     GQGAPLAPILHQAIFGRPGETTAALNIGGISNLTVIPSGASAWGYDCGPGNALMDHWC
                     AQHLGQPYDRDGAWAASGRMLQPLLEAALREPYFALPPPKSTGRDLFNPAWLERLLSA
                     LPQPPVPADVQATLCELTAQCCAAGLKQHAGTARELIVCGGGAYNGELMRRLAARLPG
                     VKVASSAEHGLPPLQVEAAAFAWLARAHLHREPGNLVAATGARGPRVLGALYPFR"
     gene            complement(1063..1428)
                     /gene="erpA"
                     /locus_tag="VFV25_03460"
     CDS             complement(1063..1428)
                     /gene="erpA"
                     /locus_tag="VFV25_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEY1392402.1"
                     /translation="MSAVAENVTTEMPAPLVFTDSAAAKVRELVEEEGNPDLKLRVFV
                     QGGGCSGFQYGFTFDEIINDDDTQMQKNGVTLLIDAMSLQYLVGAEIDYKDDLEGAQF
                     VIKNPNATSTCGCGSSFSA"
     gene            complement(1468..1860)
                     /locus_tag="VFV25_03465"
     CDS             complement(1468..1860)
                     /locus_tag="VFV25_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEY1392403.1"
                     /translation="MFGAKKQPPIHTLVGEGTVIQGELRFSAGLRIDGEVIGDVLASG
                     ERTILVISEKASVTGKVKAGHVIINGTVKGPVESSELLELQPKACIEGDVCYEALEMH
                     QGATIAGVLRPLKGGDQAAVFKLASNNA"
     gene            complement(1956..2666)
                     /locus_tag="VFV25_03470"
     CDS             complement(1956..2666)
                     /locus_tag="VFV25_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6776 family protein"
                     /protein_id="HEY1392404.1"
                     /translation="MRWKLLRRRLSISSPRMTVRSHLPWPLRWAAVALMCGFSAAIAL
                     WAFEFGKDIAGLDHRSAADIAALKRELEQLRQERDQAQAIANTADSLLKAGTAAQDKL
                     AAQVKALETQNLALRGDLGFFERLLPAGGGESLAVRALQAEVPTAGQLHFQLLVMQQG
                     KARPEFHGSYEFTLSGELQDKPWSQAMPGGARPLQFKQYQRLDGVIDYPAAAVVKLVQ
                     VKVMDHDGGVRATQSLHM"
     gene            complement(2688..3725)
                     /gene="argC"
                     /locus_tag="VFV25_03475"
     CDS             complement(2688..3725)
                     /gene="argC"
                     /locus_tag="VFV25_03475"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEY1392405.1"
                     /translation="MIKVGIVGGTGYTGVELLRLLSQHGQVELRAITSRKEAGLPVAD
                     MYPSLRGHVDLAFVTPDDAKLAECNVVFFATPHGVAMAQARELLAAGVKIIDLAADFR
                     LKDVAEFERWYGMPHGCPDLLAEAVYGLPELNREAIKKARVVGNPGCYPTSVQLGFAP
                     LLKHKLVDGAHLIANCASGVSGAGRKAEVHSLLSEASDNFKAYGVKGHRHGPEINQQL
                     RVIAGDAGVNCIFVPHLLPIIRGIHSTLYARLNADGQAADLQALFEGFYKGERFIDVL
                     PAGSAPETRSVRASNTVRIAVHQPQPDTVMVLVVEDNLTKGASGQAVQCMNLMCGLPE
                     HLGLTQVPVLP"
     gene            complement(3847..4239)
                     /gene="rpsI"
                     /locus_tag="VFV25_03480"
     CDS             complement(3847..4239)
                     /gene="rpsI"
                     /locus_tag="VFV25_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEY1392406.1"
                     /translation="MIGNWNYGTGRRKSSVARVFIKKGTGQITVNGKPVEQYFGRQTS
                     IMIVKQPLFLTNNVEAFDIKVNVHGGGESGQAGAVRHGVTRALIDYDAALKSELSRAG
                     FVTRDAREVERKKVGLHGARRRKQFSKR"
     gene            complement(4249..4677)
                     /gene="rplM"
                     /locus_tag="VFV25_03485"
     CDS             complement(4249..4677)
                     /gene="rplM"
                     /locus_tag="VFV25_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEY1392407.1"
                     /translation="MKTFSAKPADVKHEWFVIDATDKVLGRVASEVALRLRGKHKAIY
                     TPHVDTGDFIVIVNADKIRVTGNKAEQKVYYRHSGFPGGIYGTKFKDMQAKHPGRALE
                     KAVKGMLPKGPLGYAMIKKLKVYAGATHPHTAQQPKALEV"
BASE COUNT          796 a         1664 c         1647 g          901 t
ORIGIN      
        1 cgagctgctc gcgctcaaca cgcccggcga ggctgccgag cgcggcggcg agctgcaccg
       61 cgcggcgctg gcggccaacc gcctggcgcg gacttatgcc gagctggtcg cgcaactgct
      121 gcgcgacgcg cagaccggcc ccgagagcgt gctcgccatc ggcgcccatg ggcagaccat
      181 caggcatcgc cccggcgagt tcgatggcgt cggctacacg ctgcagatca acgcgccggc
      241 actgctggcc gagctgagcg gcatcgatgt cgtggccgac gttcgcggcc gcgatgtcgc
      301 ggcgggcggc cagggcgcgc cgctcgcacc catcctgcac caggccatct tcggccggcc
      361 gggtgagacg acggccgcgc tcaacatcgg cggcatcagc aatctcactg tgatcccgag
      421 cggggccagc gcctggggct atgactgcgg gccgggcaac gcgctgatgg accattggtg
      481 cgcgcagcat ctgggccagc cttatgaccg cgacggcgcc tgggccgcca gtggccgcat
      541 gctgcagccc ctgctggagg cggccttgcg cgagccctat ttcgccttgc cgccgcccaa
      601 gagcaccggg cgcgatcttt tcaacccggc ctggctggag cgcctgttga gcgccctgcc
      661 gcagccgccc gtgccggccg atgtgcaggc cacgctgtgc gaactgaccg cgcaatgctg
      721 cgcggccggg ctgaagcagc acgccggcac ggcgcgggag ttgatcgtct gcggcggcgg
      781 cgcctacaac ggcgagctga tgcgccggct ggcggcgcgg ctgccgggcg tcaaggtggc
      841 ctcatcggcc gagcacggcc tgccgcctct tcaggtcgag gccgcggcct tcgcctggct
      901 ggcccgcgcc catctgcatc gcgaacccgg caatctcgtc gcggccaccg gtgcgcgcgg
      961 gccccgggtg ctgggcgcgc tctatccctt tcgatagctt tttcgcctgc tacccaagtg
     1021 aaaaagccgc cccatgggcg gctttttgta gcgcgcgcgt cgtcaggccg agaagctgga
     1081 gccgcagccg caggtgctcg tggcgttggg gttcttgatc acgaactggg cgccttcgag
     1141 gtcgtccttg tagtcgatct cggcgccgac caggtattgc aggctcatcg cgtcgatcag
     1201 cagcgtgacg ccgttcttct gcatctgggt gtcgtcgtcg ttgatgatct cgtcgaaggt
     1261 gaagccgtac tggaagcccg agcagccgcc gccctgcacg aagacgcgca gcttcaggtc
     1321 cggattgccc tcttcctcga ccagttccct gaccttggcg gccgcactgt cggtgaagac
     1381 cagcggggcg ggcatttcgg tggtgacgtt ttctgcgact gcgctcatgg aaactcctgc
     1441 ttggcgtact gctcctgacc ggaattgtca ggcattattg gacgccagtt tgaaaactgc
     1501 ggcctgatcg ccgcccttca agggcctgag cacgccggcg atggtcgcgc cttggtgcat
     1561 ttccagcgcc tcgtagcaga catcgccctc gatgcaggcc ttgggctgca gctccagcag
     1621 ctcggaagac tccaccggcc ccttgacggt tccgttaatg atgacatgac cggccttgac
     1681 cttgccggtc acgctggctt tctcgctgat gacgaggatg gtgcgctcgc cgctggctag
     1741 cacgtcgccg atcacctcgc cgtcgatgcg caggcccgcg gaaaaccgca gctcgccctg
     1801 gatcacggtt ccttcgccca ccagggtgtg gatgggcggc tgcttcttcg caccgaacat
     1861 ggctactcct acaacaacga ccgctttttg atgactgaag gcgccgatct ttgtcggctg
     1921 cctgcccgaa tcaggcaata gagcaaaaga agtgcctaca tatgcaggct ctgcgtggcg
     1981 cgcacgccgc catcgtggtc catcaccttg acctgcacca gcttgaccac cgccgccgcg
     2041 ggatagtcga tcacgccgtc cagccgttgg tattgcttga actgcaaagg cctggcgccg
     2101 ccgggcatgg cctggctcca gggcttgtcc tgcagctcgc cgctgagcgt gaattcatag
     2161 ctgccgtgga actcgggcct ggccttgccc tgctgcatta cgagcagctg gaaatgcagc
     2221 tggcccgccg tcggcacctc ggcctgcagg gcgcgcaccg cgagcgattc gccgccgccg
     2281 gcgggcagca ggcgctcgaa gaaacccaga tcgccgcgca aggcgaggtt ctgcgtctcc
     2341 agcgccttga cctgggctgc cagcttgtcc tgcgccgccg tgccggcctt gagcaggctg
     2401 tcggcggtgt tggcaatcgc ctgggcctga tcgcgctcct ggcgcagctg ctcgagctcg
     2461 cgcttgagcg ccgcgatgtc cgcggcggag cggtgatcga gcccggcgat gtccttgccg
     2521 aattcgaagg cccacaacgc gatcgccgcc gagaagccgc acatcagcgc caccgcagcc
     2581 cagcgcaagg gccaaggcag atggctgcgc accgtcatgc gcggtgagct gatcgaaagg
     2641 cggcggcgca acagcttcca gcgcatgcgc gaagccctag cgcggcctca aggcagcacc
     2701 ggcacctggg tcagccccag gtgctcgggc aggccgcaca tcagattcat gcactgcacg
     2761 gcctggccgg acgcgccctt ggtcaggttg tcttccacca ccagcaccat cacggtgtcg
     2821 ggctggggct ggtgcacggc aatgcgcacc gtgttgctgg cgcgcacgct gcgcgtttcg
     2881 ggcgccgagc cggccggtag cacgtcgatg aagcgctcgc ccttgtagaa gccttcgaac
     2941 agcgcctgca ggtcggccgc ctgcccgtcg gcattgagcc gcgcatagag cgtggaatgg
     3001 atgccgcgga tgatcggcag caggtgcggc acgaagatgc agttcacgcc ggcatcgccc
     3061 gcgatcacgc gcagctgctg gttgatctcg gggccgtggc ggtggccctt gacgccatag
     3121 gccttgaagt tgtcgctggc ctcggacagc aagctgtgca cctcggcctt gcgcccggcg
     3181 cccgagacgc cggatgcgca gttggcgatc aggtgggcgc catcgaccag cttgtgcttg
     3241 agcagcggcg caaagcccag ctgcaccgaa gtcggatagc agcccggatt gccgacgacg
     3301 cgcgcctttt tgatggcttc gcggttcagc tcgggcaggc cgtagacggc ttcggccagc
     3361 agatccgggc agccgtgcgg catgccgtac cagcgctcga actcggccac gtccttgagg
     3421 cggaaatcgg ccgccaggtc gatgatcttg acgcccgcgg cgagcagctc gcgcgcctgg
     3481 gccatcgcca cgccatgcgg ggtcgcgaag aagaccacgt tgcattcggc gagcttggcg
     3541 tcgtcgggcg tcacgaaggc gagatcgaca tggccgcgca ggctcgggta catgtcggcc
     3601 accggcaagc ccgcttcttt gcgcgaggtg atggcacgca attcgacttg gccgtgctga
     3661 gacagcaggc gcagcaattc gaccccggtg tagccggtgc cgccgacgat gccgaccttg
     3721 atcatgttga ggctcgcgca gtaggagaac agggaagcct acgattgtag ggagcagcaa
     3781 tgaaaaggcc accttgcggt ggcctttgtg tcgttgcgtg tgcaacgaaa tgctgtcgtg
     3841 caggtatcag cgcttgctga actgcttgcg acggcgggcg ccgtgcaggc cgaccttctt
     3901 gcgctccact tcgcgggcgt cgcgggtcac gaagccggcg cggctcaact cggacttgag
     3961 cgccgcgtcg tagtcgatca gcgcgcgggt caccccatgg cgcacagcgc cggcctggcc
     4021 ggactcgccg ccaccgtgga cgttgacctt gatgtcgaag gcttcgacgt tgttcgtgag
     4081 gaacagcggc tgcttgacga tcatgatcga ggtctggcgg ccgaagtact gctccaccgg
     4141 cttgccgttg accgtgatct ggcccgtgcc cttcttgatg aacacacgag ccaccgacga
     4201 cttgcggcgg ccggtgccgt aattccaatt gcctatcatc gccgctcctt agacttccag
     4261 cgccttgggc tgctgagcgg tatgcgggtg cgtggcgccg gcatagacct tcagcttctt
     4321 gatcatcgcg tagcccagcg gacccttggg cagcatgccc ttgacggcct tctccagggc
     4381 acggcccgga tgcttggcct gcatgtcctt gaacttggtg ccgtagatgc cgcccgggaa
     4441 gccggaatgg cggtagtaca ccttttgctc ggccttgttg ccggtgacgc ggatcttgtc
     4501 ggcgttgacg atgacgatga agtcacccgt atcgacgtga ggcgtgtaaa tggctttgtg
     4561 cttgccacgc agacggagag cgacttcgct ggccactcgt ccgagcacct tatcggtggc
     4621 gtcaatcaca aaccattcgt gcttcacatc agcgggtttg gcgctgaagg ttttcataag
     4681 ctactcgaat ggatgcttgg ctctatctct actgagtacc gcggtcggtg cctcttctgt
     4741 tgcagcaccg gcgcattcgc gccaagctcg cagcggaggc ctcttaggcg gccgatgccg
     4801 gcgagagccg acaccccttc ccggagccaa atgcgggaaa gccgcaaatc ataccgcaga
     4861 atcaagcact taaccagact cgtctggtcc gctcggcgga ggaccatcgc aaaaacggga
     4921 tgccttccct gagtcagcgg ctatactgcg ggtaaaccct tatgcccatg ctagcccccg
     4981 ccatgaaacc gacgcaggac cacgcccc
//
LOCUS       DASUCY010000180         4176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1337233, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000180 DASUCY010000000
VERSION     DASUCY010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337233"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VFV25_03490"
     CDS             complement(<1..437)
                     /locus_tag="VFV25_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEY1392408.1"
                     /translation="MNTTTTTTSPTATSFAIGAPGLATRLFGQRSLYDWFFAMLVLSG
                     ATYAFASYGRVLDVYETGILIGAVPLLIGLGWFWGPLRHLAIAVAAASLAAIALYSRK
                     HGAYGADLAAGEQVFWLKYFLSSQSAILWMSVLFYMSTLFYWIG"
     gene            complement(441..2555)
                     /locus_tag="VFV25_03495"
     CDS             complement(441..2555)
                     /locus_tag="VFV25_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HEY1392409.1"
                     /translation="MTASTTGLELSPARSRHLREAVELVSSMRFAIALLTLICIASII
                     GTVVQQQQPAVNYVNQFGSFWASVFGTLRLDAIYSAPWFLLILAFLVISTSLCIVRNT
                     PKILHELRSFKEHLREQSLRAFHHKAEGELPESGEAALQRLSTLLAQEGWQAKAERRE
                     RGVMIAARKGMANKLGYLAAHSAIVLVCLGGLFDGDLIVRAQMWARGVTPYVGSGFVR
                     DVGAAHRLPASNPTFRGTLTVSEGQSSSIALLPVGEGVVLQELPFDIELRKFVVDFYA
                     TGMPKLFASDILIHDRDSGQVTAATVKVNQPVIHRGIAIYQSSFEDGGSHLKLRAYPM
                     GGMGQPFDVQGQVGGSTTLASAGGDASQQLALEFSGLRVINVENLGGDNSSTDVRKVD
                     LVGALEQHLGSGAKTTGKKELLNVGPSVSYKLRDAAGQAREYNNYMLPITLDGQRVFL
                     AGVRDTPADNFRYLRIPADEQGSMDGWLRLRRALADAAMREQAAQRYAALAMPDSGRP
                     ELTQQLRASALRALNLFAGVEPAREGAQPIGGLQAIADFMEANVPEADRARTSEVLVR
                     ILNGTLFELLNLSREQASLAPLKPGETTQAFMTSSVLSLSDAAFYPAPVLLALQDFKQ
                     VQASGFQVTRTPGKKLVYLGCLLLILGVFAMLYIRERRLWIWLQPAGHNTRVLTALSA
                     TRQTLDAGHEFERLKKAILQEA"
     gene            complement(2690..3340)
                     /locus_tag="VFV25_03500"
     CDS             complement(2690..3340)
                     /locus_tag="VFV25_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY1392410.1"
                     /translation="MKSFASLRASLLVAAVLAAPAALLSGNAQAAESAAAAKPDLKKG
                     EAISTQVCAACHTNDGSRGAPAYPILQGQHPEYIVKQLADFKSGARSNPIMSGMAAPL
                     SEDDMRNVAAFYAGKNAKPGFAKHKDLVKLGEKIYRGGIAGLNVPACAGCHSPDGAGI
                     PAQYPRLAGQQADYIEAQLLAFRDSVRKNSPQMRGVTLNLSDTDIKALADYIAGLR"
     gene            3389..4087
                     /gene="yihA"
                     /locus_tag="VFV25_03505"
     CDS             3389..4087
                     /gene="yihA"
                     /locus_tag="VFV25_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEY1392411.1"
                     /translation="MSPAPEPRSIQPTEPADAAADAKAALAWAHTARFLTTAAQLHQL
                     PVTELPEIAFVGRSNAGKSTAINTLTQQRQLAFASKTPGRTQHVNLFALGPRDEPDAL
                     LADLPGYGYARVERQAKLRWQEVMAQYLELRRNLAGVVQMIDSRHGYTDLDRQLLSLI
                     APRVGTGQVKLLVLLTKADKLNRKESAQAMRDAQHVLADVATDHADVGIALFSALKKS
                     GVDDAALALRSWIG"
BASE COUNT          669 a         1383 c         1456 g          668 t
ORIGIN      
        1 ccgatccagt agaacagcgt gctcatgtag aagagcacgc tcatccacag gatcgcactc
       61 tggctcgaca ggaagtactt gagccagaac acctgctcgc cggcggccag gtccgcgcca
      121 taggcgccgt gcttgcgcga atagagcgcg atcgctgcta ggctggctgc ggccaccgcg
      181 atggcgagat ggcgcagcgg gccccagaac cagcccagcc cgatcagcag cggcacggcg
      241 ccgatcagga tgccggtctc gtagacgtca agcacgcggc cgtaacttgc gaacgcatag
      301 gtcgcgccgc tcagcaccag catcgcgaag aaccagtcgt agagcgagcg ctgcccgaag
      361 agccgcgtcg ccagaccggg cgcgccgatg gcgaagctgg tggccgtggg gctagtggtc
      421 gtcgtggtgg tattcatgtc tcaggcctcc tggaggattg ccttcttgag ccgctcgaac
      481 tcatgaccgg catcgagggt ctggcgggtt gccgacagcg cggtcagcac gcgggtattg
      541 tggcccgccg gttggagcca gatccacagc cggcgttccc ggatgtagag catcgcaaac
      601 acgccgagga tgagcagcag gcagcccaga tagaccagct tcttgcccgg cgtgcgcgtc
      661 acctggaagc cgctggcctg gacctgcttg aaatcctgta gtgccagcag caccggggcc
      721 gggtagaagg cggcatcgga cagcgacagc accgagctcg tcatgaaggc ctgcgtcgtc
      781 tcgccgggct tgagcggcgc gaggctggct tgctcgcgcg acaggttgag cagctcgaac
      841 agcgtgccgt tgaggatgcg caccagcacc tcggacgtgc gcgcgcgatc ggcttcgggc
      901 acgttggctt ccatgaagtc ggcgatcgcc tgcaggccgc cgatcggctg ggccccttcg
      961 cgcgccggct ccacgccggc gaagaggttc agcgcccgca gcgccgacgc gcgcagctgc
     1021 tgggtcagct cgggccttcc gctgtcgggc atcgccagcg cggcgtagcg ctgcgcagcc
     1081 tgttcgcgca tggccgcatc ggccagtgcg cggcgcaggc gcagccagcc gtccatgctg
     1141 ccctgctcgt cggccgggat gcgcagatag cggaaattgt cggccggcgt gtcgcgcacg
     1201 ccggcgagga agacgcgctg cccgtccagc gtgatcggca gcatgtagtt gttgtactcg
     1261 cgtgcctggc cggcggcgtc gcgcagcttg tagctcaccg aggggccgac gttgagcagc
     1321 tccttcttgc ccgtcgtctt ggcgcccgag cccagatgct gctccagagc gccgaccagg
     1381 tcgaccttgc gcacatcggt gctgctgttg tcgccgccga ggttctcgac attgatgacg
     1441 cgcagcccgc tgaattcgag cgcgagctgc tggctggcgt cgccgcctgc gctggccagc
     1501 gtcgtcgaac cgcctacctg cccctggaca tcgaagggct ggcccatgcc gcccatcgga
     1561 taggcgcgca gcttcaagtg cgagccgccg tcctcgaagc tggactggta gatcgcgatg
     1621 ccgcggtgga tcaccggctg gttgaccttg accgtggccg ccgtcacctg gccgctgtcg
     1681 cggtcgtgga tcaggatgtc gctggcgaag agcttgggca tgccggtggc atagaaatcg
     1741 acgacgaact tcctgagctc gatgtcgaag ggcagctcct gcagcaccac gccttcgccc
     1801 accggcagca gggcgatgct ggagctctgg ccttcgctga ccgtgagcgt gccgcggaag
     1861 gtcgggttgc tcgccggcag gcggtgcgcg gcgcccacgt cgcgcacgaa gccgctgccc
     1921 acatagggcg tgacgccgcg cgcccacatc tgggctcgca cgatcaggtc gccgtcgaac
     1981 aggccaccca ggcagaccag cacgatggcg ctgtgggcgg ccagatagcc cagcttgttc
     2041 gccatgccct tgcgggccgc gatcatcacg ccccgctcac gccgctcggc cttggcctgc
     2101 cagccctcct gcgccagcag ggtcgacagg cgctgcagag cggcctcgcc cgattcgggg
     2161 agttcaccct cggccttgtg atggaaggcg cgcaggctct gctcgcgcag atgctccttg
     2221 aagctgcgca gctcgtgcag gatcttgggc gtgttgcgga cgatgcacag gctggtggag
     2281 atcaccagga aggccaggat cagcaggaac cagggcgcgc tgtagatcgc gtccagccgc
     2341 agcgtgccga acaccgaagc ccagaagctg ccgaactggt tgacgtagtt gaccgccggc
     2401 tgctgctgct ggaccaccgt gccgatgatg gaggcgatgc agatcagcgt gagcagcgcg
     2461 atcgcgaagc gcattgacga gaccagctcg accgcttcgc gcagatgccg cgagcgcgcg
     2521 ggggagagtt cgaggccggt ggtgctggcg gtcatggcgg gattatcgga agctgtgcga
     2581 gggcggtgct gagccagctc aaagccaaaa aagcaaaaag gccggtcgcg gcagcaaccg
     2641 gcctttgctc atggcggctc gcccgggcct tggagcaggc cctcagggct cagcgcaggc
     2701 cggcgatgta gtcggccagg gccttgatat cggtatcgct caggttgagc gtgacgccgc
     2761 gcatctgcgg gctgttcttg cgcacgctat cgcggaaggc cagcagctgg gcctcgatgt
     2821 agtcggcctg ctggccggcc aggcgcggat actgggccgg gatgccggcg ccgtccgggc
     2881 tgtggcagcc ggcgcaggcc ggcacgttca gtcccgcgat gccgccgcgg tagatcttct
     2941 cgcccagctt gaccaggtcc ttgtgcttgg cgaagccggg cttggcgttc ttgccggcgt
     3001 agaaggcggc gacgttgcgc atgtcgtctt cgctcagcgg cgcggccatg ccggacatga
     3061 tggggttgct gcgcgcgccg gacttgaagt cggcgagctg cttgacgatg tactcggggt
     3121 gctggccctg caggatgggg taggccgggg cgccgcggct gccgtcgttg gtgtggcagg
     3181 ccgcgcagac ctgggtcgag atcgcctcgc ccttcttgag atcaggcttg gcagcggctg
     3241 cagactcggc ggcctgggcg ttgccgctca acagagcggc cggggctgcc agcacggcag
     3301 ccactagcaa ggaagcgcgc aaggaggcga aggacttcat ggcgttggcc catttattgt
     3361 gcaaaaccca gcaattttac aatgccccat gagtccagct cccgaacccc gttccataca
     3421 gcccactgag cccgcggacg ccgctgccga tgcaaaggcc gccctggcct gggcccacac
     3481 ggcgcgcttc ctgaccacgg cggcccagct gcaccagctg cccgtcaccg agctgcccga
     3541 gattgccttt gtcggccgct ccaacgccgg caagtccacc gcaatcaaca cgctgaccca
     3601 gcagcgccag ctcgccttcg catccaagac gcccgggcgt acccagcacg tcaacctgtt
     3661 cgcgctcggc cccagggacg agcccgacgc actgctggcc gacctgcccg gctacggcta
     3721 cgcgagggtg gagcgccagg ccaagctgcg ctggcaggag gtgatggctc agtacctgga
     3781 gctgcgccgc aatctcgccg gcgtggtgca gatgatcgat tcgcgccacg gctacaccga
     3841 tctggaccgc cagctgctca gcttgatcgc gccgcgcgtg ggcaccggcc aagtcaagct
     3901 gctggtgctg ctcaccaagg ccgacaagct caatcgcaag gagagcgcgc aggcgatgcg
     3961 cgatgcccag cacgtgctgg ccgatgtcgc caccgaccat gccgacgttg gcatcgcgct
     4021 gttttcggcg ctgaagaagt ccggtgtgga cgacgcggcg ctggcgctgc gcagctggat
     4081 tggatgacgg cacccggtcg cggatgaccg cgaccaccct ccgcgagggt gccggtttgc
     4141 ttgggacggc ccggcgcggc gccggcctag ctggag
//
LOCUS       DASUCY010000181         2472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1048102, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000181 DASUCY010000000
VERSION     DASUCY010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048102"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1105
                     /locus_tag="VFV25_03510"
     CDS             <1..1105
                     /locus_tag="VFV25_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEY1392412.1"
                     /translation="GVVKNITEYGAFVDLGGIDGLLHITDMAWRRVRHPSEVVTVGQE
                     LTAKVLKFDAEKNRVSLGLKQMGDDPWVGVSRRYPTGTRLFGKVTNIADYGAFVEIEP
                     GIEGLVHVSEMDWTNKNVAPAKIVSLGDEVEVMVLEIDEDKRRISLGMKQCKPNPWDE
                     FAQNHKRGDKLRGPVKSITDFGVFVGLAAGIDGLVHLSDLSWNEPGEAAVRNYKKGQE
                     VDTVVLAIDVERERISLGIKQLDADPFTNYTAVNDRGMTVSGTVKSVDARGAEIQLSD
                     EVTGYLRASEISRDRVEDARNVLKEGDEVTALIVNIDRKTRSIQLSIKAKDSADQQEA
                     MQRLSSESSRENAGTTSLGALLKAKLDNQNNNG"
     gene            1183..1527
                     /locus_tag="VFV25_03515"
     CDS             1183..1527
                     /locus_tag="VFV25_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEY1392413.1"
                     /translation="MTRSDLVALLAERFGQLTHRDTEFAVKTILDAMSDALARGHRIE
                     IRGFGSFSINRRPPRVGRNPRSGEQVIIPEKLVPHFKPGKALREAVDEQVPVLDDDAT
                     AELKPAKSKTPA"
     gene            1581..1895
                     /locus_tag="VFV25_03520"
     CDS             1581..1895
                     /locus_tag="VFV25_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HEY1392414.1"
                     /translation="MRVLTWLLRAAVFFALFAFALNNQQEAELQWFFGHQWRAPMVFI
                     VLTAFALGCAFGVLAMVPSWWRHRRIAQRHHRPATPVAGETIAPPTPGGIVLEHPPRD
                     GL"
     gene            1885..>2472
                     /locus_tag="VFV25_03525"
     CDS             1885..>2472
                     /locus_tag="VFV25_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HEY1392415.1"
                     /translation="MDFDPYWLLLGLPIVFALGWLASRLDVRQWKREQSESPKAYYKG
                     LNLLLNEQQDKAIDAFIEAVQQDPGPSDLHFALGNLFRRRGEYERAVRVHQHLLSRAD
                     LAPAERERAQHALAQDYMKAGLFDRAEEAFRSLEGTAFATDAKLARLTLYERSRDWHA
                     AVETARQLEAAGAGSFASRIANYWCEIALEADARKQ"
BASE COUNT          455 a          882 c          778 g          357 t
ORIGIN      
        1 tggcgtggtc aagaacatca ccgagtacgg cgccttcgtc gacctcggcg gcatcgacgg
       61 cctgctgcac atcaccgaca tggcatggcg ccgcgtgcgt catccgagcg aggtggtgac
      121 ggtcggccag gaactcaccg ccaaggtgct gaagttcgac gccgagaaga accgcgtctc
      181 gctgggcctg aagcagatgg gcgacgaccc ctgggtcggc gtgtcgcgcc gctaccccac
      241 cggcacccgt ttgttcggca aggtcaccaa catcgccgac tacggcgcct tcgtcgagat
      301 cgagccgggc atcgagggcc tggtgcacgt ctccgaaatg gactggacca acaagaacgt
      361 cgcgccggcc aagatcgtct cgctgggcga cgaggtcgag gtcatggtgc tcgagatcga
      421 cgaggacaag cgccgcatca gcctcggcat gaagcagtgc aagcccaacc cctgggacga
      481 gttcgcgcag aaccacaagc gcggcgacaa gctcaggggc ccggtcaagt cgatcaccga
      541 cttcggcgtc ttcgttggcc tggccgccgg catcgacggc ctggtgcacc tgtccgacct
      601 gtcctggaac gagccgggcg aagccgccgt gcgcaactac aagaagggcc aggaagtcga
      661 caccgtcgtg ctggccatcg acgtggagcg cgagcgcatc tccctgggca tcaagcagct
      721 cgacgccgac cccttcacca actacacggc cgtcaatgac cgcggcatga ccgtgagcgg
      781 caccgtgaaa tcggtggacg cccgtggcgc cgagatccag ctcagcgacg aggtgaccgg
      841 ctacctgcgt gcttccgaga tcagccgcga ccgcgtcgaa gacgcccgca acgtgctgaa
      901 ggaaggcgac gaagtcacgg ccctgatcgt caacatcgac cgcaagacgc gctcgatcca
      961 gttgtcgatc aaggccaagg acagcgccga ccagcaggaa gccatgcagc gcctgtccag
     1021 cgaaagcagc cgcgagaacg ccggcaccac cagcctgggc gccctgctca aggccaagct
     1081 cgacaaccag aacaacaacg gttgatgagc gcgcagcggg aaccgcgtgg cggttcccgc
     1141 tgttttccct gaacactcct tcgacccaga tcccaggacg acatgacccg ctcagatctc
     1201 gtggccctgc tcgccgaacg cttcggccag ttgacccatc gcgataccga gttcgccgtc
     1261 aagaccatcc tggacgccat gtccgacgcg ctggcgcgcg gacaccgcat cgagatccgc
     1321 ggcttcggca gcttctccat caaccggcgt ccgccgcgcg tgggccgcaa cccgcgttcg
     1381 ggcgagcagg tcatcattcc cgagaagctc gtgccccatt tcaagcccgg caaggcgctg
     1441 cgcgaggcag tcgacgagca ggttcccgtg ctcgacgacg acgcaaccgc ggaactgaag
     1501 ccagcaaaga gcaagacgcc cgcttgagca gggcgctgct ggcgctgggc gccacgccac
     1561 tctatagaat ccgcgctgcc atgcgcgtcc tcacctggct cctgcgagcc gccgtcttct
     1621 tcgccctctt cgctttcgcg ctgaacaatc agcaggaagc ggagctgcaa tggttcttcg
     1681 gccatcaatg gcgagcgccc atggtcttca tcgtgctgac ggccttcgcg ctcggctgcg
     1741 ccttcggcgt gctggcgatg gtgccgagct ggtggcgcca tcgccgcatc gcgcagcgcc
     1801 accaccgccc cgcaacgccg gtggccggcg agaccatcgc gccgcccacc cctggaggca
     1861 tcgtcctgga acacccgccg cgcgatggac tttgatccgt attggctgct gctcggcctg
     1921 ccgatcgtct tcgcgctggg ctggctggcc tcgcgcctgg acgtgaggca gtggaagcgc
     1981 gaacagagcg aatcgccgaa ggcctactac aagggcctga acctgctgct caacgagcag
     2041 caggacaagg cgatcgacgc cttcatcgag gcggtgcagc aggaccccgg cccctccgac
     2101 ctgcacttcg cgctcggcaa cctgttccgc cgtcgcggcg aatacgaacg cgccgtgcgc
     2161 gtgcaccagc acctgctcag ccgcgccgac ctcgcacccg ccgagcgcga gcgcgcccag
     2221 cacgccctgg cccaggacta catgaaggcc ggtctgttcg accgcgccga agaggccttc
     2281 cgcagcctgg aaggcacagc cttcgccacc gatgcgaagc tggcgcgcct cacgctctac
     2341 gagcgctcgc gcgactggca tgcggcggtc gagaccgcgc gccagctcga ggccgccggc
     2401 gccggctcct tcgccagccg catcgccaac tactggtgcg agatcgcgct ggaggccgac
     2461 gcgcgcaagc ag
//
LOCUS       DASUCY010000182         2188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_518119, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000182 DASUCY010000000
VERSION     DASUCY010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518119"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1132
                     /gene="dnaJ"
                     /locus_tag="VFV25_03530"
     CDS             <1..1132
                     /gene="dnaJ"
                     /locus_tag="VFV25_03530"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEY1392416.1"
                     /translation="YEILGVAKNASEEDIKKAYRKSAMKHHPDRNQGDDAKKAEEKFK
                     EAKEAYEMLSDPKKRAAYDQYGHAGVDPNMGAGGFRGGPEGFGGFAEAFGDIFGDIFG
                     GSAGAGRRGGGHQVYRGSDLSYAMEITLEEAAHGKDTQIRIPSWDECGTCHGTGAKPG
                     TSAKTCPSCHGSGTVHLRQGFFSIQQTCPHCHGSGKIIPDPCTTCGGQGRIKQNKTLE
                     VKIPAGINDGMRIRSAGNGEPGMNGGPAGDLYIEIHIKPHEIFERDGDDLHCQVPVGM
                     TLAALGGSIEVPTLSGKAEIDIPEGTQHGKTFRLRGKGIKGLRSSYPGDLYCHMAVET
                     PVKLTEHQRKLLKELEESLKKGGDRHSPNAKTWSDRVKDLFK"
     gene            complement(1260..>2188)
                     /locus_tag="VFV25_03535"
     CDS             complement(1260..>2188)
                     /locus_tag="VFV25_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HEY1392417.1"
                     /translation="EEHVKSILARNDSPDIPFELSLNPYRGCEHGCIYCYARPTHSYL
                     NLSPGLDFETRIFAKVNAAQRLEEELGRRGYLPSPINIGSVTDAYQPIERKLGITRAV
                     LELLTSCEHPFTIITKSSGVERDLDLIAPAARRHQAVIFVSITTLDSALSRILEPRAA
                     APQRRLQTVQRLSEAGVPVGVNIAPIIPFINEPEIERIAEAVAQAGARSLHWTVLRLP
                     WEVAPLFRHWLDQHFPRRAARVMARVQEMRGGHDYKADFVTRMKGEGVWAGLIRQRIE
                     KAAARHGLQRGRYELDCSRFLAPQALAQGQLF"
BASE COUNT          424 a          711 c          732 g          321 t
ORIGIN      
        1 ctacgaaatc ctcggcgtcg ccaagaacgc gagcgaagag gacatcaaga aggcttatcg
       61 caagtcggcg atgaagcatc accccgaccg caaccagggg gacgatgcca agaaggccga
      121 ggagaagttc aaggaggcca aggaggccta cgagatgctc tcggacccca agaagcgcgc
      181 ggcctacgac cagtacggcc atgcgggggt cgatcccaac atgggcgccg gcggcttccg
      241 cggcggcccg gaaggcttcg gcggcttcgc cgaggccttc ggtgacatct tcggcgacat
      301 cttcggtggc agcgcaggcg ccggccggcg cggcggcggg catcaggtct accgcggcag
      361 cgacctctcc tacgcgatgg agatcacgct ggaggaagcg gcgcacggca aggacacgca
      421 gatccgcatc ccgagctggg acgaatgcgg cacctgccac ggcacgggcg ccaagccggg
      481 caccagcgcc aagacctgcc ccagctgcca cggctcgggc acggtgcacc tccgccaggg
      541 cttcttctcg atccagcaga cctgcccgca ttgccatggc agcggcaaga tcattccaga
      601 tccctgcacc acgtgcggcg gccagggccg catcaagcag aacaagacgc tggaggtgaa
      661 gatcccggcc ggcatcaacg acggcatgcg catacgctcg gccggcaacg gcgagcctgg
      721 catgaacggc gggccggcgg gcgatctcta catcgagatc cacatcaagc cgcatgagat
      781 cttcgagcgc gacggcgacg acctgcactg ccaagtgccg gtcggcatga cgctggccgc
      841 gctgggcggc agcatcgaag tgcccacgct gtccggcaag gccgagatcg acatccccga
      901 aggcacgcag cacggcaaga ccttccggct gcgcggcaag gggatcaagg gcctgcgctc
      961 cagctatccc ggcgacctgt actgccacat ggcggtcgaa accccggtca agctcaccga
     1021 gcaccagcgc aagctgctca aggagctgga ggagtcgctg aagaaaggcg gcgaccggca
     1081 ttcgcccaat gccaagacct ggagcgatcg ggtcaaggat ttgttcaagt aggaatagcg
     1141 gcggctcgaa ccgggccccc gcagcgcaat gccacgaggc ttggttcgat acatatttag
     1201 tactgcattt ccttgaaaac tctagcgcaa tccataggaa agttgctgaa attccggttt
     1261 cagaacaact gcccttgtgc cagcgcctga ggcgccaaga agcggctgca gtccagctcg
     1321 tagcgcccgc gctgcagccc atggcgcgcc gcggccttct cgatgcgctg gcggatcagc
     1381 cccgcccaca cgccctcgcc tttcatgcgc gtcacgaagt cggccttgta gtcgtggccg
     1441 ccgcgcatct cctgcacgcg tgccattaca cgggcggcgc ggcgcgggaa atgctggtcc
     1501 agccaatgcc tgaagagcgg cgcaacctcc cagggcaggc gcagcaccgt ccaatgcagc
     1561 gagcgggcgc cggcttgggc cacggcctcg gcgatgcgct cgatctcggg ctcgttgatg
     1621 aagggaatga tgggcgcgat gttgacgccc accggcacgc ccgcctcgct caggcgctgc
     1681 acggtttgca gccggcgctg cggcgccgcg gcgcgcggct ccaggatgcg cgacagcgcc
     1741 gagtcgagcg tcgtgatgct gacgaagatc accgcctggt gccgccgtgc cgccggcgcg
     1801 atgagatcga ggtcgcgctc gacgcccgag gacttggtga tgatggtgaa gggatgctcg
     1861 caactggtga gcagctccag cacggcgcgc gtgatgccca gcttgcgctc gatgggctgg
     1921 taggcatcgg tcaccgaacc gatgttgatc gggctgggca ggtagccgcg ccgccccagt
     1981 tcttcctcaa ggcgctgcgc cgcgttgacc ttggcgaaga tcctggtctc gaagtccaac
     2041 cccggcgaga ggttgagata actgtgcgtg ggccgcgcat agcagtagat gcaaccatgc
     2101 tcgcagcctc ggtaagggtt caacgaaagc tcgaagggaa tgtcgggcga atcgttgcgc
     2161 gcgaggatgg acttgacgtg ctcctcgg
//
LOCUS       DASUCY010000183         2165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_871391, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000183 DASUCY010000000
VERSION     DASUCY010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871391"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VFV25_03540"
     CDS             <1..228
                     /locus_tag="VFV25_03540"
                     /inference="COORDINATES: protein motif:HMM:NF017733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HEY1392418.1"
                     /translation="LPFEGPLTFGTGIDIALELDELAFQGASAFLFASVLERFFARHA
                     AINSFIQLRLNTAQRGQVMRWPPRLGLGETV"
     gene            225..1301
                     /gene="tssG"
                     /locus_tag="VFV25_03545"
     CDS             225..1301
                     /gene="tssG"
                     /locus_tag="VFV25_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HEY1392419.1"
                     /translation="MSADRQAAVQSLFGALQAAPHAHDFFALLRRIEALHPEAPRFGQ
                     ALRPSQEALRLGQEPELDFAPAALASFEPHGAHAPRLGVRFFGLLGPQGPLPLHLTEY
                     ARERARQRGDPTFARFLDVFHHRLLSLFYRAWAQGQPTVQHDRPQDDRFAAWLGACFG
                     HGAGLSRQGSLGGGSRLFQAGLLASRSRHPEGLAKILALHFDIPARIQQYVPHWMDLE
                     REDRCRLGHARSRSRQGLDAGAPARLGMTATSGRKAWDRQYKFRIVLGPLTLAQYRAL
                     LPGGAAWRELRDWVLQYVGLDLEWDVELCLARNEVPQPHLGRQVQLGLTGWLGRRRTQ
                     RDASDLRLRPRHSFLLHRQGASHV"
     gene            1294..>2165
                     /locus_tag="VFV25_03550"
     CDS             1294..>2165
                     /locus_tag="VFV25_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HEY1392420.1"
                     /translation="MSEISRTALFGKLNALGYKAIEGATVFCKLRGNPYVELQHWIYQ
                     ILNTQDSDLHRIVKHYGLDASVLAGDLTASLDRLPRGASSVTDLSSWVENAVERGWVY
                     GSLMFSDTQVRTGYLVVGLLKTASMRNALLSISRQFERIKLDDLTENFARILAGSPEH
                     GQPASDGSGAAEPGEASGAIAPAAMGKQEALKRYAVDLTERARSGEIDPVAGRDEEIR
                     QIVDILMRRRQNNPILTGEAGVGKTAVVEGFALRLAQGDVPPQLKDVSLYALDIGLLQ
                     AGASMKGEFENRLRS"
BASE COUNT          323 a          759 c          747 g          336 t
ORIGIN      
        1 ctgcccttcg aggggccgct caccttcggc accggcatcg acatcgcgct ggagctggac
       61 gaactcgcct tccagggcgc gagcgccttc ctgttcgcga gcgtgctgga gcggttcttc
      121 gcacgccatg cggcgatcaa cagcttcatc cagctcaggc tgaacacggc gcagcgcggc
      181 caagtgatgc gctggccgcc ccgcctgggt ttgggggaaa cggtgtgagc gccgaccgcc
      241 aggctgctgt gcagagcctg ttcggcgcgc tgcaagccgc gccgcatgcc catgatttct
      301 tcgcactgtt gcgacggatc gaggctttgc accccgaagc gccgcgcttc ggccaagcgc
      361 tgcggccttc gcaggaggcg ctgcgcctgg ggcaggaacc tgagctggac ttcgcccccg
      421 ctgcgctcgc aagcttcgag ccgcacgggg cccatgcgcc gcgcctgggc gtgcgtttct
      481 tcgggctgct ggggccgcag gggcccttgc cgctgcacct gaccgaatac gcgcgcgagc
      541 gtgcgcgcca gcgcggcgat ccgaccttcg cgcgctttct cgatgtcttt caccaccgcc
      601 tgctgtcgct cttctatcga gcctgggccc agggtcagcc cacggttcag cacgaccgcc
      661 cgcaggacga ccgcttcgcc gcctggctgg gcgcctgctt cgggcacggc gcaggcttaa
      721 gccgccaggg ttcgctcggc ggcggcagcc gcttgttcca ggccgggctg ctcgcctcgc
      781 gcagccgcca tcccgaaggc ctggccaaga tcctggccct gcacttcgac atacccgcgc
      841 gcatccagca atacgtgccg cactggatgg atctggagcg cgaggatcga tgccgcctag
      901 gccatgctcg cagccggagc cggcaaggcc tcgatgccgg cgccccggcg cggctgggga
      961 tgacggctac ctcgggacgc aaggcctggg atcggcaata caagttccgc atcgtgctcg
     1021 gcccgctgac gctggcgcaa taccgggccc tgctgccggg cggcgcggcc tggcgggagc
     1081 tgcgagattg ggtgctgcag tacgtgggcc tggacctcga gtgggacgtc gagctctgcc
     1141 tggcgcgcaa cgaagtcccg cagccgcatc tgggccgcca ggtgcaactg ggcctcaccg
     1201 gctggttagg ccggcgccgc acgcagcgcg acgcgagcga cctgaggctg agaccccggc
     1261 actcgttcct gctgcatcga cagggagcaa gccatgtctg agatctcgcg caccgccttg
     1321 ttcggcaagc tcaacgccct gggatacaag gccatcgaag gggccacggt gttctgcaag
     1381 ctgcgcggca acccctatgt ggagctgcag cactggatct accagatcct caacacccag
     1441 gattccgacc tgcaccgcat cgtcaagcat tacgggctcg acgcctctgt gttggccggc
     1501 gacctgacgg cctcgctcga ccgcctgccg cgcggcgcct cgtcggtgac cgatctgtcc
     1561 agctgggtcg agaacgcggt cgagcgcggc tgggtctacg gctcgctgat gttcagtgac
     1621 acgcaggtgc gcaccggcta cctggtcgtg ggcctgctca agacggcatc gatgcgcaat
     1681 gccttgctct cgatttcccg acagttcgag cgcatcaagc tcgacgacct caccgagaac
     1741 ttcgcgcgca tcctcgccgg ctcgcccgag catggccagc ccgccagcga cggcagcggg
     1801 gcggccgagc ccggcgaggc gagcggcgcc atcgcgccgg ccgcgatggg caagcaggag
     1861 gcgctcaagc gctatgcggt ggacctgacc gagcgggcac gcagcggcga gatcgacccg
     1921 gtggccgggc gcgacgagga gatccgccag atcgtcgaca tcctgatgcg ccggcgccag
     1981 aacaacccca tcctcaccgg cgaggcgggc gtgggcaaga cggcggtggt ggagggcttt
     2041 gcgctgcggc tggcgcaggg cgacgtgccg ccgcagctga aggacgtgtc gctgtacgcg
     2101 ctggacatcg gcctgctgca ggccggcgcc agcatgaaag gcgagttcga gaaccggctg
     2161 cgctc
//
LOCUS       DASUCY010000184         6601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_967856, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000184 DASUCY010000000
VERSION     DASUCY010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 6601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6601
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967856"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..136)
                     /locus_tag="VFV25_03555"
     CDS             complement(<1..136)
                     /locus_tag="VFV25_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEY1392421.1"
                     /translation="MARAPSDGLVTPEYWDEACKHLGKRDRVMRKLIPQFGEARLQSR
                     G"
     gene            complement(147..1523)
                     /gene="cysS"
                     /locus_tag="VFV25_03560"
     CDS             complement(147..1523)
                     /gene="cysS"
                     /locus_tag="VFV25_03560"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_036235430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEY1392422.1"
                     /translation="MTLRIHNTLTRKTEDFVPIEPGHVRMYVCGMTIYDLCHVGHARM
                     MMAFDVIQRWLKVSGYRVNYVRNITDIDDKIIKRALERGITIRQLTEEMTAAMHQDIG
                     ALGIEMPTHEPRATEYVKDMVGMISALEAKGLAYRAPNGDVNFAVRKFPGYGKLSGKS
                     LDQLRAGERVAVLEGKDDPLDFVLWKAAKPEEPADAKYDAPFGAGRPGWHIECSAMSC
                     ALLGENFDIHGGGMDLQFPHHENEIAQSEGASGKPFVNYWLHNGFLNVDNEKMSKSLG
                     NFFTIRDVLKSYDGETIRFFMLRVHYRSPFNYSDAGLEDARHALRRLYTALGSVKAAE
                     AKAVDWNEPHGARFKAAMDDDFNTPIAVSVLFELAGEVNRSGSVELASQLKALGATLG
                     LLQQSPQAYLQGGAAIDEAAIQAGIEARAAAKKARNFAEADRIREALAAQGIVLKDTP
                     AGTTWVKA"
     gene            1781..2323
                     /locus_tag="VFV25_03565"
     CDS             1781..2323
                     /locus_tag="VFV25_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY1392423.1"
                     /translation="MLAILAGLGLSDLTLAQKVKLATTAGDIVVQLDRTKAPKTVDNF
                     LQYVKAGHYDGTIFHRVIPNFMIQGGGMTPDLNEKPTRPPIALESRNGLKNERGTIAM
                     ARTPDPDSAAAQFFINVKDNAFLDQSNARDGYGYAVFGKVIKGMDVVDKIKQVPTGNR
                     GEHQNVPLQPVVITKATVEK"
     gene            2326..2829
                     /locus_tag="VFV25_03570"
     CDS             2326..2829
                     /locus_tag="VFV25_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY1392424.1"
                     /translation="MSKTIELHTNLGVIRAELDDAKAPKTVANFLAYAGKGHYDGTVF
                     HRVIKGFMVQGGGFEPGLKQKPTDAAIENEANNGLKNEKYTLAMARTSAPHSATAQFF
                     INTANNEFLNFKSESSQGWGYAVFGKVVAGADIVDQIEKVRTGRSGFHDDVPLEDVVI
                     TKVVEVA"
     gene            2832..3629
                     /locus_tag="VFV25_03575"
     CDS             2832..3629
                     /locus_tag="VFV25_03575"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEY1392425.1"
                     /translation="MITPAAAAPLPHLSDYSAPAGWRSIDFLSDLHLQGSTPRSVEAL
                     ARHLEQTSADAVFILGDLFEAWPGDDARFDGFEQQCAAMLREAAARKTVGFMCGNRDF
                     LVGTALLQDCGMLALPDPTVLIAFGRRILLIHGDALCLADHDYQRFRTQVRNPAWQAG
                     FLAKPLSERRAIAQQMRAQSEQHKREQGRDNWIDLDRDATLQWMEAADTPTLIHGHTH
                     HPSHERLAPGRERWVLSDWDLDHEPRRADVLRLDAAGIRRIDIATAA"
     gene            3626..4423
                     /locus_tag="VFV25_03580"
     CDS             3626..4423
                     /locus_tag="VFV25_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HEY1392426.1"
                     /translation="MIGSWWRRRREARVLAQRAIPEPLWQTTLALYPFLQLADPAEQQ
                     ELRRLTSLFLAQKEFTGAGGLIVTDAMAVAVAAQACLPILHLGLSSYAGFVGIVIHPD
                     EVVAKRETMDEAGVVHHYDEVLAGEAMEGGPVMLSWRDVAGAGESAAQGYNVVIHEFV
                     HVLDMADGRPPDGIPPLASRAAREHWAMVLAAEYERFCHAVDAGYETFLDPYGTTGPE
                     EFFSVAAEAFFVAPAELQQEHRYMYELMAHYFKQDPAQRAAQKERGA"
     gene            complement(4463..>6601)
                     /gene="clpA"
                     /locus_tag="VFV25_03585"
     CDS             complement(4463..>6601)
                     /gene="clpA"
                     /locus_tag="VFV25_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEY1392427.1"
                     /translation="ASLTNFIKDNTPQVAGTDDVDTQPTLGFQRVIQRAIMHVQSTGS
                     GKKEVTGANVLVAIFGEKDSHAVYYLHQQGVTRLDVVNFIAHGIKKSEPPEPAKSNDG
                     AGSEGEKEEGEAKGSPLEQFTQNLNQLAKDGKIDPLIGRELEVERVIQVLCRRRKNNP
                     LLVGEAGVGKTAIAEGLAWRITQGEVPEVLADATVYALDMGALLAGTKYRGDFEQRLK
                     GVLKQLKEQPGAVLFIDEIHTLIGAGAASGGTLDASNLLKPALSTGALKCIGATTFSE
                     YRGIFEKDAALSRRFQKIDVVEPSVEQTVEILKGLKSRFEEHHSVKYAVTALQAAAEL
                     SAKFINDRHLPDKAIDVIDEAGAAQRILPKNKQKKTITRTEVEEIVAKIARIPPASVS
                     NDDRSKLKNLDRDLKSVVFGQDVAIDALAAAIKMARSGLGKPDKPIGSFLFSGPTGVG
                     KTEVAKQLAYILGIELIRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTEAITKKPH
                     AVLLLDEIEKAHPDVFNVLLQVMDHGTLTDNNGRKADFRNVIIIMTTNAGAETMNKAT
                     IGFTTAREQGDEMADIKRLFAPEFRNRLDAIVSFRSLDEEIILRVVDKFLLQLESQLA
                     EKKVEVTFTDALRKHLAKKGFDPLMGARPMQRLIQDTIRRALADELLFGRLVDGGRLT
                     VDIDDKEQPVLDIQPARKSDRPKAEAATTD"
BASE COUNT         1073 a         2218 c         2114 g         1196 t
ORIGIN      
        1 cgccgcggct ttgcagcctg gcctcgccga actgggggat gagcttgcgc atcacgcggt
       61 cgcgcttgcc gagatgcttg caggcctcgt cccagtattc gggcgtgacc aggccgtcgg
      121 aaggagcacg tgccacgttc aagcccttac gctttcaccc aggtggtccc ggcaggcgtg
      181 tccttgagca cgatgccctg cgcggccagc gcctcgcgaa tgcgatcggc ctcggcgaaa
      241 ttcctggcct tcttggccgc ggcgcgagcc tcgatgccgg cctgtatggc cgcctcgtcg
      301 atcgccgcac cgccctgcag ataggcttgg gggctttgct gcagcaagcc cagcgttgca
      361 cccagggcct tcagctggct cgccagctcg accgagccgc tgcggttgac ctcgcccgcc
      421 agctcgaaca gcacggacac ggcgatcggc gtattgaagt cgtcgtccat cgcggccttg
      481 aagcgcgccc catggggctc gttccagtcg acggccttcg cttcggcagc cttcacgctg
      541 cccagcgccg tgtagagccg ccgcagagca tggcgcgcat cctccaggcc ggcgtcgctg
      601 tagttgaagg ggctgcgata gtgcacgcgc agcatgaaga agcggatcgt ctcgccgtcg
      661 tagctcttca gtacgtcgcg gatggtgaag aagttgccga gcgacttcga catcttctcg
      721 ttgtcgacgt tgagaaagcc gttgtgcagc cagtagttca cgaagggctt gccgctcgcg
      781 ccttcgctct gcgcgatttc gttttcgtga tgcgggaact gcagatccat gccgccgccg
      841 tggatgtcga agttctcgcc gagcagggcg cagctcatcg ccgaacattc gatgtgccag
      901 cccggacggc cggccccgaa gggggcgtcg tacttcgcat cggccggctc ctcgggcttg
      961 gcggccttcc acagcacgaa gtccagcggg tcgtccttgc cttcgagcac ggccacgcgc
     1021 tcgccggcac gcagctggtc gagcgacttg ccggacagct tgccgtagcc gggaaacttg
     1081 cgcaccgcga agttcacgtc gccattgggc gcacgatagg ccaggccctt ggcctccagc
     1141 gcgctgatca tgccaaccat gtccttgacg tactcggtgg cgcgcggctc atgcgtgggc
     1201 atctcgatgc cgagcgcgcc gatgtcctgg tgcatcgccg ccgtcatttc ttcggtgagc
     1261 tggcgtatcg tgatgccgcg ctccagcgcg cgcttgataa tcttgtcgtc gatgtcggtg
     1321 atattgcgca catagttgac gcggtagccg ctcaccttga gccagcgctg gatcacgtcg
     1381 aaggccatca tcatgcgagc atggccgacg tgacacaggt catagatcgt catgccgcac
     1441 acgtacatgc gcacgtggcc cggctcgatg ggcacgaagt cttcggtctt gcgcgtcagc
     1501 gtgttatgaa tgcgcaacgt catcgggaat cgaaaagctt gggggaaaga gacctgcagc
     1561 gcgctgatgc cgccgcggac tccagggctg tgtcgcctgg gccctcagat agaatttccg
     1621 cagtatatct gcgcgcctct ctttgtctca ttggcgcaat gcaccgttta tccgtgcatt
     1681 tccctatcgc gctgttcgtc cgcgcattct gcgtcgcgcc gtttagcgac gaccttgaag
     1741 gagcccatcg atgaaacgtt tcctgtcctg gctgccagcg atgctcgcga tcctggcggg
     1801 cctgggtctg tccgatctga ccctggccca gaaggtcaag ctcgcgacca cggccggcga
     1861 catcgtggtc cagctcgacc ggaccaaggc gcccaagacc gtcgacaact tcctgcaata
     1921 cgtcaaggcc ggacactatg acggcacgat cttccaccgc gtgatcccca acttcatgat
     1981 ccagggcggc ggcatgacgc ccgacctgaa cgagaagccc acgcgcccgc cgatcgcgct
     2041 ggaaagccgc aacggcctga agaacgagcg cggcacgatc gcgatggcgc gcacccccga
     2101 ccctgactcg gccgcggccc aattcttcat caacgtcaag gacaacgcct tcctcgatca
     2161 gtccaatgcc cgcgacggct acggctatgc cgtgttcggc aaggtgatca agggcatgga
     2221 cgtggtcgac aagatcaagc aggtccccac cggcaaccgc ggcgagcacc agaacgtgcc
     2281 gctgcagccg gtggtgatca ccaaagccac cgtggagaag tgaacatgag caagactatc
     2341 gaactgcaca ccaatctcgg cgtcatccgc gccgaactcg acgacgccaa ggcacccaag
     2401 accgtggcca acttccttgc ctatgcgggc aagggccatt acgacggcac ggtgttccac
     2461 cgagtgatca agggcttcat ggtgcaaggc ggcggcttcg agcccgggct caagcagaag
     2521 cccacggacg ccgcgatcga gaacgaggcc aacaacggcc tgaagaacga gaaatacacg
     2581 ctggccatgg cccgcaccag cgcgccccat tcggctaccg cccagttctt catcaacacc
     2641 gcgaacaacg agttcctgaa cttcaaatcc gagagctcgc aaggctgggg ttatgcggtg
     2701 ttcggcaagg ttgtggccgg tgccgacatc gtcgaccaga tcgagaaggt gcgcaccggc
     2761 cgcagcggct tccatgacga cgtgccgctc gaagacgtcg tgatcaccaa ggtcgtcgag
     2821 gtcgcctgag cttgatcacc ccggctgccg ccgcgccact gccgcatctt tcggactatt
     2881 ccgcgccggc aggctggcgc agcatcgact tcctgtccga cctgcacctg cagggctcga
     2941 cgccgcgcag cgtcgaagcc ctggcgcggc atctggagca gacgagcgcc gatgcggtct
     3001 tcatcctcgg tgatctgttc gaggcctggc ccggcgacga cgcccgcttc gacggcttcg
     3061 agcagcaatg cgcagcgatg ctgcgcgagg ccgctgcccg caagaccgtc ggcttcatgt
     3121 gcggcaatcg cgacttcctg gtcggcacag cgctgctgca ggactgcggc atgctcgccc
     3181 tgcccgaccc cacggtgctg atcgccttcg gacggcgcat cctgctgatc catggcgatg
     3241 cgctgtgcct ggccgatcac gactaccagc gcttccgcac ccaggtgcgc aacccggcct
     3301 ggcaggccgg cttcctggcc aagccgctga gcgagcggcg cgcgatcgcc cagcagatgc
     3361 gcgcccagag cgagcagcac aagcgcgagc agggtcgcga caactggatc gacctcgatc
     3421 gtgacgccac gctgcagtgg atggaggcgg ccgatacgcc gacgctgatc cacgggcata
     3481 cccaccatcc ctcgcacgag cggctggcac cgggccgcga gcgctgggtg ctgagcgact
     3541 gggacctcga ccacgaaccg cggcgcgccg atgtgttgag gctggatgcg gccggcatcc
     3601 ggcgcatcga tatcgccacg gcggcatgat cggctcctgg tggcgccggc ggcgcgaggc
     3661 gcgcgtgctg gcgcagcgcg ccatccccga gccgctgtgg cagaccacgc tggcgctcta
     3721 ccctttcctg cagctagcag accctgccga gcagcaggaa ctgcgacggc tgacaagcct
     3781 ctttcttgca cagaaggaat tcaccggcgc cggcggactg atcgtcaccg acgcgatggc
     3841 cgtggctgtc gccgcccagg cctgcctgcc catcctgcac ctggggctct caagctatgc
     3901 cggcttcgtc ggcatcgtga tccatcccga cgaggtcgtc gccaagcgcg agacgatgga
     3961 cgaggccggc gtcgtgcacc attacgacga agtgctggcg ggcgaggcga tggagggcgg
     4021 cccggtgatg ctgtcctggc gcgacgtggc cggtgccggc gagtcggcgg cccagggcta
     4081 caacgtcgtg atccacgaat tcgtccatgt gctggacatg gccgacggcc ggccgcccga
     4141 cggcataccg ccgctggcca gccgcgctgc gcgcgagcat tgggccatgg tgctggccgc
     4201 cgaatatgag cgcttctgcc acgcggtcga cgcgggctat gagaccttcc tcgatcccta
     4261 cggcacgacg ggcccggagg agttcttctc tgtcgcggcc gaagccttct tcgtcgcgcc
     4321 ggccgagctg cagcaagaac accgctacat gtacgagctg atggcgcact acttcaagca
     4381 ggacccggcg cagcgggcgg cccaaaaaga acggggagcc tgagcccccc gtcgcttgcg
     4441 ccgaacagaa aacagccggc cgtcagtccg tcgtcgcagc ttcggccttg ggtcgatcgc
     4501 tcttgcgggc cggctggatg tccagtactg gctgctcctt gtcgtcgata tccaccgtga
     4561 ggcggccgcc gtcgacaagg cgcccgaaca gcaactcgtc ggccagggcg cggcgtatcg
     4621 tgtcctggat cagccgctgc atcggccgcg cgcccatcaa cgggtcgaag cccttcttcg
     4681 ccagatgctt gcgcagcgca tcggtgaagg tgacctcgac cttcttctcg gccagctggc
     4741 tctcgagctg cagcaggaac ttgtcgacca cgcgcaggat gatctcctcg tccagcgaac
     4801 ggaagctcac gatcgcgtcg agccggttgc ggaactcggg cgcgaacagg cgcttgatgt
     4861 ccgccatctc gtcgccctgc tcgcgcgcgg tggtgaagcc gatggtcgcc ttgttcatcg
     4921 tctcggcgcc cgcgttggtg gtcatgatga tgatcacgtt gcggaagtcg gccttgcgcc
     4981 cgttgttgtc ggtcagcgtg ccatggtcca tcacctgcag cagcacgttg aagacatccg
     5041 gatgggcctt ctcgatctcg tcgagcagca gcaccgcatg cggcttcttg gtgatcgcct
     5101 cggtcagcag gccgccctga tcgaagccga catagcccgg gggcgcgccg atcaggcggc
     5161 tcaccgtgtg gcgctccatg tactcgctca tgtcgaagcg gatcagctcg atgcccagga
     5221 tgtaggcgag ctgtttggcc acctcggtct tgcccacgcc ggtggggccg ctgaacagga
     5281 aggagccgat cggcttgtcc ggcttgccca ggcccgagcg cgccatcttg atggccgcgg
     5341 cgagcgcatc gatcgccacg tcctggccga agaccacgct cttgaggtcg cgatccaggt
     5401 tcttgagctt ggagcgatcg tcgttgctga cggaggccgg cgggatgcgc gcgatcttcg
     5461 cgacgatctc ctcgacctcg gtgcgcgtga tggtcttctt ctgcttgttc ttgggcagga
     5521 tgcgctgggc cgcaccggct tcgtcgatca cgtcgatcgc cttgtcgggc agatggcggt
     5581 cgttgatgaa cttggccgac agctcggcgg ccgcctgcag cgcggtgacc gcgtacttga
     5641 cgctgtggtg ctcctcgaag cgcgacttca ggcccttgag gatctccacg gtctgctcga
     5701 ccgagggctc gaccacgtca atcttctgga agcgccgcga cagcgccgca tccttctcga
     5761 agatgcctcg gtattcgctg aaggtcgtgg cgcctatgca cttgagcgcc cccgtgctga
     5821 gcgccggctt gagcagattg ctcgcatcca gcgtgccgcc cgaggcggcg cctgcgccaa
     5881 tgagagtgtg gatctcgtcg atgaagagca ccgcgcccgg ctgctccttg agctgcttga
     5941 gcacgccctt caggcgctgc tcgaagtcgc cgcggtactt ggtgccggcc aagagcgcgc
     6001 ccatgtccag cgcatagacc gtcgcatcgg ccagcacttc tggcacctcg ccctgggtga
     6061 tgcgccaggc caggccctcg gcaatggccg tcttgcccac gccggcctcg ccgaccagca
     6121 gcgggttgtt cttgcggcgg cggcacagca cctggatcac gcgctcgacc tcgagctcgc
     6181 ggccgatcag cggatcgatc ttgccgtcct tggcgagctg gttgaggttc tgcgtgaact
     6241 gctccagcgg cgagcccttg gcttctccct cttccttctc gccttcgctg cccgcgccgt
     6301 cattggactt ggccggctcg ggcggctcgg acttcttgat gccatgggcg atgaagttca
     6361 ccacgtccag gcgcgtcacg ccctgctggt gcaggtagta gacggcatgc gagtccttct
     6421 cgccgaagat ggcgaccagg acgttcgcac cggtcacttc cttcttgccg ctgccggtgg
     6481 actgcacatg catgatggcg cgctggatga cgcgctggaa gcccagcgtc ggctgggtat
     6541 cgacatcgtc ggtgcccgcg acctgcggcg tgttgtcctt gatgaagttc gtgagcgacg
     6601 c
//
LOCUS       DASUCY010000185         4242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_68412, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000185 DASUCY010000000
VERSION     DASUCY010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68412"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="VFV25_03590"
     CDS             complement(<1..535)
                     /locus_tag="VFV25_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HEY1392428.1"
                     /translation="MVKKLLGLVFNRWVLLAALLLAVALVIWSIGPIVAIGESRPLES
                     ERSRWITILSMLGLTALVVAWQAWRARRGNNAVVQQLMTAPAAGDKKEAESPDMAAVR
                     ERFEKSLSTLRRARFSSGGGLLAGWSAKLGGRYLYELPWYLIIGAPGSGKTTALHNCG
                     LKFPLAETLGDHAVRGVG"
     gene            complement(550..1872)
                     /locus_tag="VFV25_03595"
     CDS             complement(550..1872)
                     /locus_tag="VFV25_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008346439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotU family type VI secretion system protein"
                     /protein_id="HEY1392429.1"
                     /translation="MDPASINDPFAQLDEQRTFMKPTPGGRAQRSVEPPIGEGAAPDI
                     ALPDEGLNPLVALANRLLLVVPQLRSTRRVDDPAALRNALAQGVRDFASQAAAQGIAP
                     ERVMAARYVLCTMLDEAAADTPWGGSGVWARHSLLAMFHNETWGGEKVFQLMARLAEK
                     PDANRDLLELIYCALTLGFEGRYRVIDNGHAQLEAVRDKLAQILKQQRGDYPHALAQH
                     WQTPPVQRARAYSWLPLAAAAALAALLLSGIYLALAISLGARSDPVYGQIQSLRLPPP
                     VVAQPQPAPKPRLAQFLQSDIKAGLVTVSDEIDRSVVTIRGDGSFATGSASLLPEREA
                     LMGRIADALSQVSGSVLVTGHTDNQPIARSARFPSNWHLSEERARTVRDLLVAHKVAA
                     ERIRVEGRADSEPLVPNDTPGNRALNRRVEVTLFVGRGADIAAKPANK"
     gene            complement(1900..3240)
                     /gene="tssK"
                     /locus_tag="VFV25_03600"
     CDS             complement(1900..3240)
                     /gene="tssK"
                     /locus_tag="VFV25_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HEY1392430.1"
                     /translation="MSLRSKVVWSQGMFLLPHHFQQEARHVDYALDMRLRACGPHAWG
                     HFELVLDEGLLSLGRLGLVRASGILPDGTPFSIPQNDAMPAPLDVPADVKGEIVYLAL
                     PLAREGVTEVDFDGGSEAGPELCRYRAIDGELRDQTNAGDEPEAVQTAALQLRLLRER
                     DAGDGFARLGVARVVERRSDNQVVLDRAYIAPQTRIEATAQLSATAGLLHGLMQQRAR
                     LLAGRMGQLSHGVSELADFMTLQALNRAEPLFRQHAGAPHTHPEIFHRDCLQLAGDLS
                     TLASESRHPAEYPLYKHDDLRATFASVVEDLRRMLSTVLERNALQIELVDRNYGVRTA
                     VIPDPELARNASFVLAVNAQMPAEQLRQRFPTQSKLGPVDRIRDLVNLQLPGIALRLL
                     PVAPRQLPFHAGFHYFELDRGGELWKQLERNGSLALHVAGDFPSLELELWAIRQ"
     gene            complement(3287..3814)
                     /gene="tssJ"
                     /locus_tag="VFV25_03605"
     CDS             complement(3287..3814)
                     /gene="tssJ"
                     /locus_tag="VFV25_03605"
                     /inference="COORDINATES: protein motif:HMM:TIGR03352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HEY1392431.1"
                     /translation="MSIFGVATRNWSSGQARLAPTRRPWFPAIAVSLAVVATLCGCAG
                     APKPAQIDATIQASAGVNPSPSKRPSPLLVRIYELKSAAAFNASDFVSLYQRDQAELA
                     GDLVGRDEFMLAPGESRSFAKTLGLETRYIGVIAAYRDLERSQWRSVVTIEPGKKQQL
                     LIRADALAISATVQK"
     gene            3972..>4242
                     /locus_tag="VFV25_03610"
     CDS             3972..>4242
                     /locus_tag="VFV25_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392432.1"
                     /translation="MRSLMLTLGCVAVALTTALPAQAQGLVGEFTHCIGRLRSELKRH
                     PEIRDETFETYASSAQDLRPLIDKATSTQPEFTQSVWDYLARRVDA"
BASE COUNT          653 a         1414 c         1514 g          661 t
ORIGIN      
        1 cgcccacgcc gcgcaccgca tggtcgccca gggtctcggc gagcggaaac ttcaggccgc
       61 agttgtgcag ggccgtggtc ttgcccgatc caggcgcgcc gatgatgagg taccagggca
      121 gctcataaag atatcgcccg ccgagcttgg ccgaccagcc cgcgagtagc ccgccgccgc
      181 tgctgaagcg cgctcgcctg agcgtcgaga gcgacttctc gaagcgctcg cgcaccgcgg
      241 ccatgtcggg gctttccgct tccttcttgt cgccggcggc gggcgcggtc atcagctgct
      301 gcacgacggc gttgttgccg cgccgtgcgc gccaggcttg ccaggcgacg accagcgccg
      361 tgagacccag catcgagagg atggtgatcc agcgcgagcg ctcgctttcc agcgggcgcg
      421 attcgccgat cgcgacgata gggccgatgc tccagatcac cagcgccacg gcgagcagca
      481 gcgccgccag cagcacccag cggttgaaga cgaggccgag cagcttcttg accatcgccg
      541 tctcgttgcc tacttgttcg ccggcttggc ggcaatgtcg gcgccacggc cgacgaagag
      601 cgtgacctcg acgcggcggt tcagcgcgcg gttgccgggc gtgtcgttgg gcaccagcgg
      661 ctcgctgtcg gcgcgtccct cgacgcgtat gcgctcggcg gccaccttgt gcgcgacgag
      721 aaggtcgcgc acggtgcgcg ccctttcctc ggacagatgc cagttggagg ggaagcgcgc
      781 cgagcgcgcg atcggctggt tgtcggtgtg gccggtcacc aggacggagc cgctgacctg
      841 cgacagcgca tcggcgatgc ggcccatcaa ggcctcgcgc tcgggcagca ggctggcgct
      901 gccggtggcg aaggagccgt cgccgcgtat cgtcacgacg ctgcggtcga tttcgtcgct
      961 caccgtcacg agccccgctt tgatgtcgga ctgcaggaac tgcgcgagcc gcggcttggg
     1021 cgcgggctgc ggctgcgcca ccacgggcgg cggcagtcgc aggctttgga tctggccgta
     1081 gaccgggtcg gaacgagcgc ccagcgagat ggccagcgcc aggtagatgc cgctgagcag
     1141 cagcgcggcc agcgcggcgg cggcggccag cggcagccag ctataggcgc gggcgcgctg
     1201 caccggcggg gtctgccaat gctgggccag cgcatgcggg tagtcgccgc gctgctgctt
     1261 gaggatctgc gccagcttgt cgcgcaccgc ttcgagctgg gcgtgaccgt tgtcgatgac
     1321 gcggtagcgg ccctcgaagc ccagcgtcag cgcgcagtag atcagctcca gcaggtcgcg
     1381 attggcatcg ggcttctcgg ccaggcgagc catcagctgg aacaccttct cgccgcccca
     1441 ggtctcgttg tggaacatcg caagcaggct gtgcctcgcc cacacgcccg agccgcccca
     1501 gggcgtgtcg gcggcggcct cgtcgagcat ggtgcacagc acatagcgcg ccgccatcac
     1561 gcgttccggc gcgatgccct gcgcggcggc ctggctggcg aaatcgcgca cgccctgggc
     1621 gagcgcattg cgcagggccg cggggtcgtc gacgcgccgc gtgctgcgca gctgcggcac
     1681 caccagcagc aggcggttgg ccagcgcaac cagcgggttg agcccttcat cgggcagtgc
     1741 gatatcggga gcggcgccct cgccgatcgg aggctccacg ctgcgctgtg cccgcccgcc
     1801 aggcgtgggc ttcatgaagg tgcgctgctc gtcgagctga gcgaaggggt cgttgatcga
     1861 ggcggggtcc atagggctcg ctgcgctttc agcgccccgt cattggcgta tcgcccacag
     1921 ctcgagttcg aggctgggga agtcgccggc cacgtgcagg gccaggctgc cgttgcgctc
     1981 cagctgcttc cacagctcgc cgccgcggtc cagctcgaaa tagtggaagc cggcatggaa
     2041 gggcagctgg cgcggtgcca ccggcagcag gcgcagcgca atgccgggca attgcaggtt
     2101 cactaggtcg cggatgcggt ccaccgggcc gagcttcgac tgcgtcggga agcgctggcg
     2161 cagctgctcg gccggcatct gcgcattgac cgccagcacg aagctggcat tgcgcgccag
     2221 ctcggggtcg gggatgaccg ctgtgcgcac gccatagttg cgatcgacca gctcgatctg
     2281 cagcgcattg cgctccagca ccgttgacag catgcgccgc aggtcttcca ctaccgacgc
     2341 gaaggtggcg cgcagatcgt cgtgcttgta cagaggatat tcggcgggat gccgcgactc
     2401 gctggccaga gtcgaaagat cacccgccag ctgcaggcaa tcgcgatgga agatctcggg
     2461 gtgggtgtga ggcgcgcccg cgtgctggcg aaacagcggc tcggcgcggt tgagcgcctg
     2521 cagcgtcatg aagtcggcca gctccgagac accgtggctc agctggccca tgcggccggc
     2581 cagcaggcgc gcgcgctgct gcatcagtcc atgcaggagg ccggcggtgg ccgacagctg
     2641 tgccgtggcc tcgatgcgcg tctgcggcgc gatataggcg cggtccaaaa cgacctggtt
     2701 gtcgctgcgg cgctcgacca ccctggccac gcccagccgc gcgaagccgt cgccggcgtc
     2761 gcgctcgcgc agcaggcgca gctgcagagc cgcggtctgc accgcctcgg gctcgtcacc
     2821 ggcattggtc tggtcgcgca gctcgccgtc gatggcgcgg tagcggcaca gttcggggcc
     2881 ggcctccgag ccgccgtcga agtcgacttc ggtcacgcct tcgcgcgcaa gcgggagggc
     2941 caggtagacg atctcgccct tcacgtcggc cggcacgtcc agcggcgcgg gcatggcatc
     3001 gttctgcggg atggaaaagg gcgtgccgtc gggcaggatg ccgctggcgc gcaccaggcc
     3061 caggcggccg agcgacaaca ggccctcgtc cagcaccagc tcgaaatggc cccaggcatg
     3121 cggcccgcag gcgcgcaagc gcatgtccag cgcatagtcg acgtggcgcg cttcctgctg
     3181 gaagtgatgc ggcaacagga acatgccctg cgaccacacg accttgctgc gcaaactcac
     3241 ggggtctccg gggcgggttg ctgtctacgg ccgggtgttc ttgcagtcac ttctgaaccg
     3301 tcgccgagat cgccaaggcg tcggcgcgga tcagcaactg ctgcttcttg ccgggctcta
     3361 tcgtgacgac gctgcgccat tgcgagcgct cgaggtcgcg gtaggccgcg atcacgccga
     3421 tatagcgggt ctccagcccc agcgtcttgg cgaaggagcg cgattcgccg ggcgcgagca
     3481 tgaattcgtc gcgccccacc aggtctcccg ccagttcggc ctggtcgcgc tgatagagcg
     3541 acacgaaatc actggcgttg aaggcggcag ccgacttgag ctcgtagatg cgcaccagga
     3601 gcggcgaggg ccgcttgctg gggctggggt tgacgccggc agaggcctga atggttgcgt
     3661 cgatctgcgc cggcttgggc gcgccggcgc atccgcagag cgtcgcgacg acggccaggc
     3721 tgactgcgat ggctgggaac cacgggcggc gagttggagc gagccttgct tgcccggatg
     3781 accagttccg cgtcgccacg ccgaagatgc tcacgggagt ccactcctgt tgtcgctcga
     3841 agaatagcca gagcagccgc tggggcctcg tgacttaggt aatccatgac ggggtcatgt
     3901 gtcgccaatg gtgtccgctc taaactgcac ccgatcccgt tctgcgcaag caaaaaagga
     3961 gcgctcatcg gatgcgaagc ttgatgctca cgctaggctg tgtggcggtt gccttgacga
     4021 cggcgctgcc ggcgcaggcc caggggctgg tgggtgaatt cacgcactgc ataggcaggc
     4081 tcaggagcga gctcaagcgg caccctgaga tccgcgacga gaccttcgag acctatgcga
     4141 gcagtgccca ggacctgcgc ccgctgatcg acaaggccac cagcacgcaa cccgagttca
     4201 cgcagtcggt gtgggactat ctggcgcgcc gcgtcgatgc cc
//
LOCUS       DASUCY010000186         2200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_662747, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000186 DASUCY010000000
VERSION     DASUCY010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662747"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VFV25_03615"
     CDS             complement(<1..497)
                     /locus_tag="VFV25_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392433.1"
                     /translation="MFKFHLQAPRLALACALLAAGTAHAFDFSTINLLSQGEFRELSE
                     DVAAVSSYKPLAPAEATGLAGFDVGLSASGTGLQHRDVWRKVTVNGDVPGTLPVVGLR
                     AQKGLPLNIDVGLSYSMVPTTSVRATGGELRWAVLPGSALTPAIAVRLSASSLSGVDQ
                     LKLRTT"
     gene            789..>2200
                     /locus_tag="VFV25_03620"
     CDS             789..>2200
                     /locus_tag="VFV25_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY1392434.1"
                     /translation="MATRSTTSSSSAGSYGEASIRVLKGLEPVKQRPGMYTRTDNPLH
                     IVQEVIDNAADEALAGYGKRIAVTLHADGSVSVDDDGRGIPFGLHPEEKVPVVEIVFT
                     RLHAGGKFDKGSGGAYSFSGGLHGVGVSVTNALAKRLQVTVFRDAQVAQLAFSGGDVI
                     EPLAKRKAAADDRRQGTSVRVWPDAKYFESAELPRQELIHLLRSKAVLMPGVTVTLTH
                     EKTGETQTWAYKGGLRDYLMQTLQADPVIPLFEGEHYAEEGETENFAEGEGAAWVLAF
                     TEEGNATRESYVNLIPTVAGGTHEAGLKDGLFGAVKGFIDMHSLLPKGVKLMPDDVFS
                     RASFVLSAKVLDPQFQGQTKERLNSRDALRLVSTYVKPALELWLNQHVEHGKKLAELV
                     IKQAQARQRAGQKVEKRKGSGVAVLPGKLTDCESRDTSLNELFLVEGDSAGGSAKMGR
                     DKETQAILPLRGKVLNAWEV"
BASE COUNT          397 a          706 c          774 g          323 t
ORIGIN      
        1 gtggtgcgca gcttcaactg gtccacgccg gacaagctcg aggccgagag cctgactgca
       61 atcgccggcg ttagcgcgct gccgggcagc accgcccagc gcagctcgcc gccggtggcg
      121 cgcacgctgg tggtcgggac catgctgtac gagaggccga cgtcgatatt gagcggcagc
      181 cccttctggg cgcgcaggcc gaccacgggc agcgtgccgg gcacatcgcc gttgaccgtc
      241 accttgcgcc acacgtcgcg gtgctgcagc ccggtgccgc tggcgctgag gccgacatcg
      301 aagccggcga ggccggtcgc ctcggccggc gcgagcggct tgtaggacga aacggcggcg
      361 acgtcctcgc tgagctcgcg gaattcgccc tggctcagca gattgatggt gctgaaatcg
      421 aaggcatggg cagtgccggc ggccagcagg gcgcacgcca atgcaaggcg cggggcttga
      481 aggtggaact tgaacatggg aggtgttggg atggggagct gaaaccggcg cgaggctagc
      541 actggcgcgg cggcgctttg ctatacaaaa ggtgggcttg tcacctcttc acacgatttg
      601 gtgcgccgcg ccgatgggct gtggcgcgga gcctacaggc ggggccgtcg gccagcggcg
      661 ggtcaggtga cggcaggaag cgagaacttt tctgatagtt acccgaatta gattgagaca
      721 ttaaagtacg tattttgtag cagctgggtt ccaaggccag ccaccgtgcc gccctagaat
      781 cgccgcccat ggcgacccga tccacgactt cctccagcag cgccggcagc tacggcgaag
      841 cctcgatccg cgtgcttaag ggcctggagc ccgtcaagca gcggccgggc atgtacaccc
      901 gcaccgacaa cccgctgcac atcgtccagg aggtgattga caacgccgcc gacgaggccc
      961 tggcaggcta cggcaagcgc atcgccgtga cgctgcatgc cgatggctcg gtcagcgtgg
     1021 acgacgatgg ccgcggcatc cccttcggcc tgcacccgga agagaaggtg ccggtggtcg
     1081 agattgtctt cacccggctg cacgccggcg gcaagttcga caagggctcg ggcggcgcct
     1141 acagcttctc gggcggcttg cacggcgtgg gcgtgtcggt caccaacgcg ctggccaagc
     1201 ggctgcaggt cacggtgttc cgcgacgctc aggtggcgca gctcgccttc agcggcggcg
     1261 acgtgatcga gccactggcc aagcgcaagg ccgcggccga tgatcgtcgc caaggcacca
     1321 gcgtgcgcgt ctggcccgac gccaagtatt tcgagagtgc cgagctgccg cggcaagagc
     1381 tgatccatct gctgcgcagc aaagccgtgc tgatgcccgg cgtcacggtg acgctgactc
     1441 acgagaaaac gggcgagacg caaacctggg cctacaaggg cggcctgcgc gactacctga
     1501 tgcagaccct tcaggccgac ccggtgatcc cgctcttcga gggcgagcac tacgccgagg
     1561 agggcgagac cgagaacttc gccgaaggcg agggcgctgc ttgggtgctg gccttcaccg
     1621 aagagggcaa cgccacgcgc gagagctatg tcaacctcat ccccacggtg gccggcggca
     1681 cgcacgaggc ggggctgaag gacggcctct tcggcgcggt gaagggcttc atcgacatgc
     1741 acagcctctt gcccaagggc gtgaagctga tgcccgacga cgtgttctcg cgcgcgagct
     1801 tcgtgctctc ggccaaggtg ttggacccgc aattccaggg ccagaccaag gagcggctca
     1861 acagccgcga cgcgctgcgg ctggtgtcca cctatgtgaa gccggcgctg gagctatggc
     1921 tcaaccagca tgtggagcac ggcaagaagc tggccgagct ggtcatcaag caggcccagg
     1981 cccgccagcg cgccggccag aaggtggaaa agcgcaaggg ctccggcgtg gccgtgctgc
     2041 ccggcaagct caccgactgc gaaagccgcg acacctcgct caacgagctc ttcctggtcg
     2101 agggcgattc cgccggcggc agcgccaaga tgggccgcga caaggagacg caggccatcc
     2161 tgccgctgcg cggcaaggtg ctcaacgcct gggaggtgga
//
LOCUS       DASUCY010000187         3003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_855501, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000187 DASUCY010000000
VERSION     DASUCY010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855501"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VFV25_03625"
     CDS             complement(<1..237)
                     /locus_tag="VFV25_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392435.1"
                     /translation="MFQKTKVCKGLMLAFGGSLALSALPALAQQTQDQQQPQQLQRVE
                     ITGSAIKRIDAETAVPVTVIKMDDLKKTGVTSVEQ"
     gene            complement(594..1502)
                     /gene="rsgA"
                     /locus_tag="VFV25_03630"
     CDS             complement(594..1502)
                     /gene="rsgA"
                     /locus_tag="VFV25_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEY1392436.1"
                     /translation="MTKAVVLDSGLVVASHGRHYIVETPEGERILCHPRGKRSDAVVG
                     DRVRWQAAGDEGVIEHIEPRRNLLFRQDEWRTKSFAANLDQLLVWVAAEPVFSESQLT
                     RALIAAEAAGIAAHIVLNKTDLPGAGAARERLASYREMGYELVELSLKGDAPAARARL
                     APLLQERSTLVLGPSGAGKSTLINLMIPNAKAQVGEISQALNTGRHTTTTTQWYWLDA
                     AERRTALIDSPGFQEFGLRQIEAAQLPGLMPDFRAYEGQCRFYNCTHLHEPGCAARAA
                     LEAGRISRSRYAIYESLRAELSAPRI"
     gene            complement(1513..1836)
                     /locus_tag="VFV25_03635"
     CDS             complement(1513..1836)
                     /locus_tag="VFV25_03635"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008111729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEY1392437.1"
                     /translation="MSKTDLTRMKCAPQNTAMGEADIAARLADIPGWTLKDGAIEKTW
                     RFKNYHETMAFVNALAWIAHREDHHPDLQVGYNHCTVRYSTHSVGGLSINDFICAAKI
                     DALIA"
     gene            complement(1833..>3003)
                     /locus_tag="VFV25_03640"
     CDS             complement(1833..>3003)
                     /locus_tag="VFV25_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEY1392438.1"
                     /translation="VPAAFAATVSLAAHQRAADYTIAKLRFGLATLAFGTVVLLGWTL
                     LGGLDALNHVVRAAVQPLFAGGWGDLAYQLALLAAFAVIGGLLDLPFEAWSTFRIEQR
                     YGFNRMSWRLWLADLFKGLLLGVVIGAPIAALILWLMGAAGAWWWLWAWGVWMGFNLL
                     MLVLYPTVIAPLFNKFQPLQDEALKARVEALMARCGFAAKGLFVMDGSKRSAHGNAYF
                     TGFGAAKRVVFFDTLLNKLQPGEVEAVLAHELGHFKHRHVLKRIVAMFALSLAGFWLL
                     GWLMQQVWFYTGLGVQPALDTPNNALALLLFMLAGPVFVYFVSPVFAGLSRRHEFEAD
                     AYACAQASGKDLASALLKLYEDNASTLTPDRLYVRFYYSHPPASERLAAMGAAAT"
BASE COUNT          516 a          955 c         1007 g          525 t
ORIGIN      
        1 ttgctcgacc gaggtcacac cggtcttctt caggtcatcc atcttgatga ccgtgacggg
       61 cacggcggtc tcggcgtcga tgcgcttgat ggccgagccg gtaatctcga cgcgctgcag
      121 ctgttgcggt tgctgctgat cttgcgtctg ctgggccagc gccggtaggg cgcttagagc
      181 gagcgacccg ccaaaggcga gcatcaggcc cttgcagacc ttggtctttt ggaacatgtg
      241 gactcctggg taatcccgaa agttgatcga tagaccctgt ctgatagcaa gccgtgtacc
      301 gggtttgtga tccggtcatg actcgtctgc caagacatgt aaatgaattg tcacgcgggg
      361 caatagcgcg gcggcttagg tgaattccgc ggcgcagcga atgctgacaa aacgctgact
      421 aattgcgact ccgctgtttc aagcgaatgc ccggagagcc gcgaatttat tggctttgcg
      481 agacggcggg gtcattcgtg attgctgcga acgatgtccc cgcacgttgg ctgttcgcaa
      541 cagcaaatcc cctgtggcag agaactgttt ggtctactgc ccctcgtcct tcctcaaata
      601 cgaggcgccg acagttccgc ccgcaaactt tcgtagatcg cgtagcggct gcggctgatg
      661 cgccccgcct cgagggcagc ccgtgccgcg cagccgggct catgcagatg ggtgcagttg
      721 tagaagcggc actggccttc gtaggccctg aagtcgggca tcaggcctgg aagttgtgcg
      781 gcctcgatct ggcgcaggcc gaattcctga aagccgggcg agtcgatcag ggcggtccgg
      841 cgctctgcgg cgtccagcca gtaccactgt gtcgtcgtcg tcgtgtggcg tccggtattc
      901 agggcctgcg atatctcgcc gacctgggct ttcgcgttcg ggatcatcag gttgatcagc
      961 gtgctcttac cggctccgga aggccccagc accaacgtgc tgcgttcttg cagcagcggc
     1021 gccagccgcg ctcttgctgc cggcgcatcg cccttgaggc tcagctcgac cagttcgtac
     1081 cccatctccc gataggacgc gaggcgttcg cgggccgcgc ccgctcccgg caggtcggtc
     1141 ttgttcagca cgatgtgcgc ggcgatgccg gctgcctcgg ccgcgatcag cgcgcgggtg
     1201 agctgggatt cgctgaacac cggctccgcc gcgacccaga ccagcagctg gtccaggttg
     1261 gccgcgaagg acttggtgcg ccattcgtcc tggcggaaca gcaggttgcg gcgcggctcg
     1321 atgtgctcga tcacgccctc gtcgccggcg gcctgccagc gcacgcggtc accgacgacc
     1381 gcatcgctgc gcttgccgcg ggggtggcag aggatgcgct cgccttcggg cgtctcgacg
     1441 atgtagtggc ggccatggct ggccaccaca aggccgctgt cgaggacgac ggccttggtc
     1501 aaacgccggc cctcatgcga tcagcgcgtc gatcttggcc gcgcagatga agtcgttgat
     1561 cgacaacccg cccaccgaat gcgtgctgta gcgcacggtg caatggttgt aacccacttg
     1621 caggtcggga tggtggtctt cgcggtgcgc gatccaggcc agcgcgttca cgaaggccat
     1681 ggtctcgtgg tagttcttga agcgccaggt cttctcgatc gcgccgtcct tgagcgtcca
     1741 gcctgggatg tcggcgaggc gggcagcaat gtcggcctcg cccatcgccg tgttctgcgg
     1801 cgcgcacttc atccgtgtca gatcggtctt gctcacgttg ctgcggcccc catcgcggca
     1861 agccgctccg aagcgggcgg gtgcgaataa tagaagcgca cgtagagccg atccggcgtc
     1921 agcgtcgagg cgttgtcctc gtagagcttg agcaaggcgc tggccaggtc tttgccgctg
     1981 gcttgcgcgc aggcataggc atcggcctcg aactcatggc ggcgcgacag gccggcaaag
     2041 acgggcgaca cgaagtagac gaacaccggc ccggccagca tgaagagcag cagggccagc
     2101 gcgttgttgg gcgtgtccag ggccggctgc acgcccaggc cggtgtagaa ccacacttgc
     2161 tgcatcagcc agcccagcag ccagaagccg gccaggctga gcgcaaacat cgcgacgatg
     2221 cgcttgagca cgtggcggtg cttgaaatgg cccagttcat gcgccagcac ggcttccacc
     2281 tcgccgggct gcagcttgtt cagcagcgtg tcgaagaaga ccacgcgctt ggccgcgccg
     2341 aagccggtga aataggcatt gccatgcgcc gagcgcttgc tgccgtccat cacgaacaag
     2401 cccttggccg cgaagccgca gcgcgccatc agcgcctcga cgcgggcttt caaggcctcg
     2461 tcctgcagcg gttggaattt gttgaagagc ggcgcgatga cggtggggta gagcacaagc
     2521 atcaggaggt tgaagcccat ccacacgccc caggcccaca gccaccacca ggcgccggcc
     2581 gcccccatca gccacaggat cagcgcggcg atgggtgcgc cgatcacgac gccgagcagc
     2641 aagcccttga agagatcggc caaccacagt cgccagctca tgcggttgaa gccgtagcgc
     2701 tgctcgatgc ggaaggtgct ccaggcttcg aagggcaggt cgagcaggcc gccgatgacg
     2761 gcgaaggccg ccagcagcgc gagctgatag gccaggtcgc cccaacctcc agcaaagagg
     2821 ggctgcacgg cggcgcgcac cacgtggttg agcgcgtcca gcccgccgag cagcgtccag
     2881 cccagcagca ccacggtgcc gaaggcgagg gtcgcgaggc cgaagcgcag cttggcgatg
     2941 gtgtagtcgg ccgcgcgctg gtgggcggcc agcgagacgg tggcggcaaa ggccgccggc
     3001 act
//
LOCUS       DASUCY010000188         3875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_582379, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000188 DASUCY010000000
VERSION     DASUCY010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582379"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /gene="mutY"
                     /locus_tag="VFV25_03645"
     CDS             complement(<1..679)
                     /gene="mutY"
                     /locus_tag="VFV25_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEY1392439.1"
                     /translation="MELPEDFAARLVAWQRIHGRHDLPWQRTREPYRVWLSEIMLQQT
                     QVSTVLAYYPRFLQRFPDVRSLAAASLDEVLAAWAGLGYYSRARNLHRCAQVVLEQHG
                     GEFPRSAAALVELPGIGRSTAAAIAAFCGDERAAILDGNVKRVLSRLLAFGEDLAQAR
                     NEKTLWACAEALLPAAAGDMPAYTQGLMDLGATICLARKPACALCPTQGLCAAQREGR
                     PEAYPVKT"
     gene            complement(721..2802)
                     /locus_tag="VFV25_03650"
     CDS             complement(721..2802)
                     /locus_tag="VFV25_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HEY1392440.1"
                     /translation="MQQQQSFARSLDALGSWRAALEARLKELGRFLSEHELLDEPAAD
                     TLDALRQRLAGDKLVVAFVAEFSRGKSELVNAIFFADAGRRILPATPGRTTMCPVELG
                     YDPDEPPGLALLPIETRLESLSLSELRGQPKAWKLMPLNLADADQLAQTLTEVMRTKA
                     VSKDDARALGFWDDERPEENPPADSHGRVEVPAWRHALINVPHPLLKRGLVVLDTPGL
                     NAIGAEPELTVGLLPSAHATVFILSADAGVTKSDLAIWRDHLSSHALARYVALNKIDA
                     LVDPLSPKAHTDAHIARQCEEVARTLDIEPQRVFPISARQALAARVAKDEASLAESRL
                     PALENALAEELLPQRRELLARVTLEGARQIQTQLTRRLNDRRRQLAEQMLELRGLRGK
                     NSGKIRMMKSRVDAEIAEFEQCQARLQAMRSVHTRMLKDTLVGLSSDRVREQVNAMQE
                     AVRGSLLQLGAKKAFVEMCGALRGLVEQAQARAGEIHDMLAASFAKLNAEFGFGLAVA
                     QGPDLSRFVGDLELIERNYVQYLGLTQALRLSQPRFMEQFRRMLVSRLRVVFESACGE
                     LELWNKTASAQIDSQLRERRRAFKRRRESMDRIEQAAGDLEGRLTELEAQDQRLQQLQ
                     TRFKLQLDGLATAAGIKPPAADAEPDREPRAAAEETTPLKIKLIDLPEETGGVDVLLD
                     LEDTTPRLVRA"
     gene            complement(2948..3778)
                     /gene="mutM"
                     /locus_tag="VFV25_03655"
     CDS             complement(2948..3778)
                     /gene="mutM"
                     /locus_tag="VFV25_03655"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEY1392441.1"
                     /translation="MPELPEVEVTRLSFAERIRGARVIAVRLGKPLRWPLGCRPEALH
                     DQIVGEVTRRGKYLWLPLVAETPAHGGLLLHLGMSGSLSFAPALPAPGPHDHFELHTT
                     HGLLRLTDPRRFGAVLWARSQRSGMAAKLLAPLGLEPFDPGFDASHLHARLRRRRVAI
                     KQALLAGDIVVGVGNIYVSEALFRAGIDPRARSDRISLARCEKLVEAVRDTLGRALEL
                     GGSTLRNFRDAHGMGGAFQLQAGVYDREGLPCPVCGTAVRRIMQGQRSSYFCPSCQRR
                     "
BASE COUNT          593 a         1302 c         1387 g          593 t
ORIGIN      
        1 gcgtcttcac cggataggcc tcgggccggc cctcgcgttg cgcggcgcac aggccttgag
       61 tcgggcacag cgcgcaggct ggcttgcgtg ccaggcagat cgtcgcgccc aggtccatca
      121 ggccctgcgt ataggcaggc atgtcgccgg cggcggccgg cagcagagcc tcggcacagg
      181 cccacagcgt cttctcgttg cgggcctgcg cgaggtcttc gccgaaggcg agtagccggc
      241 tcagcacacg cttgacattg ccgtcgagga tcgcagctcg ttcgtcgcca cagaaggcag
      301 cgatcgccgc ggcggtcgag cggccgatgc cgggcagctc gaccagcgcc gcagcgctgc
      361 gcggaaactc gccgccatgc tgttccagca cgacttgcgc gcagcggtga agattgcgcg
      421 cgcggctgta gtagcccagc cccgcccaag cggcgagcac ttcgtccagc gacgctgcgg
      481 cgagcgagcg cacgtcgggg aagcgctgca ggaagcgtgg gtagtaggcg agcactgtgc
      541 tcacctgggt ctgctgcagc atgatctcgg acagccacac gcgatagggc tcgcgcgtgc
      601 gctgccaggg cagatcgtgg cggccgtgta ttcgctgcca ggcgaccagg cgcgcggcga
      661 agtcctcggg cagctccatg gcagcggccg gcgccgccgc gcgttcggaa gaacgagcca
      721 tcaggcccgc acgagacgag gcgtggtgtc ttcgagatcg agcagcacgt cgacgccgcc
      781 cgtctcctcg ggcaggtcga tcagcttgat cttgagcggc gtggtctctt ctgctgcggc
      841 ccgcggctcg cgatcgggtt ccgcatctgc agccgggggc ttgatgcccg ccgcagtggc
      901 caggccgtcg agctgcagct tgaagcgggt ctgcagctgc tgcaggcgct ggtcctgcgc
      961 ctccagttcg gtcaggcggc cttccagatc gccggcagcc tgctcgatgc ggtccatgga
     1021 ctcgcggcgg cgcttgaagg cgcggcggcg ttcgcgcagc tgcgagtcga tctgggccga
     1081 ggcggtcttg ttccagagct cgagctcgcc gcaggcggac tcgaagacca cgcgcagccg
     1141 cgacaccagc atgcggcgga actgctccat gaagcgcggc tgcgacaggc gcagcgcctg
     1201 ggtcagcccc aggtactgga cgtagttgcg ctcgatcagc tccaggtcgc cgacgaagcg
     1261 gctcaggtcc ggcccctggg ccacggcgag cccgaagccg aactcggcgt tcagcttggc
     1321 gaaggaggcg gccagcatgt cgtgaatctc gccggcccgg gcctgggctt gctcgaccag
     1381 gccgcgtaaa gcgccgcaca tctcgacgaa ggccttcttg gcgcccagct gcagcagcga
     1441 gccgcgcacg gcttcctgca tcgcattgac ctgctcgcgc acgcggtcgc tggacaggcc
     1501 gaccagggtg tccttcaaca tgcgggtgtg caccgagcgc atggcctgaa gtcgcgcctg
     1561 gcattgctcg aactcggcga tctcggcgtc gacgcgcgac ttcatcatgc ggatcttgcc
     1621 gctgttcttg ccgcgcaggc cgcgcagctc cagcatctgc tcggcgagct ggcggcggcg
     1681 gtcgttgagg cggcgcgtga gctgggtctg gatctgccgc gccccttcga gcgtcactcg
     1741 cgccagcagc tcgcggcgct gcggcagcag ctcttcggcc agcgcgttct ccagcgccgg
     1801 caggcggctc tcggcgaggc tggcctcgtc cttggccacg cgcgcggcca gggcttggcg
     1861 cgccgagatc gggaacacgc gctgcggctc gatgtccagc gtgcgggcca cttcctcgca
     1921 ttgccgcgcg atatgggcat cggtatgcgc cttggggctc aggggatcga ccagcgcgtc
     1981 gatcttgttc agcgccacat agcgcgccag cgcatggctg ctgagatggt cgcgccagat
     2041 cgcgaggtcg gacttggtca cgccggcatc ggcgctcagg atgaagacgg tggcatgggc
     2101 gctgggcagc aggcccacgg tgagctcggg ctccgcgcct atcgcgttga ggccgggcgt
     2161 gtccagcacc accaggccgc gcttgagcag cgggtgaggc acgttgatca gcgcatggcg
     2221 ccaggccggc acctcgacgc ggccatggga gtcggccgga gggttttcct cggggcgctc
     2281 gtcgtcccag aagcccagcg cgcgcgcgtc gtccttggac acggccttgg tgcgcatcac
     2341 ttcggtgaga gtctgcgcga gctggtcggc atcggccaga ttcagcggca tcagcttcca
     2401 ggccttgggc tggccgcgca gctcgctcag cgacaggctt tccagccgcg tctctatggg
     2461 cagcagcgcg aggccgggcg gctcgtcggg gtcgtagccc agctccaccg ggcacatcgt
     2521 cgtgcgcccc ggcgtggcgg gcaggatgcg ccggccggca tcggcgaaga agatcgcatt
     2581 gaccagctcg gacttgccgc gcgagaactc ggcaacgaag gccaccacca gcttgtcgcc
     2641 ggccaggcgc tggcgcagcg catcgagcgt gtccgcggcc ggctcgtcga gcagctcatg
     2701 ctcgctcagg aagcgcccca gctccttgag gcgagcttcc agcgccgcgc gccaactgcc
     2761 aagggcatcg agactgcggg cgaaggattg ctgctgctgc atggggctgc aagaaaggaa
     2821 aaaagacgca aattaacccg ggccatggta gcgcaagcac ccatgcgccc caatcgggct
     2881 gttgcgcagc gcatcacgct ccaggccccg gccttgtgtg cgtttgttac ggcaggcggc
     2941 ggccgcctca gcgccgctgg cagctggggc agaagtagct ggagcgctgg ccctgcatga
     3001 tgcggcgcac cgcggtgccg cagacggggc agggcaggcc ttcgcggtcg tagacaccgg
     3061 cctgcagctg aaaggcgccg cccatgccgt gggcatcgcg gaaattgcgc agcgttgagc
     3121 cgcccagctc cagcgcgcgg cccagcgtgt cgcgcacggc ctcgaccagc ttctcgcagc
     3181 gcgcgaggct gatacggtcg ctgcgcgctc gagggtcgat gccggcccgg aacagcgctt
     3241 ccgagacata gatgttgccc acgccgacca cgatgtcgcc ggccagcagg gcttgcttga
     3301 tcgccacgcg gcgacggcgc aggcgggcat gcaggtggct cgcgtcgaag ccgggatcga
     3361 aaggctccag ccccagcggc gcgagcagct tggctgccat gcctgagcgc tgcgaacgcg
     3421 cccacagcac ggcgccgaag cggcgcggat ctgtcaggcg caagaggcca tgcgtggtgt
     3481 gcagctcgaa atgatcgtgc gggcccggtg ccggcagggc cggtgcgaag ctcagcgagc
     3541 ccgacatgcc cagatgcagc agcaggccgc cgtgagcggg cgtttcggcc accagcggga
     3601 gccacaggta tttgccgcgc cgcgtcacct cgccgacgat ctggtcgtgc aaggcctcgg
     3661 gcctgcagcc cagaggccag cgcagcggct tgcccagacg caccgcgatc acgcgtgcgc
     3721 cgcgtatgcg ctcggcgaag ctcaggcgcg tgacttcgac ttccggcagc tcgggcatgg
     3781 gaccattatg tggcgctgga gagcgtgcaa agattggcaa agtttctgat gattgcccgt
     3841 caaattttgc cgatgacaag tacgaaaaaa gtagc
//
LOCUS       DASUCY010000189         2284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_389593, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000189 DASUCY010000000
VERSION     DASUCY010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389593"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="VFV25_03660"
     CDS             <1..788
                     /locus_tag="VFV25_03660"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEY1392442.1"
                     /translation="QFQAAGGPGFVLRELLDGGWLHEDVATVSRQGLRDYTRVPALEG
                     GALQWRELPDASADESIVRCGAAPFSATGGLKLLTGNLGRSVIKVSAVPEDRHVIEAP
                     ALVFDSQEALLAAFKGGSLERDFVAVVRYQGPQANGMPELHKLTPPLAVLQGKGFRVA
                     LVTDGRMSGASGKVPAAIHLSPEAMDGGPLGKVRDGDVIRLDAVAGTLQALVPEAEWA
                     SREAVAPSADYLGEHAHGLGRELFAGLRRNVRAAEQGACTWLG"
     gene            806..1429
                     /gene="eda"
                     /locus_tag="VFV25_03665"
     CDS             806..1429
                     /gene="eda"
                     /locus_tag="VFV25_03665"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEY1392443.1"
                     /translation="MNTLALASHGPVIPVIVIKRLQDAVPLARALLAGGVRVLEVTLR
                     TPVALQAMEAIARELPEAIVGAGTVRSVADAKAALAAGCRFAVSPGYASDIGRACRDI
                     GLPLLPGVATASEVMMANADGYHFLKFFPATAAGGIPMLKALAGPFPDVAFCPTGGIT
                     PETAPDFLALPNVKVCGGSWLTPDDAVQSGDWARITGLATAANALRG"
     gene            1490..1756
                     /locus_tag="VFV25_03670"
     CDS             1490..1756
                     /locus_tag="VFV25_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEY1392444.1"
                     /translation="MKYVMHYELAPDGLPKAAANFQAHRARLDEFHARGVLLMAGPLG
                     SPPEGAMGVFTTREAAEDFIRGDPFVLNGVVGKWRVIEWNEVLA"
     gene            complement(1763..2146)
                     /gene="gloA"
                     /locus_tag="VFV25_03675"
     CDS             complement(1763..2146)
                     /gene="gloA"
                     /locus_tag="VFV25_03675"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEY1392445.1"
                     /translation="MRLLHTMLRVGDLQRSIAFYTEVMGMKLLRTTDRPEQKYSLAFV
                     GYGHNPEHAEIELTYNYGVDKYELGTAYGHIAIGVPDVYAACEKIRAGGGAVTREPGP
                     VKGGTTVIAFVTDPDGYKIELIERG"
     gene            complement(2159..>2284)
                     /locus_tag="VFV25_03680"
     CDS             complement(2159..>2284)
                     /locus_tag="VFV25_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY1392446.1"
                     /translation="GEPGEIADIVAFLASDAASYMTGQIVYADGGRMSLNYTMPV"
BASE COUNT          340 a          776 c          805 g          363 t
ORIGIN      
        1 accagttcca ggctgcgggc ggccccggct tcgtgctgcg cgagctgctc gacggcggct
       61 ggctgcatga agacgtcgcc acggtgtcca ggcaaggcct gcgtgactac acccgcgtgc
      121 cggcgctgga aggcggcgcg ctgcaatggc gcgagctgcc tgatgcctcg gccgacgaaa
      181 gcatcgtgcg ctgcggagcg gcgcccttca gcgcgacggg cgggctgaag ctgctgaccg
      241 gcaacctggg gcgcagcgtc atcaaggtgt cagccgtgcc cgaggataga cacgtcatcg
      301 aggcgccggc cctggtcttc gacagccagg aggcgctgct cgcagccttc aagggcggca
      361 gcctggagcg cgatttcgtc gccgtggtgc gctaccaggg cccgcaggcc aacggcatgc
      421 cggaactgca caagctcaca ccgccgctgg cggtgctgca gggcaagggc tttcgcgtcg
      481 cgctcgtcac agatggccgc atgagcggcg cctcgggcaa ggtgccggcg gccattcacc
      541 tgagccccga ggcgatggac ggtggcccgc tgggcaaggt gcgcgatggg gatgtgatcc
      601 gtctcgatgc cgtggccggc acgctgcagg ctctggtgcc cgaggccgaa tgggcctcgc
      661 gcgaagccgt ggccccgagt gcggactacc tcggtgagca tgcccatggc ctgggccgcg
      721 aactcttcgc cggcttgcgg cgcaatgtgc gcgccgcgga gcagggcgcc tgcacttggt
      781 tgggctgaat gacgaaagct aagcaatgaa cacactagca ctcgcctccc atggcccggt
      841 gatcccggtc atcgtcatca agcgcctgca ggacgccgtg cccctggcgc gggcgctgct
      901 cgccggcggc gtgcgcgtgc tcgaggtcac gctgcgcacg ccggtggccc tgcaggccat
      961 ggaagcgatc gcgcgcgagc tgcccgaggc catcgttggt gccggcacgg ttcgctcggt
     1021 cgccgatgcc aaggccgcgc tggcagccgg ctgccgcttc gcggtgagcc cgggctacgc
     1081 gagcgacatc ggccgcgcct gccgcgacat cggcctgccc ctgctgcccg gcgtcgccac
     1141 cgccagcgag gtgatgatgg ccaatgccga tggctaccac tttctgaaat tcttcccggc
     1201 cacggcggcc ggcggcatcc ccatgctgaa ggcgctggcc ggtccctttc cggacgtggc
     1261 cttctgcccc accggcggca tcacgcccga gacggcgccg gacttcctcg cgctgcccaa
     1321 tgtcaaggtc tgcggcggct cctggctcac gccggacgac gccgtgcagt ccggcgactg
     1381 ggcgcgcatc acaggcctgg cgacggcggc gaatgcgctg cgtggctgac gggctgggcc
     1441 acgcacctca agcgagcaaa cctgcgagtc acggacaagg gaggccgcga tgaaatacgt
     1501 catgcattac gagctcgcgc ctgacggcct gcccaaggcc gcggcgaact tccaggcgca
     1561 tcgcgcccgg ctcgacgagt tccacgcccg cggcgtgctg ctgatggccg ggccgctggg
     1621 cagcccgccc gaaggcgcga tgggcgtgtt taccacgcgc gaagccgctg aggacttcat
     1681 ccgcggcgac cccttcgtgc tgaacggcgt cgtcggcaaa tggcgcgtga tcgagtggaa
     1741 cgaggtgctg gcttgaagcc ggctacccgc gctcgatcaa ctcgatcttg tagccgtccg
     1801 ggtcggtcac gaaggcgatc acggtcgtgc cgcccttgac cggccccggc tcgcgcgtga
     1861 ccgcgccgcc gccggcgcgg atcttctcgc aagcggcata gacatcgggt acgccgatgg
     1921 cgatgtggcc ataggccgtg ccgagctcgt acttgtcgac gccatagttg taggtcagct
     1981 cgatctcggc atgctcgggg ttgtggccat agccgacgaa ggccagcgaa tacttctgct
     2041 cggggcgatc ggtggtgcgc agcagcttca tgcccatcac ctcggtgtag aaggcgatcg
     2101 agcgctgcag gtcgcctacg cgcagcatgg tgtgcagcag tctcatgacc tgattccctt
     2161 aaaccggcat ggtgtagttg aggctcatgc ggccgccgtc cgcatagacg atctggccgg
     2221 tcatgtagct ggcggcgtcg ctcgccagga aggccacgat gtcggcgatt tcgccgggct
     2281 cgcc
//
LOCUS       DASUCY010000190         2296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_839426, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000190 DASUCY010000000
VERSION     DASUCY010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839426"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="VFV25_03685"
     CDS             <1..567
                     /locus_tag="VFV25_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY1392447.1"
                     /translation="RGLAQLLTDGQIITVYEPASFFWLGGGHLLGLPVSLFVVAVVAG
                     LTALLMRRTALGLFIQAVGINPVAARLAGVHTAAVVFFVYVYCSLAAGLAGLMIASNI
                     KSADANNAGLLLELDAILAVTLGGGQLSGGRFSLAGSLIGALIIQTLTSTIYALGVPP
                     QLSMVVKAVVVFAVCISQSSAWARRRRA"
     gene            680..1330
                     /locus_tag="VFV25_03690"
     CDS             680..1330
                     /locus_tag="VFV25_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017331655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY1392448.1"
                     /translation="MRVSTTRAIALACTSMLIAACSDIERSRNVNNPDVPATTVAQQV
                     CAMCHGVTGVSVSPTFPKLAGQQREYLVAQLSEFKSHSRSDPTGAKFMWGFTHLNEAQ
                     VQGLADYFSSQKPSYGPAGDVKLTEEGRRIFTEGIPDRNVPMCSACHGQQGEGNGQFP
                     RLAGQHADYVVKQLMVFQRTDQRPRGGAMKVVTHDLSEHEMHALATFVQSFQPAAQ"
     gene            complement(1369..1959)
                     /locus_tag="VFV25_03695"
     CDS             complement(1369..1959)
                     /locus_tag="VFV25_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEY1392449.1"
                     /translation="MSSRAALTIIRQEHAALSAVLRSILLLLAQHRRDGSLPDFAALR
                     AMLFYVDEFPEQRHHRKESELLFPKLRARTPLSRELLDRLDQEHARGERSIRDLEHKL
                     LGFEMMGEARRADFEQAAERYVDFYFRHMGLEESEILPLAERVLKPEDWAELDEAFMA
                     NRDPLLGHEPEADYRALFTRIVNTVPAPIGLGPASD"
     gene            complement(2128..>2296)
                     /locus_tag="VFV25_03700"
     CDS             complement(2128..>2296)
                     /locus_tag="VFV25_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY1392450.1"
                     /translation="APQDIGERLLSLACDLGAELLVMGCYSQSRSREFLLGGASRTLL
                     RSMTLPVLMSH"
BASE COUNT          365 a          764 c          788 g          379 t
ORIGIN      
        1 cgcggcctcg cgcagctgct gaccgacggt cagatcatca ccgtctacga gccggcgtcc
       61 ttcttctggc tcggcggtgg gcatctgctc ggcctgcccg tgtcgctctt cgtcgtcgcc
      121 gtcgtcgccg ggttgacggc cctgctgatg cggcgtacgg cgctgggcct cttcatccag
      181 gccgtcggca tcaatccggt cgccgcacgg ctcgcgggcg tgcacacggc ggccgtcgtc
      241 ttcttcgtct acgtctactg cagcctggcc gcgggcctgg ccgggctgat gattgcgtcc
      301 aatatcaaga gcgccgacgc caacaacgcc ggcctgctgc tggagctgga tgccatcctc
      361 gcggtaacgc tgggcggcgg ccagctgagt ggcggcaggt tcagcctggc cggcagcctc
      421 atcggcgcac tgatcatcca gacgctgacg tcgacgatct acgccctggg cgtgccgccg
      481 cagttgagca tggtggtcaa ggccgtcgtc gtcttcgcgg tctgcatctc gcagtcctcg
      541 gcctgggcga ggcgcaggcg cgcatgagaa atccggtgtc aacctgtctt tccgcgggcc
      601 gccgcgccgg gccgctccca agcaaggccc gtcccctgcc ggaggggtgc acggcgtact
      661 cgccgggcga ggtgcagtca tgagagtcag cacgaccagg gccatagcgc tcgcctgcac
      721 gtccatgctc atcgcggcat gttcggacat cgagcggtcg cgcaacgtga acaaccctga
      781 tgtacccgcg acgacggtgg cgcagcaggt ctgcgcgatg tgccacgggg tgacgggggt
      841 gtcggtatcg cccaccttcc cgaagctcgc gggccaacag cgcgagtacc tagtggccca
      901 gctgagcgag ttcaagagtc atagccgctc agatcccacg ggcgccaagt tcatgtgggg
      961 cttcacccat ttgaacgagg cccaggtgca ggggctggcg gactatttct cgtcgcagaa
     1021 gccgagctac gggccggccg gcgatgtcaa gctcaccgaa gaggggcgcc gcatcttcac
     1081 cgagggcatc cccgatcgca acgtgccgat gtgcagcgcc tgccatggcc agcaaggcga
     1141 gggcaacggc cagttcccgc gcctggccgg ccagcatgcg gactatgtgg tcaagcagct
     1201 catggtgttc cagcgcaccg accagcggcc gcgcggcggc gccatgaagg tggtcaccca
     1261 cgacctgtcc gagcatgaga tgcacgcgct cgcgaccttc gtgcagtcct tccagcctgc
     1321 agcgcagtaa aagaagcgag gcttgaggcg ggtgcgtcga ggggagcgtc agtcggatgc
     1381 cggccccaga ccgatcgggg ccggcaccgt gttgacgatg cgggtgaaga gggcgcggta
     1441 gtcggcctcg ggctcatggc ccagcagcgg gtcgcggttg gccatgaagg cctcgtcgag
     1501 ctcggcccag tcctcgggct tcagcactcg ctcggccagc ggcaggatct cgctctcctc
     1561 cagccccatg tgccggaaat agaagtcaac atagcgctcg gctgcctgct cgaagtccgc
     1621 gcgcctggcc tcgcccatca tctcgaagcc caggagcttg tgctccagat cgcggatgct
     1681 gcgctcgccg cgcgcatgct cctggtccag gcggtcgagc agttcgcgcg acaagggcgt
     1741 gcgtgcgcgc agcttgggaa acagcagctc ggattccttg cggtggtggc gctgctccgg
     1801 gaactcgtcg acatagaaca gcatcgcgcg cagcgccgcg aaatccggca ggctaccgtc
     1861 gcgcctgtgc tgcgcaagca gtagcaggat ggagcgcagc accgcgctga gcgcggcatg
     1921 ctcctgcctg atgatggtga gggctgcacg cgagctcatg atttcagtgc tccatggcgc
     1981 tgtgccttcc gcgacgcatg aagcgaggca ccctctgccg ctggcgggag agggttgggg
     2041 tgagggtggg gacgcccaca aaccctcatc ccccgccttc tcccgcgagc gggagaagga
     2101 gctcggcgca gtagcgtcga tgtcatccta atggctcatc aagaccggca gcgtcatcga
     2161 cctgagcagg gtgcgcgagg cgccgccgag caggaattcg cgcgagcggc tctggctgta
     2221 gcagcccatt acgagcagct cggcgccgag atcgcaggcc agggacagca gccgctcgcc
     2281 gatgtcctgc ggggcc
//
LOCUS       DASUCY010000191         3499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_148669, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000191 DASUCY010000000
VERSION     DASUCY010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148669"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1463)
                     /locus_tag="VFV25_03705"
     CDS             complement(<1..1463)
                     /locus_tag="VFV25_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HEY1392451.1"
                     /translation="MATTSLPLSNHPLLRRLQGWLGPNAHLAQGLAIPLLVIMVLAMM
                     VLPLPAFMLDLLFTFNIAMALMVMMVAAYMIKPLDFAAFPSVLLLTTLMRLSLNVASS
                     RVVLLQGHTGTGAAGKVIESFGHFLIGGNFAVGLIVFAILVVINFVVVTKGAERIAEV
                     GARFALDAMPGKQMAIDADLNAGLIDQAEAKRRRAEVGNEAEFFGSMDGASKFVRGDA
                     IAGILILFINMIGGFIIGVAQRDLTMGEAAGSYILLAIGDALVAQIPALLISVAAAMV
                     VSRVGKEHDVGAQILGQMFKTSKSVAVVACVIGLLGVIPGMPHLVFILIGSLLGYLAW
                     WMHKREEEIKNRPPEQAAQPAASDPNAEASWDDLQPVDMLGLEVGYRLIQLVDKERRG
                     DLLARIKGVRRKFAQDVGFLPPAVHIRDNLELKPSSYRITLRGAVVGEGEVFPGMFLA
                     INPGHASTPLIGSSALDPAFGLPATWIEERQRETAQMA"
     gene            complement(1566..2726)
                     /locus_tag="VFV25_03710"
     CDS             complement(1566..2726)
                     /locus_tag="VFV25_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HEY1392452.1"
                     /translation="MADSSSQDKHLPASQRKLQKAREEGQVARSRDLGHFAAVAAGLG
                     LMVAAAPKVIAWLKDGLASALHFDAHTLTSAATMGERLAMSAAQLLWIVLPLGVLVMA
                     VAVASSLLAGGWVWSTQALMPQFSRLNPLAGLPRLLSRQQLGDAGKSCLLAAILFAIG
                     ALYLRSHLDAFGATLGLPLPAAFAAVGDQLLAGLGLVVMALAVFAVIDVPLQRHLHLS
                     RLKMSRQEMKDEFRQLEGNQEIKVKMKARMREMVKRRMLSAVPQASLVVMNPTHYAVA
                     LKYEDGMSAPRVVAKGADLLAMKIRDVAKEAQVPVLQAPVLARALYAHAEIDREIPMA
                     LYSAVAQVLAYVYQLRAALTGRAAMPGELPELQVPAELDPHNDPKRQAGRDE"
     gene            complement(2834..3466)
                     /locus_tag="VFV25_03715"
     CDS             complement(2834..3466)
                     /locus_tag="VFV25_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HEY1392453.1"
                     /translation="MKMDELQGLTPQIAAHASPEIFQQLGAITRQLHDTLQQLGVMPK
                     LQIAADGLPDARSRLNYIASKTAAAANKVLNLVDSAKAEHQQIAEETRRMAAAIVADP
                     VRAVASGSVMNFVGDVEARTKRIDEHLTDIMMAQDFHDLTGQVVAKVVSLATELEDSL
                     VKLLVQAAPPEQAHKVETAYLNGPVVDPEGRTDVVTNQSEVDDLLASLGF"
BASE COUNT          570 a         1184 c         1174 g          571 t
ORIGIN      
        1 gccatttgcg ccgtttcgcg ctggcgttct tcgatccagg tggccggcag gccgaaggcc
       61 ggatcgagcg ccgagctgcc gatcagtggc gtgctcgcat ggcccgggtt gatggcgagg
      121 aacatgcccg ggaagacctc gccttcgccc accacagcgc cgcgcagtgt gatgcgatag
      181 ctgctgggct tgagctcgag gttgtcgcgg atgtgcacgg ccggcggcag gaagccgacg
      241 tcctgcgcga acttgcgccg cacgcccttg atgcgcgcca acaggtcacc gcggcgctcc
      301 ttgtctacca actggatcag gcgatagccc acttccaggc ccagcatgtc gaccggctgc
      361 aggtcgtccc agctggcctc ggcgttgggg tcgctggcgg ccggctgcgc ggcctgctcg
      421 ggcgggcgat tcttgatctc ctcctcgcgc ttgtgcatcc accacgcgag atagcccaac
      481 aggctaccga tgaggatgaa cacgaggtgc ggcatgccag gtatcacgcc tagcaggccg
      541 atcacgcagg ccaccaccgc caccgatttg gaggtcttga acatctgccc caggatctgg
      601 gcgccgacgt cgtgctcctt gcccacgcgc gagaccacca tcgcggccgc caccgagatc
      661 agcagcgccg ggatctgcgc cactaacgcg tcgccgatgg ccagcaggat gtaggagccc
      721 gcggcctccc ccatcgtcag atcacgctgg gccacgccga tgatgaagcc gccgatcatg
      781 ttgatgaata ggatcaggat gccagcgatg gcgtcgccgc gcacgaattt cgaggcaccg
      841 tccatagagc cgaagaactc ggcttcgttg cccacctcgg cgcggcggcg cttggcctcg
      901 gcctggtcga tcaggccggc attgaggtcg gcgtcgatgg ccatctgctt gccgggcatc
      961 gcgtccaggg cgaagcgcgc gccgacttcg gcgatgcgct cggcgccctt tgtgaccacc
     1021 acgaagttga tgaccaccag gatcgcgaac acgatcaggc cgacggcgaa gttgccgccg
     1081 atcaggaaat ggccgaagga ctcgatcacc ttgccggccg cgccggtgcc ggtgtggccc
     1141 tgcagcagca ccacgcgcga ggacgccacg ttgagcgaca gccgcatcag cgtggtcagc
     1201 agcagcaccg acgggaaggc cgcgaagtcc agcggcttga tcatgtaggc cgccaccatc
     1261 atcaccatca gcgccatcgc gatgttgaag gtgaacagca agtccagcat gaaggccggc
     1321 agcggcagca ccatcatcgc cagcaccatg atcaccagca gcggaatggc cagcccctgg
     1381 gccaggtgcg cgttggggcc gagccagccc tgcagccggc gcagcagcgg atggttggac
     1441 agcgggagcg aggtggtggc catgatgggg tcagcgcttg tcaggtgatt cttcccagcg
     1501 ggccgcgagg ctttagcgag ataagcgggg taaggcgggg gcttttcgcg gcgccatgcc
     1561 gcgtcttact cgtctcgccc cgcctggcgc ttcggatcgt tgtgcgggtc cagctccgcg
     1621 ggcacctgta gttccggcag ctcgcccggc atcgccgcgc gccccgtcag tgcagcgcgc
     1681 agctggtaca cataggccag cacctgggcc accgcgctgt acagcgccat cgggatctcg
     1741 cggtcgatct cggcatgcgc gtagagcgcg cgcgccagca ccggcgcctg cagcacgggc
     1801 acctgcgcct ccttcgcgac gtcgcggatc ttcatcgcca ggaggtcggc gcccttggcc
     1861 acgacgcgcg gcgcgctcat gccgtcctcg tacttgagtg ccactgcata gtgggtcggg
     1921 ttcatcacca ccaggctggc ctggggcacg gcgctcagca tgcggcgctt gaccatctcg
     1981 cgcatgcggg ccttcatctt gaccttgatt tcctggttgc cctcgagctg cctgaactcg
     2041 tccttcatct cctgccggct catcttgagg cgcgacaaat gcaggtggcg ctgcagcggc
     2101 acgtcgatca ccgcgaacac ggccagcgcc atcaccacca ggcccagccc ggcgagcagc
     2161 tgatcgccca ccgcggcgaa ggcggcgggc agcggcagcc ccagcgtggc accgaaggca
     2221 tcgaggtggg agcgcagata gagcgcgccg atcgcgaaca ggatggcggc cagcaggcag
     2281 ctcttgcccg cgtcgcccag ctgctggcgc gacaacaggc gcggcaggcc ggccagcgga
     2341 ttgagccgcg agaactgcgg catcagcgcc tgggtcgacc acacccagcc gccggccagc
     2401 aggctgctgg cgacggccac ggccatcacc agaacgccca gcggcagcac gatccacagc
     2461 agctgcgcgg cactcatggc caggcgctcg cccatggtcg cggcggaagt gagcgtgtgc
     2521 gcgtcgaagt gcagcgccga cgcgaggcca tccttgagcc aggctatcac tttgggggcc
     2581 gcggcgacca tcagcccgag gccggccgcc accgccgcga aatgccccag gtcgcgcgag
     2641 cgcgcgacct ggccttcttc acgtgccttc tgcagcttgc gctgcgaggc gggtaaatgc
     2701 ttgtcctggg aagaagagtc ggccatgggc gggaatcacg ctttgtgcgc gattcttccc
     2761 aagggcggcc gaaacctaag ccggataagg gcgcgaagcg agccgctttt cggagcgacc
     2821 cggcttgcga cgcctaaaac ccgaggctgg ccagcagatc gtcgacctcg ctctggttcg
     2881 tcacgacatc ggtgcggcct tcggggtcga ccaccggacc gttgagatag gccgtctcca
     2941 ccttgtgcgc ctgctcgggc ggcgcggcct gcaccagcag cttgaccagg ctgtcctcca
     3001 gctcggtggc cagcgacacg accttggcga ccacctggcc ggtcaggtcg tggaagtcct
     3061 gggccatcat gatgtcggtg aggtgctcgt cgatgcgctt ggtgcgcgct tccacgtcgc
     3121 ccacgaaatt catcaccgag cccgaggcca ctgccctgac cggatcggcc acgatggccg
     3181 cagccatgcg gcgggtttcc tcggcgatct gctggtgctc ggccttggcc gaatcgacca
     3241 ggttgagcac cttgttggcg gcggctgccg tcttgctggc gatgtagttc agcctactgc
     3301 gcgcatcggg caggccgtcg gcggcgatct gcagcttggg catcacgccg agctgctgca
     3361 gcgtgtcgtg cagctggcgc gtgatggcac ctagctgttg aaagatctcg ggcgatgcat
     3421 gcgccgcgat ttgcggcgtg aggccctgga gttcgtccat cttcatgttg cggacctcaa
     3481 gcggcgagct tctgcatga
//
LOCUS       DASUCY010000192         2460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_759193, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000192 DASUCY010000000
VERSION     DASUCY010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759193"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..1056)
                     /locus_tag="VFV25_03720"
     CDS             complement(145..1056)
                     /locus_tag="VFV25_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY1392454.1"
                     /translation="MSKSIVSRFVGCALLASCALLGAVAHAQNLPPTLSRIAADGVIH
                     VSYRESSVPFSYLDNNNKPVGFAMDLCAGIVEAVKARLKKPDLKVDYQLVTSQNRIPL
                     VVNGTVDIECGSTTNNTVRRENVEFAINHFYTGTRLLVKKGSGIKTYADLKGKTVATT
                     TGTTNYQVLRKYDRDHELGLTILPGKDHADSLLLVESDRAVAFAMDDILLYGLAANSK
                     NPAEWEVVGEALQVEPYAIMLRKNDPEFKKLVDDTLLAAIKSGEFERLYRKWLLSPIP
                     PKGINLSVPMSEDLIRHLKAPSDRPAL"
     gene            complement(1140..1778)
                     /locus_tag="VFV25_03725"
     CDS             complement(1140..1778)
                     /locus_tag="VFV25_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY1392455.1"
                     /translation="MLRYLTIPVTAFSQNCSIVWCDETMEAAIIDPGGDLDTLRSEIK
                     RLGVKLKAIWLTHAHIDHAGGTAALAREEQLPIIGPQEADRFWIAGLPQQSAMFGFPP
                     AESFEPTRWLHDGDTVSIGHCTLNVRHCPGHTPGHVVFHSPQIKRAFVGDVLFAGSIG
                     RTDFPGGDFDTLIASITQRLWAMGDDTVFIPGHGPESSFGHERRSNPYVGGT"
     gene            complement(1864..>2460)
                     /locus_tag="VFV25_03730"
     CDS             complement(1864..>2460)
                     /locus_tag="VFV25_03730"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY1392456.1"
                     /translation="AGIGFILANLERTAAKLRKAATHDSLTGCISRGPFEAMLRQARE
                     RARREGTALSLLLMDLDHFKQVNDTHGHEGGDEVLRRFAHAVQSRLRASDILGRMGGE
                     EFAVILPATGAAGAAMIAEELRRVTEALDVPAPQGARIAVTVSVGAAAVEAGAAAQAH
                     DALYGQADSALYASKRAGRNRTTSFSACQCEAKAAAPA"
BASE COUNT          383 a          807 c          829 g          441 t
ORIGIN      
        1 aagggggcgc cgccgcactg gctgtccacc cacccctcgg ggcccacgcg catcaaggac
       61 atggaggaca acctgcccaa ggtgatgccg ctgtacgagc gcgccgagaa gccgccgcgg
      121 cgctacgcgg tcgcgacctg agctctatag cgccggccgg tcgctcggtg ccttcaggtg
      181 cctgatcagg tcttcgctca tcggcacgct caggttgatg cccttgggtg ggatcggcga
      241 cagtaaccat ttacggtaca gccgctcgaa ctcgcccgac ttgatcgccg ccagcagcgt
      301 gtcgtcgacc agcttcttga actccgggtc gttcttgcgc agcatgatcg cgtagggctc
      361 gacctgcagc gcctcgccca ccacttccca ttcggccggg ttcttgctgt tggcggccag
      421 gccatagagc aggatgtcgt ccatcgcgaa ggccaccgcg cggtcggact cgacgagcag
      481 cagcgaatcg gcgtggtcct tgccgggcag gatggtcagg cccagctcgt ggtcgcgatc
      541 gtatttgcgc agcacctggt agttggtggt gccggtcgtc gtcgcgacgg tcttgccctt
      601 gaggtcggca taggtcttga tgcccgagcc cttcttcacc agcagccgcg tgccggtgta
      661 gaaatggttg atcgcgaact cgacgttctc gcgccgcacc gtgttgttgg tggtggagcc
      721 gcattcgatg tcgaccgtgc cgttgaccac cagcgggatg cggttctgcg aagtgacgag
      781 ttggtagtcc accttaaggt cgggcttctt cagcctggcc ttcaccgctt cgacgatgcc
      841 ggcgcacaaa tccatcgcga agcccaccgg cttgttgttg ttgtcgagat agctgaaggg
      901 caccgaggat tcgcgatagc tgacatggat cacgccatcg gcggcgatgc ggctcagcgt
      961 gggcggcagg ttctgcgcat gcgcaacggc tccaagcagc gcgcagctcg cgagcagcgc
     1021 gcagccaacg aaccgggaaa cgatggactt cgacatgacg gcccctgatc ggtttcgaga
     1081 acaccgcagt ctaggcatgc gcgcgcgacc agtggctagg gttagcccaa ggccaggcct
     1141 caagtcccgc cgacgtaggg attgctgcgc cgctcgtggc cgaaactgct ttccggccca
     1201 tgcccgggga tgaagaccgt gtcgtcgccc atggcccaca ggcgctgggt gatgctggcg
     1261 atcagcgtgt cgaagtcgcc gcccgggaaa tcggtgcggc cgatggaccc ggcgaacagc
     1321 acgtcgccga cgaaggcgcg cttgatctgc ggcgagtgaa agaccacatg gcccggcgtg
     1381 tggcctgggc agtggcgcac gttcagcgtg caatggccga tgcttaccgt gtcgccgtcg
     1441 tgcagccagc gcgtcggctc gaagctctcg gcgggcggga agccgaacat cgcgctttgc
     1501 tgcggcagtc cggcgatcca gaagcgatcg gcctcctgcg ggccgatgat gggcagctgc
     1561 tcttcgcgcg ccagcgctgc cgtgccgccg gcatggtcga tgtgggcatg ggtgagccag
     1621 atcgccttga gcttgacgcc cagccgcttg atctcgctgc gcagggtgtc caggtcgccg
     1681 cccgggtcga tgatggccgc ttccatggtc tcgtcgcacc agacgatgga gcagttctgc
     1741 gaaaaggcgg tgacgggaat ggtgaggtag cgcagcatcg ggtgcagctt gttctttccg
     1801 aaccagcttc agttctgaag acctgctttt agcagatgag gctcagggca ggcgccccac
     1861 gagctacgcc ggcgctgccg ccttcgcctc gcactggcag gccgagaagg acgtcgtccg
     1921 gttgcggccc gcgcgcttgg acgcatagag tgcgctgtcg gcttgcccgt acagcgcgtc
     1981 gtgggcctgc gccgcggcac ccgcctccac ggccgccgca cccaccgaca cggtcacggc
     2041 gatcctggcg ccttgcggcg caggcacatc cagcgcttcg gtgacgcggc gcagctcctc
     2101 ggcaatcatc gccgcgcccg ctgcgccggt ggccggcagg atgacggcaa attcctcccc
     2161 gcccatgcgg ccgaggatat cggaggcgcg caggcgggac tgcacggcgt gggcgaatcg
     2221 acgcaacacc tcgtcgccgc cctcgtggcc gtgcgtgtcg ttgacttgct tgaagtggtc
     2281 caggtccatc aacagcagcg acagcgccgt tccttcgcgc cgcgcgcgct cgcgggcttg
     2341 cctgagcata gcctcgaacg gcccccggct gatgcagccc gtaaggctgt cgtgcgttgc
     2401 cgcctttcgc agcttcgcgg ccgtccgctc gagattggcc aggatgaagc ctatgcccgc
//
LOCUS       DASUCY010000193         3253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_36271, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000193 DASUCY010000000
VERSION     DASUCY010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36271"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1846
                     /locus_tag="VFV25_03735"
     CDS             <1..1846
                     /locus_tag="VFV25_03735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY1392457.1"
                     /translation="TEKNDLEDQRMALISDALSLESQISARIEAERQRLKELASKVDA
                     LQTSHDRFAALPEVQQGLHRFWLSITWLDASNRIVVQLPEGIRSSEAQLPKGPPTGLS
                     AHLSVTTTSGATLVARYSPSDMLRQGVPWWLARKYDVRLVDSFGAVIASTAEGKELPH
                     RQSYRVSLSPTLSDSYLELIARDRVKPWYQSLSVLLAAGFVALIAISTWMLRRQMDDV
                     ARAEEAWRTEAAWRGAMEDSLTVGLRARDLEGRLLYVNKTLADMIGYKPEELIGLMPP
                     MPYWPAASHEEIMTRHRRTMAGEAPSEGYEAVWRHRDGRDIQVMIFEARLVDAHGKHI
                     GWMGSTLDITERKRAEERERHQIETMTHQARLTMLGEVASALAHELNQPLTAIASYNA
                     GVINSLRRAGHDDATVLRALQRLGEQARHAGQVVQRIREFLTRRSPQRERCELQNIAR
                     HTLQMLKREVQRHQVAVELSLRPDLPAVWADPVLIEQVVINLVRNAIDELGTVVPPPE
                     GGRQVSLSLTRSGEGYVRLDVCDNGRGLGGLDVDRLCAPFYSTKSEGMGMGLAICRSI
                     LEAHHGVLDAGAAPGGGARFSFALPVYRAPGSGAETTSASPSPEESLQ"
     gene            1843..2463
                     /locus_tag="VFV25_03740"
     CDS             1843..2463
                     /locus_tag="VFV25_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY1392458.1"
                     /translation="MSNELGGVVHLVDDEEPVRDALAFLLGSRGLEVHCYDSGPAFLQ
                     SLQNLKGGQPLHGVIVLDVRMEPMSGLRVHTELVARGVRNPVMFLSGHGDIPMAVEAL
                     KNGAFDFIEKPYSDNALAERVEQALRVESAMRERNAGEAERTARMASLTEREREVMQR
                     VAAGKLNKVIADELGIAVRTVEVHRARVFSKLGVRSAAELATVLLK"
     gene            2587..>3253
                     /locus_tag="VFV25_03745"
     CDS             2587..>3253
                     /locus_tag="VFV25_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY1392459.1"
                     /translation="MQELRQEPRLDHVQCLDTRGLHRMAYWEWGDPGNPQVLVCAHGL
                     TRQGRDFDTLAQALSSHYRVVCPDVVGRGRSDWLADPMGYQLPAYAADMVTLLARLNA
                     SSVDWVGTSMGGLIGMGLASLANTPIRRLVLNDVGPTIEPNSIKRIGDYLGKPLRYAS
                     VDEAADYLWSISKGFGPHTREQWLALTRPQIKPDGDGWVSRYDPRIALAFNGVTPELA
                     AAGE"
BASE COUNT          550 a         1061 c         1127 g          515 t
ORIGIN      
        1 gaccgagaag aacgacctgg aagaccagcg catggcgctg atttccgatg cgctgtcgct
       61 ggagagtcag atcagcgccc gcatcgaggc cgaacggcag cggctaaagg agctggcatc
      121 gaaggtcgat gcactgcaga cctctcacga ccgtttcgca gcgctgcccg aggtgcagca
      181 gggcctgcat cgcttctggc tcagcatcac ctggctcgat gccagcaacc gcatcgttgt
      241 gcaattaccg gaaggcatcc gttccagcga ggcgcagctg cccaaggggc cgcccaccgg
      301 cttgtctgcg cacctttcag tgacgacgac ttcaggggcg acactggtcg cgcgctattc
      361 gcccagcgat atgctgcgcc agggcgtgcc ctggtggctg gcgcgcaagt acgatgtacg
      421 cctggtggac agcttcggcg ccgtgatcgc ctctaccgcg gaaggcaagg aattgccgca
      481 tcgccagagc tatcgcgtca gcctttcgcc gacgctcagc gacagctatc tggagctgat
      541 cgcgcgcgat cgcgtcaagc cctggtacca atcgctgtcg gtgctgctgg cggccggttt
      601 cgtggccctg atcgccatca gcacctggat gctgcgccgc cagatggatg acgtggcgcg
      661 cgccgaggaa gcctggcgca ccgaggccgc atggcgcggc gcgatggagg actccctgac
      721 ggtgggcctg cgcgcacgcg acctcgaagg ccggctgctg tacgtcaaca agacgctagc
      781 cgacatgatc ggctacaagc ccgaggagct gatcggcctg atgccgccga tgccttattg
      841 gcccgcggcc tcgcacgagg agatcatgac ccggcatcgc cgcaccatgg ccggcgaggc
      901 gcccagcgaa ggctacgagg cggtctggcg ccaccgcgac gggcgcgaca tccaggtgat
      961 gatcttcgag gcgcgcctgg tcgatgcgca cggcaagcac atcggctgga tgggctccac
     1021 cctcgacatc accgagcgca agcgcgccga ggagagggag cggcaccaga tcgaaaccat
     1081 gacgcatcag gcgcgcctga ccatgctggg cgaggtggcc tcggcactcg cgcacgagct
     1141 gaaccaaccg ctgacggcca tcgccagcta caacgccggt gtgatcaatt cgctgcgccg
     1201 ggccgggcac gacgacgcca cggtgctgag ggctctgcag cgcctgggcg agcaggcccg
     1261 gcacgccggc caggtggtgc agcgcatccg cgaattcctg acgcggcgca gcccgcaacg
     1321 cgaacgctgc gagctgcaga acatcgcgcg ccacaccttg cagatgctca agcgcgaagt
     1381 gcagcgccac caggtggcag tggagctgtc cttacgcccg gacctgccgg cggtctgggc
     1441 cgaccccgtg ctgatcgagc aggtggtgat caacctggtg cgcaacgcga tcgacgaact
     1501 gggcactgtg gtgccgccgc ccgagggagg gcgtcaggtc agcctgagcc tgacccgcag
     1561 cggcgaaggc tatgtgcggc tggatgtctg cgacaacggc cgcggcctcg gcgggctcga
     1621 cgtcgatcgc ctctgtgcgc cgttttattc gaccaagagc gagggcatgg gcatgggcct
     1681 ggcgatttgc cgctccatcc tggaggccca tcacggcgtg ctggacgccg gcgcggcacc
     1741 cggtggcggg gcacggtttt ccttcgcctt gccggtatat cgggcgcctg gctccggcgc
     1801 cgagacgacc agcgccagcc cgtcgccgga ggagagcctg caatgagcaa tgagcttggt
     1861 ggtgtcgtgc acttggtgga cgacgaggag ccggtgcgcg atgcgctggc cttcctgctg
     1921 ggctcgcgcg gcctggaggt gcattgctat gacagcgggc cggctttttt gcagagcctg
     1981 caaaacctga agggggggca gcctctgcac ggtgtgatcg tgctcgatgt gcgcatggag
     2041 ccgatgtccg gcctgcgcgt gcacaccgag ctggtggcgc gcggcgtgcg caacccggtg
     2101 atgttcctgt cgggccacgg cgacatcccg atggcggtcg aagcgctgaa gaacggcgcc
     2161 ttcgacttca tcgagaagcc ctacagcgac aacgccttgg ccgagcgcgt cgaacaggcc
     2221 ctgcgcgtcg aatcggcgat gcgcgagcgc aatgccggcg aggccgagcg caccgcccgc
     2281 atggccagcc tcacggagcg cgagcgcgaa gtcatgcagc gcgtggccgc cggcaagctg
     2341 aacaaggtga tcgccgacga gctcggcatc gcggtgcgca ccgtcgaggt ccatcgcgcc
     2401 cgggtcttca gcaagctcgg cgtgcgctcg gcggccgagc ttgcgacggt tcttttgaaa
     2461 taggccgcgt ggagcgcccg cttcgctgcg tgcggtgctg gtccgccgca tgaggatatt
     2521 tgccgacttg agccgggcct tggataggat gcgtcccaga agctatcgaa aaagaggacg
     2581 ccgcaaatgc aagaactccg gcaagaaccc cgtctcgacc atgtgcagtg cctggatacc
     2641 cggggcctgc atcgcatggc ttattgggaa tggggcgatc ctggcaatcc gcaagtgctg
     2701 gtctgcgcac acggcctgac gcgtcagggg cgcgacttcg acaccctggc ccaggccctc
     2761 agctcgcatt accgcgtggt ctgtcctgac gtagtcgggc gcggtcggtc ggactggctg
     2821 gccgacccga tgggctacca attgccggcc tatgcggcgg acatggtgac gctgctggcg
     2881 cggctgaacg caagctcggt cgattgggtc ggcacctcga tgggtggcct gatcggcatg
     2941 ggcctggcct cgcttgcgaa cacgccgatc cgccgcctgg tgctcaacga cgtcggcccc
     3001 acgatcgagc ccaattcgat caagcgcatc ggcgactacc tcggcaagcc tttgcgctat
     3061 gccagcgtcg acgaggcggc ggactatcta tggagcatct ccaagggctt cggcccgcac
     3121 acccgggagc aatggctggc gctgacgcgc ccgcagatca agcccgacgg ggacggctgg
     3181 gtcagtcgct acgacccgcg cattgcattg gccttcaacg gtgtgacgcc ggagctggcg
     3241 gccgccggcg agg
//
LOCUS       DASUCY010000194         5051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_919769, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000194 DASUCY010000000
VERSION     DASUCY010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 5051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5051
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919769"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(208..1002)
                     /locus_tag="VFV25_03750"
     CDS             complement(208..1002)
                     /locus_tag="VFV25_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEY1392460.1"
                     /translation="MNFGLIIIGDEILSGKRQDKHLAKCIELLAERGLALSWAHYVGD
                     EPARITAELKAAFASGDAVFSCGGIGATPDDHTRQCAAAALGRPLSLHPQARELILER
                     MRDVAAEQGLPYEPERPDNVHRLNMGVFPQGASLIPNPYNKIPGFSVGELHFLPGFPV
                     MAWPMMAWVLDQRYAHLHRRDAKSERSVIVYGSMEATLTPLMEAVEAAHAGVKVFSLP
                     SVDDPKYGRHIELGVKGDHAAVATAYLMLLDGLRQHGAKLGPELVR"
     gene            complement(999..1889)
                     /locus_tag="VFV25_03755"
     CDS             complement(999..1889)
                     /locus_tag="VFV25_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HEY1392461.1"
                     /translation="MKLLLDSFWRAAAYCLHPRVILLSLLPLLVAGALTGLLGYFYWE
                     GALQAVRSTLESWSLVEAVMGWLDAVGATGFRAVIAPLILVALTVPLIVVVSLLLVAV
                     MMTPAIVKLVADRRFAQLERRHGEPLWRSLLWSTFATLTALLALVISIPLWLVPPLVL
                     ILPPLIWGWLSYRVMSFDALADHASREERRGVMHEHRWPLLAIGVITGYLGAAPSLVW
                     AVSAAALIFAPLLIVASVWLYTLVFAFASLWFTHYCLAALQTWRATHEPEPAPVPAAP
                     AADPAALEMQAAMPPLPPAL"
     gene            2018..2506
                     /locus_tag="VFV25_03760"
     CDS             2018..2506
                     /locus_tag="VFV25_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY1392462.1"
                     /translation="MSKIEARLQAMGLTLPPPLRPPAGIVLPFRFVRIVGQRALISGH
                     GPQDPDGSFAQPLGKLGREVSLEQGYRAARLTALSILGSLQRTLGDLDRISAWSRVFG
                     MVNSAPGFNRQPSVINGFSDLILEVFGPEIGAHSRSAVGMAELPFDLPVEIEGEVEID
                     QG"
     gene            complement(2519..>5051)
                     /locus_tag="VFV25_03765"
     CDS             complement(2519..>5051)
                     /locus_tag="VFV25_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007839234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY1392463.1"
                     /translation="SLQALAWRQLWRDFRAGELRLLLVAVMLAVAALTAVGFFADRLN
                     TGLARDARQLLGGDAIVASDLPTPQPLLQLARRLGLATATTASFPSMARAGDAQGGAS
                     KLVAVKAVSDNYPLRGRLRLSQGPGGTEQDVAAAPPRGSVWVDAGLLDALKLKPGDEL
                     LLGDARLRIARLIVIEPDRGAGFMSFAPRVMLNEADLPATGLIQPASRISYRLAVAAN
                     GSAGTGDEAAVSQFVAKAEAMIQQQSLRGVRVESLQSGRPEMRQTLDRAAKFLNLVAL
                     LAALLAAVAVGIAARDFAAAHLDDCAMLRVLGQSQRRIAGAYALEFALVGCIASLLGV
                     LLGLAVHFVFVWLLAGLVDAALPPPGIWPALFGLGVGLTLLLGFGLPPVLQLARVPAL
                     RVIRRELGELKASSAGALLAGFMGFAALLLAVASDLKLGLIAVGGFVAAAAVFAALSY
                     LALLALKRAVPESRAPRWLVLATRQLAARPGFAVLQTSALSVGLLALVLLVLLRTDLI
                     ASWRQATPPDAPNRFVINVLPEQGEAFQRQLRAAGVSRYDWYPMIRGRLVAVNGKSVG
                     PESYAEERAQRLVDREFNLSYAAQAPAHNQIAAGAWSPGAPDAISVEEGIAGTLKLKL
                     GDTLSFDIAGITQQGRITSLRKLDWSSMRVNFFVMFAREQMPDLPLTYISAFRAPADP
                     GFDNALARSFPNITDIDVSAQLAQVQRVLDQVIRAVEFLFGFTLATGLVVLFAAVSAT
                     REERAREFAVMRALGASAGLLERVQRAELLGVGALAGALASLAAVAVGWALARYVFEF
                     SWNASPWVVPAGAMAGAMLALAAGWWGLREVLRRPVVETLRRAAE"
BASE COUNT          872 a         1784 c         1728 g          667 t
ORIGIN      
        1 agatgcgcgg gtggatgggg cccggaagcc atggggcgat agccccgccg ggcgcacggc
       61 aatgtagcag aaaccatgcc cgcgacagca tgatgacggc ctggcgcact gatgcgcagc
      121 cccatgcggt gggcattggt gccgagaggc ggtcgcgcac cgatctggcg cagattcgcg
      181 caccagtttg gcgcctcaat tcggacatca gcgcaccagc tcggggccca gcttggcgcc
      241 atgctgacgc agcccatcca gcagcatcag ataggcggtc gcgacagccg cgtgatcgcc
      301 cttcacgccc agttcgatgt gccggccgta tttcggatcg tcgacgctgg gcaggctgaa
      361 gaccttgacg ccggcgtgcg cggcctcgac cgcctccatc agcggcgtca gcgtggcctc
      421 catggagccg tagacgatga ccgaacgctc gctcttcgca tcccgcctgt gcagatgcgc
      481 atatcgctgg tccagcaccc aggccatcat cggccaggcc atgaccggaa agccgggcag
      541 gaagtgcagc tcgccgacgc tgaacccggg aatcttgttg tagggattgg ggatgaggct
      601 cgcgccctgc gggaacacgc ccatgttcag ccggtgcacg ttgtcgggcc gctcgggctc
      661 atagggcagg ccctgctcgg cggcgacgtc gcgcatgcgc tccaggatga gctcgcgcgc
      721 ctgcgggtgc agcgagagcg gacggcccag ggcggcagcg gcgcattggc gcgtgtgatc
      781 gtccggcgtg gcaccgatgc cgccgcagga gaagaccgca tcgccgctgg cgaacgccgc
      841 cttgagctcg gccgtgatgc gcgccggctc gtcgcccacg tagtgcgccc agctcaaggc
      901 caggccgcgc tcggcgagca gctcgatgca cttggcaagg tgcttgtcct ggcgtttgcc
      961 ggacaggatc tcgtcgccga tgatgatgag gccgaagttc acagcgcagg cggcagcggt
     1021 ggcatggcgg cctgcatctc cagcgcggcg gggtccgccg ccggtgccgc cggtactggc
     1081 gccggctcgg gctcatgcgt agcacgccag gtctgcagcg cggccaggca gtaatgcgtg
     1141 aaccacagcg aagcgaaggc gaataccagc gtgtagagcc agaccgaggc gacgatgagc
     1201 agcggcgcga agatcagcgc cgctgcgctg accgcccaga ccagcgaggg cgcagcgccc
     1261 aggtagccgg tgatcacgcc tatggccagc aacggccagc gatgctcgtg catcacgccg
     1321 cggcgctcct cgcgcgaggc atggtcggcc aaggcgtcga agctcatcac gcgatagctc
     1381 agccaacccc agatcagcgg cggcaggatc agcaccagcg ggggcaccag ccacagcggg
     1441 atgctgatga cgagcgccag cagggccgtc agagtggcaa acgtggacca cagcaggctg
     1501 cgccacagcg gttcgccatg gcggcgctcc agctgcgcga agcgccggtc ggccaccagc
     1561 ttcacgatcg ccggcgtcat cattaccgcc accagcagca gcgagacgac cacgatcagc
     1621 ggcacggtca gtgcgactag gatcagcggc gcgatcacgg cgcgaaagcc ggtggccccc
     1681 acggcatcga gccagcccat cacggcttcc acgagcgacc agctctcaag cgtgctgcgc
     1741 accgcctgca gggcgccttc ccagtagaaa tagcccagca ggccggtcag cgcacccgcg
     1801 acaagcagcg gcagtagcga caacaggatc acgcgcgggt gcaggcaata ggccgcagcg
     1861 cgccagaagg aatcgagcag aagcttcatg ccggggagca taaacgctgc cggcgcggcg
     1921 cgccgaatac ccgtctccgt ggacgagttc acgacctaca ctgggaatca tgccgtgttc
     1981 aggcggcgcg gttcagcatc gaaggaggag gcccagcatg tccaagatcg aagccaggct
     2041 gcaagccatg gggctcacgc tcccaccacc ccttcggccg cctgccggga tcgtgctgcc
     2101 cttccggttc gtcaggatcg tcggccagcg ggccttgatt tccggccacg ggccgcagga
     2161 cccggacggg tcattcgcgc agcccctggg caagctaggc cgtgaggttt cgctggagca
     2221 gggctatcgg gccgcgcggc tcacggcgct gtccattctc ggcagcctgc agcgcacgct
     2281 cggtgatttg gatcgcatct ccgcctggtc tcgggtgttc ggcatggtga actctgcccc
     2341 cggcttcaac cggcagccca gtgtcatcaa tggcttctcc gatctgatcc tggaggtctt
     2401 tggccccgag atcggcgccc acagccgcag cgctgtcggc atggccgagc tgccgttcga
     2461 cttaccggtg gaaatcgaag gcgaggtcga aatcgaccag ggctgaacca aagcctgctc
     2521 attccgccgc gcggcgcagc gtctccacca cgggccggcg cagcacttcg cgcaagcccc
     2581 accagccggc cgccagcgcg agcatggcgc ccgccatcgc gccggccggc accacccagg
     2641 gcgaggcatt ccagctgaac tcgaacacat agcgagccag cgcccagccc acggccacgg
     2701 cggcaagcga ggccagcgcg cccgccagcg cgcctacgcc cagcaactcg gcgcgctgca
     2761 cgcgctccag caagccggcg ctggcaccga gggcgcgcat gacggcgaac tcccttgcac
     2821 gctcttcgcg cgtcgcactg acggccgcga acagcacgac caaaccggtg gcgagcgtga
     2881 agccgaacag gaactccacc gcgcgtatca cctggtccag cacgcgctgc acctgcgcca
     2941 gttgcgccga gacgtcgata tcggtgatgt tggggaagct gcgggccagg gcgttgtcga
     3001 agcctgggtc ggccggcgcg cggaatgcag agatataggt cagcggcagg tcgggcatct
     3061 gttcgcgcgc gaacatcacg aagaagttga cccgcatcga gctccaatcc agcttgcgca
     3121 ggctggtgat gcggccctgc tgcgtgatgc cggcgatgtc gaagctcagt gtgtcgccga
     3181 gcttgagctt gagggtgccg gcgatgccct cttcgacgct gatcgcatcg ggcgcacccg
     3241 ggctccatgc tccagccgcg atctggttgt gggccggcgc ctgtgcggca tagctcaggt
     3301 tgaactcgcg atccaccagg cgctgggcgc gctcctcggc ataactctcg ggccccacgc
     3361 tcttgccgtt gacggccacc agccggccgc ggatcatcgg ataccagtcg tagcggctca
     3421 cgccggcggc acgcagctgg cgctggaagg cctcgccctg ttcgggcagc acattgatga
     3481 cgaagcggtt gggcgcgtcc ggcggcgtgg cctggcgcca gctcgcgatg aggtcggtgc
     3541 gcagcagcac cagcagcacc agggccagca gccccacgct gagcgctgac gtctgcagca
     3601 ccgcgaagcc ggggcgcgcg gcgagctggc gcgtggccag caccagccag cgcggcgcgc
     3661 gcgactcggg cacggcccgc ttcagcgcca gcaaggccag ataggacagg gccgcgaaga
     3721 ccgccgccgc cgcgacgaag ccgcccaccg cgatcaatcc cagcttgaga tccgaagcca
     3781 cggccagcag cagggcggca aaacccatga agcccgcgag cagcgcgcct gccgagcttg
     3841 ccttgagctc gcccagctcg cgcctgatca cccgcaacgc ggggacgcgc gccagctgca
     3901 gcaccggcgg caggccgaaa cccagcagca gcgtcaggcc cacgcccagg ccgaacagcg
     3961 cgggccagat gccgggcggc ggcagcgccg catcgaccaa accggccagc agccagacga
     4021 agacgaagtg cacggccagc ccgagcagca cgccgagcag gctggcgatg cagcccacca
     4081 gcgcaaactc cagcgcatag gcacccgcga tgcgccgctg cgactggccc agcacgcgca
     4141 gcatcgcgca atcgtccagg tgcgcggccg cgaaatcgcg tgcggcgatg cccacggcga
     4201 cggccgcgag cagcgccgcc agcagcgcga cgaggttgag aaatttcgcc gcgcgatcca
     4261 gggtctggcg catctcgggt cggccgctct gcagcgattc gacgcgcacg ccgcgcagcg
     4321 attgctgctg gatcatggcc tcggccttgg ccacgaactg gctcaccgct gcctcatcgc
     4381 cagtgccagc gctgccattg gccgccacgg ccaaccggta gctgatacgg ctggccggct
     4441 ggatcagccc cgtggccggc agatcggcct cgttgagcat cacgcgcggc gcgaagctca
     4501 tgaagccggc gccgcgatcg ggttcgatga cgatgaggcg cgcgatgcgc agcctggcat
     4561 cgcccagcag cagctcatcg cccggcttga gcttgagggc gtcgagcagg ccggcatcca
     4621 cccacacgct gccacgcggc ggcgctgcag ccacgtcctg ctcggtgccg cccggccctt
     4681 gcgagagccg cagcctcccg cgcagcgggt agttgtcgct cactgccttg acggccacca
     4741 gcttgctggc cccgccctgc gcgtcgccgg ccctggccat gctcgggaaa ctggctgtgg
     4801 tcgcggtggc gagccccagg cgccgcgcca gctgcagcag cggctgcggg gtcggcagat
     4861 cgctggcgac gatggcatcg ccacccagca gctggcgcgc gtctcgcgcc aggccggtgt
     4921 tgagccggtc ggcgaagaag cccaccgccg tcagcgctgc tacggcgagc atcacggcca
     4981 ccagcagcag acgcagctcg ccggcgcgaa agtcgcgcca cagctggcgc caggccaggg
     5041 cttgcagcga g
//
LOCUS       DASUCY010000195         3167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1160857, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000195 DASUCY010000000
VERSION     DASUCY010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160857"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VFV25_03770"
     CDS             <1..577
                     /locus_tag="VFV25_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY1392464.1"
                     /translation="SLEGQFQAQEQAARLIAGLSLVSLALMFVVLYSRYRSAVLAGLI
                     MANIPLALIGSVVAMWIAGVSLSVASMVGFITLAGIATRNGILKISHYINLCRFEGEV
                     FGQQMIVRGSLERLTPVLMTALVAAFALTPLLLSPQMPGKEILHPVAVVIFGGLISST
                     LLDTLLTPLAYAAVGRRATERLLQGGADDAI"
     gene            669..1064
                     /locus_tag="VFV25_03775"
     CDS             669..1064
                     /locus_tag="VFV25_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392465.1"
                     /translation="MRAIKFKHFIRCALMGAALVLGIVPAAQAHGDGTPKHGGVVRSA
                     NDISFELASAATGATVYLEDHGEPLAAAGFSGKLTVLRNSQKMEKELQPGKANELVAT
                     GMQLASGDRAVVVLTTPQKQTVSVRFALP"
     gene            1322..>3167
                     /locus_tag="VFV25_03780"
     CDS             1322..>3167
                     /locus_tag="VFV25_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEY1392466.1"
                     /translation="MTKTKAFRRALIPAAASLICSSLAWAQASPPAPEGAASTASTGT
                     PASGASQSSAATPSSGTATLDTVSVTGKKQPYRNLSATGATKNDALIKDLPMSVRVVN
                     AEVLKDAGVNDLAGALDFGSSISKANNFGGMWDSYAMRGFTGSPDFGSDYMVNGFNSS
                     RGYNGLRDGANTASVEILKGPSSALYGRGEPGGTVNITTKKPLFQPAATLEGSIGSFN
                     TWRTAADLNTPLGETVAFRFNMAHEEGKSFRDTIHKQHTLVAPSLLWMISPDTTLSYE
                     AELLEQKTPLDRGVVAVKGQLGLIPVSRFLGEPRDGDITIRSGGHQVFLQHYFNDDWS
                     LQTGLSYRESQLKGLATEAWRWDPINDGRTLDRQLRKRDYGAIDASGRFEVLGKASTL
                     GVKHNMLFGVDSYRFVDNRVQSRGRSAAFPYSIDIYDPVYGVSQPAPLKTIQNTVEQQ
                     YSTGYYAQDQLEFTPQWKALAGVRYDSYRQGVLNRNITPNAMTSQNLSATSPRVGVVY
                     QPTTTVSLYATAARGFRPNSGISRQGDAFPPERSRSYEVGAKYDSADSKYSGTVALYQ
                     ITKQNVLTFNPANTVFSLLVGEVQSKGVELDFSGEIARNLRLTAAYAFT"
BASE COUNT          574 a         1098 c         1001 g          494 t
ORIGIN      
        1 cagcctcgaa gggcagttcc aggcgcagga gcaggccgcg cggctgatcg ccggcctgtc
       61 gctggtgtcg ctcgcgctga tgttcgtggt gctctattcg cgctaccgct cggcggtgct
      121 ggccgggctc atcatggcca acatcccgct cgcgctgatc ggctcggtgg tcgcgatgtg
      181 gatcgccggc gtgagcctgt cggtggcctc gatggtgggc ttcatcaccc tggccggcat
      241 cgcgacgcgc aacggcatcc tgaagatcag ccactacatc aacctgtgcc gcttcgaggg
      301 cgaggtcttc ggccagcaga tgatcgtgcg cggctcgctg gagcggctca cgccggtgct
      361 gatgacggcg ctggtggccg ccttcgcatt gacgccgctg ctgctgtccc cgcagatgcc
      421 gggcaaggag atcctgcatc cggtcgcggt ggtcatcttc ggcgggctga tcagctcgac
      481 cctgctcgac acgctgctca cgccgctcgc ctatgcggcg gtggggcgcc gcgctacgga
      541 gcgccttttg caaggaggag ccgacgacgc catctgaccc gtcagcagcg acttcaagca
      601 ttgactcaat cacagggaga ggtctcgcgg cgcaaatttt tcgccgcgtt ccatcggagt
      661 acatcgccat gagagcaatc aaattcaagc acttcatccg ttgcgccctg atgggcgcgg
      721 cgctggttct cggcatcgtg ccggccgcgc aggcgcacgg tgacggcacg cccaagcacg
      781 gcggcgtcgt gagatcggcc aacgacatca gcttcgagct ggcctcggcg gccacgggag
      841 ccaccgtcta tctggaagac cacggagagc cgctggcggc agcaggcttc agcggcaagc
      901 tgacggtgct gcgcaatagc cagaagatgg aaaaggagct gcagccgggc aaggccaacg
      961 agctggtggc cacgggcatg cagcttgcat cgggcgaccg tgccgtcgtg gtgttgacca
     1021 cgcctcagaa gcagaccgtg tcggtgcgct tcgcgctgcc atgaacccat gaactgatgg
     1081 gccgcgggtg caacgggcgc ccggaccaag cctcgcctga aaccgcgctc ctcgcgcggc
     1141 gttttgccaa gccttgcggc ggggcgggga tggttcgcgc ctatcgaacg ccggaaggcc
     1201 ctctgcgtga gttccccgat ctcgcaatcc acgactcgat tcgtacaaga cggcgagcgc
     1261 tgcgtgcatg aatcttcgtc ccgaacgctt cctgctcaac ccaatgaaag aacggccaat
     1321 catgaccaag acaaaagctt tccgccgggc actgatcccc gcagctgcca gcctgatttg
     1381 cagctccctc gcatgggcac aggcgagccc gcccgcgccc gaaggcgccg catcgacggc
     1441 ctccaccggc acgccggctt ccggcgcatc gcagtcctcc gccgcaacgc cctcctccgg
     1501 gaccgccacg ctggacaccg tttccgtcac gggcaagaag caaccctatc gcaacctgtc
     1561 ggccaccggc gccaccaaga acgacgcgct catcaaggac ctgccgatga gcgtgcgcgt
     1621 ggtcaacgcc gaggtgctga aggacgccgg cgtcaacgac ctcgccggcg cgctcgactt
     1681 cggcagcagc atctccaagg ccaacaactt cggcggcatg tgggacagct acgcgatgcg
     1741 cggcttcacc ggcagcccgg acttcggctc ggattacatg gtcaacggct tcaactcgag
     1801 ccgcggctac aacggcctgc gcgacggcgc caacaccgct tcggtcgaaa tcctcaaggg
     1861 cccgtcgtcg gcgctgtacg gccgcggcga accgggcggc acggtcaaca tcaccaccaa
     1921 gaagccgctg ttccagccgg ccgccacgct ggaaggcagc atcggcagct tcaacacctg
     1981 gcgcaccgcg gccgacctga acaccccgct cggcgagacg gtcgcctttc gcttcaacat
     2041 ggcgcatgaa gaaggcaaaa gcttccgcga cacgatccac aagcagcaca cgctagtcgc
     2101 gccttcgctg ctgtggatga tctcgccgga caccacgctg tcgtacgagg ccgagctgct
     2161 ggagcagaag acgccgctgg atcgcggcgt ggtcgcggtc aagggccagc tgggcctgat
     2221 tcccgtgtca cgcttcctgg gcgagccgcg cgacggcgac atcaccatcc gctcgggtgg
     2281 tcaccaggtg ttcctgcagc attacttcaa cgacgactgg tcgctgcaga ccggactcag
     2341 ctaccgcgag agccagctca agggcctggc taccgaagcc tggcgctggg acccgatcaa
     2401 cgacggccgc acgctggacc ggcaactgcg caagcgcgac tacggcgcga tcgacgcctc
     2461 gggccgtttc gaggtgctgg gcaaggccag caccttgggc gtcaagcaca acatgctgtt
     2521 cggcgtggat agctaccgct tcgtggacaa ccgcgtgcag tcgcgcggcc gctcggccgc
     2581 cttcccgtac agcatcgaca tctacgaccc ggtgtacggc gtgtcgcagc cggcgccgct
     2641 gaagacgatc cagaacaccg tcgagcagca gtactcgacg ggctactacg cgcaggacca
     2701 gctcgagttc acgccgcaat ggaaggcgct ggccggcgtg cgctacgaca gctaccgcca
     2761 gggcgtgctc aaccgcaaca tcacgcccaa cgcgatgacc tcgcagaact tgagcgcgac
     2821 cagcccgcgc gtgggcgtgg tctaccagcc aaccaccacc gtgtcgctgt atgccacggc
     2881 cgccaggggc ttccgcccta acagcggcat cagccgccag ggcgatgcct tcccgccgga
     2941 gcggagccgc tcctacgagg tgggcgccaa gtacgacagc gccgacagca agtacagcgg
     3001 caccgtggcg ctctaccaga tcaccaagca gaacgtgctc accttcaatc cggccaatac
     3061 cgtcttctcg ctccttgtgg gcgaggtgca gagcaagggt gtcgagctgg acttctccgg
     3121 cgagatagcc aggaacctgc ggctgaccgc cgcctacgca ttcaccg
//
LOCUS       DASUCY010000196         2663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_132697, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000196 DASUCY010000000
VERSION     DASUCY010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132697"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1089
                     /locus_tag="VFV25_03785"
     CDS             <1..1089
                     /locus_tag="VFV25_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEY1392467.1"
                     /translation="TPWFTTALELFLGPQAAQPLSSLLSFLGVASLFILFADLFPKRL
                     SMIEPERLALRVVGTMQGLMLVLKPVVWVFNGLTNLLFKLLGLPVQRDERITPDDILA
                     MADAGAQAGVLQRREHQVISNVFELDTRTVQSVMTQRERIVYLLQDDPEDLIRSRVAA
                     EPHSTYLVCDQHIDQIAGYVDATGLFQRVLNEQPIRLRGEASQGLLHKVLVVPDRLTL
                     SEMLTQFRQAHEDFAVIVNEYSLVVGVITLNDMMSTVMGSLVAPHAEEQIVRRDDGSW
                     LMDGLTPIFDVLRAISADAGELPQRGQYDTLAGFLMVMLRRVPRRTDCVIWSNHRFEV
                     MDVDSYRIDQVMVTRLADAGGVPLEAAA"
     gene            1092..1343
                     /locus_tag="VFV25_03790"
     CDS             1092..1343
                     /locus_tag="VFV25_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HEY1392468.1"
                     /translation="MGDALPPLPAIQLGRYRHYKGGLYAVIGVARHSETHEPQVVYRP
                     LYGEGALWVRPYAMFIESVLIDGVAQPRFAYIEASSISR"
     gene            complement(1313..2461)
                     /locus_tag="VFV25_03795"
     CDS             complement(1313..2461)
                     /locus_tag="VFV25_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013802706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEY1392469.1"
                     /translation="MSTIFYITQVQFDFGAVRQLAAECERVGIQRPLIVTDAGVRAAG
                     VLQPALDALKDLPLAVFDATPSNPTEAAVRSAHEVWKANRCDGLVAVGGGSSIDLAKG
                     LAIMATHPGPLKTYATIEGGSPKITAAVAPLIAVPTTAGTGSEVARGAIVIVDDGRKL
                     GFHSWHLVPRSAICDPELTLGLPPLLTAATGMDAIAHCMETFMAPPFNPPADGIALDG
                     LERGWAHIEAATRDGQDRDARRQMMSASMQGAMAFQKGLGCVHSLSHSLGGINPRLHH
                     GTLNAVFLPAVVRFNAQAESVKAERRLERMAHAMSLADAGDIPEATSKAIRAMNARLG
                     LPTGLAAMGVERSWFDKIIEHALLDHCHKTNPRLASAEDYREMLEASM"
BASE COUNT          407 a          884 c          909 g          463 t
ORIGIN      
        1 acgccctggt tcaccacggc gctggagctc ttcttggggc cgcaggcggc gcagccgctg
       61 agctctttac tgtccttcct gggcgtggct tcgctcttca tcctgtttgc tgatctcttt
      121 cccaagcggc tgagcatgat cgagcccgag cgcttggcgt tgcgcgtggt cggcactatg
      181 caggggctga tgctggtgct caaacccgtg gtctgggtct tcaacggcct gaccaacctg
      241 ctcttcaagc tgctgggcct gccggtgcag cgcgacgagc gcatcacgcc cgacgacatc
      301 ctcgcgatgg ccgatgccgg cgcccaggcc ggcgtgctac agcggcggga gcaccaggtg
      361 atctcgaatg tcttcgaact cgacactcgc accgtgcaga gcgtgatgac ccagcgcgag
      421 cgcatcgtct acctgctgca ggacgacccg gaagacctga tacgcagccg cgtcgccgcc
      481 gagccgcatt cgacttatct ggtctgcgac cagcacatcg accagatcgc aggctatgtc
      541 gatgcgaccg gcctgttcca gcgcgtgctg aacgagcagc ccatacgcct gcgcggcgag
      601 gcgtcccagg gcctgctgca caaggtgctg gtcgtgccgg accggctcac gctgtcagag
      661 atgctgacgc agttccgcca ggcccacgag gacttcgccg tcatcgtcaa cgaatacagc
      721 ctcgtggtcg gcgtgatcac gctcaacgac atgatgagca ctgtgatggg cagcctggtc
      781 gcgccgcacg ccgaggagca gatcgtgcgc cgcgatgatg gctcctggct catggacggc
      841 ttgaccccca tcttcgacgt gctgcgtgcc atcagtgccg acgcaggcga gttgccgcag
      901 cgcggccagt acgacacgct ggcgggcttc ctgatggtga tgctcaggcg cgtgccacgg
      961 cgcaccgatt gcgtgatctg gagcaatcac cgcttcgagg tgatggacgt ggacagctac
     1021 cgcatcgacc aggtgatggt gacgcgtctg gccgatgccg gcggcgtgcc gctggaggcg
     1081 gcggcatagc tatgggtgac gcactcccgc cgctcccggc gatccaactc ggccgctacc
     1141 ggcactacaa gggcgggctg tacgcggtga tcggcgtggc ccggcacagc gagacgcacg
     1201 agccgcaggt cgtatatcgg ccgttgtacg gcgagggagc gctgtgggtg cggccctatg
     1261 cgatgtttat cgaaagcgtg ttgatcgacg gtgtcgcgca gccgcgcttt gcctacatcg
     1321 aggcttcgag catctcgcgg tagtcctcgg ccgaggccag ccgtggattg gtcttgtggc
     1381 aatggtcgag cagcgcatgc tcgatgatct tgtcaaacca gctgcgctcc acgcccatcg
     1441 cggccaggcc ggttggcagg ccgaggcggg cgttcatggc gcgtattgct ttggaggtgg
     1501 cttccggaat gtcgcccgcg tcggccaggc tcatcgcgtg ggccatgcgc tccaggcggc
     1561 gctcggcctt gacactttcg gcttgagcat tgaagcgcac cacggccggc aggaagactg
     1621 cgttcagcgt gccgtggtgc agcctggggt tgatgccgcc caggctgtgg ctgagcgaat
     1681 gcacgcagcc caggcccttc tggaaggcca tcgcgccttg catcgacgcg ctcatcattt
     1741 gcctgcgcgc gtctcggtct tgcccatcgc gcgtggccgc ttcgatatgc gcccagccgc
     1801 gctccaggcc gtccagcgcg atgccgtcgg ccggcgggtt gaacggcggc gccatgaaag
     1861 tctccatgca atgcgcgatc gcgtccatgc cggtggcggc ggtcagcagc ggcggcaggc
     1921 ccagggtcag ctcggggtcg cagatcgcgc tcctgggcac cagatgccag gagtgaaagc
     1981 ccagcttgcg cccgtcgtcg acgatcacga tggcgccgcg cgccacctcg ctgccggtgc
     2041 cggccgtggt gggcacggcg atcaacggcg ccacggcggc ggtgatcttg ggcgagccgc
     2101 cttctatcgt ggcgtaggtc ttgagcgggc cagggtgggt ggccatgatg gccaggccct
     2161 tggccaggtc gatgctggag ccgccgccca cggccaccag gccgtcgcag cggttcgcct
     2221 tccacacctc gtgcgcgctg cgcaccgccg cctcggtggg gttggacggc gtggcatcga
     2281 agacggcgag cggcaagtcc ttgagcgcgt ccagcgccgg ctgcagcacg ccggccgcgc
     2341 gcacaccggc atcggtgacg atcagcgggc gctggatgcc gacgcgctcg cactcggcgg
     2401 cgagctggcg cacggcgccg aagtcgaatt ggacctgggt gatgtagaaa atcgtggaca
     2461 tggcgggcgg ctgaggccgt cgaggcgagc cttgagtatg ctcgcagcct ccactctcag
     2521 cgcaccgcaa atcacagtga gaaccccatg gacagccgcg tgagcgtatt ccccgatctg
     2581 ccgctcacgc cgcagatgcg cggcatcctg agccgcatgc agcgcgcccg cctgccgcgc
     2641 ttctataccc tcacgcccca gca
//
LOCUS       DASUCY010000197         2741 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_148779, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000197 DASUCY010000000
VERSION     DASUCY010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148779"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..118)
                     /locus_tag="VFV25_03800"
     CDS             complement(<1..118)
                     /locus_tag="VFV25_03800"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEY1392470.1"
                     /translation="MEIFDYDNILLLPRKCRVVSRSECDASVEFGGRRFRLPV"
     gene            complement(376..1071)
                     /locus_tag="VFV25_03805"
     CDS             complement(376..1071)
                     /locus_tag="VFV25_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392471.1"
                     /translation="MKKTFGLLGLLAAACALPAQAQIARCGAPAPTLVVERYTSADCE
                     ACWRATPPGDGAKIKGQPFVLDWIVPSARGDEAPLAPAALSEASTRLTRSGALSPDET
                     LSTRMPLPSRSALSLQVEQGPGWYGYMALRMKTSFVSNRPLPSGLAAYLALVESIPAG
                     EEGTPVPRRVVRTLVGPLSLQGLAAGHPVAQLQAARLPQNGKPERLVAVGWVETAAGH
                     VLAVGEACLAPEN"
     gene            1097..>2741
                     /locus_tag="VFV25_03810"
     CDS             1097..>2741
                     /locus_tag="VFV25_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY1392472.1"
                     /translation="MDVHSDEAAISTASGGLLRHLNPEQLAAVTLPRGHALILAGAGS
                     GKTRVLTTRIAWLLQTGQVSPGGVMAVTFTNKAAKEMLTRLSTMLPVNVRGMWIGTFH
                     GLCNRFLRAHWKLANLPQSFQILDSQDQLSAVKRVVKAMNLDEERFVPKQVAWFIAGS
                     KEDGLRPKDIEIRDAQTKTLVEIYQAYEDQCLREGVVDFGELMLRVYELMRDNAALRE
                     HYQRRFRHILVDEFQDTNRLQYAWLKMFAGADNNVFAVGDDDQSIYAFRGAQVGNMAL
                     FEREFHVEQVIKLERNYRSFGNILDAANELIRHNSRRLGKNLRTEAGPGEPVRVFEAT
                     SDFAEAQWFLEEARQLIREGSARSEIAVLYRSNAQSRVIESALFNAGMPYRVYGGLRF
                     FERAEVKHALAYLRLIENPSDDTSFLRVVNFPVRGIGARSIEQLQDAARASGRSLYQS
                     AGALSGRAGANLAAFNALVDAMREATRGLTLREIIEHVVQASGLADFYRTEKEGQERL
                     ENLDELVNAAEAFVTQEGFGKDAVALPVDEQPPGAIALGS"
BASE COUNT          453 a          932 c          945 g          411 t
ORIGIN      
        1 ccaccggcag cctgaagcgg cgcccgccga attccaccga ggcgtcgcat tcgctgcggc
       61 ttaccacgcg gcacttgcgc ggcagcaaga ggatgttgtc gtagtcgaag atttccatga
      121 tgttcaagag ctcctggtgc gagcggcgcg ttgatgcagt gcgcggccca atggctgcga
      181 gcgcttgagg aaacccggtc gaacggccaa agcctggcag gcaggggagc cgtttttgag
      241 atcgcgcggg gcgtgcgagc cccataaacg aaaaaccggg cgccaatctg ggcccggtga
      301 cgtagtctac tccgcttcgc cgcgccgtgc aggcatcagt tttgccgtgc cggcatcagt
      361 tttgccatgc cggcatcagt tttccggcgc caggcaggcc tcgccgaccg ccagcacatg
      421 cccggccgcg gtctcgaccc aacccactgc caccaggcgc tcgggcttgc cgttctgggg
      481 caggcgcgcg gcctgcagtt gcgcgacagg gtgccctgcc gccagcccct gcaggctcag
      541 cggcccgacc agggtgcgca ccacgcgccg cggcaccggc gtgccttcct cgcccgccgg
      601 gatgctctcg accagcgcga gataggccgc gagcccggac ggcagagggc ggttcgacac
      661 gaagctcgtt ttcatgcgca aggccatgta gccataccag cccggcccct gctcgacctg
      721 caggctcagg gcgctgcgcg agggcagcgg catgcgcgtc gacagcgtct cgtcgggcga
      781 tagcgcgccg ctgcgtgtca ggcgtgtgct cgcctcggag agggcggcgg gcgccagcgg
      841 ggcttcgtcg ccgcgcgctg agggcacgat ccagtcgagc acgaagggct ggcccttgat
      901 ctttgcgccg tcgccgggcg gcgtggcgcg ccagcaggcc tcgcagtcgg cggaggtgta
      961 gcgctcgacg accagcgtgg gcgcgggtgc gccgcatctg gcgatctggg cctgggcggg
     1021 caaagcacag gcggcggcga gcaggcccag caggccgaag gtcttcttca tgcggtcgct
     1081 tccctagaat cctcggatgg atgtgcattc tgacgaggcg gcgatctcca ccgcaagcgg
     1141 tgggctgttg cgccacctga accccgaaca actcgccgcc gtgacgctgc cgcgcggcca
     1201 cgcgctgatc ctggccggcg ccggctcggg caagacccgc gtgctgacca cgcgcattgc
     1261 ctggctgctg cagacgggcc aggtctcgcc gggcggcgtg atggccgtga ccttcaccaa
     1321 caaagcggcc aaggagatgc tgacgcggct gtcgaccatg ctgccggtga acgtgcgcgg
     1381 catgtggatc ggcaccttcc acggcctgtg caaccgcttc ctgcgcgcgc actggaaact
     1441 cgcgaacctg ccgcaatcct tccagatcct cgacagccag gaccagctct cggcggtcaa
     1501 gcgcgtcgtc aaggcgatga acctggacga ggagcgcttc gtgcccaagc aggtggcctg
     1561 gttcatcgca ggcagcaaag aagacggcct gcgtcccaag gacatcgaga tccgcgatgc
     1621 gcagaccaag accctggtcg agatctacca ggcctacgag gaccagtgcc tgcgcgaggg
     1681 cgtggtcgat ttcggcgagc tgatgctgcg cgtctacgag ctgatgcgcg acaacgcggc
     1741 gctgcgcgag cattaccagc ggcgcttccg ccacatcctg gtcgacgagt tccaggacac
     1801 caaccgcctg caatacgcct ggctcaagat gttcgcgggc gccgacaaca atgtcttcgc
     1861 ggtaggcgac gacgaccaga gcatctatgc cttccgcggc gcccaggtcg gcaacatggc
     1921 gctgttcgag cgcgagttcc atgtggagca ggtcatcaag ctggagcgca actaccgcag
     1981 cttcggcaac atcctcgatg cggccaacga gctgatccgc cacaacagcc ggcgcctggg
     2041 caagaacctg cgcaccgagg ccggccccgg cgagccggtg cgcgtcttcg aggcgaccag
     2101 cgacttcgcc gaggcgcagt ggttcctgga agaggcgcgc cagctgatcc gcgagggttc
     2161 ggcgcgcagc gagatcgcgg tgctctaccg cagcaacgcg cagtcgcgcg tgatcgaatc
     2221 ggcgctcttc aacgccggca tgccctaccg cgtctacggc ggcctgcgct tcttcgagcg
     2281 cgccgaggtc aagcatgcac tggcctatct gcgcctgatc gagaacccga gcgacgacac
     2341 cagcttcctg cgcgtcgtga acttcccggt gcgcggcatc ggcgcgcgct cgatcgagca
     2401 gctgcaggac gcggcgcgcg ccagtgggcg cagcctctac cagagcgcgg gcgccttgag
     2461 cggccgcgcg ggcgccaatc tcgccgcctt caatgcgctg gtcgatgcga tgcgcgaagc
     2521 gacgcgaggc ctgacgctgc gcgagatcat cgagcatgtg gtgcaggcct cgggcctggc
     2581 cgacttctat cgcaccgaga aggagggcca ggagcggctg gagaacctgg acgaactggt
     2641 caacgcggct gaggccttcg tcacccagga gggcttcggc aaggacgccg tggcgctgcc
     2701 ggtggacgag cagccgcccg gcgcgatcgc gctcggcagc c
//
LOCUS       DASUCY010000198        10592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1417817, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000198 DASUCY010000000
VERSION     DASUCY010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 10592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10592
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1417817"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1576
                     /locus_tag="VFV25_03815"
     CDS             <1..1576
                     /locus_tag="VFV25_03815"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEY1392473.1"
                     /translation="TQFGQHVLGDEAAWQLALNGEADLAGLPEWVRNAAREAAAQRGS
                     DGHMVSLSRSLVMPFLTFSRRRDLREAVWRAWTSRGEATPERDTRPIAVEIMQLRLEQ
                     AKLHGYSSYADYALRDRMAGKPQAVSELLQRVWGRAKARAEEERKLLEAQAAAGGADG
                     RGEGAIALQPWDWRYYAEQVRAARYQLDDAEIKPYFQLDRMIDAMFDCAQRLFGLRFI
                     EQRALALYHPDVRAWEVRDAKSDVLVGLFLGDNFARPSKRGGAWMSLYRQQSRNTADG
                     AAVLPIVVNNNNFAKGPAGQPTLLSFDDVRTLFHEFGHGLHGLLSDVTYERLSGTNVL
                     RDFVELPSQIYEHWAKEPEVLKRHALHVATGEPIPDALIERLQRARFFNQGYETVQYV
                     GAALIDLALHSRTNMDGLDLDSFEREQRAALGVPEVVGVMHRLPHFRHLFASDSYAAG
                     YYVYMWAEVLDCDGYEAFTEAGNVFDPATAERLKRCIYSAGNSVEPGAAYRAFRGRDA
                     KVDAMLADRGLVGELV"
     gene            complement(1655..2971)
                     /gene="hemL"
                     /locus_tag="VFV25_03820"
     CDS             complement(1655..2971)
                     /gene="hemL"
                     /locus_tag="VFV25_03820"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004532260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEY1392474.1"
                     /translation="MSSSSSSSRNQELFERAQRVIPGGVNSPVRAFRAVGGTPRFITH
                     AEGAYLWDADGKRYIDYIGSWGPMILGHGHPAVLEAVSKAAAEGFSFGAPTEREIELA
                     EEILKLVPSAEQVRLVSSGTEAAMSAIRLARGATGRAKLIKFEGCYHGHADALLVKAG
                     SGLATFGHPTSAGVPPEVVQHTVVLEYNNLAQLEAAFAQHGEELACVMIEPIAGNMNF
                     VRAAVPFMKRLRELCSQHGALLVFDEVMTGFRVALGGAQSLYAKAIPGFKPDLSVFGK
                     VIGGGMPLAAFAGPRALMHQLAPLGPVYQAGTLSGNPVATACGLATLKEIQKPGFFNA
                     LARSTRTLVDGLAAAGREAGVPLVGDCEGGMFGFFFAHSFDDALPQNYGEVMATDKER
                     FNRFFHGMLERGVYLAPALYEAGFVSAAHSPADIEATLQAARAALR"
     gene            complement(3028..4005)
                     /locus_tag="VFV25_03825"
     CDS             complement(3028..4005)
                     /locus_tag="VFV25_03825"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEY1392475.1"
                     /translation="MSPTPSGAEADAELPQDQQEPAAPACVMSFNASDPSGAGGLAGD
                     VTTIAAMGAHALPVVTAIVLRDTAEVFESSEIEADTIVEQARSILEDVTISAWKVGFL
                     GSAEGVSAVAEVLSDYPDVPLVSYLPNLSWLDEDPLSAYLDAFRELVLPQTEVLVGNH
                     KTLTDCLLPEWDSERAPSARELAVAANQHGARYVLVTGVLLPGKGNDQFIDNVLASPQ
                     GAITGEKFERFETAFVGAGETLSAALAALLASGQELHVAVSEALSFLDQSLDAGFRPG
                     MGNVVPDRFFWALPPAEEGEAGGEEPPEGGGSAGGAGGGGADSPTRSIH"
     gene            4183..4335
                     /locus_tag="VFV25_03830"
     CDS             4183..4335
                     /locus_tag="VFV25_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEY1392476.1"
                     /translation="MCLICGWIYDEEAGDPEHGIAAGTKWSDVPMNWTCPECGARKED
                     FEMVQI"
     gene            4395..4799
                     /locus_tag="VFV25_03835"
     CDS             4395..4799
                     /locus_tag="VFV25_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY1392477.1"
                     /translation="MSEAVRPGSAAATKVLVIDDSNTIRRSAEIFLKQGGYEVVLAED
                     GFDALAKVNDLQPHLIFCDILMPRLDGYQTCAIIKRNPRFAEVPVIMLSSKDGLFDKA
                     RGRMVGSEDYLTKPFTKDQLLQAVEQHRPAIA"
     gene            4898..5263
                     /locus_tag="VFV25_03840"
     CDS             4898..5263
                     /locus_tag="VFV25_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY1392478.1"
                     /translation="MPINKILLVDDSKTELHFLSELLVKRGYQVRTAENGDEAMRRLA
                     EDKPDLILMDVVMPGLNGFQLTRAITRDPNYGNIPVIMCTSKNQETDKVWGMRQGASD
                     YVVKPVDADELIAKIKAFA"
     gene            5299..5826
                     /locus_tag="VFV25_03845"
     CDS             5299..5826
                     /locus_tag="VFV25_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEY1392479.1"
                     /translation="MAKREALRELQSRLAERLQASRTQGASATWLAVESGGQGFLLPL
                     GEAGEIFSLSALVPVPHTSGWFLGVANLRGGLHGVVDLAGFLGIKSQAANEPGREQSR
                     LVAFNPSLEINCAMLVDRLAGLRSREQLEAHAEDGGPKPGFVGGRYRDEQGRSWQELR
                     LAALAGDEAFLKIVG"
     gene            5903..8242
                     /locus_tag="VFV25_03850"
     CDS             5903..8242
                     /locus_tag="VFV25_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY1392480.1"
                     /translation="MSFLSKIKGFGRPKSASDAADDLPFDEAYPVADDFNVHAGGTAA
                     MPTIALEGSTMQHTVAADSSIITEAAASEMGAGEFSETRLQGGEAGAAASGARLPFIG
                     HLQTGRQQRILGGMLALGVLGLIASSIWSVNTANKSAAQVGASGQALMQSQRLAKSVS
                     QALIGSPQAFPEVKESIDVLTRNVRGLKNGNATLDAAPGAVQSTLDPLQPLVDRAEKN
                     AHTVLDQQKVLTQVGQALRSINRQSSDLLEIAETVSSLKLQQDASPAELSAVGQLVML
                     TQRIGKSANEFLTMEGVSPEAVFLLGKDLNSFREITQGLIDGNPELRLPATKDAQTKE
                     RLQALMQLYEETRSQAGTILGNLQGLVSAREAQSAIIADSEPLRKGLEQVQTELTGGS
                     GLSGLNLALIVLSLLLAVAGAAGLLMAYLQDQRQRAAVAEKQRLEAERQEQEAKRVND
                     ANQAAILRLMNELQTVAEGDLTQQATVTEDITGAIADSVNYTVEELRSLVSQVQGTVS
                     RVTETTQQVEATSTELLAASDEQLREIRETGESVLQMAGRINDVSAQAQQSAQVARQS
                     LQAAESGLHAVQNTIGGMNTLREQIQETSKRIKRLGESSQEIGEITELISDITEQTNV
                     LALNAAIQAASAGDAGRGFSVVAQEVQRLAERSGDATKQIAALVKTIQTDTADAVAAM
                     ERSTQGVVEGAKLSDNAGTALGEIDRVSRQLAELITSISSQASREAQSANVVAANIQH
                     IFAVTEQTGEGTRSTAQLVRELSKTAEELKQSVARFKIG"
     gene            8290..>10592
                     /locus_tag="VFV25_03855"
     CDS             8290..>10592
                     /locus_tag="VFV25_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEY1392481.1"
                     /translation="MESHRDSEMIDDLSSLAWVQDELRKSLDTAHKALRRSLKEMEAA
                     AGSDVDAVDPAILRGARAQIHQGVGALELVGLPAGAALLRGSEGAVQRYVAKPHKLDL
                     AAVEAIERASFALLDYVARLLAGKPASPVALFPQYRAVQELAGAERIHPADLWPYDWA
                     WRDIPGDGSAPVPANAAAVAGFEQLLLAQMRKPTPQSAQQMSDLCAGLANGARDHEAA
                     TLWRLAAAFFEAQSHGLLEPDIHGKRVASRLLAQLRALIKGEAEVAERLAQDLLFFCA
                     QTVEADAGAAPRLHAARTAYELAGHAPVDYSQAMLGRFDPAWIAQARKRVATAKDSWS
                     AVAGGELHRLSGLNEQYALVGDSLKRLYSTGEPLAEALQRAVAQTVQSAASPAPSLAM
                     EVATSVLYLEASLEDGDFDHAEQASRIARLAERIEAVRTGGQPAPLESWMEELYRRVS
                     DRQTMGSVVQELRSTLSEAEKQIDQYFRNPADRTVLIPVPGQLQAMRGVLSVLDMGQA
                     AQAVLRMRDDVDALVITEVDTERAAASGTFDRLAGNLSALSFLIDMLSVQPQMAKSLF
                     VFDPASGRLNPVMGRSRSDQVTLPESKVAAAPHLIEQAQSLAEAVKHKNVPLAQVSRE
                     LDRLSQEALVSDQPALAASVSSAQAALHQAKQAGAGRATLEAARGQVARALADFVVTA
                     SGPIGLDAMASAPAPLVLPPLMPSAPAPLAATGLEEDDEMRDIFLEEAREVMGNADGA
                     LAVLDSDPGDAGEITVLRRAFHTLKGSS"
BASE COUNT         1778 a         3562 c         3649 g         1603 t
ORIGIN      
        1 cacgcaattc ggccagcatg tgctgggcga cgaggccgcc tggcagctgg cgctgaacgg
       61 cgaagccgac ctggcgggcc tgcccgagtg ggtgcgcaat gcggcacgcg aggcggccgc
      121 gcagcgcggc agcgacggcc acatggtgtc gctgtcgcgc tcgctcgtga tgcctttcct
      181 cacctttagc cggcggcgcg acctgcgcga agcggtgtgg cgcgcctgga cctcgcgcgg
      241 cgaagccacg cccgagcgcg acacgcgccc catcgccgtc gagatcatgc agctgcgcct
      301 ggagcaggcc aagctgcacg gctactcgag ctatgccgac tacgcgctgc gagatcgcat
      361 ggccggcaag ccgcaggcgg tgagcgagtt gctgcagcgc gtgtggggga gggccaaggc
      421 gcgtgctgag gaggagcgca agctgctcga agcccaggcc gccgccggcg gcgctgatgg
      481 tcggggtgag ggcgcgatcg cgctgcagcc ctgggactgg cgctactacg ccgagcaggt
      541 gcgtgcggcg cgctaccagc tcgacgatgc cgagatcaag ccctacttcc agctcgaccg
      601 catgatcgat gcgatgttcg attgcgccca gcgcctgttc ggcctgcgct tcatcgagca
      661 gcgcgctctc gcgctctacc accccgacgt gcgggcctgg gaggtgcgcg acgcgaagag
      721 cgatgtgctg gtgggcctct ttctgggcga caacttcgcg cggcccagca agcgcggcgg
      781 cgcctggatg agcctgtacc gccagcagtc gcgcaacacg gccgacgggg ccgcagtgct
      841 gcccatcgtc gtcaacaaca acaactttgc gaagggcccg gccggccagc ccaccctgct
      901 cagcttcgac gacgtgcgca cgctgttcca cgagttcggc catggcctgc acggcctgct
      961 gagcgacgtg acctacgagc gcctgtcggg caccaacgtg ctgcgcgact tcgtcgagct
     1021 gccctcgcag atctacgagc attgggcgaa ggagcccgag gtgctgaagc gtcacgcgct
     1081 gcacgtcgca accggcgaac ccatccccga tgcgctgatc gagcggctgc agcgtgcgcg
     1141 cttcttcaac caaggctatg agacggtgca atacgtcggc gccgcgctga tcgacctcgc
     1201 gctgcacagc cgcacgaaca tggacgggct cgacctcgac agcttcgagc gcgagcagcg
     1261 cgcggcgctg ggcgtgcccg aggtggtggg cgtgatgcac cgcctgccgc atttccgcca
     1321 cctgttcgcg agcgacagct atgcggccgg ctactacgtc tacatgtggg ccgaggtgct
     1381 cgactgcgac ggctacgagg ccttcaccga ggccggcaac gtgttcgacc ccgccaccgc
     1441 cgagcggctc aagcgctgca tctacagcgc cggcaacagc gtcgagccgg gcgcggccta
     1501 ccgcgccttc cgcgggcgcg atgccaaggt ggatgcgatg ctggccgatc gcggcctagt
     1561 tggcgagctg gtgtgagtgc taggttgaag aaaaatcgcc ggataagtgg aagcaatcag
     1621 gcgatttttg gtccaaaaac tgtagcgttg ccgatcagcg cagtgcggcg cgcgccgcct
     1681 gcagcgtggc ctcgatatcg gccgggctgt gcgccgcgct gacgaagccg gcctcgtaga
     1741 gcgccggcgc gaggtagacg ccgcgctcca gcatgccgtg gaagaagcgg ttgaagcgct
     1801 ccttgtcggt cgccatcacc tcgccgtagt tctgcggcaa tgcgtcgtcg aacgaatggg
     1861 cgaagaagaa gccgaacatg ccgccttcgc aatcgcccac cagcggcacg ccggcctcgc
     1921 ggccggccgc tgccaggccg tcgaccagcg tacgcgtgct gcgcgccagc gcattgaaga
     1981 agccgggctt ctggatttcc ttcagcgtcg ccaggccgca ggcggtggca accgggttgc
     2041 cgctcagcgt gccggcctgg tagacggggc cgagcggcgc gagctgatgc atcaacgcac
     2101 gtgggccggc gaaggccgcc agcggcatgc cgccgccgat caccttgccg aacacgctca
     2161 ggtcgggctt gaagccgggg atggccttcg catataggct ctgcgcgccg cccaaagcga
     2221 cgcggaagcc cgtcatcacc tcgtcgaaca ccagcagcgc gccatgctgg ctgcacagct
     2281 cgcgcagccg cttcatgaag ggcacggcag cgcgcacgaa gttcatgttg ccggcgatcg
     2341 gctcgatcat cacgcaggcc agctcctcgc cgtgctgagc gaaggccgct tcgagctgag
     2401 cgaggttgtt gtattcgagc acgacggtgt gctgcaccac ctcgggcggc acgccggcgc
     2461 tggtggggtg gccgaaggtg gccaggcccg agccggcctt cacgagcagc gcgtcggcgt
     2521 ggccgtggta gcagccctcg aacttgatca gcttggcccg gccggtggcg ccgcgcgcca
     2581 ggcggatcgc gctcatcgcc gcctcggtgc ccgagctcac gaggcgcacc tgctcggcgc
     2641 tgggcacgag cttcaggatc tcctcggcca gctcgatctc gcgctcggtg ggcgcgccga
     2701 aggagaagcc ctcggccgcg gccttgctca ccgcctcgag cacggccggg tggccgtggc
     2761 ccaggatcat cgggccccag gagccgatgt agtcgatgta gcgcttgccg tcggcgtccc
     2821 acaggtaggc gccttctgca tgggtgatga agcgcggcgt gccgccgacc gcgcggaagg
     2881 cgcgcaccgg ggaattcacg ccgccgggga tgacgcgctg ggcgcgctcg aagagttcct
     2941 ggttgcgact gctactgctg ctgctgctca tgctgcgccg ttggctgtgt tctcttttct
     3001 tttgtcgatc aatggatgct gcgcggatca atggatgctg cgcgttgggc tgtccgcgcc
     3061 gcctccgcca gcaccgcctg ccgatccgcc gccctcaggg ggctcctcgc cgcccgcttc
     3121 gccttcctcg gccggcggca gggcccagaa gaagcggtcg ggcaccacat tgcccatgcc
     3181 ggggcggaag cctgcatcca gcgactggtc gaggaagctc aaggcttccg ataccgcgac
     3241 gtgcagctcc tggccactgg ccagcagcgc ggcgagcgcg gccgacagcg tttcgccggc
     3301 gccgacgaag gcggtctcga agcgctcgaa cttctcgccg gtgatcgcgc cctggggcga
     3361 ggcgagcacg ttgtcgatga actggtcgtt gcccttgccg ggcagcagca cgccggtcac
     3421 cagcacatag cgcgcgccat gctggttggc ggccacggcc agctcgcgcg ccgagggcgc
     3481 ccgctcggag tcccattcgg gcagcaggca gtcggtcagg gtcttgtggt tgcccaccag
     3541 cacctcggtc tgcggcagca ccagttcgcg gaaggcgtcg agataggccg agagcgggtc
     3601 ctcgtccagc caggacaggt tgggcaggta ggacacgagc ggcacatcgg ggtagtcgct
     3661 cagcacctcg gccacggcgc tcacgccttc cgcgctgccc aggaagccga ccttccaggc
     3721 cgaaatggtc acgtcctcga gaatgctgcg cgcctgctcg acgatggtgt cggcctcgat
     3781 ctcggaagac tcgaagacct cggccgtgtc gcgcagcacg atggccgtga ccacgggcag
     3841 cgcgtgcgcg cccatcgcgg cgatggtcgt cacatcgccc gcgaggccgc cggcgccgct
     3901 cgggtcgctg gcgttgaagc tcatcacgca ggcgggtgcc gccggctcct gctggtcctg
     3961 cgggagttcg gcatcggctt cggcgccgct gggggtaggg ctcatcgggt cagtcagggg
     4021 ctgcaggccg ggctcgcggc gggcgcgtcc gacctctaga aaaatgtgag gaatgttgcg
     4081 tatgtgccca ggaaccctag tatttcctct ggttcgcgtg gctacaatcg tcactcattg
     4141 tagttaagcc acaaagataa cgtgagcgaa tcgcgaacct ggatgtgcct catttgcgga
     4201 tggatctatg acgaggaggc gggggacccg gaacatggca tagcggccgg caccaagtgg
     4261 tcggatgtac ccatgaactg gacctgcccc gagtgcggcg cccgcaagga ggacttcgag
     4321 atggtgcaga tctagccacg acgcttttcc ccagggctct ttccccatgg gtctcagtcg
     4381 ttgaggagtt atccgtgagc gaagcagtgc ggcccggttc ggcggcagcc accaaggtgc
     4441 tggtcataga cgacagcaat accatcaggc gcagcgccga gatcttcctg aagcaggggg
     4501 gctacgaggt cgtcttggcc gaagacggct tcgacgccct ggccaaggtc aatgacctgc
     4561 agccccacct gatcttctgc gacatcctga tgccgcgcct ggacggctac cagacctgcg
     4621 ccatcatcaa gcgcaatccc agattcgccg aggtgccggt catcatgctg tcctccaagg
     4681 acggcctgtt cgacaaggcg cgcggtcgca tggtgggctc ggaagactat ctgaccaagc
     4741 ccttcacgaa agatcaactg ctgcaggccg tggagcagca tcgccctgcg attgcctgag
     4801 gctgcacaac ccgtgccggg cgtggaatac cgcacagaaa ttccagcgat acccggcttg
     4861 cgccgcccga tgattcgaaa ctttaggaaa tagagccatg cctatcaaca aaatcctgct
     4921 ggtggacgac tccaagaccg agctccactt cctgtccgaa ctgctggtca agcgcggcta
     4981 tcaggtgcgc accgccgaga acggcgacga ggccatgcgc cgcctggccg aggacaagcc
     5041 cgacctgatc ctgatggacg tcgtgatgcc cggcctcaac ggcttccagc tcacgcgcgc
     5101 catcacccgc gatcccaact acggcaacat cccggtgatc atgtgcacca gcaagaacca
     5161 ggagaccgac aaggtctggg gcatgcgcca gggtgcgagc gactatgtcg tcaagccggt
     5221 ggacgccgac gagctgatcg ccaagatcaa ggccttcgcc tgaacgcagg cccgggacaa
     5281 agcagcaagc agggatgaat ggctaagaga gaagcactgc gcgaattgca aagccggctt
     5341 gccgagcgcc tgcaggcctc gcgcacgcag ggcgccagtg ccacctggct ggcggtcgaa
     5401 tcgggcggcc agggctttct gctgccgctg ggcgaagccg gcgagatctt ctcgctgagc
     5461 gcgctggtgc cggtgccgca cacgagcggc tggttcctcg gcgttgccaa cctgcgcggc
     5521 ggcctgcacg gtgtggtcga cctggccggc ttcctgggca tcaagtctca agcggccaat
     5581 gagccggggc gcgaacagtc gcgcctggtg gccttcaatc cgagcctgga gatcaactgc
     5641 gcgatgctgg tggaccggct tgccggcctg cgcagccgcg aacagctcga ggcccatgcc
     5701 gaggacggcg ggcccaagcc cggcttcgtg ggcggccgct accgcgacga gcagggccgc
     5761 agctggcagg aactgcgcct ggcggcgctg gccggcgacg aggcatttct gaagatagtg
     5821 ggctgagtcg cagtgtcgat agtcagccgg gtttccaaag agcagtagtc agcagcagac
     5881 gcgcaagcaa gaagaggggc ctatgagttt tctgagcaag atcaagggtt tcggcaggcc
     5941 caagtcggcg tccgacgcgg cggacgatct gcccttcgac gaggcctatc cggtggcgga
     6001 cgatttcaac gtccatgccg gcgggactgc tgccatgccg actatcgcgc ttgagggatc
     6061 cacgatgcaa cacacggtgg ccgccgattc ctccatcatc accgaggcgg ccgcgtccga
     6121 gatgggcgct ggcgagttca gcgaaacgcg cctgcagggc ggcgaagccg gggctgcggc
     6181 ctccggcgcg agactgcctt tcatcggcca tctgcagacc gggcggcagc agcgcattct
     6241 cggcggcatg ctggcgctgg gcgtgctggg cctgatcgct tcgtcgatct ggtcggtgaa
     6301 cacggccaac aagagcgcgg cccaggtggg tgccagcggc caggccctga tgcagtcgca
     6361 gcgactggcc aagtcggtgt cgcaggcgct gatcggcagc ccccaggcct tccccgaggt
     6421 caaggaaagc atcgacgtgc tgacgcgcaa cgtgcgcggc ctgaagaacg gcaacgccac
     6481 tctcgatgcg gcgcccggtg cggtgcagtc gacgctggat ccgctgcagc cgctggtgga
     6541 ccgcgccgag aagaacgccc acaccgtgct ggatcagcag aaggtgctga cccaggtcgg
     6601 acaggccctg cgctcgatca accgccagtc ctccgacctg ctcgaaatcg ccgagaccgt
     6661 gtcctcgctg aagctgcagc aggacgcctc gcccgccgag ctttccgccg tcggccagct
     6721 cgtgatgctg acccagcgca tcggcaagtc ggccaacgag ttcctgacca tggagggcgt
     6781 ctcgcccgag gctgtgttcc tgctgggcaa ggacctgaac tccttccgcg aaatcacgca
     6841 gggcctgatc gacggcaacc cggagctgcg cctgcctgcc accaaggacg ctcagaccaa
     6901 ggagcggctg caggcgctga tgcagctcta tgaagagaca cggtcccagg ccggcacgat
     6961 tctcggcaac ctgcaaggcc tggtgtcggc ccgtgaagcg cagtcggcca tcattgccga
     7021 cagcgaaccg ctgcgcaaag gcctggagca ggtgcagacc gagctgaccg gcggctccgg
     7081 cctctccggt ctcaatctgg cgttgatcgt gctgtcgctg ctgctggcgg tggccggcgc
     7141 cgcgggcctc ttgatggcct atctgcaaga ccagcgccag cgcgcggcgg ttgccgaaaa
     7201 gcagcggctg gaagccgaac gccaggagca ggaggccaag cgcgtcaacg acgcgaacca
     7261 ggccgccatt ctgcgtttga tgaacgagct gcagacggtg gccgagggcg acctgacaca
     7321 gcaagccacg gtgaccgagg acatcaccgg cgccatcgcc gactcggtga attacacggt
     7381 ggaagagctg cgctccctgg tgtcgcaggt gcagggcaca gtgtcgcgcg tgaccgaaac
     7441 gacccagcag gtggaggcga cgtcgaccga gctgctggcc gcttccgacg agcagctgcg
     7501 cgagattcgc gaaaccggcg aatcggtgct gcagatggcg ggccgaatca acgacgtgtc
     7561 ggcgcaggcc cagcagtcag cccaggtggc gcgccagtcg ctgcaggctg ccgagtccgg
     7621 cctgcacgcg gtgcagaaca ccatcggcgg catgaacacg ctgcgcgagc agatccagga
     7681 aacctccaag cggatcaagc ggctgggcga atcctcgcag gagatcggcg aaatcaccga
     7741 gctgatttcc gacattaccg agcagaccaa cgtgctggcg ctgaacgccg ccatccaggc
     7801 cgcctcggcc ggcgacgcag gccgcggctt ctcggtggtg gcgcaggaag tgcagcgcct
     7861 ggccgaacgc tcgggcgatg cgaccaagca gatcgcggcg ctggtgaaga ccattcagac
     7921 cgacacggcc gacgccgtgg ccgctatgga acgttccacc cagggcgtgg tcgagggcgc
     7981 caagctgtcc gacaacgcag gtacggcgct gggcgagatc gaccgcgtgt cgcgccagct
     8041 cgccgagctg atcacgagca tctcttccca ggcctcgcgc gaagcgcagt ccgccaacgt
     8101 cgtggcggcc aacatccagc acatcttcgc cgtgaccgag cagaccggcg agggcacgcg
     8161 ctcgacggcg cagctggtgc gcgagctgtc caagacggcc gaggaactga agcagtcggt
     8221 cgctcgattc aagatcggtt gaggtccggg cgcgccagcg tgccgggcga tacagataca
     8281 aggtgacgga tggaaagcca ccgcgactcc gagatgatcg acgacctcag ctccctggcc
     8341 tgggtgcagg acgagttgcg caagtcgctc gataccgccc acaaggcgct caggcgcagc
     8401 ctgaaggaga tggaggcggc ggccggctcc gatgtcgacg cggtcgatcc ggccatcctg
     8461 cgcggcgcgc gggcgcagat ccatcagggc gtgggcgccc tggagctggt cgggctgccg
     8521 gccggcgccg cgctgctgcg cggctccgaa ggggcggtgc agcgctatgt cgccaagccg
     8581 cacaagctcg atctcgccgc ggtggaagcc atcgagcgtg cttccttcgc cctgctcgac
     8641 tatgtcgcgc ggctgctcgc gggcaagccg gcatcgccgg tcgcgctgtt cccgcagtac
     8701 cgcgccgtgc aggagctggc cggcgcagag cgcatccatc ccgccgatct ctggccttat
     8761 gactgggcct ggcgcgacat cccgggcgac ggctccgcgc cggtgccggc caatgcggcc
     8821 gccgtagcgg gcttcgaaca gctgctgctg gcgcagatgc gcaagccgac gccgcagtcc
     8881 gcccagcaga tgagcgatct ttgcgcgggc ctggccaatg gcgcgcgcga ccacgaggcc
     8941 gccacgctgt ggcgcctggc tgcggccttc ttcgaggcgc aaagccacgg tctgctggaa
     9001 cccgatattc atggcaagcg ggtcgcctcg cggctcctgg cccagctgcg cgcgcttatc
     9061 aagggcgagg ccgaggtggc cgagcgcctg gcgcaggacc tgctgttctt ctgcgcgcaa
     9121 accgtcgaag ccgatgcggg cgccgcgccg cgtctgcatg ccgcccgcac tgcctatgag
     9181 ctcgcaggcc atgcgccggt cgactacagc caggcgatgc tgggccgctt cgatccggcc
     9241 tggatcgcgc aggcgcgcaa gcgcgtggcc actgccaagg attcctggtc ggcagtggcc
     9301 ggcggagagc tgcatcgcct gtcggggctg aacgagcaat atgccctggt cggcgactcg
     9361 ctgaagcgcc tctacagcac tggcgaaccg ctggcggagg ctttgcagcg cgccgtcgcg
     9421 cagaccgtgc agagcgccgc cagccccgcg ccttcgctgg cgatggaagt ggccaccagc
     9481 gtgctctatc tcgaggcctc gctggaagat ggcgacttcg accatgccga gcaagcctcg
     9541 cgcatcgcgc ggctggcaga gcgcatcgag gcggtgcgca ccggcggcca gccggcgccg
     9601 ctggagtcct ggatggagga gctctaccgc cgcgtgtccg accgccagac catgggcagc
     9661 gtcgtgcagg agctgcgcag cacgctgtcc gaggccgaga agcagatcga ccagtacttc
     9721 cgcaacccgg ccgaccgcac cgtgctgatc ccggtgccgg gccagctgca ggccatgcgc
     9781 ggcgtgctgt ccgtgctgga catgggccag gccgcccagg ccgtgctgcg catgcgcgac
     9841 gatgtcgatg ccctcgtcat caccgaggtc gacaccgagc gcgccgccgc gtccggcacc
     9901 ttcgaccgcc tcgccggcaa cctgagcgcc ctgagcttcc tgatcgacat gctcagcgtg
     9961 cagcctcaga tggcgaagtc gctgtttgtc ttcgatccgg cgagcggacg actgaatccc
    10021 gtgatgggcc gcagccgcag cgaccaagtc accttgcccg aatcgaaggt cgccgccgcg
    10081 ccgcatctga tcgagcaggc ccagtcgctg gccgaggcgg tcaagcacaa gaacgtaccg
    10141 ctggcccagg tctcgcgcga actcgatcgc ttgtcgcaag aagccctggt gagcgatcag
    10201 cctgcgctgg ccgccagcgt ttccagcgcg caggccgcgc tgcatcaggc caagcaggcc
    10261 ggcgccggcc gcgcgaccct ggaggccgcg cggggccagg tcgcccgggc cctggccgac
    10321 ttcgtcgtca ccgcctccgg gccgatcggc ctggacgcca tggcctctgc gccggcgccg
    10381 ctggttctcc cgccgctgat gccgagtgcg cctgcgccgc tggcggcgac cggcctcgaa
    10441 gaagacgacg agatgcgcga catcttcctg gaggaagcgc gcgaagtcat gggcaatgcc
    10501 gacggggcgc tggcggtcct ggacagcgat cccggcgatg cgggcgagat cacggtgctg
    10561 cgccgcgcct tccacacgct caagggcagc tc
//
LOCUS       DASUCY010000199         6186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1417832, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000199 DASUCY010000000
VERSION     DASUCY010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 6186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6186
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1417832"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VFV25_03860"
     CDS             complement(<1..278)
                     /locus_tag="VFV25_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HEY1392482.1"
                     /translation="MNAPLPLTQIAGQTEEDAAPRLREIPYNYTSFSDREIVIRLLGE
                     RAWEVLNRLREERRTGRSARMLYEVLGDIWVVQRNPYLQDDLLDNAKRR"
     gene            531..1844
                     /gene="ugpB"
                     /locus_tag="VFV25_03865"
     CDS             531..1844
                     /gene="ugpB"
                     /locus_tag="VFV25_03865"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HEY1392483.1"
                     /translation="MKLKFASLAGAAFLALASVPAQAVTEITWWHSMEQALGERVNEI
                     ARQFNASQSEYKVVPVFKGNYAESMAAGIAAYRGGNAPDIIQVFEVGTATMMSAKGVI
                     KPVEELMRENGEKFDRNAYIPAVAGYYTSAKGEMLSFPFNSSTTVMYYNKDAFAKAGL
                     DANKPPVTWQELVADAAKLKASGAKCAYTTAWQAWIQLESFSTLHNVEFATKNNGFGG
                     GDARLVFNSPLHVRHIEDMGNWAKQGYFTYAGRKDEGNASFFSGDCAILTASSGAYGN
                     IAKNAKFKFGVAPLPYYSDVSGAPQNPVIGGASLWVMGGKSKAEYKGVAKFLSFLSQP
                     ELQAKWHQETGYLPITTAAYELTKKSGFYEQKPGTDTAVKAMIVKTTAKSRGIRLGNM
                     AQIREVIDQELENVWNGKKTAKEALDEAVRRGNELLEKFEKANKS"
     gene            1892..2773
                     /gene="ugpA"
                     /locus_tag="VFV25_03870"
     CDS             1892..2773
                     /gene="ugpA"
                     /locus_tag="VFV25_03870"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HEY1392484.1"
                     /translation="MEKRARFSSAWLPYALVAPQIAVTLVFFFWPAAQALWQSTLLQD
                     AFGVQTEFVGLDNFVALWNDPHYLDAFKTTAMFSLLVAVSGMAVALLLAVFADRVLRG
                     ARWYRTLLIWPYAVSSVVAGVLWMFLFSPSLGLLTHALAWFGMTWNHNVDSRQAMALI
                     VIAATWKQISYNFLFFLAGLQSIPKSLIEAAAIDGAGPARRFRDIVFPLLSPTSFFLL
                     VINIVYAFFDTFAIIDITTHGGPGTDTVILVYKVYMDGFKGQDFGGSAAQSVILMLLV
                     ITLTVLQFRFVEKKVQY"
     gene            2775..3626
                     /gene="ugpE"
                     /locus_tag="VFV25_03875"
     CDS             2775..3626
                     /gene="ugpE"
                     /locus_tag="VFV25_03875"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HEY1392485.1"
                     /translation="MVERRPWLTLLSHLVLIAGVLVVVFPIYVTFVASTQNADQVRQV
                     PLSLLPGSEALRNYRTVLFGDADTTGVSPMGHMLWVTLVMALSIAVGKIAISLLSAFA
                     IVYFRFPGRSLFFWMIFVTLMLPVEVRISPTYKIVSDLGLLNSYAGLTLPLIASATAT
                     FLFRQFFLTVPDELAEAARVDGAGPLRFMRDVLLPLSLTNIAALFVIQFIYGWNQYLW
                     PLLVTNNEDMYPIVLGLKRSIGAGGDAAIEWQLVMATAMLAMLPPVLVVMLMQKWFVK
                     GLVDAEK"
     gene            3654..4730
                     /locus_tag="VFV25_03880"
     CDS             3654..4730
                     /locus_tag="VFV25_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
                     /protein_id="HEY1392486.1"
                     /translation="MASISLRSVVKRYGHGPKAPQVIHGVNAEIADGEFIVIVGPSGC
                     GKSTLLRMVAGLEEISDGEIAIGPRVVNELEPAERDIAMVFQNYALYPHMSVYDNMAY
                     GLKIAKVPAEEIKVRVDKAAKILELSQLLERKPRQLSGGQRQRVAMGRAIVRQPQVFL
                     FDEPLSNLDAKLRAQTRLEIQKLHAELGVTSLFVTHDQVEAMTLAQRMIVMNAGRMEQ
                     IGTPEEVYQRPATTFVAGFIGSPPMNLLLGQAQDSQFAIAGARLELPAAAPRAGELIL
                     GVRPEHLRLAEQGWPVKVEMMEMLGAERLVYGRLGGSPDHGLLTLRMDATEAAPGVGQ
                     TVHLAASPERLHWFDARTQERVER"
     gene            4840..5604
                     /gene="ugpQ"
                     /locus_tag="VFV25_03885"
     CDS             4840..5604
                     /gene="ugpQ"
                     /locus_tag="VFV25_03885"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEY1392487.1"
                     /translation="MSPVSFDAWPYPLWIAHRGAGKLAPENTLAAFRLGAAHGYRMFE
                     CDAKLSADGQPFLLHDSTLSRTTNARGPASALSWDELSRLDAGGWHSRAYTGEPLPSL
                     QAIARYVKRNGFALNIEIKPSPGSERHTGRVVAEAAAELWRGEALPPLLTSFKPEALQ
                     GASEAAPHLPRGLLLDALHGDWVGQAQTLGCVAIVSHYGLIDAAMVARVRAARMKLLS
                     YTVNDPAEAYRLHGLGVAGLITDAVDRFTPGPGVQD"
     gene            5640..5716
                     /locus_tag="VFV25_03890"
     tRNA            5640..5716
                     /locus_tag="VFV25_03890"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5674..5676,aa:Met,seq:cat)
     gene            5995..>6186
                     /locus_tag="VFV25_03895"
     CDS             5995..>6186
                     /locus_tag="VFV25_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF817 domain-containing protein"
                     /protein_id="HEY1392488.1"
                     /translation="MLRHVDAALLGASHSSPLARSLPRPLAELAFFGLKQARACLFAA
                     VIFLSVFIVPRGGLWHIPRY"
BASE COUNT         1062 a         2089 c         1965 g         1070 t
ORIGIN      
        1 cgccgcttgg cgttgtccag caggtcgtcc tgcagatagg gattgcgctg caccacccag
       61 atgtcgccca gcacttcata gagcatgcgg gccgagcggc cggtgcgacg ctcctcgcgc
      121 agccggttca acacctccca ggcgcgctca cccagcagcc ggatcacgat ctcccggtcg
      181 gaaaacgagg tgtagttgta gggaatctcg cgcaggcggg gcgctgcgtc ttcttcggtc
      241 tggccggcga tctgggtcag cgggagcggt gcgttcatgg gcgcaaggcg cgcaatgcct
      301 ggggaagtgg gcggctggac gcctaaacga tgatggtacc gcagcgcagc aaatacgccg
      361 tagcgcggcc tgcggaacct ctgggcacgc agggtcgggc gggtaatccc cgttgtgacg
      421 ggatgtgcgt catgacagaa acgctcggca cgagccatgc ttctgtcacg agcgccgcct
      481 agactcgccg ccggccgcga cagtggccgc aaaaccttca cggagccgac atgaaactga
      541 aattcgcctc gctcgccggt gccgcctttc tcgcgctggc ctcagtccct gcccaagcgg
      601 tcaccgagat cacctggtgg cattccatgg agcaggccct gggcgagcgc gtcaacgaga
      661 tcgcgcgcca gttcaacgcc agccagtccg aatacaaggt cgtgcccgtc ttcaagggca
      721 actacgccga atccatggcc gccggcatcg ccgcctatcg cggcggcaat gcgcccgaca
      781 tcatccaggt cttcgaggtc ggtaccgcga ccatgatgag cgccaagggc gtgatcaagc
      841 cggtcgagga gctgatgcgc gaaaacggcg agaagttcga tcgcaacgct tacatccccg
      901 ccgtagccgg ctactacacc tcagccaagg gcgagatgct gtccttcccc ttcaacagct
      961 cgaccacggt gatgtattac aacaaggacg ccttcgccaa ggccgggctg gacgcgaaca
     1021 agccgcccgt cacctggcaa gagctggtgg ccgacgccgc caagctcaag gcctcgggcg
     1081 ccaagtgcgc ctacaccacg gcctggcagg cctggatcca gctggagagc ttctcgacgc
     1141 tgcacaacgt cgagttcgcg accaagaata acggcttcgg cggcggcgat gcgcgcctcg
     1201 tgttcaacag cccgctgcat gtgcgccaca tcgaggacat gggcaactgg gccaagcagg
     1261 gctatttcac ctatgccggc cgcaaggacg aaggcaatgc cagcttcttc agcggcgact
     1321 gcgcgatcct gaccgcctcg tccggcgcct acggcaacat cgccaagaac gccaaattca
     1381 agttcggcgt ggcccccctg ccctattaca gcgacgtgtc cggcgcgccg cagaaccccg
     1441 tcatcggcgg agccagcctg tgggtcatgg gcggcaagag caaggccgag tacaagggcg
     1501 tggccaagtt cctgagcttt ctgtcgcagc ccgagctgca ggccaagtgg caccaggaga
     1561 ccggctacct gcccatcacc accgccgcct atgagctgac caagaagtcc ggcttctacg
     1621 agcagaagcc cggcaccgac acggcggtca aggccatgat cgtcaagacc accgccaagt
     1681 cgcgcggcat ccgcctgggc aacatggcgc agatccgtga ggtgatcgac caggagctgg
     1741 aaaacgtctg gaacggcaag aagacggcca aggaggcgct ggacgaagcc gtgcggcgcg
     1801 gcaatgaatt gctcgagaag ttcgagaagg ccaacaaatc ctgacgagcc ctacagccgg
     1861 cccgccgctt gaggcgagcc ggccttaagc gatggaaaag cgcgcacgtt tcagctccgc
     1921 ctggctgccc tatgccctgg tcgcgccgca gatcgcggtg acgctggtgt tcttcttttg
     1981 gccggcggcg caggcgctgt ggcagtcgac cctgctgcaa gatgccttcg gggtgcagac
     2041 cgaattcgtc ggcctggaca acttcgtcgc gctctggaac gacccgcact acctggatgc
     2101 cttcaagacc acggccatgt tctcgctgct ggtggccgtc tcgggcatgg ccgtggcgct
     2161 gctgctggcc gtcttcgccg accgggtgct gcgtggcgcg cgctggtacc gcaccctgct
     2221 gatctggccc tatgccgtgt ccagcgtcgt ggccggcgtg ctgtggatgt tcctcttcag
     2281 cccctcgctg ggtctgctga cgcatgcatt ggcctggttc ggcatgactt ggaaccacaa
     2341 cgtcgacagc cgccaggcca tggcgctgat cgtgatcgcc gccacgtgga agcagatcag
     2401 ctacaacttc ctcttcttcc ttgcggggct gcagtcgatc ccgaagtcgc tgatcgaggc
     2461 cgcggcgatc gacggcgccg ggccggcacg gcgctttcgc gacatcgtct tcccgctgct
     2521 gtcgccgacc agcttcttcc tgctggtcat caacatcgtc tatgccttct tcgacacctt
     2581 cgcgatcatc gatatcacga cccatggcgg ccctggcacc gacaccgtga tcctggtcta
     2641 caaggtctac atggacggct tcaagggcca ggacttcggc ggctcggcgg cgcagtcggt
     2701 gatcctgatg ctgctcgtca tcacgctgac ggtgctgcag ttccgcttcg tcgaaaagaa
     2761 ggtccaatac tgaaatggtc gagcgccgcc cctggctcac gctgctgtcc cacctggtgc
     2821 tgatcgccgg tgtgctggtc gtcgtgttcc cgatctacgt cacgttcgtc gcgagcacgc
     2881 agaacgccga ccaggtgcgc caggtgccgc tgtcgctgct gcccggcagc gaggcgctgc
     2941 gcaactaccg cacggtgctg ttcggcgatg cagacaccac cggcgtctcg ccgatgggcc
     3001 acatgctgtg ggtcacgctg gtgatggcgc tgagcatcgc cgtcggcaag atcgcgatct
     3061 cgctgttgtc agctttcgcc atcgtctact tccgctttcc gggccgctcg ctgttcttct
     3121 ggatgatttt cgtcacgctg atgctgccgg tggaagtgcg tatctcgccg acctacaaga
     3181 tcgtctccga tctcggcctg ctcaacagtt atgccggcct gacgctgccc ctgatcgcct
     3241 cggccaccgc gaccttcctg ttccgccaat tcttccttac cgtgccggat gagctggcgg
     3301 aggccgcgcg cgtcgacggc gccggccccc tgcgctttat gcgcgacgtg ctgctgccgt
     3361 tgtcgctgac gaatatcgca gcgctcttcg tgatccagtt catctacggc tggaaccaat
     3421 acctctggcc gctgctggtg acgaacaacg aggacatgta tcccatcgtg ctgggcctga
     3481 agcgcagcat aggcgccggc ggcgacgccg cgatcgaatg gcagctcgtg atggccacgg
     3541 cgatgctcgc gatgctgccg ccggtgctgg tggtgatgct gatgcagaaa tggttcgtca
     3601 agggcctggt ggacgccgaa aagtaaatcg cataacggag cacgacatcc cgcatggcta
     3661 gcatctcctt gcgctcggtg gtcaagcgct acggccacgg ccccaaggcc ccccaggtga
     3721 tccacggcgt gaatgccgag atcgccgacg gcgaattcat cgtcatcgtc ggaccttcgg
     3781 gctgcggcaa gtccacgctg ctgcgcatgg tcgcggggct ggaggaaatc agcgacggcg
     3841 agatcgccat cggcccgcgc gtcgtcaacg agctggagcc ggccgagcgc gacatcgcga
     3901 tggtgttcca gaactacgcg ctctacccgc acatgagcgt gtacgacaac atggcctacg
     3961 ggctcaagat cgccaaggta ccggccgagg agatcaaggt ccgcgtcgac aaggccgcga
     4021 agatcctcga gctctcgcag ctgctcgaac gcaagccgcg ccagctctcc ggcggccagc
     4081 gacagcgcgt cgcgatggga cgcgccatcg tgcgccagcc gcaggtcttc ctgttcgacg
     4141 agccgctgtc caacctggac gccaagctgc gcgcgcagac gcggctggag atccagaaac
     4201 tgcatgccga gctgggtgtg acctcgctgt tcgtgacgca cgaccaggtc gaggccatga
     4261 cgctggcgca acgcatgatc gtgatgaacg ccggccgcat ggagcagatc ggcacgcctg
     4321 aagaggtcta tcagcgcccg gccaccacct tcgtcgccgg cttcatcggc tcgccgccga
     4381 tgaatctgct gctcgggcag gcccaggact cgcaattcgc catcgcaggc gcccggctcg
     4441 agctgccggc cgccgcaccg cgcgctggcg agctgatcct gggcgtgcgg cccgagcacc
     4501 tgcgcctggc cgagcagggc tggccggtga aggtcgagat gatggagatg ctgggcgccg
     4561 agcgcctggt ctacggccgg ctcggcggct ctccggacca cgggttgctg acgctgcgca
     4621 tggacgcgac cgaggcggcg cccggcgtcg gccagaccgt gcacctggca gcctcgcccg
     4681 agcgactgca ctggttcgac gcccggacgc aggaaagggt ggagcgatga gacatccagt
     4741 gtcgagttgt ctttcacggg ccgccgcgcc gggccgtccc aagcaaggcc cgaccctctt
     4801 ggagggtcga tcgccgtacc cggcgatcgg ggtgtggtca tgagccccgt gagcttcgac
     4861 gcctggcctt atcccctgtg gatcgcccac cgcggcgcgg gcaagctcgc gcccgagaac
     4921 acgctggccg ccttccgcct gggcgccgca catggctacc gcatgttcga gtgcgacgcc
     4981 aagctcagcg ccgacggcca gcccttcctg ctgcacgact cgacgttgag ccgtacgacc
     5041 aatgcccgcg gccctgccag cgccctgagc tgggacgagc tttctcggct cgacgcaggc
     5101 ggctggcaca gccgtgccta caccggcgag ccccttccct cgctgcaggc catcgcccgc
     5161 tatgtaaagc gcaacggttt cgcgctcaat atcgagatca agcccagccc cggcagcgag
     5221 cgccatacag gacgcgtggt cgccgaggcg gcggccgagc tgtggcgggg cgaggccttg
     5281 ccgccgctcc tgacctcctt caagccagag gccttgcaag gcgcgagcga agcggccccc
     5341 cacctgccgc gcggcctgct gctcgacgcg ctgcacggcg actgggtggg ccaggcgcag
     5401 accctgggct gcgtcgccat cgtcagccac tacggcctga tcgatgcagc gatggtggcg
     5461 cgcgtgcgag ccgcgcgcat gaagctcttg agctacacgg tcaacgaccc ggccgaggcc
     5521 tacaggctac acgggctggg ggtcgccggg ctgatcaccg atgccgtcga ccgcttcacg
     5581 ccgggtcccg gcgtgcagga ctgaggcata cggcccacca tgctataatt tcgggctcag
     5641 gcgctttagc tcagtcggtt agagcgacgg aatcataatc cgcaggtccg gggttcgagt
     5701 ccctgaagcg ccaccaaatc ccagaaggct tgtaggtcaa cgacttacaa gcctttttcc
     5761 ttgccaggga gtgccaactg gggggtgtac cgcaaacgct gcggcacgta ctttccgcgc
     5821 gtcattcccg ctgcaccagc ctcaatggct ctgcagcgga agaatgcggc tgccgtcatc
     5881 gctccacgcg atcgaagtag ctgcaggaga taccgagggc ttgatcgctg cgctgcagcg
     5941 cgctctggga tcgacttagc agagaattag cgctgaagcg cgcaccaata caccatgttg
     6001 agacacgtcg acgccgcact gctgggcgca tcgcactctt ccccgctggc tcgcagtctg
     6061 ccgcgacctc tcgccgagtt ggccttcttc ggcctcaagc aggcgcgcgc atgcctcttc
     6121 gccgccgtca tttttctcag cgtgttcatc gtgccccgcg gcggcctgtg gcatatcccg
     6181 cgctac
//
LOCUS       DASUCY010000200         2513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1000309, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000200 DASUCY010000000
VERSION     DASUCY010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000309"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VFV25_03900"
     CDS             <1..342
                     /locus_tag="VFV25_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007735013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase"
                     /protein_id="HEY1392489.1"
                     /translation="VVIFSFNLAIPISHLSSALASGTLGIALACVLMSFLMMITRAKA
                     VPQVIGFLAMENGLFFAATSATYGMPMVVELGIALDVLIGVLILGVFMFQIREQFDSL
                     DIRHLEKLKED"
     gene            344..1804
                     /locus_tag="VFV25_03905"
     CDS             344..1804
                     /locus_tag="VFV25_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HEY1392490.1"
                     /translation="MIALALLLGLPLLGGVVLALVGHRDNARDINVAFSAATFAAAAA
                     LTMQVIDHGPLLVWSREFYIDALNVFLVALTAFVGLTTSIFSRPYMRVERDHGKMTPP
                     RLRLYHSMYQLFNFTMLLALTTNNLGIVWVAMEAATLTTVLLVSVYRTAASLEAAWKY
                     FILCGVGIAQALFGTVLLYMAAEKVIGAEGGALLWTNLDAVKGQLDANIITLAFAFLF
                     IGYGTKVGLVPVHNWLPDAHAEGPTPVSAVLSGLLLNVALYAVLRCKVLTDGALQSPL
                     TGRLMMGFGLVSVVAAVFFLIRQKDVKRMFAYSSIEHMGMMTFAFGMGGPIANFAGLL
                     HMTVHSLVKSGIFFAVGHATQKAGTQVMDDIRGLVRVSPTVGWGLMLGTLAILGMPPF
                     GVFASEFLIVTTAMREHPWATPFLLIALGLAFASVFSRVQPMVFGETTLKPLAHPPAL
                     VPVFVHLALGLMLGLYMPPYLGAWYRQAAAMIGGGG"
     gene            1815..>2513
                     /locus_tag="VFV25_03910"
     CDS             1815..>2513
                     /locus_tag="VFV25_03910"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEY1392491.1"
                     /translation="MLVPLPTPVPIRHGVVDRDGWLAVAEDVLGGGGRLVSLWGSRRP
                     GGAVACAAYAFSSEGDLAWIELPLSDGEGYPDLARFFPAAARMQRAVHDLQGLSAEGA
                     ADTRPWLDHGLWQGQAEEAPYPFVRVEGEGVHEIAVGPVHAGIIEPGHFRFSVVGEKV
                     LRLEERLGYVHKGIEQRFTELAPLDAHRLAGRVSGDSTVAYAWAYCMALEGLTGCEVP
                     ERGLWLRALMLERER"
BASE COUNT          344 a          841 c          863 g          465 t
ORIGIN      
        1 gtggtgatct tctcgttcaa cctcgccatc cccatctcac acctgtcgtc ggcgctggcc
       61 agcggcaccc tgggcatcgc cctggcctgc gtgctgatgt ccttcctgat gatgatcacg
      121 cgcgccaagg cggtgccgca ggtgatcggc ttcctcgcga tggagaacgg cctgttcttc
      181 gccgccacgt cggctaccta cggcatgccg atggtggtgg agctcggcat cgcgctcgac
      241 gtgctgatcg gcgttctcat cctcggcgtg ttcatgttcc agatccgcga gcagttcgac
      301 agcctggaca tccgccatct cgagaagctg aaggaagact gaagtgatcg cgctggcgct
      361 gctgctcggc cttccgctcc tgggcggcgt ggtgctcgcc ctcgtcggcc accgcgacaa
      421 cgcgcgcgac atcaacgtgg ccttcagcgc cgccaccttc gccgcggccg cggcgctgac
      481 gatgcaggtg atcgaccacg gcccgctgct ggtgtggtcg cgcgagttct acatcgacgc
      541 gctgaacgtc ttcctcgtcg cgctgacggc cttcgtgggc ctcacgacct cgatcttctc
      601 gcgcccctac atgcgcgtcg agcgggacca cggcaagatg acgccgccgc ggctgcgcct
      661 gtaccacagc atgtaccagc tattcaactt cacgatgctg ctggcgctca ccaccaacaa
      721 cctcggcatc gtctgggtcg cgatggaggc ggccacgctg accaccgtgc tgctggtcag
      781 cgtctaccgc acggcggcca gcctggaagc ggcgtggaaa tacttcatcc tctgcggggt
      841 gggcatcgca caggcactgt tcggcaccgt gctgctgtac atggcggccg agaaggtgat
      901 cggcgcagag ggcggcgcac tgctgtggac caacctcgat gcggtcaagg gccagctcga
      961 cgccaacatc atcacgctgg ccttcgcctt cctgttcatc ggctacggca cgaaggtcgg
     1021 cctggtgccg gtgcacaact ggctgcccga tgcgcatgcc gaaggtccca cgccggtctc
     1081 ggccgtgctg tcgggcctgc tgctgaacgt ggcgctgtat gcggtgctgc gctgcaaggt
     1141 gctgaccgac ggcgccctgc aaagcccgct gaccggccgg ctgatgatgg gcttcggcct
     1201 cgtttccgtc gtcgcggcgg tgttcttcct catccggcag aaggacgtaa agcgcatgtt
     1261 cgcgtactcg tcgatcgagc acatgggcat gatgaccttc gccttcggca tgggcgggcc
     1321 gatcgccaac ttcgccggcc tgctgcacat gacggtgcac tcgctggtca agtccggcat
     1381 cttcttcgcg gtcggccacg cgacgcaaaa ggccggcact caggtgatgg acgacatccg
     1441 cgggctggtc cgcgtgagcc cgaccgtcgg ctggggcctt atgctgggca cgctggccat
     1501 cctcggcatg ccgcccttcg gcgtcttcgc gagcgagttc ctgatcgtca cgaccgcaat
     1561 gcgcgagcat ccgtgggcga cgcccttcct gctgatcgcc ctgggcctgg cgttcgcgtc
     1621 ggtgttcagc cgcgtgcagc cgatggtgtt cggcgaaaca acgctgaagc cgctggcgca
     1681 tccgcccgcg ctggttccgg tcttcgtgca cctggcgctc gggctgatgc tcgggctgta
     1741 catgccgccc tacctcgggg cctggtaccg ccaggcggcg gcgatgatcg gcggcggagg
     1801 ctgacgccat gtcgatgctg gtcccattgc cgacgcccgt gccgatccgc cacggcgtgg
     1861 tggatcgcga cggctggctg gcggtcgccg aagacgtgct cggcggcgga gggcggctgg
     1921 tctcgctttg gggcagccgg cgacccggcg gcgcggtggc ctgcgcggcc tatgcgttct
     1981 cgtccgaggg cgatctggca tggatcgagc tgccgctgtc cgacggcgaa ggctatcccg
     2041 acctggcacg cttcttcccc gccgcggccc gcatgcagcg tgcagtgcac gacctgcaag
     2101 gcctgagcgc agagggcgcg gccgacacgc gtccctggct cgatcacggc ctgtggcagg
     2161 gccaggccga agaggcgcct tatcccttcg tgcgggtcga gggcgaaggg gtgcacgaga
     2221 tcgcggtggg gccggtgcac gcgggcatca tcgaacccgg gcatttccgc ttctcggtgg
     2281 tcggtgagaa ggtcctgcgc ctggaggagc ggctgggcta tgtgcacaag ggcatcgagc
     2341 agcgcttcac cgagttggcc ccgctggatg cgcaccgcct cgccggtcgc gtgtcgggcg
     2401 actcgacggt ggcctatgcc tgggcctact gcatggcgct ggaaggcctc accggctgtg
     2461 aggtgcctga gcgcgggctg tggttgcggg cattgatgct ggagcgggaa cgg
//
LOCUS       DASUCY010000201         3071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_421804, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000201 DASUCY010000000
VERSION     DASUCY010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421804"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /gene="sbcB"
                     /locus_tag="VFV25_03915"
     CDS             complement(<1..958)
                     /gene="sbcB"
                     /locus_tag="VFV25_03915"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HEY1392492.1"
                     /translation="MTDFSFFWHDYETFGRVPRRDRPAQFAGIRTDAELNEIDAPVMH
                     YCRPAPDYLPDPESCLLTGILPQTCLERGLPEHEFAAAIEAELAAPGTLGVGYNSIRF
                     DDEVTRFLFWRNLIDPYAREWQNGCGRWDLLDVVRCTYALRPEGIRWPKQPDGRASFK
                     LEHLTGANGLAHEAAHDAASDVRATIALARLIKSKQPRLWEFCLRLSRKDAVLAEIGL
                     GEPRPFLHVSGMFPAERGCLGIVWPLGQHPSNKNELLVWDLAQDPGELFTLDAAAIRE
                     RLFSKAEDLPEGIARLPIKGLHINKSPVVIGNLKTLRPEMAQR"
     gene            1184..>3071
                     /gene="acnA"
                     /locus_tag="VFV25_03920"
     CDS             1184..>3071
                     /gene="acnA"
                     /locus_tag="VFV25_03920"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEY1392493.1"
                     /translation="MPADTRTQHAFANTLKSFKTASGKEGKFYSLPELAKQHPGVARL
                     PVSIRIVLESVLRNCDGNKVTAEHVQQLANWGAKAERVDEIPFVVARVVLQDFTGVPL
                     LADLAAMRNVADQMGKDPKTIEPLVPVDLVVDHSVMIDHYGNKKALDLNMKLEFQRNQ
                     ERYEFMKWGMQAFDTFGVVPPGFGIVHQVNLEYLARGVHKKDGVYYPDTLVGTDSHTT
                     MINGIGVVGWGVGGIEAEAGMLGQPVYFLTPDVVGFELSGRLREGVTATDLVLTVTEQ
                     LRREKVVGKFVEFFGEGTESLSVPDRATIANMAPEYGATMGFFPVDGKTIDYFKGTGR
                     TKPEIEAFEAYFKAQKMFGIPKAGDLDYTKVVKLDLGTVAPSLAGPKRPQDRIEIGNV
                     KSQFSSLFSKPAADNGFNQPAEKLGESFKTASGLEIRNGDVLIAAITSCTNTSNPGVL
                     LAAGLLAKKAVEAGLKVQPHIKTSLAPGSRIVTEYLEKAGLLPYLEKLGFDVAAYGCT
                     TCIGNAGDLTAEINEVITQNDLVCAAVLSGNRNFEARIHPNLKANFLASPPLVVAYAI
                     AGNVTRDLMTEPVGKGKGGRDVYLGDIWPSSDEVYKLMKHAMDPKAFRKNYEKVDREP
                     GKLWE"
BASE COUNT          566 a         1031 c         1008 g          466 t
ORIGIN      
        1 agcgctgcgc catctcgggc cgcaaggtct tgaggttgcc gatcacgacg ggcgacttgt
       61 tgatgtgcag gcccttgatc ggcagccgcg cgatcccctc gggcaagtcc tcggccttgc
      121 tgaacaggcg ctcgcggatc gccgcggcat ccagcgtgaa cagctcgccg ggatcctggg
      181 ccaggtccca taccagcagc tcgttcttgt tgctcggatg ctggcccagc ggccagacaa
      241 tccccaagca gccccgctcg gccgggaaca tgcccgagac atgcaggaaa ggccgcggct
      301 cgcccaggcc gatctcggcc agcaccgcgt ctttcctgct caggcgcagg cagaactccc
      361 acagccgcgg ctgcttggac ttgatgagcc gggccagcgc gatcgtcgcg cgcacgtcgg
      421 aggcggcgtc gtgcgccgcc tcgtgggcca ggccattggc acccgtcaga tgctcgagct
      481 tgaacgaggc gcgtccatcc ggctgcttgg gccagcggat gccttcgggt cgcagcgcat
      541 aggtgcagcg caccacgtcg agcagatccc agcgcccaca gccgttctgc cattcgcgcg
      601 cataggggtc gatcaggttg cgccagaaca ggaagcgcgt cacttcgtcg tcgaagcgga
      661 tgctgttgta gcccacgccc agggtgccgg gcgcggccag ctcggcttcg atcgcggcgg
      721 cgaactcgtg ctcgggcagg ccgcgctcca ggcaggtctg cggcaagatg ccggtgagca
      781 ggcaggactc ggggtcgggc aggtagtcgg gcgccggccg gcagtagtgc atgaccggcg
      841 cgtcgatctc gttcagctcg gcgtcggtgc ggatgccggc gaactgcgcc ggccggtcgc
      901 gccgcggcac gcggccgaag gtctcgtagt cgtgccagaa gaagctgaag tcggtcatgg
      961 ctgggctgga ggagcctgat tgctacaaaa agagggagca tcctccgcaa aatatgccgg
     1021 gcggcggatg atttttccat cacccacaag atcggcgatt ggcgccaagc atacacagcg
     1081 atgcactgcg gcaggccgca ggcgggcaca gggtttcatg cgtggccagc gtgccccgcc
     1141 gcataatcgt ttgaacgagt cccataaacc cgaggtccta aaaatgccag cagacacccg
     1201 cacacagcac gctttcgcca atacgctcaa gagcttcaag acggcctccg gcaaggaggg
     1261 caagttctac tcgctgcccg agctggccaa gcagcatccg ggcgtcgccc gcctgccggt
     1321 gtcgatacgc atcgtgctgg agtcggtgct gcgcaactgc gacggcaaca aggtgacggc
     1381 cgagcatgtg cagcagctcg ccaactgggg cgccaaggcc gagcgcgtcg acgagatccc
     1441 ctttgtggtc gcgcgcgtcg tgctgcagga cttcaccggc gtgccgctgc tggccgacct
     1501 ggccgcgatg cgcaacgtgg ccgaccagat gggcaaggac cccaagacca tcgagccgct
     1561 ggtgccggtg gacctcgtcg tcgaccactc ggtgatgatc gaccactacg gcaacaagaa
     1621 ggcgctggac ctcaacatga agctcgaatt ccagcgcaac caggagcgct acgagttcat
     1681 gaagtggggc atgcaggcct tcgacacctt cggcgtcgtg ccgcccggct tcggcatcgt
     1741 gcaccaggtc aacctcgaat acctggcgcg cggcgtgcac aagaaggacg gtgtctacta
     1801 ccccgacacc ctggtcggca ccgacagcca cacgacgatg atcaacggca tcggcgtggt
     1861 gggctggggc gtgggcggca tcgaggccga ggccggcatg ctgggccagc cggtctactt
     1921 cctcacgccc gacgtggtgg gcttcgagct cagcggccgg ctgcgcgagg gcgtgacggc
     1981 caccgacctg gtgctcaccg tcaccgagca gctgcgccgc gagaaggtgg tgggcaagtt
     2041 cgtcgagttc ttcggcgagg gcaccgagtc gctctccgtg cccgaccgcg cgaccatcgc
     2101 caacatggcg cccgaatacg gcgcgaccat gggcttcttc ccggtggacg gcaagaccat
     2161 cgattacttc aagggcaccg gccgcaccaa gcccgagatc gaagccttcg aggcctattt
     2221 caaagcgcag aagatgttcg gcatccccaa ggccggcgac ctcgactaca ccaaggtggt
     2281 caagctcgac ctcggcacgg tcgcgccctc gctggccggc cccaagcgtc cgcaggaccg
     2341 catcgagatc ggcaacgtca agagccagtt cagctcgctg ttcagcaagc ccgcggccga
     2401 caacggcttc aaccagccgg ccgagaagct cggcgagtcc ttcaagaccg ccagcggcct
     2461 cgagatccgc aacggcgacg tgctgatcgc cgccatcacc tcctgcacca acacctccaa
     2521 tcccggcgtg ctgctggctg cgggcctgct ggccaagaag gcggtcgagg ccgggctcaa
     2581 ggtgcagccg catatcaaga cctcgctggc gcccggctcg cgcatcgtca ccgagtacct
     2641 ggagaaagcg ggcctgctgc cctatctcga gaagctgggc ttcgacgtgg cggcctacgg
     2701 ctgcacgacc tgcatcggca atgccggcga cctgacggcc gagatcaatg aggtcatcac
     2761 ccagaacgat ctcgtctgcg ccgccgtgct ctcgggcaac cgcaacttcg aggcgcgcat
     2821 ccaccccaac ctgaaggcga acttcctcgc ctcgccgccg ctggtcgtgg cctatgcgat
     2881 cgccggcaat gtcacgcgcg acctgatgac cgaaccggtc ggcaagggca agggcgggcg
     2941 cgacgtctac ctcggcgaca tctggccgag cagcgacgag gtctacaagc tgatgaagca
     3001 cgcgatggac ccgaaagcct tccgcaagaa ttacgagaag gtcgacaggg agccgggcaa
     3061 gctgtgggag c
//
LOCUS       DASUCY010000202         2892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1160943, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000202 DASUCY010000000
VERSION     DASUCY010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160943"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1135)
                     /locus_tag="VFV25_03925"
     CDS             complement(<1..1135)
                     /locus_tag="VFV25_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEY1392494.1"
                     /translation="MLLKRFLAALALAGATLAFAAAPATPLPRGLTQITSAEGITEYR
                     LANGLQVLLVPDDSKPTTTVNVTYHVGSRMENYGETGMAHLLEHLMFKGSPKHPTVWA
                     EFTKRGLRANGSTWFDRTNYFASFSANEDNLRWYLGWQADAMVNSYIARKDLDSEMTV
                     VRNEMEMGENSPDRVLLDKTLAAMYQWHNYGKSTIGARADVENVDIPRLQAFYRQYYQ
                     PDNATLIVAGKFDVAKTLAWIAQDFGVIPKPKRVLPKLYTIDAVQDGERSVTLRRTGG
                     VPLLYAGYHVPPAAHPDYAAIEALGLIMADTPAGRLHKHLVEKQLAAGVFAFSEGLHD
                     PGFVILGAQLAPGQDLDKARDEMLATLEDAAKEPITEDELKRAQ"
     gene            complement(1269..1808)
                     /gene="ppa"
                     /locus_tag="VFV25_03930"
     CDS             complement(1269..1808)
                     /gene="ppa"
                     /locus_tag="VFV25_03930"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEY1392495.1"
                     /translation="MSLHNVTPGVKAPEEFNVIIEIPMNADPIKYEVDKETGAIFVDR
                     FMTTAMHYPCNYGYVPQTLSDDGDPVDVLVITPFALTPGVVVTCRPIGVLKMEDEAGG
                     DAKLLAVPTTKILPIYDHWKKPEDINPMRLKAIQHFFEHYKDLEAGKWVKVQGWEGPE
                     AAKQEVREGMANYAKAKSA"
     gene            2066..>2892
                     /locus_tag="VFV25_03935"
     CDS             2066..>2892
                     /locus_tag="VFV25_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HEY1392496.1"
                     /translation="MSPSWALIFALLCGLAAVLYGFVQRSWILKQDAGNARMQEIASA
                     VQQGAAAYLARQYKTIAMVGVVLAVLIAIFIGLPTAAAFVVGAILSGACGFIGMNVSV
                     RANVRTAQAATRGIGEALDVAFKGGAITGMLVVGLGLLGVSVVFWVLTRNGAGADLAT
                     TIKPLLGLAFGASLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVIADN
                     VGDNVGDCAGMAADLFESYAVMLVVGLALLGVAGYYIVLRQLTSVGAEGALHALVGLA
                     "
BASE COUNT          432 a          902 c         1007 g          551 t
ORIGIN      
        1 cctgggcgcg cttgagctcg tcctcggtga tcggctcctt ggcggcgtct tcgagggtcg
       61 cgagcatttc gtcgcgcgcc ttgtccaggt cttgccctgg cgcgagctgg gcgcccagga
      121 tcacgaagcc cgggtcgtgc agcccttcac tgaaggcgaa cacgccggct gcgagctgct
      181 tctcgaccag gtgcttgtgc agccggccgg ccggggtgtc ggccatgatg aggccgagcg
      241 cctcgatggc cgcgtagtcg gggtgggcgg ccggcggcac gtggtagccc gcgtacagca
      301 gcggcacgcc gccggtgcgg cgcagcgtca cactgcgctc gccgtcctgc accgcatcga
      361 tggtgtagag cttgggcagc acgcgcttgg gcttggggat gacgccgaag tcctgggcga
      421 tccacgccag cgtcttggcc acgtcgaact tgccggccac gatcagcgtg gcgttgtcgg
      481 gctggtagta ctggcgatag aaggcctgca gccgcgggat gtcgacgttc tcgacgtcgg
      541 cgcgcgcacc tatggtcgac ttgccgtagt tgtgccactg gtacatggcg gccagcgtct
      601 tgtcgagcag cacgcggtcg gggctgttct cacccatctc catctcgttg cgcaccacgg
      661 tcatctcgct gtcgaggtcc ttgcgcgcta tgtagctgtt gaccatcgca tcggcctgcc
      721 agccgaggta ccagcgcagg ttgtcctcgt tggccgagaa gctcgcgaaa tagttggtgc
      781 gatcgaacca ggtggagccg ttggcgcgca ggccgcgctt ggtgaactcg gcccacacgg
      841 tcggatgctt gggcgagccc ttgaacatca ggtgctcgag gaggtgcgcc atgccggtct
      901 cgccgtagtt ctccatgcgt gagccgacgt ggtaggtcac gttgaccgtg gtcgtgggct
      961 tggagtcgtc gggcaccagc agcacttgca ggccgttggc gagccggtat tcggtgatgc
     1021 cctcggcgga agtgatctgc gtgaggccgc gtggcaaggg agtggcgggt gccgcggcaa
     1081 aggcgagcgt ggcgccggca agtgccaggg ccgccaggaa acgcttcaac agcatctgag
     1141 tgctccttga gggaaggcgc agtgtagcca cgcccgttgc aggcatacgc cacatttccg
     1201 cattcgaggg acaaaaagga aaacgggtcc gaccggaccc gttttcgagg caagaggcgc
     1261 gcgttggttc aggcgctctt cgctttcgcg tagttggcca tgccctcgcg cacttcctgc
     1321 ttggcggctt ccggcccttc ccagccctgc accttgaccc acttgccggc ctccaggtcc
     1381 ttgtagtgct cgaagaaatg ctggatcgcc ttcaggcgca tcgggttgat gtcctcgggc
     1441 ttcttccagt ggtcgtagat cggcaggatc ttggtggtgg gcaccgcgag cagcttggcg
     1501 tcgccgccgg cctcgtcttc catcttcagc acgccgatcg gccggcaggt gacgaccacg
     1561 ccgggcgtca gcgcgaaagg cgtgatcacc agcacgtcca ccgggtcgcc gtcgtccgac
     1621 agggtctgcg gcacatagcc gtagttgcag gggtagtgca tggccgtcgt catgaagcgg
     1681 tcgacgaaga tggccccggt ttccttgtcg acctcgtact tgatcgggtc ggcgttcatc
     1741 gggatctcga tgatgacgtt gaactcttcg ggcgccttga cgccgggggt cacgttgtgc
     1801 aggctcatgc gggggctctt ttaaatgatg atgaatgcca gggataaccc gaagtttact
     1861 gcccaggctt gcacagtcct tgcagacggt tgcggcagcg caagccacag gaaaacacca
     1921 tggacgcgtc attgccttga taaagggcat cacttagcat gagccgcgcg acccggccgg
     1981 ccgctgacgc ggtgtcaaca agaagaaagc ccgggcgtac cttcatcatc atcacactcc
     2041 cggactacgg aagaggagac gctacatgtc cccatcttgg gcgcttattt ttgcgctgct
     2101 gtgcggtttg gcggctgtgc tgtacggttt cgtgcagcgc agctggatcc tgaaacagga
     2161 cgcgggcaat gcccgcatgc aggagatcgc ctcggcggtc cagcaaggcg cggcggccta
     2221 tctggctcgc cagtacaaga ccatcgccat ggtcggcgtg gtgctggccg tgctgatcgc
     2281 gatcttcatc ggcctgccga cggcggcggc cttcgtggtc ggcgcgatcc tgtcgggcgc
     2341 ctgcggcttc ataggcatga acgtctcggt gcgcgcgaac gtgcgcaccg cgcaggccgc
     2401 cacgcgcggc atcggcgagg cactcgacgt ggccttcaag ggcggcgcga tcaccggcat
     2461 gctggtggtg ggcctgggcc tcctgggcgt gagcgtggtg ttctgggtgc tgacgcgcaa
     2521 cggcgccggc gccgatctcg ccaccacgat caagccgctg ctgggcctgg ccttcggcgc
     2581 ctcgctgatc tccatcttcg cgcgcctggg cggcggcatc ttcaccaagg gcgccgacgt
     2641 gggcgccgac ctggtgggca aggtcgaggc cgggatcccg gaggacgatc cgcgcaaccc
     2701 cgcagtgatc gccgacaacg tgggcgacaa cgtgggcgac tgcgccggca tggcggccga
     2761 cctgttcgag tcctacgcgg tcatgctggt cgtggggctg gcgctgctcg gtgtcgccgg
     2821 ctactacatc gtgctgcgcc agctcacctc ggtaggcgcc gagggcgcgc tgcacgcgct
     2881 ggtggggctc gc
//
LOCUS       DASUCY010000203         2620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1562394, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000203 DASUCY010000000
VERSION     DASUCY010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1562394"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2620)
                     /locus_tag="VFV25_03940"
     CDS             complement(<1..>2620)
                     /locus_tag="VFV25_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEY1392497.1"
                     /translation="LPVFFPSNVQDILDMGLHAFAMSRFSGVWTGMKTIQEVVESSAS
                     VSVDPDRVNIVLPEDFIMPPGGLHIRWPDAPLEQEARLMDYKWYAALAYVRANKLNHN
                     VIAGPNDRFGIIASGKAFNDTRQALADLGLDDDTCRAIGLRLHKVNVVWPLEATITRD
                     FALGLQEILVVEEKRQVIEYQLKEELYNWRADVRPHVLGKFDAPDNGSGNEDEGGEWS
                     MPNPSQNWLLRAKADLTPAIIAKAIAKRLKKMGVSADIAARMDARIAVIEARERSLAE
                     VKTDTGERAPWFCSGCPHNTSTRVPEGSRAVAGIGCHYMTVWMDRSTSTFTQMGGEGV
                     PWVGQQPFTSEKHIFANLGDGTYFHSGLLAIRQSIAAGVNITYKVLYNDAVAMTGGQQ
                     VGERPEGHSVMQIMQSLVSEGVAKLVIVTDEPAKYEGAALSPGVTVHHRDELDAIQRQ
                     FRELPGTTIIIYDQTCATEKRRRRKRGKLADPAKRVVINELVCEGCGDCSVQSNCLSV
                     EPVETEFGRKRRINQNSCNKDYSCVKGFCPSFVTVEGGQLKKPKKGGKAGDLSALPQL
                     PEPVLPAAEQAWGIVVGGVGGTGVITIGQLLGMAAHLEGKGVVTQDAGGLAQKGGATW
                     SHIQIANRPEAIHTTKVDTAKADLVIGCDPIVAANKYTLAVMQPGRTYVALNSHGTPT
                     AAFVSNPNWQFPGGNCEAAIEAAVGKPMVGAFDAEQVAVQLLGDSIYTNPLMLGYAWQ
                     QGRIPLGRAALMRAIELNGVQADNNKAAFEWGRRCAHDLASVRSLFKAAQVIEFVKKP
                     SVAETIAKRADFLTGYQDAAYAADYRAFVEKVRAAEAKLDGKGTRLSEAVARYLFKLM
                     AYKDEYEVARLHTDRA"
BASE COUNT          364 a          876 c          896 g          484 t
ORIGIN      
        1 aaggcgcggt cggtgtgcag gcgcgcgact tcgtactcgt ccttgtaggc catcagcttg
       61 aagaggtagc gtgccactgc ctcgctcagg cgagtgccct tgccgtcgag cttcgcctcg
      121 gctgcgcgca ccttctcgac gaaggcccgg tagtcggccg cataggcggc gtcctggtag
      181 ccggtgagga agtctgcacg cttggcgatc gtctcggcca cggagggctt cttgacgaac
      241 tcgatcacct gcgccgcctt gaacagcgag cgcaccgaag cgaggtcatg tgcgcagcgg
      301 cggccccatt cgaaggcggc cttgttgttg tcggcctgca cgccgttgag ctcgatggcg
      361 cgcatcagcg cggcgcggcc gagcgggatg cggccctgct gccaggcata gcccagcatc
      421 agcgggttgg tgtagatcga gtcgcccagc agctgcacag cgacctgctc ggcatcgaag
      481 gcgccgacca tgggcttgcc cacggccgcc tcgatcgccg cctcgcaatt gccgccgggg
      541 aactgccagt tggggttgct gacgaaggcc gccgtcggcg tgccatgcga gttgagcgcg
      601 acataggtgc gcccgggctg catcacggcc agcgtgtact tgttggccgc cacgatgggg
      661 tcgcagccga tcacgagatc ggccttggcg gtgtcgacct tggtggtgtg gatggcctcg
      721 ggccggttgg cgatctggat gtggctccag gtcgcgccgc ccttttgcgc caggccgccg
      781 gcatcctgcg tgacgacgcc cttgccttcc aggtgggccg ccatgcccag cagctggccg
      841 atggtgatga cgccggtgcc gcccacgccg ccgaccacga tgccccaagc ctgctccgcc
      901 gccggcagca ccggttcggg cagctggggc agcgccgaca agtcgcccgc cttgccgccc
      961 ttcttgggct tcttcagctg gccgccctcg acggtgacga agctcgggca gaagcccttc
     1021 acgcaggaat agtccttgtt gcagctgttc tggttgatgc ggcgcttgcg gccgaactcg
     1081 gtctccaccg gctcgaccga gaggcagttg ctctgcaccg agcagtcgcc gcagccttcg
     1141 cagaccagct cgttgatgac gacgcgcttg gccggatcgg ccagcttgcc gcgcttgcgg
     1201 cggcggcgct tctcggtggc gcaggtctgg tcgtagatga tgatcgtggt gccggggagc
     1261 tcgcggaact ggcgctggat cgcatccagt tcgtcgcggt gatgcaccgt cacgccgggc
     1321 gaaagcgccg cgccttcgta cttggccggc tcgtcggtca cgatcacgag cttggccacg
     1381 ccctcggaga ccaggctttg catgatctgc atcacgctgt gtccctcggg ccgctcgccg
     1441 acctgctggc cgccggtcat cgccactgcg tcgttgtaga gcaccttgta agtgatgttc
     1501 acgcccgcgg cgatgctctg gcggatcgcc agcaggccgc tgtggaagta ggtgccgtcg
     1561 cccaggttgg cgaagatgtg cttctcgctc gtgaagggct gctggccgac ccagggcacg
     1621 ccttcgccgc ccatctgcgt gaaggtggag gtgctgcggt ccatccacac cgtcatgtag
     1681 tggcagccga tgccggccac cgcgcgcgag ccctcgggca cgcgggtgct ggtgttgtgc
     1741 gggcagccgc tgcagaacca gggcgcacgc tcgccggtgt cggtcttgac ctcggccagg
     1801 gaccgctcgc gcgcctcgat gacggcgatg cgcgcatcca tgcgcgcggc gatgtcggcg
     1861 ctcacgccca tcttcttcag ccgcttggcg atcgccttgg cgatgatggc cggcgtcagg
     1921 tcggccttgg cgcgcagcag ccagttctgg ctgggattgg gcatgctcca ctcgccgccc
     1981 tcatcctcgt ttccgctgcc gttgtccggc gcgtcgaact tgcccagcac atgggggcgc
     2041 acgtcggcgc gccagttgta gagctcttcc ttgagctggt attcgatgac ctggcgcttc
     2101 tcctcgacca ccaggatctc ctgcagcccg agcgcgaagt cgcgtgtgat ggtcgcctcc
     2161 agcggccaca ccacgttgac cttgtgcagg cgcaggccga tcgcgcggca ggtgtcgtcg
     2221 tccaggccca ggtcggccag cgcctggcgc gtatcgttga aggccttgcc gctggcgatg
     2281 attccgaagc gatcattcgg gccggcgatg acgttgtggt tgagcttgtt ggcgcgcaca
     2341 taggccagcg ccgcatacca cttgtagtcc atcagccgcg cttcctgctc cagcggcgca
     2401 tcgggccagc ggatgtgcag gccgcctggc ggcatgatga agtcctccgg cagcacgatg
     2461 ttcacccggt ccgggtcgac cgagaccgag gccgaggact cgaccacctc ctggatggtc
     2521 ttcatgccgg tccacacgcc cgagaagcgg ctcatcgcga aggcgtgcag gcccatatcg
     2581 aggatgtcct gcacgttgga cgggaagaac accggcaggc
//
LOCUS       DASUCY010000204         5765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_197043, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000204 DASUCY010000000
VERSION     DASUCY010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 5765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5765
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197043"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1908)
                     /gene="tssM"
                     /locus_tag="VFV25_03945"
     CDS             complement(<1..1908)
                     /gene="tssM"
                     /locus_tag="VFV25_03945"
                     /inference="COORDINATES: protein motif:HMM:TIGR03348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HEY1392498.1"
                     /translation="MTTPRTGGSPLPQFLLSLVGLLAAGTLVWFVGPLIALAGTTPLA
                     GEGQRWIVIAAMGSLAVMHTAWRAIQAARNNRRLMEGMASAARREEEPQAPPGGADAS
                     VQTPGEQEVALLGERFERAVGMLKRRRVGGRGPLLAALTGRPYVYELPWYIIIGAPGA
                     GKTTALANSGLEFPLASQLGKKVIRGVGGTRNCDWWFAADAVLIDTAGRYTTHDSDRA
                     TDRAAWLGFLSLLKQYRPARPINGVLLTISVSDLLNSNEGQRLAHARALRERIEELHA
                     QLGIRFPIYVMVTKTDLLAGFMEFFADFDKDERAQVWGVTFPYDTELSSDDPLARMAS
                     DFAALEKLLNQCLIDRLHAEHDRERRPAVYAFPQQWAVLRVMLLGFLQAVFAEANGGL
                     RPLMRGVYFTSATQEGTPIDRAIGGLARALGLSGRLVAPARPSGKTFFVTRLLREVVL
                     SEAGLAGTDLRWRRRRARLRWALAGATACVTAGAVALAWFAYQDSRARIAAVAERMAD
                     LGHDAAVARNSAPTDLVALLPSLQSLQALEAVQAATRASDEASQHRWSWLSMGLGRGE
                     MLAAAAHDAYERLLKEVFLPRVAARLEERLRAASPEHVERIYEDLKAYLMLFGGKNFD
                     KAALRAFLGADW"
     gene            complement(1905..3215)
                     /gene="tssL"
                     /locus_tag="VFV25_03950"
     CDS             complement(1905..3215)
                     /gene="tssL"
                     /locus_tag="VFV25_03950"
                     /inference="COORDINATES: protein motif:HMM:NF038228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, long
                     form"
                     /protein_id="HEY1392499.1"
                     /translation="MTSDDERTVVWGPDHTILMPTPGGQATVVLKRPEVAPTARPGPE
                     IELQRLVAGINPLLGAANVLLGLVEQLRSTGSHADPAGLRLQLLERVREFESVARANG
                     VPAHQISAARYVLCSFVDEVIAATPWGAGGVWAQRNLLQEFHQERSGADKSFKLLERL
                     CEEPATNHDLLELFYVCLSLGFEGRYRGTRNGHQQLDAIAARVLELVRPAREQQATRT
                     LSLRWQGVAAQAQRSLTGLPLWVLLALAGALVLGSLLMLNVRLDRMAEPVFKQIHALT
                     AELQVERAATAAARPRLAQALRADMAASSVEVQDEAQRSLLTLSADTLFEGGSARIEP
                     SRLELLGRIAQALRLQPGLVVVVGHSDDNPTASLQFPSNWHLSRERALAVAASLVQHG
                     LPADRLRAEGRADAEPRVPNATPAGRAKNRRIEIELLLPRPDPT"
     gene            complement(3287..4699)
                     /locus_tag="VFV25_03955"
     CDS             complement(3287..4699)
                     /locus_tag="VFV25_03955"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY1392500.1"
                     /translation="MPANIPERLGKYPVVGVLGKGAMGTVFLAQDPIIKRRVAIKAIR
                     RELIEDDEKLDTVPGRFRREAQAAGGLNHPGIVAIYEYGEDQQYAYIAMEYVEGNTLR
                     DYLQNGHSFDERDTVSIMAQLLEALEFAHRNNVWHRDIKPANIMIMSNGRIKLTDFGI
                     AHVEAVDRTRTNLLMGTPGYIAPEYYLGEHIDHRIDIFSAGVLFYELLAREAPFRGRS
                     EAIMHDVCYHDPPPPSEIDPEHRWPRYDEVVARAIEKAPPRRFQSAEEFRAAVLAEYA
                     RPVSDTLSEATIISLKLAQPAGADSTPAPKTPSYPGTSTPPPTGWTAPVLAGVERELA
                     RFVGPVARVLVRRAAQLHKDIDSLVGALLDSLDSSKDREAFARAVTGHAVALPSQADG
                     DSRPGLAQETPWPAPALTPADVEQATRLLTAYIGPIARVVAKRAAGMGLNRRDFFNEV
                     ARSVDNEGQRERFLQEAGNG"
     gene            complement(4739..5611)
                     /locus_tag="VFV25_03960"
     CDS             complement(4739..5611)
                     /locus_tag="VFV25_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HEY1392501.1"
                     /translation="MKLLFKFNLVFLLVFAVAIVAAGRISWTLLERNAREEIAGSARL
                     LMDAALAARNYTTGQVGPLLDTQMKYSFLPQSVPAYAATEVFNDLRKKHAEYSFKEAV
                     LNPTNPRDRAVEWESDIVNQFRGNKDLGELLGDRETPTGGSFYIARPIRISDPSCLRC
                     HSTVDAAPKTMIERYGPANGFDWVFNDVVGARIISVPTSVPLERAQHAFKVFMGSIAA
                     VFVAIGVVLNLMLWLLVIRPIGALSRFTDRVSLGELEIPDFKRASRDEIGVLALSIGR
                     MRTSLVKAMKMLET"
BASE COUNT          906 a         1954 c         1980 g          925 t
ORIGIN      
        1 ccagtcagcg ccgaggaagg cccgcaaggc cgccttgtcg aagttcttgc cgccgaacag
       61 catcaggtag gccttcaggt cctcgtagat gcgctcgaca tgctcggggc tggccgcacg
      121 cagcctttct tccagccgcg cggcgacgcg cggcaggaag acctccttga gcagccgctc
      181 ataggcgtcg tgcgcggcgg cggcaagcat ctcgccccgc cccaggccca tggacagcca
      241 ggaccatcgg tgctgggatg cctcatcgct cgcgcgcgtg gcggcctgca cagcctccag
      301 ggcctgcagc gactgcaagc tgggcagcag ggcgaccagg tcggtcggcg cagagttgcg
      361 cgccactgca gcgtcgtgcc cgaggtccgc catgcgttcg gccaccgcgg cgatgcgggc
      421 ccggctgtcc tggtaggcaa accacgccag tgccacggca ccggcggtga cgcaagcggt
      481 cgccccggcc agcgcccagc gcagtcgcgc gcggcggcgg cgccagcgca gatcggtccc
      541 ggccagtccg gcctcggaca gaacgacctc gcgcagcagc cgggtcacga agaaggtctt
      601 gccgctcggc cgcgccggag ccacgaggcg gccggataag ccgagtgcgc gcgccagccc
      661 cccgatcgca cgatcgatcg gcgtgccctc ctgcgtggca ctcgtgaagt aaaccccgcg
      721 catcagcggg cgcagcccgc cattggcttc ggcaaagacg gcttgcaaga agccgagcag
      781 catgacgcgc agcaccgccc attgctgcgg aaaggcataa acagcggggc gccgctcgcg
      841 atcgtgctcg gcatgcagcc tgtcgatcag acactggttg aggagctttt cgagcgctgc
      901 gaaatcgctc gccatgcgcg ccagcggatc gtcggagctc agctcggtgt cgtaggggaa
      961 ggtcacaccc cagacttgtg cgcgctcgtc cttgtcgaag tcggcaaaga actccatgaa
     1021 gccagcgagc agatcggtct tggtgaccat cacgtagatc gggaaacgga tgccgagctg
     1081 cgcgtgcagc tcctcgatgc gctcgcgcag tgccctagcg tgagccaggc gctggccctc
     1141 gttgctgttc agcaggtcgc tgacgctgat ggtgagcagc acgccattga tcggccgggc
     1201 cggccggtac tgcttcaaca aactgagaaa accgagccag gcggcgcggt cggtcgcgcg
     1261 atcgctgtcg tgcgtggtgt agcggccggc agtgtcgatc agcacggcgt cggcggcgaa
     1321 ccaccagtcg caattgcggg tcccgccgac gccgcgtatc accttcttgc cgagttggct
     1381 ggcgagcgga aactcgagtc cggaattggc cagcgcagtc gtcttgccgg ctcccggcgc
     1441 gccgatgatg atgtaccagg gcagctcgta gacatagggc cggccagtga gcgcagccag
     1501 cagcggccct cggcccccca cgcgacgccg cttgagcatg cccactgcgc gctcgaagcg
     1561 ctctccgagc aaagctacct cctgctcgcc gggtgtctgc accgaggcat cggcgccccc
     1621 gggcggcgct tgcggttctt cctcgcgccg ggcggcggat gccatgccct ccatcagccg
     1681 ccggttgttg cgcgcggcct ggatcgcgcg ccacgccgta tgcatcacag cgaggctgcc
     1741 catggccgca atgacgatcc agcgctgtcc ttcgccagct agaggcgtcg ttccggcgag
     1801 cgcgatcagc gggccgacga accagaccag agtgcccgct gcaagaaggc cgaccaggct
     1861 cagcagaaat tgcggcagag ggctgccgcc cgtacgcggc gtcgtcatgt cgggtccggc
     1921 cgcggcagca gcagttcgat ctcgatacgg cggttcttcg cccgcccggc cggggtcgcg
     1981 ttgggaacgc gcggctcggc atcggcgcgc ccctcggcgc gcaggcgatc ggccggcagg
     2041 ccgtgttgca ccaggctggc cgcgaccgcc agcgcgcgct cgcgcgacag atgccagttc
     2101 gagggaaact gaagcgaagc ggtcgggttg tcgtcgctgt gcccgaccac gacgacgagc
     2161 ccaggctgca gcctgagtgc ctgagcgatg cgcccgagaa gctcgagccg gctcggctcg
     2221 atgcgcgcgc tgccgccctc gaagagcgtg tcggccgaca gcgtcagcag cgagcgctgc
     2281 gcctcgtcct gcacctcgac gctgctggcg gccatgtcgg cccgcagcgc ctgcgccagc
     2341 cgaggcctgg ccgccgccgt cgccgcgcgc tcgacctgca gctcggccgt caacgcgtgg
     2401 atctgcttga acacaggctc agccatacga tccagccgca cgttcagcat cagcaagctg
     2461 ccgagcacga gggccccggc cagcgcgagc agcacccaaa gcggcaggcc cgtgaggctg
     2521 cgttgggctt gcgcggccac gccctgccag cgcagtgaca gcgtgcgcgt ggcctgctgc
     2581 tcccgcgccg ggcgcaccag ctcgagcacg cgagcggcga tggcgtcgag ctgctgatgg
     2641 ccgtttctcg tgccgcgata gcggccttcg aagccgagcg agaggcaaac gtagaacagc
     2701 tccagcagat cgtgattggt cgccggctcc tcgcaaagtc gttcgagcag cttgaaggac
     2761 ttgtctgcgc ccgagcgctc ctggtggaac tcctgcagca ggttgcgctg cgcccagacg
     2821 ccgcccgccc cccagggggt ggcggcgatg acctcgtcga cgaagctgca cagcacatag
     2881 cgcgcggccg agatctggtg cgccggcacg ccattggcgc gggccaccga ttcgaattcc
     2941 cgcactcgct cgagcagctg cagccgcagg ccggcagggt cggcatgcga gccggtcgag
     3001 cgcagctgct ccaccagccc cagcagcaca ttggccgcgc ccagcagagg gttgatgccc
     3061 gccacgaggc gctgcagctc aatctccggg cccggccggg ctgtcggcgc tacctcaggc
     3121 cgcttgagaa ccacggtcgc ctgcccgcct ggggtgggca tcaggatcgt gtgatccggg
     3181 ccccagacga cggtgcgttc atcgtcgctc gtcatggtct gccgccgctg gctgattggg
     3241 tttggcgatg gctgggccgc agggcgtgcg tcggcctgct tcggcttcaa ccgttcccgg
     3301 cctcttgcag gaagcgctcg cgttgccctt cgttgtcgac gctgcgggcg acttcgttga
     3361 agaagtcgcg ccggttgagg cccatgccgg cggcccgctt ggcgaccacg cgcgcgatcg
     3421 ggccgatgta ggccgtcaac aacctggttg cctgctccac gtctgctggc gtcagagccg
     3481 gagcgggcca cggcgtttcc tgcgccaggc cggggcgcga gtcaccgtcg gcctgggacg
     3541 gcaaggcaac cgcatgcccg gtcaccgccc gggcgaaggc ctcgcggtcc ttgctgctgt
     3601 cgagcgaatc gagcaaggcg cccaccagcg aatcgatgtc cttgtggagc tgagctgcgc
     3661 gccgcaccag cactcgggcg accgggccga cgaatcgcgc gagttctctc tccacgccgg
     3721 ccagcaccgg cgccgtccag ccggtgggcg ggggcgtgct ggtgcccgga taagagggag
     3781 tcttgggtgc cggcgtggag tcggcaccgg ccggctgcgc gagcttcaag ctgatgatgg
     3841 tcgcctcgga cagggtatcg ctcaccgggc gcgcgtattc ggccagcaca gcggcgcgga
     3901 actcttcagc gctctggaaa cgccgcggcg gcgccttctc gatggcgcgg gcgacgacct
     3961 cgtcatagcg cggccagcga tgctccggat cgatctccga gggtggcggc ggatcgtgat
     4021 agcagacgtc gtgcatgatc gcctcggagc ggccgcggaa aggcgcctca cgggccagca
     4081 attcgtagaa cagcacccca gccgagaaga tgtcgatgcg gtgatcgatg tgctcgccga
     4141 ggtagtactc gggcgcgata tagcccggag ttcccatcag caaattggtg cgcgtgcggt
     4201 cgaccgcctc gacatgggcg atgccgaaat cggtgagctt gatccggccg ttgctcatga
     4261 tcatgatgtt ggccggcttg atgtcgcggt gccacacgtt gttgcggtgc gcgaactcca
     4321 gcgcctccag cagctgggcc atgatgctca cggtgtcgcg ctcgtcgaag ctgtggccgt
     4381 tctgcaggta gtcgcgcagc gtattgccct cgacatactc catcgcgatg taggcgtact
     4441 gctggtcttc gccgtattcg tagatggcga cgatgccggg atggttgagc ccgcccgccg
     4501 cctgggcctc gcggcgaaag cgcccgggca cggtgtccag cttctcgtcg tcctcgatca
     4561 gctccctgcg tatggccttg atcgccaccc gccgcttgat gatggggtcc tgggccagga
     4621 agacggtgcc cattgccccc ttgccgagca cgccaacaac cggatacttg cccagacgct
     4681 cagggatgtt ggccggcatg agtggactcg ggtttcgggg ggcgagatgg cgggccgctc
     4741 aggtttccag catcttcatc gccttgacca ggctggtccg catgcggcct atcgatagtg
     4801 caagcacgcc gatctcgtcg cgcgaagccc gcttgaagtc cgggatctcg agctcgccca
     4861 gactcacgcg atccgtgaag cgcgacagag cgccgatcgg gcggatgacg agcagccaca
     4921 gcatgaggtt gagcacgacg ccaatggcga caaacaccgc ggcgatcgaa cccatgaaga
     4981 ccttgaaggc atgctgggcc cgctcgagcg gcacgctggt cggtaccgag atgatgcgcg
     5041 ctcccaccac gtcgttgaag acccagtcga agccgttggc tggcccgtag cgctcgatca
     5101 tggtcttggg cgcagcgtcc accgtgctgt ggcagcgcag acaactcggg tcactgatgc
     5161 ggattggccg cgcgatatag aaggagcccc cggtgggcgt ctcgcggtcg cccagcagct
     5221 cgcccaggtc cttgttgccg cggaactggt tgacgatgtc gctttcccat tccaccgcgc
     5281 gatcgcgcgg attggtcggg ttgagcaccg cctccttgaa gctgtactcc gcgtgtttct
     5341 tgcgcaggtc gttgaacacc tccgtcgccg cataggccgg cacggattgc ggcaggaagc
     5401 tgtacttcat ctgcgtgtcc agcagcggcc cgacctggcc agtcgtgtag ttgcgcgctg
     5461 ccagcgccgc gtccatcagc aggcgtgcgc ttccggcaat ttcctcgcgc gcgttgcgct
     5521 ccagcaaggt ccacgatatg cgcccggcgg ccacgatcgc cacggcgaag accagcagga
     5581 acacgaggtt gaacttgaag agcaacttca cggcggcctc cgtcatcggt tggaggaagg
     5641 ggcgagcgcg ctggcggcgt tctctgccgc ggcttggggc cgccgcgccg catgctcctt
     5701 gggcaggtat ttctcgggga acaagatcgg atcccattcc gggtgggccg acaggttggc
     5761 gatca
//
LOCUS       DASUCY010000205         2477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_566677, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000205 DASUCY010000000
VERSION     DASUCY010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566677"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2131
                     /locus_tag="VFV25_03965"
     CDS             <1..2131
                     /locus_tag="VFV25_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007741017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEY1392502.1"
                     /translation="YLRMGGWGTTTVISSGKDVYIHYAAPEFAFALANDARSKAAVLY
                     CEPGGYYEHDAHFTKPVVACVVGRWKSKLTRAVGHAGAMAGGNDDAQAKERWFMEKVG
                     VDALFTPDNPVVSAKGAVVTNIAHIPAALTAVMRANATMPDFPAEGNLALKPWFGSDE
                     GLALPEELRQPVVEALSPYNEQVALLNRQIGALVPRQTMKDASGASQMDPKTQVSSLH
                     GTSMLHAATLSLESNVALALVHDAGGENDRKLIAPAVAAYVNLHGQPELAAAQAGRET
                     GNAPNAVLAAAAAIVGPKRQEAARTALKFMIERFHLAGLGMEFGASLSEDFDIARVDA
                     SGQPGLTTNTPDPRAQALLAGVQARGARSVFLRWLQSLPGHPTEAAVLAAVSATLAWG
                     PLSRKRISRATAESFPWWLQLFGTLIGASADASRHEAGRFCGIAKEQLLGSLSLGEIA
                     FAALLGRTPGEDDLFAFQTLIGLLLTNGPGAISAQGAKGAVSADGPEQPERVQLNKAL
                     IGFLTHTGYAHGGNGYEGIAYLNEQFKDSGLADPSAKNHGIDLAALAARAVEQYAQYK
                     AQKKTLGSLDIAKLPGVNHPVFKDKPVNFDPREVFIAKMFEERGETNVFHAFYRALVQ
                     QLYEAGVSRNVYCVNIDAVIAALLLKMLWKPLQAGEIGERDLENAAFTIFLYPRMLGC
                     AAEIDDHLNRGRNMDTRTPASQCRYVA"
     gene            2205..>2477
                     /locus_tag="VFV25_03970"
     CDS             2205..>2477
                     /locus_tag="VFV25_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392503.1"
                     /translation="MDESRIAFAQACEAAAEQIRSRIKCTRVTFWVVAGEPGRFALKR
                     IATDLTKTSSLEIEEVRIDSTVFKERFCVSNDTLAAPSFANVVASFL"
BASE COUNT          418 a          854 c          838 g          367 t
ORIGIN      
        1 gtacctgcgc atgggcggat ggggcaccac caccgtgatc tccagcggca aggacgtcta
       61 catccactat gctgcgcccg agttcgcctt tgcgctcgcc aacgacgcgc gcagcaaggc
      121 cgcagtgctg tactgcgagc cgggcggcta ctacgagcat gacgcgcact tcaccaagcc
      181 agtggtggcc tgcgtggtgg gtcgctggaa gagcaagctc acgcgcgccg tcggccatgc
      241 cggcgcgatg gccggcggca acgacgacgc gcaggccaag gaacgctggt tcatggagaa
      301 agtcggcgtc gatgcgctgt tcacgcccga caaccccgtg gtctcggcca agggtgcggt
      361 ggtgaccaac attgcgcaca tcccggccgc gctgacggcc gtgatgcgcg ccaacgccac
      421 catgccggac ttcccggccg agggcaacct ggcgctcaag ccctggttcg gctccgacga
      481 agggctggcc ctgcccgaag aattgcgcca gcccgtggtg gaggcgctgt cgccctacaa
      541 cgagcaggtg gcgctgctta accgccagat cggcgccctc gtgccgcgtc agaccatgaa
      601 ggacgcctcg ggcgcctcgc agatggaccc gaagacacag gtcagcagcc tgcacggcac
      661 cagcatgctg cacgccgcga cgctgtcgct ggaatcgaac gtcgcgctgg cgctggtgca
      721 cgacgcgggc ggcgagaatg accgcaagct gatcgcgccg gctgtcgcgg cctacgtcaa
      781 cctccatggc cagcccgaac tggcggcggc ccaggccggc cgcgagaccg gcaatgcacc
      841 caatgccgtg ctggcggcgg cggcggccat cgtcgggccg aagcgccagg aggcggcacg
      901 caccgcgctc aagttcatga tcgagcgctt ccacctggcc ggtctcggca tggagttcgg
      961 cgcctcgctg agcgaggact tcgacatcgc ccgcgtcgac gccagcggcc agccgggcct
     1021 gaccaccaac acgcccgacc cgcgtgcgca ggcgctgctg gccggcgtgc aggcgcgcgg
     1081 cgcgcgctcg gtgttcctgc gctggctgca gagcctgccg ggccatccga ccgaggccgc
     1141 cgtgctggca gcggtctcgg ccacgctggc ctggggcccg ctgtcgcgca agcgcatctc
     1201 gcgcgccacg gccgagagct tcccctggtg gctgcagctg ttcggcacgc tgatcggcgc
     1261 ttcggcggat gcgtccaggc acgaggccgg gcgtttttgc ggcatcgcga aggagcaact
     1321 gctgggcagc ctgagcctgg gcgagatcgc gttcgcagcg ctgctcggcc gcacgccggg
     1381 cgaggacgac ctgttcgcgt tccagacgct gatcggcctg ctgctcacca acggccccgg
     1441 ggcgatctcg gcacagggcg ccaagggcgc ggtgtcggcc gacggccccg agcagcccga
     1501 gcgcgtgcag ctcaacaagg cgctgatcgg ctttctcacg cacaccggct acgcgcacgg
     1561 cggcaacggc tacgaaggca tcgcctacct caacgagcag ttcaaggaca gcggactcgc
     1621 ggacccgagc gcgaagaacc acggcatcga tctcgcggcg ctcgcggcca gggcggtcga
     1681 gcagtacgcg cagtacaagg cccagaagaa gacgctgggc agcttggaca tcgccaagct
     1741 gcccggcgtg aaccacccgg tgttcaagga caagccggtc aacttcgatc cgcgcgaggt
     1801 gttcattgcc aagatgttcg aggaacgcgg cgagaccaac gtgttccacg cgttctaccg
     1861 cgcgctggtg cagcagctct acgaggccgg cgtctcgcgc aacgtgtact gcgtcaacat
     1921 cgacgcggtg atcgccgcct tgctgctgaa gatgctgtgg aagccgttgc aggccggcga
     1981 gatcggtgag cgcgacctgg agaacgccgc gttcaccatc ttcctgtacc cgcgcatgct
     2041 cggctgcgcc gccgaaatcg acgatcacct gaaccgtggc cgcaacatgg acacgcgcac
     2101 gccggcctcg cagtgccggt atgtcgcctg agacccgcct tcaatgatcc ggacccccgc
     2161 ctaccgtgac tgacccagag gagctcgtcg agcttggtcg cggcttggac gagagccgca
     2221 tcgccttcgc ccaggcctgt gaagcggctg cggagcagat ccggtcgcga atcaagtgca
     2281 cccgcgtcac gttctgggtg gtcgccggcg agccgggcag gttcgccctg aagcgcatcg
     2341 cgacggattt gacgaagact tcttctttgg agatcgagga agtccgcatt gacagtacag
     2401 tgttcaagga gcgcttctgc gtcagcaacg ataccttggc ggcgccgagc ttcgccaacg
     2461 tcgtcgccag cttcctg
//
LOCUS       DASUCY010000206         2930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_116689, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000206 DASUCY010000000
VERSION     DASUCY010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116689"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..960
                     /locus_tag="VFV25_03975"
     CDS             118..960
                     /locus_tag="VFV25_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HEY1392504.1"
                     /translation="MSYATITDLPAPLSDGLQAGALAPYLGPGMLSLCSSVPVPATPL
                     DLAAFLTAKVSVPGKIRNRLTAAAQFIENFKHRKTLVTAMDEAYAATPAPSALHRWVA
                     ALPAPLIVDGWYDNTLRSALSTRTDWAEVQGLSQSEHFGTWTGWYTAAGDASPDTIEA
                     PTVLYKPWGGRAPASNYLVSDTDYVEVLTEIDIQTPIPSLVQQRRSRLGFVFLGCRFN
                     DQLPRAFARQIIKRSKGPHYAVLPDEPTRMEARFLDEQGITRVALPLAEAAGRLIASA
                     LVAS"
     gene            993..1298
                     /locus_tag="VFV25_03980"
     CDS             993..1298
                     /locus_tag="VFV25_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY1392505.1"
                     /translation="MATLTILPSGKTVEAETGASLLKAILDAGEKILTKCGGEAKCGA
                     CHIFVTAGRKGVSKMTPAENAKLDTIVGVGSKSRLACQATLLGTEPVTVELLGALSG"
     gene            1439..>2930
                     /locus_tag="VFV25_03985"
     CDS             1439..>2930
                     /locus_tag="VFV25_03985"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY1392506.1"
                     /translation="MLDVAIAGGGLCGLALAHSLQARGCDWRLFEARERLGGRVLTAK
                     ARDGTPVDLGPTWFWPQTQPAITRLVADLGLHSFAQHDDGRVLHLSDPNRAPQAVALT
                     EQLQPAADASVPAAPGAVHGGAWRVAGGMAAVIEALARPLPATRLRLGYRVEAVIDHG
                     DFVELRMRYGDVSLALNARRVVLALPPRVVEASVHFVPELPAALRAALQRTPTWMATA
                     AKAGFAYARPFWREAKHTGNAWVSHAQAMLSEVFDACGPEAGVSRYPGAALAGFSALG
                     AAQRPSFRRGRALLLESQVVQLFGPEAADPELLWQDWAEEETTCSARDIAEEGLPSDG
                     HPAYGDPLLAQAQWDGRLYFGGSETVRQGGGYLEGALGAAARLRHQLLSGSAASLRPQ
                     QAANAELVIDTGNEMHLQAFAAWVQAERGRVLARYRERLHQALSRQDDAQLTQRAVLG
                     ALESLYEESLQQINGLPPAAVERVLQPFMGLSDELLAEAVIFNRTSC"
BASE COUNT          464 a         1051 c          984 g          431 t
ORIGIN      
        1 cgctgccgct gcacacccac ctcgaggccg accacgtgaa gttcatcgtc aagacgatga
       61 aggacagctc ggtcaacgtc ggcgccgggg cggcgatcta ctgaaaggct gcatcccatg
      121 agctacgcca ccatcaccga cctacccgcc cccctcagcg acggcctgca agccggcgcc
      181 ctcgcgccct acctcggccc gggcatgctg agcctatgca gcagcgtgcc ggtaccggcc
      241 acgccgctgg acctggccgc cttcctcacg gccaaggtgt cggtacccgg caagatccgc
      301 aatcgcctca ccgccgctgc gcagttcatc gagaacttca agcaccgcaa gacgctcgtc
      361 accgcgatgg acgaggccta tgcggcaacg cccgcgccat cggcactgca ccgctgggtg
      421 gcggcgctac ccgcgccgct gatcgtcgac ggctggtacg acaacacgct gcgtagtgca
      481 ttgagcacac gcaccgactg ggccgaggtg cagggcctgt cgcagtccga gcactttggc
      541 acctggactg ggtggtacac ggcggcgggc gacgcgagtc ccgacacgat cgaggcgccc
      601 acagtgctct acaagccctg gggcggccgc gcgccggcga gcaactacct cgtgtccgac
      661 accgactacg tggaggtgct caccgagatc gacatccaga cgccgatccc gtcgctcgtg
      721 caacagcgcc gttcgcggct tggcttcgtc ttcctcggct gccgcttcaa cgaccagctg
      781 ccgcgcgcct tcgcgcgcca gatcatcaag cgctcaaaag ggccgcacta cgcggtgctg
      841 cccgacgaac ccacgcgcat ggaagcgcgc ttcctcgacg agcagggaat cactcgagtg
      901 gcgctgccgc tcgccgaagc cgccgggcgg ctcattgcca gcgcactggt cgcaagctga
      961 tttcacgtcc cacatcagga gatgcataca tcatggccac gctcaccatc ctccccagcg
     1021 gaaaaaccgt cgaggccgag accggcgctt cgctgctcaa ggccatcctc gacgcaggcg
     1081 agaagatcct caccaagtgc ggcggcgagg ccaagtgcgg cgcctgccac atcttcgtca
     1141 cggcgggccg caagggcgtt tcgaagatga cgccggccga gaatgccaag ctcgacacca
     1201 tcgtcggcgt gggcagcaag tcgcgcctgg catgccaggc tacgctgctg ggcaccgagc
     1261 cggtgacggt agagctgctg ggtgcgctgt cgggttgaaa tggcccaccc cctacggcct
     1321 tcggccgccc cctcaagggg gcgacgccgg cggatcggca aagccggatc cgcggtgttc
     1381 gcttggttag gccggttcat gcgtcgcggc tcgcgcgaaa cgcggtggag caactgagat
     1441 gctggacgtc gccatcgccg gcggcgggct gtgcggcctg gccttggcgc acagcttgca
     1501 ggcgcgaggg tgcgactggc gcctgttcga ggcacgagag cgcctcggcg ggcgcgtgct
     1561 gacggccaag gctcgcgacg gcacgccggt agacctgggg cccacttggt tctggccgca
     1621 gacccagccg gccatcaccc gcctcgtcgc ggacctgggg ctgcacagtt tcgcgcagca
     1681 cgacgacggc cgcgttctgc acctgtccga ccccaaccgc gcgccgcagg cggtggcact
     1741 gaccgaacag ctgcagcccg cggccgacgc gtcggtgcct gcagcgccag gcgcggtgca
     1801 cggcggagca tggcgcgtgg ccggcggcat ggccgcggtg atcgaggcgc tggcgcgacc
     1861 gctgcccgcc acgcgcctgc gcctgggcta ccgtgtcgag gccgtcattg accatggcga
     1921 cttcgtcgag ctgcgcatgc gctacggcga cgtctcgctc gccctcaacg cgcggcgcgt
     1981 agtccttgcg ttgccaccgc gcgtggttga ggcgagcgtc cacttcgtac ccgaactgcc
     2041 tgccgcattg cgtgccgccc tgcaacgcac gcccacctgg atggcgactg ccgccaaggc
     2101 cggcttcgcc tatgcgcgac ccttctggcg cgaggccaag cacaccggca acgcctgggt
     2161 cagccatgcg caggccatgc tctcggaagt gttcgacgcc tgcgggccgg aggccggtgt
     2221 gtcgcgctac cccggcgccg cactggccgg cttttcagcg ctcggcgcgg cgcagcgccc
     2281 cagcttccgg cgcggccgcg ccttgctgct cgaaagccag gtcgtgcagc tcttcgggcc
     2341 ggaagcggct gacccagagc tcctctggca agactgggcc gaggaagaga ccacctgcag
     2401 cgcgagagac atcgccgagg agggcctgcc gtcggacggc cacccggcct acggcgaccc
     2461 gctgcttgcg caagcgcagt gggacggacg gctctacttc ggcggctccg agaccgtgcg
     2521 ccaaggcggc ggctatctgg aaggcgcgct cggcgcggcg gccaggctgc gccatcagct
     2581 gctctctggc agtgcggcca gcctgcgccc gcagcaggcg gccaatgccg agctcgtaat
     2641 cgatacaggc aacgaaatgc atctgcaagc cttcgccgcc tgggtgcagg ccgagcgggg
     2701 ccgtgtcctg gcgcgctacc gcgagcggct gcaccaagcc ttgtcgaggc aggacgacgc
     2761 gcagctcacg cagcgtgcgg tgctgggtgc gctggaatcg ctctatgaag agtccctgca
     2821 gcagatcaat gggttgccgc cggcggccgt ggagcgcgtg ttgcagccct tcatgggctt
     2881 gtccgacgaa ctccttgcgg aggcggtgat cttcaaccgc acatcctgcg
//
LOCUS       DASUCY010000207         3544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_502313, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000207 DASUCY010000000
VERSION     DASUCY010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502313"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="VFV25_03990"
     CDS             <1..574
                     /locus_tag="VFV25_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY1392507.1"
                     /translation="LFERTDLVEIIRHAVSTYGVPHRAIELEITETSLMKDLQGVIPS
                     LHRLNEIGVEISIDDFGTGYSSLAYLTSLPISELKIDRSFVRDLGVTLQSSAVVTAII
                     ALSRSLGLRVVAEGVESMRQMEVLHRLGCSVMQGFLFSRALPPEELEQWLQQTVLPRK
                     APWIGKAGEGDADLLQQVTGTRAARSNPGA"
     gene            810..2030
                     /locus_tag="VFV25_03995"
     CDS             810..2030
                     /locus_tag="VFV25_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY1392508.1"
                     /translation="MQWSRAARAAACAGVLLAAVGLVQAQAQTQAAKPVRIGYICPST
                     GGSADFGNSARLGAELAVKEINEVGGYLGRPIELVERDDKANPDEGRRAAEELVLKEK
                     VDFTVGYCNTGVALKSLDVFQDHKHLLVVPVATGSAITAKYPAASSYIFRMSARDTLQ
                     AGFLVDEVVKRGFTKVAIFADKTGYGEGGVTDVERFLAEKKLKPAYVGRFELGVKSLV
                     DEMREAKAAGADAIIGYTVGPEQAVLAQARTEAGFKGPLYGAWPMSFRTVSESAGAAS
                     EGAMMVQTIIQDISNERRSSFIARLKRFAGKQPVDSLMAAAQSYDAVQLMLRVMFQTK
                     GDTSGDALKRALENLQSSYAGVVTTHDKPFSPTDHDAFTRNMIWLGVWRHGEIHFFYP
                     DDAKHVGYVRRKEQ"
     gene            complement(2071..3159)
                     /locus_tag="VFV25_04000"
     CDS             complement(2071..3159)
                     /locus_tag="VFV25_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011295970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEY1392509.1"
                     /translation="MNATVLNESSPSFSLFGLPDEAPHERVLLASDAHTGLKAILAIH
                     STARGPAFGGCRFWSYEREAAALTDALRLAQGMSLKNALADLPFGGGKAVILKPAHGD
                     FDREALFAAFGRAVQSLDGAYITAEDVGTTTADMRIVQKQTPHVSGIPREGGAFGGDP
                     SPKTAWGVFVAIETGVRQHLKRASLEGVSVAIQGLGAVGMHLAAQLHAAGAKLTVADV
                     AAARVSEARERFGAAAVPVNEILHADVDVLAPCALGAALNAQTIPGIRARLVAGAANN
                     QLATPADGDALHARGVMYLPDYLVNAGGIISVAREYRGEGNEKAVMSEVTRIGERVSE
                     LLERVLSSGQTPARVADAWARSKLVRPA"
     gene            complement(3410..>3544)
                     /locus_tag="VFV25_04005"
     CDS             complement(3410..>3544)
                     /locus_tag="VFV25_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345705.1"
                     /note="catalyzes the formation of
                     3,6-dideoxy-D-glycero-D-glycero-4-hexulose; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HEY1392510.1"
                     /translation="HQVYACGAPIMVESARRDFAAQCGLPGEEFYSDAFTSEADKHGA
                     "
BASE COUNT          558 a         1160 c         1261 g          565 t
ORIGIN      
        1 gctgttcgag cgcaccgacc tggtcgagat cattcgtcac gcggtgagca cctacggcgt
       61 gccgcaccgt gccatcgagc tggaaatcac cgagaccagc ctgatgaagg atctgcaggg
      121 tgtgatccct tcgctgcata ggctcaacga gatcggcgtc gagatctcga tcgacgactt
      181 cggcaccggc tactcctcgc tcgcctatct cacgtcgctg ccgatcagcg aactcaagat
      241 cgaccgcagc ttcgtgcgcg acctgggcgt gacgctgcag agctcggcgg tggtgacggc
      301 catcatcgcg ctgagccgct cgctgggcct gcgtgtcgtg gccgagggcg tcgaaagcat
      361 gcgccagatg gaggtgctgc accgcctggg ctgctcggtg atgcagggct ttctgttcag
      421 ccgcgccttg ccgcccgaag agctggagca gtggctgcag cagaccgtgc tgccgcgcaa
      481 ggcgccgtgg atcggcaagg ccggcgaggg agacgccgat ctccttcagc aggtgaccgg
      541 cacgcgcgcg gcgcggagca atccgggagc ctgagccccg tggcgggggc cgttggcggc
      601 ccgccacttt gggggcggca ttgggcggca ttaattcacg atctgctccc ggggactacc
      661 ctaggcgatg cttctctcgc ggcaattccc tcgccgccct ctaagctaga ccccccgaat
      721 caccgggccg gctggcgaac tcgcgcccgg cctcggacaa ggttcggcag cggcctggcg
      781 ccgcctggca cacaacaaga ggagacatca tgcaatggag cagggctgcc cgggcggcag
      841 cttgcgctgg cgtcttgctg gccgccgtcg gtctcgtgca ggcgcaggcg cagacgcagg
      901 cagccaagcc tgtgcgcatc ggctacatct gtccttccac gggcggctct gccgacttcg
      961 gcaacagcgc gcgccttggg gccgagctgg ccgtcaagga gatcaacgaa gtcggcggct
     1021 atctcggccg gccgatcgaa ctggtcgaac gcgacgacaa ggccaatccc gacgaggggc
     1081 gcagggcggc cgaagagctg gtgctgaagg agaaggtcga cttcaccgtc ggctactgca
     1141 acaccggcgt ggcgctgaag tcgctcgacg tgttccagga ccacaagcat ctgctggtcg
     1201 tgcccgtcgc cacgggctcg gcgatcaccg ccaagtatcc ggccgcgagc agctacatct
     1261 tccgcatgtc ggcgcgcgat accttgcagg ccggcttcct ggtggacgag gtggtcaagc
     1321 gcggctttac caaggtcgcg atcttcgccg acaagaccgg ctatggcgaa ggcggcgtca
     1381 cggatgtcga gcgtttcctg gcggagaaga agctcaagcc ggcctatgtg ggccgcttcg
     1441 aactgggcgt gaagtccctg gtcgacgaga tgcgcgaggc caaggcggcg ggggccgacg
     1501 cgatcatcgg ctacaccgtg ggcccggagc aggcggtgct ggcacaggct cgcacggaag
     1561 ccggcttcaa ggggccgctc tatggggcct ggccgatgtc cttccgcacc gtcagcgaga
     1621 gcgccggcgc ggcctccgag ggcgcaatga tggtgcagac catcatccag gacatcagca
     1681 acgagcggcg cagcagcttc atcgcgcggc tcaagcgctt tgccggcaag cagcccgtcg
     1741 attcgctgat ggcggcggcg cagtcctacg acgcggtgca gctgatgctg cgcgtgatgt
     1801 tccagaccaa gggcgacact tccggcgatg ccttgaagcg ggcgctggaa aatctgcaga
     1861 gctcgtatgc aggcgtcgtc acgacgcacg acaagccgtt ttcacccacc gatcacgatg
     1921 ccttcacgcg caacatgatc tggctgggcg tctggcggca tggcgagatc catttcttct
     1981 atcccgacga cgccaagcat gtcggctacg tgcggcgcaa ggagcagtag ggcgcggccc
     2041 gcctgcgaag gccggccgct gccgatttgc ttaagcaggg cggactagct tggaccgtgc
     2101 ccaggcgtcg gccacgcgcg ccggtgtctg gcccgagctg agcacccgct ccagcagctc
     2161 gctgacgcgc tcgccgatgc gggtgacttc ggacatcacg gccttttcgt tgccttcgcc
     2221 gcggtattcg cgggccacgc tgatgatgcc gccggcattg accaggtagt cgggcaggta
     2281 catcacgccg cgcgcatgca gcgcgtcgcc gtcggccggc gtggcgagct ggttgttggc
     2341 ggcgccggcc accagtctgg cgcgaatgcc ggggatggtc tgtgcattga gcgccgcgcc
     2401 cagggcgcag ggcgccagca cgtccacgtc ggcatgcagg atctcattga cgggcaccgc
     2461 cgcggcgccg aagcgctcgc gcgcctcgct gacgcgcgcg gccgcgacgt cggcgacggt
     2521 gagcttggcg ccggccgcgt gcagctgcgc tgccaagtgc atgccgaccg cgcccaggcc
     2581 ctggatggcc acgctgacgc cctcgagcga ggcgcgcttg aggtgctggc gcacgccggt
     2641 ctcgatcgcg acgaacacgc cccaggccgt cttgggcgag gggtcgccgc cgaaggcgcc
     2701 gccttcgcgc gggatgccgc tgacgtgagg cgtctgcttc tgcacgatgc gcatgtcggc
     2761 cgtcgtcgtg cccacgtcct cggccgtgat gtaggcgccg tccagcgact gcacggcgcg
     2821 gccgaaggcg gcgaacaggg cctcgcggtc gaagtcgccg tgtgccggct tgaggatcac
     2881 cgccttgccg ccgccgaagg gcaggtcggc cagcgcgttc ttcaggctca tgccctgggc
     2941 caggcgcagc gcatcggtca gcgccgcggc ttcgcgctcg tagctccaga agcggcagcc
     3001 gccgaaggcc gggccgcgcg cggtgctgtg gatcgcgagg atggccttga ggccggtgtg
     3061 cgcgtcgctg gccagcagca cgcgctcatg gggggcttcg tcgggcaggc cgaaaaggga
     3121 aaaagagggg gaggactcgt tcaggacggt ggcgttcatc tcgttggcac tcgtgatgcg
     3181 tgggaaggga cgccctggcg ggcgcccgtg gccccgcggc ttgtggctgc ggggtggcag
     3241 agcttaacgc agtacgaatg cggcgctcac gccgactcgg catacgggta tgccgagtgc
     3301 gcatgggcct taaggcgagc gcctaggctc cgtgcttgtg tctccggcag gcgctgcgcg
     3361 ccttaacttc gctcgccgag cgaagtgagc ctacgacgca aacatggggc taggccccat
     3421 gtttgtcagc ctcgctggtg aaggcgtccg aatagaactc ctcgcccggc aagccgcatt
     3481 gcgcggcgaa atcgcggcgg gccgactcga ccatgatggg cgcgccgcag gcatagacct
     3541 ggtg
//
LOCUS       DASUCY010000208         2248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1080664, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000208 DASUCY010000000
VERSION     DASUCY010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080664"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..1853)
                     /locus_tag="VFV25_04010"
     CDS             complement(33..1853)
                     /locus_tag="VFV25_04010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY1392511.1"
                     /translation="MKAALRPGRRVLLWHALLVLGAIALLAINWWVHARMLGLHRLHA
                     HGNEVLADLAQLERATAPQPALLRCAVSKGDAGPPSSLPPSGGMPGAALDPRPVWQRL
                     RALTAERPEQQRSLDALLPLFEALNQQYLQPLAAACGATAPTSPEQALAISAAGLEQR
                     SRILAVVQDMRQAETSLRGEREAGLRGSDERSDGLFSLLALATLALAVTAGVALRRFM
                     ARLADADTRVQHEAAERDNAQQRQRDSQRRLQMVMDHIPDAVIAFDEQGSVQWINPAG
                     EAMFASRHDVVRSHPVWRLVPELEFWIHWPDTQPQDEAMPAGLPWTVRRELMLGLRED
                     GREFPIDISVVQTRAYGERIGVCVCRDLSELERMERMKHEFISMVSHELRTPLTSIRG
                     SLSMLADGGAGELSAPVRRLVTLAHSNTERLVALVNDILDFEKLRAGEMRMQLETLCL
                     DDAARAAIEACEGYAHEHGVGLSLDLHATRLSVEADALRLGQVLANLLSNAVKFSPEG
                     GQVRAAVGGEGGRAWVSVGDDGPGVPPELVDQLFLQPFVQADQLATRARGGTGLGLAI
                     SKALMDQMGGQIGFEAPQPGRGATFFVSLPLRPDQAALPS"
     gene            complement(1850..>2248)
                     /locus_tag="VFV25_04015"
     CDS             complement(1850..>2248)
                     /locus_tag="VFV25_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY1392512.1"
                     /translation="GAVGWLACPVMLVFEGFAPVVELLGYLTMTVLAVTGLLSWAAFG
                     AFMMLALGMGMVLSACALLLEELAFRTYPKPRHLVQLLAAIVVESCGYRQINAWWRIV
                     GLTQWVTQRKQRWGDMQRVGSGQPGQSPGA"
BASE COUNT          356 a          819 c          761 g          312 t
ORIGIN      
        1 agtaactcgg cgagtctagc ccagcgccca tcctatgaag gcagtgcggc ctgatccggc
       61 cgcagaggca gcgaaacgaa gaaggtcgcg ccgcggccgg gctgcggcgc ctcgaagccg
      121 atctggccgc ccatctggtc catcagcgcc ttgctgatcg caaggccgag gcccgtgccg
      181 ccgcgcgcgc gtgtcgcgag ctggtcggcc tgcacgaagg gctggagaaa gagctgatcg
      241 acgagctcgg gcggaacgcc cggcccatcg tcgccgacgg acacccaggc ccgcccgcct
      301 tcgccgccca ccgcggcgcg cacctggcca ccctcgggcg agaacttcac cgcattgctc
      361 agcagattgg cgaggacctg gcccagtcgc aaggcatcgg cttccacact caggcgcgtc
      421 gcatgcagat ccaggctcag cccgaccccg tgctcatgcg cgtagccctc gcaagcctcg
      481 atcgcggccc gggccgcatc gtcgaggcag agggtttcca gctgcatgcg catttcgccg
      541 gcgcgcagct tttcgaagtc gagtatgtcg ttcaccagcg caaccaggcg ctcggtgttg
      601 ctgtgcgcga gcgtgaccag gcgccgcacc ggcgcactca gctcgcccgc gccgccgtcc
      661 gccagcatcg atagcgagcc gcggatgctg gtcagcggcg tgcgcagctc gtggctgacc
      721 atggagatga actcgtgctt catgcgctcc atgcgctcga gctcgctgag gtcgcggcat
      781 acgcacacgc cgatgcgctc gccataggcg cgcgtctgca ccaccgagat gtcgatcggg
      841 aattcgcggc cgtcttcgcg caggcccagc atgagttccc ggcgcaccgt ccagggcagc
      901 ccggccggca tcgcttcgtc ctgcggctgg gtgtcgggcc agtggatcca gaactccagc
      961 tccggcacca ggcgccacac ggggtgcgag cgcacgacgt catgccggct cgcgaacatg
     1021 gcctcgccgg ccgggttgat ccactgcacc gaaccctgct cgtcgaaggc gatcaccgcg
     1081 tcgggaatgt ggtccatcac catctgcagc cggcgctgcg aatcgcgctg gcgctgctgg
     1141 gcgttgtcgc gctcggcggc ctcgtgctgc acgcgggtgt cggcatcggc cagccgggcc
     1201 atgaagcgcc gcagcgccac gcccgcggtg accgccaagg ccagcgtggc cagcgccagc
     1261 aatgaaaaca gcccgtcgga gcgctcatcg ctgccgcgca gccccgcctc gcgttcgccg
     1321 cgcaggctgg tttcggcctg gcgcatgtcc tgtaccaccg ccaggatgcg actgcgctgc
     1381 tcgaggcctg cagccgaaat cgcgagcgcc tgctcggggc tcgtgggcgc tgtggccccg
     1441 caggccgccg cgagcggctg gagatattgc tgattcaggg cctcgaacag cggcagcagg
     1501 gcgtccaggc tgcgctgctg ctcggggcgc tcggccgtca gcgcgcgcag gcgctgccac
     1561 accggccgcg ggtccagggc agcgcccggc atgccccccg aggggggcaa ggaactcggg
     1621 ggcccggcat ctcctttgga gaccgcgcat cgcagcaatg cgggctgcgg cgcggtggcc
     1681 cgctccagtt gcgcaagatc ggcaagcact tcattgccgt gcgcgtgcag ccggtgcaat
     1741 cccagcatac gggcatggac ccaccagttg attgccagca gagcgatcgc gcccagcacc
     1801 agcaaggcat gccacagcag cacgcggcgg ccggggcgca acgcggcctt catgcgccgg
     1861 ggctttgccc gggctggccg ctgccgacgc gttgcatgtc cccccagcgc tgcttgcgct
     1921 gcgtgaccca ttgcgtgagt ccgacgatgc gccaccacgc gttgatctgg cggtagccgc
     1981 agctctcgac cacgatggcc gccagcagct gcaccaggtg gcgcggcttg ggataggtgc
     2041 gaaacgccag ctcctccagc aacagcgcgc aggcggacag caccatgccc atgcccaggg
     2101 ccagcatcat gaaggcgcca aaggccgccc aggacagcag gccggtcacc gcgagcacgg
     2161 tcatcgtcag gtagccgagc agctcaacca ccggcgcgaa gccttcgaag accagcatca
     2221 cggggcaggc cagccacccg accgcgcc
//
LOCUS       DASUCY010000209         3425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_631000, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000209 DASUCY010000000
VERSION     DASUCY010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631000"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VFV25_04020"
     CDS             <1..207
                     /locus_tag="VFV25_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEY1392513.1"
                     /translation="SVLEAANLIATVRCGREKLHFLNPVPLHEIYERWIAKFERSRLK
                     ALRDLKQHLETEGGTPPSQPRRKS"
     gene            204..662
                     /locus_tag="VFV25_04025"
     CDS             204..662
                     /locus_tag="VFV25_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY1392514.1"
                     /translation="MNANNRSSFVYVSFIRTTPEKLWAALTDPQFIRQYWFGVTVDCA
                     WKKGAPWKMAFPDGRPCDTGEILEIDPPRRMVIKWQNVWNPELMAEGPSRCTMELEPT
                     GEAVKLTITHEIDRPDAKLITAVSGGWPKVVSNLKSLLETGELVLKEFHS"
     gene            complement(719..1558)
                     /locus_tag="VFV25_04030"
     CDS             complement(719..1558)
                     /locus_tag="VFV25_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEY1392515.1"
                     /translation="MQHAADLFADATSSGPRIRVGIGGWNFAPWRNNFYPAGLVQRRE
                     LEYASRQLRAIEINGTYYGAQKPATYASWASQTPEDFVFSLKAPRYVCETKKLAEAGK
                     GVLGFIQGGLEEFGPKLGPILWQLSPWRVFDAGDLAGFLESLPRELAGWPLRHVLEVR
                     HGSFLCEDYVALARQHAIPTVFTDSDKYPSLADLTGDFVYARLMRSAADIDTGYAAQA
                     LDTWASRAKQWRDGSDIAELPHVATQQPSGARRDAFIFFISSAKERNPAAAMALQARV
                     DAG"
     gene            1638..1808
                     /locus_tag="VFV25_04035"
                     /pseudo
     CDS             1638..1808
                     /locus_tag="VFV25_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            2483..>3425
                     /locus_tag="VFV25_04040"
     CDS             2483..>3425
                     /locus_tag="VFV25_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392516.1"
                     /translation="MLAIPSSPTSVIAVAPSASSPEAMTSASRDASDVAREAQRLEAA
                     VREEAARVEAARLEAEREESARLAVAQLEARQREAARQEAERIETARLEAERQEAARQ
                     AAAQLEAQRREVARQEASRVETARLEAERQEDARQAAAQVEAQQREAARQEAERIEAA
                     RLETERQKVARQVLAQMGAQRQEAARQDAARVEAARLEAERQEAARQAAAQLEAQRQE
                     AARQETARQAAAQLEAQRQETARTDAARREAARLEAERQELARLAAERQELARVEAQQ
                     AEARREAALKAIGRQLDEEAAQRDEASTAARSPSPLPL"
BASE COUNT          623 a         1109 c         1195 g          498 t
ORIGIN      
        1 agcgtgctgg aggcggcgaa cctcattgcc accgttcggt gcggccgcga aaagctccac
       61 tttctcaacc cggtaccgct gcacgagatc tacgagcgct ggatcgccaa gttcgagagg
      121 tcgcggctca aggccctgcg cgatctgaag cagcacctcg aaacagaggg cggcacgccc
      181 ccctcgcaac cacggagaaa atcatgaacg caaacaaccg cagcagcttc gtctacgtca
      241 gcttcatccg caccacgccc gagaagttgt gggccgcgtt gaccgaccct caattcatcc
      301 gccaatactg gttcggcgtg accgtcgact gcgcttggaa gaagggcgcg ccgtggaaga
      361 tggctttccc tgacggtcgc ccctgcgaca cgggcgagat tctcgagatc gacccgccgc
      421 ggcgcatggt catcaagtgg caaaacgtgt ggaatccgga gctcatggcc gagggtccgt
      481 ctcgctgcac tatggagctg gaacccacgg gcgaggcggt caagctcacc atcacgcacg
      541 agatcgaccg gcccgacgcg aagctcatca ccgctgtgtc tggcggctgg ccgaaggtcg
      601 tttccaacct gaagtcgctg ctcgagacgg gagagttggt cctcaaagaa tttcattcat
      661 agcgaaagat caccggtcat cgttctgcgg caagaccggg acagcattcg caagccgact
      721 agccagcatc gacccgcgcc tgcagcgcca tcgccgcggc ggggttgcgc tccttggcgc
      781 tgctgatgaa gaagatgaag gcgtcgcgcc gcgcgccgga tggctgctgc gtggcaacgt
      841 gcggcagctc ggcgatgtcg ctgccgtcgc gccattgctt cgcgcgtgat gcccaggtgt
      901 ccagagcctg tgccgcatag ccggtgtcga tgtcggccgc gctgcgcatc aggcgcgcat
      961 agacgaaatc gccggtgaga tcggccagcg acgggtattt gtcggaatcg gtgaacacgg
     1021 tcggtatggc gtgctgccgt gccagcgcca catagtcctc gcacaagaag ctgccatggc
     1081 gcacctccag cacatgtctg agcggccacc cggcgagctc gcgcggcagg ctctccagaa
     1141 accctgcaag gtcgcccgca tcgaagaccc gccacggcga cagctgccac aggatggggc
     1201 cgagcttggg cccgaactcc tccagcccgc cctggatgaa gcccaacacg cccttgccgg
     1261 cctcggcgag cttcttggtc tcgcagacat agcgcggtgc cttgagcgag aacacaaagt
     1321 cctctggcgt ctgcgaagcc cagctcgcat aggtggccgg cttctgcgcg ccgtaatagg
     1381 tgccgttgat ctcgatcgca cgcagctggc ggctggcgta ttccagctcg cgccgctgca
     1441 ccagaccggc cggatagaag ttgttgcgcc agggggcgaa gttccagccg cctatgccca
     1501 cgcgtatgcg cgggcctgaa gacgtggcat cggcgaagag atcggcggca tgctgcatgg
     1561 cgtgtcggaa tcgatgcgaa ggcatgccat cttgccatgc agcgacgtta tatccctcga
     1621 tctgggggtg cgcctagccg gcaacgccgc ataccctgcg gcattgcttt gccacgcatc
     1681 tgccgcaaag cggcagcgac attcggacgg tgcaggagct gctgggtcac gccgacgtgg
     1741 cgacgatcta cacccacgtg ctgaagatgg gtgagggcgc gtcgcgcagt ccgctcgacg
     1801 cgctcccctt gcggcagcgg gcgccaacgg attcacgagg gtcgaaatgg tcgagtctga
     1861 gctgcttgtc gcggtacgac gatggccaac caccactggc gaatggcttc ggttcggccg
     1921 ccctggcggc gacccgtcgc aggcgcgatt tccgcgagcc actgggcgct ggcaatggcc
     1981 gagcccacgc atcagccgcg aacgcaggga attggctgtt gcgctgctgg cctccttgct
     2041 gattcacgcg ctgctgttga gcatgatgtt cggtggcgag ggaatcgggc ttccgggcct
     2101 ctccttcccc tggcgagatc gacggataga ggtacccgat ttgcacgttg tgctcgtgcc
     2161 ggcgttcggg gcacccgcgc agccggcggt caagtcagtc gcggagccgt cgcggctggc
     2221 atcgatcgca ccgcctgttg gcggtgggcc gcccccgact ccagccgcct cgcctgccct
     2281 gcacctgaga gacagagccg tggcgagcgt gtctacggct gagccgccgg tgcaggtcaa
     2341 ggcagagcga aatgccgtgg ccagtgcagg ccctgcgaaa gcgcctacgg gcgcgaaagg
     2401 gcctgatcgc gcggcacctc cacggatcgc ggagccggcc gccgctgccg gcgtggggtc
     2461 gtccgatgcg ccaagggcgg gcgtgctcgc cattcccagc tcgccgacgt ctgtcatcgc
     2521 ggtggcgcca agcgcttcca gcccggaggc catgacgtcg gcgtctcgag atgccagcga
     2581 tgtagcgcgg gaggcgcagc gactggaggc tgccgttcgg gaggaagccg ctcgcgtcga
     2641 ggccgcgcga ctggaggccg aacgcgagga aagcgcgcgg ctggcggtgg cccagctgga
     2701 ggcacggcag cgagaggccg cgcgtcagga agcggagcgg atcgagaccg cacgattgga
     2761 ggccgaacgc caggaagccg cgcgacaggc ggctgcacag ctggaggcac agcggcggga
     2821 ggtcgctcgc caagaagcca gtcgcgtcga gactgcgcga ctagaggccg aacgccagga
     2881 ggacgcgcga caggcggcag cccaggtgga ggcacagcag cgagaggccg cgcgtcagga
     2941 agcggagcgg atcgaggccg cacgattgga gaccgaacgc cagaaggtcg cgcggcaggt
     3001 gcttgcacag atgggggcac agcggcagga ggccgcccgc caggacgccg ctcgcgtcga
     3061 ggctgcacga ctggaggccg agcgtcaaga ggccgcgcgg caggcggcgg cacagctgga
     3121 ggcacagcgg caggaggccg ctcgccagga gaccgcgcga caagcggctg cacagctgga
     3181 ggcgcagcga caagagaccg ctcgcacgga cgcagctcgc cgcgaggcag cgcgactgga
     3241 ggccgaacgc caggagcttg cacggctggc agcggaacgg caagagctcg cgcgtgtcga
     3301 ggcccaacaa gcggaggcga ggcgagaagc ggcgcttaag gcaatcggac gtcagctcga
     3361 tgaggaagcc gcacagcgtg atgaagcctc gactgccgct cgctcgccct ccccactgcc
     3421 cctgt
//
LOCUS       DASUCY010000210         2241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1257386, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000210 DASUCY010000000
VERSION     DASUCY010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1257386"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VFV25_04045"
     CDS             <1..622
                     /locus_tag="VFV25_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEY1392517.1"
                     /translation="LAGPWIERGQIRTPVEGFSLDATPFEHRGRRYLCWAQKDPATES
                     NLYLAELADPWTLAGAPVCISRPELNWERIGFHVNEGPAVLKRHGRVLIAYSASATDA
                     NYCMGLLWADEGADLLRPASWQKSAEPVFETSEAAGQYGPGHNSFTTTSDGRTDLLVY
                     HARSYRDIVGDPLNDPNRHTRVQAFGWRADGFPDFGVPVADGPYSF"
     gene            626..>2241
                     /locus_tag="VFV25_04050"
     CDS             626..>2241
                     /locus_tag="VFV25_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 catalytic
                     domain-containing protein"
                     /protein_id="HEY1392518.1"
                     /translation="MRNLIAPLLLALPALAQALSSPNGQLQVDVSVNAERQLVYTVQR
                     AGQPVLVPSRLGLVLDGADLANGLSLVSTSAATPHHERYTLAAGKRSRVDYRANEQSV
                     TVANAQGQRLTLTLRVSDDGLAFRYTVAGSPERKRFREELTSLRFAADARAWLQPMSV
                     AKTGWKRVNPAYEEHYQMDVAAGTPSPSPAGWVFPALFRSGDTWVALTEAGLDGRFHA
                     SRLQADSSGGEYRFGLPDAREVFPGGPLLADVSGTLTTPWRVLAVGSLKTIMESTLGT
                     DLAAPAIPFPRAKIQPGHASWSWALLKDDATVFDVQKRFVDYAADMHWNYTLVDADWD
                     RKIGDAKMKELVDYARGKGVGILVWYNSSGAWNDTEYTPKGALLTRESRRREFARLKN
                     LGVKGLKIDFFGGDGASMQAYYLDLLKEAAAAGLLLNFHGATLPRGWARTYPNLMTTE
                     AVKGLEYATFDQPDEDAVARHLAMLPFARNLFDPMDFTPMVFGDIPNIQRRTRNGFEL
                     AQAVLLLSGIQHFAETPEGMATVPAGVKSLL"
BASE COUNT          364 a          839 c          703 g          335 t
ORIGIN      
        1 gctcgctggc ccctggatcg agcgcggcca gatccgcacg ccggtcgagg gcttctcgct
       61 ggacgcgaca cccttcgagc accgcggccg ccgctatctc tgctgggcac agaaggatcc
      121 ggccaccgag agcaatctct atctggccga gctcgccgat ccctggacgc tggccggtgc
      181 gccggtctgc atcagccggc ccgagctgaa ctgggagcgc atcggcttcc acgtcaacga
      241 aggcccggcc gtgctgaagc ggcatggccg cgtcctcatc gcctactccg ccagcgcgac
      301 cgacgcgaac tactgcatgg gcctgctctg ggccgatgaa ggcgccgacc tgctgcggcc
      361 cgcgagctgg caaaagtctg ccgaaccagt gttcgagacc tccgaagccg ccggccagta
      421 cggccccggc cacaacagct tcacgacgac atccgacggc cgcacagacc tgctcgtcta
      481 ccacgcgcgc agctaccgcg acatcgtcgg cgacccgctc aacgacccca accgccacac
      541 gcgcgtgcaa gccttcggct ggcgagccga tggcttcccc gacttcggcg tgccagtcgc
      601 cgatggcccc tattcgttct gaaccatgcg aaacctgatc gctcctctcc tgctcgcgct
      661 ccccgccctc gcccaggcgc tgagcagccc caatggccaa ctgcaggtcg acgtgtcggt
      721 caatgccgag cggcagctcg tctacacggt gcagcgcgcg ggccagcccg tgctggtgcc
      781 ctcgcgcctg ggcctcgtgc tcgacggtgc cgacctggcc aatggcctca gcctcgtgtc
      841 gacctcggcc gcaacgcctc accacgagcg ctacacgctg gccgccggca agcgcagccg
      901 cgtcgactac cgcgccaatg aacagagcgt cacagtcgcg aacgcccaag gccagcggct
      961 cacgctgacg ctgcgcgtgt ccgacgacgg cctcgccttt cgctacacgg tggccggcag
     1021 ccccgagcgc aagcgcttcc gcgaggagct gaccagcctg cgcttcgcgg ccgacgcgcg
     1081 cgcctggctg cagcccatgt cggtcgccaa gaccggctgg aagcgcgtga acccagctta
     1141 cgaagagcat taccagatgg acgtcgccgc aggcacgccc tcgccgtcgc cggcgggctg
     1201 ggttttccca gcgctgttca ggagcggcga cacctgggtc gcgttgacgg aggccggctt
     1261 ggacggtcgc ttccacgcgt cgcgcttgca ggccgattcg tccggcggcg aataccgctt
     1321 cggcctgccg gacgcgcgcg aggtcttccc cggcggccct ctgctcgccg acgtgagcgg
     1381 aacactcacg acgccctggc gcgtgctggc cgtgggttcg ctgaagacga tcatggaatc
     1441 gacgctcggc accgacctcg ccgcgccggc catccccttc cccagggcca agatccagcc
     1501 cggccacgcg tcctggagct gggccctgct gaaggacgat gccaccgtct tcgacgtgca
     1561 gaagcgcttc gtcgattacg ccgccgacat gcactggaac tacaccctgg tcgatgccga
     1621 ctgggaccgc aagatcggcg acgcgaagat gaaggagctg gtcgactacg cccgcggcaa
     1681 gggcgtcggc atcctcgtct ggtacaactc ctcgggtgcc tggaacgaca ccgagtacac
     1741 gcccaagggc gcgctcctga cgcgcgagtc gcgccgccgc gagttcgcgc ggctcaagaa
     1801 cctgggcgtg aagggcctca agatcgactt cttcggcggc gacggcgcct ccatgcaggc
     1861 ctattacctc gacttgctga aggaggccgc ggccgcaggc ctgctgctga acttccacgg
     1921 cgcaacgctg ccgcgcggct gggcccgcac ctaccccaac ctgatgacca ccgaggcggt
     1981 gaagggcttg gagtacgcga ccttcgatca gcccgacgaa gacgccgtcg cccgccacct
     2041 ggcgatgctg cccttcgcgc gcaacctctt cgacccgatg gatttcacgc cgatggtctt
     2101 cggcgacatc cccaacatcc agcgccgcac gcgcaacggc ttcgagctcg cacaggccgt
     2161 gctactgctg tcgggcattc agcactttgc ggaaacgccc gagggcatgg cgaccgtgcc
     2221 ggccggcgtg aaaagcctgc t
//
LOCUS       DASUCY010000211         3831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_711216, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000211 DASUCY010000000
VERSION     DASUCY010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711216"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="VFV25_04055"
     CDS             <1..548
                     /locus_tag="VFV25_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008329849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392519.1"
                     /translation="GFAVGGNKSDTSVKLAHEGGGKPWLTFTSKAAVPITQAFSSGYR
                     IAKTVTPVEQKLSGRYSRGDVLRVHLDIDAQADMTWVVINDPIPAGATLLGSGLGRDD
                     AMATGGEKEDRRGWLAYQERSLEAFRAYYRYLPKGKFSVEYTVRLNNPGAYGLPATHV
                     EAMYAPEMFGEAPNAQMVVAP"
     gene            559..2757
                     /gene="pbpC"
                     /locus_tag="VFV25_04060"
     CDS             559..2757
                     /gene="pbpC"
                     /locus_tag="VFV25_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020200485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HEY1392520.1"
                     /translation="MRCGARPFTRSLVALALGCAAHCAFAIPSFSQVKAAYRTSDTQV
                     LDRHGALLQRVRSDPTVRRLDWVTLQDVSPAFRQALLLSEDKRFYEHSGVDWSAVGAA
                     AWGNLWNTKTRGASTVTMQLAGLLDTSLAAPKGGRSIGAKLGQLQLAWQLEHGWNKDQ
                     ILEAYLNLVPFRGEVVGIGALSATVFHKIPSGLDAEEAAIAAALQRAPNARPAEVGQR
                     ACGILKAEGRKPACDMLAAMAKQWLAAPTYESFDAEEQLAPHLARRLEARAGKARPPV
                     LHTTLDAQLQRFARDSLRQHLAELRDRNVADGAVIVLDNASGDVLAWVGSSGNELSRA
                     AQVDGVTSRRQAGSTLKPFLYELAIERRWLTAASLLDDSPIDLATAAGLYVPQDYDHR
                     FKGAVSVRTALASSLNVPAVRTAVMVTPERFAERLQVLGLQLAHSGDYYGYSIALGSP
                     EVSLASLANAYRTLANGGAGSPIRTELGAVPASRTTVMDPAASFVVADILADREARTA
                     TFGLDNALSPRYWAAVKTGTSKDMRDNWCLGFSRRYTVGVWVGNANGEPMWDVSGVTG
                     AAPVWRAVMDQLENPARGIAIRPSLHSSPPSPPPGLVQAAVRFQGVPEPPRSEWFLPD
                     TQQALVSTAHATAAAASLIASPTDHSVLALDPDIPPQAQLLRIAPAGGIARNWSWRLD
                     GKRLGPAVARTWSLWPGKHRLELLDDQGRIKEISRFEVRGAELKQGRRSG"
     gene            2895..3482
                     /locus_tag="VFV25_04065"
     CDS             2895..3482
                     /locus_tag="VFV25_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEY1392521.1"
                     /translation="MGRLEAFSDGVIAIIITIMVLELKLPEHQVVNSVWADVILPMWP
                     KLLAYLWSFVLVGLMWINHHHLLHSLSSASRPLAWLNLHLLFWMSLVPATTALIGDHP
                     KLPGAVATYALVHGANTCGFLILRRYAQRLSRTGQARGHSVHTAMFLRNIAGVLMYLG
                     AAACAFLWLPLAYALIAAVPLLFLSSPAMKEEAAS"
     gene            complement(3499..>3831)
                     /locus_tag="VFV25_04070"
     CDS             complement(3499..>3831)
                     /locus_tag="VFV25_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY1392522.1"
                     /translation="DLFLTRELHHARILRAPTSPAVVDFFVEQRADVAAGVRQQLQAD
                     AQRIPGLRLLDGRFMLIQQAMGVPKRQGPQAAAFLSAFVEDAKASGFVAQALARHRIQ
                     GASVAPLG"
BASE COUNT          640 a         1313 c         1262 g          616 t
ORIGIN      
        1 ccggcttcgc agtcgggggc aacaagtccg acacgagcgt gaagctcgcc catgaaggcg
       61 gcggcaagcc ctggctcacg ttcacgagca aggccgcggt gcccatcact caagctttca
      121 gcagcggcta tcgcatcgcc aagaccgtga cgccggtcga gcagaagctg agcggtcgct
      181 acagccgagg cgacgtgctg cgcgtgcatc tcgacatcga cgcgcaggcg gacatgacct
      241 gggtggtgat caacgatccg atccccgccg gcgccacgct gctcggcagc ggcctgggcc
      301 gcgacgacgc gatggccacg ggcggagaga aggaagaccg gcgcggctgg ctggcctatc
      361 aggaacgcag tctcgaggcc ttccgcgcct actaccgcta cctgcccaag ggaaagttca
      421 gcgtcgagta cacggtgcgg ctgaacaacc ccggcgccta cggcctgccg gcgacccatg
      481 tcgaagcgat gtatgcaccc gagatgttcg gcgaggcgcc gaacgcccag atggtcgtag
      541 ccccgtaaag ttgcagccat gaggtgcggg gctcgcccct ttacgcgcag ccttgtcgcg
      601 ctggcgctgg gctgcgcggc gcactgcgcc ttcgcgatac cgagcttctc gcaggtcaag
      661 gcggcatacc gaacctcgga tacccaggtg ctcgatcgcc acggcgccct gctgcaacgc
      721 gtgcgcagcg atcccacggt gcggcgcctg gactgggtga cgcttcagga cgtgtcgccc
      781 gccttccgtc aggccctgct gctgtccgaa gacaagcgtt tctacgagca ttcgggcgtc
      841 gactggtcgg ccgtgggcgc ggcggcatgg ggcaacctgt ggaacacgaa gacgcgcggc
      901 gcatccaccg tgacgatgca gctcgcgggg ctgctcgaca ccagcttggc agcaccgaag
      961 ggcgggcgct cgatcggcgc gaagctcggg cagctccagc tcgcatggca gctcgaacac
     1021 ggctggaaca aggatcagat cctggaggcc tatctcaacc tcgtgccctt ccgtggcgag
     1081 gtggtcggca tcggcgcgct ttcggccacg gtgtttcaca agatccccag cgggctcgac
     1141 gccgaggaag ccgcgatcgc agcagcactg cagcgtgcgc cgaatgccag gcccgccgag
     1201 gtaggccaac gcgcctgcgg catcttgaag gcagaaggtc gcaagcctgc atgcgacatg
     1261 ctggctgcga tggcgaagca gtggctcgca gcgcccacct atgagtcttt cgacgccgag
     1321 gagcagcttg cgccgcacct ggcacggcgc ctggaggcgc gcgccggcaa ggctcgtcca
     1381 cccgtgttgc acaccacgct cgatgcccag ctgcaacgct tcgcgcgcga cagcttgcgg
     1441 cagcacttgg ccgagctgcg cgaccgcaac gtggcggatg gtgcggtgat cgtgctcgac
     1501 aacgcgagcg gcgacgtgct ggcctgggtg ggttccagcg gcaacgagct gtcccgggcg
     1561 gcacaggtcg acggcgtgac gagccggcgg caggccggct cgacgctgaa gcccttcctc
     1621 tacgagcttg cgatcgagcg acgctggctc accgcagcct cgctgctcga cgattccccg
     1681 atcgacctgg ccacggccgc gggcttgtac gtgccgcagg actacgacca ccgcttcaaa
     1741 ggcgcggtga gcgtgagaac ggcgctggca tcgtcgctca acgtgcctgc cgtgcgcaca
     1801 gcggtcatgg tcacgcccga gcgctttgcc gaacggctgc aagtcctggg attgcagctt
     1861 gcgcacagcg gcgactacta cggctacagc atcgcactcg gttcgcccga agtttcactg
     1921 gcttcgcttg cgaacgccta tcgcaccctc gccaatggcg gcgccggctc gccgatcagg
     1981 acagaactcg gggccgttcc cgcctcccgc acaacggtca tggacccagc cgcgagcttc
     2041 gtcgttgccg acatccttgc cgaccgggag gcgcgcaccg caaccttcgg gctggataac
     2101 gccttgtcac cgcgctactg ggccgcagtg aaaaccggca ccagcaagga catgcgcgac
     2161 aactggtgcc ttggcttctc gcggcgctac acggtgggcg tgtgggtcgg caatgcgaat
     2221 ggagaaccga tgtgggacgt atctggcgtg accggtgccg cgccggtgtg gcgggcggtg
     2281 atggatcagc tcgagaaccc ggcgcgcggc attgccatcc gcccaagcct gcactcttcg
     2341 ccgcccagcc cgccgccggg cctggtccaa gcagccgttc gctttcaagg cgtgcccgag
     2401 ccgccgcgca gcgagtggtt cctgcccgat acccaacaag cgctggtgag caccgcgcac
     2461 gcaacggcag cagcagcctc gctcatcgcc tcgccgaccg atcacagcgt cttggcgctc
     2521 gaccccgaca ttccgccgca agcccagctg ttgcgaatcg cgccagcggg agggattgct
     2581 cgcaactgga gctggcgtct cgatggcaag cgccttggcc ctgcggtggc ccgcacgtgg
     2641 tcgctgtggc ccggcaagca caggctcgag ctgctcgatg atcaaggtcg catcaaagag
     2701 atttcgcgct tcgaggtgcg cggcgccgag ctcaagcaag gtcgccggtc ggggtaggca
     2761 tcgcagtgcc aggcagtgaa acaccgcggt aggatccgct tcccgtccac ctcctcgaag
     2821 gccaagaaat gccggcatcc gacctgtcga cggcagctac aggcacagcc tcgcagcgcg
     2881 ccgctcaccc cggcatgggt cgcctggaag cgttctctga cggcgtcatc gccatcatca
     2941 tcaccatcat ggtgctcgag ctgaagctgc cggagcacca ggtcgtcaac agcgtctggg
     3001 ccgacgtcat cctcccgatg tggccgaagc ttctcgccta cttgtggagc ttcgtgctgg
     3061 tggggctcat gtggatcaac caccatcacc tgctgcattc gctgtcctca gcctcgcgcc
     3121 ccttggcctg gctcaatctg catttgctgt tctggatgtc gctcgtaccg gcaaccaccg
     3181 cgctgatcgg cgatcacccc aaactgccgg gagcggtcgc cacgtatgcc ctcgtgcacg
     3241 gcgccaacac ctgcggcttc ctcatactgc gccgctacgc ccagcgtctg tcgcgcacgg
     3301 ggcaggcgcg cgggcacagt gttcacacgg ccatgtttct gcgcaacatc gccggcgtgc
     3361 tgatgtacct gggagcagcg gcgtgcgcct tcttgtggtt gccgctggca tatgcgctga
     3421 ttgcggcggt gccgctgctg tttctgtcgt ctccggcaat gaaggaagag gcagccagct
     3481 agctagctag ctaggcaact agccaagggg tgccaccgaa gcaccctgaa tccggtgccg
     3541 ggccagcgct tgcgcgacga agccgctcgc cttggcatcc tcgacgaacg ccgagaggaa
     3601 ggccgcggcc tgcgggcctt ggcgtttcgg aacccccatc gcctgctgga tcagcatgaa
     3661 tcttccgtcc agcaggcgca ggcccgggat gcgctgcgcg tcggcctgca gctgctgcct
     3721 cacgcccgcg gccacgtcgg cgcgctgctc aacgaagaag tcgaccaccg ccggcgaggt
     3781 cggcgcccgc aggatgcgcg catggtggag ctcgcgcgtg aggaacaggt c
//
LOCUS       DASUCY010000212         2613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_116787, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000212 DASUCY010000000
VERSION     DASUCY010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116787"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VFV25_04075"
     CDS             complement(<1..382)
                     /locus_tag="VFV25_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005306.1"
                     /note="sensory histidine kinase in two-component
                     regulatory system with KdpE; signal sensing protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdbD"
                     /protein_id="HEY1392523.1"
                     /translation="MSDDSRPDPDELLAQIREDEAQAKRGRLRIYFGSSAGVGKTYAM
                     LLAARKLKDEGREVVAGIVETHGRGETQALLEGLEVLPPAQIEYRGRRLPEFDLDAAL
                     KRHPALILVDELAHSNVAGSRHPKR"
     gene            594..1784
                     /locus_tag="VFV25_04080"
     CDS             594..1784
                     /locus_tag="VFV25_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY1392524.1"
                     /translation="MTKLKKSLTTGGVAVAALALAAGVYVFATGTASSQEAQQPGNGA
                     QAQGETVALTEAQLKTVSLMTAGEQAFAVDREAVGSIDFNEDASVQVSPPYQGRVQQV
                     FVKAGDDVGKGKPLFTIESPDLVQAESTLIGSAGTLKLTSDALQRAKDLLAIQGIAQK
                     DYQQAVSDQQAAQGAHEAARNAVRIFGKSEAEIDRIVAQRRVDAQMSILSPISGRITA
                     RNIAPGVLVQPGGSPAALAVADLSTKWMLANVAESDIADIRLGQDVDVRLGAYPGRVF
                     HGRITNIGAALDPNTRRITVRSEIADPKHELQPGMFAGFTIHTGQQQRGISVPGAALV
                     REGDGGFVVWVTRDRRSFTRRVVKLGMEQGGRFQVIEGLNAGETMAGDGAIFISNAWA
                     LGTK"
     gene            1828..>2613
                     /locus_tag="VFV25_04085"
     CDS             1828..>2613
                     /locus_tag="VFV25_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004632322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY1392525.1"
                     /translation="MLKALISLALMRRPIVLLALLVFVGAGIAAYLRLNIEAYPNPAP
                     VILEITAQTPGLSAEEMERYYTVPMEIGLATTPGVDSIRSTSFYGLSFVRVTFKYGVD
                     YYFAYNQAALALQQNVSLPNNVQSQIQASSLVGEIFRYQLKGPKHFGLSNLRTLQDWV
                     VQRRFLTVPGVAQVVAWGGTTKEYEVEVDPRKLEAYHVSLPQVLTALGNANINVGGRT
                     ISLGQQSVNVRGVGLIEKVADIENVVLTQSNGVPVLVKDVAKAS"
BASE COUNT          437 a          894 c          887 g          395 t
ORIGIN      
        1 agcgcttggg gtggcgcgag ccggccacgt tggaatgggc cagctcgtcg accaggatca
       61 gggccgggtg gcgcttgagc gccgcgtcca ggtcgaactc cggcaggcgc cggccgcggt
      121 attcgatttg cgccggcggc agtacctcca ggccttcgag cagggcctgc gtctcgccgc
      181 ggccatgggt ctcgacgatg ccggccacca cctcgcggcc ctcgtccttg agcttgcgcg
      241 cggccagcag catcgcgtag gtcttgccca cgccggccga ggagccgaag tagatgcgca
      301 gccgcccgcg cttggcctgc gcctcgtcct cgcggatctg cgccagcagc tcgtccgggt
      361 cgggtcggga gtcgtcgctc atcgtggaca gcaatgtaag cccggcatcg tttgattttc
      421 gtcaagggat cgacagccgg gggcaccgag ctgcctatgg ataagcccat tgataggtga
      481 ttgcaagcct aggccgccag catggcgggg gcctggctcc tcgccggccc gcgcagccct
      541 gcgctgcttt tgcctttgct cagcgcccga gcctcgcccg ggccaccaag cctatgacca
      601 agctcaagaa aagcctgacg acaggcggtg tcgccgtcgc agccctcgcc ctggcggcgg
      661 gcgtctatgt gttcgcaacc gggaccgcat ccagccagga agcgcagcag cccggcaacg
      721 gcgcccaggc gcagggcgaa accgtggcgc tgaccgaggc ccagctcaag acggtgagcc
      781 tgatgaccgc aggcgagcag gcctttgcgg tcgatcgcga ggcggtgggc agcatcgact
      841 tcaacgagga cgcgagcgtg caggtctccc cgccctacca ggggcgggtg cagcaggtct
      901 tcgtcaaggc gggcgacgat gtgggcaagg gcaagcctct cttcacgatc gaaagcccgg
      961 acctggtgca ggccgaatcg acgctgatcg gaagcgccgg caccctcaag ctcaccagcg
     1021 atgcgctgca gcgggccaag gacctgctgg cgatccaggg catcgcgcag aaggactacc
     1081 agcaggccgt ctccgaccag caggccgccc agggcgccca tgaggccgcg cgcaatgcgg
     1141 tgcgcatctt cggcaagagc gaggccgaga tcgaccgcat cgttgcgcag cgccgcgtcg
     1201 atgcgcagat gagcatcctg agcccgatca gcgggcgcat cacggcgcgc aacatcgcgc
     1261 ccggcgtgct ggtgcagccc ggcggcagcc cggccgcgct tgcggtggcc gatttgtcaa
     1321 ccaagtggat gctggccaac gttgccgagt ccgacatcgc cgacatcagg ctcggccagg
     1381 atgtggatgt gcgcctgggc gcgtatcccg gccgggtctt ccacggccgc atcaccaaca
     1441 tcggcgcggc gctggacccc aacacgcggc gcatcacggt gcgcagcgag atcgccgacc
     1501 ccaagcacga gctgcagccg ggcatgttcg ccggcttcac gatccatacc ggccagcagc
     1561 agcgcggcat cagcgtgccc ggcgcggccc tggtgcgcga gggcgacggc ggcttcgtcg
     1621 tctgggtcac gcgcgaccgc cgcagcttca cgcgccgcgt cgtcaagctc ggcatggagc
     1681 agggcggccg gttccaggtg atcgagggcc tgaacgccgg cgagaccatg gccggcgacg
     1741 gcgccatctt catcagcaac gcctgggcgc tggggacgaa atgagccggc tgcacttccc
     1801 gattgatgtt gcagccgagg gcgaggcatg ctgaaggccc tcatttcgct ggcgctgatg
     1861 cgccggccca tcgtgctgct ggcgctgctg gtcttcgtcg gcgcgggcat cgccgcctat
     1921 ctcaggctca acatcgaggc ctatcccaac ccggcgccgg tgatcctgga gatcacggcc
     1981 cagaccccag gtctttcggc cgaggaaatg gagcgctact acacggtgcc gatggagatc
     2041 ggcctggcca ccacgccggg ggtcgacagc atacgttcga cctccttcta cggcctgtcc
     2101 ttcgtgcgcg tcaccttcaa gtacggcgtc gactactact tcgcctacaa ccaggccgcg
     2161 ctggcgctgc agcagaacgt cagcctgccc aacaacgtgc agtcgcagat ccaggcttcc
     2221 agcctggtgg gcgagatctt ccgctaccag ctcaagggcc ccaagcattt cggcctgagc
     2281 aatctgcgca cgctgcagga ctgggtcgtg cagcgccgct tcctcaccgt gccgggcgtg
     2341 gcccaggtcg tggcctgggg cggcacgacc aaggaatacg aggtcgaggt cgacccgcgc
     2401 aagctggagg cctatcacgt cagcctgccg caggtgctca cagccctggg caacgccaac
     2461 atcaatgtcg gtgggcgcac catcagcctg ggccagcaat cggtgaacgt gcgcggcgtg
     2521 ggcctgatcg agaaagtggc cgacatcgag aacgtcgtgc tgacgcagag caacggcgtg
     2581 cccgtgctcg tcaaggatgt cgccaaggcc agc
//
LOCUS       DASUCY010000213         3425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1514492, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000213 DASUCY010000000
VERSION     DASUCY010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514492"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /locus_tag="VFV25_04090"
     CDS             complement(<1..933)
                     /locus_tag="VFV25_04090"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEY1392526.1"
                     /translation="MNSVNEPGLGALDSSIQLSREPLPASSKIYEAGSIHPELRVPMR
                     AIALTNGERVTVYDASGPYTDPATDIDVRRGLPAPRAAWIAARGDTEAYEGRAIQLID
                     DGLRDQAQQLALREMSAGLQRRPRRAKAGANVTQLHYARRGLVTPEMEFIAIRENQRL
                     DWMREYEADAEREARLKGHSFGAAIPERVTPEFVRDEVARGRAIIPANINHVELEPMI
                     IGRNFLVKVNANIGNSAVSSSIEEEVEKMVWATRWGADTVMDLSTGRNIHTTRDWIVR
                     NAAVPIGTVPIYQALEKVGGVAEELSWELFRDTLI"
     regulatory      complement(1109..1234)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1352..1717
                     /locus_tag="VFV25_04095"
     CDS             1352..1717
                     /locus_tag="VFV25_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPW repeat protein"
                     /protein_id="HEY1392527.1"
                     /translation="MLQVKHWQDAVNAVLGVWLVLSPWALGFQADMTAMSTAVVIGVL
                     LVAAALGALLVPRAWEEWSEGVLGVLMIISPWVLGYSARATAMHSAVVTGIIIVALSA
                     WTLATDKDYSAWMRKREAQ"
     gene            2001..2291
                     /locus_tag="VFV25_04100"
     CDS             2001..2291
                     /locus_tag="VFV25_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020830892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEY1392528.1"
                     /translation="MATFISLLNFTDQGIRTVKESPNRAEAFTAMASKLGLTVKSIHW
                     TTGSYDLVVVTEGSDEAGVAALLSAGSLGNVRSQTLRGYSAAEFKKIISLMP"
     gene            complement(2288..3088)
                     /locus_tag="VFV25_04105"
     CDS             complement(2288..3088)
                     /locus_tag="VFV25_04105"
                     /inference="COORDINATES: protein motif:HMM:NF021437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2145 domain-containing protein"
                     /protein_id="HEY1392529.1"
                     /translation="MSAEQQDVLLRFGAIVKDELEKSGQSLVLITRSGLDLSRFGVRY
                     SHAGLSLKASRNAPWSVRQLYYACDERKPRLYDQGMSGFLLGADDPSTGYVSVVFLPR
                     EQAAPLEQAALDDTLVLQLLGAQYSANAYPFSARYQNCNQWVVELLAAAWGQPEPGQD
                     LRAQAQAWLRDQGYAPSVFNVSHTLMWLGDFVPWVHSDDHPPEDLGQLSYRVSMPASI
                     EAFVRATVPGATRMEFCHIGRRVVIRHGWDAIAEGCQPGERDRVLTLD"
     gene            complement(3220..>3425)
                     /locus_tag="VFV25_04110"
     CDS             complement(3220..>3425)
                     /locus_tag="VFV25_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392530.1"
                     /translation="GGAGKDEFYLYLPQAAYEQAKLAPGRTVTARQRPYGVEFAGGEP
                     RQAFFLALDDDWYRELQAKAVTL"
BASE COUNT          549 a         1127 c         1193 g          556 t
ORIGIN      
        1 gatcaaggtg tcgcggaaca gctcccagct cagctcctcg gccacgccgc cgaccttctc
       61 cagcgcctgg tagatcggca ccgtgccgat gggcacggcc gcgttgcgca cgatccagtc
      121 gcgcgtggtg tggatattgc ggccggtgga gaggtccata accgtgtccg cgccccaacg
      181 cgtggcccag accatcttct cgacctcttc ctcgatgctg gagctgacgg ccgaattgcc
      241 gatgttggca ttgaccttca ccaggaaatt gcggccgatg atcatcggct cgagctcgac
      301 gtggttgatg ttggccggga tgatggcgcg gccacgtgcc acctcgtcgc gcacgaactc
      361 gggcgtgacg cgttcgggaa tcgcggcgcc gaaggaatgg cctttgaggc gcgcctcgcg
      421 ctcggcgtcg gcttcgtact cgcgcatcca gtccagccgc tggttctcgc ggatcgcgat
      481 gaattccatc tcgggcgtga cgaggccgcg gcgtgcgtag tgcagctgcg tgacattggc
      541 gccggccttg gcgcggcgcg gccggcgctg cagccctgcg ctcatctcgc gcagcgcgag
      601 ctgctgcgcc tggtcgcgca ggccgtcgtc gatcagctgg atcgccctgc cctcgtaggc
      661 ctcggtgtcg ccgcgcgcgg cgatccaggc ggcgcgcggc gccggcaggc cgcgcctcac
      721 gtcgatgtcc gtcgccggat cggtgtaggg gcccgaggcg tcgtagaccg tgacgcgctc
      781 gccgttggtg agcgcgatgg cgcgcatcgg cacgcgcagc tcgggatgga tgctgccggc
      841 ctcgtaaatc ttgctcgaag caggcagggg ttcgcggctg agctggatgc tgctgtccag
      901 ggcgccgagg ccgggttcgt tgacgctgtt catggctggg tctctctccg tggcggttga
      961 agaccccgga gcctggccat gaagcggtgg cggccggcgc acgaaggctc tgagcgcgag
     1021 agaaacgggc cgagggggtg cagggggaga gatcgtgacg gcggctgccg cgacctcggc
     1081 cctggagacc tccagggccc gcacccatgg catgggagca ggctcttctt acgccggtat
     1141 caaccggatc aagttcagcg ggtttgcggc ttgtgccgca tctcagcccg acgcttgaag
     1201 aacaagcggg caacgggcgc cccggagcga gcgcgtagta taggccaagc tcggcgtggc
     1261 atgccctggt cggcgcaggc ccaaggcaga cggaacacgc gtccggcgct ccgatctaag
     1321 atttccccca tcccgacaga aggggaagac catgttgcaa gtgaagcatt ggcaagatgc
     1381 agtcaacgcc gtcctgggcg tttggctggt gctatcgccc tgggccctgg gcttccaggc
     1441 ggacatgacg gcgatgtcga ccgcagtggt catcggtgtg ctgctcgtcg ccgcggcgct
     1501 gggggcgctt ctcgtgccgc gggcctggga agaatggagc gagggcgtgc tcggcgtgct
     1561 gatgatcatt tcaccctggg tgctcggcta cagcgcgcgg gccacggcca tgcacagcgc
     1621 agtcgtcacg ggcatcatca tcgtcgcgct cagcgcttgg accttggcta ccgacaagga
     1681 ctacagcgcc tggatgcgca aacgcgaagc gcaatagccg cccatccgct ggctcaccgc
     1741 ggcggccggc gagccgcccg gtgatacgag agggttcctg ccctcatcgc gcaggcttgg
     1801 atttcggcgg acgccgccct aagctgtgca tagcgggcct taaccgctgc cgaagcggct
     1861 ggtcgcactc gtcctgcgcc ggtctgtggg agccgcagcg atgacccgcc atgtcggcga
     1921 tcgcggctgc gcttcgcccc ccaattgagg ccaggccgca gccgcggcgg cccagcctcg
     1981 catgcgagaa gggcaacacc atggccacct tcatttcgct cctcaacttc accgaccagg
     2041 gcatacgcac cgtcaaggag tccccgaacc gcgccgaggc cttcacggcg atggcctcca
     2101 agctgggcct caccgtcaag agcattcact ggaccaccgg tagctacgac ctggtggtcg
     2161 tgaccgaagg cagtgatgag gcgggagtgg cggcgctgct cagcgccggc tcattgggca
     2221 acgtgagatc tcagaccttg cgtggctact cggccgctga attcaagaaa atcatcagcc
     2281 tgatgcccta gtccagcgtg agcaccctgt cccgctcgcc gggctggcag ccttcggcga
     2341 tcgcgtccca gccgtgccgg atcacgaccc gccgcccgat gtggcagaac tccatgcggg
     2401 tggcgcccgg cacggtggcg cgaacgaagg cctcgatgga agcaggcatg ctgacgcggt
     2461 agctcagctg gccgaggtcc tcgggcggat ggtcgtcgct atgcacccag ggcacgaaat
     2521 cgcccagcca catcagcgtg tggctcacgt tgaagacgct gggcgcataa ccctgatcgc
     2581 gcagccaggc ctgggcctgg gcgcgcaggt cttgccccgg ctccggctgg ccccaggccg
     2641 cggcaagcag ctccaccacc cattggttgc agttctggta gcgcgcgctg aaggggtagg
     2701 cattggcgct gtattgcgcg cccaggagct gcagcaccag cgtgtcgtcc agcgcggcct
     2761 gctccagggg ggccgcctgc tcgcgcggca ggaagacgac cgagacatag ccggtggaag
     2821 gatcgtcggc accgaggagg aaacctgaca tgccctggtc gtagaggcga ggcttgcgct
     2881 cgtcgcaggc gtaatagagc tggcgcaccg accagggcgc attccggctg gctttgaggc
     2941 tgagcccggc gtgcgaatag cgcacgccga agcggctcag gtccaggccc gagcgggtga
     3001 tgagcaccag gctctggccg gatttttcga gctcatcctt gacgatggcg ccgaagcgca
     3061 gcaggacgtc ctgctgctcg gcactcattc gcgtctgccg gtcgcaaaag cgcagcgagc
     3121 cggcccagcc ggaaggactc cacagcaagc cacacagcag gagcagcgcc gaggcgctcg
     3181 ccgagcgaag gcctcgcccg agaccccagg ggcgcgcgct cagagcgtta cagccttggc
     3241 ttgcagctcc cgataccagt cgtcatcgag cgcgaggaag aaggcctgcc ggggctcgcc
     3301 accggcaaat tcaacgccat aggggcgctg gcgcgccgtc acggtgcgcc cgggcgccag
     3361 tttggcctgc tcgtaggcag cctggggcag gtagaggtag aactcgtcct tgccggcgcc
     3421 gcctt
//
LOCUS       DASUCY010000214         2271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1466184, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000214 DASUCY010000000
VERSION     DASUCY010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1466184"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="VFV25_04115"
     CDS             complement(<1..650)
                     /locus_tag="VFV25_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY1392531.1"
                     /translation="MSPRFLLASVAVAAWLLTIGTALAADGQDSPSPLSSAPVTRGGA
                     AAGEGYDGVVQALRQTVVAAQVPGAVVALEVKAGDRVKAGQLLLRLDARAAEQQAGAA
                     AAQVQAARAAQEAATKEFERQKQLYQKNYISQAALERAEAQYKATQAEASAQLASAGA
                     ARTQSSFYVVKAPYDGIVADVAVVLGDMAMPGRPLLTVYDPGALRVSARVPQSAAA"
     gene            794..1168
                     /locus_tag="VFV25_04120"
     CDS             794..1168
                     /locus_tag="VFV25_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY1392532.1"
                     /translation="MQGLPLEALEQVAAYFQALSEPTRLQILNLLRGGEHNVGELAQL
                     TGYTSANVSRHLALLVQHGLLERESRGTAVYYRISDASVYELCDLVCGSIARRLEQAA
                     SGRQAFTRSAATAAAKPKQRRA"
     gene            1226..1612
                     /locus_tag="VFV25_04125"
     CDS             1226..1612
                     /locus_tag="VFV25_04125"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HEY1392533.1"
                     /translation="MDPATQQHLTALRQLLKQRQRELGADVHAADLERQQQEPVGLHE
                     VADQKDGATQLQMAEADSAQKQLERNELAEVEAALRRLDKGAYGDCTDCGGPIPLQRL
                     MVQPAALRCAACQTARERDREHKQPR"
     gene            1716..>2271
                     /locus_tag="VFV25_04130"
     CDS             1716..>2271
                     /locus_tag="VFV25_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY1392534.1"
                     /translation="MNNNKPDPSALPDVIRSLPELLAWRVAATPQAEAYRQFDAQRKS
                     WVSWSWAAFGERVALWSRALGRLALPRGARVAILLPNSMDAVSIDQAVLAQACVPVPL
                     HAIDNPASIAYILADSEASVLVASTLEQWQAIAALGQPLPQLREVVLRDLAPGQTVES
                     APVPVPVLALADWLARAEAAAPAAP"
BASE COUNT          357 a          805 c          773 g          336 t
ORIGIN      
        1 ttcgcggccg cgctttgcgg cacccgtgcg ctgacgcgca gcgcgccggg gtcgtagaca
       61 gtgagcagcg gacgccccgg catcgccata tcgcccagca ccaccgccac atcggcgacg
      121 atgccgtcgt agggagcctt caccacgtag aagctcgact gggtgcgcgc cgcgcctgcg
      181 ctggcgagct gcgccgaggc ctcagcctgc gtcgccttgt actgcgcctc ggcccgctcc
      241 agcgcggcct ggctgatgta gttcttctgg tagagctgtt tctggcgctc gaactccttg
      301 gtagccgcct cctgcgcggc ccgcgcggcc tgcacctgag cagcggcggc gccggcttgc
      361 tgctcggcgg cgcgcgcatc caggcgcagc agcagctgcc cggccttcac ccggtcgccg
      421 gccttgacct cgagtgccac cacggcgccg ggcacctggg cggcgacgac cgtctggcgc
      481 agcgcctgca cgacgccgtc atagccttcg ccggcagcgg ctccgccgcg cgtcacgggc
      541 gcactcgaaa gcgggcttgg ggagtcctgc ccgtcggcgg caagcgcggt gccgatcgtc
      601 agcaaccaag ccgcgaccgc gacggaagca aggaggaatc gggggctcat gttgcagctt
      661 gcagtggctt gtcgttaatg tattatttaa ctaaatagct aataatgcaa gtatggattt
      721 gagccgcaga tgcaacgaag gcgcaccgat gaggcatgat ccaggctcgt acccggtttc
      781 ctgtcgttcc agcatgcaag gcttgcctct cgaagccctc gaacaagtcg ccgcctattt
      841 ccaggcgttg tcggagccga cgcgcctgca gatcctcaac ctgctgcgcg gcggagagca
      901 caacgtgggc gagctggcgc agctcacggg ctacacctcc gccaacgtgt cgcgccacct
      961 ggcgctgctg gtgcagcacg ggctgctcga gcgtgaaagc cgcggcaccg cggtctacta
     1021 ccgcatctcc gacgcctcgg tctacgagtt gtgcgacctg gtgtgcggca gcatcgcccg
     1081 ccgcctcgag caggctgcca gcgggcggca ggccttcacg cgcagcgctg ccaccgcagc
     1141 cgccaagccg aagcagcggc gcgcttgatc cggcgcggtg cgatgccata cagtggcatg
     1201 aacattgaac cgcttgggat tcaagatgga tccggcaacc cagcaacacc tgacagcgct
     1261 tcgccagctg ctgaagcaac ggcagcggga gctcggcgcc gacgtgcatg cggcggacct
     1321 tgagcggcaa cagcaggagc ccgtgggcct gcatgaggtt gccgaccaga aggatggggc
     1381 gacgcagctg cagatggccg aggcagacag tgcgcagaag caactagagc gcaacgagct
     1441 ggcagaagtc gaggcggctt tgcggcgcct ggacaagggc gcctacggcg actgcaccga
     1501 ttgcggaggg cccatcccgc tgcagcgctt gatggtgcag ccggccgcgc tgcgttgtgc
     1561 ggcctgccag acggcccgcg agcgggatcg cgagcacaag cagccccgtt gatgagacgg
     1621 ggcggccccc tcaacggccg tgggctgcca gggtgcgatg gccacgcgta gcccgttgcg
     1681 gtccgccccc gcgcgacaag gcatcattcg cacccatgaa caacaacaag ccagacccct
     1741 ccgcgcttcc cgacgtcatc cgcagcttgc ccgagctgct cgcctggcgc gtcgccgcca
     1801 cgccccaggc cgaggcctac cggcagttcg atgcccagcg caagagctgg gtgagctgga
     1861 gctgggccgc cttcggcgag cgcgtcgcgc tttggagccg ggcgcttggg cggctggcac
     1921 tgccgcgcgg cgcccgcgtc gccatcttgc tgcccaacag catggacgcc gtgagcatcg
     1981 accaggccgt gctggcccag gcctgcgtgc cggtccccct ccatgccatc gacaacccgg
     2041 ccagcatcgc ctacatcctg gccgacagcg aggcctcggt gctcgtggcc tccacgctgg
     2101 agcagtggca ggccatcgcc gccttggggc agccattgcc gcagctgcgc gaagtcgtgc
     2161 tgcgcgacct ggcgccgggg cagaccgtgg aaagtgcgcc cgtgcccgtg cccgtgctcg
     2221 cgctggccga ctggctggca cgcgccgagg cggcagcgcc ggccgccccc g
//
LOCUS       DASUCY010000215         3833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1177072, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000215 DASUCY010000000
VERSION     DASUCY010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177072"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1795
                     /gene="pepN"
                     /locus_tag="VFV25_04135"
     CDS             <1..1795
                     /gene="pepN"
                     /locus_tag="VFV25_04135"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEY1392535.1"
                     /translation="GHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSMDLAGSAS
                     ARAVKRIDDVRGLRQMQFPEDAGPMAHPVRPDSYQAIDNFYTATVYEKGAEVVRMQHT
                     LVGRDGFAKGMALYFQRHDGQAVTCDDFAQAIADANPGSELATRLDQFKRWYAQAGTP
                     HVKAVGRYDAHSRSYTLLLEQSCAPSPGQPDKQPYVIPVAMGLVSRDGQALPLQLEGE
                     ANSAGTSRVLVLSEARQFYTFVNLDSEPVPSLLRGFSAPVVLDAALPDADLITLLAHD
                     SDAFNRWEAGQRLALNRMLAAVQGDGQVALDAGFVEAMRGVLRHPELDAAFKELVLTL
                     PSERYVAEQLAVVDPQRIHAVRETMNLQLAQALREDWLWAWEAHQVAGGYTPDAVSSG
                     KRALANLALANLVLDAVARGDAVWPGKALQRFKDAGNMTERMGALSALVNAQAELAAP
                     ALERFHAMFKDEALVIDKWFALQAGASEPLGDKAGAVFARAKALTQHPDFSLRNPNRA
                     RSLLMSLCTNNPAAFHRADAAGYVFWADKVIEIDAINPQLASRLARALDRWTQLAEPY
                     RSAAREAISRVAAKAELSPDVREIVTRALQD"
     gene            1816..2826
                     /locus_tag="VFV25_04140"
     CDS             1816..2826
                     /locus_tag="VFV25_04140"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEY1392536.1"
                     /translation="MTRRVSLTQYLVEQQREHGRIPSQLRLLLEVVARACKQIAISVN
                     KGALGDVLGSAHTENVQGEVQKKLDIIANEVLIEANEWGGALAAMASEEMDSIHVIPN
                     RYPQGEYLLLFDPLDGSSNIDVNVSIGTIFSVLRKVGHHHGVSEDDFLQPGKAQAAAG
                     YCVYGPQTVLVLTVGDGVAMFTLDREQGSWVLTSENMRVPEDTKEFAVNMSNMRHWAP
                     PVKRYIDECLAGKEGPRGKDFNMRWIASMVADVHRILTRGGVFMYPWDQREPDKPGKL
                     RLMYEANPMAFIIEQAGGAATTGAQRIMELQPTKLHERVSVVLGSKNEVERVTAYHHE
                     AA"
     gene            complement(2933..3301)
                     /locus_tag="VFV25_04145"
     CDS             complement(2933..3301)
                     /locus_tag="VFV25_04145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY1392537.1"
                     /translation="MNARTTAAALPRNAALALGMVAAMLLAVVPHKAQADGAPKIIKK
                     VAPDFPPEAARKHITDGVVKARLNIDGAGAVTDVSVTQVSPSNAKVFNEAAVSALSQW
                     RFEPSGKGQSAEITLVFQMD"
     gene            complement(3333..>3833)
                     /locus_tag="VFV25_04150"
     CDS             complement(3333..>3833)
                     /locus_tag="VFV25_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY1392538.1"
                     /translation="RIKDIIAVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAGEV
                     RSLAARAGAAAKEIKALIDDSADKVADGTRTVADVGQRIRGIVAEVVGVRQMIEDLSH
                     ASAQQESGVLAINTSVADLDQSTQQNAALVEELAASTESLRMNARKLVSTVDFFRLAD
                     ASLAAA"
BASE COUNT          632 a         1350 c         1289 g          562 t
ORIGIN      
        1 gggccacgag tatttccaca actggaccgg caaccgcgtc acctgccgcg actggttcca
       61 gctctcgctg aaggaaggcc tcaccgtctt ccgcgaccag gaattcagca tggacctggc
      121 cggctcggcc agcgcccgcg cagtcaagcg catcgacgac gtgcgcggcc tgcgccagat
      181 gcagttcccc gaggatgccg gccccatggc ccacccggtg cgcccggaca gctaccaggc
      241 catcgacaac ttctacaccg ccacggtcta cgagaagggg gccgaggtgg tgcgcatgca
      301 gcacacgctg gtcggccgcg acggcttcgc caagggcatg gccctgtact tccagcgcca
      361 cgacggccag gccgtgacct gcgacgattt cgcgcaggcg atcgccgatg ccaacccggg
      421 cagcgaactc gccacgcgcc tggaccagtt caagcgctgg tatgcgcagg ctggcacgcc
      481 gcatgtgaag gccgtgggcc gctacgacgc gcacagccgc agctacacgc tgctgctgga
      541 gcaaagctgc gcaccctcgc ccggccagcc ggacaagcag ccctatgtga tccctgtcgc
      601 gatggggctg gtctcgcgcg acggccaggc gctgccgctg cagctcgaag gcgaggccaa
      661 cagcgccgga accagccgcg tgctggtgct gagcgaggcg cgccagttct acaccttcgt
      721 caacctcgac agcgagcccg tgccctcgct gctgcgcggc ttctcggcac cggtggtgct
      781 ggacgcggcg ctgcccgatg ccgacctcat caccctgctg gcccacgaca gcgacgcctt
      841 caaccgctgg gaggcgggcc agcgcctggc gctgaaccgc atgctcgcgg cggtgcaagg
      901 cgatggccag gtcgcgctcg acgctggctt cgtcgaggcg atgcgcggcg tgctgcgcca
      961 tcccgagctc gacgcggcct tcaaggagct ggtgctgacg ctgccgagcg agcgctacgt
     1021 cgccgagcag ctggccgtgg tcgacccgca gcgcatccac gccgtgcgcg agacgatgaa
     1081 cctgcagctg gcccaggccc tgcgcgaaga ctggctctgg gcctgggagg cgcaccaggt
     1141 ggcgggcggc tacacgcccg atgcggtgtc ttcgggcaag cgcgcgctgg ccaacctcgc
     1201 gctggccaac ctggtgctgg atgcggtggc gcgcggcgat gccgtatggc ccggcaaggc
     1261 gctgcagcgc ttcaaggacg ctggcaatat gaccgagcgc atgggcgccc tgtcggcgct
     1321 cgtcaacgcc caggccgagc tggccgcgcc ggccctggag cgcttccacg cgatgttcaa
     1381 ggacgaggcg ctggtgatcg acaagtggtt cgcattgcag gccggcgcca gcgagccgct
     1441 gggcgacaag gccggcgcgg tgttcgcccg cgccaaggcg ctgacccagc atccggactt
     1501 ctcgctgcgc aaccccaacc gcgcgcgcag cctgctgatg agcctgtgca ccaacaaccc
     1561 ggccgccttc caccgcgccg acgcggcggg ctatgtgttc tgggccgaca aggtgatcga
     1621 gatcgacgcg atcaacccgc agctcgcctc ccgcctggcc cgcgcgctgg accgctggac
     1681 gcagctggcc gagccctatc gcagcgccgc ccgcgaagcc atcagccgcg tggcggccaa
     1741 ggccgagctg tcgcccgacg ttcgcgagat cgtcacccga gcactgcagg attgaacaac
     1801 acccaaggaa gccgcatgac acgccgagtc agcctgaccc aatacctcgt cgaacagcag
     1861 cgcgagcatg gccgcatccc cagccagctg aggctgctgc tcgaggtggt ggcgcgcgcc
     1921 tgcaagcaga tcgccatcag cgtcaacaag ggcgccctgg gcgacgtgct gggcagcgcc
     1981 cacaccgaga acgtgcaggg cgaggtgcag aagaagctcg acatcatcgc caacgaggtg
     2041 ctgatcgagg ccaacgaatg gggcggcgcg ctggccgcga tggcctcgga ggagatggac
     2101 agcatccacg tcatccccaa ccgctacccc cagggcgaat acttgctgct gttcgacccg
     2161 ctggacggct ccagcaacat cgacgtcaac gtcagcatcg gcaccatctt ctcggtgctg
     2221 cgcaaggtgg gccaccacca tggcgtgagc gaagatgatt tcctgcagcc cggcaaggcc
     2281 caagccgcgg caggctattg cgtttacggc cctcagaccg tgctggtgct cacggtgggc
     2341 gacggcgtcg cgatgttcac gctcgaccgc gagcagggct cctgggtgct caccagcgag
     2401 aacatgcgcg tccccgagga caccaaggaa ttcgcggtca acatgagcaa catgcgccac
     2461 tgggcgccgc ccgtgaaacg ctacatcgac gaatgcctag ccggcaagga aggcccgcgc
     2521 ggcaaggact tcaacatgcg ctggatcgcg tcgatggtgg ccgacgtgca ccgcatcctc
     2581 acgcgcggcg gcgtcttcat gtacccctgg gaccagcgcg agcccgacaa gcccggcaag
     2641 ctgcgactga tgtacgaggc caaccccatg gccttcatca tcgagcaggc cggcggcgcg
     2701 gccaccaccg gcgcgcagcg catcatggaa ctgcagccga ccaagctgca cgagcgcgtc
     2761 agcgtggtgc tgggctcgaa gaacgaggtc gagcgcgtga ctgcctatca ccacgaggcc
     2821 gcctgagatt tccgcgtccc ccagggacat tgccaagcag gcgccgcggc tccgccggtc
     2881 cgctggcgtc gcccccttga ggggggagcg cgtcagcgct acgcgggtgg gcctaatcca
     2941 tctggaacac gagcgtgatc tcggcgctct ggcccttgcc ggagggctcg aagcgccatt
     3001 ggctcagcgc cgacacggcg gcctcgttga agaccttcgc gttcgaaggc gagacctgcg
     3061 tcacgctcac gtcggtgacg gcacctgcgc cgtcgatgtt cagccgcgct ttcaccacgc
     3121 catcggtgat gtgcttgcgt gccgcctccg gcgggaagtc cggcgcaacc ttcttgatga
     3181 tcttgggggc gccgtcggcc tgcgccttgt gcggcacgac cgccagcagc attgcggcca
     3241 ccatgcccag ggcgagggct gcgttgcggg gcagcgctgc ggcggtggta cgagcgttca
     3301 tgggctactc cttctagacg ggaaactgaa gttcaggcgg cggcgagcga cgcatcggcc
     3361 agccggaaga aatcgacggt ggacaccagc ttcctggcgt tcatcctgag cgactcggtg
     3421 ctggcggcga gctcttcgac cagcgccgcg ttctgctgcg tgctctgatc gagatcggcc
     3481 acgctggtgt tgatggccag cacgccgctt tcctgctgcg cgctcgcgtg cgagaggtcc
     3541 tcgatcatct ggcgcacgcc gacgacctcg gccacgatgc cgcgaatgcg ctggcccacg
     3601 tcggccacgg tacgggtgcc gtcggccacc ttgtcggccg agtcgtcgat cagcgccttg
     3661 atctccttgg cggcggcgcc ggcccgcgcg gccagcgagc gcacctcgcc ggccaccacg
     3721 gcgaagccgc ggccctgctc gcccgcgcgt gccgcctcga ccgccgcgtt gagcgccagg
     3781 atgttggtct ggaaggcgat gccgtcgatg acggcgatga tgtccttgat gcg
//
LOCUS       DASUCY010000216         3093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_357831, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000216 DASUCY010000000
VERSION     DASUCY010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357831"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1676)
                     /locus_tag="VFV25_04155"
     CDS             complement(<1..1676)
                     /locus_tag="VFV25_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY1392539.1"
                     /translation="MFLFAAAASLGTALLLSGVPACAQSGGATKVGDTIVLDARDALR
                     RQDKARLAAARTAIAATQHPLAMWVDYWELNNRLSEASQDELDAFYARWPGTYVEDRL
                     RNDWLLELGKRRDWRNLAREYPRFRMNDDREVSCYALLARHFDGEDVRTQARAAWLAQ
                     READDGCALLASTLYAAGRFTPADVWRKARLATEANRQKVATQAAGLIGDDAAKALAK
                     IFDDPLRYLARTRPRERNGYELAALAVLRLAGNDPQAAATQLDEPWGARLRRELGPPD
                     LAWIWASVGKQAALKLAPEADAYYQRAAQVRPGAVVDLPDDTLAWQARTALRADNAAD
                     HWPRVLQAIAALSPAEQREPTWVYWKARALQAMDPPPAASTAASAAPVAASGAAAPAA
                     SIAAAAAASGASDAQPSAPKPTPAQLLLESIANQYSFYGKLAAEELGRPFAAPPKPAP
                     LTPQELTQARATPGLARSLQLIALGLRNEGVREWNYTLRGMGDRELLAAAQIACEAQV
                     WDRCINTSDRTRSEIDMAQRFPTPYREDVVRAASESGIDPAYLYGLIRQES"
     gene            2013..2195
                     /locus_tag="VFV25_04160"
     CDS             2013..2195
                     /locus_tag="VFV25_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392540.1"
                     /translation="MNSTNRMNRIIAALFAVLMTSLTLTLAIAPAVNTPRYVDAPAGL
                     QVVELPAVVITSSHRG"
     gene            complement(2281..>3093)
                     /locus_tag="VFV25_04165"
     CDS             complement(2281..>3093)
                     /locus_tag="VFV25_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY1392541.1"
                     /translation="VPALDRLDASVRQIRQARGRHVVNVTTFASFASLWLLPRLEAFQ
                     REHPDIDIRVAANDRLTALDDTEMDLILRYTSPLEVPDDAVHLFGETITPAISPWLAA
                     RIAAGEAPPLKRPADLIQHTLLEEDDHRPSAEYLGWRHWLAQQGEAQLEPRSWIYLNF
                     TYQQVQAALSGQGVALARIGLVAEQLQRGELVEPFGHDGRIHSPYAYWLILLAHSRER
                     PEVLEFAAWVQAQAALTRAEMGEMVGTEAGSVASKGRAGKKKTGRHAGGVTP"
BASE COUNT          450 a         1061 c         1053 g          529 t
ORIGIN      
        1 gactcctgcc ggatcaggcc gtagagatag gccgggtcga tgccgctctc gctggctgcg
       61 cgcaccacgt cctcgcgata gggcgtgggg aagcgctgcg ccatgtcgat ctcgctgcgg
      121 gtgcggtcgc tggtgttgat gcagcggtcc cagacctggg cttcgcaggc gatctgcgcc
      181 gcggcgagca gctcgcggtc tcccatgccg cgcagcgtgt agttccattc gcgcacgccc
      241 tcgttgcgca ggcccagggc gatcagctgc agcgagcgcg caaggcctgg cgtggcgcgc
      301 gcctgggtca gctcctgcgg cgtcagcggc gcgggcttgg gcggcgcggc gaaaggccgc
      361 cctagttcct cggccgcgag cttgccgtag aagctgtact gattggcgat ggactccagc
      421 agcaactggg ccggcgtggg cttcggcgcg ctgggctgtg cgtcgctggc gccgcttgcc
      481 gctgcggcag cagcgatgct cgcagccggc gcagcggcac cgctcgccgc aacaggggca
      541 gcgcttgccg ccgtgcttgc ggccggcggc ggatccatgg cctgcaacgc acgcgccttc
      601 caatagaccc aggtcggctc gcgctgctct gcagggctca aggcggcaat ggcctgcagc
      661 acgcgcggcc agtggtcggc ggcgttgtcg gcgcgcagcg cggtgcgcgc ctgccaggcc
      721 agggtgtcgt cgggcagatc gaccactgcg ccgggcctca cctgggcggc gcgctggtag
      781 taagcgtcgg cctcgggcgc gagcttgagg gccgcctgct tgccgacgct ggcccagatc
      841 caggccaggt ctgggggccc cagttcgcgc cgcaaacgcg cgccccaggg ttcgtccagc
      901 tgcgtcgcgg cggcctgtgg atcgttgccg gccagccgca gcacggccag cgccgcgagt
      961 tcgtagccgt tgcgctcgcg cggtctggtg cgtgccagat agcgcagcgg gtcgtcgaat
     1021 atcttggcga gcgccttggc agcgtcatcg ccgatcaggc ccgcggcctg agtcgccacc
     1081 ttctggcgat tggcctcggt cgccagacgg gccttgcgcc agacatcggc cggcgtgaag
     1141 cggcccgccg catagagcgt gctggcgagc agcgcgcagc cgtcgtccgc ttcgcgctgg
     1201 gccagccagg cggcgcgcgc ctgggtgcgc acgtcttctc cgtcgaagtg gcgcgccagc
     1261 agtgcatagc agctcacctc gcggtcgtcg ttcatgcgaa agcgcgggta ctcgcgggcc
     1321 aggttgcgcc agtcgcgacg cttgcccagc tccagcaacc aatcgttgcg caagcggtct
     1381 tcgacatagg taccgggcca gcgcgcgtag aaggcatcga gctcgtcctg gctcgcttcg
     1441 ctcaagcgat tgttgagctc ccaatagtcc acccacattg caagcggatg ctgggtggct
     1501 gcgatcgcgg tgcgcgctgc ggccagcctg gccttgtcct gccggcgcag cgcatccctg
     1561 gcgtcgagca cgatggtgtc gccgaccttg gtcgcgccgc cgctctgtgc acaggctggt
     1621 acgccgctaa gcagcagcgc cgtacccagg cttgccgcgg cggcgaacaa gaacatgggc
     1681 cgatggaaag ctgccgagcg cagcctggga aacgtcaaga acctcgcgaa cggttctcca
     1741 tatcttcgaa cgcggcaaaa caacttcttc gtcatgcgtg gaatcccccc tgctgcagcg
     1801 cctgaccaac cggcgcccca gggtctggtc tgaatttaac ccagtggttc ccccgacgcc
     1861 cagcaatgtc cctggccact catccgcttg ccgcgcagtt caagccgctg ccgacgctgt
     1921 tcgtcacatc gcgtttgagg cctgcggcgc gcctgcctag agttcagcca tcgcaagccg
     1981 caaggctcgc tgcacaaacg aaaggaaaca tcatgaacag cacgaaccgc atgaatcgca
     2041 tcattgccgc cctgttcgcg gtgctgatga cctccctcac gctgaccctg gccatcgccc
     2101 ccgcagtgaa cacgccgcgc tatgtggatg cccccgcagg cctgcaggtg gtcgagctgc
     2161 ccgcggttgt catcacctcg tcacatcgcg gctgattttt ctgcgcgcag cgagcccctc
     2221 actctccgtc tttcatcagg ccctcgacga cagcaagact ccctagcgtc gagtgttttt
     2281 tcagggtgtc acgcctccgg catgacgccc tgttttcttt ttgccggccc ggcccttgct
     2341 ggcgacgcta ccggcctctg taccgaccat ttcacccatc tcggcgcgcg tcaatgcagc
     2401 ctgcgcctgc acccaggccg cgaattcgag cacctcgggg cgctcgcggc tgtgggcgag
     2461 caggattagc caataggcat aggggctgtg gatgcggccg tcgtggccga agggttcgac
     2521 cagttctccg cgctgcaatt gctcggccac caagccgatg cgcgccagcg cgacgccttg
     2581 gcccgacagc gcggcctgca cctgctgata ggtgaagttc agatagatcc agctgcgcgg
     2641 ctcgagctgg gcctcgccct gctgggccag ccaatggcgc cagccgaggt attcggcgct
     2701 ggggcgatga tcgtcctctt ccagcagcgt gtgctggatc aggtcggcgg ggcgcttcag
     2761 cggcggcgcc tcgccggcgg cgatcctggc ggccagccag ggactgatgg ccggcgtgat
     2821 ggtctcgccg aagagatgca cggcgtcgtc cggcacttcg agcgggctgg tgtagcgcag
     2881 gatcaggtcc atctcggtgt cgtccagcgc ggtgaggcga tcgttggcgg cgacacggat
     2941 gtcgatgtcg ggatgctcgc gctgaaaggc ttccagccgc ggcagcagcc acagcgaagc
     3001 gaaggaggcg aaggtcgtca cgttgaccac atgccgcccg cgcgcctggc gtatctggcg
     3061 caccgaggca tcgagcctgt ccagcgccgg cac
//
LOCUS       DASUCY010000217         3045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1402029, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000217 DASUCY010000000
VERSION     DASUCY010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402029"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1523
                     /locus_tag="VFV25_04170"
     CDS             <1..1523
                     /locus_tag="VFV25_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEY1392542.1"
                     /translation="TAENSWGYHWLPKWDTGYDVLRAFEMMNQGKLNGYICQGFNPVG
                     SFPNKKKIVAGLSKLKYLVIIDPLNTETAEFWKNHGEYNDVKSEEIQTTVFRLPSTCF
                     AEEDGALTNSGRWLQWHWKGAPPPGEAKGDAEIMAEIYTRLRKAYAKDGGAFPDPIVN
                     LSWPYKIPNSPSPEELAMEYNGKALADLTDPKDPTKLLAKAGEQLSGFGLLRDDGSTS
                     SGCWIYAGAWTQAGNQMARRDNSDPWGIGQTLNWAWAWPANRRILYNAASSDPTGKPW
                     RADRRLVQWNGSAWGGADVPDIRPDANPGDADRVQPFIMTAEGVARLFAPTGMAEGPL
                     PEHYEPFESPLAGNLMHPNNTKAKSNPAARVFKGDMEAFGTPKDFPYVATSYRLTEHF
                     HYWTKNVMTSAILQPQQFVEIGEELAKEKGIANGDMVKVSSNRGYIKAVALVSKRIPA
                     LQVDGKTTHTVGLPNHWGFVGLAKPGYLVNTLTPFVGDANTQTPEYKSFIVNIEKA"
     gene            1534..2403
                     /gene="fdxH"
                     /locus_tag="VFV25_04175"
     CDS             1534..2403
                     /gene="fdxH"
                     /locus_tag="VFV25_04175"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0045333 - cellular respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HEY1392543.1"
                     /translation="MSSLQSLDIALRSATTTPPPQARRTPEVAKLIDVTSCIGCKACQ
                     VACMQWNDLRDEVGDNVGVYDNPSDLTPESWTVMRFSEVEPEPGRLEWLIRKDGCMHC
                     DDPGCLKACPAPGAIVKYSNGIVDFISEHCIGCGNCVAGCPFDVPRISKKDSKAYKCS
                     LCSDRVGVGLEPACVKACPTGAIRFGTKEDMLEFGAERLVDLKERGYANAAVYDPPGV
                     GGTHVVYVLQHGDKPELYHGLPANPRISPLVALWKGVAKPLAVFAMVGAVVAGFMHYM
                     KVGPVETKEDERV"
     gene            2414..3043
                     /locus_tag="VFV25_04180"
     CDS             2414..3043
                     /locus_tag="VFV25_04180"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HEY1392544.1"
                     /translation="MSQEPRYLQRYDDSGRMLHWSVALLFFCAALSGLALFHPALYFF
                     SALFGGGPWTRILHPYIGVLMALSFAVIAARLWRLNLIEQNDRDWLAHVGDVLKGDKD
                     KTPPVGRYNGGQKVLFWLMVACLAVLFVTGFVFWRPWFAPYFPITLIRIAVLLHAMAA
                     TLLILGIIVHIYAAIWVKGTLRAMTRGSVSEAWAKQNHPLWHREMTQGK"
BASE COUNT          553 a         1036 c          991 g          465 t
ORIGIN      
        1 caacggccga gaacagctgg ggctaccact ggctgcccaa gtgggacacc ggctacgacg
       61 tgctgcgcgc cttcgagatg atgaaccagg gcaagctcaa cggttacatc tgccagggct
      121 tcaacccggt gggctccttc ccgaacaaga agaagatcgt cgcgggcctg tccaagctca
      181 aatacctcgt catcatcgac ccgctcaaca ccgagacggc ggagttctgg aagaaccacg
      241 gcgagtacaa cgacgtcaag tccgaggaga tccagaccac ggtgttccgg ctgccgtcga
      301 cctgctttgc cgaggaagac ggcgcgctca ccaactccgg ccgctggctg cagtggcact
      361 ggaagggcgc gccgccgccc ggcgaggcca agggcgatgc cgagatcatg gccgagatct
      421 acacgcggct gcgcaaggcc tatgcgaagg acggaggcgc cttccccgac cccatcgtca
      481 acctcagctg gccctacaag attcccaaca gcccctcgcc cgaggagctg gcgatggaat
      541 acaacggcaa ggcgctggcc gacctcaccg acccgaagga tccgaccaag ctgctggcca
      601 aggccggcga gcagctctcc ggcttcggcc tgctgcgcga cgacggcagc accagcagcg
      661 gctgctggat ctacgccggc gcctggacgc aggccggcaa ccagatggcc cgccgcgaca
      721 acagcgaccc gtggggcatc ggccagacgc tgaactgggc gtgggcgtgg ccggccaacc
      781 ggcgcatcct ctacaacgcc gcgtccagcg accccaccgg caagccctgg cgggccgacc
      841 ggcggctggt gcagtggaac ggcagcgcct ggggcggcgc cgacgtgccc gacatccggc
      901 ccgacgcgaa ccccggcgat gccgaccgcg tgcagccttt catcatgact gccgagggcg
      961 tggcgcgcct cttcgcgccc accggcatgg ccgaggggcc gctgcccgag cactacgagc
     1021 cctttgagtc gccgctggca ggcaacctga tgcaccccaa caacactaag gccaagtcca
     1081 atccggcggc ccgggtgttc aagggcgaca tggaggcctt cggcacgccg aaagacttcc
     1141 cgtacgtggc cacgagctat cggctcaccg agcacttcca ctactggacc aagaacgtca
     1201 tgacctcggc catcctccag ccgcagcagt tcgtcgagat cggcgaggaa ctggccaagg
     1261 agaaggggat cgccaacggc gacatggtga aggtcagctc caaccgcggc tacatcaagg
     1321 ccgtggcgct ggtgagcaag cgcatcccgg cgctgcaggt ggacggcaag acgacgcaca
     1381 cggtgggcct gcccaaccac tggggcttcg tgggcctggc caagcccggc tacctcgtga
     1441 acacgctgac gcccttcgtc ggcgacgcga acacgcagac gccggagtac aagtcgttta
     1501 tcgtcaacat cgagaaggct tgaggagcac gccatgtctt cactccagtc tctcgatatc
     1561 gcgctgcgct cggccaccac cacgccgcca ccccaggcgc gcagaacgcc cgaggtggcc
     1621 aagctgatcg acgtgacgag ctgcatcggc tgcaaggcct gccaggtcgc ctgcatgcag
     1681 tggaacgatc tgcgcgacga ggtgggcgac aacgtcggcg tctacgacaa cccgagcgat
     1741 ttgacgcccg agtcctggac ggtgatgcgc ttctccgaag tggagcctga gcccggccgg
     1801 ctcgaatggc tgatccgcaa ggacggctgc atgcactgcg acgatccggg ctgcctcaag
     1861 gcctgcccgg cgcccggcgc gatcgtcaag tacagcaacg gcatcgtcga cttcatcagc
     1921 gagcactgca taggctgcgg caactgcgtc gccggctgtc ccttcgacgt gccgcgcatc
     1981 agcaagaagg acagcaaagc ctacaagtgc tcgctgtgct ccgaccgcgt gggcgtcggc
     2041 ctggagccgg cctgcgtcaa ggcctgcccg accggcgcga tccgcttcgg caccaaggaa
     2101 gacatgctgg agttcggcgc cgagcgcctc gtcgacctca aggagcgcgg ctatgccaac
     2161 gcggcggtct acgacccgcc cggcgtcggc ggcacgcacg tggtctatgt gctgcagcac
     2221 ggcgacaagc ccgagctcta tcacggcctg ccggccaacc cgcgcatcag cccgctggtc
     2281 gcgctgtgga agggcgtggc caagccgctg gccgtgttcg cgatggtggg ggcggtcgtg
     2341 gccggcttta tgcactacat gaaggtcggg ccggtcgaga ccaaggaaga cgagcgcgtc
     2401 tgaggagctt gcgatgagcc aagaaccccg ctatctgcag cgctacgacg acagtggccg
     2461 catgctgcat tggagcgtgg cgctgttgtt cttctgcgcc gcgctgtccg gcctggcctt
     2521 gttccatccg gcgctctatt tcttcagtgc cctgttcggc ggcgggccct ggacgcgcat
     2581 cctgcacccc tatatcggcg tgctgatggc gctgagcttc gcggtgatcg cggcccgctt
     2641 gtggcggctc aatctcatcg agcagaacga tcgcgactgg ctagcccatg tcggcgacgt
     2701 gctcaagggc gacaaggaca agaccccgcc cgtggggcgc tacaacggcg gccagaaggt
     2761 cctcttttgg ctgatggtgg cctgccttgc ggtgctgttc gtgaccggct tcgtcttctg
     2821 gcggccctgg ttcgcgccct attttccgat cacgctgatc cgcatcgccg tgctgctgca
     2881 cgcgatggcc gcgacgctgc tgatcctggg catcatcgtc cacatctacg cggcgatttg
     2941 ggtcaagggc acgctgcgcg cgatgacgcg cggcagcgtc agcgaggcct gggccaagca
     3001 gaaccacccg ttgtggcatc gcgagatgac gcagggcaag taggc
//
LOCUS       DASUCY010000218         7014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_100884, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000218 DASUCY010000000
VERSION     DASUCY010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 7014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7014
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100884"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="VFV25_04185"
     CDS             <1..654
                     /locus_tag="VFV25_04185"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY1392545.1"
                     /translation="QATDAARASLKMLLVDDHELVRFGVKALYGELQGVGIDWLEACS
                     LQEAIDIYAREGDIEAVLLDLNLADCKGLQGLRLFLQAHPKARIAVFSGTQDEFVVRQ
                     ARALGAVGYVPKGAMVSEMRASLSALLWPQGGPEERGRASPSALFPRLPVSAMYDRVA
                     ELGPRHLEILELVLSGCTNQEISNAMGLSLGTVKNYVSSLLLALDVKSRSHMISLFR"
     gene            661..2499
                     /locus_tag="VFV25_04190"
     CDS             661..2499
                     /locus_tag="VFV25_04190"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEY1392546.1"
                     /translation="MSTETWGGPETRPEHEGRSSFVAVEGAADSDADSEAAPRRPERR
                     AVDRRLRDAALPAAARASPVVLVAVLAGLLAGINACRPAWPGWSALWAVFGLASWGAW
                     SIVALYLPQFGAGGAKRAWLRPAFPWLGGVCLLSLAFMPWLPGLSESQRWIMLACLGV
                     VALAGAAQLAALPVLAGAAVALPLSVAIAAPVAPVAQAQATAALAICALAALLAQSRC
                     RSWIRVARALIEREDRLRVLETERDTALRADQEKSRFLAIASHDLRQPVHALGLFAAT
                     LERRLQGTAEEPLVRNVMQAIDGLDRSFNAMLDISRLDAGTVAPNIQRFPLRDLFRRL
                     HMHFAGQAELAGLSLRFSPGGKWVSSDPQLLERILGNLIQNAIKYTPRGGIVVVARST
                     RAHTNVEVWDTGVGMRAAELPRIFDEFYQVGHGERRRSQGLGMGLAIVKRLARLLGHG
                     LTVSSQPGRGTMFRLSIAVGGLAGIQDATAAADTLPMPVLQARTVLIIDDEEAICAGL
                     SLLLEEWGYQTASAGSLEQAQHTVRGLAAPPDLILSDLHLGGGPDGIAAIEALRRQFG
                     YDVPAILITGDTSHQEMRRATDSGHPVLFKPVQARKLLNALRGLVS"
     gene            2624..3634
                     /gene="tssA"
                     /locus_tag="VFV25_04195"
     CDS             2624..3634
                     /gene="tssA"
                     /locus_tag="VFV25_04195"
                     /inference="COORDINATES: protein motif:HMM:TIGR03363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HEY1392547.1"
                     /translation="MPDLDLESLLTPLPGDAPCGADLEYDPAFLALEEAGSGRAEQQY
                     GDTVIAAREADWPAVHEQALQLAARTRDLRVAVWLVRGGARVSGLSGAVQGLQLLRGL
                     LERYWDQVHPQLDASDNNDPTARLSALTPLVHADAVLADLRAAGLSAARGGLRVRDIE
                     LALGKAEALPGEAVPTEDGVMQAVAAALAQSAGLGATMQAGLDAARGIAAAVEQRLGA
                     GQGPDFAPLKKLLQPVAEAARRAGAAPDGSTGEPAHAPLAAAAAVGAAGAIASREDAV
                     RALQRVCEWIERNEPSNPAPLLIHRAQRLMSKNFIDIIRDLVPDGLSQVEKLAGIGGE
                     QG"
     gene            3654..4160
                     /gene="tssB"
                     /locus_tag="VFV25_04200"
     CDS             3654..4160
                     /gene="tssB"
                     /locus_tag="VFV25_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HEY1392548.1"
                     /translation="MATSSQKFIARNRAPRVQIEYDVELYGAEKKIQLPFVMGVLSDL
                     SGKPSEPLPPVAERKFLDVDVDNFDARMKAMKPRVAFQVPNTLTGEGNIAVDITFESM
                     DDFSPAAVARKVEGLRQLLEARQQLSNLITYMDGKTGAEELIAKVVKDEALLKSLAAA
                     AKPAESAQ"
     gene            4192..5688
                     /gene="tssC"
                     /locus_tag="VFV25_04205"
     CDS             4192..5688
                     /gene="tssC"
                     /locus_tag="VFV25_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HEY1392549.1"
                     /translation="MAEETVTQPSALQGIEYQGSEFAALLNKEFKPKSDEAKSAVEQA
                     VQTLAQQALAQTKLIGTDVIMSIEALIAELDHKLSQQINAILHHADFQQIESAWRGLH
                     HLVNNTETDEMLKIRVMNISKGDLGKTLKRFKGTAWDQSPLFKKVYEEEFGQFGGEPF
                     GCIMGDYYFDHSPPDTELLGEMSKVAAAAHTPFIAAASPTLMQMGSWQELANPRDLTK
                     IFTTPEYATWRSLRESEDSRYIGLAMPRFLARLPYGAKTSPVEEFNFEEETAGGDHSA
                     YTWANSAYAMAVNINRSFKLYGWCTRIRGIESGGSVEGLPTHTFPTDEGGVAMKCPTE
                     IAISDRREAELAKNGFMPLLHRKNSDFAAFIGAQSLQKPQEYDDPDATANANLAARLP
                     YLFATCRFAHYLKCIARDKVGSFKERDDMAKWLNKWIMNYVDGDPANSSETTKAQKPL
                     AAAEVTVEEIPGNPGYYSSNFFLRPHYQLEGLTVSLRLVSKLPSLKAA"
     gene            5761..6243
                     /locus_tag="VFV25_04210"
     CDS             5761..6243
                     /locus_tag="VFV25_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014251940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HEY1392550.1"
                     /translation="MATIDTHLKLSGVDGESAHKDHKGEIDLLAWSWGVSQASSATAG
                     GGSGKGKATPGDFVFTHFYDKASPLLGKNCANGKHFDQAVVTCRKAGEGQQDFLKVTL
                     KQVLVTSVQPGGSAGGDITENVCLSYADIEFEYKPQDDKGALGGAVKFGWNVASTETR
                     "
     gene            6305..6832
                     /locus_tag="VFV25_04215"
     CDS             6305..6832
                     /locus_tag="VFV25_04215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HEY1392551.1"
                     /translation="MPVSIKEVTAGAGSALPADVDIYLDLTLKRAGKVKGESVSSGHK
                     DDIVVQAFSWGVGAPTDIVTGQQKGRRSYRALSIRKGLDSASSALMSAVATNDEVKSA
                     KLCLRKAGGEQQDYFTMTLEKARVVSVDLESDDRGHPVEKVSFNFQKISVEYRVQGAG
                     GQLGAAHSFNDDLVA"
     gene            6848..>7014
                     /locus_tag="VFV25_04220"
     CDS             6848..>7014
                     /locus_tag="VFV25_04220"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY1392552.1"
                     /translation="MPGFEELIAQGHPREALAELQAQVRARPGEVKLRILLFQLLAVL
                     GQWQRAAQQLDT"
BASE COUNT         1188 a         2400 c         2391 g         1035 t
ORIGIN      
        1 caggcaaccg atgctgcgcg tgcatcgcta aagatgctgc tggtcgacga ccatgagctg
       61 gtgcgcttcg gcgtcaaggc gctgtacggc gaactgcagg gcgtgggtat cgattggctc
      121 gaagcgtgtt cactgcagga agcgatcgac atctatgcgc gcgaaggcga tatcgaggcg
      181 gtgctgctgg acctgaatct ggccgactgc aagggccttc aggggctgcg cctcttcttg
      241 caagcccatc ccaaggcgcg catcgccgtg ttcagcggca cgcaggacga gttcgtggtg
      301 cgccaggcgc gcgcccttgg cgcggtgggc tatgtgccca agggtgcgat ggtgtcggag
      361 atgcgggcca gcctgtcggc cttgctgtgg ccccagggcg gccccgagga aagaggccgg
      421 gcctcgccgt cggcgctgtt cccgcgcctg ccggtgtcgg ccatgtacga ccgcgtggcc
      481 gagctgggtc ccaggcacct cgagatcctc gagctcgtgc tgtcgggctg caccaaccag
      541 gaaatcagca acgcaatggg actgtcgctg ggcacggtca agaactacgt gtcgtcgctg
      601 ctgctggcgc tggatgtgaa gtcgcgatcg cacatgatca gcctgttccg ctgaggagca
      661 ttgagcaccg aaacctgggg tgggcccgag acgcggccgg agcatgaggg ccgctccagc
      721 ttcgttgccg tcgagggggc cgccgacagc gatgccgaca gcgaagccgc accgcggcgc
      781 cccgagcggc gcgccgtcga ccggcgtctg cgcgacgccg ccttgcctgc ggctgcgagg
      841 gcctcgccgg tcgtgctggt cgccgtgctg gctggcctgc tggccggcat caacgcctgc
      901 cgccccgcat ggccgggctg gagcgcgctc tgggccgttt tcgggctggc gtcctggggc
      961 gcctggagca tcgtggcgct gtatctgccg cagttcggcg ccggcggcgc aaaaagggcg
     1021 tggttgcggc cggctttccc ttggctgggc ggggtctgtc tgctgagcct ggcattcatg
     1081 ccctggctgc ccggcctgag cgaatcgcaa cgctggatca tgctcgcttg tctgggcgtc
     1141 gtcgccttgg ccggcgcggc gcagctggcc gcgctgcctg tgctggcggg ggcggcggtt
     1201 gcgctgccgc tctcggtcgc catcgccgca cccgtcgccc cggtggccca ggcgcaggca
     1261 acggctgcgc tggccatttg cgcgctcgct gccttgctgg cgcaatcgcg ctgccgttcc
     1321 tggatcaggg tcgcgcgtgc cttgatcgag cgcgaagacc ggttgcgcgt cctggagacc
     1381 gagcgcgaca cggcgttgcg ggccgaccag gaaaagagcc gcttcctcgc catcgccagc
     1441 cacgacctgc gccagccggt gcatgccctg ggcctgttcg cggccaccct ggagaggcgc
     1501 ctgcagggga ctgccgagga acccctggtg cgcaacgtga tgcaggccat cgacgggctg
     1561 gaccgttcct tcaatgccat gctcgacatc tctcggctgg atgccgggac cgtggcgccc
     1621 aacatccagc gctttccgct gcgcgacctg ttccgccgcc tgcacatgca tttcgcgggg
     1681 caggccgagc tggccgggct gagcctgcgc ttctcgcccg gcggcaaatg ggtcagcagc
     1741 gatccgcagc tgctcgagcg catcctcggc aacctgatcc agaacgccat caagtacacg
     1801 ccgcgcggcg gcatcgtcgt ggtcgcgcgc tcgacgcgcg cgcacaccaa tgtcgaggtc
     1861 tgggataccg gcgtgggcat gcgcgccgcg gagctgccgc gcatcttcga cgagttctac
     1921 caggtcggcc acggcgagcg ccggcgctcc cagggcttgg gcatggggct ggccatcgtc
     1981 aagcggctgg cgcgcctgct cgggcatggg ctcacggtga gctcccagcc ggggcgcggc
     2041 accatgttca ggctcagcat cgcggtgggc gggctggccg ggatccagga cgcgaccgcc
     2101 gccgccgaca cgctgcccat gccggtgctg caggcgcgca ccgtgctcat catcgacgac
     2161 gaagaggcga tctgcgctgg cctgagcctg ctgctggagg aatggggcta ccagaccgcc
     2221 agcgccggct cgctggaaca ggcgcaacac accgtgcgcg gcctggctgc gccgcccgat
     2281 ctgatcctct cggacctgca cctgggcggg ggcccggacg gcatcgcggc gatcgaggcc
     2341 ctgcgccggc agttcggcta cgacgtgccg gccattctga tcaccggcga cacctcgcac
     2401 caggaaatgc gccgcgccac agacagcggg catccggtgc tgttcaagcc cgtgcaggca
     2461 cgcaagctcc tgaacgcgct gcgcggcctc gtctcctgag cgccctgtcg gcttcttgaa
     2521 agtcctcccg gtgttcgccg ccggctaagt gactttcgac cactgtgcgc ctgcgggcac
     2581 acatctagag tgcttgcacg caagcccaag ctcggacagc cgcatgccgg atctcgatct
     2641 cgagtcgcta ctgacgcccc tgcccggcga cgccccctgc ggcgccgacc tggagtacga
     2701 cccggcgttc ctggctctgg aggaggccgg cagcggccgt gcggagcagc agtacggcga
     2761 caccgtcatc gcggcccgcg aggccgactg gcctgccgtg cacgagcagg ccctgcagct
     2821 ggccgcgcgt acgcgcgatc ttcgcgtggc agtctggctc gtgcgcggcg gcgcccgggt
     2881 gtcgggcctg agcggcgcgg tgcaggggct gcagttgctg cgcggcctgc tggagcggta
     2941 ttgggaccag gtccatccgc agctcgacgc cagcgacaac aacgacccca cggcccggct
     3001 gagcgcgctc acgccgctgg tgcatgccga cgccgtgctg gccgacctgc gcgccgcggg
     3061 cctgagcgct gcgcgcggcg gcctgcgcgt gcgcgacatc gagctggccc tcggcaaggc
     3121 cgaggccctg cccggcgagg cggtgcccac cgaagacggc gtgatgcagg ccgtggccgc
     3181 cgcgctggct cagtcggccg ggctgggtgc cacgatgcag gcggggctgg atgcggcgcg
     3241 aggcattgca gccgccgtcg aacagcgcct cggcgcaggg cagggcccgg atttcgcgcc
     3301 gctgaagaag ctcctgcagc ccgttgccga agcggcccgc agagccggcg cggcaccgga
     3361 tggcagcact ggcgagccgg cgcacgcgcc gctggcggct gcggccgctg tcggcgctgc
     3421 gggcgcgatc gcctcgcgcg aggacgccgt gcgcgcgctg cagcgcgtgt gtgaatggat
     3481 agagcgcaac gaaccgagca accccgcgcc gctgctcatc caccgggccc agcgcctgat
     3541 gagcaagaac ttcatcgaca tcatccgcga cctcgtgccc gacgggctca gccaggtcga
     3601 gaagctcgcc ggcatcggcg gcgagcaggg ctagcgcaac aaggagcaaa gccatggcaa
     3661 ccagcagcca gaaattcatc gctcgcaatc gcgccccgcg cgtacagatc gagtacgacg
     3721 tggagctcta cggcgccgag aagaagatac agctgccctt cgtgatgggc gtgctgtcgg
     3781 atctgtccgg caagcccagc gagccgctgc cgccggtggc cgagcgcaag ttcctcgacg
     3841 tcgacgtgga caacttcgac gcccgcatga aggcgatgaa gccgcgcgtg gccttccagg
     3901 tgcccaacac gctgaccggc gaaggcaaca tcgccgtgga catcaccttc gagagcatgg
     3961 acgacttctc gcccgccgcc gtggcgcgca aggtcgaagg cctgcgccag ctgctcgagg
     4021 cgcggcagca actgtccaac ctcatcacct acatggacgg taagaccggg gccgaggagc
     4081 tgatcgccaa ggtggtcaag gacgaggcgc tgctcaagtc gctggcggct gcagccaagc
     4141 ccgccgaatc agcccagtga ggcttacccc agggcatagc ggagatccat catggccgaa
     4201 gaaaccgtca cccagccgtc tgcgctgcaa ggcatcgaat accagggcag cgagttcgcc
     4261 gccttgctga acaaggagtt caagcccaag agcgacgagg ccaagtcggc cgtcgagcag
     4321 gcggtgcaga cgctggcgca gcaggcgctg gcgcagacca agctgatcgg caccgacgtc
     4381 atcatgtcga tcgaggcgct gatcgccgag ctcgaccaca agctctcgca gcagatcaac
     4441 gccatcctgc accacgccga tttccagcag atcgaaagcg cctggcgcgg cctgcaccac
     4501 ctcgtcaaca acaccgagac cgacgagatg ctgaagatcc gcgtgatgaa catctccaaa
     4561 ggcgacctgg gcaagaccct caagcgcttc aagggcacgg cctgggacca gagcccgctg
     4621 ttcaagaagg tctacgagga ggaattcggg cagttcggcg gcgagccctt cggctgcatc
     4681 atgggcgact actacttcga ccacagcccg cccgacaccg agctcctggg cgagatgagc
     4741 aaggtggccg ccgccgcgca cacgcccttc atcgcagcgg ccagcccgac gctgatgcag
     4801 atgggctcct ggcaggaact ggccaacccg cgcgacctga ccaagatctt caccacgccc
     4861 gaatacgcca cctggcgctc gctgcgcgaa tccgaggact cgcgctacat cggcctggcg
     4921 atgccgcgct tcctggcccg cctgccctat ggcgccaaga ccagcccggt ggaggagttc
     4981 aacttcgagg aagaaaccgc aggcggcgac catagcgcct atacctgggc gaactccgcc
     5041 tatgcgatgg cggtcaacat caaccgctcg ttcaagctgt atggctggtg cacgcgcatc
     5101 cgcggcatcg aatcgggcgg ctcggtggag ggcctgccca cgcatacctt cccgaccgac
     5161 gaaggcggcg tggcgatgaa atgccccacc gagatcgcga tcagcgaccg gcgcgaggcc
     5221 gagctggcca agaacggctt catgccgctt ttgcaccgca agaactcgga cttcgcggcc
     5281 ttcatcggcg cccagtcgct gcagaagccg caggaatacg acgaccccga cgctaccgcc
     5341 aacgcgaacc tggcggcccg gctgccctat ctgttcgcca cctgccgctt cgcgcactac
     5401 ctcaagtgca tcgcgcgcga caaggtcggc tccttcaagg agcgcgacga catggccaag
     5461 tggctcaaca aatggatcat gaactacgtg gacggcgacc cggccaattc ctcggaaacc
     5521 accaaggcgc agaagccgct ggccgccgcc gaggtgacgg tggaagagat tcccggcaac
     5581 ccgggctact acagctccaa tttcttcctg cggccgcact accagctcga aggcctcacc
     5641 gtttcgctgc ggctggtctc caagctgccg tcgctgaagg cggcgtagcg agcctgcgcc
     5701 tagcaaaccc atcccccaac cgcgcgtcac ccacgcatcg tttcctcaag gagttgcacc
     5761 atggccacca tcgacacaca tctgaaactg agcggcgttg acggcgagtc ggcccacaag
     5821 gaccacaagg gcgagatcga cctgctggcc tggagctggg gcgtctctca ggcgtccagc
     5881 gccacggccg gcggcggctc cggcaagggc aaggccacgc ccggagactt cgtcttcacg
     5941 catttctacg acaaggcctc gcccctgctc ggtaagaact gcgccaacgg caagcatttc
     6001 gaccaggccg tggtgacctg ccgcaaggcc ggcgaaggcc agcaggactt cctcaaggtc
     6061 acgctgaagc aggtgctcgt cacctcggtg cagcccggcg ggtccgcggg cggcgacatc
     6121 accgagaacg tgtgcctgag ctatgccgac atcgagttcg aatacaagcc gcaggacgac
     6181 aagggcgcgc tgggcggcgc cgtgaagttc ggctggaacg tggcgtccac cgagacgcgt
     6241 tgacgcccaa gggcaaggcg gccgtcgcct ctgcggcgcc gcgccgccat gccgggagtg
     6301 agcgatgccg gtatcgatca aggaagtgac cgctggagcc ggcagcgcct tgccggccga
     6361 cgtcgacatc tatctcgacc tcacgctcaa gcgcgcgggc aaggtcaagg gcgaatcggt
     6421 ctcctccggg cacaaggacg acatcgtcgt gcaggctttc tcctggggcg tgggcgcccc
     6481 gaccgacatc gtgaccggcc agcagaaggg gcggcgcagc taccgggcct tgagcatacg
     6541 caaggggctg gacagcgcca gcagcgcgct gatgagcgcg gtggcgacca acgacgaggt
     6601 caagagcgcc aagctctgcc tgcgcaaggc gggcggcgaa cagcaggact acttcacgat
     6661 gacgctggag aaggcgcgtg tggtcagcgt cgatctggaa agcgatgacc gcggccaccc
     6721 ggtcgagaag gtgagcttca acttccagaa gatctcggtc gaatatcgcg tgcagggcgc
     6781 cggcggccag ctcggagccg cccacagctt caacgacgac ctggtcgcct gagcggggtt
     6841 tgcagccatg cctggtttcg aagagctgat cgcgcaaggc cacccccggg aggccctggc
     6901 ggagctgcag gcgcaggtca gggcgcggcc tggcgaagtg aagctgagga tcctcttgtt
     6961 ccagctgctg gccgtgctgg ggcaatggca acgagcggcc caacaactcg atac
//
LOCUS       DASUCY010000219         2180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_52690, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000219 DASUCY010000000
VERSION     DASUCY010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52690"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..947
                     /locus_tag="VFV25_04225"
     CDS             87..947
                     /locus_tag="VFV25_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6544 family protein"
                     /protein_id="HEY1392553.1"
                     /translation="MGWLMLVVRIAGVLVLALGLLLAFGAWRWRDATSALMQRLDAAA
                     TPQPGQRYSERQLDGLPAPVQRYFRAVLQEGAPVVASATLAQQGSLNMGEAGDRWLAF
                     TSRQRVVTRRPGFVWDARVMLAPGLAIRVQDAYVAGEGQLCAALLGLFTLAKLRGKDE
                     MARGELMRYLAEAAWYPTALLPGQGVSWQAIDDTSARATLSDGEAAASLVFRFGADSL
                     IETVYADARGRTVAGQIVPTPWEGRWADYRLQAGMLVPTRGEVSWLLPQGRKPYWRGV
                     LTSVSYGLAA"
     gene            complement(1041..>2180)
                     /locus_tag="VFV25_04230"
     CDS             complement(1041..>2180)
                     /locus_tag="VFV25_04230"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY1392554.1"
                     /translation="KTAGGCVAVWPHDYMKNGTNTIFEVIHEAGLRTAWSDKHPAYEI
                     LNGPSGRGLDELYAPEINSTALQTNGFPSAPADADWTTDPTYTRAYDGFKVQAVLNWI
                     QGLDHSGSKQVGTPAIFGMNFQAVSVAEKVTKAMEGATITRGGYLDAAATPTLPLQQS
                     LDFVDASLGRMVDALAARHLLHSTLIIVGAKHAQSPIDLKTLHMLSGSKNAYATADVT
                     DPATLLANGGVPVAQETADDVSLLWLASHGDVDKAVAILSADQATINSTKIQTLYHGP
                     ALKSQWGDPAQGRVPDLIIQPIPGTIYSGSAKKLAEHGGFAHDDTNALLVVSNPRLDA
                     RVIDSPVTNMQVAPTILKVLGLKPHALQAVRREGTHVLPGLELED"
BASE COUNT          300 a          716 c          778 g          386 t
ORIGIN      
        1 atcgacagct tcactcttcc cttgactcca ctcaaagccc cggtgcgcca aacgccccaa
       61 gatgccggtg tgcaaagggg atggcgatgg gatggctgat gctcgtggtg cggatcgccg
      121 gcgtgcttgt gctggccttg ggcttgctgc tggccttcgg tgcgtggcgc tggcgtgacg
      181 ccacttccgc cttgatgcag cggctcgatg cagcggccac gccccagcca ggccagcgct
      241 acagcgagcg acagctcgac gggctgccgg ccccggtgca gcgctacttc cgcgccgtct
      301 tgcaggaagg tgcacccgtc gtggcttcgg ccacgctggc ccagcaaggc agcctcaaca
      361 tgggcgaagc cggcgatcga tggcttgctt tcacctcgcg ccagcgcgtc gtgactcgcc
      421 ggcccggctt cgtctgggac gcccgcgtca tgctggcgcc cgggctggcc atccgcgtgc
      481 aagacgcata cgtggcgggc gagggccagc tatgcgccgc gctgctgggt ttgttcaccc
      541 tggccaagct gcgcggcaag gatgaaatgg cgcgcggcga gttgatgcgc tacctcgccg
      601 aggcggcttg gtacccgacc gccctcctcc caggccaagg agtgagctgg caggccatcg
      661 acgacacatc ggcccgcgcc acgctgagcg atggcgaagc cgcggccagc ctggtctttc
      721 gattcggggc cgacagcttg atcgaaaccg tttatgccga tgcgcgcggg cgcaccgtgg
      781 cagggcagat cgtgccgaca ccctgggaag gccgctgggc cgattaccgg ctgcaagccg
      841 gcatgctggt gccgacgcgg ggcgaggtgt cgtggttgct gccgcagggc cgcaagccct
      901 actggcgcgg cgtgctcacc tcggtgtcgt acggcttggc ggcctgacac cggcaccgcc
      961 tgtcgagttc ttccggtccc gcgggtgcgg gagcccggtc gaccatcgac gaggcggtgc
     1021 agggccgcct ggccgccgga tcagtcttcc agttccaggc ccggcagcac gtgcgtgcct
     1081 tcacggcgca cagcttgcag ggcatggggc ttcaggccca gcactttcag gatcgtcggg
     1141 gcgacctgca tattggtcac cggcgagtcg atcacgcgcg catccaggcg cggattcgac
     1201 acgaccagca gcgcgttcgt gtcgtcatgc gcgaatccgc cgtgctcggc cagcttcttg
     1261 gcgctgccgc tgtagatcgt gcccgggatc ggctggatga tgaggtcggg cacccggccc
     1321 tgggcggggt cgccccactg gctcttgagc gccgggccgt gatacagggt ctggatcttg
     1381 gtggagttga tcgtggcctg gtcggcgctc aggatggcga ctgccttgtc cacgtcgccg
     1441 tggctggcga gccacagcag cgacacgtcg tctgcggtct cctgggccac cggcacgccg
     1501 ccgttggcca gcagcgtcgc cgggtcggtg acgtcggccg tggcgtatgc attcttcgag
     1561 ccgctgagca tgtgcagcgt cttgaggtcg atcggcgact gggcatgctt ggcgccgacg
     1621 atgatcagcg tcgaatgcag caaatgccgc gcggccagcg catcgaccat gcggccgagc
     1681 gaggcgtcga cgaagtccag cgactgctgc agcggcagcg tgggcgtggc cgccgcatcg
     1741 agatagccgc cgcgcgtgat cgtggcgccc tccatcgcct tggtcacctt ctcggcgacg
     1801 ctgaccgcct ggaagttcat gccgaagatc gcgggggtgc cgacctgctt gctgccgcta
     1861 tggtccaggc cctggatcca gttcagcacg gcctggacct tgaagccgtc ataggcgcgc
     1921 gtgtaggtcg ggtccgtggt ccagtcggcg tccgcagggg cgctggggaa gccgttggtc
     1981 tgcagcgcgg tggagttgat ctcgggggcg tagagctcgt ccagcccgcg accggacgga
     2041 ccgttgagga tctcgtaggc cgggtgcttg tcggaccatg cagtgcgcag gccggcctcg
     2101 tggatgacct cgaagatcgt gttggtcccg ttcttcatgt agtcgtgcgg ccagacggcc
     2161 acgcacccgc ccgcggtctt
//
LOCUS       DASUCY010000220         2371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_422135, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000220 DASUCY010000000
VERSION     DASUCY010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422135"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VFV25_04235"
     CDS             complement(<1..219)
                     /locus_tag="VFV25_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEY1392555.1"
                     /translation="MTRQIFLDTETTGLSPETGDRVIEIGCVEMMNRRLTGNNKHFYL
                     NPERANSEDAVKIHGLTDEFLADKPLFAG"
     gene            complement(417..755)
                     /gene="fdx"
                     /locus_tag="VFV25_04240"
     CDS             complement(417..755)
                     /gene="fdx"
                     /locus_tag="VFV25_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015421518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HEY1392556.1"
                     /translation="MTTIKILPHQEYCPEGKTIEAEPGSSICEALLEQGIPIEHACEM
                     SCACTTCHVIVREGFNSLGEMDESEEDLLDRAWGLEPNSRLSCQAIVAQKDLVIEIPK
                     YSINHAKEEH"
     gene            complement(773..>2371)
                     /gene="hscA"
                     /locus_tag="VFV25_04245"
     CDS             complement(773..>2371)
                     /gene="hscA"
                     /locus_tag="VFV25_04245"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HEY1392557.1"
                     /translation="KRFMGRGLADIADRARLPYHFVDAPGMLSLQTREGVKSPVEVSA
                     EILAALRQRAEDSFADEIFGAVITVPAYFDEAQRQATKDAARLAGLNVLRLINEPTAA
                     AIAYGLENSAEGLYAVYDLGGGTFDVSLLRLTRGVFEVVATGGDSALGGDDYDHAIAE
                     WAERKTGISARTAQDKRTLLVAARAAKEALTEAPQAKFAVQMDGGSIALELTRADFDE
                     LTRALTERTLATVRKVLRDARVTKDEVHGIVLVGGATRMPQIREAVGQHFGRAPLVNL
                     NPDEVVALGAAIQANQLAGNSREGELLLLDVIPLSLGLETMGGLVERIIPRNSTIPTA
                     RAQDFTTFKDGQTALVIHVVQGEREQVEHCRSLARFELRGIPPMVAGAARIRVSFQVD
                     ADGLLSVSAREQTTGVEAAIAVKPSYGLSDDQIAAMLVEGFKAADEDMRDRALRESRV
                     EAERMVLATLSALAADGDLLSPAERTAVDALIADVQDQAKGEDHHAIDAVVEALAKGT
                     EAFAAERMNRGIRQALAGRSVEEV"
BASE COUNT          332 a          799 c          804 g          436 t
ORIGIN      
        1 gccggcgaac aagggcttgt cggccaggaa ctcgtcggtg aggccgtgga tcttgacggc
       61 gtcctcgctg ttggcccgct cggggttgag atagaagtgc ttgttgttgc cggtcagccg
      121 ccggttcatc atctcgacgc agccgatttc gatgacgcgg tcgcccgttt ctgggctcag
      181 gccggtggtt tcggtgtcga ggaagatctg acgggtcacg acagcccccc gctcgccgag
      241 tacgtcgagc acccctccga gaggggggcg ggccttgctc ggggtgggtg ccgcggctcc
      301 aagccgacct gcgtcggctt ggacggcacg aacgcccgcc gactctggcg acaggctgtt
      361 cggcccggcg cggcgacccg cggggcatct tcaatccaca tcgagcttct catccctcag
      421 tgttcttcct tggcgtggtt gatcgagtac ttcgggatct cgatcaccag atccttctgc
      481 gccacgatgg cctggcagct caagcgcgag ttgggctcca gcccccaggc gcggtcgagc
      541 agatcttcct cgctctcgtc catctcgccc aatgagttga aaccctcgcg cacgatgacg
      601 tggcaggtcg tgcaggcgca gctcatttcg caggcatgct cgataggaat accctgctcg
      661 agcagggctt cgcagatcga gctgcccggt tcggcctcga tggtcttgcc ctcggggcag
      721 tactcttgat gcggcaggat cttgatcgtt gtcatcggga cgctgtacct cgctatacct
      781 cttcgacgct gcggccggca agcgcctggc gtatgccgcg gttcatgcgt tcggcggcga
      841 aggcctcggt gcctttggcc aaggcctcca cgaccgcgtc tatggcgtgg tgatcctcgc
      901 ccttggcctg gtcctgtaca tcggcgatca aggcgtcgac ggcggtgcgc tcggcggggc
      961 tcagcaggtc gccgtcggct gccagcgccg atagcgtcgc gagcaccatg cgttcggcct
     1021 cgacgcgcga ctcgcgcagg gctcggtcgc gcatgtcctc gtcggccgcc ttgaagcctt
     1081 ccaccagcat cgcggcgatc tggtcgtcgc tgaggccgta gctgggcttg acggcgatcg
     1141 cggcttcgac gcccgtggtc tgctcgcgcg ccgacacgct gagcaggccg tcggcatcga
     1201 cctggaagct cacgcgtatg cgcgccgcgc cggccaccat cggcgggatg ccgcgcagtt
     1261 cgaagcgagc cagcgagcgg caatgctcga cctgctcgcg ctcgccctgc acgacatgga
     1321 tcaccaatgc cgtctgcccg tccttgaagg tcgtgaagtc ctgggcccgc gcggtgggga
     1381 tcgtggaatt gcgcgggatg atgcgctcga ccaggccgcc catggtttcc aggcccagcg
     1441 acagcggaat cacgtccagc aacagcagct cgccctcgcg gctgttgccg gccagctggt
     1501 tggcctggat cgcggcgcct aaggccacca cttcgtccgg gttgagattg acgagcggcg
     1561 cgcggccgaa atgctgcccc actgcttcgc gtatctgcgg catgcgggtc gccccgccga
     1621 ccagcacgat gccgtggacc tcgtcctttg tgactctggc gtcgcgcagc accttgcgca
     1681 cggtggccag cgtgcgctcg gtgagggcgc gcgtcagctc gtcgaagtcc gcacgcgtca
     1741 gctccagcgc gatgctcccg ccgtccatct gcacggcgaa cttggcctgc ggtgcttcgg
     1801 tcagcgcttc cttggcagcg cgcgccgcga ccagcagggt tcgcttgtcc tgggccgtgc
     1861 gggcggagat gccggttttg cgctcggccc actcggcgat cgcatggtcg tagtcgtctc
     1921 cgcccagggc cgagtcgccg ccggtagcga cgacttcgaa aaccccgcgt gtcaggcgca
     1981 gaagcgagac gtcgaaggtg ccgccgccca ggtcgtagac cgcgtacagg ccctcggcgc
     2041 tgttctccag gccataggcg atcgccgccg ccgtaggctc gttgatgagg cgcagcacat
     2101 tgaggccggc cagcctggcg gcatccttgg tggcctggcg ctgggcctcg tcgaaatagg
     2161 cgggcacagt gattacggca ccgaagatct cgtcagcgaa gctgtcttca gcgcgctggc
     2221 gcagggccgc caggatctcg gccgacacct cgaccggcga cttgacgcct tcgcgcgtct
     2281 gcagactcag catgccgggc gcatcgacga agtggtaggg cagccgggcg cggtcggcga
     2341 tgtcggccag gccgcgcccc atgaagcgct t
//
LOCUS       DASUCY010000221         3311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_984455, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000221 DASUCY010000000
VERSION     DASUCY010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_984455"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VFV25_04250"
     CDS             <1..195
                     /locus_tag="VFV25_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HEY1392558.1"
                     /translation="TAPQAAGVIHTDFERGFIRAQTIAYDDFIAYKGEQGAKDAGKMR
                     AEGKEYVVKDGDVMNFLFNV"
     gene            300..>3311
                     /locus_tag="VFV25_04255"
     CDS             300..>3311
                     /locus_tag="VFV25_04255"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HEY1392559.1"
                     /translation="MSGNTALTASLRFLLTAAAYALVGMAALLLAIPTGHASPLFPSA
                     GIALVCVLFYGPRLTAAVALGSFALNLWHSHQQGITGAAGYLLPACIALGAAAQAWVG
                     AFLVRRYVAPPFTLDAPREIGRFLLLGGALACLVSGSVAVSALALFGAVPREQLAYTW
                     WIWWSGDTLGVLIAAPIVLCLIGQPRSDWAARRLTVALPLAVATLLLSLAIIEVSRWD
                     EQRVQVNFERDAVNLSNAVQLRMQAHLDALDAMYGVYAASEEAEITREEFRRAATPWL
                     HKLPGLQAIGFHQRVARSDIPAFEMQVRKEGLADFHVFDRPEHPVPGDAEVMAMRYIE
                     PLAGNASALGVNVFSIPASRAAIGLSQRSDQAIASAAFRLTQDTPDRAGKAGSEQAGV
                     VVYRAVYKGSPRTPEERIAATRGTAFITLRMGDAMHAVAGDMPAYLGLCLLDADPAAP
                     RRRLVGPEGCEQASASRATTLRHVTALAFAGRQWELRVTAPHNALASSQQGSAGLFSG
                     IGLLATTLLGALLLVVTGRTQRIEVAVRERTTQLEEEIHERRIIDAALRESEQRFRSI
                     FNTVPIGVVYTDLDWRVRQPNAAFCRLTGYSPEELSTMTLEQLTHPEDREADRDAYRQ
                     LIAGEMPTYRRNRRYLARNGRVLRVNVTIALLHDERGQPRRAVGVVEDISEHLRLLEA
                     EQRREAAEASNRAKSEFLSRMSHELRTPLNAMLGFAQLMSLDDQRPLSERQAQWVAQI
                     QHAGWHLLEMINDVLDLSRIESGTVSLQVESLDLAELVRASAVLIEAEAAKRGISLLL
                     DLAPDARAVLGDATRVKQILTNLLSNAVKYNRDGGSIRLRARPDRSREGVEIEISDTG
                     IGMNETQLASLFQPFNRLGRERSGPDGTGIGLVIARRLAELMGGSLRASSVENQGSSF
                     TLTLPQAHAPDAVPSELDRLGLLTDDTTYHRRRVHYIEDNETNAEVMRGMLLQRPQVA
                     LEISSTGLDGLAAIKQHRPDLVLLDMHLPDID"
BASE COUNT          520 a         1175 c         1103 g          513 t
ORIGIN      
        1 accgcgccgc aggcagccgg cgtgatccat accgatttcg agcgcggctt catccgcgcg
       61 cagaccatcg cctacgacga cttcatcgcc tacaagggcg agcagggtgc caaggacgcc
      121 ggcaagatgc gcgcggaagg caaggaatac gtcgtcaagg acggcgacgt gatgaatttc
      181 ctattcaatg tctgacctgt agcactttcc agcttgtcag ggggctgccc gaggggatac
      241 tgccgccgtc atctccgccc gggcgccgcg gcttgttgcg gccctaatcc atgcccgcta
      301 tgtccggcaa cacggccctg actgcgtccc tgcgattcct gctcaccgcc gcggcctatg
      361 cgctcgtcgg catggcggcg ctgctgctgg ccatccccac gggacatgcc tcgcctttgt
      421 ttccttcggc cggcatcgcg ctagtttgcg tgctgtttta tggcccgcgc cttaccgccg
      481 cggtggcact gggctccttc gcgctcaatc tctggcacag ccaccagcag gggatcacag
      541 gggccgccgg ctacctcctg ccggcctgca tcgccctggg cgcggcagcg caggcctggg
      601 tgggcgcgtt cctggtgcgc cgctatgtag cgccgccttt tacgctggat gcgcctcgcg
      661 agatcgggcg cttcctgctg ctgggcgggg ccttggcctg cctggtcagc ggcagcgtag
      721 ccgtctcggc gctggccctg ttcggcgcgg tgccgcgcga gcagctggcc tacacctggt
      781 ggatctggtg gagcggcgac acgctgggcg tgctgatcgc cgcccccatc gtgctgtgcc
      841 tgatcggcca gccgcgcagc gactgggcgg cgcgccgcct caccgtggcg ctgccgctgg
      901 cggtcgccac cctgctgctg tcgctggcca tcatcgaggt cagccgctgg gacgagcagc
      961 gcgtgcaggt caatttcgag cgcgacgccg tcaacctctc caatgccgtt caattgcgca
     1021 tgcaggcgca tctggatgcg ttggatgcga tgtacggcgt ctacgccgca tcggaagaag
     1081 ccgagatcac gcgcgaggag tttcgccgcg ctgcgacacc ctggctgcac aagctgcctg
     1141 gcttgcaggc gataggcttt caccagcgtg tcgcgcgctc cgacattccc gccttcgaga
     1201 tgcaggtgcg caaggagggg ctggcggact tccatgtctt cgaccgcccc gagcatccgg
     1261 tccccggcga cgcggaggtg atggcgatgc gctacatcga gcccctcgcc ggcaacgcga
     1321 gcgccctggg cgtcaacgtg ttcagcatcc cggcctcgcg cgcggccatc ggcttgagcc
     1381 agcgcagcga ccaggccatc gccagcgcgg cctttcggct gacgcaggac actcccgacc
     1441 gggctggcaa agccggcagc gagcaggccg gcgtggtggt ctaccgtgcg gtctacaagg
     1501 gcagtcccag gacacccgaa gagcgcatcg cggccacgcg cggcaccgca ttcatcaccc
     1561 tgcgcatggg cgatgcgatg cacgccgtgg ccggcgacat gccggcctac ctcgggctgt
     1621 gcctgctcga cgccgatccc gccgcgccgc gccgccgcct cgtcggcccc gagggctgcg
     1681 agcaggcatc tgcatcgcgc gcaacaacac tgcggcatgt gacggcgctg gccttcgccg
     1741 ggcgccaatg ggagctgcgc gtcacggccc cgcacaacgc cctggccagc agccagcagg
     1801 gcagcgccgg gctgttctcc gggatcggcc tgctggccac gacgctgctg ggcgcgctgc
     1861 tgctcgtcgt gaccgggcgc acgcagcgca tcgaggtggc cgtgcgcgag cgcacgacgc
     1921 agctggagga agaaatccac gagcgccgca tcatcgacgc ggcgctgcgc gagagcgagc
     1981 agcgctttcg cagcatcttc aacaccgtgc ccatcggcgt ggtctacacc gacctggact
     2041 ggcgcgtgcg ccagcccaat gccgccttct gcaggctcac cggctacagc cccgaggagc
     2101 tgagcacgat gacgctggag cagctcaccc accccgagga tcgcgaggcc gatcgcgacg
     2161 cctatcgcca gctgatcgcc ggcgagatgc cgacctaccg ccgcaaccgc cgctacctgg
     2221 cgagaaacgg gcgcgtactt agggtcaatg tgacgatcgc gctgctgcac gacgagcgcg
     2281 gccagccgcg gcgcgccgtc ggcgtggtgg aggacatctc cgagcatctg cgcctgctgg
     2341 aagccgagca gcggcgcgaa gccgccgagg cctccaaccg cgccaagagc gagttcctat
     2401 cgcgcatgag ccatgaactg cgcacgccgt tgaatgccat gctcggtttc gcccagctga
     2461 tgagcctgga cgatcagcgg cccttgtccg agcgccaagc gcaatgggtg gcgcagatcc
     2521 agcatgcggg ctggcatttg ctggagatga tcaacgacgt gctggatctc tcgcgcatcg
     2581 aatccggcac ggtgagcctg caggtcgaat cgctggacct cgccgagctg gtgcgtgcct
     2641 cggccgtgct gatcgaggcc gaggccgcca agcgcggcat cagcctgctg ctggaccttg
     2701 cgcccgacgc ccgagcggtg ctgggcgacg ccacgcgagt caagcagatc ctgaccaacc
     2761 tgctgagcaa tgccgtcaag tacaaccgcg acggcggcag catcaggctg cgcgccaggc
     2821 ccgacaggag ccgcgagggc gtcgagatcg aaatcagcga caccggcatc ggcatgaacg
     2881 aaacccagct cgcctcgctg ttccagccct tcaaccgcct gggccgcgag cgctccggcc
     2941 cggacggtac cggcatcggc ctcgtcatcg cccggcgcct ggccgagctg atgggcggca
     3001 gcctgcgcgc gagcagcgtc gagaaccagg ggtctagctt cacgctgacg ctgccgcagg
     3061 cccatgctcc cgacgcggtg ccgtcggagc tggacaggct gggcctcctc accgacgata
     3121 cgacctatca ccggcggcgc gtgcactaca tcgaggacaa cgagaccaat gccgaagtga
     3181 tgcgcggcat gctgctgcag cgtccgcagg tcgcgctcga aatctcgagc accggcctgg
     3241 acgggctggc cgccatcaag cagcatcgcc ccgacctggt cctgctggac atgcacctgc
     3301 cggacatcga c
//
LOCUS       DASUCY010000222         3314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1032642, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000222 DASUCY010000000
VERSION     DASUCY010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1032642"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..790
                     /locus_tag="VFV25_04260"
     CDS             35..790
                     /locus_tag="VFV25_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEY1392560.1"
                     /translation="MKAMVAVKRVVDYNVKVRVKSDGTGVDIANVKMSMNPFDEIAVE
                     EAVRLKEKGLVSEIIAVSCGVTACQETLRTAMAIGADRAILVETPAELELQPLAVAKL
                     LKALVDKEQPGLVILGKQAIDDDCNQTGQMLAALAKLPQATFASKLEIADGAATVTRE
                     VDGGLETVKLKLPAVVTTDLRLNEPRYVTLPNIMKAKKKPLEVLNPAQLGVDVAPRIK
                     TLKVAEPPKRGAGIKVPDVATLVAKLKNEAKVI"
     gene            791..1738
                     /locus_tag="VFV25_04265"
     CDS             791..1738
                     /locus_tag="VFV25_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEY1392561.1"
                     /translation="MSVLVIAEHDHGALKGATLNTVTAAAACGGDVHVLVAGHNAAGA
                     AQAAAQIAGVAKVLHADGASLAEQLAENVAAQVLTIVGDGAKNYSHVLFPATAHGKNV
                     APRVAALLDVAQISDITKVISADTFERPIYAGNAIATVQSADAIKVVTVRTTNFDAAP
                     AQGGSAAVESIAAVADGGTSSFVGAEIAKLDRPELTAAKIIVSGGRALGSSDKFNEVL
                     TPLADKLGAALGASRAAVDAGYAPNDWQVGQTGKIVAPQLYIAAGISGAIQHLAGMKD
                     SKVIVAINKDPEAPIFSIADYGLEADLFEAVPAMVKELG"
     gene            1830..>3314
                     /locus_tag="VFV25_04270"
     CDS             1830..>3314
                     /locus_tag="VFV25_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY1392562.1"
                     /translation="MSYQAPIKDMLFDMKELAGLEQVAQLPGYEEAGLETAQAVLEEC
                     AKFNEGVVAPLNFEGDKNPSSWKDGKVTTTPGFKEAFRQFAEGGWQGLQHPTEFDGQG
                     LPKTIGAACVEMLNSANLSFALCPLLTDGAIEALLTAGTPEQQQTYIPKMINGAWTGT
                     MNLTEPQAGSDLALVRTRAEPQGDGSYKLFGTKIFITYGEHDMAENIVHLVLARVTGA
                     PEGVKGISLFLCPKFMVKPDGSLGERNDAHCVSIEHKLGIKASPTAVLQFGDHGGAIG
                     YLVGQENRGLEYMFIMMNAARYAVGLQGIAVAERAYQKAAAYAKERVQSRPVDGSLPG
                     AAPIVHHPDVKRMLMTMRALTEACRAMALTAAAAHDAAHAHPDAEQRKQNQACYEFLV
                     PLVKGYSTEMSLEVTSLGVQVHGGMGFIEETGAAQYYRDARILTIYEGTTAIQANDLV
                     GRKTARDGGAVAKAVARQIAATEAALAPRKSVAARAMLQRLKLAR"
BASE COUNT          637 a         1099 c         1120 g          458 t
ORIGIN      
        1 ttcgggcttg attgaaactg caggagaacc tcgcatgaag gcaatggtcg cggtcaaacg
       61 cgtggtggac tacaacgtga aggtgcgcgt gaagtccgac ggcacggggg tggacatcgc
      121 caacgtgaag atgagcatga accccttcga cgagatcgcc gtggaagagg cggtgcgcct
      181 gaaggagaag ggcctcgtca gcgagatcat cgccgtgtcc tgcggcgtga cggcctgcca
      241 ggagaccctg cgcacagcga tggccatcgg cgccgaccgc gccatcctgg ttgagacgcc
      301 tgccgagctc gaactgcagc ccctggccgt ggccaagctg ctcaaggccc tggtcgacaa
      361 ggaacagccc ggcctcgtga tcctgggcaa gcaggccatc gacgacgact gcaaccagac
      421 cggccagatg ctggccgcgc tcgccaagct gccgcaagct actttcgcca gcaagctcga
      481 gatcgccgac ggtgcggcca ccgtcacccg cgaggtcgac ggcggcctgg agaccgtcaa
      541 gctcaagctg ccggccgtgg tgaccaccga cctgcgcctc aacgagccgc gctacgtgac
      601 gctgcccaac atcatgaagg ccaagaagaa gccgctggag gtattgaatc cggcccaact
      661 gggcgtggac gtggccccgc gcatcaagac cctcaaggtg gccgagccgc ccaagcgcgg
      721 cgctggcatc aaggtgcccg acgtcgccac tctcgtggcc aagctgaaga acgaagccaa
      781 ggtgatctga atgagcgtcc tcgtcattgc tgaacacgac cacggcgccc tcaagggcgc
      841 gaccctgaat accgtcactg ccgcagcagc ttgcggcggc gacgtccatg tgctggtcgc
      901 cggccacaac gcagccggcg cggcccaggc ggcggcgcag atcgccggcg tggccaaggt
      961 gctgcatgcc gacggcgcga gcctggccga gcagctggcc gagaacgtcg cggcccaggt
     1021 gctgacgatt gtgggtgatg gggccaagaa ctacagccac gtgctcttcc ccgccaccgc
     1081 gcacggcaag aacgtcgcgc cgcgcgtggc ggcgctgctg gacgtggccc agatcagcga
     1141 catcaccaag gtgattagtg ctgatacctt cgagcgccca atctacgccg gcaacgccat
     1201 cgccacggtg caaagcgccg atgcgatcaa ggtggtcacg gtgcgcacca ccaacttcga
     1261 tgccgcgccg gctcaaggcg gtagcgcggc ggtcgagagc atcgctgcgg tggccgatgg
     1321 cggcacttcc agcttcgtcg gcgccgagat cgccaagctg gatcgccccg agctgacggc
     1381 ggccaagatc atcgtctcgg gcggccgcgc gctcggctcg agcgacaagt tcaacgaagt
     1441 gctgaccccg ctggccgaca agctgggcgc cgctttgggc gccagccgcg cggcagtgga
     1501 cgccggctat gcgcccaacg actggcaggt gggccagacc ggcaagatcg tcgcgccgca
     1561 gctctacatc gcggcaggca tcagcggggc gatccagcac ctcgcgggca tgaaggactc
     1621 caaggtgatt gtggcgatca acaaggatcc cgaggcgccg atcttctcaa tcgccgacta
     1681 cgggctcgag gccgatctgt tcgaggccgt gcccgcgatg gtgaaagagc tcggctgacg
     1741 ggcaggaagc gaccgcgaca caaggcgccg cgactggcaa cagtccaggc gcctttgttt
     1801 tgaagttttg aaaagacaag gagactgcaa tgagctatca agcgcccatc aaggacatgc
     1861 tcttcgacat gaaggagctc gccggccttg agcaagtggc ccagctgccc ggctacgagg
     1921 aagctggcct ggagacggcg caagccgtgc tcgaggaatg cgccaagttc aacgagggcg
     1981 tggtcgcgcc cctgaacttc gagggcgaca agaacccgag ctcctggaag gacggcaagg
     2041 tcacgaccac gccgggcttc aaggaggcct tcaggcagtt cgccgagggc ggctggcagg
     2101 gcctgcagca tccgaccgag ttcgacggcc agggcctgcc caagaccata ggcgcggcct
     2161 gcgtcgagat gctcaacagt gcgaacctga gcttcgcgct gtgcccgctg ctcaccgatg
     2221 gcgcgatcga ggccttgctc accgccggca cgcccgagca gcagcagacc tacatcccca
     2281 agatgatcaa cggggcgtgg accggcacga tgaatctgac cgagccgcag gccggctccg
     2341 atctcgcgct ggtgcgcacc cgcgccgagc cccagggcga cggcagctac aagctcttcg
     2401 gcaccaagat cttcatcacc tatggcgagc acgacatggc cgagaacatc gtccacctcg
     2461 tgctggcccg cgtgaccggg gcacccgagg gcgtgaaggg catctcgctc ttcctctgcc
     2521 cgaagttcat ggtcaaaccg gacggcagcc tgggcgagcg caacgacgcg cactgcgtga
     2581 gcatcgagca caagctgggc atcaaggcca gcccgacggc ggtgctgcag ttcggcgacc
     2641 atggcggcgc catcggttat ctcgtgggcc aggagaaccg cggcctcgaa tacatgttca
     2701 tcatgatgaa cgcggcccgc tatgcagtgg gcctgcaggg catcgcggtg gccgagcgcg
     2761 cctaccagaa ggcggcggcc tatgcgaagg agcgcgtgca gtcgcggccg gtggatggct
     2821 cgctgcccgg cgcggcgccc atcgtgcatc accccgatgt gaagcgcatg ctgatgacca
     2881 tgcgcgcgct gaccgaagcc tgccgcgcga tggcgctcac cgcggcggcg gcccacgacg
     2941 cggcccatgc ccaccccgat gccgagcagc gcaagcagaa ccaggcctgc tacgaattcc
     3001 tggtgccgct ggtcaagggc tacagcaccg agatgagcct ggaagtcacc tcgctgggcg
     3061 tgcaggtgca cggtggcatg ggcttcatcg aggaaaccgg cgcggcccag tactaccgcg
     3121 atgcccgcat cctcacgatc tacgagggca cgacggcgat ccaggccaac gacctggtgg
     3181 gccgcaagac ggcgcgcgac ggcggtgcgg tggccaaggc ggtggccagg cagattgcgg
     3241 cgaccgaggc cgcgctggcg ccgcgcaaga gcgttgccgc gcgcgcgatg ctgcagcgcc
     3301 tgaagctcgc tcgc
//
LOCUS       DASUCY010000223         2691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1080877, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000223 DASUCY010000000
VERSION     DASUCY010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080877"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VFV25_04275"
     CDS             complement(<1..294)
                     /locus_tag="VFV25_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392563.1"
                     /translation="MAKWTAFPYDAAGYTHDAASLKKHWARLHAGDAEPFPKEAAAVE
                     AWALFHAGQFQKAVAAGLKAGGSGITAANKAQAIYANYLEKSEKTRLELFLEVA"
     gene            complement(328..996)
                     /locus_tag="VFV25_04280"
     CDS             complement(328..996)
                     /locus_tag="VFV25_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY1392564.1"
                     /translation="MSARSNKKEPRRTAERILEVTLDLFNRFGEPNVSTTLISAELNI
                     SPGNLYYHYPAKDELINSLFDRYERALSELLGAADNVKNVEDAWLFFHMFFELIWQYR
                     FLYRDLNDLLSKNRRLETHFQFVLKNKQQAVRSVLDGLSRSQNMRIDAREAEPVAAAM
                     VVVLTYWLSFEYVRDPRKALEPESAGAALMRGAYHVLSLLLPYLEASQKAHLLELVGR
                     YDKS"
     gene            1118..2059
                     /locus_tag="VFV25_04285"
     CDS             1118..2059
                     /locus_tag="VFV25_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY1392565.1"
                     /translation="MAAVSFQRVSKTYRSARGEVRALQDVSFDIESGEFFGLLGPNGA
                     GKTTLISILAGLSRASGGQVTVLGHDVVADYAAARKSLGIVPQELVFDPFFSVRETLR
                     IQSGYFGVRDNEAWIDELLENLGLADKANANMRQLSGGMKRRVLVAQALVHKPPVIVL
                     DEPTAGVDVELRQTLWQFIARLNKQGHTVLLTTHYLEEAEALCSRIAMLKQGRIVALD
                     RTTNLLAGTASTMLRFKTDHALPSTVAAHARVTGRIVQVTAHNPAEVESQLAALRTAG
                     VHIEDLEIGRADLEDVFLEIMQGHAEPAAEAVERRVA"
     gene            2056..>2691
                     /locus_tag="VFV25_04290"
     CDS             2056..>2691
                     /locus_tag="VFV25_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY1392566.1"
                     /translation="MSNDLALDGASTLFRKEILRFWKVSFQTVAAPVLTAVLYLLIFG
                     HVLENRVKVFGDIGYTSFLIPGLVMMSVLQNAFANSSSSLIQSKITGNLVFLLVTPLS
                     PWAWFVAYIGASVVRGLAVGLGVFLVTVWFAPPSLVEPWWILVFALLGAAMMGALGLV
                     AGLWAEKFDQMAAFQNFLIMPMTFLAGVFYSVHSLPGIWQAVSHLNPFFYMI"
BASE COUNT          420 a          865 c          881 g          525 t
ORIGIN      
        1 cgcgacctcg aggaagagtt cgagcctggt cttctcgctc ttctccaggt agttggcgta
       61 gatggcctgg gccttgttgg ccgccgtgat gccgctcccg cctgccttca ggcccgcggc
      121 cacggccttc tggaactgtc cggcgtggaa cagcgcccag gcttcgacgg cggccgcctc
      181 cttggggaag ggctcggcgt cgcccgcatg cagtcgcgcc caatgcttct tcagcgacgc
      241 ggcgtcgtgg gtgtagccgg cggcgtcgta ggggaaggcg gtccacttgg ccatgatgct
      301 tgtctccttg agtcttgttc ggcttgatca ggacttgtcg tagcggccca ccagttcgag
      361 cagatgggcc ttctgcgagg cttcgagata gggcagcagc aggctcagca catggtaggc
      421 gccgcgcatc aaggcggcac cggcgctctc gggctccagc gccttgcgcg ggtcgcgcac
      481 gtactcgaag ctcagccaat aggtcagcac cacgaccatc gcggccgcca ccggctcggc
      541 ttcgcgcgca tcgatgcgca tgttctgcga gcggctcaag ccgtcgagca cgctgcgcac
      601 ggcctgctgc ttgttcttga gcacgaactg gaaatgcgtc tccagccgcc ggttcttgct
      661 gagcaggtcg ttgagatcgc ggtacaggaa gcggtattgc cagatcagct cgaagaacat
      721 gtggaagaag agccacgcgt cctcgacgtt cttgacgttg tcggccgcgc cgagcagctc
      781 ggaaagcgcg cgctcgtagc ggtcgaacag gctgttgatc agctcgtcct tggctgggta
      841 gtggtaatag aggttgccgg ggctgatgtt cagctcggcc gagatcagcg tcgtcgagac
      901 attgggctcg ccgaagcgat tgaagaggtc gagcgtgacc tccaggatgc gttcggccgt
      961 gcggcgtggt tccttcttgt tgctcctcgc gctcatgttt tcccttatgt ctcctggtca
     1021 caatctagca tcgtcgggac acaccaccaa tggcggcggg gagggagtct cccctaggcc
     1081 tgcacgggcc gatgcggctg cctacaatgc ttcgcccatg gccgccgtct ctttccagcg
     1141 agtttccaag acctatcgca gcgcgcgcgg cgaagtgcgc gccctgcagg acgtgagctt
     1201 cgacatcgag tcgggcgagt tctttggcct cttgggtccc aacggcgccg gcaagacgac
     1261 gctgatcagc atcctcgcgg gcttgagccg cgccagcggc gggcaagtca cggtgctggg
     1321 ccatgacgta gtggccgact atgcggccgc gcgcaagtcg ctcggcatcg tgccgcagga
     1381 actggtattc gaccccttct tcagcgtgcg cgagacgctg cgcatccaga gcggctattt
     1441 cggcgttcgc gacaacgagg cctggatcga cgagctgctg gaaaacctcg gcctggccga
     1501 caaggccaat gccaacatgc gccagctctc gggcggcatg aagcgccgtg tgctggtggc
     1561 ccaggccctg gtgcacaagc cgccggtgat cgtgctcgac gagccgacgg ccggcgtcga
     1621 cgtggagctg cgccagacgc tgtggcagtt catcgcccgc ctcaacaagc agggccacac
     1681 cgtgctgctg accacccact acctcgagga agccgaggcg ctgtgctcgc gcattgcaat
     1741 gctcaagcag gggcgcatcg tcgcgctgga tcgcaccacc aacctgctgg ccggcaccgc
     1801 cagcaccatg ctgcgcttca agaccgatca tgcgctgccg tccaccgtcg cagcacacgc
     1861 tcgcgtgacc gggcgcatcg tgcaggtcac cgcccataac ccggccgagg tcgagtccca
     1921 gctcgccgct ttgcgcacgg ccggcgtgca tatcgaagac ctcgagatcg gccgcgccga
     1981 cctggaggac gtgttcctcg agatcatgca ggggcatgcg gagccggcgg cggaagcggt
     2041 ggaaaggagg gtggcatgag caatgacctc gcgctcgacg gcgcctcgac cctgttccgc
     2101 aaggaaatcc tgcgcttctg gaaggtgagc ttccagaccg tcgcggcacc ggtgctcact
     2161 gccgtgctct atctgctgat cttcggccat gtgctggaga accgcgtcaa ggtcttcggc
     2221 gacatcggct acaccagctt cctgatcccg ggcctggtga tgatgagcgt gttgcagaac
     2281 gccttcgcca acagctcgtc ttcgctgatc cagagcaaga ttaccggcaa cctggtgttc
     2341 ctgctcgtca cgccgttgag cccctgggcc tggttcgtag cctacatcgg cgcctcggtg
     2401 gtgcgcggtc tggccgttgg cctgggggtc ttcctcgtga ccgtgtggtt cgcgccgccc
     2461 agtctggtcg agccctggtg gatcctcgtg ttcgccctgc tcggcgcggc catgatgggg
     2521 gcgctcggcc tggtggcggg gctgtgggcc gagaagttcg accagatggc agccttccag
     2581 aattttctga tcatgccgat gaccttcctg gccggcgtgt tctattcggt gcattcgctg
     2641 ccggggatct ggcaggccgt gagccacctc aatcccttct tctacatgat c
//
LOCUS       DASUCY010000224         4589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1016740, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000224 DASUCY010000000
VERSION     DASUCY010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4589
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016740"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /gene="eutB"
                     /locus_tag="VFV25_04295"
     CDS             complement(<1..213)
                     /gene="eutB"
                     /locus_tag="VFV25_04295"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015001351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HEY1392567.1"
                     /translation="MAYAQTLGNRVYRFDDLKSLLALASPERSGDQLAGIAAESAEQR
                     MAARFALAELPLARFLSEPLIPYEEDE"
     gene            complement(275..1183)
                     /locus_tag="VFV25_04300"
     CDS             complement(275..1183)
                     /locus_tag="VFV25_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY1392568.1"
                     /translation="MTTDSSTTLPTAPAATLRTIALTVLAMLAFAANSLLCRLALQQP
                     VRIDPASFGSVRLVSGALMLALVLRLRAGGNGTQAAPATKPDWLAAAMLWTYVACFSF
                     AYLTLTAGTGALILFGAVQLTMFSVGLYAGERFAPAAWGGLLLAVAGLVYLVSPGLAA
                     PSPLGAALMATAGIAWGVYSLRGRRVAATPLAATASNFLRAAPLALGLSLAFITGFHA
                     DASGVVLAVASGAVTSGLGYVIWYAALTGLSAMRAATVQLSVPPIAALGGALLLAETI
                     TPRLVLASIAILGGVALVLLSRSRRS"
     gene            complement(1204..2037)
                     /locus_tag="VFV25_04305"
     CDS             complement(1204..2037)
                     /locus_tag="VFV25_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HEY1392569.1"
                     /translation="MSSSLAGRVAAGGGAAARHRRVFVPVLAALVALAWLVLWVWTRS
                     PYGRYLQHDGDWLAAGPVAALCRAVPGGTFLLPLGLTAAAWVLMTAAMMLPTTLPLFD
                     AFDRLATGRRDHARLLVLLGLGYLSAWGGFGLLAHGLHSLLLALVQAMPVLGGRSWLI
                     GAATLALAGAFQFSSLKHRCLDRCRTPLSFVMQHWRGRRQALQAFALGARHGLFCVGC
                     CWALMLLMFVVGAGSLGWMLVLAALMAVEKNFSWGQRISAPLGLALLAGSAALVLARL
                     G"
     gene            complement(2044..2646)
                     /locus_tag="VFV25_04310"
     CDS             complement(2044..2646)
                     /locus_tag="VFV25_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HEY1392570.1"
                     /translation="MSYHLEGRLLEVCNCRVLCPCWIGEDPDRGTCDTIVSWRMDKGT
                     IDGVDVGGVTIAAVAHVPGNILQGNWTAAIFVDDKASKAQEEALLKVYTGQAGGPIAD
                     LAKLIGTVVSVERAPIRFEVEGGKGTLAIGTDYYAELEPYIGPSGKTTTLSDTVFSTV
                     PGAPVFVGKAPIYRSKNAKIGIDVNISGHNALQSTFVFDA"
     gene            complement(2814..3443)
                     /locus_tag="VFV25_04315"
     CDS             complement(2814..3443)
                     /locus_tag="VFV25_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HEY1392571.1"
                     /translation="MAKTSRNAHALLAAAMLLALHPSAAPAQLQAQAQPAAAAAAPPR
                     DGSHDFDFEIGEWLTHLKRLQRPLSGSTTWVEYEGTSSVRGVLGGRANLVELVADGPA
                     GRIEGASLRLYDPQAHQWTLNYFNIADGHLTAPMVGEFIDGKGMFRAQDSYKGQPILV
                     RFVIARVNENVYRFEQAFSPDGGQTWETNWVATDTRLSSRSTGMRGRSL"
     gene            3637..4329
                     /gene="lipB"
                     /locus_tag="VFV25_04320"
     CDS             3637..4329
                     /gene="lipB"
                     /locus_tag="VFV25_04320"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEY1392572.1"
                     /translation="MPDAIALQALVRKLGRVDYAETVAAMRAFTEARDADTPDEIWLC
                     EHAPVYTQGIAGRAEHVLDAHGIPVVQTNRGGQVTYHGPGQVVAYPLIDLRRLRIYVK
                     EYVFRLEQAVLKTLESYGVTGHRVAGAPGIYVRLDDPRSHAALAGPQPGGDPFHGLGK
                     IAALGVKLSQHRSYHGVALNVAMDLKPFADINPCGYPGLATVDLSTIGVLTDWDDAAE
                     RLGARLASYLSP"
     gene            4357..>4589
                     /locus_tag="VFV25_04325"
     CDS             4357..>4589
                     /locus_tag="VFV25_04325"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006682025.1"
                     /note="catalyzes the radical-mediated insertion of two
                     sulfur atoms into an acyl carrier protein (ACP) bound to
                     an octanoyl group to produce a lipoyl group; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEY1392573.1"
                     /translation="MSAENLAADAQQNGAAAYDPTIKQKAQAKTARIPIKVVAAETLK
                     KPDWIRVRAGSPTTRFYEIKQILREHKLHTVCE"
BASE COUNT          791 a         1586 c         1573 g          639 t
ORIGIN      
        1 ctcgtcctct tcatagggaa tcagtggctc gctgagaaag cgggccagtg gcagctcggc
       61 caatgcgaac cgggccgcca tgcgttgctc ggccgactct gcagcgatgc cggccagctg
      121 gtcgcccgag cgctcgggcg aggctagtgc gagcagcgac ttcagatcgt cgaagcgata
      181 gacgcggttg cccagcgttt gggcataggc catggccgaa ctcccgagcg gtgaggatgc
      241 cgtggccccc agtatgaggc cggccgcgga gaaatcagga tctgcgcgag cggctcagca
      301 gcaccagggc cacgccgccg aggatggcga tcgaggccag caccaggcga ggcgtgatcg
      361 tctcggccag cagcagcgcg ccgccgaggg ccgcgatggg cggcaccgag agctgcaccg
      421 tcgcggcgcg catggcgctc aagcccgtga gggcggcata ccagatcaca tagcccaggc
      481 ccgaggtcac ggcgccggaa gcaacagcca gaacgacgcc gctcgcgtcg gcgtgaaagc
      541 cggtgatgaa ggccaggctt agccccaagg ccaggggcgc ggcgcgcagg aagttggacg
      601 ccgtggcggc cagcggggtc gcggcgacac gccgcccgcg cagcgaatac acgccccagg
      661 cgatgccggc cgtggccatc agcgcggcgc ccagcggcga gggcgcggcc agcccgggcg
      721 agacgagata gaccaggccc gccaccgcca gcagcaggcc gccccaggcc gcgggcgcga
      781 agcgttcgcc ggcatagagc ccgacgctga acatcgtgag ctgcaccgcg ccgaacagga
      841 tcagcgcgcc ggtgccggcc gtcagcgtga gataggcgaa ggagaagcag gccacatagg
      901 tccacagcat ggcggcggcg agccagtcag gcttcgttgc cggcgcggcc tgggtgccgt
      961 tgccgccggc tctcagcctc agcaccagcg ccagcatcag ggcgcccgac accagccgca
     1021 cgctgccgaa gctggccggg tcgatgcgca ccggctgctg cagcgccagc cggcacagca
     1081 gcgaattggc cgcgaaggcg agcatcgcca acacggtgag cgcgatggtg cgcagcgtcg
     1141 cggccggtgc cgtggggagc gtggtggacg agtcggttgt catctgtgcc ttttcgccgg
     1201 cgatcatccc agcctggcca gcaccaatgc ggccgagccg gccagcagcg ccagcccgag
     1261 cggcgcgctg atgcgctgcc cccaggagaa attcttctcc accgccatca gcgccgccag
     1321 caccagcatc cagcccagac tgccggcgcc gaccacgaac atcagcagca tcagtgccca
     1381 gcagcagcct acgcagaaaa ggccatggcg ggcgcccagc gcgaaggcct gcagcgcctg
     1441 ccggcgcccg cgccaatgct gcatcacgaa gctgagcggc gtgcggcatc ggtcgaggca
     1501 gcggtgcttg aggctgctga actgaaaggc gccggccagc gcgagcgtgg ccgcgccgat
     1561 cagccagctg cgcccgccca gcaccggcat cgcctgcacc agcgccagca gcaggctgtg
     1621 caggccatgt gccagcaggc cgaagccgcc ccaggccgag agatagccca ggcccagcag
     1681 caccagcagg cgggcatggt cgcgccgccc ggtggcgagg cggtcgaagg cgtcgaagag
     1741 gggcagcgtg gtcggcagca tcatggcggc cgtcatcagc acccaggccg cggcggtcag
     1801 cccaagcggc aagaggaaag tgccgcccgg cacggcgcgg cacagcgcgg cgacgggccc
     1861 cgccgccagc cagtcgccgt cgtgctgcag atagcgtcca taggggctgc gcgtccagac
     1921 ccacagcacc agccaggcca gcgcgacgag cgctgccagc accggcacga agacgcgcct
     1981 atggcgcgca gccgcgccgc cgccggccgc gaccctgccg gccaagctgc tggacatacc
     2041 gcttcaggcg tcgaagacga aggtgctctg cagcgcgtta tgccccgaga tgttcacatc
     2101 gatgccgatc ttggcgttct tcgagcggta gatgggcgcc ttgccgacga acaccggcgc
     2161 gccgggcacg gtcgagaaca cggtgtccga cagcgtcgtc gtcttgccgc tgggcccgat
     2221 atagggttcg agttcggcgt agtaatcggt gccgatggcc agcgtgccct tgccgccttc
     2281 cacctcgaac cggatcggcg cgcgctccac cgataccact gtgccgatca gcttggcgag
     2341 gtcggcgatc ggcccgccgg cctggccggt gtagaccttg agcaaggcct cttcctgtgc
     2401 cttggaggcc ttgtcgtcga cgaagatcgc ggcggtccag ttgccctgca ggatgttgcc
     2461 gggcacatgg gccacggcgg cgatggtcac gccgcccacg tcgacgccat cgatcgtgcc
     2521 cttgtccatg cgccaggaca cgatggtgtc gcaggtgccg cggtcgggat cctcgcctat
     2581 ccagcagggg cacaagaccc tgcagttgca gacttccagg agacgacctt caaggtggta
     2641 gctcatatga tgaccctcca tccgaccagc agaaagccag ttcgacgcac ccacgccacg
     2701 cccgcggcat ggaatcgatg atgctacgcc gcggctgcgg ctgggtccag gaggcctagc
     2761 gtattcgtgg ggggcatcgg ccaggggccg gcgaggcacc gctgactcgc ggctcagagg
     2821 ctgcggcccc tcatgccggt gctgcgcgag gacaaccgcg tgtcggtcgc gacccagttg
     2881 gtctcccagg tctggccgcc gtcgggagaa aacgcctgct cgaagcggta gacgttctcg
     2941 ttcaccctgg cgatcacgaa gcgcaccagg atgggctgcc ccttgtagct gtcctgcgca
     3001 cggaacatgc ctttgccgtc gatgaattcg ccgaccatcg gcgccgtcag atggccgtcg
     3061 gcgatgttga agtaattgag tgtccactgg tgcgcctgcg gatcgtagag gcgcagcgat
     3121 gcgccctcga tgcgccccgc cggcccatcg gcgaccagct cgaccagatt ggcacggccg
     3181 ccgagcacgc cccgcaccga gctcgtcccc tcgtactcga cccaggtggt cgagcccgac
     3241 agcgggcgct gcagccgctt gaggtgggtg agccattcgc cgatctcgaa gtcgaaatcg
     3301 tggctgccgt ctcttggcgg cgccgcggcc gctgcggccg gctgcgcctg ggcctgcagc
     3361 tgagccgggg ctgcgctggg gtgcagcgcc aggagcattg cggccgcgag cagggcatgg
     3421 gcatttctgc ttgtctttgc catggatctg cctccttggg atgtgggccg cagtgtctca
     3481 gagatgacct gtccttgcag gccattcggt gccactcgga tgctacacaa tcagtactga
     3541 aaatcatcaa aatagcttcg gctgccggat gcaaaaactg caatccgtct tgcccatgcg
     3601 gcgcaccggc ctcgggcggc gcgccacaat cgcgacatgc cggatgccat cgccctgcaa
     3661 gccctcgtgc gcaagctcgg gcgcgtcgac tatgccgaaa cggtggccgc catgcgcgcc
     3721 ttcaccgagg cgcgcgacgc cgacacgccg gacgagatct ggctgtgcga gcatgcgccg
     3781 gtctacaccc agggcatcgc cgggcgtgcc gagcatgtgc tcgacgccca cggcatccct
     3841 gtggtgcaga ccaaccgcgg cgggcaggtg acctatcacg ggccggggca ggtggtggcc
     3901 tatccgctga tcgacttgcg gcggcttcgc atctacgtca aggaatacgt cttccgcctc
     3961 gagcaggcgg tgctcaagac gctggagagc tacggcgtca ccggccatcg cgtggccggg
     4021 gcgccgggca tctatgtgcg gctggacgat ccgcgctcgc acgccgcgct ggcggggccg
     4081 cagccgggcg gcgacccctt ccatggcctc ggcaagatcg cggccctggg cgtcaagctc
     4141 agtcagcacc gcagctatca cggcgtggcg ctcaatgtcg cgatggacct gaagcccttt
     4201 gccgacatca acccttgcgg ttacccgggg ctcgccacgg tcgacctttc tacaatcggg
     4261 gttctcaccg attgggacga tgcggccgag cgcctggggg cgcggctggc gagctatctg
     4321 agtccctgaa cgcgcgcccc accggagttc ccgcacatgt ccgcagaaaa cctggccgcc
     4381 gatgcccaac aaaacggcgc cgctgcctac gatcccacca tcaagcagaa ggcccaggcc
     4441 aagacggcgc gcatcccgat caaggtggtg gctgccgaaa cgctgaagaa gcccgactgg
     4501 atccgcgtca gggccggctc gccgaccaca cgtttctacg agatcaagca gatcctgcgc
     4561 gagcacaagc tgcacaccgt ctgcgagga
//
LOCUS       DASUCY010000225         4309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_20741, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000225 DASUCY010000000
VERSION     DASUCY010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20741"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..1561
                     /locus_tag="VFV25_04330"
     CDS             152..1561
                     /locus_tag="VFV25_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006481325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HEY1392574.1"
                     /translation="MTSTSTIEGTAPALPKKTLPWYRKLGMQVLVSLILGIAVGFVFP
                     KFASQLKLLGDMFLSLIKAGVAPLVFLTIVHGIASAGDVRSAGRVGWRSIVYFEIVST
                     VALMLGLLAGNVLQIGRGMSSVLAVSVPAAAKTAAPQGFLEFMAHIVPDNFVGAFAKG
                     ELLQVVVLAVMVGIGILAIPIERREKITAGLDHVSEVLFSFINLVMKLAPIGTFGAVA
                     YSVGSNGTTVLVALAQLVLGFYALVVVFIAVALGAIAWLSGFSLWRFLRYIKDEILIV
                     LGTASSESALPRLLIKLERLGCAKQTVGLVLPTGYAFNLDGTSLFMAMGVMFISHAYG
                     VPLSFDQQLGILLLMLLTSKGAATVSGGSFVVFAATVTSTGILPIEGLAVIFGVYRFL
                     SMAIATCNTIGNSVATVVVAKWSGTFDAYTAARHLYPERHPETAAADAALDDGNLLEE
                     DANERNAHPQGIALAKPSA"
     gene            complement(1585..4287)
                     /locus_tag="VFV25_04335"
     CDS             complement(1585..4287)
                     /locus_tag="VFV25_04335"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HEY1392575.1"
                     /translation="MEHGELLPEDGRKTTESGWRWRVGKLELANGRVLLHDPAWPQAQ
                     AIAPIQASVAGLDSAASAAPAKLALSLADAQGALIKIDGTLNAAARSAKLKAEVAGLK
                     PAPWLGPWQALLPVRVLDGSLALQAQLEADPKSWALQEGALQLSGLQLEPSAGSAAKP
                     SPADKKPGNKRGAATDLLNLPKLEASGVQVHAPSGEALKAQVAALRFEGLDLKLSRGE
                     HGEIAWLPPTKPTAAGESKRLRNEKPGPEPQWQIGELQCSACAVALSDRSVQPAAALS
                     LTRTDFTLRKLGSDAKQPANFELALQAGHGGSAKARGELRLQPLALQSHMELAGLDLS
                     VLQPYLDPYLNLTLRSAKAHAAGDLRIDGGAQGGIDVARWRGRMALRELRAVDKINSA
                     EFLRFKNLSLDGADLAWKPAGITADLGTISLDSFYGRVILNADARLNLRDVVKREGRE
                     PARSITTPTGPEAAASAAQPAAAAAPAPQAASTPAPEAASAPAPAASTASAPAAAGPP
                     PQLRWKAIHLTAGQIDFTDNFVRPNYSAQLTDVAGEVSGVAWNDPQPANVKISGKVDG
                     SAPLEISGTVHPLGARLQTDITANARGIDITRLSTYSARYAGYGIDKGTLSVRVHYKI
                     DNGKLEAQNNLHLDQLTFGGKVDSPNALKLPVLLAVSLLKDRNGVIDVDLPVSGSLDD
                     PQFSIGGVIARVIVNLIAKALTAPFSLLSHAFSHGSAEELGYIEFAPGTAELSEASIK
                     RLDALTEALNDRPTLKLEVTGRTDPAVDTEGLRQVHVEHLMLAAKAKATGELRDSVTI
                     APAERERWLAAAYKDADIKGKPRNLIGLQKSLPAAEMQALLEQSANAGPTQLGRLADE
                     RGDRVKAYLTAKVAPERVLLTASKLGTDGISDQGKTSRVSFDLK"
BASE COUNT          593 a         1443 c         1501 g          772 t
ORIGIN      
        1 ccctgcccga ggtcacgaag cacttcgacc gcgccaccat cgatcgcctc accaaccccg
       61 ctaactacct cggtcttgcg ccgcagatgg tggatcgcgc gctggcgctg tccgccgaga
      121 tcagcgcctg aggcgcatgg agagagacaa catgacaagc acatccacca tcgaggggac
      181 cgcgccggcg ttgccgaaga agacgctgcc ctggtatcgc aagctcggca tgcaggtgct
      241 ggtctcgctg atcctcggca tcgccgtcgg cttcgtcttt ccgaagttcg cgtcccagct
      301 gaagctgctc ggcgacatgt tcctgtcgct gatcaaggcc ggcgtcgcgc cgctggtgtt
      361 cctcaccatc gtgcacggca tcgcatcggc cggcgacgtg agaagcgcag ggcgcgtggg
      421 ctggcgctcc atcgtctatt tcgagatcgt ttcgaccgtc gcgctcatgc taggcctgct
      481 cgcgggcaac gtgttgcaga tcggccgggg catgtcgtcg gtcttggccg tgtccgtgcc
      541 ggccgcggcc aagaccgcgg cgccgcaggg cttcctcgag ttcatggccc acatcgtgcc
      601 ggacaacttc gtcggtgcct tcgccaaagg cgagctgctg caggtcgtcg tgctggccgt
      661 gatggtgggc atcggcatcc tggcgatccc catcgagcgg cgtgaaaaga tcaccgccgg
      721 actcgaccat gtctccgagg tgttgttctc cttcatcaac ctggtgatga agcttgcgcc
      781 gatcggcacc ttcggcgccg tggcctactc ggtgggcagc aacggcacga cagtcctggt
      841 agcgcttgcg caactggtgc tcggcttcta tgcgctggtg gtcgtcttca tcgccgtcgc
      901 gctcggcgcc atcgcgtggc tgtccggctt cagtctgtgg cgcttcctgc gctacatcaa
      961 ggacgagatc ctcatcgtgc tcggcacggc ctcctcggaa agcgcgctgc cgcgcctgct
     1021 gatcaagctg gagcgcctgg gctgtgccaa gcagacggtg gggctggtgc tacccacggg
     1081 ttacgcgttc aacctggacg gcacatcgct gttcatggcg atgggcgtga tgttcatctc
     1141 gcacgcctac ggcgtcccgc tgtcgttcga ccagcagctg ggcatcctgc tgctgatgct
     1201 gctgacctcc aagggcgcgg ctacggtctc gggcgggtcc ttcgtcgtgt tcgccgcaac
     1261 cgtcacgtcc accggcatcc tgccgatcga aggcctcgcc gtcatcttcg gcgtctaccg
     1321 cttcctgtcg atggccatcg caacctgcaa caccatcggc aacagcgtgg cgacggtcgt
     1381 cgtcgccaag tggtccggca ccttcgatgc ctacacggcg gctcgccacc tgtaccctga
     1441 gcgccacccc gagaccgctg cagcagacgc cgcactggac gacggcaacc tgctcgagga
     1501 agacgccaac gagcgcaatg cgcaccccca gggcatcgcc ctcgccaagc catccgcctg
     1561 agccggtgcg gtgaatgccg ggggctactt caaatcaaag gagacgcgcg aggtcttgcc
     1621 ctggtcgctg atgccatcgg tgcccagctt ggaggcagtg agcagcaccc gctcgggcgc
     1681 caccttggcc gtcagatagg ccttgacgcg gtcgccgcgc tcgtcggcga gcctgccgag
     1741 ctgggttggg ccggcgttgg cgctttgctc cagcagggcc tgcatctcgg cagccggcag
     1801 gctcttctgg aggccgatga ggttgcgcgg cttgcccttg atgtcggcgt ccttgtaggc
     1861 ggctgctagc cagcgttcgc gctcggccgg cgcgatcgtc acgctgtcgc gcagctcgcc
     1921 ggtcgccttg gccttggcgg cgagcatcag gtgctcgacg tggacctggc gcaggccctc
     1981 ggtgtcgacg gccgggtcgg tgcggccggt cacttcgagc ttgagcgtgg gccggtcgtt
     2041 gagcgcctcg gtcagggcat ccaggcgctt gatgctggcc tcgctcagct ccgccgtacc
     2101 gggcgcgaat tcgatatagc cgagctcctc tgcgctgcca tgcgagaagg catgcgacag
     2161 cagcgagaag ggcgcggtca acgccttggc gatgaggttg acgatgacgc gcgcgatgac
     2221 gccgccgatg gagaactgcg ggtcgtccag gctgccgctc accggcagat cgacatcgat
     2281 cacgccattg cggtccttga gcagcgagac agccagcagc accggcagct tgagcgcatt
     2341 ggggctgtcg accttgccgc cgaaggtgag ctggtcgagg tggaggttgt tctgggcctc
     2401 gagcttgccg ttgtcgatct tgtagtggac cctgaccgac agcgtcccct tgtcgatgcc
     2461 gtagccggca tagcgggccg agtaggtgct cagccgcgtg atgtcgatgc cgcgcgcgtt
     2521 ggccgtgatg tcggtctgca gccgcgcgcc cagcgggtgc acggtgcccg agatctccag
     2581 cggcgcgctg ccgtcgacct tgcccgagat cttcacattg gccggctgcg ggtcgttcca
     2641 ggccacgccg gagacttcgc cagccacgtc ggtgagctgc gccgaatagt tggggcggac
     2701 gaagttgtcg gtgaagtcga tctggccggc cgtgaggtgg atggccttcc agcgcagctg
     2761 gggcggcggg ccggcagcgg cgggagcgct ggcagtggat gctgcaggcg caggcgcgga
     2821 agccgcctcc ggcgcgggtg tggaagccgc ctgcggcgca ggtgcggcag cagccgcagg
     2881 ctgagcagcg ctggccgcgg cctcggggcc ggttggcgtg gtgatggagc gcgccggctc
     2941 gcggccctcg cgcttgacga cgtcgcgcag gttgagccgc gcatcggcat tgaggatcac
     3001 gcggccgtag aagctgtcga gcgaaatcgt gccgaggtcg gccgtgatgc cggccggctt
     3061 ccaggccaga tcggcgccgt cgaggctgag gttcttgaag cgcaggaact cggccgagtt
     3121 gatcttgtcg accgcgcgca gctccctgag cgccatgcgg ccgcgccagc gtgccacatc
     3181 gatgccgccc tgcgcgccgc cgtcgatgcg caggtcgccg gccgcgtgcg ccttggccga
     3241 gcgcagcgtg aggttcagat aggggtcgag atagggctgc agcacgctca ggtccagccc
     3301 ggccagctcc atatggcttt gcagcgctag cggctgcagg cgcagctcgc cgcgcgcctt
     3361 ggcgctgccg ccgtggccgg cctgcagcgc cagctcgaag ttcgccggct gcttggcatc
     3421 gctgccgagc ttgcgcagcg tgaagtcggt gcgcgtgagc gagagcgcgg ccgcgggctg
     3481 cacgctgcgg tcgctgagcg ccacggcgca ggcgctgcat tgcagctcgc cgatctgcca
     3541 ttgcggctcg gggccgggct tctcgttgcg caagcgcttg ctttcgccgg ccgccgtcgg
     3601 cttggtcggc ggcagccagg cgatctcgcc atgctcgccg cgcgagagct tgaggtccag
     3661 gccctcgaag cgcagcgccg cgacctgcgc cttcagcgct tcgccgctgg gcgcatgcac
     3721 ctgcacgccg cttgcttcga gcttgggcag gttcagcagg tcggtggccg cgccacgctt
     3781 gttgccgggc ttcttgtccg ctggcgatgg ctttgcggcc gagcccgccg acggctccag
     3841 ctgaaggccg ctgagctgca aggcgccctc ctgcaaggcc cagctcttgg ggtcggcctc
     3901 aagctgagcc tgcagcgcga ggctgccgtc gagcacgcgc accggcagca gcgcctgcca
     3961 gggcccgagc cagggcgcgg gcttgaggcc ggccacctcg gccttcagct tggcgctgcg
     4021 cgcggccgcg ttcagcgtgc cgtcgatctt gatcagcgcg ccctgggcat cggccagcga
     4081 cagggcaagt ttggccggcg cggcgctggc ggcggaatcc agcccggcga cgctggcctg
     4141 gatgggcgca atggcttgcg cctgcggcca agcggggtcg tgcagcagca cgcggccatt
     4201 ggccagctcg agcttgccga cccgccatcg ccagccggat tccgtagttt ttctgccatc
     4261 ttccggaagt aattcgccat gttccacatc agaatttgta gcagtcttt
//
LOCUS       DASUCY010000226         3594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1466385, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000226 DASUCY010000000
VERSION     DASUCY010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1466385"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..558
                     /locus_tag="VFV25_04340"
     CDS             115..558
                     /locus_tag="VFV25_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEY1392576.1"
                     /translation="MAQSNATQVQAKASITRDSALALAIAARDAAIKIGLNLAIAVTD
                     EGGHLIAFERADATPFLAAEVAINKAWTAASFGLDTMIWNQVVAQPATAPLANHPRVM
                     PVGGGAPIVVGGRVVGGIGISGGTAQQDHDVASAALKAAGFEVAK"
     gene            555..1004
                     /locus_tag="VFV25_04345"
     CDS             555..1004
                     /locus_tag="VFV25_04345"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY1392577.1"
                     /translation="MNATISRRDAGVVLAAVALAASAAGRAAADVDVASAAVDLYARM
                     TARDLAGALRYIPAAGFTEIGPSAPSVHVLKPAAFEALFNSDAVIKLRAEDVNVHRFG
                     STAVVTGVRVGSIGPKDKPSTELRAPFTMVWTSNNGSWQLRHIHLSS"
     gene            complement(1265..3217)
                     /locus_tag="VFV25_04350"
     CDS             complement(1265..3217)
                     /locus_tag="VFV25_04350"
                     /inference="COORDINATES: protein motif:HMM:NF015585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HEY1392578.1"
                     /translation="MKKTVLPLLLALLAASAHAEVRLANVFGEHMVLQRDRPLQLWGQ
                     ATPGQTLSVELAGRKASARVGADGRWRVQLAQLPAGGPHRLLVKGDQTVELRDVLIGD
                     VWLLGGQSNMEWPLAQTDTGPQEVASPQNPQLRQLRVPLRASVRPEAEIAPAPWVVAE
                     PGRVGEFSAIGYHFARQMQAVQGVPIGLIQTAWGGSMLETWVRRDAALQDPDLAPAVH
                     ALPPDNAAFIVTLRQRLYDRVAAWQPGLPLQGVDASGWSAAEDIDANWPTLQAPGVWE
                     GQGLADLDGVVWMRKRVELNAAQAAGAAELYLVKVDDCDETWVNGQRVGGQCGWEQPR
                     RYALPAGLLRAGTNWIAVRVTDTGGNGGFHGDAAALRLDTAAGSVSLAGAWRARVEQI
                     AAAAGPVANDAPALGHNGLIAPLHGLAVRGVLWYQGEENSGRAAAYADGFKRLIQDWR
                     AQFGDPNLPFFFVQLAAWLPLADNRPDGNGWAELRASQAAALALPHTGMATAIDVGDA
                     VDIHPRNKRTLGQRLAALALHELGLRAAPASGPRLLGHEVKGGEFQLSFGDTAGGLRT
                     ARAGEPPRGFYLAGADRHWVPAEGRFDGNRIVLHSDAVPAPVAARYAWVNNPSEANIV
                     GGDGLPLPPLRTDDWPLESAGRRYQL"
     gene            complement(3246..>3594)
                     /locus_tag="VFV25_04355"
     CDS             complement(3246..>3594)
                     /locus_tag="VFV25_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HEY1392579.1"
                     /translation="TAEVLSRFSNLDGTPPAITVNKFGKGRAIYLATPAQPQIMRPLL
                     RSLYASLGIVPGPKTPDGVFAREVEGRVLYVNTNWHPVDVPIAGAMKGVLGGQRWEGT
                     LRLERLGAELLER"
BASE COUNT          516 a         1232 c         1266 g          580 t
ORIGIN      
        1 tgggcaagat cgtgctcgaa ggcttctgat ttcgagcgca cctcatacaa gtacccaggc
       61 gcacaacttc aattctttca cccctccgtt aacttcatct caaggaaact catcatggct
      121 caaagcaacg ctacccaagt ccaagccaag gcttccatca cgcgcgacag cgcgctggcg
      181 ctggccatcg ctgcgcgcga cgcggccatc aagatcggcc tgaacctggc cattgcggtc
      241 accgacgaag gcggccacct gatcgccttc gagcgcgccg acgccacgcc gttcctggcc
      301 gccgaagtgg ccatcaacaa ggcctggact gccgcgtcct tcggtctgga cacgatgatc
      361 tggaaccagg ttgtcgctca gccagccacg gctccgctgg ccaatcaccc gcgcgtcatg
      421 cctgtgggcg gcggtgctcc catcgtggtg ggcggtcgtg tggttggcgg catcggcatc
      481 tctggtggca ccgcccagca agaccacgac gtggcttccg ctgcgctcaa ggccgctggc
      541 ttcgaggttg ccaagtgaac gcaaccatct ctcggcgcga cgccggcgtc gtgttggctg
      601 cggtggcact ggcggcctcg gcggcgggca gggcggccgc tgatgtcgat gtcgcttctg
      661 ccgcggttga cctctacgcg cggatgactg cacgggatct ggctggcgcc cttcgctaca
      721 tccctgctgc cggattcacc gagatcggcc cgtcggcacc ttcggttcac gtcctgaagc
      781 ctgcagcgtt tgaggcgctg ttcaattcgg atgcggtgat caagctccgc gccgaagacg
      841 tgaacgtcca ccgcttcggt agcaccgcgg tcgtaacggg cgtacgtgtt ggctcgatag
      901 gcccgaagga caagccgtct actgagcttc gggcgccgtt cacgatggtt tggaccagca
      961 acaacggtag ctggcagctg cgacacatcc atctgtcttc ctgagacagt ggggcgatgt
     1021 gggaacacat tgctaaaccg tagagagaag ggccacccct caatcggggt agctctcccg
     1081 acattagggc ctgttaacac taattgctaa ggtggtggtt cctcatcggt aagcgatcaa
     1141 tagccttcgc cggctgtgtt caggtgaccg ccatgcaggg atcgtggcgt gatcggacat
     1201 cgctggccgg ggaccgccac aagcacggtc tcggcggcgc gtagaggtct tcgcttgccc
     1261 agactcagag ctggtagcgc cgtccggccg attccagcgg ccagtcgtcg gtgcgcagcg
     1321 gcggcagggg caggccatcg ccgccgacga tgttggcctc gctcgggttg ttgacccacg
     1381 catagcgcgc cgccaccggt gccggcacgg cgtcgctgtg cagcacgatg cggttaccgt
     1441 cgaagcggcc ttccgccggc acccagtggc ggtcggcgcc ggctaggtag aagccgcgcg
     1501 ggggctcgcc ggcgcgggcg gtacgcaggc cgccggcggt gtcgccgaag ctcagctgga
     1561 actcgccgcc cttgacctcg tggccgagca ggcgcgggcc gctggccggc gcggcgcgca
     1621 ggcccagctc gtgcagggcc agtgcggcca ggcgctggcc gagagtgcgc ttgttgcgcg
     1681 gatggatgtc gaccgcgtcg cccacgtcga tggcagtggc catgccggtg tggggcaggg
     1741 ccagggctgc ggcctggctc gcgcgcagtt cggcccagcc gttaccgtcg ggccggttgt
     1801 cggccagcgg cagccaagcc gccagctgca cgaagaagaa gggcaggttg gggtcgccga
     1861 actgggcgcg ccagtcctgg atcaggcgct tgaagccgtc ggcataggcg gccgcgcgtc
     1921 cggaattttc ctcgccctgg taccagagca cgccgcgcac ggccagtccg tgcagaggtg
     1981 cgatcaggcc gttgtggccc agggccggtg cgtcattggc gacgggtccg gccgctgccg
     2041 cgatctgctc cacgcgcgcc cgccaggcgc cggccagcga gacgctgccg gccgcggtgt
     2101 ccagacgcag tgcggcggcg tcgccgtgga agccgccgtt accgccggtg tcggtcacgc
     2161 gcacggcgat ccagttcgtg cctgcgcgca gcaggccggc gggcagggcg taacggcgtg
     2221 gctgctccca gccgcactgg ccgccgacgc gctggccgtt gacccaggtc tcgtcgcagt
     2281 cgtccacctt gaccaggtac agctcggccg caccggcggc ctgggcggcg ttcagttcca
     2341 cccgcttgcg catccagacc acgccgtcca ggtcggccag gccctgccct tcccacacgc
     2401 ccggggcctg cagcgtgggc cagttggcgt cgatgtcctc ggcggcggac cagccgctgg
     2461 cgtccacgcc ctgcagcggc aggcccggct gccatgcggc cacgcggtcg tacaggcgct
     2521 ggcgcagcgt gacgatgaag gcggcgttgt cgggcggcaa tgcgtgcacc gcgggcgcga
     2581 ggtcggggtc ctgcagcgcg gcgtcgcggc gcacccaggt ctccagcatc gagccgcccc
     2641 aggcggtctg gatgaggccg atgggcacgc cctgcaccgc ctgcatctgg cgcgcgaagt
     2701 ggtagccgat ggcgctgaac tcgcccacgc ggccgggctc ggctaccacc cagggtgcgg
     2761 gcgcgatctc ggcctcgggc cgcacgctgg cgcgcagcgg cacgcgcaac tggcgcagct
     2821 gcgggttctg cggcgacgcc acttcctggg gaccggtgtc ggtctgcgcg agcggccact
     2881 ccatgttcga ttggccgccg agcagccaca cgtcgccgat cagcacgtcg cgcagctcga
     2941 cggtctggtc gcccttgacg agcaggcggt gcgggccgcc ggcgggcagc tgagcgagtt
     3001 gcacacgcca gcggccatcg gcgccgacgc gcgcgctggc cttgcggccg gcgagttcga
     3061 cgctcagcgt ctggccgggc gtggcctggc cccagagctg caggggccgg tcgcgctgca
     3121 gcaccatgtg ctcgccgaac acgttggcga ggcgcacttc ggcgtgcgca gaagcggcca
     3181 gcaaggccag caagaggggg aggacggtct tcttcatagc gttcttcatc gggtttcctg
     3241 tcgggtcagc gttccagcag ttccgcgccg agccgctcca gccgcagcgt gccttcccag
     3301 cgctggccgc cgagcacgcc cttcatcgcg ccggcgatcg gcacatccac cggatgccag
     3361 ttggtgttga cgtagagcac gcggccttcg acctcgcgtg cgaacacgcc atcgggcgtc
     3421 ttcggtcccg gcacgatgcc gaggctggcg tagagcgagc gcagcagcgg ccgcatgatc
     3481 tgcggctgcg ccggcgtcgc gaggtagatc gcgcgcccct tgccgaactt gttgacggtg
     3541 atcgccggcg gcgtgccgtc gaggttgctg aagcggctca gcacctcggc ggtc
//
LOCUS       DASUCY010000227         2728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_406180, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000227 DASUCY010000000
VERSION     DASUCY010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406180"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="VFV25_04360"
     CDS             <1..682
                     /locus_tag="VFV25_04360"
                     /EC_number="5.4.2.2"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205789.1"
                     /note="catalyzes the interconversion of alpha-D-mannose
                     1-phosphate to alpha-D-mannose 6-phosphate and
                     alpha-D-glucose 1-phosphate to alpha-D-glucose
                     6-phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEY1392580.1"
                     /translation="FDGDGDRLGVVTTDGQIIYPDRQLMLFAQDVLSRVPGGQIIYDV
                     KCSQRLATAVREAGGKPLMWKTGHSLIKAKLKETGAPLAGEMSGHIFFAERWYGFDDG
                     MYTAARLLEILSRYDDPSALLNALPTSHSTPELNVACAEGEPHALIEKLRAVASFEGA
                     RELVTIDGLRADYEDGFGLIRASNTTPVLVLRFEGHTPQALERIQGQFMAALHAVKPD
                     ARIAAAAH"
     gene            724..1752
                     /gene="waaC"
                     /locus_tag="VFV25_04365"
     CDS             724..1752
                     /gene="waaC"
                     /locus_tag="VFV25_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HEY1392581.1"
                     /translation="MRVLIVKLSSLGDVVHAMPVVHDIRRARPGAAIDWVVEPGFAPL
                     VRRVDGVREVIECAVRRWRKAWWTSAVRTEWRAFRERLQRERYDAVLDLQGLTKSALI
                     ARLASVDAVGHRYGLGNRTDGAGWEPTARWLVDRPIFVAPRTHALDRSRLLAAQALGY
                     ELEASPVFGLNAQAKPYSPPVVAFVHGSSREDKLWPEAHWIELGRRLIDESYGIALPQ
                     GSEDERRRAERIADALGPAAKVWPGMGLDTVVDCLAGCTGVIGVDSGLSHIAVALDLP
                     HVQLYNFKTAWRTGPQRSHGHEHQVSIGGDAPPGLEEVWQAWLEVRRPGREVDETERD
                     TQPASFTP"
     gene            1749..>2728
                     /locus_tag="VFV25_04370"
     CDS             1749..>2728
                     /locus_tag="VFV25_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HEY1392582.1"
                     /translation="MKPPPSRLRSSLARRAYSTLLQLLKPFYVARLWWRGRAEPLYRH
                     RVAERLGAYRGARSEGWIWLHAVSLGETRAAATLIAALRERKPGMKLLLSHGTATGRT
                     AGQELLADGDQQVWLPYDTPGVVQRFFSQFRPAVGVLMETEVWPNLLRGAQRAGVPML
                     LANARLSERSLSKALRLDAVMRPAAASLSAVLAQSEADARRLKLAGATRVQVCGNLKF
                     DITPDAGLLARGQRWRAASIRPIVLAANTREGEEAALLTAWRALAGPRPRLLLVPRHP
                     QRFNDVAVLATQAGWSLTRRSQWSDGGPDDAALAAEVWLGDTMGEMPAYYA"
BASE COUNT          396 a          946 c          973 g          413 t
ORIGIN      
        1 cttcgacggc gacggcgacc gccttggcgt cgtgacgacc gacggccaga tcatctaccc
       61 cgatcgccag ctcatgctct tcgcccagga cgtgctgagc cgcgtgcccg gcggccagat
      121 catctatgac gtgaagtgca gccagcgcct ggccacggcg gtgcgcgagg ccggtggcaa
      181 gccgctgatg tggaagaccg ggcattcgct gatcaaggcc aagctcaagg agacgggcgc
      241 accactggcc ggcgagatga gcggccacat cttcttcgcc gaacgctggt acggcttcga
      301 cgacggcatg tacaccgccg cacgcctgct cgagatcttg agccgctatg acgacccgag
      361 cgcgctgctc aatgcgctgc cgaccagcca cagcacaccc gagctcaacg tggcctgcgc
      421 cgaaggcgag ccgcatgcgc tgatcgagaa attgcgcgcc gtggccagct tcgaaggggc
      481 gcgtgagctc gtgacgatag acggcctgcg tgccgactac gaagatggct tcggcctgat
      541 ccgcgcctcc aatacgacgc cggtgctggt gctgcgcttc gagggccaca cgccgcaggc
      601 gctggagcgc atccagggcc agttcatggc ggcgctgcat gcggtgaagc cggacgcgcg
      661 catcgccgcc gcggcgcatt gaagccgcta tcgccgcacc tggaagccac gcctggaacc
      721 gccttgcgcg tcctgatcgt caagctctcg tcgctgggcg acgtagtcca cgcgatgccg
      781 gtggtgcacg acattcggcg ggcccggccg ggtgcggcga tcgactgggt tgtggaaccg
      841 ggctttgcgc cgctggtgcg ccgcgtcgac ggcgtgcgcg aggtgatcga atgcgccgtg
      901 cggcgttggc gcaaggcctg gtggacgagc gccgtgcgca ccgagtggcg tgcctttcgc
      961 gagcggctgc agcgcgagcg ctacgacgcc gtgctcgatc tgcagggcct caccaagtct
     1021 gccctgatcg ccaggctggc gagcgtggac gccgtgggcc atcgctatgg tctgggcaat
     1081 cgcaccgacg gcgcgggctg ggagccgacg gcgcgctggc tcgtcgatcg cccgatattc
     1141 gtggcgccgc gcacccatgc gctggatcgc tcgcggctgc tcgcggcgca ggctttgggc
     1201 tatgaactcg aggcttcgcc ggtgttcggc ctgaacgcgc aggcgaagcc ttattcgccg
     1261 cctgtcgtgg ctttcgtgca cggcagctcg cgcgaagaca agctctggcc tgaggcgcat
     1321 tggatcgagc tcggccggcg cctgatcgac gaaagctatg gcattgcgct gccccagggc
     1381 agcgaagacg agcgcagacg cgccgagcgc atcgccgatg ctctcgggcc ggccgccaag
     1441 gtctggcccg gcatgggtct ggatacggtg gtcgattgcc tggcgggctg cacaggcgtg
     1501 atcggcgtcg acagcggcct gagccacatc gccgtggccc tggacctgcc ccatgtgcag
     1561 ctctacaact tcaagaccgc ctggcgcacc ggcccgcagc gctcgcatgg ccacgagcac
     1621 caggtctcca taggcggcga cgcgccacca gggctggagg aggtgtggca ggcctggctg
     1681 gaggtgcgtc ggcccggacg ggaggtcgac gagaccgagc gcgacaccca gccggccagc
     1741 tttacgccgt gaagccacct ccgtcccgac tgcgcagcag cctggcgcgg cgcgcctatt
     1801 caacgctgct gcaactgctc aagccctttt acgtggcgcg gctgtggtgg cgcgggcgcg
     1861 ccgaaccgct gtaccggcat cgcgtcgccg agcgcctcgg tgcctatcgc ggtgcgcgca
     1921 gcgaaggctg gatctggctg catgccgtct ccttgggcga gacgcgcgcc gccgccaccc
     1981 tgatcgccgc cttgcgcgag cgcaagcccg gcatgaagct gctgctcagc cacggcacgg
     2041 cgaccggccg cacggcgggg caggagctgc tggccgacgg cgaccagcag gtctggctgc
     2101 cctatgacac gccgggtgtg gtgcagcgct ttttctcgca gttccgtccc gcggtcggcg
     2161 tgctgatgga gaccgaggtc tggcccaatc tgctgcgcgg cgcgcagcgc gccggtgtgc
     2221 ccatgctgct ggccaatgca cgcctgagcg aacgcagcct cagtaaggcc ctgcggctcg
     2281 atgccgtgat gcgccccgcc gccgccagcc tgagcgccgt gctggcgcaa agcgaagccg
     2341 atgcccggcg cctcaagctg gccggcgcga ccagggtgca ggtctgtggc aatctcaagt
     2401 tcgacatcac gcccgacgcg ggcctgctgg cgcgcggcca gcgctggcgc gctgccagca
     2461 tccgccccat cgtgctggcc gcgaacacgc gcgagggcga ggaagcggcc ctgctgacgg
     2521 cctggcgcgc tctggctggg ccgcggcctc ggctgctgct ggtgccgcgc cacccgcagc
     2581 gcttcaacga cgtggccgtg ctggcgacgc aggccggctg gtcgctgacg cggcgctcgc
     2641 aatggtccga tggcggcccc gacgacgccg cccttgccgc cgaggtctgg ctgggcgaca
     2701 cgatgggcga gatgccggcc tattacgc
//
LOCUS       DASUCY010000228         3037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1530873, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000228 DASUCY010000000
VERSION     DASUCY010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1530873"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /gene="zapE"
                     /locus_tag="VFV25_04375"
     CDS             <1..312
                     /gene="zapE"
                     /locus_tag="VFV25_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AFG1/ZapE family ATPase"
                     /protein_id="HEY1392583.1"
                     /translation="LCGGPRSMNDYLELATQFHTVMLSDVPQMSPRLASEARRFTWLV
                     DVLYDRRVKLIISAAVPPEQLYTEGPLVHEFPRTVSRLHEMQSREYLAQARREVDTSL
                     T"
     gene            complement(340..1107)
                     /locus_tag="VFV25_04380"
     CDS             complement(340..1107)
                     /locus_tag="VFV25_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEY1392584.1"
                     /translation="MSTAPLGYRLNAATGIHRGDRMYQQDQVEILPHARVPGCLLAVV
                     ADGMGGKSGGRKAADQVLLTAKQLFERYAPSKDDPAEMLKQLVLEAHLMIKLTAITAE
                     EEPHSTVAAFLINPNRECNVIHAGDSRVYHFRGPDMLGRTIDHSFVQRLVEEGQITDE
                     EANSHPQANLLTGCLGTFQDPPLTLLRIAKLEVGDTLLACSDGLWHYFSPKELGAIVH
                     TLPPREASEMLIGKARQRARQGGDNLSLALVRVDLLT"
     gene            1178..1381
                     /locus_tag="VFV25_04385"
     CDS             1178..1381
                     /locus_tag="VFV25_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HEY1392585.1"
                     /translation="MDGNLHSPQRRLIELRIEHADLDSMIDRLAADRPLDELAMRRLK
                     KRRLLLRDQIARLEADLLPKEPA"
     gene            1378..>3037
                     /locus_tag="VFV25_04390"
     CDS             1378..>3037
                     /locus_tag="VFV25_04390"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEY1392586.1"
                     /translation="MSAGGSELQAAVAAAFSPGGALAAADARYREREVQQRFAQQVAQ
                     AIDAREVLVAEAGTGVGKTFAYLVPALLSGLRVLISTATKSLQDQLYLRDLPRLREAL
                     QVPVSVALLKGRSSYLCLHRLQQARQSAQLPDRFAVRALARIETWAQATRSGDLAELE
                     GLDERSPVIPLVTSTRENCLGSDCPEYRNCHVMKARREAMAADLIVINHHLFFADLAL
                     RDSGVAELLPSVDVAVFDEAHQLAEAGLQFLGATLGTGQLLDFARDMLGAGLQLARGL
                     APWNDLAADCDRAARDLRIAAAGALRDARGSIKLRWDERAQRADFQAALDAVARACAA
                     AEEALEGVDAAAPDFGKLAERATALAQRARAFGVEVEPGKVRWIDLSTHQARLVESPL
                     DVKAAMREQIARAPKAWVFTSATLGDDERLSWFTESAGLEEARCLRVGSPFDYPAHAR
                     LYVPLEFPKPNEPGHPLAVARLAARCAQALGGRSFVLTTTLRALQTIGDALSEALAAA
                     PGEPIQVLVQGRSPKRQLLQQFLASPRSVLVGSQSFWEGIDVPGD"
BASE COUNT          434 a         1054 c         1068 g          481 t
ORIGIN      
        1 ctgtgcggcg gcccgcgctc gatgaacgac tacctcgagc tcgccacgca gttccacacg
       61 gtgatgctgt cggacgtgcc gcagatgtcg ccgcggctgg cctccgaggc gcgccgcttc
      121 acctggctgg tcgacgtgct ctacgaccgc cgcgtcaagc tcatcatctc ggctgccgtg
      181 ccgcccgagc agctctatac cgaggggccg ctagtgcacg aattcccgcg caccgtgtcg
      241 cgcctgcacg agatgcagtc gcgcgagtac ctggcccagg cgcggcgcga ggtcgacacc
      301 agcctgacct agaccgcgct accgaaaggc tcagcccttc taggtcagga gatcgacccg
      361 caccagcgcc agcgagaggt tgtcgccgcc ctggcgcgcg cgctggcgcg ccttgccgat
      421 cagcatctcg ctggcttcgc gcggcggcag cgtgtgcacg atggcaccca gctccttggg
      481 gctgaagtag tgccacaggc cgtcgctaca ggcgagcagg gtgtcgccga cctccagctt
      541 ggcgatgcgt agcagggtca gcggcgggtc ctggaaggtg cccaggcagc cggtcagcag
      601 gttggcctgc ggatggctgt tggcctcttc gtcggtgatc tggccttcct cgaccaggcg
      661 ctgcacgaag gagtggtcga tggtgcggcc cagcatgtcg gggccgcgga agtgatagac
      721 gcgcgaatcg ccggcgtgga tcacgttgca ttcgcgattc gggttgatca ggaaggccgc
      781 gaccgtgctg tgcggctcct cctcggccgt gatcgccgtg agcttgatca tcagatgcgc
      841 ttccagcacc agctgcttga gcatctcggc cggatcgtcc ttggacggag cgtagcgctc
      901 gaacagctgc ttggcggtga gcagcacctg gtcggcggcc ttgcgcccgc cgctcttgcc
      961 gcccatgccg tcggccacga cggccagcag gcagccgggc accctcgcgt ggggcaggat
     1021 ctcgacctgg tcctgctggt acatgcggtc gccacgatgg atgccggtgg cggcgttgag
     1081 gcgataaccc aggggtgctg tactcatgag cgaaaactat aatcgctttg ggttctcccc
     1141 gagcttgcaa cttcctacat ttgtccgcgt gcgcgcaatg gatggcaacc tccactctcc
     1201 tcagcgccgc ctgatcgaat tgcgcatcga acacgccgat ctcgacagca tgatcgaccg
     1261 gcttgccgcc gatcggccgc tggacgagct cgccatgcgc cgactcaaga agcgccgctt
     1321 gctgctgcgc gaccagatag cccgcctgga agccgatctg ttgccgaaag agccggcgtg
     1381 agcgcgggcg gcagcgagct gcaggccgcg gtcgcggcgg ccttttcgcc cggtggcgcg
     1441 ctggcggcgg ccgatgcccg ctaccgtgag cgggaggtgc agcaacgctt cgcccagcag
     1501 gtggcgcagg ccatagacgc gcgcgaagtg ctagtggccg aggccggcac cggcgtcggc
     1561 aagaccttcg cctacctggt tccggctttg ctgtcgggcc tgcgcgtgct gatcagcacg
     1621 gccaccaaga gcctgcagga ccagctctac ctgcgcgacc tgccgcgcct gcgcgaggcc
     1681 ctgcaggtgc cggtcagcgt ggccctgctc aaggggcgct ccagctatct ctgtctgcac
     1741 cggctgcagc aggcgcggca gtcggcgcaa ctgcccgatc gcttcgcggt gcgcgcgctg
     1801 gcgcgcatcg agacctgggc gcaggccacc aggagcggtg acctggccga gctcgagggc
     1861 cttgacgaac gctcgccggt gataccgctg gttacgtcga cgcgcgagaa ctgcctgggc
     1921 tcggattgcc ccgagtaccg caactgccat gtgatgaagg ccaggcgcga ggccatggcg
     1981 gccgacctga tcgtcatcaa ccaccatctc ttcttcgccg atctggccct gcgcgacagc
     2041 ggcgtggccg agctgctgcc cagcgtcgac gtggccgtct tcgacgaagc gcatcagctg
     2101 gccgaggcag gcctgcagtt tctgggcgcg accctgggca ccggccagct cctcgacttc
     2161 gcgcgcgaca tgctgggcgc cggcctgcag ctggcgcgcg ggctggcgcc ttggaacgac
     2221 ctggccgccg attgcgatcg cgcggcacgc gatctgcgca tcgcggccgc cggagccctg
     2281 cgcgatgcgc ggggcagcat caagctgcgc tgggacgagc gcgcgcagcg tgctgatttt
     2341 caagccgcgc tggatgccgt ggcgcgggcc tgcgcggcgg ccgaggaagc gctggagggc
     2401 gtggacgcag cggcgcccga tttcggcaag ctggccgagc gcgcgaccgc cctggcccag
     2461 cgcgcgcggg ccttcggcgt cgaagtggag ccgggcaagg tccgctggat cgacctcagc
     2521 acgcatcagg cccgcctggt cgagtcgccg ctggacgtga aggccgcgat gcgcgagcag
     2581 atcgcccgcg cgcccaaggc ctgggtcttc acctcggcca cgctgggcga cgacgagcgc
     2641 ctgagctggt tcaccgagtc cgccggcctg gaggaggcgc ggtgcctgcg cgtcggcagc
     2701 cccttcgact atccggcgca cgcacgcctg tacgtgccgc tcgagtttcc caagcccaac
     2761 gagcccggcc atcccttggc cgtggcgcgg ctcgcggcgc gctgcgcgca ggccctgggc
     2821 ggccgcagct tcgtgctgac gaccaccttg cgcgcactgc agacgatagg cgatgcgctg
     2881 agcgaggccc tggccgcggc gccgggcgag cccatccagg tgctggtgca gggccgctcg
     2941 cccaagcgcc agctgctgca gcaattcctg gcctcgccgc gcagcgtgct ggtcggctcg
     3001 cagagcttct gggagggcat agacgtgccc ggcgacg
//
LOCUS       DASUCY010000229         6272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1097142, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000229 DASUCY010000000
VERSION     DASUCY010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 6272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6272
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097142"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /gene="selD"
                     /locus_tag="VFV25_04395"
     CDS             <1..507
                     /gene="selD"
                     /locus_tag="VFV25_04395"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007839232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEY1392587.1"
                     /translation="VGVLSAALKKGKLDAAGYAQLIQTTTRLNKPGTALAELSGVHAI
                     TDITGFGLAGHTLELARGAGLKAVVEWPRLPLLPGVASMAADGFVTGASGRNWAGYGT
                     EVRLDPALPAVARDLLTDPQTSGGLLVSCSPEAADAVLGIFRAQGFGEAVMIGRVEAG
                     TPGLEVLA"
     gene            complement(509..2968)
                     /locus_tag="VFV25_04400"
     CDS             complement(509..2968)
                     /locus_tag="VFV25_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEY1392588.1"
                     /translation="MASGPDPLPEDVGWDALPGAVILLGPDAGAVYVNRAYCELTGMS
                     ADAALGQGWLQALEPDSRSRVLAGARSRGDFSLQLAMHRQRAAGRGKGAEVGVTSDDE
                     LWLSAAGRWLPQRGQHVCVLHDVSTAKLAERAMLMQAEQFQLLANSVPVLIAYYDAPE
                     AGGRCRFANRQYAATFGLTEASIIGKTFPEIIGEEAAREVQPYIDEVVEKSHAVAYVR
                     QLDGAEGTRRWIEVNVMPHLGSHGRPVGAFVVVTDITQHRLAAEAVRASEERLAKFMH
                     ASAEGIVFHKDGIIIDANPPLCTMLGYTLEEVRGRKSLEFVAPDQTSRVAAVIMAGKD
                     ITYESVLLHKDGRRIPVELIGRTMVFENEKLRMAVVRDLRDRHAAQARIHYLAHHDPL
                     TGLPNRLSFMEQLEHQIALARRANTQLALLFVDLDHFKRVNDSLGHLVGDALLQTVAA
                     RISACLRTTDQVARFGGDEFVVLLSGVAHRSDVVEVAGKLLAAIEVPVDAQGRNISVT
                     PSVGIAMFPEHGQTPTELIKHADTAMYLAKSRGRANHQFFDPAAANAAYAALELESQL
                     AEALEHNEFLLHFQPQVSAQDGALAGIEALIRWKHPQRGLLMPDEFIPLAEERRLMLP
                     IGQWVMQAASRCAKRWRSAGLIQVPVAINLSSMQFHADGFVEAVEQALREEQLPGSWI
                     ELELTERMLMDDLGAVKRTLKRLKSLGIRISVDDFGTGYSSLAHLKELPIDRIKIDRS
                     FVQDLPRDQGSAAIARAIVQMAKGLGLTAIAEGVETKAQRRFLADEGCDELQGELISA
                     PLDEAGLEAWAVKRILAEPAS"
     gene            complement(3104..3907)
                     /locus_tag="VFV25_04405"
     CDS             complement(3104..3907)
                     /locus_tag="VFV25_04405"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY1392589.1"
                     /translation="MSWSERQIAMLEEMGIRLWRQPQPDTLQDAGSLPPSERGEGAAL
                     AAPPLSARTIQPLAAPQDAVLRGPRPTGVEQMDWRALREAVAGCTACKLCEGRRNTVF
                     GVGNENAHWMLIGEAPGEQEDRQGEPFVGKAGQLLDNMLAAVGLTRSDAPPERQVFIA
                     NVLKCRPPMNRNPELDEIAQCEPFLKRQVELVAPRLILAMGRFAVQSLLQTTEPIGRL
                     RGRVHRYQGVPVVVTYHPAYLLRNPQDKARSWEDLCLAMELMRGSAARD"
     gene            complement(3894..4403)
                     /gene="rimI"
                     /locus_tag="VFV25_04410"
     CDS             complement(3894..4403)
                     /gene="rimI"
                     /locus_tag="VFV25_04410"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEY1392590.1"
                     /translation="MSAQLRPERWLQDMRVDDLDEVLRVEQLAYSHPWSRGNFVDSLH
                     AGYLAQTLRADDGALLGYFIAMPGVDEVHLLNLTVAPGEQGRGHARFMLDMLSLAARA
                     EHHAAKLWLEVRVSNARALALYERYGFRRAGLRRGYYPLKPQQREDALVMSLDLMQIP
                     QKGACDELV"
     gene            complement(4512..5225)
                     /gene="tsaB"
                     /locus_tag="VFV25_04415"
     CDS             complement(4512..5225)
                     /gene="tsaB"
                     /locus_tag="VFV25_04415"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEY1392591.1"
                     /translation="MSAPRLLVLDTATEVMHLGLLLGEELHSVEAEGGARASAELLPG
                     ILKLLADAGIALRELDAIAFGQGPGAFTGLRTACSVAQGLAYGAGRPVIALDTLLAVA
                     EHARIQGAGPQLWALNDARMGEIYAARYAYSAGRWQTLAAAALYSPAALMERLRDEAP
                     EVALAGNALSAHAEALSGMRRPCWPEARPCAAALAALARAAWARDDILPAEQALPVYV
                     RDKVAQTTAEREAARGVAS"
     gene            5274..>6272
                     /locus_tag="VFV25_04420"
     CDS             5274..>6272
                     /locus_tag="VFV25_04420"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEY1392592.1"
                     /translation="MLSVLCRAGLYLLLAVSSALACAAGLPPAVAKALKQARLPQDAL
                     VAVVAEAGRPERPRLSYQAELPVNPASLMKLFTTYAALDLLGPAYSWTTPVYLDGRVV
                     NPGPVGVLEGSVYIQGRGDPTLVLERVWLLVQRLRQAGVREIRGDIVLDRSAFSVPDA
                     DPGDFDGEPYRPYNVRPDALLFNFKSLTLSFEPDASAGVARISVEPTLAGLRIDESVA
                     LANGPCEDWRSALKADFNDPARIRFGGGFAATCGERQWPLAYADPRSYNARLLIALWQ
                     GAGGLLSGQVREGTVPPGLKPAFEFGSPPLADAVRGINKFSNNTMAQQLFLTLGLTQ"
BASE COUNT          981 a         2176 c         2127 g          988 t
ORIGIN      
        1 gtgggcgtgc tgtcggcggc gctgaagaag ggcaagctcg atgcagccgg ctatgcgcag
       61 ttgatccaga ccacgacccg gctgaacaag ccgggcacgg cgctcgccga gctgagcggc
      121 gtgcatgcga tcaccgatat cacgggattc ggcctggccg gacacaccct ggaactggca
      181 cgtggggcgg gcctcaaggc cgtggtcgaa tggcccaggc tgccgctgct gcccggcgtg
      241 gcgagcatgg cggccgatgg cttcgtgacc ggcgcctcgg gccgcaactg ggccggctac
      301 ggcaccgaag tccggctcga tccggccttg cccgccgtag cgcgcgatct gctcaccgac
      361 ccccagacct cgggcggcct gctggtgagc tgcagcccgg aagcagccga tgcggtgctg
      421 ggcatcttcc gcgcccaggg cttcggcgag gccgtaatga tcggccgcgt ggaagccggc
      481 acgccggggc tcgaagtcct ggcctgattt acgaggccgg ctccgcaagg atgcgcttga
      541 cggcccaggc ttccagccca gcctcgtcga gcggcgcgct gatcagctcg ccttgcagct
      601 cgtcgcagcc ctcgtcggcc aggaagcggc gctgcgcctt ggtctcgacc ccctcggcga
      661 tcgccgtcaa gcccaggccc ttggccatct gcacgatggc acgcgcaatg gcggccgaac
      721 cctgatcgcg cggcaagtcc tgcacgaagg agcggtcgat ctttatgcgg tcgatcggca
      781 gctccttcag gtgtgcgagc gaggaatagc cggtgccgaa atcgtcgacc gagatgcgta
      841 tgcccaggct cttgagccgc ttgagcgtgc gcttcacggc gccgaggtcg tccatcagca
      901 tgcgctcggt gagctcgagc tcgatccagc tgccgggcaa ttgctcctcg cgcagcgcct
      961 gctcgacggc ctcgacgaag ccgtcggcat gaaattgcat gctggacaga ttgatcgcca
     1021 ccggcacctg gatcagccct gcgctgcgcc agcgcttggc gcagcgcgag gcggcctgca
     1081 tcacccattg cccgatcggc agcatcagcc gccgctcctc ggccaggggg atgaactcgt
     1141 cgggcatcag caggccgcgc tgcggatgct tccagcgtat cagcgcttct atgcccgcca
     1201 gggccccgtc ctgcgcgctc acctggggct ggaagtgcag caggaactcg ttgtgctcca
     1261 gcgcctcggc gagctggctc tccagctcca gggccgcata ggcggcattg gccgcggcag
     1321 ggtcgaagaa ctggtgattg gcacgcccgc gtgacttggc gaggtacatc gctgtgtcgg
     1381 catgcttgat gagctcggtc ggcgtctggc catgctcggg aaacatcgcg atgcccaccg
     1441 aaggcgtcac cgagatgttg cggccctggg cgtccaccgg cacctcgatc gccgccagca
     1501 gcttgcccgc cacttccacc acgtcgctgc gatgggccac gccgctgagc aggaccacga
     1561 actcgtcgcc gccgaagcgc gccacctggt cggtggtgcg caggcaggcc gagatccgcg
     1621 ccgccacggt ctgcagcaag gcatcgccga ccagatgccc cagcgaatcg ttgacgcgct
     1681 tgaaatggtc gagatcgacg aaaagcagcg cgagctgggt gttggcgcgc cgcgccagcg
     1741 cgatctgatg ctcgagctgc tccatgaagg acagccggtt gggcaggcct gtcagcgggt
     1801 cgtggtgggc gagatagtgg atgcgcgcct gggcggcatg gcggtcgcgc aggtcgcgca
     1861 ccacggccat gcgcagcttc tcgttctcga acaccatggt ccgcccgatc agctcgaccg
     1921 ggatgcgcct gccgtccttg tgcagcagca cgctctcgta ggtgatgtcc ttgccggcca
     1981 tgatcaccgc ggcgacgcgg ctggtctgat cgggcgcgac gaactcgagc gacttgcggc
     2041 cgcgcacctc ctccagcgtg tagccgagca tcgtgcacag cggcgggttg gcatcgatga
     2101 tgatgccgtc cttgtggaag acgatgcctt cggcactggc atgcatgaac ttggccagcc
     2161 gctcctcgga ggcgcgcacc gcctccgcgg caaggcggtg ctgggtgatg tcggtcacga
     2221 cgacgaaggc gccgaccggc ctgccgtggc tgcccaggtg gggcatcaca ttgacctcga
     2281 tccagcgcct ggtgccctcc gcaccgtcga gctggcgcac ataggccacc gcgtggctct
     2341 tctcgaccac ttcgtcgata tagggctgga cctcgcgcgc cgcctcttcg ccgatgatct
     2401 cgggaaaggt cttgccgatg atgctcgctt cagtcagacc gaaggtggcg gcgtactgcc
     2461 ggttggcgaa gcggcagcgc ccgcccgcct cgggcgcgtc gtagtaggcg atcagcaccg
     2521 gcacgctgtt ggcaagcagc tggaactgct cggcctgcat cagcatggcg cgctcggcca
     2581 gctttgcggt ggaaacgtcg tgcagcacgc agacatgctg gccgcgctgc ggcagccagc
     2641 gcccggcagc gctgagccac agctcatcgt cgctggtcac gccgacctcg gcgcccttgc
     2701 cgcggccggc agcgcgctgg cgatgcatcg ccagctgcag ggagaagtcg ccacggctgc
     2761 gcgcccctgc caacacgcgg gagcgcgagt cgggctccag agcttgcagc cagccctggc
     2821 cgagcgcggc gtcggcgctc atgcctgtca gctcgcaata ggcgcggttc acatagacgg
     2881 cgcccgcgtc cgggccgagc aatatcaccg cgccgggcag cgcgtcccag ccgacatcct
     2941 cgggtagtgg atcagggcct gaggccatgg gcgagactcc gaatcggtcg gtgcgaccga
     3001 aggattcgat gatagtgagc cgccgctcgt cgcggcatcc ctacaagggg ggagaggcaa
     3061 gatccgtgtg actttgggcc agaaaccact actgcgagtg cgttcagtcg cgcgctgccg
     3121 agcctcgcat cagctccatc gcgaggcaca agtcctccca ggatcgggcc ttgtcctgcg
     3181 ggttgcgcaa cagataggcg gggtgatagg tgacgaccac gggaacgccc tggtaacgat
     3241 gcacgcggcc gcgcagccga ccgatcggct cggtggtctg cagcagcgac tgcacggcga
     3301 agcgccccat cgccaggatc agcctgggcg ccaccagctc gacctggcgc ttgaggaagg
     3361 gctcgcattg cgcgatctcg tcgagctcgg gattgcgatt catcggcggg cggcacttca
     3421 gcacattggc gatgaagacc tgccgctcgg gcggagcgtc agagcgggtc aggcccaccg
     3481 cggccagcat gttgtcgagc agctgcccag ccttgcccac gaagggctcg ccctgcctgt
     3541 cctcctgctc gccgggcgcc tcgccgatca gcatccagtg cgcgttttcg ttgccgacgc
     3601 cgaagaccgt gttgcggcgc ccctcgcaga gcttgcaggc cgtgcagccg gcgacggctt
     3661 cgcgcagggc ccgccagtcc atttgttcga caccggtcgg acgcggcccg cgcaataccg
     3721 catcttgcgg cgctgcaagg ggttgtatgg tccgggcgga gagcggcggc gcggccaatg
     3781 ccgcgccctc ccccctctcg gaggggggca gggagccggc gtcctgcagg gtatccggtt
     3841 gtggctggcg ccacagccgg atacccatct cctcgagcat cgcgatctgg cgctcagacc
     3901 agctcatcgc aggctccctt ctgcgggatc tgcatcagat ccaggctcat gaccagcgcg
     3961 tcttctcgct gctgcggctt cagcgggtaa tagccgcggc gcaagccggc gcggcgaaag
     4021 ccgtagcgct catagagcgc caaggcgcgc gcattgctca cacgcacttc cagccacagc
     4081 ttggcggcgt gatgctccgc acgggccgcc aggctcagca tgtccagcat gaagcgggca
     4141 tggccgcgcc cttgctcgcc gggcgccacc gtgagattga gcagatgtac ttcatcgacg
     4201 ccgggcatcg cgatgaaata gcccagcagc gcaccgtcgt cagcacgcag ggtctgcgcg
     4261 aggtagccgg catgcagcga atcgacgaag ttgccgcggc tccaggggtg gctgtaggcc
     4321 agctgctcga cacgcaagac ctcatcgaga tcgtcgaccc gcatgtcctg cagccaacgc
     4381 tcagggcgca gttgggcact catgatgacg gcccctcgtc cgacgagtac atcagccggt
     4441 cccccgaggg gacgcgggcc ggcttgggag cggcccggcg ctcggcccgc gaacagattg
     4501 acgccgatgt ctcatgacgc aactccgcgt gccgcttcgc gctcagccgt ggtctgcgcc
     4561 accttgtcac gcacatagac cggcaaggcc tgctcggccg gcaagatgtc gtcgcgcgcc
     4621 catgcggcac gcgccagcgc ggcaagggct gccgcgcaag gccgcgcctc gggccagcac
     4681 gggcggcgca tgccgctcag ggcctcggca tgcgcgctca gggcattgcc cgccagcgcc
     4741 acctcgggtg cttcgtcgcg caggcgttcc atcaaggccg caggactgta gagcgccgcc
     4801 gcagccaggg tctgccagcg ccccgccgaa taggcatagc gggcggcata gatctcaccc
     4861 atgcgcgcgt cgttgagggc ccacagctgc ggccctgcgc cttggatgcg cgcatgctcg
     4921 gcaacagcga gcagggtgtc gagggcgatc accggccgcc cggcaccata ggccaggccc
     4981 tgggctactg aacaggctgt acgcaagccg gtgaaagcgc ccggcccctg cccgaaggcg
     5041 atcgcatcga gctcgcgcag cgcgattccc gcgtccgcca gcagcttcaa aatgccgggc
     5101 agcaattcgg cggaggcccg tgccccgcct tcggcttcga cgctgtggag ttcctcgccc
     5161 agcagcaggc cgagatgcat gacctcggtg gcggtatcca gcacaagcag acggggggcg
     5221 ctcatgccgg cagtttagcc agcgcccgcg aagccgcccc gtatcatcgg cccatgcttt
     5281 cagtgttatg ccgcgccggc ctgtatctgc tgctggcggt ttcatccgcc ctggcctgcg
     5341 cggccggcct gccgcctgcc gtggcgaagg cgctcaaaca ggccaggctg ccgcaggacg
     5401 ctttggtggc cgtggtggcc gaagcaggca ggcccgagag accgcgcctg tcctaccagg
     5461 cagaactgcc ggtgaacccg gcttccttga tgaagctgtt caccacttac gccgcgctgg
     5521 acctgctcgg ccccgcctac agctggacga cgcccgtgta tctggacggc cgcgtcgtca
     5581 accccgggcc tgttggcgtg ctcgaaggct cggtctacat ccagggccgc ggcgatccca
     5641 cgctggtact ggagcgggtc tggctgctgg tgcagcggct gcgccaggcc ggcgtgcgcg
     5701 agatccgagg cgatatcgtg ctcgatcgca gcgccttcag cgtgcccgac gccgatcccg
     5761 gcgacttcga cggcgagcct tatcggccct acaacgtgcg gcccgatgcg ctgctgttca
     5821 acttcaagtc gctgaccctg agcttcgagc ccgatgcctc ggcaggcgtc gcgcggatca
     5881 gcgtcgaacc cacgctggcc ggcctgcgca tcgacgaaag cgtcgcgctc gccaacggcc
     5941 cttgcgagga ctggcgcagc gcgctcaagg ccgacttcaa cgacccggca cgcatacgct
     6001 tcggcggcgg ctttgccgcg acctgcggcg agaggcaatg gccgctcgcc tatgccgatc
     6061 cacgcagcta caacgcgagg ctgctgatcg ccctgtggca gggcgcgggc ggcctgctga
     6121 gcggccaggt gcgcgaaggc acggtgccgc ccggcctgaa gcccgccttc gaattcggtt
     6181 cgccgccgct ggccgatgcg gtgcgcggca tcaacaagtt cagcaacaac accatggccc
     6241 agcagctgtt cctcactctg ggcctcacgc aa
//
LOCUS       DASUCY010000230         2684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1546976, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000230 DASUCY010000000
VERSION     DASUCY010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1546976"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="VFV25_04425"
     CDS             complement(<1..658)
                     /locus_tag="VFV25_04425"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEY1392593.1"
                     /translation="MNTPRIAVTGIGLVSPLGQDKHQAWRRLMDGEGGIEALGGEGHQ
                     PTIPAARLGDFQASRWLNKLQMVGTERVSQMALGAARLAMDDAGLGAAFGDPERAGLY
                     LGTGMGGAATVDAGYAALHAGRHLPPLTVPTGMVNNAAAIVAMHAHVGGPVLTFSVAC
                     ASSAIAIAEAAHALRRGEIDVALAGGSEALLVRGSIAAWRALHTLAPLHASDVRDSCR
                     P"
     gene            complement(655..915)
                     /locus_tag="VFV25_04430"
     CDS             complement(655..915)
                     /locus_tag="VFV25_04430"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEY1392594.1"
                     /translation="MDHTIEQLKQLIHEQFDIAVDSIDADAPFASYNLDSLTVAELVF
                     AIEDQFHVDFPNDLSQIQTLRDLAGVLDALPRREADTSPSTA"
     gene            complement(930..1712)
                     /locus_tag="VFV25_04435"
     CDS             complement(930..1712)
                     /locus_tag="VFV25_04435"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY1392595.1"
                     /translation="MLLHGLLSSPKEFGLVHHPLRRAGVKMIAPELQGYTLGTGEPGR
                     QWQDWLLAASAALDELPGHEPVVLGGLCTGAMLATALALARPQRVRGLVLLSPLLAYD
                     GWGLPWWYRLRRIAYVLHIESLFAMREREPFGLKNEKMRLWVRQQLQTQGDSVAGPAR
                     IPLWAVRQSELLSAHVSARLKDLAVPVRTVHAREDEICSLASVERVLRAIDPARHQMS
                     VLDNSYHMITADNDRNLLVHQLQAFVHGLGGAESAASARVRA"
     gene            complement(2095..2622)
                     /locus_tag="VFV25_04440"
     CDS             complement(2095..2622)
                     /locus_tag="VFV25_04440"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY1392596.1"
                     /translation="MSEQETKKSPVLSSVNADGIYEPFEIGHVPWVEFSHGERYGLRY
                     QHLSSFGGGSQISVSMEILPPGKQANQAHFHLLEEEHVFVLEGALTLRLGDKSYVMSA
                     GHYVCFPAGQKLGHALINHTAEPCRYLVLGNPHPHDVAVHTDTGRVSVKLTGEGYRGS
                     ATMGYWDGADAGGVS"
BASE COUNT          440 a          895 c          920 g          429 t
ORIGIN      
        1 aggggcggca gctgtcccgc acatcgctcg catgcagcgg cgccagcgta tgcagggccc
       61 gccaggcggc gatgctgccg cgcaccagca gggcctcgct gccgccggcc agcgcgacgt
      121 cgatctcgcc gcggcgcagc gcatgcgcgg cctcggcgat cgcgatggcg gaagacgcgc
      181 aggctactga aaacgtcagc accggcccgc cgacatgcgc atgcatggca acgatggccg
      241 cagcgttgtt gaccatgccg gtgggcacgg tcagcggcgg cagatgccgg ccggcgtgca
      301 acgcggcata gcccgcgtcc accgtcgcgg cgccgcccat gccggtgcct aggtaaagtc
      361 cggcgcgctc ggggtcgccg aaggcagcgc ccaagccggc atcgtccatt gccaggcggg
      421 ccgcgcccag ggccatctgg ctgacgcgct cggtgccgac catttgcagc ttgttgagcc
      481 agcgcgaggc ttggaagtcg cccaagcggg cggcggggat ggtgggctgg tggccctcgc
      541 cgcccagggc ctcgatgccg ccttcgccgt ccatcaggcg gcgccaggcc tgatgcttgt
      601 cctggcccag cgggctgacg agaccgatgc cggtgacggc gatgcgcggc gtgttcacgc
      661 ggtggaaggg ctggtatccg cttcccggcg cggcagcgcg tccagcacgc ctgccaggtc
      721 acgcaaggtc tggatctgcg acaggtcgtt ggggaaatcg acgtggaact ggtcctcgat
      781 ggcgaagacc agctcggcca ccgtcagcga atcgaggttg taggacgcga agggcgcgtc
      841 ggcatcgatg ctgtcgacgg cgatgtcgaa ctgctcgtgg atcagctgct tgagttgttc
      901 gatcgtatgg tccatgaggg agagactgct taggcacgca cccgggccga cgcggcggat
      961 tcggctccgc ccaggccgtg cacgaaggcc tgcagctggt gcaccagcag gttcctgtcg
     1021 ttgtccgccg tgatcatgtg atagctgttg tccagcacgc tcatctggtg gcgcgccggg
     1081 tctatggccc gcagcacgcg ctcgacgctg gccagcgagc agatctcgtc ctcgcgcgcg
     1141 tgcacggtgc gcaccggcac cgccaggtct ttcaggcgcg cgctcacgtg ggccgacagc
     1201 agctcgctct ggcgcaccgc ccacaaggga atgcgggcgg ggccggccac ggaatcgccc
     1261 tgggtctgca gctgctggcg cacccacagg cgcatcttct cgttcttgag gccaaagggc
     1321 tcgcgctcgc gcatcgcgaa tagcgactcg atgtgcagca cgtaggcgat gcggcgcagt
     1381 cggtaccacc agggcaggcc ccagccgtcg taggcgagca gcggcgacag caacaccagg
     1441 ccccgcacgc gctgcggccg cgccagcgcc agcgcggtcg ccagcatggc gccggtgcac
     1501 agtccgccca acaccaccgg ctcatggccg ggcagctcgt ccagggcggc cgaggctgcg
     1561 agcagccagt cctgccattg ccggcccggc tcgccggtgc cgagcgtata gccctggagc
     1621 tccggggcga tcatcttcac gcccgcgcga cgcagcgggt ggtggaccag gccgaattcc
     1681 ttgggcgagc tcagcaggcc gtgcaacagc accagccgtg gcgtatggcg gtgagaggaa
     1741 agctgatgat gagtcacgaa atggagattg agtaagcgcc ggctcaggcc tcaaagaggc
     1801 agcctttgat tatgcagccc tgtaacaacc gccctgttgt cggcaaaccc gccttgaaag
     1861 tcggcagcgc gggcccggaa gccttggttt gcctcgatgg atccaccgaa cgcggcaaaa
     1921 atggaccggc gaatatggcc tgagcgcctc cagcggcttg ttgaggcgct taccttttga
     1981 agtctttcag gcctctcggg tgttgcgaat tgtcccagaa gcggaattcc ccagaaagcc
     2041 gccgccttta aggaagggac cgcgccgcgg cggccagcac agcgacgagc cgcttcagga
     2101 cacgcctccc gcgtccgcgc catcccagta gcccatggtc gccgagccgc ggtagccctc
     2161 gccggtcagc ttgacgctca cccggccggt gtcggtgtgc accgcgacat catgaggatg
     2221 cgggttgccg agcactaggt agcggcaggg ctcggccgta tggttgatca gcgcatggcc
     2281 cagcttctgc ccggcgggaa agcacacata gtgtccggcc gacatcacat agctcttgtc
     2341 gcccagccgc agggtgagcg ccccttccag cacgaagaca tgttcctctt cgagcaggtg
     2401 gaaatgcgcc tgattggcct gcttgcccgg cggcaggatc tccatgctca cgctgatctg
     2461 cgacccgccg ccgaagctgc tcaggtgctg gtagcgcagc ccatagcgct cgccgtgcga
     2521 gaactcgacc cagggcacgt ggccgatctc aaagggttcg taaatgccat ctgcgttgac
     2581 cgaggacagc accggcgact tcttcgtttc ttgttcgctc atgctcaatt gcgctcatgg
     2641 tggtctaggg cttgccggcc agcaaggcgg cgcggcgctc cagg
//
LOCUS       DASUCY010000231         2119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_840076, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000231 DASUCY010000000
VERSION     DASUCY010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840076"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="VFV25_04445"
     CDS             complement(<1..1064)
                     /locus_tag="VFV25_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEY1392597.1"
                     /translation="MQFTPSQKRGLIWAAIALAAAALLWLLAPVLTPFALAAVAAYAL
                     HPAVERLAARRVPRALAVLLVEVVAIVLALGLLLLIVPILSRELPLLRDKIPVLATHL
                     NDTLAPWLAQLGVDVALDPASVKAFVLKHLNANYEEWLASALSSARLGGSYVLAVVGN
                     AVLVPLVLFYLLMDWPRLAAHLENLVPPQRRHGVYAFAKECDDMLGQYLRGQLLVMVI
                     LAVYYSVGLALFRFDLAVPVGVFTGLAVCIPYVGFGLGLLLALLAGLLQYASVYGFVA
                     VAVIYGLGQVLEGFFLTPRLVGERIGLHPIVVIFALLAFAHLFGFVGVLVALPVSAIG
                     MVALRRLKALYLGSRLYLGEP"
     gene            complement(1313..>2119)
                     /locus_tag="VFV25_04450"
     CDS             complement(1313..>2119)
                     /locus_tag="VFV25_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEY1392598.1"
                     /translation="DASLALTEAAIALAQDEFPGLDTQGVLTEIDALADKLRRRLAPD
                     AGALQKLRLLNRYFFDELGFAGNVNDYYDPHNSYLHRVLATRRGIPVSLAVLYMELAS
                     QIGLSARGVGFPGHFLVKLKLHAAGEVMMDPFSGLSLSREQLDERLAPYRRRQARSGE
                     EELPLAIFLQPATPRDVLARMLRNLKEIHRSTGNWPALLGVLQRLVVLLPDDMGERRD
                     RGLVLAELGRYSEAAQDLDAYLARRPSAPDGAALKLRAEQLRAQGRPRLH"
BASE COUNT          405 a          717 c          723 g          274 t
ORIGIN      
        1 ggctcaccga gatagagccg gctgccgaga tacagcgctt tcaagcggcg cagcgccacc
       61 atgccgatgg cgctgacggg cagcgcgacc agcacgccaa cgaagccgaa cagatgcgcg
      121 aaggcgagca gcgcgaagat cacgacgatg ggatgcaggc cgatgcgctc gcccaccagg
      181 cgcggcgtga ggaagaagcc ttcgagcacc tggcccaggc cgtagatcac ggccacggcg
      241 acgaagccgt agacgctggc gtattgcagc aggccggcca gcagcgccag cagcaggccc
      301 aggccaaagc ccacataagg gatgcacacc gccaggccgg tgaacacgcc caccggcacg
      361 gccaggtcga agcggaacag cgccaggcct acgctgtagt agacggccag gatcaccatc
      421 accagcagct ggccgcgcag atactggccc agcatgtcgt cgcattcctt tgcgaaggca
      481 tagacgccat ggcgccgctg cggcggcacc aggttctcga gatgcgccgc cagccgcggc
      541 cagtccatca gcagatagaa cagcaccagc ggcaccagca ccgcattgcc gaccacggcc
      601 agcacatagc tgccgcccag gcgggccgag ctgagcgcgc tggccagcca ttcctcgtag
      661 ttggcgttga ggtgcttgag cacgaaggcc ttgacgctgg ccgggtccag cgcaacatcg
      721 acgcccagct gggccagcca gggcgcgagc gtgtcgttga gatgcgtggc cagcacggga
      781 atcttgtcgc gcagcagcgg cagctccctg ctcaggatgg gcacaatcag cagcagcagg
      841 cccagggcca gcacgatggc aaccacctcg accagcagca ccgccagcgc gcgcggcacg
      901 cgccgcgccg ccaagcgctc cacggccggg tgcagggcat aggcggccac cgcggcgagc
      961 gcgaagggcg tcagcaccgg cgccagcagc cacagcagcg ccgccgccgc cagcgcaatc
     1021 gccgcccaga tcaggccgcg cttttgtgag ggcgtgaatt gcatgagagg gtggggaagg
     1081 cgtaatgaag gactggcccg aggcggacgg cgcgaagccg cgcggattct aggggcggcg
     1141 ccccgccggg acggagcctc gagccctggt tcaagcggca acccggccgt tcaaaccttg
     1201 aatattttgt cccaaaagga tagtagccgg ctattcgagg accaggaccc tttgtatttc
     1261 gctagcagtg gacgctcagc aaatctagaa aaatgtcccg atgtgacagt cttcaatgca
     1321 gccgcggccg cccctgggcg cgcaattgtt ccgcgcgcag cttcaaggcc gcgccgtccg
     1381 gagcggatgg ccgccgcgcg agataggcat ccaggtcttg cgccgcctcc gaatagcgcc
     1441 cgagctcggc cagcacgagg ccacggtcgc ggcgctcgcc catgtcgtcg gggagcagaa
     1501 ccacgaggcg ctgcagcacc ccgagcagag ccggccagtt gccggtactg cggtggatct
     1561 ccttgagatt gcgcagcatg cgggccagca cgtcgcgcgg cgtggctggc tgcaggaaga
     1621 tggccagcgg caattcctcc tcgcccgagc gagcctgccg gcgccggtag ggcgcaagcc
     1681 gctcgtccag ttgctcgcgc gacagcgaca gcccgctgaa gggatccatc atcacttcgc
     1741 ccgcggcgtg cagcttgagc ttgacgagga aatgcccggg aaagcccacg ccgcgcgccg
     1801 acaggccgat ctgcgaggcc agctccatgt agagcaccgc cagcgagacc ggtatgccgc
     1861 ggcgcgtggc cagcacgcgg tgcagatagc tgttgtgcgg gtcgtagtag tcgttgacgt
     1921 tgcctgcgaa acccagctca tcgaagaaat agcggttcag caggcgcagc ttctgcagcg
     1981 ccccggcgtc gggggcgagg cgccgcctga gcttgtcggc cagcgcgtcg atctcggtca
     2041 gcacgccctg ggtgtcgagc ccggggaatt cgtcctgggc cagcgcaatg gccgcctccg
     2101 tgagcgccag gctcgcgtc
//
LOCUS       DASUCY010000232         2970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1081084, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000232 DASUCY010000000
VERSION     DASUCY010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081084"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..1505
                     /locus_tag="VFV25_04455"
     CDS             180..1505
                     /locus_tag="VFV25_04455"
                     /inference="COORDINATES: protein motif:HMM:NF024798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEY1392599.1"
                     /translation="MRSLMLTLGCVAVALTTALPAQAQGLVGEFTHCIGRLRSELKRH
                     PEIRAETFETYASSAQDLRPLIDKATSTQPEFTQSVWDYLARRVDAQRVVEGRDLLER
                     MAGALVVMQQRHAVDGATVVAVYGVETDYGRLRDKYPVVDATLGRACLNLASEERKSQ
                     LFAALWLLQQGYVKRDEFRGSWAGAFGLTQFMPATFVRYMDAADGSGAPDIMHSTLDA
                     LATTARYLRAQGWSDGLPWGIEVQVPRELALRANALEGDHACLVTAEQSGGSTGKCRH
                     IDEWITAGVKNAAGKALRTRDPAWPGLMGSTPAALLMPAGAAGPAWLVTSNYQAIWRY
                     NRADSYALAIGLLSDALRGGPGQRAGWPTDDPGLSRAQFRELQALLLRQGHCAMVVDG
                     AQGAITDAAIRQEEGRRGWPETGHPGMRLLAALRRDAQQAVARPCEKTQ"
     gene            complement(1533..1952)
                     /locus_tag="VFV25_04460"
     CDS             complement(1533..1952)
                     /locus_tag="VFV25_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3717 domain-containing protein"
                     /protein_id="HEY1392600.1"
                     /translation="MSGIHITDIEAAINWWRERSPSPDGIAACAEVRALAEVYALMVY
                     YHEPLCDEASLPAKAMQAWLAWYATTPDAPCIAICSTAQGDSVCKGCGRTEEEVQHWP
                     LMTPAEKRSTWRRIIMEDTAWRFNRYAERARQKTSDS"
     gene            complement(2026..>2970)
                     /locus_tag="VFV25_04465"
     CDS             complement(2026..>2970)
                     /locus_tag="VFV25_04465"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001093.1"
                     /note="catalyzes a two-step reaction, first charging a
                     tryptophan molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEY1392601.1"
                     /translation="AELFTLLAMGTPLGWLERVPTYKDQMDKLKDRDLATYGFLGYPL
                     LQAADILIYKAAYVPVGEDQASHVELTREVARRFNHLYGRATHFEERIAAALAKLGKD
                     DARYYEKQRKTFGETGAPEALAKGESIVNKAAAAIAGWSKDDSELLLGHLRGQGKIIL
                     PEPAALHTEVAKLPGLDGAKMSKSYGNTIAMREEPAEVERKIRRMPTDPARVHRSDPG
                     DPERCPVWQFHQVYSDAATKDWVVKGCKSAGIGCLECKQPVIDAIKKEQQPWRERAEA
                     YLSNPKQVQWIVEVGTERARTVAKQTMKEVRDAMGLSY"
BASE COUNT          445 a          996 c         1011 g          518 t
ORIGIN      
        1 tcgccacgcc gaagatgctc acgggagtcc actcctgtta tcgctcgaag aatagccaga
       61 gcagccgctg gggcctcgtg acttaggtaa tccatgacgg ggttatgtgt cgacaacggt
      121 gtccgctcta aactgcaccc ggtcccgttc tgcgcaagca aaagaggagc gttcatcgga
      181 tgcgaagctt gatgctcacg ctaggctgcg tggccgttgc cctgacgacg gcgctgccgg
      241 cgcaggccca ggggctggtg ggtgaattca cgcactgcat aggcaggctc aggagcgagc
      301 tcaagcggca tcccgagatc cgcgccgaga ccttcgagac ctatgcgagc agcgcccagg
      361 acctgcgtcc gctgatcgac aaggccacca gcacgcaacc cgagttcacg cagtcggtgt
      421 gggactatct ggcgcgccgc gtcgatgccc agcgcgtcgt cgaggggcgc gacttgctgg
      481 agcgcatggc tggcgccctg gtcgtgatgc agcagcgtca cgctgtggac ggcgcgaccg
      541 tggtggccgt gtacggcgtc gagaccgact acggacgcct gcgcgacaag tatccggtgg
      601 tcgatgcgac gctcggccgg gcctgcctca atctcgcgag cgaagagcgc aagagccagc
      661 tcttcgccgc gctgtggctg ctgcagcaag gctatgtgaa gcgggacgag ttccgcggct
      721 cctgggccgg cgccttcggc ctcacgcagt tcatgcccgc caccttcgtg cgctacatgg
      781 acgccgccga cggctcgggc gcacccgaca tcatgcacag tacgctcgat gcgctcgcca
      841 cgacggcccg ctatctgcgc gcccagggct ggagcgatgg cctgccctgg ggcatcgagg
      901 tgcaggtgcc gcgcgagctg gccctgcggg ccaatgccct ggaaggcgac catgcctgcc
      961 tggtcacagc ggagcagagc ggcggcagca ccggcaaatg ccgccacatc gacgaatgga
     1021 ttactgccgg cgtgaagaac gccgcaggca aggccctgag aacgcgtgat ccggcctggc
     1081 ccggcctcat gggctccacg cccgcagcct tgctgatgcc ggccggcgcc gccggcccgg
     1141 cctggctcgt gacgagcaac taccaggcga tctggcgcta caaccgggcc gacagctatg
     1201 ccctggcgat cggcctgctg tccgacgcgc tgcgcggcgg gcctgggcag cgcgccggtt
     1261 ggccgaccga cgacccgggg ctctctcgcg cgcagtttcg cgagctgcag gcgctgctgc
     1321 tgcggcaagg ccattgcgcg atggtggtcg acggggcgca gggggcgatc accgatgcgg
     1381 cgatccggca ggaggagggc cggcgcggct ggcccgagac cggccatccc ggcatgcggc
     1441 tgctcgctgc gctcaggcgc gacgcgcagc aggctgttgc tcgcccctgt gagaagaccc
     1501 agtgatcggt gggcaggtca cgataccgct gatcacgagt cgcttgtttt ttgccgcgca
     1561 cgctcggcat agcggttgaa gcgccacgcc gtgtcttcca tgatgatgcg gcgccaggtg
     1621 ctgcgcttct cggccggtgt catcagcggc cagtgctgca cctcttcctc ggtgcggccg
     1681 cagcccttgc agacgctgtc gccctgggcc gtcgagcaga tcgcgatgca gggcgcgtcc
     1741 ggcgtcgtcg cataccaggc gagccaggcc tgcatggcct ttgccggcag gctggcttcg
     1801 tcgcacagcg gttcgtggta gtacaccatc aaggcataga cctcggccag cgcacgcacc
     1861 tcggcgcagg ccgcgatgcc gtccggcgag ggcgagcgtt cgcgccacca gttgatggcg
     1921 gcctcgatgt cggtgatgtg gatgcctgac atcaaatagt gaccagtgac tggtgatccg
     1981 tgactggtga attcctccgg tcactagtca ccagtcacag gtcactcaat aagagagtcc
     2041 catcgcgtcg cgcacttcct tcatcgtctg cttggcgacg gtgcgcgccc gctcggtgcc
     2101 gacctcgacg atccattgca cctgcttggg gttgctcaga taggcctcgg cgcgctcgcg
     2161 ccagggctgc tgctccttct tgatcgcgtc gatcaccggc tgcttgcatt ccaggcagcc
     2221 gatgccggcg ctcttgcagc ctttaaccac ccagtccttg gtggcggcgt cggaatagac
     2281 ctggtggaac tgccacaccg ggcagcgctc gggatcgccg gggtcgctgc ggtgcacgcg
     2341 cgccgggtcg gtgggcatgc gcctgatctt gcgctcgacc tcggcgggct cctcgcgcat
     2401 cgcgatggtg ttgccatagc tcttgctcat cttggcgccg tccagccctg gcagcttggc
     2461 cacttcggtg tgcagcgcgg ccggctcggg caggatgatc ttgccctggc ccctcagatg
     2521 gccgagcagc agctcgctgt cgtccttgct ccagcccgcg atggcggccg cagccttgtt
     2581 gacgatggac tcgcccttgg ccagggcctc gggcgcgccg gtttcgccga aggtcttgcg
     2641 ctgcttctcg taatagcggg catcgtcctt gcccagcttg gccagcgcgg cggcaatcct
     2701 ctcctcgaaa tgggtcgcgc gaccgtacag gtgattgaag cggcgcgcca cctcgcgcgt
     2761 cagctcgaca tgcgaggcct ggtcctcgcc caccggcacg taggcggcct tgtagatcag
     2821 gatgtcggcc gcctgcagca gcggatagcc gaggaagcca taggtcgcga ggtcgcggtc
     2881 cttgagcttg tccatctggt ccttgtaggt cggcacgcgc tccagccagc ccagcggcgt
     2941 gcccatggcc agcagcgtga agagctcggc
//
LOCUS       DASUCY010000233         2968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1209836, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000233 DASUCY010000000
VERSION     DASUCY010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1209836"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VFV25_04470"
     CDS             <1..253
                     /locus_tag="VFV25_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEY1392602.1"
                     /translation="NEIQHWEFDEPRTEDADIRSASYATPSVYGFGHPLYYDNVIRTL
                     RGEAHAEVDGYEGLRSLEVIIAAYRSARDGVRVGLPLVF"
     gene            253..837
                     /locus_tag="VFV25_04475"
     CDS             253..837
                     /locus_tag="VFV25_04475"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEY1392603.1"
                     /translation="MSTAAWVHESAIVDAGAQLGEGTKVWHFSHVCAGAHIGAGCSLG
                     QNVFVGNDVRIGDNVRIQNNVSVYDAVALEDDVFCGPSVVFTNVYNPRAAVPRKAEYR
                     HTLVKQGATLGANCTIVCGATIGRHAFVGAGAVVQKDVPDFALVVGVPAHRIGWMSRH
                     GERLDLPSTGHGEALCPATGERYRLSGETLSLVE"
     gene            852..1982
                     /locus_tag="VFV25_04480"
     CDS             852..1982
                     /locus_tag="VFV25_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEY1392604.1"
                     /translation="MQFIDLKSQYAALKTSIDTRIQRVLDHGQYIMGPEVRELEEALV
                     AYTGAKHCITVASGTEALLISLMALDLKPGDEVITTPFTFAATAEMIVLLGGKPVFVD
                     IEPDTCNIDAALIEAQITGRTKAIMPVGLYGQVADMDAINAIAQRHGLAVIEDAAQSF
                     GAIYQGRKSCNLSTFGCTSFFPSKPLGCYGDGGAIFTSDDALAQACRELRVHGQSGRY
                     HHTRVGVGGRMDTLQCAVVLAKLERFEWELQQRRVIGERYNRLLADLPGVQRLAVRPD
                     RDCVWAQYTVFVPERAALQAALQAAGIPTAVHYPKPLHRQPAYAVHGAGVHCPHSEVA
                     AEHVLSLPMSADLSEADQDRIVAALRAALASAPAPAPTRRAA"
     gene            1989..>2968
                     /locus_tag="VFV25_04485"
     CDS             1989..>2968
                     /locus_tag="VFV25_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEY1392605.1"
                     /translation="MALAASMHDQAPSPGLLRAVLTLLAGGVMAQALPLLLGPLLTRL
                     YTPQEFGIYHLFAAVAANLAVVACARYEFALPMAREDSEAQALRALCLWILAGVSFAS
                     LLGGVAWAVAAGAAWPLYLPLAVAMLGALSLATLSATRAQRYRSLAAARALQYGGGAL
                     AQAGAGWAQAGVAGLIVGPIAAAGLALLALRLRIAAWWQTPRAGLREAARRYREFPLL
                     NTPHAFAGALQDTLSVALIAAALGPAAAGFWGLALRYLKAPATLVGGAVSQALYPRLA
                     HDGRGATPEARRAVARVMVVLALIATPLVLLLWLLGPWLFEIAFGAQWRAA"
BASE COUNT          421 a         1022 c         1037 g          488 t
ORIGIN      
        1 caacgagatc cagcactggg aattcgacga gccgcgaacc gaggatgccg acatccgatc
       61 cgccagctac gcaacgcctt cggtctacgg cttcggccat ccgctgtact acgacaacgt
      121 gatccgcacg ctgcgcggcg aggcgcatgc cgaggtcgac ggctatgaag gcctgcgctc
      181 gctcgaagtc atcatcgccg cctaccgctc ggcgcgcgac ggtgtgcgcg tcggtctgcc
      241 cctggtgttt tgatgagcac tgccgcctgg gtgcacgaaa gcgccatcgt cgatgccggc
      301 gcccagctgg gcgagggcac caaggtctgg catttcagcc atgtctgtgc cggtgcgcac
      361 atcggcgcgg gctgctcgct gggccagaac gtcttcgtcg gtaatgacgt gcgcatcggc
      421 gacaacgtgc gcatccagaa caacgtgtcg gtgtacgacg cggtggcgct ggaggacgac
      481 gtgttctgcg gccccagcgt ggtgttcacc aacgtctaca acccgcgcgc agccgtgccg
      541 cgcaaggccg agtaccgcca cacactggtg aagcaggggg ccacgctggg cgccaactgc
      601 accatcgtct gcggcgcgac cataggccgc catgccttcg tcggcgccgg cgcggtggtg
      661 cagaaggacg tgcccgactt cgcgctggta gtcggcgtgc cggcgcaccg catcggttgg
      721 atgagccgcc acggcgagcg gctggacctg ccttcgacgg gacacggcga ggcgctgtgc
      781 cctgccaccg gcgagcgcta tcggctcagc ggcgaaacgc tgagcctcgt ggaatgagcc
      841 aggcaacgac gatgcaattc atcgacctca agtcgcaata cgcggccctc aagacttcca
      901 tcgatacgcg catccagcgc gtgctcgacc atggccagta catcatgggc ccggaggtcc
      961 gcgaactgga agaggccttg gtcgcctaca ccggcgccaa gcactgcatc accgtggcca
     1021 gcggcaccga ggcgctgctg atctcgctga tggcgctcga cctgaagccc ggcgacgagg
     1081 tcatcaccac gcccttcacc ttcgccgcca ccgccgagat gatcgtgctg ctgggcggca
     1141 agccggtgtt cgtcgacatc gagcccgata cctgcaacat cgatgctgcg ctcatcgagg
     1201 cgcagatcac gggccgcacc aaggccatca tgccggtggg cctgtacggc caggtggccg
     1261 acatggacgc gatcaacgcc attgcacagc gccatggtct cgcggtgatc gaggatgcgg
     1321 cgcagagctt cggcgccatc tatcagggcc gcaagagctg caacctctcc accttcggtt
     1381 gcaccagctt cttccccagc aagccgctgg gctgctatgg cgacggcggc gcgatcttta
     1441 ccagtgacga tgcgctggcc caggcctgcc gcgagctgcg cgtgcatggc cagagcggcc
     1501 gctatcacca tacccgcgtc ggtgtcggtg gccgcatgga cacgctgcaa tgcgcggtgg
     1561 tgctggccaa gctggagcgc ttcgaatggg agctgcagca gcggcgcgtc atcggcgagc
     1621 gctacaaccg gctgctggcc gacctgccgg gcgtgcagcg cctcgcagtg cggcccgacc
     1681 gcgactgcgt gtgggcgcag tacacggtct tcgtgcctga gcgcgccgcg ctgcaggccg
     1741 cgttgcaggc cgccggcatc ccgacagcgg tgcattaccc caagccgctg catcgccagc
     1801 ctgcctatgc agtccacggc gcaggcgttc actgtccgca tagcgaggtg gcagccgagc
     1861 acgtgctcag cctgccgatg agtgccgacc tgagcgaggc cgaccaggat cgcatcgtcg
     1921 cggcgctgcg tgcggccctg gcctcggcac cggcgccggc acctacacgt cgggccgcct
     1981 gaggccgcat ggcgctcgcc gcctcgatgc acgatcaagc cccgtcgccc ggcctgttgc
     2041 gcgcagtgct gacgctgctg gccggaggcg tgatggctca ggcgctgccg ctgttgctcg
     2101 ggccgctgct cacgcggctg tacacgccac aggagttcgg catctatcac ctcttcgccg
     2161 cggtggcggc caatctcgcg gtggtggcct gcgcgcgcta cgagttcgca ctgccgatgg
     2221 cgcgcgagga cagcgaagcc caggccttgc gcgcgctgtg cctgtggatc ctcgcgggcg
     2281 ttagcttcgc ctcgctgctg ggcggcgtgg cgtgggccgt ggccgcggga gcggcgtggc
     2341 cgctgtatct gccgctggcg gtcgccatgc tgggcgctct gtcgctggca acgctgtcgg
     2401 cgacgcgggc ccagcgctac aggtcgctgg ccgcggcgcg cgcgctgcaa tacggcggcg
     2461 gcgcgctggc ccaggccggt gccggctggg cacaggcagg tgtcgcgggc ctgatcgtgg
     2521 gcccaattgc ggcggcaggc ctcgccttgc tggcgttgcg tttgcgtatc gccgcctggt
     2581 ggcaaacgcc acgcgccgga ctgcgcgaag ccgcacggcg ctatcgcgag ttcccgctac
     2641 tgaacacgcc gcatgctttc gccggcgcgt tgcaggacac gctgtcggtg gcgctgatcg
     2701 ccgctgcgct gggcccggcg gcagccggct tctggggctt ggcgctgcgt tatctcaagg
     2761 cgccggccac gctggtcggc ggcgccgtct ctcaggcgct gtatccgcgg ttggcccatg
     2821 acggccgcgg tgcgacgccc gaggcgcgcc gtgctgtcgc gcgcgtgatg gtggtgctgg
     2881 ccctgatcgc gacgccgctg gtgctgctgc tgtggttgct gggcccgtgg ctcttcgaga
     2941 ttgctttcgg cgcgcaatgg cgtgcggc
//
LOCUS       DASUCY010000234         3684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_502833, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000234 DASUCY010000000
VERSION     DASUCY010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502833"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..511
                     /gene="ndk"
                     /locus_tag="VFV25_04490"
     CDS             86..511
                     /gene="ndk"
                     /locus_tag="VFV25_04490"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEY1392606.1"
                     /translation="MGVERTLSIIKPDAVAKNVIGQIYSRFEAAGLKIIAVKMAHLSR
                     GEAEAFYAVHKARPFFNDLVNFMISGPVVIQVLEGDNAIIKNRELMGATDPKKAEKGT
                     IRADFADSIDANAVHGSDAPETAAVEVAFFFPGMNVYSR"
     gene            565..1716
                     /gene="rlmN"
                     /locus_tag="VFV25_04495"
     CDS             565..1716
                     /gene="rlmN"
                     /locus_tag="VFV25_04495"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEY1392607.1"
                     /translation="MTAANLLDFDLDGLAAFCEGLGEKRFRATQLFRWIHQKGASDFE
                     QMSDLAKSLRGKLAGAAEVRPLTVISEHASADGTVKWLFDVGGGNAVETVFIPEDDRG
                     TLCVSSQAGCAVGCRFCSTGHQGFSRNLTTGEILAQLWHAEHALRARLKPKAGERGEG
                     DDKRVVTNVVMMGMGEPLQNYGALVPALRVMLDDHGYGLSRRRVTVSTSGVVPMIDRL
                     REDCPVALAVSLHAPNDALRDELVPLNRKYPIDELLDACLRYLDKAPRDFITFEYCML
                     DGVNDTPAHARELVELVRGRVSCKLNLIPFNPFPQSGLRRSSNARVQAFAQVLIEAGI
                     VTTVRKTRGDDIDAACGQLAGEVQDRTHAKTRMTRAPITIVAKNTENRT"
     gene            1713..2561
                     /gene="pilW"
                     /locus_tag="VFV25_04500"
     CDS             1713..2561
                     /gene="pilW"
                     /locus_tag="VFV25_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HEY1392608.1"
                     /translation="MKLGYLQQRAWMAWAALICSALLAACAAPGTGSTSNRSSASSDA
                     GNGRDLMTESDETDTHKRARIRLELATAYFSQGQSTTALDEVKQALAVDPSLAGAYNL
                     RGLIYASLNDDGLAEESFRRALQLSPDDGSAMHNYAWFMCQRRRFDEADAMFGRAAAL
                     PQYRSLGMTLMAQGVCQARAGKSADAEKTLLHAFEVDPSSPATAMNLADLLYRKGDYE
                     RARFYVRRVNNVPDLSNAETLWLAARIEQRMGNRQGANDLGAQLRARYPNSREAAAFD
                     QGRFNE"
     gene            2554..3570
                     /locus_tag="VFV25_04505"
     CDS             2554..3570
                     /locus_tag="VFV25_04505"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY1392609.1"
                     /translation="MSESEVVADPASPTETGDAELSAGALLRKARQAQGMHIAALAAA
                     IKVTPQKLEALEADRHEELPDATFVRALAQTVCRTLKIDPEPVLAKLPRPAQSLRLEE
                     VTQGLKMPFKDRNSRGEGFDAGMLLQARFWAPLLLLVAAALVWLLPPEVKHLMKGRLA
                     APQTHEAAQAASAASAAVAASMPEAASGLAPLTQEAAPVPGSQSLPPAVTPASTPAPA
                     MPAAAREALVPQPAAVAPEAASPSASEAASQAKPLAGSAQPGGALQLRTTEESWVEIR
                     DGNGKLLVSRQLAAGESVALSGATPLRLRIGNPSAAHLSFRGKPVDLAPYTTRDNLAK
                     LELK"
BASE COUNT          644 a         1278 c         1221 g          541 t
ORIGIN      
        1 cgctgagctg cggtttcaag agaagaaacc gagccagacg ctacaatcaa aagctttgct
       61 aaatccttga ttccggagaa aaaacatggg tgtggaacgc actttgtcga tcatcaagcc
      121 tgatgccgtg gccaagaacg tgatcggcca gatctacagc cgcttcgaag ccgcgggtct
      181 gaagatcatc gccgtcaaga tggcgcattt gtcgcgcggc gaggccgagg ctttctacgc
      241 cgtgcacaag gcgcgcccct tcttcaacga cctggtcaac ttcatgatct cgggcccggt
      301 cgtgatccag gtgctcgaag gcgacaacgc catcatcaag aaccgcgagc tgatgggtgc
      361 caccgacccg aagaaggccg agaagggcac cattcgcgcc gatttcgccg acagcatcga
      421 cgccaatgcg gtgcatggct ccgacgctcc cgaaaccgca gccgtggaag tggccttctt
      481 cttccccggc atgaacgtct acagccgctg aacctcagcc cgggccagcc ctccaagcga
      541 ggactgcccg atgtgacgaa gactatgacc gcggccaacc tgctcgattt cgatctcgac
      601 ggcttggccg cgttctgcga aggtctgggc gagaagcgct tccgcgccac ccagctcttc
      661 cgctggatcc accagaaggg cgcgtccgac ttcgagcaga tgtcggacct cgccaagtcg
      721 ctgcgcggca agctcgccgg cgccgccgaa gtgcggccgc tgaccgtgat cagcgaacac
      781 gcctccgccg acggcaccgt gaaatggctg ttcgacgtgg gcggcggcaa cgcggtggag
      841 acggtcttca ttcccgagga cgaccgcggc acgctgtgcg tctcctcgca ggcgggctgt
      901 gcggtgggtt gccgcttctg ctccaccggc caccagggct tcagccgcaa cctcaccacc
      961 ggcgagatcc tggcccagct ctggcatgcc gagcacgcgc tgcgtgcgcg cctgaagccc
     1021 aaggccggcg agcgcgggga aggggacgac aagcgtgtcg tgaccaacgt cgtgatgatg
     1081 ggcatgggcg agccgctgca gaactacggc gccctcgtgc cggcgctgcg cgtgatgctc
     1141 gacgaccacg gctacggcct gtcgcgccgc cgcgtgacgg tctcgacctc gggcgtggtg
     1201 ccgatgatcg atcgcctgcg cgaggactgc ccggtcgcgc tggccgtctc gctgcacgca
     1261 cccaacgatg cgctgcgcga cgagctggtg ccgctgaacc gcaaataccc gatcgacgag
     1321 ctgctcgatg cctgcctgcg ctacctcgac aaggcgccgc gcgacttcat caccttcgag
     1381 tactgcatgc tcgacggcgt gaacgacacc ccggcccatg cgcgcgaact ggtcgagctg
     1441 gtgcgcggcc gcgtgtcctg caagctcaac ctgatcccct tcaatccctt cccgcaatcg
     1501 ggactgcggc gttcgtccaa cgcccgtgtg caggctttcg cccaggtgct gatcgaagca
     1561 ggtatcgtca cgaccgtgcg caagacacgc ggcgacgaca tcgatgcggc ctgcggccag
     1621 ctggccggcg aagtgcagga ccgcacccat gcgaagaccc gcatgacacg cgcgccgatc
     1681 accatcgtgg cgaagaacac ggagaacaga acatgaagtt gggatatctg cagcagcggg
     1741 cctggatggc atgggccgcg ctgatttgtt cggccctgct ggccgcctgc gcggcacccg
     1801 gcacgggcag cacgtcgaac cgatcgtcgg catcctccga tgccggcaac gggcgcgacc
     1861 tcatgacgga atcggacgag accgataccc acaagcgcgc gcgcatccgc ctcgaactgg
     1921 cgaccgccta tttcagccaa ggccagtcga ccacggccct ggacgaagtg aagcaggctc
     1981 tggcggtgga tcccagcctg gccggggcct acaacctgcg tggcctgatc tatgcgagcc
     2041 tcaacgacga cggcctcgcc gaagagagct tcaggcgggc gctgcagttg agccccgacg
     2101 acggcagcgc tatgcacaac tacgcctggt tcatgtgcca gcggcgccgt ttcgacgagg
     2161 cggacgcgat gttcgggcgg gcggccgcgc tgccgcaata ccgcagcctc ggcatgacgc
     2221 tgatggccca gggcgtatgc caggcgcgcg ccggcaagtc ggccgacgcc gagaagacgc
     2281 tgctgcatgc cttcgaggtc gacccgagca gcccggccac ggcgatgaat ctcgccgatc
     2341 tgctctatcg caagggcgac tacgagcggg cgcgcttcta cgtgcgccgc gtcaacaatg
     2401 tgcccgacct gtccaatgcc gagacgctgt ggctggcggc gcgcatcgaa cagcgcatgg
     2461 gcaaccgcca gggcgccaac gacctgggcg cgcagctgcg cgcccgctac ccgaactcgc
     2521 gcgaggccgc ggccttcgac caggggcggt tcaatgagtg agagcgaggt ggtggcagat
     2581 cccgcgtccc cgaccgaaac aggcgatgcc gagctgagcg caggcgctct gctgcgcaag
     2641 gccaggcagg cccagggcat gcacatcgcg gccctggccg cggccatcaa ggtgacgccg
     2701 cagaagctgg aggccctgga ggccgaccgg cacgaggaat tgccggatgc cacttttgtc
     2761 cgcgccctgg cgcagacggt gtgccggacg ctgaagatcg accccgagcc ggtgctggcc
     2821 aagctgccgc gccctgctca gtctctgcgc ctggaagagg tcacccaagg cctcaaaatg
     2881 cctttcaagg accgtaatag ccgcggcgaa ggcttcgatg ccggcatgct gctgcaggcc
     2941 cgcttctggg cgcccctgtt gctgctcgtg gcggcagcgc tggtctggct gctgcccccc
     3001 gaggtgaagc atctgatgaa aggcagactg gcagcgccgc agactcacga ggcggctcag
     3061 gcggcttcgg ccgcgagtgc ggcagtggca gcgtcgatgc ccgaggccgc gagcggcctc
     3121 gccccgctga cgcaggaagc agcgccggtg cccggctcgc agagcctgcc gccggccgtc
     3181 acgcccgcct cgacgccggc gcctgcgatg cccgcggctg cacgcgaggc gctggtgccg
     3241 cagccggccg ccgtcgcgcc ggaagccgcc agtccatcgg cttccgaggc ggcctcgcag
     3301 gccaagccgc tggctggtag cgcacagccc gggggcgcgc tgcagctgcg caccaccgaa
     3361 gagtcctggg tcgagatccg cgacggcaac ggcaagctgc tggtctcgcg ccagctggcg
     3421 gccggcgagt cggtagccct gagcggcgca acgccgctgc ggctgcgcat cggcaatccg
     3481 agtgccgcgc atctgagttt tcgcggcaag ccggtcgacc tggcgccgta tacgacgcga
     3541 gacaacctcg ccaagctgga acttaagtaa gtgaagcccg cagacaaccg gcccgctgtc
     3601 gccgaagcct tcgacactgc catcgccgcc gccgctccgg ccgcgcgacg ctcgcgccag
     3661 gcgcgcgtcg cctggggctc gcat
//
LOCUS       DASUCY010000235         2124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1273980, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000235 DASUCY010000000
VERSION     DASUCY010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1273980"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VFV25_04510"
     CDS             <1..401
                     /locus_tag="VFV25_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEY1392610.1"
                     /translation="NWLKRVSAEIDSGCIYISGAVEFDDRPGPVRDALVAMVRTWQQA
                     LERAIHAAVTEGHLRPETEAQQLLFELHGLVLALHHDARFLQTPGAVERARVAFERLV
                     DHHATPAGRRTAGAAAPRKAGPTRHRKPEA"
     gene            449..>2124
                     /locus_tag="VFV25_04515"
     CDS             449..>2124
                     /locus_tag="VFV25_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY1392611.1"
                     /translation="MPHYAPPLRDMQFVLHELLDVGTELKRLPAHADVDAETINAVIE
                     EAGKFAAEVVAPLNPIGDLEGCKLDKATHAVTTAKGFKEAYAQYVAGGWPALSSDPAY
                     GGQGLPHVVSQCLFEMLNSANQAWTMYPGLSHGAYEALHAHGTDEQKKLYLPKLTSGE
                     WTGTMCLTEPHCGTDLGLLRTRAEPQPDGSYKLTGNKIFISAGEHNMTSNIVHLVLAR
                     LPDAPQGSKGISLFAVPKFNVNPDGSLGSRNGIYCGGLEHKMGIHGNATAQMVLEGAV
                     GTMVGAPNKGLAAMFVMMNAARLGVGNQSLGLTEVAYQNALAYAKDRIQMRSLSGVKA
                     KDMPADPIIVHPDVRKMLLTAKAYAEGGRALAIYCTLLLDKEQHHPDEKVRKDSAEVV
                     ALLTPIVKAFLTDNGHIATNACMQVFGGHGFIKEWGMEQFVRDARINMIYEGTNGIQA
                     LDLLGRKILGNNGATLKKFGKLVAALILEEGVNEKMAEFINPLAYLADQMTKFTTELG
                     FKGFQNPDEVGAAAVDYLRVAGHLVFGYFWARMAQVALREIAAGNTDKFYV"
BASE COUNT          394 a          742 c          673 g          315 t
ORIGIN      
        1 agaattggct gaagcgcgtc tcggcggaga tcgattcggg ctgcatctat atcagcggcg
       61 cggtcgagtt cgacgaccgg ccggggcccg tgcgcgatgc gctggtcgcc atggtgcgca
      121 cctggcagca ggcgctcgag cgcgcgatcc atgcggccgt caccgaaggc cacctccgcc
      181 ccgagaccga ggcccagcag ctcctgttcg aattgcacgg cctcgtcctc gccctgcacc
      241 acgacgcgcg cttcctgcag acgcccggcg ccgtcgagcg cgcacgcgtc gccttcgagc
      301 gcctcgtcga ccaccacgcc acgcctgccg gccggcgcac cgcgggtgcc gcggcgccgc
      361 gcaaggccgg ccccacccgc catcgcaagc ccgaggcctg agcctcgttc cgggaagccc
      421 tcgcctcgac ccgccaagga gcctcgtcat gccccactac gccccgccgt tgcgcgacat
      481 gcagttcgtc ctccacgagc tgctcgacgt cggcaccgag ctgaagcgcc tgcccgcgca
      541 tgccgacgtc gacgccgaga cgatcaacgc cgtcatcgag gaggcgggga agttcgcggc
      601 ggaggtggtg gcgccgctga accccatcgg cgacctcgag ggctgcaagc tcgacaaggc
      661 gacgcatgcc gtcaccacgg ccaagggctt caaggaggcc tacgcgcagt acgtcgccgg
      721 cggctggccg gcgctgagct ccgatcccgc ctacggcggc caggggctgc cgcacgtcgt
      781 gagccagtgc ctcttcgaga tgctcaactc ggccaaccag gcctggacca tgtacccggg
      841 cctgtcgcac ggcgcctacg aggccctgca tgcccacggg accgacgagc agaaaaaact
      901 ctacctgccc aagctgacca gcggcgaatg gaccggcacc atgtgcctga ccgagccgca
      961 ctgcggcacc gacctgggcc tgctgcgcac ccgggccgaa ccccagcctg atggcagcta
     1021 caagctcacc ggcaacaaga ttttcatcag cgccggcgaa cacaacatga ccagcaacat
     1081 cgtgcacctg gtgctggcgc gcctgcccga cgcgccccag ggcagcaaag gcatcagcct
     1141 gttcgccgtg cccaaattca acgtcaaccc cgacggctcg ctgggatcgc gcaacggcat
     1201 ttactgcggc ggcctggagc acaagatggg catccatggc aacgccaccg cgcagatggt
     1261 gctcgagggc gccgtgggca ccatggtggg tgcgcccaac aagggcctgg ccgcgatgtt
     1321 cgtgatgatg aacgcggcgc gcctgggcgt cggcaatcag tcgctgggcc tgaccgaagt
     1381 ggcctaccag aacgcgctgg cctatgccaa ggaccgcatc cagatgcgtt cgctgtcggg
     1441 cgtgaaggcc aaggacatgc cggccgaccc tatcatcgtg caccccgacg tgcgcaagat
     1501 gctgctgacc gccaaggcct acgccgaagg tggccgcgcg ctggccattt actgcacgct
     1561 gctgttggac aaagagcagc accaccccga cgagaaagtg cgcaaggatt cggccgaggt
     1621 cgtcgcgctg ctcacgccca tcgtgaaggc tttcctgacc gacaacggcc atatcgcgac
     1681 caatgcctgc atgcaggtct ttggcggcca cggcttcatc aaggaatggg gcatggagca
     1741 gtttgtgcgc gatgcgcgca tcaacatgat ctatgaaggc acgaacggca tccaggcgct
     1801 ggatctgctg ggtcgcaaga tcctgggcaa caacggcgcc accctgaaga aattcggcaa
     1861 gctggtcgcc gcgctgattc tagaggaagg cgtcaacgaa aagatggccg agttcatcaa
     1921 tcccttggcc tacctggccg accagatgac caagttcacc accgaactgg gcttcaaggg
     1981 tttccagaat cctgatgaag tgggcgccgc tgcggtggac tacctgcggg tggccggcca
     2041 cctggtgttc ggctacttct gggcccgcat ggcgcaagtc gcgctgcgcg agattgccgc
     2101 cggcaacacc gacaagttct atgt
//
LOCUS       DASUCY010000236         3204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_342237, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000236 DASUCY010000000
VERSION     DASUCY010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342237"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1194)
                     /locus_tag="VFV25_04520"
     CDS             complement(<1..1194)
                     /locus_tag="VFV25_04520"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HEY1392612.1"
                     /translation="MRPAHLLGAAALALSACAAATTVEPVDARLTITQEAAGPRIEPE
                     VYGQFAEHLGTGIYGGLWVGPESKIPNTRGWRNDVVDALRKLQVPVVRWPGGCFADDY
                     DWHDGIGNPAKRPARLNKIWGNVIEPNRVGTHEYMDLVEQLGAQAYIAGNMGSMPPRA
                     MAQWLEYMTSDSKSSLAEERRRNGRDKPWKVKYFGIGNESWGCGGSMRPEYQADLHNR
                     YETFLHAPVVRVASGDGQGNDHLIEVLMERSGPHLDALTLHYYTMPGDWNQHGSATGF
                     DAPAWAKTLKSAIGIEPRIEAVSRLMDKHDPAKRVALYVDEWGTWYDADPGTRPGFLQ
                     QQNTLRDALVAALSFNSFHRHTERVKMANIAQMVNVLQALVLTDGARMVLTPTYHAFD
                     LYKPFR"
     gene            complement(1202..2698)
                     /locus_tag="VFV25_04525"
     CDS             complement(1202..2698)
                     /locus_tag="VFV25_04525"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEY1392613.1"
                     /translation="MGIPFDRIVVVGGGTAGWMTAAAIATALRGRCTVEVVESEEIGI
                     VGVGEATFPSIRAYNKIVGIDEAEFLRATNGTYKLGIEFRDWRVQGESYFHTFGDFGE
                     LIGPHALWGQHRRAADPALGSLGEQCLPTVMAMQGRFVVPDEQAGTPYKYAYHFDATL
                     YAAFLRDLALSRGARRTEGRIVDVARRPDGGVHSLKLADGREITGDLFIDCSGFGSLL
                     LGRTLGEPFIDFSRWLPVDGAWACPCEREGSELTPYTRSTALEGGWAWRIPLQSRTGH
                     GHVFSSRYIDVDRAREQLLQQLDGKPLAEPRLLRFTTGHRERFWVHNVVALGLSSGFL
                     EPLESTSIFLIQSGLGRLMTLLSAGQPIIGDTVKDFNARALREFRRIRDFILLHYCLT
                     ARSDSALWRDMATMELPETLAFKIEAWRRFGVLHIQQDEGFDETSWLAIHAGMGHWPE
                     RADPVLEEFPRDEALQALLRRKQSIAAAVARLPTHDAYLNHILHSRRN"
     gene            complement(2732..>3204)
                     /locus_tag="VFV25_04530"
     CDS             complement(2732..>3204)
                     /locus_tag="VFV25_04530"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEY1392614.1"
                     /translation="SPLAPDQPVIAYPPINRTALASDPINVTTNRPTSTSGAEAGHTA
                     PMANDGQEQTWWRAAAKDGPAFWAVHLENVYALHWLSLVMPDDRGPDFVVEISKDAAN
                     WRQIAEAKGGRKTYEFALFEQAVTASFLRIRFPSVTPDHPAALSEVRVMAHPAN"
BASE COUNT          454 a         1060 c         1152 g          538 t
ORIGIN      
        1 gcggaacggc ttgtataggt cgaacgcgtg gtaggtcggc gtcagcacca ttcgcgctcc
       61 gtcggtcagc accagcgcct gcagcacgtt gaccatctgc gcgatgttgg ccatcttcac
      121 gcgctcggtg tggcggtgaa agctattgaa gctcagcgcc gccaccagcg catcgcgcag
      181 cgtgttctgc tgctgcagga agccggggcg ggtgcctgga tccgcgtcgt accaggtgcc
      241 ccattcgtcg acgtacagcg cgacgcgctt ggccggatcg tgcttgtcca tcaggcgact
      301 taccgcctcg atgcgtggct cgatgccgat ggcgctcttc agcgtcttcg cccaggccgg
      361 cgcatcgaag ccggtggcgc tgccgtgctg gttccagtcg ccgggcatcg tgtagtagtg
      421 cagcgtgagc gcgtccaggt gcgggccgga gcgctccatc agcacctcga tcaggtggtc
      481 gttgccttgg ccgtcgccgg aggccacgcg cacgaccggg gcgtgcagga aggtttcgta
      541 ccggttgtgc aggtcggcct ggtattcggg ccgcatgctg ccgccgcagc cccagctctc
      601 gttgccgata ccgaagtact tcactttcca cggcttgtcg cggccgttgc ggcggcgctc
      661 ctcggccagg ctcgacttgc tgtccgaggt catgtactcg agccactggg ccatcgcgcg
      721 cggcggcatc gagcccatgt tgcccgcgat gtaggcctgg gcgccgagct gctcgacgag
      781 gtccatgtac tcatgcgtgc cgacgcggtt gggctcgatc acgttgcccc agatcttgtt
      841 cagccgcgcc gggcgcttgg ccgggttgcc gatgccgtcg tgccagtcgt agtcgtcggc
      901 gaagcagccg ccgggccagc gcacgacagg tacctgcagc ttgcgcaggg catccaccac
      961 gtcgttgcgc cagcctcgcg tgttggggat cttcgattcc gggccaaccc agaggccgcc
     1021 gtagatgcca gtgcccaggt gctcggcgaa ttgcccgtag acctcgggct cgatgcgcgg
     1081 gccggccgct tcctgggtga tcgtgaggcg cgcgtcgacg ggttcgacgg tggtcgccgc
     1141 ggcgcaggca gacagcgcca gggccgcagc gcccagcagg tgtgcgggtc tcatcgggag
     1201 ctcagttcct tcgcgaatgc aggatgtggt tcaggtaggc gtcgtgcgtc ggcagccgcg
     1261 cgaccgccgc ggcgatcgat tgcttgcggc gcagcagggc ctgcaaagcc tcgtcgcgcg
     1321 ggaactcctc caacaccggg tcggcgcgct ccggccaatg gcccatgccg gcgtggatgg
     1381 ccagccagct cgtctcgtcg aagccttcgt cctgctggat gtgcagcacg ccgaagcggc
     1441 gccaggcctc gatcttgaag gccagggtct cgggcagctc catagtggcc atgtcgcgcc
     1501 acagcgcgct gtcgctgcgc gcggtcaggc agtagtgcag gaggatgaag tcgcggatgc
     1561 gcctgaactc gcgcaacgcg cgtgcgttga agtccttcac cgtgtcgccg atgatcggct
     1621 gcccggcgct gagcagcgtc atcaggcgtc cgaggccgct ctggatgagg aagatgctgg
     1681 tcgattccag cggctccagg aagcccgacg acaagccgag ggcgaccacg ttgtgcaccc
     1741 agaagcgctc gcgatggccg gtggtgaagc gcagcagccg cggctcggcc agcggcttgc
     1801 cgtccagttg ctgcagcagt tgctcgcgcg cgcggtcgac gtcgatgtag cggctgctga
     1861 agacatggcc gtgcccggtg cggctctgca gcggaatgcg ccaggcccag ccgccttcga
     1921 gcgcggtgga gcgggtgtag ggcgtgagct cgctgccctc gcgctcgcaa gggcaggccc
     1981 aggcgccgtc caccggcagc cagcggctga aatcgatgaa gggctcgccc agcgtgcggc
     2041 ccagcagcag cgagccgaag cccgagcagt cgataaacag gtcgccggtg atctcgcggc
     2101 cgtcggccag cttcaggctg tgcacgccgc cgtcggggcg acgcgccacg tcgacgatgc
     2161 gcccctcggt gcggcgggcg ccgcggctga gcgccaggtc gcgcaggaag gcggcataga
     2221 gggtcgcgtc gaagtggtag gcgtacttgt agggcgtgcc ggcctgctcg tccggcacca
     2281 cgaagcggcc ctgcatggcc atcaccgtag gcaggcattg ctcgccgagg ctgcccagcg
     2341 ccggatcggc cgctcgccgg tgctgacccc acagcgcatg cgggccgatg agctccccga
     2401 agtcgccgaa agtgtggaag tagctctcgc cctgcacgcg ccagtcgcgg aactcgatgc
     2461 cgagcttgta ggtgccgttc gtcgcgcgca ggaattcggc ctcgtcgatg ccgacgatct
     2521 tgttgtaggc gcgaatcgac gggaaggtgg cctcgccgac gccgacgatg ccgatctcct
     2581 ccgactcgac aacctcgacc gtgcagcggc cgcgcagagc ggtcgcgatg gcggctgcgg
     2641 tcatccagcc ggcggtgccg ccgccgacga cgacgatgcg atcgaacggg atgcccatgc
     2701 gctgctcctc gtggtcagcc agttgccgtc ttcagttcgc gggatgcgcc atcacccgca
     2761 cctcgctcag cgcggccggg tggtcgggcg tgaccgacgg gaagcggatg cgcaggaaac
     2821 tcgcggtgac cgcctgctcg aagagtgcga actcgtaggt cttgcgtccg cccttggcct
     2881 cggcgatctg gcgccagttg gcggcgtcct tgctgatctc gacgacgaag tcgggccccc
     2941 ggtcgtccgg catgaccagc gacagccaat ggagggcgta cacgttctcc aggtggacgg
     3001 cccagaacgc ggggccgtcc ttcgcagccg cgcgccacca ggtctgctcc tggccgtcgt
     3061 tggccatcgg cgccgtgtgg ccggcctcgg cgccgctggt cgacgtcggc cggttggtgg
     3121 tgacgttgat cggatcgctg gcgagagcgg tacggttgat cggcgggtag gcgatgacgg
     3181 gctggtcggg cgccagcggc gacc
//
LOCUS       DASUCY010000237         4387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_374425, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000237 DASUCY010000000
VERSION     DASUCY010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374425"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1171
                     /locus_tag="VFV25_04535"
     CDS             <1..1171
                     /locus_tag="VFV25_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEY1392615.1"
                     /translation="AYLRHVDPTGELSSEALKAAVHLRPTTDAAALKDADFIVVAVPT
                     PVDDAHNPDFSPLVGASIAVGKNLKRGATVVFESTVYPGATEDECIPLIERHSGLKWK
                     QDFFVGYSPERINPGDKERTLTRITKVVSGDTPQTLELVAEIYGSIITAGVHKASSIK
                     VAEAAKVIENTQRDLNIALVNELSLIFHRIGIDTLEVLQAAGTKWNFLPFRPGLVGGH
                     CIGVDPYYLTHKAERLGYHPQVILAGRRINDGMGKYVAEQTVKQMIQAGVSVKGSHVI
                     VLGLTFKENCPDLRNSKVIDVVRELESYGVTVHVHDPVAAPAEARHEYGVELASWEHL
                     PRANAIVAAVSHKELVARPLDDYVAKLAPGGLFVDVKCQADAQALRARGINVWRL"
     gene            1171..2199
                     /locus_tag="VFV25_04540"
     CDS             1171..2199
                     /locus_tag="VFV25_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY1392616.1"
                     /translation="MQLSPELSAALAASPRRWLVTGAAGFIGSHLLEVLLGAGQQVTT
                     LDNFATGRQANLDEVKRKVGEAAWARHRFIEGDIADPAICRSACEGVELVLHQAALGS
                     VPRSIKDPLATHRANATGFLNMLVAAQGEGVARFVYAASSSTYGDSPTLPKVEDRIGR
                     PLSPYAVTKYLNELYADVFARCYGVSAIGLRYFNVFGARQDPNGAYAAVIPRWAAQIL
                     HGEACVINGDGETSRDFCYIANAVQANLLAALTDKPEAQNQVYNVAVGQRTTLKQLHA
                     ALAAELQSLRPGLAIAPPVHAEFRSGDVRHSLADVSKARELLGYRPTHEVSAGLREAM
                     AWYVANAA"
     gene            complement(2204..3226)
                     /gene="galE"
                     /locus_tag="VFV25_04545"
     CDS             complement(2204..3226)
                     /gene="galE"
                     /locus_tag="VFV25_04545"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010809858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEY1392617.1"
                     /translation="MPRILLTGATGYIGSHTWLALLAAGYEVVGIDNFANSSPRVLER
                     LATLAGQAPVFAEADVCDTAALERVLREYAIDAAVHFAAYKAVGESTQKPLEYFRNNL
                     GGLVSLARALGELGGKRLVFSSSATVYGQPERLPITEDALLATTNPYGATKLMGEQIL
                     RELERCDPAWRIAYLRYFNPVGAHESGQIGEDPRGTPNNLMPYVSQVAVGKRPQLQVF
                     GGDYDTVDGTGVRDYIHVMDLAEGHVAALRRLLGGGDSLTVNLGTGTGYSVLQVIRAF
                     EQASGRPVPYQIVARRPGDVAACYADPSRAQQLLGWRATRGLDAMCADAWRWQQGNPG
                     GYESKD"
     gene            3388..3894
                     /locus_tag="VFV25_04550"
     CDS             3388..3894
                     /locus_tag="VFV25_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392618.1"
                     /translation="MLNALPEMFGRAVLDRVTLLINHVLSAEPAAMQKLQPHAGRALD
                     VVWEQWPALLPPPPALSWTVTPAGLLELADAGAADAEGGLRVALSFRDLPGWLASSLQ
                     AGERPPMAIRGDAQFAADINWLADNLRWDIEDDLARLLGDVSAHQLMRVLRGAGALLR
                     GLAGRRGD"
     gene            3946..>4387
                     /locus_tag="VFV25_04555"
     CDS             3946..>4387
                     /locus_tag="VFV25_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEY1392619.1"
                     /translation="MRHLLRLAYIGFIVLRFGLDQLALHSLRKPWLRALARLLAVGRR
                     LDAPRGERLRQALERLGPIFVKFGQVLSTRRDLLPADIADELAKLQDRVPPFPGEVAR
                     RLVEEAYRKPIEAIFERFESEPVASASIAQVHFAVLKGGREVAVK"
BASE COUNT          659 a         1512 c         1532 g          684 t
ORIGIN      
        1 ggcctatctc cggcatgtcg atccgacggg cgagctcagc tccgaggcac tgaaggcggc
       61 cgtgcatctt cggccgacga cggacgcagc agcgctcaag gacgccgact tcatcgtggt
      121 ggccgtgccc acgccggtgg acgatgcgca caaccccgat ttctcgccgc tggtgggcgc
      181 cagcatcgcg gtgggcaaaa acctcaagcg cggcgccacg gtggtgttcg agtccaccgt
      241 ctatcccggc gccaccgagg acgagtgcat cccgctgatc gagcggcact ccggcctcaa
      301 gtggaagcag gacttcttcg tcggctactc gcccgagcgc atcaatcccg gcgacaagga
      361 gcgcacgctg accaggatca caaaggtggt ctcgggcgac acgccacaga ccctggaact
      421 ggtggccgag atctacggca gcatcatcac ggccggcgtg cacaaggcct cgtcgatcaa
      481 ggtggccgag gcggccaagg tgatcgagaa cacgcagcgc gacctcaaca tcgccctcgt
      541 caacgagctg tcgctgatct ttcatcgcat cggcatcgac acgctggaag tgctgcaggc
      601 cgccggcacg aagtggaact tcctgccctt ccggcccggc ctagtgggcg ggcattgcat
      661 cggcgtcgat ccctactacc tcacgcacaa ggccgagcgc ctgggctacc acccccaggt
      721 gatcctggcc gggcggcgca tcaacgacgg catgggcaag tacgtggccg agcagacggt
      781 caagcagatg atccaggccg gcgtctcggt caagggctcg cacgtgatcg tgctgggcct
      841 gaccttcaag gagaactgcc ccgacctgcg caactccaag gtcatcgacg tggtccgcga
      901 gctggagagc tatggcgtga ccgtgcatgt gcacgatccg gtcgcggcgc cggccgaggc
      961 gcgccatgaa tacggcgtcg agctggcgtc ctgggagcac ctcccgcgcg ccaatgccat
     1021 cgtcgccgcc gtctcgcaca aggagctggt ggcgcgtccg ctggacgact atgtcgccaa
     1081 gctcgcgccc ggcggcctct tcgtcgacgt caaatgccag gccgacgcgc aggcgctgcg
     1141 cgcgcgcggc atcaacgtct ggcgcctgtg atgcagctgt cgcccgagct gagcgcggcc
     1201 ctggccgcta gcccgcggcg ctggctggtc accggcgcgg ccggcttcat cggctcgcat
     1261 ctgctggaag tcctgctcgg cgccggccag caggtgacga cgctggacaa cttcgccacc
     1321 ggcaggcagg ccaacctcga cgaggtgaag cgcaaggtgg gcgaagccgc ctgggcgcgc
     1381 caccgcttca tcgagggcga catcgcggac ccggcgatct gccgcagcgc ctgcgagggc
     1441 gtcgagctgg tgctgcacca ggcggcgctt ggctcggtgc cgcgctcgat caaggatccg
     1501 ctggccacgc accgcgccaa cgccaccggc tttctcaaca tgctggtggc cgcgcaaggc
     1561 gagggcgtag cccgcttcgt ctacgcagcg tcgagctcca cctatggcga ttcgccgacg
     1621 ctgcctaagg tcgaggaccg catcggccgc ccgctgtcgc cctatgcggt gaccaaatac
     1681 ctcaacgagc tctatgccga cgtgttcgct cgctgctacg gcgtgagcgc gatcgggctg
     1741 cgctacttca atgtcttcgg cgcgcgccag gatcccaatg gcgcctacgc ggccgtgatc
     1801 ccgcgctggg ccgcgcagat cctgcacggc gaggcctgcg tgatcaacgg cgacggcgag
     1861 acctcgcgcg acttctgcta catcgccaac gcagtgcagg ccaacctgct ggccgcgttg
     1921 accgacaagc ccgaggcgca gaaccaggtc tacaacgtgg ccgtgggcca gcgcaccacg
     1981 ctcaagcagc tgcatgcggc gctcgccgcc gagctgcaga gcctgcgtcc cggcctcgcc
     2041 attgcgccgc cggtgcatgc cgagttccgc tccggcgatg tgcgccattc gctggctgat
     2101 gtgagcaagg cgcgcgagct gcttggctac cgccccacgc atgaagtgag cgccggtttg
     2161 cgcgaggcga tggcctggta tgtcgccaac gccgcctgaa gcctcagtct tttgattcgt
     2221 agccgccggg gttgccctgc tgccagcgcc aggcatcggc gcacatcgcg tccagcccgc
     2281 gcgtggcgcg ccagcccagc agctgctgag cgcggctggg gtcggcatag caggccgcca
     2341 cgtcgcctgg gcggcgcgca acgatctggt agggcacggg ccggccgctg gcctgctcga
     2401 aggcgcgtat cacctgcagc acgctgtagc cggtgccggt gccgaggttc acggtcagcg
     2461 agtcgccgcc gcccagcagc cggcgcagcg cggccacatg gccttcggcc aggtccatca
     2521 cgtggatgta gtcgcgcacg ccggtgccgt ccaccgtgtc gtagtcgccg ccgaagacct
     2581 gcagctgcgg ccgcttgccc accgccacct ggctcacata gggcatcagg ttgttgggcg
     2641 tgccgcgcgg gtcctcgccg atctgcccgc tctcgtgggc gcctacgggg ttgaagtagc
     2701 gcagataggc gatgcgccag gccgggtcgc agcgctccag ctcgcgcagg atctgctcgc
     2761 ccatcagctt ggtcgcgcca taggggttgg tggtggccag cagcgcatcc tcggtgatcg
     2821 gcaggcgctc cggctggcca tagaccgtgg ccgaggagct gaacaccagg cgcttgccgc
     2881 cgagttcgcc cagcgcccgg gccaggctga ccaggccgcc gaggttgttg cggaagtatt
     2941 ccagcggctt ctgcgtcgat tcgccgaccg ccttgtaggc cgcgaaatgc acagcggcat
     3001 cgattgcata ttcgcgcagc acgcgctcga gcgcggccgt gtcgcacaca tcggcctccg
     3061 cgaagaccgg tgcctgcccg gcaagcgtag ccaggcgctc cagcacgcgc ggcgagctgt
     3121 tggcgaagtt gtcgatgccg accacctcat agccggccgc gagcagcgcg agccaggtgt
     3181 gggagccgat atagccggtc gcgccggtga gcaggatgcg gggcatgggg atgcttgggt
     3241 tgagagagtg ggacggcgca gggcgccgcg tcgcgagggc cgtcgaggtg cgagtcggcc
     3301 gatggtctcc attctggcaa acgcggcctc gccccggggc cctcggatgc agggccgcgc
     3361 gcgaaggggc cacctacaat ccttgccatg ttgaacgcct tgccagagat gttcggccgc
     3421 gcagtcctgg accgcgtgac cttgctcatc aaccacgtgc tgagcgcgga gccggccgcg
     3481 atgcagaagc tgcaaccgca cgccggccgc gcgctggacg tggtgtggga gcaatggccc
     3541 gctctgctgc cgccgcctcc ggccttgagc tggaccgtca cgccggccgg cctgctggag
     3601 ctggccgatg ccggcgctgc ggatgcggag ggcggcttgc gggtggcgct cagcttccgc
     3661 gatctgccag gctggctcgc gtcctcgctg caggccggcg agcgtccgcc gatggcgatc
     3721 cggggcgatg cccagttcgc cgccgacatc aactggctgg ccgacaacct gcgctgggac
     3781 attgaagacg acctggcgcg cctgctcggc gatgtgtcgg cgcaccagct gatgcgtgtg
     3841 ctgcgcggcg ccggggcgtt attgcgtggt ctggccggcc ggcgtggcga ctgaggccat
     3901 gcggctttct ttgacgaggc ggccggggcg ctgcaggtgg cgcgcatgag gcatctgctg
     3961 cgcctggcct atatcggctt catcgtgctg cgcttcgggc tggaccagct cgcgctgcac
     4021 agcctgcgca aaccctggct gcgcgcgctg gcgcgcctgc tcgcagtggg gcgccgactc
     4081 gatgcgccgc gcggcgaacg gctgcgccag gcgctggagc ggctggggcc tatcttcgtc
     4141 aagttcggcc aggtgctgtc cacgcggcgc gacctgctgc cggccgacat cgccgacgag
     4201 ctggccaagc tgcaggatcg agtgccaccc ttcccgggcg aggtggcgcg ccgcctcgtc
     4261 gaggaggcct accgcaagcc gatcgaagcg atcttcgagc gcttcgagtc cgaaccggtg
     4321 gccagcgcgt ccattgcgca ggtgcatttc gcggtgctca agggcgggcg cgaggtggcc
     4381 gtcaagg
//
LOCUS       DASUCY010000238         2591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1097328, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000238 DASUCY010000000
VERSION     DASUCY010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097328"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /gene="dctP"
                     /locus_tag="VFV25_04560"
     CDS             <1..520
                     /gene="dctP"
                     /locus_tag="VFV25_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEY1392620.1"
                     /translation="KGLKMRIQSSKVLDAQMRSVGAVPQVLAFSEVYQALQSGVVDGT
                     ENTPSNIYTQKTHEVQKVLTLTHHGYIGYAVVVNKRFWDGLPPDIRTTLEGAMKDATK
                     YADELSKQENDDALEAMRRSGKIQLIEPTAEEKKVLKKAMFAVHREMESRVGRDTEQQ
                     FYKATGFDPKSF"
     gene            522..1880
                     /locus_tag="VFV25_04565"
     CDS             522..1880
                     /locus_tag="VFV25_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEY1392621.1"
                     /translation="MTDRCPEQALAQFAARLDIAQIPPAVLRRAEDLMLDWFGSAQAG
                     KNARPVETLTRFLESMGPGEGPSELLIHRRRSSPLIAAMANAAASHFAEQDDVHNGSV
                     FHPATVVFPAALAVAQALGRSGAELLASVVAGYEVGIRVGEFLGRSHYKVFHTTGTAG
                     TLAAAAAVGRLLRLTPEQMQHALGSAGTQSAGLWEFLRDAADSKQLHVAHAAGAGLSA
                     AYLAQAGFTGARRILEGPQGLAAGTSKDADAARLTDGLGTRWTLAETSFKFHAACRHT
                     HPAADALQLVLRKHALQAGQVRRVTALVHQAAIDVLGPVAAPQTVHQAKFSMGTVLGL
                     IAVHGRAGLAEFDQHYRDAAVVDFRGKVAMQLDAEVDAAYPARWVGKVVVETQDDRRF
                     EGRVDEPKGDPGNTLSRPELEDKALRLAAYQDGASGEEMRRVIARIWSLAQAERVPVF
                     LA"
     gene            1891..>2591
                     /locus_tag="VFV25_04570"
     CDS             1891..>2591
                     /locus_tag="VFV25_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEY1392622.1"
                     /translation="MTARSYLFVPADRPERFDKALAAGADAVILDLEDAVAPEAKDGA
                     RRSLADWLQRASSARVVLRINGADTAWFEDDLALCTAAGSVTTVMLPKAERPEDLARI
                     AAAAPGRALLPLIETAVGFDNMRSLAQAPGVERLVFGSIDFQLDLGIAGEGEELLFFR
                     SQMVLASRLANLAPPVDGVSTAIADVEPLQAEARRARRLGFGAKLCIHPKQVAIVNES
                     FSPSAEELAWAGRVL"
BASE COUNT          420 a          874 c          912 g          385 t
ORIGIN      
        1 caagggcctg aagatgcgca tccagtcgtc caaggtgctc gatgcgcaga tgcgctcggt
       61 cggcgcggtc ccgcaggtgc tggccttttc cgaggtctac caggcgctgc agagcggcgt
      121 ggtcgacggc accgagaaca cgccgtcgaa catctacacg cagaagaccc acgaagtgca
      181 gaaggtcctg acgctcacgc accacggcta catcggctac gcggtggtag tcaacaagag
      241 gttctgggac ggcctgccgc ccgacatccg caccacgctc gaaggcgcga tgaaggacgc
      301 gaccaaatac gccgacgagc tctccaagca ggagaacgat gacgcgctgg aagcgatgcg
      361 caggagcggc aagatccagc tcatcgagcc gaccgccgaa gagaagaagg tcttgaagaa
      421 ggcgatgttt gccgttcatc gcgagatgga gagtcgcgtc ggccgcgaca ccgagcagca
      481 gttctacaag gccaccggct tcgaccccaa gagcttttga gatgaccgac cgttgccccg
      541 aacaagccct ggcgcaattc gccgcgcggc tggacatcgc gcaaattccg cccgccgtgc
      601 tgcgccgcgc ggaggacctg atgctggatt ggttcggctc ggcgcaggcc ggcaagaacg
      661 cgcggccggt cgagacgctc acgcgcttcc tcgagagcat ggggccgggc gagggcccga
      721 gcgaactgct catccaccgc aggcgcagca gtccgctgat cgcggcgatg gctaatgcgg
      781 cggcttcgca tttcgcggag caggacgatg tgcacaacgg ctcggtcttt cacccagcca
      841 ccgtggtctt ccctgcggcg ctcgcggtag cgcaggcgct gggccgcagc ggcgccgagt
      901 tgctggcgag cgtggtggcc ggctatgagg tcggaatccg cgtcggcgaa ttcctcgggc
      961 gctcgcatta caaggtcttc cacaccacgg gcaccgcagg cacccttgcc gcagcggcgg
     1021 cggtagggcg gctgctgcgc ctgacgcccg agcagatgca gcacgccctg ggctcggctg
     1081 gcacgcagtc tgcggggctg tgggagttcc tgcgcgacgc ggccgattcc aagcagctgc
     1141 atgtagcgca tgccgcaggg gcgggcttaa gcgccgccta tctggcccag gccggcttca
     1201 ccggcgcgcg ccgcatcctg gaggggcctc agggtctcgc cgccggcacc tcgaaagacg
     1261 cagacgcggc gcgcctcacc gacggcttgg gcacgcgctg gacgctggcc gagacctcgt
     1321 tcaagttcca cgcggcctgc cgccacaccc acccggcggc cgatgccctg cagctggtgc
     1381 tgcgcaagca cgccctgcaa gcggggcagg tccggcgcgt gacggccctg gtgcaccagg
     1441 ccgcgatcga tgtgctgggc ccggtggccg cgccccagac cgtgcaccag gccaagttct
     1501 cgatgggcac ggtgctgggc ctcatcgcgg tccacggccg tgccggcctg gccgagttcg
     1561 accagcacta tcgcgatgcc gccgtcgtcg actttcgcgg caaggtggcg atgcagctcg
     1621 atgccgaggt cgatgctgcc tatccggcgc gctgggtcgg caaggtggtc gtcgaaacgc
     1681 aagacgatcg ccgcttcgag ggccgcgtcg atgagcccaa gggcgatccg ggcaacacgc
     1741 tgagccggcc cgagctggaa gacaaggcgc tgcgcctggc ggcctatcag gacggcgcca
     1801 gcggcgagga gatgcgccgc gtcattgcgc gcatctggtc gctggcgcag gccgagcgcg
     1861 tgccggtctt cctggcttga ggaggcagac gtgaccgcac gctcctatct cttcgtgccg
     1921 gcggaccgcc ccgagcgctt cgacaaggcc ctggccgccg gcgccgatgc ggtgatcctc
     1981 gacctcgagg atgcagtggc gcccgaggcc aaggacggcg cgcggcgttc gcttgccgat
     2041 tggctgcagc gtgcatcgtc cgcaagggtg gtgctgcgca tcaacggcgc cgacaccgcg
     2101 tggttcgagg acgatctcgc gctatgcacc gccgctggca gcgtgaccac cgtgatgctg
     2161 cccaaggccg agcggcccga ggacctggcg cgcatcgccg ccgcagcgcc gggccgtgcc
     2221 ctgctgccgc tgatcgaaac ggccgtgggc ttcgacaaca tgcgctcgct cgcccaggcg
     2281 ccgggcgtcg agcgactggt gtttggcagc atcgatttcc agctggatct cggcatcgcc
     2341 ggtgagggcg aggagctgct ctttttccgc tcgcagatgg tgctggcctc caggctcgcg
     2401 aacctggcac cgccggtcga cggcgtgagc acggcgattg ccgatgtaga gcccttgcag
     2461 gccgaggccc ggcgcgcgag gcgattgggc ttcggcgcca agctgtgcat acatccgaag
     2521 caggtagcca tcgtcaacga gagcttcagc cccagtgccg aggagctggc ctgggcgggc
     2581 cgcgtgctcg a
//
LOCUS       DASUCY010000239         2383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_551154, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000239 DASUCY010000000
VERSION     DASUCY010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551154"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /gene="hutI"
                     /locus_tag="VFV25_04575"
     CDS             <1..981
                     /gene="hutI"
                     /locus_tag="VFV25_04575"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEY1392623.1"
                     /translation="QRAQEFELRLQGASYEEIARAGGGIRSTVAATRAASDAQLLDSA
                     TRRLQALCAEGLTTIEIKSGYGLSAEHEARCLHIARTLDQQLPVSVRTTYLAAHALPP
                     EFEGRADDYIDAVCAWLPQLHAQGLVDAVDAFCERIGFTPAQTRRVFDAARRLALPVK
                     LHAEQLSDQGGAALAAEFGALSCDHLEYLGDAGVQAMARAGTVAVLLPGAYYFLRETQ
                     LPPVAALRRHGVPIAIASDHNPGSSPALSLLLMLNMACTLFRLTPEEALRGVTVQAAR
                     ALGLADRGRLAAGLRADFAVWEVEHPRELAYYFGHNPCRHVIVGGMERKP"
     gene            978..1778
                     /gene="hutG"
                     /locus_tag="VFV25_04580"
     CDS             978..1778
                     /gene="hutG"
                     /locus_tag="VFV25_04580"
                     /EC_number="3.5.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013339737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050129 - N-formylglutamate deformylase
                     activity [Evidence IEA];
                     GO_process: GO:0019557 - histidine catabolic process to
                     glutamate and formate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate deformylase"
                     /protein_id="HEY1392624.1"
                     /translation="MSDDHVYTLHRGTTPLLLSLPHVGTEIPPELQPAYVPRALVCED
                     ADRHLERLYGFARELGASVLVPRYGRYLIDLNRPPDDTPMYAGANNTELCPSRFFTGE
                     PLYREGRAPDAAEIARRRERYWQPYHAALSGELARLKEAHGHALLWDGHSIKSELPWL
                     FEGKLPDLNLGTAEGRSCAPALREALAQVLASQGDYTQVVDGRFKGGYITRHYGRPQD
                     GIHAVQLEMCWSCYMQETPPYDYLEEKAARVQPLLRRLLETMLTWNPR"
     gene            1763..>2383
                     /locus_tag="VFV25_04585"
     CDS             1763..>2383
                     /locus_tag="VFV25_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY1392625.1"
                     /translation="MEPTMNATPELMWAPQAWLDGRWQSDVLLRLGKGGRWAEIQAGA
                     PCPAAALRLAGPVLPGLVDAHSHAFQRAMAGLAERRERGEDDFWSWRDRMYRVALCIT
                     PDQLRAVAAQLYVELLRGGYTQVCEFHYLHRAPDGSAYKDPLALSWAMADAATEAGLG
                     LTLLPTLYERAGFAQAGLREDQRRFASTSASVLDAARKLNAAKRPLL"
BASE COUNT          364 a          849 c          810 g          360 t
ORIGIN      
        1 cagcgcgcgc aggaattcga gctgcgactg caaggtgcga gctacgagga gatcgcgcgc
       61 gccggtggcg gcatacgctc caccgtggcc gccacgcgtg ctgccagcga tgcgcaactg
      121 ctggattcgg caacgcggcg cctgcaagcc ctgtgtgccg agggcctcac cacgatcgag
      181 atcaagtccg gctatgggct gagcgccgag cacgaggcgc gctgcctgca catcgcgcgc
      241 acactggatc agcagctgcc agtgagcgtg cgcaccacct acctcgccgc ccacgcgctg
      301 ccgccggaat tcgaaggccg cgcggacgac tacatcgacg ccgtctgcgc atggctgccg
      361 cagctgcatg cgcagggttt ggtcgatgcg gtcgatgcct tctgcgagcg cataggcttc
      421 acgcccgcgc agacgcggcg cgtcttcgac gcggcacggc gcctagcgct gcccgtgaag
      481 ctgcatgccg agcagctgag cgaccagggc ggcgcggcgc tggccgccga gttcggcgcg
      541 ttgtcctgcg atcacctgga atacctcggc gacgccggtg tgcaggccat ggcccgcgcc
      601 ggcacggtgg ccgtcctgct gcccggggcc tactactttt tgcgcgagac gcagctcccg
      661 cccgtcgccg cgctgcgccg gcacggcgtg cccatcgcga tcgcgagcga ccacaacccc
      721 ggcagctcgc ccgcgctgtc gctgctgctg atgctcaaca tggcctgcac actgttccgc
      781 ctgacgcccg aagaggcgct gcgcggcgtt acggtgcagg ccgcacgggc gctgggcctt
      841 gccgaccgcg gccggctcgc cgcgggcctg cgcgccgact tcgcggtctg ggaagtcgag
      901 catccgcgcg agctggccta ttacttcggc cacaacccct gccggcacgt catcgtcggc
      961 ggcatggagc gcaagccatg agtgatgacc atgtctatac cctgcaccgc ggcacgacgc
     1021 cgctgctgct gagcctgccc catgtgggca ccgaaatccc gcccgagctg cagcccgcct
     1081 atgtgccgcg cgccctggta tgtgaagacg ccgatcgaca cctggagcgg ctctacggct
     1141 tcgcgcgcga gctgggcgcg agcgtcctcg tgccgcgcta cggccgctat ctgatcgacc
     1201 tgaatcgccc gcccgacgac acgccgatgt atgcgggcgc caacaacacc gagctctgcc
     1261 ccagccgctt cttcacgggc gagccgcttt accgcgaagg ccgggcgccg gatgccgccg
     1321 agatagcgcg gcggcgcgag cgctattggc agccctatca cgcagccttg agcggcgaac
     1381 tggcgcgcct gaaggaagcg catggccacg ccctgctttg ggacgggcac agcatcaagt
     1441 ctgagctgcc ctggctcttc gagggcaagc tgcccgacct caacctcggc acggccgagg
     1501 gcaggagttg tgcacccgca ctgcgcgagg ccttggcgca agtactcgcc agccagggcg
     1561 actacacgca ggtcgtcgac ggccgcttca agggcggcta catcacccgc cattacggcc
     1621 gcccgcagga cggcatccat gcggtgcagc tggagatgtg ctggagctgc tacatgcagg
     1681 agacgccgcc ttacgactat ctggaagaga aggccgctcg ggtacagcct ctgttgcgac
     1741 gtctgctcga aaccatgctc acatggaacc cacgatgaac gctacgcccg agctaatgtg
     1801 ggcgccgcaa gcctggctgg acggccgctg gcagagcgat gtgctgctgc gcctgggcaa
     1861 gggcggccgc tgggccgaga tccaggccgg agcgccctgc cccgccgcgg ctctgcgcct
     1921 ggcaggcccg gtgctgcccg gcctggtcga cgcgcacagc catgccttcc agcgcgcaat
     1981 ggcgggcctg gccgagaggc gcgaacgggg cgaggacgat ttctggtcct ggcgcgaccg
     2041 catgtaccgc gttgcgctgt gcatcacgcc ggaccagctg agggccgtgg cggcccagct
     2101 ctatgtggaa ctgctgcgcg gcggctatac ccaggtctgc gagttccact acctgcaccg
     2161 ggcgcccgac ggctcggctt ataaggatcc ccttgcgctg tcctgggcga tggccgatgc
     2221 ggcgactgag gccggcctgg gcctgaccct gctgccgacg ctgtatgagc gcgccggctt
     2281 cgctcaagcc ggcctgcgcg aggatcaacg ccgcttcgcg agcacttcgg ccagcgtttt
     2341 ggacgcggct cggaagctca acgcagcgaa gcggccgctg ctg
//
LOCUS       DASUCY010000240         3186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_422568, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000240 DASUCY010000000
VERSION     DASUCY010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422568"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="VFV25_04590"
     CDS             <1..870
                     /locus_tag="VFV25_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HEY1392626.1"
                     /translation="ACLRLRMGQDFEAVDPEDDPRFRECWEAYHKLTGREGITPEMAK
                     AALRRSNTLISSMLVKLGYADAMLCGMVGRFDHHLDHVRSVIGAAPGVVVMAAMNALL
                     LDTRTLFLADTFVNAEPDASELAQIALMAAEEIKRFGLPPKVAFLSHSMFGSSTRESA
                     KRVRAAADLFKRLAPAIECDGEMHGDAALSEEVRHRFLPNSSLHGEANLLVLPNIDAA
                     NILFNVLKMTGGHGVTVGPILLGAARPAHILTPSATVRRIINMTALAVADAAAEVTHA
                     RSAFGAPPQGGGR"
     gene            complement(932..>3186)
                     /locus_tag="VFV25_04595"
     CDS             complement(932..>3186)
                     /locus_tag="VFV25_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY1392627.1"
                     /translation="AAAIALAYFCATPSHAQQAPAPEAAASAPSGTTSGAKLETVTVT
                     AERRSESILDVPVSVSTLSGEKLDVLSSGGEDVRFLSGRVPSLNVESSFGRAFPRFYI
                     RGYGNTDFRLNASQPVSLVYDDVVQENPILKGFPVFDLDKIEVLRGPQGTLFGRNTPG
                     GVVKFDSAKPTQRLEGYGSLSYGTYGTTSFEGAVNVPLSQDWAARVSLLAQHRDNWVD
                     NTYQPGPTQHLEGYNDNAARAQLLYAPHKDFSALFNVHARNLDGSSRLFRANIIQPGT
                     NDLVAGFDPSKISIDGKNESLLTSGGANARLRWGLGELSLHSITGYETVHTYNRGDID
                     GGYGAVFAPPSGPGLIPFSSETADGIPEHKQLTQEVRLQSEYAGPLNWQAGLYYFYED
                     YKLESFSYDSLNGGVQNGYERSHQKNTAYALFGALNYAVSPDLKLRGGLRYTYDKKDF
                     GVEDYNNQGFGTPPTMAALAAGGPLEAHFSKSKPSWDISGTYKLKPGANLYARLANGF
                     RAASVQGAGAFNAQSIAEPETNTSIEAGIKADLFDQRARLGFGVFHYVVKDQQLTAVG
                     GANNANILLNAARTVGQGFELDLQAYLTDALLVTLGAGYNDTRIEDPTLAVSTCAACT
                     VTNPRTATGKALIDGNPLPQAPKWTTNFTARYGIPLGKGEAFVYTDWAYRSTVNFFLY
                     ESTEFTGKSLLTGGLRLGYLWGDGKYEAALFGRNITNQIRVVGGIDFNNLTGFINEPR
                     MWGVQFKANL"
BASE COUNT          488 a         1020 c         1113 g          565 t
ORIGIN      
        1 gcctgcctgc ggctcaggat gggccaggac ttcgaggcgg tcgaccccga ggacgatccg
       61 cgcttccggg aatgctggga ggcctatcac aagctgacgg ggcgcgaggg catcacgccg
      121 gagatggcca aggctgcgct acggcgctcc aacacgctga tctccagcat gctggtgaag
      181 ctgggctatg ccgatgcgat gctgtgcggc atggtggggc gcttcgacca tcacctcgac
      241 catgtgcgct cggtgatcgg tgctgccccc ggcgtggtgg tgatggccgc gatgaatgcg
      301 ctgctgctgg acacgcgcac gctgttcctc gccgacacct tcgtcaacgc cgagcccgac
      361 gccagcgagc tggcgcagat cgcgctgatg gccgccgagg agatcaagcg cttcgggctg
      421 ccgcccaagg tcgccttcct gtcgcactcg atgttcggct cgtcgacgcg cgagtcggcc
      481 aagcgcgtgc gcgccgcggc cgatctcttc aagcgcctgg cacccgccat cgaatgcgac
      541 ggcgagatgc acggcgacgc tgcattgtcg gaagaggtgc gccaccgctt tctgcccaac
      601 agctcgctgc acggcgaggc caacctgctg gtgctgccca acatcgacgc cgccaacatc
      661 ctgttcaacg tgctgaagat gacgggcggc cacggcgtca ccgtgggtcc gatcctgctc
      721 ggcgcagcgc ggcctgcgca tatcctgacg ccttccgcga cggtgcgccg catcatcaac
      781 atgacggcgc tggccgtggc tgacgcggcg gctgaagtga cccacgcccg gagcgccttc
      841 ggcgcacccc ctcaaggggg cggccgctag cggcccggca gagccggttc cgcggcgtcc
      901 acttggcaat acctcgctgg tggcggagcg ctcaaaggtt cgccttgaac tgcactcccc
      961 acatgcgcgg ctcgttgatg aagccggtca ggttgttgaa gtcgatgccg ccgaccacgc
     1021 ggatctggtt ggtgatgttg cggccgaaca gcgcggcctc gtacttgccg tcgccccaca
     1081 gatagcccag gcgcaggcca ccggtgagca gcgatttgcc ggtgaactcg gtcgactcgt
     1141 agaggaagaa gttgaccgtg ctgcgatagg cccagtccgt gtagacgaag gcctcgccct
     1201 tgcccagcgg gatgccgtag cgcgccgtga agttggtcgt ccatttgggc gcctgcggca
     1261 gcgggttgcc gtcgatcagc gccttgccgg tcgcggtgcg cgggttggtg acggtgcagg
     1321 ccgcgcaggt cgagaccgcg agggtcgggt cctcgatcct ggtgtcgttg tagcccgcgc
     1381 ccagcgtgac gagcagcgca tcggtcagat aggcctgcag gtcgagctcg aagccctggc
     1441 cgactgtccg cgccgcgttg agcaggatgt tggcgttgtt cgcgccgccc accgcggtga
     1501 gctgctggtc cttcacgacg taatggaaga cgccgaagcc cagccgcgcg cgctggtcga
     1561 acaggtcggc cttgatgccg gcctcgatcg aggtgttggt ctcgggctcg gcgatcgact
     1621 gggcattgaa tgcgcccgca ccctgcacgc tggcggcgcg gaagccgttg gccaggcgcg
     1681 catagaggtt cgcgccgggc ttcagcttgt aggtgccgct gatgtcccag ctcggcttgc
     1741 tcttgctgaa atgagcctcc aacgggccgc cggcggccag tgccgccatc gtgggcggcg
     1801 tgccgaagcc ctggttgttg tagtcctcga ccccgaagtc cttcttgtcg taggtgtagc
     1861 gcaggccgcc gcgcaacttc agatccgggc tcacggcgta gttcagcgca ccgaacaaag
     1921 cataggccgt gttcttttgg tgcgaacgct cgtagccgtt ctgcacgccg ccgttcagcg
     1981 agtcgtagct gaagctctcc agcttgtagt cttcatagaa gtaatacagg ccggcttgcc
     2041 agttgagcgg cccggcgtac tccgactgca ggcggacttc ctgcgtcagc tgcttgtgct
     2101 cgggaatgcc gtcggccgtc tcggacgaga aggggatcag acccggcccc gaaggcggcg
     2161 cgaacacggc gccgtagccg ccgtcgatgt cgccgcggtt gtaggtgtgc accgtttcgt
     2221 agccggtgat cgagtgcagc gacagctcgc ccaagcccca gcgcagccgc gcgttcgcgc
     2281 cgccgctggt gagcagcgac tcgttcttgc cgtcgatcga gatcttgctg ggatcgaagc
     2341 cagccacgag gtcgttggtg ccgggctgga tgatgttggc gcggaacagc cgcgaggagc
     2401 cgtcgaggtt gcgcgcatgc acgttgaaca gcgcgctgaa gtccttgtgc ggcgcataca
     2461 ggagctgcgc ccgcgccgca ttgtcgttat agccttccag gtgctgcgtg gggccgggct
     2521 gataggtgtt gtcgacccag ttgtcgcgat gctgagccag caaggacacg cgcgccgccc
     2581 agtcctgcga caggggcacg ttgacggcgc cttcgaagct ggtggtgcca taggtgccgt
     2641 aggacaggct gccatagccc tcgagcctct gggtgggctt ggccgagtcg aacttcacca
     2701 cgccgcccgg cgtgttgcgg ccgaagagcg tgccctgcgg gccgcgcagc acctcgatct
     2761 tgtcgaggtc gaacaccggg aagcccttga ggatggggtt ctcctgcacc acgtcgtcat
     2821 acaccagcga gaccggttgc gaggcgttca agcggaagtc ggtgttgccg tagccgcgga
     2881 tgtagaagcg cgggaaggca cggccgaagg aggactcgac gttcaggctg ggcacgcggc
     2941 ccgagaggaa gcgcacgtct tcgccgcctg agctcagtac atccagcttc tcgccgctca
     3001 gcgtgctcac cgagaccggc acgtcgagaa tgctctcgct gcggcgctcg gccgtcaccg
     3061 tcacggtctc gagcttggcc cctgaagtgg ttcctgaggg ggcggaggcc gcggcctcag
     3121 gtgccggtgc ttgttgggcg tggctgggcg tggcgcagaa ataggcgagt gcgatggccg
     3181 ctgcca
//
LOCUS       DASUCY010000241         2880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_149506, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000241 DASUCY010000000
VERSION     DASUCY010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149506"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..226
                     /locus_tag="VFV25_04600"
     CDS             92..226
                     /locus_tag="VFV25_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392628.1"
                     /translation="MLSETGFVDIRTSPPFDTFGGARGEAKARKFAVFGYTFLARKPT
                     "
     gene            complement(257..1519)
                     /locus_tag="VFV25_04605"
     CDS             complement(257..1519)
                     /locus_tag="VFV25_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1392629.1"
                     /translation="MQAESQYPFPSSWLARLLLPPGARLDALPLLVARALRGFADGYV
                     AVLLPAYLLGLGLGSLQVGIVATATLLGSALATLAIGVRGHRYRSRALLRGAALLMMA
                     TGLGFAGLSKLWPLVLVAFVGTLNPSSGDVSVFLPLEHARLAAAANGEARTALFARYS
                     VLGAVSAALGALAAAVPDALVGIGGLSRLDALRAMFVLYAVIGLLVWWLYRLPAVETP
                     TTAAAAEATAPLGPSRRVVMRLAALFSVDAFAGGLVVNALLALWLLQRFGLSLAQAGA
                     FFFWAGLLSAASQLLAPAVARRFGLLNTMVFTHIPSSVCLVLAALVPGLVPALALLLM
                     RSALSQMDVPTRTAYVMAVVTPAERPAAASFTAVPRSLAAALSPSISGALLAIGWPAA
                     PLVACGCLKIAYDLVLWRACRKDALGDD"
     gene            complement(1540..2847)
                     /locus_tag="VFV25_04610"
     CDS             complement(1540..2847)
                     /locus_tag="VFV25_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY1392630.1"
                     /translation="MSLFWRTFFLLAVLLTSGVFAWVQTFRALEFEPRAVQAAQQIAS
                     LVNLSRNALQYADKINRVTLVKAISDQEAVKLLPREPKDRYEPFETDRLTRAIGEELR
                     SRLGPTTVVASSVNGQPGLWVGFLIENDQYWLQAERTRVEQVQPSTYFVWVGIALFAT
                     LMGSAAIAGLINRPLQQLSFAASRIREGEFESRLDETMMTREIREVNQGFNRMARELA
                     KVEEDRAVMLAGISHDLRTPLARLRLETEMSVVDDEAKHNMAADIDQLDAIIDKFMDY
                     ARPGEVKLVPIHLISLVEREAAAFRDPNQIRIEVRLPIDTKVMADETELGRVFSNLFE
                     NARRYGRTTETGWARVQVGYARTGPWVIVSVRDHGPGVAQEKLSQLTTPFFRGDAART
                     AATGAGLGLAIVEKTVQRMGGSFDINNATDGGLITHIRLKKAP"
BASE COUNT          474 a          973 c         1039 g          394 t
ORIGIN      
        1 tggcaatcct gtgccggagg ctgcgctggc cgacatagat ctctggactg cgtgaatcgc
       61 cggtggtctt ccgtgtgaag cctggcgaag catgctcagc gaaacggggt ttgtcgacat
      121 ccgcacgagc ccgccgtttg acaccttcgg cggagcgcga ggcgaggcca aggctcggaa
      181 atttgcagtg ttcggctata ccttcctggc gcgcaagccg acttgaattc ggcgcgactc
      241 gagccgcgca ggcgcctcaa tcgtcgccga gagcgtcctt gcggcaggcc cgccacagca
      301 cgaggtcgta ggcgatcttc aggcagccgc aggcaaccag cggcgcagcc ggccagccga
      361 tcgccagcag cgcgccgctg atcgaggggc tgagcgccgc tgccaggctg cgcggcacgg
      421 cggtgaagct ggcggcggcc ggccgctcgg ccggcgtgac gaccgccatc acataggcgg
      481 tgcgcgtggg cacgtccatt tgcgacagcg cgctgcgcat cagcagcagc gccagcgccg
      541 gcacgaggcc aggcaccaag gccgcaagga cgaggcagac gctggacggg atatgcgtga
      601 acaccatggt gttcaagagg ccgaagcgcc gcgccaccgc cggcgcgagc agctgcgagg
      661 ctgcgctgag aagcccggcc cagaagaaga aggcgccggc ctgggccagc gagaggccaa
      721 agcgctgcaa cagccacagc gccagcagcg cgttgacgac caggccgccg gcgaaggcat
      781 cgacgctgaa gagcgcggcc agccgcatca cgacgcggcg cgaagggcct agcggcgcgg
      841 tcgcttccgc ggccgccgcc gttgtcggcg tctccacggc cggcaggcga tacagccacc
      901 agaccagcag tccgatgact gcatagagga cgaacatcgc acgcagcgca tcgagccggc
      961 tcagcccgcc gatgccgacg agcgcatcgg gcacggccgc ggccagtgcg ccgagcgcgg
     1021 cactgacggc gccgagcacg ctgtagcgcg cgaagagcgc ggttcgcgcc tcgccattgg
     1081 cagcggccgc cagccgcgca tgctccagcg gcaggaagac gctcacgtcg cccgaactcg
     1141 gattgagcgt gccgacgaag gcgaccagca ccagcggcca cagcttggag aggccggcaa
     1201 agccgagccc cgtggccatc atcagcagcg ccgcgccgcg cagcagggcg cgcgagcgat
     1261 agcggtgacc ccggacgccg atggccagcg tcgcgagcgc cgagccgagc agggtcgcgg
     1321 tggcgacgat gcccacctgc aggctgccca gccccaggcc cagcagatag gccggcagca
     1381 gcacggccac gtagccgtcg gcaaagccgc gcaaggcgcg cgcgaccagc aggggcaggg
     1441 cgtcgagccg ggcgccgggc ggcagcagca gccgcgccag ccatgagctt gggaatgggt
     1501 actgggattc ggcctgcatg atgcgcgacc gaacaagccc tagggcgcct tcttcaggcg
     1561 tatgtgcgtg atcagcccgc cgtcggtggc gttgttgatg tcgaagctgc cgcccatgcg
     1621 ctgcaccgtc ttttcgacga tggccaggcc caggccggcg ccggtggcgg cggtgcgcgc
     1681 ggcgtcgccg cggaagaagg gcgtggtgag ctgggagagc ttctcctgcg ccacgccggg
     1741 gccatggtcg cgcacgctga cgatcaccca ggggccggtg cgcgcatagc cgacctgcac
     1801 gcgcgcccag cccgtctcgg tggtgcggcc gtagcggcgc gcgttctcga acagattgga
     1861 gaagacgcgg cccagctcgg tctcgtcggc catcaccttg gtgtcgatcg gcagccgcac
     1921 ctcgatgcgg atctggttgg ggtcgcggaa ggccgcggcc tcgcgctcga ccagcgagat
     1981 caggtgtatc ggcaccagct tgacctcgcc ggggcgggcg tagtccatga acttgtcgat
     2041 gatggcgtcg agctggtcga tgtcggccgc catgttgtgc ttggcttcgt cgtcgaccac
     2101 gctcatctcg gtttccaggc gcaggcgcgc cagcggcgtg cgcaggtcgt gcgagatgcc
     2161 ggccagcatc acggcgcggt cttcctccac cttggcgagc tcgcgcgcca tgcggttgaa
     2221 gccctggttc acctcgcgga tctcgcgcgt catcatggtt tcgtccaggc gcgactcgaa
     2281 ctcgccttcg cggatgcggc tggccgcgaa ggacagctgt tgcagcgggc ggttgatcag
     2341 gccggcgatg gcggccgagc ccatcagcgt ggcgaacaag gcgatgccga cccagacgaa
     2401 ataggtcgag ggctgcacct gctcgacccg cgtgcgctcg gcctgcagcc agtactggtc
     2461 gttctcgatc aggaagccga cccacagccc aggctggccg ttgaccgagc tggcaacgac
     2521 cgtggtgggc cccaggcggc tgcgcagctc ctcgccgatg gcgcgcgtca ggcggtcggt
     2581 ctcgaagggc tcgtacctgt ccttgggttc gcgcggcagg agcttcacgg cctcctggtc
     2641 ggaaatagcc ttgaccaggg tcacgcggtt gatcttgtcg gcatattgca gcgcgttgcg
     2701 cgagaggttc accaggctcg caatctgctg ggcggcctgc actgcgcgcg gctcgaactc
     2761 cagcgcgcgg aaggtctgca cccaggcgaa cacgccgctc gttagcagca cggcgaggag
     2821 gaagaacgtg cgccagaaca ggctcaccgc cgcgcgcttc ggcggcttcg cggcaggccg
//
LOCUS       DASUCY010000242         2401 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1466701, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000242 DASUCY010000000
VERSION     DASUCY010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1466701"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VFV25_04615"
     CDS             complement(<1..533)
                     /locus_tag="VFV25_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007839190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HEY1392631.1"
                     /translation="MRKPLLGCIADDFTGATDLANNLVRGGMRVVQTIGVPDTGLDEE
                     ADAIVVALKSRTIAPADAVSQSLQALRWLREQGVAQVYFKYCSTFDSTPAGNIGPVTD
                     ALMRATGCDFTIACPAFPDNGRTVFKGYLYVGDELLHESGMRNHPLTPMTDSNLVRVL
                     QAQTTRKVGLIDYRSVAP"
     gene            complement(578..1240)
                     /locus_tag="VFV25_04620"
     CDS             complement(578..1240)
                     /locus_tag="VFV25_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEY1392632.1"
                     /translation="MIRSIAFIHTVALLVDRFRPRFQAELPQQRCFHMLDESVLQDLL
                     RDGPSPAITRRIVTLAMLAADAGADLIVFTCSSTSPAIDAARKLVPVPIVKIDDAMYA
                     EAARSDGRVGLLCTTRSTLEPSRSLLAEHVAALGTRVEAEPVLVDGAFDALSAGKREE
                     HDALVIEWALALAPRADRLVLAQASLAHLAEPLAQRTGKPVFASPEWMVRDVVARVAA
                     AA"
     gene            complement(1258..2373)
                     /locus_tag="VFV25_04625"
     CDS             complement(1258..2373)
                     /locus_tag="VFV25_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1392633.1"
                     /translation="MLAQLFINACLTGMRMAAPLQSLQLGHGEAAVGCLIALFALSQV
                     FLALPAGRYCERHGLKRPIVMAVAVAVAACAVAAAWPVFPVLCASALLMGGAGGVAII
                     AVQRHAPRIVSEPAALRQAFSWLAIGLALSNVLGPLAAGFIIDGAGFRAVFLGLAALP
                     LLAWLVVGGVMDRASMPAEAANRGGGIWDLWRHAGLRRLLMVTGLLSCCWDVHTFLVP
                     VLGHERGMPASVIGSILGGFGMGAAAVRLLTPLVAAQLREWKILFGAMAVTAVLFAVY
                     PLVHAPLAMGLCSALLGFSLGSTQPMVMSLLHRLAPQHRYGEAVAMRVIVNNASSVGI
                     PTVSGVAGALIGAAGVFWAAGVMVAAGAQLTLRLKEH"
BASE COUNT          441 a          827 c          804 g          329 t
ORIGIN      
        1 ggggcaacgc tccggtaatc aatgaggccc accttgcgcg tcgtctgtgc ctgcagcacg
       61 cgcacgaggt tggagtcggt catcggcgtg agcggatggt tgcgcatgcc cgactcgtga
      121 agcagctcgt cgccgacgta caggtagccc ttaaagacgg ttcggccgtt gtcggggaac
      181 gcggggcagg cgatggtgaa gtcacagccc gttgcgcgca tcaaggcatc cgtgaccggg
      241 ccgatgttgc cggccggcgt ggaatcgaag gttgagcagt acttgaagta gacctgggca
      301 acgccctgct cgcgcagcca tctcagtgcc tgaagcgact gcgagacggc atccgccgga
      361 gcgatcgtgc ggctcttgag cgcaacgaca atggcgtcgg cctcctcgtc tagcccggta
      421 tcgggcaccc caatcgtctg cacgacgcgc atgccgccgc gcacgaggtt gttggccagg
      481 tcggtggcgc ccgtgaagtc gtcggcaatg cagccgagga ggggttttct catgggacgg
      541 actcttctcg gatctcggtg gtgttgcgcg tcgcctctca ggctgcagcg gccacccgag
      601 caacgacgtc gcggaccatc cattcggggc tggcgaacac cggcttgccg gtgcgttgcg
      661 cgagcggttc ggccaagtga gcgagcgagg cctgggccag taccagccgg tcggcacgcg
      721 gcgcgagggc cagtgcccat tcgatcacca gggcatcgtg ctcttcgcgc ttgcccgcgg
      781 acagtgcgtc gaacgcgccg tcgaccagaa cgggctctgc ctccacccgc gtccccaaag
      841 ccgcgacatg ttcggcaagc agcgatcggc tgggctccag ggtggagcgc gtcgtgcaca
      901 gcaggccgac gcggccatca ctgcgagccg cttcagcgta catcgcatcg tcgatcttca
      961 cgatcggtac cggcaccagc ttgcgcgccg cgtcgatcgc gggagaggtc gatgaacagg
     1021 tgaagacgat caggtccgca cccgcatcgg cagccagcat ggccagcgtg acgatgcgcc
     1081 gtgtgatggc aggcgacggc ccgtctcgca gcagatcctg cagcacgctc tcgtcgagca
     1141 tgtggaagca gcgttgctgc ggcagttccg cctggaagcg cggccggaag cggtcgacca
     1201 gcagggctac ggtatggatg aaggcgatgg aacggatcat gttctttcag tgcactttca
     1261 gtgctctttc aggcgcagcg tcagctgggc gcccgcagcc accatcacac cggcggccca
     1321 gaacacacct gcggcgccga tcagcgcgcc cgccacaccc gacaccgtgg gaatgccgac
     1381 gctggacgcg ttgttcacga tcactcgcat cgccaccgct tcgccgtagc ggtgctgcgg
     1441 tgcgagccga tgcagcaggc tcatgaccat gggctgcgtg ctgcccaagc tgaaacccag
     1501 cagtgccgag cacaggccca tggcgagcgg cgcatgcacc agcggataga cggcgaacag
     1561 cactgcggtg accgccatcg cgccgaacag gatcttccat tcgcgcagct gcgccgccac
     1621 gaggggcgtc agcagccgga cggccgcggc gcccatgccg aacccgccca ggatgctgcc
     1681 gatcaccgaa gccggcatgc cgcgctcatg cccgagcacc ggaacgagga acgtgtgcac
     1741 atcccagcag cacgacagca ggccggtcac catcagcaac cggcgcagtc cggcatgacg
     1801 ccagaggtcc cagatgccgc ccccgcgatt tgccgcttcg gccggcatgg acgcacgatc
     1861 catcacgccc ccaaccacca gccaagccag cagcggcaac gcggccagac cgaggaacac
     1921 ggcgcgaaag ccggcgccat cgatgatgaa gccggcggcg agcgggccca gcacattgga
     1981 caaggccagg ccgatggcca gccacgagaa cgcctgccgc aatgcggccg gttcgctcac
     2041 gatccgcggt gcgtggcgct gaaccgcgat gattgcgaca ccgcccgcac cgcccatgag
     2101 aagggcgctg gcgcacagca ccgggaacac cggccacgcc gcagcgacgg cgcaagccgc
     2161 cacggccacg gccaccgcca tcacgatggg ccgcttcagc ccgtggcgct cgcagtaccg
     2221 gcccgcgggc agcgcgagaa acacctgcga aagcgcgaac agcgcgatca ggcatccgac
     2281 cgccgcctcg ccatggccga gctgcaacga ttgcagcggc gcagccatgc gcatgccggt
     2341 caggcaggca ttgatgaaca gctgggcgag caccagccgc ccgagctggc cggctgggcc
     2401 c
//
LOCUS       DASUCY010000243         3598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1145622, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000243 DASUCY010000000
VERSION     DASUCY010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145622"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="VFV25_04630"
     CDS             complement(<1..811)
                     /locus_tag="VFV25_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEY1392634.1"
                     /translation="MRRTRNAKIVATLGPASSDRDTVRRLFLAGVDVFRLNFSHGSAE
                     DHRARFAILRELENETGRPIGILADLQGPKLRVGTFANGPVMLAEGKAFRLDLDPAPG
                     DAQRAGLPHPEIFAALVPGAELLLDDGRLRLVVERCGPEFAETRVLVGGKLSERKGVN
                     VPGVVLPITALTAKDRRDLALALELGADWIALSFVQRPEDVAEARALIAGRAAIVSKL
                     EKPSAVERLDEIVELSDAVMVARGDLGVELPAERVPAIQKRIVRSCRRLGKP"
     gene            complement(819..2114)
                     /locus_tag="VFV25_04635"
     CDS             complement(819..2114)
                     /locus_tag="VFV25_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEY1392635.1"
                     /translation="MTATTSPPQALLRRMFDAAIALAQPSRCVPAHLPASQELGKGRL
                     IVIGAGKASAAMARAVEDHWDGALSGLVVTRYGYAVPCARIEIVEAAHPVPDAAGLAA
                     AERMLKLVQGLSPDDLVLCLISGGGSSLLPLPLEGLTLEHKQAVNRELLKSGATISEM
                     NCVRRHLSAIKGGRLAAACHPARVLTLLISDVPGDNPIDIASGPTVADPTSCEDALVI
                     ARRYGIALPAAVRAVLESGRGESVKPGDARLARAETRMIATPQMALEAAAAVAREAGY
                     APYILGDAIEGEARDVGKVMAGIALQVAERKQPVAAPCVLLSGGETTVTVRGQGRGGR
                     NVEFLLSAAIALNGHARIHGLAGDTDGVDGQEEIAGAYLAPDTLARAWGLGLKPKDSL
                     ADNDGHGFFEALGDSVITGPTLTNVNDFRALLIEPPIEA"
     gene            complement(2144..3049)
                     /locus_tag="VFV25_04640"
     CDS             complement(2144..3049)
                     /locus_tag="VFV25_04640"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0019580 - galactarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HEY1392636.1"
                     /translation="MTTQPLKIGFIGLGIMGAPMAGHLIKAGHQLFVYTLGKMPEAIA
                     KSSATQCTSARGVAERADIIITMVPDTPDVEAALFADNGVAAGLSKGKVVVDMSSISP
                     VETKEFAKKIKALGCDYLDAPVSGGDVGAKNATLSIMVGGEPAVFERVKPVFELMGKN
                     ITLVGGNGDGQTCKVANQIVVALTINAVAEGLLFASRAGADAAKVRQALMGGLATSRI
                     LDILGERMIKRNFDPGFRIALHQKDLNLALGAARQLGLALPATGTAQQLFSACVANGG
                     AAWDHSGLVRALEKLSNFEIGQKAS"
     gene            complement(3140..>3598)
                     /locus_tag="VFV25_04645"
     CDS             complement(3140..>3598)
                     /locus_tag="VFV25_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEY1392637.1"
                     /translation="GREPAGVDRAAAHRSFVDNLRYAAAELKKAGLKLLIEPINTYDI
                     PGFYLNRTAQAAAILDEVGADNAYIQYDIYHAQRMEGELAATVQKYLPRIAHIQLADN
                     PGRNEPGTGEINYAFLFAHLDRIDYQGWIGCEYKPAAATEAGLGWRQRLV"
BASE COUNT          522 a         1220 c         1250 g          606 t
ORIGIN      
        1 ccggcttgcc caggcggcgg cagctgcgca cgatgcgctt ctggatggcc ggcacgcgct
       61 cggccggcaa ctcgacgccg aggtcgccgc gcgccaccat caccgcgtcc gacagctcga
      121 cgatctcgtc caggcgttcc accgccgagg gcttctcgag cttggagacg atggccgcgc
      181 ggccggcgat cagcgcgcgc gcctcggcca cgtcctcggg ccgctgcacg aaggagagcg
      241 cgatccagtc ggcgccgagc tccagcgcca gcgcgaggtc gcgcctgtcc ttggccgtca
      301 gcgccgtgat cggcagcacg acgccgggca cattgacgcc cttgcgctcg gagagctttc
      361 cgccgaccag tacgcgcgtc tcggcgaatt cggggccgca gcgctccacc acgagccgca
      421 gccggccgtc gtcgagcagt agctcggcgc cgggcaccag cgcggcgaag atctcgggat
      481 gcggcaggcc ggcgcgctgc gcgtcgccgg gtgcggggtc cagatcgagg cggaaggcct
      541 tgccctcggc cagcatcacc ggcccgttgg cgaaggtgcc cacgcgcagc ttggggccct
      601 gcaggtcggc caggatgccg atcggccggc cggtttcatt ttccagctcg cgcaggatcg
      661 cgaagcgggc gcggtggtct tccgcgctgc cgtggctgaa gttgagccgg aacacatcga
      721 cgccggccag gaagaggcgg cgcaccgtgt cgcggtccga gctggccggg ccgagcgtgg
      781 cgacgatctt ggcgttgcgg gttctgcgca tgagctggtc acgcctctat tggaggctcg
      841 attaagaggg cccggaaatc gttgacattg gtcagcgtag gcccggtgat caccgagtcg
      901 cccagcgctt cgaagaagcc gtggccgtcg ttgtcggcca ggctgtcctt gggcttgagt
      961 cccaggcccc aagcgcgcgc cagcgtgtcc ggtgcgagat aggcgccggc gatctcctcc
     1021 tggccatcga cgccatcggt gtcgccggcc aagccgtgga tgcgcgcgtg gccgttgagc
     1081 gcaatcgccg ccgacagcag gaattccacg ttgcgcccgc cgcgcccctg gccgcgcacc
     1141 gtcacggtgg tctcgccgcc cgacagcagc acgcaaggcg ccgccaccgg ctgcttgcgc
     1201 tcggccactt gcagcgcgat gccggccatc accttgccga catcgcgcgc ctcgccttcg
     1261 atcgcatcgc ccaggatgta gggcgcatag cccgcctcgc gcgccacggc ggcagccgct
     1321 tccagcgcca tctgcggcgt ggcgatcatg cgcgtttccg cgcgggccag gcgtgcatcg
     1381 ccgggcttga cggattcgcc acggccgctt tcgagcacgg cgcgcaccgc ggccggcagc
     1441 gcgatcccat agcgacgcgc gatgaccagt gcgtcctcgc agctcgtggg atcggccacc
     1501 gtggggccgg aggcgatgtc gatagggttg tcgccaggca cgtcggaaat caagagtgtc
     1561 agcacccgag ccggatggca ggccgcggcc agccgcccgc ccttgatggc cgagaggtgg
     1621 cggcgcacgc agttcatttc gctgatcgtg gcgcccgact tgagcagctc acggttgacg
     1681 gcctgcttgt gttccagcgt caggccctcg agcggcagcg gcagcagcga ggagccgccg
     1741 cccgagatca ggcacagcac gaggtcatcg gggctcagcc cttgcacgag cttgagcatg
     1801 cgctcggcgg cggccagtcc ggccgcatcg ggcaccggat gcgcagcctc gacgatctcg
     1861 atccgcgcgc agggcacggc atagccgtag cgtgtcacga ccagcccgga cagggcgccg
     1921 tcccagtggt cctcgacagc acgcgccatc gcggccgagg ccttgccggc gccgatcacg
     1981 atcaagcgcc ccttgccgag ctcttgcgac gctggcagat gcgccgggac gcagcgcgag
     2041 ggctgggcca acgcgatggc cgcgtcaaac atgcggcgca gcagcgcctg tggcgggcta
     2101 gtggtcgctg tcatcggggg cgttcggtcg tcgtggttgt tgctcagctg gctttctgtc
     2161 caatctcgaa attggacagt ttctccagtg cacgcaccaa gcccgagtgg tcccaggccg
     2221 cgccgccgtt cgcaacgcag gcggagaaca actgctgcgc cgtgccggtg gccggcagcg
     2281 ccagaccgag ctgacgtgcc gcgccgagcg cgaggttgag gtccttctgg tgcagggcga
     2341 tgcggaagcc tggatcgaag ttgcgcttga tcatgcgctc gcccaggatg tcgaggatgc
     2401 gcgaggtggc caggccgccc atcagggcct ggcgcacctt ggccgcgtcg gcgccggctc
     2461 tggacgcgaa cagcaggcct tcggcgaccg cgttgatggt cagtgcgacg acgatctggt
     2521 tggccacctt acaggtctgg ccgtcgccat tgccgccgac cagcgtgatg ttcttaccca
     2581 tcagctcgaa caccggcttg acgcgctcga acacggccgg ctcgccgcca accatgatgg
     2641 acagcgtcgc gttcttcgcg cccacgtcgc cgccgctcac cggcgcatcg agatagtcgc
     2701 agcccagcgc cttgatcttc ttcgcgaact ccttggtctc gaccggcgag atcgagctca
     2761 tgtcgaccac taccttgccc ttgctcaagc cggcggcgac gccgttgtcg gcgaacaggg
     2821 ccgcttcgac atcgggcgtg tcgggcacca tcgtgatgat gatgtcggct cgctcggcca
     2881 cgccgcgcgc gctggtgcat tgcgtcgcac tgctcttggc gatcgcctcg ggcatcttgc
     2941 ccagcgtgta gacgaagagc tggtgcccgg ccttgatgag atggccggcc atgggtgcgc
     3001 ccatgatgcc cagcccgatg aagccgatct tgagaggttg tgtcgtcatt tcattttcct
     3061 agtgactggt gacctgtgac tagtgacccg tgtgaactcg actcagaacc ggtcttctcc
     3121 ggtcactggt cacagttagc taaacaaggc gctgccgcca acccaggccg gcctctgttg
     3181 cagcggcggg cttgtattcg cagccgatcc agccttggta gtcgatgcgg tcgaggtggg
     3241 cgaacaggaa ggcgtagttg atctcgccgg tgcccggctc gttgcggccg gggttgtcgg
     3301 ccagctggat gtgggcgatg cgcggcaggt atttctgcac cgtggcagcc agctcgcctt
     3361 ccatgcgctg ggcgtggtag atgtcgtact ggatgtaggc gttgtcggcc cctacctcgt
     3421 cgaggatggc cgcggcctgg gccgtgcggt tcagatagaa gccggggatg tcgtaggtgt
     3481 tgatcggctc gatcagcagc ttgagcccag ccttcttcag ctcggcggcg gcatagcgca
     3541 agttgtcgac gaagcttcga tgcgccgcgg cgcggtccac gcccgcgggt tccctgcc
//
LOCUS       DASUCY010000244         4253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1097401, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000244 DASUCY010000000
VERSION     DASUCY010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097401"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="VFV25_04650"
     CDS             <1..643
                     /locus_tag="VFV25_04650"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801616.1"
                     /note="catalyzes the formation of L-threonine from
                     O-phospho-L-homoserine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEY1392638.1"
                     /translation="SGNFGNVCAGHVARMMGLPIGRLVVATNENDVLDEFFRTGSYRV
                     RGATETHETSSPSMDISKASNFERFVFDLLGRDAARTRQLFKTEIDQGGSFSLAPAEF
                     ARVGEFGFASGKSTHADRLATIRDTWQRFGVMIDTHTADGLKVARERASAGVPMLVLE
                     TALPVKFADTIREALGREPERPAALQGLEQRPRRFKVMPADAGAVKDYIAQNV"
     gene            680..1204
                     /gene="mobB"
                     /locus_tag="VFV25_04655"
     CDS             680..1204
                     /gene="mobB"
                     /locus_tag="VFV25_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HEY1392639.1"
                     /translation="MKVVGFCGYSGAGKTTLVEQLIPRLRAAGQRVSVVKHAHHAFDV
                     DRPGKDSYRHRAAGAFEVLVASSRRLAKMREYEVEAEPTAHQLLAELAECDWVLVEGF
                     KHADLPKIEVWRAANDKPALYADDAFVTAIATDAPERLPQPTDLPLLPLNDPDAIAQF
                     LMSQASRYEYRRPA"
     gene            1182..2417
                     /gene="glp"
                     /locus_tag="VFV25_04660"
     CDS             1182..2417
                     /gene="glp"
                     /locus_tag="VFV25_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEY1392640.1"
                     /translation="MNTAAPPKPMLGLDEAVQRLVAATVAIGETQQLPTLDALGRVLA
                     VDLASPLDVPPLDNSAMDGYALRAADVPQAGVVLPISQRIAAGQVGQPLAPGSAARIF
                     TGAPVPEGADAVVMQELCESAGDGVRVNAVPRAGQAIRRRGEDVSCGAVVLRRGTRLT
                     PQALGLAATIGTASLSVVRRPRVALFSTGDELVMPGGPLRAGAIYNSNRYMLRGLLQA
                     LGCEVSDLDIVPDRLDATREALRSAARGHDLIVSSGGVSVGEEDHLKPAVQTEGRLDL
                     WQIAIKPGKPLAFGEVYNQESRAALFIGLPGNPVSSFVTFVLAVRPVLQALQGMAHQP
                     PRGLPLRADFDWPKADSRREFLRVRRNADGGLELYPNQGSGVLTSVAWSDGLVDNPPG
                     QVIRAGDVVRFLPYAELLA"
     gene            2414..2668
                     /gene="moaD"
                     /locus_tag="VFV25_04665"
     CDS             2414..2668
                     /gene="moaD"
                     /locus_tag="VFV25_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HEY1392641.1"
                     /translation="MKLTVRYFASIREALGSGETLELPGPATLGELRDLLIARGGRHA
                     EALARGSALRAAVNQVMATDESLPLAEGTEVAFFPPVTGG"
     gene            2682..3170
                     /locus_tag="VFV25_04670"
     CDS             2682..3170
                     /locus_tag="VFV25_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEY1392642.1"
                     /translation="MSEPRVRIQPEDFDLAAELAALRAGDLDVGAVASFVGTVRGGSV
                     EGGGEGVSLMELEHYPGMTERAIEAMMAEAQRRFDIRGVRVIHRVGPLRPGEQIVLVA
                     VTSRHRGQAFLACEFLMDYLKTQAPFWKKEHTPQGARWVDARVADDEALKRWGIAAGN
                     AG"
     gene            complement(3191..>4253)
                     /locus_tag="VFV25_04675"
     CDS             complement(3191..>4253)
                     /locus_tag="VFV25_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1392643.1"
                     /translation="LVTSLHASAFAVGLIEGTAEATALIVKVFSGVLSDYWGKRKPLA
                     LLGYGLGAASKPLFALAGSVGLVFGARMIDRVGKGIRGAPRDALIADIAPPSLRGAAF
                     GLRQALDTAGAAIGPLLAMVLMLAWADDFRRVFWVAVLPAALAVALLAFGVQEPEAAA
                     QLRRGWPIRRASLKRLPAAYWQIVLIGAIFTLARFSEAFLLLRARQGGLPLAYTPLVL
                     VAMNAVYTLTAYPFGRLADTMSHHRLLAWGLAVLIAADISLAFDGPWVWIGILLWGLH
                     MGMTQGLLAAMIAHAAPPELRGTAYGFFNLVSGIAMLAASALAGLLWDRYGAAQTFIA
                     GALFSALALAVLVLRKRPA"
BASE COUNT          672 a         1459 c         1513 g          609 t
ORIGIN      
        1 ctcgggcaac ttcggcaatg tctgcgccgg ccacgtggcg cgcatgatgg gcctgcccat
       61 cggccggctg gtggtcgcga ccaacgagaa cgacgtgctc gacgagttct tccgcaccgg
      121 cagctaccgc gtgcgcggcg cgaccgagac gcacgagact tcgagcccgt cgatggacat
      181 ctccaaggcc tcgaacttcg agcgcttcgt cttcgacctg ctgggccgcg atgccgcccg
      241 cactcggcag ctgttcaaga ccgagatcga ccagggcggc agcttcagcc ttgcaccggc
      301 cgagttcgcg cgcgtaggcg agttcggctt cgcctcgggc aagagcacgc atgccgaccg
      361 cctggcaacc atccgcgata cctggcagcg cttcggcgta atgatcgaca cccacaccgc
      421 cgacggcctc aaggtggcgc gcgagcgggc cagtgccggc gtgcccatgc tggtgctgga
      481 aacggcgctg cccgtcaagt tcgccgacac catccgcgag gccctgggtc gcgaacccga
      541 gcggcccgcc gcgctgcagg gcctggagca gcgcccgcgc cgcttcaagg tgatgccggc
      601 ggatgccggt gcggtcaagg actacatcgc gcagaatgtc tgagcacgct ggcggagggc
      661 cgccggcgca aggcaaggca tgaaggtcgt cggtttctgc ggatattcgg gtgcgggcaa
      721 gaccacgctg gttgagcagc tgatcccgcg cctgcgcgcg gccggccagc gcgtgtcggt
      781 cgtcaagcac gcccaccacg ccttcgacgt cgatcgtccc ggcaaggact cctaccgcca
      841 ccgcgccgcc ggtgccttcg aggtgctggt ggcctccagc cggcgcctgg cgaagatgcg
      901 cgaatatgag gtcgaagccg aaccaacggc gcaccagctg ttggccgagc tggccgagtg
      961 cgactgggtg ctggtcgagg gcttcaagca cgccgatctg cccaagatcg aggtctggcg
     1021 cgcggccaac gacaagccgg ccctgtatgc ggacgatgcc ttcgtcactg ccatcgcgac
     1081 cgatgcgccg gaacgattac cgcagccgac cgatctgccg ctgctgcccc tcaacgaccc
     1141 tgatgccatt gcgcagttcc tgatgagtca agcttcgcgc tatgaatacc gccgccccgc
     1201 ctaagcccat gctcggtctc gacgaggccg ttcaacgcct ggtggcggcg actgtggcca
     1261 tcggggaaac gcagcagctg cccacgctgg acgcgctggg ccgcgtgctg gccgtcgacc
     1321 tggcctcgcc gctggacgtg ccgccgctgg acaacagcgc gatggacggc tacgccctgc
     1381 gggcagccga tgtgccgcag gccggcgtgg tcttgccgat cagccagcgc atcgccgcgg
     1441 gccaggtcgg gcagccgctg gctcccggca gcgccgcgcg cattttcacc ggcgcccccg
     1501 tgcctgaggg agccgatgcg gtggtgatgc aggagttgtg cgaaagcgcg ggcgacggcg
     1561 ttcgcgtcaa cgccgtgccg cgggccggcc aggcgatccg gcggcgtggc gaggacgtga
     1621 gctgcggcgc ggtcgtgttg cgtcggggca cccggctcac accccaggcg ctgggccttg
     1681 ccgcaacgat aggcaccgcc agcctgagcg tggtgcgccg gccgcgcgtg gcgctgtttt
     1741 ccaccggcga tgagctggtg atgcccggcg ggccgctgcg ggcgggggcg atctacaact
     1801 ccaaccgcta catgctgcgc ggcctgttgc aggccctggg ctgcgaggtc agcgatctgg
     1861 acatcgtgcc agatcggctc gacgcgacgc gcgaggcctt gcgcagcgcg gcgcgtgggc
     1921 acgatctcat cgtcagctcg ggcggcgtct cggtcggcga agaagaccat ctcaagcccg
     1981 cggtgcagac agaagggcgg ctcgacctgt ggcagatcgc gatcaagcca ggcaagcccc
     2041 tggcctttgg cgaggtctac aaccaggaaa gccgtgccgc gctcttcatc ggcctgccgg
     2101 gcaatccggt gtcgagcttc gtcacctttg tgctcgccgt tcggcccgtg ctgcaggccc
     2161 tgcagggcat ggcccaccag ccgccgcgcg gcttgccgct gcgcgcggat ttcgactggc
     2221 ccaaggccga cagccgccga gagttcctgc gcgtcaggcg caatgccgac ggcgggctgg
     2281 agctctatcc caaccagggc tcgggcgtgc tgacttcggt ggcctggtcc gatgggctgg
     2341 tggacaaccc gccgggccaa gtcatacgcg ccggcgatgt ggtgcgcttc ctgccttatg
     2401 cggagctgct ggcatgaagc tcacggtgcg ctatttcgcg tcgatacgcg aggccctggg
     2461 cagcggcgaa acgctggagc tgcccggccc cgccacgctg ggcgagctgc gcgatctgct
     2521 catcgcccgc ggtgggcggc atgccgaggc gctggcccgc gggagcgcct tgcgcgccgc
     2581 cgtgaaccag gtcatggcga cggatgaaag cctgccgctg gccgaaggga cggaagtggc
     2641 attctttcct ccggtcaccg gaggctgaga ccagccccat catgagcgaa ccccgcgtgc
     2701 gcatccagcc ggaagacttc gacctggccg ccgaactggc ggccctgcgc gccggcgatc
     2761 tggacgtcgg cgcagtggcg agctttgtcg gcacggtgcg tggcggcagt gttgaaggcg
     2821 ggggcgaggg cgtgagcctg atggagctgg agcattaccc cggcatgacc gagcgcgcta
     2881 tcgaggcgat gatggccgag gcgcagcgcc gcttcgacat ccgcggtgtg cgcgtcatcc
     2941 atcgcgtcgg ccccctgcgc ccaggcgagc agatcgtgct ggtggccgtg acttccaggc
     3001 accgcggcca ggcctttctg gcctgtgagt tcctgatgga ctatctcaag acccaggcgc
     3061 cgttctggaa gaaggagcac acgccgcaag gcgcgcgctg ggtcgacgca agggtggccg
     3121 acgacgaggc gctcaagcgc tggggcatcg cggccggtaa cgccggctga gccctgcagg
     3181 ctcttgcaga tcaggccggc cgcttcctca ggacgaggac tgccagggcc agggcgctga
     3241 acagggcgcc cgcgatgaag gtctgcgccg cgccgtagcg gtcccagagc agcccggcga
     3301 gagcgctggc cgccagcatc gcgatgccgc tgaccaggtt gaagaagccg taggccgtgc
     3361 cgcgcagttc gggcggcgcg gcgtgcgcga tcatggccgc gagcaggccc tgggtcatgc
     3421 ccatgtgcag cccccacagc aggatgccga tccagaccca ggggccgtcg aaagccagag
     3481 aaatatcggc cgcgatcagc accgcgagcc cccaggccag caggcgatga tggctcatgg
     3541 tgtcggccag cctgccgaag gggtaggcgg tcagcgtgta gacggcgttc atggccacca
     3601 gcaccaaggg cgtgtaggcc agcggcagac cgccctggcg ggcacgcagc aacagaaagg
     3661 cctcgctgaa tcgagccagc gtgaagatgg cgccgatcag cacgatctgc cagtaggcgg
     3721 cgggcaggcg tttgaggctt gcgcggcgta tcggccagcc gcggcgaagc tgtgccgcgg
     3781 cttcgggctc ctgcacgccg aaggccagca gggctacggc cagcgcggca ggcaagaccg
     3841 cgacccagaa cacgcggcgg aagtcgtcgg cccaggccag catcaggacc atcgcgagca
     3901 agggcccgat cgccgcgccg gccgtgtcca gcgcctggcg cagcccgaag gccgcgccgc
     3961 gcaaagaggg cggcgcgatg tcggcgatca gcgcatcgcg tggcgccccg cgtatgccct
     4021 tgccgacgcg gtcgatcatc ctggcgccga ataccaggcc cacgctgccg gccagtgcaa
     4081 acaaaggctt ggacgccgcc cccagcccgt agcccagcaa ggccagcggc ttgcgcttgc
     4141 cccagtagtc gctcagcacg cccgagaaga ctttgacgat gagggccgtc gcctcggccg
     4201 tcccctcgat cagcccgacc gcgaaggcgc tggcatggag gctcgtgacc agg
//
LOCUS       DASUCY010000245         3324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1274109, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000245 DASUCY010000000
VERSION     DASUCY010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1274109"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="VFV25_04680"
     CDS             <1..613
                     /locus_tag="VFV25_04680"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEY1392644.1"
                     /translation="VTLDKTRVSGSCFAPSVEWHVAGPRYHRTMPASPAKRRHQSAVE
                     TPRDASMLANEVRVLRRFRIVFNAIKAHFQQVEKQAGVGGAQLWALSVIRQRPGIGVN
                     ELARAMDVHQSTASNLVKTLAERNLIVATKEGSDRRAVQLRLLASGAEVLDRAPGPFT
                     GVLANALAALDADTLARLDEDLGKLISALDADERAAGIPLAQM"
     gene            complement(712..1245)
                     /gene="pal"
                     /locus_tag="VFV25_04685"
     CDS             complement(712..1245)
                     /gene="pal"
                     /locus_tag="VFV25_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEY1392645.1"
                     /translation="MRSAKSGLILASLTALLAACSSTPVAPPPAPVATAAPAPAPAPA
                     PAPQSQVTTVNLPDYLDPNNPLSKERSVFFDFDDFSVKQQYTPLLQRHGNYLSSHAAV
                     PVRVEGNADERGSSEYNLALGQKRAQAVVNALKIYGVKDSQLEAISYGKERPKATGHD
                     EAAWAENRRADLVYPNK"
     gene            complement(1407..2306)
                     /locus_tag="VFV25_04690"
     CDS             complement(1407..2306)
                     /locus_tag="VFV25_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY1392646.1"
                     /translation="MRVTDLGELLLLAALWGASFLFMRLGAGELGPIALASLRVTLAT
                     VCLLPVLWRRGQMDVLRRHWKPIFIVGITNSALPFLCFGYAALSITAGLASIFNATSP
                     LWGAVIGWLWLRDRLTPERVLGLVIGFGGVLWLAWDKASFKPGADAAGWAVLACLAAT
                     LFYGLSANYTKRHLAGVAPLAVATGSQLSAALVLALPAAWAWPAAPVSLKSWGAVLLL
                     AVLCTAIAYVLYFRLIARIGPANAIAVTFLIPVFAVFWGWVALSEVLTSNMAIGCAVI
                     LLGTSLSTGFWKPRLARRGVADA"
     gene            complement(2303..2806)
                     /locus_tag="VFV25_04695"
     CDS             complement(2303..2806)
                     /locus_tag="VFV25_04695"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEY1392647.1"
                     /translation="MKSQARYIAQTTIETPLGPLLLARSEQGLAGAWFEGQKDHPGPF
                     ADTPERPDDPLLKEVGRQFADYWAGKRTSFDLPLDLQQGTPFQRAVWRALLGVGTGQT
                     LSYGEIAERIGSPRAVRAVGAAVGRNPVSVIVPCHRIIGRDGSLTGYAGGLHRKTALL
                     KLEGVLA"
     gene            complement(2809..>3324)
                     /locus_tag="VFV25_04700"
     CDS             complement(2809..>3324)
                     /locus_tag="VFV25_04700"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEY1392648.1"
                     /translation="ELAVRAVLGQRVTVAAARTLARRLVERFGEHLASSSPWPELHLS
                     FPSPAILAQADPQHIGELGILRSQTQAIQALAKLWQDEAAHGLTLLPEELIRRLVDIP
                     GLGPWTAHYIAMRTLAWPDAFPPGDVAVLKAMGLSKGKAAEREAEARSQAWRPWRAYA
                     VLRLWQSLENP"
BASE COUNT          577 a         1089 c         1150 g          508 t
ORIGIN      
        1 ggtaacgctt gacaaaactc gcgtttctgg tagctgtttt gccccttcgg tcgagtggca
       61 cgttgcgggg ccccgctatc atcggaccat gccagccagc cccgccaaac gacggcatca
      121 gagcgcagtg gaaaccccgc gcgacgcaag catgttggcc aacgaggtgc gggtgctgcg
      181 gcgcttccgg atcgtcttca acgcgatcaa ggcccacttc cagcaggtcg agaagcaggc
      241 cggagtcggc ggtgcccagc tgtgggcgct cagcgtgatc aggcaaaggc cggggatcgg
      301 cgtcaacgag ctggcgcgtg cgatggacgt gcaccaatcc accgccagca atctggtgaa
      361 gacgctggcc gagcgcaatc tgatcgtggc gaccaaggag ggcagcgacc gccgcgcggt
      421 ccagctgcgg ctgctggcct cgggtgccga ggtgctggac cgcgcgcccg ggcccttcac
      481 cggcgtcctg gccaatgcgc tcgccgcgct ggatgccgat acgctcgccc gcctcgacga
      541 ggatctcggc aagctgatct cggctctgga cgcggacgag cgggcggccg gcattccgct
      601 cgcgcagatg tagccgactg cggaacccac aaacgaaacg ggcagatgac cggggccaag
      661 ccccagccaa ctgcccgtat ctgcccgcag gctttggtct aagcggcgcg cttacttgtt
      721 cggatagacg aggtccgcgc gacggttctc agcccaggct gcttcgtcat ggccggtggc
      781 cttgggacgc tccttgccgt agctgatcgc ctcgagctgc gagtccttca cgccatagat
      841 cttgagcgca ttgaccacgg cttgggcgcg cttctgcccc agggcgaggt tgtactcgga
      901 gctgccgcgc tcgtcggcat tgccttccac gcgcaccggc acggcggcgt gcgaagacag
      961 gtagttgcca tggcgctgca gcagcggggt gtattgctgc ttcacgctga agtcgtcgaa
     1021 gtcgaagaag acgctgcgct ccttggacag cggattattc ggatcgaggt agtctggcag
     1081 gttgaccgtg gtcacctggg attgcggcgc cggggccggt gcgggggcgg gcgcaggggc
     1141 ggccgtcgcg acaggggccg gcgggggagc gacgggagtg ctgctgcagg cggcgagcag
     1201 cgcagtcagg gacgccagga tcaggccgga ttttgcggat ctcatacgtt gttctccaga
     1261 taagcaaatc gccggctcgg caacaggctg cgagctcggg gcgctgcgca ccgtgtagcg
     1321 gccgcagtat ctggagttta cattcgcatt tgtgcaaata tatataaggc taggttaagg
     1381 cggcctcttg caggcttgac gcaacatcag gcgtcggcaa cgccgcgcct tgccagccgc
     1441 ggcttccaga agccggtcga caggctcgtt cctagcagga tcacggcgca gccgatcgcc
     1501 atgttcgacg tcaatacctc gctcagggcg acccagcccc agaacacggc gaacaccggg
     1561 atcaggaagg tcacggcgat tgcgttggcc ggtccgatgc gggcgatgag gcggaaatac
     1621 agcacgtagg cgatcgccgt gcatagcacc gcgagcagca gcacggctcc ccaggacttc
     1681 agcgataccg gagcggctgg ccatgcccag gcggccggca gggcgagcac cagcgcggcc
     1741 gacagctggc tgcccgtcgc aacggccaga ggcgcaacgc cggcgaggtg gcgcttggtg
     1801 tagttggccg agaggccgta gaacagcgtc gccgccaggc aggcgagcac ggcccagccg
     1861 gccgcatccg ctccgggctt gaagctggcc ttgtcccagg ccagccagag cacgccgccg
     1921 aagccgatca caaggcccag cacgcgctcc ggtgtaagcc tgtcgcgcag ccagagccag
     1981 ccgatcacgg cgccccacag cggcgaggtc gcgttgaaga tggatgcgag cccggccgtg
     2041 atgctcagcg ccgcatagcc gaagcacagg aagggcaggg ccgagttggt gatgccgacg
     2101 atgaagatcg gcttccagtg cctgcgcagc acgtccatct gcccgcgacg ccataacacg
     2161 ggcagcaggc agacggtggc cagcgtgacg cgcagcgatg ccagggcgat cggcccgagc
     2221 tcgccggcgc ccaggcgcat gaagagaaaa gaggcgcccc acagcgccgc gagcagcagc
     2281 agctcgccca ggtctgtaac cctcatgcaa gcactccttc gagtttcaac agcgccgtct
     2341 tgcgatgcag gccgccggca tagccggtga gcgagccgtc gcggccgatg atgcggtggc
     2401 agggcacgat caccgacacc ggattgcggc ccaccgccgc cccgactgcg cgcactgctc
     2461 gcggcgagcc gatccgctcg gcgatctcgc cgtagctcaa ggtctggccg gtgccgacgc
     2521 ccagcagcgc ccgccacacg gcgcgctgga agggcgtgcc ctgctgcaga tcgagcggga
     2581 ggtcaaaact cgtgcgcttg cccgcccaat agtcggcgaa ctgcctgcca acctccttga
     2641 gcagcgggtc atccggccgt tcgggtgtgt ccgcgaaggg accaggatga tccttctgcc
     2701 cctcgaacca cgcgccggcc agaccctgtt cgctgcgggc gagcagcaac gggccaagtg
     2761 gcgtctctat cgtggtctgg gcgatgtatc gagcttgcga tttcatgatc agggattctc
     2821 cagtgattgc cacagccgca gcactgcata ggcccgccag gggcgccagg cctgggagcg
     2881 agcctcggcc tcgcgctcgg cggccttgcc cttggacagc cccatggcct tcagcacggc
     2941 cacgtcgccg ggcggaaagg catcgggcca ggccagggtg cgcatcgcga tgtagtgagc
     3001 tgtccagggg ccgaggccgg ggatgtcgac gagcctgcgt atcagttctt cgggcagcag
     3061 cgtgaggccg tgggccgcct cgtcctgcca cagctttgcc agcgcttgta tcgcctgagt
     3121 ctgactgcgc aggatgccga gttcgccgat gtgttgcggg tccgcctggg caaggatggc
     3181 gggcgaagga aagctcaaat gcagctcggg ccagggtgaa ctcgaagcca gatgctcccc
     3241 gaagcgctcc acgagccgcc gtgccagcgt tcgcgccgcc gcgaccgtga cgcgctggcc
     3301 cagcaccgcg cgcactgcaa gctc
//
LOCUS       DASUCY010000246         2446 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_37108, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000246 DASUCY010000000
VERSION     DASUCY010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37108"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="VFV25_04705"
     CDS             <1..329
                     /locus_tag="VFV25_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392649.1"
                     /translation="GGELWPGALVAVRVLLRTEPDAIVVPATAVQQAQQGSFVYVVKP
                     DETVERRAVDVGATVGAQQWVRKGLVAGETVVTQGQFRLAPGLKVARLTAAAPAASSA
                     DAGVVQ"
     gene            326..>2446
                     /locus_tag="VFV25_04710"
     CDS             326..>2446
                     /locus_tag="VFV25_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018423302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY1392650.1"
                     /translation="MTLSEPFIRRPVATILLALGLLLLGGVSYFKLPVAPLPQVDFPT
                     ISVSASLPGASAETMATSVATPLERAFSLVPQVTAMTSSSSQGTTSITLQFDLARNID
                     AAAQDVQQAINAASGLLPKNLPSAPSFHKVNPAEFTVMSIALSSGTLPLTELDRYADN
                     YIAQQISQMPGVGLVDFHGEQRPALRVQVDPDALAARGLTLDDVRSVIGLSTVNSPKG
                     SIDGGSRSVTLNTTDQMMHAEQYRSMVVAYKNGLPIRVSDIGNVVSAAEDSRQLAVLQ
                     GKPAIIVDIHKQPGFNVIETTRAIADRLPALTASLPRDVKVEVVGDRTQTIDASVHDV
                     QFTLMLSIGLVVLVIFAFLRNLSATLIPSITIPLSLVGTFGVMYLLGYSLDNLSIMGL
                     SIAVGFVVDDAIVVMENIVRHLEEGKPPMQAAIVGTREVGFTIVSMTISLIAVFIPIL
                     LMGGVIGRLFREFAVTVSVAIIVSGVISLTVTPMMCAWRLKHTQPAQHGRVYRWIESV
                     FDAMLLGYQRSLDWALDRPRLMLAVTLATLAATLALYVWVPKGFFPQQDTGLIIGSAE
                     AAPDVTFSAMSERMAALSEIVQTDPDVANVYYWIGPNPTLSQGRLLINLKPFGERSSS
                     AEQIVARLKPRVASVQGIALYMQLRQDIQVGGRPSKTQFQYTLQAADIGELNHWSGVL
                     QQRLKTLPQLKDVTSDQQQSAPEAT"
BASE COUNT          395 a          854 c          805 g          392 t
ORIGIN      
        1 ccggcggcga gctgtggccc ggcgcgctgg tggccgtgcg cgtgctgctg cgcaccgagc
       61 ccgatgccat cgtggtgccc gccaccgccg tgcagcaggc ccagcagggc agcttcgtct
      121 acgtggtcaa gccggacgag acggtcgagc ggcgtgcggt ggacgtgggt gccacggtcg
      181 gcgctcagca gtgggttcgc aagggtctgg tggcggggga gaccgttgtc acgcagggcc
      241 agttccggct ggcgccgggg ctcaaggtgg cgcggctgac cgctgccgca cccgcggcca
      301 gctcggcgga cgcaggggtc gtgcaatgac gctgtccgag cccttcatcc ggcgccccgt
      361 cgcgacgatt cttctggcgc tgggcctgct gctgctcggc ggcgtctcgt atttcaagtt
      421 gccggtggca ccgctgcctc aggtggactt cccgaccatc tccgtatccg cctcgctgcc
      481 cggcgccagc gccgagacca tggccacctc ggtggcaaca ccgctggagc gcgccttctc
      541 gctggtcccg caggtcacgg ctatgacctc gtcgagctcg cagggcacga cctcgatcac
      601 gctgcaattc gacctcgcgc gcaacatcga cgccgccgct caggacgtgc agcaagccat
      661 caacgcggcc agcggcctgc tgcccaagaa cctgccgagc gcgccgagct tccacaaggt
      721 caatccggcc gaattcacgg tcatgtcgat cgcgctcagc tccggcacgc tgccgctgac
      781 cgagctggac cgctacgccg acaactacat cgcgcagcag atctcgcaga tgccgggcgt
      841 cgggctggtg gatttccacg gcgagcagcg ccctgcgctg cgcgtgcagg tcgaccccga
      901 cgccctggcc gcacgcggcc tgacgctgga cgacgtgcgt agcgtcatcg gcttgagcac
      961 cgtcaacagc cccaagggca gcatagacgg cggcagccgc tccgtgacgc tgaacacgac
     1021 cgaccagatg atgcacgccg agcagtaccg ctcgatggtg gtcgcctaca agaacggcct
     1081 gccgatccgc gtcagcgaca tcggcaacgt cgtcagcgca gcggaggaca gcaggcagct
     1141 ggccgtgctg caaggcaagc ccgcgatcat cgtggacatc cacaagcagc cgggcttcaa
     1201 cgtgatcgag accacgcggg ccatcgccga ccgcctgccg gcgctcacgg cctcgctgcc
     1261 gcgcgatgtg aaggtcgagg tggtgggcga tcgcacgcag accatcgacg cctcggtgca
     1321 cgacgtgcag ttcacgctga tgctgtcaat cggactggtc gtcctcgtga tcttcgcctt
     1381 cctgcgcaac ctgtccgcga ccctcatccc cagcatcacg atcccgctgt cgctggtggg
     1441 caccttcggc gtcatgtacc tgctcggcta cagcctggac aacctgtcca tcatgggctt
     1501 gagcatcgcg gtgggcttcg tggtggacga cgccatcgtc gtgatggaaa acatcgtgcg
     1561 ccacttggag gagggcaagc cgccgatgca ggccgccatc gtcggcacgc gcgaggtcgg
     1621 cttcacgatc gtctcgatga cgatctctct gatcgccgtc ttcatcccca tcctgctgat
     1681 gggcggcgtg atcgggcgac tgttccgcga gttcgcggtc acggtgagcg tggccatcat
     1741 cgtgtcgggc gtgatctcgc tcaccgtcac gcccatgatg tgcgcctggc gcctgaagca
     1801 cacccagccc gcgcagcacg gccgggtcta ccgctggatc gagagcgtgt tcgacgccat
     1861 gctgttggga taccaacgca gcctggactg ggcgctggac cggccgcgcc tgatgctggc
     1921 cgtgacactg gccacgctgg cggccacgct ggcgctctat gtctgggtgc ccaagggctt
     1981 cttcccccaa caagacactg gcctgatcat cggcagtgcc gaggccgcgc ccgacgtcac
     2041 cttctcggcg atgagcgaac gcatggcggc gctgagcgag atcgtgcaga ccgaccccga
     2101 cgtggccaac gtctactact ggatcggccc gaatccgacg ctgagccagg gccgcttgct
     2161 gatcaatctc aagcccttcg gcgagcgcag ctccagcgcc gagcagatcg tggcgcgcct
     2221 caagccgcgc gtggcatccg tgcagggcat cgcgctctac atgcaactgc gccaggacat
     2281 ccaggtgggc gggcgcccga gcaagacgca gttccagtac accttgcagg cggccgacat
     2341 cggcgagctg aaccactgga gcggcgtctt gcagcagcgg ctcaagacgc tgccgcagct
     2401 gaaggacgtg acctcggacc agcagcaatc cgcgcccgag gcgacg
//
LOCUS       DASUCY010000247         2304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1482823, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000247 DASUCY010000000
VERSION     DASUCY010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1482823"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="VFV25_04715"
     CDS             complement(<1..764)
                     /locus_tag="VFV25_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEY1392651.1"
                     /translation="MNMKSSAPSRSRPVVLVPACNVMRGDHPSHVVGQKYIDAVRLAG
                     CLPLVVPQAEASEVDELLDAADGVLLTGSPSNVHPSHFGEAVRDASLPLDPARDGWVL
                     PLIPRALERGIPLFAICRGAQEVNVALGGSLHQAVQEVDGKHDHRSDDDDPPEVQYGI
                     SHTVHAQRGGLLERLLGLHEFEVNSLHGQGVNRLAAGLRVEAVAPDGLVECFSKPDAP
                     GFNLCVQWHPEWQAAENPVSMKLLGAFGAACQDYRDR"
     gene            complement(832..2145)
                     /locus_tag="VFV25_04720"
     CDS             complement(832..2145)
                     /locus_tag="VFV25_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY1392652.1"
                     /translation="MYQDTKLDAYWMPFTANRQFKKAPRMLARAEGMYFYTPEDKPVL
                     DAIAGLWCVNAGHARPKIVRAIQQQAGELDFAPPFQMGHPKAFELAERLVALTPKGLN
                     RVFYTNSGSESVETALKIAIAYHRVRGEGSRTRLIGRERGYHGVNFGGISVGGMVANR
                     KMFGTLLAGVDHIRHTHDPARNAYTRGQPAHGAEFADDLERLVALHDASTIAAVIVEP
                     VAGSTGVILPPQGYLQRLREICDKHGILLIFDEVITGFGRTGAAFGAQRFGVTPDIIT
                     AAKGITNGCVPMGAVFVKREIHDAFMNGPEHLIELFHGYTYSAHPLACAAALGTLDTY
                     AEEGLLTRAAEMESCFADALHSLKGLPHVIDIRNIGLVGGIELAPRANEPAKRAFDVF
                     LDCWEHGVLIRTTGDTIALSPPLIVEKPQIERIVGSIADALKRVA"
BASE COUNT          367 a          758 c          790 g          389 t
ORIGIN      
        1 cggtcgcgat agtcctggca ggccgcgccg aaggcgccga gcagtttcat ggacaccggg
       61 ttctcggccg cctgccattc cggatgccat tgcacgcaga gattgaagcc cggcgcatcg
      121 ggcttggaga agcactcgac caggccgtcg ggggcgacgg cttcgactcg caagccggcg
      181 gcgaggcggt tcaccccctg gccatgcagg ctgttgactt cgaattcatg caggcccagc
      241 agccgctcga gcaagccgcc gcgctgcgcg tgcacggtat gcgagatgcc gtattggacc
      301 tcgggcgggt cgtcgtcgtc gctgcggtga tcgtgcttgc cgtccacctc ctgcaccgcc
      361 tggtgcagcg agccgcccag cgccacattg acttcctggg caccgcggca aatcgcgaac
      421 agcgggatgc cgcgctccag cgcacgcggg atcagcggca gcacccagcc gtcgcgcgcc
      481 gggtccagcg gcaagctggc atcgcgcacc gcctcgccga aatggctggg gtggacattc
      541 gatggcgagc cggtgagcag cactccgtcg gccgcatcga gcaactcgtc gacctcgctg
      601 gcctcggcct gcggcaccac caggggcagg cagcccgcga gacgcaccgc gtcgatgtat
      661 ttctgcccca cgacatgcga tgggtgatcg ccccgcatca cattgcaggc cggaaccagc
      721 acgacggggc ggctcctgga gggtgcgctc gacttcatgt tcatggtgct ggcagcgccc
      781 ggtccttggg cgggcgcgtc actactgagt tccagtctac gccttcggct ttcatgcgac
      841 gcgcttgagg gcatcggcga tgctgccgac gatgcgctcg atctgcggct tctcgacgat
      901 cagcggcggc gacagcgcga tcgtgtcgcc ggtggtgcgg atcagcacgc catgctccca
      961 gcagtcgagg aagacatcga aggcgcgctt ggccggctcg ttggcgcgcg gcgcgagctc
     1021 gatgcccccc accaggccga tgttgcgaat gtcgatgaca tgcggcaggc ccttcaggga
     1081 gtgcaaggca tcggcaaagc aggactccat ctccgcggcg cgcgtcagca ggccttcctc
     1141 tgcataggtg tccagcgtgc ccaatgcagc ggcgcaggcc agaggatggg ccgaataggt
     1201 gtagccgtgg aagagctcga tcagatgctc gggcccgttc atgaaggcgt cgtgaatttc
     1261 ccgcttcacg aacaccgcac ccatgggcac gcagccgttc gtgatgccct tggctgccgt
     1321 gatgatgtcg ggcgtgacgc cgaagcgctg ggcgccgaag gccgcaccgg tgcggccgaa
     1381 gccggtgatg acctcgtcga agatgagcag gatgccgtgc ttgtcgcaga tctcgcgcag
     1441 gcgctgcaga tagccttgcg gcggcaggat cacgccggtc gagccggcca ccggctcgac
     1501 gatgacggca gcaatggtcg aggcgtcatg cagggcgacc aggcgttcca ggtcgtccgc
     1561 gaactcggcg ccgtgcgccg gctgcccgcg cgtgtaggca ttgcgcgccg ggtcgtgcgt
     1621 gtggcggata tggtcgacgc cggcaagcag cgtgccgaac atcttgcggt tggccaccat
     1681 gccgcccacc gagatgccgc cgaagttcac gccgtgatag ccgcgctcgc ggccgatcag
     1741 gcgggtacgc gagccttctc cgcgcacgcg gtggtaggcg atggcgatct tgagcgccgt
     1801 ttcgaccgat tccgagcccg agttcgtgta gaagacgcga ttgagcccct tgggcgtcaa
     1861 ggccaccagc cgctcggcca gttcgaaggc cttggggtgg cccatctgga agggcggcgc
     1921 gaagtccagt tcgccggcct gctgctggat ggcgcgcacg atcttcggcc gcgcatggcc
     1981 ggcattgacg caccacaggc cggcgatggc gtccagcacg ggcttgtctt cgggcgtgta
     2041 gaaatacatg ccttcggcgc gggccagcat gcgcggcgcc ttcttgaact gccggttggc
     2101 cgtgaagggc atccaatagg catcgagctt ggtgtcctgg tacatatgag cctccatgcc
     2161 ttcgagcatg caggcagttt gccgcgattg caatcggtag gccttgcgag cgccggctcg
     2221 cgcttgatcg aggtgccagg ctgccgcaac atagccgctt gctgcgcccc aacgccatgc
     2281 ccactatcgt cttcgccccc caat
//
LOCUS       DASUCY010000248         3443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_615428, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000248 DASUCY010000000
VERSION     DASUCY010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615428"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="VFV25_04725"
     CDS             <1..557
                     /locus_tag="VFV25_04725"
                     /inference="COORDINATES: protein motif:HMM:NF025100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HEY1392653.1"
                     /translation="AAVFSSTGGDCDNCHQILETLATPERAVSPTRFHNSVHNMPAGY
                     WSIATGCMAVSTSLCAYDATFAAGLLEATTQALDSGQPCLLVAFDTAYPEPLYAQRPI
                     PYAFGAALALNPTRSAAAKATLALSLAETEVATMSEPELERLRLAVPAARSLPLMRKL
                     ANCESGAVVIDYLDDLQLVVDLQP"
     gene            560..997
                     /locus_tag="VFV25_04730"
     CDS             560..997
                     /locus_tag="VFV25_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013207021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxylacyl-ACP dehydratase"
                     /protein_id="HEY1392654.1"
                     /translation="MLDHAWIAAHIPHQGSMCLLDGVAQWSESEIVCTASSHRRLDNP
                     LRSANRLGIATGIEYAAQAMAVHGALLSAEAANSTQGYLASARDVSWQVLRLDDIEGP
                     LSVRAERLSGNASNILYQFSLQGEGRTLLTGRATVILNTATPP"
     gene            994..1737
                     /gene="fabG"
                     /locus_tag="VFV25_04735"
     CDS             994..1737
                     /gene="fabG"
                     /locus_tag="VFV25_04735"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEY1392655.1"
                     /translation="MTDSSPHSSKRALVTGGSGAIGAAICRRLARDGLHVIIHANRQT
                     VAAERLRDELITAGASAEVAQFDVTDAEATKQTLERLLEAGAIQVLVNNAGIHDDAAF
                     PGMSHEQWHRVIDVSLHGFFNVTQPLTMPMVRSRWGRIVTISSVAALTGNRGQANYAA
                     AKGALHAASKSLALELASRGITVNVVAPGIIESEMSGKTFDAEAIARLVPMRRAGRPE
                     EVADLVGFLASDQAGYVSGQVISVNGAMI"
     gene            1785..2801
                     /locus_tag="VFV25_04740"
     CDS             1785..2801
                     /locus_tag="VFV25_04740"
                     /inference="COORDINATES: protein motif:HMM:NF044415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease L-PSP"
                     /protein_id="HEY1392656.1"
                     /translation="MPLVFSNTDIHGKAESQNGHDALPATQPLGSLVHAIQPAAHAAA
                     APEFHVPMNCLTGEDKARQIWRAAGSLHTESTRSSGGVELALAESEDFLFVTAEADAD
                     LPIEQQSFTCYTALLQTLRAHRKPHPLRIWNYITRINEAEGGVERYKSFNSGRRQAFR
                     NLGFTVSEGAPAACALGKQHGPLQIAILAARRPPVPIENPRQTSAYHYPNQYGIDPPI
                     FSRAAWFQQAEGAGLLLISGTASIVGHQTLHLGDVMAQAEETVKNLQSLLDVAHRKAG
                     APLWRLADLKGRVYVRRAADYPRVKAYLESLGMHDFSYHEADVCRSELLVEIEAEGQA
                     LRAS"
     gene            2806..>3443
                     /locus_tag="VFV25_04745"
     CDS             2806..>3443
                     /locus_tag="VFV25_04745"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY1392657.1"
                     /translation="MSRLLQLLLFYLMLLWLGAMLLLGNLACLPLLAAPRSWREPLVQ
                     RLISATFRLFLHGSSACGLMRLDLTALDRLNQDREAGTGLLLVANHPSMIDVFLVISR
                     VRHAICLMKASLGLNVFLAVGAYLAGYASNSRADLMIRQAARAVSAGRLLLAFPEGTR
                     TIHQPINPLKPGVGLIAKLSKAPLQTILIRTNSAYLAKGWPIWRPPQFPLVY"
BASE COUNT          595 a         1216 c         1104 g          528 t
ORIGIN      
        1 tggccgccgt gttctcgtcc accggcggcg actgcgacaa ctgccaccag atcctcgaga
       61 ccctggccac gccggagcgt gcggtgtcgc cgacgcgctt ccacaattcg gtgcacaaca
      121 tgccggccgg ctactggagc atcgccaccg gctgcatggc ggtctcgacc agcctgtgcg
      181 cctacgacgc gaccttcgcc gccggtctgc tggaggccac gacgcaggct ctcgacagcg
      241 gccaaccctg cctgctggtg gccttcgata ccgcctatcc cgagcctctc tacgcacagc
      301 ggcccatccc ctatgctttc ggcgccgcgc tggcgctcaa ccccactcgc agcgcagcag
      361 ccaaggcgac gctagccctg agcctggccg agaccgaagt cgccacgatg agcgagccgg
      421 agctggagcg cctgcggctc gccgtacctg ccgcgcgcag cctgccgctg atgcgcaagc
      481 tcgcaaactg cgagagcgga gccgtggtga tcgactacct cgacgatctg cagctggtcg
      541 tggacttgca gccatgacga tgctcgacca cgcctggatc gccgcgcata tcccgcacca
      601 gggcagcatg tgcctgctcg acggcgtggc gcaatggagc gagagcgaga tcgtctgcac
      661 agctagcagc catcgccgcc tcgacaaccc cctgcgcagc gccaacaggc tgggtatcgc
      721 gaccggcatc gaatacgcag cacaggcgat ggcggtgcat ggcgcgctgc tcagtgcaga
      781 agcggcgaac agcacccagg gctatctggc gagcgcacgc gatgtgagct ggcaggtatt
      841 gcgcctggac gatatcgagg gaccgctgag cgtgcgcgcc gagcgccttt ccggcaacgc
      901 ctcgaacatc ctctaccagt tcagcctgca gggcgaggga cgcacgctgc tgaccggccg
      961 cgcgaccgtc atcctgaata ccgccacccc tccatgactg actccagccc gcattcgagc
     1021 aaacgcgcac tcgtcaccgg cggcagcggc gccatcggcg cggcgatctg ccgccgcctg
     1081 gcgcgggacg gcctgcacgt gatcatccat gcaaacagac agacggtcgc agccgagcgc
     1141 cttcgcgatg aactgatcac cgcaggcgcg agcgccgagg tcgcgcagtt cgacgtgacc
     1201 gatgcggaag ctaccaagca gacgctggaa cgcctgctcg aagcaggagc gatccaggtg
     1261 ctggtcaaca acgcgggcat ccacgacgac gcggcctttc cgggcatgtc gcacgagcag
     1321 tggcaccgcg tcatcgacgt ctcgctgcac ggcttcttca acgtgaccca gccgctgacc
     1381 atgcccatgg tgcgcagccg ctgggggcgc atcgtgacga tctcctcggt cgcggcgctg
     1441 accggcaacc gcggccaggc caattacgcc gccgccaagg gcgcgctgca cgcggcgagc
     1501 aaatccctag cgctggagct ggccagccgc ggcatcacgg tcaacgtggt ggctccgggc
     1561 atcatcgaat ccgagatgag cggcaagacc ttcgacgcgg aggccatcgc acgcctggtg
     1621 ccgatgcggc gcgccgggcg acccgaggag gtggccgatc tggtgggctt cctcgcctcc
     1681 gaccaggcgg gctatgtcag cggccaggtc atttcggtga acggggccat gatctgagcg
     1741 gcgatcgccg tcgcatacag agacttcaca agaagaattg atcgatgccg cttgtttttt
     1801 ccaacaccga catccacggc aaggccgaat cgcagaacgg acacgatgcg ctgcccgcaa
     1861 ctcagccgct cggcagcctg gtgcacgcga tccagcccgc ggcccatgca gcggcggcgc
     1921 ccgagttcca cgtgccgatg aactgcctga ctggtgagga caaggcccgg cagatctggc
     1981 gagccgcagg cagcctgcac accgaatcga cgcgcagcag cgggggcgta gagctggcgc
     2041 tggccgagag cgaggacttc ctcttcgtga cggccgaggc tgatgcagac ctgccgatcg
     2101 agcagcagag cttcacctgc tacacggccc tgctgcagac cctgagggcg catcgcaaac
     2161 cgcatccctt gcgcatctgg aactacatca cccggatcaa cgaggcagaa ggcggcgtcg
     2221 agcgctacaa gtctttcaat agcgggcgca ggcaggcgtt ccgcaatcta ggcttcacgg
     2281 tctcggaagg agcgccggcc gcctgcgctc tgggcaagca gcacgggccc ttgcagatcg
     2341 cgatcctagc cgcccgccgg ccgccggtgc cgatcgagaa cccgcgccag accagcgcct
     2401 atcactaccc caaccaatac ggcatcgatc cgccgatctt ctccagggcg gcctggttcc
     2461 agcaggccga gggtgccggc ctgctgctga tcagcggcac ggcgagcatc gtcgggcacc
     2521 agacgctgca cctgggcgac gtgatggcgc aggccgagga gacggtgaag aacctgcaga
     2581 gcctgctcga cgtcgcgcat cggaaggccg gcgccccgct gtggcggctg gccgatctca
     2641 aggggcgggt ctatgtccgc cgcgcggccg actatccgcg cgtcaaggcc tacctggagt
     2701 cgctgggcat gcatgacttc agctatcacg aggccgacgt ctgccgcagc gagctgctgg
     2761 tggagatcga ggccgagggc caggcacttc gtgccagctg aacgcatgtc gcgcctgctg
     2821 cagctgctgc tgttctatct catgctgctg tggctgggcg cgatgctgct gctgggcaac
     2881 ctggcctgcc tgcccttgct ggcggcgccc aggtcctggc gcgaaccctt ggtgcagcgc
     2941 ctgatcagcg ccaccttccg gctcttcctg cacggcagct cggcctgcgg gctgatgcgg
     3001 ctggacctca ccgcgctgga ccgcctgaac caggatcgcg aagcgggcac tggcctgctg
     3061 ttggtggcca accacccgag catgatcgat gtgtttctgg tgatctcgcg cgtgcgccat
     3121 gcgatctgcc tgatgaaggc cagcctcggc ctcaatgtct tcctggccgt cggcgcctac
     3181 ctggccggct atgcctccaa cagccgcgcc gacctgatga tccggcaggc ggcccgagcc
     3241 gtgtccgccg ggcggctgct gctggccttt ccggaaggca cgcgaaccat ccatcaaccc
     3301 atcaacccgc tcaagcccgg cgtcggcctg atcgccaagc tgagcaaggc gccgctgcag
     3361 accatcctca tccgcaccaa ttcggcctat ctcgccaaag gctggccgat ctggcggccg
     3421 ccgcagttcc cgctggtcta cca
//
LOCUS       DASUCY010000249         2170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_695939, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000249 DASUCY010000000
VERSION     DASUCY010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695939"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="VFV25_04750"
     CDS             complement(<1..713)
                     /locus_tag="VFV25_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012760906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEY1392658.1"
                     /translation="MQAIGHRRNLPIHDTEALLAVDLPEPTPGPHDLLVQVRAVSVNP
                     VDVKLRAGKPVPEGQTRVLGWDAAGTVLAVGAQVQGFAVGDRVWYAGAIDRPGTNAER
                     HVVDARLASRMPATLGFADAAALPLTAITAWELLFDRLAVPRGGGDGQRLLVVGAAGG
                     VGSMLLQLAHQFTALEVIATAARPESRDWAHGMGAHHVIDHRQPIDQALAAAGLQSVD
                     LVASLTQTQAHYPALVQALA"
     gene            874..1836
                     /locus_tag="VFV25_04755"
     CDS             874..1836
                     /locus_tag="VFV25_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY1392659.1"
                     /translation="MPTLRLALRVAELGSVAAAARELDLLPATATAAVRRLESQLGAA
                     LFARSTRALKVTPEGDAYLARAREALDLLDQGAAELHAPLTQVRGVIRLAVSVDLGTQ
                     VVRPLMDEFLRLHPQLQLEMLVSDRLSDIGREPVDAALRYGEPRGTDQIVRPLADNTA
                     VLVASPAYVQRAGMPRSVAELAEHEAIGLRVASRPGQPWSLQDRGREVQVRPRVRRTA
                     DNGLMARLWALDGHGIALKSRLDVAADLLSGRLVRVLPRVQTAPYPLMMVLVRGTHLS
                     ARMRALADYLRPPMQALAQSASDERLAALPPTSAARASPRRKAP"
     gene            complement(1885..2106)
                     /locus_tag="VFV25_04760"
     CDS             complement(1885..2106)
                     /locus_tag="VFV25_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3079 domain-containing protein"
                     /protein_id="HEY1392660.1"
                     /translation="MAKKFPIHPANPARICWGCDRYCPADALMCGNGTVATMHPSELF
                     GEDWMEWGLDARESENSSASVEPPGVPPA"
BASE COUNT          343 a          731 c          757 g          339 t
ORIGIN      
        1 gccagggcct ggaccaatgc cgggtagtgg gcctgggttt gcgtcaggct ggccaccagg
       61 tcgaccgatt gcagcccggc tgcagcgagt gcctggtcga tcggttggcg atgatcgatg
      121 acatggtggg cgcccatgcc atgcgcccag tcgcgggact ccgggcgcgc ggcggtggca
      181 atcacttcca gtgcagtgaa ttgatgcgcc agctgcagca gcatggagcc cacgccgccg
      241 gcggcgccga ccaccagcag gcgctgaccg tcgccgcccc cgcgcggcac ggccagccgg
      301 tcgaagagca gttcccaggc cgtgatcgcg gtcagcggta gcgcagcggc atctgcaaag
      361 cccagggtgg ccggcatgcg gctcgccagc cgcgcatcca ccacgtgccg ctcggcgttg
      421 gtgcccggcc ggtcgatggc gccggcgtac cagacgcggt cgcccaccgc aaagccctgc
      481 acttgcgcgc ccaccgcgag aacggtgccc gcagcgtccc agcccagcac cctggtctgc
      541 ccctcgggca ccggcttgcc ggcgcgcagc ttgacgtcga ccgggttgac cgagactgcc
      601 cgcacttgca ccagcaagtc gtgcggcccc ggcgtgggct cgggcagatc gaccgcgagc
      661 agggcctcgg tgtcgtggat gggcaggtta cggcgatggc cgatggcttg cataaatcac
      721 tccttcaaca aacaggttgc atgggcaggc atgttgaagg tcaatcgaaa aacaaacgag
      781 gcatggcaaa ctacatcttt agcaaattca gtttgtcaat tgatgcctcg acgagcgcaa
      841 cgccccggcc cccattcgtc ggcgccgacg gacttgccga ccttgcgcct cgccttgcgg
      901 gtcgctgagt tgggcagcgt ggccgcggcc gcccgcgagc tggacctgtt gccggctacg
      961 gccacggccg ccgtgcgtcg gcttgaatct caactgggcg cggccttgtt cgcgcgcagc
     1021 acgcgagccc tgaaggtcac gccggaaggc gacgcctacc tggcgcgggc gcgcgaggcg
     1081 ctggatttgc tggatcaggg tgcagccgag ctgcacgcac ccttgacgca ggtgcgcggg
     1141 gtcatccggc tggcggtctc ggtggacctg ggcacgcagg tggttcgtcc gctgatggac
     1201 gaattcctgc ggctgcatcc gcagctgcag ctcgagatgc tggtgagcga ccggctcagc
     1261 gacatcggcc gtgagccggt cgacgcggcg ctgcggtatg gcgaaccgcg cggcaccgat
     1321 cagatcgtgc gccctctggc cgacaacact gcggtgcttg tggcctcacc cgcctatgtg
     1381 cagcgggccg gcatgccgcg cagcgtggcc gagctggccg agcatgaggc catcggcctg
     1441 cgcgtggcca gcaggccggg tcagccgtgg tcgctgcagg acaggggccg cgaggtgcaa
     1501 gtcaggcccc gcgtgcgccg caccgccgac aacggattga tggcgcggct atgggcgctg
     1561 gacgggcatg gcatcgcctt gaaatcgcgc cttgacgtgg ccgccgacct gctgtccggc
     1621 cggctggtga gggtgctgcc gcgggtgcag accgcgcctt atccgctgat gatggtgctg
     1681 gtacgcggca cgcacttgag cgcgcgcatg cgcgccctgg ccgactatct gaggccaccc
     1741 atgcaggctc ttgcgcagag cgccagtgac gagcgcctcg ctgcgctgcc gccaacaagc
     1801 gccgcgcgag catcccctcg gagaaaggcg ccttgatcag gcttgcgtca gcgcggcctg
     1861 ttcccgcgct gatgcacgtg caacctaggc aggcggcact cccggcggct caacggaagc
     1921 cgaagagttc tcggactccc tcgcatccag cccccactcc atccaatcct cgccgaacag
     1981 ctcggacgga tgcatggttg caaccgtgcc gttgccgcac atcaacgcat cagcggggca
     2041 atacctatcg cagccccagc agatgcgcgc ggggttggcg gggtggatag ggaacttctt
     2101 ggccatgcga acggctcggg agcggttttg gatggagcgc tgcgcatgaa gaatgccgct
     2161 tagccaagcg
//
LOCUS       DASUCY010000250         5366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_519352, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000250 DASUCY010000000
VERSION     DASUCY010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 5366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5366
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519352"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="VFV25_04765"
     CDS             complement(<1..719)
                     /locus_tag="VFV25_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY1392661.1"
                     /translation="MKSIAVVGGGITGVTTAYVLARHGFAVTLYEKHRYVAMETSFAN
                     GGQLSASNSEVWTHPSTILKGLKWMLKSDAPLLVNPRPTWHKLSWFAEFIASMAEYRQ
                     NTVQTACMAIAAREHLFGWADEEGIEFDLRREGILHIYRDKAGFDHAGRVSKLLAEGG
                     LSRRAVTAAEIRAIEPALSGAYYGGYYTESDATGDIHKFTYGLAQAAAQRGVHLRHGA
                     EVSRLTAEGRGVRLSVGRGPQ"
     regulatory      complement(890..977)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(1301..2596)
                     /locus_tag="VFV25_04770"
     CDS             complement(1301..2596)
                     /locus_tag="VFV25_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1392662.1"
                     /translation="MSDPRAPSRLGAVIRVTSGNFIEMYDFFLFGFYAGAISHAFFPA
                     SSEYASLMLTFATFGAGFLMRPVGAIVLGAYVDRVGRRKGLLLTLAIMALGTALVAFV
                     PRYDSIGLMAPILVLAGRLLQGLSAGVELGGVSVYLSEIAPPGRRGFFVSWQSGSQQL
                     AIVVSALLGYGLNAWLSHELVDAWGWRLPFFIGCLIVPAIFAIRRSLQETMVFQASGH
                     RPSFTQTLQGIRTHWRTITLGTLLIIMTTVSFYLITVYTPTFGKSVLKLSTAESLLVT
                     LAVGVSNLAWLPLMGALSDRIGRAPIMMAATALTLVSAYPAMNWLVGQPSLGRMLAVE
                     LWLSFLYASYNGATIVALTEVVPGHIRTAGFSLAYSLATALFGGFTPLLCTWLIGATG
                     NRAMPGLWMAAAALVSLLATLAIYGRRGDRRSGPAMATS"
     gene            complement(2662..3438)
                     /locus_tag="VFV25_04775"
     CDS             complement(2662..3438)
                     /locus_tag="VFV25_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015601153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY1392663.1"
                     /translation="MLRPLLPVLLSASLCLFATVTPAAEIGVASSGGFAAAFKTLASR
                     YEAGAPNRGVRRIFGPSMGNTPEAVPARLARGEEIDVVIMVGEALDQLMARGLLEPGS
                     KVLLAQSRIACAVPAGASRPRLGSEDEVRQAFLQAPSVAWSDSASGEYIQKTLLARLG
                     IESEVRAKGRQIPGTPVGEIVARREAMFGCQQRSELRPVAGIDIVGDLPDALQKVTPF
                     AAAVVRGSRHPQDARAFIDFLASPGSAPLIEATGLAPAPH"
     gene            3558..4454
                     /locus_tag="VFV25_04780"
     CDS             3558..4454
                     /locus_tag="VFV25_04780"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY1392664.1"
                     /translation="MICNGLDLEQLKAFVAVADLRSFRRAAEELNLSAPALSRRIERL
                     EDRVGARLLERTSRSVRLSRVGESFVERVRAVLQDLDAAVLDVGELSSGHRGRVTLAA
                     IPSAAIGLVPRALADFTRQLPEVQVRVLDGSLQATQLAVLGGQADLGVGFQDTPVAGL
                     HAETLGVDPYVLAVPAGHRWAARRSLRLPEIARERLLSLASGSGNRQLLDSQLLQLGL
                     TLPPAHEAAHIGALLGLVEAGLGCALVPSMALRLPPPGLRAVRLQSNPLGREIVIFSS
                     QERRLTPLAQRLLPALRRAFTG"
     gene            4732..>5366
                     /locus_tag="VFV25_04785"
     CDS             4732..>5366
                     /locus_tag="VFV25_04785"
                     /inference="COORDINATES: protein motif:HMM:NF012576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY1392665.1"
                     /translation="MPTSKSKPPTATPPKTRRRSDAPTLLDVARTAGVSEMAASSALN
                     GGRRGSARVSAETRERVLAAAQRLGYRPNATARALANRRTNAIGFVTDSPGEELNLYF
                     LEVFNGVIQGATAAGQSTAVFTLSDWNEAPARIPALCDGRVDGLILLGPRLQDDGSAW
                     LPQHTPLVSVQADRPMSGVVNIESDDEAGAHEMVRQLLAIGHRRILHVGGPP"
BASE COUNT          889 a         1797 c         1840 g          840 t
ORIGIN      
        1 atctgcgggc cacggccgac gctgaggcgc accccgcgcc cctcagcggt gagcctgctt
       61 acttcggcgc cgtggcgcag gtgcacgcct cgctgcgcgg ccgcttgtgc taggccgtag
      121 gtgaacttat ggatatcgcc cgtggcatcg ctttccgtgt agtagccgcc gtagtacgca
      181 ccggacagcg cgggctcgat tgcacggatt tccgcagccg tcacggcacg ccgtgacaga
      241 cccccttccg cgagcagctt cgacacccgc ccggcgtggt cgaagccggc cttgtcacga
      301 tagatgtgga ggatcccttc gcgccgcaga tcgaactcga tgccctcctc gtcagcccag
      361 ccgaacaggt gctcgcgcgc ggcgatcgcc atgcacgccg tctgcaccgt gttctggcgg
      421 tactccgcca tgctcgcgat gaactcggcg aaccaggaca gcttgtgcca agtgggcctt
      481 ggattgacca acaatggcgc atcgctcttg agcatccact tcaggccctt gaggatggtg
      541 gacgggtggg tccacacctc agagttggac gcggacaact gcccgccgtt ggcgaacgag
      601 gtctccatgg ccacgtagcg gtgcttctcg tacagcgtca ccgcgaaccc atggcgcgcc
      661 agcacatagg ccgtcgtgac gcccgtgatg ccgccaccga caactgcgat cgatttcatt
      721 gctctcagct tccatccgtc gaggggttgg aggtcccagc gcgacatgcg ctgcgaacgc
      781 cccctctgtt tcggacctga gagattcacg ccctgcgctg ggatgcggac ggcgcttgct
      841 ccttcggtgc tgccggatcg acgacgtcgg gcagctcttc agagtcagtg aagcgacgtc
      901 ggtcctgtgg cctgagagtt tccggggcgg ttgctccttc ggcgttgccg gttggcaagc
      961 tctcccgaca tcgcatcgac ccgtgagtcg acttgtgagg agcattgtgc ccgagtcgcc
     1021 ttcaaaggga gcaacatgtt gccgccgacc gcggattggg ccagatatcg gacatgtgcc
     1081 gatattccgg tgaaccaccc gccaccccaa gccagcgagg catgatgcgg gtccgagacg
     1141 gatggcctgg ctcaatgaca tatcggcgcg ccggctcatt ccggagtcgg cgcctacatg
     1201 ccgtcgcccg atggcacgga attggaccgg atcgacggcc cttctggggt cttcgcagtg
     1261 gcgcagtaga ccagcgctcg cgagggatgg gcctgcagga tcaggaggtc gccatcgccg
     1321 gaccggagcg gcggtcaccg cgccggccat agatcgccag cgtcgccagc aggctaacca
     1381 acgccgccgc ggccatccaa agcccgggca tcgcgcgatt gccagtggcc ccgatcagcc
     1441 aggtgcagag cagcggcgtg aagccgccga acagcgcggt ggccaggctg taggccagcg
     1501 agaaacccgc ggtgcggatg tggccgggca cgacttcagt cagcgcgacg atggtcgccc
     1561 cgttgtagct ggcgtacagg aaggacagcc acagctcgac cgccagcatg cggcccaggc
     1621 tgggctgccc gaccagccag ttcatcgccg gataggccga caccagcgtc agcgcggttg
     1681 ccgccatcat gatcggtgcg cggccgatgc ggtcggacag cgcccccatc agcggcagcc
     1741 aggccaggtt ggagaccccc acggccagcg tgaccagcag gctctcggcc gtcgacagct
     1801 tcaacaccga cttgccgaag gtcggcgtgt agacggtgat cagataaaac gacactgtgg
     1861 tcatgatgat cagcagcgtg cccagcgtga tcgtgcgcca gtgagtgcgg atgccttgca
     1921 gtgtctgcgt gaaactcgga cggtggccgg aggcctggaa caccatggtt tcctgcaggc
     1981 tgcggcggat cgcgaagatc gccggcacga tcaggcagcc gatgaagaat gggaggcgcc
     2041 agccccaggc gtccaccagt tcgtgcgata gccaagcgtt cagcccatag cccagcaagg
     2101 cggagaccac gatggccagc tgctgactgc cagactgcca gctgacaaag aagccgcggc
     2161 gtccgggcgg cgcgatctcc gacaggtaga ccgaaactcc gcccagctcg acgccggccg
     2221 acaggccctg caggagtcgg cccgccagca ccaggatcgg cgccatcagc ccgatgctgt
     2281 catagcgcgg aacgaaggcc accagtgccg tacccagcgc catgatcgcc agcgtcagca
     2341 gcaggccctt gcggcggccg acgcggtcga cgtaggcccc cagcacgatc gcaccgaccg
     2401 ggcgcatcag gaagcccgcg ccaaaggtcg cgaaggtcag catcagcgac gcgtactcgc
     2461 tgctggccgg gaagaatgcg tgtgagatgg cgccggcata aaagccgaac agaaagaagt
     2521 cgtacatctc gatgaagttg ccgctggtca cgcggatcac tgcaccgagc cgggatgggg
     2581 cgcgggggtc ggacatgctg tctcctgggc ctgggaacgg gcctgtcgtt gtgggaggaa
     2641 aacgggaagg gcccgcgacg ctcagtgcgg cgcgggcgcc agtccggtgg cctcgatcag
     2701 cggcgcgctg ccgggcgaag ccaggaagtc aatgaaagcg cgcgcgtcct gaggatggcg
     2761 gctgccgcgt accaccgcgg cggcgaacgg cgtgaccttc tgcagagcat cgggcaggtc
     2821 gccaacgatg tcgatgccgg ccacgggccg cagctcgctg cgctgctggc aaccgaacat
     2881 cgcctcgcgc cgcgcgacga tctcgcccac gggcgtgccc gggatctgcc ggcccttggc
     2941 gcgcacctcc gactcgatgc ccagccgggc tagcagggtc ttctggatgt attcgccgct
     3001 ggcgctgtcg gaccaggcca ccgaaggggc ctgcaagaag gcctggcgga cttcgtcctc
     3061 gctgcccagc cggggccgcg aggcgccggc cggtaccgcg caggcgatgc gcgactgcgc
     3121 cagcagcacc ttgctgccgg gctccagcag gccgcgggcc atcagctggt ccagcgcctc
     3181 gccaaccatg atgaccacgt cgatctcctc gccgcgggcc aggcgcgcgg gcaccgcctc
     3241 gggcgtgttg cccattgacg ggccgaagat ccgacgcacg ccgcggttcg gcgcgccggc
     3301 ctcgtagcga ctggccagcg tcttgaaggc cgcagcgaag ccgcctgagg acgccacgcc
     3361 gatctcggcg gcgggggtga ctgtggcaaa caagcacagg ctggccgaca gcagcaccgg
     3421 cagcagcggg cggagcatga ggggcagtcg attcggcttc atggcgaacg gcgcggcggc
     3481 gcgcaggttg gcggggtgcg cacatgatgg gtgagcatcc cgtatgcgtc cattgcaacc
     3541 tttcttatga tggatgcatg atttgcaatg gtctggatct ggaacagctc aaggccttcg
     3601 tggccgtggc cgacctgcgc agcttccgcc gcgcggccga ggagctcaac ctctcggcgc
     3661 cggccctgag ccgccgcatc gagcgcctgg aggaccgggt cggcgcgcga ctactggagc
     3721 gcacctcgcg ctctgtgcgg ctgtcgcgcg tcggcgaatc cttcgtcgag cgggtgcgcg
     3781 ccgtgctgca ggacctggat gccgcggtgc tggacgtggg cgagttatct tccggccacc
     3841 gcggccgggt cacgctggca gccatcccat ccgctgctat tgggctggtg ccgcgggcgc
     3901 tggcggactt cacacgccag ctgcccgagg tgcaagtgag ggtgttggac ggatcgctgc
     3961 aggccacgca gctggctgtg ctgggcgggc aggccgacct gggcgttggc ttccaggaca
     4021 caccggtggc tgggctgcac gccgagaccc tgggggtcga tccctatgtg ctggccgtgc
     4081 cggctggtca ccgctgggcc gcacgccgca gcctgcgcct accggagatc gcacgcgaac
     4141 ggctgctatc gctggctagc ggcagcggca atcggcaact gctggacagc caactcctgc
     4201 agctgggcct gacgctgccg ccggcgcacg aagccgcgca catcggcgcg ctgctgggcc
     4261 tggtcgaggc cggcctgggt tgcgcgctgg tgccgtcgat ggctctgcgc ttgccgccgc
     4321 cggggctgcg ggcggtgcgc ctgcagagca accccctggg ccgggagatc gtgattttca
     4381 gctctcagga gcgtcgacta accccactgg cgcagcgcct gctgccggcg ctgcgccgcg
     4441 cctttaccgg ctaaaggccg atcagaactg gtgcgcctcc atgaacatcg aacagtgccc
     4501 tgtgacagcc acggcaagcc ttcgttgtat tgatgcggaa tttgcaccat tggaatgacg
     4561 ccctatgagt tgtctcgcat gccaggtgaa ccgaggcacc gaggaaggtc tgccatggag
     4621 ccgtgtggtc gactaccctt cttgaccggg actaccactt acgaaggccg gtgctacagt
     4681 gcgtcgccgc acgtcgtgcc gcgagcggcg ttgcgcctgc gctgaacatc cgtgcccact
     4741 tcgaagtcga aaccgcctac ggcgacaccg ccgaagaccc gccgccgcag cgacgcacca
     4801 acgcttctgg acgtcgcccg cacagcaggc gtgtcggaaa tggcggcctc ctccgcgctc
     4861 aacggtggtc gtcgaggctc ggcgcgcgtg tcggccgaga cgcgcgagcg cgtgctggcc
     4921 gcggcgcagc ggctagggta caggccgaac gccaccgctc gcgcgctcgc gaaccggcgc
     4981 acgaatgcga tcggcttcgt caccgactcg ccgggcgaag aactgaacct ctacttcctt
     5041 gaagtgttca atggcgtcat tcagggcgcc accgccgccg gacagtcgac ggccgtcttc
     5101 acgctgagcg actggaacga ggcaccagcc cggatcccgg ccttgtgcga cggccgcgtc
     5161 gatggtctga tcctgctcgg cccgcgtttg caggacgacg gctcggcgtg gttgccgcag
     5221 cacacgccgc tggtgtcggt gcaagccgat cgtccaatga gcggggtagt gaacatcgag
     5281 tcggacgacg aggctggtgc tcatgagatg gtgcgccagt tgctcgcgat cgggcaccgc
     5341 cgcatcctgc acgtgggcgg cccgcc
//
LOCUS       DASUCY010000251         2404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_455138, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000251 DASUCY010000000
VERSION     DASUCY010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455138"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VFV25_04790"
     CDS             <1..595
                     /locus_tag="VFV25_04790"
                     /inference="COORDINATES: protein motif:HMM:NF017722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arylsulfotransferase family protein"
                     /protein_id="HEY1392666.1"
                     /translation="AFTWLSTDHPELYACSTDRNNFAANNGADYARWDSVAVDGDGNW
                     LASFRHLDAVLKIARSSGSVSWILGGPCDQFGLSAAQKFSHQYDARRAPDGRLTLFDD
                     NVAGGASRVLAFGLNEAAKTVTSFNAYTSDPHASISLGSAQLFADGKVLVGWGVFPGA
                     ASDVTEFDTASGAASFQLTLLGNAFAGGYFSYRARKY"
     gene            726..1613
                     /locus_tag="VFV25_04795"
     CDS             726..1613
                     /locus_tag="VFV25_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEY1392667.1"
                     /translation="MDGPSEAAATPSSRQAAAGQRVVIYRHRLPVRVMHWINVLCMAI
                     LLMSGLGIFNAHPALYWGKDSGFASPWLAIKAEQKPGGALAGKTQIGDRQWETTGVLG
                     LSKVDGEATSRAFPSWATVPGPQWLSMSRRWHFFFAWLFVINGIAYVLYTIFSRHLTR
                     DLVPTLAELRAIGASIKDHLLLKHPSGEAARRYNVLQNLSYLIVVFGLLPLVVLAGLA
                     MSPRIDTVFTGWVDWLGGRQSARSLHFLAAGGLLLFTLIHVFEVIINGVWNEIRSMLT
                     GWYRLPPEPKTDRRQQVSR"
     gene            1610..>2404
                     /locus_tag="VFV25_04800"
     CDS             1610..>2404
                     /locus_tag="VFV25_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEY1392668.1"
                     /translation="MKTFTLMRARQRERRQLLHGAVAAAGAGLLAGCDRLSNNDSFVA
                     TLESAERLSGAAQKLVAPRPAMAQEFELRDVPQTFRSNGTLDPRDEDYQALRRGGFAD
                     YRLSIAGLVAKPAEYTLAQLRALPARTQITRHDCVEGWSCIGKWKGVPLKLLLDAAQP
                     LPRARFVVFRCFDSMDEESGDEDSHYYESVDFDDAFHPQTILAYELNDQPLPVSNGAP
                     LRCRIERQLGYKHAKYVRTIELVESFAQIRGGRGGYWEDQGYEWYAG"
BASE COUNT          371 a          798 c          797 g          438 t
ORIGIN      
        1 ggcgttcacc tggctctcca ccgatcaccc cgagctttat gcctgctcga cggaccgcaa
       61 caacttcgca gcgaacaacg gcgccgacta tgcgcgctgg gactccgtgg cggtcgacgg
      121 cgatggaaac tggctggctt cgttccggca tctggacgcg gtactgaaga tcgctcgttc
      181 cagcgggagc gtttcctgga tcctcggcgg gccctgcgac cagttcggct tgagcgccgc
      241 ccagaagttc tcgcatcaat acgacgcccg gcgggcgccg gatgggcgct tgacgctgtt
      301 cgacgacaac gttgccggcg gcgcgtcgcg ggtgctggcc ttcggattga acgaagcggc
      361 caagaccgtg acgagcttca atgcctacac atccgatccg cacgccagca tctcgctcgg
      421 cagcgcccag ctgtttgccg acggcaaggt gctggtcggc tggggcgtct ttcccggcgc
      481 cgccagcgac gtgacggagt tcgacaccgc cagcggcgcg gcgtcgttcc agctcacctt
      541 gctgggcaac gccttcgcgg gcgggtattt ctcgtacagg gcccgcaagt actaggggct
      601 gttgcgggcg ctccctcacc gacctgcgtt tgctcctcta cgcctgccgg gcatctttgg
      661 cgcatcattg gttcctggaa ggcctgcccg ccgggcggtc gccgaccatg tgcggaggac
      721 aggccatgga tggtccaagc gaagcagcgg ctacgccgtc gtcgcggcaa gctgccgctg
      781 ggcagcgcgt cgtcatctac cgccaccgcc tgccggtgcg cgtgatgcac tggatcaacg
      841 tgctgtgcat ggccattctg ctgatgagcg ggctcggcat cttcaatgcc catccggcgc
      901 tctactgggg caaggattcc ggcttcgcct cgccctggct cgcgatcaag gcagagcaga
      961 agccgggcgg cgcgctcgcc ggcaagacgc agatcggcga tcgccagtgg gagaccaccg
     1021 gcgtgctggg cctgtccaag gtcgacggag aagctacgtc gcgcgccttc cccagctggg
     1081 cgacggtgcc cggaccgcaa tggctgtcga tgagccgtcg ctggcatttc ttctttgcct
     1141 ggcttttcgt catcaatggc attgcctatg tgctctacac gatcttcagc cggcacctga
     1201 cgcgtgacct cgtgccgaca ctggccgagc tgcgcgccat cggggcctcg atcaaggacc
     1261 atctgctgct caagcatccg agcggcgagg cggccaggcg ctacaacgtg ctgcagaacc
     1321 tgagctatct gatcgtagtc ttcggcctct tgccgctggt cgtgctcgcc ggcctggcca
     1381 tgtcgccgcg catcgacacg gtgttcaccg gctgggtcga ttggctgggc ggccggcaat
     1441 cggcgcgctc gctgcatttc ctggcggccg gcggtctgct gctgttcacg ctgatccatg
     1501 tcttcgaggt gatcatcaat ggggtctgga acgagatccg ctcgatgctc accggctggt
     1561 acaggctgcc gcccgaaccc aagactgacc gcagacaaca ggtgtcgcga tgaagacctt
     1621 caccctgatg cgtgcacgcc agcgcgagcg gcgtcagctg ctgcatggtg cggtggccgc
     1681 tgccggcgcc ggcctgctgg ccggttgcga ccgcttgtcg aacaacgact ccttcgtcgc
     1741 cacgctggaa agtgccgagc ggctcagcgg tgcggcccag aagctggttg cgccgcggcc
     1801 ggcgatggcg caggagttcg agctgcgcga tgtccctcag accttccgca gcaacggcac
     1861 gctggacccg cgtgacgagg actaccaggc cttgcgccgc gggggcttcg cggactaccg
     1921 cctctcgatc gccggcctgg ttgcgaagcc tgccgaatac acgctggcgc agttgcgtgc
     1981 cttgcccgca cgcacccaga tcacgcgcca tgactgcgtc gagggctgga gctgcatcgg
     2041 caaatggaag ggcgtgccgc tgaagctgct gctggatgcc gcacagccgc tgccgcgggc
     2101 gcgcttcgtc gtcttccgct gcttcgattc gatggacgag gagtccggcg acgaggactc
     2161 tcactactac gagagcgtcg acttcgatga tgccttccac ccccagacca tcctggccta
     2221 cgagctgaac gaccagccct tgccggtcag caacggcgcg ccgctgcgct gccgcatcga
     2281 gcgccaactc ggctacaagc acgccaagta tgtgcggact atcgagctgg tcgagagctt
     2341 cgcgcagatc cgtggcggca ggggggggta ttgggaggat cagggctacg agtggtatgc
     2401 gggg
//
LOCUS       DASUCY010000252         2762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1531396, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000252 DASUCY010000000
VERSION     DASUCY010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1531396"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            234..647
                     /locus_tag="VFV25_04805"
     CDS             234..647
                     /locus_tag="VFV25_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HEY1392669.1"
                     /translation="MKTTRRLLTGFVAVCISYAGALQSAQAALVGTEDVAAAQGVVSA
                     DMQAEQARSHVNAILDRSDVISGLAARGVDVADARARVAALSDMEVMHLAKTLDEAPA
                     GADDVLGTILFIFILLLITDILGFTKIFPFTRSIR"
     gene            647..1606
                     /locus_tag="VFV25_04810"
     CDS             647..1606
                     /locus_tag="VFV25_04810"
                     /inference="COORDINATES: protein motif:HMM:NF033920.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA2778 family cysteine peptidase"
                     /protein_id="HEY1392670.1"
                     /translation="MNLRRLGRLLCALAAAALLSACAPFVPPQTAALQAQRPTDLPER
                     IELGATPFHPQTEFQCGPAALATLLGAAGVEADPTQLADEVFVPARKGSLQVEMLASA
                     RRHGVLAVRLPGELEAVLRELAAGHPVLVMQNLGLSFVPAWHYAVAVGYDLERSDLIL
                     RSGRTARELLAFRTFEHTWARSGHWAFAAMPPGQLPATASEVEVTAASVAFERVAPPA
                     QAVQAYAAALQRWPASLTLAMGLGNARAAQGDVEAAAQTFAQAAQSHDSAAAWNNLAQ
                     MRLKQGRKAEAVEAARKAVQRAQAAEPQWLAAARSTLDEAEAH"
     gene            1745..2392
                     /locus_tag="VFV25_04815"
     CDS             1745..2392
                     /locus_tag="VFV25_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY1392671.1"
                     /translation="MSKLKVQSFSVSIDGYGAGPDQSLDNPLGVGGMSLHQWAFATRT
                     FQRMHGNGSDGGATGADDDFAARGFANIGAWILGRNMFGPVRGPWPDDSWKGWWGANP
                     PYHVPVFVLSHYPRESITMEGGTVFHFVTDGIEAALRRAREAAQGRDVRLGGGVATIR
                     QYLQAGLVDEMHLAISPTLLGRGEALLAGIDLTALGFRCTEHVSTPAAMHVVLTR"
     gene            2457..>2762
                     /locus_tag="VFV25_04820"
     CDS             2457..>2762
                     /locus_tag="VFV25_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY1392672.1"
                     /translation="MTQAIAYLSFNGNCAEAMRFYEKVLKAKLEVLMSGAQSPMADQM
                     PKEFADRILHARLALPGGGLLYAGDAPPNVPYEGIKGVTIALDYATVAEAEEIFNALA
                     "
BASE COUNT          433 a          943 c          929 g          457 t
ORIGIN      
        1 ctcgccgtgg gccccgcgag accgagccag cgcaggcgtc gtggtccggc ctgcgaacgg
       61 ccgctcacca tgcggggcgc cacgaagtaa caaacatggg gcgagcgtat caggccgggt
      121 tgcgggttcg cgcgcgttgc atttcgtgac tccgccgccg ctgccgacgt tacatttata
      181 ggagctgcgc atctctctgt gtcgcagcca tacccgcaaa aggagttcga aaaatgaaga
      241 cgacccgtcg cttgctgacc ggtttcgtgg cggtatgcat cagctacgca ggcgccttgc
      301 aaagcgccca agccgccctg gtaggtaccg aagatgtcgc cgccgcgcaa ggcgtggtct
      361 cggccgacat gcaggccgag caggcccgca gccatgtcaa tgccatcctc gatcgtagcg
      421 atgtgatttc gggcctggcc gcgcgcggcg tggacgtggc cgacgcgcgc gcccgcgtcg
      481 ctgcgctgag tgacatggaa gtgatgcatc tggccaagac cctggacgag gctcctgccg
      541 gcgccgacga cgtgctgggc accatcctct tcatcttcat cttgctgctg atcaccgaca
      601 tcctcggctt caccaagatc ttcccgttca cgcgctcgat tcgttgatga acttgcgccg
      661 gctcggcagg ctgctgtgcg cactcgcggc agcagccctg ctgagcgcct gcgccccttt
      721 cgtccctccg cagaccgcgg cgctgcaggc gcagcgcccg accgatctac cggagcgcat
      781 cgaactcggc gcaaccccct tccatcccca gaccgaattc cagtgcgggc cggccgcgct
      841 agcgacgctg ctgggtgcgg ccggtgtcga ggccgatccg acgcagctgg ccgacgaagt
      901 cttcgtgccg gcgcgaaagg gcagcctgca ggtcgagatg ctggccagtg cgcggcgtca
      961 tggcgtgctg gccgtacgcc tgccgggcga gctggaggcc gtgctgcgcg agctggcggc
     1021 cggccacccg gtgctcgtga tgcagaacct gggcctgagc ttcgtgccgg cctggcatta
     1081 cgccgtcgcg gtgggttacg acctggagcg cagcgatctg atcctgcgct cgggccgcac
     1141 cgcccgcgag ctgctcgcct tccgcacctt cgagcacacc tgggcgcgct cgggccattg
     1201 ggccttcgcg gccatgccgc cagggcagct gccggccacg gccagcgaag tcgaagtcac
     1261 ggcggccagc gtggccttcg agcgcgttgc gccgccagcc caggcagtgc aggcctatgc
     1321 ggcggcgctg cagcgctggc cggccagcct cacgctggcg atgggcctgg gcaacgcgcg
     1381 ggcggcacag ggcgatgtgg aagccgccgc gcagaccttc gcgcaggctg cgcagagtca
     1441 cgacagcgcg gcggcctgga acaacctggc ccagatgcgc ctcaagcagg gccgcaaggc
     1501 cgaggccgtg gaggcggcgc gcaaggcggt gcagcgcgcc caggccgccg agccgcaatg
     1561 gctcgccgcc gcgcgctcga cgctggacga ggccgaggcg cactgatttt tgcgcggtct
     1621 gtcgattcgg cgtcgcctcg ttcgtcgtat cgttgcagcc cccgatcttc gatcgactcc
     1681 cggctgctcc ttcttgtttg cgccgcgagg cgatcgatcc tcagaccatg ggagaaaagc
     1741 acgtatgtcc aagctcaagg ttcagagctt ttcggtgtcc atcgacggct acggggccgg
     1801 ccccgatcag agtctcgaca acccgctggg cgtcggcggc atgagcctgc atcaatgggc
     1861 ctttgccacc cgcacctttc aacgcatgca tggcaatggc agtgatggcg gcgcgaccgg
     1921 cgctgatgac gatttcgcgg cgcgcggctt cgccaacatc ggcgcctgga tactggggcg
     1981 caatatgttc ggcccggtgc gcggaccctg gcccgacgac agctggaagg gctggtgggg
     2041 cgccaacccg ccttaccacg tgccggtctt cgtgctgagc cattacccgc gcgagtcgat
     2101 cacgatggaa ggcggcacgg tcttccattt cgtcaccgac ggcatcgagg ccgcgctgcg
     2161 tcgcgcccgc gaagcggccc agggccgcga cgttaggctg ggcggcggcg tggcgaccat
     2221 ccgccaatac ctgcaggccg ggctggtgga cgagatgcac ctggccattt ctcccaccct
     2281 gctcggccgc ggcgaggccc tgctcgcagg tatcgacctg accgcgctgg gctttcgatg
     2341 caccgagcac gtcagcacgc cggcagcgat gcatgtcgtg ctgacgaggt agcgccgctt
     2401 cggcctcgcc gtgatgcggc ttctatccac tttcgcctcc caagaaagga agcaagatga
     2461 cgcaagctat cgcctatctg tccttcaacg gcaactgcgc cgaagccatg cgcttctacg
     2521 agaaggttct caaggccaag ctcgaggtgt tgatgagcgg cgcgcaatcg ccgatggcgg
     2581 accagatgcc caaggagttt gccgaccgca ttctgcacgc gcggctcgcg ctgcctggcg
     2641 gcgggctgct ctatgcgggc gatgccccgc cgaacgtacc ctatgaaggg atcaagggcg
     2701 tcaccatcgc cctggattac gcgacggtgg ccgaagcgga ggagatattc aacgcgctgg
     2761 cc
//
LOCUS       DASUCY010000253         4944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_455166, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000253 DASUCY010000000
VERSION     DASUCY010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4944
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455166"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            361..2043
                     /gene="nadB"
                     /locus_tag="VFV25_04825"
     CDS             361..2043
                     /gene="nadB"
                     /locus_tag="VFV25_04825"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEY1392673.1"
                     /translation="MPAPRPPTSQQAPVVIIGAGLAGLTVALHLAEQHPVVVLAKRNL
                     DEGATAWAQGGIVGVLGDDDSIESHVRDTQDAGAGLVDEETARFIAEHSAAAIEWLVA
                     RGVPFSPDPSGPLGLHLTREGGHAVRRIAHAADATGKAIHDVLLREVQRHPNITLRER
                     WMAVDLITSRHLKREEPSRCYGVYALDIDSQRVETLPAAAVVLATGGAGKVYRYTTNP
                     DTSTGDGIAMAWRAGCRVGNMEFIQFHPTCLYHPQERSFLITEALRGEGGQLKLPSLG
                     PGDPGGSRFMPAHDERAELAPRDIVARAIDFEMKKHGLDYVLLDATHLGETFLKEHFP
                     TIYARCLALGLDIAKQPIPVVPAAHYTCGGVVTDLDGRTDLPGLYAVGETTYTGLHGA
                     NRLASNSLLECVVLGRTCAQYVLAHRASVEVDLPAWDESQVEDADEQVVISHNWDELR
                     LLMWNYVGIVRTTKRLERALHRIKLLRKEIDEYYAAFRVSRDLLELRNLVECAELIVR
                     SALMRQESRGLHYSRDFPYALPVSFPTVLVRAARSGANGKGETKHKAVGLSG"
     gene            complement(2068..2352)
                     /locus_tag="VFV25_04830"
     CDS             complement(2068..2352)
                     /locus_tag="VFV25_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late competence development ComFB family
                     protein"
                     /protein_id="HEY1392674.1"
                     /translation="MVQSFESIQNFYEQPVFDAILAAAPNYPHLGAELLADAACVALN
                     RLPTRYIRHSVDLAFYMPDKERVDNERTIAEVVQHALEFVQARVAMRARR"
     gene            complement(2458..3321)
                     /gene="nadC"
                     /locus_tag="VFV25_04835"
     CDS             complement(2458..3321)
                     /gene="nadC"
                     /locus_tag="VFV25_04835"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEY1392675.1"
                     /translation="MLNNETLEEARARNVREALFEDLGRCDWTGLLLPEAERVRARVL
                     VREEAVLCGRDWFEACVHALDAEARIDWHYAEGAHMTADSLVCHIEGQARALLAAERP
                     ALNFLQLLSGVASTTRRHVDAVAGASTNPRGCAILDTRKTLPGLRLAQKYAVRVGGGQ
                     NQRLALYDGILIKENHIAAAGGVTQALKAAQALDAEVGIQIEVETLEQLREALAAGAT
                     SVLLDNFSYAQMREAVKVNAGRALLEVSGGIALEQLREIAATGVDRISIGRLTKDVKA
                     VDYSMRVLERL"
     gene            complement(3403..4515)
                     /gene="nadA"
                     /locus_tag="VFV25_04840"
     CDS             complement(3403..4515)
                     /gene="nadA"
                     /locus_tag="VFV25_04840"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEY1392676.1"
                     /translation="MSQVLFDYTKQDAQGVSCTAHAWAKVPEPLGPQARREWKARAGA
                     LLKRHKAVLVAHYYVDGDIQDLALETGGCVSDSLEMARFGRDHESQTLVVAGVRFMGE
                     TAKILSPHKRVLMPDLEANCSLDLGCPPDEFAAFCDAHPDRKVVVYANTSAAVKARAD
                     WMVTSSCALAIVQQLSAQGQKVLWAPDRHLGRYIEENTGADMLMWNGECIVHDEFKGI
                     ELELLRREHPAAKVLVHPESPKSVVEQADVVGSTSQLLNAVMKMDASTFIVATDNGIL
                     HRMRQMAPGKILIEAPTAGSSATCKSCAHCPWMAMNGLQGVVDCLEQGRGEIQVPEPT
                     RSQALGCIDRMLDFVARHPASIAPSVQGFVPQIGAA"
     gene            complement(4593..>4944)
                     /locus_tag="VFV25_04845"
     CDS             complement(4593..>4944)
                     /locus_tag="VFV25_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY1392677.1"
                     /translation="ARPVPLGVNLPQDKRLRSLCLAVLDDPTRHPKLADWAKDAAASE
                     RTVARLFRQELGTSFAQWRQQVILAKAVALAARKRPMSHIAAELGYASASAFTAMVKR
                     SVGEPPRRFFASAA"
BASE COUNT          758 a         1671 c         1682 g          833 t
ORIGIN      
        1 gattggagct cggccaggcc gagcgttcga aagggtgggc tgccatgagt cggtcctctg
       61 aggtgccgcc gtctgttgcg gcgttcacgg tgcttgggtc cagaaggcga gcgacctggg
      121 cagtgtaagc cggtgtttca ggacgtcgcg aagggtgcta ggcatgattt cacgctggcg
      181 cccggcatag ttaccccctg aggagggttt gcaacaggca cccccctcat tcgagtggga
      241 gggtcttgcc tgtatcgctg ccgtactgca ggcaggtcgt ttccggggcg gtcgatagaa
      301 tcgaccgatc gatcgctgct gcacctgcgg ccggcgacat gcctaggatc gcagcctctt
      361 atgcccgctc cgcgtccccc gacttcgcag caagcccccg tcgtcatcat cggtgccggc
      421 ctggccggcc tgaccgtggc cctgcatctg gccgaacagc atcccgtggt ggtgttggcc
      481 aagcgcaatc tcgacgaagg tgccacggcc tgggcccagg gcggcatcgt cggcgtgctg
      541 ggcgacgacg acagcatcga gtcccacgtg cgcgacacgc aggatgccgg cgccggcctg
      601 gtggacgagg agaccgcacg cttcatcgcc gagcacagcg ccgcggcgat cgaatggctg
      661 gtcgcgcgcg gcgtgccttt ctcgcccgat cccagcggcc cgctcggcct gcatctgacg
      721 cgggaaggcg ggcacgcggt gcgccgcatc gcccatgcgg ccgacgcgac gggcaaggcg
      781 atacacgacg tgctgctgcg cgaggtgcag cgccacccca acatcacgct gcgtgagcgc
      841 tggatggccg tggatctgat cacctcgcgc cacctcaagc gcgaggagcc ctcgcggtgc
      901 tacggcgtct atgcgctgga catcgattcc cagcgcgtcg agaccctgcc ggccgcggcg
      961 gtcgtgctgg ccacgggggg cgccggcaag gtctatcgct acaccaccaa ccccgacacc
     1021 tccacgggcg acggcattgc gatggcctgg cgcgcgggct gccgtgtcgg caacatggaa
     1081 ttcatccagt tccacccgac ctgcctgtac cacccccagg agcgcagctt cctgatcacc
     1141 gaggcgctgc gcggcgaggg aggccagctc aagctgccga gcctcggacc gggtgacccc
     1201 gggggctccc gcttcatgcc cgcgcacgac gagcgcgccg aactggcgcc gcgcgacatc
     1261 gtcgcccgcg ccatcgactt cgagatgaag aagcatggcc tggactatgt gctgctggat
     1321 gcgacccatc tgggcgagac cttcctgaag gagcatttcc cgaccatcta cgcgcgctgc
     1381 ctggccctgg gcctggacat cgcgaagcag cccatccccg tggtgccggc ggcgcactac
     1441 acctgcggcg gcgtggtcac cgacctggac gggcggaccg acctgcccgg gctctatgcc
     1501 gtgggcgaaa ccacctacac cggcctgcac ggcgccaacc ggctggccag caactcgctg
     1561 ctcgaatgcg tggtgctcgg ccgcacctgc gcccagtacg tgctggcgca tcgcgcctcg
     1621 gtggaagtcg atctgccggc ctgggacgag agccaggtcg aggatgccga cgagcaggtc
     1681 gtcatctcgc acaactggga cgaactgcgc ctgctgatgt ggaactacgt cggcatcgtg
     1741 cgcaccacca agcggctgga gcgggccttg caccgcatca agctgctgcg caaggagatc
     1801 gacgagtact acgcggcctt ccgcgtcagc cgcgacctgc tggaactgcg caatctggtg
     1861 gaatgcgccg aactcatcgt gcgctcggcg ctgatgcgcc aggaaagccg cggcctgcac
     1921 tacagccgcg attttccgta tgccttgccg gtgagttttc ccaccgtgct ggtgcgtgcg
     1981 gccaggagcg gcgccaacgg caagggcgaa accaagcaca aggctgtggg cctgagcggg
     2041 tagggcaccc ctcccggaat gggagcgcta gcggcgcgcc cgcatcgcga cgcgtgcctg
     2101 cacgaattcg agcgcgtgct gcacgacctc ggcgatggtc ctttcgttgt ccacgcgttc
     2161 cttgtcgggc atgtagaagg ccagatccac agagtggcgg atatagcggg tgggtaagcg
     2221 gttcaaggcg acgcaggctg catcggccag caattcagcg ccgagatgtg ggtagttcgg
     2281 cgcggcggcg agtatcgcgt cgaacaccgg ctgctcgtag aagttctgga tggattcgaa
     2341 gctctggacc atggccgccc cttgaccgtc cgctggagat gccaggtttt cggcagggcg
     2401 ccggcggaat tgagcggcgg ccttgcaact cgccaggcag cgtgatttgt tgcgcgccta
     2461 caggcgctcc agcacccgca tcgaatagtc caccgccttg acgtccttgg tgagccggcc
     2521 gattgaaatg cggtcgacgc ctgtggccgc gatctcgcgc aattgctcca gtgcgatgcc
     2581 gcccgagacc tcgagcaggg cacggcccgc gttcaccttg accgcctcgc gcatctgcgc
     2641 atagctgaag ttgtcgagca gcacgctggt ggcaccggcc gccagcgcct cgcgcaattg
     2701 ctccagggtc tcgacctcga tctgtatgcc cacctcggcg tccaaggcct gcgcggcttt
     2761 cagagcctgg gtcacgccgc cggccgccgc gatgtggttt tccttgatca ggatgccgtc
     2821 atagagcgcc agacgctggt tctgcccgcc gccgacgcgc accgcgtact tttgcgccag
     2881 gcgcaggccg ggcaaggtct tgcgggtgtc gaggatggcg cagcccctgg ggttggtcga
     2941 ggcgcccgcc acggcatcca catggcggcg cgttgtgctg gctacgccag acagcagctg
     3001 caggaagttc agcgccgggc gctcggcggc gagcagggcg cgcgcctggc cctcgatgtg
     3061 gcagaccagg ctgtcggccg tcatgtgtgc gccttcggca tagtgccaat cgatgcgcgc
     3121 ttcggcatcc agtgcatgca cgcaggcctc gaaccaatcg cgcccgcaca gcaccgcctc
     3181 ctcgcgcacc agcacgcggg cgcgcacgcg ctcggcctcg ggcaacagga ggccggtcca
     3241 gtcgcagcgg ccgaggtctt cgaacagggc ctcgcgcaca ttgcgcgcgc gggcttcttc
     3301 cagggtttcg ttgttcagca tagtgactag tgatcggtga ctggtgactc gtgatttctt
     3361 ggctgttcac tggtcaccgg tcacaggtca ccagtcgctg cctcatgcgg cgccgatctg
     3421 gggcacgaag ccctgcacgg acggggcgat gctggcggga tgcctggcca cgaagtccag
     3481 catgcggtcg atgcagccca gggcctggct gcgcgtgggc tcgggcacct ggatctcgcc
     3541 tctgccttgc tccaggcagt ccaccacgcc ttgcagcccg ttcatcgcca tccaggggca
     3601 atgggcgcag ctcttgcagg tggcgctgct gccggcggtc ggcgcctcga tcaggatctt
     3661 gcctggcgcc atctggcgca tgcggtgcag gatgccgttg tcggtggcga cgatgaaggt
     3721 gctcgcgtcc atcttcatca cggcattgag cagctgcgag gtcgagccga cgacgtcggc
     3781 ctgctcgacc acgctcttgg gcgactcggg gtggaccagc actttcgctg caggatgctc
     3841 gcggcgcagc agctccagct cgatgccctt gaactcgtcg tgcacgatgc actcgccgtt
     3901 ccacatcagc atgtcggcgc cggtgttctc ctcgatgtag cggcccaggt ggcgatcggg
     3961 cgcccacagc accttctggc cttgcgccga aagctgctgg acgatggcca gcgcgcagga
     4021 gctggtgacc atccagtcgg cgcgtgcctt caccgccgcg ctggtgttgg catacacgac
     4081 cactttgcgg tccgggtggg cgtcgcagaa ggcggcgaat tcgtcgggcg ggcagccgag
     4141 gtccagcgag caattggctt ccaggtcggg catcagcacg cgcttgtgcg gcgacaggat
     4201 cttggccgtc tcgcccatga agcgcacgcc ggccacgacc agggtctggc tctcgtggtc
     4261 gcggccgaag cgggccatct ccagcgaatc ggacacgcag ccgccggtct ccagcgccag
     4321 gtcctggatg tcgccgtcca cgtagtaatg ggccaccagc acggccttgt ggcgcttgag
     4381 cagggcgccg gcacgcgcct tccattcacg gcgcgcctgt ggtcccagag gctcaggcac
     4441 cttggcccag gcatgggcgg tgcaggacac gccctgggcg tcctgcttcg tgtagtcgaa
     4501 cagcacttgg ctcatttcat cgaatccttg gggccgcggc cccgacacca aaatggtagc
     4561 ccagcggctg ttcccgcatc ggctgtgcag gctcaggccg ccgatgcgaa gaagcgccgg
     4621 ggcggctcgc cgaccgagcg cttcaccatg gccgtaaaag cgctcgcact ggcatagccc
     4681 agctcggcgg cgatgtggct catgggccgc ttgcgcgccg ccagcgcaac ggccttggcg
     4741 agaatcacct gctggcgcca ttgcgcgaag ctggtgccca gctcctggcg gaacagccgc
     4801 gccaccgtgc gctcgctggc ggcggcgtcc ttggcccagt cggccagctt cggatgccgg
     4861 gtggggtcgt ccagcacggc caggcacagg ctgcgcaagc gcttgtcctg cggcaggttc
     4921 acccccagcg gcacggggcg ggcg
//
LOCUS       DASUCY010000254         3075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_615683, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000254 DASUCY010000000
VERSION     DASUCY010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615683"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VFV25_04850"
     CDS             <1..502
                     /locus_tag="VFV25_04850"
                     /inference="COORDINATES: protein motif:HMM:NF021158.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HEY1392678.1"
                     /translation="LREGLSLWGHGPQLPGRPATAMAAAPATTAVTASSGGASAAETA
                     GTNSACQISGSVSYRERMALLPGDELLVQLLDASQPDAPPRVLSEQRLSNPGQVPLHF
                     LLSCDAKLPGQQANFLIAARILRDGRPLFSGSMPLPPGDQTKDLELMLSQMRGLPAKP
                     NAENSR"
     gene            512..1228
                     /locus_tag="VFV25_04855"
     CDS             512..1228
                     /locus_tag="VFV25_04855"
                     /inference="COORDINATES: protein motif:HMM:NF018745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HEY1392679.1"
                     /translation="MDSAKPPAVAESDHASATTSSLLRLRRRGARLKRRLGLAFALGL
                     AGMLLMRLAWPWTEAALAGWVVFVVADLALAWRELASLTAAQTRASVESNDQSSPVLR
                     ALVLLGCAASLGAIALLLFGSHQLPLVQRLSAAALAMLAIGASWLMIHVHFAMRYAHR
                     YYRHPAASPAPLQFPGAEPPDFLDFFYFSFVIGMTSQVSDVTIQERGMRRSVLVHSLL
                     SFGFNLMILALAINALASAF"
     gene            complement(1246..>3075)
                     /locus_tag="VFV25_04860"
     CDS             complement(1246..>3075)
                     /locus_tag="VFV25_04860"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018423005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY1392680.1"
                     /translation="PAEALPEHDALRPGTRLGEFEIERVLGVGGFGIVYLAFDHALER
                     RVALKEYMPAALAARGRGAMVSIRSSSHAETFGVGLRSFVNEAKLLARFDHPSLVKVY
                     RFWEDNGTAYMVMPYYEGRTLRETRKAMSAPPDEAWLRALLEPLLGALEVLHAEQVYH
                     RDIAPDNILLLTDGRPVLLDFGAARRVIGDRTQTLTAILKPNFAPIEQYAETAGLRQG
                     PWTDLYSLGAVVHFCLSGQPPTPAAARAVHDELPTVRTLAATLDRQFHLSYGDAFVDA
                     IDACLAVRPQGRPASVAALRAALRGEASIAYPPTQPYARDDDAHRDSEPGFAATQLEP
                     RGRQEDADATIAVPRAVLAGGRREPTLGAPVSPPPARSAKSTKSAARQAPRKSRAAQL
                     ASLGLAVAAIVGAAWFLSGRLGVAPLRVSQAASAPAAAASVSAPAVAPPAASAAAEQA
                     VAAASSQSGTTAAPADAAAQPPLSMASAAAADLTSEADLAALAAGGGGAAAAASAAPS
                     KPKPRQPARRDDSGALRPRPGSLAATAAPVAEPQGVAQPLQAAAPAAQQAPHSPREAC
                     GKRVFLAMYYCIKEQCEKPEFAQHPQCRHQRAQDEANRPTGEQ"
BASE COUNT          421 a         1070 c         1112 g          472 t
ORIGIN      
        1 gctgcgcgaa ggcctgagcc tgtggggcca tgggccccag ctgcccggcc gaccggccac
       61 agccatggcg gcggcccctg caacaacggc cgtcaccgcg agcagcggcg gcgcctcggc
      121 tgcagagacc gccggaacca acagcgcctg ccagatcagc ggcagcgtca gctatcgcga
      181 gcgcatggcc ctgttgcccg gcgacgagct gctggtgcaa ttgctcgatg cctcgcagcc
      241 cgacgcgcca cccagggtgt tgagcgagca gcggctgtcc aatccgggtc aggttccgct
      301 acattttttg ttgtcatgtg acgcaaaatt gcctgggcaa caggcgaatt ttctgattgc
      361 agctcgcatc ctgcgcgatg gcaggccgct gttttccggc agcatgccgt tgccgcctgg
      421 ggatcagacc aaggacctgg agctgatgct gtcgcagatg cgcggcctgc ccgccaagcc
      481 caacgccgaa aacagccgct gaaaagtaag catggacagc gccaagccgc ctgccgttgc
      541 cgagtccgat cacgcaagcg cgacgacgag ttcgctgctc cgcctgcgtc ggcgcggcgc
      601 gcggctgaag cgccgcttgg ggctggcctt tgcgctcggc ctcgccggca tgctgctgat
      661 gcgcctggcc tggccctgga cggaggcggc gctggccggt tgggtggtct tcgtcgtggc
      721 cgacctggcg ctggcctggc gcgagttggc gtcgctgacg gcggcgcaga cccgcgccag
      781 cgtcgagagc aacgaccaaa gcagcccggt gctgcgcgcg ctggtgctgc tgggctgtgc
      841 ggccagcctg ggggcgatcg cgctgctgct gttcggctcg caccagctac cgctggtgca
      901 gcgcctgtcg gccgccgcgc tggcgatgct cgcgatcggc gcgtcgtggc tgatgatcca
      961 tgtgcacttc gcgatgcgct atgcgcaccg ctactaccgg catcccgccg ccagccccgc
     1021 gccgctgcag ttcccgggcg ccgagccgcc ggacttcctg gattttttct acttctcctt
     1081 cgtgatcggc atgacctcgc aggtctccga cgtcacgatc caggagcgcg gcatgcgccg
     1141 cagcgtgctg gtgcacagcc tgctgtcctt cggtttcaac ctgatgatcc tggcgctggc
     1201 gatcaacgcc ctggcctcgg cgttctgatg cctcttagct gagggctatt gctcgccggt
     1261 cgggcggttg gcctcatcct gggcccgctg atgcctgcat tgcggatgct gggcgaattc
     1321 cggcttctcg cactgctcct tgatgcagta atacatcgcg aggaagacgc gcttgccgca
     1381 ggcctcgcgc ggactgtgcg gggcctgctg cgcggcagga gcggcagcct gtaagggctg
     1441 ggcaacgccc tgcggttcgg ccaccggcgc cgccgtcgcg gccaggctgc cgggccgggg
     1501 gcgcaaggcg ccgctgtcgt cgcgccgcgc cggctgcctg ggtttgggct tggagggcgc
     1561 ggcggatgca gcggcagcgg ccccaccgcc gcccgccgcc agtgcggcca gatcggcttc
     1621 ggaagtcaga tcggccgctg cggccgaggc catgctgagc ggcggctggg ccgccgcgtc
     1681 tgccggcgct gccgtcgtac cggactgcga tgacgccgcg gcgaccgcct gttctgccgc
     1741 ggccgaggcc gccggcggcg ccaccgccgg tgcggacacc gatgccgccg cggccggcgc
     1801 agacgcggcc tgtgaaacgc gcagcggtgc cacgcccagc cttccgctca ggaaccaggc
     1861 cgcaccgacg atcgccgcca ccgccagccc cagcgacgcg agctgagctg cgcgcgactt
     1921 gcgcggcgcc tgccgggccg ccgatttggt cgatttggcc gatcgcgcgg gcggcggtga
     1981 tacgggcgcg cccaaggtgg gctcgcgcct gccgccggcc agcacggcgc gcggcacggc
     2041 gatcgtagcg tcggcatctt cctggcggcc gcgcggctcc agctgcgtgg cggcgaagcc
     2101 cggctcgctg tcgcgatgcg catcatcgtc acgcgcgtaa ggctgggtcg gcggataagc
     2161 gatgctcgcc tcgccgcgca aggcggcgcg cagggccgcg acgctggccg gccggccctg
     2221 gggacgcacg gccaggcagg catcgatggc atcgacaaaa gcgtcgccat agctcaggtg
     2281 gaactgccgg tccagcgtgg cggccagcgt gcgcacggtc ggcaattcgt cgtgtacggc
     2341 acgcgccgcc gccggcgtgg ggggctggcc gctcaggcag aaatgcacga ccgcgcccag
     2401 cgaatagaga tcggtccagg gcccctggcg cagaccggcg gtttcggcgt attgctcgat
     2461 gggcgcgaag ttgggcttga ggatggcggt gagcgtctgc gtgcggtcgc cgatcacgcg
     2521 gcgcgcggcg ccgaagtcca gcagcaccgg ccggccgtcg gtcagcagca ggatgttgtc
     2581 gggcgcgatg tcgcggtggt agacctgctc ggcatgcaac acctcgagcg cgcccagcag
     2641 cggctccagc aatgcacgca gccaggcctc gtcgggcgga gcgctcatcg ccttgcgcgt
     2701 ctcgcgcagc gtgcgcccct cgtaataggg catcaccatg taggcggtgc cgttgtcttc
     2761 ccagaagcgg tagaccttga ccagcgaagg atggtcgaaa cgggccagca gcttggcctc
     2821 gttgacgaag gagcgcagcc cgacgccgaa ggtctcggcg tgggaggacg agcgtatcga
     2881 caccatcgcc ccgcgcccgc gcgcggccag cgcggccggc atgtattcct tcagcgcgac
     2941 gcggcgctcc agcgcgtggt cgaaggcgag atagacgatg ccgaagccgc ccacgcctag
     3001 cacacgctcg atctcgaact cgcccagccg ggtgccgggg cgcagggcat cgtgttcggg
     3061 cagcgcttcg gcggg
//
LOCUS       DASUCY010000255         2469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1177899, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000255 DASUCY010000000
VERSION     DASUCY010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177899"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VFV25_04865"
     CDS             complement(<1..252)
                     /locus_tag="VFV25_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392681.1"
                     /translation="MLAPAIFKRAAGAAAMVATLGLAGCGGGAYVDVGGGPSVGPPPT
                     ITLAITVPSPALPGDAVTLLADPQASNGIDYVNFYRIDPG"
     gene            550..>2469
                     /gene="uvrA"
                     /locus_tag="VFV25_04870"
     CDS             550..>2469
                     /gene="uvrA"
                     /locus_tag="VFV25_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEY1392682.1"
                     /translation="MIRVRGARTHNLKNIDLDIPRYKLVVITGLSGSGKSSLAFDTLY
                     AEGQRRYVESLSAYARQFLQLMDKPDVDVIEGLSPAISIEQKATSHNPRSTVGTVTEI
                     HDYLRLLYARAGTPYCPDHGLALEAQSVSQMVDATLALPEDARLMVLAPVLRERKGEH
                     AELFEDMQSRGYVRFRVDGQIVEATELPKLKKTEKHDIDIVVDRLKSRPDMKQRLAES
                     FEAALRMADGRAIALEMDSGREHLFSSKFACPICSYSLAELEPRLFSFNSPIGACPSC
                     DGLGEVVVFDPARVVAFPSLSLASGAVKGWDRRNAYTFSLLESVAKHYGFDIDMPFEQ
                     LAAQHQQHLLYGSGTEEIPFVYAAEGASGRRRTVKRWHPFEGIIANFERRFRETDSIA
                     VREDLARYQSNKPCPECQGTRLRREARFVKLVDAQDGSARPIYEISHVTLREALQIFE
                     QLQLKGAKAEIAGKVVREIASRLKFLNDVGLSYLSLDRSADTLSGGEAQRIRLASQIG
                     SGLTGVMYVLDEPSIGLHQRDNDRLIGTLKHLRDLGNSVLVVEHDEDMIRAADHVVDM
                     GPGAGVHGGRVLVQGTPEQIEQSPASLTGEYLSGKRRIEVPAQRTGPDDERWLRIVNA
                     TGNNLKNVSADIPVGL"
BASE COUNT          425 a          839 c          823 g          382 t
ORIGIN      
        1 gccggggtcg atgcgataga aattcacata gtcgatgccg ttgctggcct gggggtcggc
       61 gagcagggtc acggcatcgc caggcagggc gggcgagggc acggtgatcg ccagcgtgat
      121 ggtgggcgga ggcccgacgg aaggcccgcc gccgacatcg acataggcgc cacctccgca
      181 gccggccagg cccagagtgg ccaccatcgc ggcggcaccg gccgcacgtt tgaagatcgc
      241 tggagcaagc atggctgcgt cctctctctg tatctgtatc tgttgcgctt gtgcaagggg
      301 acgatcccct cgcagcatcc gtagcgatac aacgcttgtc cgcatgcaac ggttgtccgg
      361 cgcctgcttc cttctgtaac cgccggctga cgacaggccc gcgcaagggc gaggcctgct
      421 ccgatcggcg cggctggtta tcatggtcgg ttgcctcacg ttcacccgcg ttcacccacc
      481 acgatggccc gccgccccga aactgcgctc tccgcgcccg tcgaaacgac cgagaacagc
      541 ggcgcaccga tgatccgggt tcgcggcgca cgcacccaca acctcaagaa catcgatctc
      601 gacatcccgc gctacaagct ggtggtgatc accgggctat cgggctcggg caagtccagc
      661 ctggccttcg acacgctgta tgccgaaggc cagcgccgct atgtagagag cctgtcggcc
      721 tatgcgcggc agttcctgca gctgatggac aagcccgatg tcgatgtgat cgaaggcctg
      781 tcgccggcca tctccatcga gcagaaggcc accagccaca acccgcgctc caccgtgggc
      841 acggtgaccg agatccacga ctacctgcgc ctgctctatg cacgcgccgg cacgccctat
      901 tgccccgacc atggtctggc gctcgaggcg cagagcgtga gccagatggt cgacgcgacg
      961 ctggccctgc ccgaggacgc gcgcctgatg gtgctggccc cggtgctgcg cgagcgcaag
     1021 ggcgagcatg ccgagctgtt tgaggacatg cagtcgcgcg gctatgtgcg ctttcgcgtc
     1081 gacggccaga tcgtcgaggc caccgagctg cccaagctga agaagaccga gaagcacgac
     1141 atcgacatcg tcgtcgaccg gctcaagtcg cgcccagaca tgaagcaacg cctcgcggaa
     1201 agcttcgagg ccgcgctacg catggccgac ggccgcgcca ttgcactgga gatggactcg
     1261 ggccgggagc atctgttcag cagcaagttc gcgtgcccga tctgcagcta ttcgctggcc
     1321 gagctggagc cgcggctgtt ttccttcaac tcgccgatcg gcgcctgccc gagctgcgac
     1381 ggcctgggcg aggtggtggt gttcgatccg gcgcgcgtcg tcgccttccc gtcgctcagc
     1441 ctggccagcg gcgcggtgaa gggctgggac cggcgcaatg cctacacctt ctcgctgctg
     1501 gagagcgtcg ccaagcatta cggcttcgac atcgacatgc ccttcgagca gctggctgcg
     1561 cagcaccagc agcatctgct ctacggctcg ggcacggagg agatcccttt cgtctacgcg
     1621 gccgagggcg caagcgggcg caggcgcacg gtcaagcgct ggcacccctt cgaaggcatc
     1681 atcgccaact tcgagcggcg cttccgcgag accgactcga tcgcggtgcg cgaggacctg
     1741 gcgcgctacc agagcaacaa gccttgcccc gaatgccagg gcacgcggct gcgccgcgag
     1801 gcgcgcttcg tgaagctggt cgacgcgcag gacggcagcg cgcggccgat ctacgagatc
     1861 tcgcatgtca cgctgcgcga ggcgctgcag atcttcgagc agctgcagct caagggcgcc
     1921 aaggccgaga tcgccggcaa ggtggtgcgc gagatcgcct cgcggctgaa gttcctcaac
     1981 gacgtgggcc ttagctatct gagcctggac cgcagcgccg acacgctctc gggcggcgag
     2041 gcccagcgca tccgcctcgc ctcgcagatc ggctcgggcc tgaccggagt gatgtatgtg
     2101 ctggacgagc ccagcatcgg cctgcaccag cgcgataacg atcggctgat cggcacgctc
     2161 aagcacctgc gcgacctggg caactcggta ctggtggtgg agcacgacga ggacatgatc
     2221 cgcgccgccg accatgtggt cgacatgggg ccgggcgccg gcgtgcatgg cgggcgcgtg
     2281 ctggtgcagg gcacgcccga gcagatcgag cagtcacccg cgtcgctcac tggcgaatac
     2341 ctctcgggca agcgccgcat cgaggtgccc gcgcagcgca ccggccccga tgacgagcgt
     2401 tggctgcgca tcgtcaacgc caccggcaac aacctgaaga acgtgtcggc cgacatcccg
     2461 gtcgggctg
//
LOCUS       DASUCY010000256         2134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_985305, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000256 DASUCY010000000
VERSION     DASUCY010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_985305"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1950
                     /locus_tag="VFV25_04875"
     CDS             <1..1950
                     /locus_tag="VFV25_04875"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEY1392683.1"
                     /translation="DPKQSIYRFRRAEPRVFAAAQDFVRQALGGSLLACDHTWRNAPE
                     VLGVLNQVFEAAQAERSYQGFRAHTTAASHAGAVLVLPPVPRPEKTATAQAGMVWRDS
                     LTVPRIAAEEALRLAEARHVAAAIAELIGQGWPVGEIHVLARKRAHLRVLGEALRELH
                     LPFIAPEETALMAAIEVRDLVALLDALVSPRHELALAQALKSPIFGASDDDLMRLALL
                     ARGNGSGWWAALVRHDHESAELARARELLAAWQQAAKRLPPHDLLNRIVLEGEVKERI
                     AAAVPASQRSAALEHVDALLAQALLLDGARYATPYNFVRSLKRRLVEMPPRPRGDAVQ
                     LLTVHGAKGLEARAVFVMDADAAPSHSETTALLVDWPVESAHPRCCAFLASFKHAPLA
                     LRPLIAAEREAQQREELNALYVAVTRAKDRLIFSLTEPHRRGAEDAAPNWWQRLEKLG
                     LPTWEPAAAAPDARSEAAWIELPSLPLAQPRQAGLFDGAEGLPDSEAARLGRAVHRVL
                     EWAVRRPPERRAAELDACIATVGAEFRLQAAQKGSVRALSEAVLAGAEVQRFLDPRQS
                     AWSGNEVPLSWRSEQIRLDRLVAFDTETGREWWVLDYKLQHAPEQLAGYRAQLENYRR
                     AVQAAQPGERVRAAFITGAGRLVEL"
BASE COUNT          318 a          751 c          759 g          306 t
ORIGIN      
        1 gaccccaagc agagcatcta ccgcttccgt cgcgccgagc cgcgcgtctt cgccgcggct
       61 caggacttcg tgcgccaggc cctgggcggc agcctgctgg cctgcgacca cacctggcgc
      121 aatgcccccg aggtgctggg cgtgctcaac caggtgttcg aggcggcaca ggccgaacgc
      181 agctaccagg gctttcgcgc ccacacgacg gcggcctccc atgccggcgc cgtgctcgtg
      241 ctgccgccgg tgccgcgtcc cgagaagacc gccacagcgc aagccggaat ggtctggcgc
      301 gacagcctca cggtgccgcg catcgcggcc gaggaagcct tacgcttggc cgaggccagg
      361 catgtcgccg cggccatcgc ggagctgatc ggccagggat ggccggtcgg ggagatccat
      421 gtgctggcgc gcaagcgcgc ccatcttcgc gtgctgggcg aggccttgcg cgagctgcat
      481 ctgcccttca tcgcgccgga agaaacggcg ttgatggccg cgatcgaagt gcgcgacctc
      541 gtggccctgc tggatgcgct ggtgtcgccg cgccatgagc tggccctggc ccaggcgctc
      601 aagagcccga tcttcggcgc cagcgacgac gacctgatgc gcctggcgct gctggcccgc
      661 gggaacggca gcggctggtg ggccgcgctc gtgcgacacg atcatgaaag cgccgagctg
      721 gcgcgagccc gcgagctgct ggctgcctgg cagcaagccg ccaaacgcct gccgccgcac
      781 gacctgctca atcgcatcgt cctggaaggc gaggtgaagg agcgcatcgc cgccgccgtg
      841 ccggccagcc agcgcagcgc agcgctcgag catgtcgatg ccctgttggc gcaggccctg
      901 ctgctggacg gtgcgcgcta tgcgacgccc tacaacttcg tgcgctctct caagcggcgc
      961 ctggtcgaga tgccgccgcg cccgcgcggc gatgcggtgc agctgctcac cgtgcacggt
     1021 gccaagggcc tcgaggcccg cgcggtcttc gtgatggatg ccgacgccgc gccgtcccac
     1081 agcgagacga ctgcgttgct ggtggactgg ccggtggaat ccgcgcaccc gcgctgctgc
     1141 gccttcctgg cttccttcaa gcatgcgccg ctcgcgctca ggccactcat cgcggccgag
     1201 cgcgaagccc agcaacgcga ggagctgaac gccctctatg tcgccgtcac gcgcgccaag
     1261 gacaggctga tcttcagcct caccgagccg catcgccgcg gcgccgagga cgcggcgccg
     1321 aactggtggc agcggctgga gaagctgggc ctgccgacct gggagcctgc cgcagcagcg
     1381 ccggatgccc gcagcgaggc cgcatggata gaactgccaa gcttgcccct tgcgcagccg
     1441 cgccaggcgg ggctcttcga tggtgcagaa ggcctgcccg acagcgaggc ggcgaggcta
     1501 ggccgcgccg ttcatcgcgt gctcgaatgg gcggtgcgca ggccgccgga gcggcgtgcc
     1561 gccgaactcg atgcctgcat cgccacggtg ggcgcggagt tccgcctgca agccgcacag
     1621 aagggcagcg tcagagcgct cagcgaagcg gtgctggccg gcgccgaggt tcaacgcttc
     1681 ctcgatcccc ggcagtcggc ctggtcgggc aatgaagtgc cgctgtcctg gcggagcgag
     1741 cagatccggc tggatcgcct ggtcgccttc gataccgaga cggggcggga gtggtgggtg
     1801 ctcgactaca agctgcagca cgcacccgag cagctggccg gctatcgcgc gcaactggag
     1861 aactaccgcc gcgccgtgca ggccgcgcag ccgggcgagc gcgtgcgggc cgccttcatc
     1921 acgggcgcgg gtcggctggt ggagctgtag cctgctaccc agcttttctt catagccacg
     1981 aaacagaacc gagaaccgat gagtcccagc cttcactaca gccacgaccc gtgcggctga
     2041 tgctacgccg ccgatcgccg cggctgccga tgcggtctcg cagggccagg caggtcctgg
     2101 gtgccatgcg gatggtcgtg tggtggggtg acga
//
LOCUS       DASUCY010000257         4591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_37463, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000257 DASUCY010000000
VERSION     DASUCY010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4591
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37463"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..119
                     /locus_tag="VFV25_04880"
     tRNA            43..119
                     /locus_tag="VFV25_04880"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:77..79,aa:Gln,seq:ttg)
     gene            243..1211
                     /locus_tag="VFV25_04885"
     CDS             243..1211
                     /locus_tag="VFV25_04885"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEY1392684.1"
                     /translation="MLADTVLLFTGNANPALAQEIGTHLGIGLGKAKVGRFSDGEVDV
                     EIQQNVRAREVFVVQPTCAPDTNEHLMELFIMVDALKRASSRRITAVIPYFGYARQDR
                     RPRSTRVPISAKVVANMLEAVGVNRLLTMDLHADQIQGFFDIPVDNIYASPVLLADLK
                     ARNYDDLMVVSPDVGGVVRARAMAKQLGCELAIIDKRRPKANVSEVMHVIGDIEGRNC
                     VIMDDMIDTAGTLVKAAEVLKERGAKSVFAYCTHGVFSGPAIERIKSSQLDEVVITNT
                     IPLTGEAKATTKIRQLSVAFLFAETIRRISDGESVTSLFSEQNNNF"
     gene            1288..1938
                     /locus_tag="VFV25_04890"
     CDS             1288..1938
                     /locus_tag="VFV25_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEY1392685.1"
                     /translation="MKFVAFPRELQGTGASRRLRNAGKVPGIVYGAGEPARIELDHNA
                     LFHALKKEAFHSSVLEMELNGNVQQVLLRDVQMHAWKQQVLHVDFQRVDATTRITKKV
                     PLHFVNEENSQAIKLDQCLISKVANELEIECLASQLPEFLTVDLGGLSKGQSLHVSDL
                     KLGAGVKVVLHGKKDPVIAAVVAQAAEEEAPAEAAPVVAAAPAPSKGKKDEKQNKR"
     gene            2033..2656
                     /gene="pth"
                     /locus_tag="VFV25_04895"
     CDS             2033..2656
                     /gene="pth"
                     /locus_tag="VFV25_04895"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEY1392686.1"
                     /translation="MIRLFVGLGNPGAEYEATRHNAGFWWVDAAARKLGATLLPDRSY
                     HGLVARVNRPEGPVWLLEPMTFMNLSGKSVAALARFFKIAPGEILVAHDELDLPPGQV
                     KLKLGGGHAGHNGLRDIHAQLGSADYWRLRLGIGHPGVKAEVVNYVLRKPAPEQREAI
                     EASVDRTLGALDALLAGDMERATLQIHTSKPPRPKPPRPMATEEASD"
     gene            2689..3156
                     /locus_tag="VFV25_04900"
     CDS             2689..3156
                     /locus_tag="VFV25_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392687.1"
                     /translation="MKHRKKLKAALFGSLLAAVATAQAQAVYRCGPDGRSYSREPCAG
                     GKAVDVSDPRSPEQCQAAEQMARDDARRAREMAAENRQRQRKQQPAAAAVIALEGKPA
                     LSRKDEPRAQPHRHGKTHKPKLGKPAKPEAFEALGPADSASQPPKKKRKPRAA"
     gene            complement(3153..3455)
                     /locus_tag="VFV25_04905"
     CDS             complement(3153..3455)
                     /locus_tag="VFV25_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HEY1392688.1"
                     /translation="MALMITDECINCDVCEPECPNQAISMGPDYYVIDPGRCTECVGH
                     FPEPQCVQVCPVSCIPINPAHVESREQLMAKYLLLQQAKEPAAASQPPSEAKGSAG"
     gene            complement(3597..4115)
                     /gene="coaD"
                     /locus_tag="VFV25_04910"
     CDS             complement(3597..4115)
                     /gene="coaD"
                     /locus_tag="VFV25_04910"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEY1392689.1"
                     /translation="MTSATRLTAVYPGTFDPMTLGHVDLMRRASGLFERLILAVAAGH
                     HKRTMFNTDERLAIAQEAAKPYPNVEVTAFRGLLRDFVVAHGGRVVVRGLRAVSDFEY
                     EFQMAGMNRQLMPDVETVFLTPGDRYQFVSGTFVREIATLGGDVSQFVAPFVLERLKE
                     RVAQNQAHPRSG"
     gene            complement(4112..>4591)
                     /locus_tag="VFV25_04915"
     CDS             complement(4112..>4591)
                     /locus_tag="VFV25_04915"
                     /inference="COORDINATES: protein motif:HMM:NF015559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HEY1392690.1"
                     /translation="DRGLEEAGIEGRIQADEVEVAGRGAAEVVMLEREPRLAALLRQT
                     QGRLRAAQVRIEVADALAWMRGAAPDHFDLICLDPPFDAGLFEAAIQAATPLLAPGGW
                     LYLEADRVYGDADLSGLVVHRHGKAGAVHYHLLQPTPPRHDNDDNNLHEAAPGVTTP"
BASE COUNT          782 a         1541 c         1538 g          730 t
ORIGIN      
        1 tttttttgag ggcatggctg cagttcgggc cgggtcctgt gtaggggagt cgccaagttg
       61 gttaaggcat cggattttga ttccgacatg cgagggttcg agtccttcct cccctgccaa
      121 acctcttctt ctttctgagc gagagttccc gggcccggct ggcggctttg tgatccggct
      181 ggcgcggggc ctgtcaaaac gaaggcctgg accgcgccaa cgtcagtacc gaggatagcc
      241 gcatgctcgc cgataccgtt ctgctcttca ccggcaacgc caatcccgcg ctcgcgcagg
      301 agatcggcac gcatctgggc attggcctcg gcaaggccaa ggtcggccgc ttctccgacg
      361 gcgaagtcga cgtcgagatc cagcagaacg tgcgcgcccg cgaagtcttc gtggtccagc
      421 ccacctgcgc gcccgacacc aacgagcacc tgatggagct cttcatcatg gtcgatgcgc
      481 tgaagcgggc ctcgtcccgg cgcatcaccg cggtgattcc ctatttcggc tatgcccgcc
      541 aggaccgccg cccgcgctcc acgcgcgtgc cgatcagcgc caaggtcgtg gccaacatgc
      601 tcgaagcggt cggcgtcaac cggctgctga ccatggacct tcatgccgac cagatccagg
      661 gcttcttcga catcccggtc gacaacatct acgcctcgcc ggtgctgctg gccgacctca
      721 aggccaggaa ctacgatgac ctgatggtcg tgtcgcccga tgtcggcggc gtggtgcgtg
      781 cccgcgccat ggccaagcag ctcggctgcg agctggccat catcgacaag cgccgcccca
      841 aggccaacgt gtccgaggtg atgcacgtga tcggcgacat cgaggggcgc aactgcgtga
      901 tcatggacga catgatcgac accgccggca cgctggtgaa ggccgccgag gtgctgaagg
      961 agcgcggcgc caagagcgtg ttcgcctact gcacccacgg cgtgttctcg ggcccggcca
     1021 tcgagcgtat caagagctcg cagctcgacg aggtggtgat caccaacacc atcccgctca
     1081 ccggagaggc caaggccacg accaagatcc gccagctctc ggtggccttc ctgttcgccg
     1141 agaccatccg tcgcatttcc gacggcgagt ccgtcacgtc gctgttttcg gagcagaaca
     1201 acaacttctg atccgttcgt tggccgccgc aacgcggtgg cttttttcgg cgcctggtcg
     1261 cgggcgcttt cctaatggag tctagaaatg aaattcgtcg catttccgcg cgaactgcag
     1321 gggaccggag cgagccgccg cctgcgcaat gccggcaagg tgccgggcat tgtctacggt
     1381 gccggcgagc cggccaggat cgagctcgat cacaacgcgc tcttccacgc gctgaagaag
     1441 gaagccttcc actcgtccgt tctcgagatg gaactcaacg gcaacgtcca gcaggtgctg
     1501 ctgcgcgacg tgcagatgca cgcctggaag cagcaggtgc tgcacgtgga cttccagcgc
     1561 gtcgatgcga ccacccgcat caccaagaag gtgccgctgc acttcgtcaa cgaagagaac
     1621 tcccaggcca tcaagctcga ccagtgcctg atctccaagg ttgccaatga gctcgagatc
     1681 gaatgcctgg cctcgcagct gcccgagttc ctgaccgtcg acctgggcgg cctctccaag
     1741 ggccagtcgc tgcacgtcag cgacctcaag ctcggcgccg gcgtcaaggt cgtgctgcac
     1801 ggcaagaagg acccggtgat cgctgccgtc gtggcgcagg ccgcagagga agaggctccg
     1861 gccgaagccg cgcccgtggt ggccgcagcg cctgccccga gcaagggcaa gaaggacgaa
     1921 aagcagaaca agcgctgatg cttcgcgggc actgcgcccg gttcgttcga cgagaagccc
     1981 cgccttgcgg ggctttttct tgggcgctgc ggcctcgcga taatcccggc ccatgattcg
     2041 actcttcgtg ggcctgggca acccgggcgc tgaatacgag gccacgcgcc acaacgcagg
     2101 cttctggtgg gtggacgcag cggcgcgcaa gctgggcgcc acgctgctgc ccgatcgcag
     2161 ctatcacggc ctggtcgcgc gcgtgaaccg tcccgagggg ccggtgtggc tgctggaacc
     2221 gatgaccttc atgaacctct cgggcaagtc ggtcgcggcg ctggcccgct tcttcaagat
     2281 cgcgcccggc gagatcctcg tggcccatga cgaactcgat ctgccgcccg gccaggtcaa
     2341 gctcaagctg ggcggcgggc atgcgggcca caacggcctg cgcgacatcc atgcccagct
     2401 cggcagcgcc gactactggc ggctgcgact gggcatcggc cacccgggcg tgaaggccga
     2461 ggtcgtcaac tacgtgctgc gcaagcccgc acccgagcag cgcgaggcga tcgaagccag
     2521 cgtcgaccgc acgctgggcg cgctcgacgc gctgctcgcc ggcgacatgg agcgcgcgac
     2581 cctgcagatc cacaccagca agccgccccg gcccaagccg ccgcggccca tggcgaccga
     2641 ggaggcttcc gactagcgtt tccgtctttc aacagggaga aaaaaacaat gaagcaccgg
     2701 aagaaattga aggccgctct gttcggcagc ttgctggcgg ccgtagccac ggcccaggcc
     2761 caggccgtct accgctgcgg gcccgatggc cgcagctaca gccgagagcc ctgcgccggt
     2821 ggcaaggccg tggacgtgag cgaccctcgc tcgcccgagc agtgccaggc cgccgagcag
     2881 atggcacgcg acgacgcgcg ccgcgcacgc gaaatggcgg ccgagaaccg gcagcgccag
     2941 cgcaagcagc agccggccgc ggccgcggtg attgcccttg agggcaagcc cgcgctgtcg
     3001 cgcaaggacg agccgcgtgc tcagccgcac cgccacggga agacgcacaa gcccaagctt
     3061 gggaagccgg ccaagccgga agccttcgag gcgctggggc cggcagacag cgcttcccag
     3121 ccgcccaaga agaagcgcaa gccgcgcgca gcctagcccg ccgaaccttt ggcctcagac
     3181 ggcggctgcg aagccgcagc gggctccttg gcctgctgca gcagcagata cttcgccatc
     3241 agctgctcgc gcgattcgac atgcgccggg ttgatcggga tgcagctcac cgggcagacc
     3301 tgcacgcact gcggctcggg aaaatggccc acgcattcgg tgcatcggcc ggggtcgatg
     3361 acatagtagt ccgggcccat ggagatggcc tggttggggc attcgggttc gcagacgtcg
     3421 cagttgatgc attcgtccgt gatcatcagc gccatgctgt tcaacctctc gatggctatg
     3481 gcgtctcaag tcttcccctt gagggggccg agcccggaca cggggagtcg tgcggactgt
     3541 acgtgcccgg cgaggagccg gaccgtcctc ggacggcgcg gcctgcaggg ccggcgtcag
     3601 ccgctccggg ggtgggcctg attctgcgcc acgcgctcct tgaggcgttc gagcacgaag
     3661 ggtgccacga actgggagac atcgccgccc agagtggcaa tctcgcgcac gaaggtgccg
     3721 gagacgaact ggtagcggtc gccgggcgtg aggaagacgg tctccacatc cggcatcagt
     3781 tgccggttca tgccggccat ctggaattcg tattcgaagt cgctcaccgc gcgcaggccg
     3841 cgcaccacca cgcgcccgcc gtgcgcgacg acgaagtcgc gcagcaggcc gcggaaggcg
     3901 gtcacctcga cattggggta gggcttggcc gcttcctgcg cgatcgccag ccgttcgtcg
     3961 gtgttgaaca tggtgcgctt gtggtggccg gcagccacgg cgaggatgag ccgctcgaac
     4021 aggccgctgg cgcggcgcat caagtccaca tggcccagcg tcatcggatc gaaggtgccg
     4081 gggtagacgg cggtgagtcg ggttgctgag gtcatggggt cgtcacgcct ggggcggcct
     4141 cgtgcaggtt attgtcgtcg ttgtcgtgtc gcggcggcgt tggctgcagc agatggtagt
     4201 gcacggcgcc ggccttgcca tgtcggtgca cgacgaggcc gctgaggtcc gcatcgccgt
     4261 agacccgatc ggcctccagg tagagccagc cgcccggcgc gagcaagggc gtggcggcct
     4321 ggatggcggc ctcgaacagg cctgcatcga agggggggtc gaggcagatg aggtcgaagt
     4381 gatcaggtgc ggcgccacgc atccaggcca gcgcgtcggc gacttcgatg cgtacctggg
     4441 ccgctcgcag cctgccttgc gtctggcgca gcagcgcggc cagtctgggc tcgcgctcca
     4501 gcatcaccac ctcggccgcg ccgcgcccgg ccacttcgac ctcgtctgcc tggatccgcc
     4561 cttcgatgcc ggcctcttcg aggccgcgat c
//
LOCUS       DASUCY010000258         2017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1547597, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000258 DASUCY010000000
VERSION     DASUCY010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1547597"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VFV25_04920"
     CDS             <1..239
                     /locus_tag="VFV25_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY1392691.1"
                     /translation="TIAPTASLFIGAVKDTSLVAIVNLYDLTGTLRLAMGDSSWRPFF
                     VEMYLVVSLIYLAMGFSIAKYGRYLERRYALITK"
     gene            236..976
                     /locus_tag="VFV25_04925"
     CDS             236..976
                     /locus_tag="VFV25_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEY1392692.1"
                     /translation="MNSEAFLTINGVHKWFGAFHALDDCSLDVARGEKIVVCGPSGSG
                     KSTLIRCINRIEHHDAGRIAFNGTELDDGRPAVQARKEIGMVFQQFNLFPHLTVLDNV
                     MLAPTLVKRQDAHSAREEALALLKRVRIVEQAGKFPGQLSGGQQQRVAIARALAMQPQ
                     LMLFDEPTSALDPEMVGEVLDVMRELAQDGMTMIVVTHEMGFARQVADRVVFMEAGRI
                     LEIQPPELFFDRPQSERTRAFLGKILSH"
     gene            988..1938
                     /locus_tag="VFV25_04930"
     CDS             988..1938
                     /locus_tag="VFV25_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEY1392693.1"
                     /translation="MKRPYVLALHGGAGTIAAGSAADEQPYREALLFSLAAGEAVLAA
                     GGSALDAVVATVVALENCPLFNAGHGAVFTADALHELDASVMDGSSLGAGAVAGARQI
                     RNPVIAAREVLRQGRSVLLGGEGADRFARERGLEMVDPSYFSTPHRLEQLRAVQARAA
                     DLQTLDHDSQAMATEGEGRYGTVGAVALDRDGHLAAATSTGGMTNKQPGRIGDTPVVG
                     AGVYANDATCAVSGTGTGEHFLRACAGHDVHARMAYLGQSLDQAVEATLHQGIEPLGG
                     KGGLIAIDRRGQLSLRFNSTGMYRAWVREGAVRRAAVFAD"
BASE COUNT          325 a          662 c          674 g          356 t
ORIGIN      
        1 tcaccatcgc gcccacagca agcctcttca tcggcgctgt caaggacacc tcgctggtgg
       61 cgatcgtgaa cctctacgac ctcaccggca ccctgcgcct ggcgatgggc gactcaagct
      121 ggcgcccctt cttcgtcgag atgtacctgg tggtgagtct gatctacctc gcgatgggct
      181 tttcgatcgc gaagtacggt cgctatctgg agcggcgcta tgcgctgatc accaaatgaa
      241 ctctgaggcc ttcctcacca tcaacggcgt tcacaaatgg ttcggcgcct tccatgcgct
      301 ggacgactgc tcgctcgacg ttgcgcgcgg cgaaaagatc gtcgtttgcg gcccttccgg
      361 ctcgggcaag tcgacgctga tccgctgcat caaccgcatc gagcaccacg acgccggccg
      421 catcgccttc aacggtaccg agctggacga cggccgcccc gccgtgcagg cgcgcaagga
      481 aatcggcatg gtgtttcagc agttcaacct gttcccgcac ctcacggtgt tggacaacgt
      541 gatgctggcg cccacgctag tgaagaggca ggacgcgcac tcggcacgag aagaggcgct
      601 ggccttgctc aaacgcgtgc gcatcgtcga acaggccggc aagttcccgg gccagttgtc
      661 tggtggccag cagcagcgcg tggccattgc gcgtgccctg gcgatgcagc ctcagctgat
      721 gctcttcgat gagcccactt cggcgctcga tccggagatg gtcggcgagg tgctggatgt
      781 gatgcgcgag ctggcgcaag acggcatgac catgatcgtg gtgactcacg agatgggctt
      841 tgcgcgccag gtggcggatc gcgtcgtctt catggaggcg ggccgcatcc tcgaaatcca
      901 gcctccggaa ctcttcttcg atcggccgca gagcgagcgt acgcgcgctt tcctggggaa
      961 gatcttgtcg cattgattgg accatccatg aaacgtcctt atgtacttgc cttgcacggc
     1021 ggggcaggca ccattgccgc cggctccgct gccgacgagc agccctatcg cgaggccttg
     1081 ctgttctctc tggcggccgg tgaagccgtg ctggccgccg gcggctcagc cctggatgcg
     1141 gtggttgcga ccgtcgtcgc gctggagaac tgcccgctgt tcaacgccgg ccacggcgcg
     1201 gtattcaccg ccgatgcctt gcacgaactg gatgccagcg tgatggacgg cagcagcctg
     1261 ggcgccggtg ccgtggcggg cgcaaggcag atacgcaatc ccgtcatagc ggcgcgcgag
     1321 gtgctgcgcc agggccgcag cgtgctgctc ggtggcgaag gcgccgatcg cttcgcgcgc
     1381 gagcggggcc tggagatggt cgaccccagc tacttctcga cgccgcatcg cctcgagcag
     1441 ctgcgcgcgg tccaggcccg cgcggcggac ctgcagacgc tggaccacga cagccaggcc
     1501 atggcgaccg aaggcgaggg ccggtatggc acggtgggtg ccgtggccct ggaccgagac
     1561 ggacatctgg cggccgccac gtccaccggc ggcatgacca acaagcagcc agggcgcatc
     1621 ggcgacacgc cggtggtcgg cgccggggtc tatgcgaacg atgccacctg tgcggtgtcc
     1681 ggcaccggaa ccggcgagca cttcctgcgt gcttgtgcag ggcatgacgt gcatgcccgg
     1741 atggcctacc tggggcaaag cctggatcag gccgtcgagg cgaccctgca ccagggcatc
     1801 gaaccgctgg gcggcaaggg cgggctgatc gccatcgatc gccgcggcca gttgtcgctg
     1861 cgtttcaact ctacgggcat gtaccgcgcc tgggtacgcg agggcgccgt ccggcgtgcc
     1921 gccgtcttcg ctgactgaag cctcgcatgc gcatttcgga aatcattctg aggcgggtcg
     1981 acctgccttt tcgcgcgccg ttcaagacct cacgctg
//
LOCUS       DASUCY010000259         5026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_921135, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000259 DASUCY010000000
VERSION     DASUCY010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 5026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5026
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921135"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="VFV25_04935"
     CDS             complement(<1..998)
                     /locus_tag="VFV25_04935"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEY1392694.1"
                     /translation="MTKKLLALFLLAFSLAGARAQAPQPPEIAAKSWLLLDMSTNQVL
                     ASKDADAPAEPASLTKVMSAYLVFQALRDKKLDLNQELTVSERAWRTGMTDASRMYIQ
                     VGTKVKVDDLVKGMIVQSGNDATVQLAEAVSGSVEQFIELMNKQAQAFGLKATVFKNP
                     DGLPAPGHVSTARELAIISARLLQDFPETVRYDSMKDFTYNKIKQGNRNLLLYRDPTV
                     DGLKTGYTDAAGYCMIATAKRDFPNGQRRLLSVVLGAASKEARANESQKLLNWGYSAF
                     DAVKLFDANQAVVTPDVWKGSVKQAKLGSAQAVYVNVPHGEGGKLKTEIQRTDPLVA"
     gene            complement(1046..1672)
                     /locus_tag="VFV25_04940"
     CDS             complement(1046..1672)
                     /locus_tag="VFV25_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY1392695.1"
                     /translation="MNIQTKRTSIVGPAGTLECAVDAPLDTAPRAVMVICHPHPQHGG
                     TMDNKVVQTLARAALQLGWRAVRFNFRGVGGSAGAWDEGRGEVDDTLTVIEAQRDPAL
                     PLVLAGFSFGGAMAAIAAARLPEGRRAQRLLLVAPSSRNFAMPAVPEDTVLIHGEADD
                     VVPLSASLDFARPLSLPVIVVPGVGHFFHGQLPLLKRLVVRELGQALH"
     gene            complement(1679..2005)
                     /locus_tag="VFV25_04945"
     CDS             complement(1679..2005)
                     /locus_tag="VFV25_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEY1392696.1"
                     /translation="MSYYERHIFFCLNQRDNGEACCADHDAQGCFEHCKKRVKAEGLA
                     GPGKIRVNKSGCLDRCAGGPVAVVYPEAVWYTYVDRDDIDEIIESHLKQGKVVERLLL
                     PPDVGR"
     gene            complement(2072..3211)
                     /locus_tag="VFV25_04950"
     CDS             complement(2072..3211)
                     /locus_tag="VFV25_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEY1392697.1"
                     /translation="MAALTLPRTHRSSAWPLYWAVFILIVYASLHPFVWSWPAHANWG
                     MLILPYSPYWTRFDVVSNLCGYMPLGALLCGALLRDGVPPRRAFLVAVLAGSALSWSM
                     ETLQYLVPSRVPSITDWALNSAGNFAGAALALAIQGLGWVDRWQAMRAHWFTTRSPAA
                     LSLLVLWPVGLLFPPSLPLAQGQVLPRINSVLAELLLGTPLEGWIEAPADQLWVPLSP
                     GGELLTIALGLLAPVWLAYSFTQPGWRRIVLLLGALLLGLATTTLSTALNFGPDHALA
                     WITAPVRPALALALAIGVLLAWLPGRGAAGAGLVAITLMLTLVNQAPADAYFTESLQA
                     WEQGRFIRFHGIAQWLGWAWPYAALVYLLISVTKQSDEGQQGPAR"
     gene            complement(3220..3651)
                     /locus_tag="VFV25_04955"
     CDS             complement(3220..3651)
                     /locus_tag="VFV25_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEY1392698.1"
                     /translation="MLWIKAFHIVFVASWFAGLFYLPRFFVNLAQVPADSHAERERLL
                     LMAYRLYRFSSLLMVPALLLGGILWLGYGIGRGPGNHWLHLKLLLVLGAIGYHHYCRR
                     LLREFEQLSPRRRSERWYRVFNELTVLLFTLIVVLAVVKPF"
     gene            3982..4971
                     /gene="hemB"
                     /locus_tag="VFV25_04960"
     CDS             3982..4971
                     /gene="hemB"
                     /locus_tag="VFV25_04960"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEY1392699.1"
                     /translation="MAFPQQRPRRLRRDAFTRALVRENNLTPSDFILPVFVLDGKDQA
                     QDVPSMPGVQRLSLDRLYGVAEQCLKLGIPVLALFPVIDPALKTPDGREALNPEGLVP
                     RVVRGLKQNFPELGILTDVALDPFTSHGQDGLLDERGYILNDETVKVLTQQALVQAEA
                     GVDIVAPSDMMDGRIGAIRQALEAKGRIHTRIMAYSAKYASAFYGPFRDAVGSKSSLG
                     KADKKTYQMDPGNSDEALREVALDIAEGADMVMVKPGMPYLDIVRRVKDEFRVPTFAY
                     QVSGEYAMLKAAAANGWLDHDAAMMEALLAFKRAGADGVLSYFALEAARLLKA"
BASE COUNT          898 a         1669 c         1695 g          764 t
ORIGIN      
        1 gccaccagcg ggtcggtgcg ctggatctcg gtcttgagct tgccgccttc gccatgcggc
       61 acgttgacat agacggcctg ggcgctgcct agcttggcct gcttgacgct gcccttccag
      121 acatccggcg ttacgacggc ctggttggcg tcgaagagct tgacggcgtc gaaggcgcta
      181 tagccccagt tcagcagctt ctggctctcg ttggcgcgcg cctccttgga ggcggcgccc
      241 agcaccacgc tgagcaggcg gcgctgcccg ttggggaagt cgcgcttggc ggtggcgatc
      301 atgcagtagc cggccgcatc ggtgtagccg gtcttgaggc cgtccaccgt cgggtcgcga
      361 tacagcagca ggttgcggtt gccctgcttg atcttgttgt aggtgaagtc cttcatgctg
      421 tcgtacctca ccgtctcggg gaagtcctgc agcagccgcg cggagatgat ggccagttcg
      481 cgcgcggtgc tgacatggcc gggcgcgggc aggccgtcgg ggttcttgaa caccgtggcc
      541 ttgaggccga aggcctgggc ctgcttgttc atcagctcga tgaactgctc gacggagccg
      601 ctcaccgcct cggcgagctg cacggtggcg tcgttgccgc tctgcacgat catgcccttg
      661 acgaggtcgt cgaccttcac cttggtgccg acctggatgt acatgcgcga ggcatcggtc
      721 atgccggtgc gccaggcgcg ctcggagacg gtgagctcct ggttcaaatc cagcttcttg
      781 tcgcgcagcg cctggaacac gaggtaggcg ctcatcacct tggtcaggga ggccggctcg
      841 gcaggcgcgt cggcgtcctt cgaagcgagc acctggttgg tgctcatgtc cagcagcagc
      901 caggacttgg cggcgatctc gggcggctgc ggggcctggg cgcgcgcgcc ggcgaggctg
      961 aaggcgagga ggaagagagc gaggagtttc ttggtcatca ttcggttctg gctggaggct
     1021 gttagtggtc gcttgtgtgc attcgtcaat gcagggcttg acccagctcg cgcaccacca
     1081 ggcgcttgag cagcggcagc tgcccgtgga agaaatgccc cacgcccggc accacgatga
     1141 cgggcaggct caaggggcgc gcgaagtcga gcgaggccga aagcggcacc acgtcgtcgg
     1201 cttcgccatg gatcaatacg gtatcttcag gcacggccgg catcgcgaag ttccgcgacg
     1261 aaggtgcaac cagaagcaaa cgctgcgcac gccggccttc gggcagccgc gccgccgcga
     1321 tcgccgccat agcgccgccg aaggaaaagc cggccagcac cagcggcagc gccgggtcgc
     1381 gctgtgcttc gatcacggtc agcgtgtcgt cgacctcgcc gcggccttcg tcccaggcgc
     1441 ctgccgagcc gcccacgccg cggaaattga agcgcaccgc gcgccagccg agctgcagcg
     1501 cggcccgcgc cagggtctgc accaccttgt tgtccatcgt gccgccgtgc tgaggatgcg
     1561 ggtggcagat caccatcaca gcgcgcgggg ccgtgtccag cggtgcatcc accgcgcatt
     1621 ccaaggtgcc ggcggggccg acgatgctgg tgcgcttggt ctggatgttc atcgggcttc
     1681 agcggcccac atcgggtggc agcagcaggc gctcgacgac cttgccttgc ttcagatggg
     1741 attcgatgat ctcgtcgatg tcgtcgcggt cgacataggt gtaccagacg gcctcgggat
     1801 agaccaccgc caccggaccg ccggcgcagc ggtcgaggca gccggatttg ttgacgcgga
     1861 tcttgcccgg cccggccagg ccctcggcct tgacgcgctt cttgcaatgc tcgaagcagc
     1921 cctgggcgtc gtgatcggcg cagcaggcct cgccgttgtc gcgctggttc aggcagaaga
     1981 agatgtgccg ctcgtaatag ctcatgggtg aattctaggt gttcacccta ggtcctgtta
     2041 acgctacggg gaggctcgcg ggccgcatcc gctaccgcgc cggcccctgc tgcccctcgt
     2101 cgctttgctt cgtcacgctg atcagcagat agaccagcgc cgcgtagggc caggcccaac
     2161 ccagccattg cgcgatgccg tggaagcgga tgaagcggcc ctgctcccag gcctgcaggc
     2221 tctcggtgaa ataggcgtcg gccggcgcct ggttgaccag ggtcagcatc agcgtgatgg
     2281 ccaccagccc ggcgccggcc gcgccgcggc ccggcagcca ggccagcagc acgccgatcg
     2341 ccagcgccag cgccagcgcc gggcgcaccg gcgcggtaat ccaggccagg gcgtggtcgg
     2401 ggccgaagtt gagtgcggtc gacaaggtgg tggtggccag ccccagcagc agtgcaccga
     2461 gcagcagcac gatgcgccgc cagccgggct gcgtgaagct ataggccagc catactgggg
     2521 ccaggagccc caaggcaatg gtcagcagct cgccgccggg cgacagcggc acccacagct
     2581 ggtcggccgg cgcctcgatc cagccttcca gcggcgtgcc cagcagcagc tcggccagca
     2641 cgctgttgat gcgcggcagc acctgaccct gggccagggg cagcgaaggc gggaacagca
     2701 gccccaccgg ccaaagcacc agcaggctca aggccgccgg gctgcgcgtc gtgaaccagt
     2761 gcgcacgcat ggcctgccag cgatcgaccc agcccaggcc ttggatcgcc agggccagcg
     2821 ccgcgccggc gaagttgccg gccgagttca gcgcccagtc ggtgatcgag ggcacgcgcg
     2881 agggcaccag gtattgcagc gtctccatgc tccacgacaa agccgagccg gcgagcaccg
     2941 caaccaggaa ggcgcggcgc ggcggcacgc cgtcgcgcag cagggccccg cacaacaggg
     3001 cgcccagcgg catgtagccg cacagattgg agacgacgtc aaagcgcgtc cagtagggcg
     3061 aatagggcag gatcagcatg ccccagttcg catgggccgg ccagctccac acgaagggat
     3121 gcaggctggc gtagacgatc aggatgaaca cggcccagta caggggccag gcggagctgc
     3181 ggtgcgtgcg gggaagggta agtgctgcca cgccaggctt taaaagggtt tgaccaccgc
     3241 cagcacgacg atgagcgtga acagcagcac cgtcagctcg ttgaagacgc gataccagcg
     3301 ctcgctgcgg cggcgcggcg acagctgctc gaactcgcgc agcagcctgc ggcagtaatg
     3361 gtgatagccg atcgcgccca gcaccagcag cagcttcaag tgcagccaat ggttgcccgg
     3421 cccgcggcct atgccatagc ccagccaaag gatgccgccg agcaacagcg ccggcaccat
     3481 cagcaggctg ctgaagcgat agagccgata cgccatcagc agcagccgct cacgctcggc
     3541 atggctgtcg gccggcacct gggccaagtt gacgaagaag cgcggcagat agaacagacc
     3601 cgcgaaccaa ctcgcgacga agacgatgtg gaaggctttg atccagagca tcggcccatt
     3661 atcgcgaggg tggccgagag agcctggggt tgcgccggta ctgccggaaa atcccgccgc
     3721 cgcaaaaaaa gaagccccgg cataaccggg gctgcaaagc cttatcgctg tcatcacggt
     3781 aaggctcctg ctcagggagg aaaagcaggg aacgacacgc cttgcgacgt atcattcacg
     3841 ggccagttta gcagccgaca gcttgcaaat gtctgtcgga tgacagcctg gacaatgtaa
     3901 aacgccccgc gaatcggggg aggcggctcc cgcccagtcc actgctgtac agacatccat
     3961 caacccgcaa gaaaaagcga catggccttt cctcaacaac gtccgcggcg gctgcgccgc
     4021 gacgccttta cccgtgccct ggtgcgcgaa aacaatctca cgccttccga cttcatcctg
     4081 ccggtcttcg tgctcgacgg caaagaccag gcgcaggacg tgccctccat gcccggcgtg
     4141 cagcgcctga gcctggaccg gctctacggc gtggccgagc aatgcctgaa gctgggcatc
     4201 ccggtgctgg cgctcttccc ggtgatcgat ccggcgctca agacccccga cggccgcgag
     4261 gcgctcaacc ccgagggttt ggtgccacgc gtcgtgcgcg gcttgaagca gaactttccc
     4321 gagctgggca tcctgaccga cgtcgcgctc gaccccttca ccagccatgg ccaggacggc
     4381 ctgctggacg agcgtggcta catcctcaac gacgagacgg taaaggtgct gacccagcag
     4441 gcgctggtgc aggccgaggc cggtgtcgac atcgtcgcgc cctccgacat gatggacggc
     4501 cggatcggag cgattcgaca agcgctcgag gccaagggcc ggatccacac ccgcatcatg
     4561 gcctacagcg cgaagtacgc ctcggccttc tacggcccct tccgtgacgc ggtgggctcc
     4621 aagtccagcc tgggcaaggc cgacaagaag acctaccaga tggaccccgg caacagcgac
     4681 gaggctttgc gcgaagtcgc gctggatatc gccgaggggg ccgacatggt gatggtcaag
     4741 cccggcatgc cttacctcga catcgtgcgc cgcgtgaagg acgagttccg cgtgccgacc
     4801 ttcgcctacc aggtgagcgg cgaatacgcg atgctcaagg ccgccgccgc caacggctgg
     4861 ctggaccacg acgcagcgat gatggaagcc ctgctggcct tcaagcgcgc cggcgccgac
     4921 ggcgtgctga gctatttcgc gctcgaggcc gcgcggctgc tcaaggcctg aggcccgaaa
     4981 ctgacaccgc ggctggcgac cctcaagcca atgcgaacgg accgcc
//
LOCUS       DASUCY010000260         5099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_889014, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000260 DASUCY010000000
VERSION     DASUCY010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 5099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5099
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_889014"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..1032
                     /locus_tag="VFV25_04965"
     CDS             73..1032
                     /locus_tag="VFV25_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY1392700.1"
                     /translation="MSTSAGTDAVIEARGLIKRFGDKTAVDKLDMTVRRGEIFGFLGP
                     NGSGKTTSIRMLCGLLTPDGGTGRTLGHDIVKEAGLIKRQVGYMTQRFGLYDDLTIRE
                     NLDFIGRMYGMQARRERVVQAIERLGLGGRRDQLAGTLSGGWKQRVALAACMLHEPQL
                     LLLDEPTAGVDPQARRAFWEEIHELAAGGITVLVSTHYMDEAERCHRLAYIAYGRLLA
                     SGTPDELVGAAGLKTWEGKGERIAALAAQLEAQPGVELVTRFGNSLHVSGRDAAALDA
                     AIAGFKEACQWQRLPTTLEDIFIFLMRQAEDQYQAQGRGPVGT"
     gene            1142..2275
                     /locus_tag="VFV25_04970"
     CDS             1142..2275
                     /locus_tag="VFV25_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY1392701.1"
                     /translation="MTSFSFARLAAMMLKEFIQLRRDRLTFAMMIGVPLLQLTLFGFA
                     INSDPKSLPTIVVAQEQGVFTRSIVRAMENTGYFRVVQVTGDPGAADSALAGGEAQFA
                     LTVPVGFARALQRGERPQLLVEADATDPAATGNAIAALQQLAGSALQHDLVGALARPP
                     GAGAPFDLVIQRRYNPEGVTQYNVVPGLMGVILTMTMIMMTALGITREVERGTMENLL
                     ATPVQAAEVMVGKIAPYIVIGYVQVAVVLLAARWLFSVPFVGNLGLLLLGTLVFIAAN
                     LTVGITVSSVARNQTQAMQMTFFFFLPSILLSGFMFPFRGMPGWAQAIGEVLPLTHFL
                     RIVRGVMLKGNSVADLWPQIWPLLIFIAVMMGVGLQRFRRTLD"
     gene            2298..3515
                     /locus_tag="VFV25_04975"
     CDS             2298..3515
                     /locus_tag="VFV25_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019699892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HEY1392702.1"
                     /translation="MDIVRTIRDFNQGRDPERLLLKFRNMRRSPFVFLRGTCHLFHAK
                     LPNEKVMSKAPPSWVCGDLHLENFGSYKGDNRLVYFDLNDFDEAALAPVTWELLRVLT
                     SILVGAETLRVTQAESLSLCEAFLDAYCAALSKGKARWVERETAHGLVGELLDRLQGR
                     LRPQFLDSRTDRKGRRRKIRIDGKRALAVTDEQRHRVVTRVDEFAAMQEHPAFYEVLD
                     VARRIAGNGSLGVDRYAILVRGKGSPDGNYLLDLKQALPSSLVPQLRARLPKLKQPDW
                     KSEAQRVVTLQRRMQAVSMAFLQPLRMGRQSYVLRALQPTEDRVVLDGAAQSLAQLKG
                     VMQVMGECAAWAQLRSAGREGSAIADEWVAFGERSKKWRRELMALARDCAAGVEADWK
                     NYSAAYDEGAFKI"
     gene            3670..4182
                     /locus_tag="VFV25_04980"
     CDS             3670..4182
                     /locus_tag="VFV25_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392703.1"
                     /translation="MPHPSLSPWRGDAAPTLFDRLLASLGGGARAAAKSAGKRTTTQP
                     GGESRLGLSSISSAPLHEALGTLLREVKLMPGTYVAKFVPITSASGRTGYAVFIRLDE
                     YVKHLMDRTKEIEGRVVRQMRKRFEIEVTQIYWRYSSRARTPWDAAAREGKAGKPRSR
                     SVGSAQASSR"
     gene            4217..4690
                     /locus_tag="VFV25_04985"
     CDS             4217..4690
                     /locus_tag="VFV25_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392704.1"
                     /translation="MITKLERRIASFILLFWAAALPALADSMSLSSISDSIATSVGSI
                     SNSIKNSSNSSSKTDRTAEGDYKVIEVAAVAERPGAVRMKLQALAEGGAGKDEFYLYL
                     PQAAYEQAKLAPGRTVTARQRPYGVEFAGGEPRQAFFLALDDDWYRELQAKAVTL"
     gene            4822..>5099
                     /locus_tag="VFV25_04990"
     CDS             4822..>5099
                     /locus_tag="VFV25_04990"
                     /inference="COORDINATES: protein motif:HMM:NF021437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2145 domain-containing protein"
                     /protein_id="HEY1392705.1"
                     /translation="MSAEQQDVLLRFGAIVKDELEKSGQSLVLITRSGLDLSRFGVRY
                     SHAGLSLKASRNAPWSVRQLYYACDERKPRLYDQGMSGFLLGADDPSTG"
BASE COUNT          845 a         1683 c         1713 g          858 t
ORIGIN      
        1 gccggccgct gccgatgcct tcaagctgcg gcctggccag ccggtggaag tgacgctggg
       61 gcgcaaggcg tcatgagcac ctccgcaggg accgatgccg tcatcgaagc gcgcgggctg
      121 atcaagcgct tcggcgacaa gaccgcggtc gacaagctgg acatgacggt gcggcgcggc
      181 gaaatcttcg gcttcctcgg gcctaacggc agcggcaaga cgacctcgat ccgcatgctg
      241 tgcggcctgc tcacgcccga cggcggcacc gggcgtacgc tgggccacga catcgtcaag
      301 gaggccgggc tcatcaagcg ccaagtgggc tacatgacgc agcgcttcgg cctctacgac
      361 gacctcacga tacgcgagaa cctcgacttc atcggccgca tgtacggcat gcaggccagg
      421 cgcgagcgcg tcgtccaggc catcgagcgc ctgggcctgg gtggccggcg cgaccagctg
      481 gccgggacgc tctcgggcgg ctggaaacag cgcgtggcgc tggccgcctg catgctgcac
      541 gagccgcagc tgctgctact ggacgaaccc acggccggcg tggacccgca ggccaggcgt
      601 gccttctggg aggagatcca cgagcttgcg gccggcggca tcacggtact cgtgtccacc
      661 cattacatgg acgaagccga gcgctgccat cgcctggcct atatcgccta tgggcgcctg
      721 ttggccagcg gcacgcccga cgagctggtg ggcgctgccg gcctcaagac ctgggagggc
      781 aagggcgagc gcatcgccgc gctggcggcg caactcgagg cgcagccggg cgtggagctg
      841 gtcacgcgct tcggcaattc gctgcacgtg agcgggcgcg acgcagctgc actcgacgcc
      901 gcgatcgcag gcttcaagga agcctgccaa tggcagcgcc tgccgaccac gctggaagac
      961 atcttcatct ttctgatgcg ccaggccgaa gaccagtacc aggcgcaggg cagggggccc
     1021 gttgggacgt gaggtatcca gtgccaacct gtctttcccg agccgagcgc cgggccgctc
     1081 ccaagccggc ccgcgtcccc cccggggacc ggccgacgta ctcgtcgggc gaggggtggt
     1141 catgacctcg ttcagcttcg cccgcctcgc cgcgatgatg ctcaaggagt tcatccagct
     1201 gcggcgcgac aggctcacct tcgcgatgat gatcggcgtg ccgctgctgc agctgacgct
     1261 gttcggcttc gcgatcaata gcgaccccaa gagcctgccc accatcgtgg tggctcagga
     1321 gcagggggtg ttcacgcgca gcatcgtgcg agcgatggag aacaccggct atttccgcgt
     1381 ggtgcaggtc acgggcgacc ccggcgctgc cgactcggcg ctggcgggcg gcgaagccca
     1441 gttcgcgctg accgtgccgg tgggctttgc gcgcgcactg cagcgcggcg agcggccgca
     1501 gctgttggtg gaggcagatg ccaccgatcc cgccgccacc ggcaatgcga tcgccgcgct
     1561 tcagcagctg gccggctccg ccttgcagca cgacctggtg ggcgcgctgg ctcgcccgcc
     1621 gggtgccggc gcgcctttcg acctcgtgat ccagcgccgc tacaaccccg agggcgtcac
     1681 gcaatacaac gtcgtgcccg gcctgatggg cgtgatcctc acgatgacga tgatcatgat
     1741 gaccgcgctg ggcatcacgc gcgaggtcga gcgcggcacg atggagaacc tgctcgccac
     1801 gccggtgcag gcagccgagg tgatggtggg caagatcgcg ccctacatcg tgatcggcta
     1861 tgtgcaggtg gcggtggtgc tgctcgccgc gcgctggctc ttcagcgtgc ccttcgtcgg
     1921 caacctgggc ctgctgctgc tgggcaccct ggtcttcatc gcggccaatc tgacggtggg
     1981 catcaccgtg tccagcgtgg cgcgcaacca gacccaggcg atgcagatga ccttcttctt
     2041 cttcctgccg tccatcctgc tgtcgggctt catgttcccg ttccgcggca tgccgggctg
     2101 ggcgcaggcc atcggcgaag tgctgccgct cacgcatttc ctgcgcatcg tgcgcggcgt
     2161 gatgctcaag ggcaacagcg tggccgatct atggccgcag atctggcctt tgctgatctt
     2221 catcgccgtg atgatgggcg tgggcctcca gcgcttccgg cgtacgctgg attgaatacc
     2281 aagcacaaag gcagacgatg gacatagttc ggacgatccg ggacttcaac caaggccgcg
     2341 accccgagcg gctgctgctc aagtttcgca acatgcgccg cagccccttc gtgttcctgc
     2401 gcggaacctg ccacttgttc catgccaagc tgccgaacga gaaggtcatg agcaaggcgc
     2461 cgccgagctg ggtgtgcggc gacctgcacc tggagaactt cggcagctac aagggcgaca
     2521 accggctcgt ctacttcgat ttgaacgact ttgacgaagc cgcgctggcc ccagtcacgt
     2581 gggagctgct gcgcgtgctg accagcatcc tggtcggcgc cgagaccctg cgcgtcaccc
     2641 aggcggaatc gctcagcctg tgcgaggcct tcctcgacgc gtattgcgcg gcactgagca
     2701 agggcaaggc gcgctgggtc gagcgcgaaa cggcgcatgg cctggtgggc gaattgttgg
     2761 atcgcctgca ggggcggctg cgcccgcagt tcctcgacag tcgcaccgac cgcaagggtc
     2821 gccggcgcaa gatacgcata gacggcaagc gggcattggc ggtgaccgac gagcagcggc
     2881 atcgcgtcgt tacgcgggtc gacgaattcg ccgccatgca ggagcatcca gccttttacg
     2941 aggtgctcga tgtggcgcgc cgcatcgctg gcaacggcag cctgggcgtc gaccgctatg
     3001 ccatcctggt gcgcggcaag ggctcgccgg acggcaacta cctgctggac ctcaagcagg
     3061 ccttgccctc gtcgttagtg ccccagttgc gggccaggtt gcccaagctg aaacagcccg
     3121 attggaaatc cgaggcgcaa cgcgtggtca cgctgcagcg ccgcatgcag gcggtatcca
     3181 tggccttcct gcagccgctg cgcatgggcc gtcagtccta tgtgctgcgc gccctgcaac
     3241 ccaccgagga ccgcgtcgtg ctcgacggcg cggcccagag tctggcccag ctcaagggcg
     3301 tgatgcaggt gatgggcgaa tgcgcggcct gggcccagct gcgcagcgca ggccgcgagg
     3361 gctcggccat cgccgacgaa tgggtggcct tcggcgagcg cagcaagaaa tggcggcgcg
     3421 agctgatggc attggcgagg gattgcgcgg ccggggtaga agcggattgg aagaactatt
     3481 ccgccgccta cgatgagggt gccttcaaga tctgatgccc gcttgtcaag ctgcgtcgcg
     3541 ccggttgtgg tttccaagag tttcagaggc tgacaagcaa caatttggta cgaaatccgg
     3601 tgatttcgca cttgccggaa ttgacgcccg gcaaacaatt ggagggcttc aaacgacaag
     3661 gagggccgcg tgccccatcc cagcctttca ccctggcgcg gcgacgccgc tccaacactg
     3721 ttcgatcgcc tgctggcctc gcttggcggt ggcgcccgtg ccgcagctaa gtccgctggt
     3781 aagcggacaa cgacgcaacc cggcggggag tcgagactgg gtctcagcag catttcctca
     3841 gctcccttgc acgaggctct gggcacgctg ctgcgcgaag tcaagctgat gccgggcacc
     3901 tatgtcgcca agtttgtgcc gatcaccagc gccagcggcc gcaccgggta tgccgtgttc
     3961 atccggctgg acgaatatgt caagcatttg atggacagga ccaaggagat cgaggggcgc
     4021 gtggtgcggc agatgcgtaa gcgcttcgag atcgaggtga cgcagatcta ctggcgttac
     4081 agctcgcgcg ccaggacgcc ctgggacgcc gccgcgcgcg aaggcaaggc cggcaaaccc
     4141 aggtcgcgat ccgtcgggag cgcgcaagct tcgtcgcgct aacatccgcg catcaggact
     4201 gctgcgtgga gtcggaatga tcaccaagct cgaaaggcgt atcgcctcgt tcatcctgct
     4261 gttctgggcc gctgcgctgc ctgcgctggc cgacagcatg tccctgtctt ccatctcgga
     4321 cagcatcgcc acctcggtgg gcagcatctc gaattccatc aagaactcga gcaacagctc
     4381 atccaagacc gatcgcacgg ccgaaggcga ctacaaggtc atcgaggttg cagccgttgc
     4441 cgagcggccc ggcgccgtgc gcatgaagct gcaggcgctt gccgaaggcg gcgccggcaa
     4501 ggacgagttc tacctctacc tgccccaggc tgcctacgag caggccaagc tggcgcccgg
     4561 gcgcaccgtg acggcgcgcc agcgccccta tggcgttgaa tttgccggtg gcgagcctcg
     4621 gcaggccttc ttcctcgcac tcgacgacga ctggtatcgg gagctgcaag ccaaggctgt
     4681 aacgctctga gcgtgcgccc ctggggtctc gggcgaggcc ttcgctcggc gagcgcctcg
     4741 gcgctgctcc tgttgtgcgg cctgctgtgg agtccttccg gctgggccga ctctctgcgc
     4801 ttttgcgacc ggcagacgcg aatgagtgcc gagcagcagg acgttctgct gcgcttcggt
     4861 gccatcgtca aggatgagct cgaaaaatcc ggccagagcc tggtgctcat tacccgctcg
     4921 ggcctggacc tgagccgctt cggcgtgcgc tattcgcacg ctgggctcag cctcaaagcc
     4981 agccggaatg cgccctggtc ggtgcgccag ctctattacg cctgcgacga gcgcaagcct
     5041 cgcctctacg accagggcat gtcaggtttc ctcctcggtg ccgacgatcc ttccaccgg
//
LOCUS       DASUCY010000261         3237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_374950, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000261 DASUCY010000000
VERSION     DASUCY010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374950"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..920
                     /locus_tag="VFV25_04995"
     CDS             3..920
                     /locus_tag="VFV25_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEY1392706.1"
                     /translation="MRKSLELQGELGPVFDVTLDNEDGAPVGAEREQAHLIAELATSP
                     LNRYGRVGARLLPVHHAAFADVARTLVERAGDSLAYLMIPKPRDHADFAAAADEIDRL
                     SRAAGLQRPIPLHALIETHGALREVDALAAHPRIESLSFGLMDFVSAHRGAIPQSAMS
                     AAGQFEHPLVLRAKLEIAAACHGHAKTPSHCVVTEFKDQRALQAAAEKASRQLGYTRM
                     WSIHPAQIRPIVDAFSPTVAEIDQAIEIITAAQAAQWAPIQHHGTLHDRASYRYFWNV
                     LERAHRTSFSDGPQLPAEVREAFFSDTAP"
     gene            956..1549
                     /locus_tag="VFV25_05000"
     CDS             956..1549
                     /locus_tag="VFV25_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY1392707.1"
                     /translation="MLVLKTYPHPLELRTPRTLLRQWRDDDLPAWIAMNADAEVRRHF
                     PSVHTAEEARGEAQRIHDALQQRGWGFWALEIPGQLKFAGCVGLLVPTYEAHFLPAVE
                     IGWRLSREAWGHGYAREAASAAAEFAFKELDLDEIVAITIPDNTASRRVMERIGMRHD
                     AQGDFEHPRIAAGHPKRRHVLYRLDRASFRRIAAQRS"
     gene            1914..2435
                     /locus_tag="VFV25_05005"
     CDS             1914..2435
                     /locus_tag="VFV25_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392708.1"
                     /translation="MKRVLTLSCVAAALAFSGIAMAQTSDSSSQQQAAPAKHKAAKKS
                     QSRKEAQPKANNQVTEPISDGQLAVANRVLTGKAQCEFGQTVEISPLESKKGAFVVSY
                     KKASYVMVPEETTTGAVRLEDKKNGIVWLQIANKSMMMNQKLGQRMVDDCMLGEQRAA
                     VDAMKAAAQSANK"
     gene            2459..>3237
                     /gene="prpD"
                     /locus_tag="VFV25_05010"
     CDS             2459..>3237
                     /gene="prpD"
                     /locus_tag="VFV25_05010"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HEY1392709.1"
                     /translation="MPAPASKVRPKPDKVLVDIVDYVTKYRVDSRLAYETARNCLIDT
                     LGCGLEALEYPACTKLLGPTVPGSFVPNGAKVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRSALAAGKQPLLMRDVLTAMIKAHEIQGCIAL
                     ENSFNKVGLDHVVLVKVASTAVVSQLLGLSREEILNAVSLAWVDGQALRTYRHAPNTG
                     SRKSWAAGDATSRAVRLALIARTGEMGYPSVLSAKGWGFYDV"
BASE COUNT          565 a         1159 c         1043 g          470 t
ORIGIN      
        1 gcatgcgcaa gagcctggag ctgcagggcg agttggggcc ggtgttcgac gtcacgctgg
       61 acaacgagga cggcgcgccg gtgggcgccg agcgcgagca ggcccacctg atcgccgagc
      121 tggcgacttc gccgctcaat cgatatggcc gcgtcggcgc gcgcctgctg ccggtgcacc
      181 acgcggcctt cgccgatgtc gcccgcacgc tggtcgaacg cgcgggcgat agtctggcct
      241 atctgatgat tcccaagccg cgcgaccacg ccgacttcgc ggctgccgcc gacgagattg
      301 accgcctctc gcgcgccgcc gggctgcaaa ggcccatacc gctgcatgcg ctcatcgaaa
      361 cccacggcgc cctgcgcgaa gtggacgcac tcgccgcgca cccgcgcatc gaatcgctgt
      421 ctttcgggct gatggatttc gtctccgcgc atcgcggcgc gatcccgcaa tcggccatga
      481 gcgcggccgg gcaattcgag catccgctgg tgctgcgcgc gaagctggag atcgcggcgg
      541 cctgccacgg ccatgcgaag acgccctcgc attgcgtcgt gaccgagttc aaggaccagc
      601 gcgcgctgca ggcagccgcg gagaaggcca gccgccagct cggctacaca cgcatgtgga
      661 gcatccaccc ggcccagatc cggcctatcg tcgatgcctt ctcgcccacc gtcgccgaga
      721 tcgaccaggc catcgagatc atcacggccg cgcaggccgc gcaatgggcg ccgatccagc
      781 accacggcac gctgcacgat cgagcgagct accgctattt ctggaacgtg ctggagcgcg
      841 cgcaccgcac ctccttcagc gatgggccgc agctgcccgc cgaggtgcgc gaagcctttt
      901 tctccgacac cgcgccctga gcggggcggc gccgcatgag gcatcatccg gcgccatgct
      961 cgtcctcaag acctaccccc atcccctgga gctgcgcacc ccgcgcacgc tgctgcgcca
     1021 gtggcgcgac gacgacctgc cggcctggat cgcgatgaac gccgacgcgg aagtacgcag
     1081 gcatttcccg agcgtgcaca cggccgagga ggcgcgcggc gaggcgcaac gcatacacga
     1141 tgccctgcag cagcgcggct ggggcttctg ggcgctggag atcccgggcc agctgaagtt
     1201 cgcgggctgc gtgggcttgc tggtgcccac ctacgaagcc cacttcctgc ccgccgtcga
     1261 gatcggctgg cggctctccc gcgaggcctg gggccatggc tatgccaggg aagcagcgag
     1321 cgcggcggcc gagttcgcct tcaaggaact cgatctcgac gagatcgtcg ccatcacgat
     1381 tcccgacaac accgcctcgc gccgcgtgat ggagcgcatc ggcatgcgcc acgacgcgca
     1441 gggcgacttc gagcacccgc gcatcgccgc cggccatccg aagcgccgcc acgtgctcta
     1501 ccggctcgac cgggcgagct ttcgccgcat cgccgcgcag cgctcttgaa agcctcgttg
     1561 tcgcaggatg ccaacagctt gagagcctga ggcggcgccg aaagggttgg aatcggtaat
     1621 cgccggcgcc gggcgcgtag ctcccgccgg gtcgcttcgc ggcggtcggc acgtcactta
     1681 aacgccataa aacgcgatcc ccctgccgcg gttcgtgatt tctcgcacaa accgtcggac
     1741 cgcgcgcacc atcgagcagc gccgcgacag gggaaggcgc accagtcccg agggccgtgg
     1801 ccgcggattt cctgactgcg cgcttgcgcg ccggatgcaa attgcaacgc ctgtttgtaa
     1861 ccgcacctcc agaattcggg cctcaacctt cccccgtcgt taggagatac cacatgaaac
     1921 gcgtcctcac cctgtcctgc gtcgctgccg ccctcgcctt cagcggcatc gcgatggccc
     1981 agacctcgga cagcagcagc cagcagcagg cggctcccgc caagcacaag gcggccaaga
     2041 agtcgcagtc gcgcaaggaa gcccaaccca aggccaacaa ccaggtcacg gagccgatca
     2101 gcgacggcca gctggcggtc gccaaccgcg tgctgaccgg caaggcccag tgcgaattcg
     2161 gccagacggt cgaaatctcg ccgctggaga gcaagaaagg cgccttcgtc gtttcctaca
     2221 agaaggcctc ctatgtgatg gttcctgagg aaaccaccac cggtgccgtc cgcctggaag
     2281 acaagaagaa cggcattgtc tggctgcaga tcgccaacaa gtcgatgatg atgaatcaga
     2341 agctcggcca gcgcatggtc gacgactgca tgctcggcga gcagcgcgcg gcagtcgacg
     2401 ccatgaaggc cgctgcccag tccgccaaca agtaagccgg ccggcctgga gaggccgcat
     2461 gcctgctccc gcctccaagg tccgccccaa gcccgacaag gtcctggtcg acatcgtcga
     2521 ctacgtgacc aagtacaggg tcgacagccg gctcgcctat gaaacggcgc gcaactgcct
     2581 gatcgacacc ctgggctgcg gactggaggc gctggagtac ccggcctgca ccaagctcct
     2641 gggccctacc gtgccgggca gcttcgttcc gaacggggcg aaggtgcccg gcacccagtt
     2701 ccagctcgat cccgtccagg ccgccttcaa catcggcgcg atgatccgct ggctggactt
     2761 caacgacacc tggctggccg ccgaatgggg ccatccctcg gacaacctgg gcggcatcct
     2821 cgccaccgcc gactggctga gccgcagcgc gttggccgcc ggcaagcagc ccctgctgat
     2881 gcgggacgtg ctcactgcga tgatcaaggc ccacgagatc cagggctgca tcgcgctcga
     2941 gaattccttc aacaaggtcg gcctcgatca cgtggtgctg gtcaaggtcg cgtccaccgc
     3001 cgtggtgtcg cagctgctgg gcctgagccg cgaggagatc ctcaatgcgg tgtcgctggc
     3061 ctgggtcgac ggccaggccc tgcgcaccta tcgccacgcc cccaacacgg gcagccgcaa
     3121 gagctgggcc gcgggcgacg cgaccagccg cgcggtgcgg ctggcgctga tcgccaggac
     3181 cggcgagatg ggttacccca gcgtgctcag cgccaagggc tggggcttct acgacgt
//
LOCUS       DASUCY010000262         2685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1338724, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000262 DASUCY010000000
VERSION     DASUCY010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1338724"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(232..1929)
                     /gene="yidC"
                     /locus_tag="VFV25_05015"
     CDS             complement(232..1929)
                     /gene="yidC"
                     /locus_tag="VFV25_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEY1392710.1"
                     /translation="MTDMRRTLLWVVFTMSLFLLWDGWNKHNGKPSFFSPSPVHQPAS
                     AAGAAPGNGLPAPVAGGQPSAVPGAAASAPVAAQTVTVASDVVKATFDSRGGDLVRLE
                     LLTQKQQDDKNRNIVLFERGNERVYLAQTGLIGNTGALPNHLTVMNLLPGETTLKPGS
                     DALSVVFESPEVGGVRLRKTYTLKRGDYVIGVTHEIINTGTAPVQPQLYLQLVRDGQP
                     PEGQPQSSSFFSGPAAFTGPAMYTQAAHFQKFKFADIVDAADRNKPEHERSASDGWVA
                     MVQHYFASAWMLADGVQRDYQLARLDASVNGVPSYRIAMLTPVGEIAPGASKKIEARL
                     FAGPQEEQKLAALAPGLELVKDYGWFKILAEPLFWLLTQLHKLIGNWGWAIVALVVVL
                     KAAFFWLNASAYRSMAKMKAINPKIQELRERLKDKPQQMQQEMMRIYREEKVNPIGGC
                     LPILIQMPFFLALYWVLLSSVEMRNAPWIGWVTDLSVKDPLYILPAVMTASQLLQTWL
                     NPTPPDPVQAKMMWIMPLVFSVMFFYFPAGLVLYWVTNNLLSIAQQWVINKKLGVAK"
     gene            complement(1926..2240)
                     /gene="yidD"
                     /locus_tag="VFV25_05020"
     CDS             complement(1926..2240)
                     /gene="yidD"
                     /locus_tag="VFV25_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEY1392711.1"
                     /translation="MTPLQLWPRRALMLPIKAYRLLLSPWLGSACRFEPTCSVYALQA
                     VDRHGAAAGSYLAAARLLRCHPWCDGGLDAVPEAPPRLFSRLKAVQVAGLTDPTRTES
                     SS"
     gene            complement(2266..>2685)
                     /locus_tag="VFV25_05025"
     CDS             complement(2266..>2685)
                     /locus_tag="VFV25_05025"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEY1392712.1"
                     /translation="SPHFSLHFSPQHAASAPPATKLSTGVEQMRHPVVDERPASPMPA
                     AWRLGLVVPKRHARRAVTRNLIKRQGRQAFVQALPVLPAGDWLLRLRAPFSSSSGKDA
                     TDSAYKSAASVALKRAVGQELAGLFGSLPAKTASAAR"
BASE COUNT          448 a          858 c          951 g          428 t
ORIGIN      
        1 ctccagagcc agtgcggtga acggtgagga acacgcgccg ggtcgacccc gctactcgga
       61 tgctgccctc ctgcctcgct gcgcgcaacg ccgttcgccc ctgcaagcgc ggggttcgtg
      121 agctaatgcg caccactcga agatggggcg gactttccct ctgcgtcgca gcgcgggccc
      181 caagcaaaaa cgccccggcg gagccgaggc gtttgcatgc agtggtcgcg tctacttggc
      241 cacgcccagc ttcttgttga tcacccattg ctgggcgatc gacagcaggt tgttggtgac
      301 ccagtagagc accaggccgg ccgggaagta gaagaacatc acgctgaaga ccagcggcat
      361 gatccacatc atcttggcct gcaccggatc gggcggcgtc gggttgagcc aggtctgcag
      421 cagctggctg gccgtcatca ccgccggcag gatgtagagc gggtccttga ccgacaggtc
      481 ggtgacccag cctatccacg gcgcattgcg catctcgacg ctggacagca gcacccagta
      541 cagcgccagg aagaagggca tctggatcag gatgggcagg cagccgccga tcgggttgac
      601 cttctcctcg cgatagatgc gcatcatctc ctgctgcatc tgctgcggct tgtccttcag
      661 gcgttcgcgc agctcctgga tcttggggtt gatggccttc atcttggcca tcgagcggta
      721 ggcgctcgca ttgagccaga agaaggcggc cttgagcacc acgaccagcg cgacgatggc
      781 ccagccccag ttgccgatga gcttgtgcag ttgcgtcagc agccagaaca gcggctcggc
      841 gaggatcttg aaccagccgt agtccttgac cagctccagc cccggcgcga gggcggccag
      901 cttctgctct tcctgcgggc cggcaaacaa acgggcctcg atcttcttgg aggcaccggg
      961 cgcgatctcg ccgaccgggg tcagcatcgc gatgcggtag ctcggaacgc cattgaccga
     1021 ggcatcgagg cgggcaagct ggtagtcgcg ctgaacgccg tcggccagca tccaggccga
     1081 ggcgaaatag tgctgcacca tcgcgaccca gccgtcgctg gcgctgcgct cgtgctcggg
     1141 cttgttgcgg tcggccgcgt cgacgatatc ggcgaacttg aacttctgga aatgcgcggc
     1201 ctgcgtgtac atggccgggc cggtgaaggc ggccgggccg ctgaagaagc tgctgctctg
     1261 cggctggccc tcgggcggct ggccgtcgcg caccagctgc agatagagct gcggctgcac
     1321 cggcgccgtg ccggtgttga tgatctcgtg ggtcacgccg atcacatagt cgccgcgctt
     1381 gagcgtgtag gtcttgcgta agcggacgcc accgacctcg ggcgattcga agacgacgct
     1441 cagcgcgtcg ctgccgggct tcagcgtcgt ttcgccgggc aggaggttca tcaccgtcag
     1501 gtgattgggc agggcgccgg tgttgccgat caggccggtc tgagccaggt agacgcgctc
     1561 gttgccgcgc tcgaacagca cgatgttgcg gttcttgtcg tcctgctgct tctgggtgag
     1621 cagctccagg cgcaccaggt cgccgccgcg gctgtcgaaa gtggccttga ccacgtcgct
     1681 cgccaccgtg acggtctggg ccgcaacggg cgcggaagcg gcggcgcccg gcacggcgga
     1741 aggctggccg ccggccaccg gtgcgggcag accattgccg ggcgcggcgc cggcggcact
     1801 ggccggctgg tggaccgggg agggcgagaa gaaggagggc ttgccgttgt gcttgttcca
     1861 gccgtcccac agcaggaaaa gcgacatcgt gaacaccacc cacagcaggg tgcggcgcat
     1921 atcggtcatg aggacgactc ggttcgggtt ggatcggtaa ggccagcaac ttgcacggcc
     1981 ttcaggcggg agaacaggcg cggcggcgcc tcgggcacgg catcgaggcc gccatcgcac
     2041 cagggatggc agcgcaacag gcgtgcggcg gccagatagc tgccggccgc agcgccatgg
     2101 cggtccaccg cctgcagggc gtagaccgag caggtcggct cgaagcggca ggccgaaccc
     2161 agccaggggc tcagcagcag gcgataggcc ttgatcggca gcatcagcgc gcgccggggc
     2221 cagagctgga gcggggtcat ggcgcgcaca aggcgtggga aaggatcagc gcgccgcgct
     2281 ggcggttttg gccggcagcg agccgaacaa tccggccagc tcctggccca ctgcgcgctt
     2341 gagcgccaca gaggcggcgc tcttgtaggc gctgtcggtg gcatccttgc cggaggagct
     2401 ggagaacggc gcgcgcagac gcagcagcca gtcgccggcc ggcagaacag gcagcgcctg
     2461 cacgaaggcc tggcggccct ggcgcttgat cagattgcgc gtcacggcac ggcgcgcatg
     2521 gcgtttagga acgaccagac ccagccgcca cgccgctggc atggggcttg ctggcctttc
     2581 atccacaacc ggatggcgca tctgctcgac ccctgttgac aacttggtcg ccggcggcgc
     2641 cgaggccgca tgttgcgggc tgaagtgcag gctgaaatgc gggct
//
LOCUS       DASUCY010000263         3525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_776616, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000263 DASUCY010000000
VERSION     DASUCY010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776616"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /locus_tag="VFV25_05030"
     CDS             complement(<1..1065)
                     /locus_tag="VFV25_05030"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY1392713.1"
                     /translation="MRRVYSLMVLLAASVLLLAAPLLGAAGAAPDTPQPATDRPDLAY
                     LKQVNAWRPPADPQLLFLLMAQYANAGRHLEGAAYLDELRRRFDPQLNDTQRALYLTA
                     IASLRAGGARDVFLLRRMGWVRDSLAMLDEAARLTHGQAFITHWMSGVVRAQLPAFFG
                     ERGTAEDELRWCLQHADRMPDPGWLREVHFQLAALARQRGDAATAQRELAASGYAQPS
                     KPVTFTTPYSEDPIGGHTFAGRRIKELVPGSVYLVSGYEFTEYYFVISADRRELMAID
                     AGSRADAAREAHEALRAKLGTLPPLTTVFITHAHWDHVGGQRYFRSLQPAPRFIGRSN
                     YAEELAHDAMGDAGNARHFFG"
     gene            1167..1484
                     /locus_tag="VFV25_05035"
     CDS             1167..1484
                     /locus_tag="VFV25_05035"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY1392714.1"
                     /translation="MARSYGIDDCPVARTLDLIGERWTVLLLRDLLLHGARRFQDFQD
                     SLPGVAPNTLSARLKAMEDDGLVQRVLYSERPLRLEYALTEKGKSLGPVVRAMRDWGL
                     KHH"
     gene            1511..2434
                     /locus_tag="VFV25_05040"
     CDS             1511..2434
                     /locus_tag="VFV25_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY1392715.1"
                     /translation="MASIEGVGKEPTAHLALAAACGAGFLWGTGAVVVNVLIARHGFT
                     PENISFWRFAVGAIVLLAVFGRPGLWEALRPLLPTVVAAGVAMAGYVLCWFLGIERIG
                     AAIPTLIALCLPPVIVTIVALLRGQERLDAGLLVILVAALAGTVLIVARHGMTALGTD
                     GAQVTVGVLCSIASALLYAGFTLVSGRLASRLGPGQATTSLTMVAAVVMSLSALYRPL
                     HWPADVPPQAWFLYLGVVTAALALLAFSWGAARLSPMALTVATLVEPLTAVLLAALFL
                     GERLSALQWLGGALLLASIWGLGRRVTLPHG"
     gene            complement(2470..3255)
                     /gene="fnr"
                     /locus_tag="VFV25_05045"
     CDS             complement(2470..3255)
                     /gene="fnr"
                     /locus_tag="VFV25_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HEY1392716.1"
                     /translation="MHDDAPPTADRPFKCAARVERATVTRLQDLKVACSSCNLRELCL
                     PLGLSSPELDRLDRLIASRRTVKRGEALFRAGDAFQSLYAVRTGFFKTSLSSEDGRDQ
                     VTGFQMAGELLGLDAISQDKHTCDAIALEDSQVCVIPYSQLETVSREFTDLQRSFHKI
                     MSREIVRDHGVMLLLGGMRAEERLAAFLLNLTQRLQARGFSPSSLVLRMTREEIGSYL
                     GLKLETISRTFSKFAEDGLLEVRQRQIRVLDPEGLRRLISSAG"
     gene            3358..>3525
                     /locus_tag="VFV25_05050"
     CDS             3358..>3525
                     /locus_tag="VFV25_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HEY1392717.1"
                     /translation="MNAVTNPPAISEQLLARFDVPGPRYTSYPTADRFVEAYTAAQYQ
                     EALRQRASGMGG"
BASE COUNT          539 a         1144 c         1243 g          599 t
ORIGIN      
        1 gccgaagaaa tgccgcgcgt tgcccgcgtc gcccatcgca tcatgggcca gctcctcggc
       61 atagttgctg cggccgatga agcgcggcgc gggctgcagc gagcggaagt agcgctggcc
      121 gcccacatgg tcccagtgcg catgcgtgat gaacacggtc gtcaggggtg gcagcgtgcc
      181 cagcttcgcg cgcagcgctt cgtgcgcctc gcgtgcggcg tcggcgcgtg agccggcatc
      241 gatcgccatc agctcgcgcc ggtcggccga gatgacgaag tagtactcgg tgaactcgta
      301 gcccgacacg aggtagacgc tgcccggcac cagctctttg atgcgccgcc cggcgaaggt
      361 gtggccgccg atcggatcct cggagtaagg cgtcgtgaag gtcacgggct tgctcggctg
      421 cgcgtagccg ctcgcggcta gttcgcgctg cgcggtggcc gcgtcaccgc gctgccgcgc
      481 cagcgccgcc agctggaagt gcacctcgcg cagccagccc gggtccggca tgcggtcggc
      541 gtgctgcagg caccaacgca gctcgtcctc ggcggtgccg cgctcgccga aaaaggcggg
      601 caactgtgcg cgcacgacac ccgacatcca gtgggtgatg aacgcctgcc cgtgcgtcag
      661 gcgcgccgcc tcgtccagca tcgccagcga gtcgcgcacc cagcccatgc gccgcagcag
      721 gaagacgtcg cgcgcgccgc ccgcacgcag cgatgcgatc gcagtcaagt acagcgcgcg
      781 ttgcgtgtcg ttcagctgcg gatcgaagcg gcggcgcagt tcgtcgagat acgcggcgcc
      841 ttcgaggtgg cggcccgcgt tggcatattg cgccatcagc aggaacagaa gctgcgggtc
      901 cgccggcggc cgccaggcgt tgacttgctt cagataggca aggtcgggtc gatccgtcgc
      961 cggttggggg gtgtccggcg cggcacctgc ggcaccgagc agcggcgccg cgagcagcag
     1021 cacgctggcg gcgagtagaa ccatcaggct gtaaacacgg cgcatcgcgg gccccttctc
     1081 atcgatgatg gcttgcaaaa tgcaagtgac tatcaaaaat atatgcactt gcgaagaaga
     1141 agtcaaatgg gtaagctgca atcacgatgg caagaagcta cggtatcgac gactgcccgg
     1201 tggcgcgcac gctcgacctg atcggcgagc gctggacggt gctgctgctg cgcgacctgc
     1261 tcttgcacgg ggcacgccgc ttccaggact tccaggactc gctgcctggc gtggcgccca
     1321 atacgttgtc ggcgcgcctg aaggcgatgg aagatgacgg gctcgtgcag cgcgttctct
     1381 acagcgagag gccgctgcgg ctggaatatg cgctcaccga gaagggcaag agcctcggac
     1441 cggtggtcag ggcgatgcgg gattggggcc tgaaacatca ttagccaggc tttgggaggc
     1501 actggatcca atggcaagca tcgagggagt cggtaaggaa ccaacggcac atctggcgct
     1561 ggctgcggct tgcggcgcag gtttcctctg gggcaccggc gcagtggtgg tcaacgtgct
     1621 tatcgcgcgt cacggcttca caccggagaa catctctttc tggcggtttg cggtcggcgc
     1681 catcgtgctg ctcgccgtgt ttggccgacc ggggctgtgg gaggcgctgc ggccgctgct
     1741 gccgactgtc gttgcggccg gcgtggcgat ggccggctac gtcctgtgct ggttcctggg
     1801 aatcgagcgc atcggtgcag caatcccaac gctcatcgcc ttgtgcctgc cacctgtgat
     1861 cgtgaccatc gtcgcgttgc tgcgcggcca ggagaggctc gatgcagggc tgcttgtcat
     1921 cctggtcgcc gccctggccg gcacggtgct catcgttgcg cgccacggca tgactgcgct
     1981 cggcaccgac ggagcccagg tgaccgttgg cgtgctttgc tcaattgcgt cggcgctgct
     2041 ctatgctggt ttcacgctgg tcagcggccg gctggcgagt cgcctgggcc cagggcaggc
     2101 gacgaccagt ctgaccatgg tcgctgcagt cgtgatgagc ctgtcggcgc tttacaggcc
     2161 gctgcactgg ccggccgatg tgccgcctca ggcctggttt ctctacctcg gggtcgtaac
     2221 ggcggcgctt gcgctgcttg cgttcagctg gggcgcggcg cggctgtcgc cgatggcgct
     2281 gaccgtcgcg acgctggtcg agccattgac cgcggtgctg ctggccgcgc tcttcctcgg
     2341 cgagcgtttg agcgcattgc aatggctggg aggtgccttg ctgctcgcca gcatctgggg
     2401 gctcgggcgg cgcgtcacgc tgccacacgg ctgactgcgg cccagcctcc gtccaggagg
     2461 ctcagcggct cagcccgcgc tgctgatcag cctgcgcagc ccctccggat cgagcacgcg
     2521 gatctgccgc tgcctgactt ccagcagacc gtcttcggcg aacttggaaa aggtgcggct
     2581 gatcgtctcg agcttgaggc ccaggtagct gccgatctcc tcacgcgtca tgcgcagcac
     2641 cagcgaggag ggcgagaagc cgcgcgcctg caggcgctgg gtcaggttga gcaggaaggc
     2701 ggccaggcgc tcctcggcgc gcatgccgcc cagcagcagc atcacgccgt ggtcgcgcac
     2761 gatctcgcgg ctcatgatct tgtggaagga gcgctgcagg tcggtgaact cgcgcgacac
     2821 cgtctccagc tgactgtagg ggatcacgca gacctgggag tcttccagcg cgatggcgtc
     2881 acaggtgtgc ttgtcctggc tgatcgcgtc caggcccaac aactcgccgg ccatctggaa
     2941 gcccgtgacc tggtcgcgcc cgtcctccga ggacagagag gtcttgaaga agccggtgcg
     3001 caccgcatag agcgattgga aagcgtcgcc ggcgcggaac aaggcctcgc cgcgcttgac
     3061 cgtgcggcgc gaggcgatga ggcgatccag ccggtccagc tccgggctcg acaggccgag
     3121 cggcaggcac agctcgcgca ggttgcagct ggagcaggcg actttcaggt cctgcagccg
     3181 ggttaccgtg gcgcgctcga ctcgcgctgc gcatttgaag ggccggtcgg cggtaggggg
     3241 ggcatcgtcg tgcatatgag gcacctcaag ggtggttgag attgtccggc gtgggtccgg
     3301 ccgatgcttg agctggatca agcctgctgc gcttgtccgc cggcacagtg gcagacgatg
     3361 aacgccgtga ccaacccgcc cgcgatctcc gagcagctcc tggcccgctt cgacgtgccc
     3421 ggcccgcgct acacctccta cccgacggcc gatcgcttcg tcgaggccta taccgccgcc
     3481 cagtaccagg aggccttgcg gcagcgcgcc agcggcatgg gcggc
//
LOCUS       DASUCY010000264         4032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_358780, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000264 DASUCY010000000
VERSION     DASUCY010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358780"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VFV25_05055"
     CDS             complement(<1..594)
                     /locus_tag="VFV25_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392718.1"
                     /translation="MKELVTAVSLALASAAGVAQVVRFDGDAAGSVPAGWRCGVTGKG
                     SPHWAVAADPTAPRAPNVLLQSGSGTFPWCVKNGTSMADGFVEVKFKPISGREDQAGG
                     VVWRWKDGDNYYVARANALENNVSLYYTERGSRKTIKYVDAPVAGGIWHSLRVEFAGA
                     RIAVSLDGKRYIELDDTHIAGAGAVGMWTKADSVTAFD"
     gene            complement(605..1801)
                     /locus_tag="VFV25_05060"
     CDS             complement(605..1801)
                     /locus_tag="VFV25_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEY1392719.1"
                     /translation="MSEATVAEAPKTPAAARYTLWQLVRYMLGLGTWGFGGPVALVGY
                     MYRDLVEKRGWISESDYKEGLTLAQLMPGPMAAQLAIYLGFVHFRIVGATLVGLAFVL
                     PSFLMVVALGAVYVSYGGIGWMQAVFYGVGAAVIGIIAMSAYKLTTKNIGRDKLLWAI
                     FGGNTALTAITQTEFVWVVLGAGVLVWLLRAPPKAWMGGGRVNGVAAPLLGALAFTSA
                     DPHKLAQIGAYFAYAGSFVFGSGLAIVPFLYGGVVKEYGWLTDKQFVDAVAVAMITPG
                     PVVITTGFIGFLVSGFWGAVVAAAGTFLPCYLLTILPAPYFKKHGKRPGLLAFIDGAT
                     AAAIGAIAGSVIVIGQRSITDWLTALMAIVTAIVLWRFKKVTEPVVVVAAAIVGLIAY
                     PLLSHV"
     gene            complement(1825..2796)
                     /locus_tag="VFV25_05065"
     CDS             complement(1825..2796)
                     /locus_tag="VFV25_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011003855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HEY1392720.1"
                     /translation="MDSRQQHWLLLIVSLPTPSATARMRVWRGLKALGCMALRDGAYL
                     LPMGEEREPALRELGDECTREGGVAWLMAVQPRSADEEQAYRQLFDRSDEYAELRQSW
                     KQANRGLAGLSATELTRLQRKLLREYEAVRAIDFFHGDASAEAEAAWTDLNRRIEQLL
                     SPDEPHETAGGIPRLEAQQYRGRTWATRRRLWVDRVASAWLIRRFIDPEARFKWLARP
                     SDCPKSALGFDFDGAAFTHVGDRVSFETLLASFGLEEDPALVRLGAMVHQLDAGGEPV
                     PEAVGFEAVIAGARERLDDDDALLGEMTAVLDSLYAHFAREANRSRT"
     gene            3062..3202
                     /locus_tag="VFV25_05070"
     CDS             3062..3202
                     /locus_tag="VFV25_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392721.1"
                     /translation="MRPLLDTLLDVGRSDVPGSGMAAVIPADRFLHLRTCTEALLVVP
                     SG"
     gene            complement(3331..>4032)
                     /locus_tag="VFV25_05075"
     CDS             complement(3331..>4032)
                     /locus_tag="VFV25_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEY1392722.1"
                     /translation="GEYGWPRVWKELLARGVRVGKERVQKLMQLHGIKARAKRRYKAT
                     TDSRHDLPVAPDLLQRDFSPTAPDCAWSSDITYIATDEGWLYLAVIVDLYSRMVVGWA
                     MQPHMRTSLVADALRMAWFRRQPAPHLIFHSDRGAQYCSHEFQRALKGYGMLSSMSRK
                     GDCWDNAVSESLWGSLKVARIHGRRFGTRRDAMDEVVDWLTFYNHRRLHSSLGYVSPM
                     QFERAWLAAHERRAA"
BASE COUNT          689 a         1397 c         1277 g          669 t
ORIGIN      
        1 gtcgaacgcc gtgacgctgt cagccttcgt ccacatgccg accgcgcccg ccccagcgat
       61 gtgggtatcg tccagttcga tgtagcgctt gccgtccagc gacacggcaa tgcgtgctcc
      121 cgcgaactcc acgcgcaacg agtgccatat gccgccggcc accggcgcgt ccacgtactt
      181 gatagtcttg cggctgccgc gctcggtgta gtacagcgac acgttgttct ccagcgcatt
      241 ggcgcgcgcc acgtagtagt tgtctccatc cttccagcgc cagaccacgc cgccggcctg
      301 gtcctcgcgg cccgagatcg gtttgaactt cacttcgacg aagccgtcgg ccatcgacgt
      361 gccgttcttc acgcaccacg ggaaggtgcc gctgccgctt tgcaacagca cgttcggagc
      421 gcggggcgcg gtcggatccg cagcgacggc ccagtgcggg cttcctttgc cagtgacgcc
      481 gcagcgccag cccgccggca ccgagccggc ggcatcacca tcgaagcgca cgacctgcgc
      541 aacgcccgcg gccgacgcga gcgccagcga aacagcagtg acgagttcct tcatgatctc
      601 tccttcacac gtgcgagagc agcgggtagg cgatcaggcc cacgatcgcg gcggccacca
      661 cgaccaccgg ctcggtgacc ttcttgaagc gccacagcac gatggcggtc acgatggcca
      721 tcagtgcggt gagccagtcg gtgatcgagc gctggccgat cacgatgacc gagccggcga
      781 ttgcgccgat ggccgcggct gtcgcaccgt cgatgaaggc gagcagtccc ggccgcttgc
      841 catgcttctt gaagtacggc gcgggcagga tcgtcagcag gtagcacggc agaaaggtcc
      901 ctgcggccgc gaccaccgca ccccagaagc ccgacaccag aaaaccgatg aagccggtgg
      961 taatcacgac ggggccgggc gtgatcatcg cgaccgcaac cgcgtcgacg aactgcttgt
     1021 cggtgagcca gccgtactcc ttgactacgc cgccgtagag gaagggcacg atggccaagc
     1081 cgctgccgaa cacgaagctg ccggcgtagg cgaaataggc tccgatctgc gccagcttgt
     1141 gcgggtcggc gctggtgaag gccaacgcgc cgagcagcgg cgccgccacg ccgttgacgc
     1201 ggcccccgcc catccaggcc ttgggcggcg cgcgcagcag ccagacgagc acgcccgcgc
     1261 cgagcacgac ccagacgaat tcggtctgcg tgatcgccgt cagcgccgta ttgccgccga
     1321 agatggccca cagcagcttg tcgcggccga tgttcttcgt ggtcagcttg tacgcgctca
     1381 tcgcgatgat cccgatcacc gcggcgccta cgccgtagaa cacggcctgc atccagccga
     1441 tgccgccgta gctcacgtag accgcgccca gcgccaccac cataaggaac gagggcagca
     1501 cgaaggccag gccaaccagc gtggcgccga cgatgcggaa gtggacgaag ccgaggtaga
     1561 tggcgagctg cgccgccatc gggccaggca tcagctgcgc caaggtcagg ccttccttgt
     1621 agtcgctctc ggagatccag ccgcgctttt ccaccaggtc gcggtacatg tagcccacca
     1681 gcgcgaccgg cccgccaaag ccccaggtgc cgaggcccag catgtagcgc acgagctgcc
     1741 atagcgtgta gcgcgctgca gcaggtgtct tgggcgcctc ggccacggtc gcctcactca
     1801 tgcatcttct ccattgggtc taggctaggt cctggatcgg ttcgcttcgc gagcgaagtg
     1861 ggcatagagc gaatcgagca cggccgtcat ctcaccgagc agtgcgtcgt catcgtcgag
     1921 ccgctcgcgc gccccggcta tcacggcctc gaagccgacc gcctcgggaa ccggttcacc
     1981 gcccgcatcg agctggtgaa ccattgcgcc caagcgcacg agcgcgggat cttcttccag
     2041 cccgaagctc gcgagcaagg tctcgaagct gacccggtcg ccgacgtgcg tgaaggctgc
     2101 cccgtcgaag tcaaagccga gcgcgctctt cgggcaatcc gagggccggg ccagccactt
     2161 gaagcgggcc tccggatcga tgaaacgccg gatcagccac gcgctggcca cacggtccac
     2221 ccacaggcgg cggcgcgtgg cccaggtgcg gccgcggtac tgctgcgcct ccaggcgagg
     2281 aataccgccc gcggtctcgt gaggctcatc cggtgacagc aactgctcga tgcggcggtt
     2341 gaggtccgtc caggcggcct cggcctctgc actggcgtcg ccatggaaga agtcgatcgc
     2401 gcgcacggcc tcgtactcac gcagcagctt gcgctgcagg cgtgtcagct cggtggctga
     2461 taggcccgcc aagccgcggt tcgcctgctt ccagctctgg cgcagctcgg cgtactcgtc
     2521 gctgcggtcg aacagctgcc gataggcctg ctcctcgtcg gccgagcgcg gctgtacggc
     2581 catcagccag gcaacgccgc cttcccgggt gcactcgtcc ccgagctcgc gcagcgccgg
     2641 ctcgcgctcc tcccccatcg gcagcaggta ggcgccgtcg cgcaaggcca tgcagccaag
     2701 ggccttgagc cctcgccaca cgcgcatgcg cgccgtcgca cttggtgtcg gaagcgaaac
     2761 gatgagcagc agccaatgct gctgccgcga gtccatatcc atgtctcgaa tataactctg
     2821 ctgtcgcgta tgctacatca tatgaccctt ctacgaatat ttcactgcaa agactcgcca
     2881 aatcggccgc tgtgttgctg ctcgccgtgc tgccattcgc agttactgcc ggcgtgtcgt
     2941 tcgcgggccg cgtcgagttg tcgctgacga aggtgaaggg cttcgcgagt acaagaactt
     3001 cctgaccggc gactggctga gcgatcacac caaggttgag atcttcagcg aggagtcgaa
     3061 agtgcggccg ctgctcgaca ccctgctaga cgtcggccgc tccgatgttc ctggttcggg
     3121 aatggcggcc gtgatcccgg cggatcgctt tcttcacctg cgcacctgta ccgaggcctt
     3181 gctcgtcgtc ccgtcgggct gaacgtcagt cgcgcagcaa actaacgcgg ggaggttctc
     3241 gatgcttcac gcagcccgag caccgcagtc cagatgaatg ggccctacag tgagcttgcc
     3301 ctcgtaaatc ggatcccata accgaggtca tcatgccgct cgcctttcgt gggcggcgag
     3361 ccaggcccgt tcgaactgca tcggactgac gtaacccaat gacgagtgca ggcgtcgatg
     3421 gttgtagaag gtcagccagt caacgacctc gtccatcgcg tcgcgccgcg tgccgaatcg
     3481 cctgccgtgg atgcgtgcaa ccttcagcga gccccacagg ctctcactga ccgcgttgtc
     3541 ccagcagtcg cctttgcggc tcatcgagct gagcatgccg tagcccttga gggcgcgctg
     3601 gaactcgtgg ctgcaatact gagctccgcg gtcgctgtgg aagatcaggt gcggcgccgg
     3661 ctgccggcgg aaccaggcca tgcgcaacgc gtcggcgacc agcgatgtcc tcatgtgcgg
     3721 ctgcatcgcc cagcccacga ccatacggct gtacaggtcg acgatcacgg ccaggtacag
     3781 ccagccttcg tcggtggcga tgtaggtgat gtcggagctc cacgcgcagt ccggcgccgt
     3841 cggcgagaag tcgcgctgca gcaggtccgg tgccaccggt agatcgtggc ggctgtcggt
     3901 ggtcgccttg tacctgcgct tggctctggc cttgatgccg tgcagttgca tgagcttctg
     3961 cacacgctcc ttgcccacgc gcacgccgcg cgccagcagc tccttccaca cgcgcggcca
     4021 gccgtactcg cc
//
LOCUS       DASUCY010000265         3201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_214294, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000265 DASUCY010000000
VERSION     DASUCY010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214294"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1783
                     /locus_tag="VFV25_05080"
     CDS             <1..1783
                     /locus_tag="VFV25_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY1392723.1"
                     /translation="MGMLLSLAIAFVVTPWLARLWMKPHAAAAHGGAPKGLAARLSPL
                     FERIFRPLLDEHRGTRNRAVLGAGVAGLIAVSLALPALGLVVLKMLPFDNKSEFQVVV
                     DMPAGTPVERTAAVLRELGAYLARQPEVTDYQAYAGTAAPINFNGLVRQYYLRAGGEV
                     GDLQVNLVGKQARKDQSHAIVTRLRPALQAIGHRYGANVKLVEVPPGPPVLSPIVAEI
                     YGPDADGRRSVAKAVRAVFEQTPGVVDVDDSSIAAAPRKLLLVDRRKAAMLGVAQADI
                     VTTLRAGLAGEPASYLHDESKYPAAATLQLPPERQGDLDALLQLAVRGAGGQLVPIRE
                     LVTVSDAQREQPIFHKDLLPVSYVVGDMAGALDSPLYGMFKMRGALRDIATPGGGRLG
                     EYFIRQPDDAWRDYAIKWDGEWQITYETFRDMGAAYAVGLVLIYLLVVAQFASYLTPL
                     IIMAPIPLTIVGVMPGHALLGAQFTATSMIGMIALAGIIVRNSILLVDFIKLQVAQGI
                     ALKDAVVRSAATRAQPILLTGLAAMLGALFILDDPIFNGLAISLIFGILVSTMLTLVV
                     IPLLYFAAYRRGQAATLPVTSPQGAST"
     gene            1780..1989
                     /locus_tag="VFV25_05085"
     CDS             1780..1989
                     /locus_tag="VFV25_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HEY1392724.1"
                     /translation="MTSWQLVRMFAGLFVLLSLALGVPGSPLYLSSWWLAFTAFVGAN
                     LLQSAFTRWCPLEIILRKLGVEPGC"
     gene            2004..2426
                     /gene="trxC"
                     /locus_tag="VFV25_05090"
     CDS             2004..2426
                     /gene="trxC"
                     /locus_tag="VFV25_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HEY1392725.1"
                     /translation="MHLVCPDCGTKNRVPPQRLHEQPQCGQCGAPLMAPQPAALNEAT
                     FERFVEGTELPVLVDFWAEWCGPCRMMAPQFAAAAQQLPEVRFAKVDTEASPRTAVRN
                     RIRSIPTLVLFHRGREIARHSGATSAAEILAWLRSHPL"
     gene            2521..>3201
                     /locus_tag="VFV25_05095"
     CDS             2521..>3201
                     /locus_tag="VFV25_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY1392726.1"
                     /translation="MAHIVILGAGIGGMPAAYELRAALDRSHRITVVNAVDYFQFVPS
                     NPWLAVGWREREAITFPIHPYLEKRGIGFVAQRVQRIDAAASALDLQDGTRIGYDYLV
                     ITTGPKLSFDEVPGAGPEHHTQSICTVDHAQQAYEAYQGFLQEPGPVIIGAMPGASCF
                     GPAYEFAFIFNKDLQRRKLRHKVPITFVTSEPYIGHLGLGGVGDSKSMLESEFRNSDI
                     RWITNAKVT"
BASE COUNT          497 a         1131 c         1052 g          521 t
ORIGIN      
        1 catgggcatg ctgctgtcgc tggccatcgc cttcgtagtc acgccctggc tcgcccggct
       61 gtggatgaag ccgcacgcgg cggctgcgca tggcggcgca cccaaagggc tggccgcacg
      121 gctcagcccc ctgttcgagc gcatcttccg cccgctgctc gatgagcacc gcggcacccg
      181 caaccgcgcg gtgctgggcg caggggtagc cggcttgatc gccgtgtcat tggcgctgcc
      241 ggcgctgggg ctcgtggtgc tgaagatgct gcccttcgac aacaagtcgg agtttcaggt
      301 ggtggtcgac atgccggccg gcacgccggt tgaacggacc gcggccgtgc tgcgcgagct
      361 gggtgcctac ttagcacgcc agccggaggt caccgactac caggcctatg ccggcaccgc
      421 ggcgccgatc aatttcaacg gcctggtgcg ccagtactac ttgcgcgccg gcggcgaggt
      481 gggcgatctg caggtgaacc tggtcggcaa acaggcgcgc aaggaccaga gccacgcgat
      541 cgtgacgcgg ctgcgccccg cgctgcaggc catcggccac cgctacggcg ccaatgtgaa
      601 gctggtcgag gtgcctccgg gcccgccggt gctgtcgccc atcgtcgccg agatctatgg
      661 cccggacgcc gacggacggc gcagcgtcgc caaggcggtg cgcgcggtgt tcgagcagac
      721 gcctggcgtg gtcgacgtgg acgacagcag catcgccgcc gcgccgagga agctgctgct
      781 ggtcgatcgc cgcaaggcgg caatgctcgg cgtggcgcag gccgacattg tgacgacgct
      841 gcgcgccggg ctggcaggcg agccggcctc ctacctgcac gacgaaagca agtacccggc
      901 cgccgcgacg ctgcagctgc cgcccgagcg ccagggcgac ctcgacgcgc tgctgcagct
      961 ggccgtgcgc ggcgccggcg gccagctggt gccgatccgc gagctggtga cggtgagcga
     1021 cgcgcagcgc gagcagccga tctttcacaa ggacctgctg ccggtgagct acgtggtcgg
     1081 tgacatggcc ggcgcgctgg acagcccgct gtacggcatg ttcaagatgc gcggcgcgct
     1141 gcgcgacatt gccacgccgg gcggcggcag gctgggcgag tacttcatcc gccagcccga
     1201 cgacgcctgg cgcgactacg ccatcaagtg ggacggtgag tggcagatca cctacgagac
     1261 cttccgcgac atgggcgcgg cctatgcggt gggcctggtg ctgatctacc tgctggtggt
     1321 agcgcaattt gccagctacc tgacgccgct gatcatcatg gcgccgattc cgctgaccat
     1381 cgtcggcgtg atgccaggcc atgcgctgct gggcgcacag ttcaccgcca ccagcatgat
     1441 cggcatgatc gcgctggccg gcatcatcgt gcgcaactcc atcctgctgg tcgacttcat
     1501 caagctgcag gtcgcccaag gcatcgcgct caaggacgcc gtggtgcgct cggccgccac
     1561 gcgcgcgcag cccatcctgc tgactggcct ggccgccatg ctgggcgccc tcttcatcct
     1621 cgacgacccg atcttcaacg ggctggccat ctcgctgatc ttcggcatcc tggtctccac
     1681 catgctgacg ctggtggtga tcccgctgct gtatttcgcc gcctatcggc gcggccaggc
     1741 ggccaccctc cccgtcacct caccccaagg agcatccaca tgacttcgtg gcaactcgtg
     1801 cgcatgttcg ccggcctctt cgtcctgctg tcactggcac tcggcgtccc gggcagcccg
     1861 ctgtacctca gcagctggtg gctggccttc acggccttcg tcggcgccaa cctgctgcaa
     1921 agcgcgttca cgcgctggtg tccgctggag atcatcctgc gcaagctcgg cgtggagccg
     1981 ggttgctgag ccgggggcgc gccatgcatc tcgtctgtcc cgactgcggc acgaagaatc
     2041 gcgtgccgcc gcagcgcctg cacgagcagc cgcaatgcgg ccaatgcggc gcgccgctca
     2101 tggcgccgca gccagcggcg ttgaacgaag ccacgttcga gcgcttcgtc gaaggcaccg
     2161 agctgccggt actggtggac ttctgggctg aatggtgcgg cccctgccgg atgatggcac
     2221 cgcagttcgc cgccgccgcg cagcagctgc ccgaggtgcg cttcgccaag gtcgacaccg
     2281 aggcgtcgcc ccgcaccgcc gtgcgcaacc gcatccgcag catcccgacg ctggtgctat
     2341 tccatcgcgg ccgcgagatc gcgcggcaca gcggcgcgac gagcgccgcc gagatcctgg
     2401 cctggctgcg cagccatccg ctctgaaggc ccggttgcgt gaagcacgct tgtcgcagat
     2461 caatgccgtg ctgcaggtat cacgctaaga ctcatctgcc cacctcacga ggagaccatc
     2521 atggctcaca tcgtcatcct gggtgcaggc atcggcggca tgccggccgc ctacgagctg
     2581 cgcgccgcgc tcgatcgctc gcaccgcatc accgtggtca atgcggtgga ctacttccag
     2641 ttcgtgccgt ccaacccctg gctagcggtg ggttggcgcg aacgagaggc gatcaccttc
     2701 ccgatccacc cctacctgga gaagaggggc atcggcttcg tcgcgcagcg cgtgcagcgt
     2761 atcgacgccg cggccagcgc cctcgatctg caggacggaa cacgcatcgg ctacgactac
     2821 ctcgtcatca ccaccggccc caagctgtcg ttcgacgagg tgcctggcgc aggccccgag
     2881 caccatacgc agtcgatctg cacggttgac cacgcacagc aggcctacga ggcctaccag
     2941 ggattcctcc aagagccggg gccggtcatc atcggcgcga tgcccggcgc ctcctgcttc
     3001 gggccggcct atgagtttgc ctttatcttc aacaaggatc tgcagcgccg caagctgcgc
     3061 cacaaggtgc ccatcacctt cgtcaccagc gagccctata tcggccacct ggggctgggc
     3121 ggcgtcggcg attcgaagtc catgctggaa agcgaattcc gcaacagcga catccggtgg
     3181 atcaccaacg ccaaggtcac g
//
LOCUS       DASUCY010000266         2708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_21644, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000266 DASUCY010000000
VERSION     DASUCY010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21644"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="VFV25_05100"
     CDS             complement(<1..852)
                     /locus_tag="VFV25_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY1392727.1"
                     /translation="MSEFYDALETRDPEQREAELLAALPAQITHAQVRTTAFAESLAG
                     IDARAISSRAALATLPVIRKHELLERQQARRNEDVFGGFSAIGWRGLRHARGAKRVFQ
                     SPGPIYEPEGEASDYWRMARAMHAAGFRAGDLIHNSFSYHLTPAGSMMETGAHALGCT
                     VFPGGVGNTELQLQAMLELKPQGYAGTPSFLKILLDKAGEQGLAPLSLKKSLVSGEAF
                     PPSLRDAVRALGVEAYQCYATADLGLIAYETEAREGLVLDERVIVEIVRPGSGEPVPA
                     GEVGEVVV"
     gene            complement(881..1177)
                     /locus_tag="VFV25_05105"
     CDS             complement(881..1177)
                     /locus_tag="VFV25_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392728.1"
                     /translation="MEDNGFFAPPPFKPEEALIQLKRSLRDLRAFNERGSGFELKGQA
                     VIELALDGAQIHARIAKRPARSPEWETRALKNSADVRQFIADVKKRLANWSSDE"
     gene            complement(1189..1998)
                     /locus_tag="VFV25_05110"
     CDS             complement(1189..1998)
                     /locus_tag="VFV25_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY1392729.1"
                     /translation="MSAPPLLNVNGIEVIYNHVILVLKGVSLQVREGGIVALLGGNGA
                     GKTTTLRAVSNLLAGERGEVTKGSIELRGERIEKLSTADMVERGVVQVMEGRHCFGHL
                     TIEENLMTGAYTRKDGKAAVAQTLEKVYNYFPRLKTRRGSQAAYTSGGEQQMCAIGRA
                     LMANPKMVLLDEPSMGLAPQIVEEVFEIVRDLNARERVTFLLAEQNTNIALRYADYGY
                     ILENGRIVMDGGAKALRENEDVKEFYLGMGGAQRKSFRDVKSYRRRKRWLA"
     gene            complement(2098..>2708)
                     /locus_tag="VFV25_05115"
     CDS             complement(2098..>2708)
                     /locus_tag="VFV25_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY1392730.1"
                     /translation="STAIKEAQATGYPREKMYGVWWSGAEPDVKDVGEGAKGYNAVAM
                     QHGAEPNSKFVKDVLAQVHAKGQGTGPKEEVGSVLYMRGAISAMLGVEGVKRAQERYG
                     KGKVMTGEQVRWGLENLALDQKKLDALGFAGVMRPVSTSCVDHLGANWARVHTWDGTK
                     WNFSSDWLQADEQIIKPLVKAAADKYAADKKLTRRTPQDCQS"
BASE COUNT          370 a          932 c          893 g          513 t
ORIGIN      
        1 cacgaccacc tcgccgactt cgccggcggg cacgggctcg ccgctgccgg ggcgcacgat
       61 ctcgacgatg acgcgctcgt ccagcaccag cccctcgcgc gcctcggtct cgtaggcgat
      121 caggcccagg tcggccgtgg cgtagcattg gtaggcttcg acgcccaacg cgcgcaccgc
      181 atcgcgcagg ctgggtggaa aggcctcgcc gctcaccagg gacttcttca aggagagtgg
      241 cgccaggccc tgctcgcccg ccttgtccag caggatcttg agaaagctgg gcgtgcctgc
      301 gtaaccctgg ggcttgagtt cgagcatcgc ctgcagctgc agctcggtgt tgccgacgcc
      361 gccgggaaag acagtgcagc ccagcgcatg ggcgcccgtc tccatcatcg agccggccgg
      421 tgtcaggtgg tagctgaagc tgttgtggat caggtcgccg gcgcgaaagc ctgccgcatg
      481 catcgcgcgc gccatgcgcc agtagtcgct cgcctcgccc tcgggctcgt agatcgggcc
      541 gggcgactgg aacacgcgct tcgcgccgcg cgcatggcgc aggccgcgcc agcctatggc
      601 cgagaagccg ccgaagacat cctcgttgcg ccgcgcctgc tggcgctcca gcaattcatg
      661 cttgcgtatc accggcaagg tggccagagc cgcacggctg ctgatcgcgc gtgcgtcgat
      721 gccagcgagg gattcggcga aggcggtagt cctgacctgc gcgtgggtga tctgtgccgg
      781 caaggcggcc agcagctcgg cctcgcgctg ctcggggtcg cgcgtttcca gcgcgtcgta
      841 gaactcgctc atggctgcgt ggtcttggct tggcgttgcg tcattcgtca ctgctccagt
      901 tggcgaggcg cttcttcacg tcggcgatga actggcgcac gtcggcactg ttcttgagcg
      961 cgcgcgtctc ccactcgggg ctgcgcgccg ggcgcttggc gatgcgggcg tggatctgcg
     1021 cgccatccag cgccagctcg atcaccgcct ggcccttgag ctcgaagccg ctgccgcgct
     1081 cgttgaaggc gcgcaggtcg cgcagcgagc gcttgagttg gatcagcgcc tcctcgggct
     1141 tgaagggcgg cggtgcaaag aaaccgttgt cttccatcgt ggcctccttc aggctagcca
     1201 gcgcttgcgg cgcctgtagc tcttcacgtc gcggaagctc ttgcgctgtg cgccgcccat
     1261 gccgaggtag aactccttca catcctcgtt ctcgcgcagg gccttcgccc cgccgtccat
     1321 cacgatgcgg ccgttttcca ggatgtagcc gtagtctgcg tagcgcagcg cgatattcgt
     1381 gttctgctcg gccagcagga aggtcacgcg ctcgcgggcg ttgaggtcgc gcacgatctc
     1441 gaacacttcc tcgacgatct gcggcgccag gcccatcgag ggctcgtcca gcagcaccat
     1501 cttggggttg gccatcagcg cgcggccgat cgcgcacatc tgctgctcgc cgcccgaggt
     1561 gtaggcggcc tgcgaaccgc ggcgcgtctt gagacgaggg aaatagttgt agaccttctc
     1621 cagcgtctgc gcgacggccg ccttgccgtc cttgcgggta taggcgccgg tcatcaggtt
     1681 ctcttcgatg gtgaggtggc cgaagcaatg gcggccttcc atcacctgca ccacgccgcg
     1741 ctccaccatg tcggcggtgc tgagcttctc gatgcgctcg ccgcgcaatt cgatgctgcc
     1801 cttggtgacc tcgccgcgct cgccggccag caggttgctc acggcgcgca gcgtggtcgt
     1861 cttgccggcg ccgttgccgc ccagcagcgc cacgatgccg ccttcgcgca cctgcagcga
     1921 gacgcccttg agcacgagga tcacgtggtt gtagatgacc tcgatgccgt tgacgttgag
     1981 aagaggggga gcgctcacgt attggccttt cttcactcac acaagcgccg cgcgcggtgc
     2041 ttgtgtgagg gctccagcgg agcccatccc tcccctgggg gaggggaagg agaggggtca
     2101 gctctgacag tcttgaggcg tgcgcctggt gagcttcttg tccgccgcgt acttgtcggc
     2161 cgctgccttc accagcggct tgatgatctg ctcgtcggcc tgcagccagt cggagctgaa
     2221 gttccacttc gtgccgtccc aggtgtgcac gcgggcccag ttggcgccca ggtggtcgac
     2281 acagctggtg ctgaccgggc gcatcacgcc ggcgaagccc agcgcgtcaa gcttcttctg
     2341 gtcgagcgca aggttctcca ggccccagcg cacctgctcc ccggtcatca ccttgccctt
     2401 gccatagcgc tcctgtgcgc gcttgacacc ttcgacgccc agcatcgcgg agatggcgcc
     2461 gcgcatgtag agaaccgagc cgacttcctc cttcggtccg gtgccctggc ccttggcgtg
     2521 cacctgcgcc agcacgtcct tcacgaactt ggagttcggc tccgccccgt gctgcatggc
     2581 gacggcgttg tagcccttgg cgccttcgcc cacgtccttc acgtccggct cggcgcccga
     2641 ccaccacacg ccgtacatct tctcgcgcgg gtagccggtg gcctgggctt ccttgatggc
     2701 ggtggagt
//
LOCUS       DASUCY010000267         2795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_117845, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000267 DASUCY010000000
VERSION     DASUCY010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117845"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="VFV25_05120"
     CDS             <1..1035
                     /locus_tag="VFV25_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEY1392731.1"
                     /translation="GAYVPAMSDETIIVKNQGTIFLGGPPLVKAATGEVVSAEDLGGG
                     DVHPRRSGVADHLANNDTHALALARNVVATLNWRKDIPLHCVEPRAPKYAVEELHGVI
                     PTDTRKPYEVREVIARIVDASEFDEFKARYGTTLVCGFAHIEGMPVGIVANNGILFAE
                     SANKGAHFIELCCQRKVPLVFLQNITGFMVGRKYENEGIARAGAKMVTAVACAQVPKF
                     TVIIGGSFGAGNYGMCGRAYSPRFLWMWPNARISVMGGEQAASVLATVKRDGIEAKGG
                     SWSAAEEEAFKAPIRQQYEDQGHPYYASARLWDDGVIDPADTRRVLALGLSASLNAPV
                     PEAKFGVFRM"
     gene            1046..1825
                     /locus_tag="VFV25_05125"
     CDS             1046..1825
                     /locus_tag="VFV25_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY1392732.1"
                     /translation="MSTLDITRSGAVARVYLNRPDVRNAFNDEVIAELTAAFRSLGAD
                     ASLRAIVLGGHGKAFCAGADLSWMKAMAGYSWEQNRADAQGLADMLWTLYSCPLPIVG
                     RIHGDCYAGGVGLASVCDVLVAAEGVHFCLSEAKLGLLPATIGPYVVKALGEQAARRY
                     FITAERFSAARAHALGFVHEVASAEQLDAKVDEIVRALVANGPQAVKACKRLVQDLAG
                     QPVTEALRADTARRIADIRASDEGCEGVRSFLEKRSPKWAG"
     gene            1855..2487
                     /locus_tag="VFV25_05130"
     CDS             1855..2487
                     /locus_tag="VFV25_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HEY1392733.1"
                     /translation="MPTTTTLDTTQLIALAAALGWASGLRLYAVLAITGLAGYAGWVP
                     LPAGLHVLQHPLVIAASCFMVFVEFFADKIPGLDSIWDLVHTAIRIPAGAALAAAVFG
                     GLPGADNATWATIAALLGGSLAATSHVAKATTRAAINTSPEPFSNIGMSLLGDLTVPG
                     TMWLAFHHPIALLVFIVAAVVVMVLLILVLAKYLKAVAHRLSLLFSAVFA"
     gene            2647..>2795
                     /locus_tag="VFV25_05135"
     CDS             2647..>2795
                     /locus_tag="VFV25_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEY1392734.1"
                     /translation="MFKKILIANRGEIACRVAATAHRLGIKTVAVYSEADANAKHVAV
                     CDESVL"
BASE COUNT          456 a          951 c          951 g          437 t
ORIGIN      
        1 ggcgcctatg tgccggcgat gagcgacgag accatcatcg tcaagaacca gggcaccatc
       61 ttcctcggcg gcccgccgct ggtgaaggcc gccaccggcg aggtggtgag cgccgaggac
      121 ctgggcggcg gcgacgtgca cccgcggcgt tctggcgtgg ccgaccatct cgccaacaac
      181 gacacccacg cgctcgcgct ggcgcgcaat gtcgtcgcca cgctcaactg gcgcaaggac
      241 atcccactgc attgcgtcga gccgcgcgcg ccgaagtacg cggtggaaga actgcacggt
      301 gtgatcccca ccgacacgcg caagccctat gaagtgcgcg aggtgatcgc gcgcatcgtc
      361 gatgccagcg agttcgacga gttcaaggcg cgctacggca cgacgctggt gtgcggcttc
      421 gcgcacatcg agggcatgcc ggtgggcatc gtcgccaaca acggcatcct gttcgccgag
      481 agcgccaaca agggcgcgca tttcatcgag ctgtgctgcc agcgcaaggt gccgctggtg
      541 ttcttgcaga acatcaccgg cttcatggtg ggccgcaagt acgagaacga aggcatcgcc
      601 cgcgccggcg ccaagatggt cacggcggtg gcctgcgccc aggtgcccaa gttcacggtc
      661 atcatcggcg gcagcttcgg cgccggcaac tacggcatgt gcggccgcgc ctactcaccc
      721 cgcttcctgt ggatgtggcc caacgcgcgc atcagcgtga tgggcggcga gcaggccgcc
      781 agcgtgctcg ccaccgtcaa gcgcgatggc atcgaggcca aaggcggcag ctggagcgcg
      841 gccgaggaag aggccttcaa ggcgccgatc cgccagcagt acgaagacca ggggcacccc
      901 tactacgcga gcgcgcggct gtgggacgac ggcgtgatcg atccggccga cacaaggcgg
      961 gtattggcgc tgggcctgag cgcgagtctc aacgcgccgg tgcccgaagc gaaattcggc
     1021 gtgttccgca tgtgaggtgg aggcgatgag cacactcgac atcacccgca gcggcgcggt
     1081 cgcccgcgtc tacctcaacc gcccggatgt gcgcaacgcc ttcaacgacg aggtgatcgc
     1141 cgagctcacg gccgcgttcc ggtctctggg cgcggatgcc agcctgcgcg ccatcgtgct
     1201 cggcggccac ggtaaggcct tctgcgccgg ggccgacctg tcgtggatga aggcgatggc
     1261 cggctacagc tgggaacaga accgcgccga tgcgcagggc ctggccgaca tgctgtggac
     1321 gctctacagc tgcccgctgc ccatcgtcgg ccgcatccac ggcgactgct atgccggcgg
     1381 cgtggggttg gcttccgtct gcgacgtgct ggtcgcggcc gagggcgtgc acttctgcct
     1441 cagcgaggcc aagctcggcc tgctgccggc caccatcggc ccctatgtgg tgaaggcctt
     1501 gggcgagcag gcggcgcggc gctacttcat caccgccgag cgcttctcgg cggcgcgcgc
     1561 ccatgccctg ggcttcgtgc acgaggtggc aagcgccgag caactcgacg ccaaggtcga
     1621 cgagatcgtg cgggcgctgg tggccaacgg cccgcaggcc gtcaaggcct gcaagcgcct
     1681 ggtgcaggat ctcgcaggcc agccggtgac cgaggcgctg cgcgccgaca cggctcgccg
     1741 catcgccgac atccgcgcca gcgacgaggg ctgcgagggc gtgcgctcct tcctcgagaa
     1801 gcgcagcccc aagtgggcag gctaagctgg ccccgccagc aggaggacgt tgatatgccg
     1861 accacgacca cgctcgacac cacgcagctg atcgcgctgg ccgccgccct gggctgggcc
     1921 agcggcctgc gcctctacgc tgtgctggcc atcaccggcc tggcgggcta tgcgggctgg
     1981 gtgccgctgc ctgccggcct gcatgtgctg cagcacccgc tggtgatcgc cgccagctgc
     2041 ttcatggtct tcgtcgagtt cttcgccgac aagattcccg gtctcgactc gatctgggac
     2101 ctggtgcata cggcgatacg cataccggcc ggcgcggcgc tggcggcggc cgtgttcggc
     2161 ggccttcctg gtgcagataa cgcgacctgg gccacgatcg cagccttgct cggcggctcg
     2221 ctcgcggcca ccagccatgt ggccaaggcc accacgcgcg cggcgatcaa cacctcgcca
     2281 gagcctttct ccaacatcgg catgtccctg ctcggcgatc tcacggtgcc ggggacgatg
     2341 tggctggcct tccatcaccc gattgcgctg ctggtcttca tcgtggccgc cgtcgtggta
     2401 atggtgctgc tgatcctggt gctggcgaaa tacctcaagg cggtcgcgca ccggctgagc
     2461 ttgctgttca gcgcggtctt cgcatgacgg gacgaacgaa tgttcaagaa aattctgatc
     2521 gctaaccgcg gagacaaccg cagcgcagcg gaggttgcgg cgaagactca tctgcgtcag
     2581 caggtgagtc gcagccaaac tgccaggcac gcgcagcgtg cggcgatcga gccgcgggag
     2641 tcctgaatgt tcaagaagat cctcattgcc aaccgcggcg agatcgcctg ccgcgtcgcc
     2701 gcgaccgccc atcgtctcgg catcaagacc gtcgccgtgt attcggaagc ggatgccaac
     2761 gccaagcatg ttgccgtctg cgacgagtcg gtgct
//
LOCUS       DASUCY010000268         2084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1387117, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000268 DASUCY010000000
VERSION     DASUCY010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1387117"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VFV25_05140"
     CDS             complement(<1..261)
                     /locus_tag="VFV25_05140"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY1392735.1"
                     /translation="MNETKKRDLKAGVAIAGLADGAMLPGQIDGEDAVLLRQGEALYA
                     IGGQCSHYHAALAEGLLSGHVLHCPLHHAQFDIRSGEALCAPA"
     gene            431..1282
                     /locus_tag="VFV25_05145"
     CDS             431..1282
                     /locus_tag="VFV25_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY1392736.1"
                     /translation="MVGSTAFVMAGGGSLGAVEVGMLRELLAWGVAPSFVVGASAGAI
                     NGAYFAAFPTPEGVAKLQALWSGLKRRELFPLNMGSVMRLLTRRAHLVDAQGLRRLLE
                     RHLPYSQLQDAALPIHIVASNMVTGEEVLLSSGPAVDAVLASAAIPGVFPPVSLNGQM
                     LVDGGVANNTPISTAVRLGAKRVIVLPTGFACALKQLPSGAIGRAMHALSLLVARQLV
                     QDAERYASMVELRIVPSLCPLEYSPYDYSAASILIERAAATTRRWLESGGLEREGAPL
                     ELQPHSH"
     gene            complement(1392..>2084)
                     /locus_tag="VFV25_05150"
     CDS             complement(1392..>2084)
                     /locus_tag="VFV25_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEY1392737.1"
                     /translation="ASADAVHNASTRTALIAGASGLIGRELLRQLAEDALYSEVHVLA
                     RRPLVQAQAMAQAKLHTLIAPLETLSLAPRVNDVYLALGTTIKQAGSQAAFRAVDLEL
                     MLRLARLAREAGAQRLAVVSALGADAHSRIFYNRVKGEMEAGIVALGFESLVLARPSI
                     LAGDRASLQQPARPAEALSLKLLGPIAHWLPAGWRPIAADVVARAMIRALRDAKPGVS
                     VLESARLQELGA"
BASE COUNT          306 a          711 c          731 g          336 t
ORIGIN      
        1 cgccggcgcg cacagcgcct cgccgctgcg gatgtcgaac tgcgcatggt gcagcgggca
       61 atgcagcacg tgaccgctga gcaggccttc ggcgagcgcc gcgtgatagt ggctgcattg
      121 cccgccgatg gcgtagagcg cctcgccctg gcgcagcagg acggcgtctt cgccgtcgat
      181 ctggcccggc agcatggcac cgtcggcaag gcccgcgatc gccacgccgg ccttcaggtc
      241 gcgctttttg gtctcgttca cgggggcctc ctcttcagag atccatgctc gccgcatgcg
      301 caaggcagtc cacgataccc gcggcgcgtg ccggcagcaa aagtaggcct gcacccgaca
      361 gggcctcact cggaaggcgc gtgcgggttg acaatggccc ccgctctgct cagaacgcgg
      421 atccagcgac atggtgggaa gcactgcttt cgtgatggcc ggcggcggca gcttgggcgc
      481 tgtcgaggtc ggcatgctgc gcgaattgct ggcctggggg gtggcaccca gcttcgtggt
      541 cggcgcatcg gcgggcgcga tcaacggcgc ctatttcgcc gcctttccca cgcccgaagg
      601 ggtagccaag ctgcaggcct tgtggagcgg gctgaagcgg cgcgagctct ttccgctcaa
      661 tatgggcagc gtgatgcgcc tgctgacgcg gcgcgcacat ctggtcgatg cccaggggct
      721 gcggcgcctg ctcgagcggc atcttcccta ttcgcagctg caggacgcgg cgcttcccat
      781 ccatatcgtc gcgtccaata tggtgaccgg cgaagaagtg ctgctgtcca gcggccccgc
      841 cgtggacgcg gtgctggcca gcgccgccat ccccggcgtg tttccgccgg tcagcctcaa
      901 cgggcagatg ctggtcgacg gtggcgtagc gaacaacacg ccgatttcca ccgcggtgcg
      961 cttgggagcc aagcgcgtca tcgtcttacc gaccggtttc gcctgcgcgc tcaagcagct
     1021 gccttcgggc gccatcgggc gtgccatgca tgcactcagc ctgctggtgg cgcggcagct
     1081 ggtgcaggac gcggaacgct acgcctcaat ggtggagctg cgcatcgtgc ccagcctgtg
     1141 tccgctcgaa tattcaccgt atgactattc cgccgcttcc atcctcattg agcgggccgc
     1201 ggcaacaacg aggcgctggc tcgaaagcgg cggtctggag cgggagggcg cgcccttgga
     1261 gctgcagccc cacagccatt gagcgcccgt ttctcaagga aacgccgggt cgccccaagg
     1321 tttccttgac ccccgcgggg ggcgggctgg gcgcagcccg gccctggagg tgctcagcat
     1381 actctggctc ctcaggcccc aagctcttgc aggcgcgccg attcgagcac gctcacgccg
     1441 ggctttgcat cacgcagcgc gcggatcatc gcgcgcgcca ccacatcggc ggcgatcggc
     1501 cgccagccag cgggcagcca gtgcgcgatg gggccgagca gcttcaggct gagcgcttcg
     1561 gcaggccgcg ccggctgctg caggctggcg cggtcgcccg ccaggatcga ggggcgcgcg
     1621 agcacgaggc tttcgaagcc gagcgccacg atgcctgctt ccatctcgcc cttgacgcgg
     1681 ttgtagaaga tgcgcgagtg cgcatcggcg cccagcgccg agaccacggc aaggcgctgc
     1741 gcaccggcct cgcgggccag acgcgcaagc ctcagcatca gctccaggtc gacggcgcgg
     1801 aaggccgcct gcgagccggc ctgcttgatc gtcgtgccca gggccagata gacgtcgttg
     1861 acgcgcggcg cgagcgacaa ggtctcgagc ggcgcgatca gcgtgtgcag cttggcctgt
     1921 gccatggctt gcgcctgcac gagcgggcgg cgggccagca catgcacttc gctgtagagc
     1981 gcgtcttcgg caagctggcg cagcagctcg cggccgatga ggccgctggc gcctgcgatc
     2041 agcgccgtgc gcgtgctggc gttgtgaaca gcatcggctg aggc
//
LOCUS       DASUCY010000269         2570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_535775, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000269 DASUCY010000000
VERSION     DASUCY010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535775"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VFV25_05155"
     CDS             <1..195
                     /locus_tag="VFV25_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEY1392738.1"
                     /translation="LKACLGALSGEQQQSLALAFYQGLSHAEVAAHLRQPLGTVKSWL
                     RRGLQALKACLERAAQAAKA"
     gene            205..915
                     /locus_tag="VFV25_05160"
     CDS             205..915
                     /locus_tag="VFV25_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEY1392739.1"
                     /translation="MDYGRPALADRLAAEYALGTLRGGARRRFESLLAAHPMLRQAVR
                     DWQARLMPLSGTVQPIQPSPAVWQRVEQRLFGDGVGMTIASPRRWWQRLGLWQGAAGL
                     AAVAAIGLGLLLSQPQPTQPPIVIVMAANPQAGAGAIPAASFVASVSADGRALVLKPI
                     GAITLDASRQALELWAVPSHGAPRSLGLVSPQQATTVLRAHLLQDTAAFAVSMEPPGG
                     SPTGAPTGPILSLGKLRS"
     gene            1015..1620
                     /locus_tag="VFV25_05165"
     CDS             1015..1620
                     /locus_tag="VFV25_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012838081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEY1392740.1"
                     /translation="MTNHFSTRLAAPHQDPLVAPRRGLLKGAGLLSAGALLMLAGQPG
                     RAQGMGGSEADVNILNVALTLEHEAINAYQLGAGSGLLQKPVLDVAVRFQSDHKSHRD
                     ALVATIRKLGGRPVEEMKLEQYAKQLNAASLKSQTDVLDLAARLELGATNAYLGVIPS
                     FKDAQLAKVAGRLAADETMHWTLLNNALGRPLPGGALSFGA"
     gene            complement(1642..>2570)
                     /gene="waaF"
                     /locus_tag="VFV25_05170"
     CDS             complement(1642..>2570)
                     /gene="waaF"
                     /locus_tag="VFV25_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEY1392741.1"
                     /translation="ALPWVAPVLRAMPQVAEVIELPFAHGRLDWAARKAMAATLRGRF
                     ERAYVLPNSIKSALLPWLARIPRRLGYQGEGRWLLLNQRLANPPRGQERPPMAAYYGA
                     LAGRTASKLKPQLSLPPAQVEAVLQRVGVSRRAYWVFAPGAEYGPAKRWPSEHYAALA
                     RQLFAQSRQPVLLLGSAKEGEICEEISLLAPGACHVMAGRTTLDEAMALIAGARGMVS
                     NDSGLMHVAAAFGIPQAAVFGSTSPEHTPPLSSQARVVWLKSELGLDCMPCFERTCRF
                     GHYRCLTEVQPARVAEALRDALGEVPASTTPL"
BASE COUNT          383 a          938 c          903 g          346 t
ORIGIN      
        1 ctcaaggcct gcctgggcgc gctcagcggc gagcagcagc aaagcctggc gctggccttt
       61 tatcagggcc tgtcgcacgc cgaggtggcg gcgcatctgc gccagccgct gggcacggtg
      121 aagagctggc tcaggcgcgg cttgcaggca ctaaaagcct gtctggaacg ggccgcccag
      181 gccgctaagg cttagaactc gcatatggac tacggccgcc cagccctcgc cgaccgcctg
      241 gccgccgaat acgcgctcgg caccttgcgc ggcggcgcgc gccgccgttt cgagagcctg
      301 ctggccgccc atccgatgct gcgccaggcg gtgcgcgact ggcaggcgcg gctgatgccg
      361 ctcagcggca cggtgcagcc gatacagccc tcgcccgccg tgtggcagcg cgtggagcag
      421 cggctgttcg gcgacggcgt gggcatgacg attgcgtcgc cacggcgttg gtggcaacgc
      481 ctgggcctgt ggcagggcgc tgccggcctg gcagccgtcg cggcgatcgg cctgggcctg
      541 ctgctgtccc aaccgcagcc gacgcagccg cccatcgtga tcgtgatggc cgcgaacccg
      601 caggccggcg ccggagcgat accggcagcc agcttcgtcg ccagcgtcag cgccgacggc
      661 cgcgccctgg tgctcaagcc tatcggggcc atcaccctcg acgccagccg gcaggcgctg
      721 gaactgtggg ccgtgccgag tcatggcgcc ccgcgctcgc tggggctcgt ctcgccgcag
      781 caagcaacga cggtgctgcg cgctcatctg ctgcaggaca ccgccgcctt cgcggtcagc
      841 atggaacccc ccggcggctc gccgaccggg gcgccgaccg ggcccatcct ctcgctgggc
      901 aagttgcgca gctgatctgc atcgcctgca tccagtctcg cgcgaactcc gtacaaggct
      961 gagcgccatc tcagtggcgc ctcaaccctc aacggatgtc cccaggagat tgcgatgaca
     1021 aaccacttct cgactcggct cgcggccccg caccaggacc cgctcgtggc gccgcggcgc
     1081 ggcctgctga aaggcgccgg cctgctgtcg gccggcgcgc tgctgatgct ggccggccag
     1141 cccggccgcg cccagggcat gggcggcagc gaggccgacg tgaacatcct caatgtcgcg
     1201 ctgacgctgg agcacgaggc gatcaacgcc taccagctcg gcgccggcag cggcctgctg
     1261 caaaagcccg tgctcgacgt ggcggtgcgc ttccagtccg accacaagtc gcaccgcgac
     1321 gcgctggtcg cgaccatccg caagctgggc ggcaggccgg tggaggagat gaagctcgag
     1381 caatacgcga agcagctcaa tgcggcgagc ctgaagtcgc aaaccgacgt gctggacctc
     1441 gccgcccgcc tggagctggg cgcgaccaac gcttatctgg gcgtgatccc ctcgttcaag
     1501 gacgcgcagc tggccaaggt ggccggccgc ctggccgccg acgagaccat gcactggacg
     1561 ctgctcaaca acgcgctggg ccgcccgctg ccgggcggcg ccttgagctt cggcgcctga
     1621 cccgcgcagg gcctgccaac gctatagggg cgtggtgctc gcaggcactt cgcccagcgc
     1681 atcgcgcagc gcctcggcga cgcgcgcggg ctgtacttcg gtcaggcagc ggtagtggcc
     1741 gaagcggcag gtgcgctcga agcagggcat gcagtccagc cctagctcgg acttgagcca
     1801 gacgacacgc gcctggctgc tcagcggcgg cgtgtgctcg gggctggtgg agccgaacac
     1861 cgctgcctgc gggatgccga aggcggccgc cacgtgcatc aggcccgagt cgttgctgac
     1921 catgccgcgc gcgccggcga tcagcgccat cgcctcgtcc agcgtcgtgc ggccggccat
     1981 cacatggcag gcgccaggcg ccagcagcga gatctcttcg cagatctcgc cttccttggc
     2041 cgagcccagc agcagcaccg gctggcggct ctgcgcgaag agttggcgcg ccagggccgc
     2101 gtaatgctcg gagggccagc gcttggccgg gccgtactcg gcgcccggcg cgaagaccca
     2161 gtaggcgcgc cggctcacgc cgacgcgctg cagcaccgct tcgacttggg ccggcggcag
     2221 gctcagctga ggcttgagct tgctcgcagt gcggcccgcc agcgcgccgt aataagccgc
     2281 cataggcggg cgctcctggc cgcgcggcgg attggccagg cgctggttga gcagcagcca
     2341 gcggccctcg ccttggtagc ccagccgccg cggaatgcgc gccaaccaag gcaacagcgc
     2401 ggacttgatc gaattgggca gcacataggc acgctcgaag cgcccgcgca gcgtggccgc
     2461 catcgctttg cgcgcggccc agtccagccg gccatgagcg aaaggcagct cgatcacctc
     2521 ggccacctgc ggcatcgcgc gcagcaccgg cgcgacccag ggcagcgccg
//
LOCUS       DASUCY010000270         2747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_246684, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000270 DASUCY010000000
VERSION     DASUCY010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246684"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /gene="fliP"
                     /locus_tag="VFV25_05175"
     CDS             complement(<1..701)
                     /gene="fliP"
                     /locus_tag="VFV25_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701709.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HEY1392742.1"
                     /translation="MSFLSRHRRGLLVATVSLISALPCAALAQQSGGASLPLLIGQGA
                     GGTSYSVPVQTLLFFTALSFLPAVLLMMTGFTRIVIVLSLLRQALGTQSAPPNQVVVG
                     LSLFLTLFVMGPTLDKVYSEAYKPYAANQIGFEEALTRGEAPMRAFMLKQTRQSDVAL
                     FAKLAKLDAAVKPADVPFRVLVPAFVTSELKSAFQIGFLIFIPFLIIDMIVASVLMSL
                     GMMMLSPVLVALPFKL"
     gene            complement(706..1071)
                     /locus_tag="VFV25_05180"
     CDS             complement(706..1071)
                     /locus_tag="VFV25_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HEY1392743.1"
                     /translation="MASTSLSILWFVVIVAMIPLALWLLKRSPYGATLGAAGSGGITR
                     VVSAMPLSPSQRIVTIEVGAGDARQWLVLGVTPQSITTLHQLAPQQPPAAPALNPAAS
                     FASLLHKLQEMRSNDGSQH"
     gene            complement(1094..1525)
                     /gene="fliN"
                     /locus_tag="VFV25_05185"
     CDS             complement(1094..1525)
                     /gene="fliN"
                     /locus_tag="VFV25_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HEY1392744.1"
                     /translation="MSEPNTVAQDAMADEWAAALAQQKDESGRSGELMAEAVAPASFT
                     NFGPGTGTAAGNDINMILDIPVQLTVELGRTRIPIKHILQLAQGSVVELDALAGEPMD
                     VLVNGYLIAQGEVVVVNEKFGIRLTDIVTPSERMRRLSRNA"
     gene            complement(1518..2519)
                     /gene="fliM"
                     /locus_tag="VFV25_05190"
     CDS             complement(1518..2519)
                     /gene="fliM"
                     /locus_tag="VFV25_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HEY1392745.1"
                     /translation="MNQQILSQDEVDALLQGMTGESQKLEPQEQHTGGIREYDLASQE
                     RIVRGRMPTLEIINERFSRNIRIGLFNFIRKTPEVSVGAIKVQKYSAFLREIVVPTNF
                     NIVSVKPLRGSGLVVCDPTLVFAVIDSLFGGQGQFHTRIEGRDFSPTEARIITRLVEV
                     IQTEYRKAWLGVYPLELQYQRSEMQPQFANIAAPSEIVVSTSFSLEIGGTTGSIYFCI
                     PYATLEPIRDVLYSTVQGDYSEPDRRWVKLLKHQIQAAEVAVVAELAQAAATVEQLLA
                     LKPGDFIELDIEKVIEAKVDGVPIFECQYGTSNGRYALKIEQMLTSSDMNWLGEPNV"
BASE COUNT          488 a          873 c          896 g          490 t
ORIGIN      
        1 agcttgaagg gcagggccac cagcaccggc gacagcatca tcatgcccag gctcatcaat
       61 acgctggcga cgatcatgtc gatgatcagg aaggggatga agatcagaaa gccgatctgg
      121 aaggcagact tcagctcgct cgtcacgaag gccggcacca gcacgcgaaa gggcacgtcg
      181 gccggcttca ccgcggcatc gagcttagcc agcttggcga acaaggccac gtcgctctgg
      241 cgcgtctgct tcagcatgaa ggcgcgcatc ggcgcctcgc cgcgcgtcag cgcctcttcg
      301 aagccgatct ggttggcggc ataaggcttg taggcctcgc tgtagacctt gtcgagcgta
      361 ggccccatca cgaacagcgt gaggaagagg ctcaagccga ctaccacctg gttgggcggc
      421 gccgactgcg tgcccagcgc ctggcgcagc aggctcagca cgatgacgat gcgggtgaag
      481 cccgtcatca tcagcagtac cgccggcagg aaggacagcg ccgtgaagaa cagcagcgtc
      541 tgcaccggca ccgaatagct ggtgccgccg gcgccctggc cgatcaggag aggcagcgag
      601 gcgccgccgg actgctgcgc taaagccgcg caagggagcg ccgaaatcag cgatacggtg
      661 gcgaccagca aaccccgccg atggcgcgaa agaaaactca tagcgtcaat gctgcgagcc
      721 atcgttggag cgcatctcct gcagcttgtg cagcaggctc gcgaaggacg ccgccggatt
      781 gagcgcaggc gcggccggcg gctgctgcgg cgcaagctgg tgcagcgtgg tgatgctctg
      841 cggcgtgacg cccagcacca gccattgcct ggcatcgccc gcacccacct cgatggtgac
      901 gatgcgctgg ctgggcgaca gcggcatcgc cgacaccacg cgcgtgatgc cgccgctgcc
      961 ggcagcgccc aaggtcgcgc catagggcga gcgcttgagc agccacaggg cgagaggaat
     1021 catcgcgacg atcacgacga accacaggat cgagaggctg gttgaggcca tgggcgtggt
     1081 ggtccgtgag cgcttatgcg ttgcggctga gccgccgcat gcgctccgaa ggcgtgacga
     1141 tgtcggtcag gcggataccg aacttctcgt tcaccaccac cacctcgccc tgcgcaatca
     1201 ggtagccgtt gaccagtaca tccatcggtt cgccggccag cgcgtcgagc tcgaccaccg
     1261 agccttgcgc gagctgcagg atgtgcttga tcgggatcct cgtgcggccc agctccacgg
     1321 tgagctgcac cgggatgtcg aggatcatgt tgatgtcgtt gccggcggcc gtgcctgtgc
     1381 cgggcccgaa gttggtgaag gacgcgggcg cgacggcttc ggccatcagc tcgccgctcc
     1441 tgccgctctc gtccttctgc tgcgcgagcg cggccgccca ctcgtcggcc atggcgtcct
     1501 gggccaccgt attgggttca gacattgggt tctcctaacc agttcatgtc ggagcttgtg
     1561 agcatctgct cgatcttcag cgcgtacctg ccgttggagg tgccgtactg gcactcgaag
     1621 atgggcacgc cgtcgacctt ggcctcgatc accttctcga tgtcgagctc gatgaagtcg
     1681 ccgggcttca gcgccagcag ctgctcgacc gtcgccgccg cctgcgccag ctcggccacc
     1741 accgcgacct cggcggcctg gatctgatgc ttgagcagct tgacccagcg gcggtccggc
     1801 tcgctgtagt cgccctgcac ggtggaatac agcacgtcgc ggatcggctc cagcgtcgca
     1861 tagggaatgc agaaatagat ggagccggtg gtgccgccga tctccagtga gaaggaggtg
     1921 ctcaccacga tctcgctggg cgcggcgatg ttggcgaact gcggctgcat ctcggagcgc
     1981 tggtattgca gctccagcgg atagacgccc agccaggcct tcctgtattc ggtctggatc
     2041 acctcgacca ggcgcgtgat gatgcgcgcc tcggtgggcg agaagtcgcg gccttcgatg
     2101 cgggtgtgga actggccctg gccgccgaac agcgagtcga tcaccgcgaa caccagcgtc
     2161 gggtcgcaca ccaccaggcc cgagccgcgc agcggcttga ccgacacgat gttgaagttg
     2221 gtcggcacca cgatctcgcg caggaaggcg ctgtacttct gcaccttgat ggcgcccacc
     2281 gagacctcgg gcgtcttgcg gatgaagttg aacaggccga tgcggatgtt gcgcgagaag
     2341 cgctcgttga tgatctccag cgtgggcatg cgcccgcgca cgatgcgctc ctggctcgcc
     2401 aggtcgtact cgcggatgcc gccggtgtgc tgctcctgcg gctcgagctt ctggctctcg
     2461 ccggtcatgc cctgcagcag cgcatcgacc tcgtcttgcg aaaggatctg ctggttcatg
     2521 ttctcagagt gactggtgac ctgtgactag tgacttgtat tgatggctgg cgcggcgtca
     2581 taaacgacca tgtctgatca ctagtcacta gtcaccagtc acaagtcaca gatcactatt
     2641 gaataatgaa gttgatgaag tgcacatgcc gcaccgggtt gtgcacggcc ttcttcttct
     2701 ttttcttctt cgacgcgtct tcgccggcca gcgccgcgga agctcct
//
LOCUS       DASUCY010000271         4256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1162464, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000271 DASUCY010000000
VERSION     DASUCY010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162464"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..1650)
                     /locus_tag="VFV25_05195"
     CDS             complement(106..1650)
                     /locus_tag="VFV25_05195"
                     /inference="COORDINATES: protein motif:HMM:NF025080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY1392746.1"
                     /translation="MGDARRKRERAVSTAVGGDEPMVVDTLGGRMHVRWDEGAAATPN
                     GQLVFFAEFLAAAGVFDRWVETCPLAYTSPNAPGKRDVLGTLVLAILAGHKRYAHVTA
                     LRGDAVAAQALGMNKVISEDALRRALQRIDEPSSEAWLRPTLMDSVREALDRPWVLDI
                     DASIKPLYGRQEGAELGYNPHKPGRPSHVLHTYWVGNLRLVLDVQLNPGKQHTSVHAK
                     AGLARLLDELGDRRPALVRGDCGYGNEGILVTLEQRGQPYLLRLRQTKNVQRLVARQF
                     ARLDWSRPDAQGCQVVEDSLKLEGWSAKRRVVIVRQRIRGGLARERRDVSGQLRLALA
                     DMNVLEGGRLWEYAVLVTDVAYPLEALAQLYRDRCDCENGFDELKNQWGLNGFTTQDL
                     ARCQTTARAGALVYNWWSWYCRAAHPGARLEAVTSRPLLLAAVGKAASHAGQTQLYLT
                     PLHGKLQTIKALIVNIRAALQHVKAIAEQLPRVDRWATLVRYICQRIAPSIAPSPPFN
                     ALPATG"
     gene            2152..3537
                     /locus_tag="VFV25_05200"
     CDS             2152..3537
                     /locus_tag="VFV25_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1392747.1"
                     /translation="MSTTTTTTTAAASRLGAWLRALRDRFALPRDDLLRDLTYRRLWS
                     SILISSFGGQVTMLALPLTAAVLLHASPTQMGLLTTMEILPFVLLSLPSGVWLDRVRK
                     LPVYIAGELSIATVVASVPLAWWMGWLGMPWMYAAAFLMGCIYVTAGSAAQIVLTQVV
                     ARERLVEAHAKNALATSGAEVAGPGLAGALIKLVGAPVALLVDAFLLLISVSILRGIH
                     IVEVRRPKANAHFWHDLAAGVRFVRRSSLLVSLAVVVGLWQMLHNCALVVQILFATRT
                     LGLNEHQVGLSYVGMGLGTITASTLGNRISRRLGPGRCLVLGFAVCGLGWSLLAMAPA
                     NRWGVAAFALMLLCFSAGAVLIFINFLALRQAVTPEPLLGRMTSTMRWLILIPAGPGA
                     LIGGFLGEHVGLRASLSFSGAGALVLALLAWRHPVIRGIKELPTPEPVESLLGAEATV
                     RPAEVVQAEGI"
     gene            3558..4154
                     /locus_tag="VFV25_05205"
     CDS             3558..4154
                     /locus_tag="VFV25_05205"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY1392748.1"
                     /translation="MAMHPDPLLIDVPTRLDTPRLVLRCPQPGDGAAYHEAIVASQDE
                     LRRWMPWAHEPSSEPKYEAYARRAQAEFLSRCDLAMAIFLRQPDGGEGPLIGASGLHR
                     MDWAVRSFEIGYWCHTGYGGQGYISEAVHAITTMAFETLAARRVEIRMDNRNERSRKL
                     AERCGFELEGVLRCDCLDANFELRDTRVYSRVRRDPVL"
BASE COUNT          618 a         1496 c         1409 g          733 t
ORIGIN      
        1 gatctatggc cgcagatatg gcctttgctg atcttcattg ccgtgatgat gggcgtgggc
       61 ctcaaacgct tccggcgtac gctggattga acctaaattc ggcagttacc ccgtagccgg
      121 caaggcgttg aacggtggcg acggggcaat ggacggcgcg atgcgctggc agatgtagcg
      181 tacgagcgtg gcccatcgat cgacgcgcgg caactgctcc gcaattgcct tcacatgctg
      241 caaggccgcg cggatgttga cgatcagcgc cttgatggtc tgcagcttgc cgtgcagcgg
      301 cgtgaggtac agctgggttt gtccggcatg gctggcggcc ttgcccaccg cggccagcag
      361 cagcggtcgg ctggtcaccg cctccaagcg ggccccggga tgcgcggcac ggcagtacca
      421 gctccaccag ttgtagacca gcgccccggc ccgcgccgtg gtctggcagc gggccaggtc
      481 ctgcgtcgtg aagccattga gcccccactg gttcttcagc tcgtcgaacc cgttctcgca
      541 gtcgcagcgg tcccggtaca gctgcgctaa ggcctccaag ggataggcca cgtcggtgac
      601 cagcaccgcg tattcccaca gtcgcccgcc ctcgagcacg ttcatgtccg ccagcgccag
      661 gcgcagttgc ccgctcacgt cccggcgctc ccgtgccagg ccaccccgga tgcgctggcg
      721 cacgatcacc acgcgccgct tggcgctcca gccctccagc ttgagcgagt cctccaccac
      781 ctggcagccc tgggcgtcgg ggcgactcca gtccagccgc gcgaactgcc gcgccaccag
      841 acgctgtacg ttcttcgtct ggcgcagccg cagcagatag ggctgcccgc gctgctccag
      901 cgtcaccagg atgccttcgt tgccgtaacc gcagtcccca cgcaccagtg ccggacgcct
      961 gtcccccagt tcgtcgagca atcgcgccag ccccgccttg gcgtgcaccg aggtgtgctg
     1021 cttgccggga ttgagctgca cgtccagcac caggcgcaga ttgcccaccc agtaggtgtg
     1081 cagcacatga ctgggtcgtc ccggcttgtg ggggttgtag cccagctcgg caccttcttg
     1141 ccgcccgtac agcggcttga tgctcgcgtc gatatccagc acccagggcc gatccagcgc
     1201 ctcgcgcacc gagtccatca gcgttgggcg cagccacgct tcgctactgg gctcgtcgat
     1261 gcgctgcagg gcccggcgca gcgcgtcttc gctgatcacc ttgttcatcc ccagcgcctg
     1321 cgccgccacc gcgtccccgc gcagcgcggt gacatgcgca tagcgcttgt gcccggcaag
     1381 gatcgccagc accagcgtgc ccagcacgtc gcgcttgccc ggcgcattcg gactggtgta
     1441 cgccagcggg caggtctcga cccagcggtc gaacacgccc gcggcggcca ggaactcggc
     1501 gaagaacacc agttgcccgt tgggtgtggc cgccgcgccc tcgtcccagc gaacgtgcat
     1561 gcgcccgccc agcgtgtcca ccaccatcgg ctcgtcgcca cccaccgcgg tcgaaacagc
     1621 ccgctctcgc ttgcgtcgtg cgtcacccat cgggtgagtg tcaccgatcg cccgcagccc
     1681 ccatcagcat tggcgttcag gccgattcgg cgacttcaac tgccgaattt aggctgagtt
     1741 ggctgctaca gcagcgacaa agctgttgcc gccgcgatgc aggcgccgca ttgcgctctt
     1801 gtgggcgagc ggctgcgctg ctagcatggc tcttcaaatc accatcaaac atcagaggag
     1861 ccacacatga acgcgaccac gccgaacagc ctccagtccg ctaaccgcgg cgggctgctc
     1921 gcggcgctgc cgtcgcgcat gcatgtgcga ctgcgctcct cggcagtgcc gtccgtctgg
     1981 cgagcacgaa tcgccgccgg ccctaagcgc tagcggccag ctcgcgcccc tgcggcgcgg
     2041 actcttcctc ctctttccat ttccggttcc ggctgggcgc cacaagcgcc ggccggaacg
     2101 tccaagcatc ctggatgtca ggcgaccgcc ggcatccagc cggagaccat gatgtcgacc
     2161 acgacgacaa caacaacagc agctgcatcc cggctcgggg cctggctccg agccttgcga
     2221 gaccggttcg cgctgccgcg cgacgacctt ttgcgcgacc tgacctaccg ccgtctgtgg
     2281 agctcgatcc tgatcagctc cttcggcggc caggtcacca tgctggcgct gccactgacg
     2341 gccgccgtgc tgctgcacgc cagcccgacg cagatgggcc tcctgaccac gatggagatc
     2401 ctgcccttcg tgctgctctc gctgcccagc ggtgtgtggc tggaccgcgt gcgcaagctg
     2461 ccggtctata tcgcgggcga gctcagcatc gccaccgtgg tggcgagcgt gccgctggcc
     2521 tggtggatgg gctggctcgg catgccctgg atgtatgcag ccgcctttct gatgggctgt
     2581 atctacgtca cggccggttc ggcagcgcag atcgtgctga cgcaggtggt ggcgcgcgag
     2641 cgcctggtgg aggcgcatgc caagaacgcg ctcgccacct cgggcgccga agtggccggc
     2701 cccggcctgg ccggcgcgct gatcaagctc gtcggcgcgc cggtggcgct gctggtcgac
     2761 gcctttctgc tgctgatctc ggtcagcatc ctgcgcggca tccatatcgt tgaggttcgc
     2821 aggcccaagg ccaatgcgca tttctggcac gacctcgcgg ccggcgtgcg tttcgtgcgc
     2881 cgatcgtcgc tgctggtctc gctcgcggtc gtggtcgggc tgtggcagat gctgcacaac
     2941 tgcgcgctgg tggtgcagat cctgttcgct acccgcacgc tggggctcaa tgagcatcag
     3001 gtgggcctga gctatgtggg catgggcctg ggcacgatca cggccagcac cctgggcaac
     3061 cgcatctcgc ggcgcctcgg gccggggcgc tgcctggtgc tgggcttcgc ggtctgcggc
     3121 ctgggctggt cgctgctcgc gatggcaccg gccaaccgct ggggcgttgc cgctttcgcc
     3181 ctgatgttgc tgtgcttcag cgccggcgcg gtgctgatct tcatcaactt cctcgcgctg
     3241 cgccaggccg tgacgcccga gccgctgctg ggccgcatga caagcacgat gcgctggttg
     3301 atcctcatcc ccgccggccc gggcgcgctg atcggtggct tcctcggcga gcatgtgggc
     3361 ttgcgcgcct cgttgagctt ctcgggcgca ggcgccctgg tgctggccct gctggcctgg
     3421 cgccacccgg tgatacgcgg catcaaggaa ctgcccacgc ccgagccggt ggaaagcctg
     3481 ctcggcgccg aggcgaccgt aaggccggcc gaggtggtgc aggccgaagg gatctgagac
     3541 cagggaagag gcgagccatg gcgatgcatc cggacccctt gctgatcgat gtgccgacgc
     3601 ggctggacac gccgcgcctc gtcctgcgct gcccgcagcc gggcgacggc gcggcctatc
     3661 acgaggccat cgtcgcttcg caggacgaac tcaggcgctg gatgccctgg gctcacgagc
     3721 ccagcagcga gcccaagtac gaggcctatg ccaggcgtgc ccaggccgaa ttcctgagcc
     3781 ggtgcgactt ggcgatggcg atcttcctgc gccagcccga tggcggcgaa ggcccgctga
     3841 tcggcgccag cggcctgcac cgcatggact gggccgtgcg cagcttcgag atcggctact
     3901 ggtgccacac gggctatggc ggccagggct acatcagcga ggcggtgcat gcgatcacga
     3961 ccatggcctt cgaaaccctg gccgcgcggc gtgtcgagat ccgcatggac aaccgcaacg
     4021 agcgcagcag gaaactggcc gaacgctgcg gcttcgagct cgaaggcgtg ctgcgctgcg
     4081 actgcctgga cgcgaacttc gagctgcgcg acacaagggt ctattcgcgg gtgaggcgcg
     4141 atcccgtgct gtaagccggc cggccgcggc gatgtctctc acgcggtagc ggcggccaag
     4201 gactgcgtct tctgcaatgc ggccagcgtc tggcggatgc cctcggcgta gggtgt
//
LOCUS       DASUCY010000272         4575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1306683, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000272 DASUCY010000000
VERSION     DASUCY010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306683"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="VFV25_05210"
     CDS             complement(<1..947)
                     /locus_tag="VFV25_05210"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase domain-containing
                     protein"
                     /protein_id="HEY1392749.1"
                     /translation="MKYRDLRGFLQGLEAAGELRRVAEPVSTRLEMTALSDKVLKAAG
                     PALWFQHPVEGGVGYKISALTNLFGTPKRVAMGMGAGDLGELRDLGRMLAALKEPEPP
                     RGLKDAGKLLQMARALWDMKPAKLRSAPCQEVVLEGEAVDLSQLPIQWCWPDDVGPLI
                     TWGLVITRGPQGVPNPRLRQNLGIYRQQVIGRRQVIMRWLAHRGGALDFRDHAQANHG
                     KPFPICVALGADPATILGAVTPVPDSLSEYQFAGLLRGSRTEVVDAGVGDEAGLKLQV
                     PASAEIVLEGHIPAAPPGWRDVSETGVQLKEQGGYLCAL"
     gene            1234..2094
                     /locus_tag="VFV25_05215"
     CDS             1234..2094
                     /locus_tag="VFV25_05215"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY1392750.1"
                     /translation="MMQSLRVFLKDVGNGLLEVSHNSLALVGLVVVAALLFLGSRPDL
                     RSGLEQQALGWLTARQEARVEHNPDMLVALAEPDAVARATAADPAELNRQQAAVALWL
                     SRRYHVAPEPIARLVQEAWGVGQRSSLDPTLILAIMAIESGFNPFAQSAVGAQGLMQV
                     MTRVHDEKYEGFGGAHAAFDPVTNLRVGVQVLKECITRAGSLEGGLRFYVGAANMEDD
                     GGYAGKVLAEQARLRQVAAGRKVPIYTPISTAQSQAASAPAADAQQQAPVESEARPAP
                     QRGEQLALLH"
     regulatory      2121..2240
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01750"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ZMP/ZTP riboswitches appear to regulate genes
                     involved in one-carbon metabolism; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="5-aminoimidazole-4-carboxamide
                     ribonucleotide (ZMP)/
                     5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl
                     5'-triphosphate (ZMT)"
                     /db_xref="RFAM:RF01750"
     gene            2313..3563
                     /gene="glyA"
                     /locus_tag="VFV25_05220"
     CDS             2313..3563
                     /gene="glyA"
                     /locus_tag="VFV25_05220"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEY1392751.1"
                     /translation="MFDRSTSTIANVDPEIWAAIQAENRRQEDHIELIASENYTSPAV
                     MAAQGSQLTNKYAEGYPGKRYYGGCENVDVVEQLAIDRLKQLFGANFANVQANSGSQA
                     NQSVFFGLLQPGDTIMGMSLAEGGHLTHGMALNMSGKWFKVVSYGLNAQEDIDYDAME
                     RLAHEHKPKLIIAGASAFSLRIDFERFARVAKAIGAYFMVDMAHYAGLIAAGVYPNPV
                     PHADVVTSTTHKSLRGPRGGIVLMNSEDIAKKINSAIFPGIQGGPLMHVIAGKAVAFK
                     EALGPEFKVYQQQVVKNAAVLAETLTQRGLRIVSGRTESHVMLVDLRPKGLTGKEAEA
                     ILGQAHMTCNKNGIPNDPQKPMVTSGIRLGSPAFTTRGFKEAEAALTANLIADVLDKP
                     HDEANIAAVCAKVTALTRDFPVYR"
     gene            3609..4052
                     /gene="nrdR"
                     /locus_tag="VFV25_05225"
     CDS             3609..4052
                     /gene="nrdR"
                     /locus_tag="VFV25_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEY1392752.1"
                     /translation="MRCPFCGHEDTQVVETRESDEGDVIRRRRRCPACDKRFTTYERP
                     ELAMPAIVKKDGSRSEFDRNKIRASMLLALRKRPVSIDQVDSALARIEEKLLSSGANE
                     VPSTRIGEMVMRELKKLDKVAYVRFASVYRSFEGVDEFSRLIKDI"
     gene            4135..>4575
                     /locus_tag="VFV25_05230"
     CDS             4135..>4575
                     /locus_tag="VFV25_05230"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family protein"
                     /protein_id="HEY1392753.1"
                     /translation="MMRRVPTSRRPAQAGFTLIELLTVLALLTVLLVLAAPSFVSMRR
                     NSQRVTTANSFVASLDTARTDATKRGISTYVIPLSGQDWTSGWLIYADNDRSQTFTTG
                     DAEIYRQPAMPEGITADNSGGRGNRGFLVGRERYVSFNGSGYPRT"
BASE COUNT          839 a         1609 c         1435 g          692 t
ORIGIN      
        1 tccagcgcac atagatagcc gccctgctcc ttcaactgca cgcccgtctc gctgacgtcg
       61 cgccagccgg gcggcgcggc cgggatatgc ccttccagca cgatctcggc gctggccggc
      121 acctgcagct tgagccccgc ctcgtcgccc acgccggcat cgacgacctc ggtgcggctg
      181 ccacgcagca agccggcgaa ctggtactcg gacaggctgt ccggcaccgg cgtcacggcg
      241 cccaggatgg tggccggatc ggcgccgagc gctacacaaa ttggaaacgg ctttccatga
      301 ttcgcttggg catgatcgcg aaaatccagc gcaccaccgc gatgagccag ccagcgcatg
      361 atgacctgcc gccggcctat cacctgctgg cggtagatgc cgaggttctg ccgcaggcgt
      421 gggttgggta cgccctgcgg cccgcgtgtg atcacgaggc cccaggtgat cagcgggccc
      481 acatcgtccg gccagcacca ttggatgggc agctgggaca agtccaccgc ctcgccctcc
      541 agcacaacct cctggcaagg cgcggaacgc agcttggccg gcttcatgtc ccacagcgcg
      601 cgggccatct gcagcagctt gccggcatcc ttcaggccgc gcggcggctc gggctccttg
      661 agggcggcga gcatgcggcc gaggtcgcgc agctcgccca gatcgcccgc ccccatgccc
      721 atcgcgaccc gcttgggcgt gccgaagagg ttggtcaagg ccgaaatttt gtaaccgaca
      781 cccccctcaa ccgggtgctg aaaccacagc gccgggccgg ccgccttcaa caccttgtca
      841 ctgagggccg tcatctcgag ccgcgtggag actggctccg cgactcgtct gagttcgccg
      901 gcggcctcca ggccttgcaa aaacccccgc agatcccgat atttcataac caattagaat
      961 tagaagcgac gttcataggc ttgactggtt attttcagcc tctcagaatc cgcaccgtct
     1021 cgctagggat tacccgaggc cacgatgccc caggggattc ccagccactt cagtagccga
     1081 gccggccccc tgtctcaggc cggttgttgt aacaggcaat tatctctgcc gcccgcctca
     1141 cgcgcctctc cgcgccaggc agacaggcaa gaaaggagag acatgtcatc atcactcagt
     1201 tattcgagtc gctacgcccg caccggcatt gccatgatgc aatcgctgcg ggtgttcctg
     1261 aaagacgtag gcaacggcct gctggaagtc agccacaact cactggctct tgtgggcctg
     1321 gttgtcgtgg ctgcattgct gttcttgggg agccgccccg acctacgctc cgggctggag
     1381 cagcaggccc tgggctggct gaccgcacgc caggaagcgc gcgtcgagca caaccccgac
     1441 atgctggtcg ccttggccga acccgacgcc gtggcacggg ccaccgccgc cgatccggcc
     1501 gagctcaacc gccagcaggc ggccgtggcg ctttggctgt cgcgccgcta tcacgtggcc
     1561 cccgagccga tcgcgcgcct ggtccaggaa gcctggggcg tgggccagcg cagttcgctg
     1621 gatccgaccc tgatcctggc gatcatggca atcgagtcgg gcttcaaccc cttcgcccag
     1681 agcgctgtgg gcgcccaggg cctgatgcag gtcatgacgc gggtgcacga cgagaaatac
     1741 gagggcttcg gcggcgccca cgcggccttc gacccggtga cgaatctacg cgtcggcgtg
     1801 caggtgctga aggaatgcat cacccgcgcc ggcagcctgg aaggcggcct gaggttctat
     1861 gtcggcgcag ccaacatgga agacgacggc ggctatgccg gcaaggtgct ggccgagcag
     1921 gcccgtctgc gccaagtggc agcgggccgc aaggtgccga tctacacgcc gatcagcacc
     1981 gcgcagagcc aggccgccag cgcgccggcc gccgatgcgc agcagcaggc gccggtcgaa
     2041 tccgaagcgc ggccagcccc gcagcgcggc gaacagctgg ccctgctgca ctgaaaaaag
     2101 gcgcggcgtc cgctacactg agcgggctgc gcaactggcg ataagacccc agtcgggttc
     2161 gaggtggtgc accaccgggg agcgcggccg atgcggcccg tcgctcgcgt cgcgttccgc
     2221 cgttcgcctg ggcagccccc ggaccttgcc ttgctcgtgc agagacaaga cgaagtcttc
     2281 gggaccttca ccctcgaaga ggactgcctc aaatgttcga ccgctccacc tccaccattg
     2341 ccaatgtcga ccccgagatc tgggccgcca tccaggccga gaaccgccgc caggaagatc
     2401 acatcgagct gatcgcctcc gagaactaca cctcgccggc ggtgatggcc gcccagggca
     2461 gccagctcac taacaaatac gccgaaggct atcccggcaa gcgctactac ggcggctgcg
     2521 agaacgtcga cgtggtcgag cagctcgcga tcgaccggct gaagcagctc ttcggcgcca
     2581 atttcgccaa tgtgcaggcg aactccggct cgcaggccaa ccagtccgtg ttcttcgggc
     2641 tgctgcagcc cggcgacacc atcatgggca tgagcctggc cgagggcggc cacctcacgc
     2701 acggcatggc gctcaatatg agcggcaagt ggttcaaggt ggtgagctac ggcctcaacg
     2761 cgcaggaaga catcgactac gatgcgatgg agcgcctggc ccacgagcac aagcccaagc
     2821 tcatcatcgc aggtgcctcg gccttctcgc tgcgtatcga cttcgagcgc ttcgcccgcg
     2881 tggccaaggc catcggcgcc tatttcatgg tcgacatggc ccactatgcc ggcctgatcg
     2941 ccgccggcgt ctaccccaat ccggtgccgc atgccgatgt ggtgacctcc accacccaca
     3001 agagcctgcg cggcccgcgc ggcggcatcg tgctgatgaa cagcgaagac atcgccaaga
     3061 agatcaacag cgcgatcttc cccggcatcc agggcggccc gctgatgcac gtgatcgccg
     3121 gcaaggccgt ggccttcaag gaagcgctcg gccccgagtt caaggtctac cagcagcagg
     3181 tcgtgaagaa cgccgccgtg ctggccgaga cgctgacaca gcgcggcctg cgcatcgtca
     3241 gcggccgcac cgagagccat gtgatgctgg tcgacctgcg ccccaagggc ctgaccggaa
     3301 aggaggccga ggccatcctg ggccaggctc atatgacctg caacaagaac ggcatcccca
     3361 acgacccgca gaagcccatg gtgacgagcg gcatccgcct cggcagcccg gccttcacga
     3421 cgcgcggctt caaggaggcc gaagcggcgt tgacggccaa cctgatcgcc gacgtgctgg
     3481 acaagccgca cgacgaggcc aacatcgcgg cggtgtgcgc caaagtcact gcgctgacac
     3541 gcgacttccc cgtctatcgc tgaggagcca gcgaggccgg gtcctgaacg aatccggcct
     3601 catcgaccat gcgatgcccc ttctgcggcc acgaagacac ccaggtcgtc gagacgcgcg
     3661 agtccgacga gggcgacgtc atacgccggc gccggcgctg cccggcctgc gacaagcgct
     3721 tcacgaccta tgagcgcccc gagctcgcga tgccagccat cgtcaagaag gacggcagcc
     3781 gcagcgagtt cgaccgcaac aagatccgcg cctccatgct gctggcgctg cgcaagcggc
     3841 cagtgagcat cgaccaggtc gacagcgcgt tggcccgcat cgaagagaag ctgctgtcca
     3901 gcggcgccaa cgaagtgcca tcgacccgca tcggcgagat ggtgatgcgc gagctcaaga
     3961 agctggacaa ggtcgcctac gtgcgcttcg cctcggtcta ccgcagcttc gagggcgtgg
     4021 acgagttcag ccggctgatc aaggacatct gagcgccgat ccgcaggccg gcacgctcac
     4081 cagcctgggc gccaagggct gcgatgacgc gagccaaccc gggacatgct ggccatgatg
     4141 cgcagagtgc ccacctcgcg tcgcccggcg caagcaggct tcacgctgat cgagttgctc
     4201 acggttctcg ccctgctgac cgttctgctc gtgctcgccg cgccctcctt cgtgagcatg
     4261 cgccgcaatt cccagcgggt caccacggcc aacagctttg tggcctcgct cgacacggca
     4321 cgcaccgatg ccacgaagcg cggcatcagc acctatgtga tccccttgtc gggacaggac
     4381 tggaccagcg gctggctgat ctacgccgac aacgatcgca gccagacctt caccacgggc
     4441 gacgccgaga tctaccgcca gccggccatg cccgagggca tcacggccga caacagcggc
     4501 ggcagaggca atcggggctt tctagtcggc agggagcgct acgtgtcctt caatggctcg
     4561 ggctatccgc gcacc
//
LOCUS       DASUCY010000273         4772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1226538, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000273 DASUCY010000000
VERSION     DASUCY010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1226538"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="VFV25_05235"
     CDS             complement(<1..791)
                     /locus_tag="VFV25_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY1392754.1"
                     /translation="MAQFFIDRPIFAWVIALFILVVGGVAITQLPVAQYPTVAPPSIT
                     ISTAYPGASAQTLEESVLSVIEQEMNGSPGLIYMESVSQADGTGSITLSFEPGTSPDL
                     AQVDVQNRLSRASPRLPSAVTQQGVRVDKARSNFLLFAILSSDNPAYDPIALGDYASR
                     NLLPEIQRVPGVGQAQLFGTERAMRVWIDPAKLTGFRLSAADVNNAVRGQNAQVSAGG
                     IGNLPLQPGQAISATVTVGGQLSTIEQFGNIVLRANADGSSVRLR"
     gene            complement(804..2093)
                     /locus_tag="VFV25_05240"
     CDS             complement(804..2093)
                     /locus_tag="VFV25_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY1392755.1"
                     /translation="MSKVIRSDLHNTVPGASAPSLSSSLRVALIAVAAATALAACGGK
                     DGAAQGGGVPGGGMPPAQVGVVTVKLQPVALQTELPGRVEAVRVAQVRARVSGVVLKR
                     LFVEGSLVKAGQPLFQIDPAPYRAAYDSAEATLAKAQANLAQATTQADRFKPLVAANA
                     ISKQDMDNANAAQKQAEADVAAAKAAVQTARINLDYASVTAPISGRIGRALVTEGALV
                     TQAEGTQLATIQQTDTMYVNFTQSTSEVLRLRKALAAGRVSNAERGAVRVVLDDGTEL
                     KQSGKLLFSDVTVDQSSGQITLRAEVANPDGLLLPGQYVRVRLSQAELPQGVLLPQQA
                     VTRSNQGDSVMVVGADGKVAPRPVKVSTAEGNQWVVLDGLKPGEQVMVDGFQKLRPNA
                     TVQPVPWQAASASAAAAGASGGAGRQGQPQAQRQAQN"
     gene            2240..2893
                     /locus_tag="VFV25_05245"
     CDS             2240..2893
                     /locus_tag="VFV25_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEY1392756.1"
                     /translation="MARKTKEEAQATRHAILDAAEKLFQQHGVSRSSLQDIAAAAGVT
                     RGAIYWHFRDKADLFNAMMERVTLPLEQSLEVLERADQDDPLADIRALTLQALLQIAN
                     DPQTRRVFEIATHKVEYVDEQLGIRDRHLHSRNGCLVHLEHCLATAKHSARIDSVASP
                     RAIALGLHALIDGLIQNWLLDPAGFDLVAVGLQAVDTYLAGLKTRQRVPKRLAVQTL"
     gene            complement(2974..>4772)
                     /locus_tag="VFV25_05250"
     CDS             complement(2974..>4772)
                     /locus_tag="VFV25_05250"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEY1392757.1"
                     /translation="LVTADYAALYRSLGLDGKPAQQALAQMSTLAPPSGIEGLGSNNW
                     VVAGSHSETGMPLLANDPHLKLSAPALWYFARLEAPGLEVAGATMPGLPVIVLGQNAH
                     IAWGYTNTETDVQDLYLERIKPGDAAQYQTPEGWARFDSHEEIIKVKGGADVKITVRS
                     TRHGPVISDAGDVAKGLTGSRGPADKPAYALAMRWTALDPEVGTIEASMAFNQAKSVA
                     EFVAASAQYVAPMQNMVVADDAGHIAEVSAGRVPLRSADNELKGYVPAPGWDARYDWT
                     GFLEPQLTPRELDPPRGWIATANQRIHAPDYPYFLTSEWTLPYRHQRIEQLLAAQPKH
                     SLASLAAIQADVKSLATERLLPYLRAAKAEHPLAAAAQRELAGFDGTMAGDKAAPAIF
                     YAWWRHLGELILADKLGQDLFEAEFNRRGFRTGLEGILERNDAWWCTAKDQAPAADAC
                     ARRSGEALKLALDELQAKLGPDVTAWRWDALHVARAEHRPFSHVKALAGLFELREPVG
                     GDTYTINVSRVNLKADATTGEFYLAEHGPSLRALYDLKDRGASRFMHSSGQSGLPFSP
                     WYREFLKPWGQVGYVPLWGQGMAHQLSLQPAP"
BASE COUNT          723 a         1616 c         1647 g          786 t
ORIGIN      
        1 tcgcgcaggc gcaccgagga gccgtcggca ttggcgcgca gcacgatgtt gccgaactgc
       61 tcgatcgtgc tgagctggcc gcccaccgtc acggtggcgg agatggcctg gcccggctgc
      121 agcggcagat tgccgatgcc gccggcgctg acctgggcgt tctggccgcg gaccgcgttg
      181 ttgacgtcgg ccgccgagag cctgaagccg gtcagcttgg ccggatcgat ccagacgcgc
      241 atcgcgcgct cggtgccgaa tagctgggcc tggcccacgc cgggcacgcg ctggatctcg
      301 ggcagcaggt tgcgcgaggc gtagtcgccc agagcgatcg ggtcataggc cgggttgtcc
      361 gaggacagga tcgcgaacag caggaagttg gagcgcgcct tgtccacgcg cacgccctgc
      421 tgcgtcacgg cggagggcag gcgcggcgag gcgcggctca gccggttctg cacgtccacc
      481 tgggccaggt cggggctggt gcccggctcg aagctcagcg tgatggaacc ggtgccgtcg
      541 gcctggctga ccgactccat gtagatcagt ccaggcgagc cgttcatttc ctgctcgatc
      601 acgctgagca cgctttcctc cagcgtctgg gcggaggcgc cgggataggc ggtcgagatc
      661 gtgatggaag gcggcgcgac ggtcgggtat tgcgcaaccg gcagctgggt gatggcaacg
      721 ccgcccacca ccaggatgaa cagcgcgatc acccacgcga agatgggacg gtcgatgaag
      781 aactgggcca tggcgctggt tcctcagttc tgggcctggc gctgggcctg cggctggccc
      841 tgcctgccgg cgccgccgct cgcccctgcc gcggccgccg aggcggatgc cgcctgccag
      901 ggcacgggct gcaccgtggc attggggcgc agcttctgga agccgtcgac catcacctgc
      961 tcgccgggct tgaggccgtc gagcacgacc cattgattgc cctcggcggt gctgaccttg
     1021 accggccgcg gcgccacctt gccgtcggcg ccgaccacca tcacgctgtc gccctgattg
     1081 ctgcgcgtca ccgcctgctg cggcagcagc acgccctgcg gcagctcggc ctgggacagg
     1141 cgcacgcgca cgtactggcc gggcagcagc aggccatcgg gattggctac ctcggcgcgc
     1201 agcgtgatct ggcccgagct ctggtccacc gtgacatcgg agaacagcag cttgccgctt
     1261 tgcttgagct cggtgccatc gtccagcacc acgcgcacgg cgccgcgctc cgcattcgac
     1321 acgcggccgg cggccagcgc cttgcgcagg cgcagcacct cgctcgtgga ctgcgtgaag
     1381 ttcacgtaca tcgtgtcggt ctgctggatg gtggcgagct gcgtgccctc ggcctgcgtg
     1441 accagcgccc cttcggtcac cagcgcccgg ccgatgcggc ccgagatcgg cgcggtgacg
     1501 ctcgcgtaat cgagattgat gcgcgccgtc tgcaccgcgg ccttggcggc ggccacgtcg
     1561 gcctcggcct gcttctgcgc cgcattggcg ttgtccatgt cctgcttgct gatcgcattg
     1621 gccgccacca gcggcttgaa gcgatcggcc tgcgtcgtgg cctgggccag attggcctgg
     1681 gccttggcga gcgtcgcctc ggcactgtcg taggcggcgc gataaggcgc cggatcgatc
     1741 tggaacagag gctggccggc cttgacgagg ctgccttcga cgaacaggcg cttgagcacc
     1801 acgccgctca cgcgcgcccg aacctgcgcg acgcgcaccg cctcgacgcg gcccggaagt
     1861 tcggtctgca gcgccaccgg ctgcagcttg accgtcacga cgccgacctg ggccggcggc
     1921 atgccgccgc cagggacacc gccgccttgc gcggcgccgt ccttaccgcc gcaggcggcc
     1981 agggcggttg ccgccgcaac ggcgatcagc gcgacgcgaa gggacgatga gagcgagggg
     2041 gcagatgcgc ccggaactgt gttgtgaaga tcgctgcgga tcacttttga catccttgcg
     2101 aagtggtagg gctcttcgcc tatgcccatg cggcgcgcca tgtcggcgcg gctaggggaa
     2161 aggctggagt ttggagcagc cggcagtata catacaatga tgtatgtttg tgggaaggct
     2221 cagccttctg gagtaccgca tggcacgcaa aacgaaagag gaagcccagg ccacccgcca
     2281 cgccatcctc gacgctgctg aaaagctgtt tcagcagcat ggtgtttcgc gcagctcgct
     2341 gcaggacatc gctgccgcgg ccggcgtgac gcgcggcgcg atctactggc acttcaggga
     2401 caaggccgac ctcttcaacg cgatgatgga gcgcgtcacc ttgccgctgg agcaatcgct
     2461 ggaggtgctc gagcgtgcgg accaggacga cccgctggcc gacatccgcg ccctcacgct
     2521 gcaggccctg ctgcagatcg ccaacgatcc gcagacgcgc cgcgtgttcg agatcgcgac
     2581 ccacaaggtt gagtatgtcg acgagcagct gggcatacgc gatcgccacc tgcatagccg
     2641 caacggttgc ctggtgcacc tggagcactg cctggcaacg gcgaagcaca gcgcgcggat
     2701 cgacagcgtc gcgtcgccgc gcgccatcgc actgggcctg catgccttga tcgacgggct
     2761 gatccagaac tggctgctcg atcctgccgg cttcgatttg gtcgccgtgg gcctgcaggc
     2821 ggtggatact tatctcgccg gcctgaagac gcggcagcga gttcccaagc gcctggccgt
     2881 gcagaccctc taggacacaa gtcgctgcat gctacatatt tagtatttgt attcaacaaa
     2941 tattcatggg tagcgcggtg attttttgtg aatctagggt gccggctgca gactgagctg
     3001 gtgcgccatg ccctgccccc atagcggcac atagcccacc tgcccccaag gcttcaggaa
     3061 ctcgcgatac cagggcgaga acggcagccc agactgcccg ctggaatgca tgaagcgcga
     3121 ggcgccgcgg tccttcaggt cgtagagcgc gcgcagcgaa ggaccatgct cggccaggta
     3181 gaactcgccg gtcgtcgcat ccgccttcag gttcacgcgc gagacattga tcgtgtaggt
     3241 gtcgccgcct accggctcgc gcagctcgaa gagaccggcc agcgccttca catggctgaa
     3301 gggccggtgc tcggcgcgcg cgacatgcag agcgtcccag cgccaggcag tcacgtcggg
     3361 gcccagcttg gcctgcagct cgtccagcgc gagcttgagc gcctcgccgc tgcgccgcgc
     3421 gcaggcatcg gccgcgggcg cctggtcctt ggccgtgcac caccaggcgt cgttgcgctc
     3481 caggatgcct tccaagccgg tgcggaagcc gcgccggttg aattcggcct cgaagagatc
     3541 ctgccccagc ttgtcggcca ggatcagttc ccccaggtga cgccaccagg cgtagaagat
     3601 ggccggcgcc gccttgtcgc ccgccatggt gccgtcgaag ccggccagct cgcgctgcgc
     3661 tgccgcagcc agcggatgct cggccttggc ggcgcgcaga tacggcagca gccgctcggt
     3721 cgcgagcgat ttgacgtcgg cctggatggc cgcgaggctg gccaagctgt gcttgggctg
     3781 cgcggccagc agctgctcga tgcgctgatg gcgataaggc agcgtccatt cgctggtcag
     3841 gaaataaggg tagtccggcg catggatgcg ctggttcgcg gtggcgatcc agccgcgcgg
     3901 cggatcgagc tcgcgcggcg tgagctgggg ctccaggaag ccggtccagt cgtagcgcgc
     3961 gtcccagccc ggtgccggca catagccctt gagctcgttg tccgccgagc gcagcggcac
     4021 gcggccggcc gagacctcgg cgatatgccc cgcatcatcg gccaccacca tgttctgcat
     4081 cggcgcgacg tattgcgcgg aagccgcgac gaactccgcc accgacttcg cctggttgaa
     4141 ggccatcgag gcctcgatgg tgccgacctc gggatcgagc gcggtccagc gcatcgcgag
     4201 cgcataggcg ggcttgtctg cggggcctct ggagccggtc aatcccttgg cgacatcgcc
     4261 ggcgtccgag atcaccgggc cgtggcgcgt gctgcgcacc gtgatcttca cgtccgcgcc
     4321 gcccttgacc ttgatgatct cctcgtggct gtcgaagcgc gcccagcctt cgggcgtctg
     4381 gtattgcgca gcgtcgccag gcttgatgcg ctcgaggtag agatcctgca catcggtctc
     4441 ggtgttggtg tagccccagg caatgtgcgc gttctggccc agcacgatca ccggcaggcc
     4501 gggcatggtc gcgcccgcca cctccaagcc cggcgcttcc agtcgcgcga aataccacag
     4561 cgccggcgcg ctgagcttca gatgcgggtc gttggccagc agcggcatgc cggtctcgct
     4621 gtgcgagccg gccacgaccc agttgttgga gcccaggccc tctatgcccg agggcggcgc
     4681 gagcgtgctc atctgcgcca gggcctgctg cgcaggcttg ccgtccagcc cgaggctgcg
     4741 gtacagcgcc gcgtagtcgg ccgtcaccag cg
//
LOCUS       DASUCY010000274         5540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_102147, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000274 DASUCY010000000
VERSION     DASUCY010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 5540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5540
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102147"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VFV25_05255"
     CDS             <1..433
                     /locus_tag="VFV25_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY1392758.1"
                     /translation="DGRLDLMLANVPSPNGIALSRDGRTLHVAVTRGNAVWRGPLLAN
                     GSISKVGTFRAFFGTSGPDGLAVDAEGRLVVAHASLGGAFVLNPRGEVTHFVRSPTGQ
                     TITNVAYRPDVPELVMTDSEQGIVLTATMPSCGVALFSHAP"
     gene            complement(454..684)
                     /locus_tag="VFV25_05260"
     CDS             complement(454..684)
                     /locus_tag="VFV25_05260"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEY1392759.1"
                     /translation="MKQTRSQKMPTLRVELFEGRTPEQKRALAKELTEACVRVLGGSA
                     DGVDVLFFDIARQDWATGGLLWSDKTQAKAPS"
     gene            complement(688..1437)
                     /locus_tag="VFV25_05265"
     CDS             complement(688..1437)
                     /locus_tag="VFV25_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY1392760.1"
                     /translation="MKPAARSQTEQEVRVDLAAAYRLAALQGWDDTIYTHLSAAVPGE
                     PGVYLINRFGLLFEEVCASNLAKVNLRGEVVDGSGASVNPSGFAIHGAVHAARPDAVC
                     VIHVHSPWAVALSALPEGLLPTSQWAMRLAGRLGRHAYEGLALGADEQARMVAALGQL
                     DALLLEHHGPLVVGRSVAEAFMLMHILEKAAQAQLRAIAAAPGRVGGVDAKTAALTYR
                     QWVGDGEVWDGDLEWPALRRRVERLSPGFRD"
     gene            complement(1438..3027)
                     /locus_tag="VFV25_05270"
     CDS             complement(1438..3027)
                     /locus_tag="VFV25_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HEY1392761.1"
                     /translation="MTATTGAAVHPVDERLPLGRLTALGLQQVLVMYAGAIAVPLIVG
                     RALKLAPEQVALLISADLFCCGLVTLIQSLGMGKYFGIKLPVMMGVTFASVGPMVAMA
                     NVHSGPDGARAIFGAIIGAGVISILIAPLMSKLLRFFPPVVTGTIIAIIGISLMRVGV
                     GWAMGGPANLAQFTDMPALVKMVDKAKLDAAAAAGAAAAMGAAPGASPGTVVPQAVAA
                     AGAAQAAAASPAIKLTAPVPMADNPNYGALDNMGIAAFVLVAILLIVRYGRGFIANIA
                     VLLGIVLGCAVAAATGKMSFDKVVKAQWFDVVTPLAFGVPSFDIVMILTMALVMIVVM
                     IESTGMFLALSEMTGKKLSQSDLSAGLRTDGLGTLLGGLFNTFPYTSFSQNVGLVGVT
                     GVKSRWVCVAAGIIMIVLGMLPKMGALVESVPQFVLGGAGLVMFGMVAATGIRILTGV
                     DFKTNRNNLYIVAIAIGAGMIPLVAPRWSQQMPHSLAPLLESGILLTSIVAVLLNLYF
                     NGYKAADQAAAIEAAKTAEAH"
     gene            complement(3166..4041)
                     /locus_tag="VFV25_05275"
     CDS             complement(3166..4041)
                     /locus_tag="VFV25_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer envelope protein"
                     /protein_id="HEY1392762.1"
                     /translation="MHQNFKRHIVVLAALATCGLSAQAADWSDTSIGYRYGTQFREPV
                     NSNDIKKDIFFLTHVSGYKYGSNFFNVDLLQSDSKDPSGVGSTAGAHEAYIVYRHFLD
                     IGKVSGKDFKAGPMRGWGVTAGFDWNSKTDAGYNSKKRMLVLGPTVEFDVPGFLNVSL
                     LELWESNAPCNNYNAGTAFPVCVSRYSYKAHPMLTAAWGIPVFSLPLSFEGYANFIAS
                     KGHNEFGGSTAAEINIDAQLMYDVGSLWGTPKTFKLGLEYQYWKNKFGNDNGDPFYQG
                     GATARTPMIRAEYHF"
     gene            complement(4107..5312)
                     /locus_tag="VFV25_05280"
     CDS             complement(4107..5312)
                     /locus_tag="VFV25_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HEY1392763.1"
                     /translation="MDAYLLDWANLLLRWAHVITAIAWIGSSFYFVFLDNSLTKPTAA
                     DLVEKGVDGELWAVHGGGFYHPQKYLVAPKQMPEHLHWFYWESYSTWLTGFGLFTVLY
                     LFNAGTFLVDSSVHAWSAGAAIAAALAFLVVFWFVYDVICRAFGQQKNGDLIVGAGVF
                     AFVVLAAWLACQLFAGRAAFLLMGAMLATSMSANVFFWIIPGQRKVVAQLKAGQPVDP
                     IHGWRAKQRSVHNTYFTLPVLVAMLSNHYGWLYQGKSNWLVLVLLMLAGALIRHSFVA
                     RHKALVQGKRVPWEHAIVGTLALVGLAFWLAPAPESAQARAAQAKPVSFAEVKAVIDQ
                     RCVMCHNAQLQNKGVALDQPELIKQHAQMVYQQAAVLKLMPFNNATQITDDERALIKR
                     WYEAGAPAQ"
BASE COUNT          956 a         1830 c         1855 g          899 t
ORIGIN      
        1 cgacgggcga ctggacctca tgctggccaa tgtgccaagc cctaacggca tcgcgctcag
       61 ccgcgacggc cgcaccctgc acgtcgccgt gacgcgcggc aatgcggtct ggcgcgggcc
      121 cttgctggcc aacggctcga tctccaaggt cggcacgttc cgcgccttct tcggcaccag
      181 cggtccggac gggctggcgg tcgatgccga gggccgcctc gtggtcgcgc atgccagcct
      241 agggggcgct ttcgtgctca acccgcgcgg cgaagtgact catttcgtgc gcagtcccac
      301 cggccaaaca atcaccaacg tggcctaccg accggacgtg ccggagctgg tgatgacgga
      361 ttccgaacag ggcatcgtgc tgaccgccac gatgccgagc tgtggcgtgg cgctcttctc
      421 gcatgcgccg taacacaggc tgcggcctcc gactcaacta ggagccttgg cctgggtctt
      481 gtcgctccac aacaggccgc cggtggccca gtcctggcgg gcgatgtcga agaagaggac
      541 atcgacgcca tcggcgctgc cgccaagcac gcgcacgcag gcctcggtga gttccttggc
      601 gagcgcccgc ttctgctcgg gcgtgcggcc ttcgaagagt tcgacgcgca gggtgggcat
      661 cttctggctc cttgtttgct tgattgttca gtcgcgaaat ccgggcgaga gccgctcgac
      721 gcggcgccgc agggccggcc attcgagatc gccgtcccag acctcaccgt cgcccaccca
      781 ttgtcgataa gtcagcgcgg ccgtctttgc atcgacgccc cctacccgcc cgggtgccgc
      841 ggcaatggct cgcagctgcg cctgggccgc cttctccagg atgtgcatca gcataaaggc
      901 ctcggccacg ctgcggccca cgaccagcgg gccatggtgc tccagcagca gcgcgtcgag
      961 ctggccgagc gcggccacca tgcgggcctg ctcgtcggcg cccagcgcca ggccctcgta
     1021 ggcatggcgg ccgaggcggc ccgccaggcg catcgcccat tgcgaagtcg gcagcaagcc
     1081 ctcgggcagg gccgacagcg ccaccgccca ggggctgtgc acgtggatga cgcagaccgc
     1141 gtccggcctg gccgcgtgta ccgcgccgtg gatggcgaag ccgctcgggt tcacgcttgc
     1201 acccgagccg tccacgacct cgccgcgcag gttcaccttc gccagattgg aggcgcagac
     1261 ctcctcgaac agcaggccga agcggttgat caggtagacc ccgggctcgc ccggcaccgc
     1321 cgccgacaga tgcgtgtaga tcgtgtcgtc ccagccctgc agggccgcga gccggtaggc
     1381 ggcggcgagg tcgacgcgca cctcctgctc ggtttggctg cgggctgcag gcttcatcta
     1441 gtgtgcttca gctgttttag ccgcctcgat cgccgcggcc tgatcggcgg ccttgtagcc
     1501 gttgaagtac aggttcagca gcacggcgac gatggaggtg agcaggatgc ctgactcgag
     1561 cagcggggcc aggctgtgcg gcatctgctg cgaccagcgc ggcgccacca gcgggatcat
     1621 gccggcgccg atcgcgatcg cgacgatgta gaggttgttg cggttggtct tgaagtcgac
     1681 gccggtgagg atgcgtatgc cggtcgccgc gaccatgccg aacatcacga ggccggcccc
     1741 gccgagcacg aactgcggga ccgactccac cagagcgccc atcttgggca gcatgcccag
     1801 cacgatcatg atgatgccgg cggccacgca gacccagcgg ctcttgacgc ccgtgacgcc
     1861 aaccaggccg acgttctgcg agaagctggt gtaggggaag gtgttgaaca ggccaccgag
     1921 cagagtgccc aggccgtcgg tgcgcaggcc ggccgacaga tcactctggc tcagcttctt
     1981 gccggtcatc tccgacagtg cgaggaacat gccggtcgat tcgatcatca cgacgatcat
     2041 caccagcgcc atcgtcagga tcatcacgat gtcgaagctc ggcacgccga aggcaagcgg
     2101 cgtgacgaca tcgaaccact gcgccttgac gaccttgtcg aagctcatct tgcccgtcgc
     2161 cgcagcgacg gcgcagccca ggacgatgcc cagcagcacc gcgatgttgg cgatgaagcc
     2221 gcggccgtag cgcacgatca gcaggatggc caccagcacg aaggcggcga tgcccatgtt
     2281 gtcgagcgcc ccgtagttcg ggttgtcggc catcggcacc ggggcggtca gcttgatcgc
     2341 gggcgaggcc gccgcggcct gcgcggcgcc ggcggcggcc accgcctgcg gcaccaccgt
     2401 tcccggtgaa gcgccgggag cggcgcccat cgcagcagcg gcgccggccg cggcagccgc
     2461 gtcgagcttg gccttgtcga ccatcttgac cagcgccggc atgtcggtga actgcgccag
     2521 gttggccggc ccgcccatgg cccagcccac gcccacgcgc atcaggctga tgccgatgat
     2581 cgcgatgatg gtgccggtga ccaccggcgg gaagaagcgc agcagcttgc tcatcagcgg
     2641 cgcgatcagg atggagatca cgccggcgcc gatgatggcg ccgaagatcg cgcgcgcccc
     2701 atcgggcccg ctgtgcacat tggccatcgc caccatggga ccgacggagg cgaaggtcac
     2761 ccccatcatc accggcagct tgatgccgaa atacttgccc atgcccagcg actggatcag
     2821 cgtgaccagg ccgcagcaga acagatcggc cgagatcagc agggccactt gctcgggcgc
     2881 aagcttgagc gcgcggccga cgatcagggg cacggcgatc gcccccgcat acatcaccag
     2941 cacctgctgc aggcccaggg cagtcagccg ccccaggggc agccgctcgt ccaccggatg
     3001 aacggcggcg ccagtcgtcg ctgtcatgtt gtctctcctc aacgggaaca ttgggtgagt
     3061 gacggagcgg cccgctcatg cggcgggctc gcccgctgcg cccctcggga cacgatcgtc
     3121 atgcaagggc gcttgctgcc gcgggagccc gcggcgggct cgaactcaga agtggtactc
     3181 ggcgcggatc atcggcgtgc gtgcggtggc gccgccttga tagaacgggt cgccgttgtc
     3241 gttgccgaac ttgttcttcc agtactggta ctcgaggccg agcttgaagg tcttgggcgt
     3301 gccccacagg ctgccgacgt cgtacatgag ttgcgcgtcg atgttgatct cggccgcggt
     3361 gctgccgccg aactcgttat gacccttgga ggcgatgaag ttggcatagc cttcgaagga
     3421 cagcggcagc gagaagaccg ggatgcccca ggccgcggtc agcatcgggt gggccttgta
     3481 gctgtagcgc gagacgcaca caggaaaggc ggtgccggcg ttgtagttgt tgcagggggc
     3541 attgctttcc cacagctcca gcaggctgac attgaggaag ccggggacgt cgaattccac
     3601 cgtcgggccg agcacgagca tgcgcttctt ggagttgtag cccgcatcgg tcttgctgtt
     3661 ccagtcgaag ccggcggtca caccccagcc gcgcatcggg ccggccttga agtccttgcc
     3721 cgacaccttg ccgatgtcca ggaaatggcg atagacgatg taggcctcgt gcgcgccggc
     3781 agtggacccg acgccggacg ggtccttgct gtccgactgc agcaggtcga cgttgaagaa
     3841 gttcgagccg tacttgtagc cgctgacatg ggtcagaaag aagatgtcct tcttgatgtc
     3901 gttggagttg accggctcgc ggaactgcgt accgtagcga tagccgatgg aggtgtcgct
     3961 ccagtcggca gcctgggcgc tcagaccgca ggtggcgagt gcagcgagca cgacgatgtg
     4021 gcgcttgaag ttctggtgca tctctggctt cctttgtcgt tggttggtgg gggcaatgaa
     4081 tcctgagggt tgatcggccg tgaacgctac tgcgccggcg cgccggcctc gtaccagcgc
     4141 ttgatcagcg cacgctcgtc atcggtgatc tgggtcgcgt tgttgaacgg catcagcttg
     4201 agcacggccg cctgctggta gaccatctgc gcatgctgct tgatcagctc cggctggtcc
     4261 agcgccacgc ccttgttctg caactgcgcg ttatggcaca tcacgcagcg ctggtcgatc
     4321 acggccttga cctcggcgaa gctgaccggc tttgcctgcg cggccctggc ctgcgccgac
     4381 tcgggagccg gtgccagcca gaacgcaaga ccgaccaggg ccagcgtgcc gacgatggca
     4441 tgctcccagg gcacgcgctt gccctgcacc agcgccttgt ggcgcgccac gaagctgtgg
     4501 cgaatcagcg cgcccgcgag catcagcagc accagcacca gccagttgct cttgccctga
     4561 tacagccagc cgtagtggtt tgacagcatc gccaccagca ccggcagcgt gaaataagtg
     4621 ttgtgcacgc tgcgctgctt ggcgcgccag ccgtggatgg ggtccaccgg ctgcccggcc
     4681 ttgagctggg ccaccacctt gcgctggccg gggatgatcc agaagaacac gttggcgctc
     4741 atcgaagtcg ccagcatcgc gcccataagc aagaaggcgg cgcgcccggc gaacagctgg
     4801 caagccagcc aggccgccaa gacgacgaag gcgaagacgc cggcgccgac gatcaggtcg
     4861 ccgttcttct gctggccgaa ggcgcggcag atcacgtcgt agacgaacca gaacacgacc
     4921 aggaaggcca acgccgcggc gatcgccgcg ccggccgacc aggcgtgcac gctgctgtcg
     4981 accaggaagg tgcccgcgtt gaagagatag agcacggtga agaggccgaa tccggtcagc
     5041 caggtggaat agctctccca gtagaaccag tgcaggtgct cgggcatctg cttgggcgcg
     5101 accaggtatt tctgcgggtg atagaagccg ccgccgtgca cggcccacag ctcgccgtcg
     5161 acgcccttct cgaccaggtc ggccgccgtg ggcttggtca ggctgttgtc gagaaaaacg
     5221 aagtagaacg acgagcctat ccaggcgatc gccgtgatga catgggccca gcgcagcagc
     5281 aggttggccc aatcgaggag gtatgcgtcc atcgcgtcct taagagtgcg gaaagcgcta
     5341 ggcacaagcc ttgcgctttc ccctcaccac agcgggctct gtaaggagtg aacatcgcga
     5401 ccaaccggcg gagccctcgg gccccgccgc ggcgggctcc gcggcgacgt gtgtaaactg
     5461 tatacagtgc tgatccatct tgtatacgta gtaaccccaa gatcaccaaa aattcaagag
     5521 ccgcggtagg tcgaataggc
//
LOCUS       DASUCY010000275         2648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_857116, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000275 DASUCY010000000
VERSION     DASUCY010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857116"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /gene="hppD"
                     /locus_tag="VFV25_05285"
     CDS             <1..816
                     /gene="hppD"
                     /locus_tag="VFV25_05285"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEY1392764.1"
                     /translation="GAWGYAGQAGPGELNIPAIKGIGDSLIYLVDRWRGKNGAAEGAI
                     GNIGFFDVDFEPLPGATLSPAGHGLTYIDHLTHNVHRGRMAEWADFYERLFNFREIRY
                     FDIEGQVTGVKSKAMTSPCGKIRIPINEEGNEKAGQIQEYLDKYHGEGIQHIALGSTD
                     LFKTVDALRAADIKLLDTIDTYYELVDKRIPGHGENLAELKKRKILIDGKAGELLLQI
                     FSENQLGPIFFEFIQRKGDQGFGEGNFKALFESIELDQMRRGVLPAAGAKQSD"
     gene            complement(828..1829)
                     /locus_tag="VFV25_05290"
     CDS             complement(828..1829)
                     /locus_tag="VFV25_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY1392765.1"
                     /translation="MLAALQRRTTISLLLAAAIWAWVFAQTSHPYWALGGALLIVFNY
                     ALVLGCEFLISAFLNRTEPVPRASFAQQLRAWLREVQTAPRVFCWRQPFHEHAVPDQP
                     QGRSGQRGIVFIHGFVCNRALWTPWMKRLRALGQPYVAVSLEPVFGSIDDYVPQIEAA
                     VRRIEAGTGAPPVLVCHSMGGLAARAWLGANAAAGGGDARVTRVITIGTPHHGTWLAR
                     WGRTTNTKQMRRLDRGNAWLQALAASEPASRYARFSCFYGHCDNIVFPASTALLPGAE
                     AHHLSGVSHVHMAFHEEIFREALRWLGREESSGADADAAARKGAAAEEGHPYRTLAD"
     gene            complement(1868..>2648)
                     /locus_tag="VFV25_05295"
     CDS             complement(1868..>2648)
                     /locus_tag="VFV25_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEY1392766.1"
                     /translation="RSDAPDPHERLGLRTFLHPTVVSAANFDQRVEGWAGAPQSIYSD
                     HFLGTQAIDGPIGYKLEAPPLHPVIFASTMAGFGPEQAGLMRGFAHVHALLALMRDGF
                     HEKSPGGRVKLRGDDTPLLDYPLTDFVMEGARRAFLSMAEIQFAAGARQVLPVHEQAP
                     PYKTWAQAKEGIAALEMRPLLTRVVSAHVMGGCGMAADERHGVVRPDGQHWQLDNLSV
                     HDGSIFPTSLGANPQLSIYGIVNRLATGLAKRLSGRDVALA"
BASE COUNT          465 a          860 c          924 g          399 t
ORIGIN      
        1 ggcgcctggg gctatgccgg ccaggccggc ccgggcgagc tgaacatccc ggccatcaag
       61 ggtattggcg attcgctgat ctatctggtg gaccgctggc gcggcaagaa cggcgcggcg
      121 gaaggcgcga tcggcaacat cggcttcttt gatgtcgact tcgagccttt gcctggagcg
      181 acactgagcc cggccggcca tggcctgacc tacatcgacc acctcacgca caacgtgcac
      241 cgcggccgca tggccgagtg ggccgacttc tacgagcggc tgttcaactt ccgcgagatc
      301 cgctatttcg acatcgaggg ccaagtcacc ggcgtgaaga gcaaggccat gaccagcccc
      361 tgcggcaaga tccgcattcc gatcaacgaa gagggcaacg agaaggccgg ccagatccag
      421 gagtacctgg acaagtacca cggcgagggc atccagcaca tcgcgctggg ctcgaccgat
      481 ttgttcaaga cggtggacgc gctgcgcgcc gccgacatca agctgctcga cacgatcgac
      541 acctactacg agctggtcga caagcgcatc cccggccatg gcgagaacct ggccgagctg
      601 aagaagcgca agatcctgat cgacggcaag gccggcgagc tgctgctgca gatcttcagc
      661 gagaaccagc tggggccgat cttcttcgag ttcatccagc gcaagggcga ccagggcttc
      721 ggcgagggca acttcaaggc gctgttcgag agcatcgagc ttgaccagat gcgccgcggc
      781 gtgttgcctg ctgccggcgc caagcagtcc gactgagcgg agcgcaatca gtcggctagc
      841 gtgcgatagg ggtggccttc ttcggccgcc gcgcccttgc gcgccgcagc gtctgcatcc
      901 gcgccgctgc tctcctcacg gcccagccag cggagggctt ctctgaagat ctcttcgtgg
      961 aaggccatgt gcacatggga aacaccgctc aggtgatgcg cctcggcgcc cggcagcaga
     1021 gccgtggagg ccgggaagac gatgttgtcg caatgcccgt agaagcagct gaagcgcgca
     1081 tagcgtgagg cgggctcgct ggccgcgagc gcttgcagcc aggcattgcc gcgatccagc
     1141 cggcgcatct gcttcgtgtt ggtcgtgcgc ccccagcgcg cgagccaggt gccgtggtgc
     1201 ggcgtgccta tcgtgatgac gcgggtcacg cgcgcgtcgc cgccgcccgc tgcggcattg
     1261 gccccgagcc aggcacgcgc cgccaggccg cccatgctgt ggcagaccag caccggcggc
     1321 gctccggtgc cggcctcgat gcggcgcacc gcggcttcga tctggggcac atagtcgtcg
     1381 atggagccga acaccggctc caggctcacg gcgacatagg gctggcccag ggcgcgcagt
     1441 cgcttcatcc agggcgtcca cagcgcgcga ttgcagacga agccgtggat gaagacgatg
     1501 ccgcgctggc cgcttcgccc ctgcggctga tcaggcacgg catgctcatg aaagggctgg
     1561 cgccagcaga agacgcgcgg cgctgtctgc acctcgcgga gccaggcacg cagttgctgt
     1621 gcgaagctgg cgcgcggcac cggctccgtg cggttgagga aggcgctgat gaggaactcg
     1681 caacccagca ccagcgcata gttgaagacg atgagcaagg cgccgcccag ggcccaatag
     1741 ggatggctcg tctgcgcgaa gacccaggcc cagatcgcag cggccagcag caaagagatg
     1801 gtagtgcggc gttgcagggc ggcgagcatg gcctatgtta tgccgtggcc gcgcgccgac
     1861 atgcgggtca ggctaaggcg acatcgcgcc cgctcaagcg tttggccaga ccggtcgcga
     1921 ggcggttgac gatgccgtag atcgagagct gcgggttggc acccaggctg gtcgggaaga
     1981 tcgagccgtc atgcactgag aggttgtcga gctgccaatg ctggccgtcc gggcgtacca
     2041 caccgtggcg ttcgtcggcc gccatgccgc agccgcccat cacatgggcg ctcaccacgc
     2101 gggtcagcag cgggcgcatt tccagcgcag cgatgccttc tttcgcttgg gcccaggtct
     2161 tgtagggtgg tgcctgctca tgcaccggca agacctggcg cgcgccggcg gcgaactgga
     2221 tctcggccat gctgaggaag gcgcggcgcg cgccctccat cacaaagtcg gtgagcgggt
     2281 agtcgagcaa gggggtgtcg tcgccgcgca gcttgacgcg gccgcccggc gatttctcgt
     2341 ggaagccgtc gcgcatcagc gccagcaggg cgtgcacgtg agcgaagccc cgcatcagcc
     2401 cagcctgctc tggcccgaag ccggccatgg tggacgcgaa gatcaccgga tgcagcggcg
     2461 gcgcctcgag cttgtagccg atcgggccgt cgatggcctg cgtgccaagg aagtggtccg
     2521 aatagatcga ttgcggcgca ccagcccagc cctcgacgcg ctggtcaaag ttcgcggccg
     2581 agacgaccgt cggatgcaag aaggtgcgca ggccgaggcg ctcgtgcgga tccggcgcgt
     2641 cggagcgc
//
LOCUS       DASUCY010000276         2453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_889322, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000276 DASUCY010000000
VERSION     DASUCY010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_889322"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="VFV25_05300"
     CDS             <1..521
                     /locus_tag="VFV25_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY1392767.1"
                     /translation="FEEVEDLAWYEQLMRINLWGSIWCTQAALPQLKASKGRIVAVSS
                     LAGLIGVPGRTAYSATKFAMTGFFEALRTEVEPQGVSVTIAYPGVVATEIRRHGFDAK
                     GKPAGKSGLDERGAMSVEECARLIAQGMERRQRDIVMSARGKLGRWLKLISPARVDAM
                     ARRALARDATAR"
     gene            518..1075
                     /locus_tag="VFV25_05305"
     CDS             518..1075
                     /locus_tag="VFV25_05305"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY1392768.1"
                     /translation="MTQGSPHEGLQASPWVQRWSGLVGAGARVLDVACGRGRHTRWFS
                     ARGCKVSAVDLDAQAMAGLRGIAEVLVADIENGPWPYAGREFDLVLVTNYLWRPLLPA
                     LLANVAEGGALVYETFAAGNETVGRPARPDFLLRPGELLDLVRGRLRVLGYEDGYLDA
                     PPRFVQRIAAVRERSAGPVPQRYPL"
     gene            1134..2015
                     /gene="dapA"
                     /locus_tag="VFV25_05310"
     CDS             1134..2015
                     /gene="dapA"
                     /locus_tag="VFV25_05310"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEY1392769.1"
                     /translation="MKPIVGSIVALITPMHEDGSVDYPALRKLIDWHIAEGTDCIGVV
                     GTTGESPTVNVEEHCEIIRISVEQAKGRVPIMAGAGGNSTREAIELSKYAKQVGADCT
                     LQVVPYYNKPTQEGIYQHFKAIAEAVDIPVVLYNVPGRSVADMQHETVLRLAQVPGIV
                     GIKEATGNLDRAAWLIKQAPKGFAIYSGDDQTAIGLMLLGGQGNVSVTANVAPRAMHE
                     LCMAAIAGDAKTAREIHMRLLAVHRHLFTEPNPIPVKWALARMGKCGGAIRLPLTPPT
                     AATQAAMEQAMLEAGLL"
     gene            2087..>2453
                     /locus_tag="VFV25_05315"
     CDS             2087..>2453
                     /locus_tag="VFV25_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392770.1"
                     /translation="MPISMRRPLPTRASAARRLAAASLLRLSPLALAAALAACSSGSN
                     LLSNDKIDYRSVGAKTVALDVPPDLTQLARDTRYQPASGTVNASSFQSGAAAAGAAAP
                     AAGQAVAPRAVGDMHIERDG"
BASE COUNT          406 a          841 c          825 g          381 t
ORIGIN      
        1 tgttcgagga agtcgaggac ctggcctggt atgagcagct gatgcgcatc aatctctggg
       61 gcagcatctg gtgcacgcaa gccgccttgc cgcagctgaa ggccagcaag gggcgcatcg
      121 tcgcggtgtc cagcctggcc ggtctgatcg gcgtaccggg gcgcacggcc tatagcgcca
      181 ccaagttcgc aatgacgggc ttcttcgagg cgctgcgtac cgaggtcgag cctcagggcg
      241 tgagcgtgac catcgcttac cccggcgtcg tcgccaccga gatccgccgc catggcttcg
      301 atgcgaaggg caagccagcg ggcaagagcg gcctcgacga gcgcggcgcg atgagcgtgg
      361 aggaatgcgc gcggctgatc gcccagggca tggagcgccg gcagcgcgac atcgtgatga
      421 gcgcaagagg caaacttggg cgctggctca agctcatcag cccggcgcgc gtcgacgcaa
      481 tggcgcgccg ggcgctcgcc agggacgcca ctgcgcgatg acacagggct cgccgcacga
      541 aggcctgcag gcatcgccct gggtgcagcg ctggtcgggg ctcgtgggtg caggagcccg
      601 ggtgctcgac gtggcctgcg gccgcggccg gcatacgcgc tggttctcgg cgcgcggctg
      661 caaggtgagc gcggtggacc tcgacgcgca ggccatggcc gggctgcgcg ggattgccga
      721 ggtattggtc gccgacatcg agaacggccc gtggccatat gccggccgcg agttcgatct
      781 cgtccttgtc accaactacc tgtggcgccc actgctgccc gcactgctgg ccaatgtggc
      841 ggaaggcggc gcactggtct acgagacctt cgcggccggc aacgagaccg tgggacgccc
      901 cgcgcggccg gacttcctgc tgcgccccgg cgagctgctc gatctggtgc gcggccgctt
      961 gcgcgtgctg ggctacgagg acggttacct cgacgcgccg ccgcgcttcg tgcagcgcat
     1021 cgccgccgtg cgcgagcgct cagccgggcc ggtgccccag cgctaccctt tgtgaagccg
     1081 acggggccgg ccttgcccgg ctgagtagaa tccttcgatt ccgaggaacc ccgatgaaac
     1141 ccatagttgg cagcatcgtc gcgctgatca cgccgatgca tgaagacggc agcgtcgact
     1201 accccgcgct gcgcaagctc atcgactggc acattgccga aggcaccgac tgcatcggcg
     1261 tcgtcggcac cacgggcgaa tcgcccacgg tcaatgtcga ggagcattgc gagatcatcc
     1321 gcatctcggt cgagcaggcc aagggccgcg tgcccatcat ggcaggcgcc ggcggcaact
     1381 ccacgcgcga agcgatcgag ctgagcaaat acgccaagca ggtcggcgcc gactgcacgc
     1441 tgcaggtcgt gccctactac aacaagccga cgcaggaagg catctaccag cacttcaagg
     1501 ccatcgccga ggcggtagac atcccggtgg tgctgtacaa cgtgcccggc cgctcggtgg
     1561 cggacatgca gcatgagacg gtgctgcgcc tggcccaggt gcctggcatc gtcggcatca
     1621 aggaagcgac cggcaacctc gaccgcgcag cctggctgat caagcaggcg cccaagggct
     1681 tcgccatcta ttccggcgac gaccaaaccg cgatcggcct gatgctgctc ggtggccagg
     1741 gcaatgtgag cgtcacggcc aatgtcgcgc ctcgcgcgat gcatgagctg tgcatggcgg
     1801 ccatcgccgg cgatgccaaa acggcacgcg agatccacat gaggctgctg gccgtgcaca
     1861 ggcatctatt caccgagccc aatcccatcc ccgtgaaatg ggcgctggcc cgcatgggca
     1921 agtgcggcgg cgccatccgc ctgcctttga ctccgccgac agccgccacc caggcggcca
     1981 tggaacaagc gatgcttgaa gcgggtctgc tctaactcct ccatcattgc cgcggcgcgc
     2041 ctaagtcggc gcccctggct tccagcaagt tttttgcgac cctgacatgc ccatctccat
     2101 gcgtcgtccc ttgcctacac gggcatctgc tgcccgccga ctcgctgccg cttcgctcct
     2161 gcgtctctcg ccgctggctc ttgcagcagc cctggctgcc tgctcgtcag gctcgaacct
     2221 gctgtccaac gacaagatcg actatcgcag cgtcggggcc aagaccgtgg cactggacgt
     2281 tccgccggac ctgacccagt tggcgcgcga cacgcgctat caaccggcga gcggtacggt
     2341 gaatgccagc agcttccagt ccggggcggc tgccgccgga gctgctgcgc ccgcggctgg
     2401 ccaggctgtg gcgccgcgcg ccgttggcga catgcacatc gagcgggacg gca
//
LOCUS       DASUCY010000277         3165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_391315, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000277 DASUCY010000000
VERSION     DASUCY010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391315"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VFV25_05320"
     CDS             <1..376
                     /locus_tag="VFV25_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392771.1"
                     /translation="AGGRGAGPLVIDKEPIMKRTHCYVLGAGLLAAAVGVQAEPPALE
                     STARAMLAGHVACGSPVAYSGLQKRLLEHAAQGVDQLRNYVYITRGIYQLNMTDIGDW
                     MDDWRQAQAHCTVSLAQDKLKR"
     gene            complement(391..1422)
                     /locus_tag="VFV25_05325"
     CDS             complement(391..1422)
                     /locus_tag="VFV25_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY1392772.1"
                     /translation="MQRRSFLRAAGSAAVALAAPCIHAQALEKPKLTIAVGGKNLLYY
                     LPLTVADSLGYFKAEGLDAQIVDFAGGSQALRAVVGGSADVVSGAFEHTIAMQAKGQR
                     MRAFVLQGRAPQVVLAVSNKTMPQFKSVADLKGKKIGVTAPGSSTNIVANYVLAKAGL
                     KPTDVSFIGVGATNAAIAAIRQGQIDALSHLDPVITILQRSGDIRIVSDTRIVAEADK
                     VFGGPMPAGCLYAPQAFIDKYPNTVQALTNAMVRADKWIQAAGAAEIIRVVPEGFLLN
                     DRAVYVDAFLKSKGALSPDGMIPDQGPATAFRALASVDAELAQAKLDLPAIYTNDFVK
                     KANAKYPKG"
     gene            1560..2078
                     /locus_tag="VFV25_05330"
     CDS             1560..2078
                     /locus_tag="VFV25_05330"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008159132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HEY1392773.1"
                     /translation="MTRLTTLQGDITRLAVDAIVNAANSSLLGGGGVDGAIHRAAGPE
                     LLAECRTLGGCPTGEARITRGYRLPARHVIHTVGPVWRGGAFGEPALLAACYRNSLAL
                     AAAHGCARVAFPCISTGIYGYPAQEAADVAITAANAFLQTPAGAAIEEVQFCCFSAGD
                     LALYLQRLPASS"
     gene            2160..2912
                     /locus_tag="VFV25_05335"
     CDS             2160..2912
                     /locus_tag="VFV25_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY1392774.1"
                     /translation="MKKTLLITGASRGIGAATARLAAERGWAVAVNYLRDATAAQATV
                     FAIRERGGEAEAFQADVADDAQVSGLFAAVDSCFGAGSLRGLVNNAGIGDVVCRVDSM
                     TAQRVQRVFATNVLGSFSCAREALRRMSRAHGGPGGAIVNVSSTAARRSSAGIYMDYA
                     ASKAAIDNFTIGLAEEVAAEGVRVNAVRPGIIDTDIHASVGLADRVREMAPTLPMRRA
                     GSADEVARAIVWLLSDEASYVTGALLDVAGGR"
     gene            complement(2922..>3165)
                     /locus_tag="VFV25_05340"
     CDS             complement(2922..>3165)
                     /locus_tag="VFV25_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEY1392775.1"
                     /translation="AAARGFWVQLGAFRDRNGAENFERRVAAELDWLAPLMAIFHEAP
                     MHRLQAGPYASRDEAASAAQRIRTALQLVPVIVERR"
BASE COUNT          480 a         1066 c         1094 g          525 t
ORIGIN      
        1 ggcgggcggc cgtggggcgg gcccgctggt catcgacaag gagcccatca tgaagcgcac
       61 tcactgctat gtgcttggcg ccggcctgct ggccgctgcc gtcggcgttc aggccgagcc
      121 gcctgccttg gagtccaccg cccgcgcgat gttggcgggc catgtcgcct gcggatcgcc
      181 cgtggcgtac agcggcttgc agaaacggtt gctggagcac gcggcgcagg gcgtcgatca
      241 gctgcgcaat tatgtctaca tcacgcgcgg catctatcag ctcaacatga ccgatatcgg
      301 cgattggatg gacgactggc gccaagccca ggcgcattgc accgtctcgc tggcgcagga
      361 caaactcaag cgctagcccc tggccttctc tcagcccttc ggatacttcg cgttcgcctt
      421 cttcacgaag tcgttggtgt agatcgccgg caggtccagc ttggcctggg ccagttcggc
      481 atcgacgctg gccagagccc ggaaggcggt ggccgggccc tggtccggga tcatgccgtc
      541 gggcgagagc gcgcccttgc tcttgagaaa ggcgtcgaca tagacagccc ggtcgttgag
      601 caggaagccc tcgggcacga ccctgatgat ctcggccgcg cccgccgcct ggatccactt
      661 gtcggcgcgc accatggcat tggtcagcgc ctgcacggtg ttcggatact tgtcgatgaa
      721 ggcctgcggc gcgtagaggc agccggccgg catcgggccg ccgaagacct tgtcggcctc
      781 ggcgacgatg cgtgtgtcgc tcacgatgcg gatgtcgccc gagcgctgca ggattgtgat
      841 gaccgggtcg aggtgcgaga gcgcatcgat ctggccctgg cgtatcgcgg cgatcgcggc
      901 attggtcgcc cccacgccga tgaagctcac gtcggtgggc ttgagccccg ccttggcgag
      961 cacgtagttg gcgacgatgt tggtcgacga gccgggcgcc gtcacgccga tcttcttgcc
     1021 cttgaggtcg gccaccgact tgaattgggg catggtcttg ttgctgacgg ccagcacgac
     1081 ctggggcgca cggccctgca gcacgaaggc acgcatgcgc tggcccttgg cctgcatcgc
     1141 aatcgtgtgt tcgaaggcgc ccgacaccac atcggcactg ccgcccacca cggcacgcag
     1201 cgcctgcgag ccgccggcga agtcgacgat ctgcgcgtcc aggccctcgg ccttgaaata
     1261 gcccagcgag tcggccaccg tcagcggcag gtagtacagc agattcttgc cgcccaccgc
     1321 gatcgtgagc ttgggcttct cgagcgcctg cgcgtgaatg cagggtgcgg ccaaagccac
     1381 ggcggccgaa ccggcggcgc gcaggaagga acggcgttgc atcgatgcgt ctcctggtct
     1441 tgttcgggtg gcactgtaag ccgaggtgcg aaaggcccgc taccgggcca gcccgaagtc
     1501 caccggccta ccatcactct caccgcgcgg gcgcgcatcg tttcatcagg aggtgcctca
     1561 tgacccgact cactaccctg caaggcgaca tcacgcggct ggcggtcgat gccatcgtca
     1621 atgcagcgaa ttcgtcgctg ctgggcggcg gcggggtcga cggcgccatc caccgtgccg
     1681 ccggccccga actcttggcc gaatgccgca cgctgggcgg ctgccccacc ggcgaggcgc
     1741 gcatcacgcg cggctaccgg ctgccggcgc gccatgtcat ccataccgtg gggccggtct
     1801 ggcgcggcgg ggccttcggc gagcccgcgc tgctggccgc ctgctatcgc aacagcctcg
     1861 cactggcggc ggcgcatggc tgcgcgcgcg tggcctttcc ctgcatcagc accggcatct
     1921 atggctaccc cgcccaagag gcggccgacg tggcgatcac agcggcgaac gccttcttgc
     1981 agacaccagc cggcgccgcg atcgaagaag tgcagttctg ctgcttcagc gcgggcgacc
     2041 ttgcgctgta cttgcagcgg ctgcctgcat cgagctgagg cctcggccgc gatcagccga
     2101 tcatctcgtg caagccgctc gtcagcggcc agaatgctgg ccaagacaag gaggcctgca
     2161 tgaagaagac gctgctcatc accggtgcca gccgtggcat aggcgcagct accgcgaggc
     2221 tggcagccga gcgtggctgg gcggtggccg tgaactattt gcgcgatgcc acggcggcac
     2281 aagctaccgt ctttgccatc cgcgagcgcg gtggcgaggc tgaggccttc caggccgatg
     2341 tggctgacga tgcgcaagtg agcgggctct tcgccgcggt cgatagctgc ttcggcgccg
     2401 gtagcctgcg cggcctcgtc aacaacgccg gcatcggcga tgtcgtctgt cgcgtcgatt
     2461 cgatgacggc gcagcgcgtg cagcgcgtct tcgcgaccaa tgtgctgggc agcttctcct
     2521 gcgcccgcga ggcactgcgg cgcatgagcc gagcccatgg aggccccggc ggggccatcg
     2581 tcaacgtctc gtcgactgcc gcgcggcgta gctctgctgg catctacatg gattacgcgg
     2641 cctctaaggc ggccatcgac aacttcacga tcggcttggc agaggaggtg gcggcagagg
     2701 gcgtgcgcgt caacgcggtg cggcccggca tcatcgatac ggacatccac gcctcggtgg
     2761 gcctggcgga tcgcgtgcgc gagatggcgc ctacattgcc gatgcgccgc gcgggcagcg
     2821 ccgacgaggt cgcgcgagcc atcgtctggc tgctgtccga cgaggccagc tatgtcaccg
     2881 gggccttgct cgacgtagcg ggtgggcgct aacggccttc atcagcgccg ctcaacgatc
     2941 accggcacca gctgcagcgc ggtgcgtatg cgctgtgccg cgctggccgc ttcgtcgcgg
     3001 ctcgcatagg ggccggcctg cagacggtgc ataggcgctt cgtggaagat cgccatcagc
     3061 ggggccagcc agtccagttc ggccgcgacg cggcgctcga agttctcggc gccattgcgg
     3121 tcgcggaagg cgcccagctg gacccagaag ccgcgcgccg cggcg
//
LOCUS       DASUCY010000278         2747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1017983, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000278 DASUCY010000000
VERSION     DASUCY010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1017983"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VFV25_05345"
     CDS             <1..528
                     /locus_tag="VFV25_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2817 domain-containing protein"
                     /protein_id="HEY1392776.1"
                     /translation="GLFYGGINPTWSNLALRHVLREQGAGVKHLGWIDLHTGLGPSGH
                     GERIFACRDDAAALRRARAWWGPEVTSIYDGSSTSAPLTGLMWCAVYEEMPEAEYTGI
                     ALEYGTLPIMDVMEALRADQWLENHPEAAPEQAELIKRQIRDAFYTDDDAWKHKVVEQ
                     AHVAAHQALRGLSGA"
     gene            681..1688
                     /locus_tag="VFV25_05350"
     CDS             681..1688
                     /locus_tag="VFV25_05350"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY1392777.1"
                     /translation="MNAAAAIDVLFLIPPQVQLLDLAGPAEAFRLANERLAGLGHPPG
                     FRLRYVGPMAEAASSPGLSLHRTETLPPQLEGPSWILLPASAMAGADGAAMPTLLASE
                     AWQACAAWLQQHLRSAVQRDRGHRLITIGGGALLAAAAGLLDKRRCTTHTNALDALHR
                     LAPQAEVVDNRLFLLDGPLASCAGSSAGLDLAVHLLALHCGDAVAAGVARSLALYLRH
                     GPHEPPPAPLLAHRNHLQPALHRLQDALCADPKADWSLQRMASFSKLTARHLSRLFAE
                     HAGATPLHYLQGIRLDKARLALQRGATIAEAAAEGGFRSDQQLRRAWARLLPGSPGGR
                     H"
     gene            complement(1694..>2747)
                     /gene="pgi"
                     /locus_tag="VFV25_05355"
     CDS             complement(1694..>2747)
                     /gene="pgi"
                     /locus_tag="VFV25_05355"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEY1392778.1"
                     /translation="VLRELNPAETLFIIASKTFTTQETMANAYVAKAWFLSHGGTDTA
                     KHFVATTTNVKAAAEFGIATTFGFWDWVGGRYSLWSAIGLPIALAMGADNFRALLAGA
                     HAMDRHFATTPLEKNLPALLGLIDVWYRNFHGYTSRSVAPYHQGLKRLPAYLQQLEME
                     SNGKCVDLAGAPLPFATSPVVWGEPGTNGQHAYFQMLHQGTDVIPVEFIAVKKPTHDL
                     PDLHAKLLANCLAQSQALMLGKTTAQALAERAPTASRELDPSITAKHRSFPGNRPSTT
                     LLLEQLTPRSLGALIALYEHRVFTSGALWGINSFDQWGVELGKALCNDLLPRLASGDA
                     SGLDASTAGLLARLRA"
BASE COUNT          420 a          962 c          934 g          431 t
ORIGIN      
        1 ggcctgttct acggcggcat caacccgacc tggagcaatc tcgcgctgcg ccatgtgctg
       61 cgcgagcagg gcgccggtgt gaagcacctg ggctggatcg acctgcacac cggcctcggc
      121 cccagcggcc atggcgagcg catcttcgcc tgccgcgacg acgccgcggc gctgcgacgc
      181 gcacgcgcct ggtggggccc cgaggtcaca tcgatctacg acggcagctc gacttcggca
      241 ccgctgaccg gcctgatgtg gtgcgcggtc tacgaggaaa tgcccgaggc cgagtacacc
      301 ggcatcgcgc tggagtacgg caccctgccc atcatggacg tgatggaggc cctgcgcgcc
      361 gaccaatggc tggaaaacca ccccgaggct gctcccgagc aggctgagct gatcaagcgt
      421 cagatccgcg acgccttcta caccgatgac gatgcctgga agcacaaggt ggtcgagcag
      481 gcgcatgtag cggcgcatca ggcgctgcgc ggcctgagcg gggcctagcc gcagtgcatg
      541 tcccgaatca atcaggaatc tgaccagtgt ctgccgatgc cgcacgttta atgcggctgc
      601 cggcatccat agaaatcaca aaatgtcctg attcgctgca cggctatcat ggccctcact
      661 ccgcttcggt gattggggcc atgaacgccg ctgccgcgat cgatgtactc ttcctgatcc
      721 cgccccaggt ccagttgctg gatctcgccg ggcctgccga ggcgttccgc ctggccaatg
      781 agcgcctggc agggctgggc catcctccgg gcttccgcct gcgctacgtc ggcccgatgg
      841 ccgaggccgc cagctcgccc ggcctgagct tgcaccgcac cgaaacgctg ccgccccaac
      901 tggaggggcc tagctggatc ctgctaccgg cctcggccat ggcgggcgcc gacggtgcag
      961 ccatgccgac cctgctcgcc agcgaagcct ggcaggcctg cgccgcctgg ttgcagcaac
     1021 atctgcgctc ggcggtgcag cgcgatcgcg gccaccggct gatcacgata ggcggcggcg
     1081 ccctcctcgc cgcggcggcc ggcctgctcg acaagcggcg ctgcacaacg catacgaacg
     1141 ccctcgatgc cctgcaccgc ctcgcgccgc aagccgaggt ggtggacaac cgcctgttcc
     1201 tgctcgacgg ccctttggcc tcctgcgccg ggagcagcgc cgggctcgat ctggcggtgc
     1261 atctgcttgc gctgcattgc ggagatgccg tcgcggcggg cgtcgcgcgc tctctggcgc
     1321 tttacctgcg tcacggcccg cacgagccgc cgcctgcgcc gctgctcgcg caccgcaacc
     1381 acttgcagcc ggcactgcac cgcctgcagg atgcgctctg cgccgaccca aaagccgact
     1441 ggagcctgca gcgcatggcc tccttcagca agctgacggc gcgtcacctg agccgtttgt
     1501 tcgccgagca tgccggcgcc acgccgctgc actacctgca gggcatccgc ctggacaagg
     1561 ccagactggc cctgcagcgc ggcgcgacca tcgccgaagc ggcagccgaa ggaggcttcc
     1621 gcagcgacca gcaactgcgc cgcgcctggg cccgcctgct gccgggctcg ccgggcggca
     1681 ggcactgacc ggcttaagcg cgtagcctgg ccagcagccc ggccgtcgag gcatcgagcc
     1741 cgctcgcatc tccggaggcc aggcgcggca gcaggtcgtt gcacagcgcc ttgccgagct
     1801 ccacgcccca ctggtcgaag ctgttgatgc cccacagcgc gccgctggtg aaaacgcggt
     1861 gttcatagag cgcgatcagc gctccgagcg agcgcggcgt gagctgctcc agcagcagcg
     1921 tcgtgctggg gcgattgccc ggaaagctgc ggtgcttggc agtgatgctg ggatcgagct
     1981 cgcgcgacgc ggtgggcgcg cgctcggcga gcgcctgcgc cgtcgtcttg cccagcatca
     2041 gcgcctgcga ctgcgcgagg cagttggcca gcaacttggc gtggaggtcg ggcaggtcgt
     2101 gcgtcggctt cttgaccgcg atgaactcga ccgggatcac gtcggtgccc tgatgcagca
     2161 tctggaagta ggcgtgctgg ccgttggtgc ccggctcgcc ccaaacgaca gggctggtcg
     2221 cgaaaggcag cggcgcgccg gcaaggtcga cgcacttgcc gttgctctcc atctcgagct
     2281 gctgcagata ggccggcagc cgcttcaggc cttggtggta gggcgcgacg ctacggctcg
     2341 tgtagccatg gaagttgcgg taccagacgt cgatgaggcc cagcagggcg ggcaggttct
     2401 tctccagcgg cgtggtcgcg aaatgccggt ccatcgcatg cgcgccggcc agcagcgcgc
     2461 ggaagttgtc ggcgcccatg gccagcgcga tcggcaggcc gatggccgac cacagcgagt
     2521 agcgcccgcc cacccagtcc cagaagccga aggtcgtggc gatgccgaac tcggccgcgg
     2581 ccttgacgtt ggtcgtggtg gcaacaaagt gcttggcggt gtcggtgccg ccatggctca
     2641 ggaaccaggc cttcgcgacg tatgcgttgg ccatggtctc ctgcgtcgtg aaggtcttgg
     2701 acgcgatgat gaacagcgtc tcggccgggt tcagctcgcg cagcacc
//
LOCUS       DASUCY010000279         6823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_246930, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000279 DASUCY010000000
VERSION     DASUCY010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 6823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6823
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246930"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VFV25_05360"
     CDS             <1..606
                     /locus_tag="VFV25_05360"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY1392779.1"
                     /translation="SACFWLDGAGYAVQAWGDPREAIVALAQRDRHERACLMLDVRMP
                     QLSGLDVHDALLARGAQLPVIYMSGHADVPLTVQAMRKGAVTLLEKPFDDETLQAALD
                     CAFAANPAAPAIAPPAAEAEPPDPEQAQSQARFRAREASLTPREREVLSYVIQGIYNK
                     NIADRIGLSIKTVELYRARGMAKMQARSVATLTRMMVSQRV"
     gene            611..1357
                     /locus_tag="VFV25_05365"
     CDS             611..1357
                     /locus_tag="VFV25_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392780.1"
                     /translation="MSICFTAATPDGRLRLQCPARARHAADAALALLRCDPLLVALQD
                     WTGLPLDWRWEPAAAPLPAAGPHAWALWRLRDDGLLALPWSLLRGLPAPPASLAALLQ
                     WPALPAVLSISQLRLDGDELSQLEPGGALILPESLLSPWHGLLRAAGEPAEAGIPVAL
                     PSPTAPRLAAGDARSIAPPGAGDYEVRLALPDPVPADRLTGWFDGEALAAGPDAGLWR
                     HAGAVPLAAGRLMPWGDGWALAIETVSTID"
     gene            1368..2021
                     /gene="sctR"
                     /locus_tag="VFV25_05370"
     CDS             1368..2021
                     /gene="sctR"
                     /locus_tag="VFV25_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctR"
                     /protein_id="HEY1392781.1"
                     /translation="MDLTQFTPSSALITVIVLALAPFVAVMVTSFTKLVVVLSLLRNA
                     LGLQQVPPNVVLNGLALVLSIYVMYPVGSAMNARIDGMNNIGNSSKTMLQAADAAKEP
                     LRDFLVKHSRPLEREFFLKTAQKALKPEQASVLTDRDFLVVVPAFTVSELSVAFEIGF
                     LIFLPFIVIDLVISNILMAMGMMMLSPTTVSMPFKLLLFVLIDGWVKLTHGLILTYM"
     gene            2029..2301
                     /gene="sctS"
                     /locus_tag="VFV25_05375"
     CDS             2029..2301
                     /gene="sctS"
                     /locus_tag="VFV25_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctS"
                     /protein_id="HEY1392782.1"
                     /translation="MIQGEVIQLTQQALWFVLLLSAPPILVAAVVGLLIAFVQAATQL
                     QEQTLQYVAKFFAIVVTIFITASMLGSGLYQFGDRVFTEFAGMVRR"
     gene            2314..3111
                     /gene="sctT"
                     /locus_tag="VFV25_05380"
     CDS             2314..3111
                     /gene="sctT"
                     /locus_tag="VFV25_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HEY1392783.1"
                     /translation="MEALAIYGPLSDTALLLGLSITRIAVALLLVPLFTSEVVPPLVR
                     NAMFLAIAVLGLALQPSAGPLLPGGWQLVMLFAKEAFIGGAIGLLFAGVMWAFEAAGQ
                     LVDNKVGATQAQVVDPLSGHQTSLNGALFGRLASWVFMAGGGFMLLIGTLIESFALWP
                     VRTPLPGLAAGGVQIFESELGRIMGLTLIVAAPALVLLYLIEGVLGLVNRFAQQLNVF
                     SLAMSLKAVAATWIIWIQLTTLVQLLEDDLLSRHGVVLQALRHLLAG"
     gene            complement(3167..4531)
                     /locus_tag="VFV25_05385"
     CDS             complement(3167..4531)
                     /locus_tag="VFV25_05385"
                     /inference="COORDINATES: protein motif:HMM:NF025684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HEY1392784.1"
                     /translation="MTKPPRRRPFARSVLSLAIAVLAGCAGPAPHRAELAAPAPAAAM
                     ADGTIGDFSAGLRCMDNLLLDYGARDLTVLVEDVAEPGRKPNADAKEALVAAVSDMSR
                     RSRAIRLLVDSPSPNALRPRYVLRSSTSQGDNQTLAVDLKLLAAQDLTVVPGVASRNQ
                     ATPLETVKFGRSFGLGGDAAQARRALMRLGAIEVFGRLAKLPYWTCLGGSADQPEVAA
                     EVRDWYEAMAARPQEIIEYFQQQLSLRGVYDGPQDGSVNPRFKEAVVRYREALGLSRE
                     PKLSKEFLQAYLGANHRELLARIKPGAPLPASPPPQEPLRLSVAAANDASHFSRGENI
                     QLHVRTNRDAHVYCFLQDENKRVLRFFPNRFQPAARVKADEGLQLPGAMRFEIRLNTR
                     GVTETVACFATDRDVLAELPAALGAAADFAPLPVASLGQVRDAFSRVSGGALAQETLQ
                     MQPR"
     gene            complement(5077..6276)
                     /locus_tag="VFV25_05390"
     CDS             complement(5077..6276)
                     /locus_tag="VFV25_05390"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEY1392785.1"
                     /translation="MISARNDDVPALGADEWTMLVREAVRHGVAPLVQARLERSHDLT
                     PPDNVRRALKACHLRTGLDNLRLLTRLAEVLRQWQAHGIAVIVLKGAWLAQSVYADPS
                     LRSMGDADLLVRRTDLERARELLRASGWQEGPATEWTSPTGGGHQLPVFRLGSACLEL
                     HWAIEDDESPFAIDGDGLWQRAVPAQVGGATALALAPEDLLLHLALHTAYGHGWLQFD
                     NGLRGLVDIAACLKYFEGRLNWDILVERARAWRMHACVWLTLMLVRELLRVCAPQAML
                     DRLAPPHADHTPFDAATELVLGHHYQDLRTHLPVLASAWLTKRWHRLPRSAHWRAHAL
                     PCAEALAKAYPALRSRLLRPLRHPAHWADLLNEVAHLSFDRQARSLAARERRRVRMLR
                     WLEMGIN"
     gene            complement(6315..>6823)
                     /locus_tag="VFV25_05395"
     CDS             complement(6315..>6823)
                     /locus_tag="VFV25_05395"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY1392786.1"
                     /translation="VIFQDYVRYNLTARENIWFGNVDRAPDASRIQAAADYAGAGDLI
                     RDLPNGLDTVLGRWFEEGSELSVGEWQKIALARALLREAQVIVLDEPTSAMDAKAEYE
                     LFERFHALAAGRTAILISHRLSTVKMADRIYVLRGGRIVESGTHDELVGRSGAYARLF
                     AAQASGYR"
BASE COUNT         1037 a         2288 c         2359 g         1139 t
ORIGIN      
        1 tcggcctgct tctggctcga cggtgccggc tatgccgtgc aggcctgggg cgacccgcgg
       61 gaggcaatcg tcgcgctggc gcagcgcgac cggcacgagc gcgcctgcct gatgctggat
      121 gtgcgcatgc cgcagctgtc gggcctggat gtgcacgatg cgctgctggc gcgcggcgcg
      181 cagctgccgg tgatctatat gagcggccat gccgacgtgc cgctgaccgt gcaggccatg
      241 cgcaagggcg ccgtcacgct gctggaaaag cccttcgacg acgagacgct gcaggctgcg
      301 ctggactgtg ccttcgctgc caacccggcg gccccagcca tcgctccccc tgccgccgaa
      361 gccgagccac cggacccaga gcaggcgcaa tcgcaggcac gcttccgcgc gcgcgaggcc
      421 tcgctgacgc cgcgcgagcg ggaggtgctc agctacgtga tccaaggcat ctacaacaag
      481 aacatcgccg accgcatcgg cctgtcgatc aagacggtgg agctgtaccg cgcgcgcggc
      541 atggcgaaga tgcaggcgcg ttcggtggcc acactgacgc gcatgatggt gagccagcgg
      601 gtgtgaggcc atgagcatct gcttcaccgc tgccacgcca gatggaaggc tgcgcctgca
      661 gtgtccggct cgtgcgcgcc atgcagccga cgcggcgctg gcgctgctgc gctgcgaccc
      721 cttgctggtc gcgctccagg attggacggg gctcccgctc gattggcgct gggaaccggc
      781 cgcggcgccg ctgcccgccg ccggccccca tgcctgggct ttgtggcgcc tgagggatga
      841 tggcctgctc gcgctgccct ggtcgctgct gcgcggctta cctgcacccc cagcgtcatt
      901 ggcggcgctg ctgcaatggc cggcgctgcc ggctgtgctg tcgatttcgc agctgcgact
      961 ggacggcgac gaactgtcgc agctggaacc cggcggcgcg ctcatcttgc ccgagtcctt
     1021 gctgtcgcca tggcacggct tgctgcgtgc tgccggagag ccggccgaag ccggtatacc
     1081 ggtcgcgctg ccgtcgccta cggcgccgcg actggcggca ggcgacgcac gcagcatcgc
     1141 gccacccggg gccggcgact acgaggtgcg cctcgcgctg cctgaccctg tgccggccga
     1201 ccgcctcacc ggctggttcg acggcgaagc gctggccgcc gggccggatg ccggcctgtg
     1261 gcgccacgct ggcgcggtcc cgctggccgc tggccgcctc atgccctggg gcgacggctg
     1321 ggcactggcg atcgagaccg ttagcactat cgactaagga tctgaccatg gatctgaccc
     1381 aattcacgcc atcgtcggca ttgatcacgg tgatcgtgct cgcgctcgca cctttcgtcg
     1441 cggtgatggt gacctcgttc accaagctcg ttgtcgtgct gagcctcctg cgcaatgcgc
     1501 tcgggctgca gcaggtgccg cccaatgtcg tgctcaacgg gctggcgctg gtgctgagca
     1561 tctatgtcat gtacccggta ggcagcgcta tgaacgcccg tatcgacggc atgaacaaca
     1621 tcggcaacag cagcaagact atgctgcagg ccgctgacgc cgcgaaggag cccttgcgtg
     1681 acttcctcgt caagcacagc cgtccgctgg agcgcgagtt tttcctgaag acggcgcaga
     1741 aggcgctcaa gcccgagcag gcttcggttc tcacagaccg cgacttcctc gtcgtggtgc
     1801 cggccttcac ggtgagcgag ctgtcggtgg ccttcgagat cggtttcctg atcttcctgc
     1861 ccttcatcgt gatcgacctg gtgatctcca acatcctgat ggcgatgggc atgatgatgc
     1921 tgtcgcccac gacggtatcg atgcccttca agctgctcct cttcgtgctg atcgacggct
     1981 gggtcaagct gacccacggc ttgatcctga cctatatgtg aggccgtcat gatccagggt
     2041 gaagtgatcc agctgacgca gcaggcgctg tggtttgtgc tgctgctgtc ggcgccgcct
     2101 atcttggtgg ccgcggtcgt ggggctgctg atcgccttcg tgcaggccgc cacgcagctg
     2161 caggagcaga cgctgcagta tgtggcgaag ttcttcgcca tcgtggtgac catattcatc
     2221 acagcatcga tgctcggcag cgggctctac cagttcggtg atcgtgtgtt cacagagttc
     2281 gccggcatgg taaggcgcta gggccttcac gccatggaag cgcttgccat ctacggtccg
     2341 ctgtcggaca ccgcgctgct gctggggctt tcgatcacgc gcatcgcggt ggccttactg
     2401 ctggtgccgc tgtttacctc ggaggtggtg ccgccgctgg tgcgcaatgc gatgttcctc
     2461 gccatcgccg tgctcggcct cgcgctgcag ccttcggccg ggcccttgct gcccggcggc
     2521 tggcagctgg tgatgctgtt tgcgaaggaa gccttcatcg gcggcgcgat cggcttgttg
     2581 ttcgccggcg tgatgtgggc cttcgaggcg gcggggcagc tggtcgacaa caaggtgggc
     2641 gcgacgcagg cccaggtggt ggatccgcta tcgggtcacc agacctcgct caacggcgcg
     2701 ctgttcgggc gtctggcgag ctgggtcttc atggccggcg gcggcttcat gctgctgatc
     2761 ggcacgctga tcgaaagctt cgcactgtgg ccggtgcgca cacctctgcc cggcttagcg
     2821 gcaggcggag ttcagatctt cgagagcgag ctcggccgca tcatggggct cacgctgata
     2881 gtggcggccc cggcgctggt gctgctgtac ctgatcgagg gcgtgctcgg cctcgtcaac
     2941 cgcttcgccc agcagctgaa cgtattttcg ctggcgatgt cgctgaaggc ggtggcggcg
     3001 acgtggatca tctggatcca gctcacgacg ctggtgcagc tgcttgagga cgatctgctg
     3061 tcgcgccacg gggtcgtgtt gcaggccctg cgtcatctac tggccggctg atcgccggca
     3121 ccagggacgg gaagcactgg gctgggacgc cgccgggtat cgttagtcac cgtggctgca
     3181 tctggagcgt ttcctgagcc agcgcgccgc cactgacgcg gctgaacgca tcgcgcacct
     3241 gccccagcga tgctaccggc agcggtgcaa agtcggcggc agcgccgagc gcggccggca
     3301 attcggccag tacgtcgcga tcggtcgcga agcaggccac ggtttccgtc acgccgcggg
     3361 tgttcagccg gatctcgaag cgcatcgcgc cgggcagctg caggccctcg tcggccttca
     3421 cgcgcgccgc gggctggaag cggttgggaa agaagcgcag cacgcgcttg ttctcgtcct
     3481 gcaggaagca gtagacatgc gcgtcccggt tggtccgcac atggagctgg atgttttcgc
     3541 cgcggctgaa gtggctggcg tcgttggccg cggccacgct cagcctgagc ggctcttgcg
     3601 ggggcggcga agcaggcagc ggcgcaccag gcttgatgcg cgcgagcagc tcgcgatgat
     3661 tggcaccgag ataagcctgc aggaactcct tcgacagctt gggctcgcgc gacaggccga
     3721 gcgcctcgcg gtagcgcacc actgcctcct tgaaccgcgg gttgacgctg ccgtcctgcg
     3781 ggccgtcgta gacgccgcgc aagctcagct gctgctggaa gtactcgatg atctcctgcg
     3841 ggcgcgcggc catcgcctca taccagtcac gaacctcggc cgctacttcg ggctggtcgg
     3901 cactgccgcc gaggcaggtc cagtagggaa gcttcgccag gcgaccgaag acttcgatgg
     3961 cgccgagccg catcagggcg cggcgcgcct gcgctgcgtc gccgccgagg ccgaagctgc
     4021 ggccgaactt gaccgtttcc aagggcgtcg cctggttgcg cgaggcgacg cccgggacga
     4081 cggtcaggtc ctgggcggcg agcagcttga ggtcgacagc cagggtctgg ttgtcgcctt
     4141 ggctggtcga gctgcgcagg acgtagcggg gcctcagcgc gttcggcgac ggactgtcga
     4201 ccagcaggcg tatcgcccgg ctgcgacgcg acatgtccga caccgccgcg acgagcgctt
     4261 ccttcgcatc cgcattgggc ttgcgaccgg gctcggcgac atcctcgaca agcaccgtca
     4321 agtcgcgagc gccgtagtcc agcagcaagt tgtccatgca gcgcaatccg gcggaaaagt
     4381 cgccgatcgt gccgtctgcc atggcggccg caggagccgg cgcagccaac tcggcgcgat
     4441 gaggcgccgg ccccgcgcag cccgcgagca cagcgatggc cagggacagc acggagcgtg
     4501 caaaggggcg tcggcgggga ggcttcgtca tggcaatgaa cctttcggcc gacgcggcac
     4561 tgctcgcgaa cagcgcgccg gccatggttc agttgctgcg acggccgttt gcgttcaagc
     4621 caggcttcca cccagggtgg gcaggagccg ggcacacgct caaggagcta gcccctctat
     4681 cgctcattcc cagcggacca gcaggcagcc agcgccgcag gcaggccgcc agatcatccg
     4741 acgatatcgg cttggcgacg tagtcgcagg actcgcgtcg gtggcggcta tgcgcgccct
     4801 gctgagggcg gcctgggcga gaaatagttc acgaggccgg caatgcagca caacggagga
     4861 ggacttagcg gccgtaaagc ggccacgggc cgcttctggc cgcaagcgtg aggctcaagc
     4921 cgctccatga agcggcctct cggcctgtcc tgcatggcca atcgcaaggc aacaacgggc
     4981 acaccggccg atcgcctcgc cttgggtcga catcgacggc aagaagctga gacgaatgaa
     5041 tgcctggacc cggttcggcc gaatgagcga atcacctcaa ttgataccca tctccagcca
     5101 gcgcagcatg cgcacgcgac ggcgctcgcg ggccgccagc gagcgtgctt ggcgatcgaa
     5161 cgagagatgt gccacctcgt tcagcaggtc ggcccagtgc gctggatgac gcagcggccg
     5221 cagcagcctc gaacgcaacg ctgggtatgc cttcgcgagg gcctcggcac agggcaaggc
     5281 atgcgcgcgc cagtgggcgc tgcgcggcag gcggtgccag cgcttggtga gccaggcgct
     5341 tgcgagcacc ggcaggtgcg tgcgcagatc ctggtagtga tggcccaaca cgagctcggt
     5401 cgccgcgtcg aacggcgtgt gatcggcgtg ggggggcgct agccggtcca gcatggcctg
     5461 cggcgcgcag acccgcagca gctcgcgcac cagcatcagc gtgagccaca cgcacgcatg
     5521 catgcgccac gcgcgggcgc gctcgacgag gatgtcccag ttgaggcgac cctcgaagta
     5581 ctttaggcat gccgcgatgt cgacgagccc gcgcagcccg ttgtcgaact gcagccatcc
     5641 gtggccgtac gcggtgtgca gcgcgaggtg cagcagtaga tcctccggcg cgagcgccag
     5701 cgcggtcgcg ccacccacct gcgcaggcac ggcgcgctgc cacagcccgt cgccgtcgat
     5761 ggcgaagggg ctctcgtcgt cctcgatcgc ccagtgcagc tcgaggcagg cgctgcccag
     5821 ccggaagacc ggcagctggt gaccgccgcc ggtcggcgac gtccattcgg tcgccgggcc
     5881 ctcctgccat ccgctcgcgc gcagcagctc gcgcgcccgc tcgagatcgg tgcgacgcac
     5941 gagcaaatcg gcgtcgccca tcgagcgcag cgaaggatct gcgtagacag attgcgccag
     6001 ccaggcgccc ttcagcacga tgaccgcgat gccgtgcgcc tgccactgcc ggaggacctc
     6061 ggcgagccgc gtcagcagcc gcaggttgtc caggcccgtg cgcaggtggc atgccttgag
     6121 tgcccggcgg acgttgtccg gtggggtcag gtcatgactc cgttccagcc gcgcttggac
     6181 gagcggcgcg acaccgtgcc tcaccgcctc gcggaccagc atcgtccatt cgtcggcacc
     6241 cagcgccggc acgtcgtcgt tgcgcgccga gatcaaggcc aggagggccg catcaatagc
     6301 gctgagcatc gcattcaccg atagccgctc gcctgcgccg caaagagccg cgcatacgcg
     6361 cccgagcgtc ccaccagctc gtcgtgcgtg ccgctctcga cgatgcgccc gccgcgcagt
     6421 acgtagatgc ggtcggccat cttaaccgtc gacaggcgat ggctgatgag gatcgccgtg
     6481 cgtccggcgg ccagcgcatg gaagcgctcg aacagctcgt actcggcctt cgcgtccatg
     6541 gcgctggtgg gctcgtcgag cacgatgacc tgcgcttcgc gcagcagcgc acgcgccagc
     6601 gcgatcttct gccattcgcc gacgctcagc tccgagcctt cctcgaacca gcggccgagc
     6661 acggtgtcca ggccgttggg caaatcgcgg atgaggtcgc ccgcgccggc atagtcggcg
     6721 gcggcctgga tgcgactcgc gtcgggcgcg cgatcgacgt tgccgaacca gatgttctcg
     6781 cgcgccgtga ggttgtagcg cacgtagtcc tggaagatca cgc
//
LOCUS       DASUCY010000280         2550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_455783, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000280 DASUCY010000000
VERSION     DASUCY010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455783"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1264
                     /locus_tag="VFV25_05400"
     CDS             <1..1264
                     /locus_tag="VFV25_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEY1392787.1"
                     /translation="EGIYLWDSEGHKILDAMSGLWCVNLGYGERALIDAATQQLEELA
                     FYNAFFQTATPPAIELAELLAEVAPAGFEHVFFTNSGSEANDTIVRMVRRYWDLRNQP
                     ERQIIISRRNAYHGSTMAGASLGGMAAMHAQGGLPIPGIEHIEQPHWWQLGREMAHED
                     FGRLAAGWLEQKILELGPERVAAFIGEPVQGAGGVIIPPESYWPEVQRICDRHGILLV
                     SDEVICGFGRTGHWFGCERFGFRPDLMTFAKGVTSGYVPLGGVMVGERVAKVLIEQGG
                     EFEHGYTYSGHPVACAVGIANIRLMQRLGLVEKVRSEAAAHLSKLYESLKDHPLVGNA
                     ETCGLMGAIQLVKDQTKSTPFPAALGVGMVCRGHCFANGLVMRAVGDRMIVAPPLITT
                     PEQIAEMGALIRLCLDKTWTEAKAAGWL"
     gene            1350..2306
                     /locus_tag="VFV25_05405"
     CDS             1350..2306
                     /locus_tag="VFV25_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY1392788.1"
                     /translation="MTTTAPAQATHHRRHPPLLAYGCLAASMALVGSYVGLSKLLVTV
                     FPIFLLAGLRFGIAAVAMLGWLKRGTGEVNLSRHDRILLFWESFLGNFMFSICMLFGV
                     ARTSAVAAGVIMAAIPAVVAVLSRLFLGERITHRVALGVACAVGGIALLALSKPAAAQ
                     ATPTGNGAWLGNALLFGAVVCEASYVVIGKRLAGNVSAKRISALMNLWGLVLVAPLAL
                     WAARSFDFGTVTLPMWGLLLFYAVAASVVTVWLWMTGLKHVPAASAGVFSVMLPVSAA
                     AIGVVFLGESFSAAHALAFGLALAGVVLATLPQRRSAAPPLQ"
     gene            complement(2300..>2550)
                     /locus_tag="VFV25_05410"
     CDS             complement(2300..>2550)
                     /locus_tag="VFV25_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392789.1"
                     /translation="RLPDRAAVEAHMIDYMERLTQQGVSWPHAARHIMGLWNGLAGAR
                     RWRQVWSDHRLKALAPREVSQRAQAQLQTAVELAYSTY"
BASE COUNT          364 a          866 c          871 g          449 t
ORIGIN      
        1 cgagggcatc tacctgtggg acagcgaggg ccacaagatc ctggatgcga tgagcggcct
       61 gtggtgcgtg aacctcggct atggcgagcg cgcgctgatc gacgcggcga cgcaacaact
      121 ggaggagctg gccttctaca acgctttctt ccagaccgcg acgccgccgg ccatcgagct
      181 ggcggaactg ctggcggagg tcgcgccggc gggcttcgag catgtcttct tcaccaactc
      241 gggctccgag gccaacgaca ccattgtgcg catggtgcgg cgctactggg acctgcgaaa
      301 ccagccggag cgccagatca tcatcagccg gcgcaatgcc taccacggct ccacgatggc
      361 cggcgcctcg ctgggcggca tggcggcgat gcatgcccag ggcggcctgc cgattccagg
      421 catcgagcac atcgagcagc ctcattggtg gcagctcggc cgcgagatgg cccacgagga
      481 tttcggcagg ctggcagccg gctggctgga acagaagatc ctggagctcg gccccgaaag
      541 agtcgccgcc ttcatcggcg agccggtgca aggtgcgggc ggcgtgatca tcccgcccga
      601 gagctactgg cccgaagtcc agcgcatctg cgatcgccac ggcatcctgc tcgtcagcga
      661 cgaggtcatc tgcggcttcg gccgcacggg gcactggttc ggctgcgagc gcttcggctt
      721 ccgccccgac ctgatgacgt ttgccaaggg cgtgacctcg ggctatgtgc cgctgggcgg
      781 cgtgatggtc ggcgagcgtg ttgccaaggt tctgatcgag caaggcggcg agttcgagca
      841 cggctacacc tattccggcc atccggtggc ctgcgcggtg ggcatcgcca atatccgctt
      901 gatgcagcgg ctcggcctcg tggaaaaagt gcgcagcgaa gccgcggcgc acttgtccaa
      961 gctgtacgag agcctgaagg accatccgct ggtcggcaac gccgagacct gcggcctgat
     1021 gggcgcgatc cagctcgtca aggaccagac caagagcacg cccttccccg ctgcgttggg
     1081 agtcggcatg gtctgccgcg ggcattgctt cgccaacggc ctcgtgatgc gggccgtggg
     1141 cgatcgcatg atcgtcgcgc cgcccttgat caccacgccc gagcagatcg ccgagatggg
     1201 tgcgctgata cgcctgtgtc tggacaagac ctggactgaa gcaaaggccg ccggttggct
     1261 ctaagtggca tgccacgcgg tgccgacgag cgaacggcaa caggcagcgc tggcatcatg
     1321 cacggctgca cccagcctct cgaatccgca tgacgaccac cgctccggcg caggccacgc
     1381 accaccgccg ccatccgccg ctgctggcct acggctgcct cgccgcctcg atggcgctgg
     1441 tgggcagcta tgtgggcctg tccaagctgc tggtcaccgt gtttccgatc ttcctgctgg
     1501 ccgggctacg cttcggtatc gccgccgtcg cgatgctggg ctggctgaag cgcggcaccg
     1561 gcgaggtcaa cctgtcgagg cacgaccgca tcctgctgtt ctgggagagc ttcctcggca
     1621 acttcatgtt ctcgatctgc atgctgttcg gcgtggcgcg cacctcggcc gtggcggccg
     1681 gcgtgatcat ggcggccata ccggccgttg tggcggtcct ttcgcgccta ttcctgggcg
     1741 agcgcatcac ccatcgcgtg gccctgggcg tcgcctgcgc tgtgggcggc atcgccttgc
     1801 tggccctgag taagccggcg gcggcgcagg cgacgcccac cggcaacggt gcctggctgg
     1861 gtaacgctct gctcttcggc gccgtggtct gcgaagccag ctatgtcgtg atcggcaagc
     1921 ggctggccgg caatgtctcg gccaaacgca tcagcgcctt gatgaacctc tgggggctgg
     1981 tgctggtcgc gccgctcgcg ctctgggcgg cccgcagctt cgacttcggc accgtcacgc
     2041 tgccgatgtg ggggctgctg ctcttctacg ccgtggccgc cagcgttgtt actgtgtggc
     2101 tgtggatgac gggcctcaag catgtgcccg ccgcctcggc cggcgtcttc agcgtgatgc
     2161 tgccggtgag cgccgccgcc atcggtgtcg tcttcctggg cgaaagcttc agcgcggccc
     2221 acgccttggc cttcgggctg gcgctggcgg gcgtggtgtt ggcgaccttg ccgcagcggc
     2281 gttcagccgc cccgcccctt caataggtcg agtaggccag ctcgaccgca gtctgcagct
     2341 gcgcctgcgc ccgctgcgac acctctcgcg gcgccagggc cttgagtctg tggtcgctcc
     2401 atacctggcg ccagcggcgc gccccggcca acccgttcca cagccccatg atgtggcggg
     2461 ccgcatgggg ccagctcacg ccctgctgcg tcaggcgctc catgtagtcg atcatgtggg
     2521 cctccacggc cgcgcggtcc ggcagccgtg
//
LOCUS       DASUCY010000281         2692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_279026, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000281 DASUCY010000000
VERSION     DASUCY010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279026"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VFV25_05415"
     CDS             complement(<1..273)
                     /locus_tag="VFV25_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY1392790.1"
                     /translation="MWFTRVSINNPVFATMVMAALCVLGLFSYSQLGVERLPDITPPN
                     VFVSVAYPGASPGAVETELTRPIELALNGIAGIKKIVSNSFEGRSES"
     gene            complement(331..1533)
                     /locus_tag="VFV25_05420"
     CDS             complement(331..1533)
                     /locus_tag="VFV25_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY1392791.1"
                     /translation="MNKWQQTQEPAQKARRRMWVGGAVGLLVVAGVALVPVLSNGSKG
                     PGPKDGSKVAVPLEFTMAEVVTPLVGPMPQTIEFSGPLVAPRTAIVRAKASGTLLALR
                     VAEGSRVKAGESLGAIDLSDLEARVADRSAAVESAQASLAEAERQHAANVGLASQNFI
                     SSTALQSSQAKLDAARAQLKSAEAQLATSRVGVKEAALLAPISGIVGKRYVVPGEKVS
                     AEQQLISVVDLSVLELAGTVGTYEVSLLKPGQEVQVKVEGQDAPVIGRIDRIAPAAEA
                     GTRAIGVVVVLDNRKEVFRAGQYAQARVQLADATQRMSLPLSAVGQASGQDFVWTIEK
                     DALVRRIVITGRRDEARGRVEVIKGLAPDAQVLGLRFDNLKEGSPARVVARRSAPAAS
                     GAPATAKS"
     gene            complement(1654..>2692)
                     /gene="aroC"
                     /locus_tag="VFV25_05425"
     CDS             complement(1654..>2692)
                     /gene="aroC"
                     /locus_tag="VFV25_05425"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEY1392792.1"
                     /translation="AIGCVIDGCPPGIALSEADIQPELDRRRPGTSRHVTQRNEPDEV
                     AILSGVFEGKTTGTPICLLIRNTDQRSKDYGNLLQTFRPGHADYTYWHKYGIRDPRGG
                     GRSSARLTAPMVAAGAVARKWLKEQYGTQFRGCMSQLGEIEIPFEGWEHVGNNPFFAA
                     NVSRIAELEAYMDALRKAGDSVGARIRVEATNVPVGLGEPLFDKLDADIAHAMMGINA
                     VKGVEIGAGFGCVTQKGTEHGDELTPEGFLSNNAGGVLGGISTGQDIVVSIAVKPTSS
                     IRTPRRSIDLAGQATMVETHGRHDPCVGIRATPIAEAMLALVLMDHALRHRAQCADVR
                     VSTPRIAGPQA"
BASE COUNT          404 a          930 c          929 g          429 t
ORIGIN      
        1 gctctcgctg cgcccttcga agctgttgga gacgatcttc ttgatgccgg cgatgccgtt
       61 gagcgccagc tcgatcggcc gcgtcagctc ggtctcgacc gcgcccggcg aggcgcctgg
      121 ataagccacg ctgacgaaga cattgggcgg cgtgatgtcg ggcaggcgct cgacgcccag
      181 ctggctgtac gaaaagaggc ccagtacgca cagcgccgcc atgaccatgg tggcgaagac
      241 cgggttgttg atggatacgc gggtgaacca catgaggagg ctcccgtggg gatcgatgac
      301 ggaaacaccg gggcaaagcc ccggcgcgat tcaggacttg gcggtggcgg gagcgccgga
      361 tgcagccgga gcggagcgcc gggccacgac ccgtgccggc gagccttcct tcaggttgtc
      421 gaagcgcagg cccagcacct gggcgtcggg cgccaggccc ttgatcacct cgacgcggcc
      481 ccgcgcctca tccctgcggc cggtgatcac gatgcggcgc accagcgcgt ccttctctat
      541 ggtccagacg aagtcctggc cggacgcctg gccgacggcc gacaagggca ggctcatgcg
      601 ctgcgtggcg tcggccagct gcacgcgggc ctgcgcgtac tgacccgcgc ggaagacctc
      661 cttgcggttg tccagcacca ccaccacgcc gatcgcccgc gtgcccgcct cggcggccgg
      721 cgcgatgcgg tcgatgcgac cgatgacggg ggcatcctgg ccctcgactt tgacttgcac
      781 ctcctggccg ggcttcaaca gcgagacttc gtaggtgccg accgtgccgg ccaattccag
      841 taccgacaga tcgacgacgc tgatcagctg ctgctccgca ctgaccttct cgcccggcac
      901 cacgtagcgc ttgccgacga tgcccgagat cggcgccagc agggccgcct ccttcacgcc
      961 cacgcgcgag gtcgcgagct gggcctcagc cgacttgagc tgggcccgcg ctgcatcgag
     1021 cttggcctgc gaagactgca gggcggtcga cgaaatgaag ttctggctgg ccagccccac
     1081 attggcggcg tgctggcgct cggcctcggc caggctcgcc tgggccgact cgaccgccgc
     1141 ggagcggtcg gcgacgcggg cttccagatc ggacaggtcg atggccccca gcgattcgcc
     1201 agccttgacg cggctgcctt cggcgacgcg aagcgcgagc aaagtcccgg acgccttggc
     1261 gcgcacgatc gcggtgcgcg gcgcaaccag ggggccagaa aactcgatgg tctggggcat
     1321 cggcccgacg agcggcgtca ccacttccgc catcgtgaat tcgagcggca cggcgacctt
     1381 ggacccgtcc ttggggcccg gccccttgga gccgttgctc agcaccggca ccagcgccac
     1441 gccagccacg accaggaggc ccaccgctcc ccccacccac atccggcgcc gcgccttctg
     1501 ggccggctcc tgagtttgtt gccatttgtt catgcgaaca gtttaggtgg cgtcaagcga
     1561 ctcgccattc ccccttgcga cggattgcag tttcctaggg gcgaagcgtg gaagccgctg
     1621 acaggcggtg ccggccgcgg gccggccggg gtgtcaggcc tgcgggccgg cgatgcgcgg
     1681 cgtgctgacg cgcacatcgg cgcattgcgc gcgatggcgc agcgcatgat ccatcagcac
     1741 cagggccagc atggcctcgg cgatcggcgt ggcacggatg ccgacgcagg ggtcgtggcg
     1801 gccatgggtc tcgaccatgg tcgcctggcc ggccaggtcg atggagcggc gcggcgtgcg
     1861 tatcgagctg gtcggcttga cggcaatcga cacgacgatg tcctgcccgg tgctgatgcc
     1921 gcccagcacg ccgccggcgt tgttgctcaa gaagccctcg ggcgtcagct catcgccgtg
     1981 ctcggtgcct ttctgggtga cgcagccgaa gccggcaccg atctccacgc ccttgacagc
     2041 attgatgccc atcatcgcat gggcaatgtc ggcatcgagc ttgtcgaaca gcggctcgcc
     2101 cagacccacc ggcacgttcg tcgcctcgac ccggatgcgc gcgccgaccg agtcgcccgc
     2161 cttgcgcaag gcgtccatat aggcctccag ctcggcgatg cgcgagacgt tggccgcgaa
     2221 gaagggattg ttccccacat gctcccagcc ctcgaaggga atctcgattt cgccgagctg
     2281 gctcatgcag ccgcgaaact gcgtgccgta ctgctccttg agccatttgc gcgccaccgc
     2341 gccggccgcc accatcggcg ccgtgagccg cgccgagctg cggccgccgc cacgcggatc
     2401 gcggatgccg tatttgtgcc agtaggtgta gtcggcgtgg cccgggcgga aggtctgcag
     2461 gaggttgcca tagtccttgc tgcgctgatc ggtgttgcgg atcagcaggc agatcggcgt
     2521 tccggtggtc ttgccctcga agacaccgga gaggatcgcc acttcgtcag gctcgttgcg
     2581 ctgggtgacg tggcgcgaag tgccggggcg gcggcggtcc agctcgggct ggatgtcggc
     2641 ctcgctcagg gctatgccgg gcgggcagcc gtcgatcacg cagccgatcg cc
//
LOCUS       DASUCY010000282         2461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_166635, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000282 DASUCY010000000
VERSION     DASUCY010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166635"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="VFV25_05430"
     CDS             complement(<1..732)
                     /locus_tag="VFV25_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEY1392793.1"
                     /translation="MDALLLARLQFAANITFHILFPAINIALGWVLLFFRWRWLRTRD
                     DAWLAAYRFWTKVFALSFAIGVVSGITMSFQFGTNWPGFMERVGNIAGPLLGYEVLTA
                     FFLEATFLGVMLFGHGRVSERVHLIATFLVAFGTTVSAFWILSLNSWMQTPAGYEIVG
                     GEFHARSWLAVIFNPSFPYRLTHMLLASCLTVAFLLAGLSAWQLLRGKANAATPKVLR
                     VGLTTAALLVPVQIFVGDQHGLNTLE"
     gene            complement(746..1303)
                     /locus_tag="VFV25_05435"
     CDS             complement(746..1303)
                     /locus_tag="VFV25_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GbsR/MarR family transcriptional regulator"
                     /protein_id="HEY1392794.1"
                     /translation="MTPLVRSFVAHFGEMGSRWGINRTVGQIYALIFVSPAPLHADDI
                     AEQLEFSRSNVSMGLKELQAWRLVRLKHLPGDRREYFEAPTDAWEIFRTLAEERRRRE
                     IEPTLSMLRNALLEPADNDEDRIAQERMKGMHDLIELMTTWFDDVQRMDAKTLAQLMK
                     MGSKVQKLLEFTGKVKVLPGGKASG"
     gene            1306..1515
                     /locus_tag="VFV25_05440"
     CDS             1306..1515
                     /locus_tag="VFV25_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392795.1"
                     /translation="MDVQSLIWLNPTVISENTEISRRPVDESQVQSALAWEGVPFIAI
                     IRRSGGLDRPSKEAMTAFIAESEHE"
     gene            1508..>2461
                     /gene="coaBC"
                     /locus_tag="VFV25_05445"
     CDS             1508..>2461
                     /gene="coaBC"
                     /locus_tag="VFV25_05445"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEY1392796.1"
                     /translation="MSEEERQDLAGKRLVLGLSGGIACYKAAELLRLFVKAGASVQVV
                     MTEAAQQFITPVTMQALSNQRVITSQWDAAEPNNMAHINLSREADAIVIAPASADLMA
                     QLAQGRAGELLSLMCLARRIDSCPLLLAPAMNREMWAHPATQRNLATLRADGTVILGP
                     ASGDQACGEIGDGRMLEAEEIAEEVIAFFRPKALAGQHVLITAGPTFEPIDPVRGITN
                     RSSGKMGFALARAAREAGAQVTLVAGPVHLPTPRGVARIDVQTAQQMYDAVLPAARHH
                     EVFIATAAVADWRPATLSAQKIKKDGKRVAPSFELTENPDIL"
BASE COUNT          448 a          790 c          826 g          397 t
ORIGIN      
        1 ctccagcgtg ttgagcccat gctggtcacc gacgaagatc tgcaccggca ccagcagcgc
       61 cgccgtcgtg aggccgacgc gcaagacctt gggcgtcgca gcgttcgcct tgccgcgcag
      121 caactgccag gcgctcagcc ctgccagcag gaaggccact gtgaggcagg aggccagcag
      181 catgtgcgtg agccggtagg ggaaggaggg gttgaagatc acagccagcc agctcctggc
      241 gtggaactcg ccgcccacga tttcgtagcc tgcaggcgtc tgcatccagg agttgaggct
      301 caggatccag aaggcgctca cggtggtgcc gaacgcgacc aggaaggtgg cgatcagatg
      361 cacgcgctcg ctgacccggc cgtggccgaa cagcatcacg cccaggaagg tggcctcgag
      421 gaagaaggcc gtcagcacct cgtagcccag cagcggcccg gcgatgttgc ccacgcgctc
      481 catgaagccc ggccagttgg tgccgaactg gaagctcatc gtgatgccgc tgaccacgcc
      541 gatcgcgaag ctcagcgcga acaccttggt ccagaagcga taggcggcga gccaggcgtc
      601 gtcccgcgtc ctgagccagc gccagcggaa gaagagcagc acccagccca gcgcgatgtt
      661 gatggcggga aagaggatgt ggaaagtgat gttggccgcg aattgcaagc gggccagcag
      721 cagggcgtcc atcttttgtt tcctttcagc ctgatgcctt gccgcctggc aagactttca
      781 ccttgccggt gaactccagc agcttctgca ccttggagcc catcttcatc agctgggcca
      841 gcgtcttcgc gtccatgcgc tgcacgtcgt cgaaccaggt cgtcatcagc tcgatcaggt
      901 cgtgcatgcc tttcatgcgc tcctgggcga tgcggtcctc gtcgttgtcg gccggctcca
      961 gcagggcatt gcgcagcatc gacagcgtgg gctcgatctc gcggcggcga cgctcctcgg
     1021 ccagggtgcg gaagatctcc caggcgtcgg tgggtgcctc gaaatattcg cggcggtcgc
     1081 ccggcaggtg cttgagccgc accagccgcc aggcctgcag ctccttgagc cccatgctga
     1141 cgttggagcg cgaaaactcc agctgctcgg cgatgtcgtc tgcatgcaag ggggcgggcg
     1201 aaacgaagat cagcgcgtag atctggccga ccgtgcggtt gatgccccag cggctgccca
     1261 tctcgccgaa atgggcgacg aagctgcgca cgagtggagt catggttgga tgtgcagagt
     1321 ttgatctggc tcaatcctac cgtaatttca gaaaatactg aaatttcaag aagacctgtt
     1381 gacgaaagcc aggtgcaatc agccctcgct tgggagggtg tgcccttcat cgcgataatt
     1441 cggcgctcgg gcggcctcga ccgcccaagc aaggaagcca tgactgcctt catcgccgag
     1501 tccgaacatg agtgaagaag aacgccagga tctggccggc aagcgcctgg tgctcgggct
     1561 gtccggcggc atcgcctgct acaaggcggc cgagctgctg cgcctgttcg tgaaggccgg
     1621 tgcgagcgtg caggtcgtga tgaccgaggc ggcccagcaa ttcatcacgc cggtgacgat
     1681 gcaggcgctg tccaaccagc gcgtcatcac cagccagtgg gatgccgccg agccgaacaa
     1741 tatggcgcat atcaacctct cgcgcgaggc cgatgcgatc gtcatcgcgc cagcgagtgc
     1801 ggatttgatg gcccagctgg cgcaaggccg cgcaggcgaa ctgctgagcc tgatgtgcct
     1861 ggcgcgccgc atcgacagct gcccgctgct gctggccccg gcgatgaacc gcgaaatgtg
     1921 ggctcacccg gcgacccagc gcaatctcgc tacgctgcgt gcagatggca cggtgatcct
     1981 cggcccggcg agcggcgacc aggcctgcgg tgagatcggc gacggccgca tgctcgaagc
     2041 ggaggagatc gccgaggagg tgatcgcttt cttccggccc aaggcgctgg ccggccagca
     2101 tgtcctgatc acggcgggcc cgactttcga gcccatcgac ccggtgcggg gcatcaccaa
     2161 ccgctccagc ggcaagatgg ggttcgcgct ggcccgcgcg gcgcgcgagg ccggagccca
     2221 ggtgaccctg gtggccgggc cggtgcattt gccgacgccg cgcggcgtcg cgcgcatcga
     2281 cgtgcagacg gcccagcaga tgtatgacgc ggtgctgcca gccgcgcggc atcacgaggt
     2341 cttcatcgcc acggctgccg tggccgactg gcggccggca acgctgtcgg cgcagaagat
     2401 caagaaggac ggcaagcggg tcgctcccag cttcgagctg accgagaacc ccgacatcct
     2461 c
//
LOCUS       DASUCY010000283         4329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_439739, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000283 DASUCY010000000
VERSION     DASUCY010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439739"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VFV25_05450"
     CDS             <1..344
                     /locus_tag="VFV25_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY1392797.1"
                     /translation="ERRTQLAGTLSGGERQMLAIGRALMGKPRLLMLDEPSLGLAPLI
                     VKEIFNIIQRLRETGVSILLVEQNARAALEVADYGYVLETGEIALEGPASELAHDPRV
                     IETYLGTQGRA"
     gene            complement(384..593)
                     /locus_tag="VFV25_05455"
     CDS             complement(384..593)
                     /locus_tag="VFV25_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392798.1"
                     /translation="MKALILFIGWCLLLVLCWPLALLALVLMPLVWLLSLPLRLIGIS
                     LEAVFALLKAVLMLPARLLGQRSRS"
     gene            716..2287
                     /locus_tag="VFV25_05460"
     CDS             716..2287
                     /locus_tag="VFV25_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011204133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HEY1392799.1"
                     /translation="MLMRRQAQYHTGLLAVCMAALLSACGGGGGGDSGVPVQGSDGSL
                     SDPAVYSTQPGASLAAGAEAAAVTHHQITLGGTAIAYTATAGHMTASDPASGQAQASF
                     FYVAYTADNQNLGTRPVTFFYNGGPGSASVWLHMGSFGPKRIATGEPSISVPQPFPLV
                     DNTESLLDTTDLIFVDAVGTGYSEAIAPNTNQSFWGVDADAAVFRNFVQRYLQVYGRQ
                     ASPKFLFGESYGTTRSAVLANLLETAGVALKGVVLQSSVLDYNSNCGVTGLATTSCAG
                     YLPSYGAVGAYYMLTNPAQPDLASYMQQMRSFTLTSYAPAVSAYLAQPPVQPSASLIN
                     TLSLDTGASVALWQAHFNLDPGTYRAQLLPGSLIGGYDARVFAPYGSALASEGDPSST
                     LITASFAGSIADYLKNTLLYNNASTYVLLSNAIASWNFSHDGQVLPDTVPDLLGAMLQ
                     NPKLQLLSLNGYHDLVTPYFQTEQDLARLPAANQPQVQTRFYAGGHMTYLDDGSRPLE
                     KSDLRSFYQAALAAQ"
     gene            2299..2700
                     /locus_tag="VFV25_05465"
     CDS             2299..2700
                     /locus_tag="VFV25_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017333517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HEY1392800.1"
                     /translation="MTHFSVRAFAKLLTTAALAGLAAAAQAAPDQEKPLQAPLCDPCV
                     PASVRGKAAVAPAAKQARGAELQAQAEAKLRASFAAADLEHAGSITRAQARAAGLGLV
                     ADNFERIDAAGRSRVSFEDLKRYLRSRGAGF"
     gene            2842..3132
                     /locus_tag="VFV25_05470"
     CDS             2842..3132
                     /locus_tag="VFV25_05470"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY1392801.1"
                     /translation="MIRATVAGRRTLAPEVTDALIAKRQQRTPGADLTPRERELLVLM
                     ARGLNNQEIAAELAIAVPTVKFHITNILAKLQVDSRTEAVLTALKHRIVPAP"
     gene            3290..3487
                     /locus_tag="VFV25_05475"
     CDS             3290..3487
                     /locus_tag="VFV25_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392802.1"
                     /translation="MSSRFFQSDLESTFPSSQLHQRRLGPPSRLPTISRPRPAARWLA
                     WLTIATVFALIVGTLAYWLNA"
     gene            complement(3807..3882)
                     /locus_tag="VFV25_05480"
     tRNA            complement(3807..3882)
                     /locus_tag="VFV25_05480"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3847..3849),aa:Arg,seq:ccg)
     gene            4055..>4329
                     /locus_tag="VFV25_05485"
     CDS             4055..>4329
                     /locus_tag="VFV25_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEY1392803.1"
                     /translation="MSDAHSPQDGHQGPIRTPKQLIIAVVAAFLVPIFAIVLLVNYVS
                     TEKKPSAGSTDAMSEQAVATRIQPVGHVEVKDASDLSALKSGEEVFKA"
BASE COUNT          759 a         1505 c         1402 g          663 t
ORIGIN      
        1 aggagcgccg cacgcagctg gccggcacgc tgtcgggcgg cgaacgccag atgctggcca
       61 tcggccgagc cttgatgggc aagccgcgcc tgctgatgct ggacgaaccc agcctgggcc
      121 tcgcgcccct gatcgtgaaa gagatcttca acatcattca gcgcctgcgc gagacgggcg
      181 tcagcatcct gctggtcgag cagaacgcgc gcgccgcgct ggaggtggcc gactacggct
      241 atgtgctgga aaccggcgag atcgcgctgg aaggcccggc ctcagagctg gcgcatgatc
      301 cccgcgtgat cgagacctat ctgggaaccc agggcagggc ttgagactgg ccgccctcca
      361 attcagcccg gcgccgcagc ggctcaggaa cgcgaacgct gccccagcag ccgcgccggc
      421 agcatcagaa ccgccttcag cagcgcgaac acggcctcga ggctgatgcc aatcaggcgc
      481 aagggcagcg acagcagcca caccagcggc atcagcacca gggcgagcag ggccagcggc
      541 cagcacagga ccagcagcag gcaccagccg atgaagagga tgagcgcttt caaagtaggc
      601 tccaaggtgg gttgcgcgga ttttgcctgt gtagcgaagt gcaagccggc ttggtcgctt
      661 cacagcgcgg cgctatgctg cctgcgcgag cgccgcgcag gcgatgaaga tgaggatgct
      721 gatgcgacgt caagcgcaat atcacaccgg gttgctggcc gtgtgcatgg ccgccctgct
      781 gtcggcctgc ggcggcggag gtggcggcga ctcgggcgtg ccagtccagg gcagcgatgg
      841 cagtctgagc gatcccgcgg tctactcgac ccagcccggg gcctcgctgg cggccggcgc
      901 tgaggccgcc gcggtcacgc accatcagat cacgctgggc ggcaccgcta ttgcctacac
      961 ggccacggcc ggccacatga ccgccagcga tccggcatcg gggcaggccc aggcctcgtt
     1021 cttctacgtg gcctatacgg ccgacaacca gaacctcggc acgcggccgg tgaccttctt
     1081 ctacaacggc ggccccggct cggcgagcgt gtggctgcac atgggctcct tcggccccaa
     1141 gcgcattgcg accggcgagc cgagcatcag cgtgccgcag cccttcccgc tggtcgacaa
     1201 caccgaaagc ctgctcgaca cgaccgatct gatcttcgtc gacgcggtcg gcaccggcta
     1261 ctccgaggcg atcgcgccca acaccaacca gagcttctgg ggcgtggacg cggatgcagc
     1321 ggtgttccgc aacttcgtgc agcgctatct gcaggtgtat gggcgccaag cttcgccgaa
     1381 gtttttgttc ggcgagtcct acggcacgac gcgcagcgcg gtgctcgcga acctgctcga
     1441 aaccgcgggc gtggcgctca agggcgtcgt gctgcaatcc tcggtgctcg actacaacag
     1501 caattgcggc gtgaccggcc tggccaccac gagctgcgcc ggctatctgc cgagctatgg
     1561 cgcggtcggc gcctactaca tgctcacgaa tccggcgcag cccgacctcg cgagctatat
     1621 gcagcagatg cgcagcttca cgctcacgag ttacgcgccg gccgtctcgg cctatctggc
     1681 gcagccgccg gtccagccca gcgccagcct gatcaacacc ttgagcctgg acaccggcgc
     1741 gagcgtggcg ctgtggcagg ctcacttcaa tctcgacccg ggtacctatc gggcccaatt
     1801 gctgccgggc agcctgatcg gcggctatga cgcacgcgtc tttgcgccct acggcagcgc
     1861 gctagcgagc gaaggcgacc cttcgagcac gctgatcacc gcctccttcg ccggcagcat
     1921 cgccgactat ctgaagaaca cgctgctcta caacaatgcc tcgacctatg tgctgctaag
     1981 caatgcgatc gccagctgga atttcagcca cgacggccag gtcctgccgg acacggtgcc
     2041 cgatctgctc ggcgcgatgc tgcagaaccc caagctccaa ctgctctcgc tcaacggcta
     2101 tcacgacctc gtcacgccct acttccagac cgagcaggac ctggcgcgcc tgcccgcagc
     2161 gaaccagccg caggtgcaga cgaggttcta cgccggcggc cacatgacct atctggacga
     2221 cggctcccgg ccgctggaga agagcgatct gcgcagcttc tatcaggccg cgctggcggc
     2281 acagtgagga ggccgcaaat gacgcacttc tctgttcgag ccttcgcgaa gctgctgact
     2341 accgcagcgc tggccggcct cgctgccgcg gcgcaagcgg cgcctgacca ggaaaagcct
     2401 ctgcaggccc cgctgtgcga cccctgcgtg ccggcttcgg tgcgcggcaa ggccgctgtc
     2461 gcaccggcag caaagcaggc ccgtggcgcc gaactgcagg cccaagccga ggccaagctg
     2521 cgtgcgagct tcgcagccgc cgatctcgaa cacgccggca gcatcacgcg cgcacaggca
     2581 cgtgcggccg gcttggggct ggtcgcggac aacttcgagc gcatcgatgc cgccggccgg
     2641 agccgcgtga gctttgagga tctgaagcgc tatctgcgca gtcgcggggc cggcttctag
     2701 gaggaagcac cgctccccat tgcgcctgca tcagctgaga gctcaccacg cgcccgcccg
     2761 gcctccgctc ctgcccagca tcaagcactg cagccattgc gccgaccgtt gaagaacgcg
     2821 tcggcgcacg agcttacgtc gatgattcgc gcgaccgtgg ccggccgccg cacactggcg
     2881 cccgaggtca ccgatgcgct catcgccaag cgccagcagc gcactcccgg cgccgatctg
     2941 acgccgcgcg aacgcgagct gctcgtgctc atggcgcgcg gcctgaacaa ccaggagatc
     3001 gccgccgaac tggcgatcgc cgtgccgacg gtcaagttcc acatcaccaa catcctcgcc
     3061 aagctgcagg tcgacagccg caccgaagcg gtgttgacgg cactcaagca tcgcatcgtg
     3121 ccggcgccct gaccgtgcgg ccgcaggtgc gaacctcgac ctaggtcggg gtcttggccc
     3181 cgaccatagc ccggcgcgcg cgcacgggtc gattcttacg atagctccga aggccagaca
     3241 gcgttctccg ccgccggcgg cgaccacgct aggccagagg agccatcgca tgagttctcg
     3301 gttctttcag tcggacctcg agtccacgtt cccaagcagc cagctccacc agcgacgcct
     3361 ggggccgccg tcgcgtctgc cgaccatcag ccggccgcgg ccggctgcgc ggtggctggc
     3421 ttggctcacc attgcgacgg tgttcgcgct gatcgtcggg accttggcct actggctgaa
     3481 cgcgtgaggc catggagtta cctcgggaag aggacactca ggaggacgaa gccagcccga
     3541 gtcttgcgca gagcgttcct gccggctacg tcgggccgct cgcaatccct ggcaccgggc
     3601 ggctgatctg gtggaccggg cgggtcgcga tcgggctgcg ctacgagccg ccgcttggca
     3661 gccaatcgcg gggacgggaa gaccaacggg aatgaagcta ttcattgcag accgtcgcgg
     3721 ccgcgtgagc ttcgatgcgg gcttctgctt cccaggctcg tcgctcgtcg tgatcatcat
     3781 cgcgaacgac tgcgctgagg gaaggctggc gcgcccggct gggatcgaac cagcaacccc
     3841 tgccttcgga gggcagtact ctatccattg agctacgggc gcctgatgga ggctgggaat
     3901 tctagcagcg gccctgcgca acgccggagc gcgcgagggg cggccgtata atcccgcggt
     3961 tttgcgcgcc tgtgaggctt tcgtctgccg agcgacgcgc cgccgctttc ccaagagagt
     4021 tttcacgagt aaaagagcag cgcccgagga cttcatgagc gacgcacatt ccccccaaga
     4081 cggccaccaa ggcccgatcc ggactcccaa gcagctgatc attgccgtgg tggccgcctt
     4141 cctggtgccc atcttcgcca tcgtgctgct ggtcaactat gtgtcgaccg agaagaagcc
     4201 cagcgccggc agcaccgacg cgatgagcga gcaagccgtg gccacgcgca tccagccggt
     4261 gggccatgtc gaggtcaagg acgcttccga cctcagcgcg ctcaagagcg gcgaagaggt
     4321 gttcaaggc
//
LOCUS       DASUCY010000284         2704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1596239, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000284 DASUCY010000000
VERSION     DASUCY010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1596239"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            318..1256
                     /locus_tag="VFV25_05490"
     CDS             318..1256
                     /locus_tag="VFV25_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEY1392804.1"
                     /translation="MLAFHSDRYTLPLPEGHRFPLAKYRMLRELVAAQLPAVRQQEAP
                     AASDGELVLAHEPDYVAAVAEGWLTPAQQREIGFSWSPQMAERARRSVGATIVAARAA
                     LREGVAANLAGGTHHAHADKGSGYCVFNDVAVAARLMQAEWRYRGPHSGSLRVGVIDL
                     DVHQGNGTAAIFRDDATVFTFSMHCAKNFPFRKEASDLDLDLPEGCGDADYLDALDAG
                     LECFWARQQDRPPGLVFYLAGADPHEGDRLGRLKLSTAGLAERDRRVFAALRERGIPV
                     VLTMAGGYGRRIEDTVAVQLNTVAEALACWRAWRSA"
     gene            1266..1697
                     /locus_tag="VFV25_05495"
     CDS             1266..1697
                     /locus_tag="VFV25_05495"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY1392805.1"
                     /translation="MSRPEPALRTRYRQFLPIHTRWADNDIYGHINNVVYYSFFDTAV
                     NRYLIESGALDIHDGPVIGLVVETQCHYFAPLGFPQALEAGIRVAHLGRSSVRYEVGI
                     FAEGVQQSAAHGHFVHVYVDRATRRPVDLPTEFRNALQSLL"
     gene            1720..>2704
                     /locus_tag="VFV25_05500"
     CDS             1720..>2704
                     /locus_tag="VFV25_05500"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEY1392806.1"
                     /translation="MDHVLVARAAFPDIIDRLRAHFAVEDNPGDEIWNKAELAARLAG
                     KRGALTTGSERIDAALLDACPGLKAVCNMAVGYNNIDVEACTARGVIVTNTPDVLTET
                     TADFGFALMMAAARRISESEHYLRRGEWTNWRYDMFVGPDVHGATLGVLGMGRIGQAI
                     ARRGALGFGMRVIYHNRSRLAPEIEAGLNARYVDKATLLREADHLVIVVPYSPASHHA
                     IGAAELAQMKPTATLTNIARGGVVDDAALAQALREGRIAAAGLDVFEGEPKVHPDLLA
                     VPNVVLTPHIASASVATRRAMASLAVDNLIAALGCGPQAGRPPTPVNPEVLR"
BASE COUNT          446 a          894 c          925 g          439 t
ORIGIN      
        1 caggccaaag agggtttcga cgttggcctt gtgggcggcg atgatttgtt cggcagtcag
       61 cattgcgagc tccatttagt tggtcagaca gatcagtcgg tttgagactg gggtgctgca
      121 attgttgcgc tgcagcatga agcgaagtat agggtcagtc gggagctttg caagcggtat
      181 ttgttgcgat gcagcaatat agatgcgttt atctaaaagc tccctgcacg gcagtctgtt
      241 gacccgcgat gacaggctga tgaagcggcc cgctcgcaag ccctctatgc tggctggacg
      301 ctcaacagac ccggaccatg ctggccttcc atagcgaccg ctacaccctg ccgctgcccg
      361 aagggcaccg cttcccgctg gcgaagtacc gcatgctgcg tgagctcgtg gcggcgcagc
      421 tgccggccgt gcgccagcag gaggcgccgg ccgccagcga tggcgagctg gtcctggcgc
      481 acgagccgga ctacgtcgca gcggtcgccg aaggctggct cacgccggcg cagcagcgcg
      541 agatcggctt ttcgtggtcg ccccagatgg ccgaacgggc gcggcgctcg gtcggggcga
      601 ccatagtggc ggcgcgcgcc gccttgcggg agggcgtggc ggctaatctg gccggcggca
      661 cccaccatgc ccacgccgac aagggcagcg gctactgcgt cttcaacgac gtggcagtgg
      721 cggcgcggct gatgcaggcc gaatggcgat atcgcgggcc gcacagcggc tccctgcgcg
      781 tgggggtcat cgatctggac gtgcatcagg gcaacggcac ggctgcgatc ttccgcgacg
      841 acgcgaccgt cttcaccttc tcaatgcatt gcgcgaagaa ctttcccttc cgcaaggagg
      901 cgagcgatct ggacctggat ctgcccgagg gctgcggcga cgcggactat ctcgacgcgc
      961 tggatgcagg attggaatgc ttctgggcgc gccagcagga ccggccgccc gggctggtgt
     1021 tctacctggc gggtgccgat ccacacgaag gcgaccgcct gggccgcctg aaactgagca
     1081 ccgctgggct ggccgagcgc gatcggcgcg tgtttgcggc cctgcgcgag cgcggcatcc
     1141 ccgtcgtgct gacgatggcc ggcggctacg gccgccgcat cgaggacacg gtggcggtac
     1201 agctcaacac cgtggccgag gcgcttgcct gctggcgggc ctggcgctcc gcataatccg
     1261 ggggcatgag ccgaccagaa cctgcgctga gaactcgtta ccgccagttc ctccccatcc
     1321 acacccgctg ggccgacaat gacatctacg gccacatcaa caacgtcgtc tactacagct
     1381 tcttcgacac ggcggtgaac cgctatctga tcgagagcgg cgcgctggac atccacgatg
     1441 ggccagtgat cggcctggtg gtcgagacgc aatgccacta tttcgcgccg ctgggcttcc
     1501 cgcaggcgct ggaggcgggg atacgcgtgg cgcatctggg gcgctccagc gtgcgctatg
     1561 aagtcggcat ctttgccgaa ggggtgcagc agtcggcggc gcacggccat ttcgtgcacg
     1621 tctacgtgga ccgcgccacg cgccggcccg tcgaccttcc gactgaattc cgcaacgccc
     1681 tgcagagcct gctctgaact gaacgacaag aggctgaaca tggaccatgt gctggtggcg
     1741 cgcgccgcct ttcccgacat catcgatcgc ctgcgcgccc atttcgccgt cgaagacaac
     1801 cctggcgacg agatctggaa caaggccgag ctggcggcgc gtctggccgg caagcgcggc
     1861 gcactgacca ccggcagcga gcgcatcgac gccgccctgc tcgatgcctg ccccggtctc
     1921 aaggccgtgt gcaacatggc ggtgggatac aacaacatcg acgtcgaggc ctgcaccgcg
     1981 cgcggcgtga tcgtcaccaa cacgcccgat gtgctgaccg agaccacggc cgacttcggc
     2041 ttcgcgctga tgatggccgc cgccaggcgc atcagcgaat cggagcatta cctgcgccgc
     2101 ggcgaatgga ccaactggcg ctacgacatg ttcgtcggtc ccgatgtgca cggcgcgacg
     2161 ctgggcgtgc tgggcatggg ccgcatcggc caggccatcg cgcgccgagg tgcactgggc
     2221 ttcggcatgc gggtgatcta ccacaaccgc tcgcgcctgg cgcccgagat cgaggccggg
     2281 ctgaacgcgc gctatgtcga caaggccacg ctgttgcgcg aggccgatca cctcgtcatc
     2341 gtcgtgcctt attcgccggc ttcgcatcac gcgataggcg cggccgaact ggcgcagatg
     2401 aagcccacgg cgacgctgac caacatcgcg cgcggcggcg tggtcgacga tgcggcgctc
     2461 gcccaggcgc tgcgcgaggg gcgcatcgcc gcggccggcc tcgacgtctt cgagggcgag
     2521 cccaaggtgc atcccgatct gctcgcggtg cccaatgtcg tgctgacccc gcacatcgcc
     2581 agcgcctcgg tcgcgacgcg gcgcgcgatg gcaagcctcg cggtggacaa cctgatcgcg
     2641 gcgctgggct gcgggccgca ggcgggcagg ccaccaacgc cggtgaatcc ggaggtgctg
     2701 agat
//
LOCUS       DASUCY010000285         2217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1564096, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000285 DASUCY010000000
VERSION     DASUCY010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1564096"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /gene="pyrR"
                     /locus_tag="VFV25_05505"
     CDS             <1..384
                     /gene="pyrR"
                     /locus_tag="VFV25_05505"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEY1392807.1"
                     /translation="AERLQRDLGLPGQHGVISSALHRDDFGSRGLLAKADATHLPFEI
                     EGRHVLLIDDVLYTGRTTRAVINELFDFGRPASVTLAVLVDRGGRELPIQPAYSAARV
                     TLPAGERISLARGEDGRFSFHIEPA"
     gene            386..1348
                     /locus_tag="VFV25_05510"
     CDS             386..1348
                     /locus_tag="VFV25_05510"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEY1392808.1"
                     /translation="MLNKRNPQLNAHGELIHLLSIEGLPQAVLHHVLDTAETFLSVNE
                     REVKKVPLLRGKSVFNLFFENSTRTRTTFEIAAKRLSADVINLDIARSSTAKGESLLD
                     TIANLSAMHADMFVVRHSESGAPYLIAQHCAPHVHVVNAGDGRHAHPTQGLLDMYTIR
                     HYKKDFTNLTVAIVGDIVHSRVARSDIHALTTLGVPEVRAVGPRTLVPGDLKDMGVRV
                     CHNLAEGIKDADVVIMLRLQNERMSGAMLPSAGEFFKNFGLTEEKLALAKPDAIVMHP
                     GPINRGVEIDSAVADGRQSVILPQVTFGIAVRMAVMSIIAGNEA"
     gene            1380..2210
                     /locus_tag="VFV25_05515"
     CDS             1380..2210
                     /locus_tag="VFV25_05515"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HEY1392809.1"
                     /translation="MSLIERLRYGNWASRLAVLILLLLLLWWLAPVAIVPAGYRGVIT
                     TFGRASDTVYEPGIHLRFPLAQALHLMDVRIQKGEGEGDAASRDLQSVHTRIAINYNL
                     LPSSAVNAFRDIGPSTDVVADRIIVPATQEAVKAVTARFTAEELITRRTEVRDAIGSL
                     LREKMQRHGLVLDEFAIVNFAFSRSFSEAIEAKVKAEQEKLKAERDLQRIEVEAKQKV
                     ASAKAEAEALALQRQQVTPELLALRRIENERAAIAKWDGKLPQVAGGAMPFINVAPEK
                     "
BASE COUNT          400 a          761 c          725 g          331 t
ORIGIN      
        1 gccgagcggc tgcagcgcga cctgggtctg cccggacagc atggcgtgat ctccagcgcc
       61 ctgcatcgcg acgacttcgg ctcgcgtggc ctcttggcca aggccgacgc cacccacctg
      121 cccttcgaga tcgagggtcg gcatgtgctg ctgatcgacg acgtgctcta caccggccgc
      181 accacgcgcg cggtgatcaa cgaactattc gacttcggcc gcccggccag cgtgacgctg
      241 gcggtgctgg tggatcgcgg cgggcgcgaa cttccgatcc agccggccta ctccgccgcc
      301 cgcgtgacgc tgccggccgg cgagcgcatt tcgctggcgc gcggtgaaga cggccgcttc
      361 agcttccata tcgaacccgc ctgacatgct caacaagcgc aacccccagc tcaacgccca
      421 tggcgagctg atccacctgc tgtccatcga gggcctgccg caggcggtac tgcaccacgt
      481 cctcgacacg gcagagacct ttctgagcgt caacgagcgc gaggtgaaga aggtgccgct
      541 gctgcgcggc aagagcgtgt tcaacctgtt cttcgagaac agcacgcgca cgcgcaccac
      601 cttcgagatc gccgccaagc gcctgtcggc cgacgtcatc aatctcgaca tcgcgcgttc
      661 cagcacggcc aagggcgaga gcctgctcga caccatcgcc aacctgtcgg ccatgcacgc
      721 cgacatgttc gtcgtgcggc acagcgaaag cggagcgccc tatctcatcg cgcagcactg
      781 cgcgccgcat gtgcatgtgg tcaacgccgg cgacgggcgc catgcgcacc cgacgcaggg
      841 cctgctcgac atgtacacga tccgccacta caagaaggac ttcaccaatc tcacggtggc
      901 catcgtcggc gacatcgtgc actcgcgcgt cgcgcgctcc gacatccatg ccctgacgac
      961 gctgggcgtg cccgaggtga gggcggtcgg cccgcgcacg ctggtgcctg gcgacctgaa
     1021 ggacatgggc gtgcgcgtgt gccacaacct cgccgagggc atcaaggatg ccgacgtggt
     1081 catcatgctg cggctgcaga acgagcgcat gagcggcgcg atgctgccca gtgccggcga
     1141 gttcttcaag aacttcggcc tcaccgaaga gaagctggcg ctggccaagc ccgatgccat
     1201 cgtgatgcac ccggggccga tcaatcgcgg cgtcgagatc gactcggcgg tggccgacgg
     1261 caggcagagc gtgatcctgc cccaggtcac cttcggcatc gcggtgcgca tggcggtgat
     1321 gtccatcatc gccggcaacg aagcctgagc ggcctgggcg gaaaaacgaa ggaggggaga
     1381 tgagcttgat cgaacgcttg agatacggca actgggccag ccgcctggcc gtgctgatcc
     1441 tgttgctgct cctgctgtgg tggctggcgc cggtggccat cgtgccggcc ggctatcgcg
     1501 gggtgatcac gaccttcggt cgcgcttccg acacggtcta cgagccgggc atccacctgc
     1561 gtttcccgct ggcgcaggcg ctgcacctga tggacgtgcg catccagaag ggcgaaggcg
     1621 agggcgatgc cgcctcgcgc gacctgcagt cggtgcacac gcgcatcgcg atcaactaca
     1681 acctgctgcc cagctccgcc gtcaatgcct tccgcgacat cggccccagc accgacgtgg
     1741 tggctgaccg catcatcgtg ccggccaccc aggaagccgt gaaagccgtg acggcacgct
     1801 tcacggccga agagctgatc acgcgccgca ccgaggtgcg cgacgcgatc ggcagcctgc
     1861 tgcgcgagaa gatgcagcgc cacggcctgg tgctcgacga gttcgcgatc gtcaattttg
     1921 ccttttcgcg cagcttcagc gaggcgatcg aagccaaggt caaggccgag caggaaaagc
     1981 tcaaggccga gcgcgacctg cagcgcatcg aggtcgaggc caagcagaag gtggccagcg
     2041 ccaaggccga ggccgaggcg ctggcgctgc agcgccaaca ggtcacgccc gagctgctgg
     2101 ccttgcgccg catcgagaac gagcgcgcgg cgatcgccaa gtgggacggc aaactgccgc
     2161 aggtcgccgg cggggccatg cccttcatca acgtggcgcc cgagaaatag caagaac
//
LOCUS       DASUCY010000286         2211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_391555, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000286 DASUCY010000000
VERSION     DASUCY010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391555"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..778
                     /locus_tag="VFV25_05520"
     CDS             125..778
                     /locus_tag="VFV25_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEY1392810.1"
                     /translation="MTRRFEDARAQTLGEELANAISHGIGFLLAVASLPILVVFAAQH
                     GGPRNVAAAVVFSSTMMLLYFASAMYHALPPGRAKRVFNKLDHAAIYVFIAGSYTPFA
                     LGALRGSWGWSLFGLVWGLAMLGVLLKSFNRLQHPGWSTALYVAMGWLVIIAAVPLVQ
                     HVSLNGLLLLVAGGIAYTAGAVFFLLDSRLRYGHFLWHLFVMGGSACHFFAALYHAR"
     gene            848..1573
                     /locus_tag="VFV25_05525"
     CDS             848..1573
                     /locus_tag="VFV25_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY1392811.1"
                     /translation="MAGHSKWANIQHRKGRQDEKRGKAWTRVIREIMVAARVGGGDPS
                     ANPRLRLAIEKAKAVNLPADTVKRNIDKATGNLEGVNYEEIRYEGYGIGGAAIIVDCM
                     TDNRVRTVAEVRHAFSKYGGNLGTEGSVAFQFKHVGQFVFAPGTGEDKVMEAALEAGA
                     DDVVSDEDGAIEVLCAPADFEAVKQALEAAGLKPDVAEVTMRAENSIELSGEDAQRMQ
                     KLLDVIEDLDDVQDVFHNAVINE"
     gene            1627..>2211
                     /locus_tag="VFV25_05530"
     CDS             1627..>2211
                     /locus_tag="VFV25_05530"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458747.1"
                     /note="catalyzes the formation of
                     N(1)-(5-phospho-D-ribosyl)glycinamide from
                     5-phospho-D-ribosylamine and glycine in purine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEY1392812.1"
                     /translation="MKVLVIGGGGREHALAWKIAQSPRVQMVYVAPGNGGTAQDVRLM
                     NVAITDVKALADFAQTEKMALTVVGPEAPLAAGVVDEFRARGLRIFGPSRAAARLESS
                     KAFAKDFMQRHGIPTALYATFSDVAEAHAHLDKVGVPIVIKADGLAAGKGVVVATTLE
                     QAHEAIDWMLLDNKLGVQHNEGGAQVVIEEFLQGE"
BASE COUNT          376 a          713 c          766 g          356 t
ORIGIN      
        1 tggccaatct ggtgattgcg gcgctcgagc ccgacacgca gaacggctac ttcgccaacc
       61 acctgctacg ctcgctcgac gatgccgcaa gggtcatgac cgcgatcaag ctggaggatc
      121 cccgatgaca cgccgctttg aagacgcccg cgcgcagacg ctgggcgagg agctggccaa
      181 cgcgatcagc cacggcatcg gcttcctgct cgcggtggcc tcgctgccga tcctcgtcgt
      241 cttcgccgcg cagcacggcg gcccgcgcaa cgttgccgcg gccgtcgtgt tctcgtcgac
      301 gatgatgctg ctgtatttcg cgtcggcgat gtaccacgca ctgccgccgg ggcgcgccaa
      361 gcgcgtcttc aacaagctcg accatgcggc gatctacgtc ttcatcgccg gcagctacac
      421 gccgttcgcg ctcggtgcat tgcgcggcag ctggggctgg agcctgttcg gcctggtgtg
      481 ggggttggcg atgctcggcg tgctgctgaa gagcttcaat cgcctgcagc accccggctg
      541 gtcgacggcg ctgtacgtgg ccatgggctg gctggtgatc atcgcggctg tgcccttggt
      601 tcagcacgtg tcgctcaacg gcttgctgct gctcgtcgcc ggcggcattg cctacactgc
      661 gggcgcggtg ttcttcctgc tcgactcgcg gctgcgctac ggccacttcc tctggcacct
      721 gttcgtcatg ggcggcagcg cctgccactt cttcgcggcg ctgtaccacg cgcggtgagc
      781 ccggccggcc cacgtaaaat cgaccctcta ccaacagagg gatcgccagc gagcgacagg
      841 agccttcatg gccggacatt ccaaatgggc caacatccag caccgcaagg gccgccagga
      901 cgagaagcgc ggcaaggcct ggacgcgcgt gatccgcgag atcatggtgg ccgcccgcgt
      961 cggcggcggt gacccgtcgg ccaacccgcg cctgcggctg gccatcgaga aggccaaggc
     1021 ggtgaacctg ccggccgaca cggtcaagcg caacatcgac aaggccaccg gcaacctcga
     1081 aggcgtcaac tacgaggaaa tccgctacga gggctacggc atcggcggcg ccgcgatcat
     1141 cgtcgactgc atgaccgaca accgggtgcg cacggtcgcc gaggtgcgcc atgcgttcag
     1201 caagtacggc ggcaacctgg gcaccgaggg ctcggtggcc ttccagttca agcatgtggg
     1261 ccagttcgtg ttcgcgcccg gcaccggtga ggacaaggtg atggaagcgg cactggaagc
     1321 cggcgccgac gatgtggtca gcgatgagga cggcgccatc gaggtgttgt gcgcgccggc
     1381 cgatttcgaa gccgtgaagc aggcgctgga ggcggccggt ctcaagccgg atgtggccga
     1441 ggtgacgatg cgcgccgaga actccatcga actgagcggc gaagacgcgc agcgcatgca
     1501 gaagctgctg gacgtcatcg aggatctcga cgacgtgcag gacgtattcc acaacgcagt
     1561 gatcaacgag tgagtagcgg cgtctcgtcg tgtcgcttcc tgcctataaa agactgaggg
     1621 tttagcttga aagttctcgt gatcggcggc ggcggccgcg aacatgcgct ggcctggaag
     1681 atcgcgcagt cgccgcgcgt gcagatggtc tatgtcgctc cgggcaacgg cggcacggcg
     1741 caggacgtgc gcctgatgaa cgtggccatc accgacgtga aggcgctggc cgacttcgcg
     1801 cagaccgaga agatggcgct caccgtggtc ggccccgagg cgccgctggc ggccggcgtg
     1861 gtggacgagt tccgcgcccg cggcctgcgc atcttcgggc ccagccgggc cgcggcccgg
     1921 ctcgaaagct ccaaggcctt cgccaaggac ttcatgcagc gccacggcat acccacggcg
     1981 ctgtatgcca ccttcagcga tgtggccgag gctcatgcgc atctggacaa ggtcggcgtg
     2041 cccatcgtga tcaaggccga cggcctggcc gcgggcaagg gcgtggtggt ggccacgacg
     2101 ctggagcagg cgcacgaggc gatcgactgg atgctgctgg acaacaagct gggcgtgcag
     2161 cacaacgagg gcggtgcaca ggtcgtgatc gaagagttcc tgcagggcga g
//
LOCUS       DASUCY010000287         2039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_520260, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000287 DASUCY010000000
VERSION     DASUCY010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520260"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="VFV25_05535"
     CDS             <1..967
                     /locus_tag="VFV25_05535"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEY1392813.1"
                     /translation="VAYHEDSLRFWLNRGVDGFRLDAVPHMIENGPKDWNDQPESRAL
                     TGELRKLITGYARHYVVCEATANPQAYAAPEVCGSAFAFGGHGSDIVEAAKGKPEAIR
                     AVADYFKTAPASMATMVSNHDIFAGQRLWDQVGGDVAQYKLAAATYLLQPGTPFIYYG
                     EEIGMGGVNSLPGDEPLRSPMSWSADANSGFTSGQPFRPLAPNAATHNAAAEQADPHS
                     LLAFYKNMLRLRNTLPSIAQGSYEAAQVEGQTLSYQRKFKREHSVVLLNYGTEPAELT
                     VPGLHAGARLKSAYPRGWKSLKADRQGVVRVRMPAQSVRVLVVQR"
     gene            complement(981..1685)
                     /locus_tag="VFV25_05540"
     CDS             complement(981..1685)
                     /locus_tag="VFV25_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY1392814.1"
                     /translation="MSTATPLTLVSHALCPYVQRAAIVLHEKGVPFERRDVDLANKPA
                     WFLEVSPLGKTPVLLVDGDTAIFESAVICEYLDEVHAPRLHPPDPLQRARHRAWMEFG
                     SGVLNGIAAFYNAPDEAQLRARRDDLRAKFAQLEAQLAQHPAVDGGPWFDGERFSLVD
                     AVFAPVFRYFEVFEAETGEDFGAFSHTPRVARWRAHLAQRPSVRQAVASDYVQRLAQF
                     LRQRGSALAKRMRVAA"
     gene            complement(1682..>2039)
                     /locus_tag="VFV25_05545"
     CDS             complement(1682..>2039)
                     /locus_tag="VFV25_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEY1392815.1"
                     /translation="ARQPRALRGAAVISFVSSELHKAFSPWLWHKETADSTVEAVKEK
                     IAARFAELDQRLATRSYLMGEQFTVADAYCYVVANWSRLLGIGLEPYPALDVYLQRVA
                     ARPKVREALQAEGLLK"
BASE COUNT          355 a          715 c          672 g          297 t
ORIGIN      
        1 cgtggcctat cacgaggaca gcctgcgctt ctggttgaac cgcggcgtgg atggcttccg
       61 cctcgatgcc gtgccgcaca tgatcgagaa cggacccaag gactggaacg accagcccga
      121 gagccgcgcg ctcaccggcg agctgcgcaa gctgatcacg gggtatgcgc gccactatgt
      181 ggtctgcgaa gccaccgcca atccccaggc ctatgccgcg cccgaggtct gcggcagcgc
      241 cttcgccttc ggcgggcatg ggagcgacat cgtcgaggcc gcaaagggca agcccgaggc
      301 aatccgcgcc gtggccgact acttcaagac cgcgccggcc agcatggcga ccatggtgtc
      361 caaccacgac atcttcgccg gccagcgcct gtgggaccag gtgggcggcg atgtagccca
      421 gtacaagctg gccgccgcga cctatctgct gcagcccggc acgcccttca tctactacgg
      481 cgaggaaatc ggcatgggcg gcgtcaacag cctcccgggc gacgagccgc tgcgcagccc
      541 gatgagctgg agcgccgacg cgaatagcgg cttcacgagc ggccagccct tccgccccct
      601 cgcacccaac gccgcgacgc acaacgccgc cgccgagcag gccgacccgc attcgctgct
      661 cgccttctac aagaacatgc tgaggctgcg caacaccctg ccttcgatcg cccagggcag
      721 ctatgaagcg gcccaggttg aggggcagac gctgagctac cagcgaaagt tcaagcgcga
      781 gcacagcgtg gtgctgctga actacggcac ggagccggcc gagctcaccg tgcctggact
      841 gcacgcgggg gctcgcttga agagcgccta cccgcgcgga tggaagtcgc tcaaggccga
      901 taggcagggg gtggtgaggg tgaggatgcc ggcgcagtcg gtgcgggtgc tggtggtgca
      961 gcgctagggc ttgctggccg tcacgcggcc acgcgcatgc gcttagccag cgcgctgccg
     1021 cgctggcgca ggaactgcgc cagcctttgc acatagtcgc tagccacggc ctgccgcacc
     1081 gaaggccgct gcgccaaatg cgcccgccag cgcgcgacgc gcggcgtgtg cgagaaagcg
     1141 ccgaagtcct ctcctgtctc ggcctcgaac acctcgaagt agcggaacac cggcgcaaac
     1201 accgcatcga ccaggctgaa gcgctcgccg tcgaaccagg ggccgccgtc gactgcaggg
     1261 tgttgcgcca gctgcgcttc gagctgcgcg aacttcgccc gcagatcgtc gcgcctggcc
     1321 cgcagttgag cctcgtcggg cgcgttgtag aacgccgcga tgccgttgag cacgcccgag
     1381 ccgaactcca tccaggcgcg gtgccgggcg cgctgcagcg gatcgggcgg atgcaggcgc
     1441 ggcgcatgca cctcgtccag gtactcgcag atcacggccg attcgaagat cgcggtgtcg
     1501 ccgtccacca acagcaccgg cgtcttgccc agcggcgaga cctcgaggaa ccaggccggc
     1561 ttgttggcga gatcgacgtc gcgccgctcg aagggcacgc ccttctcgtg cagcacgatg
     1621 gccgcgcgct gcacataagg gcacagcgcg tggctcacca acgtgagagg tgtcgctgtg
     1681 ctcatttcaa cagaccttcg gcctgcagcg cctcgcgcac cttaggccgc gcagccacgc
     1741 gctgaagata gacgtccagc gccggatacg gctccagacc gatgcccagc aaacggctcc
     1801 agttggcaac cacatagcag tacgcgtcgg ccaccgtgaa ctgctcgccc atcaggtagc
     1861 tgcgcgtggc cagccgctgg tcgagttcgg cgaagcgcgc tgcgatcttc tccttcacgg
     1921 cctccacggt ggaatcggcc gtctccttgt gccacagcca ggggctgaag gccttgtgca
     1981 gctcgctgct gacgaagctg atcactgctg cacctcgaag cgctcgcggc tgccgtgcc
//
LOCUS       DASUCY010000288         2220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_969968, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000288 DASUCY010000000
VERSION     DASUCY010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969968"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..>2220
                     /locus_tag="VFV25_05550"
     CDS             18..>2220
                     /locus_tag="VFV25_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HEY1392816.1"
                     /translation="MNAPLLLTQITGQTEEDAAPRLREIPYNYTSFSDREIVIRLLGE
                     RAWEVLNRLREERRTGRSARMLYEVLGDIWVVQRNPYLQDDLLDNAKRRALLTDALHH
                     RLREIEKRRTPVAASADPESLAAAQRDGFVGELLTQAQRAVERFAGQFREIGALRQRT
                     RRVLGRCTARDNIKFDGLSRVSHVTDATDWRVEYPFVVLTPDSEAEMAALVKGCIELD
                     LTIIPRGGGTGYTGGAVPLTWKSAVINTEKLEAMSEVEMLRLPGLDREVATVWTEAGV
                     VTQRVADAAERAGYVFAVDPTSAEASCVGGNVAMNAGGKKAVLWGTALDNLASWRMVT
                     PEAKWLEVVRLDHNLGKIHDVESARFELRYFDASGKKLERTEQLEIPGRVFRKEGLGK
                     DVTDKFLAGLPGVQKEGCDGLITSARWVVHRMPRHTRTVCLEFFGNPKDAVPSIVEIK
                     DYMFAQAKLPGGAILAGLEHLDDRYLKAVGYTTKSKRAVGGNGLPKMVLVGDIVGDDE
                     DAVARATSEVVRMANGRSGEGFVAVSADARKKFWLDRKRTAAIAKHTNAFKINEDVVI
                     PLPRMGEYTLGIERINIELSLRNKLALLDRLEAWFGAGSLPLGKTDDASEIPSAELLE
                     DRVQQALAIVRETRELWRNWLTQLDAAPADGGPSYFDQLQDHSLRASWKTQVLKALQA
                     IVAGAAFAPIIEEARRIHKEVLRGRVWVALHMHAGDGNVHTNIPVNSDNYEM"
BASE COUNT          389 a          744 c          772 g          315 t
ORIGIN      
        1 ttgcgcgcct tgcgcccatg aacgcaccgc tcctgctgac ccagatcacc ggccagaccg
       61 aagaagacgc agcgccccgc ctgcgcgaga ttccctacaa ctacacctcg ttttccgacc
      121 gggagatcgt gatccggctg ctgggggagc gcgcctggga ggtgctgaac cggctgcgcg
      181 aggagcgccg caccggccgc tcggcccgca tgctctatga agtgctgggc gacatctggg
      241 tggtgcagcg caatccctat ctgcaggacg acctgctgga caacgccaag cggcgcgccc
      301 tgctgaccga tgccttgcac catcgcctgc gcgagatcga gaagcgccgc acccccgtgg
      361 ccgcgtcggc cgatccggaa agcctggctg ctgcgcagcg cgacggcttc gtcggcgaac
      421 tgctgaccca ggcccagcgg gctgtggagc gcttcgccgg ccagttcagg gagatcggcg
      481 cgctgcggca gcgcacgcgc cgcgtgctgg ggcgctgcac ggcgcgcgac aacatcaagt
      541 tcgacgggct gagccgcgtc tcccatgtga cggacgcgac cgactggcgc gtcgaatatc
      601 ccttcgtcgt gctgacgccc gacagcgaag ccgagatggc cgcgctggtg aaaggctgca
      661 tcgagcttga cctgaccatc atcccgcgcg gcggcggcac cggctacacc ggcggcgccg
      721 tgccgctgac ctggaagagc gccgtcatca acaccgagaa gctggaggcg atgagcgagg
      781 tcgagatgct gcgcctgccc gggttggatc gcgaggtcgc gacagtctgg accgaggccg
      841 gtgtcgtgac ccagcgcgtg gccgatgccg ccgagcgcgc cggctacgtc tttgcggtgg
      901 acccgacctc tgccgaagcc tcctgcgtgg gcggcaacgt cgcgatgaat gcgggcggca
      961 agaaagcggt gctgtggggc acggcgctgg acaacctggc ctcctggcgc atggtgacgc
     1021 ccgaggcgaa atggctggaa gtggtgcgcc tcgaccacaa cctgggcaag atccacgatg
     1081 tcgagtcggc ccgcttcgag ttgcgctatt tcgacgcctc gggcaagaag ctggagcgca
     1141 ccgagcagct cgagatcccg ggccgcgtct tccgcaagga gggcctgggc aaggacgtga
     1201 ccgacaagtt cctggccggc ctgccgggcg tccagaagga gggctgcgac ggcctgatca
     1261 ccagcgcgcg ctgggtcgtg caccgcatgc ccaggcacac gcgcacggtc tgcctggagt
     1321 tcttcggcaa cccgaaggac gcggtgcctt ccatcgtcga gatcaaggac tacatgttcg
     1381 cgcaggcgaa gctgccgggc ggcgccatcc tggccggcct ggagcacctg gacgaccgct
     1441 atctgaaggc cgtgggctac accacgaaga gcaagcgcgc agtgggtggc aatggactgc
     1501 ccaagatggt gctggtgggg gatatcgtcg gcgacgacga ggatgccgtg gcgcgcgcca
     1561 ccagcgaggt ggtgcgcatg gccaacggcc gcagcggcga gggttttgtc gcggtgagcg
     1621 ccgacgcccg caagaagttc tggctggacc gcaagcgcac cgccgcgatc gccaagcaca
     1681 ctaacgcctt caagatcaat gaagacgtgg tgatcccgct gccgcgcatg ggcgagtaca
     1741 cgctgggcat cgagcgcatc aacatcgagc tctccttgcg caacaagctc gccctgctgg
     1801 accgcctcga agcctggttc ggcgccggca gcctgccgct gggcaagacg gacgacgcga
     1861 gcgagatccc cagcgccgag ctgctggaag accgcgtgca gcaggcgctg gccatcgtgc
     1921 gcgagacgcg cgagctgtgg cggaattggc tcacccagct cgacgccgcg ccggccgacg
     1981 gcggcccgag ctacttcgac cagctgcagg accacagcct gcgcgcctcg tggaagaccc
     2041 aggtgctgaa agccctgcag gcgatcgtcg ccggcgcggc cttcgcaccc atcatcgagg
     2101 aagccaggcg catccacaag gaggtgctgc gcggccgcgt gtgggtggcg ctgcacatgc
     2161 atgccggcga cggcaatgtg cacaccaaca tcccggtcaa ctccgacaac tatgagatgc
//
LOCUS       DASUCY010000289         3163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_761231, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000289 DASUCY010000000
VERSION     DASUCY010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761231"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..122
                     /locus_tag="VFV25_05555"
     CDS             <1..122
                     /locus_tag="VFV25_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HEY1392817.1"
                     /translation="ARSILLHGDTPGAVALARTVRSEIEAAGGRIVPVSRLFA"
     gene            234..986
                     /locus_tag="VFV25_05560"
     CDS             234..986
                     /locus_tag="VFV25_05560"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY1392818.1"
                     /translation="MPFSDYKTALVTGASSGIGAAVVERLRKEGLEVHAIARSASKLD
                     ELAARTGCIAHAIDVTDIAGITRLAKEVEFDVLVNNAGVDRPQSILKAAPQDIDLLVD
                     VNLRAVLHLCNLVVPGMVARDRGHVVNISSIAGAYNFGGNSTYHATKAAISMLSRQLR
                     IDAFGKRVRVTEICPGRVATDIFSHVHGDSPEIRERFIEGFELPEASDIANAIAFAIA
                     APVAVNIGHMEITPTLQVPGGLSTTRPETPRA"
     gene            1018..1719
                     /locus_tag="VFV25_05565"
     CDS             1018..1719
                     /locus_tag="VFV25_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003263786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY1392819.1"
                     /translation="MKGFDLIAILVNPEFVKMLWHGVQMTLIIALGSWLVAMGLAVVL
                     LVVRLTPSRIAGRVVEAYVSYHRNVPTLVQLMMWYFGIASLLPDSLQAWLGEHDGEAV
                     FSIIALGLCQAAYFSEDLRSGLRSIPPGQAEAARALGHSFIGAMRHVLMPQAVRNALP
                     AVVNHSVSLFKNSSLAMAIGVAELTHAVKEIENQSFRAFETYLMATVMYLGFSLLIMA
                     AGAWLARRMRIVGAR"
     gene            1723..2481
                     /locus_tag="VFV25_05570"
     CDS             1723..2481
                     /locus_tag="VFV25_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY1392820.1"
                     /translation="MGAIIDILRDNWLLLLIGQYPNGPLGGIACTLILSVLGILLAFP
                     VSVLLALARLSRWRWLKWPATALVYLARGVPLLMFILWVYFFVPLLIGREVSGFTTML
                     CTLVIYEGAYLAEVVRAGIEALPKGQMEAARALGHGYLGAMRFVILPQALFNMLPSML
                     SHFVSTIKETTLGYVINVPELTFAASQINNQLLTKPFQVFFILAAIYYVVCWSLTRVA
                     IALERRIEAKRAGLRRAKGEAPAAVAAEPLATEP"
     gene            2497..>3163
                     /locus_tag="VFV25_05575"
     CDS             2497..>3163
                     /locus_tag="VFV25_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008165223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEY1392821.1"
                     /translation="MITFSHVDKWYGSYRALTDINAEVKKGEVVVVCGPSGSGKSTLI
                     RTVNRLEEIKSGQVLVDGHDIHAPMGGAQLNKLRSRIGFVFQSFNLFPHLSALDNVML
                     SPVHVNGAKRAEARDKAMQLLERVGLANKAASYPAQLSGGQQQRVAIARALAMEPPAM
                     LFDEPTSALDPEMVGEVLAVMRSLAAEGMTMMCVTHEMNFAREVADRVWFMDHGRLIE
                     TGTP"
BASE COUNT          511 a         1052 c         1050 g          550 t
ORIGIN      
        1 acgcgcgctc gatcctgctg cacggcgaca cgccgggtgc ggtggcgctg gcgcgcacgg
       61 tgcgcagcga gatcgaggcg gccgggggcc gcatcgtgcc ggtctcgcgc ctcttcgctt
      121 gagcagggtt cccgcctaca taagaccgtc ttatcgcacc acacccattc cgtcttggcc
      181 tattcgttcc gcaatcgata gatttgcttc acgtcacgag gaattcacaa atcatgccgt
      241 tctccgacta caagacagcc ctcgtcaccg gagcctcctc cggtatcggc gccgccgtcg
      301 tcgagcgcct gcgcaaggaa ggtctcgagg tccacgcgat cgcgcgcagc gcaagcaagc
      361 tggacgaact ggcggcgcgc accggatgca ttgcgcatgc gatcgacgtg accgacatcg
      421 caggcatcac gcggctcgcc aaagaggtgg agttcgacgt gctggtcaac aacgccggcg
      481 tcgaccggcc gcagtcgatc ttgaaggccg cgccgcagga catcgacctg ctggtcgacg
      541 tcaacctgcg cgccgtgctg cacctgtgca acctcgtcgt gcccggcatg gtggcgcgcg
      601 accgcgggca tgtggtgaac atcagctcga ttgccggcgc ctacaacttc ggcggcaact
      661 cgacctatca cgcgaccaag gcggcgatca gcatgctgtc gcgccagctg cgcatcgacg
      721 ccttcggcaa gcgcgtgcgc gtcaccgaga tctgcccggg ccgcgtggcc accgacatct
      781 tttcgcatgt gcacggcgat tcgccggaga tccgggagcg cttcatcgaa ggcttcgaac
      841 tacccgaggc gtccgacatc gccaatgcaa tcgcctttgc gatcgccgcg ccggtggcgg
      901 tgaacatcgg ccacatggag atcacgccga cgctgcaggt gccgggcgga ctgtctacaa
      961 cgcggcccga gacgccccgg gcctgaaacg aacgcaacgc acgccggaga ctgaaccttg
     1021 aagggcttcg atctcatcgc gatcctggtg aaccccgagt tcgtcaagat gctctggcac
     1081 ggcgtgcaga tgacgctgat catcgcgctc ggctcgtggc tggtggcgat gggcctcgcc
     1141 gtcgtgctgc tggtggtgcg cctcacgccg agccgcatcg ccgggcgcgt tgtcgaggcc
     1201 tacgtctcct accaccgcaa cgtgccgacg ctggtgcagc tgatgatgtg gtacttcggc
     1261 atcgcaagcc tgttgccgga ttccctgcag gcctggctcg gcgagcacga cggtgaggcc
     1321 gtcttctcga tcatcgcgct cggcctgtgc caggccgcct acttcagcga agacctgcgt
     1381 tccgggctgc gctccatccc gcccggccag gccgaagcag cacgcgccct cggtcacagc
     1441 ttcatcggtg caatgcgcca cgtgctgatg ccgcaggcgg tgcgcaacgc gctgcctgcg
     1501 gtcgtcaacc acagcgtgtc gctgttcaag aacagcagct tggccatggc catcggcgtg
     1561 gccgaactga cgcacgcggt gaaggagatc gagaaccaga gcttccgcgc cttcgagacc
     1621 tacctgatgg cgaccgtgat gtacctcggg ttctcgctgc tcatcatggc cgccggcgca
     1681 tggctggcac ggcgtatgcg catcgtcggg gcgaggtagg cgatgggcgc gatcatcgac
     1741 atcctgcgcg acaactggct gctgctgctg atcggccagt atccgaacgg accactcggc
     1801 ggcatcgcct gcacgctgat cctgtcggtg ttgggcatcc tgctcgcctt cccggtgagc
     1861 gtgctgctcg cgctggccag gctgtcgcgc tggcgctggc tcaagtggcc ggcgaccgcg
     1921 ctggtctacc tggcgcgcgg cgtgccgctg ctgatgttca tcctgtgggt gtacttcttc
     1981 gtgccgctgc tcatcggccg cgaggtgtcg ggcttcacga cgatgctttg cacgctggtg
     2041 atctacgagg gagcctatct ggccgaggtg gtgcgcgccg gcatcgaggc cctgccgaag
     2101 gggcagatgg aggcggcgcg cgcgctcggc cacggctacc tgggcgccat gcggttcgtg
     2161 atcctgccgc aggcgctgtt caacatgttg ccgagcatgt tgagccactt cgtgtcgacg
     2221 atcaaggaaa cgacgctggg ctatgtgatc aacgtgcccg agctcacctt cgcggccagc
     2281 cagatcaaca accagctgct gaccaagccg ttccaggtgt ttttcatcct ggccgccatc
     2341 tattacgtcg tctgctggag cttgacgcgt gtcgccattg cgctggagcg ccgcatcgag
     2401 gccaagcggg ccggcctgcg ccgtgcgaag ggtgaggcgc cggctgcggt cgccgcggag
     2461 ccgctcgcaa ccgaaccctg atcgaggaac acggacatga tcacgttctc gcacgtcgac
     2521 aagtggtacg gcagctaccg tgccctcacc gacatcaacg ccgaggtcaa gaagggcgag
     2581 gtggtggtgg tgtgcggccc gtcgggctcg ggcaaatcga ccctgatccg cacggttaac
     2641 cggctggagg agatcaagtc cggtcaggtg ctcgtcgacg gccacgacat ccacgcgccg
     2701 atgggcggcg cgcagctcaa caagctgcgc agccgcatcg gcttcgtgtt ccagagcttc
     2761 aacctgttcc cgcacctgtc ggcgctggac aacgtgatgc tctcgcccgt gcacgtgaac
     2821 ggcgccaagc gcgcggaggc acgcgacaag gcgatgcagc tgctggagcg cgtgggcctg
     2881 gccaacaagg ccgcgtcgta tccggcgcag ctgtcgggcg gccagcagca gcgcgtggcc
     2941 atcgcgcgcg ccctggccat ggagccgccc gcgatgctgt tcgacgagcc caccagcgcg
     3001 ctcgaccccg agatggtggg cgaggtgctg gccgtgatgc gcagcctggc cgccgagggc
     3061 atgacgatga tgtgcgtcac ccacgagatg aattttgcgc gcgaggtggc tgaccgggtc
     3121 tggttcatgg accacgggcg cctgatcgag accggcacgc cgg
//
LOCUS       DASUCY010000290         6490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_102543, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000290 DASUCY010000000
VERSION     DASUCY010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 6490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6490
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102543"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VFV25_05580"
     CDS             complement(<1..741)
                     /locus_tag="VFV25_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEY1392822.1"
                     /translation="MLGPLVEWPAALLVLADVAVLFSGVFARFVLRQPLVWSDELASI
                     LFLWLAMLGAVVALRRGEHMRMTALVGRCSESRRAMLEAVATGACLAFLALVAWPSWQ
                     YAADEQAITTPALEISNAWRAAALPVGVALMAVFAALRLLREARTRQALQAVVAVALI
                     VAAFWLAGSWLDGLGRLNLLIFFVGVAAGCVFAGIPIAFAFGLATFGYLALATGTPLP
                     VMVGRMDEGMSHLILLAVPLFVFLGLLIE"
     gene            complement(822..1826)
                     /locus_tag="VFV25_05585"
     CDS             complement(822..1826)
                     /locus_tag="VFV25_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY1392823.1"
                     /translation="MSLTRRSLLAGAGALALSTGRAQAQRAEFSYKYANNLPVSHPMN
                     LRAKEMVDAIRADTKGRVDIQIFPNNQLGGDTDMLSQVRSGGIEFFTLSGLILATLVP
                     AAAISGIGFAFPDYDTVWKALDGELGAYVRAQIAKAGLVAMDKIWDNGFRQITTSTHP
                     VQTADDLKGMKLRVPPSPLWTSMFKALDAAPASINFAEVYSALQTKVVDGQENPLAVI
                     ATAKLYEVQKYCSLTNHMWDGFWFLANRKAWERLPEDLRAIVARHINAAGLKERADVA
                     ALNASLQKDLAAKGMVFNQPKTDSFRERMRKAGFYAEWKAKFGDEAWALLERSTGKLG
                     "
     gene            1938..2732
                     /locus_tag="VFV25_05590"
     CDS             1938..2732
                     /locus_tag="VFV25_05590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY1392824.1"
                     /translation="MTQQASSTPVAVVTGGARGIGLAVARWFLAHEHRVALLDIDADT
                     LGRTEAALDDPQRVLALHCDVSQPGQVQAAVAAVAQRYGRIDALVNNAGVAVFKPIAD
                     TSYEEWRHVLGTNLDGAFLCTQACAPLMRAGGGGSIVNIASISGLRASTLRVAYGTSK
                     AALIHLTKQQAVELGNVGIRVNVIAPGPVDTEMAKLVHSVAIRSDYYDAIPLNRYGTP
                     EEIAEAVGFLCSPAASYVNGQLLAVDGGFDACGVGLPTLRKNAAAS"
     gene            2744..3994
                     /gene="pcaD"
                     /locus_tag="VFV25_05595"
     CDS             2744..3994
                     /gene="pcaD"
                     /locus_tag="VFV25_05595"
                     /EC_number="3.1.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047570 - 3-oxoadipate enol-lactonase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate enol-lactonase"
                     /protein_id="HEY1392825.1"
                     /translation="MSYDPYDHDLERGMRNRRGILGDAWVDKSVANANSFNAEFQNFI
                     TRYAWHEIWGRPGLDPKTRRIIVLSITCALGRWEEYELHLRAALSAGEDTRLTPDDVK
                     EVLMQAAIYAGVPAANTGVAIAVRLMRELGMELPPLPATEVSHPGVGREGRTASRPAL
                     HYTLREPRNGQAPRHTIVLAHALGCDLMMWDALANTLAADCRVISFDARGHGGSDAPA
                     GPYTMAELAEDAERLLDELDADPVVWLGLSMGGMLGQELALRHPQRLKALVIANSTSG
                     YPPEGRAIWDQRIAAIEAGGLETVVDGTMQRWFHQGFHAAQPATVARWRRRVVTTDEQ
                     GYLAACAAVKAHDTTARLPRIKLPTLIIAGELDQGTPVAMAELMARQIPGSRLVVLKE
                     ASHLSALEQPEAFEAAVRDFLATL"
     gene            complement(4013..6352)
                     /locus_tag="VFV25_05600"
     CDS             complement(4013..6352)
                     /locus_tag="VFV25_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY1392826.1"
                     /translation="MTTPIEAGRYGSGHAVRRVEDPALVAGRGQYADDVSLPGQTHLA
                     FLRSPYAHARIKSIDVSTAAGMPGVLAVFTGEQLVKAGVKPMAGPGFARADGSPGATA
                     PRRALAHEVVRFVGEAVAAVVADSREAARAAADALWVDYEELPAVVDTMAAMQPGAPV
                     LCAQAPDNIAAEMRHGDARAAEAALASAAHRVMLDIDNQRLAPSPMEPRSVLAAFDAG
                     RLTMRLSSQMPTAVRGGLCDTIPGLTPEKVRVLVGDVGGGFGMKTGPYPEDIAVGYAA
                     LQLKRPVKWQAERLEEFVSALHGRDVLSRAELALDASGRVLALRVRSYANVGAYATGA
                     GVAIQLLIGPWVSTSIYDIPVIDLHFSAVMSNRAPTGPYRGAGRPEAIYLIERLMDAA
                     ARKLGLNPAELRRRNMIKPEQMPYKNAMGQTYDSGAFEKILDQGLKLADWDGYATRRE
                     ASKRKGKLRGRGIATFLEWTGGNAFEERVEVTVTPDGFIEIVSATQQMGQGIQTSYAQ
                     LAVDVFGVPIERIRIVQGDTDRANGFGSAGSRSLFTGGSSVSIASQKTIVHAKDLAAQ
                     ALEAPAADIEYRAGRFRVAGTDLGIDLFELAGRQSEARIHVDSTSSVSGPSWPNACHV
                     CEVEIAPDTGEVEIVAYASVNDIGRVVSPVIVRGQIEGGAMQGIGQALCERVRYDAGS
                     GHLLSASFMDYALPRVDTFRGFKTEFDTSIPCKTNPLGVKGVGELGTIGATPAVVNAI
                     VDALDHAGLGADAERIQMPVTSERIWRALARDFEPSPFA"
     gene            complement(6349..>6490)
                     /locus_tag="VFV25_05605"
     CDS             complement(6349..>6490)
                     /locus_tag="VFV25_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011488437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY1392827.1"
                     /translation="LLQHNANPSEAEIVQALEGNLCRCTGYVNIVAAVKQAAAAMQGA
                     KA"
BASE COUNT         1001 a         2269 c         2232 g          988 t
ORIGIN      
        1 ctcgatcagg aggccgagga agacgaacag cggcacggcc aacaggatca ggtgcgacat
       61 gccctcgtcc atgcggccta ccatcaccgg cagcggcgtg ccggtggcca gtgccaggta
      121 gccgaaggtc gcgaggccga aggcgaaggc gatcgggatg ccagcgaaca cgcagcccgc
      181 tgcgacgccg acgaagaaga tcagcaggtt gagccgcccg agcccatcca accaggagcc
      241 cgcgagccag aaggccgcca cgatgagcgc gaccgcgacc accgcctgca gcgcctgcct
      301 agtcctcgcc tcgcgcagca ggcgcagcgc cgcgaagacc gccatcagcg ccacgcccac
      361 cggcagcgcg gcggcgcgcc aggcattgga gatctccagc gccggcgtcg tgatggcctg
      421 ctcgtcggcg gcgtattgcc aggacggcca ggcgaccagg gccaggaagg ccaggcaggc
      481 gccggtggcc acggcctcca gcatcgcgcg gcgcgattcg ctgcagcggc caaccagcgc
      541 cgtcatgcgc atgtgctcgc cgcggcgcag cgccacgacg gcgcccagca tcgccagcca
      601 caagaagagg atggaggcca gctcgtcgct ccacaccagg ggctggcgca agacgaagcg
      661 cgcgaacacc cccgagaaca gcaccgcgac atcggccagc acgagcagcg cggccggcca
      721 ttcgacgagc gggcccagca gggcgtcgag gcgctcgagc ttcgtcggcg cagccgcgac
      781 ctgcgcctgg ccgacgccgg ccggatcggc ggcggcggcc ttcacccgag cttgccggtg
      841 ctgcgctcga gcagcgccca ggcttcatcg ccgaacttgg ccttccattc ggcatagaag
      901 cccgccttgc gcatgcgctc gcggaagctg tcggtcttgg gctggttgaa caccatgccc
      961 ttggcggcca gatccttctg cagcgaggcg ttcagcgccg cgacatcggc gcgctccttc
     1021 aggccggcgg cgttgatgtg cctggcgacg atggcgcgca agtcctcagg caggcgctcc
     1081 caggccttgc ggttggcgag gaaccagaag ccgtcccaca tgtggttggt cagcgagcag
     1141 tacttctgca cttcgtagag cttggccgtg gcgatgaccg ccagcgggtt ctcctgcccg
     1201 tcgaccacct tggtctgcag ggccgaataa acctcggcga aattgatcga cgcgggcgcg
     1261 gcgtccagcg ccttgaacat cgaggtccac agcggcgagg gcggcacgcg cagtttcatg
     1321 cccttgaggt cgtcggccgt ctgcaccgga tgggtcgagg tcgtgatctg gcggaagccg
     1381 ttgtcccaga tcttgtccat cgccaccagg ccggccttgg cgatctgcgc gcgcacatag
     1441 gcaccgagct cgccgtccag cgccttccac accgtgtcgt agtcggggaa ggcgaagccg
     1501 atgccgctga tagcggcggc cggcaccagc gtcgcgagaa tgaggccgga cagcgtgaag
     1561 aactcgatgc cgcccgagcg tacttggctg agcatgtcgg tgtcgccgcc gagctgattg
     1621 ttggggaaga tctggatgtc gacgcggccc ttggtgtctg cgcggatggc atcgaccatc
     1681 tccttggcgc gcagattcat cgggtggctg accggcaggt tgttggcgta cttgtagctg
     1741 aactccgccc tctgggcttg ggcgcggccg gtggacaggg ccagcgcccc ggcaccggcg
     1801 agaagactgc gtcgcgtgag gctcatggtt cgtctccgtt cttgtttaag gtttaagggg
     1861 ctgtgccgat tctggcaaag cgcggcccag cgcagaatgc ggctttcttc ttagtcagtt
     1921 aagcagggaa gcctttcatg acgcagcaag cctcatctac ccccgtggcg gtagtcaccg
     1981 gcggcgcgcg cggcatcggc ctggccgtcg cccgctggtt cctcgcgcat gagcaccgcg
     2041 tcgcgctgct ggacatcgat gccgacaccc tgggccgcac cgaggccgcg ctcgacgatc
     2101 cgcagcgcgt gctggccctc cattgcgacg tgtcgcagcc agggcaggtg caggccgccg
     2161 tcgctgcggt ggcgcagcgc tatggccgca tcgacgcgct ggtcaacaac gccggcgtgg
     2221 ccgtgttcaa gcccatcgcc gacacctcct acgaggaatg gcgccatgtg ctgggcacca
     2281 atctcgacgg cgccttcctg tgcacccagg cctgcgcccc gctgatgcgc gccgggggcg
     2341 gcggcagcat tgtcaacatc gcgtcgatct ccggcctgcg cgccagcacg ctgcgcgtgg
     2401 cctacggcac cagcaaggct gcgctgatcc atctcaccaa gcagcaggcc gtggagctgg
     2461 gcaatgtcgg catccgcgtg aacgtcatag cgccgggccc ggtcgacact gagatggcca
     2521 agctggtgca tagcgtcgcg atacgctccg actactacga cgccatcccg ctgaaccgct
     2581 acggcacgcc ggaagagatc gccgaagccg tgggcttcct ctgcagcccg gcggccagct
     2641 atgtcaacgg ccagctgctg gcggtggacg gcggcttcga cgcctgcggc gtcgggctgc
     2701 cgacgctgcg caagaatgcc gccgcaagct gaagaaggac accatgagct acgaccccta
     2761 cgaccacgac ctcgaacgcg gcatgcgcaa ccggcgcggc atcctgggcg atgcctgggt
     2821 ggacaaatcg gtggccaatg ccaatagctt caatgcagag ttccagaact tcatcacgcg
     2881 ctacgcctgg cacgagatct ggggccggcc cgggctggac cccaagacgc gccgcatcat
     2941 cgtgctgtcc atcacctgcg ccctgggtcg ctgggaggaa tacgagctgc atttgcgcgc
     3001 cgccttgtcg gccggtgaag acacgcgcct gacacccgac gacgtgaagg aagtgctgat
     3061 gcaggccgcc atctatgccg gcgtgcctgc ggccaacacc ggcgtggcga tcgcggtgcg
     3121 gctgatgcgc gagctgggca tggagctgcc gccgctgccg gccaccgagg tctcccaccc
     3181 cggcgtcggc cgcgagggcc gcactgcctc gcgaccggcg ctgcattaca cactgcgcga
     3241 gccgcgcaac gggcaagcgc cccggcacac catcgtgctg gcccacgccc tgggctgcga
     3301 cctgatgatg tgggacgcct tggcgaacac gcttgcggcc gactgccgcg tcatcagctt
     3361 cgatgcgcgc ggccatggcg gctccgacgc gccggccggc ccctacacga tggccgagct
     3421 ggccgaggat gccgagcgcc tgctcgacga gctcgacgcc gaccctgtcg tctggctggg
     3481 cctgtcgatg ggcggcatgc tgggccagga actggcgctg cgccatcctc agcgcctgaa
     3541 ggccctggtg atcgccaact cgacctcagg ctatccaccc gagggccggg ccatctggga
     3601 ccagcgcatc gcagcgatcg aggccggcgg tctggaaacc gtggtcgacg gcaccatgca
     3661 gcgctggttc caccagggct ttcacgccgc ccagccggcc acggtggcac gctggcgccg
     3721 gcgcgtcgtg acgaccgacg agcagggcta tctcgccgcc tgcgccgccg tcaaggcgca
     3781 tgacacgacg gcgcggctgc cgcggatcaa gctgccgacg ctgatcatcg ccggcgagct
     3841 ggaccagggc acgccggtcg cgatggccga gctgatggcc aggcagattc ccggttcgcg
     3901 cctcgtcgtg ctgaaggagg cttcgcacct gagcgctctg gagcagccag aggccttcga
     3961 ggcggccgtg cgggacttcc tcgcgacgct ctagcttgct cgtcggctgc gctcaggcga
     4021 acggcgacgg ctcgaagtct ctggccagcg cccgccagat gcgctcggac gtgaccggca
     4081 tctggatgcg ctcggcatcg gcccccaggc cggcgtggtc cagcgcatcg acgatcgcgt
     4141 tcacgaccgc cggcgtcgcg cctatcgtgc ccagctcgcc gacgcccttc acgcccaagg
     4201 ggttggtctt gcagggaatc gacgtgtcga actcggtctt gaagccgcgg aaggtatcga
     4261 cgcgcggcaa ggcgtagtcc atgaagctgg cgctgagcag atggccgctg cccgcgtcgt
     4321 agcgcactct ctcacacagt gcctggccta tgccctgcat cgcaccgcct tcgatctgtc
     4381 cgcgcacgat gacggggctc acgacgcggc cgatgtcgtt gaccgaggca taggccacga
     4441 tctcgacctc gccagtgtcc ggggcaatct ccacctcgca gacatggcag gcattgggcc
     4501 agctcggccc gctcaccgag ctggtggaat cgacgtggat cctcgcctcg ctctgacggc
     4561 cggccagctc gaagaggtcg atgcccagat cggtgcccgc gacgcggaag cggccggcgc
     4621 ggtattcgat gtcggccgcc ggcgcttcca gcgcctgcgc cgccaggtcc ttcgcatgca
     4681 cgatggtctt ctgcgatgcg atgctcaccg aggagccgcc ggtgaagagc gagcgcgagc
     4741 ccgcgctgcc gaagccattg gcgcgatcgg tgtcgccctg cacgatgcgg atgcgctcga
     4801 tcggcacgcc gaagacgtcg accgccagct gcgcatagct ggtctggatg ccctggccca
     4861 tctgctgcgt ggccgagacg atctcgatga aaccatcggg cgtcaccgtg acctcgacgc
     4921 gctcctcgaa ggcattgccg ccggtccatt cgaggaaggt cgcgatgccg cggccgcgca
     4981 acttgccctt gcgctttgag gcttcgcgcc gcgtggcata gccgtcccag tcggccagct
     5041 tcaggccctg gtcgaggatc ttctcgaagg ccccgctgtc ataggtctgc cccatcgcgt
     5101 tcttgtaggg catctgctcg ggcttgatca tgttgcggcg gcgcagctcg gcggggttga
     5161 ggcccaactt gcgcgccgcg gcatccatca gccgctcgat gagatagatc gcctcgggcc
     5221 ggcctgcgcc gcggtagggt ccggtggggg cgcggttgct catcacggca ctgaagtgca
     5281 ggtcgatgac cgggatgtcg tagatgctgg tcgacaccca gggtccgatc agcagctgga
     5341 tggccacgcc ggcgccggtc gcataggcgc ccacattggc ataggagcgc acgcgcagcg
     5401 cgagcacgcg gccgctggcg tccaacgcca gctcggcgcg gctgagcaca tcgcggccgt
     5461 gcaatgcgga gacgaattcc tccaggcgct cggcctgcca cttgaccggg cgcttgagct
     5521 gcagcgccgc gtagccgacg gcaatgtcct cgggataggg cccggtcttc atgccgaagc
     5581 cgccgccgac atcgccgacc aacacgcgca ccttctcggg tgtcaggccg ggaatggtgt
     5641 cgcacaggcc gccgcgcacc gcggtgggca tctggctgga caaacgcatc gtcaaccggc
     5701 cggcgtcgaa ggcggccagc acgctgcgcg gctccatcgg gctcggcgcg aggcgctggt
     5761 tgtcgatgtc cagcatcacc ctgtgcgcgg cagaggccaa ggccgcttcg gccgccctgg
     5821 cgtcgccatg gcgcatttcg gcggcgatgt tgtccggcgc ctgcgcgcac agcaccggcg
     5881 cgccgggctg catcgcggcc atggtgtcga cgaccgccgg cagctcctcg tagtcgaccc
     5941 acagcgcatc ggccgcggcg cgcgcggcct cgcggctgtc ggccaccacc gcggccaccg
     6001 cctcgccgac gaagcgcacc acctcgtgcg ccagcgcgcg gcgcggtgcc gtcgcgccgg
     6061 ggctgccgtc ggcgcgcgcg aaaccggggc cggccatcgg cttgacgccg gccttgacga
     6121 gctgctcgcc ggtgaacacg gcgagcacgc cgggcatgcc cgcggccgtc gacacgtcga
     6181 tcgacttgat gcgcgcatgc gcatacggcg agcgcaggaa ggcgaggtgc gtctgcccgg
     6241 gcagcgagac gtcgtcggcg tactggccgc ggccggcgac cagcgccggg tcttcgacgc
     6301 ggcgcaccgc gtggccgctg ccgtagcggc cggcttcgat gggcgtcgtc atgccttggc
     6361 tccttgcatc gcggccgccg cctgcttcac cgcggcgacg atgttgacgt agcctgtgca
     6421 gcggcacagg ttgccttcca gcgcctgcac gatctcggct tcgctcgggt tcgcgttgtg
     6481 ctgcagcagg
//
LOCUS       DASUCY010000291         4633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_230945, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000291 DASUCY010000000
VERSION     DASUCY010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4633
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230945"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VFV25_05610"
     CDS             <1..288
                     /locus_tag="VFV25_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY1392828.1"
                     /translation="DTMDMGEQGKYYILKSADGKGRAGLAQASDPKAPAAWMPYVHVA
                     DCDGSVAQAGKLGAQTTILPPYDIPNVGRIAIFLDSLGAPIGVIKAAPMAS"
     gene            complement(337..1449)
                     /locus_tag="VFV25_05615"
     CDS             complement(337..1449)
                     /locus_tag="VFV25_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392829.1"
                     /translation="MTATLPAAPVKTPAGQDELRRRTRGLGQRFRTVLLLVDGQRPLS
                     EVLSLALQAGAATSHFEELLRLGLVELPGQAESGDDDELEPMPSLESQETAPGELGQP
                     HVKDVELAVPAAAEAAHGLGTMAPLLQHDEQEAEAEPVAAVFEEEAAVVRETAPREVR
                     PAPSLHQAEPALAAEPAGDAVGEYPPFPDTVGVLESAEEETSEPAAAASAAGDDGLLL
                     NPRLMEQAALYRPSKDDLPPWMAASQAAEEPATKTTPARAGKPAASAPAAPVRRPARP
                     LNEDVRHDPFLMQVRGLLLDALHLDPPPFGSRIYSRVSGATTIAELVEQVWEIERYMS
                     RGRRSRGGMSALLQARDMLGLGNTMVPEDSRIDEDE"
     gene            complement(1624..2160)
                     /locus_tag="VFV25_05620"
     CDS             complement(1624..2160)
                     /locus_tag="VFV25_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004549259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3761 domain-containing protein"
                     /protein_id="HEY1392830.1"
                     /translation="MTIKQLMTSVACAAGLFASAGAFAAAPADAPAGTTGMCKDGSYS
                     SAAEKKGACRGHKGVKEWYSDAAPAAAAAKTTKAAAPAAAATPAATTAPAAAAATTAP
                     AAATAAAPATKTAAAKPAAGAAPGGGAGQVWANDTTKVYHCEGDKWYGKTKHGEYMSE
                     ADAKAKGFHADHGKACSK"
     gene            2434..2871
                     /locus_tag="VFV25_05625"
     CDS             2434..2871
                     /locus_tag="VFV25_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392831.1"
                     /translation="MRLARWLIALAVPIALVILYFFAVLHWSYSSGERAGWVQKLSRK
                     GWICKTWEGELALVSLPGSTPEKFDFTISDDAVAEQLNRVMGKRVALHYEQKVGLPTS
                     CFGETRYYVTGVRVQDEIPLSAGVVVPGQAAAAGATPAASAPR"
     gene            complement(2843..4222)
                     /locus_tag="VFV25_05630"
     CDS             complement(2843..4222)
                     /locus_tag="VFV25_05630"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HEY1392832.1"
                     /translation="MMRSITTRLVLMFALAALATFGLIGAALYGVLRGELARHEEEQL
                     NTNLQNLQYSMERIDSVERWARVQAKMDTLTPADGSVRFWVLSDDARFQYGTGLAEFE
                     HLSLDADGRGRIVMQDHELHLRASSVAALGDRPAVRLIVGVDAAPYAHTLRAFLSALA
                     VLALSAMLLVMLAGYWIARLGLRPLQRLSQEARALSPKTLSQRLQLAQLPVELSDLAG
                     AFNGALGRLEKAYQQLEAFNADVAHELRTPLANLIGGTQVALARQRSASEFEEVLQSN
                     LEELERLRSIVNDMLFLARADRGEAATGLVSTPVAEEVRKTIDFFEFVLDDMQASVSI
                     AGDVDAEAPIERALFRRAVSNLLQNAIEHSAKGARLTVKISQQLTAIWINVSNPGAAI
                     AEAHLPRLFDRFYRVDAARQGQGGHHGYGLGLAIVKAVASMHGGSVAASSADGITTIG
                     FSVALRPQA"
     gene            complement(4219..>4633)
                     /locus_tag="VFV25_05635"
     CDS             complement(4219..>4633)
                     /locus_tag="VFV25_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY1392833.1"
                     /translation="DGADDYLVKPFSFLELLARLQALRRRGRSQEPTQLRIGDLQIDL
                     IARKAWRGATRIDLTAKEFALLAVLARRQGEILSKTAIAELVWDMNFDSNTNVVEVAI
                     KRLRDKIDAAFAPKLLHTVRGMGYVLEPREQEAAP"
BASE COUNT          668 a         1599 c         1618 g          748 t
ORIGIN      
        1 gacacgatgg acatgggcga gcagggcaag tactacatcc tcaagagcgc cgacggcaag
       61 ggccgcgccg gcctggccca ggcctcggac cccaaggccc ccgcggcctg gatgccttat
      121 gtgcacgtgg ccgattgcga cggcagcgtc gcccaggccg gcaagctggg cgcccagacc
      181 acgatattgc cgccgtacga catccccaac gtgggccgca tcgcgatctt cctcgattcg
      241 ctgggcgcgc ccatcggcgt catcaaggca gcgccgatgg caagctgatc tctcgtgcgc
      301 cggcgggggc tcggcccccg cggcgcaagt cgggactcac tcgtcttcgt cgatgcgcga
      361 atcctcgggg accatggtgt tgcccaggcc cagcatgtcg cgggcctgca gcagcgccga
      421 catgccgccg cgcgagcgcc ggccgcggct catgtagcgc tcgatctccc agacctgctc
      481 gaccagctcg gcgatggtgg tcgcgccgct cacgcgcgag tagatgcgcg agccgaaggg
      541 cggcgggtcc agatgcagcg cgtccagcag caagccgcgc acctgcatca ggaaggggtc
      601 gtggcggacg tcctcgttca gcggccgggc gggcctgcgg accggagcag caggcgcgga
      661 cgccgccggc ttgcccgcgc gagcgggcgt cgtcttggtg gcgggctcct cggcggcctg
      721 ggaggcggcc atccagggcg gcaggtcatc cttcgagggc cgatacaggg ccgcctgctc
      781 catcagcctg gggttgagca gcaacccatc atcccccgct gcgctcgccg ctgcagcagg
      841 ctcggacgtc tcttcttccg cggactccag gacgcccacc gtgtcgggga agggcgggta
      901 ttcgccaacg gcgtcgccgg cgggctctgc agcaagcgcc ggctctgcct ggtgcaagga
      961 cggagctggg cgaacttcgc gcggcgcggt ttcgcgaacc accgcggcct cttcctcgaa
     1021 gacggcggcg actggttctg cctcggcttc ctgctcatca tgctgcagca gcggagccat
     1081 ggtcccgagc ccatgcgccg cctcggctgc agcgggcacc gccagctcca cgtccttcac
     1141 atgaggctgt cccagctcgc cgggcgcagt ctcctgcgac tccagggacg gcataggctc
     1201 cagctcgtca tcgtctccgg actcggcctg ccccggcagt tccaccaggc ccaggcgcag
     1261 caattcctcg aagtggctgg tggcggcgcc ggcctgcagc gccaggctca agacctcgct
     1321 cagcggccgt tggccgtcca ccagcagcag caccgtgcgg aagcgctgcc ccagcccgcg
     1381 cgtgcgacgg cgcaactcgt cctgcccagc gggcgtcttg acgggagcgg cggggagggt
     1441 cgcagtcacc ttggatttct ggaaccgtat tgccgcgcat tgtcgcgcgg ctcgtgcaag
     1501 ttttgtgact cgcgcaaggt gagcgaatgt agcggcgtgt cccatgaaaa agcgccggcg
     1561 tgcggggcac gatccggcgc ttctgcgagt ggcgggcgcc aggcccgcca tcatgcttcg
     1621 gccttacttg ctgcaggcct tgccgtggtc ggcgtggaag cccttggcct tggcgtcggc
     1681 ctcggacatg tactcgccgt gcttggtctt gccgtaccac ttgtcgcctt cgcagtggta
     1741 gaccttggta gtgtcgttgg cccagacttg gccggcgccg ccgccgggag cagcgccggc
     1801 agccggcttg gcggcagcgg tcttggtggc cggagcggcg gcggtggcag cagcgggagc
     1861 cgtggtcgcg gcggccgcgg ccggagccgt ggtagcagca ggggtggcgg cggcagccgg
     1921 agcggcggcc ttggtggtct tggctgcagc agcggccggc gcggcgtcgg agtaccactc
     1981 cttcacgccc ttgtggccgc ggcaggcgcc cttcttctcg gcggcggacg aatagctgcc
     2041 gtccttgcac atgccggtcg tgccggccgg ggcatccgcg ggggccgcgg cgaaggcgcc
     2101 ggcggaggcg aacaggccgg cagcgcaggc cacggaagtc atcagctgtt tgatcgtcat
     2161 ctggctttcc ttcaagcggt gattagctcc atgtcggagc acgccttcct ccaacgcgcg
     2221 gcagccgagg tcgttgacgg cagccaggcg catcggcttc ggtcccgcgg cggatcggag
     2281 ttcttttggg cgccgccgcg gggtcgggcc gcatgctccc acgctgatgt gacagccgac
     2341 ctgacacgga gctgagagcg gcctggctct acactgcgca ccatcgccat gacaaacgag
     2401 ccggcagcgc cggcctttcg gaggaatacc cccatgcgcc ttgcccgctg gctgatcgcc
     2461 ctggccgtcc ctatcgcgct cgtgatcttg tatttcttcg ccgtcctgca ctggagctac
     2521 tcctcgggcg agcgcgccgg ctgggtgcag aagctgtcga gaaagggctg gatctgcaag
     2581 acctgggaag gcgagctggc gctggtctcg ctgcccggca gcacgcccga gaagttcgac
     2641 ttcacgatca gcgacgatgc ggtggccgag cagctcaacc gcgtgatggg caagcgcgtc
     2701 gcgctgcact acgagcagaa ggtgggcctg ccgaccagct gcttcggcga gacccgctac
     2761 tacgtgaccg gcgtgcgcgt gcaggacgag atcccgctgt ccgccggcgt cgtggtgccg
     2821 gggcaggcag ccgctgctgg agctacgcct gcggcctcag cgccacgctg aagcctatgg
     2881 tggtgatgcc gtcggcgctg ctggcggcca cactgccgcc atgcatgctg gccaccgcct
     2941 tgacgatggc caggcccagg ccatagccgt ggtgtccgcc ctggccttgg cgcgccgcgt
     3001 cgacgcggta gaagcgatcg aacaggcgcg gcagatgggc ctcggcaatc gccgcgcccg
     3061 ggttcgagac attgatccag atcgccgtca attgctgaga gattttcact gtcagccggg
     3121 ctcctttggc cgaatgttcg atcgcattct gtagcagatt cgagaccgcg cggcggaaca
     3181 gcgcgcgctc gatcggcgcc tcggcgtcga catcgccggc gatgctgacg ctggcctgca
     3241 tgtcgtccag cacgaactcg aagaagtcga tggtcttcct gacctcctcg gcaactggcg
     3301 tgctcaccag gccggtggcc gcctcgccgc ggtcggcgcg cgccaggaag agcatgtcgt
     3361 tgacgatgga acgcagccgc tccagctcct ccaggttgga ctgcagcacc tcctcgaact
     3421 cgctcgcgct gcgctggcgc gccagggcga cctgggtgcc gccgatcagg ttggccagcg
     3481 gcgtacgcag ctcgtgggcc acatcggcat tgaaggcttc gagctgctga taggcctttt
     3541 ccaggcggcc cagcgcgccg ttgaaggcgc cggccaggtc cgagagctct accggcagct
     3601 gcgccagctg cagccgctgc gacagcgtct tcgggctcag tgcgcgcgcc tcctgcgaca
     3661 gccgctgcag cggccgcagg cctagcctgg cgatccaata gccggccagc atcacgagca
     3721 gcatggcgct caaggccagc acggccagcg cgctcaggaa ggcgcgcagc gtgtgcgcat
     3781 agggcgccgc atcgacgccg acgatgagcc gcactgccgg gcggtcccct agcgccgcga
     3841 cgctgctggc gcgcaggtgc agctcgtggt cttgcatcac aatgcggcca cggccgtcgg
     3901 catcgaggct caggtgctcg aactcggcta ggccagtgcc gtactggaag cgcgcatcgt
     3961 cgctcagcac ccagaagcgc acgctgccgt cggccggcgt cagcgtgtcc atcttggcct
     4021 gcacgcgggc ccagcgttcg acgctgtcga tgcgctccat cgaatactgc aggttctgca
     4081 gattggtgtt gagctgctct tcctcgtggc gcgccagctc gccgcgcagc acgccgtaca
     4141 gcgcggcgcc gatcagcccg aaggtcgcca gcgcggccag cgcgaacatc aacaccagcc
     4201 gcgtcgtgat cgaacgcatc atggcgccgc ttcctgctcg cgcggctcca gcacatagcc
     4261 catgccacgc acggtgtgca gcagcttggg cgcgaaggcc gcgtcgatct tgtcgcgcag
     4321 ccgcttgatc gcgacctcca cgacattggt gttgctgtcg aagttcatgt cccagaccag
     4381 ctcggcgatc gccgtcttgg acaggatctc gccctggcgc cgcgccagca cggccagcag
     4441 cgcgaattcc ttggccgtga ggtcgatgcg cgtggcgccg cgccaggcct tgcgggcgat
     4501 gaggtcgatc tgcaggtcgc cgatgcgcag ctgggtcggc tcctggctgc gcccgcggcg
     4561 tcgcagggcc tgcagccgag ccagcagttc caggaaggag aagggcttga cgaggtagtc
     4621 gtccgcgccg tcg
//
LOCUS       DASUCY010000292         6948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1259158, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000292 DASUCY010000000
VERSION     DASUCY010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 6948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6948
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1259158"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..1154)
                     /locus_tag="VFV25_05640"
     CDS             complement(108..1154)
                     /locus_tag="VFV25_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEY1392834.1"
                     /translation="MKPQIYTRAAQLPELLQQRILVLDGAMGTMIQRYKLGEADYRGE
                     RFKDHPKDLKGNNELLQFTRPDVIREIHEGYLAAGADLIETNTFGATSVAQEDYGLAE
                     YAREMNVAAARLARAAADKFSTQDKPRFVAGAFGPTPRTASISPDVNDPGARNVDFDT
                     LKAAYYEQAAGLLEGGADVFLVETIFDTLNAKAAIFAIDELFEASGERLPVIISGTVT
                     DASGRILSGQTVSAFWHSVRHAQPLAVGLNCALGAALMRPYIEELSKAAGDSFISCYP
                     NAGLPNPMSETGFDETPEVTGGLMEDFAKAGFLNLVGGCCGTTPEHIAAIAKRVSAYR
                     PRGRQGLFTELNAA"
     gene            complement(1213..3507)
                     /locus_tag="VFV25_05645"
     CDS             complement(1213..3507)
                     /locus_tag="VFV25_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY1392835.1"
                     /translation="MVKRHQSGHLLLSPGLTEAPVLDRMCSHFVLTLTLKSAGHFNLR
                     RDWNGLLSLTGRHLVWPASVLARLREFLARRVRGNPQWAGHEELSDAAFVARFGAWRG
                     PYEEGTLFFYIDEYIKDQAKDLMVALGATCEWLERDLKKASTLVEKNIDALAGLLQLN
                     PAERALLLYGTLARYQRDLRGLLVEFKVSNAQEAFAALAEVAGVDEKDVAEALRAGSR
                     LERIGMVENLISEHNITDLADLMKVSEQLPPVLMREYRDQSELMAVFTRPASPTELAM
                     SDFAFVAEDARVLASLLTNAVVHKEAGVNILLYGPPGTGKTELAKVVAQAAGLELYEV
                     EYADRDGNSLSGRDRYRSLQISQVFLKGSHKVALLFDEVEDVFPPISTDTAQLMARLD
                     SGEAGVSGSVSGKAWVNQILETNPVPVIWVTNRIEQIDPAFRRRFQYHLELTSPPPGA
                     REAIVARALESVAATPEFVSKLASRQGLTPAQIRTAVRFAKLAHSEGAEAQALIERQL
                     ANADKALGLQSRRAARAVVTRYDLSLLNIESRFEVPRIVEALRRRGSGTLCFHGPPGT
                     GKTALAEHLAAALQRPLLVRQASDVMSKYVGETEQNMARMFEEAEREGGVLLLDEADS
                     FLRSRRMAERNYEVSEVNEMLQGMERYAGIFVCTTNLFEELDEAALRRFTFKIRFKPL
                     TVEQRERMFVAEALGGDAAPDAEQRARLQAMDLLTPGDFAAVRRQAEILAAALTPEEF
                     LAQLEAEHRVKPEVRQRRGIGFLH"
     gene            complement(3640..3921)
                     /locus_tag="VFV25_05650"
     CDS             complement(3640..3921)
                     /locus_tag="VFV25_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003264554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3567 domain-containing protein"
                     /protein_id="HEY1392836.1"
                     /translation="MQMLYNSDNFAVVQFDVPASAGAGEHGAPAGLMRGGYEIVDKFA
                     RKEIFLEGALAESFKEGVEALIETSPGIEELDAFVERFANLAQQPVVLH"
     gene            complement(4093..5232)
                     /locus_tag="VFV25_05655"
     CDS             complement(4093..5232)
                     /locus_tag="VFV25_05655"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEY1392837.1"
                     /translation="MREPRRRLWRSLLPATLIALVLHAWLLKALARDDANPSPQPQAS
                     ALRVRQIHAEPSPQMMNDAIPPSLGKPEAALAAPAQRTAPPPARERVLSAEAGQPPAQ
                     PVPQAEPHEALATDLPAVGSDAAPGDAAPRDASSDRTMLASNEEAAAAVPNLAPAPAH
                     PPPVYPTRIPPTTTLSYELKRGFLSGSGELRWSNLGQRYQLSLEGRLPIIGTLLTQRS
                     SGGFDNAGLAPERFTEKRRGRSEQAANLQRSAGIVSFSGPSNEYPLLAGTQDRLSWMV
                     QMAAIAEAWKHVPPAGERLYCFIVGARGDGDVWVFSVEGQEVISTALGELRAVKLMRV
                     PREPHDTKVEVWLDPTHHHLPARVRLTENSGDPLELSLQATSPPP"
     gene            complement(5225..6073)
                     /locus_tag="VFV25_05660"
     CDS             complement(5225..6073)
                     /locus_tag="VFV25_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012840034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY1392838.1"
                     /translation="MPATRDFAATSDATPGDADERAGIQSVEVGYRLLEALASAHGAL
                     ALKDLAQAAGMNAAKAHRYLVSFQRLGLVVQEAATSHYDLGPTALRLGLAGLARVDAV
                     RLARQAAARLCTQINHSTAVAVWGNHGPTIVHSQESRQPVVASLRLADVMPLLSSATG
                     RCFCGWLPREQVLPLVRRELLQAVQQGRQDLPRTAEDFDALREAVRKRGAGEVAHTLL
                     PGISALCHPVFDADGHLALGLLSLGPSAIFDPRQGGEIDRALGRAARELSRELGHRHD
                     ASLAHA"
     gene            6275..>6948
                     /locus_tag="VFV25_05665"
     CDS             6275..>6948
                     /locus_tag="VFV25_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEY1392839.1"
                     /translation="MKLATYKDGSRDGQLVVVSRDLNSAHYASGIASRLQQVLDDWNF
                     LSPQLQDLYETLNAGKARHAFAFDPRLCMAPLPRAYQWADGSAYINHVELVRRARKAE
                     MPESFYTDPLMYQGGSDDFLGPCDDIVCASEDWGIDFESELAVITGDVTMGVSPEEAL
                     ESVRLLMLANDVSLRNLIPAELAKGFGFFQSKPATAFSPVAMTPDELGAAWQGGRVHL
                     ALQSDW"
BASE COUNT         1103 a         2326 c         2373 g         1146 t
ORIGIN      
        1 gtgcggccgc ccactgaggc gaggtgccgc cacgaatggt tcgctacgtt tttagcagcg
       61 cacttcgtta aatggcgccg agcaaccagt agaaaaccgc caaatcctca ggctgcattc
      121 agctcggtga acagcccctg ccgtccgcgc ggccggtagg ccgagacgcg cttggcgatg
      181 gcggcgatgt gctccggcgt cgtgccgcag cagccgccga cgaggttgag gaagccggcc
      241 ttggcgaagt cttccatcag gccgccggtg acctccggcg tctcgtcgaa gccggtttcg
      301 ctcatcgggt tgggcaggcc ggcattggga tagcaggaga tgaaggaatc gcccgcggcc
      361 ttgctcagct cctcgatata gggccgcatc agcgcggcac cgagcgcgca attgaggccg
      421 acggccagcg gctgggcgtg gcgcaccgaa tgccagaagg cgctgacggt ctggccggag
      481 aggatgcggc ccgaggcgtc ggtgaccgtg cccgagatga tgacgggcag gcgctcgccg
      541 cttgcctcga acagctcgtc gatcgcgaag atcgccgcct tggcattgag cgtgtcgaag
      601 atggtctcga ccaggaagac atcggcgccg ccttccagca gccccgcggc ctgctcgtaa
      661 taggcggcct tgagcgtgtc gaagtcgacg ttgcgcgcgc cggggtcgtt gacgtccggg
      721 ctgatgctgg cggtgcgcgg cgtggggccg aaggcgccgg ccacgaagcg tggcttgtcc
      781 tgcgtgctga acttgtcggc ggccgcgcgc gccagccgcg ccgcggcgac gttcatctcg
      841 cgggcgtact cggccaggcc atagtcctcc tgtgcgaccg aggtggcgcc gaaggtgttg
      901 gtctcgatga ggtcggcgcc ggctgcgaga tagccctcgt ggatctcgcg gatcacgtcg
      961 ggccgcgtga actgcagcag ctcgttgttg cccttcagat ccttgggatg atccttgaag
     1021 cgctcgccgc ggtagtcggc ctcgccgagc ttgtagcgct ggatcatcgt ccccatcgcg
     1081 ccgtcgagca cgaggatgcg ttgctggagc aattcgggca gctgcgcggc gcgcgtatag
     1141 atctggggct tcatgatggc cggattgtag aaagcccggc cacgccgcat gcagccaggg
     1201 tgaatgccga gctcagtgca ggaatccgat tccgcgtcgc tggcgcacct cgggcttcac
     1261 gcgatgctcg gcctccagct gagccaggaa ttcttccggc gtcagcgcgg ccgcgaggat
     1321 ctcggcctgg cggcgcacgg ctgcgaaatc gccgggggtc agcaggtcca tggcctgcag
     1381 gcgcgcgcgc tgctcggcgt ccggcgccgc atcgccgccc agcgcctcgg cgacgaacat
     1441 gcgctcgcgc tgctcgaccg tgaggggctt gaagcggatc ttgaacgtaa agcgccgcag
     1501 cgcggcctcg tccagctcct cgaacagatt ggtggtgcag acgaagatgc cggcatagcg
     1561 ctccatgccc tgcagcatct cgttgacctc gctgacctcg tagttgcgct cggccatgcg
     1621 gcggctgcgc aggaagctgt cggcttcgtc gagcagcagc acgccgcctt cgcgctcggc
     1681 ctcctcgaac atgcgcgcca tgttctgctc ggtctcgccg acgtatttgc tcatcacgtc
     1741 ggaggcctgg cgcaccaaca gcggccgctg cagcgctgcg gccagatgct cggccagggc
     1801 cgtcttgccg gtgcccggcg ggccgtggaa gcacagcgtg ccgctgccgc gccggcgcag
     1861 cgcctcgacg atgcgcggaa cctcgaagcg gctctcgata ttcagcaggc tcaggtcgta
     1921 gcgcgtcacc acggcccgcg cggcgcgccg gctctgcagg cccagcgcct tgtcggcatt
     1981 cgcgagctgg cgctcgatca aggcctgcgc ctcggcgccc tcgctgtgcg cgagcttggc
     2041 gaagcgcacc gcggtgcgga tctgcgccgg cgtcaggccc tggcggctgg ccagcttgct
     2101 gacgaactcc ggcgtggccg ccacgctctc gagcgcccgc gcgacgatgg cctcgcgcgc
     2161 gcccggcggc ggcgaagtga gctcgaggtg gtattggaag cggcggcgga aggcggggtc
     2221 gatctgctcg atgcgattgg tgacccagat caccggcacg gggttggtct cgaggatctg
     2281 gttgacccag gccttgccgc tgaccgaacc cgagacgccg gcctcgccgc tgtccagcct
     2341 cgccatcagc tgcgcggtgt cggtggagat cggtgggaag acgtcctcca cctcgtcgaa
     2401 cagcagcgcg accttgtggc tgcccttcag gaacacctgg ctgatctgca gcgagcgata
     2461 acggtcgcgg ccggacagcg aattgccgtc gcggtctgcg tactccacct cgtacagctc
     2521 cagacccgcg gcctgcgcca ccaccttggc cagctcggtc ttgccggtgc cgggcgggcc
     2581 atagagcagg atgttgacgc cggcctcctt gtggaccacc gcgttcgtca gcagcgatgc
     2641 gagcacgcgc gcgtcttcgg cgacgaaggc gaagtcgctc atcgccagct cggtcgggct
     2701 ggccggccgc gtgaacacgg ccatcagctc ggactggtcg cgatactcgc gcatcagcac
     2761 cggcggcagc tgctcgctga ccttcatcag gtcggcgaga tcggtgatgt tgtgctcgga
     2821 gatcaggttc tcgaccatgc cgatgcgctc caggcgcgag ccggcgcgca aggcctcggc
     2881 cacgtccttc tcgtcgacgc cggccacttc ggccagggcc gcgaaggctt cctgcgcgtt
     2941 gctgaccttg aactcgacca gcaggccgcg cagatcgcgc tgatagcgcg ccagcgtgcc
     3001 gtacagcagc agcgcgcgct cggcggggtt gagctgcaag agcccggcta gcgcgtcgat
     3061 gttcttctcg accagcgtcg atgccttctt gagatcgcgc tcgagccatt cgcaggtagc
     3121 gcccagggcg accatcaggt ccttggcctg gtccttgatg tattcgtcga tgtagaagaa
     3181 gagcgtgcct tcctcgtagg gcccgcgcca ggcgccgaaa cgcgcgacga aggcggcgtc
     3241 gctcagctcc tcgtggccgg cccactgcgg attgccgcgc acgcggcgcg ccaggaattc
     3301 gcgcagcctt gccagcaccg aggcgggcca caccagatgc cgcccagtga gcgacagcag
     3361 accgttccag tcgcgacgca gattgaaatg cccggcgctc ttgagcgtga gcgtcagcac
     3421 gaagtgcgag cacatgcgat ccagcaccgg cgcctcggtg aggccgggcg agagcagcag
     3481 atgtccactc tgatggcgct tgaccacgca gcacttcctt caatgaaagg ctgaggcgat
     3541 cttggctcag ggcagcgccc ggggcaagcc ccgggaattc acggtttttt ctatcggggt
     3601 gttactctgc gcccgcgggg cgggggcgca tgctcccgct caatgcagca cgaccggctg
     3661 ctgagccaga ttggcgaagc gctcgacaaa agcgtccagc tcttcgatgc cgggcgaagt
     3721 ctcgatcagg gcctcgacgc cttccttgaa gctctcggcc agcgcgcctt cgaggaagat
     3781 ttccttgcgc gcgaacttgt cgacgatctc gtagccgcca cgcatcagcc cggccggcgc
     3841 gccatgttcc ccagcaccgg cgctggccgg tacgtcgaac tgcacgacgg cgaagttgtc
     3901 cgagttgtag agcatttgca tggaggcttc ctctttgagg tctacctata gccttaaacg
     3961 cggctttcaa gagcctggat gactcagggt tcgcgcagtt cttcacgcaa atgccatgcc
     4021 aatcgctggt gatgtaggtc cggcgtcaca gcgggacccg gctcagcccc ctcgggggga
     4081 gcgccgaagg cgctacgggg gtgggctagt cgcttgcagc gacagctcca gcggatcacc
     4141 gctgttttcg gtcaggcgca cgcgcgccgg cagatgatgg tgggtcgggt cgagccagac
     4201 ctcgactttg gtgtcgtgcg gctcacgcgg cacgcgcatc agcttcacgg cgcgcagctc
     4261 gcccagggcc gtgctgatga cttcctggcc ctcgacgctg aagacccaca catcgccgtc
     4321 accgcgggca ccgacgatga agcaatacag gcgctcaccg gccggcggca catgcttcca
     4381 tgcttcggcg atcgcggcca tctgcaccat ccagctcagg cggtcctggg tgccggcgag
     4441 cagcgggtat tcattgctgg ggccggagaa gctcacgatg ccggcgctgc gctgcagatt
     4501 ggcggcctgc tccgagcggc cacgacgctt ttcggtgaag cgctcgggcg cgaggccggc
     4561 attgtcgaag ccgccgctgc tgcgctgggt cagcagcgtg ccgatgatgg gcagccgccc
     4621 ttccagcgac agctgatagc gctggcccag attgctccag cgcagctcgc cgctgccgct
     4681 caggaagccg cgcttgagct catagctcag cgtggtcgtt ggaggaatgc gggtcggata
     4741 gaccggcggc ggatgggccg gcgcaggcgc aaggttcggc accgccgcgg cggcctcctc
     4801 gttcgaggcc agcatcgtcc ggtccgagga agcgtctcgc ggcgcggcat cgcccggtgc
     4861 ggcatcgctg cccaccgctg ggaggtccgt cgcaagcgcc tcatgcggct cggcctgagg
     4921 cactggctgc gcaggaggct gtcccgcttc ggccgacagg actcgctcgc gcgccggcgg
     4981 tggcgctgtg cgctgtgcgg gtgcggcgag ggccgcttcg ggcttgccta gcgacggggg
     5041 aatcgcatcg ttcatcatct ggggcgaagg ctcggcatga atctggcgaa cgcgcagcgc
     5101 cgaggcttgg ggttggggtg acggattggc gtcgtctctc gctagcgcct tgagcagcca
     5161 ggcgtgcaag acaagcgcga tcagcgtggc cggcagcagc gaccgccaaa ggcgacgcct
     5221 tggttcacgc atgggccagg ctcgcatcgt gacggtggcc cagctccctc gaaagctcac
     5281 gggccgcgcg gccgagggcg cggtcgatct cgccgccttg ccgtggatcg aagatggccg
     5341 aaggccccag gctcagcagg cccagcgcta ggtggccatc ggcatcgaat accgggtggc
     5401 acagcgccga gatgccgggc agcaaggtat gcgccacctc gccggcgccg cgcttgcgca
     5461 ccgcctcgcg caaggcatcg aagtcctccg ccgtgcgcgg cagatcctgg cgcccctgct
     5521 gcaccgcctg cagcagctcg cgccgcacca gcggcagcac ttgctcacga ggcagccagc
     5581 cacagaagca gcggccggtg gctgaactca gaagcggcat cacatcagcc aaacgcagac
     5641 tggccaccac cggctgacgc gattcctgac tatgcacgat ggtcgggcca tggttgcccc
     5701 agaccgccac cgccgtgctg tggttgatct gcgtgcacag ccgggcggcg gcctgacgtg
     5761 ccagccttac cgcatccacc cgggccaggc ccgccaagcc cagccgcagg gccgttggcc
     5821 ccaggtcgta atggctggtc gcagcttcct gcaccactag gcccaggcgc tgaaagctca
     5881 ccagatagcg atgcgccttg gccgcgttca tgccggcggc ctgcgccaga tccttcagcg
     5941 ccagcgcacc gtgggccgac gccagggcct cgagcaggcg atagcccact tccaccgact
     6001 ggatgccggc gcgctcgtcg gcatcgccgg gggtcgcgtc agacgtcgca gcgaagtctc
     6061 tggttgcagg catggcgcga tcgattatgc gaggccgcca gcgcgcttgg catgaagcag
     6121 tgattacgtt ttgtataaca aatttatcat atcgtaagaa cctgggttac caagagcgtc
     6181 tgcaatggcg cgcggcaggt cacaatctcg gctccctgaa gccgcagcgc aacgaccgcc
     6241 cggagcggcg gtgactcgaa ctaataaagc ccagatgaaa ctcgccacct acaaggatgg
     6301 ttcccgcgac ggccagctgg ttgtcgtttc gcgcgacctg aacagcgccc attacgccag
     6361 cggcatcgca tcccggctgc agcaggtgct ggacgactgg aacttcctgt cgccccaact
     6421 gcaggacctc tacgagacac tgaatgccgg caaggcgcgc catgccttcg ccttcgatcc
     6481 acggctgtgc atggcgccgc tgccgcgcgc ctatcagtgg gccgacggtt cggcctacat
     6541 caaccacgtc gagctggtgc ggcgcgcccg caaggccgag atgccggaga gcttctatac
     6601 cgacccgctg atgtaccagg gcggcagcga cgacttcctc ggcccttgcg acgacatcgt
     6661 ctgcgcgagc gaggactggg gcatcgactt cgaaagcgag ctcgccgtga tcaccggcga
     6721 cgtgaccatg ggcgtgtcgc cggaggaggc gctggaaagc gtgcgcctgc tgatgctggc
     6781 caacgacgtg tcgctgcgca atctgatccc cgccgagctg gccaagggct tcggcttctt
     6841 ccagagcaag ccggccaccg ccttcagccc ggtggcgatg acaccggacg aactcggcgc
     6901 cgcctggcag ggcgggcggg tacatctcgc actgcagagc gactggaa
//
LOCUS       DASUCY010000293         4834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_664906, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000293 DASUCY010000000
VERSION     DASUCY010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664906"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VFV25_05670"
     CDS             complement(<1..314)
                     /locus_tag="VFV25_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HEY1392840.1"
                     /translation="MSQDLNIINLVLHASFIVQIVMAGLLITSLVSWAVIFGKLFGLR
                     RVRGNNEEFEREFWSGRQLQELYSDAAKVASDNQRAAPMERIFASGMREFFKLRERRV
                     NEA"
     gene            complement(311..727)
                     /gene="ybgC"
                     /locus_tag="VFV25_05675"
     CDS             complement(311..727)
                     /gene="ybgC"
                     /locus_tag="VFV25_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HEY1392841.1"
                     /translation="MTETETVTDDYRFTLRVYWEDTDAGGVVFYANYLKFFERARTEW
                     LRSLGFAQEKLRTETGTMFVVTDTALRYRQPARLDDLLTVSVRLTERGRASLVLQQQA
                     LRGDTLLAEGTIRIGCVDTASFRPSRIPPEILNAIG"
     gene            complement(768..1193)
                     /locus_tag="VFV25_05680"
     CDS             complement(768..1193)
                     /locus_tag="VFV25_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TssQ family T6SS-associated lipoprotein"
                     /protein_id="HEY1392842.1"
                     /translation="MRLAGRSRLVLASLALFSFGLAGCCVQPPTAAPVGVMDLYQRPA
                     EKALLGGMRSYDDGQYAEAEKSFNAALGAGLASTKDRAAANKYLAFIYCTSRRTVQCE
                     AAFRAARAADPGFALSKSEAGHPIWGPVYRRTLTPLPAQ"
     gene            complement(1190..2812)
                     /locus_tag="VFV25_05685"
     CDS             complement(1190..2812)
                     /locus_tag="VFV25_05685"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY1392843.1"
                     /translation="MERPSNSPRNDLPSLPPGDSEPAPSTEPAAEGWDEADTVLLERT
                     LPPEVGLNVAADAPPEALPTIGHIGRYALKYRIGEGGLGMVYAAHDPLLSRPIAIKMV
                     NVQLSSTEAREQFNSLFLNEARAAGGLSHPHIVTVFDAGTSEQGAYIAMELLKGKDLR
                     ELLKEGWRPTPAQAALIVRRAADALAYAHTKGVVHRDIKPANIFMVGRTQPRLLDFGI
                     ARVTHQRDAVDLVAGSPYYMAPEQMREEPTDRRTDVFSLGVVLYELLTGKRPFTGNSL
                     VEISNAVQNREPPRAHTVDTRVPQGLSDIAARAMEKEPEHRYRSARALARDLRSWLDE
                     HPESLQANENEAPQRRKRLLFGAAAAAGLALIGFGLWRGGEEHATEQAAAPAASAATK
                     VAVATTTAAPAAPAPAASASPAPTPNGAAPIEAEPTAVPAGETVLAAQPANAQRQERA
                     AKAQREAHARTAKATAAAPGAAGVLHIAVSPWGDVEVDGAPAGTTPPLNRLTLSEGRH
                     TITIRNGDFPPYTATVVVSAGQPATLKYKFGS"
     gene            complement(2897..4084)
                     /locus_tag="VFV25_05690"
     CDS             complement(2897..4084)
                     /locus_tag="VFV25_05690"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY1392844.1"
                     /translation="MNLAARLAHIEPFYVMECAKAAAEIAASPACDPRQGGEPMIFLN
                     IGEPDFTAPPSVVEAAQRCLRAGRTQYTQATGLPQLREALSGWYRSHFGLDIPARRIV
                     VTAGASAALQLACLALVNPGDEILMPDPSYPCNRHFVAAAEGIARLLPSGPQQRFQLD
                     AAGVEAAWGSNTRGVLLASPSNPTGTSIDAAEMGRIADCVRRRGGVTMVDEIYLALSY
                     EERYGHSALAHGEDIISINSFSKYFSMTGWRLGWLVLPDELVGPVEKLAQNLYICPSS
                     VAQHAALACFEPDAQAVYQAQRAAFRERRDFIVPALKRLGLDVPVMPDGAFYAWADCS
                     RFSNSSWDFCFELMRSAHLALTPGRDFGRAGVERYLRLSFANALTELEEAVRRLERVL
                     AAR"
     gene            complement(4194..4679)
                     /gene="nusB"
                     /locus_tag="VFV25_05695"
     CDS             complement(4194..4679)
                     /gene="nusB"
                     /locus_tag="VFV25_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEY1392845.1"
                     /translation="MTAEAAKKKTGGAARPKSARRRSREHALQGLYEWLIGGGDAGVI
                     DAHMHEQEGFDKCDRAHFDALLHGCIAEAADLDAVLARHVDRKTTELSPIEHGVLLIG
                     AYELKHCIDIPYKVAINEAVELAKAFGGTDGHKYVNGVLDKAAADLRPTEVDAARGMR
                     R"
     gene            complement(4676..>4834)
                     /locus_tag="VFV25_05700"
     CDS             complement(4676..>4834)
                     /locus_tag="VFV25_05700"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEY1392846.1"
                     /translation="GVTRVSLDGELPIANAILTVENEAQARARAEEKGRDAARVAVEM
                     ANLLEELS"
BASE COUNT          705 a         1575 c         1759 g          795 t
ORIGIN      
        1 gcctcgttga cgcgtctttc gcgcagcttg aagaactcgc gcatgccgga ggcgaagatg
       61 cgctccatgg gcgcggcgcg ctggttgtcg ctggcgacct tggctgcatc gctgtagagc
      121 tcctgcagct ggcggccgga ccagaactcg cgctcgaact cctcgttgtt gccgcgcacg
      181 cggcgcaggc cgaagagctt gccgaagatc accgcccagc tcaccagcga ggtgatcaga
      241 aggccggcca tgacgatctg cacgatgaag ctggcgtgca gcacgaggtt gatgatgttg
      301 aggtcttggc tcatccgatg gcgttcagga tttcgggggg gatgcggctg ggccggaagc
      361 tggcggtatc cacgcagcct atgcgtatcg tgccctcggc cagcagggtg tcgccgcgca
      421 gggcctgttg ttgtaagacg aggctggcgc gcccgcgttc cgtgaggcgc acgctgacgg
      481 tcagcagatc gtcgagccgc gccggctggc ggtagcgcag cgcagtatcg gtcacgacga
      541 acatggtgcc tgtttcagtg cgcaacttct cctgggcgaa gcccagcgag cgcagccatt
      601 cggtgcgcgc ccgctcgaag aacttgaggt agttggcgta gaacaccacg ccgccggcgt
      661 cggtgtcttc ccaatacacg cgcagcgtga agcggtagtc gtcggtgacg gtctcagttt
      721 ctgtcacccc gcgattatct cagtgcgcgg ctactgccga gtcggactca ctgcgccggc
      781 agcggcgtca gtgtgcggcg gtacaccggc ccccagatcg ggtggccagc ctcggacttg
      841 ctcagcgcga agcccgggtc ggcggcgcgc gcggcgcgga aggcggcttc gcattgcacc
      901 gtgcgccggc tggtgcagta gatgaaggcg aggtatttgt tcgcggcagc gcggtccttg
      961 gtcgaggcca ggccggcgcc gagcgcggca ttgaacgact tctcggcctc ggcgtactgg
     1021 ccgtcgtcat aggagcgcat gccccccagc agggctttct cggccgggcg ctggtagaga
     1081 tccatcacgc cgacgggtgc cgccgtcggc ggctggacgc agcagccagc cagtccgaac
     1141 gaaaacaagg ccaggctcgc aagcacgagg cgggagcggc cggcgagcct cacgatccga
     1201 atttgtattt gagcgtggcg ggctgcccgg cgctgaccac gaccgttgcg gtatagggcg
     1261 ggaagtcgcc gttgcgtatc gtgatggtat gccgcccttc ggacagcgtc aggcggttca
     1321 gcggcggcgt cgtgcccgcc ggcgcgccat cgacttcgac atcaccccag gggctgacgg
     1381 cgatgtgcag cacgccggcg gcacccggcg cggccgccgt ggccttggcg gtgcgggcat
     1441 gcgcctcgcg ctgggccttg gctgcccgct cctggcgctg cgcattggcg ggctgggcgg
     1501 ccaatacggt ctcgcccgct ggcacggccg tgggttcagc ctcgatcggc gctgcgccgt
     1561 tcggtgtggg agccgggctc gccgatgcgg ccggcgcggg tgcagccggc gccgctgttg
     1621 tggtggccac ggccactttt gtcgctgccg aggcggcagg tgccgcggcc tgctcggtcg
     1681 catgctcttc gccgccgcgc cagaggccga agccgatcag cgcgaggccg gcggccgcgg
     1741 cggcgccaaa gagcaggcgc ttgcggcgct gcggagcctc gttctcgttg gcctgcagag
     1801 attccggatg ctcgtccagc cagctgcgca gatcgcgtgc cagcgcgcgg gccgagcggt
     1861 agcggtgctc gggttccttc tccatggcgc gcgccgcgat gtcggatagg ccctggggca
     1921 cgcgggtgtc caccgtgtgg gcacgcggcg gctcgcggtt ctgcaccgca ttggaaatct
     1981 cgaccagcga attgccggtg aaagggcgct tgcctgtgag cagctcgtag agcacgacgc
     2041 ccagcgagaa cacgtcggtg cggcgatctg tgggctcctc gcgcatctgc tcgggggcca
     2101 tgtagtaggg cgagccggcc accaggtcga cggcgtcgcg ctgatgcgtg acgcgggcga
     2161 tgccgaagtc gagcaggcgc ggctgcgtgc gcccgaccat gaagatattg gccggcttga
     2221 tgtcgcggtg caccacgccc ttggtatgcg cataggccag cgcatcggcg gcgcggcgca
     2281 cgatgagtgc ggcctgggcc ggggtggggc gccagccttc cttcagcagc tcgcgtaggt
     2341 ccttgccctt gagcaactcc atcgcgatgt aggcgccttg ttcactggtg ccggcgtcga
     2401 acaccgtgac gatgtgcgga tggctcaggc cgccggcggc gcgagcctcg ttcaggaaca
     2461 gggagttgaa ctgctcacgc gcctcggtgc tcgagagctg cacgttcacc atcttgatgg
     2521 cgatcggccg cgacagcagc gggtcgtgag cggcatagac catgcccagg ccgccctcgc
     2581 cgatgcggta tttcagcgca tagcggccga tatggccgat cgtcggcagg gcctcgggag
     2641 gcgcatccgc cgctacgttt aagccgactt cgggcggcag tgtgcgctcg agcagcaccg
     2701 tgtcggcttc gtcccagcct tcggcggcgg gttcggtcga cggtgctggc tccgaatcgc
     2761 cgggcggcag cgatggcaga tcgttgcgag gagagttgga ggggcgttcc acggccgcca
     2821 gcttacgcga agccaggtga cggctggtcc gcaaatccgg gcaatatgtc tcagcttggt
     2881 aacaagcgtg gcgctctcag cgcgcggcaa gcacgcgctc gagccggcgc accgcctcct
     2941 cgagctcggt cagggcgttg gcgaatgaca ggcgcaaata gcgctcgacg ccggcgcggc
     3001 cgaagtcgcg ccccggcgtc agcgcaaggt gggcgctgcg catcagctcg aagcagaaat
     3061 cccagctgct gttcgaaaag cgcgagcaat cggcccaggc atagaaggcg ccatcgggca
     3121 tcacaggcac gtccaggccg agccgcttca gggccggcac gatgaagtcg cgccgctcgc
     3181 ggaaggcggc gcgctgcgct tggtagacgg cctgcgcatc gggctcgaag caggccagcg
     3241 ctgcatgctg ggccacgctg gaagggcaga tgtagaggtt ctgcgcgagc ttctcgaccg
     3301 ggccgaccag ctcgtcgggc agcaccagcc agcccaggcg ccagcccgtc atgctgaagt
     3361 acttcgagaa gctattgatc gaaatgatgt cctcgccatg ggccagcgcg ctgtggccgt
     3421 agcgctcctc gtagctcagg gcgaggtaga tctcgtcgac catcgtgacg ccgccgcggc
     3481 ggcgcacgca gtcggcgatg cgacccatct cggctgcgtc gatcgaggtg ccggtggggt
     3541 tggacggcga ggccagcagc acgccgcgcg tgttcgagcc ccaggccgcc tcgacgccgg
     3601 ccgcgtcgag ctggaagcgc tgctgcggcc ccgagggcag cagccgggcg atgccttccg
     3661 ccgccgcgac gaaatggcgg ttgcagggat agctcgggtc cggcatcagg atttcgtcgc
     3721 cggggttcac caaggcgagg caggccagct gcaaagcggc cgatgcgcct gcggtgacga
     3781 cgatgcgccg cgccgggatg tcgagcccga aatgcgagcg ataccagccg ctcagcgcct
     3841 cgcgcagctg gggcaggccg gtggcctggg tgtattgcgt gcggccagcg cgcaggcagc
     3901 gctgcgcggc ctcgaccacg ctgggcgggg ccgtgaaatc gggctcgccg atgttgagga
     3961 agatcatcgg ctcgccgccc tggcgcgggt cgcaagccgg gctggcggcg atctcggccg
     4021 ccgccttggc gcattccatc acgtagaagg gctcaatgtg agccaggcgc gccgccagat
     4081 tcatgacgac ccctcgaccg ccgagtacgt cggccactcc cccgcggtga gcgggccttg
     4141 cttggggcgg cccggcgcgg cggcccggag ggctcgtgtt gacactgggt gccttagcgg
     4201 cgcataccgc gcgctgcgtc gacttccgtg gggcgtaggt ctgcggccgc cttgtcgagc
     4261 acgccgttga cgtatttgtg gccgtcggtg ccgccgaagg ccttggcgag ctccacggcc
     4321 tcgttgatcg cgaccttgta ggggatgtcg atgcagtgct tgagctcgta ggcgccgatc
     4381 agcagcacgc catgctcgat gggcgaaagc tcggtggtct tgcggtcgac atggcgggcc
     4441 agcacggcat cgaggtccgc ggcctcggcg atgcaaccgt gcagcagtgc atcgaaatgc
     4501 gcgcggtcgc acttgtcgaa gccttcctgc tcatgcatgt gcgcatcgat cacgccggcg
     4561 tcgccgccgc cgatcagcca ttcgtacagg ccctgcagcg cgtgctcgcg cgaacggcgg
     4621 cgcgccgact tgggacgggc tgcgccgccc gtcttcttct ttgcagcttc tgcggtcacg
     4681 acaactcttc cagcagattg gccatctcga ccgcgacgcg cgccgcgtca cgacccttct
     4741 cctcggcgcg ggcccgggcc tgggcctcgt tctcgaccgt gaggatggca ttggcgatcg
     4801 gcagctcgcc gtccagcgag acgcgtgtca cgcc
//
LOCUS       DASUCY010000294         3455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_6365, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000294 DASUCY010000000
VERSION     DASUCY010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6365"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1263
                     /locus_tag="VFV25_05705"
     CDS             <1..1263
                     /locus_tag="VFV25_05705"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HEY1392847.1"
                     /translation="VVGNEEHRFLVLEQLRELGTGPAALLLEPLGRNTAPALTLAALQ
                     AVEDGSDPILVVSPADQTVTDGAAFTAALQQAMRVAAGGTIVILGIPPDRPETGYGYI
                     RSGAADGASAPVLQFVEKPDLATAQRYLAEGDYSWNSGLFVLKASTWLAALQAFRPDI
                     AAATRAAWAARRSDAKFVRPGKTEFAAVPADSIDYAVMERCPGSAFELRMVSLAAGWS
                     DLGAWDAVWQVADKDEAGNARRGDVLLHEARNSLVQATSRLVSVVGLDDVVVVETPDA
                     VLVSRRGRSQEVKAIVEQLQAQKRGEQALHRKVHRPWGWYDSIDAGPRFQVKRIQVNP
                     GASLSLQKHHHRAEHWIVVKGTAEVSCGDKQLLLTENQSTYIPLGEVHRLANPGKLAL
                     EIIEVQSGAYLGEDDIVRFEDNYGRSAA"
     gene            1574..2209
                     /locus_tag="VFV25_05710"
     CDS             1574..2209
                     /locus_tag="VFV25_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system exopolysaccharide export
                     protein"
                     /protein_id="HEY1392848.1"
                     /translation="MFSTAAVRCARKLLACLGLCAVALMLAACASNQYPPAPVSADNP
                     EYRYLIGPLDSLNIVVWRNPELSGVFTVRPDGRISTPLVEDLPAVGRSPQDLARDIEK
                     ALSKYTRDPVVSVVVTGFQGSASEQIRVIGEAAKPQAIPYRQNMTMLDVMIQVGGLTD
                     FADGNHAVLVRGKENGKQYSLRLKDLIKRGDISANVEVRPGDVVIIPQSWF"
     gene            2246..>3455
                     /locus_tag="VFV25_05715"
     CDS             2246..>3455
                     /locus_tag="VFV25_05715"
                     /inference="COORDINATES: protein motif:HMM:TIGR03007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA system polysaccharide chain length
                     determinant"
                     /protein_id="HEY1392849.1"
                     /translation="MDELIEQALSVVRGMWRRRWAGLLAAWVVALIGALVLLRTPDRY
                     EANARVYVDSKSVLKPLMRDLAVEPDLEQTLSLLTHTLITRPNIELLIRKASLDAPSA
                     SQDDHELLVEGLTRDIKLAGSGRDNVFSFSYRSTSPAIAQRVVESLVALFVESDLGAK
                     ARDAESARGFIDQQIKAYEARLTEAENRLKEFKLRNLEITDTGGKDYFARIAALNEEL
                     SKQSVELRAAEQSRDALKRQLSGENESLLPDLSAQAAVPLTPEIDARLDTQRRQLDEL
                     LRRYTDLHPDVIATKRLIAQLEEQKAKEIEAHKRAAANRPPSPATVSPVMQQVQLALA
                     EAEGNVAALRVRQGETQARLTQLRAAANRVPQVEAELAQLNRDYDVVRSNYQALVARR
                     EKASMSEEVDT"
BASE COUNT          606 a         1168 c         1130 g          551 t
ORIGIN      
        1 gtggtgggca acgaggagca ccgctttctc gtgctcgagc agctgcgcga actcggcact
       61 gggcccgccg ccctgctgct cgaacccctg ggccgcaaca ccgccccggc cctcaccctg
      121 gccgctctgc aggcggtcga agacggcagc gaccccatcc tcgtggtcag ccccgccgac
      181 cagaccgtca ccgatggcgc cgccttcacc gctgcactgc agcaagccat gcgcgtggcc
      241 gctggcggca ccatcgtcat cctgggcatc ccccccgacc gccccgagac cggctacggc
      301 tacatccgca gcggcgccgc cgatggggcc tcagccccgg tcctgcagtt cgtcgagaag
      361 cccgacctgg ccaccgccca gcgctacctc gccgaaggcg actacagctg gaactccggc
      421 ctgttcgtgc tcaaggcttc cacttggctg gccgcgctgc aggccttccg ccccgacatc
      481 gccgccgcca cccgcgccgc ctgggccgcg cgccgcagcg atgccaagtt cgtgcgcccg
      541 ggtaaaaccg agttcgccgc cgtgccggcc gactcgatcg actacgccgt gatggagcgc
      601 tgcccgggca gcgccttcga gctgcgcatg gtgagcctgg ccgccggctg gagcgacctg
      661 ggggcctggg acgccgtgtg gcaggtggcc gacaaggatg aggccggcaa cgcccgccgc
      721 ggcgacgtgc tgctgcacga ggcccgcaac agcctggtgc aggccaccag ccggctggtc
      781 agcgtggtgg ggctggacga cgtggtggtg gtggagaccc ccgatgcggt gctggtcagc
      841 cggcgcgggc gcagccagga ggtcaaggcc atcgtcgagc agctgcaggc gcagaaacgc
      901 ggcgagcagg ccctgcaccg caaggtgcac cggccctggg gctggtacga cagcatcgat
      961 gcggggccgc gcttccaggt caagcgcatc caggtgaacc cgggggcctc cttgagtctg
     1021 cagaagcacc accaccgcgc cgagcattgg atcgtcgtga agggcacggc cgaggtgagc
     1081 tgcggggaca agcagctctt gctcaccgag aaccagagca cctacatccc gctgggcgag
     1141 gtgcatcgcc tcgccaaccc gggcaagctg gcgctggaaa tcatcgaggt gcagtccggc
     1201 gcctacctcg gcgaggacga catcgtgcgc ttcgaggaca actacgggcg cagtgctgcc
     1261 tgacacgcaa gccgcggcgc tgccgttcac gcagcggtct cacattcatg acaacggtgt
     1321 gatgcgcctc gcctcaggca gcgatttcgt cagctaccca caacgccgat gagggggata
     1381 gacccccccg tttagggtcg tttcattcac gctgtcgcca cgtattcttc atgcgctcct
     1441 cggactgagc gcttctcgac acatagcggg cagccggcgg cgtcagcggc cctgcgccgg
     1501 agctatcacc tcgtccgaaa tcggcacgcg aacgcgctgc ccggcaggac aggagcaagg
     1561 actggaggat tccatgtttt cgacagccgc tgttcgttgt gccaggaagc tgctcgcctg
     1621 cttgggcttg tgcgccgtcg cgctcatgct tgcggcctgc gcttccaacc agtatccgcc
     1681 ggctcccgtg tcggccgata accccgaata ccgctacctg atcggccctc tggattcgct
     1741 caacatcgtc gtgtggcgca acccggagct gtctggtgtc ttcacggttc gccccgacgg
     1801 gcgcatttca acgccgctgg tcgaggatct gccggcggtg ggccgcagcc cgcaggactt
     1861 ggcgcgcgat atcgaaaagg ccttgagcaa gtacacgcgc gaccccgtgg tcagcgtcgt
     1921 cgtcacgggt ttccagggct ctgccagcga gcagatccgc gttatcggcg aggctgcaaa
     1981 gccacaggcg attccttatc gccagaacat gaccatgctc gacgtgatga tccaggtcgg
     2041 cgggctcacc gatttcgccg atggcaacca cgcggtgctg gtgcgcggca aggaaaacgg
     2101 caagcagtac agcctgcgcc tgaaggatct gatcaagcgc ggcgatatct cggccaatgt
     2161 ggaggtacgc ccgggcgatg tcgtgatcat tccccagagc tggttctgac aacagcagcg
     2221 atcaaccgag tccttccttt tcaccatgga tgaactgatc gagcaggcgc tgagtgtggt
     2281 tcgcggcatg tggcgccgtc ggtgggcggg gctgctggca gcctgggtcg ttgcgctgat
     2341 cggtgcgctg gtcttgctgc gcacacccga tcgctacgaa gccaatgcgc gcgtttatgt
     2401 cgactccaag agtgtgctca agccgctgat gcgcgacctg gcggtcgagc cggatctcga
     2461 gcagaccctc agtctgttga cccacacgct gatcacgcgg cccaatatcg agttgctgat
     2521 acgcaaggcg agcctggacg cgccaagcgc gtctcaggac gaccacgagc ttctggtcga
     2581 aggcttgacg cgcgacatca agctggctgg cagcggtcgc gacaacgtct tcagcttcag
     2641 ctatcgcagc accagcccgg cgatcgccca gcgcgtggtc gagagcctgg tggccttgtt
     2701 cgtcgaatcc gacctgggtg ccaaggcccg tgacgccgaa tcggcgcgcg gcttcatcga
     2761 ccagcagatc aaggcctatg aggctcgtct gaccgaagcc gagaaccgcc tgaaggaatt
     2821 caagctgcgc aatctggaga tcaccgacac cggcggcaag gattacttcg cgcgcattgc
     2881 ggctctcaac gaggagttga gcaagcaaag cgtcgagttg cgcgcggcgg agcagtcgcg
     2941 cgacgcgctg aagcgtcagc tttccggcga aaacgaatcg ctgctacctg acttgtctgc
     3001 gcaggccgcc gtcccgctga ccccggagat cgatgcacgc cttgacaccc agcggcgcca
     3061 gctcgatgaa ctgctgcggc gctacaccga cctgcacccc gacgtcatcg ccaccaagcg
     3121 cctgatcgcc cagctggagg aacagaaggc caaggaaatc gaggcgcaca agcgtgccgc
     3181 ggcgaaccgg ccgccatcgc cggccacggt gagcccggtg atgcagcagg ttcagttggc
     3241 attggccgag gcggaaggca atgtggcggc cctgcgcgtg cgacagggtg aaacccaggc
     3301 gcgtctgacc caactgcgtg ccgccgccaa tcgcgtgccc caggtggagg cagagctggc
     3361 tcagctcaat cgcgactatg acgtcgtgcg cagcaactac caggccctgg tggcgcggcg
     3421 cgagaaggcc tcgatgtccg aggaggtcga tacga
//
LOCUS       DASUCY010000295         2512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_841654, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000295 DASUCY010000000
VERSION     DASUCY010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841654"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="VFV25_05720"
     CDS             <1..828
                     /locus_tag="VFV25_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY1392850.1"
                     /translation="DAFCKQMLQYYPAPFINLMRYLSVICIAFAMLLRHGDLRIWRAP
                     RKKLLVLRGVMLGTVATCFMTALIWMPLAEATAIYFTAPLLMVALSPWLVGEQVRRSR
                     WVAVTVGFGGMLLIVRPGGDLPWLGTVLMAVSAGCYAIFQLLTRRLAGLVPSPVQYGY
                     TALVCLVVTALPAPFFLPVHRPAPGEVLLLLAGGACSGAAQWLLLAAFERVEASTLAP
                     LNYFQLLLAVAFSTFWFHKPPDGLAMSGIALIVAAGIYLARARPAGPAVLAAARTGR"
     gene            873..1589
                     /locus_tag="VFV25_05725"
     CDS             873..1589
                     /locus_tag="VFV25_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY1392851.1"
                     /translation="MTFSSPFDTASFESVEAPDLVELVEAQLTRAIVEGRLAPGSRIV
                     EADIARRMGVSRAPVREAARRLERQGVLVARPRHGFAVRTISVQEIDDIFEVRISLEM
                     TAIERACSRADDAGLAHLQELVHAMVREAPTQPQHQRIATDLAFHTLICELSGNAHLH
                     RIFSNTQTEMRMIIALCDAVYHDPSAMAETHQPIADALMRRDAAAACAAMRLHLDDAW
                     QHVRALFVKQHGAASAPSNP"
     gene            1607..>2512
                     /locus_tag="VFV25_05730"
     CDS             1607..>2512
                     /locus_tag="VFV25_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEY1392852.1"
                     /translation="MKVVYSDQHKDHDPQLFMVRGRLKRSNEQPERAFRLLSAVQDGG
                     HDIIACEDFGAGPRAAIHTPEYLRFLETAYERWQGLDDPSQEVMPNIHPFPGEPCTYP
                     ESVVGQAGFHMGDNACSIGPHTWQAAIASANVATHAAQLVADGERAAYALCRPPGHHA
                     YADRANGFCYLNNVAIAAQHLRRVHDRVAILDIDVHHGNGTQGIFYRRPDVLTISLHG
                     DPRNFTPFFTGHAHEKGEGAGLGYNINKPLALGTDMAGYLPVLRDACLSIRAFAPGAL
                     VVALGLDAHERDPYKGMKITTQDFAL"
BASE COUNT          400 a          890 c          795 g          427 t
ORIGIN      
        1 gacgccttct gcaagcagat gctgcagtac tacccggcgc ccttcatcaa cctgatgcgc
       61 tacctgtcgg tgatctgcat cgccttcgcc atgctgctgc gccacggcga cctgcgcatc
      121 tggcgggcac cgcggaaaaa actgctggtg ctgcgcggcg tgatgctggg cacggtggcc
      181 acctgtttca tgacggcatt gatctggatg ccgctggccg aggccacggc gatctacttc
      241 accgcgccgc tgctcatggt ggcgctgtcg ccctggctgg tcggcgagca ggtgcggcgc
      301 agccggtggg tagctgtgac ggtcggtttt ggcggcatgc tgctgatcgt gcgtcccggc
      361 ggcgacctgc cctggctggg cacggtgctg atggctgttt cagccggctg ctacgcgatc
      421 tttcagcttc tcacgcggcg cctcgcgggg ctggtgccca gccctgtgca gtacggctat
      481 acggcgctgg tctgcctggt ggtgaccgcc ctgccggcgc ccttcttcct gccggtccac
      541 cggcctgcgc cgggcgaggt cctgctgctc ctggccggcg gcgcctgcag cggtgccgcg
      601 cagtggctgc tgctggccgc cttcgagcgg gtggaggcgt ccaccctcgc gcccctgaac
      661 tacttccagc tgctgctcgc cgtcgccttc agcactttct ggttccacaa gccgcccgat
      721 ggtctcgcga tgtccggcat tgcgctgatc gtggccgcag gcatctacct ggcgcgggcc
      781 cggccggcgg gccccgccgt cctggccgcc gcgcgcacag gcaggtaaga aaccatgttc
      841 cggcgcgcag accccataac gagacaaaca gcatgacttt ttcatcccca ttcgataccg
      901 cttcctttga atccgtcgag gcccccgatc tggtcgagct ggtcgaggcc caattgacgc
      961 gcgccatcgt ggaggggcgc ctggcgcccg gcagccgcat cgtggaggcc gacatcgcgc
     1021 gccgcatggg cgtgagccgg gcgccggtgc gcgaggccgc gcgccggctg gagcgccaag
     1081 gcgtgctggt cgcgcggcca cgccacggct ttgcggtgcg gaccatcagc gtgcaggaga
     1141 tcgacgacat cttcgaggtg cgcatcagtc tcgaaatgac ggcgatcgag cgggcctgca
     1201 gcagggccga cgacgctggg ctggcccatc ttcaggaact cgtgcacgcc atggtgcgcg
     1261 aggcgcctac gcagccgcag caccagcgaa tagccaccga tctggcgttc cacaccctga
     1321 tctgcgaact gtcgggcaac gcccacctgc accgcatctt ctccaacacc cagaccgaga
     1381 tgcggatgat cattgcattg tgcgatgccg tctaccacga cccatccgcc atggccgaga
     1441 cccatcaacc catcgccgat gcgctgatgc gcagagacgc agcagccgcc tgtgctgcca
     1501 tgcgcctgca tctggacgac gcctggcagc atgtgcgggc gctgttcgtg aagcagcacg
     1561 gcgccgcgtc cgcaccttcc aacccctgat tccgaggacc ccttcgatga aagtcgtcta
     1621 cagcgatcag cacaaagacc acgacccgca actcttcatg gtgcgcggtc gattgaagcg
     1681 cagcaatgaa cagccggaac gcgcctttcg cctgctgtcg gcggtccagg acggcggcca
     1741 tgacatcatc gcctgcgaag acttcggcgc gggcccgcgt gccgccatcc acacgccgga
     1801 gtacctgcgc tttctcgaaa ccgcttacga acgatggcag gggctggacg acccctcgca
     1861 agaggtcatg cccaacatcc atccctttcc cggcgagccc tgcacctacc ccgagagcgt
     1921 ggtgggccag gccggattcc acatgggaga caacgcctgc tccatcggcc cgcatacctg
     1981 gcaggcggcc atcgcctcgg ccaatgtggc cacgcatgcg gcgcagctgg tggcggacgg
     2041 cgagcgcgcc gcctacgcgc tgtgccgccc gccgggtcac catgcctacg cggaccgggc
     2101 gaatggcttt tgctacctga acaacgtggc catcgccgcg cagcacttgc gccgggtgca
     2161 cgaccgcgtc gccatcctcg acatcgacgt gcaccacggc aacggcacgc agggcatctt
     2221 ctaccgccgc ccggacgtgc tgaccatttc actgcatggt gacccccgca acttcacgcc
     2281 cttcttcacc ggtcatgcgc atgagaaggg agaaggcgca ggcctgggct acaacatcaa
     2341 caaacccctg gcgctgggaa ccgacatggc cggctacctg ccggtattgc gcgatgcctg
     2401 cctgagcatt cgcgccttcg cacccggtgc gctggtggtg gcgctgggcc tggacgccca
     2461 cgaacgcgac ccgtacaagg gcatgaagat cacgacacaa gacttcgcgc tg
//
LOCUS       DASUCY010000296         3145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1452065, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000296 DASUCY010000000
VERSION     DASUCY010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1452065"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1091
                     /locus_tag="VFV25_05735"
     CDS             <1..1091
                     /locus_tag="VFV25_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY1392853.1"
                     /translation="DEVRKHTKASASCGSCTGLVEQLIMFTAGGDFSAAPKKKAMCGC
                     TDHSHGDVRKAIREHKLLTIDSVYKFMEWRTPNGCSSCRPAVNYYLISTWPKEAKDDP
                     QSRFINERSHANIQKDGTYSVIPRMWGGETTSSELRRIADVVDKYQIPTVKVTGGQRI
                     DLLGVKKEDLQNVWRDIGMPSGHAYAKALRTVKTCVGSEWCRFGTQDSTQMGKDLERA
                     LWRMYAPHKVKLAVSGCPRNCAESGIKDVGVIGVDSGWEIHVGGNGGIKTEVAQFLVK
                     VKTREEVLEYAGAFLQLYREEGWYLERTCHYIARVGMDHVKKHILDDAEGRKALWERL
                     QFALDGEPDPWFEFDKAAVDTRQFAPVA"
     gene            1105..1893
                     /locus_tag="VFV25_05740"
     CDS             1105..1893
                     /locus_tag="VFV25_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HEY1392854.1"
                     /translation="MQRRELLALAALALAGGARAQVQDIGDAINKAGRQRMLSQRCAK
                     AYLALGMELTDAGARQVLDASLALFDRQLVELKVFAPNAAIKSTYQELESVWSDYKAA
                     LVGAKPAHERVPALIELDGRVLLLANKGTGLYEAVSGRSQGRLVNIAGRQRMLSQRCA
                     KFYLALAWNAGVGNAKAELDKARGEFVAAHRVLADAPEATAEIRAELELAQNQWVFFD
                     NALQQGNASGQAGRRASEVFRASEYVLQVMDRVTGMYARLTAGA"
     gene            1912..2298
                     /gene="nirD"
                     /locus_tag="VFV25_05745"
     CDS             1912..2298
                     /gene="nirD"
                     /locus_tag="VFV25_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HEY1392855.1"
                     /translation="MSDWKPICRVDDIPVLGARRVERSNACAVAVFRNAEDKVFALLD
                     RCPHKGGPLSQGIVFGESVACPLHNQVIGLDDGCAKAPDEGCTPRFAVKLEAGTVYLD
                     TGELASLGTDLPKPLAGPALKAAASI"
     gene            2420..>3145
                     /locus_tag="VFV25_05750"
     CDS             2420..>3145
                     /locus_tag="VFV25_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY1392856.1"
                     /translation="MAETRSTCPYCGVGCGVIIESEGEQITGVRGDPEHPANFGRLCS
                     KGSTLHLTATAPITLQTRLLQPQRRAARGEAPQPVSWEAALDEAADRFAGIIKKHGPD
                     AVGFYISGQLLTEDYYVFNKLAKGLIGTNNVDTNSRLCMSSAVAGYKATLGADAPPAC
                     YDDLKHAQTVFIVGSNAAYAHPVLFRRLEDAKRANPSLKLIVADPRRTETAEAADLYL
                     PILPGTDVALFHGMLHWLMWEGLT"
BASE COUNT          564 a         1066 c         1049 g          466 t
ORIGIN      
        1 tggatgaggt gcgcaagcac accaaggcca gcgcctcctg cggttcctgc accggcctgg
       61 tcgagcagct catcatgttc accgccggcg gcgatttttc cgccgcgccc aagaagaagg
      121 cgatgtgcgg ctgcaccgac cacagccacg gcgacgtgcg caaggcgata cgcgagcaca
      181 agctgctgac gatagacagc gtctacaagt tcatggaatg gcgcacgccc aacggctgct
      241 cgtcctgccg gccggccgtc aactactacc tcatcagcac ctggcccaag gaggccaagg
      301 acgacccgca atcgcgcttc atcaacgagc gcagccacgc caacatccag aaggacggca
      361 cttattcggt cattccgcgc atgtggggcg gcgagaccac ctcgtcagaa ctgcgccgca
      421 tcgccgacgt ggtggacaag taccagatcc ccaccgtgaa ggtcactggc ggccagcgca
      481 tcgacctgct gggcgtgaag aaggaagacc tgcagaacgt ctggcgcgac atcggcatgc
      541 cctcgggcca tgcctatgcc aaggcgctgc gcaccgtgaa gacctgcgtc ggcagcgagt
      601 ggtgccgctt cggcacccag gacagcacgc agatgggcaa ggacctggag cgcgcgctgt
      661 ggcgcatgta tgcgccgcac aaggtcaagc tcgcggtgag cggctgcccg cgcaactgcg
      721 ccgagtcggg catcaaggac gtgggcgtga tcggcgtcga ctcgggctgg gagatccacg
      781 tcggtggcaa cggcggcatc aagaccgagg tcgcgcagtt cctcgtgaag gtgaagacgc
      841 gcgaggaggt gctggagtac gccggtgcct tcctgcagct ctatcgcgag gagggctggt
      901 atctggagcg cacctgccac tacatcgcac gcgtcggcat ggaccacgtg aagaaacaca
      961 tcctcgacga cgccgaaggc cgcaaggcgc tgtgggagcg gctgcagttc gcgctcgacg
     1021 gcgagcccga tccctggttc gagttcgaca aggcggcggt cgatacgcgc cagttcgctc
     1081 cagtggcctg aaggagtact cgcgatgcaa cgacgtgagc tcctcgccct ggctgcgctt
     1141 gcgctggccg gcggtgcgcg tgcccaggtg caggacatcg gcgacgcgat caacaaggcg
     1201 ggccggcagc gcatgttgtc gcagcgctgc gccaaggcct acctggcgct cggcatggaa
     1261 ctgaccgacg cgggggcacg ccaagtgctc gacgcttcgc tcgccctgtt cgatcgccag
     1321 ctggtcgagc tgaaggtctt cgcacccaac gccgccatca agtcaaccta tcaagagctc
     1381 gaaagcgtct ggagcgatta caaggcggcg ctggtcggcg ccaagccggc gcacgagcgc
     1441 gtgccggcgc tgatcgagct cgatggccgc gtgcttttgc tggcgaataa aggcaccggc
     1501 ctgtacgaag cggtatctgg cagatcgcaa ggccggctgg tcaacatcgc cggccgtcag
     1561 cgcatgctgt cgcagcgctg cgcgaagttc tacctcgcac tggcgtggaa tgccggcgtg
     1621 ggtaacgcca aggccgagct cgacaaggcg cgcggtgaat tcgtcgccgc gcatcgcgtg
     1681 ctggccgacg cacctgaagc caccgccgag atccgcgccg agctggaact ggcgcagaac
     1741 cagtgggtgt tcttcgacaa cgccctgcag cagggcaacg cctccggcca ggccggccgc
     1801 cgcgcgtcag aggtgtttcg cgccagcgaa tacgtgctgc aggtgatgga ccgggtgacc
     1861 ggcatgtatg cgcggctgac ggccggcgct tgaagcagga gcgaaatcat catgagtgac
     1921 tggaaaccca tctgccgcgt cgacgacatc ccggtactcg gcgcgcgccg cgtggagcgc
     1981 agcaacgcct gtgcggtggc ggtattccgc aacgccgagg acaaggtctt cgccctgctc
     2041 gaccgctgcc cgcacaaggg cggtccgctg agtcagggca tcgtgttcgg cgagagcgtg
     2101 gcctgcccgc tgcacaacca ggtgatcggc ctggacgacg gctgcgccaa ggcgcccgac
     2161 gaaggctgca cgccgcgctt cgcggtcaag ctcgaggcgg gcaccgtcta cctcgatacc
     2221 ggcgagctgg ccagcctcgg caccgatctg ccgaagccgc tggccggacc ggcgctgaag
     2281 gcagctgcca gcatctgatc tgcgcaaagt catgcgcgaa acccgttcga cttgccccta
     2341 ctgcggacac gcctgcggcg gtccgctttg cggtgcacgg gggctccccc accgccccct
     2401 ccgagagggg gcctaagtca tggccgagac ccgttcgact tgcccctact gcggcgtcgg
     2461 ctgcggcgtg atcatcgaga gcgagggcga gcagatcacc ggcgtgcgag gcgaccccga
     2521 gcacccggcc aacttcggcc ggctgtgcag caagggcagc acgctgcacc tcaccgcaac
     2581 cgcgcccatc acgctgcaga cgcggttgct gcagccgcag cgccgcgccg cgcgcggcga
     2641 agcgccgcag ccggtgagtt gggaggcagc gctcgacgag gccgcggacc gcttcgccgg
     2701 catcatcaag aagcacggcc ccgatgccgt gggcttctac atctcgggcc agctgctgac
     2761 cgaggactac tacgtcttca acaagctggc caagggcctg atcggcacca acaatgtcga
     2821 caccaactcg cggctgtgca tgtcgagcgc ggtcgccggc tacaaggcca cgctgggcgc
     2881 cgatgcgccg ccggcctgct acgacgacct gaagcatgcg cagacggtct tcatcgtcgg
     2941 ctcgaacgcc gcctacgcgc acccggttct gttccgccgg ctggaggatg ccaagcgcgc
     3001 caatccgtct ctgaagctca tcgtcgccga cccgcgccgc accgagaccg ccgaggcggc
     3061 cgacctgtac ctgcctatcc tccccggcac cgatgtggcg cttttccacg gcatgctgca
     3121 ctggctgatg tgggagggcc tgacc
//
LOCUS       DASUCY010000297         2899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_231085, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000297 DASUCY010000000
VERSION     DASUCY010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231085"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="VFV25_05755"
     CDS             complement(<1..406)
                     /locus_tag="VFV25_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEY1392857.1"
                     /translation="MTAAASLLLDGPAFVRGWLMSAGLIVAIGAQNALVLRQGLARSH
                     VGLVVALCVASDWLLIAAGVYGLGALIAASPQALELLRWGGAAWLIGYGALAAKRALA
                     SEHAVLSGATGAGSLKATLATTLAMTWLNPHVY"
     gene            480..1469
                     /locus_tag="VFV25_05760"
     CDS             480..1469
                     /locus_tag="VFV25_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HEY1392858.1"
                     /translation="MKDLDPAALDCLAALADEGGFERAAQRLAITQSAVSQRLRAMEA
                     QIGQPLVVRSRPLRLTESGKVLLRLARQWHALRADAARELGGSGGGADERLPIAVNAD
                     SLATWVLPALDALVQEGQRAGYGLELVVDDQDFTHDWLRQGAVLGCVSSVSQALRGCR
                     VTPLGVMRYCAVASPAFVDKLLGGDARRGLHEGNFASAPFLVFNRKDDMQAQWVRRAF
                     GVRAPRLNERFVPSSEAVMQAAVMGWGVAALPELQARDAIARGELLVLKPEVAVDVAL
                     YWHQWKLGPEETQGQRSALLDRIGEALVQGAGKALGPQRKQAGTTSVAARRRA"
     gene            complement(1443..1661)
                     /locus_tag="VFV25_05765"
     CDS             complement(1443..1661)
                     /locus_tag="VFV25_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392859.1"
                     /translation="MTQLSLQMLTWGTVAGAVASVVMLVAPGDWPVSRAAHTGIMMVR
                     PAPVLGHRAVGDLRRRHAAPSGAAPRSN"
     gene            complement(1850..2626)
                     /locus_tag="VFV25_05770"
     CDS             complement(1850..2626)
                     /locus_tag="VFV25_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY1392860.1"
                     /translation="MRLQIYSDLHLETEAFEPEPASGAELLVLGGDIDSNWTGLERFK
                     GWPVPVIYVAGNHEFDGRELNDAWPALRERCEALGFTMLERESIVLRGEDGRRLRFVA
                     TVRWADFDLFGVANRERSMRASRYFMEIMGATRGGDFFDSAAMREEALVCREWLAAEL
                     ARPSEGQWDATVAITHFGPSLRSADPRYGKQPGTASFCNADDDLLPLAELWIHGHLHC
                     RHDYRVTHADGSATRVVSNARGHSRRREAEGYNALFCVDV"
     gene            complement(2636..>2899)
                     /locus_tag="VFV25_05775"
     CDS             complement(2636..>2899)
                     /locus_tag="VFV25_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEY1392861.1"
                     /translation="LDSIWRHLPEQDETRRAMTQALDKEHPLPNLDAAIEALVFDIVD
                     LHQIATAERLKVATVKREMPKVGRNDPCPCGSGRKYKLCHGMT"
BASE COUNT          448 a          986 c         1025 g          440 t
ORIGIN      
        1 gatagacatg cggattcagc caagtcatcg ccaaggtcgt ggcgagcgtg gccttgaggc
       61 tgcccgcccc agtggcgcca ctcagcacgg catgctcgct ggcgagcgca cgcttcgccg
      121 ccaaggcgcc atagccgatc agccaggccg caccgcccca gcgcagcagc tccagggcct
      181 gcggcgaggc ggcgatcagc gcacccaggc catacacgcc ggcggcgatc agcagccagt
      241 cggaggccac gcacagcgcc acgaccaggc ccacatggct gcgcgccagc ccttggcgca
      301 gcaccagcgc attctgcgcg ccgatcgcga cgatcaggcc ggccgacatc agccagcccc
      361 ggacaaaggc ggggccgtcg agcagcaggg aggcggcggc agtcattccc ggattctcgg
      421 cagaggccat gcaaacatga agccgcatgc atattcttca gattacatta gcagtgctta
      481 tgaaagacct ggatcccgct gcgctcgatt gcctggccgc gctggccgac gaaggcggct
      541 tcgagcgcgc ggcgcagcgg ctggcgatca cgcaatcggc cgtgtcgcag cggctgcgcg
      601 cgatggaagc gcagatcggc cagccgctgg tggtgcgctc gcgtcccttg cgcctgaccg
      661 aatcgggcaa ggtgctgctg cgcctggccc gccaatggca cgcgctgcgc gccgacgcgg
      721 cgcgcgagct cggcggcagc ggcggtggtg ccgacgagcg cctgccgatc gcggtgaatg
      781 ccgacagcct cgccacctgg gtgctgccgg cgctggatgc actggtgcag gaggggcagc
      841 gcgcgggcta cggcctggag ctggtggtgg acgaccagga cttcactcac gactggctgc
      901 gccagggcgc cgtgctgggc tgcgtctcca gcgtcagcca ggccttgcgc ggctgccgcg
      961 tgacgccgct gggcgtgatg cgctattgcg ccgtcgcgag ccccgccttc gtcgacaagc
     1021 tgctgggcgg cgacgccagg cgcggcctgc atgaaggcaa tttcgcgagc gccccttttc
     1081 tcgtgttcaa ccgcaaggac gatatgcagg cgcaatgggt caggcgcgcc ttcggcgtgc
     1141 gcgccccgcg gctgaacgag cgcttcgtgc cttcatccga ggcggtcatg caggccgccg
     1201 tgatgggctg gggcgtggcc gcactgcccg agctgcaggc gcgcgacgcc atcgcgcgcg
     1261 gcgagctgct ggtgctcaag cccgaagtcg cggtggacgt ggccttgtac tggcaccagt
     1321 ggaagctggg gcccgaggag acgcagggcc agcgcagcgc gctgctcgac cgcatcggcg
     1381 aagccctggt gcaaggggcc gggaaggccc tggggccgca gaggaagcag gctggtacta
     1441 cttcagttgc tgcgcggcgc cgcgcctgaa ggcgcggcat gccgccgccg cagatcgccc
     1501 acagccctgt ggcccaggac cggtgcgggg cgcaccatca tgatgccggt atgcgccgca
     1561 cgcgacaccg gccagtcacc cggtgcgacc agcatcacga cgctcgcgac ggcgccggcg
     1621 acggtacccc aggtcaacat ctgtagcgac agttgagtca tggcggtggc tggtggactg
     1681 cccggcgtgg atgcgaaaac ggcttcgtgc gatgaagctt agtctgcaca tctttgcatt
     1741 cccatccatc cgaaggggga gtttctgcag cctcgtcagc gacttgcaag tcgcgcgcca
     1801 ggcagccccc aagttctagg gattgcgcgc tggcgccgtt gcgccgcccc tagacatcca
     1861 cgcaaaaaag cgcgttgtag ccttcggctt cgcggcgccg gctgtggccg cgcgcattgc
     1921 tgaccacgcg ggtcgcgctg ccgtcggcat gcgtcacgcg gtagtcgtgg cggcagtgca
     1981 ggtggccgtg gatccacagc tcggccagcg gcagcagatc gtcgtcggca ttgcagaagc
     2041 ttgcggtgcc gggctgcttg ccgtagcgcg gatcggcgct gcgcaggctg gggccgaaat
     2101 gcgtgatcgc gaccgtcgcg tcccactgcc cctcgctggg ccgcgccagc tcggcggcga
     2161 gccattcgcg gcagaccaac gcctcctcgc gcatcgcggc gctgtcgaag aaatcgccgc
     2221 cgcgggtggc ccccatgatc tccatgaagt agcgcgaagc gcgcatgctg cgctcacggt
     2281 tcgcgacgcc gaagagatcg aagtcggccc agcgcacggt ggcgacgaag cgcagccgcc
     2341 ggccgtcctc cccccgcagc acgatgcttt cgcgctccag catcgtgaag cccagcgctt
     2401 cgcagcgctc gcgcagcgcg ggccaggcgt cgttcagctc gcgcccgtcg aactcgtggt
     2461 tgcccgcgac atagatcacc ggcaccggcc agcccttgaa gcgctccagg ccggtccagt
     2521 tgctgtcgat gtcgccgccc agcaccagca gctcggcgcc ggaagcgggt tcgggctcga
     2581 aggcctcggt ttcgagatgc aggtcggaat agatctgtaa gcgcatcgag ctgggctagg
     2641 tcatgccgtg gcagagcttg tacttgcgcc cgctgccgca ggggcaggga tcgttgcggc
     2701 cgaccttggg catctcgcgc ttgacggtgg cgaccttcag gcgctcggcc gtggcgatct
     2761 ggtgcaagtc cacgatgtcg aacaccaggg cttcgatggc ggcgtcgagg ttcggcagcg
     2821 ggtgctcctt gtccagagcc tgggtcatcg cacggcgcgt ctcgtcctgc tcgggcaggt
     2881 ggcgccaaat cgaatcgag
//
LOCUS       DASUCY010000298         3078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_648938, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000298 DASUCY010000000
VERSION     DASUCY010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648938"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1181)
                     /locus_tag="VFV25_05780"
     CDS             complement(<1..1181)
                     /locus_tag="VFV25_05780"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY1392862.1"
                     /translation="MAGLELRFLGDFEVCREGHAMPLPPSKKTRALLAYLCLQPRRFS
                     RDYLCQLLWERPDDPRGSLRWSLSKIRSLLDDDHTARVQADRNNVGIVLQGLAIDILQ
                     LRHMVQGGLTGLPTDALEAAAAQWRGNVLEGLEFSSFHDFHAWCVAEREQSLRDRTEL
                     LGELARRLADDPTRALPHVRSLVGLSPYDEGHRATLIRLLIAARQFAEAEEQFQLGLR
                     MLKEAGVAASGALVAARRPPRIDAPPLREQPTLETVALVRPAPAAGLYGRETELATLE
                     SALGEVRLHSRCAMLLLSGAPGMGKSSLLAAARQHAVEQGALLLQAAAFEADSIRPFA
                     LWLDALRALDPALHDRVFGDVDVANRDRLFSRLSDWIAQAAATRPVVLLFDDVQWCDE
                     S"
     gene            1366..2733
                     /locus_tag="VFV25_05785"
     CDS             1366..2733
                     /locus_tag="VFV25_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY1392863.1"
                     /translation="MHTDARGLPISAHCTQAATAFDHLIVGYLTQRADTPARLKAVLD
                     VDPDFALAHCMKGYFGMLTFKQASVAGAVECADKARALAAGATPREQQHVAALTAWAA
                     GELDRALALWEAILRRHPLDVVAFRLAHFVNFWLGRSQDMVSSVERVIPAWSVDVPGF
                     PSMLACQAFAHEEAGNYLAAEPAGRRAIELDPGDLWAAHAVAHVMESQGRRSEGLLWL
                     NTLSPHWEGSHNLQHHLWWHCALFKLEQGDIAGALDLYDTRFRNLAAPLTVNSPDVYI
                     DVQNAASMLFRLQRLGVEVGERWVELADKAEARIGDCQSAFTLPHWMMALIAADRGAA
                     AQRMVQALIAYGQGPGTMAPIVRDYVLPIAQAQLAHAAGRHSEAVALMRPVLGGMYRL
                     GGSHTQQDVLEQLFLDAARKAGSTADIRLAVERVAGRWPIAPERFVGWLEAAREAALG
                     MRPIP"
BASE COUNT          498 a         1078 c         1021 g          481 t
ORIGIN      
        1 ctggactcgt cgcaccactg cacatcgtcg aagagcagca ccaccggccg ggtggccgcc
       61 gcctgggcta tccagtcgct gagccggctg aacaggcggt cccggttggc cacatcgacg
      121 tcgccgaaga cacggtcgtg cagcgcagga tccagcgcgc gcagcgcatc cagccacaac
      181 gcgaacggtc ggatgctgtc ggcctcgaag gccgccgctt gcagcaacag ggcgccctgt
      241 tcgacggcat gctgccgggc ggccgccagg agactggact tgcccatgcc cggtgcgccg
      301 ctgagcagca gcatggcgca acgcgaatgc agcctgacct cgcccagcgc cgactccagc
      361 gtggcgagtt cggtctctcg cccgtacagc cctgcagcgg gcgccggcct gacgagggcc
      421 accgtctcca aggtgggctg ctcgcgcagc ggcggtgcgt cgatgcgcgg cggccggcgt
      481 gcggccacca gcgcaccgct ggcggccaca cccgcctcct tcagcatgcg caggccgagc
      541 tggaactgct cctcggcctc ggcgaactgt cgcgccgcaa tcagcagccg gatcagcgtg
      601 gcgcggtgcc cctcgtcata gggcgacagg ccgaccagcg agcgcacgtg gggtagggcc
      661 cgcgtagggt catcggccag tctgcgcgcc agttcgccca gcaactcggt gcggtcgcgc
      721 agcgactgtt cgcgctctgc cacgcaccag gcatggaagt cgtggaagct ggaaaactcc
      781 aggccctcca gcacgttgcc gcgccactgc gcggcggcgg cctccagcgc gtcggtgggc
      841 agaccggtca gtccaccttg caccatgtgt cgcaactgca ggatgtcgat agccagcccc
      901 tgcaaaacga tgccgacatt gttgcgatcg gcctgcaccc gcgcggtatg gtcatcgtcc
      961 agcaggctgc ggatcttcga caggctccag cgcagcgagc cgcgcggatc gtccggcctc
     1021 tcccacagca gctggcacag gtaatcgcgg ctgaaacgcc gcggctgcag gcagagatag
     1081 gccagcagcg cgcgtgtctt cttggacggc ggcaagggca tcgcgtgtcc ttcgcggcag
     1141 acctcgaagt cacccagaaa tcgcagttca agccccgcca tcctgcgctc gtccgctcaa
     1201 tggttgcgcc aatatacgac gggtgagacg ccacagaagc gggacttccc ccggagggtg
     1261 gcactctgcg tcgcgaattc cacgctgact tccacgctgg ctacctcggc agcgcgggat
     1321 catgccaccg ccgatccacc gaacctctca acccggagcg acaccatgca caccgatgcc
     1381 cgcggtctgc ccatcagcgc ccactgcacc caagccgcca cggctttcga tcacttgatc
     1441 gtcggctacc tcacccagcg tgccgacacg ccggcgcgct tgaaggcggt gctggatgtc
     1501 gacccggact tcgcgctggc gcactgcatg aagggttact tcggcatgct gaccttcaag
     1561 caggcttctg tggctggcgc cgtggagtgc gccgacaagg cacgcgccct ggccgcaggc
     1621 gccaccccgc gcgagcagca gcacgtggcg gcgctcacgg catgggccgc tggtgaactg
     1681 gaccgtgccc tggcgttgtg ggaagccatc ctgcgccggc acccgctgga cgtggtggct
     1741 tttcgcctgg cccatttcgt gaacttctgg ctgggccgtt cgcaggacat ggtgtcgtcc
     1801 gtcgagcgtg tgataccggc ctggtcggtg gacgttccgg gcttcccgtc catgctggcc
     1861 tgccaggcct ttgcgcacga ggaggccggc aactacctgg ctgccgagcc cgctggccgt
     1921 cgcgccatcg agttggatcc aggcgacctg tgggccgccc atgccgtggc gcacgtgatg
     1981 gagtcgcaag gccgtcgcag cgaaggtttg ctgtggctga acacgctgtc accgcactgg
     2041 gaaggcagtc acaacctgca gcaccacctg tggtggcact gcgcgctgtt caaattggag
     2101 caaggtgata tcgccggcgc gctggacctg tacgatacgc gctttcgcaa cctggcggca
     2161 ccgctgacgg tgaactcgcc cgacgtctac atcgacgtgc agaacgccgc ttccatgctc
     2221 tttcgcctgc agcgactggg ggtggaagtc ggtgagcgct gggtggaact ggccgacaag
     2281 gccgaggcgc gcatcggcga ctgccagtcc gccttcacct tgccgcactg gatgatggcg
     2341 ctcatcgccg ccgaccgcgg ggccgccgcg cagcgcatgg tgcaggcctt gatcgcctac
     2401 ggccagggcc cgggcacgat ggcgcctatc gtgcgcgact acgtgctgcc catcgcccag
     2461 gcgcagttgg cgcacgccgc gggacgccat tccgaagccg tggcgctgat gcgaccggtg
     2521 ctgggcggca tgtaccgcct cggcggcagc cacacgcagc aagacgtgct ggagcagctg
     2581 ttcctggacg ccgcgcgcaa ggccggcagc actgcagaca tccgcctcgc cgtcgagcgc
     2641 gtcgccggtc gctggcccat tgccccggaa cgctttgtcg gctggcttga agcggcgcgt
     2701 gaggcagcgc ttggcatgcg gccgattcca tgacaccggc gtttcccatt gccctccaca
     2761 ttgtcagagg tcagcaaact ggtcgcttcg gccaccggtc tccctgatct gatgatgacg
     2821 ggtaccctct gacgagcgtc agcgaagaga acagcgcggc tgttcgagtg accggttcag
     2881 ttaaggcttg tgcaacacct gcccgcaggc agcgagggca cgccgtcacc tgcgcgcgag
     2941 ggcaggtgtc gcacaagcct taactccagc tgacgcagcc ttctcagcga ttccggcgac
     3001 caggcgagcg gccccagccc ccatgcgagg ccggcgcccg caaacgacaa ggcgggccaa
     3061 agagccgacg gcggcgcg
//
LOCUS       DASUCY010000299         6814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_954020, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000299 DASUCY010000000
VERSION     DASUCY010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 6814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6814
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954020"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="VFV25_05790"
     CDS             complement(<1..729)
                     /locus_tag="VFV25_05790"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEY1392864.1"
                     /translation="MFVDSHCHLTFPELKAELPAIRAEMAQASVDRALCICTTLEEFD
                     EVHALATHYDNFWATVGVHPDNEDVREPGLDELVRLAKLPRVVAMGETGLDYYRLNGR
                     SVADMEWQRERFRTHIRAARVAGKPLVIHTRSASEDTIDILRAEGEDGSAGSAGGVFH
                     CFTETREVARAALDLGFYISFSGILTFKNAHELQEVAKEVPLERCLIETDSPYLAPVP
                     HRGKTNRPSYVPHVARRLAELKGCS"
     gene            complement(794..1189)
                     /locus_tag="VFV25_05795"
     CDS             complement(794..1189)
                     /locus_tag="VFV25_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY1392865.1"
                     /translation="MSEPTGRSNSGGSPSASPASRPSVIQLVFREKGALYAAYIPLFA
                     EGGLFVPTTRDYKLGEDIYLLLSLPDDAQRYPVAGKVAWITPANAPGGRTQGVGVRFP
                     ADEKTRLLRSRIEEILGTSLASAKPTQTI"
     gene            complement(1186..2232)
                     /gene="holB"
                     /locus_tag="VFV25_05800"
     CDS             complement(1186..2232)
                     /gene="holB"
                     /locus_tag="VFV25_05800"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEY1392866.1"
                     /translation="MNKPMLPWLHQPLSQALRVQRGHALLVHGPQGVGQFEFALALAQ
                     AWLCEAPAAERADGLACGRCASCHLVSTHGHPDLLVLLPEALREALGYGSGESEAPAD
                     GEGKSKAKPSKEIKVEALRAAVAFAQQTSGRGRAKVVVLHPAERMNAISANTLLKTLE
                     EPPGVARFVLSCGAPENLLPTIRSRCQAVRLGLPPTEEAAAWLREQGLAEAELLLAAA
                     GGQPLAALERAALGVDAATWKRLPAMAARGEAAALAGWPLPVVIDALQKLCHDALAQG
                     QGAAPRYFPADSLPDGKTLGRDVSALTAWAAELRQAARSAEHPWNAGLMLEAHILRAK
                     QAFAGGRAKLHSVT"
     gene            complement(2229..2864)
                     /gene="tmk"
                     /locus_tag="VFV25_05805"
     CDS             complement(2229..2864)
                     /gene="tmk"
                     /locus_tag="VFV25_05805"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEY1392867.1"
                     /translation="MTTRARGRFVSFEGIDGAGKSTHIDAVAQRWRDAGIEVVRTREP
                     GGTPLAERLRELVLHEPMDALTEALLIFAARRDHLQQVIEPALARGAAVLCDRYTDAS
                     FAYQGGGRGFDLQVLATLERWVQQGPQGLRQPDLTLWFDLPASVAAARLSQARSPDRF
                     ESQDEAFFQRVIDGYRARCAAQPERFARIDADQPLPAVAAQIDGALKARGW"
     gene            complement(2861..3859)
                     /gene="mltG"
                     /locus_tag="VFV25_05810"
     CDS             complement(2861..3859)
                     /gene="mltG"
                     /locus_tag="VFV25_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEY1392868.1"
                     /translation="MKVLRTLLVLLILGAAAAAGAAWWWLQQPLPLAASSVELSIEPG
                     TSPRDIARGWVAAGVQVPPWSLYQWFRWSGQSRKIRAGSYEITTGATPRELLRKMVQG
                     DESQATVMLIEGWTFRQFRAELARADALKPTTANMSDAEIMAALGLPGVSPEGRFFPD
                     TYAYSKGSSDLAVLKRALRAMQRRLADAWEQRSPASPLHNPDEALTLASIVEKETGRE
                     ADRSMVGGVFANRLRIGMPLQTDPTVIYGLGERFDGNLHKRDLLADGPYNTYTRAGLP
                     PTPIAMPGKAALLAAVKPADTRALYFVSRGDGSSEFSDTLDAHNRAVNKYQRGQGQ"
     gene            3904..4890
                     /locus_tag="VFV25_05815"
     CDS             3904..4890
                     /locus_tag="VFV25_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HEY1392869.1"
                     /translation="MDPSFSTSISFDGAVQLGHGGVIRAVGEDAAGFLNSQLSNDFAR
                     LGLDRGLLAAYCSPKGRMLASFVALRRKPQETSQEVWLATDAELLAPTLKRLGMFVLR
                     AKVKLSDASADLKLVGLTGATAATVSGEAASLLVWGKQDLGAASLLRLPDAGLASGPC
                     PRWLWIGPAAEAESLLASQATLPLAHWQWLEVMSGVAMINAATVEQFVPQMLNYELVG
                     GVDFQKGCYPGQEIVARSQYRGSIKRRTVLVHAEAELKPGVEVYWSLDAGQPAGLVAQ
                     AAHKPLGGGSALVEIKLAALEAGSLHLGSPEGPRLTLGHLPYNLPMDTAAEA"
     gene            4927..5238
                     /locus_tag="VFV25_05820"
     CDS             4927..5238
                     /locus_tag="VFV25_05820"
                     /inference="COORDINATES: protein motif:HMM:NF027614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4936 family protein"
                     /protein_id="HEY1392870.1"
                     /translation="MPELYIYYRVPAGNESAALAATLAMQKLLRERHPQLQARLLRRP
                     ETAADGLQTWMEAYALPGRGIDASLRQEIEAAALTWAALIDGPRHCEVFEVFEVCEPC
                     A"
     gene            5229..6056
                     /locus_tag="VFV25_05825"
     CDS             5229..6056
                     /locus_tag="VFV25_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HEY1392871.1"
                     /translation="MCLVALAIDQHRRFPLVVVANRDEYFARPASTMAWWSQGPGLPA
                     VLSGRDLQSGGTWLGLTTAGRLALLTNVRNPARNDPEAPSRGAIVPLWLRGDLSMDRF
                     WTRVALGGYNGFNLIAMDFARGEGFWATNTQGLPRRLERGIHGVSNAMLDTPWPKVVR
                     LKHRLAVALETSTSTDALAERLFDALADRSLAADEELPSTGISLELERLLSAAFIRTP
                     DGRYGTRCSTLVITERIDRRLVTHVFERSFGAAESDPTQLQHSLLKDWPPQLAMENV"
     gene            complement(6053..6541)
                     /gene="ispF"
                     /locus_tag="VFV25_05830"
     CDS             complement(6053..6541)
                     /gene="ispF"
                     /locus_tag="VFV25_05830"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEY1392872.1"
                     /translation="MNPLNIRIGEGWDTHALVTGRPLILGGVSIPHTHGLLGHSDADA
                     LLHAITDALLGAAALGDIGRHFPDTDAQFKGADSSALLAEAARRVRAAGWEIGNVDST
                     IVAQAPKMAPHINAMCERIAAALGVAVDQVNVKAKTAEKMGPVGEGRAIEARAICLLV
                     RP"
     gene            complement(6577..>6814)
                     /locus_tag="VFV25_05835"
     CDS             complement(6577..>6814)
                     /locus_tag="VFV25_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEY1392873.1"
                     /translation="RALHEAPEFIPGYYDPADPRVLEEGMVITIEPFLSTRSRQAEEA
                     GDGWTLVGGRGNLSAQFEHTLIVTRGEPIVVTRH"
BASE COUNT         1034 a         2327 c         2357 g         1096 t
ORIGIN      
        1 gctgcagcct ttcaactcag ccagtcggcg ggccacgtgg ggcacatagg agggcctgtt
       61 ggtcttgccg cgatggggca ccggcgccag ataggggctg tcggtttcga tcaggcatcg
      121 ctccagcggg acctccttcg ccacctcctg cagttcgtgg gcgttcttga aggtcaggat
      181 gcccgagaag gagatgtaga agccgaggtc cagcgcggcc cgggcgacct cgcgcgtctc
      241 ggtgaagcaa tggaagactc cgcctgccga gcccgcgctg ccgtcctcgc cctcggccct
      301 gaggatgtca atcgtgtcct cgctggcgct gcgcgtgtgg atcacgagcg gcttgcccgc
      361 cacgcgggcg gcgcggatat gcgtgcgaaa gcgctcgcgc tgccattcca tgtcggccac
      421 gctgcggccg ttgagccggt aatagtccag ccctgtttcg cccatggcca cgacgcgcgg
      481 aagcttcgca agccgcacca gttcatccag gccgggttcg cgcacgtctt cgttgtcggg
      541 atgcacgccc accgtggccc agaagttgtc gtaatgcgtc gccagcgcat gcacctcgtc
      601 gaactcctcc agcgtcgtgc agatgcacag cgcgcggtcg acactagcct gcgccatctc
      661 ggcgcgtatg gccggcagct cggccttgag ttcggggaag gtcaggtggc agtgggaatc
      721 gacgaacatg gcgaagcaga agagcccggc tgccgatgca ttgcacgcaa ggcaggcgag
      781 ctggcgaagc agatcagatg gtttgcgtcg gcttggccga ggcgagcgag gtgcccagga
      841 tttcctcgat gcgcgagcgc agcagccgcg ttttctcgtc ggcagggaag cgcacgccga
      901 cgccctgggt acgcccgccg ggggcgttgg ccggcgtgat ccaggcgacc ttgccggcca
      961 ccgggtagcg ctgcgcatcg tcgggcaggg acagcagcag atagatgtcc tcgcccagct
     1021 tgtagtcgcg cgtcgtcgga acgaacagcc cgccctctgc gaacaacgga atgtaggcgg
     1081 cgtacaaggc ccccttttcg cgaaagacca gctgaatgac gctgggccgc gatgccggtg
     1141 aggcagatgg ggagccgccg ctgttgctgc gacctgtggg ttcgctcatg tcaccgagtg
     1201 tagctttgcg cgcccgcctg caaaggcctg tttagccctg agtatgtggg cttccagcat
     1261 caatccggcg ttccaggggt gttcggcaga gcgcgccgcc tggcgcagct cggcggccca
     1321 ggccgtcaga gccgagacat cgcggcccag cgtcttgccg tccggcagac tgtcagccgg
     1381 aaagtagcgc ggcgcggccc cctgcccttg ggccagcgcg tcatggcaga gcttttgcag
     1441 cgcatcgatc accacgggaa gcggccagcc ggcaagagcc gccgcttcgc ccctggctgc
     1501 catggccggc aggcgcttcc aggtggcggc atccacgccc agggccgcgc gctccagggc
     1561 ggccagcggc tggccgccag cggccgccag caacagctcg gcttcggcca agccctgttc
     1621 gcgcagccag gccgcggcct cttcagtcgg cggcaggccc agacgcaccg cctgacagcg
     1681 gctgcgtatg gtcggcaaga ggttctcggg cgcgccgcag ctgaggacga agcgcgcgac
     1741 gccgggcggc tcctccaggg tcttgagcag ggtgttggcc gagatggcgt tcatgcgctc
     1801 ggccggatgc agcaccacca ccttggcccg gccgcgcccg ctcgtctgct gggcaaaggc
     1861 cacggcggcg cgcagcgctt cgaccttgat ctccttgctc ggcttggcct tggacttgcc
     1921 ctcgccgtcg gccggcgctt cggactcgcc gctgccatag cccaaggcct cgcgtagcgc
     1981 ctcgggcagc agcaccagca gatctggatg gccatgcgtc gacacaagat ggcagctcgc
     2041 gcaacgtccg caggccaggc catcggcccg ctcggcggcc ggcgcctcgc agagccaggc
     2101 ctgggcgagc gccagcgcga actcgaattg cccgacgccc tgcggaccat ggacgagcag
     2161 cgcatgcccg cgctgcacgc gcaaggcctg gctcagaggc tggtgcagcc agggcagcat
     2221 gggcttgttc accagccgcg cgccttcaat gcgccgtcga tttgcgccgc caccgcgggg
     2281 agcggctggt cggcatcgat gcgggcgaag cgctccggct gcgccgcgca gcgcgcgcga
     2341 tagccatcga tgacgcgctg gaagaaggcc tcgtcctggc tctcgaagcg atcaggcgag
     2401 cgtgcctgcg acaggcgggc cgccgccacc gaggccggca agtcgaacca cagcgtcaag
     2461 tcgggctgcc tgaggccctg cggcccctgc tgcacccagc gctccagcgt ggcgagcacc
     2521 tgcaggtcga agccgcggcc gccgccctgg taggcgaagc tcgcgtcggt gtagcggtcg
     2581 cacagcacgg cggcgccgcg ggcgagcgcg ggttcgatga cctgctgcag atggtcgcgc
     2641 cgcgccgcga agatcagcag ggcttcggtc agcgcatcca tgggctcatg cagcaccagc
     2701 tcacgcaggc gctcggccag cggcgtgccg cccggctcgc gggtgcgcac gacctcgatc
     2761 ccggcatcgc gccagcgctg cgccaccgca tcgatgtgag tggacttgcc ggcgccgtct
     2821 atgccttcga agctgacgaa gcgtcctcgg gcacgtgtcg tcactggccc tgccctcgct
     2881 ggtacttgtt caccgcgcga ttatgggcgt ccagcgtgtc gctgaattcg ctgctgccgt
     2941 cgccgcggga cacgaagtac aaggccctgg tgtcggccgg cttcaccgcg gccagcaggg
     3001 ccgccttgcc gggcatcgcg atcggcgtgg gcggcaggcc ggcgcgggtg taggtgttgt
     3061 aagggccgtc ggccaacagg tcgcgcttgt gcaaattgcc gtcgaaacgc tcgcccaggc
     3121 cgtagatcac ggtcgggtcg gtctgcagcg gcatgccgat gcgcagccgg ttagcgaaca
     3181 cgccgcccac catggagcga tcggcttcgc ggccggtttc cttctcgacg atcgaagcca
     3241 gggtcaaggc ttcgtcaggg ttgtgcaagg gcgacgccgg gctgcgctgc tcccaggcgt
     3301 cggccaggcg gcgctgcatg gcgcgcagcg cgcgcttgag cacggcgagg tcgctgctgc
     3361 ccttgctgta ggcataggtg tcgggaaaga agcggccctc ggggctgacg ccgggcaggc
     3421 ccagcgccgc catgatctcg gcatcgctca tattggccgt ggtcggcttg agggcatcgg
     3481 cgcgcgccag ctcggcgcgg aactggcgga aggtccagcc ctcgatcagc atcacggtcg
     3541 cctggctctc gtcgccttgc accatcttgc gcagcagctc gcgcggcgtg gcgccggtgg
     3601 tgatttcata gctgccggcg cggatcttgc gcgactggcc cgaccaccgg aaccattgat
     3661 aaaggctcca gggcggcacc tgcacgccag ccgccaccca gccgcgcgcg atgtcgcgcg
     3721 gcgaggtgcc gggttcgatc gacaactcca cgctggacgc ggccagaggc agtggctgct
     3781 gcagccacca ccaggcggcg ccggcggccg cggccgcgcc caggatcaac aacaccagca
     3841 atgtgcgtag aaccttcatg cccctaccaa gcttttgaac atggcgctga tgataatgag
     3901 gccatggacc cctccttctc gacctcgatt tccttcgatg gtgccgtgca gctcggccat
     3961 ggcggcgtca tacgcgccgt cggcgaggac gcggccggtt ttctcaacag ccagctgagc
     4021 aacgacttcg cccgcctcgg gctcgatcgg gggcttcttg cagcctattg ctcgcccaag
     4081 ggccgcatgc tggcgagctt tgtggccctg cgacgcaaac cgcaggaaac ctcacaggaa
     4141 gtctggctgg ccaccgatgc cgagctcctg gcccccacgc tcaagcgcct gggcatgttc
     4201 gtgctgcggg ccaaggtcaa gctcagcgat gccagcgcgg atctgaagct ggtcggcctg
     4261 acgggcgcca ctgccgccac cgtgagtggc gaggccgcga gcctgctcgt atggggcaag
     4321 caggatctcg gcgctgccag cctgctgcgc ctgcctgatg ccggtctcgc cagcggccct
     4381 tgcccgcgtt ggctttggat cggaccggcc gctgaagccg aatccctgct cgcttcgcaa
     4441 gccacgctgc ccttggccca ttggcaatgg ctggaagtga tgagcggcgt ggccatgatc
     4501 aacgctgcga cggtcgagca attcgtgccg cagatgctca actacgagct ggtgggcggc
     4561 gtggacttcc agaagggctg ctatccgggc caggagatcg tggcgcgcag ccagtatcgc
     4621 ggcagcatca agcggcgcac ggtgctcgtg cacgccgaag ccgagctcaa gcctggcgtg
     4681 gaggtgtatt ggagcctcga cgccggccag cccgccggcc tggtcgcgca ggccgcgcac
     4741 aagccgcttg gcggcggcag cgccctggtc gagatcaagc tggccgcgct ggaagcgggc
     4801 tcgctgcacc tgggttcgcc cgaagggccg cggctcaccc tgggccacct gccctacaac
     4861 ttgccgatgg atacggcagc ggaagcttga agctgcctgg ccatccacgg cagcgcggag
     4921 ggcgccgtgc ctgagctcta catctactac cgcgtgcccg ccggcaatga aagcgctgcg
     4981 ctggcagcga ccctagccat gcaaaagctc ttgcgcgagc ggcatccgca gctgcaggcg
     5041 cgcctgctgc gtcgccccga gactgcggcc gacggcttgc agacctggat ggaagcctat
     5101 gccctgccgg gccgcggcat tgacgcctcg ctgcggcagg agatcgaggc agccgccctt
     5161 acctgggctg cgctgatcga cgggccgcgc cattgcgagg tattcgaggt attcgaggtc
     5221 tgcgagccat gtgcctagtc gccctggcga tcgaccagca ccgccgcttt ccgctggtgg
     5281 tggtggccaa tcgcgacgag tacttcgcgc ggccggcctc gaccatggcc tggtggtctc
     5341 aggggccagg cctgcccgca gtgctgagcg ggcgcgacct ccagtccggc ggcacctggc
     5401 tgggcctcac gacggccggc cggctggcgc tgctgaccaa tgtgcgcaac ccggctcgca
     5461 acgatcccga ggcgccgtcg cgcggtgcca tcgtgccgct gtggctgcgc ggcgacctga
     5521 gcatggatcg cttctggaca cgcgtggcgc tgggcggcta caacggcttc aacctcatcg
     5581 ccatggattt cgcgcgcggc gaaggcttct gggccaccaa cactcaaggc ctgccgcggc
     5641 gcctggagcg cggcatccat ggcgtgtcca atgccatgct cgatacgccc tggcccaagg
     5701 tggtccggct caagcaccgc ctggcggtgg ccttggagac gagcacctcg accgatgccc
     5761 tggccgagcg attgttcgat gcgctggcag atcgcagcct ggccgctgac gaggagctgc
     5821 ccagcaccgg catctcgctg gagctggaac gcctgctgtc cgcggccttc atacgcacgc
     5881 cggacgggcg ctatggcaca cgctgctcca cgctggtgat caccgagcgc atcgaccggc
     5941 gcctggtcac gcatgtcttc gagcgcagct tcggtgcggc cgaatcggac cccacacagt
     6001 tgcagcactc gctgctgaag gactggccgc cgcagctagc catggagaac gtctagggcc
     6061 tgaccagcag gcagatggcg cgcgcctcga tcgcgcggcc ctcgcccacc gggcccatct
     6121 tctcggccgt tttcgccttc acgttcactt gatcgacggc gacgcccagc gcggcggcga
     6181 tgcgctcgca catcgcattg atgtgcggcg ccatcttggg cgcctgggcc acgatggtgg
     6241 agtccacgtt gccgatctcc cagccggctg cacgcacgcg cctggcggcc tcggccagca
     6301 gcgccgatga atcggcgccc ttgaactgcg catcggtgtc cgggaaatgc cggccgatgt
     6361 cgcccagcgc agctgcgccg agcaaggcgt ccgtgatggc gtgcagcagc gcatccgcat
     6421 ccgaatggcc gagcaggccg tgcgtgtgcg ggatgctcac gccgccgagg atcaaggggc
     6481 ggccggtgac gagggcatgg gtgtcccagc cttcgccgat gcggatgttc aagggattca
     6541 tgcgttcagt gtgaagtgcg ccgttgcttg ctcggttcag tgtcgcgtga cgacgatggg
     6601 ctcgcctcgc gtgacgatca aggtgtgctc gaactgggcc gatagattgc cccggccgcc
     6661 caccagcgtc cagccatcac cagcttcttc ggcttgtcgg ctgcgggtgg acaggaaggg
     6721 ttcgatggtg atcaccatgc cctcttcgag cacgcgaggg tccgccggat cgtagtagcc
     6781 gggaatgaac tcgggcgcct catgcaaggc gcga
//
LOCUS       DASUCY010000300         4537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1066424, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000300 DASUCY010000000
VERSION     DASUCY010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066424"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..1243)
                     /locus_tag="VFV25_05840"
     CDS             complement(53..1243)
                     /locus_tag="VFV25_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY1392874.1"
                     /translation="MSTRAKQNKTHNRPSTGASRQGSHGTPAATLLAGILALSFAAGA
                     AQAAEKNGEPAPALLVRKGEQLLVPADSPLRARLVVAPVAAQALPHGLAIPAVVEADP
                     ALNVNVLPPLTGRVVELKAGLGDVVKRGQLLAVISSPDLAQAYADVDKAKDTLSLTRR
                     ALERAQGVNEAGGAAQKDLEAAQSAYNQALAEDQRAASRLKTLAGGGEVDMRSRLLRI
                     NAPVAGTVTSLAVGNGAFINDPTAPLMSIANLERVWVTAQVPEDQIGAITRGQAVELR
                     LAAYPGLSLRGTVGSVSPLLEPDTRRAKVRITLANPDGRLKPNMFATARFAVPQAAQV
                     VVPTSALLMNNDSVTVFVESAPWTFVRRTVELGTEDGERVRILSGLKSGERVVVRGGV
                     LLND"
     gene            complement(1431..2357)
                     /locus_tag="VFV25_05845"
     CDS             complement(1431..2357)
                     /locus_tag="VFV25_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY1392875.1"
                     /translation="MLTSMPRDPRLPLQNLAAFRAAARLQNLRAAAEELNLTHSAVSQ
                     QIRALEEQLGFEVFHRHGRRIALNAAGEALMQGVERAWVHLDEALHAAAAAHGGDETR
                     LRLTTLPSFAQRWLMPRIARWREHYPDIALELHTSPQVVDLQRKGYHAAVRQGRGPWA
                     GLDGERLADSAFIAVGAPSTARRLLGAPEAALADEPLLGDVDSWTQWFTAGGMRKRIV
                     PVASFNDAGLMLQAAEQGLGIALARELLAADALAEGRLMRLSPRTCVLEGAYSYHLVY
                     PPHLRDWPPLAALRDWLFDEMERSRRTLSTVS"
     gene            2453..2755
                     /locus_tag="VFV25_05850"
     CDS             2453..2755
                     /locus_tag="VFV25_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392876.1"
                     /translation="MSSTSLIRTIPLSRSLSQRAVQAISEALLEAQAWWLAVAQRRRR
                     AREIDLATELDQRLLEDIGLKGCERAEITARREWDAQRNGELRLGMGLSVFDGRHW"
     gene            2804..3229
                     /locus_tag="VFV25_05855"
     CDS             2804..3229
                     /locus_tag="VFV25_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HEY1392877.1"
                     /translation="MTAVSKTPASQDKQAASAYDWVGGEAAVRALVDRFYDLMDLESA
                     YAELRALHPTTLEGSRDKLFWFLCGWLGGPNHYVDRFGHPMLRARHLPFAIGIKERDQ
                     WLACMDQAMHEVKLDPALITRLREAFFGTADWMRNRGGA"
     gene            complement(3242..3832)
                     /locus_tag="VFV25_05860"
     CDS             complement(3242..3832)
                     /locus_tag="VFV25_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEY1392878.1"
                     /translation="MQQATQEAARREAEQRERQAAERRERELFATSVGPVTPLKPATR
                     AAVHRPKPEPQPLQRRLDEEAVLRASLSDEFDVESLLETDETLSFRRPGLGPDVVRKL
                     RRGVWVIQGQIDLHGLRREEARESLASFLHEAVKQGLRCVRIVHGKGHGSPGREPVLK
                     SKVRAWLVQKAEVLAFVQARAAEGGAGALVVLLRAG"
     gene            complement(3887..>4537)
                     /gene="radC"
                     /locus_tag="VFV25_05865"
     CDS             complement(3887..>4537)
                     /gene="radC"
                     /locus_tag="VFV25_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEY1392879.1"
                     /translation="ELRPREKLLERGPEALADAELLALLLRTGYQGVGVLSLAQRLLD
                     EFGGIPGLLRATPEDLARIKGLGPAKRAELSAVLELSRRALATQLAERPIFESPQTVK
                     DYLLLHLSDRAHEVFSVLFLDARHRLQAFEELFRGTLTQTSVYPREIVKRALALNAAA
                     VVLAHNHPSGVAEPSKADEYLTATLKSALALIDVRVLDHLVVGRGEVVSFAERGLI"
BASE COUNT          662 a         1565 c         1614 g          696 t
ORIGIN      
        1 cgtcagcagc cagaccaggt ggcggcgccg gaagcacagc gcgatgaact ggttaatcat
       61 tcagcagcac cccgccgcgg accacgacgc gctcgccgct cttgaggccg gacaggatgc
      121 gcacgcgctc gccgtcctcg gtgccgagct cgaccgtgcg ccgcacgaac gtccaaggcg
      181 cgctctcgac gaagaccgtc acgctgtcgt tgttcatcag cagggccgag gtcggcacga
      241 ctacctgcgc cgcctggggc acggcgaatc gcgcggtggc gaacatgttg ggcttgaggc
      301 gcccgtccgg gttggccagg gtgatgcgca ccttggcgcg ccgtgtgtcc ggttctagca
      361 gcgggctcac cgagccgacc gtgccgcgca ggctcaggcc ggggtaggcg gccaggcgca
      421 gctccacggc ctgaccgcgg gtgatggcgc cgatctgatc ctccggcacc tgggccgtga
      481 cccacacgcg ctcgaggttg gcgatgctca tcaagggcgc ggtcgggtcg ttgatgaagg
      541 cgccgttgcc cacggccagg ctcgtcaccg tgccggccac cggcgcgttg atgcgcagca
      601 ggcggctgcg catatcgacc tcgccgccgc cggcgagcgt cttcaggcgc gaggcggcgc
      661 gctggtcttc ggccagcgct tggttgtagg cgctttgcgc ggcctcgagg tccttctgcg
      721 cagcgccgcc cgcctcgttg acgccttgcg cgcgctccag cgcgcggcga gtgaggctca
      781 gcgtgtcctt ggccttgtcg acatccgcat aggcctgggc caggtcgggc gagctgatca
      841 cggccagcag ctggccgcgc ttgaccacgt cgccgaggcc ggccttcagt tcgacgacgc
      901 gaccagtcag cggcggcagc acgttgacgt tcagtgcggg atcggcctcc acgacggcgg
      961 gtattgccag gccgtggggc agggcctggg cggcgaccgg cgccaccacg agccgggcgc
     1021 gcagcggcga atcggccggc acgaggagtt gctcgccctt gcgcaccagc agcgcgggcg
     1081 ccggctcgcc gttcttctcg gcggcctgcg cggcgccggc ggcgaagctc aaggccagga
     1141 tgcccgcaag cagcgtggcg gctggcgtac cgtgtgatcc ctgtcgcgaa gcacccgtgg
     1201 atggcctgtt gtgggtcttg ttctgctttg cgcgggtcga catggggagc actccggaat
     1261 ctgccgggtc gagggccgag gccctctttg ttgatgcggg gatgggcggc gattgagcga
     1321 gcactgcgcc gcgcgggcac aggctgccaa gccctgtggt tcggaagccg cgattgtcgg
     1381 ggatggtccc gggcgtcaga tgacaaaaac gtgacggacg ggccagcgcc tcaggacacc
     1441 gtgctcaagg tccggcgtga gcgctccatc tcgtcgaaca accagtcgcg cagtgccgcc
     1501 agcggcggcc aatcgcgcag atggggcgga tagacgaggt ggtagctgta ggcgccttcg
     1561 agcacgcagg tccgcggcga cagccgcatc aggcggccct cggccagggc atcggccgcg
     1621 agcagttcgc gcgccagcgc gatgcccagg ccctgctcgg ccgcctgcag catcagcccc
     1681 gcatcgttga agctcgccac tggaacgatg cgcttgcgca tgcccccggc ggtgaaccac
     1741 tgggtccacg agtccacgtc gcccagcaag ggttcgtcgg ccagggccgc ttcgggcgcg
     1801 ccgagcaggc ggcgcgcggt cgagggcgcg ccgacggcga tgaaggccga gtcggccagc
     1861 cgctcgccgt ccaggcctgc ccagggcccg cgcccctggc gcaccgcggc gtgatagccc
     1921 ttgcgctgca gatcgacgac ctggggcgag gtgtgcagct ccagcgcgat gtccggatag
     1981 tgttcgcgcc agcgcgcgat gcgcggcatc agccagcgct gggcgaagga gggcagggtc
     2041 gtgaggcgca accgcgtctc gtcgccgcca tgcgcggccg ccgcggcatg cagggcctcg
     2101 tccagatgca cccaggcccg ttccacgcct tgcatcaagg cttcgccggc ggcgttcagc
     2161 gcgatgcgcc gtccgtggcg gtgaaagacc tcgaagccca gctgctcctc cagcgcgcgt
     2221 atttgctggc tgacggcgct gtgcgtgaga ttgagctcct cggcggcggc gcgcaggttc
     2281 tgcaggcggg ccgcggcgcg gaaggcggcc aggttctgta gcggcaggcg tggatcgcgg
     2341 ggcatgctgg taagcataac tgcccaagct agccagaaca agtcgcttct ttcgcgaagg
     2401 ttgggtagat atgctttcca acctatcgaa cgatcttcaa ggagccagca tcatgtccag
     2461 cacttctttg attcgcacca tcccgctgtc gcgctccctg tcccagcgcg ccgtacaggc
     2521 tatctcggaa gccttgctcg aggcgcaggc ctggtggctc gccgtcgccc aacgccggcg
     2581 ccgggcgcgc gagatcgatc ttgcgaccga gctggatcag cgcctgctcg aagacatcgg
     2641 tctcaagggc tgcgagcgcg ccgagatcac cgcccggcgc gagtgggacg cgcagcgcaa
     2701 cggcgagctg cgcctgggca tgggcctgag cgtgttcgac gggcgtcact ggtgaaggcg
     2761 tttgtgccgc ggcggctatg cttggcgttt cgccaccagc cgcatgaccg ccgtgagcaa
     2821 gacgcccgcc agccaggaca agcaagccgc cagcgcctat gactgggtgg gtggcgaggc
     2881 ggcagtccgc gccctggtcg accgcttcta cgacctaatg gatctggagt cggcctatgc
     2941 agagctgcgc gccttgcatc ccaccacact ggagggctcg cgcgacaagc tgttttggtt
     3001 cctctgcggc tggctgggcg gccccaacca ctacgtcgac cgcttcggcc accccatgct
     3061 gcgcgcgcgc cacctgccgt ttgctatcgg catcaaggag cgcgaccagt ggctggcctg
     3121 catggaccag gccatgcacg aggtgaagct cgatcccgcg ctgattacgc gcttgcgcga
     3181 ggccttcttc ggtactgcgg actggatgcg taatcgcggc ggagcctgag cgccaggcat
     3241 ctcagccggc ccgaagcagc accaccagcg cacccgcgcc gccctcggcc gcacgggctt
     3301 gcacgaaggc gagcacctcg gccttctgta ccagccaggc gcgcacctta ctcttcagca
     3361 caggctcgcg gccgggcgaa ccatggccct tgccgtgcac gatgcgcacg cagcgcaggc
     3421 cctgcttcac cgcctcgtgc aggaagctag ccaggctttc gcgcgcctcc tcgcggcgca
     3481 gcccgtgcag gtcgatctgg ccctggatga cccagacgcc gcggcgcagc ttgcgcacca
     3541 cgtccggccc caggcccggg cggcggaacg acagggtctc gtcggtctcg agcagggatt
     3601 cgacgtcgaa ctcgtccgac aggctggcgc gcagcacggc ttcctcgtcg agccgtcgct
     3661 gcaagggctg cggctcgggc ttggggcggt ggacggcggc ccgcgtggcg ggcttgagcg
     3721 gcgtcaccgg ccccacgctg gtcgcgaaaa gctcacgctc gcggcgctcg gcggcttggc
     3781 gttcgcgctg ctcggcctcg cgtctggcag cttcctgcgt ggcctgctgc attgcgcgcc
     3841 gcacggcgtc gagatcctgc aggctcttga gcttcacggc gcgacctcag atcagcccgc
     3901 gttcggcgaa ggaaactact tcgccgcggc cgaccaccag gtgatcgagc acgcgcacat
     3961 cgatcagcgc cagcgccgat ttcagcgttg cggtcaggta ttcatcagcc ttggacggct
     4021 cggcgacgcc ggaggggtgg ttgtgggcca gcaccacggc ggcggcgttg agcgccaggg
     4081 cgcgcttgac gatctcgcgc gggtagacac tggtctgcgt cagcgtgccg cgaaacagct
     4141 cctcgaaggc ctgcagccgg tggcgggcat cgagaaatag caccgagaag acttcatggg
     4201 cccggtccga cagatgcagc agcagatagt ccttcaccgt ctgcggcgac tcgaagatgg
     4261 ggcgctcggc cagctgggtg gccagggcgc ggcgcgacag ctccagcacg gccgacagct
     4321 cggcgcgctt ggccggcccc aggcccttga tgcgcgcgag gtcctcgggc gtggcccgca
     4381 gcaagccggg gatgccaccg aattcgtcca gcagccgctg ggccaggctc agcacgccca
     4441 cgccctggta gcccgtgcgc agcagcagcg ccagcagttc ggcatcggcc agggcctcgg
     4501 ggccgcgctc cagcagcttc tcgcgcgggc gcagttc
//
LOCUS       DASUCY010000301         4536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_391857, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000301 DASUCY010000000
VERSION     DASUCY010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4536
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391857"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1733)
                     /locus_tag="VFV25_05870"
     CDS             complement(<1..1733)
                     /locus_tag="VFV25_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392880.1"
                     /translation="MSVSSRLVMQAVGVAAAAGALGAGLIACGSGSGSDSATVAVQGD
                     VAIAYVMRSTALSMNPTNGAPTAPGGDLFIREKSSPSAPEHNITAQFTQGVGDVSDPE
                     VSYDGKKIVFSMTCPTSNTATIAGQPACTGRWSIWEYDMSSGGLTGGSFRRITSASQA
                     DDVDPAYLPGGAGFIFSSNRQTESAPNQALGQSYYALDEYERERVFNLHTMAADGSNI
                     TQISFNQSHDRNPVVRPNGDIMFSRWDHLGGRNHFKVFRVKPDGTDLFVLYGAHSPGN
                     SFLHPRDMDPGGKYAGFVASDLMPLSRTQEGGALVFIDAANYSEENTPANATVPTQGG
                     QTQPTAQALNFDTGLSTYGRITTPYPLWDGTNRVLLAYRPCEVTRNGVVVSCAALTSD
                     ELARLSDPNRLAADIAADPIQDNVPAAYAIYMFDPAKQTWLNVATPPSGFMYTDPIPL
                     QPRPEPNATAPTSVDATLAQQHMGRLEVRSVYDTDGLGRMGDPVLSAADLPAGCTTSI
                     AKTTPTDPLDTRAQVADLVSIKDPAKAAYGCAPARFVRAVRAVAPPANTMGLRTAIGE
                     TDFEPQQILGYA"
     gene            complement(1789..2775)
                     /locus_tag="VFV25_05875"
     CDS             complement(1789..2775)
                     /locus_tag="VFV25_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEY1392881.1"
                     /translation="MSFASASLSTTLAAEPRGLQGGWQSREEAIMGTSIKVELWSDDR
                     TAGEAAIDAVMAEMHRIDAAMSPFKPQSELSRINREAAGGPVRIGEEMFALIERSLVF
                     SRLSDGAFDISYAGVGHLYDYRERIKPTDAAIDEARAAVGYRKLILDRRMLSIRFARP
                     GMRIDLGGFAKGHAVDRGAAILKRRGITNAIVTAGGDSRILGDRRGRPWTIGIRDPRT
                     PDGVIAVLPLEDCALSTSGDYERYFDDETGVRCHHVIDPGTGKSPANDIRSVTVIAGD
                     GLTSEALTKTMFVLGLEKGMRLVEAQPDADAVVVDSSGRLHYSAGLTGGDSG"
     gene            complement(3106..>4536)
                     /locus_tag="VFV25_05880"
     CDS             complement(3106..>4536)
                     /locus_tag="VFV25_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEY1392882.1"
                     /translation="AAALPVGVALMAVFAALRLLREARLRQALQAVVAVALIVAAFWL
                     AGSWFDGLGRLNLLIFFVGVAAGCVFAGIPIAFAFGLATFGYLALGTSTPLPVMVGRM
                     DEGMSHLILLAVPLFVFLGLLIEMTGMARAMVAFLASLLGHVRGGLSYVLIGAMYLVS
                     GISGSKAADMAAIAPALFPAMKERGAKEGDLVALLAATGAQTETVPPSLVLITIGSVT
                     GVSIAALFTGGLLPALVLGLVLCAVVRFRSRHEDLGQTQRRPWKEVARLFAVAAPALA
                     LPFVIRAAVVEGVATATEVSTLGIAYAVLAGLLVYRQFDWRRLVPMLVDTAALSGAIL
                     LIIGTATAMAWGLTQSGFSRSLAELMAALPGGQAMFLAVSVLAFTVLGSVLEGIPAVV
                     LFGPLLFPIARQLGVHEVHYAMVVVLSMGLGLFAPPMGVGYYAACAIGRVNPDAGIRP
                     IAGYLLALLLGTIVVAAIPWISIGFL"
BASE COUNT          769 a         1507 c         1566 g          694 t
ORIGIN      
        1 gcatagccga ggatctgctg cggctcgaaa tcggtctcgc cgatcgccgt gcgcaggccc
       61 atggtgttgg ccggcggcgc caccgcgcgc acggcgcgca cgaagcgggc cggcgcgcag
      121 ccgtaggccg ccttggccgg atccttgatg ctgaccaggt ctgccacctg cgctcgcgtg
      181 tccagcgggt cggtcggtgt ggtcttggcg atcgatgtgg tgcagcccgc gggcaggtcg
      241 gcggcgctga gaaccgggtc gcccatgcgc ccgaggccgt cggtgtcgta gacgctgcgc
      301 acttccagcc ggcccatgtg ctgctgggcc agggtcgcat cgacgctggt cggcgcggtt
      361 gcgttcggct cgggccgcgg ctgcagcggg atgggatcgg tgtacatgaa gcccgagggc
      421 ggcgtcgcga cgttcagcca ggtctgcttc gccgggtcga acatgtagat cgcataggcc
      481 gccggcacgt tgtcctggat gggatcggca gcgatgtcgg cggccaggcg gttcggatcg
      541 ctcaagcgcg cgagttcgtc ggacgtcagc gccgcgcagg agacgaccac gccattgcgc
      601 gtcacctcgc agggccgata ggccagcagc acgcgattcg tgccgtccca cagcggataa
      661 ggcgtcgtga tgcgcccgta tgtcgacagg ccggtgtcga agttcagtgc ctgggccgtg
      721 ggctgggtct gtccgccttg ggtgggcaca gtggcattgg ccggagtgtt ctcctcggaa
      781 tagttggccg cgtcgatgaa gaccagcgcc ccgccctcct gggtgcgcga cagcggcatc
      841 aaatcggagg cgacgaagcc ggcatacttg ccgcccgggt ccatgtcccg cggatgcagg
      901 aagctgttgc ccgggctgtg cgcgccatag agcacgaaga gatcggtgcc gtcaggcttg
      961 acgcggaaga ccttgaaatg gttgcgcccg cccaggtgat cccagcgcga gaacatgatg
     1021 tcgccgttgg gccgcaccac cgggttgcgg tcatggctct ggttgaacga gatctgcgtg
     1081 atgttgctgc cgtcggccgc catcgtgtgc aggttgaaga cgcgctcgcg ctcgtattcg
     1141 tccagcgcgt aatagctctg gcccagggcc tggttgggcg cggattcggt ctggcggttg
     1201 gacgagaaga tgaagccggc gccgcccggc aggtaggccg gatcaacatc atcggcctgg
     1261 ctcgcgctcg taatgcgccg gaagctgccg ccggtcagcc cgccgctgct catgtcgtat
     1321 tcccagatgc tccagcgacc ggtgcaggcg ggctggccgg caatcgtcgc agtgttcgac
     1381 gtggggcaag tcatcgagaa gacgatcttc ttgccgtcgt aggacacctc gggatccgac
     1441 acatcgccca cgccctgcgt gaactgcgcg gtgatgttgt gctccggcgc gctgggcgag
     1501 gacttctcgc gtatgaacag gtcgccgccc ggcgcggtgg gcgcgccatt ggtgggattc
     1561 atgctgagcg ccgtggagcg catcacatag gcgatcgcga catcgccctg caccgcgacg
     1621 gtggcggagt cgctgccgga gcccgagccg caggcaatga ggccggcacc cagcgctcct
     1681 gctgcagcgg caacaccgac cgcctgcatc accagccttg atgaaacgct catctggatc
     1741 tcctttggac tttgtctgct tattcgtctc gatccgccgg tcgctgcttc agccgctgtc
     1801 gccgccagtg aggccagccg agtaatgcag ccggccactg ctgtcgacga ccaccgcgtc
     1861 ggcgtccggc tgggcctcga cgagccgcat gcctttttcg aggcccagca cgaacatggt
     1921 cttggtcagc gcctcgctgg tcaggccgtc gccggcaatc accgtcaccg agcggatgtc
     1981 gttggcgggc gacttgccgg tgccgggatc gatcacgtgg tggcagcgca ccccggtctc
     2041 gtcgtcgaaa tagcgctcgt agtctccgga ggtggagagc gcgcagtctt ccagcggcag
     2101 cacggcaatc acgccatcgg gcgtgcgcgg atcgcgtatg ccgatggtcc agggccgccc
     2161 gcggcggtcg ccgaggatgc gactatcgcc gccggccgtg acgattgcat tggtgatgcc
     2221 gcgacgcttg agaatcgccg caccccgatc caccgcatga cccttggcga agccgccgag
     2281 gtcgatgcgc atgcccggcc gcgcgaagcg aatgctgagc atgcggcgat ccaggatcag
     2341 cttgcggtag ccgaccgcgg cgcgcgcctc gtcgatcgcg gcatcggtcg gcttgatgcg
     2401 ctcgcggtag tcgtagagat ggccgacccc ggcatagctg atgtcgaagg cgccgtccga
     2461 caggcgcgag aagacgagcg agcgctcaat cagcgcgaac atctcctcgc cgatgcgcac
     2521 cggcccgccg gcggcctcgc ggttgatgcg cgagagttcg gactgcggct tgaaggggct
     2581 catcgcggcg tcgatgcggt gcatctcggc catcaccgca tcgatggccg cttcgccggc
     2641 cgtgcggtcg tcgctccaca gctcgacctt gatcgaggtg cccatgatgg cttcctcgcg
     2701 gctttgccag ccaccctgaa ggccgcgcgg ctcggcggcg agagtagtgc tgaggcttgc
     2761 cgatgcgaag gacatggcta aacccagcgg cggcacgcag ttagcgtgcc gcccgtcgcc
     2821 atgacaccct gatccaagcc ctccgcatcc actgtggtcc cccggtgctt gtcggttgga
     2881 atttattgac gcgccgcgat tgttcctggc gccgggtcac gcaaatcgca tgcccgtatg
     2941 ggtggcgcat ctcgaggcct gagcggtcgc gaagccgcca aatgttggcc gatgtagcag
     3001 aaccctacat tcagtcgcac acaccgagat gacgagtttt gcgacgcttg cgagcagatg
     3061 cttcaagtgt atgcaagtgc aacacggctc cgtggccaga ggtggctaga ggaatccgat
     3121 cgagatccag gggatcgccg caacgacgat cgtgccgagc agcagcgcca gcagatagcc
     3181 ggcaatgggg cgtatgcccg catcggggtt cacgcggccg atcgcgcagg cggcgtagta
     3241 gcccacaccc atgggtggcg cgaacagccc caggcccatg gacaggacca cgaccatcgc
     3301 gtagtgcact tcgtgcacgc cgagctggcg cgcgattgga aacagcagcg ggccgaacag
     3361 cacgaccgcc ggaatgcctt cgagcaccga gcccagcacc gtgaaggcca gcaccgagac
     3421 ggccaggaac atcgcctggc cgccgggcag cgccgccatg agttcggcca gcgagcgcga
     3481 aaagcccgat tgcgtgagcc cccaggccat cgcggtggcc gtgccgatga tcagcaggat
     3541 ggcgccggac agagcggcgg tgtcgaccag catgggcacg aggcgccgcc agtcgaactg
     3601 ccgatagacc agcaagcccg ccagtacggc ataagcgatg cccagcgtcg agacttcggt
     3661 cgcggtggcg acgccttcga ccaccgcggc gcggatcacg aagggcaagg ccagggccgg
     3721 tgcggccacc gcgaacaacc gcgccacttc cttccagggc cggcgctgtg tctgcccaag
     3781 atcctcatgc cggctgcgaa agcggaccac ggcgcacaag accagcccca gcaccaaggc
     3841 cggcaacagg ccgccggtga acagcgcggc gatagacacg ccggtcaccg agcctatcgt
     3901 gatcagcacc aggctgggcg gcacggtctc ggtctgcgcg ccggtggcgg cgagcaaggc
     3961 cactagatcg ccctccttcg cgccgcgctc cttcatcgcc gggaacaagg ccggcgcgat
     4021 ggccgccatg tcggccgcct tggagcctga gatgcccgac acgaggtaca tcgcgccgat
     4081 cagcacatag gacaggccgc cccgcacatg gccgagcagg ctggccagga aggcgaccat
     4141 cgcgcgggcc atgccggtca tctcgatcag gaggccgagg aagacgaaga gcggtaccgc
     4201 cagcaggatc aagtgcgaca tgccctcgtc catgcggccg accattaccg gcagcggcgt
     4261 gctggtgccc agcgccagat agccgaaggt cgcgaggccg aaggcgaagg cgatcgggat
     4321 gccggcgaac acgcaacccg ccgcgacgcc gacgaagaag atcagcaggt tgagccgccc
     4381 gagcccatcg aaccaggagc ccgcgagcca gaaggccgcc acgatgagcg cgaccgcgac
     4441 caccgcctgc agcgcctgcc tcagcctcgc ctcgcgcagc aggcgcagcg ccgcgaagac
     4501 tgccatcagc gccacgccca ccggcagcgc ggcggc
//
LOCUS       DASUCY010000302         2081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1596608, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000302 DASUCY010000000
VERSION     DASUCY010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1596608"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="VFV25_05885"
     CDS             complement(<1..573)
                     /locus_tag="VFV25_05885"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY1392883.1"
                     /translation="MKTITTLVAALSLAAGAAHAASADPLSGTLRIGGSDQMNSLLAH
                     WEQGFAKLHPNVHFAHWLHGSGTGMYGLEMRTTDIALMGRPVNPYERYGIYERAWVYP
                     LEIEVATGAVSQPHKSAAYAIFVNKSNPLARLTMQQLDGIFGAERGGGWKALTWDEGA
                     AHTPAQNIRTWGQLGLTGDWANKPIHTYGPP"
     gene            complement(576..1643)
                     /locus_tag="VFV25_05890"
     CDS             complement(576..1643)
                     /locus_tag="VFV25_05890"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY1392884.1"
                     /translation="MLTTGSAQAADEYKPAHYDLSALPAYQPEQMALGVVRIYGTPLE
                     SLVGQWASAFRAKQGHVRLNAYLINTSQAFAGLLTDQADIGLMGHRTWHTSVMAFNKA
                     FGYEPLEIRFATGSYDDPQGSTPGLMFVVHKSNPLARLTLEQIDGIFCAQRTGGWDGT
                     KWSTAAARGADKNIRTWGQLGLTGEWADKPIHPHGSDVTLSNWADLIEREACHGGKKW
                     NPALIEGPRADIGLKAHGKTRDQEIIEGVQNDPLAIGFMFQRVINAAHSDVKVLPLAR
                     TAAGPYVAPSAQSFFDGSYPMHNGPYLYLNRKPGQPLSARDKEFVRFVLSREGQQIVA
                     DSRIFIPLNAEQIRAELKKLD"
     gene            complement(1746..>2081)
                     /locus_tag="VFV25_05895"
     CDS             complement(1746..>2081)
                     /locus_tag="VFV25_05895"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY1392885.1"
                     /translation="LMMRDLSNDRYGIAYTGMPFKTPQTKALALAERPGEPYVELTLD
                     NVQARRYPLLRDVYYYLKREKGKPLDPNAREFLRYVLSREGQAAVQRDGKYLPLTAQA
                     AAEQLRKLN"
BASE COUNT          307 a          659 c          716 g          399 t
ORIGIN      
        1 gggcgggccg taggtgtgga tgggcttgtt ggcccaatca ccggtgaggc ccagctggcc
       61 ccaggtgcgg atgttctggg ccggcgtgtg ggccgcgccc tcgtcccagg tcagcgcctt
      121 ccagccgccg ccgcgctcgg cgccgaagat gccgtccagc tgctgcatgg tcagcctggc
      181 cagcggattg ctcttgttga cgaagattgc gtaggccgca gacttgtgcg gctggctcac
      241 ggcgcccgtg gccacttcga tctccagcgg ataaacccag gcgcgctcgt agatgccgta
      301 gcgctcgtag gggttgacgg gccggcccat caacgcgata tcggtggtgc gcatctccag
      361 gccatacatg ccggtgccgc tgccgtgcag ccagtgcgcg aagtgcacgt tcgggtgcag
      421 cttggcaaag ccctgttccc agtgcgccag caggctgttc atctggtcgc tgccgccgat
      481 gcgcagcgtg ccggacaagg ggtcggcgct tgcggcatgg gccgcgccgg ctgcgaggct
      541 gagggccgca acgagggttg ttatggtttt catggtcaat ccagcttctt cagttcggcg
      601 cgaatctgct cggcgttcag cgggatgaag atccggctgt cggccacgat ctgctggccc
      661 tcgcggctca gcacgaagcg cacgaactcc ttgtcgcgcg ccgaaagcgg ctggcccggc
      721 ttgcggttga ggtagaggta gggcccgttg tgcattgggt agctgccgtc gaagaagctc
      781 tgcgcgctcg gcgctacgta cggcccggct gccgtccgcg cgagcggcag caccttcaca
      841 tcggagtggg ctgcgttgat gacgcgctgg aacatgaagc cgatcgccaa tggatcgttc
      901 tgaacccctt cgatgatttc ctggtcgcgc gtcttgccgt gggccttcag gccgatgtcg
      961 gcgcgcgggc cttcgatcag cgccgggttc cacttcttgc cgccgtggca ggcctcgcgc
     1021 tcgatcaggt cggcccagtt gctcagcgtg acgtcggagc cgtgcggatg gataggcttg
     1081 tcggcccact ctccggtcaa gcccagctgg ccccaggtgc gaatattctt gtcggcgccg
     1141 cgcgccgcgg cggtggacca tttggtcccg tcccagccgc ccgtgcgctg ggcgcagaag
     1201 atgccgtcga tctgctccag cgtcagccgc gcgagcggat tgctcttgtg aacgacgaac
     1261 atcaagcccg gcgtcgaacc ctggggatcg tcgtaggaac ccgtcgcaaa gcggatctcc
     1321 agcggctcgt agccgaaagc cttgttgaag gccatcacgc tggtgtgcca ggtacggtgg
     1381 cccatcaggc cgatatcggc ctggtcggtc aacaggccgg cgaacgcctg cgacgtgttg
     1441 atgagatagg cgttcaagcg cacatgcccc tgcttggcgc gaaacgcact ggcccattgg
     1501 cctaccagcg attccagcgg cgtgccgtag atgcgcacga cgcccagcgc catctgctcg
     1561 ggctggtagg cgggcaaggc gctcaggtca tagtgcgccg gcttgtactc gtccgcggcc
     1621 tgcgcactcc ccgtggtgag catgcaggca gtgagagcag ccgccagtgc gcatcggctg
     1681 attgccttgc aaagggtgat gtgattcatg atttttgagt cctggtagac atggggcggc
     1741 cgcgatcagt tcagcttcct gagttgttcc gccgcggcct gtgccgtcag cggcaggtac
     1801 ttgccgtcgc gctgcacggc agcctggccc tcgcggctga gcacatagcg caggaactcc
     1861 cgcgcattcg gatcgagagg cttgcccttc tcgcgcttca ggtagtagta gacgtcgcgc
     1921 agcagcgggt agcgccgcgc ctgcacgttg tccagcgtca gctcgacata cggctcaccg
     1981 ggccgctcgg ccagcgcgag ggccttggtc tgcggcgtct tgaacggcat gccggtgtag
     2041 gcgatgccgt agcggtcgtt cgacaggtcg cgcatcatca g
//
LOCUS       DASUCY010000303         2029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1371969, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000303 DASUCY010000000
VERSION     DASUCY010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1371969"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /locus_tag="VFV25_05900"
     CDS             <1..987
                     /locus_tag="VFV25_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AmpG family muropeptide MFS transporter"
                     /protein_id="HEY1392886.1"
                     /translation="ADGLVAIGAFALLVAFASATQDVAIDAWRIEAADDAQEVGLLTS
                     AAQLGYRIAMLVTDAAIIAAAGRIGWPMSYVAMAVLMGVGIAATFFAFEPLRADQVLH
                     DKPPLWSRRGLVDAMIGPFVDFFAKHKAVGLVILLAVALYRLPDFIMGPMYNPYYHDL
                     GIAKDTVALVRGSFGLAATLVGLAAGGVMSVRVGLLPTLVAGLALEALGTGSFALLAL
                     HADALTFGAVMSLDAFAQAFAGVALVTYMSSLTSLGYTATQYAMLSSTYALLGKLLKG
                     FSGVAVDALTPSHGLLGAYATAFIGTALTAIPPLLLFLLLWRWQRGAASREA"
     gene            1035..1550
                     /locus_tag="VFV25_05905"
     CDS             1035..1550
                     /locus_tag="VFV25_05905"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEY1392887.1"
                     /translation="MTAPPRTARSPSFSATEFRAALGMFATGVTIVTACSPEGTPMGL
                     TANSFNSVSLNPPLVLWSLSRRAGSLPAFSRGSHYAINILAADQRELAERFGGKPNSE
                     RFAGLSWQEGVGGAPLIAGAAAVFECFNRSQYEEGDHVIFVGEVERCSRREGTSPLIF
                     HGGRYFTELPL"
     gene            1625..>2029
                     /locus_tag="VFV25_05910"
     CDS             1625..>2029
                     /locus_tag="VFV25_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEY1392888.1"
                     /translation="MRFRPRIRHCLAAAALACSGVLAAEPDEGPLKLNPFHDPFVSAT
                     SGRACPAPRGPAYSEARIRREGHYRAERGESCFVDGRCAESSAYKYDERIATGVVAAL
                     RADASLADTAIWVISEGRVVSLQGCVGTAEQAA"
BASE COUNT          259 a          735 c          690 g          345 t
ORIGIN      
        1 gccgatggcc tggtggccat cggcgcgttc gcgctgctgg tggccttcgc ctcggccacc
       61 caggacgtcg cgatcgacgc ctggcgcatc gaggccgccg acgatgcgca ggaggtgggg
      121 ctgctgacct ccgccgccca gctcggctac cgtatcgcga tgctggtgac cgacgccgct
      181 atcatcgccg cggcagggcg catcggctgg ccgatgtctt atgtggcgat ggcggtgctc
      241 atgggcgtcg gcatcgccgc gaccttcttc gccttcgagc cgctgcgcgc cgatcaggtg
      301 ctgcacgaca agccgccgct gtggagccgg cgcggcctgg tggacgcgat gatcggcccc
      361 ttcgtggatt tcttcgccaa gcacaaggcc gtggggctgg tgatcctgct ggcggtggcg
      421 ctgtaccggc tgcccgactt catcatgggg ccgatgtaca acccctacta ccacgatctg
      481 ggcatcgcca aggacacggt ggccctggtg cgcggcagct tcggcctggc cgcgaccctg
      541 gtcggcctgg ccgcgggcgg cgtgatgtcg gtgcgcgtgg gcctgctgcc gacgctggtc
      601 gcggggctgg cgctcgaggc cctgggcacc ggctccttcg cgctgctggc gctccatgcc
      661 gatgcgctca ccttcggcgc ggtgatgtcg ctcgatgcct tcgcccaggc cttcgccggc
      721 gtggcgctgg tcacctacat gtccagcctc accagcctgg gctacaccgc gacgcagtac
      781 gcgatgctgt cgtccaccta tgccttgctc ggcaagctgc tcaagggctt ctcgggcgtg
      841 gcggtggacg ccctcacgcc gagccacggg ctgctgggcg cctatgccac ggccttcatc
      901 ggcactgcgc tcacagctat tccgccgctg ctgttgttcc tgctgctgtg gcggtggcag
      961 cggggagcgg cctcgaggga ggcgtgaagt cttgactctg cgccaagccc gtcgcttggc
     1021 acaatcgcga catcatgact gccccgcccc gcaccgcgcg cagccccagc ttcagcgcga
     1081 ccgagttccg cgccgcttta ggcatgttcg ccaccggggt cacgatcgtc acggcgtgca
     1141 gccccgaagg gacgccgatg gggctgacgg ccaactcgtt caactcggtg tcgctgaacc
     1201 cgccgctggt gctgtggagc ctgtctcgcc gcgccggctc gctgcccgcc ttctcgcgcg
     1261 gctcgcatta cgcgatcaac atcctcgccg cggaccagcg cgagctggcc gagcgcttcg
     1321 gcggcaagcc caacagcgag cgcttcgccg gcctgtcctg gcaggaaggg gtgggcggcg
     1381 cgccgctcat cgccggcgcc gccgccgtgt tcgagtgctt caaccgcagc cagtacgaag
     1441 agggcgacca tgtcatcttc gtcggcgagg tggaacgctg ctcgcgccgc gagggaacgt
     1501 cgccgctgat cttccacggc gggcgctact tcaccgagct gccgctttag aaagcagaag
     1561 ccgcacgctg cgcgggcact gagcctgctt gcgatggcgc aaggcctggc cctacactgc
     1621 gtcgatgcga tttcgacctc gcatccgcca ttgcctcgcc gccgccgcat tggcctgctc
     1681 cggcgttctg gccgccgagc cggacgaggg gccgctcaag ctcaacccct tccacgatcc
     1741 tttcgtttcc gccactagcg gccgagcctg cccggcgccg cgcggcccgg cctatagcga
     1801 ggcacgcatc cgccgcgagg ggcactaccg ggccgagcgc ggcgaaagct gtttcgtcga
     1861 cggccgctgc gccgaatcca gcgcctacaa gtacgacgag cgcatcgcca ccggcgtggt
     1921 cgccgccctg cgcgccgatg ccagcctcgc cgacactgcg atctgggtca tctccgaggg
     1981 gcgcgtcgtc tcgctgcagg gctgcgtggg cacggcggaa caggccgcg
//
LOCUS       DASUCY010000304         4077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_986483, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000304 DASUCY010000000
VERSION     DASUCY010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986483"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="VFV25_05915"
     CDS             complement(<1..541)
                     /locus_tag="VFV25_05915"
                     /inference="COORDINATES: protein motif:HMM:NF017727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEY1392889.1"
                     /translation="MRVTPIALATRLPRGYPARWRWRTLFEAPHRLAFAAAVLLLAAS
                     AQWWAALQLAREAGIAVHSALPPSIVHGLVMGLGFMPLFFTGFMFTAGPRWLAVAPVA
                     AARLLPAITAQLAGWGVFMLAAHGRDVAFGETLGALSLAAVACGWFMNWLAFRRLLRV
                     STVADKLHARILLAASLVGL"
     gene            complement(543..1730)
                     /locus_tag="VFV25_05920"
     CDS             complement(543..1730)
                     /locus_tag="VFV25_05920"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HEY1392890.1"
                     /translation="MLRLLERSGLAPLRLAGRTLLPIVQGGMGIGVSAHRLAGSVAAL
                     GAVGTLSSVDLRRHHPDLMERTRGLGFGPEVKEAINAANLEALEREIRGARALAGPCG
                     LLAMNVMRAVGEYAAYVQRSLRAGIDAIVVGAGLPLDLPDMAAEYPGTALIPILSDAR
                     GVQLVVKKWERKKRMPDAIVLEHPAYAGGHLGAARIEDLQDPRFDFERAVPETLAVLR
                     AAGIEASTPVIAAGGIRSLEDIKRVVGLGAAGVQLGTPFAVTCESDAHPEFKRVLAEA
                     KEEDKLEFTSVAGLPARAVATPWLKAYLRAESKLSAVAHVKQRCTKVFDCLAQCGLRD
                     GKAGWGQFCIDQQLASALKGDVKRGLFFTGRGALPFGDQIRSVRELLFKLLGGDAAEA
                     VPA"
     gene            1896..2426
                     /locus_tag="VFV25_05925"
     CDS             1896..2426
                     /locus_tag="VFV25_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEY1392891.1"
                     /translation="MPHRDDPDASPLASIEAGALQDPALLRRLLRAKDRMDAASHEAW
                     PVKRLAEVSGVSEAHFARSFKQAFGIPPHRYLLTRRIEQATTLLRDTELSITEIAFAT
                     GWESLGTFGRTFRDITGQSPSAQRLEARANLPQLDRVPACVLKAAQRPDLTIAVLEKR
                     RRVAKGTNRPSTKEVR"
     gene            2423..2839
                     /locus_tag="VFV25_05930"
     CDS             2423..2839
                     /locus_tag="VFV25_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011489020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY1392892.1"
                     /translation="MNQGINVVGLYVDDQDEALAFYVDKLGFRVHTDVRNGSYRWLTV
                     QHPDQPSFQLGLFKPGPPVHDEATAQSLSAMVAKGAMPPLVLTVADCRASYTQLKARG
                     VEFTQEPVDRFGSVDAGFRDPAGNGWKMIQAPRSTT"
     gene            2836..3099
                     /locus_tag="VFV25_05935"
     CDS             2836..3099
                     /locus_tag="VFV25_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392893.1"
                     /translation="MSNTSAWIRRFHRWVSIAFTLTVIANFVAMAQGNGTPPPWITYA
                     PLLPLALLLFTGLYLFALPYLNRRSTRRSVAVPAVTASESMKV"
     gene            3109..3525
                     /locus_tag="VFV25_05940"
     CDS             3109..3525
                     /locus_tag="VFV25_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEY1392894.1"
                     /translation="MKSSGSTEGRPASELIDKRIADLADWRGETLSRMRQLIKEADPD
                     VVEEWKWMGTPVWSHDGIICTGEHYKSTVKLTFAKGASLKDPAKLFNWSLDGNTRRAI
                     DIREGEEVDAGAFKALVRAAVALNASGAKAGPRRAK"
     gene            complement(3543..3854)
                     /locus_tag="VFV25_05945"
     CDS             complement(3543..3854)
                     /locus_tag="VFV25_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392895.1"
                     /translation="MLRTFLLVVAVALLAIGLVARLAGHAAATPMVIWGAVLLLAVLV
                     ERWRYRPPTRSEGGEWIKTNERFIDPESGRAMHVLYNPRTGERRYGDSSDEQTHYPGD
                     R"
     gene            complement(3863..>4077)
                     /locus_tag="VFV25_05950"
     CDS             complement(3863..>4077)
                     /locus_tag="VFV25_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HEY1392896.1"
                     /translation="LRSLVGGEITEYTQLLEEARRHAIDRMVKNAHQMGGNAVVMMRF
                     DSSEMGQTMSEIVAYGTAVVVEKLPQ"
BASE COUNT          728 a         1424 c         1306 g          619 t
ORIGIN      
        1 ccaggccgac gaggcttgcc gcgagaagaa tccgggcgtg cagcttgtcg gccacggtgc
       61 taacccgcag caggcgacga aaggccagcc agttcatgaa ccagccgcag gccaccgcgg
      121 ccaggctcag tgcacccaac gtctcgccga aggcgacatc tcggccatgg gctgcaagca
      181 tgaatacgcc ccatcctgcc agctgggccg tgatggccgg caacagccgg gccgccgcga
      241 ccggcgccac cgcaagccag cgcgggccgg ccgtgaacat gaagccggtg aagaagagcg
      301 gcatgaagcc caggcccatg accaggccat gcacgatcga aggcggcagg gcactgtgca
      361 ccgcgatgcc cgcctcgcgc gccagctgca aggcggccca ccactgcgcc gaggccgcca
      421 gcagcaagac cgcggcggcg aaggcgagcc ggtgcggtgc ttcgaacagc gtgcgccagc
      481 gccagcgggc ggggtatccc cgcggcagcc gggttgccag cgcgatcggc gttacgcgca
      541 tttcatgcag gcacagcctc cgcggcatcg ccgccgagca gcttgaagag cagttcgcgc
      601 acgctgcgga tctggtcgcc gaagggcagc gcgccgcgcc cggtgaagaa gaggccgcgc
      661 ttcacgtcgc ccttcaaggc cgaggcgagc tgctggtcga tgcagaactg accccagccg
      721 gccttgccgt cgcgcagccc gcattgggcc aggcagtcga agaccttggt gcagcgctgc
      781 ttgacatggg cgaccgcgct cagcttgctc tcggcgcgca gataggcctt gagccagggc
      841 gtggcaacgg cgcgcgccgg caggcccgcg acgctcgtga actcgagctt gtcctcttcc
      901 ttcgcctccg ccagcacgcg cttgaactcg ggatgggcat cgctctcgca ggtgaccgcg
      961 aagggcgtgc cgagctgcac gcccgccgcg cccaggccga ccacgcgctt gatgtcttcc
     1021 agcgagcgga tgccgccggc cgcgatcacc ggcgtgctgg cctcgatgcc ggcagcgcgc
     1081 agcacggcga gcgtctcggg cacggcgcgc tcgaagtcga agcgcggatc ctgcagatcc
     1141 tcgatgcggg ccgcgccgag atgcccgccc gcgtaggccg ggtgctccag cacgatcgca
     1201 tcgggcatgc gcttcttgcg ctcccatttc ttgaccacga gttgcacacc gcgcgcatcg
     1261 gagaggatgg ggatcagggc cgtgccggga tattcggcgg ccatatcagg caaatccagc
     1321 ggcagcccgg cgccgaccac gatcgcgtcg atgccggcgc gcagcgagcg ctgcacatag
     1381 gcggcgtatt cgccaacggc acgcatcacg ttcatcgcca gcagaccgca agggccagcg
     1441 agcgcgcggg cgccgcggat ctcgcgctcc agggcttcga ggttggcggc attgatcgcc
     1501 tccttgacct cggggccgaa gcccaggccg cgcgtgcgtt ccatcaggtc ggggtggtgg
     1561 cgcctcaggt cgacgctgct cagggtgccg accgcgccga gcgccgccac gctgccggcg
     1621 agccggtgcg cggagacgcc gatgcccatg ccgccctgca cgatgggcag cagcgtacgc
     1681 ccggccaggc gcaggggtgc gaggccactg cgctcgagca ggcgcagcat ctcaggtgga
     1741 atcaaggcgg gctccgggag caaagcccgc actctaggca gcggcggtgg ccttgtccct
     1801 gatcggcatc aaatgtttgt caggcgccgc ggcccaagtc tgaacctata gcgttcaagc
     1861 taagctcccc gcatacgcct gcaccctagc aattcatgcc tcatcgcgac gaccctgacg
     1921 cctcgcccct tgcatcgatt gaagccggtg cgcttcaaga tccggcgctg ctgcgccgcc
     1981 tgctgcgtgc gaaggaccgc atggacgccg cctcgcacga ggcctggccc gtcaagcgcc
     2041 tggccgaggt cagcggcgtc tccgaagccc atttcgcgcg ttccttcaag caggccttcg
     2101 gcatcccgcc gcaccgctac ctgctgacgc ggcgcatcga gcaggccacc accctgttgc
     2161 gcgacaccga gctcagcatc acggaaattg ccttcgccac cggctgggag agcctgggca
     2221 ccttcgggcg cacgtttcgc gacatcaccg gccaaagccc cagcgcccag cgcctcgagg
     2281 cacgggccaa cctgcctcaa ctcgaccgcg tgcctgcctg cgtcctgaag gccgcgcagc
     2341 ggcccgacct caccatcgca gttttggaga agcgtcgccg cgtggccaaa ggtacaaacc
     2401 ggccatcaac caaggaggtg cgatgaacca aggtatcaat gtggtgggct tgtatgtgga
     2461 cgaccaggac gaggcgcttg cgttctacgt cgacaagctc ggattccggg tccacacgga
     2521 cgtgcgcaac ggttcctatc gctggctcac ggtacagcat ccggaccagc cctcgttcca
     2581 actcggcctg ttcaaacccg gcccgccggt acacgacgaa gccaccgcgc aatccctgag
     2641 cgccatggtc gcgaagggag ccatgccacc cctggtcctc accgtggccg actgccgcgc
     2701 cagttacacc cagctgaaag cccgcggcgt ggaattcacc caggagccgg tggaccgctt
     2761 cggcagcgtc gatgccggct tccgcgaccc cgccggcaac ggctggaaga tgatccaggc
     2821 cccgaggagc acgacgtgag caacaccagc gcctggattc gtcggtttca ccgctgggtg
     2881 tccattgcct ttacgctgac cgtcatcgcc aacttcgtcg ccatggccca aggaaacggc
     2941 accccgccgc cctggatcac ctacgcaccg ctgctgccgc tggccctctt gctgttcacc
     3001 gggctctacc tgttcgcgct gccctatctc aaccgtcgca gcacgcggcg ttccgtagcc
     3061 gtgcccgccg tgacggcgtc cgaatccatg aaggtctagg gggagaacat gaaatcatcg
     3121 ggttcgaccg agggccggcc ggcatctgag ctgatcgaca aaaggatcgc cgatctcgcg
     3181 gattggcgcg gggagacctt gagcaggatg cgccagctca tcaaggaagc agacccggat
     3241 gtcgtggagg agtggaaatg gatgggcact cccgtctggt cgcacgacgg gatcatctgc
     3301 accggcgagc attacaagtc gaccgttaag ttgacctttg ccaagggagc ttcgttgaag
     3361 gacccagcca agctcttcaa ctggagcctc gacgggaaca cgagacgcgc gatcgacatc
     3421 cgtgaagggg aggaggtcga cgctggcgcg ttcaaggcgc tcgtccgtgc cgcggtcgcc
     3481 ctcaatgcat caggcgcaaa ggcagggccg agacgcgcga agtaggctgg ggcttggccc
     3541 cttcagcgat ctccgggata gtgcgtctgc tcatccgagg agtcgccgta ccgccgctct
     3601 cccgtgcggg gattgtagag cacgtgcata gcgcgccccg actccgggtc gatgaaccgc
     3661 tcgttcgtct ttatccattc gcccccctcg gaacgcgtcg gaggccgata gcgccatcgc
     3721 tcgaccagta ccgccaatag caaaacggca ccccaaataa ccatgggtgt tgctgcagca
     3781 tggcctgcaa ggcgagccac caggcctatt gccaacagcg ccaccgccac caccagcaaa
     3841 aaggtgcgaa gcatggcgct atttattgcg gcaacttctc gaccacgacg gcggtgccgt
     3901 aagccacgat ctcgctcatg gtctggccca tttccgacga gtcgaaccgc atcatgacca
     3961 cggcgttgcc gcccatctga tgcgcgttct tgaccatccg atcgatcgca tggcggcgag
     4021 cctcttccag caattgcgtg tattcggtga tctcgccgcc taccagcgaa cgcagcc
//
LOCUS       DASUCY010000305         3820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_424182, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000305 DASUCY010000000
VERSION     DASUCY010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424182"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VFV25_05955"
     CDS             complement(<1..692)
                     /locus_tag="VFV25_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HEY1392897.1"
                     /translation="MSLRDLSLSAFVAGFVAVLVGFTSSVAIVFQAALAFGATPAQIS
                     SWMWALGLGMGLCTLLPSLWWRKPVMIAWSTPGAAVLATAGAGFTMAEAVGAFLVCAA
                     LITLAGATGWFERVMKRIPLALASALLAGVLARFGIEAFAAAKTALPLVCLMLGTYVV
                     GRRFWPRYAVVVVLAAGVAYAASQGQLRGEALRFDWTLPVFTAPRFSFSAIVSLALPL
                     FIVTMASQNLPGV"
     gene            complement(712..2601)
                     /locus_tag="VFV25_05960"
     CDS             complement(712..2601)
                     /locus_tag="VFV25_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEY1392898.1"
                     /translation="MSPHTPHAPTQSTLAALTLGALGVVYGDIGTSPLYAFKEVFNAG
                     HVPLTLPNILGVLSLVFWTLTIIVSLKYVVLVLRADNNGEGGTMALMALASKVTRGQP
                     RMRHALVIMGLAGAALFYGDGVITPAISVLSAIEGLEVATPTFKPYVVPITLVILFGL
                     YSVQKHGTGLVGKFFGPIMVVWFLVIGVAGGFWVLREPKVLLALNPVWALEFAINNGW
                     ITIVVLGAVFLTVTGAEALYADMGHFGKRPIRLAWFIIVFPSLILCYLGQGSLVLADP
                     KAAANPFFNLVPAWFLYPMVVLSGVATVIASQATISGTFSVTKQAVQLGYLPRMSVLH
                     TSVRETGQIYVPAVNWLQFGAIVTAVALFGSSDALASAYGIAVTGTMVLTTLMTFYVV
                     HYRWNLPWWLSIAATSFFLVIELVFFGANIIKVLHGGWFTLMIAGASFAVLMTWSRGR
                     GLLRERLHEDSIDLRSFLEAVFVSPPARVPGTAVFMTAEAGMTPNALMHNLKHNKVLH
                     QQNIFVNVQQHEVPWVGFDKRMEVESLGHDCWQVKLHFGFKNEPDVPYALELLESVGV
                     KLDPMETSYFLSRENVIPTVVGASMAPWREKLFASMHRNASSAADFLNLPINRVVELG
                     SKVEL"
     gene            complement(2708..>3820)
                     /gene="gshA"
                     /locus_tag="VFV25_05965"
     CDS             complement(2708..>3820)
                     /gene="gshA"
                     /locus_tag="VFV25_05965"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEY1392899.1"
                     /translation="LYPGGFNNLTPEMLPLAVQAAMAAIEKICPEAKNLLLIPEKHTR
                     NTFYLTNVARLMQIFTQAGLNVRLGTLDENVTEPTPLALPDGNSITVEPLMRSKRRLG
                     LKDFDPCTILLNNDLSAGIPKVLEDIHEQYLLPPLHAGWAVRRKSQHFAAYEEVAKKF
                     AKLLGMDPWLINPMFAQCGEVDFTEGGGLECLQSNVDALLGKIRRKYKEYGINEKPFV
                     VVKADNGTYGMGIMTVRDAKDLTELNRRTRNKMSVIKDGQEVSQVIIQEGVPTYERIN
                     DAVAEPVVYMIDRYVVGGFYRVHAERGIDENLNAPGASFVPLAFAESHHLPRPGEKPG
                     ASAPNRFYMYGVVGRLAMLAASYELEATDPDSEIYE"
BASE COUNT          706 a         1237 c         1279 g          598 t
ORIGIN      
        1 acgccgggca ggttctgcga ggccatcgtg acgatgaaga gcggcagcgc caggctcacg
       61 atagccgaga agctgaagcg cggcgccgtg aagaccggca gcgtccaatc gaagcgcagc
      121 gcctcgccgc gcagctggcc ctgcgaggcg gcataggcaa cgcctgcggc cagcaccacg
      181 acgaccgcat agcgcggcca gaagcgccgg ccgacgacat aagtgcccag catcaggcac
      241 accagcggca gcgccgtctt ggccgctgcg aaggcctcga tgccgaagcg cgccagcacg
      301 ccggccagca gcgccgaagc gagcgcgagc gggatgcgct tcatcacgcg ctcgaaccag
      361 ccggtggcac cggccagcgt gatcaacgcc gcgcagacga ggaaagcgcc cacggcctcg
      421 gccatcgtga agccggcccc ggcggtggcc aacacggccg cgcccggcgt tgaccaggcg
      481 atcatcaccg gcttgcgcca ccacagggaa ggcagcaagg tacacagccc catccccagc
      541 cccagggccc acatccagga gctgatctgc gcgggcgtgg cgccgaaggc gagcgcggcc
      601 tggaagacga tcgccaccga actggtgaag cccaccagca cggcgacgaa gccggcgacg
      661 aaggcggaca gcgagagatc gcgcaggctc aagcgccccg gggctgcagc ctcagagctc
      721 aaccttggac cccagctcca ccacgcggtt gatcggcaga ttcaagaagt cggccgccga
      781 cgaggcgttg cggtgcatgc tggcgaacag cttctcgcgc cagggcgcca tgctcgcgcc
      841 gaccacggtg gggatcacgt tctcgcgcga gaggaaatag ctggtctcca tcgggtcgag
      901 cttgaccccc acgctttcca acagctccag cgcatagggc acgtcgggct cgttcttgaa
      961 gccgaaatgc agcttgacct gccagcagtc gtgacccagg gattcgacct ccatgcgctt
     1021 gtcgaagccg acccagggca cctcgtgctg ctgcacgttg acgaagatgt tctgctgatg
     1081 cagcaccttg ttgtgcttga ggttgtgcat cagcgcattg ggcgtcatgc cggcttctgc
     1141 ggtcatgaac acggccgtgc caggcacgcg ggccggcggg ctgacgaaga cggcctccag
     1201 gaagctgcgc aggtcgatcg agtcttcatg caggcgctct cgcagcaggc cgcggccgcg
     1261 gctccaggtc atcagcaccg cgaaagaggc gcctgcgatc atcagcgtga accagccgcc
     1321 atgcagcacc ttgatgatgt tggcgccgaa gaagacgagt tcgatgacga ggaagaagct
     1381 ggtggccgcg atgctcagcc accagggcag gttccaacga tagtggacca cgtagaaagt
     1441 catcaacgtc gtcaacacca tggtgccggt cacggcgatg ccgtaggccg aggccagcgc
     1501 gtcggaggag ccgaacaagg ccaccgcggt gacgatggcg ccgaactgca gccagttgac
     1561 cgcgggtacg tagatctgcc cagtctcgcg caccgaggtg tgcagcaccg acatgcgcgg
     1621 cagatagccg agctgcaccg cctgcttggt caccgagaag gtgcccgaga tggtggcctg
     1681 ggaggcgatc accgtggcca ccccggacag caccaccatg ggatagagga accaggcggg
     1741 caccaggttg aagaaggggt tggctgcggc cttcgggtcg gccagcacca gcgagccctg
     1801 gccgagatag cacaggatca gcgaggggaa gacgatgatg aaccaggcca ggcgtatcgg
     1861 ccgcttgccg aaatgcccca tgtcggcata gagcgcctcg gcgccggtca cggtcaggaa
     1921 caccgcgccc agcacgacga tggtgatcca gccgttgttg atggcgaatt ccagcgccca
     1981 cacgggattg agcgcgagca gtaccttggg ctcgcgcagc acccaaaacc cacccgccac
     2041 gccgatgacg aggaaccaca ccaccatgat gggaccgaag aatttgccca ccaggccggt
     2101 gccgtgcttc tgcaccgaat acaggccgaa caaaatcacc agcgtgatcg gcacgacata
     2161 gggcttgaag gtgggcgtcg cgacctccag gccctcgatg gccgacagca ccgagatcgc
     2221 cggcgtgatc acaccgtcgc cgtagaacag cgccgcgccg gccaggccca tgatcaccag
     2281 cgcatggcgc atgcgcggct ggccgcgcgt gaccttgctg gccagggcca tcagcgccat
     2341 cgtgccgccc tcgccgttgt tgtcggcgcg tagcaccagt accacgtact tcagcgagac
     2401 gatgatggtc agcgtccaga acaccagcga cagcacgccc aggatattgg gtagcgtcag
     2461 cggcacatgg ccggcgttga agacttcctt gaaggcgtac agcgggctgg tgccgatgtc
     2521 gccgtagacg acgcctagtg cgcccagcgt cagggcagcg agcgtggact gggtcggtgc
     2581 gtggggtgta tgcggactca cgagcggcct gcgcagagct caaagactgg cattgtgccc
     2641 cgcacaagcc tgcggccgga agccccgcgg cgcggcaccc ctcaggcgcg ggccggtgag
     2701 cggctgctta ctcgtagatt tcgctgtcgg ggtcggtcgc ttcgagctcg tagctggccg
     2761 ccagcatcgc aaggcgtccg accacgccat acatgtagaa gcggttgggc gcgctggcgc
     2821 ccggcttctc gcccggcctg ggcaggtggt gggattcggc gaaggccagc ggcacgaagc
     2881 ttgcccccgg cgcattgagg ttctcgtcga tgccgcgttc ggcgtgcacg cggtagaagc
     2941 cgcccaccac atagcggtcg atcatgtaga ccaccggctc ggccaccgca tcgttgatgc
     3001 gctcgtaggt cggcacgcct tcctggatga tgacttggct gacttcctgg ccgtccttga
     3061 tcacgctcat cttgttgcgc gtgcggcggt tcagctcggt caggtccttg gcgtcgcgca
     3121 ccgtcatgat gcccatgccg taggtgccgt tgtcggcctt gacgacgacg aagggcttct
     3181 cgttgatgcc atattccttg tacttgcggc ggatcttgcc gagcagcgca tcgacattgc
     3241 tctgcagaca ctccagtccg ccgccctcgg tgaagtcgac ctcgccgcat tgcgcgaaca
     3301 tcgggttgat cagccagggg tccataccca gcagcttggc gaacttcttg gccacctctt
     3361 cataggcggc gaaatgctgg ctcttgcgcc gcaccgccca gccggcgtgc agcggcggca
     3421 gcaggtattg ctcgtggatg tcctccagca ctttgggaat gccggccgat aggtcgttgt
     3481 tgagcaggat ggtgcagggg tcgaagtcct tcagtcccag gcggcgcttg ctgcgcatca
     3541 gcggctcgac cgtgatgctg ttgccgtcgg gcagcgcgag cggggtcggc tcggtgacgt
     3601 tctcgtccag cgtgcccagc ctcacgttga ggccggcctg ggtgaagatc tgcatcagcc
     3661 gcgcgacatt ggtgaggtag aaggtgttgc gcgtgtgctt ctctggaatc agcagcaggt
     3721 tcttggcctc ggggcagatc ttctcgatcg ccgccattgc ggcctgcacc gccagcggca
     3781 gcatctcggg cgtgaggttg ttgaagccgc cggggtagag
//
LOCUS       DASUCY010000306         2196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_102883, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000306 DASUCY010000000
VERSION     DASUCY010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102883"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VFV25_05970"
     CDS             <1..367
                     /locus_tag="VFV25_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392900.1"
                     /translation="YISHVNRKTCGARVRCQRESAAMPQGDRRMQRFTHEITELRLLG
                     FAESLPIHVTDRADDVVARLECGRIGVAVEFRASSSLPQYTIKALVSPAGQGLPLATD
                     VWSAVVQTVHRRAPPNDDE"
     gene            complement(431..1609)
                     /locus_tag="VFV25_05975"
     CDS             complement(431..1609)
                     /locus_tag="VFV25_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HEY1392901.1"
                     /translation="MTTEKTLGFATRVIHAGQAPDPSTGAIMPPIYATSTYVQQSPGV
                     HKGLDYGRSHNPTRWALERCVADLENGAGGFAFASGLAAISTVLELLDSGAHIVASDD
                     LYGGSYRLFERVRKRSAGLSFSFVDMANLDNLVAAIRAETRMVWVETPSNPLLKLCDL
                     AAIAQICRERELIAVADNTFASPWVQRPLELGFDIVVHSTTKYLNGHSDVIGGVAIVG
                     KEPRQAEWLERLGFLQNSVGAIAGPFDSFLTLRGVKTLALRMERHCSSALALAQWLEQ
                     QPKVRRVRYPGLASHPQHELARRQMRNGYGGMISLELDTDLAGARRFLERCELFALAE
                     SLGGVESLIEHPAIMTHASIPAEQRARLGIGDGLVRLSVGVEDANDLRRDLERALGAI
                     "
     gene            complement(1606..>2196)
                     /locus_tag="VFV25_05980"
     CDS             complement(1606..>2196)
                     /locus_tag="VFV25_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY1392902.1"
                     /translation="ARALLKAEGLMGGSSTGTLLAAALRYCREQTAPKRVVSFVVDTG
                     TRYLSKVYNDSWMIDEGLLHRREYGDLRDLIARRQDEGGVVSVEPQDTMLTAFQRMRL
                     ADVSQLPVLDGGKLVGVIDESDVLLKVQSDAAHYAATVQTAMTASPQTLAPNASLAEL
                     RKVLDSGLVAIIADAAGFYGLITRYDLLNHLRKHRS"
BASE COUNT          350 a          708 c          762 g          376 t
ORIGIN      
        1 gtatatttcc cacgtcaacc ggaaaacctg cggagctcgt gtccgctgcc agcgcgagtc
       61 cgctgccatg ccgcaggggg acaggagaat gcagcgcttc acccatgaga tcaccgagct
      121 tcgtctgctc ggctttgcgg agagtcttcc cattcatgtg accgaccgcg cggacgacgt
      181 ggtagctcgg ctcgagtgcg gccgcatcgg ggtcgcggtc gaattccgag ccagttcgag
      241 tctgccgcag tacacgatca aggccttggt ctcgcctgct ggacagggtc tacccttggc
      301 gacagacgtt tggagtgccg ttgtgcaaac cgttcaccgg cgagccccgc cgaacgatga
      361 tgaatgagca cgtgggatac gtgttcaccg cctcgaagtt tgggcgcgcc gcaatagcag
      421 caagctcgct tcaaatcgct cccagcgccc tctccaaatc ccgccgcaga tcgttcgcat
      481 cctccacccc caccgatagc cgcaccagcc cgtcgcctat gcccagcctg gcgcgctgct
      541 cggccgggat gctggcgtgg gtcatgatgg ccgggtgctc gatcaggctc tcgacgccgc
      601 ccaggctctc ggccagcgcg aagagttcgc agcgctccag gaagcggcgc gcgccggcga
      661 ggtcggtgtc gagctcgagc gagatcatgc cgccgtagcc gttgcgcatc tggcgcctgg
      721 ccagttcgtg ctggggatgc gaggcgaggc cggggtagcg cacgcgcctg accttgggct
      781 gctgctcgag ccactgcgcc agcgccagcg cgctgctgca atggcgctcc atgcgcagcg
      841 ccagggtttt cacgccgcgc agcgtgagga agctgtcgaa ggggccggcg atggccccga
      901 ccgagttctg caaaaagccc agacgctcca gccattcggc ctggcgcggt tccttgccga
      961 cgatggcgac gccgccgatg acgtccgagt gcccgttcag atacttggtg gtggagtgca
     1021 ccacgatgtc gaagcccagc tccagcgggc gctgcaccca gggactggca aaggtgttgt
     1081 cggccacggc gatgagctcg cgttcacggc agatctgggc gatggcggcg aggtcgcaca
     1141 gtttgagcag cgggttgctc ggcgtctcga cccagaccat gcgtgtctcg gcgcggatgg
     1201 cggcgacgag gttgtcgaga ttcgccatgt ccacgaagct gaagctcaga cccgcgctgc
     1261 gcttgcgcac gcgctcgaag aggcggtagc tgccgccgta caagtcgtcg cttgcgacga
     1321 tgtgggcgcc gctgtcgagc agctccagca cggtggagat ggcggccagg ccggaggcga
     1381 aggcgaagcc gcccgcgccg ttttccaggt cggccacgca gcgctccagc gcccagcgcg
     1441 tggggttgtg cgagcggcca tagtccaggc ccttgtgcac gccagggctc tgttgcacat
     1501 aggtcgaggt ggcgtagatc ggcggcatga tggcgccggt cgacgggtcg ggtgcctggc
     1561 cggcgtggat cacgcgggtc gcgaagccca aggttttttc agtcgtcatg agcggtgttt
     1621 cctcaggtga ttgagcaggt cgtagcgggt gatgaggcca tagaagccgg ccgcgtccgc
     1681 gatgatggcc accaggccgc tgtccagcac cttgcgcagc tcggccaggc tggcgttggg
     1741 cgccagggtc tgcggcgagg cggtcatcgc ggtctgcaca gtggcggcgt agtgggccgc
     1801 atcggactgc accttgagca gcacatcgga ctcgtcgatc acgccgacca gcttgccgcc
     1861 atccagcacc ggcagctgcg acacatcggc caggcgcatg cgctggaagg cggtgagcat
     1921 ggtgtcctgg ggctcgacgc tgaccacgcc gccttcgtcc tgacggcgcg cgatgaggtc
     1981 gcgcaggtcg ccgtattcgc gacggtgcag caggccttcg tcgatcatcc aactgtcgtt
     2041 gtagaccttg gacagataac gcgtgccggt gtcgaccacg aagctcacga cgcgcttcgg
     2101 cgccgtctgt tcgcggcaat agcgcagcgc cgcggcaagc agggtgccgg tggatgagcc
     2161 acccatcagc ccttcggcct tgagcagcgc gcgggc
//
LOCUS       DASUCY010000307         3588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_568787, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000307 DASUCY010000000
VERSION     DASUCY010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568787"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /locus_tag="VFV25_05985"
     CDS             complement(<1..1049)
                     /locus_tag="VFV25_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY1392903.1"
                     /translation="MPLLRLPSFLRPPGPAAWLTVAAIQLALLLTPVPAHAKNLSLRE
                     ALAIPAATEAFPPSLPATPVSLPDDWALTRPDYEGPVWYRLDFDARSLDAPLLAAYVE
                     RACSNLEVQLNGHLVYSGGRMNEPVSRNCYHSQLVILPTGLLKPSGNRLDIKVTGFPL
                     HRVTARQRAAGLSPVMIGPLPAMQALHERQIFWNLTLAESVGAMLAVVGVLLLALGRL
                     RKLSHLGYFGLLSCLWAALTARLWWRDIPLGNASVELLICTGFAPVAASIVLFLLRYA
                     GYARDRRIDVALWLQCLAAPLALALAGADHLFEAAMLWYALFGLEVLAAIVAFLHRSW
                     QERSRDFWVMAGILCL"
     gene            1126..2115
                     /locus_tag="VFV25_05990"
     CDS             1126..2115
                     /locus_tag="VFV25_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEY1392904.1"
                     /translation="MILRHAFIPLDNNRLAHLCGSLDEHLRAIEAALDVSITRRNESF
                     SVEGAKKPAERAVALMQSLYDRAWRPIRVDEFQLALREAQQADSANANTAVAVARGVD
                     GGDEIVLRTRRADLAGRTPNQQQYLRNILGHDITLAIGPAGTGKTYLAVACAVDALER
                     STVQRIILTRPAVEAGERLGFLPGDLTQKVDPYLRPLYDALYDLMGFDRVTKAFEKGT
                     LEIAPLAFMRGRTLNHAFVILDEAQNTTPEQMKMFLTRIGFGSRAVVTGDVSQIDLPK
                     GAKSGLIDAERVLRRVRGIALSRFTSADVVRHPLVARIVEAYDAARKTTASDD"
     gene            2115..2570
                     /gene="ybeY"
                     /locus_tag="VFV25_05995"
     CDS             2115..2570
                     /gene="ybeY"
                     /locus_tag="VFV25_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEY1392905.1"
                     /translation="MAAAPKPGLSLSLQFADARHREHLPRHKVARWLRAALEAPAEIT
                     VRIVDADEGRSLNREYRRKDYATNVLTFDYAYEPVVVADLILCAPVVAQEAKAEKKPL
                     EAHYAHLLVHGALHAQGYDHEVEDEAAVMEARESELMLALGFKDPYSQR"
     gene            complement(2571..2963)
                     /locus_tag="VFV25_06000"
     CDS             complement(2571..2963)
                     /locus_tag="VFV25_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HEY1392906.1"
                     /translation="MNADEQAIRDIVATWHRATAAGDVDAVVPLMTGDVVFLTPGNPP
                     MQGRAKFEASLRSLLLTHRVESSWNIEEIQTSGDMAYCWSLLEVRITPLVGGDTVLRT
                     GSALSILRKQASGRWQIVRDANLLSLAK"
     gene            complement(2990..>3588)
                     /locus_tag="VFV25_06005"
     CDS             complement(2990..>3588)
                     /locus_tag="VFV25_06005"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY1392907.1"
                     /translation="TGYYLDQRDNRRLFADVVKHYGCKTALNCYSYTGGFSVAALAGG
                     ASQVISVESSAPALARASAHVALNGYDTTRHETLDADVNATLRRLLKEERSFDAIVLD
                     PPKFAPSAAHAERASRAYKDINRLAFKLLAPGGVLLTFSCSGGIGAELFHKIVAGAGM
                     DAGVDAYLVRRLEAAPDHPTTVNFPEGEYLKGLMLIRR"
BASE COUNT          618 a         1205 c         1260 g          505 t
ORIGIN      
        1 aggcagagga tcccggccat cacccagaag tcgcggctgc gctcttgcca gctgcggtgc
       61 aggaaggcca cgatggccgc cagcacttcc aggccgaaga gcgcgtacca gagcatggca
      121 gcctcgaaga ggtggtccgc accggccagc gccagggcca gcggcgcggc caggcattgc
      181 agccagaggg cgacgtcgat gcggcggtcg cgcgcatagc cggcgtagcg cagcaggaac
      241 agcacgatgc tggcggccac aggcgcgaaa cctgtgcaga tcagcagctc gacgctggca
      301 ttgcccaagg ggatgtcgcg ccaccagagc cgggcggtga gcgccgccca caggcaggac
      361 agcaggccga aatagcccag atggctgagc ttgcgcagac ggcccagcgc cagcagcagc
      421 acgccgacca ccgcaagcat cgccccgacg ctttcggcca gcgtcaggtt ccagaagatc
      481 tgcctctcgt gcagggcctg catggccggc agcgggccga tcatcacggg cgacaggccg
      541 gcagcgcgct ggcgcgccgt gacgcgatgg agcgggaagc ccgtcacctt gatgtcgagc
      601 cggttgcccg acggcttcag caggccggta ggcaggatga cgagctggga gtggtagcaa
      661 ttgcggctca ccggctcgtt catgcgaccg ccgctgtaga cgagatgccc attgagctgc
      721 acctcgaggt tggagcaggc gcgctccaca taggcggcca gcaggggcgc gtcgagcgac
      781 cgcgcgtcga aatccaggcg ataccagacc gggccttcgt aatcggggcg ggtcagcgcc
      841 caatcgtcgg gcagcgagac cggcgtggcc ggcagactcg gaggaaaggc ttcggtggcg
      901 gcgggaatgg ccaaggcctc gcgcaggctc aggttcttgg cgtgggccgg gactggcgtc
      961 agcagcagcg cgagctggat ggcggcgacg gtcaaccacg ccgccggccc tgggggccgg
     1021 agaaacgaag gcaggcgcag cagcggcatg atggctcgcg attgtgcagg atgcccggct
     1081 cccgcgggga ggcgacaatg ctgccccaag actaagccca cttccgtgat cctgcgccac
     1141 gccttcattc ccctggacaa caaccgcctg gcccatctgt gcggctcgct ggacgagcat
     1201 ctgcgcgcca tcgaggcggc gctggatgtg agcatcacgc ggcgcaacga gtctttcagc
     1261 gtcgaagggg cgaagaagcc cgccgagcgg gccgtggccc tgatgcaaag cctctacgac
     1321 cgggcgtggc ggccgatacg cgtcgacgag ttccagctcg cgctgaggga agcccagcag
     1381 gccgatagcg cgaatgcgaa tacggcggta gcggttgcgc gcggtgtgga cggcggcgac
     1441 gagatcgtgc tgcgcacgcg gcgcgccgac ctggccgggc gcacgcccaa ccagcagcaa
     1501 tacctgcgca acatcctggg ccacgacatc acgctggcga tcggcccggc cggcaccggc
     1561 aagacctatc tggcggtggc ctgcgcggtg gatgcgctgg agcgcagcac ggtgcagcgc
     1621 atcatcctca cgcgccccgc ggtcgaggcc ggcgagcggc tgggtttttt gcccggcgac
     1681 ttgacgcaga aggtcgaccc ctacctgcgc ccgctctacg acgcgctcta cgacctgatg
     1741 ggcttcgacc gcgtcaccaa ggccttcgag aagggcacgc tggagatcgc gccgctggcc
     1801 ttcatgcgcg ggcgcacgct gaaccatgcc ttcgtgatcc tcgacgaggc gcagaacacc
     1861 acgcccgagc agatgaagat gttcctcacg cgcatcggct tcggctcgcg cgccgtggtg
     1921 accggcgacg tgagccagat cgacctgccc aagggcgcga agagcggcct gatcgatgcc
     1981 gagcgcgtgc tgcgccgcgt gcgcggcatc gcgctcagcc gcttcacctc ggccgacgtg
     2041 gtgcgccatc cgctggtggc acgcatcgtc gaggcctatg acgcggccag gaagacgacg
     2101 gcgagcgacg actgatggcc gctgctccca aacccggcct gagcctgtcg ctgcaattcg
     2161 ccgacgcgcg ccaccgcgag catctgccgc gccacaaggt ggcgcgctgg ctacgagccg
     2221 cgttggaagc cccggccgag atcacggtgc gcatcgtcga tgccgacgag ggccgcagcc
     2281 tgaaccgcga gtaccgccgc aaggactacg ccaccaatgt gctgaccttc gactatgcgt
     2341 acgagccagt agtcgtggcc gacctgatcc tgtgcgcgcc ggtggtcgcc caggaggcca
     2401 aggccgagaa gaagccgctg gaagcccact acgcccacct gctggtgcac ggcgcgctgc
     2461 atgcgcaggg ctacgaccac gaggtcgagg acgaggcggc ggtgatggaa gcgcgcgaga
     2521 gcgagctgat gctggcgctg ggcttcaagg acccttacag ccagcgctga tcacttcgcc
     2581 agcgacagca gattcgcatc ccgcacgatc tgccagcggc cggacgcctg cttgcgcagg
     2641 atggacagcg cgctgcctgt gcgcaggacc gtgtcgccgc cgacgagcgg cgtgatgcgc
     2701 acctccagca ggctccagca ataggccatg tcgccggacg tctggatctc ctcgatgttc
     2761 cagctcgact cgacgcggtg ggtcagcaac agcgagcgca ggctggcctc gaacttggcc
     2821 cgcccttgca tcggcggatt gccgggcgtc aggaacacga cgtccccagt catcagcggc
     2881 accacggcgt cgacatcgcc ggcggcggtg gcgcggtgcc aggtcgccac gatgtctcgg
     2941 atggcttgct cgtcggcgtt catcgcggct ccttgcatcg agatccggct cagcgtcgga
     3001 tcagcatcag gcccttcagg tactcgccct cgggaaagtt caccgtggtc gggtgatccg
     3061 gcgccgcctc gagacgccgc accaggtagg catccacgcc cgcatccatc cccgcgccgg
     3121 ccacgatctt gtggaagagc tcggcgccga tgccgcccga gcacgagaag gtcagcagca
     3181 cgccgcccgg tgccagcagc ttgaaggcca ggcggttgat gtccttatag gcacgcgagg
     3241 cacgctcggc atgggccgcg ctgggtgcga acttgggcgg gtcgagcacg atggcgtcga
     3301 agctgcgctc ttctttcagc aaacggcgca gcgtcgcgtt cacgtcggca tccagcgtct
     3361 cgtggcgcgt cgtgtcgtag ccgttgagtg ccacatgagc cgaggcgcgg gccagcgcag
     3421 gcgccgagga ctcgacgctg atgacttggc tcgcgccgcc ggccagcgcc gccaccgaga
     3481 agccgccggt gtagctgtag cagttgagcg cggtcttgca gccgtagtgc ttcaccacgt
     3541 cggcgaacag ccggcggttg tcgcgctggt cgaggtagta gccggtct
//
LOCUS       DASUCY010000308         3506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1179146, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000308 DASUCY010000000
VERSION     DASUCY010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179146"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VFV25_06010"
     CDS             complement(<1..413)
                     /locus_tag="VFV25_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEY1392908.1"
                     /translation="MAADGVGINLTNQFLIAMPGMVDDNFVGSVVYMCEHTEKGALGL
                     VINKPIDIKLRRLFEKVELTLEREDLADAPVYFGGPVQTERGFVLHERLGEEGGHYSS
                     TLSIPGGLEMTTSKDVLEALSHGAGPRKVLVTLGYA"
     gene            complement(501..>3506)
                     /locus_tag="VFV25_06015"
     CDS             complement(501..>3506)
                     /locus_tag="VFV25_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY1392909.1"
                     /translation="LAHSLAGSSATVGFADLSQLARQLEHGLMRSQALGRGTPEEAML
                     FVEAAEEVRKLLHQFAAGFLKSPPPELLQRLAEHEVEAAHRLDVQSAAADFAESAEVV
                     AAAAPVFVPPSRPAPLQPAHAVEDHATSSGHGELGQAELKAFVPLEVSSAVAPATPSR
                     SRGGEADFDDEIDAVDVVDPDLFPFFEEEGQELLPLLASQMRDWQRHPSDAAGSGACM
                     RTLHTLKGGARLAGAMRLGEMAHRLETAIEHLLAKAEPSAADVEALQGRVDGLQAVFE
                     ALCARDAQAYAAASAAAVEAAQSLPMPLAELPELPEPFQPTPHVVAQPVVAQPGIAQP
                     ARELAEPAKALEAPAVGAETPATEIDWSRFVSGVPAAREAAAPGERSLASTTHAVRVR
                     AQLLDRMVNQAGEVSITRARLESEVSQIKGSLSDLSDNLERLRQQLRDIEFQAETQMS
                     SRLEAAKQAAQQFDPLEFDRFTRFQEITRMMAESVNDVATVQRSMQRTLETTEDELAA
                     QARLTRDLQDDLLRTRMVEFESLSDRLYRVVRLAAKETGKQVRLDIVGGSIEVDRGVL
                     ERMTGSFEHLLRNCVSHGIEAPAVRQAAGKDASGTIVVVLHQEGNEVSVEFRDDGAGL
                     NLARIRDKGRALGLIAADAQPGDAELANLIFMPGFSTADRVTELAGRGIGMDVVRSEI
                     NAMGGRIETATAAGKGTSFKLVLPLTTAVTQVVMLRCGDATIAVPSNLVEIVRRATPI
                     EVDSAYTRSGYIYGDHALPFFWLGALLQLSPRGLETGRSMPVVVIRSAQQRIALHVDE
                     VLGNQEVVVKNLGPQLSRMPGLAGMTLLASGAVALIYNPVALATVYGETAHAYTAAAL
                     HAPAEQLAAEPEVMINAVPATPLVLVVDDSLTVRRVTQRLLAREGYRVTLAKDGLEAL
                     ERLAEERPAVVLSDIEMPRMDGFDLVRNIRADRALAGLPVIMITSRIAQKHRDYAAEL
                     GVNHYLGKPYSEEELLALIGRYVAEAAVA"
BASE COUNT          542 a         1188 c         1223 g          553 t
ORIGIN      
        1 gcatagccca gggtcaccag caccttcctg gggccggcgc catgggaaag ggcctccagc
       61 acgtccttgc tggtcgtcat ctccaggccg ccggggatgc tgagcgtgga gctgtaatga
      121 ccgccctcct cgcccaggcg ctcgtgcaac acgaagccgc gctcggtctg caccgggccg
      181 ccgaaataga cgggcgcatc ggccaagtcc tcgcgctcaa gcgtgagctc gactttctcg
      241 aacaggcggc gcagcttgat gtcgatcggc ttgttgatga ccaggcccag cgcgcctttt
      301 tcggtgtgct cgcacatgta gacgaccgag ccgacaaagt tgtcgtccac catgccgggc
      361 atggcgatca gaaactggtt ggtaaggttg atgccgacgc cgtccgcagc catgcctgaa
      421 ttctagcggc gccgcacccg gcgcgatcat gaaggcggcc ccccgtttcg ggcaggccgc
      481 gcggcccgag ccgggtgggc tcaggcgacg gccgcctcgg cgacgtagcg gccgatcagc
      541 gccagcagtt cctcttcgct gtagggcttg cccaagtagt gattgacgcc cagctcggcg
      601 gcatagtcgc ggtgcttctg cgcaatgcgc gaggtgatca tgatgaccgg caggccggcc
      661 aaggcccggt cggcgcggat gttgcgcacc aggtcgaagc catccatgcg cggcatctcg
      721 atgtcggaca ggaccacggc cgggcgctcc tcggccaggc gctccagcgc ctccagaccg
      781 tccttggcga gcgtcacgcg atagccctcg cgggccagca ggcgctgcgt gacgcggcgc
      841 acggtgagcg aatcgtccac caccagcacc agcggcgtgg ccggcaccgc attaatcatc
      901 acctcgggct cagcggccag ctgctcggcc ggcgcatgca gcgccgccgc ggtgtaggca
      961 tgggccgttt cgccgtagac cgttgccagc gccacggggt tatagatcag cgcaacggca
     1021 ccggaggcca gcagggtcat gccggcgagg cccggcatgc gcgagagctg cggccccagg
     1081 ttcttcacca ccacttcctg gttgcccagc acttcgtcca cgtgcagggc gatgcgctgc
     1141 tgggccgagc ggatcacgac aacgggcatg gaacgtcccg tctccaggcc gcgcggactc
     1201 aactgcagca gggcgcccag ccagaagaag ggcaaggcat ggtcgccgta gatgtaaccg
     1261 ctgcgcgtgt aggcactgtc gacctcgata ggtgtagcgc ggcgaacgat ctccaccagg
     1321 ttggagggca cggcgatggt cgcatcgccg cagcgcagca tcaccacctg cgtgaccgcc
     1381 gtggtcagcg gcagcacgag cttgaagctg gtgcccttgc ccgcggcggt cgcggtttcg
     1441 atgcggccgc ccatggcgtt gatctccgag cgcacgacgt ccatgccgat gccgcgcccg
     1501 gccagttcgg tgaccctgtc ggccgtggag aagccgggca tgaagatcag attggcgagc
     1561 tcggcgtcgc cgggctgggc gtcggccgcg atcagcccga gcgcgcggcc tttgtcgcga
     1621 atgcgggcga ggttcaggcc ggcaccgtcg tcgcggaact cgacgctgac ttcgttgccc
     1681 tcctgatgca gcacgacgac gatggtgccg ctggcatcct tgccggcggc ttggcgcacg
     1741 gcgggcgcct cgatgccatg gctcacgcag ttgcgcagca gatgctcgaa ggagccggtc
     1801 atgcgctcca gcacgccgcg gtcgacttcg atcgagccgc cgacgatgtc cagacgcacc
     1861 tgcttgccgg tttccttggc ggccagccgc accacgcgat agaggcggtc ggacagactc
     1921 tcgaactcca ccatgcgggt gcgcagcagg tcgtcctgca agtcgcgcgt gagccgcgcc
     1981 tgggccgcca gctcgtcttc ggtggtctcc agcgtgcgct gcatgctgcg ctgcacggtg
     2041 gccacgtcgt tgaccgactc ggccatcatg cgcgtgattt cctggaagcg cgtgaaacgg
     2101 tcgaactcca gcgggtcgaa ctgctgggcc gcctgcttgg cggcctccag gcgcgagctc
     2161 atctgcgtct cggcctgaaa ctcgatgtcg cgcagttgct ggcgcagacg ctccaggttg
     2221 tcgctcaggt cgctcagcga acccttgatc tggctcacct cggactccag ccgcgcgcgc
     2281 gtgatgctga cctcgccggc ctggttgacc atgcgatcga gcagctgtgc acgcacgcgc
     2341 acggcgtggg tggtcgaagc aagggaacgt tcgcccggcg ctgcagcctc gcgcgctgcc
     2401 ggcacgccgg agacaaagcg cgaccagtcg atctcggtcg cgggcgtctc ggccccaaca
     2461 gccggcgctt cgagcgcctt ggccggttcg gcaagctcgc gcgcaggctg agcgatgcca
     2521 ggctgcgcga cgacgggctg cgcgacgaca tgcggtgtcg gctggaaggg ctcaggaagt
     2581 tcgggtagtt cggccagcgg catcggcagc gactgggccg cctccaccgc tgccgccgat
     2641 gcggcggcat aggcctgggc gtcgcgggcg cacagggcct cgaagacggc ctgcaagcca
     2701 tcgacgcggc cttgcagggc ttcgacgtcc gccgcgctgg gctcggcctt ggcgagcagg
     2761 tgctcgatgg ccgtttctag ccggtgcgcc atttcgccca gacgcatcgc gccggccagg
     2821 cgggcaccac ccttgagcgt gtgcagcgtg cgcatgcagg cgccggaacc ggcggcgtcg
     2881 ctgggatggc gctgccagtc gcgcatctgc gaagccagca ggggcaggag ctcctggccc
     2941 tcttcctcga agaaggggaa gagatccggg tccaccacgt cgaccgcatc gatctcgtcg
     3001 tcgaaatcgg cctcgccacc gcgcgagcgg gagggcgttg ccggcgcgac ggcgctggag
     3061 acttcgagcg gcacgaaggc cttcagctcg gcctggccga gttcgccatg accggaggat
     3121 gtggcgtgat cctctactgc gtgcgctggc tgcaatggcg caggcctgct gggcgggacg
     3181 aaaacgggcg cagcggcggc gaccacctcg gccgactcgg cgaagtctgc cgcggcgctt
     3241 tgcacatcga gccgatgtgc cgcttcgacc tcatgctcgg ccaggcgctg gagcaactcg
     3301 ggcggcggcg acttcaggaa gccggcggcg aattgatgca gcagcttgcg cacctcttcc
     3361 gccgcctcga caaagagcat ggcttcttcc ggggtgccgc ggccgagggc ttgcgagcgc
     3421 atcagcccat gctcgagctg ccgcgccagt tgcgacagat cggcaaaacc gactgtggcc
     3481 gagctgccgg ccagcgaatg ggccag
//
LOCUS       DASUCY010000309         2237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_38709, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000309 DASUCY010000000
VERSION     DASUCY010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38709"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="VFV25_06020"
     CDS             complement(<1..583)
                     /locus_tag="VFV25_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEY1392910.1"
                     /translation="MKKWTRDEMAARVAAEIEDGMVVNLGIGLPTLVANHLPREREVI
                     LHSENGVLGMGPAPAKGEEDYDLINAGKQPVTLLPGGAYFHHADSFAMMRGGHLDFCV
                     LGAFQVSATGDLANWHTGAPDAIPAVGGAMDLAIGAKHVYVMMEHVTKSGEAKIVPRC
                     TYPLTGIACVNRIFTDLAVIDVTPRGLKVVEKVE"
     gene            complement(592..1287)
                     /locus_tag="VFV25_06025"
     CDS             complement(592..1287)
                     /locus_tag="VFV25_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HEY1392911.1"
                     /translation="MINKILPNAAAALADVPDGATIMIGGFGTAGQPNELIDALLAQG
                     AKDLVIVNNNAGNGDTGLAALLAAKRVRKIICSFPRQADSHHFDALYRAGQIELELVP
                     QGNLAERIRAAGAGVGAFFTPTGYGTELARAKETREINGRHYVLEYPIHADYALIKAE
                     RGDRWGNLSYRMTARNFGPIMATAAKVAVATVHELVELGAMDPEAVVTPGIYVKRLVK
                     IERTATSAGGFKQ"
     gene            1425..2234
                     /locus_tag="VFV25_06030"
     CDS             1425..2234
                     /locus_tag="VFV25_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY1392912.1"
                     /translation="MSNAASDQLDTAEAPRPGDSYVQSFARGLEVLRSFGADAPAQTL
                     SEAAERAGLTRAGARRILLTLQHLGYVEAEGRLFRLTPKVLELGFAYLSSQPVWQLAQ
                     PVMEELTASLHESCSAAVLDGEDIVYVLRIPASKIMSINLGIGSRLPAYCTSMGRVLL
                     AGLGDEELKHKLAQMKLAAHTPRTITDPERLFEVLQQVRLQGFALVAEELEGGLVSIA
                     VPIVNRAGRAIAAINISGQQHRTPPAHMLEVFLPRLLAAAARINAMVKAQD"
BASE COUNT          348 a          741 c          735 g          413 t
ORIGIN      
        1 cctcgacctt ttcgacgacc ttcagtcccc gcggggtcac gtcgatcacg gcaaggtccg
       61 tgaagatgcg gttcacgcag gcgatgcccg tgagcgggta agtgcagcgc ggcacgatct
      121 tggcctcgcc gctcttggtg acgtgctcca tcatgacgta gacgtgcttc gcaccgatgg
      181 ccaggtccat ggcgccgccc acggccggga tcgcgtcggg cgcgcccgta tgccagttgg
      241 cgaggtctcc ggtcgcggag acctggaagg cgccgagcac gcagaaatcg aggtggccgc
      301 cgcgcatcat cgcgaagctg tcggcgtggt ggaagtatgc gccgcccggc agcagcgtca
      361 ccggctgctt gccggcgttg atgaggtcgt agtcctcctc gcccttggcc ggcgccgggc
      421 ccatgcccag cacgccgttc tcgctgtgca ggatcacctc gcgctcgcgc ggcaggtggt
      481 tggccacgag cgtcggcagg ccgatgccga ggttcaccac catgccatct tcgatctcgg
      541 cggcgacgcg cgcggccatt tcgtcacggg tccatttctt catgtctagc cctattgctt
      601 gaagccgccc gcgctggtgg cggtgcgctc tatcttcacg agacgcttca cgtagatgcc
      661 cggcgtgacc accgcctcgg gatccatggc cccgagctcg accagctcat gcaccgtggc
      721 caccgcgacc ttggctgccg tggccatgat gggcccgaag ttgcgcgccg tcatgcggta
      781 gctgaggttg ccccagcggt cgccgcgctc ggccttgatg agcgcgtagt cggcatggat
      841 ggggtactcc agcacgtagt gccggccgtt gatctcgcgc gtttccttgg ccctcgccag
      901 ctcggtgccg tagcccgtgg gcgtgaagaa ggcgcccacg ccggcaccgg ccgcgcggat
      961 gcgctcggct aggttgcctt gcggcaccag ctccagctcg atctgcccgg cgcggtacag
     1021 cgcgtcgaag tggtgcgaat cggcctggcg cggaaagctg cagatgatct tgcgcacccg
     1081 cttggctgcc agcagcgcgg ccagcccggt atcgccgttg cccgcgttgt tgttgacgat
     1141 gacgagatcc ttggcgccct gggccagcag ggcatcgatc agctcgttgg gctggccggc
     1201 cgtgccgaag ccgccgatca tgatcgtggc gccgtcgggc acgtcggcca gtgctgcggc
     1261 ggcgttgggg aggattttgt tgatcatgtt ctatatatga acaaaacttg aaaaacaccc
     1321 atagatgttc ttataatgaa cttcgattct gtaatcgaac gaatcgagga taggcggtgc
     1381 cttgccggct gtcaaaccgg tggcccttgc ccacttacga agccatgtcc aacgctgcct
     1441 cagaccagct cgatacggcc gaagctccgc gccccggaga cagctatgtc cagtccttcg
     1501 cccgcgggct ggaagtgctg cgcagcttcg gcgccgacgc gccggcccag accctgtccg
     1561 aagcggccga gcgcgccggt ctcacgcgcg ccggtgcgcg ccgcatcctg ctgaccctgc
     1621 agcatctggg ctatgtcgag gccgaggggc ggctgttcag gctcactccc aaggtactgg
     1681 agctgggttt cgcctatctg tcctcgcagc cggtgtggca gctcgcccag ccggtgatgg
     1741 aagaactcac cgcgtcgctg cacgagtcct gctcggctgc ggtgctggac ggcgaggaca
     1801 tcgtctatgt gctgcgcatc ccggccagca agatcatgtc catcaacctc ggcattggca
     1861 gccgattgcc ggcgtactgc acctcgatgg gtcgcgtgct gctggccggc ctgggcgacg
     1921 aggagctcaa gcacaagctc gcgcagatga agctcgccgc acacacgccc aggaccatca
     1981 ccgatcccga gcgcttgttc gaggtactgc agcaggtaag gctgcagggc tttgcactgg
     2041 tggccgagga gctcgaagga gggctggtgt ccatcgccgt gccgattgtc aaccgtgccg
     2101 gccgcgcgat tgccgccatc aacattagcg gccaacagca ccgcacgccg ccggctcaca
     2161 tgctcgaagt cttcctgccc aggcttctgg cagcggcggc ccgcatcaat gcgatggtga
     2221 aggcgcagga ttgatcc
//
LOCUS       DASUCY010000310         2314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_777680, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000310 DASUCY010000000
VERSION     DASUCY010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777680"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="VFV25_06035"
     CDS             <1..816
                     /locus_tag="VFV25_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEY1392913.1"
                     /translation="YVTLGDLHQPSVVLAVIGFFTIVVLDRLKLRGAILIGILLVTVL
                     SFFFAGNKFAGIFDAPPSIAPTFLQLDIKGALGVGVFNVILVFFLVELFDATGTLMAV
                     AKRAGLLAEGKMDRMNKALLADSGAIFAGSLLGTSSTTAYIESASGVQAGGRTGLTAV
                     AVAALFLACLFIAPLAGAVPAYATAPALLYVACLMLRELTELNWDETTDVIPATVTAL
                     AMPLTYSIANGLAFGFITYAVLKLFTGKWREAHAMVWIIGGIFLFKFIYMGGH"
     gene            912..>2314
                     /locus_tag="VFV25_06040"
     CDS             912..>2314
                     /locus_tag="VFV25_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY1392914.1"
                     /translation="MPLRLELVGISKQYPAVKANDGVSLRVAPGQIHAVLGENGAGKS
                     TLMKIIYGAVQPDEGEIRWNGQLVAVRNPHEARALGISMVFQHFSLFDTLTAAENVWL
                     GLSKSISLAEVSERVRKVSHEYGLDVDPLRPVHTLSVGERQRVEIVRALLTKPQLLIL
                     DEPTSVLTPQAVDKLFVTLRQLSREGCSILYISHKLDEIRALCHHCTVLRGGKVTGEV
                     DPAQESNASLSRLMIGAEPPQLQHIQASLGEVALELRGLSLPKLSPFGTTLEDVKLQV
                     RAGEIVGIAGVSGNGQQELMAALSGEDTRAAPGSITLFGQDIARHAPRRRRRAGLHFV
                     PEERLGRGAVPILSLAQNTLLTRTEAVSRRSGWIKVGEVERLAEALIRRFNVKAGGPG
                     AAAKSLSGGNLQKFLVGREIDAAPKLLIVSQPTWGVDVGAAAQIRGELLKLRDAGCAL
                     LVVSEELDELFEISDRLV"
BASE COUNT          354 a          789 c          775 g          396 t
ORIGIN      
        1 tacgtgacgc tgggcgatct gcaccagccc tcggtggtgc tggcggtgat cggcttcttc
       61 acgatcgtcg tgctcgaccg cctgaagctg cgcggcgcca tcctgatcgg catcctgctg
      121 gtgacggtgc tgtccttctt cttcgccggc aacaagttcg ccggcatctt cgatgcgccg
      181 ccctcgatcg cgccgacctt cctgcagctc gacatcaagg gcgcgctggg ggtgggcgtg
      241 ttcaacgtca tcctggtctt cttcctggtc gagctgttcg atgcgaccgg caccttgatg
      301 gcggtcgcca agcgcgccgg cctgctcgcc gaaggcaaga tggaccgcat gaacaaggcc
      361 ctgctggccg acagcggcgc catcttcgcc ggctcgctgc tcggcacctc cagcaccact
      421 gcctatatcg aaagcgcgtc cggcgtgcag gcgggcgggc gcaccggctt gactgcggtg
      481 gctgttgcgg cgctcttcct ggcctgcctc ttcatcgccc cgttggccgg cgcggtgccg
      541 gcctacgcga cggctccggc cctgctctat gtggcttgcc tgatgctgcg cgagttgacc
      601 gaactgaact gggacgagac caccgatgtc atccccgcca ccgtgaccgc gctggccatg
      661 ccgctgacct attcgatcgc caacggcctg gccttcggct tcatcaccta tgcggtgctg
      721 aagctcttca ccggcaagtg gcgcgaggcg catgcgatgg tgtggatcat cggcggcatc
      781 ttcctgttca agttcatcta catgggcggg cactgaacag cgcgctggct ggctcgatca
      841 ttgcttaggc acaggggcac aatccgacca tgcctgccga agctcccact cctccaagta
      901 tggagaactc catgcctttg cgcctcgagc tcgtgggcat cagcaagcag taccccgccg
      961 tcaaggccaa tgacggcgtc agcctgcgcg tggcgcccgg ccagattcac gccgtactcg
     1021 gcgagaacgg cgccggcaag tccacgctga tgaagatcat ctacggagcg gtgcagcccg
     1081 acgagggcga gatccgctgg aacggccagc tcgtggccgt gcgcaacccg cacgaggcca
     1141 gggcgctggg catctcgatg gtgttccagc atttctcgct gttcgacacg ctcaccgcgg
     1201 ccgagaacgt ctggctgggc ttaagcaagt ccatctcgct ggccgaggtg agcgagcgcg
     1261 tacgcaaggt gtcgcatgaa tacgggctcg acgtggaccc gctgcggccg gtgcatacgc
     1321 tgtcggtggg cgagcgccag cgcgtggaga tcgtgcgcgc gctgctcacc aagccgcagc
     1381 tgctcatcct cgacgagccc acgtcggtgc tgacgccgca ggcggtggac aagctcttcg
     1441 tcacgctgcg gcagcttagc cgcgagggct gctccatcct ctacatcagc cacaagctgg
     1501 acgagatccg cgcgctttgc catcactgca ccgtgttgcg cggcggcaag gtgacgggcg
     1561 aggtagaccc cgcccaggaa agcaacgcca gcctgtcgcg cctgatgata ggcgccgagc
     1621 cgccgcagct gcagcacatc caggcctcgc tgggcgaggt ggcgctggag ctgcgcggcc
     1681 tgtccctacc caagctctcg cccttcggta ccacgctcga ggacgtgaag cttcaggtgc
     1741 gcgccggcga gatcgtcggc atcgccgggg tctcgggcaa cggccagcag gagctgatgg
     1801 cggctctctc gggcgaagac acgcgcgccg ccccgggcag catcacgctc ttcggccagg
     1861 acatcgcccg ccacgcgccg cgccggcgcc gcagggcggg cctgcacttc gtgcccgaag
     1921 agcgcctggg acgcggcgcc gtgccgatcc tgtcactggc gcagaacacc ttgctgaccc
     1981 gcaccgaggc ggtgtcgcgc cgcagcggct ggatcaaggt cggcgaagta gagcgcctgg
     2041 ccgaggcgct gatccggcgc ttcaacgtca aggccggcgg gcccggcgct gcggccaaga
     2101 gcctgtcggg cggcaatctg cagaagttcc tggtcgggcg cgagatcgac gcggcgccga
     2161 agctgctcat cgtctcgcag ccgacctggg gcgtggacgt gggcgccgcc gcccagatcc
     2221 gcggcgagtt gctcaagctg cgcgatgccg gctgcgcgct gctcgttgtg agcgaagagc
     2281 tggatgagct gttcgagatc agcgaccggc tggt
//
LOCUS       DASUCY010000311         3312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1564734, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000311 DASUCY010000000
VERSION     DASUCY010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1564734"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /locus_tag="VFV25_06045"
     CDS             complement(<1..1040)
                     /locus_tag="VFV25_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY1392915.1"
                     /translation="MGLALCGLAATAPAAPTAAQPIRLALIEGLSGPFGNAGEAVWRN
                     LAWAVERVNARGGVQLPGGARPLQLLRYDSKGDVGEALSLLKAATDDGAAVVLQGNSS
                     AVAAALIDAVNKRNEREPDRRVLFFNYAAVDPALTNENCSFWHFRFDANADMRLAALS
                     DALRDDAAVKKVYLIGQDYSFGRYVLKQGRALIAAKRPDVEVVGEELHPIGRIKDFLP
                     YAAKIKASGADAVLTGNWGNDLTLLIKAAREAGVDAKFYTFYGNALGAPAAIGEAGVG
                     RVLAVAEWHPNVGTPGSDRFYQSFRQRFPRPEDDYVHARMQAMIELLAQATEKAHSTD
                     AVAVARALEGLRL"
     gene            1172..1618
                     /locus_tag="VFV25_06050"
     CDS             1172..1618
                     /locus_tag="VFV25_06050"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEY1392916.1"
                     /translation="MFLIPSGRHPARNGHAVYFNRAFDRLFDDAFDRAFGEAAPSRAP
                     ALDLVESDTGYTVTVDLPGVAKEDVKVSIDGRKLTLQAQSKVEKEQKDGERVLYRERS
                     VASYSRSFTLPVEVDQAASQAKLDNGVLTLTLVKKAQPGASQLTVN"
     gene            complement(1647..2678)
                     /gene="tsaD"
                     /locus_tag="VFV25_06055"
     CDS             complement(1647..2678)
                     /gene="tsaD"
                     /locus_tag="VFV25_06055"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEY1392917.1"
                     /translation="MRVLGIESSCDETGVALVEAGAAPVPRLLAHALHSQIEMHQAYG
                     GVVPELASRDHIRRVLPLAQSVMAEAGLGLDALDVVAYTRGPGLAGALLVGAGVACAL
                     GAALGRPVLGVHHLEGHLLSPFLSADPPQFPFVALLVSGGHTQLMRVEEVGRYELLGE
                     TIDDAAGEAFDKSAKLMGLGYPGGPALARLAEFGDAQAFALPRPLLHSGKLDFSFAGL
                     KTAVRVQLGKLGPNVCEQSRADLAAATQAAIVDVLVAKSLAALKLTGLKRLVVAGGVG
                     ANRRLREQLDAQCAKRGVRVHYPELHLCGDNGAMIALAAAMRLQSGKAQGRRDYGFDV
                     RPRWDLSAA"
     gene            2907..3188
                     /locus_tag="VFV25_06060"
     CDS             2907..3188
                     /locus_tag="VFV25_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392918.1"
                     /translation="MKEYKLSGWPELPPPFHRTGYRRMLHEMSQRHVSVDHLMHASGM
                     GRPAVKEFVEMLDARGVLDVHGPAESDSFFGSLWPLEWLRRTREHLEHH"
BASE COUNT          591 a         1156 c         1053 g          512 t
ORIGIN      
        1 agcctgaggc cctcgagcgc gcgggccacg gccaccgcat cggtgctatg cgccttctcc
       61 gtcgcctgcg ccaagagctc gatcatggcc tgcatgcgcg cgtgcacata gtcgtcctcg
      121 ggccggggaa agcgctggcg gaagctctgg tagaagcggt ccgagcccgg cgtgccgaca
      181 ttgggatgcc attcggccac ggccagcacg cggcccacgc cggcctcgcc gatggccgcc
      241 ggcgcaccca atgcgttgcc gtagaaggtg tagaacttcg cgtcgacgcc ggcctcgcgc
      301 gcggccttga tcagcagggt caggtcgttg ccccagttgc cggtcagcac ggcgtcggcg
      361 ccgctggcct tgatcttggc cgcatagggc aggaagtcct tgatgcggcc gatggggtgc
      421 agctcctcgc cgacgacttc gacatccggt cgcttggccg caatcagcgc gcggccctgc
      481 ttcagcacat agcggccgaa gctgtagtcc tggccgatca gatagacctt cttcaccgcc
      541 gcatcgtcgc gcagcgcatc gctcagagcg gccaggcgca tgtcggcatt ggcgtcgaag
      601 cggaaatgcc agaagctgca gttttcgttg gtcagcgccg ggtcgaccgc cgcgtagttg
      661 aaaaacagca cgcgccgatc cggctcgcgc tcattgcgct tgttcaccgc gtcgatcaag
      721 gcggcggcga cggccgagct gttgccctga agcacgacgg cggcaccatc atccgtggct
      781 gccttgagca acgacaaggc ttcgccgaca tcgcccttgc tgtcgtaacg cagcagctgc
      841 agcggacgcg ccccgcccgg cagctgcacg ccaccgcgcg cattgacgcg ctccaccgcc
      901 caggcgagat tgcgccagac cgcctcgccc gcatttccga acggcccgga caagccttcg
      961 atcagcgcca gcctgatcgg ctgggcggca gtcggggcgg ccggtgcagt tgcggccagg
     1021 ccgcacagcg cgaggccgat caacaaggtg cgacgcaatg acggcaagcg aactcccgag
     1081 cccttgaaaa caaaaaagcc gctctcaact cggcggcatg ggttgctgca ttgagcagcg
     1141 ccttgatcaa agattgtagg agttcatcat catgttcctg atcccctctg gccgccaccc
     1201 ggcccgcaat ggccatgccg tgtatttcaa ccgcgccttc gatcgcctct tcgacgatgc
     1261 cttcgatcgc gccttcggcg aggccgcgcc ctcgcgcgcg ccggcgctcg acttggtcga
     1321 gtccgacacc ggctatacag tgaccgtgga cctgcccggc gtcgcgaagg aagacgtcaa
     1381 ggtgtccatc gacggccgca agctcacgct gcaggcgcaa agcaaggtcg aaaaggagca
     1441 aaaggacggc gagcgcgtgc tctatcgcga gcgttccgtt gccagctatt cgcgcagctt
     1501 cacgctgccg gtcgaagtcg accaggccgc ctcgcaggcc aagctcgaca acggcgtgct
     1561 gaccctgacg ctggtcaaga aggcccagcc aggcgcgtcg cagttgaccg tcaactgatg
     1621 acgagcggcc cagaacccac gagggttcag gccgcgctca ggtcccagcg cggccggacg
     1681 tcgaagccgt agtcgcgccg cccttgggct ttgccgcttt gcagccgcat cgcggcggcc
     1741 agcgcgatca tcgcgccgtt gtcgccgcac agatgcagct cggggtaatg cacgcgcacg
     1801 ccgcgcttgg cgcattgcgc gtcgagctgc tcgcgcaagc gccggttggc cccgacgccg
     1861 ccggccacca cgaggcgctt caagccggtg agtttgagcg cagcgagcga cttggcaacc
     1921 agcacgtcga cgatcgcggc ctgggtcgcc gccgcgaggt cggcccgtga ctgctcgcag
     1981 acattgggcc cgagcttgcc cagctgcacg cgcacggcgg tcttgagccc ggcgaaagag
     2041 aaatccagct tgccgctgtg cagcagcggg cgcggcagcg caaacgcctg cgcatcgccg
     2101 aactcggcga gccgcgccag cgccggcccg cccggatagc ccaggcccat cagcttggcc
     2161 gatttgtcga aggcttcgcc ggccgcatcg tcgatggtct cgcccagcag ctcgtagcgg
     2221 ccgacttcct ccacccgcat cagctgggtg tggccgccgg acaccagcag cgcgacgaaa
     2281 ggaaactgcg gcggatcggc cgacaggaag ggcgacagca aatgcccctc gagatggtgc
     2341 acgcccagca ccggccgccc cagcgctgcg cccagggcgc aggccacgcc agcgcctacc
     2401 agcaaggcac cagccaaccc cggcccgcgc gtataggcca ccacatccag cgcatccagg
     2461 cccaggccgg cttcggccat cacgctctgg gcaagcggca gcacgcggcg gatgtggtcg
     2521 cgcgaggcca gctcgggcac gacgccgcca taggcctgat gcatctcgat ctggctgtgc
     2581 aaggcatggg ccagcagacg cggcaccggg gccgcaccgg cctccaccag ggccacgccg
     2641 gtttcgtcgc aggaagattc gatacccaga acacgcatgg gccaagttta gccagcgccc
     2701 gtctgggcct tgaggggctg agccggcccg ctccttgctt gcaatttgat accacgccag
     2761 gctcgccaaa cgtggcgcaa ttgccgaaaa gccttgtagc aagctttcgc ttgtcacacc
     2821 ccagcagtac ccttgcctca caaatcccgg ccatgtcccg aaacgcgagg gccgggcccc
     2881 tcaaaacaaa agacgtcgtt ggagccatga aagagtacaa gctctctggg tggccggaac
     2941 taccgccccc cttccatcgc accggctacc gccgcatgct gcacgagatg tcgcagcgcc
     3001 atgtctcggt ggaccatctc atgcacgcca gcggcatggg gcggccggcc gtcaaggaat
     3061 tcgtcgagat gctcgatgcc cgcggcgtgc tggacgtgca cggcccggcc gagtcggatt
     3121 ccttcttcgg cagcctgtgg ccgctggaat ggctgcgccg cacgcgcgag cacctcgaac
     3181 atcactgagg cgcggcgcca cgaaaacaaa agggctcgca atgcgagccc atttgtttgt
     3241 agcccgcgca taatgtccgg cgctacatat tttgtagtta tattcctcaa aaatagctgg
     3301 gtaggtgtga ta
//
LOCUS       DASUCY010000312         2878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_231493, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000312 DASUCY010000000
VERSION     DASUCY010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231493"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /gene="modC"
                     /locus_tag="VFV25_06065"
     CDS             complement(<1..621)
                     /gene="modC"
                     /locus_tag="VFV25_06065"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HEY1392919.1"
                     /translation="MSLRLARPGFALELDCILPGGGVTALFGPSGCGKTTVLRALAGL
                     ERAKGRVVVNGEVWQDDASKAFVPTHRRAIGYVIQEPTLFPHLDVRRNLVYGLRRIPP
                     QQRRIGMDRALSLLGIGHLLDRAPSSLSGGERQRVAIARALLTSPQLLLMDEPLAALD
                     AARKAEILPYLERLHVELRMPIVYVTHSMEEVARLVDHLVLMESGRV"
     gene            complement(731..1423)
                     /gene="modB"
                     /locus_tag="VFV25_06070"
     CDS             complement(731..1423)
                     /gene="modB"
                     /locus_tag="VFV25_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HEY1392920.1"
                     /translation="MFAPDDWIAIRLTAELAALATVLLLLVGTPLAWWLACTRSRLKP
                     LWSALVAMPLVLPPTVLGFYLLLLMGPHGPLGLVTQALGLGRLPFSFAGLVVASVMYS
                     MPFVVQPLQQAFAAISPRTLEAAATLRAGAGDRFFTIALPLARPGLLTAAVLGFAHTV
                     GEFGVVLMLGGNLPGRTRVLSVALYDHVEAGEFADAHRLAAVMVVFALSVLTTLYIVD
                     PVAREKRQAVAA"
     gene            complement(1426..2193)
                     /gene="modA"
                     /locus_tag="VFV25_06075"
     CDS             complement(1426..2193)
                     /gene="modA"
                     /locus_tag="VFV25_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY1392921.1"
                     /translation="MNTMSWRLLALVTAATFVALPARADEVQVAVAANFAGPLGKIAE
                     GFGAATGHVLKISSGATGKFYSQIRAGAPFEVLIAADDETPKKLVDEGFAVAGSNFTY
                     AIGKLVLWSAKPGFVDAEGKVLAGNGFAHLAIANPKIAPYGAAALEVLKARGLLDTAT
                     PRLVTAESIAQAYQFVSTGNAELGFVALSQVAVPGKPVLGSYWLVPSTLYRQIRQDAV
                     LLKTAENSAAARALLSYLKSAPARAVIESYGYGHEQP"
     gene            complement(2208..>2878)
                     /locus_tag="VFV25_06080"
     CDS             complement(2208..>2878)
                     /locus_tag="VFV25_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEY1392922.1"
                     /translation="GYSYRGAWLLLETAANLAKEPLMERTTGGVHGGGSRLTPTAQAF
                     LAAWRKLQAAHQDFLREQDAWLSQQPVLAGALRRMSMKTTARNQFAGTVTEVTLGTVS
                     AQATIAIGGGQEITATMTMAAAKRIKLAKGREALVLIKSSAVVLITDFAGYVLSARNQ
                     LAGTISRIDKGAVSSLVGVTLPGGAVVTASITNDALEALSLSIGQAATAAFKAYSVMV
                     AVAQ"
BASE COUNT          511 a          983 c          962 g          422 t
ORIGIN      
        1 cacacgaccg gactccatca gcaccaggtg atcgaccaga cgcgccacct cctccatgga
       61 gtgcgtcacg tagacgatgg gcatgcgcag ctcgacgtgc aagcgttcca agtagggcag
      121 gatctcggcc ttgcgcgctg catctagcgc ggccaagggc tcgtccatca gcagcagttg
      181 ggggctggtc agcaaggccc gcgcgatcgc cacgcgctgg cgctcgccgc cggacagcga
      241 gctcggtgct cggtccagca ggtggccgat gcccagcagg ctcaatgccc gatccatgcc
      301 gatgcgcctt tgctgcgggg ggatccttcg caagccgtag acgagattgc ggcgcacgtc
      361 gaggtgcgga aacagcgttg gctcctggat gacgtagccg atggcgcggc gatgcgtggg
      421 cacgaaggcc ttggatgcat cgtcctgcca cacctcgccg ttcaccacta cacgcccctt
      481 ggcccgttcg agccccgcaa gcgcgcgcag caccgtggtc ttgccgcacc ccgaagggcc
      541 gaacagcgcc gtgacgccgc cgccgggcaa gatgcaatcg agctccagcg caaagccggg
      601 gcgagccagg cgcaggctca tttcgagcgt gccgtggcgg gggcccacct gcagctgttg
      661 ggctggcgct tcgacgccga ggccgcacgc ggcttccatc gtcagttccc agggatagtc
      721 catcagagcc tcaggcagcg acagcctggc gcttctcgcg cgcaaccggg tcgacgatgt
      781 agagcgtcgt cagcacgctc aaagcgaaca ccaccatcac ggccgccagg cggtgcgcgt
      841 cggcgaactc gccggcctcg acatggtcgt atagcgccac gctgagcacg cgcgtgcgcc
      901 caggcagatt gccgcccagc atcagcacca ccccgaattc gcccacggtg tgcgcgaacc
      961 ccaagaccgc tgcggtcagc aggccagggc gcgccagcgg caaggcgatg gtgaagaagc
     1021 ggtcaccggc accggcgcgc agggtcgccg ccgcctccag cgtgcgcggc gaaatggctg
     1081 cgaaggcctg ctgcagcggc tgcacgacga aaggcatgga gtacatcact gaagccacca
     1141 ccagaccggc aaagctgaac ggcaggcgcc cgaggcccag ggcctgcgtg acgagcccga
     1201 gcgggccatg cggccccatc agcagcaaca ggtagaagcc cagcacggtg ggcggcagca
     1261 ccagcggcat cgccaccagc gccgaccaca aaggcttcag ccgcgagcgc gtacaggcca
     1321 gccaccacgc cagcggagtc ccgacgagca gcagcagcac cgtcgccagc gcggccagct
     1381 cggcggtgag gcggatcgca atccagtcgt caggcgcgaa catcgtcatg gctgctcgtg
     1441 gccgtagccg taagactcga tgaccgctcg ggcaggtgcg ctcttcagat agctcagcaa
     1501 tgcgcgggcg gccgcgctgt tctctgcggt cttgagcagc acggcgtcct ggcggatctg
     1561 ccggtaaagc gttgacggca cgagccagta cgaacccagc acaggcttac cgggcacggc
     1621 aacctgcgac agcgcgacga agcccagttc ggcattgccg gtggagacga actggtaagc
     1681 ctgggcgatg ctctcggccg tcaccaggcg cggcgttgcc gtgtccagca gtccgcgcgc
     1741 cttcagcacc tccagggccg cggcgccata gggcgcgatt ttggggttgg cgatggccag
     1801 gtgcgcaaag ccgttgcccg cgagcacctt gccctcggca tcgacgaagc cgggctttgc
     1861 gctccacagc accagcttgc cgatggcata ggtgaagttg ctgccggcca cggcgaaccc
     1921 ttcgtccacc agcttcttcg gcgtctcgtc gtcggcagcg atgagcacct cgaagggcgc
     1981 gccggcgcga atctgcgagt agaacttgcc tgtcgcgccg gacgagatct tgagaacatg
     2041 gccggtggcg gcgccgaagc cctcggcgat cttgccgagc ggccccgcga agttcgcagc
     2101 caccgccacc tgcacttcat cggcgcgcgc cggcaatgcg acaaaggtgg ccgccgtgac
     2161 caaagccagc aagcgccacg acatcgtgtt catcacagtg ctcctcctca ttgcgctacc
     2221 gcaaccatga ccgaataggc cttgaacgca gccgtggcag cttgtccgat ggaaagcgac
     2281 agcgcctcaa gcgcgtcgtt ggtgatggag gcggtgacca cagcgccacc tggcagcgtg
     2341 acgccgacca gcgacgagac tgcgcccttg tcgatgcgcg agatcgtgcc ggccagctgg
     2401 ttgcgcgccg acagcacgta gcccgcgaag tccgtgatca gcaccacggc cgaggacttg
     2461 atcagcacga gcgcctcccg ccccttcgcg agcttgatgc gtttggctgc ggccatggtc
     2521 atggtggcgg tgatctcctg tccaccaccg atggcgatgg tcgcctgcgc gctcactgtg
     2581 ccgagcgtga cctcggtaac agtaccggcg aactggttgc gtgccgtggt cttcatggac
     2641 attctccgaa gtgcgccggc caggaccggc tgttgcgaaa gccaggcgtc ctgctcgcgc
     2701 aagaagtcct ggtgcgctgc ctgcagcttt cgccaggcgg caaggaacgc ttgggcggtg
     2761 ggggtcaagc gggaaccgcc gccgtgcaca ccgccggtgg tgcgctccat taggggctcc
     2821 ttggcgagat tggcggccgt ttcgagcaag agccacgcgc cgcggtagct gtagcccg
//
LOCUS       DASUCY010000313         3520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1307742, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000313 DASUCY010000000
VERSION     DASUCY010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1307742"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1469
                     /locus_tag="VFV25_06085"
     CDS             <1..1469
                     /locus_tag="VFV25_06085"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEY1392923.1"
                     /translation="RATIAALSLKVEGDLQTRAEAGDVDARELQRRVGAAWPLQPGTP
                     FGQADWSAAKSAVLVQLRAAGYPLAAWTSTAAEVDAAQHSVAITAVADSGPRFHFGEL
                     RIEGLSRYDESAIRNLAPFDPGTPYSEQALLDFQERIAKAGLFDSVAVTLEPDAAQAS
                     AAPVLVRVRELPKQQATVGVGYSMESGPRLSFEHLQRRPFGLDWQAKTKLQLGRNQSS
                     LSLDLTSHPLPGLYRNLLSGSYTHEVASGVTTNSQQYRVGRTQDTDAIERLYYFEWQH
                     AAINSDGVDSSSTAATLNYEWVWRRLDSNLLPTRGISASAKVGAGRAFQAAGDLQLNA
                     GWFSRASGRVTGYLPLGAQWFGQARLEGAQVFSGPDVAVPYTLLFRAGGDNSVRGYGY
                     QSLGPNNQDGTPAGGHVLATSSIELARPISPKRPAWLGAVFVDAGDAAIDWRSLRPAL
                     GYGVGARWRSPAGLLSLDLAYGQRTRKFRLHFNVGVVF"
     gene            1480..>3520
                     /locus_tag="VFV25_06090"
     CDS             1480..>3520
                     /locus_tag="VFV25_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392924.1"
                     /translation="MPDKASTPPTIRSPRRTALALLAALLLALVGLGAALAWAVASPT
                     GTRTLLSWVPGLRIAGESGALLGNFAAEQAELALPRGGSLVLSRLRWQGLRLEHDDAR
                     AAAWALRLSIASLTAERAELHWVGDPKPNPPPESLKLPLAVDIARLRVGEFRFDPALA
                     PLRELSLGLHLAGNAAGGAAGKESHRIENLSLYWERLQASGELSLGADAPLPLNARFA
                     VKGEADAQADLPAFDAALSVQGPLQHFKLEARLRAEQQALDAQAQVQPFAAWPLAEVN
                     ASTRELDLAALWSAAPHTALSGQVVGHASSPSGKTQPLSLRAELQNGAAGRWDERRLP
                     VRGLSLAIAGDIGSLQQRGELHLELGSARQAGGSLSATGRWNGPTWQLDAQLRDLTPT
                     ALDQRAPAIVLSGPLALNGRGGLPPFGTGSSAGPGEVGLNGRLQGRLLGAPGQGPAGP
                     LALLLDASLSPQRISVRQLEAKAAGASAKLKGEAERVGASAAQPGTWQLRGTLALSDF
                     DPRPWWAAARPQAGDAKPGAAKADARAIAPPPSPPRINADAQFDLRIPEEKAAGRKPR
                     SLLQQLAAWRGQAKAQLRNSQFGGVPLAGELALESHAARQRLLGHARLEAGGNQLTLD
                     GEIDATATASDRWKLQLQAPKLDSLAPLLRLLRPASPPQTSAGSLSAQAEAEGRWP"
BASE COUNT          521 a         1287 c         1247 g          465 t
ORIGIN      
        1 cgcgcgcgac gatcgccgcg ctctcgctga aggtcgaggg tgatctgcag acgcgtgccg
       61 aggcgggcga cgtggacgcc cgcgaactgc agcgacgcgt cggtgcggcc tggccgctgc
      121 agccgggaac accgttcggc caggccgatt ggagcgccgc caagtccgcc gtgctggtgc
      181 agttgcgcgc cgcgggctat ccgctggcgg cgtggacgag cacggcagcc gaggtcgacg
      241 cggcacagca cagcgttgcc atcacggccg tggccgacag cggcccgcgc tttcacttcg
      301 gcgagttgcg catcgagggt ctgtcgcgct acgacgagag cgcgatccgc aacctcgcgc
      361 ccttcgatcc gggcacgccc tatagcgagc aggcgctgct ggacttccag gagcgcatcg
      421 ccaaggcagg cctgttcgac agcgtcgcgg tgacgctgga acccgatgca gcccaagcat
      481 cggccgcgcc ggtgctggtg cgcgtgcgcg agctgcccaa gcagcaggcc acggtgggcg
      541 tgggctacag catggaaagc gggccgcgct tgagcttcga gcatctgcag cgccgcccct
      601 tcgggctgga ctggcaggcc aagaccaagc tgcagctggg ccgcaaccag agctcgctga
      661 gcctggacct cacctcccac ccgctgccgg gcctgtaccg caaccttctg tcgggcagct
      721 acacgcacga ggtcgcctcg ggtgtgacga ccaacagcca gcaataccgc gtcggccgca
      781 cccaggacac cgacgcgatc gagcgcctct actacttcga gtggcaacac gccgcgatca
      841 acagcgacgg cgtggacagc agctcgaccg ccgcgacgct gaactacgaa tgggtctggc
      901 gccggctcga cagcaatctg ctgcccacgc gcggaatcag cgcatcggcc aaggtcggtg
      961 cgggccgcgc cttccaggcg gccggcgacc tgcagctcaa tgccggctgg ttctcgcgcg
     1021 ccagcgggcg ggtcaccggc tacctgccgc tgggcgcgca atggttcggc caggcgcggc
     1081 tggagggggc ccaagtcttc agcggcccgg acgtggccgt gccctatacc ctgctgttcc
     1141 gtgccggcgg cgacaactcg gtgcgcggct acggctacca gtcgctgggc cccaacaacc
     1201 aggacggcac accggccggc ggccatgtgc tggccacctc cagcatcgag ctggcgcggc
     1261 ccatctcgcc taaacggccg gcctggctgg gcgccgtctt tgtcgatgcc ggcgacgcgg
     1321 caatcgactg gcgcagcctg cgcccggcgc tcggctatgg cgtcggcgcg cgctggcgca
     1381 gcccggcggg tctgctgagc ctggacctgg cctatggcca gcggacgcgc aagttcaggc
     1441 tgcacttcaa cgttggcgtc gtgttctagc ttctgagcca tgcctgacaa ggccagcacc
     1501 ccacccacca tccggagccc tcgccgcacc gcgctagcgc tgctcgccgc gctgctgctg
     1561 gcgctggtcg gcctgggcgc ggcgctggcc tgggcggtgg cgagccccac gggcacgcgc
     1621 accctgctgt cctgggtgcc gggcctgcgc atcgccggcg agagcggcgc gctgctgggc
     1681 aacttcgcgg ccgagcaggc cgagttggcc ttgccgcgcg gcggctcgct agtcttgagc
     1741 cggctgcgct ggcagggcct gaggctggag cacgacgatg cgcgggccgc agcctgggcg
     1801 ctgcgcctga gcatcgccag cctcaccgcc gaacgcgccg agctgcattg ggtgggcgac
     1861 cctaagccca acccgccgcc cgagagcctg aagctgccgc tggcggtgga catcgcccgg
     1921 ctgcgcgtcg gcgagttccg cttcgaccct gcgttggcgc cgttgcgcga gctgagcctc
     1981 ggcctgcacc tcgcaggaaa tgctgcaggg ggtgcggcag gcaaagagtc gcatcgcatc
     2041 gagaacctga gcctgtattg ggagcgcctg caggccagcg gcgagctgag cctgggcgcc
     2101 gacgcgccgc tgccgctgaa tgcccggttc gccgtgaaag gcgaggccga tgcccaagcc
     2161 gacctgccgg ccttcgacgc agcgctctcg gtccagggcc cgctgcagca tttcaagctg
     2221 gaggcccgcc tgcgtgcgga gcagcaagcc ttggatgccc aggcccaggt ccagcccttt
     2281 gcagcctggc cgctcgctga ggtgaacgcg agtacgcggg agctggatct cgcggcgctg
     2341 tggtcggccg caccgcacac tgctctgagc gggcaagtcg tcggccacgc ctcgtccccg
     2401 agcggaaaaa cccagccgct gagcctgcgt gccgagctgc agaacggcgc cgccggccgc
     2461 tgggacgagc gcaggctgcc cgtacgcggc ctcagcctgg ctatcgccgg cgacatcggc
     2521 agcctgcagc agcgcggcga actgcacctg gaactcggca gcgccaggca ggctggcggc
     2581 agcctcagcg ccaccggccg ttggaacggc cccacctggc agctggacgc gcagctgcgg
     2641 gacctcacgc ccacggcgct ggatcaacgc gcgccggcca ttgtgctgag cgggccgctc
     2701 gcgctgaacg gccggggcgg gctgccgcct ttcggcacag ggagttcggc cggcccgggc
     2761 gaagtcgggc tgaacgggcg cttgcagggc cgcctgctcg gcgcgccggg acaaggcccg
     2821 gccgggccct tggcgctgtt gctcgatgcc agcctgagtc cgcagcgcat cagcgtgcgg
     2881 caactggaag cgaaggctgc cggagccagc gccaagctca aaggcgaagc cgagcgcgtc
     2941 ggcgcctccg ccgcccagcc gggcacttgg cagctgcgcg gcacgctggc cttgtccgac
     3001 ttcgaccccc gcccctggtg ggctgctgca agaccgcagg ccggcgatgc aaagccaggc
     3061 gctgcaaagg ccgacgccag ggcaatcgcg ccaccaccat cgccgccgcg catcaacgcg
     3121 gacgcgcaat tcgacctgcg catccccgaa gaaaaggcag ccggacgcaa gccgcgcagc
     3181 ctgctgcaac agctcgcggc ctggcgcggc caggccaagg cgcagctgcg caacagccag
     3241 ttcggtggcg tgccgctggc cggcgagctc gcgctggaaa gccacgcggc acgtcagcgc
     3301 ctgctcggcc acgccaggct ggaggcgggt ggcaaccagc tgactctgga cggggaaatc
     3361 gacgccaccg ccactgccag cgaccgctgg aagctgcagt tgcaggcccc caagctcgac
     3421 agccttgccc cgctattgcg cctgctgcgg cctgcctcgc cgccccagac ttccgccggg
     3481 tccctgagcg cccaggccga ggccgagggc cgctggccct
//
LOCUS       DASUCY010000314         2623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_408201, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000314 DASUCY010000000
VERSION     DASUCY010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408201"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VFV25_06095"
     CDS             complement(<1..205)
                     /locus_tag="VFV25_06095"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEY1392925.1"
                     /translation="MRLEVPAQKKFTHEMIIPIRWGDMDAMGHVNNTVYFRYLEIIRL
                     DWLYRVGGPPDPEGEGPVIANTFC"
     gene            complement(202..864)
                     /locus_tag="VFV25_06100"
     CDS             complement(202..864)
                     /locus_tag="VFV25_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012367092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HEY1392926.1"
                     /translation="MSTRSLTPLEARIVAVLLEKQHTVPDSYPLSLNALLMGCNQKTA
                     REPVMNLSEAEVLGALDELRDLSLVVEVSGSRVARYEHNLQRVLGVPSQSAALLAVLM
                     LRGPQTAAELRQNSERLHRFADISSVEAFLAELAEADPPKVVKLPRASGEREARWAHL
                     LCGEVQVPAEAGGSDSVAPSELAALRREQQRLSDEVEALRGLVERLYAELGVSKPDEV
                     AG"
     gene            complement(874..1656)
                     /locus_tag="VFV25_06105"
     CDS             complement(874..1656)
                     /locus_tag="VFV25_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY1392927.1"
                     /translation="MSYSIDLSGRVALITGASSGLGAQFARTLSKAGAAVVLAGRRVE
                     RLKTLRSEIEGAGGDAHVVGLDVTDLTSIRSAVAHAETEVGAIDILVNNSGVSTTQKL
                     TEVSEEDYDYVLDTNTKGAFFVAQEVGKRMLARAKGAAPGTFTGGRIVNIASMAGLRV
                     LGQIGIYCMSKAAVIHMTRAMALEWGRYGINVNAICPGYIDTEINHEHWETEQGRKLI
                     EMLPRRRLGQPEDLDALLVMLCANESHFVNGAVIQADDGFAV"
     gene            1860..>2623
                     /locus_tag="VFV25_06110"
     CDS             1860..>2623
                     /locus_tag="VFV25_06110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY1392928.1"
                     /translation="MTPQEILEQYGPREAMEYDVVIVGAGPAGLATAIRLKQLNSELS
                     VVVLEKGSEPGAHILSGAVMDPRAMNELFPNWKELGAPLNQPVTGDDMLFLSETGAKR
                     TPDWLMPRNFHNEGCYVVALGNVVKWMAQQAESLGVEIFPGFAAAEVLYDEQGRVKGV
                     ATGNLGLGKDGQPTDHFQLGMELLGKYTIFAEGARGHLGKQLLAQYKLTEGKDPQSFA
                     IGIKELWEIDPAKAKPGLVVHTAGWPMTDDTFGGGFL"
BASE COUNT          432 a          905 c          855 g          431 t
ORIGIN      
        1 tgcagaaggt gttggcgatc accggtcctt cgccctctgg atcgggcggc ccgccgacgc
       61 gatagagcca gtcgaggcgg atgatctcca gatagcgaaa gtagaccgtg ttgttgacat
      121 ggcccatcgc gtccatgtcg ccccagcgta tcgggatgat catctcgtgc gtgaatttct
      181 tctgcgccgg gacttcgagt ctcatcctgc gacctcgtcc ggtttgctca cgccgagctc
      241 ggcatagagg cgctccacca ggccgcgcag cgcttccacc tcatctgaaa gccgctgctg
      301 ctcgcggcgc agcgccgcca gctcggaggg cgccacgctg tcgctgccgc cggcctcggc
      361 cggcacctgc acctcgccgc acagcaggtg ggcccagcgc gcctcgcgct cgccggaggc
      421 gcgcggcagc ttcaccacct tgggcggatc ggcctcggcc agctcggcga gaaaggcctc
      481 caccgaggag atgtcggcaa acctgtgcag gcgctcgctg ttctgcctca gctcggccgc
      541 ggtctgcggg ccgcgcagca tcagcaccgc gagcagcgcc gccgactggc tgggcacacc
      601 gagcacgcgc tgcaggttgt gctcgtagcg cgccacgcgg ctgccgctga cctcgaccac
      661 gaggctgaga tcgcgcagtt cgtcgagcgc gcccagcacc tcggcctcgc tcagattcat
      721 caccggctcg cgcgccgtct tctggttgca gcccatcagc agggcattga gcgacagcgg
      781 ataggaatcc ggcactgtgt gctgcttctc gagcagcacc gcgacgatgc gggcctccag
      841 cggcgtcagg ctcctggtgg acatgctcga acgctagacg gcgaagccgt catcggcctg
      901 gatcaccgcg ccgttgacga aatggctctc gttggcacac aacatcacga gcagcgcgtc
      961 caggtcctcg ggctggccca ggcggcggcg cggcagcatc tcgatcagct tcctgccctg
     1021 ctcggtttcc caatgctcgt ggttgatctc ggtgtcgatg tagccggggc agatcgcgtt
     1081 gacattgatg ccgtagcggc cccattccag cgccatggcc cgcgtcatgt ggatcaccgc
     1141 agccttgctc atgcagtaga tgccgatctg gcccagcacg cgcaggcccg ccatcgaggc
     1201 gatgttgacg atgcgcccgc cggtgaaggt gcccggtgcc gcccccttgg cgcgcgccag
     1261 catgcgcttg cccacctcct gggcgacgaa gaaggcgccc ttggtgttgg tgtccagcac
     1321 gtagtcgtag tcttcctcgc tcacctcggt gagcttctgc gtggtgctga cgcccgagtt
     1381 gttcacgagg atgtcgatcg cgccgacctc ggtttcggca tgggccacgg ccgagcggat
     1441 gctggtgagg tcggtcacgt ccagccccac cacatgggca tcgccgccgg cgccctcgat
     1501 ctcggagcgc agggtcttga gccgctcgac gcggcggccc gccagcacca cggccgcgcc
     1561 ggccttggac agcgtgcgcg cgaactgggc gcccagcccg ctggatgcgc cggtgatcag
     1621 ggccacgcgg ccggagaggt cgatgctgta gctcatggtg ctgctcctca tgagagcgcc
     1681 agttctttcg gcgcatcttg tgtcgcggcg acgatagcac gcgtgcaagg ccggcggctt
     1741 cgcctgcgcg acagacaggc gagaacgctc gctccaaaaa tagaacgatc gttctatttg
     1801 cagtggccgc cgcttagaat cccgcgcaaa ctccgctttc cacgagacaa ggaaacacca
     1861 tgacgccgca ggagatcctc gaacaatacg gcccgcgaga ggccatggaa tacgacgtcg
     1921 tgatcgtcgg cgccggccct gcaggactgg ccaccgcgat ccggctgaag cagctcaaca
     1981 gcgagctttc ggtcgtggtg ctggagaaag gctccgagcc cggcgcgcac atcctctccg
     2041 gcgcggtgat ggacccgcgc gcgatgaacg agctcttccc caactggaag gagctgggcg
     2101 ccccgctgaa ccagccggtc accggcgacg acatgctgtt cctgtccgag accggcgcca
     2161 agcgcacgcc cgactggctg atgccgcgca acttccacaa cgagggctgc tatgtcgtcg
     2221 cgctgggcaa cgtcgtgaag tggatggcac agcaggccga gagcctgggc gtcgagatct
     2281 tccccggctt cgctgcagcc gaagtgctct atgacgagca aggccgcgtc aagggcgtgg
     2341 ccaccggcaa cctggggctg ggcaaggacg gccagcccac cgaccacttc cagctcggca
     2401 tggagctgct cggcaaatac accatcttcg ccgagggcgc gcgcggccac ctgggcaagc
     2461 agctgctggc ccagtacaag ctcaccgagg gcaaggatcc gcagagcttt gccatcggca
     2521 tcaaggagct gtgggaaatc gacccggcca aggccaagcc cggcctcgtg gtgcacaccg
     2581 ccggctggcc gatgaccgac gacaccttcg gcggcggctt cct
//
LOCUS       DASUCY010000315         2015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_906170, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000315 DASUCY010000000
VERSION     DASUCY010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_906170"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /gene="ribD"
                     /locus_tag="VFV25_06115"
     CDS             <1..702
                     /gene="ribD"
                     /locus_tag="VFV25_06115"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEY1392929.1"
                     /translation="ARELNIGFFSRMIRQRPWLRMKVAASLDGITALDNGASQWITSE
                     AARTDGHRWRARAGAVLTGVGTVREDDPRLDVRLVPAQRQPMRVIVDSRLETPRQARI
                     LAPPGEVLIYYASATGQDAEQFQTLGTQLIALQNQAGKVDLAAMLEDLARRGVNELHV
                     EAGYRLNGSLLREGLVDEFLIYLAPKLLGGGRGMASFGPLQRLQDAVGLEILGVERIG
                     EDLRIIAKPPGRAAF"
     gene            764..1900
                     /gene="ribBA"
                     /locus_tag="VFV25_06120"
     CDS             764..1900
                     /gene="ribBA"
                     /locus_tag="VFV25_06120"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEY1392930.1"
                     /translation="MAISPVPELIAELAAGRMVILVDEEDRENEGDLVLAADHVTPEA
                     INFMAKYGRGLICLTLTRERCERLQLPPMTQRNGAQHGTAFTVSIEAAEGVTTGISAA
                     DRAHTVKVAVAREARARDLVQPGHVFPLQAQDGGVLMRAGHTEAGCDLAGMAGLTPAS
                     VICEIMKDDGTMARLPDLEAFAREHGLKIGTIADLIEYRSRNESLIEHLGQRIIETAQ
                     GRFDCRAFRDRTGGLHLALSLGQWAPHDEVLVRVHEPLSVMDLLDAGPCAHSWPIPRA
                     LAALQAEGRGVAILLNCGEDREGLMQHFLPKEAIASAPRSQMDLRTYGVGAQILRELG
                     ITRMKLLGSPRRMPSMAGYGLEVTAFVSPSDETCAPAKPALRAL"
BASE COUNT          340 a          712 c          672 g          291 t
ORIGIN      
        1 gcgcgcgagc tgaatatcgg cttcttctcg cgcatgatcc ggcagcgccc ctggctgcgc
       61 atgaaggtcg cggcctcgct ggacggcatc accgcgctgg acaacggcgc cagccaatgg
      121 atcacctccg aggccgcgcg caccgatggc caccgctggc gtgcacgcgc cggcgcggtg
      181 ctgaccggcg tcggcaccgt gcgcgaagat gatccgcggc tggacgtgcg cctggtgccc
      241 gcgcagcgcc agccgatgcg cgtgatcgta gattcgcggc tggagacccc gcgccaggcg
      301 cgcatcctcg cgccgcccgg tgaggtgttg atctactacg cctctgccac cgggcaggat
      361 gcagaacaat tccagacact cggtacccag ctcatcgcgc tgcagaatca ggcaggcaag
      421 gtcgacctgg ctgcgatgct ggaagacctg gcccggcgcg gcgtcaacga gctgcatgtc
      481 gaagccggct accggctcaa cggctcgtta ttgcgcgagg gcctggtcga tgaattcctg
      541 atctacctgg cacccaagct gctgggcgga ggacgaggca tggccagctt cgggccgctg
      601 cagcgcctgc aggacgcggt cgggctggaa atcctcggcg tcgagcgaat cggcgaagac
      661 ctgcgcatca tcgccaagcc gccgggacgc gcggcgttct aaggcagccc gacgcaatcc
      721 ctccatcggg aggggccgaa aaggccccgc ctacaatccg aggatggcta tctcccctgt
      781 cccagagctc atcgccgagc tggctgccgg ccgcatggtc atcctcgtcg acgaggaaga
      841 ccgcgagaac gagggcgacc tcgtgctggc ggccgaccat gtcacgccgg aggccatcaa
      901 tttcatggcc aagtacgggc gcgggctgat ctgcctcacg ctcacgcgcg agcgctgcga
      961 gcggctgcag ctgccgccca tgacccagcg caacggcgcc cagcatggca cggccttcac
     1021 cgtatcgatc gaggccgccg aaggcgtgac gaccggcatc tcggccgccg accgtgcgca
     1081 caccgtcaag gtcgcggtgg cgcgcgaagc ccgagcgcgc gacctggtgc agccgggcca
     1141 tgtcttcccg ctgcaggccc aggacggagg cgtgctgatg cgcgccggcc ataccgaagc
     1201 cggctgcgac ctggccggca tggccgggct cacgccggcc tcggtgatct gcgagatcat
     1261 gaaggacgac ggcaccatgg cccgcctgcc ggacctcgag gccttcgcgc gcgagcatgg
     1321 cttgaagatc ggcaccatcg ccgacctgat cgaataccgc agccgcaacg aatccctgat
     1381 cgagcacctg ggccagcgca tcatcgagac agcgcagggg cgcttcgact gccgcgcctt
     1441 ccgcgaccgc actggcggcc tgcacctcgc cctgtcgctg ggccaatggg cgccgcacga
     1501 cgaagtgctg gtgcgcgtgc acgagccgtt gtcggtgatg gatctgctcg atgccggccc
     1561 ttgcgcccat tcctggccca tcccccgcgc gcttgccgcg ctgcaggccg agggccgcgg
     1621 tgtggcgata ctgctcaatt gcggcgagga ccgagagggc ctcatgcagc atttcctgcc
     1681 gaaagaggcc atcgcctccg cgccgcgcag ccagatggat ctgcgcacct atggcgtcgg
     1741 cgcccagatc ctgcgcgagc taggcatcac gcgcatgaag ctgctcggca gcccgcggcg
     1801 catgcccagc atggcgggct atggcctgga ggtgacggcc ttcgtctcgc cctccgacga
     1861 aacctgcgcg ccggccaagc cggccctgcg ggcgctctga acccgcgctc caacacaaca
     1921 acatcggaac ctagggaaga ttactcatgc aaggcgcaga ccgaggccaa gcggccgatc
     1981 tcgacggcga agacctgcgc ataggtgtcg ttcag
//
LOCUS       DASUCY010000316         4286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1516761, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000316 DASUCY010000000
VERSION     DASUCY010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4286
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1516761"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1253)
                     /locus_tag="VFV25_06125"
     CDS             complement(<1..1253)
                     /locus_tag="VFV25_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrpR N-terminal domain-containing protein"
                     /protein_id="HEY1392931.1"
                     /translation="MAVSRHRLKGLLEELAPEYSERARVEVLDKGYQEAVAEVRALQA
                     SEPVDVLVAAGSNGAFLRQHLDLPVVLVKVGGFDVMRALGRARAISSRIAIVTYGEPP
                     PEVQQFNELFGLGVELRSYRSEEDAGACVRELRSRGIEVVVAPGLVADLADELGMLGV
                     FLYSQDAVREALDDAVEIARASRIELTKRERLNTILAQLKDGVVAVDMQERIETLNPA
                     MAQLLGAPAEALLGRRLSEVAPELSLAATLRDARAEPEQIQRYGQRRLVASRMPIVEQ
                     GLQTGAVLSCQDPAAIHRMDRSLRASHPPRGASRTTRYELEQIVGVSPAIRRAKALAA
                     ACAASDATVLISGESGTGKELLAQGIHHASRRRRQPFVAVNCAAFPESLLESELFGYE
                     EGAFTGSRRGGKAGLFEAAHTGTIFL"
     gene            1533..2420
                     /gene="prpB"
                     /locus_tag="VFV25_06130"
     CDS             1533..2420
                     /gene="prpB"
                     /locus_tag="VFV25_06130"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018025787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HEY1392932.1"
                     /translation="MSKPSSPGARFRAALQEESPLQVIGAINANHALLAKRAGYRAIY
                     LSGGGVAAGSLGMPDLGINTLDDVLTDVRRITDVCELPLMVDIDTGFGPSAFNIERTI
                     KSLIKFGAAACHIEDQVGAKRCGHRPGKEIVSMDEMVDRVKAAADAKTDPDFFLIART
                     DAIQMEGVDAAIERACRCVEAGADGIFAEAAYDLPTYRRFVDAVKVPVLANITEFGKT
                     PLFTVEELKSADVAMVLYPLSAFRAMNKAAETVYQAIRRDGHQKNVLELMQTREELYD
                     RIGYHEFESKLDALFAAKK"
     gene            2437..3591
                     /gene="prpC"
                     /locus_tag="VFV25_06135"
     CDS             2437..3591
                     /gene="prpC"
                     /locus_tag="VFV25_06135"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HEY1392933.1"
                     /translation="MTDTAAPGFKPKKSVALSGTAAGNTALCTVGRTGNDLHYRGYDI
                     LDVATACEFEEIAHLLVHGKLPNAAELAGYKAKLKALRGVPAAVKAVLEQLPPSAHPM
                     DVMRTAVSTLGCIAPEKDDHNHPGARDIADRLMASLGSCLLYWYHYSHNGKRIDVETD
                     DDSIGGHFLHLLHGKKPSDLWVRAMHTSLNLYAEHEFNASTFTARVIAGTGSDMHSAI
                     AGAIGALRGPKHGGANEAAFEVQKRYDTPDEAEADIRARVERKEVVIGFGHPVYTVSD
                     PRNKVIKEVARELSQEAKSMKMFSIAERLESVMWDIKKMFPNLDWFSAVSYHMKGVPT
                     AMFTPLFVISRTSGWSAHVIEQRIDGKIIRPSANYVGPEDLKFVPLKSRP"
     gene            3612..>4286
                     /locus_tag="VFV25_06140"
     CDS             3612..>4286
                     /locus_tag="VFV25_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015878151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEY1392934.1"
                     /translation="MNTTHRKPLPGTQLDYFDARTAVDAIQPGAWSKLPYTSRVHAEN
                     LVRRAEPAMLDAYLRQLIERKRDLDFPWFPARVVCHDILGQTALVDLAGLRDAIAEKG
                     GDPAKVNPVVPVQLIVDHSLAVEHGGFEKDAFAKNRAIEDRRNDDRFHFINWTREAFQ
                     NMQVIPPGNGIMHQINLERMSPVIYTQDGVAFPDTCVGTDSHTPHVDALGVIAVGVGG
                     LEAENVM"
BASE COUNT          742 a         1500 c         1406 g          638 t
ORIGIN      
        1 aggaagatgg tgccggtgtg cgccgcctcg aacagcccgg ccttgccgcc gcgccgcgag
       61 ccggtgaagg cgccttcctc gtagccgaat agctcgctct cgagcagact ttccggaaag
      121 gccgcgcaat tcaccgccac gaagggctgg cggcggcggc ggctcgcgtg gtggatgccc
      181 tgggcgagca attccttgcc ggtgccgctc tcgccgctga tcagcaccgt ggcatcgctg
      241 gcggcgcagg cggcggccag ggccttggcg cggcggatcg ccgggcttac gcccacgatc
      301 tgctccagtt cgtagcgcgt cgtgcggctc gcgccgcgcg gcggatggct ggcgcgcagg
      361 ctgcggtcca tgcggtggat ggcggccggg tcctggcaac tcagcacggc gccggtctgc
      421 aggccctgct cgacgatggg catgcggctc gccacgagcc ggcgctggcc atagcgctgg
      481 atctgctcgg gctcggcgcg cgcgtcgcgc agcgtggcgg cgaggctcag ctcgggcgcg
      541 acctcgctca ggcgccgccc cagcagggcc tcggccggcg cgcccagcag ctgcgccatc
      601 gccgggttca gcgtctcgat gcgctcctgc atgtccaccg ccaccacgcc gtccttgagc
      661 tgggcgagga tggtgttgag gcgctcgcgc ttggtcagct cgatgcgcga ggcgcgcgcg
      721 atctccaccg catcgtcgag cgcttcgcgc accgcatcct gcgaatagag aaacacgccc
      781 agcatgccca gctcgtcggc caggtcggcc accagtccgg gtgctaccac cacctcgatg
      841 ccgcgcgagc gcagctcgcg cacgcaggcg ccggcgtctt cctcgctgcg gtaggagcgc
      901 agctccacgc ccaggccgaa cagctcgttg aactgctgca cctcgggcgg aggctcgcca
      961 taggtcacga tcgcgatgcg cgaggagatg gcccgggcgc ggccgagcgc gcgcatcacg
     1021 tcgaagccgc ccaccttcac cagcaccacc ggcaggtcga ggtgctggcg caggaaggcg
     1081 ccgttcgagc cggccgccac cagcacatcg accggctcgc tggcctgcag ggcgcgcacc
     1141 tcggcgaccg cctcctgata gcccttgtcg agcacctcga cgcgggcccg ctcgctgtac
     1201 tccggcgcca gttcctccag caggcccttg agccggtgcc ggctgacggc cacgatgcga
     1261 ggctggccgc ggggcggggg taggatgggt ctaggagcgg gtttcatagc gcgccgcctg
     1321 ttcaaaggtg aaacgagtga atcataatct gttcaccaat gaaacataag agcgctcacc
     1381 cctgaaacct gttgcgcggc cgggcgggga tttctctcgt gatatcaatg acttgcagcc
     1441 gccacccgga atcgctgcgg tgccgccttg tgatggcatg cgcattgcac tgagacaggt
     1501 attccgtttc acctcgcaaa ggacacgcga acatgagcaa gcccagctcc cccggcgccc
     1561 gcttccgcgc cgcgctgcag gaggaatcgc cgctgcaggt gatcggcgcc atcaacgcca
     1621 accacgcgct gctcgccaag cgcgcgggct accgcgcgat ctatctctcg ggcggcggcg
     1681 tggcggcggg gtcgctgggc atgcccgacc tcggcatcaa cactctcgac gacgtgctca
     1741 ccgatgtgcg ccgcatcacc gacgtgtgcg agctgccgct gatggtggac atcgacaccg
     1801 gcttcgggcc cagcgccttc aacatcgagc gcacgatcaa gagcctcatc aagttcggcg
     1861 ccgcggcctg ccacatcgaa gaccaggtcg gcgccaagcg ctgcggccat cgccccggca
     1921 aggagatcgt ctccatggac gagatggtcg accgcgtgaa ggccgcggcc gacgcgaaga
     1981 ccgacccaga cttcttcctc atcgcgcgta ccgacgcgat ccagatggag ggcgtggatg
     2041 ccgccatcga gcgcgcttgt cgctgcgtgg aggccggagc cgacggcatc ttcgccgagg
     2101 cggcctacga cctgccgacc taccggcgct tcgtcgacgc ggtcaaggtg ccggtgctgg
     2161 ccaacatcac cgagttcggc aagaccccgc tgttcacggt ggaagaattg aagagcgccg
     2221 acgtggccat ggtgctctac ccgctgtcgg ccttccgcgc gatgaacaag gccgccgaga
     2281 ccgtgtacca ggccatccgc cgcgacggcc accagaagaa cgtcctcgag ctgatgcaga
     2341 cgcgcgagga gctctacgac cgcatcggct atcacgaatt tgagagcaag ctggacgcac
     2401 tgtttgcggc caagaagtga gattcaggag acaacgatga ccgacaccgc cgctcccggc
     2461 ttcaagccca agaaatccgt cgcactctcg ggcaccgccg ccggcaacac ggcgctgtgc
     2521 accgtgggcc gcaccggcaa cgacctgcac taccgcggct acgacatcct ggacgtggcc
     2581 accgcctgcg agttcgagga gatcgcccac ctgctggtgc acggcaagct gcccaacgcg
     2641 gccgagctgg ccggctacaa ggccaagctc aaggccctgc gcggcgtgcc ggcggccgtg
     2701 aaggcggtgc tggagcagct gccgccctcg gcccatccga tggacgtgat gcgcacggcg
     2761 gtctcgaccc tgggctgcat tgcgcccgag aaggacgacc acaaccaccc cggcgcgcgc
     2821 gacatcgccg acaggctgat ggcctcgctc ggctcgtgcc tgctgtactg gtaccactac
     2881 agccacaacg gcaagcgcat cgacgtcgaa accgacgacg actcgatcgg cggccacttc
     2941 ctgcacctgc tgcacggcaa gaagcccagc gatctgtggg tgcgtgcaat gcacacctcg
     3001 ctgaacctct atgccgagca cgagttcaac gcgtctacct tcacggcccg cgtgatcgcc
     3061 ggcaccggct ccgacatgca ctcggccatc gccggcgcca tcggcgcgtt gcgcgggccc
     3121 aagcatggcg gcgccaacga ggcggccttc gaggtccaga agcgctacga cacacccgac
     3181 gaagccgagg ccgacatccg cgcgcgcgtc gagcgcaagg aagtggttat cggattcggc
     3241 catccggtct acacggtcag cgacccgcgc aacaaggtca tcaaggaggt ggcgcgcgag
     3301 ctgtcgcagg aggccaagag catgaagatg ttcagcatcg ccgagcgcct ggagtccgtc
     3361 atgtgggaca tcaagaagat gttccccaac ctcgactggt tctcggccgt gagctatcac
     3421 atgaagggtg tgcctaccgc gatgttcacg ccgctcttcg tgatctcgcg caccagcggc
     3481 tggagcgcac acgttatcga gcagcgcatc gacggcaaga tcatccgccc gagcgccaac
     3541 tatgtcgggc ccgaggatct gaagttcgtg ccgctgaaaa gccgtccctg aagccttgag
     3601 gaccgttcgt catgaacacc acccaccgca agccgctgcc cggcacgcag ctggactact
     3661 tcgatgcacg cactgcggtc gatgcgatcc agcccggcgc ctggagcaag ctgccctaca
     3721 cctcgcgcgt gcatgccgag aacctggtgc gccgagccga gccggccatg ctcgatgcct
     3781 atctgcggca gctgatcgag cgcaagcgcg atctcgactt cccctggttc ccggcgcgcg
     3841 tggtctgcca tgacatcctg ggccagaccg cgctggtgga cctggccggc ctgcgcgatg
     3901 cgatcgcgga aaaaggcggc gacccggcca aggtgaaccc ggtggtgccg gtgcagctga
     3961 tcgtcgacca ctcgctggcg gtggagcacg gcggcttcga gaaggacgcc ttcgccaaga
     4021 accgcgcgat cgaggaccgc cgcaacgacg accgcttcca tttcatcaac tggacccgcg
     4081 aggcgttcca gaacatgcag gtgatcccgc cgggcaacgg catcatgcac cagatcaacc
     4141 tggagcggat gtcgccggtg atctacacgc aggacggcgt ggccttcccc gacacctgcg
     4201 tcggcaccga cagccacacg ccacacgtcg atgcgctggg cgtgatcgcg gtcggcgtgg
     4261 gcggcctgga ggccgagaac gtgatg
//
LOCUS       DASUCY010000317         2718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1050981, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000317 DASUCY010000000
VERSION     DASUCY010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050981"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1099
                     /locus_tag="VFV25_06145"
     CDS             <1..1099
                     /locus_tag="VFV25_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011004329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY1392935.1"
                     /translation="DTLASPASSGASVYNLHTLQLTVSYTPDVFGGNRRQVETLQAQA
                     DSQRWEAEATYLALTSNVTAAAVQEASLRGQIQATRELIDIQTEILKRFQRQYQLGQV
                     ALADVTQQEAALAAVQATLPPLDKQLALQRDLIKALAGQLPSEHLDAEFTLDSLSLPA
                     ELPLTLPSTLVDHRPDVLAAEEQLHIASANIGVARAARLPSVQLGVNAYGSAAYSIPD
                     LFKAGTIFWTLAGGVAQTVFDGGTLRHREAAAAAAYDQAAAQYRATVINAYQNVADAL
                     QAIQADAQALRAADYAQDAADRSLAIARRQLALGDVSPMFVMQAEQAALQARLSVVQA
                     RANRLSDSVALFQALGGGWWNRQESLTQAPQ"
     gene            1110..1475
                     /locus_tag="VFV25_06150"
     CDS             1110..1475
                     /locus_tag="VFV25_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY1392936.1"
                     /translation="MEIQRHEMGPRFSEMTVLRIGDAKLIYIAGQVAENTSLEVAGQT
                     REVLGVIDRLLAQAGATKADIVSARIYLASVGDYATMNSVWDDWVPPGHTPSRATIGA
                     KLINPEYKIEIEVTAAVQA"
     gene            1480..1974
                     /locus_tag="VFV25_06155"
     CDS             1480..1974
                     /locus_tag="VFV25_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEY1392937.1"
                     /translation="MEHPGKTRAVRGFTLMELMVVIAVIAILATIALPNLQGRLVRGQ
                     VVEAMKLADIAKGPVAARWSSTKTLPPDNASVGLPSADKIVSNYVSSLSVEGGAIQVT
                     FGNNVNGAIKGKTLSLRPAVVEDAPVVPVAWVCGSAPVPDKMTAMGANKTDLPPAFLP
                     LNCR"
     gene            complement(2066..>2718)
                     /locus_tag="VFV25_06160"
     CDS             complement(2066..>2718)
                     /locus_tag="VFV25_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY1392938.1"
                     /translation="VEWLTTTYGGVDYLVNNAGRSIRRAIESSYDRFHDYERTMQLNY
                     FGCLRVTMGLLPGMVAKRKGHVVNISSIGVLTNAPRFSAYVASKAALDAWTRCASSEL
                     ADMGVTFTTINMPLVRTPMIAPTKIYNNVPTLSPEEAADMIVQACVEKPVRIATRLGI
                     TGEVVHALVPRVAQIVMNTSFRMFPDSEAARGDKGGKPQLSAEAIALQQMMRGIHF"
BASE COUNT          480 a          950 c          875 g          413 t
ORIGIN      
        1 cgacacgctg gccagcccag cctcgtcggg cgccagcgtc tacaacctgc acacgctgca
       61 gctcacggtg tcctacacgc ctgatgtctt cggcggcaac cggcgccagg tggagacatt
      121 gcaggcccag gccgacagcc aacgctggga ggcggaggcg acctatctgg cgctcacctc
      181 caatgtgacc gcggccgccg tgcaggaagc ctcgctgcgc gggcagatcc aggcgacgcg
      241 cgagctgatc gacatccaga ccgagatcct caagcgtttc cagcgccaat accagctggg
      301 ccaggtggcg ctggccgacg tcacccagca ggaggcggca ctggcggccg tgcaggccac
      361 cctgccgccg ctggacaagc agctcgcgct gcagcgcgat ctcatcaagg cccttgcagg
      421 gcagctgccc agcgagcacc tggacgccga gttcacgctc gacagcctca gcctgccggc
      481 cgagctgccg ctgaccctgc cgtccacact ggtcgatcac cgccccgatg tgctcgccgc
      541 tgaggagcaa ctgcacattg ccagcgcaaa catcggcgtg gcgcgagccg cccggctgcc
      601 gagcgtgcaa ttgggcgtga acgcctatgg ctcggcggct tactcgatcc cggacctgtt
      661 caaggcaggt accatcttct ggaccctggc cggcggcgta gcgcagaccg tcttcgacgg
      721 cggcacgctg cgccaccgcg aggcagccgc agccgccgcc tacgaccagg ccgccgcgca
      781 atacagggcc acggtcatca acgcctacca gaatgtcgcc gatgccttgc aggccatcca
      841 ggccgatgcg caggcgctgc gcgctgccga ctacgcccag gacgccgccg atcgcagcct
      901 cgccatcgcg cgccgccagc tcgcgctggg cgacgtgagc cccatgttcg tgatgcaagc
      961 cgagcaggcc gcgctgcagg cgcggttgag cgtggtacag gcccgcgcca atcgcctgtc
     1021 ggactcggtg gcgctgttcc aggctctggg aggcggctgg tggaatcggc aagaatcgct
     1081 gacgcaagca ccgcaataac cgacgcacca tggaaatcca acgccacgag atgggccccc
     1141 gcttcagcga gatgacggtc ttgcgcatcg gcgatgcgaa gctgatctac atcgcgggcc
     1201 aggttgccga aaacacctcg ctcgaagtgg ccggccagac ccgcgaggtg ctgggcgtga
     1261 tcgaccgcct gctggcccag gccggcgcca ccaaggccga catcgtctcg gcacgcatct
     1321 atctcgcctc ggtgggcgac tacgcgacga tgaactcggt ctgggacgac tgggtgccgc
     1381 ccggccacac cccgtctcgt gcaaccatcg gcgccaagct gatcaatccc gagtacaaga
     1441 tcgagatcga agtcaccgcc gctgtgcaag cctgaaacca tggaacaccc tggcaagacc
     1501 cgggccgtgc gcggtttcac actgatggaa ctgatggtcg tgatcgccgt gatcgcgatc
     1561 ctggcgacga tcgcgttgcc caatctccag gggcggctgg tgcgcggcca agtcgtcgag
     1621 gcgatgaagc tggccgacat cgcgaagggc ccggtcgccg cgcgctggag cagcacgaag
     1681 acgctgccgc ccgacaacgc gagcgtcggc ctcccgagcg ccgacaagat cgtcagcaac
     1741 tacgtcagtt cgctcagcgt cgaaggcggc gccatccagg tcaccttcgg caacaacgtc
     1801 aacggcgcca tcaaaggaaa gacactgagc ctgcgccccg ccgtcgtcga agacgccccg
     1861 gtggtgccgg tggcctgggt ctgcggcagt gccccggtgc cggacaagat gaccgcgatg
     1921 ggcgcgaaca agaccgacct gccacctgct ttcctgccgc tcaattgccg ctagatcacg
     1981 acacccgtcg tggactaccg cgaccaccct ccgagagggt gccggcttgc ctctatcaat
     2041 agtcacggca gccggctttg cgcaatcaga aatgaatgcc ccgcatcatc tgctgcagcg
     2101 cgatggcctc ggccgacagc tgcggcttgc cgcccttgtc accccgcgcc gcttcgctgt
     2161 cggggaacat gcggaagctc gtgttcatca cgatctgcgc gacgcgcggc accagcgcat
     2221 gcacaacctc gccggtgata cccaggcggg tggcgatgcg caccggcttc tctacacagg
     2281 cctgcacgat catgtcggcc gcctcctcgg gcgacagcgt cggcacgttg ttgtagatct
     2341 tggtgggcgc gatcatcggc gtgcgcacca gcggcatgtt gatggtcgtg aaggtcacgc
     2401 ccatgtcggc caactcgctg gaggcgcagc gtgtccaggc gtccagcgcc gccttcgacg
     2461 ccacgtaggc cgagaagcgc ggcgcgttgg tcagcacgcc gatcgagctg atgttgacga
     2521 cgtggccctt gcgcttggcg accattcccg gcagcagccc catcgtcacg cgcaggcagc
     2581 cgaagtagtt gagctgcatg gtgcgctcgt agtcgtggaa gcggtcgtag ctcgactcga
     2641 tggcgcgtcg gatcgagcgg ccggcgttgt tgacgaggta gtcgacgccg ccgtacgtcg
     2701 tcgtgagcca ctccacga
//
LOCUS       DASUCY010000318         2013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1356208, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000318 DASUCY010000000
VERSION     DASUCY010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1356208"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..1356
                     /locus_tag="VFV25_06165"
     CDS             157..1356
                     /locus_tag="VFV25_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEY1392939.1"
                     /translation="MARTRKALPAKVDVAHLISTGVLASVCPRRLIDEVLQETGRGSQ
                     RNRLLPAPAVVYYVMALALWREAPLEEVLRVVCEGLQWLGGGQADAVQASKSAISQAR
                     TRLGPEVMQRLAQRVLRPLAEPGAPGAWYRGLRVMALDGSCMDVADERANAEFFGYPT
                     ANRGQSAFPQARVLGLIECGTHAIVAADIAAYARSEKAMAAQLLPGWLHKDMLVLADR
                     GFYSFKLWALARDSGAALLWRVSSTLKLTVQQVLPDGSYLSTVFDSEDSARRHGRTVR
                     VIEYTLESSAVPTQDSYRLVTTLLDSEATPGQELAALYHERWEIEGVFDEFKTHLRAS
                     STVLRSKTPDLVQQELWGLLLAHFAVRQLMAQAAWQRQLDPDRLSFTHAVRVIKRKLP
                     QAAAVPP"
     gene            complement(1661..>2013)
                     /locus_tag="VFV25_06170"
     CDS             complement(1661..>2013)
                     /locus_tag="VFV25_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HEY1392940.1"
                     /translation="VDAGMLTPEQAKVHPQKNQLIAALGIDDELEPHTLSEPEGLADG
                     DAYLLCSDGWWGSLTGQFITAALAVAATPHQWLGLMQERILGLGLPKQDNFSAIGVWV
                     DDPSAATRPMAWSG"
BASE COUNT          337 a          682 c          687 g          307 t
ORIGIN      
        1 gggacgaaaa aacagccgta acccgcgtcg ccgctgggtt acggccggat cagtggctca
       61 aagttcggcc tagccctcgc ctagccccaa tactgttcag atagtccaat aacaggcaca
      121 atgcggttcc gtcgcatttg acggagaacg gaacccatgg cgagaacgcg caaggcgctg
      181 cctgcgaagg tcgatgtggc gcatctgatc agcacgggcg tgctggccag cgtgtgcccg
      241 cgccggctga tcgacgaggt gctgcaggag accgggcgcg gcagccagcg caacaggctg
      301 ttgccggcgc cggcggtcgt gtactacgtg atggcgctgg cgctgtggcg cgaagcgccg
      361 ctggaggagg tgctgcgggt ggtgtgcgag ggtctgcagt ggctcggcgg cggccaagcc
      421 gatgcggtgc aggccagcaa gtcggcgatc tcgcaagcgc gcactcggct cggccccgag
      481 gtgatgcagc gcttggctca gcgcgtgctg cgtccgctgg ccgaacccgg cgcgccgggg
      541 gcttggtacc gcggcttgcg cgtgatggca ctggacggca gttgcatgga cgtagccgac
      601 gagcgcgcca acgccgagtt cttcggctat cccacggcca accgcggtca gagcgccttc
      661 ccgcaggcgc gcgtgctcgg cttgatcgaa tgcggcaccc acgccatcgt ggcggccgac
      721 atcgctgcct acgcacgcag cgagaaggcc atggccgccc agttgctgcc agggtggctg
      781 cacaaggaca tgctggtact ggccgatcga ggcttttaca gcttcaagct gtgggccctc
      841 gcccgcgaca gcggcgccgc gctgctgtgg cgcgtgagtt cgaccctgaa gctgacggtc
      901 cagcaggtcc tgcccgacgg ctcgtacctg agcaccgtgt tcgacagtga agacagcgcc
      961 cgacgccacg gccgcaccgt gcgtgtcatc gagtacacac tggagagctc ggccgtaccc
     1021 acgcaggaca gctaccgctt ggtgaccacg ctgctcgatt ccgaggcgac acccggccag
     1081 gaactggccg cgctgtatca cgagcgctgg gagatcgagg gcgtgttcga cgagttcaag
     1141 acgcacctgc gcgccagcag caccgtgctg cgcagcaaga cgccggatct ggtgcagcag
     1201 gagttgtggg gattgctgct agcgcacttc gccgtgcgtc agctcatggc gcaagcagcc
     1261 tggcagcgcc aactcgaccc agatcgcctg agcttcaccc acgccgtgcg cgtaatcaag
     1321 agaaagttgc cgcaagccgc ggccgttccc ccctgagcac ctcgccagtt ggcgcgacgc
     1381 gctgctcagc gagatcgccc gcggccgctg cgtcagcagc cgtggccgcc acaacccgcg
     1441 cggggtcaag cgcaagatga gcaacttcac cgttcgccac cgcggcgcct cgctccatcg
     1501 ccggcatcga cccacgccta tacttcgtat ctgaacagta ttggccctag gggagcccta
     1561 gggggaacgc cgcctagtcc gcgcgacccg accctgcgcg ccacgcggtc acgtgtgacg
     1621 ccgcgtgaac cgggtcacaa gatgaaagaa ccgcgcggga tcagccgctc caggccatcg
     1681 ggcgcgtggc cgccgacgga tcgtcgaccc acacgccgat cgcgctgaag ttgtcctgct
     1741 tcggcaggcc gaggcccagg atgcgctcct gcatgagccc gagccactgg tgcggcgtgg
     1801 ccgcgacggc gagcgcggcg gtgatgaact ggccggtgag cgagccccac cagccatcgc
     1861 tgcacagcag gtaggcatcg ccatcggcga gcccctcggg ctcgctcagc gtgtgcggct
     1921 ccagctcgtc gtcgatgccc agcgcggcga tgagctggtt cttctgcgga tgcaccttcg
     1981 cctgctcggg cgtgagcatg ccggcgtcga cca
//
LOCUS       DASUCY010000319         2382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_344068, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000319 DASUCY010000000
VERSION     DASUCY010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344068"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="VFV25_06175"
     CDS             <1..796
                     /locus_tag="VFV25_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345588.1"
                     /note="molecular chaperone; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HEY1392941.1"
                     /translation="ANNEDAGQKAKYAEFWREFGAVLKEGVGEDFANRERLAKLLRFA
                     STQADEGTNVMTGLADYVGRMKEGQEAIYVITADSLAAAKNSPQLEIFRKKGIEVLLL
                     TDRVDEWLLSHLYEFEGKPLQSVAKGGVDLGKLQDEAEKQQAEAASEAFKPVLERLKA
                     ALKDKAKDVRATTRLVESPACLVAEEGEVSGHLARLLKQAGQNAPATKPILEVNPEHA
                     LVKRLESSERFDDLAQILFDQALLAEGGQLEDPAAYVRRVNALLAG"
     gene            complement(818..1846)
                     /locus_tag="VFV25_06180"
     CDS             complement(818..1846)
                     /locus_tag="VFV25_06180"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEY1392942.1"
                     /translation="MQEDGHTSALSYAQLTPDAVLDALDSVGLRGDGRLLQLNSYENR
                     VLQVHLEDGRIAVAKFYRPGRWSDEQILEEHGYALELAAAEVPAVAPWPLELDAHASM
                     QPELLGGPPTLARLGALRWSVSPRCGGRAPELEDEEVLAWVGRYIGRLHAVGKRRPFR
                     HRVHWSGADPAQAAFDWLMANDALPLDAAPAWSRMAEQAIAGVRERFAAVPGLRELRL
                     HGDMHIGNLLWTPAGPHFVDLDDACIGPAVQDLWMLLPGERKVSGRAIGCLLEGYTSF
                     CDFDERELELIEALRTVRLIHYSAWLSKRWGDPAFPAAFPWFGSSAYWAEQTTRLRDQ
                     IELMDSPL"
     gene            complement(1895..>2382)
                     /locus_tag="VFV25_06185"
     CDS             complement(1895..>2382)
                     /locus_tag="VFV25_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392943.1"
                     /translation="IETDKPGSRRAAQIKQAVLNACHIRHLKCRADQLPSVAELQLLV
                     PHQGAAARVVPPGMHGLSEASSTLAHTVRTRRAERSALWQDSSFGHDSFFLPDSRVDT
                     AFGHSEFAMSTGPHTLPPAYVARTRAHAPAPRTGNETGGSAETLQIGGVVIDAAHSSA
                     R"
BASE COUNT          395 a          787 c          865 g          335 t
ORIGIN      
        1 ggccaacaac gaagacgccg gccagaaggc caagtacgcc gagttctggc gcgaattcgg
       61 tgccgtgctg aaggagggcg tcggcgaaga cttcgccaac cgcgagcgcc tggccaagct
      121 gctgcgcttc gcgtcgacgc aggccgacga gggcacgaac gtgatgactg ggctggccga
      181 ctacgtgggc cgaatgaagg agggccagga ggccatctac gtcatcaccg ccgacagcct
      241 ggccgcggcc aagaacagtc cgcagctgga gatcttccgc aagaagggca tcgaagtgct
      301 gctgctcacc gaccgcgtcg acgaatggct gctgagccat ctctacgaat tcgaaggcaa
      361 gccactgcaa agcgtcgcca agggcggggt cgatctcggc aagctgcagg acgaggccga
      421 gaagcagcag gccgaggccg cgagcgaggc cttcaagccg gtgctggagc gcctgaaggc
      481 ggcgctcaag gacaaggcca aggacgtgcg cgcgacgacc cggctggtcg aatcaccggc
      541 ctgcctggtg gccgaagaag gcgaggtcag cggccatctg gcgcgcctgc tgaagcaggc
      601 cggccagaac gcgccggcca ccaagcccat cctggaggtg aaccccgagc acgcgctggt
      661 caagcgcctg gagagcagcg agcgcttcga cgacctggcg cagatcctct tcgaccaggc
      721 cctgctggcc gaaggcgggc agctcgagga tccggccgcc tatgtgaggc gggtcaatgc
      781 gctgctggcc ggctgaggtt gcggttcgac gctggcgcta caggggggag tccatcagtt
      841 cgatctggtc acgcaagcgc gtagtttgct cggcccaata ggccgagctg ccgaaccagg
      901 ggaaggcggc gggaaaggcc gggtcgcccc agcgcttcga gagccaggcg ctgtagtgga
      961 tcaggcgcac ggtgcgcagc gcctcgatca attccagctc gcgttcgtcg aagtcgcaaa
     1021 agctcgtata gccctccagc aagcagccga tggcccggcc gctgaccttg cgctcgccgg
     1081 gcagcagcat ccagaggtcc tgtacggccg ggccaatgca ggcatcgtcg agatcgacga
     1141 aatgcggccc ggccggcgtc cacagcagat tgccgatgtg catgtcgcca tgcaggcgca
     1201 gctcgcgcag gccggggacg gcggcgaagc gctcgcgcac cccggcgatc gcctgctcgg
     1261 ccatgcgcga ccaggccggc gccgcgtcga gcggcagcgc gtcgttggcc atcagccaat
     1321 cgaaggccgc ctgcgccgga tcggcgccgc tccaatgcac gcggtgccgg aaggggcggc
     1381 gcttgcccac cgcgtgcagc cgcccgatgt agcggccgac ccaggcgagc acctcctcat
     1441 cctccagctc gggcgcgcgg ccgccgcagc gcgggctcac ggaccagcgc aaggcgccta
     1501 ggcgcgccag cgtgggcggc ccgccgagca gttcgggctg catgctggcg tgagcgtcca
     1561 gttccagggg ccagggtgcg acagcaggca cttcggcggc ggccagctcc agcgcgtagc
     1621 catgttcctc gaggatctgc tcgtcgctcc agcgcccggg ccggtagaac ttggcaacgg
     1681 cgatgcggcc atcctcgaga tgaacctgca gcacgcggtt ctcgtaggaa ttgagctgca
     1741 acagccggcc gtcgccgcgc agccccacgc tgtccagcgc atccagcacg gcgtcgggcg
     1801 tcagctgggc atagctcagc gcgctggtgt gtccatcttc ttgcatgccc gcatagtaag
     1861 cgggcgcgtg gccgggttgc ggtggccccg gcctctagcg tgcgctgctg tgagcggcgt
     1921 cgatgaccac gccgccgatt tgcagcgttt ctgcactgcc gccggtctcg ttgcccgtgc
     1981 gcggcgcggg agcatgggcg cgggtgcgcg cgacgtaggc gggcggcagg gtatgcgggc
     2041 ccgtggacat cgcgaactca ctgtgcccga aggccgtgtc gacgcggctg tccggcagga
     2101 agaacgagtc gtgcccaaag ctcgagtcct gccacagcgc cgagcgctcg gcgcggcgcg
     2161 tgcgcactgt gtgcgcgagc gtggaactcg cctcgctgag tccatgcatg ccgggcggca
     2221 ccacgcgggc cgcggcgcct tgatgaggca cgagcagctg cagttcggcc accgagggca
     2281 gctggtcggc gcggcatttg agatggcgta tgtggcaggc gttgagcacc gcctgcttga
     2341 tctgggcggc gcggcgcgag cccggcttgt cggtctcgat gt
//
LOCUS       DASUCY010000320         2995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_71318, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000320 DASUCY010000000
VERSION     DASUCY010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71318"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="VFV25_06190"
     CDS             complement(<1..935)
                     /locus_tag="VFV25_06190"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HEY1392944.1"
                     /translation="MSKALVIAEKPSVAQDIVRALTPSSGKFDKHDEYFENEHYLVTS
                     AVGHLVEIKAPEEYDVKRGKWSFAHLPVIPPHFDLAPIDKSKGRLNAIVKLAKRKDVS
                     ELINACDAGREGELIFRLIQQYAKAKHPVKRLWLQSMTPQAIRDGFESLRSDAQMHGL
                     ADAARSRSEADWLVGINGTRAMTAFNSRDGGFFLTTVGRVQTPTLSIVVEREEKIRKH
                     VPRDYWEIRASFDAQAGQYEGKWFDPAFKRDENDADKRADRVWDAKTAQAIAEATRGQ
                     PASVTEESKPSTQSSPGLYDLTTLQREANSRFGYSA"
     gene            1038..1532
                     /locus_tag="VFV25_06195"
     CDS             1038..1532
                     /locus_tag="VFV25_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HEY1392945.1"
                     /translation="MTQAPRSSKANGHELPRGRRIQVRLSGVHGKGVFAVAPIAEGET
                     LIEYKGEVITWAEALRRHPHDPKDPNHTFYFHIDDGHVIDAKYGGNAARWINHACAPN
                     CEADEEDGRVFIRALRNIEPGEELFYDYGLVIDERYTPKLKKQYECRCGAKSCRGTML
                     APKR"
     gene            1529..2335
                     /locus_tag="VFV25_06200"
     CDS             1529..2335
                     /locus_tag="VFV25_06200"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEY1392946.1"
                     /translation="MSLPVAPTTMLRWPAEELWEALQPLCPGLSVEVATEVDSTSSRL
                     LERARAGDASPCLLVAERQTAGRGRLGRSWEASPGASLTFSLALPLAPRAWSGLSLVA
                     GVALAEALHERVRLKWPNDLWLVDGAGQGRKLGGILIETAQAAGGPRVAVVGVGLNIR
                     QEAIAGETTSRASLDELESGAQAPATLLRIAAPLLRVLQAFEREGFAPWMARYAARDA
                     LAGRSIRLLQHQGGSSEGQALGVDEQGALLVQTAAGLQTITSAEVSVRPC"
     gene            2329..>2995
                     /locus_tag="VFV25_06205"
     CDS             2329..>2995
                     /locus_tag="VFV25_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392947.1"
                     /translation="MLRVLVLLAALANLVVYAWSQGWLGSISGFGPDADRDPARLARQ
                     VHPEALRILPPGAAASAVAAASAPEAGASSTQASAPESGLACLQAGPYSSAELDQAQG
                     MLKAAALPAGAWAVQSSERPGVWLIYMGKYDSHDALQKKEEELKRLGLRYEELRSSPG
                     LEPGLSLGRFDNRSKADARLAELSKNGLHTARIIIITPAVQQHLLIVMQANAAQQSLL
                     GGLK"
BASE COUNT          455 a         1060 c         1014 g          466 t
ORIGIN      
        1 gccgagtagc cgaagcgcga gttggcctcg cgctgcagcg tggtgaggtc gtacaggccc
       61 gggctgctct gcgtgctggg cttggactcc tcggtgacgc tggccggctg gccgcgcgtg
      121 gcctcggcga tggcctgcgc ggtcttggcg tcccagacgc ggtcggcgcg cttgtcggcg
      181 tcgttctcat ccctcttgaa ggccgggtcg aaccacttgc cctcgtactg gccggcctgg
      241 gcatcgaagc tggcccggat ctcccagtag tcgcgcggca cgtgcttgcg gattttttcc
      301 tcgcgctcga ccacgatgga cagcgttggc gtctgcacgc ggcccacggt ggtgaggaag
      361 aagccgccat cgcgcgagtt gaaggccgtc atcgcgcgcg tgccgttgat gcccaccagc
      421 cagtcggcct cactgcggct gcgcgccgcg tcggccaggc cgtgcatctg cgcatcgctg
      481 cgcagcgact cgaagccatc gcgtatggcc tgcggcgtca tgctctgcag ccacagccgc
      541 ttgaccggat gcttggcctt ggcgtattgc tggatgagac gaaagatcag ctcgccctcg
      601 cgccccgcgt cgcaggcgtt gatgagctcg gaaacgtcct tgcgcttggc gagcttcacg
      661 atcgcgttga gccggccctt gctcttgtcg atgggcgcga ggtcgaaatg cggcgggatc
      721 accggcaggt gcgcgaagct ccacttgccg cgcttgacgt cgtactcctc gggcgccttg
      781 atctccacca gatggcccac cgccgaggtg acgaggtagt gctcgttctc gaagtattcg
      841 tcgtgcttgt cgaacttgcc ggaagacggc gtcagcgcgc gcacgatgtc ctgcgcaacc
      901 gatggctttt ccgcgatgac caatgccttg ctcatatgcc tcgtcttgtt gtcgtgccgg
      961 ctccatacaa tccggctctc gcgcgcacgc gcacacacgc gcgcgcaccc atgaattcac
     1021 tctaaccaaa cctcgcgatg acgcaagcgc cccgatcttc caaggccaac ggccacgagc
     1081 ttccccgcgg gcggcgcatc caggtgcggc tctcgggcgt gcacggcaag ggcgtgttcg
     1141 ccgtggcgcc gatcgccgag ggcgagaccc tgatcgagta caagggcgag gtcatcacct
     1201 gggccgaggc gttgcgccgc cacccgcacg accccaagga cccgaaccac accttctact
     1261 tccacatcga tgacggccat gtgatcgacg cgaagtacgg cggcaacgcg gcgcgctgga
     1321 tcaaccatgc ctgcgccccc aactgcgagg ccgacgagga agatgggcgc gtcttcatcc
     1381 gcgcgctgcg caacatcgag ccgggcgagg agctgttcta cgactacggc ctcgtcatcg
     1441 acgagcgcta cacgcccaag ctgaagaagc agtacgaatg ccgctgcggc gccaagagct
     1501 gccgcggcac gatgctagct ccgaagcgtt gagcctcccc gtcgctccga ccaccatgct
     1561 gcgctggcct gccgaagagc tctgggaggc cttgcagcct ctgtgccccg gcctgagcgt
     1621 cgaggtcgcc acggaggtcg attccaccag cagccggctg ctggaacgcg cgcgggcggg
     1681 cgacgccagt ccctgcctgc tggtggccga gcgccagacc gccggccgcg gccgcctggg
     1741 gcgcagctgg gaggccagtc ccggcgcctc gctgaccttc tcgctcgcgc tgccgctagc
     1801 gcctcgtgca tggtcgggct tgtccctggt ggccggcgtc gccctggccg aggccctgca
     1861 cgagcgcgtg cgactcaaat ggcccaacga cctgtggctg gtcgacggcg caggccaagg
     1921 ccgcaagctg ggcggcatcc tgatcgagac ggcccaggcg gcaggcgggc cccgcgtcgc
     1981 cgtggtcggc gtgggcctga acatcaggca agaggccatc gccggcgaaa ccacgagccg
     2041 cgccagcctg gacgaactgg agtccggggc tcaggcgccg gccacgctgc tgcgcatcgc
     2101 ggcgcccctg ctgagggtgc tgcaggcctt cgagcgcgaa ggcttcgcgc cctggatggc
     2161 gcgatatgcg gcccgcgatg cgctggcagg gcgcagcatt cgcctgctgc agcatcaggg
     2221 cggcagcagc gaggggcagg ccctgggcgt cgacgaacaa ggcgccctgc tcgtgcagac
     2281 cgcagcgggc ctgcagacca tcacaagtgc ggaagtgagc gtgcgcccat gctgagggtt
     2341 ctggtactcc tggccgcgct ggccaatctg gtcgtctatg cctggagcca gggctggctc
     2401 ggcagcatca gcggcttcgg ccccgacgcc gaccgcgatc cggcccgcct ggcgcgccag
     2461 gtccatccgg aggccttgcg catccttccg cccggcgccg ctgcctcggc ggtcgccgcc
     2521 gcttcggccc ccgaggccgg cgcgagctca acccaagcct cggccccgga atcgggcctg
     2581 gcctgcctgc aggccggtcc ctacagcagc gcagaactcg accaggccca aggcatgctc
     2641 aaggccgccg cgctgccggc cggcgcctgg gccgtgcaga gcagcgagcg cccgggcgtc
     2701 tggctcatct acatgggcaa gtacgacagc cacgacgcgc tgcagaagaa ggaagaggag
     2761 ctcaagcgcc tgggcctgcg ctacgaggag ctgcgcagct cgcccgggct ggagcccggc
     2821 ctctcgctcg gccgcttcga caaccgcagc aaggccgatg cccgcctggc ggaattgagc
     2881 aagaacggcc tgcacacggc ccgcatcatc atcatcacgc cggccgtgca gcagcatctg
     2941 ctgatcgtca tgcaggccaa tgccgcccag cagagcctgc tcggcggcct gaagg
//
LOCUS       DASUCY010000321         4796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1307940, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000321 DASUCY010000000
VERSION     DASUCY010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1307940"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..1008)
                     /gene="tatC"
                     /locus_tag="VFV25_06210"
     CDS             complement(217..1008)
                     /gene="tatC"
                     /locus_tag="VFV25_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEY1392948.1"
                     /translation="MTAPNSKDELEGSEQPFVSHLIELRDRLIRALIAVVVVFGVLCA
                     YPGPSGLYDLLAQPLVAHLPQGTRLIATNVISPVLVPLKITLMAAFLVALPVVLYQAW
                     AFVAPGLYSHEKKLVLPLVISSTVLFFIGVAFCYFIVIPAMSRFIQAWAPHSITAAPD
                     IEQYFGFVLTLFIVFGLAFEVPIVVIVLARLGLVSIEQLRKYRGYFIVIAFIVAAIVT
                     PPDVISQLSLAIPMCFLYEIGIWAAKLFIKHTQAPDAEAQDHAAS"
     gene            complement(1021..1497)
                     /gene="tatB"
                     /locus_tag="VFV25_06215"
     CDS             complement(1021..1497)
                     /gene="tatB"
                     /locus_tag="VFV25_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HEY1392949.1"
                     /translation="MIDFGFDKLALIGAVALIVIGPEKLPRVARTVGHLLGKAQRYVA
                     EVKAEVNRSMELEELRKVKTEFEDAARNVEQSVSREIDAARGELEKTWGEAAGSATAA
                     IDGGSIADAPTYKHPGKNWRLKRGAIPQWYKQRHGVRSKAQSGAARVARFRPPRAS"
     gene            complement(1530..1775)
                     /gene="tatA"
                     /locus_tag="VFV25_06220"
     CDS             complement(1530..1775)
                     /gene="tatA"
                     /locus_tag="VFV25_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HEY1392950.1"
                     /translation="MGSFSIWHWLIVLLIVMLIFGTKKLKNIGQDLGSAVKGFKDGMR
                     DGGAASGDAPLPPSQQVTNAGKADDANTVDVEAKSKS"
     gene            complement(1831..2181)
                     /locus_tag="VFV25_06225"
     CDS             complement(1831..2181)
                     /locus_tag="VFV25_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEY1392951.1"
                     /translation="MSADPSCLFCKIVAGQIPSAKVYEDDELIAFKDIHPAAPVHLLV
                     VPKLHVASLELTGPEHQPLLGRMVGLAPRLAHELGASNGFRVVFNNGPDGGQEIYHLH
                     MHILGGPRPWGRMG"
     gene            complement(2185..2547)
                     /locus_tag="VFV25_06230"
     CDS             complement(2185..2547)
                     /locus_tag="VFV25_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392952.1"
                     /translation="MTDYTSAEGGVSADKLASLKQLTMIVYILYALSAFTGITAIAAI
                     IINYLKREDTRGTIYESHFTWQIRTFWWSLLWAVIGFITIIIGVGLLVLAADGIWVLY
                     RIIKGFLNWNDNKPMEVK"
     gene            complement(2540..2896)
                     /locus_tag="VFV25_06235"
     CDS             complement(2540..2896)
                     /locus_tag="VFV25_06235"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEY1392953.1"
                     /translation="MSDSDTLARLAAIIESRKDGDPDKSYVARLFHKGTDAILKKIGE
                     EATETVMAAKDGDAKKLVYEVADLWFHTMIALARFGLKPADVLAELERREGLSGLEEF
                     ALRKAQSREEGERHHD"
     gene            complement(2904..3302)
                     /gene="hisI"
                     /locus_tag="VFV25_06240"
     CDS             complement(2904..3302)
                     /gene="hisI"
                     /locus_tag="VFV25_06240"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEY1392954.1"
                     /translation="MDWLDAIAWDEKGLVPVIAQELGSNDVLMVAWMNREALARTAEL
                     GEAVYWSRSRGRLWHKGEESGNIQKVHEIRLDCDNDVVLLKVTQLGHEPGELGIACHT
                     GRHSCFFQLYKDGAWATVEPVLKDPERIYK"
     gene            complement(3324..>4796)
                     /locus_tag="VFV25_06245"
     CDS             complement(3324..>4796)
                     /locus_tag="VFV25_06245"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEY1392955.1"
                     /translation="DAIEGQRFPQLRYLALSAQGGIHIDFAQLLQDTPLAREQDVRNM
                     LARLALYPSRVDQTIAQLRLGLASGWVTFQSSMQRVPAQIDGQLNDDPRKSPLFEPFT
                     KLPAELTEAQRKQFAAEAEKLLRESTLPALRKLRAFIADEYLPKAPEDGAMSAYPGGA
                     EVYAYRVRQQTTTPLAPKAIHELGLKEVARIRGEMEALMRSTGFQGDFAQFIQFLNTD
                     PRFFYKDGASLLAGYRDIAKRVDPELPKLFAELPRQPYGVRAIPAYQGEGTAEFYTGG
                     AADGSRAGWFNANVVALSHRPTWEMEALFLHEAVPGHHLQTARALELKQLPMFRRNAW
                     YVAYGEGWALYAESLGGQLGLYTDPYSKFGQLRMEIFRAARLVVDTGIHALGWKRQQA
                     IDWMTERTGMARADVEAEVDRYYVWPGQALGYKIGQLKLIELREKARAALGERFDIRR
                     FHMVLLDQGPLPLPVLEQQVDAWIAAERAQAQADRPSSQS"
BASE COUNT          776 a         1537 c         1628 g          855 t
ORIGIN      
        1 gcgcggccgg ccatcacggt gtcgagcagg cccatcagca cgaagccgag ctgggacgcg
       61 atgatggggc cggccaggtg caggagccgg cgcgactctt cgagcacgcg ggctcggcgt
      121 tgattctttt gggtcatgtc ggggcacaaa agcatcacgc ggcgctcgtg gcgccgcgtg
      181 atggtggttc ggggcagtgt agccggccgg gccagctcag gacgccgcgt ggtcttgtgc
      241 ctcggcgtcc ggtgcctggg tgtgcttgat gaagagcttg gcggcccaga tgccgatctc
      301 gtagaggaag cacatcggga tcgcgagcga gagctgcgag atcacgtccg gcggcgtgac
      361 gatggccgcg acgatgaagg cgatgacgat gaaatagccg cggtatttcc tgagctgctc
      421 gatgctcact aatcccaagc gcgctagcac gatgacgacg atgggcacct cgaaggccag
      481 gccgaagacg atgaatagcg tcagcacgaa gccgaagtac tgctcgatgt caggagcggc
      541 cgtgatgctg tgcggcgccc aggcctggat gaagcgcgac atcgccggga tcacgatgaa
      601 atagcagaag gccacgccga tgaagaacag cacggtgctg gagatcacca gcggcagcac
      661 cagcttcttc tcgtggctgt agaggccggg cgcgacgaag gcccaggcct ggtagagcac
      721 gaccggcagc gcgaccagga aagccgccat cagcgtgatc ttcagcggca ccagcaccgg
      781 cgagatcacg ttggtcgcga tcagcctggt tccctggggc agatgcgcca ccaggggctg
      841 ggccagcagg tcgtagaggc cgctcgggcc ggggtaggcg cacagcacgc cgaacaccac
      901 gaccacggcg atgagggcgc gtatcaggcg gtcgcgcagc tcgatcagat ggctgacgaa
      961 gggctgctcg ctgccttcga gctcgtcttt ggaattcggt gccgtcacga gaacttgtct
     1021 tcaggacgca cgcggtggcc ggaagcgggc cacgcgcgcc gcgccggact gggccttgct
     1081 gcgcacacca tggcgctgct tgtaccactg cgggatcgca ccgcgcttga ggcgccagtt
     1141 cttgccaggg tgcttgtagg tgggggcgtc ggcgatgctg ccgccgtcga tcgccgctgt
     1201 cgcgctgccg gcggcctcgc cccaggtctt ctcgagctcg ccacgtgcgg catcgatttc
     1261 ccgggacacg gactgttcga cgttgcgcgc cgcgtcctcg aactcggtct tcaccttgcg
     1321 caactcttcc agctccatcg agcggttgac ctcggccttc acctcggcca cgtagcgctg
     1381 cgccttgccg agcagatgcc ccacggtgcg cgcgacgcgc ggcagcttct ccgggccgat
     1441 gacgatcagg gccaccgcgc cgatgagggc cagcttgtcg aagccgaagt cgatcatgag
     1501 tcagcgccgc cgggcgggac caggcgtcct caggacttgc tcttggcttc gacgtcgacc
     1561 gtattggcgt cgtccgcctt gccggcgttc gtgacctgct gcgaaggcgg cagcggcgca
     1621 tcgccgctgg ccgcgccgcc gtcacgcatg ccgtccttga agcccttgac ggcgctgccc
     1681 agatcctgcc cgatgttctt gagcttcttg gtgccgaaaa tcagcatgac gatcagcagc
     1741 acgatcaacc agtgccagat gctgaaggaa cccatggtgt actcctgaag acgaatccga
     1801 gattttaggt cgtgggtggg gcccgaaagt tcagcccatg cgaccccagg gcctggggcc
     1861 gccgaggatg tgcatgtgca ggtggtagat ctcctggccg ccgtccgggc cattgttgaa
     1921 caccacgcga aagccgttgg atgcccccag ctcatgggcc agccgcggcg ccaggcccac
     1981 catgcggccc agcaacggct ggtgctcggg cccggtgagt tccaggctgg ccacatgcag
     2041 cttgggcacc accagcagat gcacgggcgc agcgggatgg atgtccttga aggcgatcag
     2101 ctcgtcgtct tcatagacct tggcgctggg gatctgcccc gcgacgatct tgcagaacaa
     2161 gcaactgggg tctgcactca taggttactt cacttccatc ggcttgttgt cgttccagtt
     2221 caggaagccc ttgatgatgc ggtaaagcac ccagatgccg tcggcagcca gcacgagtag
     2281 gcccacgccg atgatgattg tgatgaagcc gatgactgcc cagagcaggc tccaccagaa
     2341 ggttcggatc tgccaggtga aatggctctc gtagatggtg ccgcgggtgt cttcgcgctt
     2401 gaggtagttg atgatgatcg ccgcaatcgc cgtgatgccg gtgaaggcgc tcagggcgta
     2461 caggatgtag acgatcatcg tcagctgctt gaggctggcg agcttgtctg cgctgacgcc
     2521 gccctcggcg ctggtgtagt cagtcatgat ggcgttcccc ttcctctctg ctttgcgcct
     2581 tcctcaaggc gaattcctcc aggcccgaca gcccttcgcg ccgctccagc tcggccagca
     2641 cgtcggccgg cttgaggccg aagcgggcca gcgcgatcat ggtgtggaac cacagatcgg
     2701 ccacttcgta gacgagcttc ttcgcgtccc cgtccttggc ggccatcacg gtctcggtgg
     2761 cctcctcgcc gatcttcttc aggatcgcgt ccgtgccctt gtggaagagg cgcgccacat
     2821 agctcttgtc cgggtcgccg tccttgcgcg attcgatgat ggccgccaac cgggccaggg
     2881 tgtcgctgtc actcatcgca ggctcatttg tagatgcgct ccgggtcttt caagaccggc
     2941 tccacggttg cccaagcgcc gtccttgtag agctggaaga agcaggaatg ccggccggtg
     3001 tggcaggcta ttccgagttc accgggctcg tggccgagct gggtcacctt cagcagcacg
     3061 acgtcgttgt cgcaatcgag gcggatctcg tgcactttct ggatattgcc ggactcctcg
     3121 cccttgtgcc acagccgccc gcgcgagcgg ctccagtaca ccgcctcgcc cagctcggcc
     3181 gtgcgcgcca gggcctcgcg gttcatccac gcgaccatca gcacgtcgtt gctgccgagc
     3241 tcctgcgcga tgacgggcac caggcccttt tcatcccagg cgattgcatc cagccagtcc
     3301 atgaggccag tgtagcaatc gcttcatgac tgggatgacg gccggtccgc ttgggcctgg
     3361 gcgcgctcgg cggcgatcca ggcgtccacc tgttgctcca gcaccggcag cggcagcggg
     3421 ccctggtcca gcagcaccat atggaaacgc cggatgtcga agcgctcgcc cagggcggcg
     3481 cgcgccttct cgcgcagctc gatcagcttg agctggccga tcttgtagcc cagggcctgg
     3541 cccggccaga cgtagtagcg gtcgacctcg gcctccacgt cggcacgcgc catgccggtg
     3601 cgctcggtca tccagtcgat tgcttgctgg cgcttccagc ccagggcgtg gatgccggtg
     3661 tcgaccacca ggcgggcggc gcggaagatt tccatgcgca gctggccgaa cttgctgtag
     3721 gggtcggtgt agaggccgag ctggccgccc agcgactcgg cgtagagcgc ccagccctcg
     3781 ccataggcca cataccaggc gttgcggcgg aacatgggca gctgcttgag ctcgagcgcg
     3841 cgcgccgtct gcaaatgatg gccgggcacg gcctcgtgca ggaagagcgc ctccatctcc
     3901 caggtcggcc ggtgcgacaa cgccacaacg ttggcgttga accagccggc gcgcgagccg
     3961 tcggccgcac cgcccgtata gaactccgcc gtcccttcac cctggtaggc ggggatggcc
     4021 cgcacgccgt agggctggcg cggcagctcg gcgaagagct tgggcagttc tgggtcgaca
     4081 cgcttggcga tgtcgcgata gcccgcgagc agggacgcgc cgtccttgta gaagaagcgc
     4141 gggtcggtat tgaggaattg gatgaactgc gcaaagtcac cctggaagcc ggtgctgcgc
     4201 atcagcgcct ccatctcgcc gcggatgcgc gccacttcct tcaatcccag ctcatggatg
     4261 gccttgggcg cgagcggcgt ggtggtctgc tgcctcacgc gataggcgta gacctcggcg
     4321 ccgcccgggt aggcgctcat cgcgccgtcc tcgggggcct tgggcaggta ttcgtcggcg
     4381 atgaaggcgc gcagcttgcg cagcgccggc agggtgcttt cgcgcagcag cttctcggct
     4441 tcggcggcga attgcttgcg ctgggcttcg gtcagctcgg ccggcagctt ggtgaagggt
     4501 tcaaacagcg ggctcttgcg cggatcgtca ttcagctggc cgtcgatctg ggccggcacg
     4561 cgctgcatcg aggactggaa ggtgacccag ccgctggcca gccccaggcg cagctgcgcg
     4621 atggtctggt ccacgcgcga gggatagagc gccagccgcg ccagcatgtt gcgcacatcc
     4681 tgctcgcgtg ccaggggcgt gtcctgcagc agctgggcga agtcgatatg gatgccgccc
     4741 tgggcggaga gcgcgaggta gcgcagttgc gggaagcgct ggccctcgat ggcgtc
//
LOCUS       DASUCY010000322         7414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1516878, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000322 DASUCY010000000
VERSION     DASUCY010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 7414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7414
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1516878"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..638
                     /locus_tag="VFV25_06250"
     CDS             156..638
                     /locus_tag="VFV25_06250"
                     /inference="COORDINATES: protein motif:HMM:NF019237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HEY1392956.1"
                     /translation="MRKWKYTACLAAAMGLACIGAVAADARFVVSADGQEVLDTRTSL
                     VWRRCVEGMTWDGSTCKGKAAKYKFGQAKQKAEVAAPEQGKPWRVPMKDELATLVIQQ
                     KKKPMTDKEAFPNTPASLLWAKRPGFDDNLNGWMIHFGSGKVFGGIGTKGFVRLVRDN
                     "
     gene            790..1341
                     /locus_tag="VFV25_06255"
     CDS             790..1341
                     /locus_tag="VFV25_06255"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY1392957.1"
                     /translation="MVSLRTLTVASNILLLAAAVLAKEPANVVLYLLLIPLNIWRLRE
                     IRRLTRRVEAAAEHRDASGLWLRPYMKTERLKAGQVLFSKGDRADSIFMLVEGELEMA
                     EIGKLVPPGELFGEIACFSPARVRTLTARCITNCVVMSITKEVFDQLYFQEPKLAFRV
                     SNLIAERLSADIARLRKQLDSPH"
     gene            1423..2700
                     /locus_tag="VFV25_06260"
     CDS             1423..2700
                     /locus_tag="VFV25_06260"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HEY1392958.1"
                     /translation="MSQVPVHAHAIGKLSARDVETILGVTRDLAEPLDLHAMLEKVTE
                     AACRVLRAERSSVWLFDASAGELVLKVSRDIGHVRIPLGVGLVGACARERAPINVPDC
                     YADPRFDREIDRRSGFHTRCALTMPLIHTNGSLIGVMQVLNRKDGPFNERDEALAAVL
                     AAQCAVALVRARMTEALVEGEKLRQELELARVVQMSSLPSAMPQLPGYDMHGICLPAE
                     QTGGDTFDLALLAQGVLIVLGDAAGHGLAPALAVTQLQAMLRMAFRLGADLETAFRQT
                     NDQLAATLPDGRFVTAFIGLLDAQTHRVRFLSGGQAPILHFRAEDGTCTSHKATSFPM
                     GAMPLAGLRPAVELVLAPGDMLVLLTDGTYECERPDGTQFGQARVEQLLRELRGAGAA
                     ELARQLVQAVRAFAQGAPQEDDVTIVIVKRCVA"
     gene            2787..3128
                     /locus_tag="VFV25_06265"
     CDS             2787..3128
                     /locus_tag="VFV25_06265"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY1392959.1"
                     /translation="MEIIVTELPDNVRCVRLSGRLDAQGVDRIDTRFTAAVSSSGYPA
                     VVDLSGVTFLASLGIRLLISNARAMKLKGAQLVLFGATELVQNVLDQVALDQIIPIVG
                     TEEQALERLRV"
     gene            3149..3544
                     /locus_tag="VFV25_06270"
     CDS             3149..3544
                     /locus_tag="VFV25_06270"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY1392960.1"
                     /translation="MLTNERTAIEAARQAVLAYLAPWALGPQAAYRVELVLEETLMNI
                     VWHAFPKGGVHPVELTVRVEGDAIELRFENDGIEFDPTTALQPTLPERIEDAHVGGLG
                     LMLVRRCARSLDYRRESGRNRLTVVVAAA"
     gene            3706..4128
                     /locus_tag="VFV25_06275"
     CDS             3706..4128
                     /locus_tag="VFV25_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY1392961.1"
                     /translation="MQEHATQFSHVKPGDTPWRGEGLRDFFLYRDLGIAEATRGKVVA
                     HLVRANHAPEKGTGWHRHEAAFQIVIMIKGWARFMYEDKETLVEAGDCVHQRPGIRHY
                     LFDYSPDMEYLEIVSPADFKTIDVEAPCEVPDPAPWSD"
     gene            complement(4125..4805)
                     /locus_tag="VFV25_06280"
     CDS             complement(4125..4805)
                     /locus_tag="VFV25_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003265692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY1392962.1"
                     /translation="MATSSKTGSKATLSISSKNYSSWSLRGWLLARLAGLDFVEEMRP
                     PDDPDVRAEILLLAPSILVPCLTHNGIKVWDTLAIGEYLNEIKPKAGLLPEDRAARAH
                     CRAICGEMHSGFSALRSALPMNLKAHLPGFKVWAKAQNDIERIVTIWRECLATYGGPY
                     LFGERLTMADAMYAPVVTRFHTYDVKLKHRDCIQYCQRIMALPEMQEWIAAARQEPED
                     IDELDMEF"
     gene            4937..6160
                     /gene="phaZ"
                     /locus_tag="VFV25_06285"
     CDS             4937..6160
                     /gene="phaZ"
                     /locus_tag="VFV25_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HEY1392963.1"
                     /translation="MLYQAFQAYTDLLTPAQMAARACAALMDDPRFGLHGTPDVRRLS
                     AACEVFSRLRMTHHRPSFGIDSVAVDGHDISVEIRTVCGTPFGSLLHFSKAGGRGQGQ
                     SKVLVVAPLSGHFATLLRDTVATMLPEHDIYLTDWHNIRHVPLIEGRFGLDDYVEHVM
                     RFLAELGEGAHMVAVCQPCVPVLAAVALMAEEDHPAQPLSLTLMAGPVDARINPTTVN
                     ELATSRPLDWFERHLINYVPLRYAGALRRVYPGFLQLAAFMSMNMERHAKAFVELFEH
                     LRLGDSDKADTTLRFYDEYFAVNDLSAEFYLETVRLVFQEHALAKGELQYRGRHVDPK
                     AIRRTALLTVEGERDDICAIGQTVAAHDLCTSLRPYMKSHHLQPGVGHYGVFSGRRWQ
                     QQIYPRVRELIQVSL"
     gene            complement(6183..7055)
                     /gene="rlmJ"
                     /locus_tag="VFV25_06290"
     CDS             complement(6183..7055)
                     /gene="rlmJ"
                     /locus_tag="VFV25_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008349200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEY1392964.1"
                     /translation="MFSYRHGFHAGNHADVLKHLVLVQLLQHLLKKDKPLWVIDTHAG
                     GGGYDLTAGYATKNAEFETGIGVLWERDDWPAAVADYLTQVRALNADGALRFYPGSPQ
                     IAAQMLREHDPLRAYELHPTESRLLSAHFDDEAQPHALSRRVSVQASDGFTGLKAALP
                     PPSRRALVLIDPPYEDKADYRKVTTALKDALKRFATGVYAVWYPQVQLTESREFPAQL
                     QRLAQQAGTGWLHASLTVKNPVPGGHGLHGSGIFVFNPAWTLSDALREALPYLLEVLG
                     QDEGADFELRYSKD"
     gene            complement(7078..>7414)
                     /locus_tag="VFV25_06295"
     CDS             complement(7078..>7414)
                     /locus_tag="VFV25_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiopurine S-methyltransferase"
                     /protein_id="HEY1392965.1"
                     /translation="QADVLEWQPEAPLDAVYDQTCLCALHPDHWTAYAAQLHAWLRPG
                     GSLFALFMQARRESADEGRIEGPPYHLDVNAMRALFPEPRWTWPKPPYARVPHPRGWA
                     ELAVHLQRR"
BASE COUNT         1221 a         2414 c         2524 g         1255 t
ORIGIN      
        1 tttgatgacc ggccctagcc cgctcggccg tgagagcagc cgagcgaatc accatcaatg
       61 tggggggttg ccggaggacc gggccctcgg ttaccttttt tgcttgcgca gcgtgcgcgc
      121 cgcaacaatg cggcactatc gccgaggagc ctttcatgag gaaatggaag tacaccgcgt
      181 gtcttgccgc cgcgatgggg ctcgcctgca tcggtgccgt tgccgcggac gcgcggttcg
      241 tcgtttcggc cgacggccag gaggtgctcg acacccgaac cagcctggtg tggcggcgct
      301 gcgtcgaagg catgacctgg gacggcagca cgtgcaaggg caaggccgcc aagtacaaat
      361 tcggccaagc caagcagaag gccgaggtgg ccgcgccgga gcagggtaag ccgtggcgcg
      421 taccgatgaa ggacgaactc gccacgctgg tcatccagca gaagaagaag ccgatgaccg
      481 acaaggaagc tttcccgaac acgccggcat cgctcctgtg ggcaaagcgc cccggcttcg
      541 acgacaactt gaacggctgg atgatccact tcggttcggg caaggtcttt ggcggcatcg
      601 gcaccaaggg gttcgtgcgc cttgtgcgcg acaactaact aatggcgccg ccgcgcgatc
      661 ccgtgggggc gcatgccaga gatcttcgga gacctgctac agccgctgca gacggcgcgc
      721 ggcctgttcg cattcgtggt gagcctagcg ggcgtcgtcc tggtctttgc tgcgtctttc
      781 gtgcgcacga tggtgtcgct gcgcacgctg accgtggcaa gcaacatcct gctgctggcg
      841 gcggccgtgc tcgcgaagga gccggcgaac gtggtgctgt acctcctgct gatcccgctg
      901 aacatctggc gcctgcggga gatccggcgg ctgacgcggc gcgtggaggc ggccgcggag
      961 caccgtgacg cctcggggct gtggctgcgg ccgtacatga agactgagcg tctcaaggcg
     1021 gggcaagtcc tgttcagcaa gggcgacagg gccgattcga tcttcatgct tgtcgagggc
     1081 gagctcgaga tggcagagat cggcaagctt gtgccgcccg gcgagctttt tggcgagatc
     1141 gcgtgtttct cgcccgcgcg cgtgcgcacg cttaccgcgc gctgcatcac gaactgcgtc
     1201 gtgatgtcca tcaccaagga agtgttcgat cagctctatt tccaagaacc caagctcgcg
     1261 ttccgcgtct ccaacctgat cgcggagcga ttgagcgcgg acatcgcgag gctgcgcaag
     1321 cagttggact cgccgcattg agcaaagaat gcgtctgatg ggcaaattgc gcgccgacgg
     1381 tactgaagcc gccacacgcc gcgaagtggc ggcgctccaa ggatgagtca agtcccggtg
     1441 catgcgcacg cgataggcaa gctgtcggcg cgggacgtcg agaccatcct tggggtgacg
     1501 cgcgacctcg ccgagccact cgacctgcac gcgatgctcg agaaggtgac cgaggctgca
     1561 tgtcgcgtgc tgcgtgccga gcgcagctcg gtctggctgt tcgatgcgtc ggcgggcgag
     1621 ctcgtgctaa aggtctcgcg cgacatcggc cacgtgcgca tcccactcgg cgttggcctg
     1681 gtcggcgcct gcgcgcgcga acgcgcgccg atcaacgtgc cggactgcta tgccgacccg
     1741 cgcttcgacc gcgagatcga tcgacgctcg ggctttcaca cccgctgcgc actgacgatg
     1801 ccgctgatcc acacgaacgg gtcgctgatc ggcgtgatgc aggtgctcaa caggaaggac
     1861 ggtccgttca acgagcgcga tgaggcgctg gccgcggtgc tggcggcgca gtgcgcggtc
     1921 gcgctggtgc gcgcgcgcat gaccgaagcc ttggttgaag gcgagaagct gcgccaggag
     1981 ctcgagctgg cgcgcgtggt gcagatgagc agcctgccat cggcgatgcc gcagctgccg
     2041 ggctacgaca tgcacggcat atgcctgccg gcggagcaga ccggcggcga caccttcgac
     2101 ctcgcgctgc tcgcgcaggg cgtgctgatc gtgctgggcg acgccgccgg ccacgggctg
     2161 gcaccggcgc ttgcggtgac gcagctgcag gcaatgctgc gcatggcgtt ccgcctcggg
     2221 gccgacctcg aaaccgcatt ccgccagacc aacgaccagc tcgccgcgac gctgcccgac
     2281 ggacgctttg tgacagcctt cattggtctg ctcgatgcgc agacgcacag ggtgcgcttt
     2341 ctgagcggcg gccaggcccc gatcctgcac ttccgtgccg aagacggcac ctgcacgagt
     2401 cacaaagcga ccagcttccc gatgggcgcg atgccgctgg ccgggctgcg cccggctgtc
     2461 gagctcgtgc tcgcgccggg cgacatgttg gtgctgctga ctgatggcac ctacgaatgc
     2521 gagcgccccg acggcacgca gttcggccaa gcgcgcgtcg agcagctgct gcgcgagctg
     2581 cgaggagcgg gcgcggccga gctggcccgg cagctcgtgc aggcggtgcg cgcatttgcg
     2641 cagggtgctc cgcaagagga tgatgtgacg atcgtgatcg tcaagcgctg cgtggcctga
     2701 gtgtcatgcg gctgtcgccg aactgcctta gcttcgaccc gatcgggtcg tggtcgaacc
     2761 atcgagacaa cccaccagga gccagcatgg aaatcatcgt gaccgaactc cccgacaacg
     2821 tgcgctgcgt gcgcctctcg ggacgcctcg acgcgcaggg cgtagaccgc atcgacacgc
     2881 gcttcacggc ggcggtctca tccagcgggt acccggcggt ggtcgacctg tcgggggtca
     2941 ctttcctcgc ctcgctcggc atccggctgc tgatttcgaa cgcgcgcgcg atgaagctca
     3001 agggtgcgca gctggtgctg ttcggcgcca ccgagctggt acagaacgta ctcgatcagg
     3061 tggcgctcga ccagatcatc cccatcgtcg gcaccgaaga gcaggcgctc gagcggctgc
     3121 gtgtgtagcg gtggaagttc tgcggctcgt gctgaccaac gagcgcaccg cgatcgaggc
     3181 ggcacgtcag gccgtgctgg cgtatctcgc gccatgggcg cttggcccgc aggctgcgta
     3241 ccgcgtcgag ctcgtgctcg aagagacgct gatgaacatc gtctggcacg catttcccaa
     3301 gggcggtgtc caccccgtgg agctcacggt gcgggtcgag ggtgacgcga tcgagctgcg
     3361 cttcgagaac gacggcatcg aattcgatcc aacgacggcg ttgcagccca cgctgcccga
     3421 gcgcatcgaa gacgcgcacg tcggcggcct cggcctgatg ctcgtgcgcc gctgcgctcg
     3481 ctcgctcgac taccggcgtg agagtgggcg caaccggctg acggtggtcg tcgctgccgc
     3541 atagcagcgc acggatgccc gccgtcgcgg ctggggcgcc ggcccgaagc ggtgttcatt
     3601 gccgtccgac ccattccggc acggcttcga tgaagccgcc caggagaccg ggaacgcttc
     3661 gtgcttgaaa tgccgccgtt ccaccggacg ccgagggagg tcctcatgca ggagcacgcc
     3721 acccagttct ctcacgtcaa gccgggcgac acgccctggc gcggcgaggg actcagggac
     3781 ttcttcctct accgcgacct cggcatcgcc gaggccaccc gcggcaaggt cgtcgcgcac
     3841 cttgtcaggg ccaaccatgc acccgaaaaa ggcacgggct ggcaccgtca tgaggcggcg
     3901 ttccagatcg tgatcatgat caagggctgg gcgcgcttca tgtatgagga caaggagacg
     3961 ctggtcgagg ccggcgactg cgtgcatcag cgccccggca tccgccacta cctgttcgac
     4021 tactcgccgg acatggagta cctggagatc gtgagcccgg ccgacttcaa gacgatcgac
     4081 gtcgaagcgc cttgcgaggt gcccgatcca gccccctggt ctgactagaa ctccatgtcg
     4141 agctcgtcga tgtcctcggg ctcctggcgc gccgcggcga tccattcctg catctcgggc
     4201 agcgccatga tgcgctggca gtactgaatg cagtcgcggt gcttgagctt gacgtcgtag
     4261 gtgtgaaagc gcgtcaccac cggcgcatac atcgcatcgg ccatcgtgag gcgttcgccg
     4321 aagagatagg ggccgccgta ggtcgccagg cattcgcgcc agatcgtgac gatgcgctcg
     4381 atgtcgttct gcgccttggc ccacaccttg aagccgggca agtgcgcctt caggttcatc
     4441 ggcagcgccg agcgcaaggc cgaaaacccc gaatgcatct cgccgcagat ggcgcggcag
     4501 tgcgcgcggg cggcgcggtc ttccgggagc agcccggcct tgggcttgat ctcgttgaga
     4561 tattcgccga tcgccagcgt gtcccagacc ttgatgccgt tgtgcgtgag gcaaggcacc
     4621 aggatagacg gcgccagcag caggatctcg gcccgcacgt cgggatcgtc cggcgggcgc
     4681 atttcctcga cgaagtcgag tccggccagc ctggccagca gccacccccg cagcgaccag
     4741 gacgagtagt tcttgctgct gatggaaaga gtggctttcg agccggtctt cgaactggtg
     4801 gccatgggcc tgctcctcgt cgcggcgccc tgcgtacgct cgccacgcaa ccgccatgcc
     4861 agggcagctc gcattggccc gcaatttgcc tagccgtgca agaggccggc agctgccggc
     4921 tcaggatgag gcgagcgtgc tctaccaagc ctttcaagcg tatacggatc tgctgacgcc
     4981 ggcgcagatg gcggcacgcg cttgcgccgc gctgatggac gatccccgct tcgggctgca
     5041 tgggacccct gatgttcgcc gcttgtcggc cgcctgcgaa gtgttctccc ggctgcgcat
     5101 gacgcaccac cggccgtcgt tcggcatcga cagcgtggcg gtcgacggcc acgacatttc
     5161 cgtcgagatc cgtacggtgt gcggcacccc cttcggctct ctgctgcatt tcagcaaggc
     5221 cggtggccgc ggtcagggcc agtccaaggt gctggtcgtg gccccgctgt ccgggcattt
     5281 tgcgaccctg ctgcgcgata ccgtggccac catgctgcct gagcacgaca tctacctcac
     5341 cgactggcac aacattcgcc atgtgccgtt gatcgagggc cggttcgggc tcgacgacta
     5401 tgtggagcac gtgatgcgct tccttgccga actgggcgaa ggcgcccaca tggtcgcggt
     5461 ctgtcagccc tgcgtgccgg tgttggctgc cgtggccctg atggcggagg aagaccaccc
     5521 cgctcagccg ttgagcctga cgctgatggc aggcccggtg gatgcgcgca tcaacccgac
     5581 cacggtcaac gagctggcga ccagccggcc gctcgactgg ttcgagcgtc acctgatcaa
     5641 ttacgtgccc ttgcgctatg ccggcgcact gcggcgcgtc taccctggct tcctgcaact
     5701 ggccgccttc atgagcatga acatggagcg gcatgccaag gccttcgtcg agctcttcga
     5761 gcatctgagg ctgggggact ccgacaaggc cgacacaacg ctgcgcttct acgacgaata
     5821 cttcgccgtc aatgacctgt cggccgagtt ctacctggaa accgtgcgcc tcgtattcca
     5881 ggagcacgcg ctggccaaag gcgagctgca gtaccgcggg cgccacgtcg acccgaaggc
     5941 gatacgccgc acggccctgc tcaccgtcga gggcgagcgc gacgacatct gcgccatcgg
     6001 acagaccgtg gcagcgcacg acttgtgcac cagtctacgt ccctacatga aaagccacca
     6061 cctacagcca ggcgtaggcc attacggcgt gttcagcggc cggcgctggc agcagcagat
     6121 ctatccgcgc gtgcgcgagc tgattcaggt cagtctctag acccgcgctg aagggcccag
     6181 cctcaatcct tcgaatatct caactcaaaa tccgccccct cgtcctgccc caacacctcc
     6241 agcagataag gcagcgcttc gcgcaaagca tcactcaggg tccacgcggg gttgaagacg
     6301 aagatgccgc tgccgtgcag gccgtggccg ccgggtacgg ggttcttcac ggtgagcgag
     6361 gcgtgcagcc acccggtgcc ggcctgctgc gccagccgct gcaactgggc cgggaattcg
     6421 cgtgattcgg tcagctgcac ctgcggatac cacacggcgt agacgccggt ggcgaagcgc
     6481 ttgagtgcgt ccttcagcgc ggtggtgacc ttgcggtagt cggccttgtc ttcgtagggc
     6541 gggtcgatca gtaccagcgc gcggcgcgag ggcgggggca gtgcggcctt gaggccggtg
     6601 aagccgtcgc tggcctgcac cgaaacgcgg cgcgagaggg catgcggctg tgcttcatca
     6661 tcgaaatggg cgctcagcaa gcggctctcg gtggggtgca gttcgtaggc gcgcaggggg
     6721 tcgtgctcgc gcagcatctg cgcggcgatt tgcggcgagc cgggatagaa gcgcagggcg
     6781 ccgtcggcat tgagcgcgcg cacctgggtc aggtagtccg ccacggccgc gggccagtcg
     6841 tcgcgttccc acagcacgcc gatgccggtc tcgaactcgg cgttcttggt cgcgtagccg
     6901 gcggtgaggt cgtagccacc gcctcccgcg tgggtgtcga tgacccacag cggcttgtct
     6961 ttcttcagca ggtgctgcag cagctgcacc agcaccagat gcttgagcac gtcggcatgg
     7021 ttgccggcgt ggaaaccgtg gcggtagctg aacatggttg ggcgctcgtt gaagggttca
     7081 gcgccgctgc agatgcacag ccagctcggc ccagcctcgc gggtggggca cgcgcgcata
     7141 gggcggcttg ggccaagtcc agcgcggctc gggaaagagg gcgcgcatcg cattgacgtc
     7201 gaggtgatag ggcggcccct cgatgcggcc ttcgtcggcg ctttcgcgcc gcgcctgcat
     7261 gaacaaggcg aacagcgaac cgcccgggcg cagccaggcg tgcagctgcg cggcataggc
     7321 cgtccagtgg tcggggtgca gcgcgcacaa gcaggtctgg tcgtagacgg catccagcgg
     7381 cgcttcgggc tgccactcga gcacgtcggc ctgc
//
LOCUS       DASUCY010000323         3294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_440678, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000323 DASUCY010000000
VERSION     DASUCY010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440678"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="VFV25_06300"
     CDS             <1..633
                     /locus_tag="VFV25_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HEY1392966.1"
                     /translation="PNNTYSGGASKIFEKFQYNDKRTADTNNQNSTTPFYNQSCGLDA
                     NFNCTHVQVLPTLNLNRVYVLTSGNTCSASESVVNSLRGVNVTVNLVGTTTCGKPYGF
                     TAHDNCGVSYFPIEFKGTNNVGFGDYSDGFTPSCTAADDLSHALGDTAEGKLAAALYM
                     RAYNGACTPIASASGTTKGIQSVRDAIVADGLLLRSNPTLENRIFLPLKR"
     gene            758..1300
                     /locus_tag="VFV25_06305"
     CDS             758..1300
                     /locus_tag="VFV25_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY1392967.1"
                     /translation="MTRILILPGLFNSGPEHWQSRWEVLHGDRRVEQDDWDWPKRGDW
                     MARLDEVLLSSEQPALLAAHSLGCQLVAAWAAHSRHTGRVRGALLVAPPDTEGEDLPP
                     QLACWRPIPLQHLPFAATAVVSSNDPFCALERASLMSQAWGANMVLAGPRGHLNGESG
                     LGDWPDGRALLQALNGAETV"
     gene            1344..2261
                     /locus_tag="VFV25_06310"
     CDS             1344..2261
                     /locus_tag="VFV25_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEY1392968.1"
                     /translation="MVTKKPKGLGLGLEALLGPKVVDAPAADGQPTVLKLDQLQPGKY
                     QPRTRMDEGSLFELAESIKSQGVMQPILVRPLDGANGGQDGSGKQRYEIIAGERRSRA
                     ARLAGLDEVPVLVKDVPDEAAAVMALIENIQRENLNPLEEAQGLKRLTDEFGLTHEQA
                     AQAVGRSRSAASNLLRLLNLAEPVQQMLMSNDIDMGHARALLALDRAHQITAGHEVVA
                     KKLSVREAERLVARALAGRQTPLMRIKDEKPRDLLRVEEELADLLTAAVDIRIKKRTK
                     RGDQGELAIAFGSLEELNGLIDKLRATQG"
     gene            2887..>3294
                     /locus_tag="VFV25_06315"
     CDS             2887..>3294
                     /locus_tag="VFV25_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HEY1392969.1"
                     /translation="MDVISNFAARYERTREEEFTLQEYLDICKRDPLAYATAAERMLK
                     AIGDPEMVDTRNDPRMSRIFANKLIKRYPAFAEFYGLEDSIEQVVAYFRHGAQGLEEK
                     KQILYLLGPVGGGKSSIAERLKQLMEQVPFYALK"
BASE COUNT          627 a         1120 c         1038 g          509 t
ORIGIN      
        1 ccgaacaaca cctatagcgg cggcgcttcc aagatcttcg agaagttcca gtacaacgac
       61 aagcgcacgg ccgacaccaa caaccagaac tcgaccacgc ccttctataa ccagtcctgc
      121 ggtctggacg ccaatttcaa ctgcacacat gtgcaagtcc tgccgacgct caacctcaac
      181 cgggtctatg tcctgaccag cggcaacacc tgttcggcca gcgagtcggt cgtcaatagc
      241 ctgcgcggcg tcaatgtcac ggtcaacctg gtcggtacca cgacctgcgg caagccctat
      301 ggcttcacag cccatgacaa ctgcggcgtg tcctatttcc cgatcgaatt caagggcacc
      361 aacaacgtcg gcttcggcga ctactccgac ggcttcacgc ccagctgcac ggcggcggac
      421 gacctcagcc atgcgctggg cgacacggcc gaaggcaagc tcgctgcggc tctttacatg
      481 cgcgcctaca acggtgcttg cacccccatc gcaagcgcga gtggtaccac caagggcatc
      541 caatcggtca gggacgcgat cgtcgccgac gggctgctgt tgcgtagcaa cccgacactg
      601 gagaaccgca tcttcctgcc gctcaagcgc tgagcaaggc cgcggcaggc ccggctcgcc
      661 gcggggagaa acaagcggcg cggtccgaaa ggccgcgccg ctcttttttc tgtgccgcag
      721 ctaggctatc cgacaatccc ggcccatgag cgcttcgatg acacgcatcc ttatccttcc
      781 gggcctcttc aactctgggc ccgagcactg gcaaagccgc tgggaggttt tgcacggcga
      841 ccgccgcgtc gagcaggacg actgggactg gcccaagcgc ggcgactgga tggcaaggct
      901 ggacgaggtg ctgctgagca gcgagcagcc cgccctgctg gccgcccaca gcctgggctg
      961 ccagctcgtc gccgcctggg ccgcccattc gcggcatacc ggccgcgtgc gcggggcctt
     1021 gctggtggcg ccgcccgaca ccgagggcga ggacctgccg ccccagctcg cctgctggcg
     1081 gccgatcccg ctgcaacacc tgcctttcgc cgccaccgcc gtggtgagct ccaacgatcc
     1141 cttttgcgcg ctggagcgcg ccagcctgat gagccaggcc tggggcgcga atatggtgct
     1201 ggccggcccg cgcgggcacc tgaatggtga atccggcctg ggcgactggc ccgacggccg
     1261 cgcgctgctg caggccctga acggcgccga aaccgtttga gtattccgtc ggccctcgtg
     1321 ggccggcagg cgacaatcgc accatggtga ccaagaaacc caagggcctg ggcctgggcc
     1381 tcgaggcctt gctcgggccc aaagtcgtcg acgcgcccgc agccgacggc cagcccaccg
     1441 ttctcaagct cgaccagctg cagcccggca agtaccagcc gcgcacccgg atggacgaag
     1501 gctcgctctt cgagctggcc gaaagcatca agtcgcaggg cgtgatgcag cccatcctcg
     1561 tgcgcccgct cgatggcgcg aatggcggcc aggacggcag cggcaagcag cgctacgaga
     1621 tcatcgccgg cgagcggcgc tcgcgcgccg ccaggctggc cgggctggat gaagtgccgg
     1681 tgctggtcaa ggatgtgccc gacgaagcgg ccgccgtgat ggcgctgatc gagaacatcc
     1741 agcgcgagaa tctcaatccg ctcgaagagg cgcaaggcct caagcgcctg acggatgaat
     1801 tcgggctgac ccatgagcag gccgcgcagg cggtcgggcg gtcgcgcagc gccgccagca
     1861 atctgctgcg cctgctcaac ctggccgagc cggtgcagca gatgctgatg tccaacgaca
     1921 tcgacatggg ccatgcgcgc gcgctgctgg cgctggaccg cgcccaccag atcacggccg
     1981 gccacgaggt cgtggccaag aagctctcgg tgcgcgaggc cgagaggctg gtcgcgcgcg
     2041 ccctggccgg ccggcaaacg cccttgatgc gcatcaagga cgagaagccg cgcgacctgc
     2101 tgcgtgtcga agaggagctg gccgatctgc tcaccgctgc ggtggacatc cgcatcaaga
     2161 agcgcaccaa gcgcggcgat cagggcgagc tggcgatcgc cttcggctcg ctggaggagc
     2221 tcaacggcct gatcgacaag ctccgagcga cgcaaggctg acgccgccag cgcttcatcg
     2281 acaacgcggc ccggctgcgg gatttcgtct tcaagctcac gactcgtcac gaacccgagc
     2341 gcaaccagcc atgccggctt ggcgacgcgc agtcagcctc gaaaagagac atagctgtct
     2401 cacctggatg cagacgccag ccctggtttt ctcaatggta tgagcgatag caatccacaa
     2461 gggatcgttt caggacattt cggcttgacc cagagggcca ttcctcgcgg gctcgcgaat
     2521 gcgcctcctt ctcgcgcacg tgcctgtgcg ggcgcagcgt gcgtgccggt gcgagcacgc
     2581 acgagcacct catcgcccgt tgacggcgta tgcacgtagg aactcacgga aacaccaggg
     2641 ctcccttgaa aaccggtggc gcgcgctgcc ggaaaggggc atgctccttg cacgcgactc
     2701 atggagctca tggcaagcgg cgaatcggca cttctcgcca cctgcatatg agtgggtgaa
     2761 caaacattgt tgacgatctt tgacgatcat tgagcatcag gcagcgctgc acggtggtcc
     2821 cggaccccgg gccacatggc gacgccgcct ccacaagctt cccgctggga atggaggtaa
     2881 gaacacatgg acgtaatcag caacttcgcc gcacgctacg agcgcacgcg tgaggaagaa
     2941 ttcacgctgc aggaatatct cgacatctgc aagcgcgacc cgctggccta tgccaccgcg
     3001 gccgagcgca tgctcaaggc catcggcgat cccgagatgg tggacacgcg caacgaccca
     3061 cgcatgtcgc gcatcttcgc caacaagctc atcaagcgtt accccgcctt tgccgagttc
     3121 tacggcctgg aagactcgat cgagcaggtc gtggcctatt tccgccatgg cgcccagggg
     3181 ctggaggaaa agaagcagat cctctatctg ctgggcccgg tgggcggtgg caagagctcg
     3241 atcgccgagc ggctcaagca gctgatggag caggtgccct tctatgcgct caag
//
LOCUS       DASUCY010000324        12689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1597182, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000324 DASUCY010000000
VERSION     DASUCY010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 12689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12689
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1597182"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VFV25_06320"
     CDS             <1..364
                     /locus_tag="VFV25_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEY1392970.1"
                     /translation="SVRVDGTAIDVPLTEARAERALLGVRPEQVRLLAPEAGLRAQVR
                     HAEYFGSHWIVELHTAVGALKALVDKAAKPTEGEHVGLAFDTQRIVLFDATSEQLLAS
                     ATTSTHQPCMHHVQHLPQ"
     gene            342..1364
                     /locus_tag="VFV25_06325"
     CDS             342..1364
                     /locus_tag="VFV25_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY1392971.1"
                     /translation="MSSISLSSVSKRFGSVAAVDDLRLDIADGEFFVLLGPSGAGKTT
                     TLRLVAGLETPDAGSVCMNGADITAVPPAQRDCAFVFQQYSLYPHLTVFDNIAFPLRS
                     PLRRVDETQIYRRVQRVAEMLHIESKLAAKATALSGGEMQRVAIGRALVREPRVYLMD
                     EPLSSLDAKLREELRVELKRLQRQSGATVLYVTHDQVEATTLADRIAILEQGRIQQVG
                     TPLQIYEDPQTLHVAQRLGQPAINVLPTEWFGGSAHPHAAQVAIRPEDVVVGVGDVLP
                     YFDVIECSLVKHLVVAGRGGVEVRARTMLDQALSPGSAVRMSFPRERCLYFDASGQRL
                     TTLETA"
     gene            1361..1999
                     /gene="dhaL"
                     /locus_tag="VFV25_06330"
     CDS             1361..1999
                     /gene="dhaL"
                     /locus_tag="VFV25_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HEY1392972.1"
                     /translation="MNTPLIIKSLAAVQRAILANEAHIESLDRAIGDGDHFINVRRGC
                     DVLVAMSPGLHDAPPAVALQKIGLKLLGTIGGASGPLISSFFIAMGKALDGIAEPDRQ
                     QFAVAFAAGVEAIKTRGKADVGEKTMLDVLIPASRLLTRLTDERASLAALCEQMTEEA
                     HRNMLATKDMIATKGRAYFLGERALGHIDPGCKTCEVAIAAVCNTVLAKEPA"
     gene            1996..2979
                     /gene="dhaK"
                     /locus_tag="VFV25_06335"
     CDS             1996..2979
                     /gene="dhaK"
                     /locus_tag="VFV25_06335"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEY1392973.1"
                     /translation="MKKFINDIDSVMRESLGGLLAAHPDLLRVEFEPTFVARATKALN
                     KVALISGGGSGHEPLHAGFVGGGMLDAACPGQVFTSPTPDQMLAAAQSVHADRGVLFI
                     VKNYAGDSMNFQMAAEMLEIENATVLVNDDVAVENSTHTAGRRGVAGTVMVEKMVGAA
                     AEAGASLQECKAMGDRVNAATASMGVAFTSCTVPAAGRATFEIGDDEMEVGVGIHGEP
                     GRRREKLKPAAAIVEELLSSILADLKPQRGREALLHVNGFGGTPQMELYLLYHCAAEQ
                     LRKLGLQPVRSLVGNYTTSLEMAGASLTVTLLDDEMKSLWDAPVHTPALRW"
     gene            2984..4132
                     /locus_tag="VFV25_06340"
     CDS             2984..4132
                     /locus_tag="VFV25_06340"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY1392974.1"
                     /translation="MKRRAVLCWAGSAVLPPLAWPLNAAAIATERSPIRLGQSAPITG
                     PLARVGTAFRDTARAVFAEATAAGGVAGRAIELVTLDDEDRPERTAVNVKLLASEYNV
                     VGLFGFVGAGAHRAGARGAADEGLPYIAAVSGSQELRSGALPWIFNLRAGHLDEIEYI
                     VRHTQQVGITRVSLVSEYNSQGWELRDALITSFEARGSQVASISSIDHEGSRYSLPGA
                     VSSVLAGAPQAIVLGADYVASARFVTAVREAGFTGLFYTLSTVGGEAMTEQLGPRAVG
                     LSVTQVVPFPWTASLPVGRSFQSFCTRHKLEPSFASMEAWLAANLLVDALRRARDATP
                     AGLVDALQALPPRDFGGYVAAFYSSARSRRTPPQVDLTVFSRAGKFIK"
     gene            4138..4881
                     /gene="tpiA"
                     /locus_tag="VFV25_06345"
     CDS             4138..4881
                     /gene="tpiA"
                     /locus_tag="VFV25_06345"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011401953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEY1392975.1"
                     /translation="MKLVIGNWKMNGSLAALREFAQAAQGSWPACEAALCVPFPLLAA
                     AQSALQATPLRWGAQDCAAHGEGAYTGDVSAGMVAGFGTRYVIVGHSERRIRHGETDA
                     EVAEKARCVIAAGMTPILCVGETEAERDGNQTPAVLRRQLLQALSVLGRDFVRAIVAY
                     EPVWAIGRGDAATPGLIADTHGLIEQTLMIHAGLSAQAVRLLYGGSVNARNASAILST
                     PLVAGALVGGASLRPAEFVAICRADEARA"
     gene            5445..7793
                     /locus_tag="VFV25_06350"
     CDS             5445..7793
                     /locus_tag="VFV25_06350"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY1392976.1"
                     /translation="MAMCIRVAQDDELQSRTRARCDDPAAAPGREALSPLLEVAVGVA
                     DAKGAALFLAADEQPILVGTIPLPGWPGIRRMSECVGAAQGGTGPDVELEDCGGLIRV
                     TLRETPQAPAQRLVVWGVPPLAGTQRAKLLGAARALLAYLRTERQVRAAALNARRVQD
                     VAYASGEWLWETDAEHRYTWVMRDPSRGPDGASEPRVGSEIAQAYVVDCFGMPEVPET
                     TLREVLAECRPIVRLVVGESDGTRIRFVSRSAVPIVGTDGGFLGYRGSARDVTDSVQA
                     KAELWMRDEQLRRSAQALPGAVLQVGCGSWLSARIEDHSGRLAELLGLPGALRSYPLR
                     GALRKLPAAERARLHEVARSGVPTLFLRLRTLCMPPAASWIELHATANRKAGGATSWS
                     VFISDATQRVRDEQALRAHEQRWGLAAGAAGLGLIEIDLVAGCATFDERSVALIGTSN
                     CAAGRQTQLHDLLERLDAEGRERLVERLADPLADASALSLELDGGRHLNMLVCPPEPS
                     NPPGGRLTVICRDTTSEHQIGRLRLAKEAAEAASRAKSMLVSKVGHELRTPLNAIVGL
                     SQLIRLKRDGLQETASVDGWIEQIASTGRHMAGVIETLMQLGQSGAGRLQIAEQSFDV
                     REPMEEARRILEQEAAQRGVAIEADAALPVKVLADRRAVRQVFLNLLSNAIKYNREGG
                     GVRVVVQDGTRVRITVHDTGPGLSAEQRLRLFQPFERLGAESSGVEGYGLGLLVCKEL
                     LAAMDGTIDAESVPGAGCAFIVTLPGVAECAASPTKAESDVG"
     gene            complement(8219..8806)
                     /locus_tag="VFV25_06355"
     CDS             complement(8219..8806)
                     /locus_tag="VFV25_06355"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HEY1392977.1"
                     /translation="MMKLAVHALPDAAGAWPRLSATQPTVLALKLPRGTRRERAREQV
                     RTALRGLLGAHFGIAAEAVALSGTPGQPIRLDHPEAPIEISISHAEGCSLLALWPQGE
                     VGVDLMLPIPLPEMQNLASQYLGPEVAQTLAAVAVPKREQAFAAAWCRHEAALKCLGE
                     PLDEWSEAMAARLLRCRLAMLDLPAPWVGATAWLT"
     gene            complement(8812..>12689)
                     /locus_tag="VFV25_06360"
     CDS             complement(8812..>12689)
                     /locus_tag="VFV25_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Pls/PosA family non-ribosomal peptide
                     synthetase"
                     /protein_id="HEY1392978.1"
                     /translation="QRSLSYAELDELASVVASNLIAAGVGPGSIVGLWLPRCLDLLVA
                     QAGIAKAGAAWLPFDADTPVERIAVCLDDAKSACLLSCEAWLPRLDALPQPVLTAEAL
                     SLRPSGALQRRAAVQANDPAYVIYTSGSTGRPKGIVISQGSICHFLRSENERLGVRQS
                     DRVYQGFSVAFDMSFEEIWISYLVGASLWIAPREIASDPEALPAALIEQQITVLHAVP
                     TLLALFAQDVPSLRLINLGGEMCPQALVARWARPGRQVFNSYGPTEATVSASMAELHA
                     GEPVTIGEPLPNYVLLVVAPEVVDGQGLKLLPRGEVGELCISGPGVAAGYLGRPELTA
                     EKFLANPWANGDHDARLYRTGDLARIDAHGRVECLGRTDDQVKLRGFRVELGEIEAQL
                     ARQPGVGTVAVVLHNSDGLEQLVAFLVPRDGAAIDAAALRTALAAVLPPYMVPSRYEG
                     LSEMPRLPSGKIDRKDLKARRLSIAPAAGESDRPEGAAEELLFAALQQLFPGLPLRRD
                     ADFFSDLGGHSLFAARLASAIRADAHYAQFSVRDIYQQRRLGAIAQAMGEAELRRGQA
                     AAKPRAFTTTPALRRWLCGAAQALAVPPLVALRMAQWLAPFFTYHFFTGGPNDSIAFA
                     VAISLAAFLLATMLAFVVTIAGKWLVLGRARPGRYPLWGLTYFRWWLSDRLIEAAPTH
                     MLPGSSLFPMYLRALGARVGRDAQIGSLTLRAPDLLSIGDGASLGTAVNLENARVEGG
                     ELILGRIEIAANAYVGSFSVLEGNTTLGEYAHLDGISALADGQQIPARRIWSGSPARE
                     TGVFDPASRPPRPAVEAWRPALEWFYFSAGSLLIAALFFMPVFPAFMLIDWLDDADRF
                     PWLQGNVVWIQLLKYFVLSLPASAVLILLTALISAALRWTFLPRLGEGSWPVRSRTYY
                     EKWLVNQIQETSLQVLHGVYATVYAPFWYRLLGAKVGRHAEISTALGVVPDMLTLGDE
                     TFIADNVLLGDEEIDGGWMTMQPTVVSRRSFVGNGAYVPDGSVLPENLLIGVHTRVPE
                     NAQMREGDTWLGSPPINLPAREQVAGYPDALTFRPSPLRRLGRGLVEALRIVAPHAIV
                     IATGYTIVLDLMPAAGEEDWGQVVIGLTLAGLLYGLGSLAFVALLKWLLIGRYSRHAV
                     PMWTRFVWFSEAVTNLYEGIAVPNFVRYLRGTPWLPWALRLLGTRIGRGVYLDTTDIT
                     EFDCVRVGDYSELNALSCPQTHLFEDRVMKIDVVDIGARVTMGARSTVLYGASVGDDA
                     WLGPLTLAMKGEQLPAGSSWRGCPAAPAA"
BASE COUNT         2023 a         4194 c         4499 g         1973 t
ORIGIN      
        1 gagcgtgcgc gtggacggca cggccatcga cgtgcccctc accgaagcgc gcgccgaacg
       61 tgcgctgctg ggcgtgcggc cggagcaagt acgcctgctt gcccccgaag ccggtctgcg
      121 cgcgcaagtg aggcacgccg aatacttcgg ctcgcactgg atcgtcgagc tccacaccgc
      181 cgtcggcgcg ctgaaggcgc tcgtggacaa ggccgcgaag ccgactgaag gcgagcacgt
      241 cggcctcgcc tttgacacgc agcgcatcgt gctcttcgat gcgaccagtg agcagctgct
      301 agccagcgcc accacctcca cccatcaacc gtgcatgcac catgtccagc atctccctca
      361 gtagtgtgag caagcgcttc gggtcggtgg cggcagtcga cgacctgcgg ctcgatatcg
      421 ccgacggcga gttcttcgtg ctgctgggcc cgagcggtgc cggaaagacg accacgctgc
      481 gcctggttgc ggggttggag acgcccgatg ccggcagcgt gtgcatgaac ggcgccgaca
      541 tcactgcggt gccgcccgcg cagcgcgact gtgccttcgt gttccagcag tactcgctgt
      601 acccgcatct cacggtgttc gacaacatcg ccttcccgct gcgctcgccg ctgcgtcgcg
      661 tcgacgagac ccagatctac aggcgcgtgc aacgcgtggc cgagatgctg cacatcgaaa
      721 gcaagctggc tgccaaggcc accgcgctct cgggcggcga gatgcagcgt gtggcgatcg
      781 gccgcgcgct ggtgcgcgag ccgcgcgtgt acctgatgga cgaaccgctg tcctcgctcg
      841 acgccaagtt gcgagaggaa ttgcgcgtgg agctgaagcg cctgcagcgc caaagcggcg
      901 ccacggtgct ctatgtgacg cacgaccagg tcgaggccac gacgctggcc gaccgcatcg
      961 ccatcctcga gcaggggcgc attcagcagg tcggcacgcc gctgcagatc tacgaagacc
     1021 cgcagactct gcacgtcgcg cagcgcctgg gtcagcccgc gatcaacgtg ctgccgaccg
     1081 agtggttcgg cggcagcgcg catccgcacg cggcgcaggt cgccatccgt cctgaagacg
     1141 tggtggtggg cgtcggtgac gtgcttccct atttcgacgt catcgagtgc tcgctggtca
     1201 agcacctggt ggtggccggg cgcggcggcg tggaagtgcg cgcccgcacg atgctggacc
     1261 aggccctttc ccccggatcg gcggtgcgga tgtcgttccc acgcgagcgc tgcctgtatt
     1321 tcgacgcatc gggccagcgc ctgacgaccc tggagactgc atgaacactc ccttgatcat
     1381 caaatcgctc gctgcggtgc agcgggcgat cctggccaac gaggcgcaca tcgaatcgct
     1441 cgacagggcc atcggcgacg gcgaccactt catcaacgtg cgccgcggct gcgacgtgtt
     1501 ggtggcgatg agtcccggcc tgcatgacgc gccacctgcg gtggcgctgc agaagatcgg
     1561 cctgaagctg ctcggcacga tcggaggcgc ctcgggtcca ctgatctcca gctttttcat
     1621 cgcgatgggc aaggcgctgg atggcatcgc cgagccggac cgccagcagt tcgccgtggc
     1681 gttcgctgcc ggcgtggagg cgatcaagac gcgcggcaag gccgatgtgg gcgaaaagac
     1741 gatgctcgac gtgctgatcc cggcgtcgcg cctgctcacg cgcctgacgg acgagagggc
     1801 gtcgctcgcc gcgctgtgcg agcagatgac cgaggaggcg caccggaaca tgctggccac
     1861 caaggacatg atcgccacca agggtcgcgc ctactttctc ggcgagcgtg ccctcggcca
     1921 tatcgacccc ggctgcaaga cctgcgaggt ggcgatcgcc gccgtgtgca acaccgtgtt
     1981 ggcgaaggag cccgcatgaa gaagttcatc aacgacatcg acagcgtgat gcgcgagtcg
     2041 cttggtggcc tattggccgc gcatccggat ctgctgcgcg ttgagttcga gccgaccttc
     2101 gtcgcgcgag ccaccaaggc cctgaacaag gtggcgctga tctcgggagg cggctcgggc
     2161 cacgagccgt tgcatgccgg cttcgtcggt ggcggcatgc tcgatgccgc ctgcccggga
     2221 caggtgttta cgtcgccgac ccccgaccag atgctcgcgg ccgcgcagag cgtgcatgcc
     2281 gatcgcggcg tactcttcat cgtgaagaac tacgccggcg actcgatgaa tttccagatg
     2341 gcagccgaga tgctggaaat cgagaacgcc accgtgctgg tcaacgacga cgtggcggtg
     2401 gagaactcca cgcacaccgc cggccgacgc ggcgtggcgg gcaccgtgat ggtggagaag
     2461 atggtcgggg cggctgccga agccggcgcg tcgttgcaag aatgcaaggc aatgggcgac
     2521 cgcgtcaacg cggccaccgc gtcaatgggc gtcgcgttca ccagctgcac ggtgccggcc
     2581 gccggccgcg cgaccttcga gatcggcgac gacgaaatgg aggtgggcgt gggcatccac
     2641 ggcgagcccg gccgccgacg cgagaagctc aagcccgcgg cggcgatcgt cgaggaactg
     2701 ctgagctcga tcctggcgga cctcaagccg cagcgtgggc gcgaggcatt gctgcacgtc
     2761 aacggcttcg gcggtacgcc gcagatggag ctgtacctgc tgtaccactg cgctgccgag
     2821 cagctgcgca agctggggct gcagccggtg cgctcgctgg tgggcaacta caccacgtcg
     2881 ctcgagatgg ccggtgcgtc gctcacggtg acgctgctgg acgacgagat gaagtcgctg
     2941 tgggatgcac cggttcatac gccggccctt cgctggtaga gccgtgaagc ggcgcgccgt
     3001 actctgctgg gccggcagcg cggtgctgcc gccgctggcg tggcccttga acgctgccgc
     3061 catcgcgacg gagcgctcac cgatccgcct gggtcagtcg gcgccgatca ccggaccgtt
     3121 ggcgcgcgtg ggaacggcct tccgggatac agcccgcgca gtgttcgccg aggccacggc
     3181 ggccggtggt gtcgcgggcc gcgccatcga gctcgtcacg ctggacgatg aagaccggcc
     3241 cgagcgcacg gccgtcaacg taaagctgct cgcgtccgag tacaacgtgg tggggctttt
     3301 tggcttcgtc ggcgcagggg cgcatcgagc cggcgcgcgc ggcgcggcgg acgaaggcct
     3361 gccctacatc gccgcggtca gtggctcgca ggagctgcgc tcgggcgctc tgccatggat
     3421 cttcaatctg cgcgccgggc atctggacga gatcgagtac atcgtgcgcc acacacagca
     3481 ggtgggcatc acgcgcgtgt cgctggtctc ggaatacaac tcgcagggct gggagctgcg
     3541 cgacgcgctg atcacctcct tcgaggcacg cggcagccag gtcgcctcga tcagcagcat
     3601 cgaccatgaa ggcagtcgct acagcctgcc cggcgcggtg tcgtcggtat tggccggcgc
     3661 gccgcaggcc atcgtgttgg gtgcggacta tgtggccagc gcgcgctttg tgacggccgt
     3721 gcgcgaggcc ggcttcactg gactcttcta caccttgtcg acggtcggcg gcgaagccat
     3781 gacggagcag ctcggcccgc gggctgtggg tctgtcggtg acgcaggtcg tgcccttccc
     3841 gtggacggcc tcgctgccgg tgggccgcag cttccagagc ttttgcacgc gccacaagct
     3901 cgagccttcg ttcgccagca tggaagcgtg gcttgccgcc aacctgctgg tcgacgcgct
     3961 gcgccgagcg cgcgacgcga cgcctgccgg gctcgtcgat gcgttgcagg cgctgcctcc
     4021 gcgcgacttc ggcggctacg tcgcggcgtt ctactcgagc gccagatcgc gccgcacgcc
     4081 gccgcaggtg gatctcacgg tgttctcgcg cgcgggcaag ttcatcaagt gatcgcaatg
     4141 aagctcgtca tcggcaactg gaagatgaat ggatcgctcg ccgcgctgcg cgagttcgct
     4201 caggcggctc agggctcgtg gccggcatgc gaggcggcgc tgtgcgtgcc gttcccgttg
     4261 cttgcggcgg ctcagtcggc gctgcaggcc acgccgctgc gctggggcgc gcaggactgt
     4321 gcagcgcacg gcgagggcgc ctacacaggt gacgtgtcgg ccggcatggt cgccggcttc
     4381 ggcacgcgct acgtgatcgt cggtcattcg gagcgccgca tccgtcatgg cgagaccgac
     4441 gccgaggtcg ccgagaaggc gcggtgcgtg attgcggctg gcatgacgcc catcttgtgc
     4501 gtcggcgaaa ccgaggcgga gcgtgacggc aaccagacgc cggcggtgct gcgccgccag
     4561 ctgttgcagg cgctgagcgt gctggggcgc gacttcgtgc gcgcgatcgt cgcctatgag
     4621 ccggtgtggg cgatcggtcg cggcgacgcc gctacgccgg gcctgatcgc agacacgcac
     4681 ggactcatcg agcagacgct gatgatccat gcgggactat ccgcgcaggc ggtgcgcctg
     4741 ttgtacggcg gcagcgtcaa cgcacgcaac gcgtccgcga tcctgtcgac cccgctcgtg
     4801 gcgggcgccc tggtcggcgg ggcgtcgctg cggcctgcgg agttcgtcgc catctgccgt
     4861 gccgacgagg ctcgggcatg aggtgaggat gcagctgaag gcgatcgtcg ggcctacgta
     4921 gttgctggcc gcggccattg gcggctcgat caaggtcgac agcgtcgcag acttggcgat
     4981 tgccatcgct gcctcgctgc ctcgcggccg gcggctactg ccgctgaagg gcgaaggccc
     5041 tggccgcaca gttgcgcgca cgtagcggaa cctccagtcg ctgtttgtcg cggcgcatgg
     5101 ccgctaatgg gtgcccatgc gctcgcctcg gaatcaccct gcctgccacc ggcttgtgat
     5161 tgatggtttt tactgatcga tttatttact gattgatata tagttgtatg gaacaagcaa
     5221 attgcagggt aggtcttcag gcggtctgag ggtccgcgca ggaggcgagc tatgaacgca
     5281 ctgcacatgc atctgaggct ctacggcgtg ctcgacgttg cgcgccgaca cccgaagctc
     5341 tttctgcgcc tcgtggcgat agggcacgcg tgaggcttcc acccgcgagc cggagcgaca
     5401 cccagcaggg gcagcgcctg ccgcgggcgt ggcaggcaag gagcatggcg atgtgtattc
     5461 gggttgccca agacgatgag ctgcagtcgc gcactcgcgc gcgctgcgat gatcctgcgg
     5521 cggctccggg gcgcgaggcg ttgagcccgc tgctggaggt ggccgtcggc gtggccgatg
     5581 cgaaaggcgc tgccctgttc ctggcggctg atgagcagcc gattctcgtc ggtacgatcc
     5641 cgctgccggg ctggcccggc atccggcgga tgagcgagtg cgtcggcgca gcgcagggcg
     5701 gtaccggccc cgatgtcgag ctagaggact gcggtgggct gatcagagtc accctgcgcg
     5761 aaacgccgca ggcaccggcg cagcggctgg tggtctgggg tgttccgccg cttgccggca
     5821 cgcagcgcgc aaagctgctc ggcgccgcgc gcgcattgct cgcctatctg cgcaccgagc
     5881 gccaggtgcg cgctgccgcc ctgaacgcga ggcgcgtgca ggacgtggcc tatgccagcg
     5941 gcgaatggct gtgggagacg gatgctgagc accgctatac ctgggtgatg cgcgatccat
     6001 cccgcggtcc ggatggggcc agcgagccgc gggtgggtag cgagatcgcg caggcgtacg
     6061 tcgtcgattg tttcggcatg cccgaggtgc ctgagacgac cttgcgcgag gtgctggctg
     6121 agtgtcgccc catcgtgcgc ctggtggtgg gcgaatccga cggtacgcgc atccgctttg
     6181 tgtcgcgcag cgccgtgccg atcgtcggta cggacggtgg ctttctcggc tatcgaggat
     6241 ccgcgcgcga tgtgacagac agcgtgcagg ccaaggccga gctctggatg cgcgacgagc
     6301 agctgcgtcg ctcggcgcag gcgttgcccg gggccgtctt gcaagtcggc tgcggcagct
     6361 ggctgtcggc ccgcatcgag gatcacagcg ggcgcttggc ggagctactc ggcctgccgg
     6421 gcgcgctgcg cagctacccg ctgcgcggcg cgttgcgcaa gttgcccgca gccgagcgag
     6481 cgcgcctgca cgaggtggcg cgctcaggcg tcccgacgct gttcctgcgg ttgcgcacgc
     6541 tgtgcatgcc gcccgctgcc agctggatcg aattgcatgc cactgccaat cgcaaggcgg
     6601 gcggcgccac ctcctggagc gtcttcatct ccgatgccac gcaacgcgtg cgcgacgagc
     6661 aggcgctgcg ggcacacgag cagcgttggg gtctggctgc aggcgcagcg gggctcggct
     6721 tgatcgagat cgacctggta gccggctgcg cgacattcga cgagcgaagt gtggccctta
     6781 ttgggacgtc caactgcgca gccggtcgcc agacgcagct gcacgacctg ttggagaggc
     6841 tggatgcaga agggcgcgag cgcctggtcg aacgtttggc tgacccgcta gccgacgcgt
     6901 ctgcgctgtc gctggaactg gatggcggcc ggcatctaaa catgctcgta tgcccgccgg
     6961 aaccgtcgaa ccctccaggc ggccgcctga cagtgatctg ccgcgacacc acgagcgagc
     7021 accaaatcgg gcggctgagg ctggcgaagg aagccgccga ggcggcgagc cgcgcgaagt
     7081 cgatgctggt gtccaaggtt ggccacgaac tgcgcacgcc gctgaacgcg atcgttggct
     7141 tgtcacagct gatccgcctg aagcgcgacg ggctgcagga gacagcctcg gtggacgggt
     7201 ggatagagca gattgccagt acgggtcggc acatggcggg tgtgatcgaa accttgatgc
     7261 agctcgggca gtccggcgcc ggtcgactgc agatcgctga acagagcttc gacgtgcgcg
     7321 agccgatgga agaagcgcgc agaatcctcg agcaggaggc agcgcagcgc ggggtggcca
     7381 tcgaggccga tgccgcgttg ccggtgaagg tgctcgcgga ccgccgggcg gtgcggcagg
     7441 tatttctgaa cctgctgagc aacgccatca aatacaaccg cgagggcggc ggcgtgaggg
     7501 tggtggtgca ggatggcacg cgcgttcgca tcacggtgca tgacacgggg ccggggctca
     7561 gcgccgagca gcgcttacgg ctgttccagc ccttcgaacg cctgggggcg gaaagcagcg
     7621 gcgtggaagg gtacggcctg gggctgctgg tgtgcaagga gctgctggcg gcgatggatg
     7681 gcacgatcga cgccgagagc gtgcccggtg ccggttgcgc attcatcgtc acgctgcctg
     7741 gtgtcgccga atgcgccgcg agcccgacca aggcggaatc cgacgtcgga tgaacccgac
     7801 atgtcgcgta tcgggccccg gcctccgatc cgatgaccgg aagctgcact ggccaattgg
     7861 tgccgttccg cgtgatccaa ggcggctaca tggtcgcaac ctgagagcgg ctatcgcttt
     7921 aacgaaagct ggagcctggc gctgaacgtc gacaacctgt tcgaccgccg ctactaccgg
     7981 accatcggct acacgggcgg cgacaactgg tacggcgcgc cgtgcaactg gatgctcacc
     8041 ctgcggggcg agctctgatg gcgcctagac ctgatcgagc gccattgtgt tggcgctccc
     8101 gcaagcatca cgccccgtcg ggcgagcggc gtgttgggct ggtgccccaa gctgcagctg
     8161 acggccgaga ttgagccaca gcaaagggcc gacgcgggac ggttctggcc tggcaggctc
     8221 aggtcagcca ggctgttgcg cccacccagg gtgcgggcag gtccagcatc gcgagccggc
     8281 agcgtagcag gcgtgctgcc atcgcttcac tccattcatc gagcggctcg ccaagacact
     8341 tcagtgcggc ctcgtggcga caccaagccg cggcgaaggc ctgttcacgt ttgggaacag
     8401 ccactgccgc cagggtctgc gcgacttcgg ggccgaggta ttggcttgcg aggttttgca
     8461 tctccggcaa cgggatcggc agcatcagat ccacgcccac ttcaccctgg ggccataagg
     8521 ccagcagcga gcagccctcg gcatggctga tcgagatctc gatcggcgct tcagggtgat
     8581 cgaggcgtat cggctggccc ggcgtgcccg acagcgccac cgcctccgct gcaatgccga
     8641 agtgggcacc gagtaagcca cgcagggccg tgcgcacctg ctccctggct cgttcccgcc
     8701 ttgtccccct cggcagcttc agggccagca cggtcggctg cgtggcgctc agtcggggcc
     8761 aggcgcctgc tgcatcgggc agcgcatgca ctgccagctt catcatcgct actacgctgc
     8821 cggagccgcc gggcagccgc gccaggacga gccggccggc aactgctcgc ccttcatcgc
     8881 cagcgtcagc ggtccgagcc atgcatcgtc gccgaccgag gcgccgtaga gcacggtgct
     8941 gcgcgcgccc atcgtgacgc gcgcgccgat atcgaccacg tcgatcttca tcacgcggtc
     9001 ctcgaacaga tgcgtctgcg ggcagctcag cgcattcagc tcgctgtagt cgccgacgcg
     9061 cacgcagtcg aattcggtga tgtcggtggt gtcgaggtag acgccgcggc cgatgcgcgt
     9121 gcccagcagc cgcagggccc agggcagcca gggcgtgccg cgcaggtagc gcacgaagtt
     9181 gggcacggcg atgccttcgt agagattggt caccgcctcg gagaaccaga cgaagcgcgt
     9241 ccacatcggc acagcatgcc ggctgtagcg gccgatcagc agccacttga gcaaggccac
     9301 gaaggccagg ctgcccaggc cgtagagcag gccggccagc gtgaggccga tcacgacctg
     9361 cccccagtct tcttcgccgg ctgcgggcat caggtcgagc acgatggtgt agccggtcgc
     9421 gatcacgatg gcgtgcggcg cgacgatgcg cagcgcctcc accaggccgc ggccaagacg
     9481 gcgcagcggc gaggggcgga aggtcagcgc atcgggatag cccgccacct gctcgcgcgc
     9541 cggcaggttg atgggcggcg agcccagcca ggtgtcgcct tcgcgcatct gcgcgttctc
     9601 gggcacgcgg gtgtgcacgc cgatcagcag gttctcgggc agcacgctgc cgtcgggcac
     9661 ataggcgccg ttgccgacga agctgcggcg cgacaccacg gtgggctgca tcgtcatcca
     9721 gccgccgtcg atctcttcgt cgcccagcag cacgttgtcg gcgatgaagg tctcgtcgcc
     9781 cagcgtgagc atgtccggca ccacgccgag cgcggtggag atctcggcgt ggcgcccgac
     9841 cttggcgccc agcagccggt accagaaggg cgcatacacc gtggcataga cgccgtgcag
     9901 cacctgcagg ctggtctcct ggatctggtt gaccagccat ttctcgtaat aggtgcggct
     9961 gcgcaccggc cagctgcctt cgcccaggcg cggcaggaag gtccagcgca gcgccgccga
    10021 gatcagggca gtcagcagga tcagcaccgc gctcgcgggc agcgagagca cgaagtactt
    10081 gagcagctgt atccacacca cattgccctg cagccagggg aagcggtccg cgtcgtccag
    10141 ccagtcgatc agcatgaagg ccgggaacac cggcatgaag aacagggccg cgatcagcag
    10201 gctgccggcc gagaaataga accattcgag cgccgggcgc caggcttcga ctgcggggcg
    10261 cggtggacgc gacgcgggat cgaagacgcc ggtctcgcgc gccggcgagc cgctccagat
    10321 gcgccgcgcc gggatctgct gcccgtcggc cagcgccgag atgccgtcga ggtgcgcgta
    10381 ctcgcccagg gtggtattgc cttccagcac cgagaacgag cccacataag cgttggcagc
    10441 gatctcgatg cggccgagga tgagctcgcc gccctcgacg cgcgcgttct ccagattgac
    10501 ggccgtgccc aggctggctc cgtcgccgat gctgagcagg tcgggcgcgc gcagggtcag
    10561 cgagccgatc tgcgcatcgc gcccgaccct ggcgcccaag gcgcgcagat acatcgggaa
    10621 cagggagctg cccggcagca tgtgcgtggg cgcggcctcg atcagccggt ccgacagcca
    10681 ccagcggaaa taggtcagcc cccacagcgg atagcggccg ggccgggcgc ggcccagcac
    10741 cagccatttg ccggcgatgg tcacgacgaa ggccagcatc gtcgccagca ggaaggccgc
    10801 cagcgatatc gcgacagcga aggcgatcga gtcgttgggg ccgccggtga agaagtgata
    10861 ggtgaagaag ggcgccagcc attgcgccat gcgcagcgcc accagtggcg gtaccgccag
    10921 tgcctgcgcg gcgccgcaca gccaacgccg caatgcgggt gtggtcgtga aggctcttgg
    10981 cttcgcggct gcctgccccc gcctgagctc ggcctcgccc atggcctgcg cgatcgcgcc
    11041 caggcggcgc tgctggtaga tatcgcgcac cgagaactgg gcatagtgtg cgtcggcccg
    11101 gattgccgac gcgaggcggg ccgcgaacag cgaatggccg ccgagatcgg agaagaagtc
    11161 cgcgtcgcgg cgtagcggca aacccggaaa gagctgctgc aaggcagcga acagcaactc
    11221 ctcggccgcc ccctcggggc gatcggattc gccggccgcg ggcgcgatgc tgagccggcg
    11281 cgccttcaag tccttgcggt cgatcttgcc ggagggcagg cgcggcatct cgctcaagcc
    11341 ctcgtagcgg ctgggcacca tatagggcgg cagcacggct gccagcgccg tgcgcagcgc
    11401 tgcggcatcg atcgccgccc cgtctcgagg taccaagaaa gcgaccagct gctccagccc
    11461 atcgctgttg tgcagcacga ccgccaccgt gccgacgccg ggctggcgcg cgagctgggc
    11521 ctcgatctcg cccagctcga cgcgaaagcc gcgcagcttg acctggtcgt cggtgcggcc
    11581 caggcattcg acgcggccgt gcgcgtcgat gcgcgccagg tcaccggtgc ggtacagccg
    11641 cgcatcgtgg tcgccgttgg cccagggatt ggcgaggaat ttctcggccg tgagctcggg
    11701 ccgccccaga tagccggccg ccacgcccgg accgctgatg cacagctctc ccacttcgcc
    11761 acgcggcagc agcttgaggc cctgtccatc gacgacctcg ggcgcgacga ccagcagcac
    11821 gtagttgggc agcggctcgc caatcgttac cggctcgccg gcatgcagct cggccatgct
    11881 ggccgacacg gtggcctcgg tcggcccata gctgttgaag acctggcgcc cgggcctggc
    11941 ccagcgtgcc accagcgcct gcgggcacat ctcgccgccg aggttgatca gccgcaggct
    12001 cggcacgtcc tgcgcgaaga gcgcgagcag cgtgggcacc gcgtgcagca ccgtgatctg
    12061 ctgctcgatc aaggccgccg gcaaggcctc gggatcgctc gcaatctcgc gcggcgcgat
    12121 ccaaaggctc gcgccgacga gataggagat ccagatctcc tcgaaggaca tgtcgaaggc
    12181 gaccgagaag ccctggtaga cccggtcgga ctggcgcacg cccagccgct cgttctcgct
    12241 gcgcaggaag tggcagatgc tgccctggct gatcacgatg cccttgggtc tgccggtcga
    12301 gcccgaggtg tagatcacat aggccgggtc gttggcctgg accgcggcgc gccgctgcaa
    12361 tgcgcctgat ggcctgaggc tcagcgcctc ggccgtcaat acgggttggg gcagtgcatc
    12421 cagccgcggc agccaagcct cgcagctgag caggcaggct gatttcgcgt cgtcgaggca
    12481 gaccgcgatg cgctccaccg gcgtgtccgc atcgaagggc agccaggccg caccggcttt
    12541 cgcaatgccg gcctgcgcga ccagcaggtc caagcacctg ggcagccaca ggccgacgat
    12601 ggagcccggc ccgacgccgg cggcgatcag gttggacgcg acgacgctgg ccagctcgtc
    12661 gagctcggca tagctgagac tgcgctgct
//
LOCUS       DASUCY010000325         3912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1436659, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000325 DASUCY010000000
VERSION     DASUCY010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1436659"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VFV25_06365"
     CDS             <1..239
                     /locus_tag="VFV25_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392979.1"
                     /translation="KILVGNRANPSDFNVMILTEGPNWASLDGLDAKFDAIAAKFAGS
                     ADKATEMDKKTMTDRAKIRTIFGSKMMQQVEFKK"
     gene            complement(306..725)
                     /locus_tag="VFV25_06370"
     CDS             complement(306..725)
                     /locus_tag="VFV25_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEY1392980.1"
                     /translation="MKILIAADGSKYTKRMLAYLAARDEWREGHDYTVLYCVAAVPHR
                     AAAFAGADRVRDFYNDDAATVFRPIRSFFEKQGIPARFVHKIGPAAEHIAKLAERGKF
                     DLLVMGTHGHGALGNLVLGSVTTKVLALSSTPVLLVR"
     gene            complement(927..1520)
                     /locus_tag="VFV25_06375"
     CDS             complement(927..1520)
                     /locus_tag="VFV25_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010393679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HEY1392981.1"
                     /translation="MDIELPSAASEVLDFWFGTPGSPDYLQPRKAWFSKNLDFDAEIR
                     ERFGSRIEAAIAGALDAWTATPPGALARILLLDQFTRNSWRDTPRTFAGDAQALAAAR
                     AMVATGQDLQLPPVQRGFAYLPYEHAEDLAAQDESVRLFTALAQQAPDQAEDLDYAHR
                     HREIVRRFGRFPHRNAILGRTSTPEELAFLKQPGSSF"
     gene            complement(1524..1811)
                     /locus_tag="VFV25_06380"
     CDS             complement(1524..1811)
                     /locus_tag="VFV25_06380"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY1392982.1"
                     /translation="MSAAEKIQVVHNSAASRFEAKVQGELCVADYRLHEGVVTFTHTE
                     VPPTLRGHGVAAALVREALAWCAAQGHRVIPACSYVRAYIQRHAELQPLLA"
     gene            1932..2486
                     /locus_tag="VFV25_06385"
     CDS             1932..2486
                     /locus_tag="VFV25_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014752619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEY1392983.1"
                     /translation="MSYCEIAPGHPEHGPYHDREYGFPLRDDKQLFERLVLEINQAGL
                     SWLTILKKREHFRRAYAGFDIDAVAAFGEHDRERLLNDPGIIRNRLKVDAAIENALRL
                     QAIRAEQGSFAAWLDLHHPMDKAGWVKLFKQTFRFTGGEIVGEFLMSCGYLPGAHDES
                     CPVHARVLAARPPWLLANKKKQKR"
     gene            complement(2483..3298)
                     /locus_tag="VFV25_06390"
     CDS             complement(2483..3298)
                     /locus_tag="VFV25_06390"
                     /inference="COORDINATES: protein motif:HMM:NF012995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HEY1392984.1"
                     /translation="MTSPAVDLDAYLDRVQWGGALRPSYDTLAGLLRAHMSRIPFENL
                     DVLLGRPIRLDLEGLQKKLVQAHRGGYCFEHATLFAAVLEQLGFRLVRHIARVVLITP
                     RHASPRTHMFLTVPLAEGRFVVDAGFGALAPTFPVPLIAKPADPPEPTTHWMARDGKL
                     WVLRARMGGKPPVDAWVSTLEQEPAVDFEMGNHYTSTHPDSPFVNRILMRALTPDGRV
                     SVMNRDLTVWRDGQPEALQIADRAALRALLNEHFGFDLPEVERLRVPAIPEWR"
     gene            3614..>3912
                     /locus_tag="VFV25_06395"
     CDS             3614..>3912
                     /locus_tag="VFV25_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HEY1392985.1"
                     /translation="MRLKMLSLLVLMSCASIATQAGVTDQTIENGAKTGSGVLSWGLS
                     TNGERYSPLKQVNAGNASRLSPVWAFSFGGEKQRGQESQPLVYNGKMFVTASYSR"
BASE COUNT          675 a         1256 c         1336 g          645 t
ORIGIN      
        1 acaagatcct ggtcggcaac cgtgccaatc cttcggactt caacgtgatg atcctcaccg
       61 aaggccccaa ctgggcctcg ctggacggcc tcgatgccaa gttcgatgcc atcgcggcca
      121 agttcgcggg ctcggccgac aaggccaccg agatggacaa gaagaccatg accgacaggg
      181 ccaagatccg caccatcttc ggcagcaaga tgatgcagca ggtcgagttc aagaaatagc
      241 ctcgcgggcc cccggcggct ccgggggcat cgcccctcgt gcaggtgtgc tgcgggcaag
      301 ggccttcagc gcaccagcag caccggcgtc gaggacaggg ccaggacctt tgtcgtcacc
      361 gagcccagca cgagattgcc cagggcgcca tgcccgtgcg tgcccatcac cagcagatcg
      421 aatttgccgc gctcggcgag cttggcgatg tgctccgcgg cagggccgat cttgtggacg
      481 aagcgggccg ggatgccttg cttctcgaag aagctgcgga tcggcctgaa gacagtggcc
      541 gcatcgtcgt tgtaaaagtc gcggacccgg tccgcaccgg caaaggccgc ggcccgatgc
      601 ggcacggcgg ccacgcaata gagcaccgtg tagtcgtgcc cctcgcgcca ttcgtcgcgc
      661 gccgcgagat aggccagcat gcgcttggtg tacttgctgc cgtcggcggc gatcaggatc
      721 ttcatggtgt gctcccttta tgcgccccca gacctgccgc tgcgcgtcag gcccccccga
      781 gggggtcaag aaacctcggg agcggccctt cggcgtcttg agagatatcg agcacaccat
      841 tgtgaggacc gcgacgccgc cggacccgag ctggatcaag cttccattgc gatgcggcgc
      901 tgcgcgacgg cagcgctttc cggtcgtcag aagctggagc ccggctgctt caggaaggcc
      961 agctcttcag gcgtcgaggt ccggccgagg atggcgttgc gatgcgggaa gcggccgaag
     1021 cggcggacga tctcgcggtg gcggtgggcg tagtccaggt cttccgcctg gtctggggct
     1081 tgctgggcta aggcagtgaa gagccgcacc gactcgtcct gggcggcgag gtcttcggcg
     1141 tgctcgtacg gcaagtaggc gaagccgcgc tgcacgggag gtagctgcaa gtcctgcccc
     1201 gtagcgacca tggcccgtgc ggcggccagg gcttgtgcat cgcctgcgaa ggtgcgcggc
     1261 gtgtcgcgcc agctgttgcg tgtgaactgg tccagcagca ggatgcgagc cagcgcgccg
     1321 ggtggcgtcg cagtccatgc atcgagggcg ccggcgatcg ccgcttcgat gcggctgccg
     1381 aagcgttcgc ggatctcggc gtcgaagtct aggttcttgc tgaaccaggc cttgcgcggc
     1441 tgcaggtagt ccggcgagcc gggcgtgccg aaccagaagt ccaggacttc gctcgcggcc
     1501 gaaggtagct cgatatccat gagctaggcc agcagaggct gcaattcggc gtggcgctgt
     1561 atataggcgc gcacatagct gcaggcgggg atcactctgt ggccctgcgc ggcacaccag
     1621 gccagcgcct cgcgcaccag ggccgccgcg acgccgtggc cgcgcagcgt gggcggcact
     1681 tcggtgtggg tgaaggtgac gacgccttcg tgcaagcgat agtcggccac gcagagctcg
     1741 ccctgcacct tggcctcgaa gcgcgaggcc gcgctgttgt ggacgacttg gattttttcg
     1801 gcagcactca tgtcgttgcg atcgagagga aacggcgtta gtgtcgcaaa gcggtcagca
     1861 ccttgctcgg gcaaggccga tctctggcag gatgccatgc ttcaaatatc gagaagcatc
     1921 gagaaccgag catgagctat tgcgagatcg cccccggcca tcccgagcac ggtccctacc
     1981 acgaccggga gtatggcttt ccgctgcgcg acgataagca gctgttcgag cgtctggtgc
     2041 tggagatcaa ccaggccggg ctgtcctggc tcacgatcct gaagaagcgc gagcacttcc
     2101 gccgcgccta tgcgggcttc gacatcgacg ctgtggcagc cttcggcgag cacgatcgcg
     2161 aacggctgct caacgatccc ggcatcatcc gcaatcgcct gaaggtggac gcggccatcg
     2221 agaacgcgct gcggttgcag gccatccgcg cggagcaagg gtccttcgcg gcctggctgg
     2281 atcttcacca cccgatggac aaggccggct gggtcaagct cttcaagcag accttccgtt
     2341 tcaccggcgg cgagatcgtc ggcgagttcc tgatgagctg cggctacctg cccggcgccc
     2401 atgacgagag ctgcccggtg catgcgcgcg tactggcggc ccggccgccg tggctgctgg
     2461 cgaacaagaa gaagcagaag cgctagcgcc actcggggat agcaggcacg cgcagccgct
     2521 cgacctccgg cagatcgaag ccgaagtgct cgttgagcag ggcccgcagt gccgcccgat
     2581 cggcgatctg cagggcttcg ggttggccat cgcgccagac cgtgaggtcg cggttcatca
     2641 ccgaaacccg gccgtcgggc gtcagggcgc gcatcaggat gcggttgacg aagggcgagt
     2701 ctggatgcgt ggaggtgtag tggttgccca tctcgaaatc caccgcaggc tcctgctcga
     2761 gcgtggacac ccaggcatcg accggcggct tgccgcccat gcgggcgcgc agcacccata
     2821 gcttgccgtc gcgcgccatc caatgcgtcg taggctcagg cggatccgct ggcttggcga
     2881 tcagggggac ggggaaggtc ggcgcaagcg cgccgaagcc cgcgtcgacc acgaagcggc
     2941 cttccgccag cggcaccgtg aggaacatgt gtgtgcgcgg cgaggcatgg cgcggggtga
     3001 tcaggaccac gcgcgcgatg tgacgcacga gccggaagcc gagctgctcc agcacggccg
     3061 cgaacagcgt cgcatgctcg aagcaatagc cgccgcggtg cgcctgtacg agctttttct
     3121 gcaagccttc caggtccaac cggatcggcc ggcccagcag cacgtccagg ttctcgaaag
     3181 ggatgcgcga catgtgcgct cgcagcaatc cggccagagt gtcgtagctc gggcgcagcg
     3241 cgccgcccca ttgcacgcga tcgaggtagg cgtcgaggtc gacggcaggg ctggtcatgt
     3301 tcgttctttt ccttcggtga tgccgggcca catggtgccg gcacgcaggc cgttcttcgc
     3361 ccgcatcgcc gtgatgccat ggccgcggcg tgggaatgct actgctcaag cctgtagcac
     3421 tgcgatcttg cgaattggcc gttgctacgg cctgcaacag tcccgatccc tcgcggcacg
     3481 gcgatcggta ggaaaacccg cccctgccgc ctgccacgcg ggcttgatcg agtggcacag
     3541 agcttgcgct tggctagctg cgcgccgctc gcgggctggg cagcgcacgg accacctaca
     3601 ggagacaaac accatgcgct tgaagatgct ttccctcttg gttctgatga gttgcgcctc
     3661 gattgcgacg caggccggcg tgacggacca gacgatagaa aacggcgcca agaccggcag
     3721 cggcgtgttg agctggggcc tgagcaccaa cggcgaacgc tattcgccgc tcaagcaggt
     3781 caacgccggc aacgcctcgc gcctgtcgcc ggtgtgggcc ttctccttcg gcggcgaaaa
     3841 gcagcgcggc caggaatccc agcccctggt ctacaacggc aagatgttcg tcacggcctc
     3901 gtattcgcgc at
//
LOCUS       DASUCY010000326         2361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_360506, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000326 DASUCY010000000
VERSION     DASUCY010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360506"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..426
                     /locus_tag="VFV25_06400"
     CDS             94..426
                     /locus_tag="VFV25_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1392986.1"
                     /translation="MRIFRPNRVALGAIAALSLTLAGCYVVPADPYYQGAEVVTVAPP
                     PAQVEVVGVAPYPGYIWIGGYWGWSGYSHVWYPGYWSAPRPGYRWYPHSWDRSGNGWR
                     ESRGHWGR"
     gene            complement(472..1194)
                     /locus_tag="VFV25_06405"
     CDS             complement(472..1194)
                     /locus_tag="VFV25_06405"
                     /inference="COORDINATES: protein motif:HMM:NF025433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LssY C-terminal domain-containing protein"
                     /protein_id="HEY1392987.1"
                     /translation="MSRPALRIAALLAALALGAALIYGLTAYEFVPSVWRFVERRHPA
                     LDRMGTCAFTKVGIPGDPLNLAFIGSEEALQRAMLRAGWQPADPITLKSSLRIAVDSV
                     VHRPYLDAPVSDLYINGKKQDLAFEQAAAGDPSKRHHVRFWIAAERDALDRPLWMGAA
                     TFDSGIGLSHETGQITHHIAPEVDAERDKLLKDLQALGSLSFRWIDEFQPQRSGKNGG
                     GDRYVTDGRLAVIVAGEAASHP"
     gene            complement(1229..1963)
                     /locus_tag="VFV25_06410"
     CDS             complement(1229..1963)
                     /locus_tag="VFV25_06410"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HEY1392988.1"
                     /translation="MPLSFPAARSASKTKLITRCAAAALLAGASTFACAQAAGSNVIT
                     SGWLHFEPRTSSDPFVMTSPVNQVLPGTGAKVHNTNTVSLAYERYVTDNWGIKFAGGW
                     PLANKIEGTDTLAGFGELGQADQYSPTLLAVYHFGEAKSTLRPYFGLGFNRTWFRNAK
                     ITNAAFSAAVFGPGASTNVSIEPTWNPVANAGVNYRFNDTWSLGFSLTYLPLKAKATV
                     SSSTVTVQDNLKSQGIASDLFVGYSF"
BASE COUNT          364 a          732 c          845 g          420 t
ORIGIN      
        1 gcccgcccgg ggcggggttg tggctgcgtc gcgtcatcgg tttgagcccc gccagcgtta
       61 agggggtact cttggcatcg agtcgaggac tacatgcgca tctttcgccc caaccgcgtc
      121 gctctaggcg ccatcgcagc cctgtcgctc acgcttgcag gctgctacgt ggtgcctgca
      181 gacccctact accagggcgc cgaggtggtg acggttgcgc cgccgccggc ccaggtcgag
      241 gtggtgggcg tcgcgcctta cccgggctac atctggatag gcggctattg gggctggtcg
      301 ggctatagcc acgtctggta cccgggctat tggtcggcgc cgcgtccagg ctaccgctgg
      361 tatccgcata gctgggatcg ctccggcaac ggctggcgcg aatcgcgcgg ccactggggc
      421 cgctgaccag cctcatcggc gccggctcgc cgatgagctg tccgcctgct gtcaggggtg
      481 gctcgccgct tcgccggcga cgatcacggc cagccgcccg tcggtgacat agcggtcgcc
      541 gccgccgttc ttgccgctgc gctgcggctg gaactcgtcg atccagcgga agctcaggct
      601 gcccagggct tgcaagtcct tgagcagctt gtcgcgctcg gcatcgactt cgggcgcgat
      661 atggtgtgtg atctggccgg tctcgtggct caggccgatg cctgaatcga aggtcgcggc
      721 acccatccac aaggggcggt ccagcgcgtc gcgttccgct gcgatccaga acctgacgtg
      781 gtgccgcttg gacggatcgc ccgccgcggc ctgctcgaag gcgaggtcct gcttcttgcc
      841 gttgatgtac aagtcgctca ccggtgcgtc gagataaggc ctgtgcacga cgctgtcgac
      901 cgcgatgcgc agcgaactct tcagcgtgat cggatcggcc ggctgccagc cggcacgcag
      961 catcgccctc tgcagggcct cctcgctgcc gatgaaggcc aggttcagcg gatcgcccgg
     1021 tatgccgacc ttggtgaagg cacaggtgcc catgcggtcc agggccggat gccggcgctc
     1081 cacgaagcgc cagaccgagg gcacgaactc gtaggcagtc agcccgtaga tgagcgccgc
     1141 gcccagagcg agcgcggcga gcagggcggc gatgcgcagg gcgggtcggc tcatgccaag
     1201 cccccgcaga tagtcacccg ctagagaatc agaagctgta gccgacgaag agatcgctgg
     1261 cgatgccctg gctcttcagg ttgtcttgca ccgtgaccgt gctcgaggac acggtggcct
     1321 tggccttcag cggcagatag gtcagcgaga agcccagcga ccaggtgtcg ttgaagcggt
     1381 agttgacacc ggcattggcg accgggttcc aggtcggctc gatcgacaca ttggtgctcg
     1441 cgcccgggcc gaacacggcg gcgctgaatg cggcgttcgt gatcttggcg ttgcggaacc
     1501 aggtgcggtt gaagcccagg ccaaagtagg ggcgcagcgt gctcttggcc tcaccgaagt
     1561 gatagaccgc cagcagcgtg gggctgtact ggtcggcctg gcccagctcg ccgaagccgg
     1621 ccagcgtgtc ggtgccttcg atcttgttgg ccagcggcca gccgcccgcg aacttgatgc
     1681 cccagttgtc ggtgacgtag cgctcgtagg cgagcgacac cgtgttggtg ttgtgcactt
     1741 tggcgccggt gccgggcagc acctggttga ccggcgaggt catgacgaag gggtcgctgc
     1801 tggtgcgagg ctcgaaatgc agccagccgg aagtgatgac attgctgccg gccgcctggg
     1861 cgcatgcgaa ggtagaagcg ccggccagca aggcggctgc ggcgcagcgg gtgatcagct
     1921 tggtcttgga ggcagagcgg gcggcgggga aagaaagagg catcaagggt ctccgtgtgt
     1981 gcaagatttg ttggttgtga ggccacgagg ctcaactggc gattgtcgcc gcggccaaca
     2041 gcggaggccg tcatttcgtt gggcgggcgc gacgcttgcc acgtttgggc gctggtgagg
     2101 cgggtcggga taccggctcg ttggccctgt aagttgccgg cttgtcgctc ctccccggtc
     2161 gattgagcct cgtcgatcgt gacggtttga ccgggcgaac acgttggtgt catcgcgagg
     2221 gccggctgtc atgcttggcg cgccggctca attcctcggc gtacgcgctt gcagcgcgtc
     2281 gtcagcaggg cacaagagga ccagagagac caaagcgaag ggacacggcg taacgcccat
     2341 gagcgcgtca tcagcgcagc c
//
LOCUS       DASUCY010000327         3533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_826331, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000327 DASUCY010000000
VERSION     DASUCY010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826331"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="VFV25_06415"
     CDS             complement(<1..845)
                     /locus_tag="VFV25_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY1392989.1"
                     /translation="MAQYVFTMNRVGKIVPPKRQILKDISLSFFPGAKIGVLGLNGSG
                     KSTLLKIMAGLDKEIEGEAVPMPDLRIGYLPQEPQLNPEQTVREAVEQGIGGVLEAKK
                     RLDEVYAEYAEPDADFDKLAAEQHELEAIIAAAGSENTDLQLELAADALRLPPWEANI
                     GVLSGGEKRRVALCRLLLSKPDMLLLDEPTNHLDAESVDWLEQFLQRFPGTVVAITHD
                     RYFLDNAAEWILELDRGMGIPWKGNYSDWLDQKEQRLEQEQKKEDARIKAMKEELKWV
                     RSNAKG"
     gene            complement(953..1963)
                     /locus_tag="VFV25_06420"
     CDS             complement(953..1963)
                     /locus_tag="VFV25_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014723772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEY1392990.1"
                     /translation="MSRPRFLPDEFTLMLVATVIAASVLPCRGIAAQVFGVITNLAIG
                     LLFFMHGAKLSREAIIAGATHWRLHLLIFAATFVMFPLLGWALRPLLEPLATPELYLG
                     LLFLCTLPATMQSSIAFTAIAHGNVPAAVCSASASSLLGIFLTPLLAGLLLSRQGGGA
                     ATSVDAVRSIVLQLLLPFIAGNVARRWIGAWVEHRKAMLGWVDRGSILLVVYTAFSAA
                     VNQGLWHQIPLPALAGLLLACALLLAAALTLTTLAARRLGFDTADEVTIVFCGSKKSL
                     ASGIPMAQVLFAGHVAGIVVLPLMLFHQLQLMVCAVLAQRYRARSLAAEAAEKPAAGA
                     AN"
     gene            2113..2676
                     /locus_tag="VFV25_06425"
     CDS             2113..2676
                     /locus_tag="VFV25_06425"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY1392991.1"
                     /translation="MAEHYRVAVLVGSLRKDSFNRRLALAVAAAAPSNFAFEHIAIGD
                     LPLYNQDHDANPGERVTALKREIEAADALLFVTPEYNRSMPGVLKNAIDIASRPYGKN
                     SFKGKPAGVIGASVGALGTAMSQQHLRNVLAYLDVPTLGQPEAFFQLSDSLIDAQGKL
                     AKEDTQKFLQHWVDTYVAWVRIHKPKG"
     gene            2885..>3533
                     /locus_tag="VFV25_06430"
     CDS             2885..>3533
                     /locus_tag="VFV25_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY1392992.1"
                     /translation="MSFDSLGLAEPLLRAVAEQGYTAPTPIQAQAIPAVLSGGDLLGG
                     AQTGTGKTAGFTLPLLHRLMAKPAKRDAKGRLPVRALILTPTRELAAQVEESVRNYGK
                     YSKLSSMAMFGGVGMQPQIDKLKRGVDILVATPGRLLDHHQQGTLDLSQIEIFVLDEA
                     DRMLDMGFIHDIKKVLAVLPAHKQSLLFSATFSDEIKALADRLLNKPALIEVARRN"
BASE COUNT          598 a         1222 c         1148 g          565 t
ORIGIN      
        1 cccttggcgt tggagcgcac ccacttcagc tcctccttca tggccttgat gcgggcgtct
       61 tctttctttt gctcctgctc caggcgctgc tccttctggt ccagccaatc ggagtagttg
      121 cccttccagg ggatgcccat gccgcggtcg agctcgagga tccactcggc cgcgttgtcg
      181 aggaagtagc ggtcgtgggt gattgccacc acggtgccgg ggaagcgctg caggaactgc
      241 tccagccagt ccaccgattc ggcgtcgagg tggttggtcg gctcgtcgag cagcagcatg
      301 tcgggcttgc tgagcaagag gcggcacagc gcgacgcggc gcttctcgcc gcctgacagc
      361 acgccgatat tggcctccca tggcggcagg cgcagcgcgt cggcggccag ttccagctgc
      421 aggtcggtgt tctcgctgcc tgccgcggcg atgatggcct ccagctcgtg ctgctcggcg
      481 gccagcttgt cgaagtcggc atcgggctcg gcgtattccg catacacctc gtccaggcgc
      541 ttcttggcct ccagcacacc gccgatgccc tgctccaccg cctcgcgcac cgtctgctcg
      601 ggattgagct gcggctcctg cggcaggtag ccaatccgga ggtcgggcat gggcacggcc
      661 tcgccctcga tctccttgtc gaggccggcc atgatcttga gcagcgtcga cttgcccgag
      721 ccgttcaggc ccagcacgcc gatcttggcg ccggggaaga aggacagcga gatgtccttg
      781 aggatctgtc gcttcggcgg cacgatcttg ccgacgcggt tcatggtgaa gacgtactga
      841 gccatggctg tcctgtgatt tctctgcagc tcgcgccgca gtgcgcggcg ccaaaagcgt
      901 ctattgtcgc tgcttcgcgt gaccggcccg ggcctgcccc aggtggtgcg tgtcagttcg
      961 cggcgcctgc cgccggcttt tcagccgctt cggcagccag cgagcgggcc cggtagcgct
     1021 gggccagcac tgcgcagacc atcagctgca gctggtggaa gagcatcagc ggcagcacca
     1081 cgatcccggc cacgtggccg gcgaacagca cttgcgccat cggtatgccg ctggccaggc
     1141 tcttcttgga gccgcagaag acgatggtga cttcatccgc ggtgtcgaag cccaggcggc
     1201 gggcggccag tgtggtgagc gtcagcgccg ccgccagcag cagggcgcag gcgagcagca
     1261 gcccggccag ggccggcagc gggatctggt gccacagccc ctggttcacc gccgcgctga
     1321 aggcggtgta gacgacgagc aggatggagc cgcggtccac ccaacccagc atcgccttgc
     1381 gatgctcgac ccacgcgcct atccagcgcc gcgccacatt gccagcgatg aagggcagca
     1441 gcagctgcag cacgatggag cgcaccgcat ccacggaagt cgcggcgccc ccgccttggc
     1501 gcgacagcag caggcccgcg agcagcggcg tcaagaagat gcccagcagg ctcgatgccg
     1561 aggcgctgca caccgccgcc ggcacattgc cgtgcgcgat cgcggtgaag gcaatggacg
     1621 actgcatcgt ggccggcagc gtgcacagga agagcaggcc gaggtaaagc tcgggcgtcg
     1681 ccagcggctc cagcaggggg cgcaaggccc agcccagcaa cgggaacatc acgaaggtcg
     1741 ccgcgaagat cagcagatgc agtcgccaat gcgtcgcccc tgcgatgatg gcctcgcgcg
     1801 agagcttggc cccatgcatg aagaagagca ggccgatggc caggttagtg atgacgccga
     1861 acacctgggc cgcgatgcca cggcagggca gcacgctggc ggcaatgact gtcgccacca
     1921 gcatcagcgt gaattcgtca ggaaggaagc gcggacggga catgggcggc gggagtgggg
     1981 ccgctatgcg gcgaggccaa gcttacggca tggccctgca cccatagccc tgcaccatag
     2041 ccttacgtca tgcaggcgtt catcgccggt cgtaaagtgg ccgccccacc actacgcgta
     2101 gagaggtacc ccatggccga acactatcgc gtcgcagtgc tcgtcggcag cctgcgcaag
     2161 gactctttca accgccgtct cgcccttgcc gtggccgcgg cggcgccgtc gaacttcgcc
     2221 ttcgagcaca tcgctatcgg cgacctgccg ctgtacaacc aggatcacga tgccaacccg
     2281 ggcgagcgcg tgaccgccct caagcgcgaa atcgaggctg ccgacgccct gctcttcgtc
     2341 acgccggaat acaaccgctc gatgcccggc gtgctgaaga acgccatcga catcgcttcg
     2401 cggccctatg gcaagaactc cttcaagggc aagccggccg gcgtgatcgg cgcctcggtc
     2461 ggcgccctcg gcacggcgat gtcgcagcag catctgcgca atgtgctggc ctatctggac
     2521 gtgcccacgc tgggccagcc cgaggccttc ttccagctca gcgacagcct gatcgatgct
     2581 cagggcaagc tcgccaagga ggatacgcag aaatttctgc agcactgggt cgatacctat
     2641 gtggcttggg tgaggatcca caagcccaag ggttgagcgg cgtagcggcg cgccgctacg
     2701 ccgccgagat tgcgtctcga gcatgcttgg cgctcgccga gtacaatgtg gccatcagcg
     2761 cggtcccagt cgccgcgctg atgtcatttc cgaccgggcc tccttgcgcc cgccgcaaca
     2821 ccccctctgc ctgcgactgg cgccccatat ggcggcgaca ggccgaccgc tttcttagcc
     2881 acccatgtcc ttcgattctc tcggcctggc cgagcccttg ctgcgcgccg ttgccgaaca
     2941 gggctacacc gcgcccacgc cgatccaggc acaagccatt cccgccgtgc tgtccggcgg
     3001 cgacctgctg ggcggcgccc agaccggcac cggcaagacc gccggcttca cgctgcccct
     3061 gctgcatcgc ctgatggcca agccggccaa gcgcgacgcc aagggccgcc tgcccgtacg
     3121 cgcgctgatt ctcacgccga cccgcgagct cgcggcccag gtcgaggaga gcgtgcgcaa
     3181 ctacggcaag tacagcaagc tcagcagcat ggccatgttc ggcggcgtgg gcatgcagcc
     3241 gcagatcgac aagctcaagc gcggcgtcga catcctggtc gcgacgccgg ggcgcctgct
     3301 cgatcatcac cagcaaggca cgctggatct ctcgcagatc gagatcttcg tgctcgacga
     3361 agccgaccgc atgctggaca tgggcttcat ccacgacatc aagaaagtgc tcgcggtgct
     3421 gccggcgcac aagcagagcc tgctgttctc ggccaccttc agcgacgaga tcaaggccct
     3481 ggccgaccgc ctgctcaaca agccggcgct gatcgaggtg gcgcgccgca acc
//
LOCUS       DASUCY010000328         2922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_521330, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000328 DASUCY010000000
VERSION     DASUCY010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521330"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1894)
                     /locus_tag="VFV25_06435"
     CDS             complement(<1..1894)
                     /locus_tag="VFV25_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY1392993.1"
                     /translation="MNAKLGISGRIAGFFQAAQITPLLALVALLLGIFAVAVTPREEE
                     PQINVTMANVLVPFPGASVPDVEQMVATPAEQVLSQIAGIEHVMSISRPGLAVITVQF
                     KVGVPRTEALVRLYDTVNSNADWLPKGLGVGEPLIKPKGIDDVPIVTLTLYSKDAAST
                     AFDLERVAHSVEADLKRVPGTREVTTLGGPGRAVLVEIDPARLAGAGVTVADLRRALQ
                     SANLGAPVGELLGDNRSVALEAGPFLRDAREAGALVVGVHGDKPVFLQDVATLHDGAP
                     PAQRYVWHGIAGAQGGEYPAVTITITKKPGENAIDVAKAVTRRIDSLRNTLIPQDVEV
                     AETRNYGATANDKAMKLIEKLLFATASVVALVFIALGRREAAIVGTAVILTLTVTLFA
                     SWAWGFTLNRVSLFALIFSIGILVDDAIVVVENIHRHQALHPEKKLAELIPGAVDEVG
                     GPTILATLTVIAALLPMAFVSGLMGPYMSPIPINASMGMLLSLAIAFMVTPWLARLWM
                     KPHAAHGAAPKGLAAWLGPLFERIFRPLLDEHRGTRNRALLGAGVAGLIAVSLALPAL
                     GLVVLKMLPFDNKSEFQVVVDMPAGTPVERTAAVLRELGAYLARQPEVTDYQAYAGTA
                     APINFNG"
     gene            complement(1907..>2922)
                     /locus_tag="VFV25_06440"
     CDS             complement(1907..>2922)
                     /locus_tag="VFV25_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY1392994.1"
                     /translation="WLLTIGTALAADGQDSPSPLSSAPVTRGAAAAGEAYDGVVQALR
                     QTVLAAQVPGAIVALEVKAGDRVKAGQLLLRLDARAAEQQAGAAAAQVQAARATQEAA
                     TKEFERQKLLYQKNYISQAALERAEAQYKAAQAEASAQLASAGAARTQSGFYAVKAPY
                     DGIVADVTVVLGDMAMPGRPLLTVYDPGALRVSARVPQSAAAKLKPGQAMQAEVPGAV
                     AGLVPPVRTQWLPAVDPATHTVELRLDLPASFSGLAPGMFARAWLPAAGGEADTRLFM
                     PARAVIRRAELTAVYVIGVDGRPLLRQVRLGRTEGDRVEVLTGVSAGERLALDPQAAA
                     RVR"
BASE COUNT          450 a         1029 c         1005 g          438 t
ORIGIN      
        1 ggccgttgaa attgatcggc gccgctgtgc cggcataggc ctggtagtcg gtgacctccg
       61 gctggcgtgc taagtaggca cccagctcgc gcagcacggc cgcggtccgt tcaaccggcg
      121 tgccggccgg catgtcgacc accacctgga actccgactt gttgtcgaag ggcagcatct
      181 tcagcaccac gagtcccagc gccggcagcg ccaatgacac ggcgatcaag ccggccaccc
      241 ctgcgcccag cagcgcgcgg ttgcgggtgc cgcgatgctc atcgagcagc gggcggaaga
      301 tgcgctcgaa cagcgggccg agccaggcgg ccaggccctt gggtgctgcg ccatgtgctg
      361 cgtgcggctt catccacagc cgcgcaagcc agggcgttac catgaaggcg attgccagcg
      421 acagcagcat gcccatgctg gcgttgatcg ggatcggact catgtagggg cccatcaggc
      481 ccgacacgaa ggccatcggc agcagcgcgg cgatcaccgt cagcgtggcg aggatggtgg
      541 ggccgcccac ctcgtcgacg gcgcccggga tcaactcggc cagcttcttt tccgggtgca
      601 aggcctggtg gcgatggatg ttctcgacga cgacgatggc atcgtccacc aggatgccga
      661 tcgagaagat cagcgcgaac agcgacacgc ggttgagcgt gaagccccag gcccaggagg
      721 cgaacagcgt caccgtgagc gtgaggatca ccgcggtgcc gacgatcgcc gcctcgcgcc
      781 gtcccagcgc gatgaacacc agcgccacga ccgaggcagt ggcgaacagc agcttctcga
      841 tgagcttcat cgccttgtcg ttggccgtcg caccgtagtt gcgggtctcg gccacttcca
      901 cgtcttgcgg gatcagggtg ttgcgcagcg agtcgatacg gcgcgtcacg gccttggcca
      961 cgtcgatggc gttctcgccg ggcttcttgg tgatcgtgat ggtgacggcg gggtattcgc
     1021 cgccctgggc accggcgatg ccgtgccaca catagcgctg cgccggcggc gcgccgtcgt
     1081 gcagcgtcgc cacgtcctgc aggaacaccg gcttgtcgcc gtgcacgccg accaccagcg
     1141 cgccggcctc gcgcgcgtcg cgcaggaagg gcccggcctc gagtgcgacc gagcggttgt
     1201 cgcccagtag ttcgcccacc ggcgcgccga gattggccga ctgcaatgcg cggcgcaggt
     1261 cggccaccgt gacgccggcg ccggccagcc gcgccgggtc gatctctacc agcactgcgc
     1321 gcccaggccc gccgagcgtg gtcacttctc gcgttcccgg cacgcgcttc aggtccgcct
     1381 ccacactgtg agctacccgc tcgagatcga aggcagtgct cgcagcgtcc tttgaataga
     1441 gggtgagggt gacgatgggc acgtcgtcga tgcccttggg cttgatcagc ggctcgccca
     1501 cgcccaggcc cttgggcaac cagtcggcat tggaattcac cgtgtcgtag agccgcacca
     1561 gcgcctcggt gcgcggcacg ccgaccttga actgcacggt gatcaccgcc agcccgggcc
     1621 gcgagatgga cattacgtgc tcgatgccgg cgatctgcga gagcacctgc tcggccggtg
     1681 tcgccaccat ctgctcgaca tcgggtaccg acgcaccggg gaagggcacc agcacgttgg
     1741 ccatcgtaac gttgatctgc ggctcctcct cgcgcggcgt caccgccaca gcgaagatgc
     1801 ctagcagcag cgccaccagc gccagcagcg gtgtgatttg cgcagcctgg aagaaaccgg
     1861 caatgcggcc ggagatgccg agcttggcgt tcatgctggc cacccctcag cgcacgcgcg
     1921 cggcggcctg cggatcgagc gccaagcgct cgccggccga cacgccggtc agaacctcga
     1981 cccggtcgcc ttcggtgcgg cccagccgca cctggcgcag caagggacga ccgtcgacgc
     2041 cgatgacata gacagccgtg agttcggcgc gcctgatcac cgcacgggcc ggcatgaaga
     2101 gacgcgtgtc ggcctcgccg ccggctgccg gcagccaggc gcgcgcgaac atgccgggcg
     2161 cgaggccgga aaagctggcc ggcaggtcca gccgcagctc caccgtgtgg gtcgcgggat
     2221 cgacggcggg cagccattgg gtcctgacgg gcggcaccag tcccgcgacg gctccaggca
     2281 cctcggcctg catggcctgg cccggcttca gcttcgcggc cgcgctttgc ggcacccgtg
     2341 cgctgacgcg cagcgcgccg gggtcgtaga cagtgagcag cggacgccct ggcattgcca
     2401 tatcgcccag caccaccgtc acgtcggcga cgatgccgtc gtagggagcc ttcaccgcgt
     2461 agaagccaga ctgggtacgc gccgcgcccg cgctggcgag ctgcgccgag gcttcggcct
     2521 gcgccgcctt gtactgcgcc tcggcccgct ccagcgcggc ctggctgatg tagttctttt
     2581 ggtagagcag cttctggcgc tcgaactcct tggtggccgc ctcctgcgtg gcgcgcgcgg
     2641 cctgcacctg tgcagcggcg gcgccggctt gctgctcggc ggcgcgtgca tccaggcgca
     2701 gaagcagctg cccggccttc acacggtcgc cggccttgac ctcgagtgcc actatggcgc
     2761 cgggaacctg cgcggccagc accgtctgcc gcagcgcctg cacgacgccg tcataagctt
     2821 cgccggcagc ggctgcgccg cgcgttacag gcgcactcga aagcgggctt ggggagtcct
     2881 gcccgtcggc ggcaagcgcg gtgccgatcg tcagcaacca ag
//
LOCUS       DASUCY010000329         2247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_135658, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000329 DASUCY010000000
VERSION     DASUCY010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135658"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VFV25_06445"
     CDS             <1..447
                     /locus_tag="VFV25_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEY1392995.1"
                     /translation="DVAFRMTDFAVRNAQRAGVASAIEFKTADALQRPAPAEAGTLML
                     NPPYGERIAPKGSRAGAQGGEASRATREGFEQGSAEFFSRLAAHWKRGYAGWTAWVLS
                     PDMKLPQAMRLKESRRVVMWNGPIECRLFRFDLVAGAMREQRGNGA"
     gene            complement(474..1301)
                     /locus_tag="VFV25_06450"
     CDS             complement(474..1301)
                     /locus_tag="VFV25_06450"
                     /inference="COORDINATES: protein motif:HMM:NF025705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4399 domain-containing protein"
                     /protein_id="HEY1392996.1"
                     /translation="MLKHHLGQKFRFATIGAAMLVAASLACAADQAPDRHPWISAPPQ
                     LKQPTTYFSNLKDGDRIETPYLLKFGLSRYGLAPIVKAVPNTGHHHLLVNRELPLDFG
                     KPLPFDEHYIHFGKGQMEKVLTFPPGTYTLRLLLADHRHIPYFVYSKPMTITVTKNHP
                     DIDPNSLVKPGVELLAPHAGARLEPPFLVQFHASGLNVGAAEIKDEGVGHFRLVAERA
                     GAAPEPIDFGDGHTEAWLRPPAGAYKLRLELVSNADPSKVMASSAPVDVHVAAAAKH"
     gene            complement(1323..1742)
                     /locus_tag="VFV25_06455"
     CDS             complement(1323..1742)
                     /locus_tag="VFV25_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HEY1392997.1"
                     /translation="MGLLDSVLGAITGQTGGAAGGGNSALLQAAIGMLANNSAGGGIA
                     GMAQQFQQAGLGHLLDSWVGQGQNLPISADQITQVLGSGQIGQIAQQLGLSHGDAAGQ
                     LAQILPQVINQLTPNGQVPQGGLGEVGELLGKVLQQR"
     gene            complement(1889..>2247)
                     /locus_tag="VFV25_06460"
     CDS             complement(1889..>2247)
                     /locus_tag="VFV25_06460"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HEY1392998.1"
                     /translation="AACEQSGRTRLPRLEPVLSLADWLRACQAADPKAAPRRHVLSLR
                     TNAATFAQVLHATLDAPPGGDPGWLFLSGPEGGLSEAEETLARAAGWVPLRLGERTLR
                     AETAPLAALAALSALF"
BASE COUNT          332 a          742 c          800 g          373 t
ORIGIN      
        1 gacgtcgcct ttcgcatgac cgacttcgcc gtgcgcaatg cgcagcgtgc aggcgtggcc
       61 tcggccatcg aattcaagac tgccgatgcg ctgcagcgcc cggcgccggc cgaagccggc
      121 acgctgatgc tgaacccgcc ctatggcgaa cgcatcgcgc cgaaaggttc gcgcgcaggc
      181 gctcagggcg gcgaggccag tcgcgccacg cgcgaaggct tcgagcaggg ctcggccgag
      241 ttcttcagcc gcctggccgc gcattggaag cgcggctatg ccggctggac cgcctgggtg
      301 ttgagcccgg acatgaagct gccgcaagcc atgcggctaa aggaaagccg ccgcgtcgtg
      361 atgtggaacg ggccgatcga atgccggctg ttccgcttcg atctcgtcgc cggcgcgatg
      421 cgcgagcagc gtggcaacgg cgcgtagtgc cggcgtgccg ggccggcgcg cgctcagtgc
      481 ttggcggctg ccgcgacgtg cacgtccaca ggcgcgctgc tggccatcac cttgctcggg
      541 tcagcattgc tgaccagctc cagcctgagc ttgtaggccc cggccggcgg ccgcagccag
      601 gcctcggtgt gcccgtcgcc gaagtcgatg ggctcgggcg cggcaccggc gcgctcggcc
      661 acgaggcgga aatggccgac gccctcgtcc ttgatttcgg cggcaccgac attgaggccc
      721 gaggcgtgga actgcaccag gaagggcggc tcgagccgcg cgccggcatg cggcgccagc
      781 agctccacgc cgggcttgac caggctattg gggtcgatgt ccgggtggtt cttcgtcacc
      841 gtgatggtca tcggcttgct atagacgaaa taggggatgt gtcggtggtc ggccagcagc
      901 aggcgcagcg tataagtgcc cggcgggaag gtcagcacct tttccatctg gcccttgccg
      961 aagtggatgt agtgctcgtc gaagggcagc ggcttgccga agtccagcgg cagctcgcgg
     1021 ttgaccagca gatggtggtg gcccgtgttg ggcaccgcct tcacgatggg cgccaagccg
     1081 tagcgcgaga ggccgaactt cagcagatag ggcgtctcga tgcggtcgcc gtctttgaga
     1141 ttgctgaagt aggtggtggg ctgcttcagt tgcggcggcg cgctgatcca ggggtggcga
     1201 tcgggcgcct ggtctgcagc gcaggccagg ctggccgcga ccagcatggc agcccctatc
     1261 gtcgcgaacc ggaacttctg accaaggtgg tgcttgagca tcggcggcct atcggtggcg
     1321 tgtcagcgtt gctgcaggac cttgcccagc agctcgccca cttcgcccaa gccgccttgc
     1381 ggcacctggc cattgggcgt gagctggttg atgacctggg gcaggatctg cgccagctgc
     1441 ccggccgcgt cgccatgcga gaggcccagc tgctgcgcga tctggccgat ctggcccgag
     1501 cccagcacct gcgtgatctg gtccgccgag atcggcaggt tctgtccctg gccgacccag
     1561 gaatcgagca gatgccccag gcccgcctgc tggaactgct gggccattcc cgcaatgccg
     1621 ccgccggccg agttgttggc cagcatgccg atggccgcct gcagcagcgc gctgttgccg
     1681 ccgcccgccg cgccacccgt ctggcccgtg atcgcgccca gtaccgaatc caatagtccc
     1741 atcgtgtctc tccttgcggg gacgaacccc ctggcgggcg attgtggcga ggacgggtgt
     1801 cagcggcaag gcagccgatc gacgagtggg ttgctacaaa ttctttgtcg tgaatggcag
     1861 ataaattcgg gtaggcacct attttttatc aaaacagtgc ggaaagggca gccagtgccg
     1921 ccaagggcgc ggtctcggcc ctcagcgtgc gctcgcccag gcgcagcggc acccagccgg
     1981 cggcgcgggc cagcgtttcc tcggcttcgc tgaggccacc ctccggcccg ctgaggaaga
     2041 gccatcccgg atcgccgccc ggcggcgcat cgagggtcgc gtgcagcacc tgggcgaagg
     2101 tcgccgcgtt ggtgcgcagg ctcagcacgt ggcggcgcgg tgcggctttc gggtccgctg
     2161 cttggcaagc gcgcaaccag tcggccaggc tcagcaccgg ctccagccgc ggcagcctgg
     2221 tgcgcccgct ctgttcgcag gcggcga
//
LOCUS       DASUCY010000330         2488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_39375, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000330 DASUCY010000000
VERSION     DASUCY010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39375"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..1929
                     /locus_tag="VFV25_06465"
     CDS             154..1929
                     /locus_tag="VFV25_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEY1392999.1"
                     /translation="MKAELMDDNETLQMRPLRSGRWFRKDDLPGFVHRSTLAAAGWSR
                     EDLMTRPVIGILNTWSDINPCNLNLRALAEDVRTGILEAGGIPFEIPLMSLSENIMKP
                     SSFPFRNLLAMEVEETLRAHPFDAVVLLGGCDKTQPALLMGAASAGIPFLFLASGPAT
                     PRSATGGQLANATGVWRLVDQLRAGQTQCDFAAFERSVMGTVGHCAEMGTASSMSAIC
                     EALGVALPGSSMVPATDRRRNQLARETGRRSVKLAAEGGPRPEDILDARAFDNAITLL
                     CAVGGSTNCIIHLLALAGRVGVPLTLERFDEIGRKVPLIADVQPAGEHLTERLMTAGG
                     VPALLKALKPLLHLDARTVDGRTIGEIIESAEVLDPEVIRPLDKPVKPGETLVVVKGN
                     LAPDGAVMKACAADPKLFRHRGRAVVFEDVQSIADRIDDPALGIDENSVLILRNAGPV
                     GAAMPEWGMLPLPRRLLERGVRDMLRISDARMSGTAYGAAVLHVSPEAAVGGPLALVR
                     DGDIIELDVAERRLQLCVDPEELERRRQAWQPPVRVHARGYRRLYDQHVEQAHRGCDF
                     DFLRGADADAETLPEGLFDGWVGGW"
     gene            1933..>2488
                     /locus_tag="VFV25_06470"
     CDS             1933..>2488
                     /locus_tag="VFV25_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY1393000.1"
                     /translation="MSILYRSDAPRAGAWARYFAEHAPDLDFRVWPEAGKLEEIEYLI
                     AWQAPREFLAALPRLKVLFSSGAGVDHVDFSAVPAHIPIARMVEPGIINGMVEYVSLA
                     VLALHRDFFDYVAAKASRSWDALEVPPASSRTVGVMGMGVLGRAVLERLVAYGFRLRG
                     WNRSLRPMQGVQSFAGAEQLRPFLA"
BASE COUNT          373 a          872 c          849 g          394 t
ORIGIN      
        1 ggcgaggccg ccgccaaccc ctggcgcgac gactcttggc cagccattcg aggccacttc
       61 ggcccgccct ggttcttgcc cgccatcggc gtctattacc ggctcaagga caagctggct
      121 tgattccatt catttcaacc agcgacacat tccgtgaagg ctgaactcat ggacgacaac
      181 gagaccctgc agatgcggcc gctgcgaagc gggcgctggt tccgcaagga cgacctgcct
      241 ggcttcgtgc accgctctac gttggcagcg gccggatgga gccgtgagga cctgatgacg
      301 cgacccgtca tcggcatcct gaacacctgg tcggacatca acccctgcaa cctcaatttg
      361 cgcgcgttgg cggaagacgt gcgcacgggc atcctggagg cgggcggcat cccgttcgag
      421 attccgctga tgtcgctgag cgagaacatc atgaagccca gctcgttccc gttccgcaac
      481 ctgctggcga tggaggtgga ggagacactc cgcgcccatc ctttcgatgc cgtggtgctg
      541 ctcggaggct gcgacaagac gcagccggcc ctgctgatgg gcgcggccag cgccgggatc
      601 ccgttcctct ttctggccag cggcccggcg acgccacggt cggcgacggg tggccagctg
      661 gcgaacgcca ccggcgtctg gcgcctggtc gaccagctgc gcgccggcca gacgcaatgc
      721 gatttcgcgg ccttcgagcg cagcgtgatg ggcaccgtgg gccactgtgc cgagatgggc
      781 accgcgtcgt cgatgtcggc catctgcgaa gcgctgggcg tggccctgcc cggcagcagc
      841 atggtgccgg cgacagaccg ccgccgtaac cagctggccc gcgagaccgg ccgccgcagc
      901 gtcaaactcg ccgccgaagg cggcccacgc cccgaggaca tcctcgatgc gcgcgccttc
      961 gacaacgcca tcaccctgct gtgcgcggtg ggcggctcca ccaactgcat catccatctg
     1021 ctggcactgg ccggccgcgt cggcgtgccc ttgacccttg agcgcttcga cgagatcggg
     1081 cgcaaggttc cgctgattgc cgacgtgcag cctgccggcg aacacctgac cgagcggctg
     1141 atgaccgccg gcggcgttcc cgcgttgctc aaggcactca agcccttgct ccacctggac
     1201 gcgcgcacgg tcgacgggcg caccatcggc gagatcatcg agagcgccga ggtgctggac
     1261 ccggaggtca tccggccact ggacaagccc gtgaagcccg gcgaaacgct ggtcgtggtg
     1321 aagggcaacc tcgcgcccga cggcgccgtg atgaaggcct gcgccgccga ccccaagctg
     1381 ttccgccatc gcggccgcgc ggtggtgttc gaggacgtgc agagcatcgc cgaccgcatc
     1441 gacgacccgg ccctggggat cgacgagaac agcgtgctga tcctgcgcaa cgccggcccg
     1501 gtgggcgcgg ccatgcccga gtggggcatg ctgccactgc cgcgccggct gctggagcgt
     1561 ggcgtgcgcg acatgttgcg catttccgac gcacgaatga gcggaacggc ctacggcgcg
     1621 gccgtgctgc atgtctcacc cgaagccgcg gtcggcggcc cgctcgcgct ggtgcgcgac
     1681 ggcgacatca tcgagctcga tgtcgccgaa cgccggctgc aattgtgcgt ggaccccgaa
     1741 gagctggaac gacggcgcca ggcctggcag ccgccggtgc gggtgcatgc gcgcggttac
     1801 cgccggctct atgaccaaca cgtggagcag gcgcatcgcg gctgcgattt cgacttcctg
     1861 cgcggcgccg acgccgacgc cgagaccctg cccgaagggc tgttcgacgg ctgggtggga
     1921 ggctggtagg ccatgagcat tctctaccga tcggacgcgc cgcgcgccgg agcctgggcg
     1981 cgctacttcg ccgagcacgc ccccgacctc gatttccgcg tctggcccga agccggcaag
     2041 ctcgaggaga tcgaatacct gatcgcctgg caggcgccgc gggagttcct cgccgccttg
     2101 ccccggctca aagtgctgtt ctcctctggc gccggggtcg accatgtcga cttctcggcc
     2161 gtgccggctc acattcccat cgcgcgcatg gtggagcccg gcatcatcaa cggcatggtc
     2221 gagtacgtga gcctcgccgt gctggccctg catcgggact tcttcgacta cgtggctgcg
     2281 aaggcatcgc gcagctggga cgcgctggag gtgccgcccg cctcaagccg caccgtcggc
     2341 gtcatgggca tgggcgtgct cggacgcgcg gtgctggagc gtctggtggc ctatgggttc
     2401 cggctgcgtg gctggaaccg ctcgctgcgc cccatgcagg gcgtgcagag tttcgccggc
     2461 gccgagcagc tgcggccctt cctcgccg
//
LOCUS       DASUCY010000331         6774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_248271, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000331 DASUCY010000000
VERSION     DASUCY010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 6774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6774
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248271"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VFV25_06475"
     CDS             <1..269
                     /locus_tag="VFV25_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393001.1"
                     /translation="GHLFWLALAPEGTRSLTEGWRSGFYHVACQARVPLGVAAFDYGS
                     KCIDVDYFLQLSGDARADIAAVAAHLSRVRGCRPELAAPIRLKA"
     gene            276..1220
                     /gene="hemF"
                     /locus_tag="VFV25_06480"
     CDS             276..1220
                     /gene="hemF"
                     /locus_tag="VFV25_06480"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018911753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HEY1393002.1"
                     /translation="MTEANRAIVFRDYLLDLQQRIVAALEAEGGEAFLSDAWTRPAGG
                     KLEGDGHSRLIEGGQLLERGGCNFSHVKGAALPPSATQHRPELAGAPFEALGVSLVFH
                     PRNPYVPTVHMNVRMLTACKPGGESVSWFGGGMDLTPYYGYEEDAAHFHLRCLDALAP
                     HGIDKYPRFKKWCDEYFFLKHRNEPRGIGGIFYDDFSELGFDGGFAMTRSVGDAFLAA
                     YLPIVQRRKGQPYGEREREFQAYRRGRYVEFNLVWDRGTLFGLQSGGRTESILMSMPP
                     VASWRYDWRPEPGTPEARLYGDFLHPRRWVEPGAPAEA"
     gene            1230..1829
                     /gene="nadD"
                     /locus_tag="VFV25_06485"
     CDS             1230..1829
                     /gene="nadD"
                     /locus_tag="VFV25_06485"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEY1393003.1"
                     /translation="MRIGFFGGSFDPVHQAHLALARLALDHLKLDRLLWVPVGRPWQK
                     QHELAADEHRVAMLKLAIAGELRYALDTMELDRAGPSYTLDSVRELRAREPRAEIFLI
                     IGQDQYAGLPSWHGWRDLLPLVTLAVAGRNAEAPLPAAALAAVPHRVEALPLPAMAVS
                     STEIRERLARGEAAAALVPALVPAAVARYIDQHRLYAGA"
     gene            1845..2552
                     /gene="rsfS"
                     /locus_tag="VFV25_06490"
     CDS             1845..2552
                     /gene="rsfS"
                     /locus_tag="VFV25_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEY1393004.1"
                     /translation="MDIRKLQRAIVDGLEDVKAHDIAVFNTEHLSPLFERVIVASGTS
                     NRQTKALAASVRDAVKTSGFPVMRTEGEDNGEWIIVDCGAAVAHIMQPAIREYYHLEE
                     IWGDKPVKMKLASQARGLPKASHANGGDDGAATRKASASRGATAPAKKTAAKKAAAKK
                     PAAKPATGRTAAGKAATGKKASATGKSSSGARARKPAAKVAKTIVVKSGRPGTAARPA
                     AKATAAKKKAPARKPVR"
     gene            2562..3032
                     /gene="rlmH"
                     /locus_tag="VFV25_06495"
     CDS             2562..3032
                     /gene="rlmH"
                     /locus_tag="VFV25_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEY1393005.1"
                     /translation="MKLLLVAVGQRVPAWAEAACEDYLKRFPPELKLELKALKTEPRS
                     SGKPVEALMEAERSRIAAAIPKGARLVVLDEHGSRVTTQQLAARLDAWMLDGRDVALV
                     IGGPDGLAPELKHAADETLRLSDLTLPHAMARVILAEALYRAWSLRAGHPYHRE"
     gene            3050..3673
                     /locus_tag="VFV25_06500"
     CDS             3050..3673
                     /locus_tag="VFV25_06500"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEY1393006.1"
                     /translation="MTPRFIYLASQSPRRRQLLTQIGVEHELLLPDEDEDAEALEAVL
                     PAELPEVYVERVTRAKLQAARARWLRRQTDGLPPAPILCADTTVALGAQILGKPRDDD
                     DAAAMLARLSGRTHRVITAVAVAHAAGGAGEAYAASVSHVRLRALGADEIERYVASGE
                     PRGKAGAYAIQSQAGAWIEHIEGSYSGIMGLPLYETAELLRPLGWQF"
     gene            3747..5216
                     /gene="rng"
                     /locus_tag="VFV25_06505"
     CDS             3747..5216
                     /gene="rng"
                     /locus_tag="VFV25_06505"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HEY1393007.1"
                     /translation="MQDILINWSPQETRVAVVEGGAVQELHVERTLERGLVGNIYTGR
                     VARVLPGMQSAFIDIGLERAAFLHVADVHHNGARGHGNEANGAPPTPIERLVFEGQTL
                     MVQVVKDPIGSKGARLSTQISIAGRLLVFLPQDDHIGVSQKIGSQELREQLRLRLQAL
                     AGEEGGGFILRTNAEDASDAELADDIAYLRKTWAAVRERSFKVPPGTLLHEDLNLVQR
                     VLRDLANDQTQTIRIDSRMQYETLRGFGEEYTPTAVSRLEHYKGERPIFDLYNIDQEI
                     ERALARRVDLKSGGYLIIDQTEALTTVDVNTGGYVGARNFDDTIFKTNLEAALAIARQ
                     LRLRNLGGIIIIDFIDMVREEHQEAVLAELRKQLARDRTKITVSGFTQLGLVEMTRKR
                     TRESLAHMLCEPCPTCEGRGQVKTARSVCYDILREILREARQFNPKEFRVVASAGVVE
                     MLLDEESQHLAGLSDFIGKPISLQTEPSMSPEQYDIVLL"
     gene            5226..6068
                     /locus_tag="VFV25_06510"
     CDS             5226..6068
                     /locus_tag="VFV25_06510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY1393008.1"
                     /translation="MQFSIIIPSWNNLAHLQLVLRSIREHSAYAHQVIVHVNDGSDGS
                     REWVRSQGLDATYSDANIGICYAVNEAAMLARHDYIVYMNDDMYCCPGWDSALARRIA
                     TMPSDLFMLSGTMVEPRDSGNPCVAVGPYGDAVDNFQEQSLLAALPRLKRADWYGATW
                     PPTLVHRRWWFRCGGYSTELSPGMSSDNDFSMKLWFAGCRHFIGVGDSLVYHFQQKST
                     GKVVKNDGRRQFLAKWGMTQSTFDRHYLRRGEPLQGDALPEPQDTPALRWALRKAALK
                     RRFA"
     gene            complement(6069..>6774)
                     /locus_tag="VFV25_06515"
     CDS             complement(6069..>6774)
                     /locus_tag="VFV25_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY1393009.1"
                     /translation="FVAAGDDVSRPQRCSRVVEAWLASGRKLDLIAAPLQDIDAEGRV
                     QAIITPSDLGAYRNLRDWANKLPHVVGAAQAWTRRLLECFGPLPEGVVAEDLIMVFRA
                     IGSGGAITLAEPLVQYRRGGISRRVRSLSAQDVIMRLKKNNRHALVELPLLLRDAELM
                     GALADVQPVIAPQLVRERYIHDLFHAATLRGRLRCFWAAADVPLGARCRLLVYAACPG
                     LLAPSFWLKRRFARRD"
BASE COUNT         1140 a         2306 c         2313 g         1015 t
ORIGIN      
        1 aagggcactt gttctggctc gcgctggcgc ccgagggcac gcgcagcctg accgagggct
       61 ggcgctccgg cttctaccat gtggcctgcc aggcgcgcgt gccgctgggc gtggccgcct
      121 tcgactatgg cagcaagtgc atcgatgtcg attacttcct gcagctcagc ggcgatgcgc
      181 gagccgacat cgcggccgtg gcggcgcatc tgtccagggt gcgaggctgc aggcccgagc
      241 tggcagcgcc catacgactc aaggcttgag cgagcatgac agaagcaaat cgcgccatag
      301 tctttcgcga ctacctgctg gacctgcagc agcgcatcgt cgcagcgctg gaggccgagg
      361 gtggtgaagc cttcctgagc gatgcctgga cccggccggc cggcggcaag ctcgaaggcg
      421 atggccactc gcgcctgatc gagggcgggc agctgctgga gcgcggcggc tgcaacttct
      481 cgcatgtgaa gggcgcggcg ctgccgcctt cggcgaccca gcaccggccc gagctggcgg
      541 gggcgccttt cgaggccttg ggcgtgtcgc tggtctttca cccgcgcaac ccctatgtgc
      601 cgaccgtgca catgaatgtg cgcatgctga cggcgtgcaa gcccggcggc gagagcgtga
      661 gctggttcgg cggcggcatg gatctgacgc cctactacgg ctacgaggaa gacgcggcgc
      721 atttccacct gcgctgtctc gatgccttgg cgccgcatgg catcgacaag tacccgcgct
      781 tcaagaaatg gtgcgacgag tacttcttcc tgaagcaccg caacgagccg cgcggcatcg
      841 gcggcatctt ctatgacgat ttctccgagc tcggcttcga tggcggcttt gcgatgacgc
      901 gctcggtcgg cgatgccttc ctggctgcgt atctgcccat cgtgcagcgg cgcaagggcc
      961 agccctatgg cgagcgcgaa cgcgagttcc aggcctatcg ccgcggccgc tatgtcgagt
     1021 tcaatctcgt gtgggaccgc ggcacgctgt tcggcctgca gtccggcggg cgaaccgaat
     1081 ccatcctgat gtccatgccg ccggtcgcta gctggcgcta cgactggcgt cccgagcccg
     1141 gtacgcccga ggccaggctc tacggcgact tcctgcaccc gcgccgctgg gtcgagcccg
     1201 gcgcacccgc tgaagcctga gcaacgccta tgcgcatcgg cttcttcggc ggcagcttcg
     1261 acccggtgca ccaggcgcat ctggcattgg cccgtctggc actcgatcac ttgaagctcg
     1321 atcgcctgct ctgggtgcct gttggccggc cctggcagaa gcagcacgaa ctcgcggccg
     1381 acgagcatcg cgtcgcgatg ctgaagctcg cgatcgccgg ggagctgcgc tatgcgctgg
     1441 acacgatgga gctggaccgt gccggcccga gctacacgct ggacagcgtg cgcgaactgc
     1501 gagcgcgcga gccgcgtgct gaaatcttcc tcatcatcgg ccaggaccag tatgccgggc
     1561 tgccgagctg gcacggctgg cgcgacttgc tgccgctggt gaccctggcg gtcgcggggc
     1621 gcaacgccga ggcgccgctg cccgcggcgg cgcttgcggc cgtgccgcat cgcgtcgaag
     1681 cgctgccgct gccggccatg gcggtctcgt ccaccgagat acgcgagcgg ctggcgcgtg
     1741 gcgaagcggc cgcagccctc gttcccgcgc tggttcccgc cgcggtcgcg cgctatattg
     1801 atcagcaccg gctctatgcc ggcgcataac ctccacggag ctgaatggac atccgcaaac
     1861 tgcagcgcgc catcgtcgat ggcctggaag acgtcaaggc ccatgacatc gcggtcttca
     1921 ataccgagca tctgtcgccg ctcttcgagc gcgtgatcgt cgcctcgggt acctccaacc
     1981 gacagaccaa ggcgctcgca gccagcgtgc gcgatgcggt caagaccagc ggcttcccgg
     2041 tgatgcgcac cgaaggcgag gacaacggcg aatggatcat cgtggactgc ggcgccgccg
     2101 tggcccacat catgcagccg gcgatacgcg agtactacca cctggaagaa atctggggcg
     2161 acaagccggt caagatgaag ctcgccagcc aggccagggg cttgcccaag gcctcgcatg
     2221 ccaatggcgg cgatgacggc gcggcgacgc gcaaggcatc agccagtcgc ggcgctacgg
     2281 cgccggcgaa gaagacagcg gccaagaaag ccgcggccaa gaaacccgcc gcgaagccgg
     2341 cgacgggcag gacggccgcc ggcaaggcag cgaccggcaa gaaggcctcc gccaccggca
     2401 agagttcaag cggagcccgc gccaggaagc cggccgcgaa ggtcgccaag accatcgtcg
     2461 tgaagtctgg ccggcccggc acggcggcca ggcctgcagc caaggcgact gccgccaaga
     2521 agaaggcgcc cgcacgcaag ccggtccgct gagcggcggc catgaagctg ttgctcgtgg
     2581 ccgtgggcca gcgcgtgccg gcctgggccg aggcggcctg cgaggactat ctcaagcgct
     2641 ttccgcccga gctgaagctg gaactcaagg ccctgaagac tgagccgcgc agcagcggca
     2701 aaccggtcga ggccttgatg gaggccgagc gctcgcgcat cgcggcggcc atccccaagg
     2761 gcgcgcgtct ggtcgtgctg gatgagcatg gcagccgcgt cacgacccag cagctggcgg
     2821 cgcggctcga tgcctggatg ctggacggcc gcgacgtggc cctggtcatc ggcgggccgg
     2881 acggcctcgc gcccgagctg aagcacgcag ccgacgaaac gctgcgcctg tcggacctga
     2941 cgctgccgca tgcgatggcg cgggtgatcc tcgccgaggc cttgtaccgc gcctggtcgc
     3001 tgcgcgccgg ccacccctat caccgcgaat gacggaaaag taggacgcca tgacgccgcg
     3061 cttcatctac ctcgcctcgc aaagccccag gcggcgccaa ttgctgacgc agattggcgt
     3121 ggagcacgag ctgctgctgc ccgacgagga cgaggatgcc gaggcgctgg aggcggtgct
     3181 gccggccgag ttgcctgagg tctatgttga gcgcgtgacg cgcgccaagc tgcaggcggc
     3241 tcgggcacgc tggctgcgac ggcaaacgga tggcctgcca ccggcgccca tcctttgcgc
     3301 cgacacgacg gtcgcgctgg gcgcacagat cctcggcaag ccgcgcgacg atgacgatgc
     3361 ggcggccatg ctggcgcgtt tgtccgggcg cacgcatcgc gtcattaccg ctgtggccgt
     3421 ggcccatgcg gcgggtgggg cgggagaggc ctatgccgcc agtgtgtcgc atgtgcgctt
     3481 gcgagcattg ggcgccgacg agatcgagcg ctacgtggcc agcggcgaac cgcggggcaa
     3541 ggcaggcgct tacgcgatcc agagccaggc gggggcgtgg atcgagcaca tcgagggcag
     3601 ctactcgggc atcatggggc tgccgctgta cgagacggct gagttgctgc gcccgctcgg
     3661 ttggcagttc tagccgtccg tgatgaggca ccccgctcac cgtacccggg gagcggggtg
     3721 ctccattcct ccagcacaat cgccccatgc aagacatcct gatcaattgg tcgccgcagg
     3781 aaacgcgcgt cgccgtcgtc gaaggcggcg cggtgcagga actgcacgtc gagcgcacgc
     3841 tggagcgcgg cctggtcggc aacatctaca ccggccgtgt ggcccgcgtg ctgccgggca
     3901 tgcaatcggc cttcatcgac atcggcctgg agcgtgcagc cttcctgcac gtggccgacg
     3961 tgcatcacaa cggcgcccgc gggcatggca acgaagccaa cggcgcgccg ccgaccccga
     4021 tcgaaaggct ggtgttcgaa ggccagacgc tgatggtgca ggtcgtgaag gatccgatcg
     4081 gcagcaaggg cgcgcgtttg tcgacccaga tcagcatcgc gggtcggctg ctcgtcttcc
     4141 tgccgcagga cgaccacatc ggcgtttcgc aaaagatagg ctcgcaggag ctgcgcgagc
     4201 agctgcgcct gcgcctgcag gccctggcgg gcgaggaggg cggcggcttc atcctgcgca
     4261 ccaatgccga ggacgcgagc gatgccgagc tggccgacga cattgcctac ctgcgcaaga
     4321 cctgggccgc ggtgcgcgag cgcagcttca aggtgccgcc cggcaccctg ctgcatgagg
     4381 acctgaacct cgtgcagcgc gtgctgcgcg acctcgcgaa cgaccagacc cagaccatac
     4441 gcatcgactc gcgcatgcag tacgagaccc tgcgcggctt cggcgaggaa tacacgccca
     4501 ccgcggtctc caggctggag cactacaagg gcgagcgccc gatcttcgac ctctacaaca
     4561 tcgaccagga gatcgagcgc gcgctggccc ggcgcgtgga cctgaagtcg ggcggctatc
     4621 tcatcatcga ccagaccgag gcgctgacga cggtggacgt caacaccggc ggctatgtcg
     4681 gcgcgcgcaa tttcgacgac accatcttca agaccaacct cgaggcggcc ctggccatcg
     4741 cgcggcagct gcgcctgcgc aacctgggcg gcatcatcat catcgacttc atcgacatgg
     4801 tgcgcgagga gcaccaggag gccgtgctgg ccgagctgcg caagcagctg gcgcgcgacc
     4861 gcaccaagat cacggtcagc ggcttcaccc agctcggcct ggtcgagatg acgcgcaagc
     4921 gcacccgcga atcgctggcc cacatgctgt gcgagccttg cccgacctgc gaaggccgcg
     4981 gccaggtcaa gacggcgcgc agcgtctgct atgacatcct gcgcgagatc ctgcgcgagg
     5041 cgcggcagtt caaccccaag gagttccgcg tcgtcgcctc ggccggcgtg gtggaaatgc
     5101 tgctggacga ggaaagccag catttggccg gcctgtccga cttcatcggc aagccgatct
     5161 cgctgcagac cgagccttcg atgtcgcccg agcagtacga catcgtcctc ctgtagcgcc
     5221 ccgccatgca gttctccatc atcatcccga gctggaacaa ccttgcgcat ctgcagctgg
     5281 tgctgcgcag catccgcgag cattcggcat acgcgcatca ggtcatcgtg catgtcaacg
     5341 acggcagcga cggcagccgc gaatgggtgc gctcgcaagg cctggacgcg acgtacagcg
     5401 acgccaacat cggcatctgc tacgccgtca acgaggcggc gatgctcgcg aggcacgact
     5461 acatcgtcta catgaacgat gacatgtatt gctgccccgg ctgggacagc gcgctggcgc
     5521 gacgcattgc gaccatgccg agcgacctct tcatgctgtc gggcaccatg gtcgagccgc
     5581 gcgacagcgg caacccctgc gtcgcggtgg gcccctacgg cgacgcggtc gacaacttcc
     5641 aggagcaatc gctgctggcc gcgctgccgc gcctcaagcg cgccgattgg tatggcgcga
     5701 cctggccgcc gacgctggtg caccgacgct ggtggttccg ctgcggtggc tacagcaccg
     5761 agctcagccc cggcatgagc agcgacaacg atttctcgat gaagctgtgg ttcgcgggct
     5821 gtcggcattt catcggcgtc ggcgattcgc tggtctatca cttccagcag aagtccaccg
     5881 gcaaggtcgt caagaacgac gggcggcgcc agttcctcgc caaatggggc atgacgcagt
     5941 cgaccttcga tcgccactac ctgcggcgcg gcgagccgct gcagggagat gccttgccgg
     6001 agccccagga cacgccggcc ctgcgctggg ccttacgcaa ggcggcgctc aagcgccgct
     6061 tcgcgtgatc aatctcgcct ggcgaagcgc cgtttgagcc agaacgaagg ggcgagcagc
     6121 cccgggcagg cggcgtagac cagcaggcgg cagcgcgcgc ccagcggcac gtccgcagca
     6181 gcccagaagc agcgcagcct gccgcgcagc gtggcggcgt ggaacaagtc gtggatgtag
     6241 cgctcgcgca ccagctgcgg cgcaatcacg ggctgtacgt cggccagcgc gcccatcagc
     6301 tcggcgtcgc gcagcagcag gggcagttcc accagtgcgt ggcggttgtt cttcttcagc
     6361 cgcatgatca cgtcttgcgc cgacaggctg cgcacgcgtc gcgagatgcc gccgcggcgg
     6421 tattgcacca gcggttcggc cagcgtgatg gcaccgccgc tgccgatggc gcgaaacacc
     6481 atgatcaggt cttcggccac cacaccctcc ggcagcgggc cgaagcattc cagcagccgc
     6541 cgggtccagg cctgggccgc gcccaccaca tggggcagct tgttcgccca atcgcgcaga
     6601 ttcctgtagg cgccgaggtc cgagggcgtg atgatcgctt gcacgcggcc ttcggcatcg
     6661 atgtcctgca gcggggccgc gatcaggtcg agcttcctgc cgcttgcgag ccaggcctcg
     6721 acgacacggc tgcagcgctg cggccgcgag acgtcgtcgc ctgccgcgac gaac
//
LOCUS       DASUCY010000332         3371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_987219, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000332 DASUCY010000000
VERSION     DASUCY010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987219"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="VFV25_06520"
     CDS             complement(<1..960)
                     /locus_tag="VFV25_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEY1393010.1"
                     /translation="MFQAQLTVDRDFEVASLDRRLFGVFVEHLGRCVYGGIYEPGHPT
                     ADAQGFRADVAALTRELGATIVRYPGGNFMSGYDWEDGVGPQRERPRRLDLAWFSTET
                     NQVGTHEFLAWCHHVGVEPMFGVNLGTRGPDEARRYLEYCNHPGGTALSELRRAHGAE
                     APLGIKFWCLGNEMDGPWQICRKTPDEYGRIALETAKLMRMVDPSVELAACGSSAYDM
                     PTYGVWEDRVLEHCFEQVDYLSLHSYFVKPPTDASTEGFLAQIETNARYIEDTLAIAD
                     AVAARKRSAKRLMLSFDEWNVWYRARHGEHLKQTGWPEAPRLLE"
     gene            complement(961..2292)
                     /locus_tag="VFV25_06525"
     CDS             complement(961..2292)
                     /locus_tag="VFV25_06525"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY1393011.1"
                     /translation="MLKRTLLLLVVLLAGCGRPADTSGKTEITLMRFFGSCDAKYAQV
                     ADASQGVGECGIITTLVNEFNATNKDGIVVRTQIGEWGPYYDQLTARLVAKDVPTIAV
                     MHESALGDYVRRKLVLPLDADFERLGIPKDDFTDHARRGVTFNGQIYALPFDTWAWLW
                     HINTRLMKQAGLLGEDGQPKLPHSPEELLAQARQFRQRTGKPYFAWAVANETAANDRT
                     FLTLVAQQGAQLFAPDGKTIRMQQPAVAKALALMQQLYAEGHVKPNLDYAGANQAFLN
                     GEAGIVFVGTWTIDQFLRESQKPDSPLQGSYTVRPFPQLYEHPAVFADGHSWALFKGG
                     AADAKAHEASLKFLKFIWERSAEWARTGHLPVSKSVAQSPAFRALPLREKLTEITTTG
                     IAMPGNVPTQRAIELLIGEDVANLLISAKPVAEVQASVEKRVNTALKKARR"
     gene            complement(2292..3128)
                     /locus_tag="VFV25_06530"
     CDS             complement(2292..3128)
                     /locus_tag="VFV25_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEY1393012.1"
                     /translation="MNSLLRRGWFDRFVMAALCLLALAWLAPLLWVLALSFKPNEFLL
                     THTDVFLAPPYTLANYADILQASQVFRWLGNSAVVALAQTAGVLTLSSLAGYGFARCE
                     FPGRRALFAVVMFGLAVPAQSTFIPLHRLFSDWNLQNTLAGLALPGLAAPFGVYLMTQ
                     YFKALPAELEEAAMLDNTSRLKTFTHIILPLSLPAQATLGIFTFLACWNDYLWPLVIA
                     TKPEMYTLTRGLGSMQTNFAQSEGLGFLMAQAVFAGLPALTLYLFFQRHLVTAVAGTG
                     RS"
     gene            complement(3125..>3371)
                     /locus_tag="VFV25_06535"
     CDS             complement(3125..>3371)
                     /locus_tag="VFV25_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEY1393013.1"
                     /translation="VLVALIEVIREFQVFPQIMLMTNGGPNNTTRSIVQFIYEQAFMQ
                     LSLGYATAASQVLLAIMLLGVSAQLWLERDKGQGGRK"
BASE COUNT          543 a         1080 c         1208 g          540 t
ORIGIN      
        1 ttccaggagg cgcggcgcct cgggccagcc ggtctgcttc aggtgctcgc cgtggcgggc
       61 gcggtaccag acgttccact cgtcgaagga cagcatcagc cgcttggctg agcgcttgcg
      121 cgcggcgacg gcgtcggcga tcgcgagggt gtcctcgatg tagcgcgcgt tcgtctcgat
      181 ctgcgcgagg aagccctcgg tgctcgcatc cgtcggcggt ttcacgaaat agctgtgcag
      241 cgacaggtag tccacctgct cgaagcagtg ctccagcacg cggtcttccc agacgccgta
      301 ggtcggcatg tcgtaggccg aggagccgca ggcggccagc tcgacgctcg ggtccaccat
      361 gcgcatcagc ttcgctgtct ccagcgcgat gcggccgtat tcgtccggcg tcttgcggca
      421 gatctgccag gggccgtcca tctcattgcc caggcaccag aacttgatgc ccagaggcgc
      481 ctcggcgccg tgcgcgcggc gcagctcgga cagcgccgtg ccgcccggat ggttgcagta
      541 ttcgaggtag cggcgcgcct cgtccggccc gcgcgtgccg aggttgacgc cgaacatcgg
      601 ctcgacgccg acgtgatggc accacgcgag aaattcgtgc gtgccgacct ggttggtctc
      661 ggtcgagaac caggccaggt ccagccgccg cgggcgctcg cgctgcgggc cgacgccgtc
      721 ctcccagtcg tagcccgaca tgaagttgcc gccggggtag cgcacgatgg tggcgccgag
      781 ctcgcgcgtc agcgcggcca cgtcggcgcg gaagccctgg gcgtcggccg tcgggtggcc
      841 gggctcgtag atgccgccgt agacgcagcg acccaggtgt tcgacgaaga cgccgaagag
      901 gcgccgatcc agcgaggcga cctcgaagtc gcggtcgaca gtgagttgtg cctggaacat
      961 ctatctgcga gccttcttca gggcggtgtt gacgcgcttc tcaacgctgg cctgcacctc
     1021 ggcgaccggt ttggccgaga tcagcagatt ggccacgtcc tcgccgatca gcagctcgat
     1081 ggcgcgttgc gtgggcacgt tgccgggcat cgcaatgccg gtggtcgtaa tctcggtgag
     1141 cttctcgcgc agcggcagcg cgcggaaggc agggctttgc gccacgctct tgctgaccgg
     1201 caggtggccg gtgcgcgccc actcggcgct gcgttcccag atgaacttca ggaacttcag
     1261 gctggcctcg tgggccttgg cgtccgcggc gccgcccttg aacagcgccc acgagtgtcc
     1321 atcggcgaag acggccgggt gctcgtagag ctgcgggaat ggccgcaccg tgtagctgcc
     1381 ctgcagcggc gagtcgggct tttgggattc gcgcaggaac tggtcgatgg tccaggtgcc
     1441 gacgaagacg atgccggcct cgccattcag gaaagcctgg ttggcgcccg cgtagtcgag
     1501 gttgggcttc acgtggcctt cggcatagag ctgctgcatc agcgccagcg ccttggccac
     1561 ggccggctgc tgcatgcgga tcgtcttgcc gtccggcgcg aagagctggg cgccctgctg
     1621 ggcaaccagg gtcaggaaag tgcggtcgtt ggcggccgtt tcgttggcca cggcccaggc
     1681 gaaatagggc ttgccggtgc gctgcctgaa ctgccgcgcc tgggccagca gctcctcagg
     1741 cgaatgcggc agcttcggct ggccgtcctc gcccagcagg ccggcctgct tcatcagccg
     1801 ggtgttgatg tgccacagcc aggcccaggt gtcgaagggc agggcgtaga tctggccgtt
     1861 gaacgtgacg ccgcggcgag cgtggtcggt gaaatcgtcc ttggggatgc ccaggcgctc
     1921 gaagtcggcg tccagcggca gcacgagttt gcggcgcaca tagtcgccca gggccgactc
     1981 gtgcatgacg gcgatggtcg gcacgtcctt ggcgacgagg cgggccgtga gctggtcgta
     2041 gtagggaccc cattcgccga tctgcgtgcg cacgacgatg ccgtccttgt tcgtcgcgtt
     2101 gaactcgttg accagcgtcg tgatgatccc gcactcgccg acgccctggg acgcgtcggc
     2161 cacctgggcg tacttggcat cgcagctgcc gaagaagcgc atcagcgtga tctcggtctt
     2221 gcccgaggta tcggccgggc ggccgcagcc ggccagcagc acgacgagaa ggagcagggt
     2281 gcgcttcaac atcaggaccg ccccgtgccc gccacggcgg tcaccaaatg gcgctggaag
     2341 aagagataga gcgtcagcgc tggcagcccg gcgaagacgg cctgggccat caggaagccc
     2401 aggccctcgg actgcgcgaa attcgtctgc atggatccca ggccgcgggt cagcgtatac
     2461 atctcgggct tggtggcgat gaccagcggc cagaggtagt cgttccagca ggccaggaaa
     2521 gtgaagatgc cgagcgttgc ctgcgccggc aggctcagcg gcaggatgat gtgcgtgaag
     2581 gtcttcagcc gcgaggtgtt gtccagcatc gcggcctcct ccagctcggc cgggagggcc
     2641 ttgaagtact gcgtcatcag gtagacgccg aagggcgcgg ccagccccgg cagcgcgagg
     2701 ccggccagcg tgttctgcag gttccagtcc gagaacagcc ggtgcagcgg gatgaaggtg
     2761 gactgtgccg gcaccgcgag gccgaacatg acgacagcga acagcgcgcg ccggcccggg
     2821 aactcgcagc gcgcgaagcc gtagccggcc agcgaggaca gcgtcagcac gccggccgtc
     2881 tgcgccagcg ccacgacggc gctgttgccc agccagcgga agacctgcga ggcctgcagg
     2941 atgtcggcgt agttggccag cgtgtagggc ggcgccagga agacgtcggt gtgcgtcagc
     3001 aggaattcgt tgggcttgaa cgaaagcgcc agcacccaca gcagcggcgc cagccaggcc
     3061 agcgcgagca ggcacagcgc cgccatgaca aagcggtcga accaaccgcg gcgcagcagc
     3121 gagttcattt ccgacctccc tggcccttgt cgcgctccag ccagagctgg gccgatacgc
     3181 ccagcagcat gatggccagc agcacctggg aggcggcggt cgcgtagccg agcgagagct
     3241 gcatgaaggc ctgctcgtag atgaactgca cgatggagcg cgtcgtgttg ttcggcccgc
     3301 cgttggtcat cagcatgatc tgcgggaaga cctggaattc gcggatgacc tcgatcaggg
     3361 cgacgaggac g
//
LOCUS       DASUCY010000333         2056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_681788, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000333 DASUCY010000000
VERSION     DASUCY010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681788"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VFV25_06540"
     CDS             <1..529
                     /locus_tag="VFV25_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004532569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEY1393014.1"
                     /translation="APARRELLIERGTLKRPLGGAVSQARARALGYELGGVATTRACS
                     WNRAPIDRMSNLNVEPGDADFEQIIASVAYGVVMHTNCSWSIDDSRNKFQFGCEYGRV
                     IRQGRLAEVVRNPNYRGISATFWRSLAMVGDASTFQVMGTPYCGKGEPSQVIRVGHAA
                     PVCKFSKVAVFGGAA"
     gene            529..1878
                     /locus_tag="VFV25_06545"
     CDS             529..1878
                     /locus_tag="VFV25_06545"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEY1393015.1"
                     /translation="MMMSLMNLTQRSYFEALADAVCQAKADERLSLYLKAEATDFIRF
                     NHAAVRQATHVLQAHATLALVRGSRSAESTLTLSGDLAHDTAALRAEHVRLAHDLPQL
                     PEDPYLLLPDAVVNTTREDHGVIPRPDEVIAAVAEHAKGLDLVGLYAGGPVVRAYADS
                     RGQRNWHRVESFQFDWCIYHAADKAVKTSYAGARWQTEEFVRRVHEAGERVKLLAREA
                     RTLEPGAYRAYFAPAAMGELLGTLSWGGFGLKERRTGTSSLTRLANGEAQLHRAFQLG
                     EDLGASSAPAFTPKGFTRPESVALITDGRAADSLNSPRSAREYGLPTNGASAQEAPEA
                     LRLAAGSLAESDVLRELGTGLYISNLHYLNYSDRQACRMTGMTRFACFWVEDGQLVAP
                     LNVMRFDDGFLRLFSDEGLVALTDAQEWLPESNTYGSRMLSSITTPGALVSGFQLTL"
BASE COUNT          336 a          724 c          674 g          322 t
ORIGIN      
        1 tgcgccggcc aggcgcgagc tgctcatcga gcgaggcacc ctgaagcggc cactgggcgg
       61 cgccgtctct caggcgcgtg cccgcgccct gggctatgag ctgggcggcg tcgcgacgac
      121 gcgcgcctgc tcctggaacc gtgcgcccat cgaccgcatg agcaatctca atgtcgagcc
      181 gggcgatgcc gacttcgagc agatcatcgc ctcggtcgcc tacggcgtcg tgatgcacac
      241 caactgctcg tggagcatcg acgactcgcg caacaagttc cagttcggct gcgaatacgg
      301 ccgcgtgatc cgccaggggc gcctcgccga ggtcgtgcgc aaccccaatt accgcggcat
      361 ctcggccacc ttctggcgct cgctcgcgat ggtgggcgac gcgtcgacct tccaggtcat
      421 gggcacgccc tactgcggca agggcgagcc ttcgcaggtg atccgcgtcg gccacgcggc
      481 accggtttgc aagttctcca aggtggcggt gttcggagga gccgcatgat gatgatgagc
      541 ctcatgaacc tcactcagcg cagctatttc gaggccctgg ccgatgcggt gtgccaggcc
      601 aaggccgacg agcgcctgag cctctacctc aaggccgagg cgaccgactt catccgcttc
      661 aaccacgccg ccgtgcgcca ggccacgcac gtgctgcagg cccatgcgac gctggccctg
      721 gtgcgcggct cgcgcagcgc cgaatcgacg ctgacgctca gcggcgatct ggcgcacgac
      781 actgcggccc tgcgcgccga gcacgtcagg ctggcgcacg acctgccgca gctgcccgag
      841 gatccttatc tgctgctgcc cgacgccgtg gtcaacacca cgcgcgaaga ccacggcgtg
      901 atcccgcggc ccgacgaagt catcgcggcc gtggccgagc atgcgaaagg cctggacctg
      961 gtcggccttt acgccggcgg gccggtggtg cgcgcctacg ctgacagccg cggccagcgc
     1021 aactggcacc gcgtggaaag cttccagttc gactggtgca tctaccacgc cgccgataag
     1081 gccgtgaaga cgagctatgc cggtgcacgc tggcagaccg aggaattcgt gcgccgcgta
     1141 cacgaggcgg gcgaacgcgt gaaactgctc gcgcgcgagg cgaggacgct cgagccgggc
     1201 gcctaccgcg cctatttcgc gccggccgcg atgggcgagc tgctgggcac cctgtcctgg
     1261 ggcggcttcg gcctcaagga aagaaggacc ggcacctcgt cgctgacccg cctggccaac
     1321 ggcgaggcgc agttgcaccg cgcattccag ctcggcgaag acctgggcgc gagcagcgcc
     1381 ccggctttca cgcccaaggg cttcactcgc ccggaaagcg tggcactgat taccgatggc
     1441 cgcgccgccg actcgctcaa ttcgccgcgc tcggcgcgcg aatacggcct gccgaccaat
     1501 ggtgcctcgg cccaggaagc gcccgaggcc ctgcggctgg cggctggcag cctggcggaa
     1561 agcgacgtgc tgcgcgagct gggcacgggg ctttacatca gcaacctgca ctacctgaac
     1621 tacagcgacc gccaggcctg ccgcatgacc ggcatgaccc gcttcgcctg cttctgggtc
     1681 gaggacggcc agctcgtcgc gccgctgaac gtgatgcgct tcgacgacgg cttcctgcgc
     1741 ctcttcagcg acgagggcct ggtcgcgctc accgacgccc aggaatggct gccggagagc
     1801 aacacctacg gctcgcgcat gctcagctca atcacgacgc ccggtgccct ggtctcgggc
     1861 ttccagctga cgctgtaagc ccgtcagcga tcgatcgggc atcggcggct cgcggacggt
     1921 ggcatcgccc tgtcaccgcc gcgggcttta cggttgcgaa tcattcttat ttataattgc
     1981 cacatcgcaa cgcgtattca gctccgaaga tgatcgtctg tgtctgccag cgggtttcca
     2041 gcaaggacat cgaacg
//
LOCUS       DASUCY010000334         2046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1212316, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000334 DASUCY010000000
VERSION     DASUCY010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1212316"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="VFV25_06550"
     CDS             <1..768
                     /locus_tag="VFV25_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014897799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEY1393016.1"
                     /translation="YNKKIEEAAARGEKLPNPFSLTPESPEPLERLPHIVIMIDELAD
                     LMMVVGKKIEELIARLAQKARASGIHLILATQRPSVDVITGLIKANIPTRIAFQVSSK
                     IDSRTILDQMGAEALLGQGDMLYLAPGTGLPVRVHGAFVSDDEVHRVVEYLKSQGEPN
                     YIEGILEGGNLDDEGSNLDGSPAGGGEGESDPMYDQAVAVVLQHRRASISLVQRHLRI
                     GYNRAARLLEQMEKSGLVSGMATNGNRDILVPARTAE"
     gene            768..1376
                     /gene="lolA"
                     /locus_tag="VFV25_06555"
     CDS             768..1376
                     /gene="lolA"
                     /locus_tag="VFV25_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HEY1393017.1"
                     /translation="MKRLLIALALLAAGTARADSIATLKAFVSGVKTGRASFTQVVTS
                     PDGAKKKTSSGSFEFSRPNRFRFDYAKPYEQLIVGDGQKVWLYDADLNQVTVRSFSKA
                     LGSTPAALLAGSGLEKDFDLKALPDEGGLQWVQASPKAKDSGFQSLKVGFGKGGQLAA
                     LEILDGFGQRSRLDFAQVEANAAEPAERFHFTPPAGADVLAQ"
     gene            1480..>2046
                     /locus_tag="VFV25_06560"
     CDS             1480..>2046
                     /locus_tag="VFV25_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY1393018.1"
                     /translation="MKTSDPGLDAPLAERLRPRTLDEVIGQQHLLGPGKPLRAAFESG
                     QPHSMILWGPPGVGKTTLARLMADAFDALFIPISAVLGGVKDIRDAVEQAQQARHRGG
                     LLGGAQRTIVFVDEVHRFNKAQQDAFLPHVESGLFTFIGATTENPSFEVNSALLSRAT
                     VHVLQPLSDDDLRVMLERGRALLGAPPLS"
BASE COUNT          366 a          700 c          660 g          320 t
ORIGIN      
        1 tacaacaaga agatcgagga agccgccgcg cgcggcgaga agctgcccaa ccccttcagc
       61 ctcacgccgg agtcgcccga gccgctggag cgcctgcctc acatcgtgat catgatcgac
      121 gagctcgccg acctgatgat ggtggtgggc aagaagatcg aggaactcat cgcgcgcctg
      181 gctcaaaagg cacgcgcctc cggcatccac ctcatcctcg ccacgcagcg gcccagcgtg
      241 gatgtgatca cgggcctcat caaggccaac atcccgacgc gcatcgcctt tcaggtgagc
      301 agcaagatcg acagccgcac catcctcgac cagatgggtg ccgaggcgct gctggggcaa
      361 ggcgacatgc tctacctcgc gcccggcacc ggcctgccgg tgcgggtgca cggcgccttc
      421 gtcagcgacg acgaggtgca ccgcgtcgtc gagtatctca agagccaagg cgagccgaat
      481 tacatcgagg gcattctcga aggcggcaac ctcgacgacg agggcagcaa tctcgacggc
      541 agccccgcgg gtggcggcga aggcgagtcc gaccctatgt acgaccaggc cgtggccgtg
      601 gtgctgcagc accggcgcgc gtcgatctcg ctggttcaaa gacatttgcg catcggctac
      661 aaccgggcag cacgcctgct ggaacaaatg gagaagtcgg ggctcgtatc gggcatggcc
      721 accaacggca atcgcgacat cctcgtgccc gcccgcactg cagaatgatg aaacgactcc
      781 ttatcgctct tgccctgttg gccgccggca cggcccgggc cgattcgatc gccacgctga
      841 aggccttcgt cagcggcgtc aagaccgggc gtgccagctt cacccaggtc gtgacctcgc
      901 ccgacggggc gaagaagaag acctccagcg gaagcttcga gttctcccgg cccaatcgct
      961 tccgcttcga ctacgccaag ccttacgagc agctgatcgt cggcgacggg cagaaggtct
     1021 ggctctacga tgcggacctc aaccaggtca ctgtgcgcag cttcagcaaa gccctgggtt
     1081 ccacgccggc cgcgctgctg gccgggtcgg gtctggagaa ggatttcgat ctcaaggccc
     1141 tgcccgacga aggcgggctg cagtgggtgc aggccagccc caaggccaag gacagcggct
     1201 tccaatcgct taaagtcggt ttcggcaagg gcggccagct cgcggccctg gagatcctcg
     1261 acggcttcgg ccagcgctcg cggctggatt tcgcgcaagt cgaggccaat gcagccgagc
     1321 cggcagagcg cttccacttc acaccgccgg ccggcgccga cgtgctggcg cagtagcggc
     1381 cccacctctg cggcccccga cagcgccctt cggcgaaggg cgtcgttcct gcccctgagg
     1441 gttgagtaaa gtggcaaagc cacattacgc aagagtcaca tgaagacttc ggatcccggt
     1501 ctcgatgctc cgttggccga gcggctgcgg ccgcgcacgc tcgacgaggt gatcggccag
     1561 cagcacctgc tggggccggg caagccgctg cgtgcggcct tcgaatcggg gcagccgcat
     1621 tcgatgatcc tctgggggcc gcccggcgtg ggcaagacca cgctggcccg cttgatggcc
     1681 gacgccttcg acgcgctctt catcccgatc tcggcggtgc tgggcggcgt gaaggacata
     1741 cgcgacgccg tggaacaggc ccagcaggcg cgccaccgcg gaggattgct cggcggtgca
     1801 cagcgcacca tcgtcttcgt cgatgaggtg caccgcttca ataaggcgca gcaggacgcc
     1861 ttcttgcccc acgtcgagtc gggcctcttc accttcatcg gcgccactac cgagaacccg
     1921 tccttcgagg tgaattcggc actgctgtcg cgcgccacgg tgcatgtgct gcagccactg
     1981 tccgacgacg acttgcgcgt gatgctggag cgcggccgag ccctgcttgg cgcgccgccg
     2041 ctcagc
//
LOCUS       DASUCY010000335         3222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_521495, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000335 DASUCY010000000
VERSION     DASUCY010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521495"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="VFV25_06565"
     CDS             complement(<1..427)
                     /locus_tag="VFV25_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY1393019.1"
                     /translation="MKKSLLALAVLSAFAGAASAQSSVTLYGRVDVALGKAPFDTAKQ
                     EANGSGSRVGLRGVEDLGGGLAAVFDIQHRFNAQNGAQTSTRFWHGLSVVGLKGDFGQ
                     VLLGRNYTTSYTYGQVAADPWGGETVVSGNYMKTTATPAA"
     gene            703..1335
                     /gene="coq7"
                     /locus_tag="VFV25_06570"
     CDS             703..1335
                     /gene="coq7"
                     /locus_tag="VFV25_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HEY1393020.1"
                     /translation="MPSSSPIDALIISADNALRTVFGRHQAARACPQAAQDDNLDQAQ
                     RRLSGALMRVNHVGEICAQALYTAQALSTRDAALQAHFEQAARDETDHLAWTEQRLAE
                     LGDRASWLNPLWYAGAFGIGLLAGRMGERVSLGFVVETERQVEQHLESHLDRLPEADA
                     ASRAIVSQMRVDEARHALQAENAGAVRLPLPVRGLMRAAAKVMTTTAHYV"
     gene            complement(1356..1778)
                     /locus_tag="VFV25_06575"
     CDS             complement(1356..1778)
                     /locus_tag="VFV25_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY1393021.1"
                     /translation="MECTINWQPASGMAFIAETGSGHLLTMDGAPDGGGRNLAPRPME
                     TVLAGTGGCTAYDVVLILKRGRHEVRGCQVKLNAERATADPKVFTKIHMHFTVTGRGL
                     PQAAVERAVQMSHEKYCSASIMLAKTAVITTSVEIIED"
     gene            2111..>3222
                     /gene="ilvA"
                     /locus_tag="VFV25_06580"
     CDS             2111..>3222
                     /gene="ilvA"
                     /locus_tag="VFV25_06580"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEY1393022.1"
                     /translation="MARAPRRSSSTPSLPRATGRPDLTGYLQKILTARVYDVAIESPL
                     ESARSLSRRLHNKILLKREDNQPVFSFKLRGAYNKMVHLAPEQLQRGVICASAGNHAQ
                     GVALSAARLGCRAVVVMPVTTPHVKVDAVKALGGEVVLQGDSYSDAYLHAVELEKSQG
                     LSFVHPFDDPDVIAGQGTIAMEILRQHQGPIDAIFVAIGGGGLISGVAAYIKSVRPEI
                     KVIGVQTTDSDAMVRSVKAGKRVQLGDVGLFADGTAVKLVGEETFRVARALVDDYVVV
                     DTDAVCAAIKDVFQDTRSILEPSGAMSVAAIKQYVETHKCKGQTFVAITCGANMNFDR
                     LRFVAERAEVGEEREALFAVTIPEERGSFKRFCELIG"
BASE COUNT          563 a         1060 c         1033 g          566 t
ORIGIN      
        1 gggcagcagg cgtcgccgtc gtcttcatat agttgcccga cacgacggtt tcaccacccc
       61 agggatccgc ggccacttgg ccataggtgt acgaggtggt gtagttgcga cccagcagca
      121 cttggccgaa gtcgcccttc aggccgacca cggacagacc gtgccagaag cgggtcgagg
      181 tctgcgcgcc gttctgggcg ttgaagcggt gctggatatc gaacacggca gccagaccgc
      241 caccgaggtc ttccacgcca cgcaggccga cacggctgcc ggagccgttg gcttcctgct
      301 tggcggtgtc aaagggcgcc ttgcccaggg cgacgtcaac acgaccatag agggtgaccg
      361 acgattgggc cgaggcagca cctgcgaaag cgctcaggac tgcgagagcg agcagagatt
      421 ttttcattgc taactatctc caaggttgaa acaagaggtc ccaaaaaagc ccctgcgcgc
      481 acggcgtgcc tgcgcgaagg ggccgatcag gccaacctcc gtatcggaag ctgccgccat
      541 tcgaccagac gtgccggggg ctgaccaggg ttttggagga gaaaaaggcc tttccgttgt
      601 cgcataacaa cgtactctgg atgagccgca ccatcatcgt tacaacttgt cgggcaacaa
      661 tcacattcat agtcctatcg gcaaaattca tcgagctgac acatgccctc ctcttctcct
      721 atcgacgccc tgatcatttc tgccgacaac gctttgcgca ccgtttttgg caggcatcag
      781 gcagccaggg cctgcccgca ggccgcgcag gacgacaatc tcgatcaggc ccagcgccgc
      841 ctgtccggcg ccctgatgcg ggtcaaccat gtgggggaga tctgcgcgca ggcgttgtac
      901 acggcccagg cgctcagcac tcgagatgcc gccttgcagg cccatttcga gcaggcggct
      961 cgcgatgaaa ccgatcatct ggcctggacc gaacagcgcc tcgccgagct cggcgaccgc
     1021 gccagctggc tcaatccgct ttggtatgcc ggcgccttcg gcatcggcct gctggctggc
     1081 cgcatgggcg agcgcgtgag cctgggcttt gtcgtggaaa cggagcgcca ggtcgagcag
     1141 catctggaga gccacctgga ccggctgccc gaggccgatg cggcctcgcg cgccatcgtg
     1201 agccagatgc gcgtggacga ggcgcggcat gcactgcagg ccgaaaacgc cggcgccgtt
     1261 cgcctgccgc tgccggtgcg cggcctgatg cgcgccgcag ccaaggtgat gacgacgacc
     1321 gcgcactatg tctgagtgcg caggcgctcc aggcctcagt cctcgatgat ttccacgctg
     1381 gtcgtgatca cggccgtctt ggccagcatg atgctggcgg agcaatattt ctcgtgcgac
     1441 atctgcaccg cgcgctcgac cgctgcctgg ggcaggcccc gtccggtcac ggtgaaatgc
     1501 atgtggatct tggtgaacac cttgggatcg gcggtggcgc gctcggcgtt cagcttgacc
     1561 tggcagccgc gcacctcatg gcggccgcgc ttgagtatca gcaccacgtc gtaggcggtg
     1621 cagccgccgg tgccggccag cacggtttcc attggccgcg gtgccagatt gcggccaccg
     1681 ccgtccggtg cgccatccat cgtcagcaga tgcccactac ccgtctcggc gatgaaagcc
     1741 atgcccgaag ccggctgcca gttgatggtg cattccatgg tgattccgat ctcgcacaga
     1801 ttttccggcg tctgtcgact ttacccgccg atctggggac ccatccctaa gagacagcgc
     1861 tacacggacc agaaaactgg cctatactgg cgacattgca gtgcagcacg agcctttctg
     1921 gcagcggcac tccagtttgt ctcctccacc ctcctcgttt ggtggattcg gcccgaaacc
     1981 gcaaggtttc gggccctttt tctttctgct ctcggctcac cggccgggca gccgaggtgc
     2041 ttggctgcag cgcagcaaaa cggccgcgta tcatgcctgc cccgtcacac ccatcgatcc
     2101 ggagtcagcc atggcccgcg cgccccgccg cagttcgtcc acgcccagct tgccacgcgc
     2161 gaccggccgg cccgatctga cgggttatct gcagaagatc ctgacggcgc gggtctatga
     2221 cgtggcgatc gagtcgccgc tggagtccgc gcgcagcctc tcgcgccgcc tgcacaacaa
     2281 gatactgctt aagcgcgagg acaaccagcc cgtcttcagc tttaagctgc gcggcgccta
     2341 caacaagatg gtgcacctgg cgcccgaaca gctgcagcgc ggcgtgatct gcgcctcggc
     2401 cggcaaccac gcgcagggcg tggccttgag cgcggccagg ctgggctgcc gcgcggtggt
     2461 cgtgatgccg gtgaccacgc cccacgtcaa ggtcgatgcc gtcaaggccc tgggcggcga
     2521 ggtcgtgctg caaggcgaca gctattcaga tgcctatctg cacgcggtcg agctggagaa
     2581 gagccagggc ctgagcttcg tccacccctt cgatgacccc gatgtcatcg ccggccaggg
     2641 cacgatcgca atggagatcc tgcgccagca ccaggggccg atcgatgcga tcttcgtcgc
     2701 catcggtggc ggcgggctga tctcgggcgt ggcggcctat atcaaatcgg tgcgtcccga
     2761 gatcaaggtg atcggcgtgc agaccaccga ctcggacgcg atggtgcgct cggtgaaggc
     2821 gggcaaacgc gtgcagctgg gcgacgtggg cctgttcgcc gatggcaccg ctgtcaagct
     2881 ggtcggtgaa gagaccttcc gtgtcgcgcg cgcgctggtc gacgactacg tggtggtcga
     2941 caccgacgcg gtctgcgcgg ctatcaagga cgtgttccag gacacgcgct ccatcctcga
     3001 gccgtccggc gcgatgagcg tggcggcgat caagcaatac gtcgagacgc acaagtgcaa
     3061 gggccagacc ttcgtggcca tcacctgcgg cgccaacatg aacttcgacc gcctgcgctt
     3121 cgtggccgag cgcgccgaag taggcgagga gcgcgaggcg ctgtttgccg tcaccatccc
     3181 cgaggagcgc ggctcgttca agcgcttctg cgagctgatc gg
//
LOCUS       DASUCY010000336         5096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1260208, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000336 DASUCY010000000
VERSION     DASUCY010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 5096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5096
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1260208"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1124)
                     /locus_tag="VFV25_06585"
     CDS             complement(<1..1124)
                     /locus_tag="VFV25_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEY1393023.1"
                     /translation="MSSAAHFSLLVVDDEPDLRTLYELTLLREGYDVETASTVEDALL
                     HLKDRTYSALITDMRLPDGSGLDLLRRLEETGRREKTIVITAYGSAENAVEALKAGAY
                     DYLTKPVDLKQFRTVVASALGRVESPAPTVQPGSLPSAGAQQAASKAVRATLAASQQP
                     AGRAPAAASGAGNGALARMAGSSRAMQEVRSLIEKVARSMAPAMVRGESGTGKELVAR
                     AIHEVSSRSAQPFVAVNCSAIPEQLLEAEFFGYRKGAFTGANEDREGVFQAARGGTLF
                     LDEIGDLPLSMQSKLLRAIQERAVRPVGAVAEVPLDVRIVSATHKDLTAEVQAGRFRQ
                     DLYYRLNVIQILVPPLRERLDDPPAICDRVLARIAADAGV"
     gene            complement(1144..3045)
                     /locus_tag="VFV25_06590"
     CDS             complement(1144..3045)
                     /locus_tag="VFV25_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY1393024.1"
                     /translation="MSSNAQRAAARAAASRPAMEESWFGALGVSEDSQRDDAARALDA
                     ESRYDGDWKAAQGRVAQDSRFISRQARRLVGQARPSFQRIYRAFLGARAALGLALLVS
                     QLVSGLFSAKATWGALVGVCAAYAVASCGVWLLSRRAHGAFATSEVNYLRRREWLATI
                     GVDILAFSALNVLARGASLNYVALLVLPVMMAGLLTRRLYALAVTAGVTLILLFSAWL
                     AVLAGGEPIQSITQAGLAGMGFFVISLLAGELAGGLAREELTARGSLELARQQAQLNR
                     LVIEEMQDGVLVVDRRGHVRAANPAARLLLADSGMTRPAPFQLRRVEAWAPMVQAVEE
                     AFATGTWPSGGRELSLSFRGGSTRHLRLRVRFTRRRDPKAPEEFCVLFIEDVRTAEAR
                     LRQEKLAAMGRVSAGIAHEIRNPLAAIAQANALLGEDATTPAQRQLTQMVADNVERLK
                     RIVDDVMEVAPGSAPMPAVLDLGPLVASICSEWARTAGLPLGERSPLKVDLPEREAAA
                     AKFDADHLRRVLVNLLDNAYRHGSKTAAAVWVRLYTEEGGQRLRLSVASDGEPIAPEV
                     ERYLFEPFFSTRSRGTGLGLYICRELCERYGASIDYRQRSAPERHSNDFFLSLKREAL
                     DQTEARLHL"
     gene            complement(3042..3293)
                     /locus_tag="VFV25_06595"
     CDS             complement(3042..3293)
                     /locus_tag="VFV25_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HEY1393025.1"
                     /translation="MVMLRYLLILLFVLLLLWFARGGGSSRPRSGGAAPRRGSPQPMV
                     ECAHCGVHLPRDEALAGPGGHYCCEEHRRIGAASPGGGS"
     gene            complement(3320..4159)
                     /gene="ccsA"
                     /locus_tag="VFV25_06600"
     CDS             complement(3320..4159)
                     /gene="ccsA"
                     /locus_tag="VFV25_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEY1393026.1"
                     /translation="MILSPSLTPYGQLALGLSSLFALLGYAFAAAMGEGSGRRAALLI
                     GWLAHALAIAVDTMGLGATQPGVRFGFAPVLSVTLWLVLAVYVVESRLVPLPGVRRVL
                     ALLGMTAVALALFFPGEFHLHAVSAWAPLHWILGIASYGLFGAAVLHAALLSHAERSL
                     RLKPNGGHGVPAQGPLGLPLLRLEKLTFRFVAAGFAVLSATLLIGWWFANPWRWDHKN
                     VFSILAWIVFAGLLAGRRGFGWRGQRALRWLYVGAVLLLLGYVGSRFVLEVVLKRTVE
                     TGL"
     gene            4321..>5096
                     /locus_tag="VFV25_06605"
     CDS             4321..>5096
                     /locus_tag="VFV25_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HEY1393027.1"
                     /translation="MASALSERLSRLVKTMRGQARITEANVQDMLREVRMALLEADVA
                     LPVVRDFVARVKDKALGQEVIGSLSPGQALVGIVHKELAATMGEGIVDIDLATQPPAV
                     ILMAGLQGAGKTTTTAKLAKHLIEKRKKKVLTVSADVYRPAAIEQLKTVSKQAGAEWF
                     PSQANDSPREIALGALDYAKKHFFDVLLVDTAGRLAIDEALMAEIRELHAALHPIETL
                     FVVDAMQGQDAVNTAKAFKEALPLTGIVLTKLDGDSRGGAA"
BASE COUNT          863 a         1823 c         1741 g          669 t
ORIGIN      
        1 ctcacgccgg catcggccgc gatgcgggcc agcacgcggt cgcagatcgc gggcgggtcg
       61 tccaggcgct cgcgcagcgg cggcaccagg atctggatca cgttgagccg gtagtacaag
      121 tcctggcgga aacgcccggc ctgcacctcg gccgtcaggt ccttgtgcgt cgcgctgacg
      181 atgcgcacgt ccagcggtac ctctgccacc gcgcccaccg gacgcacggc gcgctcctgt
      241 attgcgcgca gcagcttgga ctgcatcgac agcggcaagt cgccgatctc gtcgagaaac
      301 agcgtgccgc cgcgcgcggc ctggaagacg ccttcgcggt cctcgttcgc gccggtgaag
      361 gcgcccttgc ggtagccgaa gaactcggcc tccagcagct gctcggggat ggcgctgcaa
      421 ttgacggcga cgaagggctg ggcgctgcgc gaactcacct cgtggatcgc gcgggccacc
      481 agctccttgc cggtgcccga ttcgccgcgc accatcgccg gcgccatgct gcgcgccacc
      541 ttctcgatca gcgagcgcac ctcctgcatg gcccgtgagg agcctgccat gcgcgccagc
      601 gcgccgttgc ctgcaccact tgccgccgcg ggcgcgcgcc ctgccggctg ctggctcgcg
      661 gcaagcgtgg cgcggacggc cttggacgcc gcctgctgcg caccagccga cggcaggctg
      721 cccggctgca ccgtcggggc gggcgattcg acgcgcccga gcgccgaagc caccacggtg
      781 cggaactgct tgaggtccac cggcttggtg aggtagtcgt aggcaccggc cttgagcgcc
      841 tccacggcgt tctcagccga gccataggcc gtgatgacga tggtcttctc gcgccgtccg
      901 gtctcttcca gccggcgcag caggtcgagc ccgctgccat cgggcagccg catgtcggtg
      961 atgagcgcgc tgtaggtgcg atccttcaga tgcagcagcg catcctccac cgtgctcgcc
     1021 gtttcgacgt catagccttc gcgcagcagc gtgagctcgt agagcgtgcg caggtcgggc
     1081 tcatcgtcga ccacgaggag gctgaaatgc gcagccgagc tcatgcatct ctccgggcaa
     1141 ggttcatagg tggagtcggg cctcggtctg atcgagcgct tcgcgcttca ggctcaggaa
     1201 gaaatcattg ctgtggcgtt ccggcgcgga gcgctgacgg tagtcgatgc tggcaccgta
     1261 gcgctcgcac aactcccgac aaatatacaa gcccagcccg gtgccgcggc tgcgcgtgga
     1321 gaagaagggc tcgaacagat agcgctccac ctcgggcgca atgggctcgc cgtcgctggc
     1381 cacgctcagg cgcaggcgct ggccgccttc ctcggtgtag agccgcaccc acaccgcggc
     1441 ggccgtcttg ctgccatggc gataggcgtt gtccagcagg ttgacgagca cgcggcgcag
     1501 atgatcggcg tcgaatttcg ccgcggccgc ctcgcgctcc ggcagatcca ccttcaaggg
     1561 gctgcgctcg cccagcggca ggcccgcggt gcgcgcccac tcgctgcaga tcgaggcgac
     1621 cagcggtccg aggtccagca ctgccggcat cggcgcgctg ccgggcgcca cctccatcac
     1681 gtcgtcgacg atgcgcttca ggcgctcgac attgtcggcc accatctgcg tgagctggcg
     1741 ttgcgccggc gtcgtcgcgt cttcaccgag cagcgcattg gcctgggcga tggcggccag
     1801 cggattgcga atctcgtgcg cgatgccggc cgagacgcgc cccatcgcgg cgagcttttc
     1861 ctggcgcagg cgcgcctcgg cggtgcgcac gtcctcgatg aagagcacgc agaattcctc
     1921 aggggccttg ggatcgcgcc ggcgcgtgaa gcgcaccctt aggcgcaggt gccgggtgct
     1981 gccgccgcga aagcttaagc tcaactcgcg tccgccggac ggccaggtgc cggtggcgaa
     2041 ggcctcctcg accgcctgca ccatcggcgc ccaggcctcc acacggcgca gctggaaggg
     2101 cgcgggccgc gtcatgcccg aatcggccag cagcaggcgc gcggccgggt tggcggcgcg
     2161 tacatggccg cggcggtcga ccaccagcac gccgtcctgc atctcctcga tcaccaggcg
     2221 attgagctgg gcctgctggc gcgccagctc caggctgccg cgcgcggtga gctcttcgcg
     2281 cgccaggccg ccggccagtt cgccggccag cagcgagatg acgaagaagc ccatcccggc
     2341 caggccggcc tgggtgatgg actggatggg ctcgccgccg gccaacacgg ccagccaggc
     2401 gctgaagagc aggatcagcg tcacgccggc cgtgacggcc agggcataca gccgtcgcgt
     2461 gagcaggccc gccatcatca ccggcagcac cagcagcgcg acgtagttga ggctcgcgcc
     2521 gcgcgccagc acgttcaagg ccgagaaggc caggatgtcg acgcctatcg tggccagcca
     2581 ttcgcggcgg cgcaggtagt tgacctccga cgtggcgaat gcaccgtgcg ccctgcgcga
     2641 cagcagccac acgccgcagg acgcgaccgc ataggcggcg cacacgccca ccaatgcgcc
     2701 ccacgtcgcc ttggcggaaa acaggcccga cacgagctgg ctgaccagca atgccagacc
     2761 cagcgcggcg cgcgcgccga ggaaggcccg atagatgcgc tggaacgagg gtcgcgcctg
     2821 gccgaccaat cggcgcgcct ggcgcgagat gaagcgcgag tcctgcgcca ccctgccctg
     2881 cgcggccttc cagtcgccgt cgtagcgcga ttcggcgtcc agcgcgcgtg ccgcatcatc
     2941 gcgctgcgaa tcctcgctca cgcccagggc gccaaaccag gattcctcca tcgccggcct
     3001 ggacgccgcc gcgcgcgccg cggcgcgctg tgcgttacta ctcatgaccc gccgcccggc
     3061 gatgccgcgc caatgcggcg atgctcctcg cagcagtaat ggccgccggg cccggccagg
     3121 gcctcgtcgc gcggcaggtg cacgccgcag tgcgcgcatt cgaccatggg ctgcggcgaa
     3181 ccgcggcgcg gcgcggcgcc gccgctacgc gggcggctgc tgccgccgcc gcgagcgaac
     3241 cacagcagaa gcagcacgaa gagcaggatc agcagatacc tcaacatcac catgcgctct
     3301 caagaaaccg aagcggcgct cataggcccg tttccacggt gcgcttgagc acaacttcga
     3361 gcacaaagcg cgagcccaca tagccgagca gcagcagcac cgcgcccaca taaagccagc
     3421 gcagcgcacg ctgcccgcgc cagccgaagc cccgccggcc ggccagcagg cccgcgaaca
     3481 cgatccaggc caggatggag aacacgttct tatggtccca gcgccaggga ttggcgaacc
     3541 accagccgat cagcagcgtg gccgacagca ccgcgaagcc ggcggccacg aagcggaagg
     3601 tcagcttctc cagtctcagc aggggcaggc cgagcgggcc ttgcgccggc acgccatggc
     3661 cgccattggg cttgagccgc agcgagcgct cggcatgact cagcagcgcc gcatgcagca
     3721 cggcagcgcc gaagaggccg taggaagcga tgcccaggat ccagtgcagc ggcgcccagg
     3781 ccgagacggc atgcagatgg aactcgccgg ggaagaacag cgccagcgcc accgccgtca
     3841 tgcccagcag ggccagcacg cgccgcacac ccggcagcgg cacgagccgg ctttccacca
     3901 catagactgc aagcaccagc caaagcgtga ccgacagcac cggcgcaaag ccgaagcgca
     3961 cgccgggctg cgtggcgccc aggcccatgg tgtccaccgc gatggccagc gcatgggcca
     4021 gccagccgat caggagggcg gcgcgccgtc cggagccttc gcccatcgcc gccgcgaagg
     4081 cgtagccgag caaggcgaag agactggaga gccccagggc cagctggccg tagggggtga
     4141 ggctgggcga taaaatcatc gtccacagtg tactttcggg cgagcgcgca gcgcgcgagc
     4201 ccgggtgcgc cggggtcgcc gcatgaaggc ggcacgcggt gcgcgagatg ccgcaccgca
     4261 tcggcaacga acagcgaaag aagagcattc cgcgccgcgg gatgcgaaag gctccccatc
     4321 atggcctccg ccctctcaga acgcctgtcg cgcctggtca agaccatgcg cggccaggcc
     4381 cgcatcaccg aagccaacgt gcaggacatg ctgcgcgaag tgcgcatggc cctgctcgag
     4441 gccgatgtgg ccctgcccgt cgtgcgcgac ttcgtggccc gcgtgaagga caaggcgctg
     4501 ggccaggaag tgatcggctc gctgtcgccg ggccaggccc tggtgggcat cgtgcacaag
     4561 gaactggccg ccacgatggg cgaagggatc gttgacatcg acctcgcgac ccaaccccct
     4621 gccgtgatcc tgatggccgg cctgcaaggc gccggcaaga ccacgaccac cgccaagctg
     4681 gccaagcacc tgatcgagaa gcgcaagaag aaggtgttga ccgtctcggc cgacgtctac
     4741 cggcccgcgg ccatcgagca gctcaagacc gtgagcaagc aggccggcgc cgagtggttc
     4801 ccctcgcagg cgaacgacag cccgcgcgag atcgcgctgg gcgcgctgga ctatgcgaag
     4861 aagcatttct tcgatgtgct gctggtcgac accgccggcc ggctcgcgat cgacgaagcg
     4921 ctgatggccg agatccgcga gctgcatgcg gcgctgcatc cgatcgagac gctcttcgtc
     4981 gtcgacgcga tgcagggcca ggacgccgtc aacacggcca aggccttcaa ggaagcattg
     5041 ccgctgaccg gcatcgtgct gaccaagctc gacggcgatt cgcgcggcgg cgcggc
//
LOCUS       DASUCY010000337         4013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1436926, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000337 DASUCY010000000
VERSION     DASUCY010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1436926"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="VFV25_06610"
     CDS             complement(<1..832)
                     /locus_tag="VFV25_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HEY1393028.1"
                     /translation="MLHSTHLMRKALACLALAALVAPLSARDGSGVFFDDFSYADTDA
                     LLRGGWALRSKPGHPGVPGASWEPTGATLIDDPERRSNRLLRLTARTDGTPAGTAQVQ
                     LCHQRKYLEGTYAARLRFSDAPVQGAGGDPVIETFYAVGPLRFDFDPEFSEIDWEYLP
                     NGGWGSERTRLYGITWQTVRIEPWLAYNQADEKFGPLGGWHVLLMQVAGGKTRHYLDG
                     RLLAEHGGRNYPVVPMSINFSLWFSPGGLRAPSSQPRVYQEDVDWVFHARGQVLSPAQ
                     V"
     gene            complement(840..2183)
                     /locus_tag="VFV25_06615"
     CDS             complement(840..2183)
                     /locus_tag="VFV25_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1393029.1"
                     /translation="METSAQAASPPEDSRPRRSPISWVPTLYFAEGLPFYTVALIAGL
                     MYKSLGVPNDQIARWTGLLGLAWVFKPLWSPFLEAASSKKLLVVVFQATGAVALGLVA
                     LALQLPGWFAASIAVLFVVAFASATHDIAADGLYIASLSAKQQASYAGWQGAFFNAAK
                     FISLGGLVILAGHLEKRMAVTHAWALIFLALASMLAALATYHSWALPALRRRVEPSGE
                     DRTTRVLHTLSDVIADFFRKPGIWLAILFIILFRAGEGQIQTIGPLFLREARAAGGLG
                     LSTEDVGLVYGTAGTVAFLIGSVLGGYFTAWLGLKRALFFLILAMNLPNLVFYWLSVA
                     TPTDLRLISLGLSLEMFGYGFGFVGVILFIMQVVAVGKYQTAHYALGTGVMQLGFVLF
                     KTISGDVQTAMGYEHFFLWVLVSALPVLVLSRFLPIRGPARAAESAQPAGPAAQA"
     gene            complement(2226..3725)
                     /gene="nagZ"
                     /locus_tag="VFV25_06620"
     CDS             complement(2226..3725)
                     /gene="nagZ"
                     /locus_tag="VFV25_06620"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEY1393030.1"
                     /translation="MSQPEINLHAGRLVMVDIQGRSLDAATADFLRSHRIRGVCLFRS
                     NLGSEAEVRQLVADLRDAMGPEALIGIDQEGGSVVRATFLPQPPAAMALGAADDAALC
                     EAVGAAVSRGLRSVGINWNFAPVLDVNNNPANPVIAERSFGEDPTRVARLAGAWMRGA
                     LREGVACCVKHFPGHGDTHVDSHHALPTVDKSIAELEALELKPFRALASEAPAMMTAH
                     IVYPQIDAEHPATLSRRILGGLLREAMGYDGVVITDALMMKAIHERYGHARAAVLTLQ
                     AGADLALAQGSREEQFAAVQAIAEALRSGELAPQRAAQASARLDALARRYPIRPAPYE
                     AAQRTADDALMHEAWARGLVAIGSPQPPHRLQPLRVYTQRNVPSDGVSEAGPSAEQVA
                     ALFSAFPDTELVALDSLDSFDWAQVPVDGRLNVLASNHRARYGERARSWRPDLHLVLW
                     NPYPTLDIAAPALVSWGYADGALAAVRGWLEGRLQVQAASPVALRIGAG"
     gene            complement(3722..>4013)
                     /locus_tag="VFV25_06625"
     CDS             complement(3722..>4013)
                     /locus_tag="VFV25_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005664296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEY1393031.1"
                     /translation="EISVLPEHVLLDPSKVEVSLYAHLALIGKAPVRALQHIRALNAE
                     AELADQLGVPCGRAVLFITRVGYLASGMPVELTHSYCRSDHYGFVAEMRRDA"
BASE COUNT          662 a         1338 c         1434 g          579 t
ORIGIN      
        1 cgacctgggc cggcgacagc acctggccgc gggcgtggaa gacccagtcc acatcctcct
       61 gatagacgcg cggctgtgaa ctgggcgcac gcagcccacc cggcgagaac cagaggctga
      121 agttgatgct catcggcacc accggatagt tgcgcccgcc gtgctcggcc agcagccggc
      181 cgtccaggta atgccgcgtc ttgccgccgg ccacctgcat cagcagcaca tgccagccgc
      241 cgagtgggcc gaacttctcg tcagcttggt tataggccag ccagggctcg atgcgcaccg
      301 tctgccaggt gatgccgtag agccgggtgc gctcgctgcc ccagccgccg ttgggcaggt
      361 attcccaatc gatctcgctg aactctgggt cgaagtcgaa gcgcagcggg ccgacggcat
      421 agaaggtctc gatcaccggg tcgccgccgg cgccttgcac cggcgcgtcg ctgaagcgca
      481 ggcgtgcggc ataggtgcct tccaggtatt tgcgctgatg gcaaagctgc acctgggccg
      541 taccggctgg cgtgccatcg gtgcgcgcag tgaggcgcag caagcggttg ctgcgccgct
      601 cgggatcgtc gatcagcgtg gcgccggtcg gctcccagct tgcgccgggc acgccggggt
      661 ggcctggctt gctgcgcagc gcccagccgc cgcgcagcag ggcgtccgtg tcggcgtagc
      721 tgaagtcgtc gaagaagacg ccgctgccgt cacgtgccga aagcggagcg accaaagcgg
      781 ccagcgccag acaggccagc gccttgcgca tcagatgggt gctgtgcagc acagcgaggt
      841 caggcctggg ccgcaggccc ggcaggctga gcgctctccg ccgcgcgagc cgggccgcgt
      901 atcggcaaga agcgcgacag caccagcacc ggcagggccg ataccagcac ccagaggaag
      961 aagtgctcgt aacccatcgc ggtctggacg tcgccgctga tggtcttgaa cagcacgaag
     1021 cccagctgca tcacgccggt gcccagcgcg taatgggcgg tctggtattt gccgaccgcc
     1081 accacctgca tgatgaagag gatcacgccg acgaagccga agccatagcc gaacatctcc
     1141 aggctgagcc cgaggctgat gagcctgaga tcggtgggcg tcgcgacgct gagccaatag
     1201 aacacgaggt tgggcaggtt catcgcgagg atgaggaaga acagcgcgcg cttcagcccc
     1261 agccaggcgg tgaaatagcc gccgagcacg ctgccgatca ggaaggcgac ggtgcccgcc
     1321 gtgccataga ccaggcccac gtcctcggtg ctcaggccca gcccgccggc cgcgcgcgcc
     1381 tcgcgcagga agagcgggcc gatggtctgg atctggccct cgccggctcg gaacaggatg
     1441 atgaagagga tggccagcca gatgcccggc ttgcggaaga agtccgcgat cacgtcgctc
     1501 agggtgtgca gcacgcgggt ggttctgtcc tctccgctgg gctccacgcg gcggcgcagg
     1561 gcgggcaggg cccagctgtg atacgtcgcc agcgctgcca gcatgctggc cagcgccagg
     1621 aagatcaggg cccaggcatg ggtcacggcc atgcgctttt cgaggtggcc ggccaggatc
     1681 accagtccgc ccagcgagat gaacttggcc gcgttgaaga aggcgccttg ccagccggcg
     1741 tatgaggcct gctgcttggc cgagaggctg gcgatataaa ggccgtcggc cgcgatgtca
     1801 tgcgtggccg aggcgaaggc cacgacgaag agcacggcga tgctcgccgc aaaccagccg
     1861 ggcagctgca gcgccagcgc caccagcccc aatgcgaccg cgcccgtggc ctggaagacc
     1921 acgaccagca gcttcttgct gctggccgcc tcgaggaagg ggctccacag cggcttgaac
     1981 acccaggcca ggccgagcag cccggtccag cgcgcgatct ggtcgttggg cacgcccagg
     2041 ctcttgtaca tgaggccggc gatcagcgcg accgtgtaga agggcaggcc ctcggcgaag
     2101 tagagcgtgg gcacccagct gattggcgaa cggcgcggcc ggctgtcttc tggaggtgat
     2161 gcggcttggg ctgaagtctc catatgggct ctttctcttt ggcctttgaa tcgaaggcgg
     2221 atcgttcagc cggctccgat gcgcagggcg accggggagg cggcctgaac ctgcagccgg
     2281 ccttcgagcc agccgcgcac ggcggcgagc gccccatctg catagcccca gctcaccagg
     2341 gccggggccg cgatgtccag ggtcgggtag ggattccaca gcaccagatg caggtccgga
     2401 cgccaggagc gtgcgcgctc gccatagcgc gcacggtggt tggaggcgag cacgttcagg
     2461 cggccgtcca cgggcacctg cgcccagtcg aagctgtcga gggaatcgag ggccaccagc
     2521 tcggtgtcgg ggaaggcgct gaacagcgcg gccacctgct cggcgctcgg cccggcctcg
     2581 gacacgccgt cgctgggcac gttgcgctgg gtatagacgc gcaatggctg caggcggtgc
     2641 ggcggctggg gcgagccgat ggcgaccagg ccgcgcgccc aggcctcgtg catcagcgcg
     2701 tcgtcggccg tgcgctgtgc ggcctcatag ggcgcgggcc ggatcgggta gcgccgcgcc
     2761 agcgcgtcca ggcgcgcgct cgcctgcgcg gcgcgctgcg gcgccagctc gccgctgcgc
     2821 aaggcctcgg cgatggcctg cacggcggcg aactgctcct cgcggctgcc ctgggccagc
     2881 gccaggtcgg ccccggcctg cagcgtcagc accgcggccc gcgcatggcc gtagcgctcg
     2941 tgtatggcct tcatcatcag cgcgtcggtg atgacgacgc cgtcgtagcc catcgcctcg
     3001 cgcaacaggc caccgaggat gcgcctggac agcgtggccg gatgctcggc gtcgatttgc
     3061 ggatagacga tgtgcgccgt catcatcgcc ggcgcctcgc tggcaagcgc gcggaaaggt
     3121 ttgagctcca acgcctccaa ttcggcgatc gacttgtcga ccgtaggcag cgcatggtgg
     3181 gagtcgacat gggtatcgcc gtggccgggg aaatgcttga cgcagcaggc cacgccctcg
     3241 cgcagcgcgc cgcgcatcca ggcgccggcc agtctggcga cgcgggtcgg atcctcgccg
     3301 aagctgcgct cggcgatcac cgggttggcc gggttgttgt tgacgtccag caccggcgcg
     3361 aaattccagt tgatgcccac gctgcgcagc ccgcgcgaga cggcggcgcc taccgcttcg
     3421 cacagcgccg cgtcatcggc cgcgcccagc gccatcgcag ccggaggctg tggcaggaag
     3481 gtcgcgcgca ccaccgagcc gccttcctgg tcaatgccga tcagggcctc ggggcccatg
     3541 gcgtcccgca ggtcggcgac gagctgtcgc acctcggctt cgctgccgag gttgctgcgg
     3601 aacaggcaaa cgccgcggat ccggtggctg cgcaggaaat ccgcagtggc ggcatcgagg
     3661 gaccgaccct ggatgtcgac catcacgagg cggccggcgt gcaggttgat ttcaggctgg
     3721 ctcatgcgtc tcgtctcatc tcggccacga agccgtagtg atcgctgcgg caataggaat
     3781 gggtcagctc gaccggcatg ccggaagcca aatagccgac gcgagtgatg aagagtaccg
     3841 cgcggccgca aggtacgccc agctggtcgg caagctccgc ctcggcgttg agggcgcgta
     3901 tgtgctggag cgcgcgcacc ggcgccttgc cgatcagggc cagatgggcg tagagcgaga
     3961 cctcgacctt ggaagggtcg agcagcacat gctcgggcag cacgctgatt tcg
//
LOCUS       DASUCY010000338         4225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1388942, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000338 DASUCY010000000
VERSION     DASUCY010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1388942"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VFV25_06630"
     CDS             complement(<1..529)
                     /locus_tag="VFV25_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY1393032.1"
                     /translation="MKVLLIDDHPLILSALQTVIKGLGDDVTVVGADSAKAARATLEA
                     DADFDLVLLDLQLGDANGFDLLADLRSSYPALPVVVVSASDRASDVIRSIDMGAMGFV
                     PKRSSNEQLFEALRLVMSGGIYVPPITMGSEPLPPAYAETAPSYLRVVKQEAGQSDFQ
                     KTPSLASLGLTPRQTD"
     gene            complement(696..1148)
                     /locus_tag="VFV25_06635"
     CDS             complement(696..1148)
                     /locus_tag="VFV25_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEY1393033.1"
                     /translation="MKTFEHLRELEALVGQEIGVSAWTTVDQARIDRFAEATGDHQWI
                     HTDPQRAAAGPFGTTIAHGFLTLSLLSAFLGSAFAVADARMGVNYGLNRVRFMAPVPV
                     GSRLRARLTLVGFDAIEGGAQLAIEATLEREGSAKPVCVAQSLTRFYL"
     gene            complement(1269..1883)
                     /locus_tag="VFV25_06640"
     CDS             complement(1269..1883)
                     /locus_tag="VFV25_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEY1393034.1"
                     /translation="MPVIVVANPKGGVGKSTLATNLAGWFARQGQAVMLGDADRQQSA
                     RTWLSLRPAELPRIATWDTDHGDIVKPPKGTTHVVLDTPAGLHGKRLDEVMKLADHVL
                     VPLQPSIFDIYATRDFVAQLLERRKHEKVKIGVVGNRVKEGTIAADQLHSFLDGLSVP
                     VLTYLRDTQNYVHLAARGLTLWDVAPGRVEKDLLQWEPVTAWAS"
     gene            1964..2599
                     /locus_tag="VFV25_06645"
     CDS             1964..2599
                     /locus_tag="VFV25_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY1393035.1"
                     /translation="MARILVLAAHPDLRHSRVNQALLSAARALAGDKLVVRDLYAIYP
                     DYVIDAGAEQALAEQADLIVWQHPIHWYHMPPLMKLWVDEVLDYGWAYGKGGTALRGK
                     DLWLVASTGGPEAAYHPSGYNRYFFDAFLPPYEQTAMLCGLRFLPPLVLHGAHAVGRA
                     ELDAHAANFSQRLASYPDWPELADMEACPECEVPPIDRPHEEKAAAAALAS"
     gene            2609..>4225
                     /gene="kefC"
                     /locus_tag="VFV25_06650"
     CDS             2609..>4225
                     /gene="kefC"
                     /locus_tag="VFV25_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HEY1393036.1"
                     /translation="MEHAAWLTSSLVFLAAACFAVPLSKALGLGSIIGYLAAGILIGP
                     FGLRLVDDPETILHFAEFGVVLMLFLVGLELEPRRLWALRRPIFGWGSLQVLGCTAVL
                     IALALAFRVPWRWAVAGALGLSLSSTAIALQVMSERNLLPTDTGQAGFSILLFQDVAA
                     IPILALLPLLGGSAEASGGNAWWLAAKAVGVVGAIVLGGRLLLRHALRWIARSDSTEI
                     FTAASLGLVVGIASLMQAVGLSAALGAFLGGVLLAESEYKRELETDVEPFKGLLLGLF
                     FMAVGMSVDFAALLAHPGVVLALLLGFMLLKAAVLWGLARAIALPYPERPVFTLLLAQ
                     GGEFAFVVFQQASSEGVFSAEVGSVLIGAVALSMLVTPLLLVAMDRWLLPRYANCAAP
                     AVSELDEPQDAPVLICGFGRYGQIIGRLLYANGMKATLLDHDPEQIEVLRRFGFRIYY
                     GDATRLDLLRTAGAATARVLVVAIDDMEQSLAVVDIARQHFPQLAIVARARNVQHHYA
                     LRDRGVEMIERETFESALRSGRSALELMGLH"
BASE COUNT          587 a         1432 c         1474 g          732 t
ORIGIN      
        1 catcggtctg gcgcggcgtg aggcccagcg aggcgagcga aggcgtcttc tggaagtcgc
       61 tctgcccggc ctcctgcttc acgacgcgca gatagctcgg tgcggtttcg gcataggccg
      121 gcggcagggg ttcgctgccc atggtgatcg gcggcacata gatgccaccg gacatcacca
      181 gccgcagcgc ctcgaagagc tgctcgttgc tggagcgctt gggcacgaag cccatggcgc
      241 ccatatcgat cgagcggatg acgtcgctgg cgcggtccga ggccgacacg acaactaccg
      301 gcagcgccgg gtagctggag cgcaggtcgg cgagcaggtc gaagccgttg gcatcgccca
      361 gctgcaggtc cagcagcacg aggtcgaaat cggcatcggc ctcgagcgtg gcgcgggcgg
      421 ccttggcgct gtcggcgccg accacggtga cgtcgtcgcc caggcccttg atgacggtct
      481 gcagggccga gaggatcagc ggatggtcgt cgatcagcag cactttcatg ggctcggtcc
      541 tcgggcgctg aagcgccggt catgtcgtcg gcaaatgcag ccgatcgtaa cggagcttgc
      601 gccgcaacgc ccccctccta aggggggatc gagccatgag ccatccaatg tcagctgact
      661 tgcccctcac ccttcttagc cgctgagagc cgccgctaca agtagaaccg cgtcagcgat
      721 tgcgcgacgc agaccggctt ggccgagcct tcgcgctcca acgtggcctc gatcgcgagc
      781 tgggcgccgc cctcgatggc atcaaagccg accagcgtca ggcgcgcccg caggcggctg
      841 ccgaccggta ccggggccat gaagcgcacg cggttcaggc catagttgac gcccatgcgc
      901 gcgtcggcca ccgcgaaggc gctgcccagg aaggccgaca acaggctgag cgtgaggaag
      961 ccgtgcgcga tcgtcgtgcc gaagggccca gcagcggcgc gctgcggatc ggtgtggatc
     1021 cattgatggt cgccggtggc ctcggcgaag cggtcgatgc gcgcctggtc caccgtggtc
     1081 caggcgctga cgccgatctc ctggccgacc agggcctcga gttcgcgcag gtgttcgaag
     1141 gtcttcatga tgccgggcgc ttttttgcgc ccaggagttg cgacgcgatc atgctaggca
     1201 gcccgggctg cgcgcgcagt cgcctttgcg gcatccctcg gacatggaat cggcctgccg
     1261 ggcggcgctc aggacgccca tgccgtcacc ggctcccact gcagcaggtc cttctcgacg
     1321 cgaccgggcg ccacgtccca cagcgtgagg ccgcgggcgg ccagatgcac gtagttctgc
     1381 gtgtcgcgca gatacgtcag tacgggcacg ctcaggccgt cgaggaagct gtgcagctgg
     1441 tcggcagcaa tggtgccttc cttgacgcgg ttgccgacca cgccgatctt gaccttctcg
     1501 tgcttgcggc gctccagcag ctgggccacg aagtcgcgcg tggcatagat gtcgaagatg
     1561 ctgggctgca gcggcaccag cacatggtcg gccagtttca tcacctcgtc caggcgcttg
     1621 ccgtgcaatc cggcgggggt gtcgagcacc acatgggtcg tgcccttggg cggcttgacg
     1681 atgtcgccgt ggtcggtatc ccaggtggcg atgcgcggca gctcggccgg gcgcagcgag
     1741 agccaggtgc gggcggactg ctgccggtcc gcgtcgccca acatcaccgc ttgtccctgg
     1801 cgggcgaacc agccggccag attggtcgcc agtgtggatt tgcccacgcc tcccttcggg
     1861 ttcgccacca cgatcaccgg catctgcgca cctccgtgcc gcaattcgcg gcgatcgggt
     1921 atgttagcgg cgactttctc gatgacctta caggaacacc cccatggccc gcatcctcgt
     1981 gcttgccgcc caccccgacc tgcgccattc gcgcgtcaac caggccttgc tgagcgccgc
     2041 gcgtgcgctg gcaggcgaca agctggtggt acgtgacctc tatgcgatct accccgacta
     2101 tgtgatcgac gcgggcgccg aacaggccct ggcggagcag gccgatctga tcgtctggca
     2161 gcaccccatc cactggtacc acatgccgcc gctgatgaag ctatgggtgg acgaggtgct
     2221 ggactacggc tgggcctacg gcaagggcgg cacggcgctg cgcggcaagg atctctggct
     2281 ggtcgcctcg acgggcggcc ctgaggcggc ctatcacccc agcggctaca accgctattt
     2341 cttcgacgcc ttcctgccgc cctacgagca gacggccatg ctctgcggcc tgcgtttcct
     2401 gccgccgctg gtgctgcacg gcgcgcatgc ggtgggccgg gccgagctgg acgcgcacgc
     2461 ggcgaacttc agccagcggc tggccagcta cccggactgg cccgagctgg ccgacatgga
     2521 ggcctgcccc gaatgcgaag tcccgccgat cgaccggccg cacgaagaaa aagccgcagc
     2581 cgcggcgctt gcgagctgag ggggccgcat ggaacacgcc gcctggctga ccagcagcct
     2641 cgttttcctg gccgccgcct gtttcgccgt gcccttgtcc aaggcgctgg ggctgggctc
     2701 catcatcggc tacctcgccg ccggcatcct gatcggcccc ttcgggctca ggctggtcga
     2761 cgacccggag accatcctgc atttcgccga gttcggcgtc gtgctgatgc tcttcctggt
     2821 cggcctggag ctggagccgc gccggctgtg ggcgctgcgc cggcccatct tcggctgggg
     2881 cagcctgcag gtgctgggct gcaccgcggt gctgatcgcg ctggcgctgg ctttccgcgt
     2941 gccctggcgc tgggcggtgg cgggcgccct gggcctgtcc ttatcgtcca ccgcaatcgc
     3001 gctgcaggtg atgagcgagc gcaacctgct gcccaccgat acgggccagg cgggcttttc
     3061 catcctgctg ttccaggacg tcgcggcgat tcccatcctc gcgctgctgc cgctcctggg
     3121 cggcagcgcc gaggccagcg gcggcaatgc ctggtggctc gccgccaagg cggtgggcgt
     3181 ggtcggcgcc atcgtgctgg gcgggcgatt gctgctgcgc catgcgctgc gctggatcgc
     3241 gcgcagcgac tcgaccgaga tcttcaccgc cgcctcgctg ggtctggtgg tcggcatcgc
     3301 ctcgctgatg caggccgtgg gcttgtcggc agcgctgggc gccttcctgg gcggcgtgct
     3361 gctggccgag agcgaataca agcgcgagct ggagactgac gtcgagccct tcaagggcct
     3421 gctgctgggt ctgttcttca tggccgtggg catgtctgtc gactttgccg ccttgctcgc
     3481 ccaccccggc gtcgtgctgg cgctgctgct gggcttcatg ctcctgaagg ccgcggtgct
     3541 ctggggcctg gcgcgggcca ttgccctgcc ttacccggag cgtccggttt tcacgctgct
     3601 gctggcccag ggcggcgaat tcgccttcgt cgtcttccag caggccagca gcgagggcgt
     3661 gttcagcgcc gaggtcggtt cggtgctgat cggcgcggtc gcgctgtcga tgctggtcac
     3721 gccgctgctg ctcgtggcga tggaccgctg gctgctgccg cgctatgcca actgcgcagc
     3781 gcctgcagtg agcgagctgg acgagccgca ggacgcgccg gtgttgatct gcggcttcgg
     3841 ccgctatggg cagatcatcg gccgcctgct ctatgccaac ggcatgaagg ccacgttgct
     3901 ggaccatgac cccgagcaga tcgaggtgct gaggcgcttc ggctttcgca tctactacgg
     3961 cgacgcgacc cggctcgacc tgctgcgcac cgccggcgct gcaacggcgc gcgtgctggt
     4021 cgtggcgatc gacgacatgg agcagagcct ggcggtggtc gacatcgcgc gccagcattt
     4081 ccctcagctg gccatcgtgg cgcgggcgcg caatgtgcag caccactatg cgctgcgcga
     4141 ccgcggcgtg gagatgatcg agcgcgagac cttcgagtcg gcgctgcgca gcgggcgcag
     4201 cgcgctcgag ctgatggggc tgcat
//
LOCUS       DASUCY010000339         4389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1292447, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000339 DASUCY010000000
VERSION     DASUCY010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4389
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1292447"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1314)
                     /locus_tag="VFV25_06655"
     CDS             complement(<1..1314)
                     /locus_tag="VFV25_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY1393037.1"
                     /translation="MTRSSDIDRGTPLRTSSAEVITLEIDGCPITVPAGTSVMRAAAQ
                     AGVMVPKLCATESLEPFGSCRLCLVEIEGHAGYPASCTTPVATGMKVRTQSPRLQALR
                     RGVMELYLSDHPSDCVSGAGHVDCELHETARQLGVRISRFGHEGASHVAEAKDESNPY
                     FTYDPAQCIVCSRCVRACEEIQGSYALTVDGRGFASRIVAGAREPFMASECVSCGACV
                     QACPTDALLEKTVIECGAPERSVVTTCAYCGVGCGLVAELKDDRVVRMTPWKGGQANE
                     GHACVKGRFAWGYATHPDRVTRPMIRRSTSDPWTEVSWDEAIGHAAAEFRRIQAAYGK
                     EAVGGITSSRCTNEETYLVQELVRAAFGNNNVDTCARVCHAPTGYALGQAFGTSAGTQ
                     SFRSVDQVDVFMVIGANPTEAHPVLASRIKRRLRQGAKLIVLDPRR"
     gene            complement(1311..2861)
                     /locus_tag="VFV25_06660"
     CDS             complement(1311..2861)
                     /locus_tag="VFV25_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEY1393038.1"
                     /translation="MSTVVRVFVPRDSAARAVGADAVAQALVAQARQPIELVRSSSRG
                     LFWLEPLVEVDTPAGRVGYGPVAEHDVAPLLAAGMLRGAAAPAPSLGLVEALPYLQRQ
                     RRITFAHAGLTDPLSLADYEAHGGWAGLHKALTLAPEVVVQQVGESGLRGRGGAGFPA
                     GIKWRTVAQARADRKTIVCNADEGDSGTFADRMLLEGDPYLLIEGLLIAALATGARQA
                     FVYIRSEYPDAVATFGEAVRRAREAGMLGPAVLGSQRQIDIQVRMGAGSYVCGEETAL
                     LESLEGKRGMVRSKPPVPAVAGLFGQPTVVNNVLTLAAVPAILEKGPEFYRSLGRGRS
                     TGTQAFQLAGNIRHGGLVELGFGESLRELLYEWGGGTASGRPIKAVQLGGPLGSYLPP
                     SQWDVPLTYEDTAAIGASLGHGGIVVHDDRADMAKLARHAMAFCAAESCGKCTPCRIG
                     STRGAEIIDRIRAGRERMQHVVLLRELCDTMTHASLCAMGGMTPNPVLSALDHFPQDF
                     GLPGEEKP"
     gene            complement(2858..3316)
                     /locus_tag="VFV25_06665"
     CDS             complement(2858..3316)
                     /locus_tag="VFV25_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HEY1393039.1"
                     /translation="MTTVRAIVQELSHLEGALLPILHRVQEALGFVPREALPVIAQAL
                     NLSRAEVQGVLGYYSHFRQQPAGRCVVQVCAAESCQAMGGEALLMHARATLGCSVAQP
                     ASGDGAYTVTPVYCLGLCALSPAVTIDGQAHARMTPARLDTLLAAKELLP"
     gene            3991..>4389
                     /locus_tag="VFV25_06670"
     CDS             3991..>4389
                     /locus_tag="VFV25_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011880151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HEY1393040.1"
                     /translation="MSASLKAKIAEVFDEPGCDKNQAKSEKERKKGCTKQLTPGAAAG
                     GCAFDGAKIALQPIVDVAHLVHGPIACEGNSWDNRHSASSGSTIYRTGFTTDINELDV
                     IYGAEKRLFKAIREICEKVDPPAIFVYQTCV"
BASE COUNT          727 a         1535 c         1448 g          679 t
ORIGIN      
        1 gcgccgcggg tcgagaacga tgagcttggc tccctggcgc aggcgtcgct tgatgcgcga
       61 cgcaagtacc gggtgcgcct cagtggggtt ggcgccgatg accatgaaaa cgtcgacctg
      121 gtccaccgaa cggaagctct gcgtccccgc ggaagtgcca aaggcctggc ccaacgcata
      181 gccggtgggt gcgtggcaca cgcgcgcgca ggtgtcgacg ttgttgttgc cgaaagccgc
      241 acgcaccagc tcctgcacca ggtaagtctc ttcgttggtg cagcgcgacg atgtgatgcc
      301 gcccacggcc tccttgccat aggccgcctg gatgcgtctg aactcggccg ctgcatggcc
      361 aatggcctcg tcccagctca cctcggtcca gggatcggaa gtgctccggc ggatcatcgg
      421 gcgcgtgacg cggtccgggt gggtggcata gccccaggcg aagcggccct tcacgcaggc
      481 gtggccctcg ttggcctggc cgcccttcca tggcgtcatg cgcaccaccc ggtcgtcctt
      541 caactccgcc acgaggccgc aacccacgcc gcaatatgcg caggtggtaa caacgctgcg
      601 ctcgggcgcc ccgcattcga tgacggtctt ctccagcagc gcatcggtcg ggcaggcctg
      661 cacgcaggca ccgcagctca cgcattcact ggccatgaag ggttcgcgcg cgccggccac
      721 gatgcgcgac gcgaagccgc gcccgtcgac ggtgagcgca tagctgccct ggatctcttc
      781 gcaggcccgc acgcagcggc tgcagacaat gcattgggcc gggtcgtaag tgaaataggg
      841 attggattcg tccttggcct cggcgacgtg gctggcgcct tcgtgcccga agcggctgat
      901 ccgcacgccg agctggcgcg ccgtttcgtg cagctcgcaa tcgacatggc cggcgccgct
      961 cacgcagtcc gatgggtggt ccgacaggta cagttccatc acgccgcggc gcagcgcctg
     1021 cagcctgggg ctttgcgtcc tcaccttcat cccggtggcg accggcgtcg tgcacgaggc
     1081 cgggtagcca gcgtgccctt cgatctcgac caggcacagg cgacatgagc cgaaaggttc
     1141 caggctttcg gtggcgcaaa gcttaggcac catcacgccg gcctgcgcgg cggctcgcat
     1201 caccgacgtg cccgcgggca cggtaatcgg gcacccgtcg atctccaacg tgatcacttc
     1261 ggccgacgac gtacgcagcg gcgtgccacg gtcgatgtcg gaagaccggg tcatggtttt
     1321 tcctcacccg gtaggccgaa gtcctgcggg aagtggtcga gcgccgacag cacgggattg
     1381 ggcgtcatac cccccatcgc gcacaggctg gcatgggtca tcgtgtcgca cagctcgcgc
     1441 agcagcacca catgttgcat gcgctcgcgg ccggcgcgga tgcggtcgat gatctcggcg
     1501 ccccgcgtcg agccgatgcg gcaaggcgtg catttgccgc aggactcggc ggcgcagaag
     1561 gccatcgcat ggcgggcgag cttggccatg tcggcccggt cgtcgtgcac gacgatgccc
     1621 ccatgcccga ggctggcacc gatggccgcc gtgtcttcat aggtgagcgg cacgtcccac
     1681 tgcgatggcg gcaggtagct gcccagcggc ccacccagtt gcacggcctt gatcggccgg
     1741 cccgacgcgg tgccgccgcc ccactcgtac agcagttcgc gcaagctctc gccgaagccg
     1801 agctccacca gcccgccgtg gcggatgttg cctgccagct ggaacgcttg cgtgccggtc
     1861 gagcggccgc ggccaagcga acgatagaac tctggcccct tctccagaat ggccggcacg
     1921 gcggccagcg tgagcacgtt gttgacgacg gtgggctgcc cgaagaggcc cgccacggca
     1981 ggcaccggcg gcttgctgcg caccatgccc cgcttgcctt ccagactctc cagcagggcc
     2041 gtctcttcgc cgcacacata cgagccggcg cccatccgca cctggatgtc gatctggcgc
     2101 tgcgagccga gcacggcagg cccgagcatg ccggcttcgc gcgcgcggcg gaccgcctcg
     2161 ccaaacgtgg ccactgcgtc ggggtattcc gagcgaatgt agacgaacgc ctggcgcgcc
     2221 ccagtggcca gcgcggcgat caacaggcct tcgatcagca ggtagggatc gccttcgagc
     2281 agcatgcgat cggcaaaggt gcccgaatcg ccttcgtcgg cgttgcagac gatggtcttg
     2341 cgatctgccc ttgcctgcgc taccgtgcgc catttgatgc cggctggaaa tcctgccccg
     2401 ccgcggccgc gcaacccaga ttcgcccacc tgctggacga cgacttcggg agcgagcgtc
     2461 agggccttgt gcagccccgc ccagcctccg tgggcttcgt agtccgccag cgatagaggg
     2521 tcggtcaagc cggcgtgtgc gaaggtgatg cgccgctgcc gttgcagata tggcaaggcc
     2581 tcgaccaagc ccagcgacgg cgctggtgcg gctgcgccgc gcaacatgcc ggccgccagc
     2641 agcggcgcca cgtcatgctc ggcgaccggt ccatagccta cgcgaccggc cggcgtgtcg
     2701 acctcgacca gcggctccag ccagaacagg ccgcgcgagc tgctgcgcac cagctcgatc
     2761 ggttgccgcg cctgcgcgac cagggcctgc gcgaccgcgt cggcccccac tgcgcgcgcc
     2821 gcgctgtcgc gaggcacgaa gacgcggacg acggtgctca cggcagcaat tccttggccg
     2881 ccagtagcgt gtcgagccgc gcgggcgtca tgcgcgcatg cgcttggcca tcgatggtga
     2941 ccgcgggcga gagcgcgcac agccccaggc aatagaccgg cgtcacggtg tacgcgccgt
     3001 cgccggacgc gggctgagcc acgctgcagc ccagggtagc ccgcgcgtgc atcagcagcg
     3061 cctcgccacc catggcctgg caggactcgg ccgcgcacac ctgcacgacg cagcggcccg
     3121 cgggctgctg ccggaaatgc gagtagtagc cgagcacgcc ctgcacttcg gcgcgcgaga
     3181 ggttcaaggc ctgcgcgatg acaggtaagg cctcgcgcgg cacgaagccc agggcttcct
     3241 gcacgcgatg caggatgggc agcagcgcac cctccaggtg agagagctcc tggacaatgg
     3301 cgcgcacggt cgtcattgcg tcgccagcgg aagtctgggc aacaggcatc ttcggctgct
     3361 ctcggcagtg cttggtcttc gtatgtaaac actatcgccg agcgaggcct tgaccgagcg
     3421 caagccgccg ccttgcgccg ctgccgtcgt ggtcgaacac acccgcatgg cggctcctcg
     3481 cgctcgtcga ggatgcggcg gatgtgctcg gtcgggatga accgcctttg accaccgtga
     3541 cggcccggtc atcccagtgc cacatacgcg acgaactgtg ccgatcacgc agggggcaag
     3601 tcgcgcaggt tgattcgagc gcccgcaagc cgctaggtca cctctgccgc gactcccggc
     3661 cggtccctgc ccaagagtca cacagtggag atgtcgggtc acgttgaagc ccaccaaccc
     3721 gcgatacctg cgcccaagcc caggactcac cggctggcgg gtatcgcgcg ggcaaacctc
     3781 ctcgacgagc gcgacaactc gcgcccccca aagctttgtc gcaaatccgc catttgccgc
     3841 aatggcctgt cgcgccccta cgccttggca gccgacagca aaggcgccaa gtgtttgatt
     3901 ttcaatggat ttgtgccttg gcatcgaagc tgcaatggga cgcacgaact cgccggttcg
     3961 tgtcgccgcc actgcctgag gaggcccaac atgtctgcat cgctcaaagc caagatcgcc
     4021 gaagtctttg atgagcccgg gtgcgataag aaccaggcca agagcgagaa ggaacgcaag
     4081 aagggctgca ccaagcagct gacgcccggc gcggccgccg gcggctgcgc cttcgacggc
     4141 gcgaagatcg cgctgcagcc catcgtggac gtggcgcacc tggtgcatgg cccgatcgcc
     4201 tgcgagggca attcctggga caaccggcac agcgcgtcgt cgggttccac gatctaccgc
     4261 accggcttca ccaccgacat caacgagctc gacgtgatct acggcgccga gaagcgcctg
     4321 ttcaaggcca tccgcgagat ctgcgagaag gtcgacccgc cggccatctt cgtctaccag
     4381 acctgcgtc
//
LOCUS       DASUCY010000340         4101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1549597, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000340 DASUCY010000000
VERSION     DASUCY010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1549597"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="VFV25_06675"
     CDS             complement(<1..751)
                     /locus_tag="VFV25_06675"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828542.1"
                     /note="catalyzes the formation of spermidine from
                     putrescine and S-adenosylmethioninamine; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEY1393041.1"
                     /translation="MTDRAAPPQTTLPSRAGPPARVHPAEVALLASVFIVAACGLVYE
                     LAAGALASYLLGDSVLQFSTVIGTYLFAMGVGSWLSRYFDRQLPAHFLRIELLVALVG
                     GTLPGVLFVANAYIPGAFRLLLYGLVLAVGALVGLEIPLVMRILRRNIVLKELVSQVL
                     TFDYLGALAVSIAFPLLLVPQLGMIRTGLLFGLMNAAVAVWALWLFRHELRRVVSHVV
                     ACGLVLLALVAAFVFAEHITTLAEDKFYQDRV"
     gene            885..1715
                     /locus_tag="VFV25_06680"
     CDS             885..1715
                     /locus_tag="VFV25_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY1393042.1"
                     /translation="MSPPIELYRDPHHACLMFTDLIEEDGRAVQANQFLIVDHGTGAV
                     LDPGGNLAFNEMFLSMSREVAPQRLSYLIASHADPDIIASLDRWLTSTPAKLVISRVW
                     ERFAPHFTKAGKTDGRVIGVPDAGGRLPLGQSELWLLPAHFMHAEGNFHFYDPVSRIL
                     FTGDLAVSLTSGAEAARPVTQLQPHLARMEGFHRRYMVSNKILRLWVGMARQLDIAMI
                     VPQHGAPLEGAAIGEFMDWIENLACGVDLFDDSSYQLPTARIDPRGLHTGPLLRVINS
                     "
     gene            complement(1749..2024)
                     /locus_tag="VFV25_06685"
     CDS             complement(1749..2024)
                     /locus_tag="VFV25_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HEY1393043.1"
                     /translation="MCRNIKTLFNFEPPATELEIRDASLQFVRKLSGFTVPSKANEAA
                     FEKAVEDVAAAARVLIRSMTTSATPRNREVEAAKAHARAEVRFGARD"
     gene            complement(2040..2264)
                     /locus_tag="VFV25_06690"
     CDS             complement(2040..2264)
                     /locus_tag="VFV25_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HEY1393044.1"
                     /translation="MMGIEWLKPEVFFGSILYALVGVVVFWGCFLIVDKLTPYHLWDQ
                     IVDKKNVALAIVVGAMAIAIGLIVAAAIHG"
     gene            complement(2318..3955)
                     /locus_tag="VFV25_06695"
     CDS             complement(2318..3955)
                     /locus_tag="VFV25_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HEY1393045.1"
                     /translation="MATEGQPSPQRVYRAACPNCGAPVEFRSAASPMAVCSFCRSTLL
                     REGEALRRIGVSAELFDDHSPLQLSAGGKYQGAAFTLVGRLQMRYAEGSWNEWHAVFD
                     SGKSGWLSEDNAQFVFSFEAPAPGPLPNAEALRVGAQQVLGREAWTVASVTAVKVGAA
                     EGELPSVPNLARAYVVADLRNTQGEVATIDYSDAAEGKPPRWYLGRGVRLAELAMSGL
                     KDEAAARELKGRSLTCPSCGAALEAKLASTQSIVCHQCKAVVDISQGAGADLSHYAQN
                     VAGASGVEPQIPLGTTGLLALGTAQALPWQVVGYAEHCELDTGDEAEQAFWREYLLYH
                     RSEGFAFLIDSEQGWSWTKPIVGVPQMLGPDRLTLEGVTYRRKYESYASKITYVLGEF
                     YWNLKRDQRSLNTDYEGMGVGEARKQLNREETDGEVTWSAGEMMDAEAVRKAFRLAPE
                     AAAAMARDTGPTSASSSSALAYIFLAMFVLVILFAFVRCSRDDCSQIKASFGEYSNEY
                     QQCLRSGGGASGGYVGGYGGTRGGSFGGYSSGGGGHK"
BASE COUNT          691 a         1422 c         1347 g          641 t
ORIGIN      
        1 ccaccctgtc ctgatagaac ttgtcctccg ccagcgtcgt gatgtgctcg gcaaagacga
       61 acgcggccac gagcgccaga agcaccagcc cgcaagccac cacgtgcgaa acgacccgtc
      121 gcagctcgtg gcgaaacagc cagagtgccc aaacggcaac ggcggcgttc atcagcccga
      181 acaggagtcc cgtgcggatc atgccgagct gcggcaccag caagagcgga aacgcgatcg
      241 acaccgcgag ggcgccgagg tagtcgaagg tcagcacctg cgacaccagt tccttgagca
      301 cgatgttgcg ccggaggatc cgcatcacca gcggaatctc cagcccgacc agcgcgccga
      361 cggccagcac gagcccgtac agcagcagcc ggaaggcgcc cggaatgtac gcattggcga
      421 cgaagagaac gcccggcagc gtgccgccga ccagcgcgac cagcagttcg atgcgcagga
      481 agtgcgccgg cagctggcga tcgaagtagc gcgagagcca ggagccgacc cccatcgcga
      541 acaggtaggt gccgatgacg gtggagaact gcagcaccga gtcaccgagg aggtaactcg
      601 cgagcgcgcc ggccgccagc tcgtagacca ggccgcaggc ggcgacgatg aagaccgagg
      661 cgagaagcgc gacctcggcc gggtgcacgc gtgccggggg cccggcgcgc gaaggcaatg
      721 tcgtctgcgg cggagcggcg cgatcagtca tggcggccga gcgtaccgca gtgcgcggcc
      781 cgcccggcaa gaaccagcga ggcagcctgc attgtcctga gtcaaggcag gccccgggcg
      841 cgcggcctag agtgcggggc tcattttcag ctgcccgccc tctcatgtcg ccgcccatcg
      901 agctctaccg agatccgcac cacgcctgcc tgatgttcac cgacctgatc gaggaggacg
      961 gccgggccgt gcaggccaac cagttcctga tcgtggatca cggaacgggc gccgtgctcg
     1021 acccgggcgg caacctggcc ttcaacgaga tgttcctctc aatgtcgcgc gaggttgcac
     1081 cgcagaggct gtcctatctg atcgcctcgc acgccgaccc ggacatcatc gcctcgctgg
     1141 accgctggct cacatcgacc ccggccaagc tggtgatctc gcgcgtgtgg gagcgcttcg
     1201 cgccgcattt caccaaggcc ggcaagaccg acggccgcgt catcggcgtg cccgatgcag
     1261 gcggccgcct gcccctgggc caaagcgagc tgtggctgct gccggcccac ttcatgcacg
     1321 cggaaggcaa cttccatttc tacgatccgg tcagccgcat cctgtttacc ggcgatctgg
     1381 cggtctccct gacctcgggc gccgaagctg cgcgtccggt cacgcagctg cagccccatc
     1441 ttgcgcgcat ggagggcttc caccggcgct acatggtgtc caacaagatc ctgcgactgt
     1501 gggtgggcat ggcgcgccag ctcgatatcg cgatgatcgt gccccagcac ggcgcgccgc
     1561 tggagggcgc ggccatcggc gagttcatgg attggatcga gaacctggcg tgcggcgtgg
     1621 atctgttcga cgatagcagc taccagctgc ccacggcccg catcgaccct agggggctgc
     1681 acaccggccc gctgctgcgc gtgatcaaca gctgagcggc cgcgctcgac gggcgtagcg
     1741 cgagctcatc aatcgcgtgc accgaagcgg acttcggccc tggcatgcgc cttggccgcc
     1801 tccacttcgc gattgcgcgg ggtggcgctg gtcgtcatcg agcggatcag cacgcgggcc
     1861 gccgcggcga catcctcgac ggccttctcg aaggcagctt cattcgcctt ggacggaacc
     1921 gtgaagccgc tgagcttgcg cacgaactgc agcgaggcgt cgcggatctc cagctcggta
     1981 gccggcggct cgaaattgaa cagggtcttg atgttgcggc acatagggct cagcccctct
     2041 catccatgta tcgccgccgc gacgatcagc ccgatcgcga tcgccatagc gcccacgacg
     2101 atggccagcg ccacgttctt cttgtcgacg atctggtccc acaggtgata gggcgtgagc
     2161 ttgtcgacga tgaggaagca gccccagaac accaccacgc cgaccagggc gtagaggatg
     2221 gagccgaaga agacctcggg tttcagccat tcgatgccca tcatgatgcc gttctccttg
     2281 ctgtgattga ttcatcgaac ctgcactgcc gtcgtcgtca cttgtggcct ccgccgccac
     2341 tgctatagcc gccgaaggag ccgccgcgcg tgccgccgta gccgcccaca tagccgccgc
     2401 tcgcgccgcc gcccgagcgc aggcattgct ggtattcgtt gctgtactcg ccgaagctgg
     2461 ccttgatctg cgaacagtcg tcgcggctgc agcgcacgaa ggcaaacagg atgacgagca
     2521 cgaacatcgc gagaaagatg taggccagcg cgctggatga gctggcggac gtggggccgg
     2581 tgtcgcgcgc catcgcggca gcggcctctg gcgcgaggcg aaaggccttg cgcacggctt
     2641 cagcatccat catctcgccg gccgaccagg tcacctcgcc gtcggtctcc tcgcggttga
     2701 gctgcttgcg cgcttcccca acgcccatgc cttcgtagtc agtgttgagg ctgcgctgat
     2761 cgcgcttcag gttccagtag aactcgccca gcacgtaggt gatcttggag gcgtagctct
     2821 cgtacttcct gcgataggtg acgccttcca gcgtcaggcg atccggcccc agcatctggg
     2881 gcacaccgac gatgggcttg gtccagctcc agccctgctc gctgtcgatc aggaaggcga
     2941 agccctcgct gcggtgatac agcaggtatt cgcgccagaa ggcctgctcg gcttcgtcgc
     3001 ctgtatccag ctcgcaatgc tcggcataac ccaccacctg ccagggcagg gcctgcgcgg
     3061 tgccgagcgc gagcaggccc gtcgtgccca gcgggatctg cggctctaca ccgctggcgc
     3121 cggccacgtt ctgcgcgtag tggctcaagt ccgcccccgc gccctgggag atgtcgacca
     3181 ccgccttgca ctggtggcaa acgatggact gggtgctggc gagcttggcc tccagcgccg
     3241 cgccgcagct cgggcaagtc aggctgcggc ccttgagctc gcgtgccgca gcctcgtcct
     3301 tgaggccgct catcgcgagt tccgccaggc gcacgccgcg gcccaggtac cagcgcggcg
     3361 gcttgccttc ggccgcgtcg ctgtagtcga tggtggcgac ctcgccctgg gtgttgcgca
     3421 gatcggccac cacataggcc cgcgcgagat tgggcacgga aggcagctcg ccctcggccg
     3481 cgccgacctt gacggccgtg accgaggcga cggtccaggc ctcgcgcccc agcacctgct
     3541 gcgcgccgac gcgcaacgct tcggcgttag gcagcgggcc tggcgcgggc gcctcgaagc
     3601 tgaagacgaa ctgggcgttg tcctcgctca gccagccgct cttgccgctg tcgaagacgg
     3661 catgccattc gttccagctg ccctcggcat agcgcatctg cagccggccg acgagcgtga
     3721 aggccgcgcc ctggtatttg ccgccggcac tgagctgcag cgggctgtgg tcatcgaaga
     3781 gctcggcgct gacgccgatg cgtctgagcg cctcgccctc gcgcagcagc gtgctgcggc
     3841 agaagctgca caccgccatc ggcgacgcgg ccgagcggaa ctcgaccggc gcgccgcagt
     3901 tcgggcaggc cgcgcgatag acgcgctgcg gcgaaggctg gccttcggtc gccatgcctc
     3961 agccgcaacg aatctgcaac atggatcccg tccacgcaaa caccgcggat ccggctctgc
     4021 cggtccgctg gtgttgcccc cttgaggggg aggcgcgcag cgccttcggg ggggagccca
     4081 tctctaaacc agcttcttca g
//
LOCUS       DASUCY010000341         3151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_87513, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000341 DASUCY010000000
VERSION     DASUCY010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87513"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /locus_tag="VFV25_06700"
     CDS             complement(<1..1202)
                     /locus_tag="VFV25_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEY1393046.1"
                     /translation="MNAPLPDHIRKALESATLDDKYALESGRAFMSGVQALVRLPMLQ
                     RRRDALAGLNTAGFISGYRGSPLGGYDQALWQAKKHLAANHVVFQPGVNEELGATAVW
                     GTQQLDLYPQTKKYDGVFGIWYGKGPGVDRCSDVFKHANMAGTARHGGVIAIAGDDHV
                     SKSSTAAHQSDHIFKACGLPVFFPSNVQDILDMGLHAFAMSRFSGVWTGMKTIQEVVE
                     SSASVSVDPDRVNIVLPEDFIMPPGGLHIRWPDAPLEQEARLMDYKWYAALAYVRANK
                     LNHNVIAGPNDRFGIIASGKAYNDTRQALLDLGLDDDTCRRIGVRLHKVNVVGPHEAT
                     ITRNVALGLQEILVVEEKRQVIEYQLKEELYGWRPDVRPRVLGKFDEAEGDESGGEWS
                     RPNPSQHWL"
     gene            1411..1959
                     /locus_tag="VFV25_06705"
     CDS             1411..1959
                     /locus_tag="VFV25_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY1393047.1"
                     /translation="MKTRGAAGGEAPPSGEAAAAETHGAELDRYDIAILSELQLDARL
                     SNAELANRIGLSPAPTWRRVKWLEEQGYIRGYRADLDRRRIGLGVLAFVRVDAERNNA
                     EATRALEDAIRALPEVISCHYVSGSGTFELEVLTTDLDAYSRWSMETLFKLPNVKDLH
                     TSFSLGEVKAGSALPLGHLKAR"
     gene            2047..2217
                     /locus_tag="VFV25_06710"
     CDS             2047..2217
                     /locus_tag="VFV25_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393048.1"
                     /translation="MLESYFVGWGTLSLINAGLAQVKGRSGLNWWLLSLLLGPVATLL
                     IVVLQARTGAAD"
     gene            2233..2589
                     /locus_tag="VFV25_06715"
     CDS             2233..2589
                     /locus_tag="VFV25_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEY1393049.1"
                     /translation="MHLLANLLTALVALLHLYILVLEMFLWDKPFGLKTFRQTPERAA
                     QTKVLAANQGLYNGFLAAGLIWGLSLGAAGAPIKVFFLLCVIVAGIYGAATAFRRILF
                     VQALPAAVALGFVLLS"
     gene            2631..>3151
                     /locus_tag="VFV25_06720"
     CDS             2631..>3151
                     /locus_tag="VFV25_06720"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEY1393050.1"
                     /translation="MTASRLLAGASGYAFKEWKGSFYPEKIKPEEMLAWYAERLPTVE
                     INNTFYAMPKPTVLAHWAEVTPAHFVFAIKAPKRITHEARLKADAAADSVAYLYRTLE
                     ALGDKRGPVLFQLPPFLKKDSPRLSEFLSLLPAGHRAAFEFRNDSWFDDEVYALLKGA
                     GAALCLSEREDNAP"
BASE COUNT          492 a         1037 c         1049 g          573 t
ORIGIN      
        1 agccaatgct ggctcgggtt cggccggctc cattcgccgc cgctctcgtc gccttcggcc
       61 tcgtcgaact tgccgagcac gcgcggccgc acgtcgggcc gccagccgta gagctcttcc
      121 ttcagctggt actcgatgac ctggcgcttc tcctcgacga ccaggatctc ctgcagcccg
      181 agcgcgacgt tgcgagtgat ggtggcctcg tgcggcccca ccacgttgac cttgtgcagc
      241 cgcacgccga tgcgccggca ggtgtcgtcg tccagcccca ggtcgagcag cgcctggcgc
      301 gtgtcgttgt aggccttgcc gctggcaatg atgccgaagc ggtcgttcgg gcccgcaatg
      361 acgttgtggt tgagcttgtt ggcgcgcaca taggccagcg ccgcatacca cttgtagtcc
      421 atcagccgtg cttcctgctc cagcggcgca tcgggccagc ggatgtgcag gccgcccggc
      481 ggcatgatga agtcctccgg cagcacgatg ttcacgcggt ccgggtcgac cgagaccgag
      541 gccgaggact cgaccacctc ctgaatggtc ttcatgcccg tccacacgcc cgagaagcgg
      601 ctcatcgcga aggcgtgcag gcccatgtcg aggatgtcct gcacattgga cgggaagaac
      661 accggcaggc cgcaggcctt gaagatgtgg tcgctctggt gcgcggcggt cgagctcttg
      721 ctcacatggt cgtcgcccgc gatcgcgatc acgccgccgt gcctggcggt gccggccatg
      781 ttggcgtgct tgaagacgtc ggagcagcgg tccacgcccg ggcctttgcc gtaccagatg
      841 ccgaacacgc cgtcgtactt cttggtctgc ggatagaggt cgagttgctg cgtgccccac
      901 acggcggtgg cgcccagttc ctcgttgacg ccgggctgga acacgacgtg gttggccgcc
      961 agatgcttct tggcctgcca caacgcctgg tcgtagccgc ccagcggcga gccgcgatag
     1021 cccgagatga agcccgccgt gttgagcccg gccagcgcat cgcgcctgcg ctgcagcatc
     1081 ggcagccgca ccagcgcctg cacgccgctc atgaaggcgc ggccgctctc cagggcgtat
     1141 ttgtcatcca gcgtggcgct ctcgagggcc ttgcggatgt gatcgggcag gggtgcgttc
     1201 atggcatgcg gctccggtcc aaaagaggaa agcggctcgc tcgccggctc gtggccgcgc
     1261 ggcttcgcta gggtctgggg atgctcgcca gtgtaggaaa accgctgctg catgtctttt
     1321 ctttttgtgc caggatccgg gctatcaaag caacgttgtt gcgctgaatt gcctgaaaaa
     1381 ttcagaatgg catcgcccaa gcaaaaacta gtgaaaaccc gtggggctgc cggaggcgag
     1441 gctccgccct cgggtgaggc tgcagctgcc gaaacgcacg gcgccgagct cgaccgctac
     1501 gacatcgcga tcctgagcga gctgcagctg gacgcgcgcc tgtccaacgc cgagctcgcc
     1561 aaccgcatcg gcctgtcgcc cgcgcccacg tggcggcgcg tcaagtggct ggaggagcag
     1621 ggctacatcc gcggttaccg cgccgacctc gaccgccggc gcatcggcct gggcgtgctc
     1681 gccttcgtgc gcgtggacgc cgagcgcaac aacgccgagg cgacgcgcgc gctggaagac
     1741 gcgatccgcg ccctgcccga ggtcatctcc tgccactacg tcagcggctc cggcactttc
     1801 gagctcgagg tgctgaccac cgacctcgac gcctattcgc gctggtcgat ggagacgctc
     1861 ttcaagctgc ccaatgtgaa ggatctacac accagctttt cgctgggcga ggtgaaggcg
     1921 gggagcgcct tgccgctggg gcatctcaag gctcgctgag gcacggtggg gctcgtcggg
     1981 ctcgttgaga atcggggagc ctgcttcgcg caacgcggca aacaacgcat caaggaggac
     2041 tgcaacatgt tggaaagcta tttcgtgggc tggggcaccc tgtccctcat caacgccgga
     2101 ctggcccagg tcaagggccg cagcggcttg aactggtggt tgctgtcgct gctgctgggg
     2161 ccggtcgcaa ccctgctgat cgtggtgctg caagctcgca cgggtgccgc ggactagggg
     2221 cggggatcga ccatgcatct gctcgccaac ctcctcaccg ccctcgtggc gctcttgcac
     2281 ctctacatcc tcgtcttgga gatgttcctg tgggacaagc ccttcggcct gaagaccttt
     2341 cgccagacgc ccgaacgcgc ggcgcagacc aaggtgctgg cggccaacca gggcctgtac
     2401 aacggctttc tggctgccgg gctgatctgg ggcctgagcc tgggcgcggc gggcgcgccg
     2461 atcaaggtct tcttcctgct gtgcgtgatc gtggccggca tctatggggc cgcgacggct
     2521 tttcgccgca tcttgtttgt gcaggccttg ccggctgcag tggcgctggg tttcgtgttg
     2581 ctcagctgaa agcggcgccg ggcgcgcgcc ggtggcacac tgcgcccatc atgacggctt
     2641 cgaggctcct ggcaggcgca agcggctacg ccttcaagga atggaagggc tccttctatc
     2701 ccgagaagat caagccggag gagatgctcg cctggtatgc cgagcggctg ccgaccgtcg
     2761 agatcaacaa caccttctat gcgatgccta agcccacggt gctggcgcac tgggccgagg
     2821 tcacgccggc gcatttcgtg ttcgcgatca aggcgcccaa gcgcatcacg catgaggcgc
     2881 ggctgaaggc cgatgcggcc gccgattcgg tggcctatct ctatcggacg ctcgaagcgc
     2941 tgggcgacaa gcgcggcccg gtgctgttcc agctgccgcc cttcctgaag aaggactcgc
     3001 cgcgcttgag cgaattcctg agcctgctgc cggccggtca ccgcgccgca ttcgagttcc
     3061 gcaacgacag ctggttcgac gacgaggtct atgccttgct gaaaggcgct ggggctgcgc
     3121 tgtgcttgtc cgagcgcgaa gacaacgcac c
//
LOCUS       DASUCY010000342         4073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_392848, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000342 DASUCY010000000
VERSION     DASUCY010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392848"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VFV25_06725"
     CDS             <1..336
                     /locus_tag="VFV25_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEY1393051.1"
                     /translation="AAALMIVTAMFGETFVCMQAFEWTKLIVNEGVRPWGNPMGAAQF
                     GSSFFMITGFHGLHVTAGVIYLMTVAIRLLRGRYDKGGNYQIVEIAGLYWHFVDLVWV
                     FIFALFYLW"
     gene            347..661
                     /locus_tag="VFV25_06730"
     CDS             347..661
                     /locus_tag="VFV25_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HEY1393052.1"
                     /translation="MMSATGQQHPISLYFKIWGLLFVLSTMSYLVDYFHLQGLLRWTL
                     IVVFMLLKAGLIVAVFMHMAWERMALICAILVPPLCILVLVGLMAAEADYTFLTRLLF
                     FR"
     gene            complement(658..1647)
                     /locus_tag="VFV25_06735"
     CDS             complement(658..1647)
                     /locus_tag="VFV25_06735"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEY1393053.1"
                     /translation="MKVCIYGAGAIGGYIGASLALAGVARVSAVARGATLQALRSHGW
                     RLRGSQGEQHVAATASDDPGALGVQDLVVVAVKGPAMAEVARRIAPLLGRDTVVLPAM
                     NGVPWWFGSDLPALGSEPLASVDPGGVIARAIPLKHVIGCVVHIAAATPEPALVEHKM
                     GRGLIIGEPDGSDSARLRSLSGLLAEAGFEVTASPRIRNDVWFKLWGNLTTNPVSALT
                     GATADRIVDDPLVRAFCVAAMSEAAAIGARIGCEVKQTPEERHAVTRKLGAFKTSMLQ
                     DVEAGRPLELDAIVGAVREIGQRVGIATPNIDALLGLTRLFARVQGLYPEATG"
     gene            complement(1814..2008)
                     /locus_tag="VFV25_06740"
     CDS             complement(1814..2008)
                     /locus_tag="VFV25_06740"
                     /inference="COORDINATES: protein motif:HMM:NF023508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3563 family protein"
                     /protein_id="HEY1393054.1"
                     /translation="MNTIFTFFRKLFSADRSTARLTAEQAWVEAYLAQSADIYELESR
                     MRAVELRPTYSSLYPAGLVG"
     gene            2315..2605
                     /gene="groES"
                     /locus_tag="VFV25_06745"
     CDS             2315..2605
                     /gene="groES"
                     /locus_tag="VFV25_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEY1393055.1"
                     /translation="MKLRPLHDRVIVKRVESETKTASGIVIPDNAAEKPDQGEVLAVG
                     PGKRNDKGDFITLNVKVGDRVLFGKYSGQTVKVDGDELLVMREEDLFAVVEK"
     gene            2664..>4073
                     /gene="groL"
                     /locus_tag="VFV25_06750"
     CDS             2664..>4073
                     /gene="groL"
                     /locus_tag="VFV25_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794101.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEY1393056.1"
                     /translation="MAAKDVVFGGEARARMVEGVNILANAVKVTLGPKGRNVVLERSF
                     GAPTVTKDGVSVAKEIELKDKLQNMGAQMVKEVASKTSDNAGDGTTTATVLAQAIVRE
                     GMKFVAAGMNPMDLKRGIDKAVATLVEQLKKASKPTTTSKEIAQVGSISANSDESIGK
                     IIADAMDKVGKEGVITVEDGKSLDNELEVVEGMQFDRGYLSPYFINNPDKQVALLDNP
                     FVLLFDKKISNIRDLLPTLEQVAKAGRPLLIIAEEVEGEALATLVVNTLRGILKVVAV
                     KAPGFGDRRKAMLEDIAILTGGKVIAEEVGLTLEKVSLADLGQAKRVEVGKENTIIID
                     GSGAAADIEARVKQVRVQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGIVAGGGVALLRAKQAAGVIKGDNADQNAGIKIVLRAMEEP
                     LRQIVLNAGEEASVVVAKVI"
BASE COUNT          760 a         1314 c         1335 g          664 t
ORIGIN      
        1 gccgccgcgc tgatgatcgt cacggcgatg ttcggcgaga ccttcgtctg catgcaggcc
       61 ttcgagtgga ccaagctcat cgtcaacgag ggcgtgcggc cctggggcaa tccgatgggt
      121 gcggcccagt tcggctccag cttcttcatg atcaccggct tccacggcct gcatgtcacg
      181 gctggcgtga tctatctgat gacggtggcg atacgcctgc tgcgcggccg ctatgacaag
      241 ggcggcaact atcagatcgt cgagatcgcc gggctgtact ggcacttcgt cgacctggtg
      301 tgggtgttca tctttgcgtt gttctacctg tggtgaggcg gagggcatca tgagcgcaac
      361 gggccagcaa catccgatca gcctttactt caagatctgg ggcctgctgt tcgtgctcag
      421 cacgatgtcc tatctggtgg actatttcca cctccagggt ctgctgcgct ggacgctgat
      481 cgtcgtcttc atgctgctga aggccgggct catcgtcgcc gtcttcatgc acatggcctg
      541 ggagcgcatg gcgctgatct gcgccatcct cgtgccgccg ctgtgcatcc tggtgctggt
      601 ggggctgatg gcggccgagg ccgactacac cttcctcacg cggctgctgt tcttccgtta
      661 gccggtcgct tcgggataga ggccttgcac gcgggcaaac agccgcgtga ggccgagtag
      721 cgcatcgatg ttgggcgtgg cgatgcccac gcgctggccg atctcgcgca ccgcgccgac
      781 gatcgcgtcc agctccagcg gcctgccggc ctccacgtcc tgcagcatcg aggtcttgaa
      841 ggcgccgagc ttgcgcgtca cggcgtggcg ctcctcgggc gtctgcttca cctcgcagcc
      901 gatgcgcgcg ccaattgccg ccgcctcgct catcgccgcc acgcagaagg cacgcaccag
      961 cgggtcgtcg acgatgcggt cggccgtcgc gccggtgagg gccgacaccg ggttggtggt
     1021 caggttgccc cagagcttga accagacgtc gttgcggatg cgcggcgagg ccgtcacctc
     1081 gaagccggct tcggcgagca ggccgctcaa ggaacgcagg cgggcgctgt cgctgccatc
     1141 gggctcgccg atgatgaggc cgcgccccat cttgtgctcg accaacgcgg gctcgggcgt
     1201 agcggcggcg atgtgcacga cgcagccgat cacatgcttc agaggtatgg cgcgggcgat
     1261 gacgccgccc gggtcgacac tggccagcgg ctcgctcccc agcgccggca gatcgctgcc
     1321 gaaccaccag ggcacgccgt tcatggctgg cagcacgacg gtgtcgcggc ccagcagcgg
     1381 cgcgatgcgc cgcgccacct cggccatggc cggacccttg acggcaacga ccaccagatc
     1441 ctgcacgccc agcgcgcccg ggtcatccga ggccgtggcg gcgacgtgct gctcgccctg
     1501 gctgccgcgc aagcgccagc catgcgagcg cagcgcctgc agggtcgcac cgcgcgccac
     1561 cgcgctgacc cgggcgacgc cggccagcgc gaggctcgcg ccgatatagc cgccgatcgc
     1621 gccggcacca tagatacaga ctttcatgct gccccctcgt tcgctcgatt attggagcgg
     1681 accgcgtggg gcgcacgaac ccggatgaaa gccggggttt cagggccgcc tggtcaagct
     1741 agaccccggg gaggccgcgt gcgagcgtcc gacacgccac tcgcggcgca tcagtcagcg
     1801 tcgcgggcaa agctcagccg acgaggccgg ccggatacag cgagctgtag gtcgggcgca
     1861 gttccacggc gcgcatgcgg ctttcgagtt cgtagatgtc ggctgactgg gccagatagg
     1921 cctcgaccca ggcctgttcg gcggtcaggc gggccgtgct gcgatcggcc gagaagagct
     1981 tgcggaagaa ggtgaagatg gtgttcatga tggttcctgc acttgagtgc gataagggtt
     2041 taccctcgat tagctagtat aaaccctagt attgaccctg gctggagtcg gctctccaga
     2101 cgctgagccg gctgccgggc tcccgcagcc gagcggtgtt gcggctccgt cccccttgaa
     2161 tcgtcatgac cggcccctat aattcggccg tgttagcact cgtctcagtc gagtgctagt
     2221 gacttcgctc tgatgatcag cagcaaggtc agtgagcgca aaccatcgct ccacccgcac
     2281 gtcctcaagc aatcgctcta tccaaggaga tgcaatgaaa cttcgtcctt tgcacgatcg
     2341 tgtgatcgtc aagcgcgttg aaagcgaaac caagaccgcc tccggcatcg tgatccccga
     2401 caacgccgcc gagaaacccg atcagggcga ggtgctggcc gtcggtcccg gcaagcgcaa
     2461 cgacaagggc gacttcatca ccctgaacgt caaggtgggc gaccgcgtgc tgttcggcaa
     2521 gtacagcggc cagaccgtca aggtcgatgg cgacgagctg ctggtgatgc gcgaagaaga
     2581 tctcttcgct gtcgtggaga agtaagcctc gcagaggcgc ctctcacccg catcacccca
     2641 atactgaatt cggagaaata gacatggcag caaaagacgt agtgttcggc ggcgaagccc
     2701 gcgcgcgcat ggtcgaaggc gtgaacatcc tggccaatgc ggtcaaggtg accctgggcc
     2761 ccaagggccg caacgtggtg ctcgagcgct ccttcggcgc ccccaccgtg accaaggacg
     2821 gcgtgtccgt ggccaaggaa atcgagctca aggacaagct ccagaacatg ggcgcccaga
     2881 tggtcaagga agttgcttcc aagaccagcg acaacgccgg tgacggcacc accaccgcca
     2941 ccgtgctggc ccaggccatc gtgcgcgaag gcatgaagtt cgtggccgcc ggcatgaacc
     3001 cgatggacct caagcgcggc atcgacaagg cggtcgccac cctggtcgag cagctgaaga
     3061 aggcctccaa gcccaccacc accagcaagg aaatcgcgca ggtcggctcg atctcggcca
     3121 acagcgacga gtcgatcggc aagatcatcg ccgacgcgat ggacaaggtc ggcaaggaag
     3181 gcgtgatcac ggtcgaggac ggcaagagcc tggacaacga gctcgaagtc gtcgaaggca
     3241 tgcaattcga ccgcggctac ctgagcccct acttcatcaa caaccccgac aagcaggtcg
     3301 ccctgctgga caaccccttc gtgctgctgt tcgacaagaa gatcagcaac atccgcgacc
     3361 tgctgcccac gctggagcag gtggccaagg ccggccgtcc gctgctgatc attgccgagg
     3421 aagtcgaggg cgaggcgctc gcgaccctgg tggtcaacac cctgcgcggc atcctgaagg
     3481 tcgtggccgt caaggcgccc ggcttcggcg accgccgcaa ggccatgctg gaagacatcg
     3541 ccatcctgac cggcggcaag gtcatcgctg aagaagtggg cctgacgctc gagaaggttt
     3601 cgctggccga cctgggccag gccaagcgcg tcgaagtggg caaggaaaac accatcatca
     3661 tcgacggttc cggcgccgct gccgacatag aagcgcgcgt caagcaagtg cgcgtgcaga
     3721 tcgaagaagc gaccagcgac tacgaccgcg aaaagctgca agagcgcgtg gccaagctgg
     3781 ccggcggtgt ggccgtgatc aaggtcggcg ctgccaccga agtcgaaatg aaagagaaaa
     3841 aagcccgcgt ggaagacgcc ctgcacgcca cccgcgctgc cgtggaagaa ggcattgtgg
     3901 ccggcggcgg cgtggcgctg ctgcgtgcca agcaagctgc tggcgtgatc aagggcgaca
     3961 acgccgacca gaacgccggt atcaagatcg tgctgcgcgc catggaagag ccgctgcgcc
     4021 agatcgtgct gaacgccggt gaagaagctt cggtggtcgt tgccaaggtg atc
//
LOCUS       DASUCY010000343         2840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1148157, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000343 DASUCY010000000
VERSION     DASUCY010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148157"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..953
                     /locus_tag="VFV25_06755"
     CDS             <1..953
                     /locus_tag="VFV25_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY1393057.1"
                     /translation="KVEHLYVSVWYFVAGLLWIALLYLVAKLPGVHTGVQQATTNWWY
                     GHNALGLWFTPVSVGAIYYFLPKIIGRPVRSYNLSLLGFWTLAFFYGQVGGHHLIGGP
                     IPGWLTTLSIVQSVMMVIPVIAFTINQAGTMWGQMYLARYSPTLRFMMFGGLMYLASS
                     LEGSLESLRSVNAVTHFTHFTVGHAHLGMYAFVAMVMFGAIYFMLPRVLNWEWPYPWL
                     ITLHFWLVAAGIAIYVVALSIGGWLQGEAMLDASRPFMESVALTLPYLKARSLGGALM
                     TLGHLVFVGHFFAMALRFGPQRAGAALFWQQPRLETSHGK"
     gene            943..1533
                     /locus_tag="VFV25_06760"
     CDS             943..1533
                     /locus_tag="VFV25_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit II"
                     /protein_id="HEY1393058.1"
                     /translation="MENEVKLAFGAVISLGIATAALVVLPYIEVSDIKPPTPLKPYSD
                     QQLRGRAVYIANGCVYCHSQQPRDRDLGPDAKRGWGRASVPGDYYYDRPHLLGSMRTG
                     PDLFNIGVRQPSKDWQLGHLYEPRAYVKGSIMPAYPFLFRAKDAPDEGEQVLNLPPGY
                     APPGKVVVATPEALDLVKYLQGLDHTYPVLPPAPKP"
     gene            1687..2292
                     /locus_tag="VFV25_06765"
     CDS             1687..2292
                     /locus_tag="VFV25_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEY1393059.1"
                     /translation="MANERSKSGAQQQREHAEPSERTQPIPIVVAVVTLVMVLFGVAY
                     IFFSESPGDPRLGDRRTVADLRAGEAQPAGAVDGKQIFSANCVACHQATGKGLPGVFP
                     PLDGSEWVNGDERIVANILLHGIDGEISVAGNTFKGSMPSFKQLSDAELAAVASYVRA
                     EWSNKSPAINAELVVQERKANTRTTPFAGGAELKTLVSKTP"
     gene            2309..2767
                     /locus_tag="VFV25_06770"
     CDS             2309..2767
                     /locus_tag="VFV25_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEY1393060.1"
                     /translation="MRLTTMTDYALRLLMYVGQQGERLCTIAEVARVYGISEAHLMKI
                     THQLALAGWLQTVRGKSGGMRLARRPEDINLGAMVRSIEPDFHLVECFSTGSACTLTG
                     HCRLTGVMHEALQGFMAQLDRHTLADILPPTAPKDAPKHAPGKVLKLKVA"
BASE COUNT          446 a          989 c          919 g          486 t
ORIGIN      
        1 gcaaggtcga gcacctctac gtcagcgtct ggtatttcgt tgccggcctg ctgtggatcg
       61 ccttgctcta cctggtggcc aagctgcctg gcgtgcacac cggcgtgcag caggccacga
      121 ccaactggtg gtacggccac aacgcgctgg gcctgtggtt cacgccggtc agcgtgggag
      181 cgatctacta cttcctgccc aagatcatcg gccgcccggt gcgctcctac aacctctcgc
      241 tgctgggctt ctggaccctg gccttcttct acggccaggt cggcgggcat cacctgattg
      301 gcgggccgat accgggctgg ctcaccacct tgtccatcgt gcagagcgtg atgatggtga
      361 tcccggtgat cgccttcacc atcaaccagg ccggcacgat gtgggggcag atgtacctgg
      421 cgcgctattc gccgacgctg cgcttcatga tgttcggcgg cctgatgtac ctggcctcgt
      481 cgctcgaagg ctcgctggag tcgctgcgca gcgtcaacgc cgtcacgcac ttcacgcact
      541 tcaccgtggg ccacgcgcac ctgggcatgt atgccttcgt ggctatggtg atgttcggcg
      601 cgatctattt catgctgccg cgcgtgctca actgggagtg gccgtatccc tggctgatca
      661 cgctgcactt ctggctggtg gcggcaggca tcgccatcta cgtggtcgcg ctgtccatcg
      721 gcggctggct gcagggcgag gccatgctcg atgcgtctcg gcccttcatg gagtcggtgg
      781 ccctgacctt gccttatctg aaggcgcgca gcctgggcgg cgcgctgatg acgctgggtc
      841 acttggtctt cgtcggccat ttcttcgcga tggcgctgcg cttcggcccg cagcgtgccg
      901 gggccgccct gttctggcag cagccgcgtc tggagacgag ccatggaaaa tgaagtcaag
      961 ctcgctttcg gcgccgtcat ctcgctgggc atcgccaccg cggccctggt cgtgctgccc
     1021 tacatcgagg tcagcgacat caagccgccg accccgctca agccctacag cgaccagcag
     1081 ctgcgcgggc gcgccgtcta catcgccaac ggctgcgtct attgccatag ccagcagccg
     1141 cgcgaccgcg acctcgggcc cgatgcgaag cgcggctggg gccgcgcctc ggtgccgggc
     1201 gactactact acgatcgccc gcacctgctg ggctcgatgc gcaccggccc cgacctcttc
     1261 aacatcggcg tgcgccagcc gagcaaggat tggcagctcg gccacctcta cgagccgcgc
     1321 gcctatgtga agggctccat catgccggcc tatcccttcc tgttccgtgc caaggacgcg
     1381 cccgacgagg gcgagcaggt gctcaacctg ccgccgggct atgccccgcc gggcaaggtc
     1441 gtggtcgcca cgccggaggc gctggatctc gtcaaatacc tgcagggcct ggaccacacc
     1501 tacccggtgc tgccgccggc ccccaaaccc tgagcgaggc ggaaatgaac caccccctac
     1561 gctctgacgc gcgccccctt cgagggggcc acgccggcga accggcaaag ccggatccgc
     1621 ggcgtgcgct tggttacgcc ggttcatgcg tcgcgggccg cgcctgggcg cgctggagca
     1681 actgagatgg caaacgaaag aagcaaatcc ggtgcccagc agcagcgcga gcatgcagag
     1741 cccagcgagc gcacccagcc catccccatc gtggtggccg tggtgacgct ggtgatggtc
     1801 ctgttcggcg tggcctatat cttcttttcc gagtcgcccg gcgacccccg gctgggcgac
     1861 cgccgcaccg tggccgacct gcgcgccggg gaggcccagc ccgccggtgc cgtggacggc
     1921 aagcagatct tcagcgccaa ctgcgtggcc tgccaccagg cgaccggcaa gggcctgccc
     1981 ggcgtttttc cgccgctgga tggttcggaa tgggtcaacg gcgacgagcg catcgtcgcc
     2041 aacatcctgc tgcacggcat agacggcgag atcagcgtgg ccggcaacac cttcaagggc
     2101 agcatgccca gcttcaagca gctgagcgat gcggagctgg ccgccgtggc cagctatgtg
     2161 cgcgccgaat ggtccaataa gtcgccggcc atcaatgccg agctggtcgt gcaggagcgc
     2221 aaggccaata cgcgcactac gcccttcgcc ggcggagccg agctgaagac gctcgtttcc
     2281 aagacaccct gagcggagtc ccgatctcat gcgcctgacg acgatgaccg actatgcgct
     2341 gcgcctgctg atgtacgtgg gccagcaggg cgagcggctt tgcaccatcg ccgaggtggc
     2401 gcgcgtctat ggcatctcgg aagcgcatct gatgaagatc acgcatcagc tggcgctggc
     2461 cggctggctg cagacggtgc gcggcaagag cggcggcatg cgcctcgcgc gacgccccga
     2521 ggacatcaac ctcggcgcca tggtgcgcag catcgagcct gacttccatc tggtcgaatg
     2581 cttctcgacc ggcagcgcct gcaccctgac cggccattgc cgcctgaccg gcgtgatgca
     2641 cgaggcgctg cagggcttca tggcccagct cgaccgccac accctggccg acatcctgcc
     2701 gccgaccgca ccaaaggacg caccgaagca cgcgccgggc aaggtgctca agctgaaggt
     2761 ggcatgacat gctgcgcacc gcgctgctca gcctgggcct ggccctgctg ggctatctcg
     2821 acgcggcctg gctgaccgag
//
LOCUS       DASUCY010000344         2521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1437068, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000344 DASUCY010000000
VERSION     DASUCY010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1437068"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VFV25_06775"
     CDS             <1..238
                     /locus_tag="VFV25_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEY1393061.1"
                     /translation="AQCYKPRLQAFEFMLDSLGCNPEDMLHVSSSLRYDLMSANDIGI
                     KNKVFVGRGHGPGCPAYNYTEIKDIGGLAGVVGL"
     gene            374..1675
                     /locus_tag="VFV25_06780"
     CDS             374..1675
                     /locus_tag="VFV25_06780"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEY1393062.1"
                     /translation="MKLDSYWTDSSPAFVPAAGAAGDLPAQVDVAIVGAGFTGLSAAL
                     ALARRGASVAVLEAGPRIAAEASGRNGGHVNNGLAVDYAEVAARVGRERAAAWYRAYD
                     DAVDTVARVVRDEAIACDFMRHGKLKLATRPGQLDALARSAQRLIADGVDTDVEVFDA
                     ASVRAEVRSERFHGGLLYKRSAQMHMGRFAHGLAAAAERRGARIHLDACVNRLERLGG
                     GYAHRLHTARGSVTAQQVLLATGATRHGGYATFGWLRRRIVPIGSFIVVTEPLGADRA
                     NALLAARRTYTTVANIHHYFRLTPDHRLVFGGRARFAVSSPQSDAASGEILRAGLVQT
                     FPQLRDARLDYCWGGLVDMTQDRLPHAGERDGLYYSMGYSGHGTQMSVHMGERMAAVM
                     AGDRDANPWRDRGWPAIPGHFGPPWFLPAVGLYYRLKDKLA"
     gene            1752..>2521
                     /locus_tag="VFV25_06785"
     CDS             1752..>2521
                     /locus_tag="VFV25_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY1393063.1"
                     /translation="MSKSTKLEDFVGPRESQQMMDALQRGATRRDILAMLMAGGMQVG
                     LAGSLAGVAVSAHAQTPKKGGRLRVAGATAAVTDTLDPAKQSNQTDYSRGTMLYNGLT
                     SLDGSLTPQPALAESFTTKDAKTWVFTLRKGVSFHDGKPLSPADVVFSLMRHKDPATG
                     SKAKALADQIDSVKATGPNEVTIQLSAPNADLPVFLGTFHFHIVKDGTTDFSTGIGTG
                     PYKLKEFKPGVRSLMVRNERYWKPGKPYLDEIEFVGIG"
BASE COUNT          405 a          906 c          837 g          373 t
ORIGIN      
        1 ggcacagtgc tacaagccgc gcctgcaggc cttcgagttc atgctggact cgctcggctg
       61 caatccggaa gacatgctgc acgtgtcctc cagcctgcgc tacgacctga tgtcggccaa
      121 tgacatcggc atcaagaaca aggtctttgt cgggcgcggc cacggccccg gctgtccggc
      181 ctacaactac accgagatca aggacatcgg cggcctggcc ggcgtcgtcg gactctgacg
      241 tggaccgccc ctacggcctt cggccgcccc cttcgagggg gcgacaccgg cggaccggca
      301 aagccggatc cgcggtgtcc gcttgggttc ggccgagagc gtcggggttg cgaagcgcgg
      361 tgaagcgact gaaatgaagc tcgattcgta ctggaccgac tcctccccgg cctttgtgcc
      421 ggcagccgga gcggcgggcg atctgccggc ccaggtcgat gtggccatcg tcggtgccgg
      481 cttcaccggt ctgtcggccg ccttggcgct ggcgcgccgc ggcgccagcg tcgcggtgct
      541 ggaggccggc ccccgcatcg ccgccgaggc ctcgggccgt aacggcggcc atgtcaacaa
      601 cggcctggca gtcgactatg ccgaagtcgc cgcgcgggtc ggccgcgagc gcgctgcggc
      661 ctggtatcgc gcctatgacg acgcggtgga caccgtggcg cgcgtcgtgc gcgacgaggc
      721 gatcgcctgc gacttcatgc gccacggcaa gctcaaactc gcgacccgcc ccggccagct
      781 cgatgcgctg gccaggagtg cgcagcggct gatcgccgac ggcgtggaca ccgatgtcga
      841 ggtgttcgat gccgcaagcg tgcgcgccga ggtgcgcagc gagcgcttcc acggcggcct
      901 gctctacaag cgcagcgcgc agatgcacat ggggcgcttc gcgcacggcc tggccgccgc
      961 ggccgagcga cgcggcgcgc gcatccacct ggatgcctgc gtgaaccggc tcgaacgcct
     1021 gggcggcgga tacgcccacc gcctgcacac cgcgcgcggc agcgtcaccg cgcagcaggt
     1081 gctgctggcc accggcgcca cgcgccacgg cggctatgcc accttcggct ggctgcgccg
     1141 gcgcatcgtg ccgatcggca gcttcatcgt cgtcacggaa ccgctggggg ccgatcgggc
     1201 gaacgcgttg ctggccgcgc gccgcaccta caccacggtg gccaacatcc accactactt
     1261 ccgcctcacg ccggatcacc ggctggtgtt cggcgggcgc gcccgcttcg ccgtctccag
     1321 cccgcagtcg gatgcggcga gcggcgagat cctgcgcgcg gggctggtgc agaccttccc
     1381 gcagctgcgc gacgcgcgcc tcgactactg ctggggcggc ctggtcgaca tgacgcagga
     1441 tcgtctgcca cacgccggcg aacgcgacgg cctgtactac tcgatgggct acagcggcca
     1501 cggcacgcag atgtcggtgc acatgggcga gcgcatggcc gccgtgatgg ccggcgaccg
     1561 cgacgccaac ccctggcgcg acaggggctg gccggcgatt cccggccatt tcggcccgcc
     1621 ctggttcctc ccggcagtcg gcctgtacta caggctcaag gacaagctcg cttgagcctg
     1681 atgccggccg ccttttttct ttcaccagcc ctcttttcac cagaccttcc tcacacaacc
     1741 tggagcagcc catgagcaag agcacgaagc tagaggattt cgtcggcccg cgcgaaagcc
     1801 agcagatgat ggatgcgctg cagcgcggcg ccacacgacg cgacatcctc gcgatgttga
     1861 tggccggcgg catgcaagtg ggcctggccg gaagcctggc tggggttgca gtgtccgcgc
     1921 atgcgcagac accgaagaag ggcggccgcc tgcgcgtggc cggcgccacg gccgccgtca
     1981 ccgacacgct ggatcctgcc aagcagtcga accagaccga ctattcgcgc ggcaccatgc
     2041 tctacaacgg gctgacctcg ctggacggca gcctcacgcc gcagcccgcg ctggccgagt
     2101 cgttcaccac caaggacgcc aaaacctggg tattcacgct ccgcaagggc gtgagcttcc
     2161 acgacggcaa gccgctgtcg ccggccgatg tggtgttctc gctgatgcgc cacaaggatc
     2221 ctgcgaccgg ctccaaggcc aaggccctgg cagaccagat cgacagcgtc aaggcgactg
     2281 gccccaacga ggtgacgatt cagctgtccg cccccaacgc cgacctgccg gtgttcctgg
     2341 gcaccttcca cttccacatc gtcaaggacg gcacgacgga cttcagcacc ggcatcggca
     2401 ccggccccta caagctcaag gaattcaagc ccggcgtgcg ctcgctgatg gtgcgcaacg
     2461 agcgctactg gaagcctggc aagccctacc tcgacgagat cgaattcgtc ggcatcggcg
     2521 a
//
LOCUS       DASUCY010000345         4990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1421153, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000345 DASUCY010000000
VERSION     DASUCY010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4990
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1421153"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..718
                     /gene="rdgB"
                     /locus_tag="VFV25_06790"
     CDS             92..718
                     /gene="rdgB"
                     /locus_tag="VFV25_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEY1393064.1"
                     /translation="MRLVLASNNAKKLAELQALFSTQGYELVTQASLGIAEAEEPHVS
                     FVENALAKARHAARASGAAAIADDSGLCVDALRGAPGVASAHFAPVVVPEDAGGREQR
                     RLLQDSANNLLLLQRLRGQSRRSARFISTLVALRHADDPEPLIATGHWRGEILTEPRG
                     SAGFGYDPLVFIPELGATAAELPAQIKAQHSHRALAVREMLALMQAWW"
     gene            720..1940
                     /gene="hemW"
                     /locus_tag="VFV25_06795"
     CDS             720..1940
                     /gene="hemW"
                     /locus_tag="VFV25_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEY1393065.1"
                     /translation="MAQPLWPTAGASSWQPGALNLTSLPPLALYVHLPWCLKKCPYCD
                     FNSHEWGSRAEGGVLPEERYLDALRADLEAALPLVWGRRVISVFIGGGTPSLFSPAGI
                     ERLIADLRARLPLEPGCEITLEANPGTFERERFRGFREAGVTRLSIGVQSFDDAKLKA
                     LGRVHDRAQAMAAIEEARAAFDTYNLDLMYALPGQTLAALEADLKQALAFEPPHLSVY
                     HLTIEPNTFFAKHPPRLPDDDLAFEMLDRITAVTGAAGLERYEISAYAKPGHRCQHNL
                     NYWQFGDYLGIGAGAHSKLSFAHRIVRQVRWREPAAYMSQALEGAAVSNEHEVARADL
                     PFEYMLNALRLKDGFELARFGERTGLAFTAIQPALAQAEARGLMARDLQRAWPTERGF
                     DFLSDLQALFLPGA"
     gene            2104..2451
                     /locus_tag="VFV25_06800"
     CDS             2104..2451
                     /locus_tag="VFV25_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393066.1"
                     /translation="MPAVINPERFFAEARESAMQMPLPGLFADLDVASASVALPDEFL
                     ASPASVQVEVLQQWQRDIERYRRCAIAKMFRDFAALRPELDLLEQLESFRHACEHLGL
                     ECPRDLPLLLQSC"
     gene            2475..4286
                     /locus_tag="VFV25_06805"
     CDS             2475..4286
                     /locus_tag="VFV25_06805"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY1393067.1"
                     /translation="MKSLASRLGMPLDGSLGEQIADEQLSMVLSHTSIATFVATAFAL
                     LLAFYLRGQVEPHLLVLWVVMKVLVAVPRVLQQYAYRREAFASSRGWRQTAYWLLLLD
                     GTVWGLGGFWMMAAPVPTASMVCGSLCCVACLAMFGLQVNARAAAAYVAPIVSLMALG
                     LALRFDAFGTFGAVGLSLFLGLLLMTAARGELLLAEKLMLRMQASRLGEEKAAALRYA
                     QRESASKSQFLGTVTHELRTPVHGVLGVARLVHVESSDPVIRKRMELIETSAAHLLAM
                     VNDLIDISKVGAGAMRLHEAQFDLNEEIARLADIYTVRASEKGLSFTLDNKLPVSCWV
                     GGDPVRTRQVLHNLLGNAIKFTQRGWVQLLVERGEREAQIRFVVRDTGLGIAESQQEA
                     IFEAFAQLNREPGDRPSGAGLGLTISREIARRMGGDITVESRLGFGSIFTFATPLNAA
                     SEPAGLTGAARGGEQLALRSGDGARVLLAEDNEVNALIATSILKGNGYEVERVGDGAQ
                     AVVRALREVDRPALVLMDDSMPVMTGIEATRSIRKQEQALGLARIPILAITAALLDEG
                     LHAYRAAGMDAALAKPFSAAELLEAIEEWVGPRLGTT"
     gene            complement(4181..>4990)
                     /locus_tag="VFV25_06810"
     CDS             complement(4181..>4990)
                     /locus_tag="VFV25_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393068.1"
                     /translation="GEESPMHTQTLSFSLRIARTRADLLAACKVRAESYGHHLPNLRA
                     AIGEPDLLDGDEHTHVVLCVDKISGDPVGTVRVQTNAGGKLLIEHSTSVPEAIASDSR
                     AEITRLAAMPGADPLVKLALMKAAYLYCVAAQVRWMVIGARSEALIRQYRRLGFENLF
                     DDDRRVPLLHAGHLDHQLLKFNVTAAERTWHAGKHALYSFMIETRHPDIDLFTTQPAL
                     ARRETVASRQTRERVAPYVVPSRGPTHSSIASSSSAALNGLASAASMPAAL"
BASE COUNT          757 a         1699 c         1692 g          842 t
ORIGIN      
        1 atggatcgcc tgctcgcgct ggccgacagc ggcatacgca gcctgatcgc tgcgcagaag
       61 gccgcgctgg cagccgattg aggggtcggg catgaggctg gtgctcgcct ccaacaacgc
      121 gaagaagctt gccgaactgc aagccctgtt ctcgacgcaa ggctacgagc tggtgactca
      181 ggcttccttg ggcatcgccg aggcggaaga accgcatgtc agcttcgtcg agaacgcgtt
      241 ggccaaggcg cgccatgcgg cgcgagccag cggcgccgcg gcgatcgccg acgattccgg
      301 gctgtgcgtc gacgcgctgc gcggtgcgcc gggcgtggct tcggcccatt tcgcccccgt
      361 cgtcgtgccc gaggatgccg gcgggcgcga gcagcggcgt ttgctgcagg acagcgccaa
      421 taacctgctg ctgttgcagc gcctgcgcgg gcaaagccgg cgcagcgcgc gctttatcag
      481 caccctggtg gccttgcgcc atgccgatga cccggaaccc ctgatcgcaa ccggacattg
      541 gcgcggcgag atactgaccg agccgcgcgg cagcgcaggc ttcggctacg acccgctggt
      601 cttcattccc gagctcggcg ctaccgcggc cgagctcccg gcgcagatca aggcccagca
      661 cagccatcgc gcactggcgg tgcgcgagat gctggccctc atgcaagcgt ggtggtgacg
      721 tggcccaacc cctatggcct accgccgggg ccagttcttg gcagcccggc gctttgaatc
      781 tcacgagcct gccgccgctg gcgctctatg tgcatctgcc ctggtgcctc aagaaatgcc
      841 cttactgcga cttcaattcg catgagtggg gctccagagc cgagggtggc gtcttgcccg
      901 aggagcgcta tctagacgca ctgcgcgcgg atctggaagc ggcgctgcca ctggtctggg
      961 gccgccgcgt gatctcggtc ttcatcggcg gcggcacgcc cagcctgttc tcgccggccg
     1021 gtatcgagcg cctcatcgcc gacctgcgcg cgcggctgcc gctggagccc ggctgcgaga
     1081 tcacgctgga ggccaatccc ggcaccttcg aacgcgagcg ctttcgcggc tttcgcgagg
     1141 caggcgtgac ccgcctgtcg atcggcgtgc aaagcttcga cgacgccaag ctcaaggccc
     1201 tgggccgcgt gcacgaccgg gcccaggcaa tggcggcgat cgaggaggcc cgggccgcct
     1261 tcgacaccta caacctcgac ctgatgtacg cgctgccggg ccagacgctg gcggcgctgg
     1321 aggccgacct gaagcaggcg ctcgccttcg agccgcctca cctgagcgtc taccacctga
     1381 cgatagagcc caataccttc ttcgccaagc acccacccag gctgccggac gacgatcttg
     1441 ccttcgagat gctcgaccgc atcacggccg tcaccggggc ggccggcctg gagcgctacg
     1501 agatctcggc ctacgcgaag cccggccacc gctgccagca caacctcaac tactggcagt
     1561 ttggcgacta tctgggcata ggtgccggcg cgcattccaa gctcagcttc gcgcatcgca
     1621 tcgtgcgtca ggtgcgctgg cgcgagccgg cggcctatat gagccaggcg ttggagggcg
     1681 cggcggtgtc caacgagcat gaagttgccc gtgccgatct gcctttcgaa tacatgctca
     1741 atgcgctgcg cttgaaggac ggcttcgagc tggcccgctt cggcgagcgc accggcctgg
     1801 ctttcacggc catacagccc gccttggcgc aggcggaggc ccgcggcctg atggcgcgcg
     1861 atctgcagcg ggcctggcct accgagcgcg gcttcgattt tctgagcgac ctgcaggcgc
     1921 tgttcctgcc cggcgcctag agctcgggcg gccaacgccc ttgaatgttg cccaaacacc
     1981 ccgtttcgtg gggtctacag cgtgcatatt gcacggctaa catcgattcc tgcggcgctg
     2041 caggagccac ggcggcttcc gcgcgacaaa cttgctgcct ccggcaagcc aaggagaacg
     2101 accttgccag cagtgatcaa ccccgaacga tttttcgccg aagcgcgtga gtcggcgatg
     2161 cagatgcccc tgccggggtt gtttgccgac ctggacgtgg catcggcttc ggtcgccttg
     2221 cccgacgaat tccttgcttc gcccgcctcg gtgcaggtcg aggtcctgca gcagtggcag
     2281 cgcgacatcg agcgctaccg acgctgcgcc atcgccaaga tgttccgcga cttcgctgcg
     2341 ctgcgccccg agctggatct cctcgagcag ctcgagtcct tccgccatgc ctgcgagcac
     2401 ctgggcctgg agtgcccgcg cgatctgccg ctcctgctgc agagctgctg agcggagcga
     2461 cgatgaacaa gtccatgaaa tccctggcat cgcggctggg catgccgctg gacggcagcc
     2521 tgggggagca gatcgccgac gagcagctga gcatggtgct gtcccacacc tcgatcgcga
     2581 ccttcgtcgc gacggccttt gcgctgctgc ttgccttcta tctgcgcggc caggtcgagc
     2641 ctcacctcct ggtgctgtgg gtcgtgatga aggtgctggt cgccgtgcca cgcgtgctgc
     2701 agcagtacgc ctaccgccgc gaggccttcg ccagcagcag gggctggcgc cagaccgcct
     2761 actggctgct gctgctcgac ggcacggtgt ggggcctggg cggcttctgg atgatggcag
     2821 cccccgtgcc gactgcttcc atggtgtgcg gcagcctgtg ctgcgtggcc tgcctggcga
     2881 tgttcggcct gcaggtcaat gcgcgtgcag cggcggccta cgtggccccc atcgtctcgc
     2941 tgatggcgct gggcctggcc ctgcgcttcg atgccttcgg cactttcggc gccgtcggcc
     3001 tgagcctgtt cctgggcctg ctgctgatga ccgcggcccg cggcgagctg ctgctggccg
     3061 agaagctgat gctgcgcatg caggccagcc gcctcggtga agagaaggcc gccgcactgc
     3121 gctatgcgca gcgcgaatcg gcctccaaga gccagttcct gggcaccgtg acgcacgagt
     3181 tgcgcacgcc ggtgcacggc gtgctggggg tggcgcgcct cgtgcatgtc gaatcgagcg
     3241 atcccgtcat caggaagcgc atggagctga tcgagacctc ggcggcccac ctgctggcca
     3301 tggtcaatga tctgatcgac atctccaagg tcggcgccgg cgccatgcgc ctgcacgagg
     3361 cgcagttcga cctgaacgag gagatcgcac ggctggccga tatctacacc gtgcgggcca
     3421 gtgagaaggg tctgagcttc acgctggaca acaagcttcc cgtgtcctgc tgggtcggtg
     3481 gcgacccggt gcgcacccgc caggtgctgc acaacctcct gggcaacgcg atcaagttca
     3541 cgcagcgggg ctgggtgcag ctgctggtcg agcgcggcga gcgcgaggcg cagatccgct
     3601 tcgtggtgcg cgacacgggc ctgggcatcg cggagagcca gcaggaagcc attttcgagg
     3661 ccttcgcaca gctcaaccgc gaacccggcg accgccccag tggggccggc ctggggctca
     3721 ccatctcgcg cgagatcgcg cggcgcatgg ggggcgacat caccgtggag agccggctcg
     3781 gcttcggctc gatcttcacg tttgcgacac cgctcaatgc tgccagcgag ccggccggcc
     3841 tcaccggcgc cgcgcgcggc ggcgaacaac tggcgctgcg ctcaggggac ggtgcacgcg
     3901 tgctgctggc cgaggacaac gaggtcaacg cgctgatcgc cacctccatc ctgaagggca
     3961 acggctacga agtggagcgc gtcggcgacg gcgcccaggc cgtggtccgc gccttgcgcg
     4021 aggtcgatcg gccggcgctg gtcctgatgg acgattccat gccggtcatg accggcatcg
     4081 aggcgacccg gtcgatccgc aagcaggagc aggccctggg cctggcccgc attcccatcc
     4141 tggccatcac ggccgcactg ctcgacgagg gcctccacgc ctatagggcg gccggcatgg
     4201 acgcggcgct ggccaagccg ttcagcgccg cggagctgct cgaggcgatc gaggaatggg
     4261 tcggcccgcg gctcggcacc acatagggcg cgacccgttc ccgggtctgg cgcgacgcca
     4321 ccgtctccct gcgggcgagc gcgggctggg tcgtgaagag gtcgatgtcg gggtgccgcg
     4381 tctcgatcat gaagctgtag agcgcgtgct tgcctgcatg ccaggtccgc tccgcggcgg
     4441 tgacattgaa cttgagcagc tggtgatcca gatgcccggc atgcagcagc ggtacccggc
     4501 gatcgtcgtc gaacaggttc tcgaagccca ggcgccggta ctggcggatc aaggcctcgc
     4561 tgcgtgcgcc tatgaccatc cagcgtacct gcgcagccac gcaatacagg taggccgcct
     4621 tcatcagggc cagcttgacc agcgggtcgg cgcccggcat cgccgctagg cgtgtgattt
     4681 cggcgcgcga atcgctggcg atcgcctccg gcacggacgt gctgtgctcg atcagcagct
     4741 tgccgccggc attcgtctgc acgcggaccg tgccgaccgg gtcgccgctg atcttgtcga
     4801 cacatagcac cacatgcgtg tgttcatcgc cgtccagcag atcgggctcg cctatggcgg
     4861 cgcgcaggtt cgggagatgg tgtccgtagg actccgcgcg caccttgcag gcagccagta
     4921 gatcggcgcg cgtgcgcgca attctcaagc tgaagctgag cgtttgagtg tgcattggac
     4981 tctcctcgcc
//
LOCUS       DASUCY010000346         2719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_810443, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000346 DASUCY010000000
VERSION     DASUCY010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810443"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="VFV25_06815"
     CDS             <1..868
                     /locus_tag="VFV25_06815"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY1393069.1"
                     /translation="YPKLLSAVPFTPVPGPRLLARDAAARVVLLQAMEAFVRQAGLSS
                     AHALFLDETDRDAAQERGWLIRSSVQFHWSNRENQGYEDFADFLAGLQRDKRKKIQQE
                     RRYVREAGVSFEARMGQEIRAADWDFFYRCYLRTYRAHHSSPYLTRDFFARMATDMTE
                     HWLLFTALRDGKPIASSLIALDPKRRHAYGRYWGALEAVSCLHFEACYYQPLAWCIAN
                     GYQRFEGGAQGEHKMARGLMPVRTWSAHWLSDPRFADAVARHLQREGAGIEAYVDELN
                     ERNPFKGRDGSE"
     gene            966..2312
                     /gene="argG"
                     /locus_tag="VFV25_06820"
     CDS             966..2312
                     /gene="argG"
                     /locus_tag="VFV25_06820"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEY1393070.1"
                     /translation="MATILQHLPAGQKVGIAFSGGLDTSAALHWMKLKGALPYAYTAN
                     LGQPDEPDYDEIPRKAMLYGAQQARLVDCRAQLVAEGIAALQCGAFHISTAGVTYFNT
                     TPIGRAVTGTMLVAAMKEDEVNIWGDGSTFKGNDIERFYRYGLLANPNLKIYKPWLDQ
                     AFIDELGGRAEMSAFMTKAGFGYKMSAEKAYSTDSNLLGATHEAKDLEHLNSGIRIVN
                     PIMGVAFWRDEVEVRREEVTVRFEEGQPVALNGQTFASAVELMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGLALLFIAYERLITGIHNEDTIEQYRDNGRRLGRLLYQG
                     RWFDPQAIMLRETAQRWVARAISGEVTLELRRGNDYSILNTESPNLTYKPERLSMEKV
                     EDAPFSPADRIGQLTMRNLDIVDTREKLATYAKAGLLSPGTGIAMPRLKGSEGDRK"
     gene            complement(2363..>2719)
                     /locus_tag="VFV25_06825"
     CDS             complement(2363..>2719)
                     /locus_tag="VFV25_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY1393071.1"
                     /translation="TFGGNPLAAAVGDAALQLLVDERLSERAASQGAQLMEGLQALRH
                     PAIREIRGRGLLVGVEIDPDFASARVVCEGLMREGVLTKDTHGTVVRLAPPLVIAARE
                     IDEAVAALGRVLKSLG"
BASE COUNT          489 a          944 c          872 g          414 t
ORIGIN      
        1 ctaccccaag ctcttgagcg ccgtgccctt cacgccggtg ccggggccgc gcctgctggc
       61 gcgcgacgcg gccgcgcgcg tggtgctgct ccaggcgatg gaggccttcg ttcgccaggc
      121 ggggctgtca tctgcgcatg cgttgttcct cgatgagacc gaccgcgacg cggcgcagga
      181 gcgcggctgg ctcatccgca gcagcgtgca gttccactgg agcaatcgcg agaaccaggg
      241 ttatgaagac ttcgctgact tccttgccgg gctgcagcgc gacaagcgca agaagatcca
      301 gcaggagcgc cgctatgtgc gcgaagccgg tgtgagcttc gaggcccgca tggggcagga
      361 gatccgcgcg gccgactggg acttcttcta ccgttgctat ctgcgaacct accgtgccca
      421 tcactcctcg ccctatctga cgcgcgactt cttcgcccgc atggccaccg acatgaccga
      481 gcactggctg ctgttcacgg cgctgcggga cggcaagccc attgccagct cgctgatcgc
      541 cctcgatccc aagcgccggc acgcctacgg gcgctactgg ggggcgctcg aagcggtgag
      601 ctgtctgcat ttcgaggcct gctactacca gccgctggcc tggtgcatcg cgaacggcta
      661 tcagcgcttc gaaggcggtg cgcagggcga gcacaagatg gcgcgcgggc tgatgccggt
      721 gcgcacctgg tcggcccatt ggctgagcga cccgcgcttc gccgatgcgg tagcgcgcca
      781 tctgcagcgt gaaggggccg gcatcgaggc ctatgtggac gagctgaacg agcgcaatcc
      841 cttcaagggc cgagacggct cggaatgagg ttccggcgcc gcggatgaag gcgtctcgag
      901 aaagcaccga aaatagcgcg gttcagctcc cagtccagcc cagcccagtt cttacctcac
      961 agaccatggc aaccatcctc cagcacctcc cggccggcca gaaggtcggc atcgccttct
     1021 ccggcggcct cgataccagc gccgcgctgc actggatgaa gctcaagggc gccctgccct
     1081 atgcctacac cgccaacctg ggccagcccg acgagcccga ctacgacgag atcccgcgca
     1141 aggccatgct ctacggggcc cagcaggcgc gccttgtcga ttgccgcgcc cagctcgtgg
     1201 ccgaaggcat cgccgcgctg cagtgcggcg ccttccacat ctcgacggca ggggtgacct
     1261 acttcaacac cacgccgatc ggccgcgccg tcaccggcac catgctggtg gccgcgatga
     1321 aggaggacga agtcaacatc tggggcgacg gcagcacctt caagggcaac gacatcgagc
     1381 gcttctatcg ctacggcctg ctcgcgaacc ccaacctcaa gatctacaag ccctggctgg
     1441 accaggcctt catcgacgaa ctgggcggtc gcgccgagat gtcggccttc atgaccaagg
     1501 ccggcttcgg ctacaagatg tcggccgaga aggcctattc caccgactcc aacctgctcg
     1561 gcgccacgca cgaggccaag gatctggagc acctgaactc gggtatccgc atcgtgaacc
     1621 ccatcatggg cgtggccttc tggcgcgacg aggtggaggt caggcgcgag gaagtcacgg
     1681 tgcgcttcga ggaaggccag ccggtcgccc tcaatggcca gactttcgcc agtgccgtgg
     1741 agctgatgct ggaagcaaac cgcatcggcg ggcggcacgg cctgggcatg agcgaccaga
     1801 tcgagaaccg catcatcgaa gcgaagagcc gcggcatcta cgaggcgcct ggcctcgcac
     1861 tgctcttcat cgcctacgag cgcctgatca ccggcattca caacgaagac accatcgagc
     1921 aataccgcga caacggccgt cgcctgggcc gcctgctcta ccagggccgc tggttcgatc
     1981 cgcaggccat catgctgcgc gaaacggccc agcgttgggt ggcgcgagcg atcagcggcg
     2041 aggtcacact ggaactgcgt cgcggcaacg actactcgat cctgaacacc gagtcgccca
     2101 acctcaccta caagcccgag cggctgtcga tggagaaggt ggaagacgcc cccttctcgc
     2161 ccgccgaccg catcggccag ctaaccatgc gcaatctcga catcgtcgac acgcgcgaga
     2221 agctggcgac ctatgccaag gccggcctgc tgagccccgg cacgggcatc gcgatgccgc
     2281 gcctgaaggg cagcgagggc gacaggaagt aaggcacggc ggccttcgcc tgcgcgacgg
     2341 ccgccgctct accggtgctg gttcagccca gcgatttcag cacccgcccg agcgccgcga
     2401 ctgcctcatc aatctcgcgt gctgcgatga cgagcggcgg cgccaggcgc accacggtgc
     2461 cgtgggtgtc cttggtgagc acgccttcgc gcatcaggcc ttcgcatacg acgcgcgcgg
     2521 aagcaaagtc ggggtcgatt tctacgccga ccaacagccc gcggccgcgt atctcgcgga
     2581 tggccggatg cctgagggct tgcaggccct ccatcagctg agcgccctgg ctggccgcac
     2641 gctctgacag gcgctcgtcg accagcaatt gcagggccgc atcgccgacc gcggcagcga
     2701 gcgggttgcc gccgaaggt
//
LOCUS       DASUCY010000347         2803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1131935, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000347 DASUCY010000000
VERSION     DASUCY010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131935"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..806
                     /locus_tag="VFV25_06830"
     CDS             153..806
                     /locus_tag="VFV25_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEY1393072.1"
                     /translation="MNVEQARFNMIEQQIRPWDVLDTSVLSLLAIVKREDFVPAAQRA
                     LAFMDLEVPLADGQAMLSPKVEARMLQELAPARHELVLEVGTGSGYMAALLAHKAQRV
                     VSLEIRPELAAFARQNLQRAAVLNVEVREADGAKGYAAEAPFDAIVLSGSVAEVPQAL
                     LDQLKIGGRLIAIVGEEPVMRATLVTRVGEHEFRSVDLFDTVAPRLEHFPEPSKFSF"
     gene            818..1156
                     /locus_tag="VFV25_06835"
     CDS             818..1156
                     /locus_tag="VFV25_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY1393073.1"
                     /translation="MEQVEVYEVAALIASFGPEAQPLVLDVREPWELQRAALSVPGTR
                     TVHIPMNELPRRLGELDSSQPILALCHHGARSLQVVAFLLRAGYERVYNLAGGIDAWS
                     REIDASVPLY"
     gene            1193..2587
                     /locus_tag="VFV25_06840"
     CDS             1193..2587
                     /locus_tag="VFV25_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HEY1393074.1"
                     /translation="MPAASATPSLGLKQLALACALSLGAAAPYAQAQSLSELYQAARG
                     YDASYLAARALADSAQYKADQAHGLRRPTVNATVGATRNVNDQPSAAILGTPPQVVQY
                     SSNTTVRTTSAGVAGTWALLNRANDATIAQADKGLESAQADLQVAEQDLIVRLAQTYF
                     DVLNAQDTLATAQANKKAIAEQLASAKRNFEVGNATITDTREAQARFDLATAQEIAAD
                     NDLHTKRIALDQLVGRSDVSPKPLAAPVALPPLMPAGVEEWVAQTDQAPSVRKARVGY
                     EVAQLETEKARAGHLPTLNLNAGYTKGHSNIDQSAFGQSTGLSGPVTQSNIGITLNVP
                     LFAGYTVQNRVKETLLLEEQTRNNLEAARRGTAQATRQAFFGVQSGQAQVQALEAAES
                     SSKLALEATELGYKVGVRVNIDVLNAQTQLYTTERDLAKARYDVIVNSLKLKQAAGTL
                     QPDDIEASNRLLAQ"
     gene            complement(2591..>2803)
                     /locus_tag="VFV25_06845"
     CDS             complement(2591..>2803)
                     /locus_tag="VFV25_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008342494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HEY1393075.1"
                     /translation="AELSIAAGAALQVEDWPQALAQALHLLGDEPARLRMVTAARDFA
                     AAHRGAAARMAELVLAELDAPPGASS"
BASE COUNT          489 a         1011 c          909 g          394 t
ORIGIN      
        1 ggggtgatca cctcgtcgct gctgacgctg gttgtcgtgc ctgtcatcta ttgctacctc
       61 gacgacctcg ccgcgtgggc gaaaaggaag tgggcggccc ctaaaatcac gggtttgccc
      121 caaccccatc ccgtctccgg aggaacgccg ccatgaacgt cgaacaagcc cgcttcaaca
      181 tgatcgagca gcagatccgc ccctgggacg tgctcgacac ttcggtgcta tcgctgctgg
      241 ccatcgtcaa gcgcgaggac ttcgtgcctg ccgcccagcg cgcgctggcc ttcatggatc
      301 tcgaagtgcc cctggcggat ggccaggcca tgctctcgcc caaggtcgag gcgcgcatgc
      361 tgcaggaact ggccccggcc cggcacgagc tggtgctcga agtcggcacc ggcagcggct
      421 acatggccgc gctgctggcg cacaaggcgc agcgcgtcgt gtcgctggag atccgccccg
      481 agctcgctgc tttcgcgcgc cagaacctgc agcgcgccgc cgtgctgaac gtcgaggtgc
      541 gcgaggccga cggagccaag ggctatgcgg ccgaggcgcc cttcgatgcg atcgtgctgt
      601 ccggctcggt ggccgaggtg ccgcaggcgc tgctcgacca gctcaagatc ggcggccggc
      661 tgatcgccat cgtcggcgag gagccggtga tgcgcgcaac gctcgtcacg cgtgtgggcg
      721 agcacgagtt ccgcagcgtc gacctgttcg acacggtggc gccgcgcctc gagcacttcc
      781 ccgagcccag caagttcagc ttctgagcgc gccgcccatg gagcaggtcg aggtctacga
      841 agtcgccgcg ctgatcgcct ctttcgggcc tgaagcgcag ccgctggtcc tcgacgtccg
      901 cgagccctgg gaactgcagc gcgccgcgct cagcgtgccg ggcacgcgca cggtccacat
      961 cccgatgaac gagctgccca ggcgcctggg cgagctcgat tcttcgcagc ctatcctcgc
     1021 tttatgtcat cacggagccc gcagcctgca ggtcgtcgca ttcctgctgc gcgcgggcta
     1081 tgagcgcgtg tacaacctcg ccggcggcat cgatgcctgg tcccgcgaga tcgatgcctc
     1141 cgtgcctctg tactgacatt cccgccaaca agaagctgaa caaggaagac ccatgcccgc
     1201 tgcgtccgcg acaccctcgc ttggtctcaa gcaattggcg ctggcctgcg ccctgagcct
     1261 gggcgccgcc gccccttacg cacaggcgca aagcctgagc gagctctacc aggcggcgcg
     1321 cggctatgac gcaagctacc tcgcggcacg agcgttggcc gattcggccc agtacaaggc
     1381 cgaccaggcc cacggcctgc gccgccctac cgtcaacgcc acggtcggcg cgacgcgcaa
     1441 cgtgaacgac caacccagtg cggcgatcct gggcacgccg ccacaggtcg tgcagtacag
     1501 cagcaatacg acggtacgca ccacctcggc gggcgtggcg gggacgtggg cgctgctcaa
     1561 ccgagccaac gacgcaacca tcgcccaagc cgacaaggga ctggaatcgg cgcaggccga
     1621 tctgcaggtc gccgagcagg acctgatcgt gcgcctggcg cagacctatt tcgacgtgct
     1681 caatgcccag gacacgctgg ccacggccca ggccaacaag aaggccatcg ccgagcagct
     1741 cgcctccgcc aagcgcaact tcgaggtcgg caacgccacc atcaccgaca cgcgcgaggc
     1801 ccaggcccgc ttcgacctcg cgaccgcgca agaaatcgcg gccgacaacg acctgcacac
     1861 caagcgcatt gcgctcgacc agctggtcgg ccgcagcgac gtaagcccca agccgctcgc
     1921 cgcgccggtg gcgctgccgc ccctgatgcc ggccggcgtc gaggaatggg tcgcgcagac
     1981 cgatcaggca cccagcgtgc gcaaggcgcg cgtcggctac gaggtcgccc agctggagac
     2041 cgagaaggcg cgcgccggcc atctgccgac gctcaacctg aacgcgggct acacgaaggg
     2101 ccattccaat atcgaccaga gcgccttcgg tcagagcacg ggcctgtctg gccccgtgac
     2161 gcagtccaac atcggcatca cactcaatgt gccgctgttc gccggctaca cggtgcagaa
     2221 ccgcgtcaag gagaccctgt tgctcgagga gcagacgcgc aacaacctgg aggcggcccg
     2281 ccgcggcacg gcccaggcga cgcgccaggc cttcttcggc gtgcagtccg gccaggccca
     2341 ggtgcaggca ctggaggcgg ctgaatcgtc gagcaagctg gcgctggagg cgaccgagct
     2401 cggctacaag gtgggcgtgc gcgtcaacat cgacgtgctc aacgcccaga cccagctcta
     2461 caccaccgag cgcgacctcg ccaaggcgcg ctatgacgtc atcgtcaaca gcctgaaact
     2521 caagcaggcg gccggcacgc tgcagcccga cgacatcgag gcgtcgaacc gcctgctggc
     2581 ccagtaagct ttagctggat gcgccgggcg gcgcgtccag ctcggccagc acgagttccg
     2641 ccatgcgcgc ggccgcgccg cgatgggccg cggcgaagtc cctggctgcg gtgaccatgc
     2701 gcagccgtgc aggctcatcc ccgagcagat gcagggcctg cgccagggct tgcggccaat
     2761 cctccacctg gagcgcagca cctgcggcga tggagagctc ggc
//
LOCUS       DASUCY010000348         2314 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_906855, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000348 DASUCY010000000
VERSION     DASUCY010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_906855"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="VFV25_06850"
     CDS             complement(<1..650)
                     /locus_tag="VFV25_06850"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY1393076.1"
                     /translation="MPVFLRRILAFCAMALVAAGCARAADYPSHPVRVLVGYAPGGTT
                     DFSGRLIAQALSAQLKQPFVVENMVGASGTKAAEEVVHSAPDGYALYVADSTFSMLPW
                     VYGQLKWKPESDLKLAAMIMQAPAVLVVAPGSPFKSVREIIEHARQHPDQLSYGSAGV
                     GTSVHLYAELFQQQAKIKLVHREFNGSGEAVKAVQSGKVDLMFTALPSALKSLKEG"
     gene            complement(782..1261)
                     /locus_tag="VFV25_06855"
     CDS             complement(782..1261)
                     /locus_tag="VFV25_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEY1393077.1"
                     /translation="MTSTTSPTTKPPRYYWEDFPVGSTRVFGGHTLGKEEMLRFAREF
                     DPQPFHVDEELAKHSLFGALAASGWHSCALVMRMMCDEYLLDSSSLGSPGLEHIKWLK
                     PVLVGDTLRVRMEVLEARVMNSRPSVGLVRSSWTALNQRDEAVLSMDGWGMFRRREA"
     gene            1306..2172
                     /locus_tag="VFV25_06860"
     CDS             1306..2172
                     /locus_tag="VFV25_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007589992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEY1393078.1"
                     /translation="MKVLIPLPRRDFDPTEAAVSWKILRDASHDISFATPDGRPAAAD
                     PLMLSGEGLDPWGRIPLLKKLRLVGLVLRANAGARAAYREMQRDARFLAPLRYEQAAV
                     ADFDGLLLPGGHAKGMREYLESETLQALVAAFFDAGKPVAAVCHGVVLAARSVSPRSG
                     RSALHGRKTTALTWAQESKAWTLARYWARPWEPGYYRTYEELPGEPPGYRGVQREIER
                     ALANPADFLDVPRNAANYMAKTSGLQRDSLHDERPAWVVRDGHYVSARWPGDVHSFAK
                     TFDRVLREYAAR"
BASE COUNT          375 a          805 c          780 g          354 t
ORIGIN      
        1 ttgccctcct tcagcgactt gagcgccgag ggcagggccg tgaacatcag gtccaccttg
       61 cccgactgca cggccttgac cgcctcgccc gagccgttga actcgcggtg cacgagcttg
      121 atcttggcct gctgctggaa cagctcggca tagagatgga ccgaggtgcc gacgccggcc
      181 gagccgtagc tgagttggtc cgggtgctgg cgcgcatgtt cgatgatctc gcgcaccgac
      241 ttgaacggcg agcccggcgc cacgaccagc accgccggcg cctgcatgat catcgcggcc
      301 agcttcaggt cgctctcggg cttccacttc agctggccat agacccaggg cagcatcgag
      361 aaggtgctgt cggccacgta gagcgcgtag ccgtcgggcg cggagtgcac cacttcctcg
      421 gccgccttcg tgcccgaggc acccaccatg ttctcgacga cgaagggctg cttcagctgg
      481 gccgacaagg cctgggcgat caggcggccc gagaagtccg tggtgccgcc cggcgcatag
      541 ccgaccagca cccggaccgg atggctcgga tagtcggccg cccgggcgca gcccgcggcg
      601 acgagcgcca tggcgcagaa ggcaaggatg cggcgaagga agacaggcat gggctcgacc
      661 tcgtgctggg agctgcgcag cctaacgagc cgcatcacgg gcggccattg tggataaccg
      721 agtccaggaa agcccgcagt gccaggcctg cagttcgccg ctttgttgca gccgggtcag
      781 gtcatgcctc acggcgccgg aacatgcccc agccgtccat gctgagcacg gcctcgtcgc
      841 gctggttgag cgccgtccag ctcgaacgca ccaggcccac gctgggccgg ctgttcatca
      901 cgcgggcctc cagcacctcc atgcgcacgc gcagcgtgtc gcccaccagc accggcttca
      961 gccacttgat gtgctccagc cccggcgagc ccaggctgct cgaatcgagc aggtattcgt
     1021 cgcacatcat gcgcatcacg agcgcgcagc tgtgccagcc gctggccgcc agcgcaccga
     1081 acagcgaatg cttggcaagc tcttcatcga cgtgaaaggg ctggggatcg aactcgcgcg
     1141 cgaagcgcag catctcttcc ttgccgaggg tatgcccgcc gaagacgcgg gtgctgccaa
     1201 cggggaagtc ctcccagtag tagcgcgggg gtttggtggt tggcgaggtc gtgctggtca
     1261 tggaaaactc cgggcgtcga ttgtggcggc accccggagt gaagcatgaa agtcctgatt
     1321 cccctgccgc gccgggactt cgaccccacc gaggctgcgg tgagctggaa gatcctgcgc
     1381 gatgccagcc acgacatcag cttcgcaacg cccgacggcc ggcccgcggc ggccgatccg
     1441 ctgatgctca gcggtgaggg cctggacccc tggggccgca tccccttgct gaagaaactg
     1501 cgcctcgtgg gcctggtgct gcgcgccaat gccggtgcgc gcgctgctta tcgcgagatg
     1561 cagcgtgatg cacgcttcct ggctccactg cgctacgaac aggccgccgt cgcggatttc
     1621 gacggcctgc tgctgcccgg tggccacgcc aagggcatgc gcgagtacct ggagagcgag
     1681 acgctgcagg ccctggtcgc cgccttcttc gacgcgggca agcccgtggc cgcggtctgc
     1741 cacggcgtgg tgctggcggc gcgcagcgtc tcgccgcgca gcggccgatc ggcgctgcac
     1801 ggccgcaaga ccacggccct cacctgggcg caggagagca aggcctggac gctggcccga
     1861 tactgggccc gcccctggga gccgggctac taccgcacct acgaagagct gcccggcgag
     1921 ccgcccggtt atcgcggcgt gcaacgcgaa atcgagcgag cgctggcgaa cccggcggac
     1981 tttctggacg tgccgcgcaa tgcggcgaac tacatggcca agaccagcgg actgcaacgc
     2041 gattcattgc acgacgagcg gcccgcctgg gtcgtgcgcg acggccacta tgtctcggcg
     2101 cgctggcccg gcgacgtgca cagcttcgcg aagaccttcg atcgcgtgct gcgcgaatac
     2161 gccgcccgct aagcggctca agcgctgggc ttgcccggct tgaacaggcc gtcgcaggtg
     2221 gcgcgctgtg gatcctgcgc ctccagcctg ccgcacacac cgctcagctg gctctgcaat
     2281 cgctgcagca ctgcagcatg ccggccatcg cgat
//
LOCUS       DASUCY010000349         2281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_842926, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000349 DASUCY010000000
VERSION     DASUCY010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842926"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1277)
                     /gene="tssF"
                     /locus_tag="VFV25_06865"
     CDS             complement(<1..1277)
                     /gene="tssF"
                     /locus_tag="VFV25_06865"
                     /inference="COORDINATES: protein motif:HMM:NF017733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HEY1393079.1"
                     /translation="MDPRLLRFYADELTHLREVGAEFAEEFPKIAARLGMEGAEVADP
                     YVERLLEGFAFLAARVQLKLEAEQPRLIAHLLEAIYPNFLAPVPSMMVARLAVDTADP
                     NLVKGYAVPRGSAVISELARGQDTHCEFRTAHEVRLWPVELASVQYFSYAPDLPLARL
                     PQAQGAKGGLRVRLRTGGGLGLDQLGLDRLAFYISAPDDVAFRLHELVLGTTLGSWVA
                     TEAGRATEQWRDAGSVQPLGFADTEALLPETLRVFSGYRLLQEMAAMPQRLLFFELRD
                     LAPRLAQVKGEEVELVIFFARGDAALEALVDAKSLALHCTPAINLFHKRLDRIQLGAG
                     GWEYHVVPDRTRPMDYEVHNLASVVGFGTGRVAQQEFLPLYATYHEAPRDNPGYYTVR
                     REPRLLSPRQKQQGPRSSYIGEEVFVSLVDPRHA"
     gene            complement(1277..1801)
                     /gene="tssE"
                     /locus_tag="VFV25_06870"
     CDS             complement(1277..1801)
                     /gene="tssE"
                     /locus_tag="VFV25_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HEY1393080.1"
                     /translation="MSIQDRLQPALLDRLTDDSRHQPTEGDEQRVMSKSQLRQAVLRD
                     LGWLFNAIQPLYPLAETSSAVLADCVLNFGLPPLAGQLASKVDIDFMERAIKQAIVRF
                     EPRILPATLEVRALEASSVLDTHNIIEFEIRGQLWSQPVPLEILLRTQLDLEAGQVEV
                     RDALALPSSRPRGR"
     gene            complement(1798..>2281)
                     /locus_tag="VFV25_06875"
     CDS             complement(1798..>2281)
                     /locus_tag="VFV25_06875"
                     /inference="COORDINATES: protein motif:HMM:NF018699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system accessory protein TagJ"
                     /protein_id="HEY1393081.1"
                     /translation="EALRKQAMEQAEPSPGRVDGEAVQWICDADSRLGPVLEAFVNGR
                     YCWLPFGAIARLQIEAPQDLRDLVWASAHLEFPNGGEAVALIPARYPGCGPDNDERLL
                     MSRLTEWEVLSGEPGEPGEHYAGRGQRILATDLGETGLLELRSIELTAQAGQHEADAA
                     "
BASE COUNT          349 a          798 c          799 g          335 t
ORIGIN      
        1 gcgtgccggg gatcgaccag cgagacgaac acctcctcgc cgatgtagga cgagcgcggc
       61 ccctgctgct tctggcgcgg cgagagcagg cggggctcgc gcctcacggt gtagtagccc
      121 gggttgtcgc gcggcgcttc gtgataggtg gcatacagcg gcaggaattc ctgctgcgcc
      181 acgcggccgg tgccgaagcc gaccacgctc gccaggttgt gcacctcgta gtccataggc
      241 cgcgtgcggt ccggcaccac gtggtattcc cagccgcccg ccccgagctg gatgcggtct
      301 agccgcttgt ggaagaggtt gatggcgggc gtgcagtgca gcgccaggct cttcgcgtcc
      361 accagcgctt ccagcgcggc atcgccgcgc gcgaagaaga tcaccagttc gacctcctcg
      421 cccttgacct gcgccagccg cggggccagg tcgcgcagct cgaagaacag caggcgctgc
      481 ggcatggccg ccatctcctg cagcaggcga tagccggaga agactcgcag cgtctcgggc
      541 aacagggctt cggtatcggc aaagcccagg ggttgcacgc tgcccgcatc gcgccattgc
      601 tcggtggccc gtccggcttc ggtggcgacc cagcttccca gcgtcgtccc cagcaccagc
      661 tcgtgcaggc ggaaggccac gtcgtcgggc gcgctgatgt agaaggccag gcggtccagg
      721 cccagctggt ctagccccaa gccgccgccg gtgcgcaggc gcacgcgcag gccacccttg
      781 gcgccctggg cctggggcag ccgcgccagc gggagatcgg gcgcgtagct gaaatactgg
      841 acgctggcca gctcgacggg ccacagcctc acttcgtgcg cggtgcggaa ttcgcaatgc
      901 gtgtcctggc cgcgcgccag ctccgagatc acggcgctgc cgcgcggcac cgcgtagccc
      961 ttgacgaggt tgggatcggc cgtgtccacc gccaggcgtg cgaccatcat cgacggcacg
     1021 ggcgccagga agttgggata gatcgcctcc agcagatgcg caatcaggcg cggctgctcc
     1081 gcctccagct tgagctgcac ccgggccgcc aggaaggcga agccctccag caggcgctcg
     1141 acatagggat cggcgacctc ggccccctcc atgcccaggc gggccgcgat cttcgggaat
     1201 tcctcggcga attcggcgcc gacctcgcgc agatgcgtaa gctcgtcggc gtagaagcgc
     1261 agcaggcgcg ggtccatcag cgcccccgcg gccgcgacga cggcaaggcc agcgcgtcgc
     1321 gcacctccac ctgcccggcc tccagatcga gctgggtgcg cagcaggatc tccagcggca
     1381 ccggctgcga ccagagctgg ccacggatct cgaactcgat gatgttgtgg gtgtccagca
     1441 cgctgctggc ctccagcgcg cgcacttcca gcgtcgcggg caggatgcgc ggctcgaagc
     1501 ggacgatggc ctgcttgatg gcgcgctcca tgaaatcgat gtcgaccttg gatgcgagct
     1561 ggcccgccag cggcggcagg ccgaaattga gcacgcagtc cgccagcacc gcagagctgg
     1621 tctcggccag cggatagagc ggctggatgg cgttgaagag ccaaccgagg tcgcgcagca
     1681 ccgcctggcg cagctgcgac ttggacatca cgcgctgctc gtcgccctcc gtcggctggt
     1741 ggcgcgaatc gtcggtcagg cggtccagca aggcgggctg caggcgatct tgaatgctca
     1801 tgctgcatcc gcttcgtgct gcccggcttg cgcggtcagc tcgatgctgc gcagctccag
     1861 caggccggtc tcgcccagat cggtcgccag gatgcgctgg ccgcgcccgg cgtaatgctc
     1921 gccgggctcg ccgggctcgc cggacaagac ctcccattcg gtcaggcgag acatcaggag
     1981 gcgctcgtcg ttgtcggggc cgcagccggg gtagcgcgcc gggatcaggg ccaccgcttc
     2041 gccgccgttg ggaaactcca ggtgagcgct cgcccagacc aggtcgcgca ggtcctgcgg
     2101 ggcctcgatc tgcagccggg cgatggcgcc gaatggcagc cagcaatagc gcccgttgac
     2161 aaaagcctcc agcaccggac cgaggcgcga atcggcgtcg cagatccatt gcacggcctc
     2221 gccgtcgacg cggccggggc tgggctcggc ctgctccatg gcctgcttgc gcagggcctc
     2281 g
//
LOCUS       DASUCY010000350         3967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1453262, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000350 DASUCY010000000
VERSION     DASUCY010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1453262"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VFV25_06880"
     CDS             complement(<1..352)
                     /locus_tag="VFV25_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010090556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
                     /protein_id="HEY1393082.1"
                     /translation="MPIRPTPANQRTALQVHLGTKGTEVGELVFVQQGRREFSQFQYV
                     TAWLADANRFEVSPDLPLVAGFQNRKAPTAHDSVFHLAIADTAPDAWGRRVVDRARAK
                     DRRLNPKLGPLTELD"
     gene            complement(364..765)
                     /locus_tag="VFV25_06885"
     CDS             complement(364..765)
                     /locus_tag="VFV25_06885"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY1393083.1"
                     /translation="MAVPLCGRIIDLMGTAKNRKRSDEGSTPARPLPQPAQRAITQLA
                     LDFSRARRRRAMTQADLALRAGVSLSTIRRLEAGDSRMQVHVLARVLLVFGELDKLKA
                     LLDSATDDIGLALADEGLPQRVRARKGQNAF"
     gene            complement(1087..2097)
                     /locus_tag="VFV25_06890"
     CDS             complement(1087..2097)
                     /locus_tag="VFV25_06890"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEY1393084.1"
                     /translation="MSNHDDPSRPEPTFGAPPPPAPAAAPDLPATSWGVVEEFARSYL
                     ADRRSERRWRIFFRLAWLLLFAAIVFAAVNERNLANAPSGPHTALIEVHGEIAADAES
                     SAENVITALRNAFDASDAKGVVLRINSPGGSPVQAGLINDEIRRLRALHKDRKLYAVC
                     EEMCASAAYYIAVGADEVYVDKASLVGSIGVLMDGFGFVGLMDKLGIERRLLTAGDNK
                     GMLDPFSPQNDKQRAYAQAMIDQIHQQFISVVKQGRGKRLKQTPETFSGLFWNGQQAI
                     DQGLADHLGSLDYIAREVVKAEDVVDYTTKENVAERLAKRFGASIGAGAVHAMQAMGG
                     LH"
     gene            complement(2153..2542)
                     /locus_tag="VFV25_06895"
     CDS             complement(2153..2542)
                     /locus_tag="VFV25_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEY1393085.1"
                     /translation="MLETQASQGVALCTSADLAERGPAQVFDVLEYGRSVRAFALRFE
                     GRVVAYLNRCAHVPTELDWQPGEFLDADREYIVCGTHGAVYDPRTGLCLAGPCARARL
                     TPIAADEREGVVYWYPSRDIRPPAAPS"
     gene            complement(2535..3215)
                     /locus_tag="VFV25_06900"
     CDS             complement(2535..3215)
                     /locus_tag="VFV25_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEY1393086.1"
                     /translation="MSEFRPRRYDLLVFDWDGTLFDSTALIVKCIQAACRDLEVAAPS
                     DEQAAYVIGLGLHDALQQAVPGLAVERYPELGQRYRHHYFARQHELSLFPGTLAMLRG
                     LKARNHWLAVATGKSRHGLEEALKTVVGGVPVRDFFDATRTADETASKPDPQMLHELM
                     REFGVEPERTLMIGDTVHDLQLAANAGTPAIAVGYGAHDDATLSGFAPRFIASSVPEL
                     HDWLLNHA"
     gene            complement(3224..>3967)
                     /locus_tag="VFV25_06905"
     CDS             complement(3224..>3967)
                     /locus_tag="VFV25_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEY1393087.1"
                     /translation="ERTATAPAPAREFPVVYEDDWLLAVDKPAGVAVHGGSGVSFGVI
                     EQLRQARPGAKFLELVHRLDKETSGLLLVAKKRSALTALQDQFRSRDTGKTYAALVIG
                     AWPAKLKVIDVALHKTLDAAGERHVRAVQADHADGKRSITLVKVARAFEAYTLLDVTL
                     KTGRTHQIRVHLAHAGHAIVGDPKYGDFTLNKQLARGEGLNGLRFDRMFLHARRLRFD
                     HPASGEVIELEAPLPDECLTLLTRLSEPH"
BASE COUNT          660 a         1262 c         1349 g          696 t
ORIGIN      
        1 agtccagttc cgtaagcggg cccagctttg gattcagtcg acgatccttg gcacgcgcgc
       61 gatcgaccac gcggcggccc cacgcatcgg gagcagtgtc tgcaatcgcc aagtggaaca
      121 ctgagtcgtg agccgtcggt gccttgcgat tctgaaaacc agcaacgagc ggcaggtcag
      181 gcgagacctc aaagcggttt gcgtccgcaa gccaggccgt gacgtactgg aactgcgaaa
      241 actcacgcct gccttgctgg acgaaaacca gctcacccac ctcggtgcct ttggttccaa
      301 ggtgaacttg cagtgcggtg cgctggttcg cgggggtcgg tcgaattggc atggtccggc
      361 tcctcagaag gcgttttggc ctttgcgggc gcgcacccgc tgcggcaggc cttcgtcggc
      421 tagagccagc ccgatgtcgt cggtcgctga gtcgagaagc gctttcagct tgtccagttc
      481 cccgaagacc agcagcacgc gtgcaagcac gtgaacctgc attcgagagt cgccggcttc
      541 caagcgcctg atcgtgctga ggctgacgcc tgcccgcagc gcgagatccg cttgggtcat
      601 tgcccgccgc cgtcgcgcgc gactgaagtc gagcgccagc tgagtaattg cgcgttgtgc
      661 aggctgtggc agtggcctag ccggagtaga gccctcatca gagcgtttac ggttcttagc
      721 ggttcccatg agatcgatta tcctgccaca gagggggacg gccattcaag catttaatca
      781 ctctattcgg agcgtttatt aataacaagc gttcctatga gaggtgttat gcccaaatcc
      841 agaggcgccc gcttgccggc gcaaacgctt catcgccagt aatcaactcg atctcgcgat
      901 actgcccccg tgtgaggtcg ccccagacct cgagcagata caggcgcaga agaaatcttt
      961 gccaaagaaa atgccctctg aaccaagcag gtcagagggc agtctggtct tctggcgatc
     1021 ggcatcaaca cttttaccga acgcctaggc cagaggcgcc ccgttttgga tgccctcgat
     1081 gcgtgctcag tgcaggccgc ccatcgcctg catggcatgc acggcgcctg cgccgatgga
     1141 agcgccgaag cgcttggcca gccgctcggc gacgttctcc ttggtcgtgt agtcgaccac
     1201 gtcttcggcc ttgacgactt cacgcgcgat gtagtcgagg ctgcccagat ggtcggccag
     1261 accctggtct atggcctgct ggccgttcca gaacaggcca gagaaggtct cgggcgtttg
     1321 cttcaggcgc ttgccgcgcc cctgcttgac caccgagatg aactgctggt ggatctggtc
     1381 gatcatggcc tgggcatagg cgcgctgctt gtcgttctgc ggcgagaagg gatcgagcat
     1441 gcccttgttg tcgccggccg tgagcaggcg gcgttcgatg ccgagcttgt ccatcaggcc
     1501 gacgaagccg aagccgtcca tcagcacgcc gatggagccg accagcgagg ccttgtcgac
     1561 atagacctcg tcggcgccga cggcgatgta gtaagccgcc gaggcgcaca tctcctcgca
     1621 tacggcatag agcttcctgt ccttgtgcag ggctctcagg cggcggatct cgtcgttgat
     1681 caggccggcc tgcaccgggc tgccgccggg cgaattgatg cgcagcacga cccccttggc
     1741 atcgctggca tcgaaggcgt tgcgcagcgc cgtgatgacg ttctcggcgc tggattcggc
     1801 atcggccgcg atctcgccgt gcacctcgat cagtgccgtg tgcgggccgc tgggcgcatt
     1861 ggccaggtta cgctcgttga cggcggcgaa gacgatggct gcgaacagca gcagccaggc
     1921 gaggcggaag aagatgcgcc agcgccgctc gctgcgcctg tcggccagat agctgcgggc
     1981 gaattcctcg accacgcccc aggaagtggc aggcaggtcg ggcgcggcgg cgggtgccgg
     2041 cggggggggt gcgccgaacg tcggctcagg gcgcgaagga tcgtcgtgat tgctcatggc
     2101 tgctgatctc tggtgaggag gaggccgctt gggcggcggc tgccgctcag gcttaggagg
     2161 gcgcggcggg cggccggatg tcccgggaag gataccaata caccacgcct tcgcgctcgt
     2221 cggcggcgat cggcgtcagg cgggcgcgcg cgcaaggacc ggccaggcag agcccggtgc
     2281 gcggatcgta gaccgcccca tgcgtgccgc agacgatgta ctcccggtct gcgtcgagaa
     2341 actcgccggg ctgccaatcc agctcggtgg gcacatgggc acagcggttc agataggcca
     2401 ccaccctgcc ctcgaagcgc agcgcgaagg cgcgcacgct gcgcccgtat tcgagcacgt
     2461 cgaagacctg cgccggcccg cgctcggcaa ggtcggccga ggtgcaaagc gccacgcctt
     2521 gggaagcctg cgtctcaagc atggttcagc agccaatcgt gcagctcagg cacggagctg
     2581 gcgatgaagc ggggcgcgaa tccgctcagg gtcgcatcgt cgtgggcgcc atagcccacg
     2641 gcaatggcgg gcgtgccggc attggccgcg agttgcaggt cgtgaacggt gtcgccgatc
     2701 atcagcgtgc gctcgggctc gacgccgaac tcgcgcatca gctcgtgcag catctgcggg
     2761 tcgggcttgg aagcggtttc gtccgcagtg cgcgtggcat cgaagaaatc gcgcaccggc
     2821 acgccgccga cgacggtctt caaggcttcc tccaagccgt ggcgcgactt gccggtggcc
     2881 accgcgagcc agtgatttcg ggccttgagc cctcgcagca tcgccagcgt gccgggaaac
     2941 aggctcagct catgctggcg cgcgaaatag tggtggcgat agcgctggcc gagttcaggg
     3001 tagcgctcca ccgccaggcc gggcacggcc tgctgcagcg catcgtgcag gcccaggccg
     3061 atcacgtagg ccgcctgctc atcgctgggc gcagccactt cgaggtcgcg gcaggcggcc
     3121 tgtatgcact tgacgatgag cgccgtcgaa tcgaacaggg tgccgtccca gtcgaagacg
     3181 agcaggtcgt agcggcgcgg gcggaattcg gacatcggtc ggatcaatgc ggttcggaca
     3241 accgggtcag cagtgtgagg cattcgtcgg gcaagggcgc ttccagctcg atgacctcgc
     3301 cgcttgcggg gtggtcgaag cgcaggcggc gcgcgtgcag gaacattcgg tcgaaccgca
     3361 ggccgttcag gccctcgccg cgcgccagct gcttgttgag cgtgaagtcg ccgtacttgg
     3421 gatcgccgac gatggcgtgc ccggcatgtg ccaggtgcac gcggatctga tgcgtccgcc
     3481 cggtcttgag ggtcacgtcc agcagcgtgt aggcctcgaa ggcccgcgcc accttgacga
     3541 gcgtgatcga gcgcttgccg tcggcatggt cggcctgcac cgcgcgcaca tggcgctcgc
     3601 cggcggcatc cagggtcttg tgcagggcca cgtcgatgac cttgagcttg gccggccagg
     3661 ctccgatcac cagcgcggcg taggtcttgc ccgtgtctct gctgcgaaac tggtcttgca
     3721 acgcggtcag cgcgctgcgc ttcttggcca ccagcagcag ccccgaggtt tccttgtcca
     3781 gccggtgcac gagctccagg aatttcgcgc cggggcgcgc ctggcgaagc tgctcgatga
     3841 cgccgaagct cacgccgctg ccgccatgca cggccacgcc tgcgggcttg tccacggcga
     3901 gcagccagtc gtcctcatag accacgggga attcgcgcgc gggagcgggg gctgtcgccg
     3961 tcctctc
//
LOCUS       DASUCY010000351         3111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1099934, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000351 DASUCY010000000
VERSION     DASUCY010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1099934"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VFV25_06910"
     CDS             <1..252
                     /locus_tag="VFV25_06910"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY1393088.1"
                     /translation="LGRALLAELLARCEALGARQMLAVIGDSNNLGSIRLHRALGFND
                     LGVMKAVGWKFGRWLDVVLMQKGLGLSNSAAPIEYGTAS"
     gene            249..692
                     /locus_tag="VFV25_06915"
     CDS             249..692
                     /locus_tag="VFV25_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HEY1393089.1"
                     /translation="MSTYKSKTLAAWLALLGGSFGLHRFYLYGLRDRLGWLHPWPTLL
                     GLYGVQRVLEFGQDDQLAWALIPLLGMMLAATMLMAIIYGLMPDEEWNARYNPQGPEH
                     HASWSTVFAVVLALALGATVLMSTIAFGVQRYFEYQIDEARKISQ"
     gene            complement(709..1719)
                     /locus_tag="VFV25_06920"
     CDS             complement(709..1719)
                     /locus_tag="VFV25_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEY1393090.1"
                     /translation="MEAVQLSFWVVALLVVLAVVFDFMNGFHDAANSIATVVSTGVLR
                     PQQAVVFAAAFNFVALFIFHLSVAATIGKGIVLPGVVDQHVVFGALVGAIAWNLITWW
                     FGIPSSSSHALIGGIVGAVIAKTGAGSLIAAGIWKTVLFIFLSPLLGFVLGSALMALI
                     AWLFRGKTPLRIDRWFRRAQLVSAGLYSLGHGGNDAQKTIGIIWMLLIASGYASASAA
                     MPPVWTIWCCYLAIGLGTMFGGWRIVKTMGQRITKLKPVDGFCAETGGAITLFIATAL
                     GVPVSTTHTITGAIVGVGSTRRASAVRWGVAGNIVWAWIFTIPAAAFIAAVFYLVSLW
                     LF"
     gene            complement(1735..2382)
                     /locus_tag="VFV25_06925"
     CDS             complement(1735..2382)
                     /locus_tag="VFV25_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HEY1393091.1"
                     /translation="MLFGKLLPREGNFFELFNQHSDQIVAGSRAFLALIQGYDDPMLR
                     EQHGASVDNAERQADKITAEVNRLLHKTFITPIDREQIHGLINAMDDVLDLLQDATET
                     MALYDVRTITDETRRLGELSAKCCERVAYAVSLLPKLSEHAAAEAALKTCEEIDKLES
                     DADRVMRAAMSKLFREEQDVRELIKLKAIYEQLESITDRCEDVANLIEGIVLENS"
     gene            complement(2450..>3111)
                     /locus_tag="VFV25_06930"
     CDS             complement(2450..>3111)
                     /locus_tag="VFV25_06930"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEY1393092.1"
                     /translation="IGVGKSSLAGKLVQAFGARLFLERPEENPYLERYYAAGTEDLAA
                     NPYALQTQLFFLFQRLEQMRELAQGGMFERGLVSDFLFAKDALFARLTLGDEDHALYS
                     GIYRRYEPQVPAPDLVIWLRADTPTLKVRIAQRGLAMEQDIAETYLQRLSDAYASHFE
                     ADAQTPLLAIDTEAFNPLDSQADFALLLQRLAAFAGPRERLEAGAGEAGQLPPGATLP
                     L"
BASE COUNT          525 a         1028 c         1046 g          512 t
ORIGIN      
        1 ctgggccgag cgctgctggc cgagttgctg gcccgctgcg aagccctggg cgcgcgacag
       61 atgctggccg tcatcggcga ctcgaacaac ctgggctcca tccgcctgca ccgcgccctg
      121 ggcttcaacg acctgggcgt catgaaggcc gtgggctgga agttcggccg ctggctcgat
      181 gtggtgctga tgcagaaagg tctgggcctg agcaacagtg ctgctcccat cgaatacggc
      241 accgcgtcat gagcacatac aaatcaaaga ccctcgccgc ctggctcgcc ttgctgggcg
      301 gcagcttcgg cctgcatcgc ttctatctct acggcctgcg cgaccgcctg ggctggttgc
      361 acccctggcc tacgctgctc ggcctgtatg gcgtgcaacg tgtgctggag ttcggccagg
      421 acgaccagct ggcctgggcg ctgatcccgc tgctgggcat gatgctggcc gccaccatgc
      481 tgatggccat catctatggc ctgatgcccg acgaggaatg gaacgcccgc tacaacccgc
      541 agggccccga acaccacgcc agttggtcga ccgtattcgc cgtcgtgctg gcgctggccc
      601 tgggcgccac ggtgctgatg tccaccatcg ccttcggcgt gcagcgctac ttcgagtacc
      661 agatcgacga ggcgcgcaag ataagccagt gagccggcgt cgccggcttc agaacagcca
      721 gaggctgacc aggtagaaca ccgcggcgat gaaggctgcg gcggggatcg tgaagatcca
      781 ggcccagacg atgttgccgg ccacgcccca gcgcaccgcc gaggctcggc gcgtggagcc
      841 gacgccgacg atcgcgccgg tgatggtgtg cgtggtcgac accggcacgc ccagcgcggt
      901 cgcgatgaag agcgtgatgg cgccgcccgt ctcggcgcag aaaccgtcca ccggcttgag
      961 cttggtgatc ctctggccca tggtcttgac gatgcgccag ccgccgaaca tcgtgcccag
     1021 gccgatcgcg agatagcagc accagatcgt ccacaccggc ggcatcgccg cactcgcgct
     1081 cgcatagcct gaggcgatca gcagcatcca gatgatgcct atggtcttct gcgcgtcatt
     1141 gccgccgtgc cccaggctgt agagcccggc cgagacgagc tgggcgcggc ggaaccagcg
     1201 gtcgatccgc agcggcgtct tgcccctgaa cagccaggcg atgagcgcca tcagcgccga
     1261 gccgagcacg aagcccagca gcggcgacag gaagatgaag agcacagtct tccagatgcc
     1321 ggcggcgatc agagagccgg cgccggtctt ggcgatgacg gcgccgacga tgccgccgat
     1381 cagcgcatgc gaggagctgg acgggatgcc gaaccaccag gtgatcaggt tccaggcgat
     1441 ggcgccgacc agggcgccga acaccacgtg ctggtcgacc acgcccggca gcacgatgcc
     1501 cttgcctatc gtcgccgcca cgctcaggtg gaagatgaag agggcgacga agttgaaggc
     1561 cgcggcgaac accaccgcct gctgaggcct gagcacgcct gtggatacga cggtggcgat
     1621 cgagttcgcc gcgtcgtgaa agccgttcat gaagtcgaac acgaccgcca ggacgaccag
     1681 cagggcaacc acccagaaac tgagctgaac cgcttccatc tgcgcggccg ctgctcagga
     1741 gttctcgagg acgatgccct cgatcagatt ggcaacgtcc tcgcagcggt cggtgatgga
     1801 ttcgagctgc tcgtagatgg ccttgagctt gatcagctcg cgcacatcct gctcctcgcg
     1861 gaagagcttg ctcatcgcgg cgcgcatcac gcggtcggcg tcggattcga gcttgtcgat
     1921 ctcctcgcag gtcttgagcg ccgcctccgc cgcggcgtgc tcgctcagct tgggcagcag
     1981 gctcaccgca taggccacgc gctcgcagca cttggcggag agctcgccca ggcggcgcgt
     2041 ctcgtcggtg atggtgcgca cgtcgtacag cgccatggtc tcggtggcgt cctgcaagag
     2101 atccagcacg tcgtccatcg cattgatcag gccgtggatc tgctcgcggt cgatcggcgt
     2161 gatgaaggtc ttgtgtagca ggcggttcac ctcggccgtg atcttgtcgg cctggcgctc
     2221 ggcgttgtcc acgctcgccc cgtgctgctc gcgcagcatg ggatcgtcat agccctggat
     2281 cagcgccagg aaggcacgcg agccggcgac gatctggtcg ctgtgctggt tgaacagttc
     2341 gaagaagttg ccctcgcggg gcagcagctt gccgaacagc atttgggagc ctccaacgca
     2401 agacgacccg gccgcgccgt gcagccgggt cgcatttttc atggttttgt cacaacggaa
     2461 gtgtagcgcc cggcggcagt tgtccagcct cgcccgcgcc ggcttctagc cgctcgcgcg
     2521 ggccggcaaa ggccgccagg cgctgcagca gcagcgcaaa gtcggcttgg ctgtccagcg
     2581 ggttgaaggc ttcggtgtcg atggccagga gcggcgtctg ggcatcggcc tcgaagtggc
     2641 tcgcgtaggc gtcgctcagc cgctgcagat aggtctcggc gatgtcctgc tccatcgcca
     2701 ggccgcgctg cgcgatgcgc accttgagcg tgggcgtgtc tgcgcgcagc cagatcacga
     2761 ggtccggcgc cggcacctga ggctcgtagc ggcgatagat cccgctgtag agcgcgtggt
     2821 cctcgtcacc cagcgtcagc cgcgcgaaca aggcgtcctt ggcgaacagg aagtcgctca
     2881 ccagcccgcg ctcgaacatg ccgccctgag ccagctcgcg catctgctcc aggcgctgga
     2941 acaggaagaa gagctgggtc tgcagcgcat agggattggc cgcgaggtcc tcggtgccgg
     3001 ccgcgtaata gcgctcgaga taggggttct cctcgggccg ctcgaggaag agccgtgcgc
     3061 cgaaggcctg caccagcttg cccgccagcg agctcttgcc cacgccgatg g
//
LOCUS       DASUCY010000352         3586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_280738, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000352 DASUCY010000000
VERSION     DASUCY010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280738"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VFV25_06935"
     CDS             <1..221
                     /locus_tag="VFV25_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HEY1393093.1"
                     /translation="ERRLAADAATGRFCHGDAPTLADCVLVPQIFNAQRFDCRLDHLP
                     TLMRIFEACMALDAFQQAQPSASPDATA"
     gene            287..997
                     /gene="pgeF"
                     /locus_tag="VFV25_06940"
     CDS             287..997
                     /gene="pgeF"
                     /locus_tag="VFV25_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEY1393094.1"
                     /translation="MSTRAGGVSRAPYDSMNLGTFVGDAEDAVQANQRRLAERMGGAQ
                     LVFLRQVHGTRVLNLDELQALPQQPIEADASITTRPGHACTVTVADCLPVLFAAPEAR
                     GVGAAHAGWRGLAGGVLENTVRALCEAADCEPDQLVAWLGPCIGPRQFEVGADVVQAF
                     GSAAGLRFRPRPGLAGKWLADLPALARDRLSALGLSRISGGHWCTVEDASRFFSFRRD
                     RVTGRMAACVWLDASRSR"
     gene            complement(914..1192)
                     /locus_tag="VFV25_06945"
     CDS             complement(914..1192)
                     /locus_tag="VFV25_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393095.1"
                     /translation="MYIVAIAWLYVVLMMAAAEATSPQGTLLGAIFTFLLYGLGPLAL
                     VLYLLATPARRRARRAREAQASATPGGVEPDAGGHAPGDAVAPERKET"
     gene            1348..3048
                     /gene="phaC"
                     /locus_tag="VFV25_06950"
     CDS             1348..3048
                     /gene="phaC"
                     /locus_tag="VFV25_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HEY1393096.1"
                     /translation="MASRTRRGPAAKAATKTPPSQDVLPPGAEAFGQSVGGALQSLAG
                     LSLPFNAAAQLQSDYLQQAAALWNQALGQAEAKPNGDRRFAAEDWTGSPVSSFTAGMY
                     MLNARTLLQMAESLEGDEKTRQRVRFAVQQWNDAASPANYLALNPEAQRRALETGGES
                     ISRGLQHLWRDVQQGHVSQTDESVFEVGRNVATTAGSVVYENELFQLIEYQPLTGQVH
                     ERPLLIVPPCINKFYILDLQPDNSLVRYAVSQGHRVFLVSWANADESLAHKTWDDYIQ
                     DGVMRAIAEVRAISRQPQINTLGFCIGGTILATALAVLAAKGEKPAASVTLLTTFLDF
                     SDTGVLALFVDEAAVQMREMAIGEGGLLKGQELASTFSFLRPNDLVWNYVVGNYLKGE
                     TPPPFDLLYWNSDGTNLPGPMFCWYLRNTYHENKIKEAGKLSVCGVPLDLGLVDTPAF
                     IYASREDHIVPWDSAYASTALLPGKKQFVLGASGHIAGVINPPEKKKRSFWTGARLPT
                     KASDWLNSAQESPGSWWPTWAAWLATHAGKQVPAPKAPGDRQHPVIEPAPGRYVKQKA
                     "
     gene            3111..>3586
                     /locus_tag="VFV25_06955"
     CDS             3111..>3586
                     /locus_tag="VFV25_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEY1393097.1"
                     /translation="MSTDIVIVAATRTAVGKFGGTLSKTPASELGAIVIKELLKRAKL
                     SGEQINEVILGQVLTAGVGQNPARQAVIKAGLPQGVPAMTINKVCGSGLKAVMLAAQA
                     IRDGDSEIIIAGGQESMSMAPHVLNGSRDGQRMGDWKMIDSMIVDGLWDVYNQYHMG"
BASE COUNT          618 a         1210 c         1208 g          550 t
ORIGIN      
        1 tggagcgccg cctggcggcc gatgcggcga ccgggcgctt ctgccatggc gatgcgccga
       61 cgctggccga ttgcgtgctg gtgccgcaga tcttcaacgc ccagcgcttc gactgccgcc
      121 tcgaccactt gcccacgctg atgcgcatct tcgaggcctg catggcgctg gatgccttcc
      181 agcaagcgca gccctcggcc agcccggacg ccacagcgtg aatcccgcaa gcgatcccac
      241 ctggctgctg cccgactggc ccgcgcccag cggcgtcggc gccttgatga gtacacgggc
      301 cggcggcgtc agccgggcgc cctatgactc catgaacctc ggcacctttg tgggcgacgc
      361 cgaagacgcg gtgcaggcca accagcggcg cctcgccgaa cgcatgggcg gcgcgcagct
      421 ggtcttcctg cgccaggtgc atggcacgcg tgtgctcaat ctcgatgagc tgcaggccct
      481 gccgcagcag cccatcgagg cggatgccag catcacgacg cggccgggcc atgcctgcac
      541 ggtcacggtg gccgattgcc tgccggtgct gttcgcggcg cccgaggcgc gcggcgtcgg
      601 cgcggcccat gccggctggc gcggcctggc cggcggcgtg ctggagaaca cggtgcgcgc
      661 gctgtgcgaa gcggccgatt gcgaaccgga tcagctcgtg gcttggctcg ggccctgcat
      721 cgggccgagg cagttcgagg tgggtgccga cgtcgtgcag gccttcggca gcgccgctgg
      781 gctgcgcttc aggccgcgcc ctggtctggc gggcaaatgg ctagccgatc tgccggcgct
      841 ggcccgcgat cgcttgtcgg cgctgggcct gagccgcatc agcggcgggc actggtgcac
      901 cgtcgaagac gcctcacgtt tcttttcgtt ccggcgcgac cgcgtcaccg ggcgcatggc
      961 cgcctgcgtc tggctcgacg cctcccggag tcgctgaggc ctgcgcctcg cgggcccggc
     1021 gtgccctgcg ccgcgccggc gtggcgagca ggtacagcac cagggccagc ggccccagcc
     1081 catagagcag aaacgtgaag atcgcgccca gcagcgtgcc ctgcggagac gtcgcttcgg
     1141 ccgccgccat catcaacacg acgtagagcc aagcgatggc gacgatgtac atggcgggca
     1201 tcaagcctac gatagttcag gatgtcattc tgtattaagg gacccgtgcc cataatgcga
     1261 taaaacatgc ggggccagtc gggatcagcg caagcacgag cttcgcgggc atcatggcag
     1321 cataacgagc gagataacga ggagaccatg gccagcagaa ctcgacgcgg cccagcggcc
     1381 aaggcggcga ccaagacgcc gccgtcccag gatgtcctgc cgccaggcgc cgaggccttc
     1441 gggcaatcgg tgggcggcgc gctccaatcc ctggccggcc tgagcctgcc cttcaatgcg
     1501 gcggcgcaac tgcaatcgga ctatctgcag caggccgccg cactatggaa ccaggcgctg
     1561 ggccaggccg aggccaagcc caacggcgac cggcgctttg ccgccgaaga ctggaccggc
     1621 agcccggtct cgtccttcac ggccggcatg tacatgctga atgcccgcac gctgctgcag
     1681 atggccgaga gcctagaagg cgacgagaag acgcgccagc gcgtgcgctt tgccgtgcag
     1741 cagtggaacg atgcggcctc gcccgccaac tacctcgcgc tcaatcccga agcgcagcgc
     1801 cgtgcgctgg aaaccggcgg cgaaagcatc tcgcgcggcc tgcagcacct gtggcgcgac
     1861 gtgcagcagg gccatgtgtc gcagaccgat gagagcgtgt tcgaggtcgg ccgcaatgtc
     1921 gcgacgacgg caggctcggt ggtctacgag aacgagctgt tccagctcat cgagtaccag
     1981 cctttgacgg gccaggtgca cgagcggccg ctgctcatcg tgccgccttg catcaacaag
     2041 ttctacatcc tcgacctgca gccggacaac tcgctggtgc gctatgccgt gtcgcagggg
     2101 catcgcgtct tcctggtgag ctgggccaat gccgatgaga gcctggccca caagacctgg
     2161 gacgactaca tccaggacgg cgtgatgcgc gccatcgccg aagtgcgcgc catcagccgg
     2221 cagccgcaga tcaacaccct gggcttctgc atcggcggca ccatcctcgc cacggccctg
     2281 gcagtgctgg cggccaaggg cgagaagccg gcggccagcg tgacgctgct gaccaccttc
     2341 ctcgacttct ccgacaccgg cgtgctcgcc ctcttcgtcg acgaggctgc ggtgcagatg
     2401 cgtgagatgg ccataggcga gggcggcctg ctcaaggggc aagagttggc gtcgaccttc
     2461 tccttcttgc gccccaacga cttggtctgg aactacgtgg tgggcaacta cctcaagggc
     2521 gaaacgccgc cgcccttcga cctgctgtac tggaattccg acggcaccaa cctgccgggg
     2581 ccaatgttct gctggtattt gcgcaatacc taccacgaga acaagatcaa ggaagccggc
     2641 aagctcagcg tctgcggcgt gccgctggac ttagggctgg tggacacgcc ggccttcatc
     2701 tatgcctcgc gcgaggatca catcgtgccc tgggacagcg cctatgcctc cacggccctg
     2761 ttgccgggca agaagcaatt cgtgctgggc gcctcgggcc atattgccgg cgtgatcaat
     2821 ccgcccgaga agaagaagcg cagtttctgg accggtgccc gcctgcccac caaggcgagc
     2881 gactggttga attcggccca ggaaagccct ggcagttggt ggccgacatg ggcagcctgg
     2941 ctggctaccc acgccggcaa gcaggtcccc gcgccaaaag cgccgggcga caggcagcac
     3001 ccggtgatcg aaccggcgcc cggccgctat gtgaagcaga aagcttgagc gcatgatgat
     3061 gcgcagcagt cccctggtca gtgtcattga gtcgtagaag gagtccacgc atgagtaccg
     3121 atatcgtgat cgttgccgcc acccgcaccg ctgtgggcaa gtttggcggc acgctgtcca
     3181 agacgccggc gtccgagctg ggcgccatcg tcatcaagga gctgctcaag cgcgccaagc
     3241 tgagcggcga gcagatcaac gaggtcatcc tgggtcaggt gctgaccgcc ggcgtgggcc
     3301 agaacccggc gcgccaggcc gtcatcaagg ccggcctgcc tcagggcgtg ccggccatga
     3361 ccatcaacaa ggtctgcggc tcgggcctga aggccgtgat gctggcggcg caggcgatac
     3421 gcgacggcga ttcggagatc atcatcgccg gcggccagga gagcatgagc atggcgcccc
     3481 acgtgctcaa cggctcgcgc gacggccagc gcatgggcga ctggaagatg atcgactcga
     3541 tgatcgtcga cggcctgtgg gacgtctaca accagtacca catggg
//
LOCUS       DASUCY010000353         3233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_361028, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000353 DASUCY010000000
VERSION     DASUCY010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361028"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VFV25_06960"
     CDS             <1..399
                     /locus_tag="VFV25_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007744023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HEY1393098.1"
                     /translation="NRRSGYYDLGLCGPMRQDAIAKQRQYCGYFKTPTLRNVATRSVF
                     FHNGVFRSLEDVVRFYVERETRPEHWYARSSDGKLLRYDDLLRAAHANVDLIDPPFDR
                     KRGDAPALNEDEIRDVAAFLKTLTDGYKPQ"
     gene            complement(408..2066)
                     /locus_tag="VFV25_06965"
     CDS             complement(408..2066)
                     /locus_tag="VFV25_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEY1393099.1"
                     /translation="MSEASTFDYIIVGGGTAGCLLAKRLSADASKRVLLVEAGGRDDY
                     HWIHIPVGYLYCIGNPRTDWLYFTEPDPGLNGRSLRYPRGKVLGGCSSINGMIYMRGQ
                     SRDYDGWAQLVGDEAWRWDACLPYFKRHEDYHAGADAMHGAGGEWRVERQRLRWDILD
                     AFAQAAQQAGIPASADFNRGNNEGVGYFEVNQRDGWRWNTAKAFLRPACYGRPNFELW
                     TGAQVRRLLLERRTDGSLSCSGAEVATPQGIATVRAARELILCAGSIGTPQLLQLSGI
                     GPGELLQRHGIGVQHELPGVGENLQDHLQVRAVYKVKGAATLNTRAATLWGKAGIALE
                     YALKRSGPMSMAPSQLGAFTRSSPQQAWPNLEYHVQPLSLEAFGEPLHDFDAFTASVC
                     NLNPTSRGSVRIKSAKPEDAPAIAPNYLSTPEDRQVAADSLRLTRRIAAQPALAQYEP
                     EEFKPGPQYQSDEDLARLAGDIGTTIFHPVGTCKMGRDDDAAAVVDARLRVYGPGGPG
                     GVIAGLRVVDASVMPTITSGNTNSPTLMIAERAAQWIVEAARAG"
     gene            2192..2467
                     /locus_tag="VFV25_06970"
     CDS             2192..2467
                     /locus_tag="VFV25_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY1393100.1"
                     /translation="MTEVYRDWINTLKDRTARARIQVRVDRLVHGNPGQHRHLTESVS
                     ELKIDAGPGYRVYYTERNGEVIILLAGGDKSSQQQDIKLAITLARNL"
     gene            2477..2794
                     /locus_tag="VFV25_06975"
     CDS             2477..2794
                     /locus_tag="VFV25_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antidote protein"
                     /protein_id="HEY1393101.1"
                     /translation="MTKASAKTSAKTKTAPYDIAEQLRTPEEMAAYLDAWLIEAPDDA
                     AGIARALGDIARAKGMSQVARDAGLSRESLYKALSENGNPSFATVLKVARALGVRLHA
                     EPA"
     gene            complement(2805..3086)
                     /locus_tag="VFV25_06980"
     CDS             complement(2805..3086)
                     /locus_tag="VFV25_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEY1393102.1"
                     /translation="MKAIWNDTVVAESDDTVVVEGNHYFPEGAVKREYLLPSNTKTMC
                     SWKGQASYYNLFVDGDVNPDAVWYYPEPYEAAAQIKGRVAFWKGVKVQE"
BASE COUNT          553 a         1106 c         1034 g          540 t
ORIGIN      
        1 aaccgccgca gcggctacta cgacctgggc ctgtgcgggc cgatgcggca agacgcgatc
       61 gcgaagcagc gccaatactg cggctacttc aagacaccga cgctgcgcaa cgtggccacg
      121 cgcagcgtct tcttccacaa cggcgtcttc cgctcgctcg aagacgtggt gcgcttctac
      181 gtcgagcgcg agacccggcc cgaacactgg tacgcgcgct cgagcgacgg caagctgctg
      241 cgctatgacg acctgctgcg cgcagcgcat gccaatgtcg acctgatcga tccgcccttc
      301 gaccgcaagc gcggcgatgc gccggcactg aacgaagacg agatccgcga cgtcgccgcc
      361 ttcctgaaga cgctgaccga cggctacaag ccgcaatagc ggggacgtca gccggcgcgt
      421 gccgcctcga cgatccactg cgccgcgcgc tcggcgatca tcagcgtcgg cgaattggtg
      481 ttgccgctgg tgatcgtggg catcacgctc gcatcgacaa cgcgcagacc ggcgatcacg
      541 ccgcccgggc ctcccggacc gtacacgcgc aggcgcgcgt ccaccaccgc ggcggcgtcg
      601 tcgtcacgcc ccatcttgca ggtgcccacg gggtggaaga tggtcgtgcc gatatcgccg
      661 gccaggcggg ccagatcctc gtcgctctgg tactggggcc cgggcttgaa ctcctcgggc
      721 tcgtactgcg ccagcgccgg ctgggccgcg atgcggcgcg tcagccgcag cgaatcggcc
      781 gccacctggc ggtcttcggg cgtggacaga tagttgggtg cgatcgccgg cgcgtcctcg
      841 ggcttggccg acttgatgcg caccgagccg cggctggtcg ggttgagatt gcacacgctg
      901 gcagtgaagg catcgaagtc gtgcagcggc tcgccgaagg cctcgagcga cagcggttgt
      961 acgtggtact ccagattggg ccaggcctgc tgcggcgacg agcgcgtgaa ggcgcccagt
     1021 tgcgaaggcg ccatgctcat cgggccgcta cgcttgagcg cgtattcgag cgcgataccg
     1081 gccttgcccc acagcgtcgc ggcgcgcgtg ttgagcgtgg ccgcgccctt gaccttgtag
     1141 acggcgcgga cctgcagatg gtcctgcagg ttctcgccga cgccgggcag ctcatgctgc
     1201 actccaatgc catgtcgctg caacagctcc ccggggccta tgcccgagag ctgcaggagc
     1261 tgcggcgtgc ctatgctgcc cgcgcacagg atcagctcgc gcgccgcacg caccgtcgct
     1321 atgccctgcg gcgtcgccac ctcggcgccg ctgcaactca aggagccgtc ggttcgacgc
     1381 tccagcagca gacgccgcac ctgggcgccg gtccacagct cgaagttggg ccggccataa
     1441 caggctgggc gcaggaaggc cttggccgtg ttccagcgcc agccgtcgcg ctggttgacc
     1501 tcgaaatagc ccacgccctc gttgttgccg cgattgaagt ccgcgctggc cgggatgccg
     1561 gcctgctgcg ccgcctgcgc gaaggcatcg aggatgtccc agcgcaggcg ctggcgctcc
     1621 acgcgccact cgccgccggc gccatgcatt gcatcggccc cggcgtgata gtcctcgtgg
     1681 cgcttgaagt aaggcaggca ggcgtcccaa cgccaggcct catcgccgac gagctgggcc
     1741 caaccgtcgt agtcacgcga ctggccgcgc atgtagatca tgccgttgat gctggagcag
     1801 ccgcccagca ccttgccgcg cggatagcgc aggctgcgcc cgttcaagcc agggtccggc
     1861 tcggtgaaat acagccaatc cgtgcgcgga ttgccgatgc aatacaggta gccgaccggg
     1921 atgtggatcc agtgatagtc gtcgcgtccg cccgcctcga ccagcagcac gcgcttcgag
     1981 gcatcggcgc tcaggcgttt ggcaagcagg cagccggcgg tgccgccacc gacgatgatg
     2041 tagtcgaagg ttgatgcctc gctcacgcca tttcgctttc cgaccttgtc atgacccgcg
     2101 gagtatgccg agctgcagcc acgatggctt cagcagcacg cccttgacgc ccgtatccca
     2161 caggatacac ttccctgcta tgcaggtgcg gatgacggag gtctatcgag actggatcaa
     2221 caccctcaag gaccgcacgg cacgcgcgcg cattcaggtg agagttgatc gcctggttca
     2281 cggaaatccg ggacagcatc gccatctcac cgagagcgtt tctgaattga agatcgatgc
     2341 cggcccgggc taccgcgtct actacaccga acggaacggc gaagtgatca ttctgctcgc
     2401 gggcggcgac aagtcgagcc aacagcagga catcaagctg gccatcaccc tggcgagaaa
     2461 tttgtaggag tgtttgatga caaaagcctc tgccaagacc tctgcaaaaa ccaaaacggc
     2521 cccctatgac atcgctgagc agctgcgcac tcccgaagaa atggctgctt atctggatgc
     2581 ctggctgatc gaagccccag atgatgcggc gggcatcgcc cgcgcgcttg gagacattgc
     2641 gcgcgccaag ggcatgtctc aagtcgcgcg tgacgccggc ctcagccgcg aaagcctgta
     2701 caaggcgctc agcgagaacg gcaaccccag cttcgccact gtcctcaaag tcgcgcgcgc
     2761 tctcggtgtg cggctacatg cagagccggc ctaaggagcc tcgctcactc ctgcaccttc
     2821 acacccttcc aaaacgccac ccggcccttg atctgcgccg cggcttcgta gggctcgggg
     2881 tagtaccaga cggcgtcggg gttcacgtcg ccgtcgacga agaggttgta gtagctcgcc
     2941 tggcccttcc acgagcacat ggtcttggtg ttgctgggca gcaggtactc gcgcttgacc
     3001 gcgccttcgg ggaaatagtg attgccttcg accaccacgg tgtcgtcgct ctcggcgacc
     3061 actgtgtcgt tccagattgc cttcatcgtc atcctttcac tcatgacagc ccctcgtccg
     3121 acgagtacgt cggtcggtcc cccgagggga cgcgggccgg cttgggagcg gcccggcgct
     3181 cggcccgctc acgccgcggc ctcgagccag tgcacgctaa acagccgctg tgc
//
LOCUS       DASUCY010000354         6111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1485345, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000354 DASUCY010000000
VERSION     DASUCY010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 6111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6111
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1485345"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VFV25_06985"
     CDS             complement(<1..478)
                     /locus_tag="VFV25_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY1393103.1"
                     /translation="MKIFIKRWLALRAVVLAAAVSAAPVARAQPTVDDFFRAPAISDA
                     ELSPSGRYLAVGVGNMTGRIQLGVIDLDSRDRPAVVVAGFSDADINHFQWLNDERLVL
                     TVADTDRASGFLRGNGLWAVNRDGKDFRQLIERAWTIKEITARKIRLLEPDHALLGV"
     gene            589..1476
                     /locus_tag="VFV25_06990"
     CDS             589..1476
                     /locus_tag="VFV25_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY1393104.1"
                     /translation="MPFNHLLLALAVVFVWGTNFVVIKLGLAELPPFLFATLRFAASA
                     FPFLFFIKRPAVPLAKLAAFGALLGAGQFGLLFYAMRADISPGLASLVIQTQVFFTIG
                     MAMLLHRERPRAEQGIALALAVAGMGVIAAHLNGSITALGLVLVTFAALSWACANLVV
                     KSAGRVDMLGFMVWSSIFAVPPLLVLSLVFEGPTAIAQGLAHADWVGWAAVAWQAVGN
                     TLFGYGAWNWLLARHAAATVTPMALLVPVFGMAASALAFSEAMPAWKLLAAALVLGGL
                     ALNVAASRIALRSLQTARG"
     gene            1476..2129
                     /locus_tag="VFV25_06995"
     CDS             1476..2129
                     /locus_tag="VFV25_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014900742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEY1393105.1"
                     /translation="MTTMTIRLHYYPSNASMAPHMLLEELGLRYELVLVDRTVGAHKS
                     PEYLKLNPNGLIPVLEDGGLVLYETAAICLHLVDSHPHAGLAPALGTPQRAHFYKWLM
                     WLTNTLQASLSVYFYPERWADGDDAIAQVRAHAETRIVGLLQQLDAELARHGGPWLLG
                     EDYSALDPYALMLCRWTRGFEKPARSLPALGPYLQRVLARPAIRRAFEQERLPLPWI"
     gene            complement(2155..2385)
                     /locus_tag="VFV25_07000"
     CDS             complement(2155..2385)
                     /locus_tag="VFV25_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003089113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDCCVxC domain-containing (seleno)protein"
                     /protein_id="HEY1393106.1"
                     /translation="MTTSSEIELRSELTCPHCGGRSLEQMPTDACVYFYECRLCGALL
                     RPRPGDCCVFCSYGSVKCPPVQMGDEGKGCCA"
     gene            complement(2399..2731)
                     /locus_tag="VFV25_07005"
     CDS             complement(2399..2731)
                     /locus_tag="VFV25_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ family domain-containing protein"
                     /protein_id="HEY1393107.1"
                     /translation="MPTLDELIAQGLSESEANGELRAAPSYGRPLDFGDGYDETPIEL
                     RMPFKVLKDAGFVPHEVTMLRELAELRRRLAAGVSAEEEPKLTRRISELQQDIALRLE
                     KLSRAGAL"
     gene            complement(2748..4142)
                     /locus_tag="VFV25_07010"
     CDS             complement(2748..4142)
                     /locus_tag="VFV25_07010"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY1393108.1"
                     /translation="MQELHDLSAVELNTLYAQGRLSPVEAARAVIAHIEAWEPKLRAT
                     YAFDPDAALVMAQASERRWHAGQPLSALDGVPAMIKENIATRGVPVPLGTAATELAPA
                     TEDAPPAARLREAGSVILGKTTMPDYGMLTSALSSFHLLTRNPWDLSKNPGGSSAGAG
                     AGAAAGYGPLHVGTDIGGSVRLPASYCGIFGLKPSLGRVPIKPPYPGRVAGPMTRSVR
                     DAALMMEVLSRPDARDHMSLPAQRIEWHSLERDLKGLRLGLLMDAGWGLAVDAEVSSA
                     VLAAAQAFSAAGAIVEPLKPWSTREMLEGIDAFWRMRSWLDMAALPEERRAKVLPYIR
                     AWAEGAASLSGADVFRGYSQMLVLREASVAATQAYDYVLSPVSPIPAFAAELPGPTND
                     PARPLEHIAFTLPYNMSEQPAAAINCGYTAGGLPIGLQIAGRRFDDLGVLQVAYAWEL
                     MRPEQRPWPQRPAA"
     gene            4235..5719
                     /locus_tag="VFV25_07015"
     CDS             4235..5719
                     /locus_tag="VFV25_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007587975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY1393109.1"
                     /translation="MPEAIPFETRQIFVGGRWCACSSGETLPLINPSDGTTLAEIARG
                     GPADIDAAVSAARGALQGTWGRMSAAERGRVLQAMSQRVLAQADELARLEALDVGKAL
                     RQGRADALALARYLEFYAGAADKVHGQTLPYQRGTTALTLREPHGVTGHIVPWNYPMQ
                     IIGRSVGAALAMGNACVLKPAEEACLTALAFTRIAAEAGLPDGALNVVPGLGEEAGAA
                     LSSHHDVNHVSFTGSVATGAMVQAAAARHAVPVTLELGGKSPQIVFADADLDAALPYL
                     VNAGIQNAGQTCSAASRILVERPVYEAVRSRMAERYRALRAGPALRDLDLGPVISARQ
                     KGIVERYLDLARTSGLRLAAQGEIAADAPAGGCYVRPSLLDAVPPDHELAQQEIFGPV
                     QVLIAFDGENEALRIANGTPYGLVAGVWTRDGARQWRMAHGLRCGQVFINNYGAGGGI
                     ELPFGGVKLSGHGREKGFEALYGFSTLKTVAIHHGLAGDGGESA"
     gene            5716..>6111
                     /locus_tag="VFV25_07020"
     CDS             5716..>6111
                     /locus_tag="VFV25_07020"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY1393110.1"
                     /translation="MKLKDKIAIVTGGGSGFGEGIARKFVAEGAQVLIADRDLDAGLR
                     VAASLGPAARALRVDVSLAADVSVMIEAAWEHFGGLDILVNNAGIGHLPQPLENLAEE
                     EFDRLYAVSMKAIYLAARAAVPHFKERGRG"
BASE COUNT          945 a         2117 c         2059 g          990 t
ORIGIN      
        1 gcactcccaa caaagcatga tcgggttcca gcagcctgat cttgcgcgca gtgatctcct
       61 tgatcgtcca ggcccgctcg atgagctggc gaaagtcctt gccgtcgcgg ttcacggccc
      121 ataagccgtt cccacgcaag aagccggaag cgcggtcagt gtcggcaacc gtgagtacaa
      181 ggcgctcgtc gttcagccat tggaagtggt tgatatcagc gtctgaaaag ccagccacga
      241 caaccgccgg tctgtcgcga ctgtccagat cgatgacgcc cagctggata cgcccggtca
      301 tattgcccac gccaaccgcc agatagcgtc ccgacggcga aagctctgcg tcagaaatcg
      361 cgggcgcgcg gaaaaagtcg tcgaccgtgg gctgggcccg tgccacgggg gccgcagaca
      421 cagcagcagc caacaccacg gcccgcagcg caagccagcg cttgatgaat atcttcattt
      481 ctccgactcc ctgtaaatgt ttccggcaaa tttacaaggc ggccatagcc gaggcgagcg
      541 cagtaacccc aagggggcag ggtcatggtc ccccacctac actgcggcat gcctttcaac
      601 cacctgctgc tggccctggc cgtggtcttc gtctggggca ccaatttcgt cgtcatcaag
      661 ctgggcttgg ccgagttgcc accctttttg ttcgcgacgc tgcgcttcgc cgcttcggcc
      721 tttcccttcc tgttcttcat caagcggccg gcggtgccgc tggccaaact ggctgccttc
      781 ggagccttgc tgggcgccgg gcagttcggc ctgctgttct acgcgatgcg cgccgacatt
      841 tcgcccggcc tggcctcgct ggtgatccag acgcaggttt tcttcacgat aggcatggca
      901 atgctgctgc atcgcgagcg gcccagggcg gaacagggca tcgcgctggc gctggccgtg
      961 gccggcatgg gcgtgatcgc ggcgcatctc aacggctcga tcacggcctt gggcctggtg
     1021 ctggtgactt ttgccgcctt gagctgggcc tgcgcgaacc tggtcgtcaa atcggccggc
     1081 cgagtggaca tgctgggctt catggtctgg tccagcatct tcgccgtacc tccactgctg
     1141 gttctgagtc tggtcttcga agggcccacc gcgatcgcgc agggcctggc acacgccgac
     1201 tgggtcggct gggccgctgt ggcctggcaa gcggtgggca acaccctgtt cggctacgga
     1261 gcctggaact ggctgctggc ccgccacgcc gccgccaccg tgacgccgat ggcgctgctc
     1321 gtaccagtgt tcggcatggc ggcctcggcg ctcgccttct ctgaagcaat gccggcctgg
     1381 aagctgctcg ccgcggcgct ggtgctgggc gggctggccc tcaatgtcgc ggccagccgc
     1441 atcgcgctgc gctcgctgca aaccgctaga ggctgatgac caccatgacg atccgcctgc
     1501 actactaccc gagcaatgcc agcatggcac cgcacatgct gctcgaggaa ctcggcctgc
     1561 gctatgagct ggtgctggtc gaccgcacgg tgggggcgca caagtcgccc gaatacctca
     1621 agctcaatcc caatggcctg atcccggtac tggaagacgg cggcctcgtg ctttacgaaa
     1681 cggccgcgat ctgcctgcat ctggtggaca gccacccgca tgccggcctc gcgccggccc
     1741 tgggcacgcc gcagcgcgcg catttctaca agtggctgat gtggctcacc aacacgctgc
     1801 aggcttcgct cagcgtctac ttctatccgg agcgctgggc tgatggcgac gatgcgatcg
     1861 cgcaggtgcg agcccatgcc gagaccagga tcgtcggcct gctccagcag ctcgacgccg
     1921 agctcgcccg ccacggcggc ccctggctgc tgggcgaaga ctacagcgcg ctcgacccct
     1981 atgcgctgat gctgtgccgc tggacgcgcg gtttcgaaaa gcccgcgcgc agcctgccgg
     2041 cactcggccc ttatctgcag cgcgtactgg cgcggcccgc gatacgccgg gccttcgagc
     2101 aggagcggct gccgctgccc tggatttgat ggcgcagcga ctacgcgact gcggctaggc
     2161 gcagcagccc ttcccctcat cccccatctg caccggcggg catttcacgc tgccgtagga
     2221 gcagaacacg cagcaatcgc ccggcctcgg ccgcagcagc gcgccgcaga gcctgcattc
     2281 gtagaagtag acgcaggcat cggtaggcat ctgctccagc gatctgccgc cgcagtgggg
     2341 gcatgtcagc tcggagcgca gctcgatttc agaggatgtc gtcacgatgg cctcggtctt
     2401 acagtgcgcc ggccctgcta agcttctcga gccgcagcgc gatgtcctgc tgcagctcgc
     2461 tgatgcgccg cgtcaacttc ggctcctctt ctgcactaac acccgctgcc agtcgcctcc
     2521 tcagctcggc cagctcgcgc agcatcgtca cctcgtgcgg cacgaagcct gcatccttca
     2581 agaccttgaa gggcatgcgc agctcgatcg gcgtctcgtc gtagccgtcg ccgaagtcca
     2641 gcggccggcc atagctgggc gcggcacgca gttcgccgtt ggcttcgctt tcgctcaagc
     2701 cttgcgcgat caactcgtcc agcgtgggca tgcgcctttc tttcgcctca ggccgccggc
     2761 cgctgcggcc acggccgctg ctcgggccgc atcagctccc aagcataggc cacctgcagc
     2821 acgcccaggt cgtcgaagcg gcggcccgcg atctgcaggc cgatcggcaa gccgccggcc
     2881 gtgtagccgc agttgatagc cgcggccggc tgctcgctca tgttgtaggg cagcgtgaag
     2941 gcgatgtgct ccagcggccg cgcggggtcg ttggtcggac cgggcagctc agccgcgaaa
     3001 gccgggatgg gcgagaccgg cgacagcacg tagtcgtagg cctgcgtcgc ggcgacgctt
     3061 gcctcgcgca gcaccagcat ctggctgtag ccgcggaaca cgtccgcgcc gctcagggat
     3121 gcagcccctt cggcccaggc gcggatatag ggcagcacct tggcgcggcg ctcttcgggc
     3181 agcgcggcca tgtccagcca ggagcgcatg cgccagaagg cgtcgatgcc ttcgagcatc
     3241 tcgcgcgtgc tccaaggctt cagcggctcg acgatcgcgc ctgccgcgct gaaagcctgc
     3301 gcggcggcga gcaccgccga actcacctcc gcatccaccg ccagacccca gccggcatcc
     3361 atcagcaggc ccagcctgag gcccttgaga tcgcgctcca ggctatgcca ttcgatgcgc
     3421 tgcgcgggca ggctcatgtg gtcgcgcgca tcggggcggc tcagcacttc catcatcagc
     3481 gccgcatcgc gcacgctgcg cgtcatcggc ccggcgacgc ggcctggata gggcggcttg
     3541 atcggcacgc ggccgaggct gggcttgagc ccgaagatgc cgcaatagct ggccggcaga
     3601 cgcaccgagc cgccgatgtc ggtgcccaca tgcagcggcc catagccggc cgcggcgccg
     3661 gcacccgcgc ctgcgctgga gccgccgggg ttcttgctca ggtcccaggg attgcgcgtc
     3721 agcaggtgga agctggatag ggccgaggtc agcatgccgt agtcgggcat cgtggtcttg
     3781 ccgaggatga cggaaccggc ctcgcgcaac cgagcggccg gcggcgcgtc ctcggtcgcc
     3841 ggcgccagct cggtggcggc cgtgccgagc ggcaccggca cgccgcgcgt ggcgatgttc
     3901 tccttgatca tggccggcac gccgtccagc gccgacagcg gctggcccgc atgccagcgc
     3961 cgctcggacg cttgcgccat cacaagcgcc gcatcgggat cgaaggcgta ggtcgccctc
     4021 agcttcggct cccaggcctc gatatgcgca atgacggctc tcgccgcctc gaccggcgag
     4081 agccggccct gggcatagag cgtattgagc tcgacggcgc tgagatcgtg cagctcctgc
     4141 atggtttccc cgatcgatgg tcctggcagc gcgacgcagc gtatcctgtt gcccctgggc
     4201 acacaagccg cggcaggaga acgaggagac agccatgccc gaagccatcc ccttcgagac
     4261 gcgtcagatc ttcgtcggcg ggcgttggtg tgcatgcagc agcggcgaga cgctgccctt
     4321 gatcaacccc tcggacggta cgacgctggc cgagatcgca cgcggcgggc cggccgatat
     4381 cgacgccgcg gtgagcgcgg cgcgcggtgc gctccaaggc acttggggcc gcatgagcgc
     4441 ggccgagcgc ggcagggtac tgcaggccat gagtcagcgc gtgctggccc aggccgacga
     4501 gttggcgcgg ctggaggcac tggacgtcgg caaggccttg cggcagggtc gcgccgatgc
     4561 gctggcgctg gcgcgctacc tggagttcta tgccggcgca gccgacaagg tccatggcca
     4621 gaccctgccc taccagcgcg gcaccacggc gctgacgctg cgcgagccgc atggcgtgac
     4681 cggccacatc gtgccctgga actacccgat gcagatcatc ggccgcagcg tcggcgcagc
     4741 gctggcgatg ggcaatgcct gcgtgctcaa gccagccgag gaggcttgcc tgaccgcgct
     4801 cgccttcacg cgcatcgccg ccgaggctgg gctgcccgac ggcgcgctca atgtggtgcc
     4861 gggcctgggc gaagaggccg gcgcggcgct cagctcgcac cacgacgtga accacgtctc
     4921 cttcaccggc tcggtcgcca ccggcgcgat ggtgcaggcg gccgcggccc gccacgccgt
     4981 gccggtgacg ctggagctgg gcggcaagag cccgcagatc gtgttcgccg atgccgacct
     5041 cgacgcggcc ctgccctatc tcgtcaacgc gggcatccag aacgccggcc agacctgctc
     5101 ggccgcctcg cgcatcctgg tcgagcggcc ggtctatgaa gcggtgcgca gccgcatggc
     5161 cgagcgctat cgcgcgctgc gcgccgggcc ggcactgcgc gacctggacc tcggcccggt
     5221 catttcggcg cggcagaagg gcatcgtgga gcgatatctc gatctcgcgc gcaccagcgg
     5281 gctgcggctc gctgcgcaag gtgagatcgc ggcagatgcc cctgcgggcg gctgctatgt
     5341 acgccccagc ctgctggatg ccgtgcctcc cgaccacgag ctggcgcaac aggagatctt
     5401 cggcccggtg caggtgctga tcgccttcga cggcgagaac gaagccctgc gcatcgccaa
     5461 cggcacgccc tatggcctgg tggccggcgt gtggacacgc gacggcgcgc ggcaatggcg
     5521 catggctcac ggcctgcgct gtggccaagt cttcatcaac aactacggcg cgggcggcgg
     5581 catcgaactg cccttcggcg gcgtcaagct ctcgggccat gggcgcgaga agggcttcga
     5641 ggcgctgtac ggcttctcga cgctgaagac ggtggcgatc caccatgggc tggcaggcga
     5701 cggaggagaa agcgcatgaa actcaaggac aagatcgcca tcgtcaccgg cggcggctcc
     5761 ggcttcggcg agggcatcgc gcgcaagttc gtcgccgaag gtgcccaggt gctgatcgcc
     5821 gaccgcgatc tggacgcagg gctgcgcgtc gcggcctcgc tggggccggc ggcgcgggcg
     5881 ctgcgcgtgg acgtgagcct ggcggccgac gtgagcgtga tgatcgaagc ggcgtgggag
     5941 catttcggag ggttggacat tttggtcaac aacgccggca tcggccacct gccgcagccg
     6001 ctggaaaacc tcgcggaaga ggagttcgac cgcctctatg cggtcagcat gaaggcgatc
     6061 tatctggccg cacgtgccgc cgtaccccat ttcaaggagc gcgggcgcgg c
//
LOCUS       DASUCY010000355         3626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_650218, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000355 DASUCY010000000
VERSION     DASUCY010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650218"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VFV25_07025"
     CDS             complement(<1..303)
                     /locus_tag="VFV25_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY1393111.1"
                     /translation="MSRSIQTLFDLKGRTALVTGGSRGLGLQIAESLGEAGAKIMLSS
                     RKAADLEESAAHLQAKGIDTRWIAADASQPSEIERIANETLERLGQVDILVNNAGAT"
     gene            complement(355..564)
                     /locus_tag="VFV25_07030"
     CDS             complement(355..564)
                     /locus_tag="VFV25_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393112.1"
                     /translation="MKPSTLDVWVWVLIYGGLLAVSLGVFVARQDEALGTFFGLGGGI
                     ASAIGLVLIYVRSRIKDGPGNGAKQ"
     gene            complement(602..2425)
                     /locus_tag="VFV25_07035"
     CDS             complement(602..2425)
                     /locus_tag="VFV25_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013802767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEY1393113.1"
                     /translation="MHMHATLDFLLYDWLRVDDLTTRARFADHSRETFDSVLDTCERI
                     AREKFAPFNRLCDTEEPHFDGERVRLPEATREAVRAYADSGMLAASQPYEVGGMQLPC
                     VVEMAANSFFSMASVAMGGYAMLTSGNASLLMAHGTPAQREVFAKNEFSGRWFGTMCL
                     SEPQAGSSLSDVATRAVPDGADFESDALGPRYRLRGNKMWISAGEHELTENIIHLVLA
                     KIPGADGRLPPGVKGISLFAVPKKLVDAKGELTGERNDVALAGLNHKLGYRGTTNTLL
                     NFGEGKFPVRGAAGAIGYLIGKPGEGLRCMFHMMNEARIGVGLGATMLGYAGYLAALE
                     YAKQRPQGRPVGAGGKDAGAPQVPIIEHADVKRMLLAQKAYCEGALALELYCARLVDE
                     QQTGTPEQASDARLLLEVLTPIAKSWPSEWCLEANSLAIQVHGGYGYTRDFPVEQYWR
                     DNRLNMIHEGTHGIQALDLLGRKVVMEGGQGLMKLAGHMNETIEQALHVAELAEFANS
                     LAAALKRLGNATKAAWATGVPEEALANATPYLQAFGHTVLAWIWLELALAARRGSTDF
                     HRGKLAAARYFFRYELPRTEAWLGVVASRDATCRETLAAWF"
     gene            complement(2457..3323)
                     /locus_tag="VFV25_07040"
     CDS             complement(2457..3323)
                     /locus_tag="VFV25_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393114.1"
                     /translation="MKLEATRARKVDQLQRASSSLSKRWLVALSLSAPLLAGAATPGQ
                     YSLEAIGRLSCEDSVKRLEEGMAAKDGEAFFTAGKLYDEGACVSRDAAKAVELWRNAL
                     ALGQTQAAASLAVNVGLGQGAAQDYAAAGALAKQAGVRMGAEADAADYSLGYAYTWWQ
                     LTQREFQYPQQLIASDVRGSAELRFEPKSGSWKLVSFRRSGNSGETEVGSRIDRSKSL
                     VSQAVSEAARAAQDKLAKPDAARVDQLSYAGKLVVAPPGDASERAEDLQRFLRIQNVQ
                     ELRQPTRMGGGG"
     gene            complement(3474..>3626)
                     /locus_tag="VFV25_07045"
     CDS             complement(3474..>3626)
                     /locus_tag="VFV25_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019042205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEY1393115.1"
                     /translation="AIAVGHPYGVSGQRLTGHALIEGKRRGAKRVVVTMCIGGGMGAA
                     GVFEVL"
BASE COUNT          571 a         1263 c         1173 g          619 t
ORIGIN      
        1 agtagcgccg gcgttgttga ccaggatgtc cacctggccg aggcgctcca gcgtctcgtt
       61 ggcgatgcgc tcgatctccg acggctgact cgcatcggcc gcgatccagc gggtgtcgat
      121 gcccttggcc tgcaggtgtg cggccgactc ctcgaggtca gccgccttgc gcgagctcag
      181 catgatcttg gcgccggcct cgcccaggct ctcggcaatc tgcaggccga gcccgcgcga
      241 gccgccagtg acgagcgcgg tgcgcccctt gaggtcgaag agggtctgga tgctgcggct
      301 catggctgag ttcctgaaga agaaatgtct gaaggtgggc gctagtgctt ggcgctattg
      361 cttggcaccg ttgcccggcc catctttgat gcgcgatctc acatagatca gcaccaagcc
      421 gatggcggat gcaatgccgc cgcccaggcc aaagaaggtg cccagggctt catcctgccg
      481 ggccacgaag acgcccaggc tcacggccag gaggccgccg tagatgagga cccagaccca
      541 tacatcgagc gtcgacggtt tcatgtggct gcggctccgc tgtggcgttg cgatggtgga
      601 ttcagaacca tgcggccagg gtctcacggc aggtcgcgtc tcgcgaggcg accacgccca
      661 gccaggcttc ggtcctgggc agctcgtagc ggaagaagta gcgagccgca gcaagcttgc
      721 ctcggtggaa atcggtgctg ccccgccgcg cggcgagcgc cagctcgagc cagatccagg
      781 ccagcaccgt gtggccaaag gcctgcagat agggcgtcgc gttggccagc gcctcctcgg
      841 gcacgccagt cgcccaggcc gccttggtgg cattgcccaa gcgcttgagc gccgcggcga
      901 ggctgttcgc gaactcggcc agttcggcca cgtgcagggc ctgctcgatg gtctcgttca
      961 tgtggccggc cagcttcatc aggccctgtc cgccttccat cacgaccttg cggcccagca
     1021 ggtccagcgc ctggatgcca tgcgtgccct catggatcat gttgaggcgg ttgtcgcgcc
     1081 agtactgctc gaccgggaag tcgcgcgtat agccgtagcc gccatgcacc tggatcgcga
     1141 ggctgttggc ctccaggcac cattcgctgg gccagctctt ggcaatcggc gtgagcacct
     1201 ctagcagcag ccgcgcgtcg ctcgcctgct caggcgtgcc ggtctgctgc tcatccacca
     1261 ggcgcgcgca gtacagctcc agcgccagcg cgccttcgca atacgccttc tgcgccagca
     1321 gcatgcgctt gacatcggca tgctcgatga tgggcacctg cggcgcgccg gcgtccttgc
     1381 cgccagcgcc gaccggccga ccctgcgggc gctgctttgc atactcgagc gcagcgagat
     1441 agcccgcgta gcccagcatc gtggcgccca ggccgacgcc gatgcgcgcc tcgttcatca
     1501 tatggaacat gcagcgcagg ccctcgccgg gcttgccgat gaggtagccg atggcgccgg
     1561 ccgcgccccg caccgggaac ttgccctcgc cgaaattgag cagcgtgttg gtcgtcccgc
     1621 gatagccgag cttgtggttc agccctgcca gcgcgacatc gttccgctcg ccggtcaact
     1681 cgcctttcgc gtcgacgagc ttcttcggca cggcgaagag ggagatgccc ttgacgcccg
     1741 gcggcagcct gccatcggcg ccggggatct tcgccagcac gaggtggatg atgttctcgg
     1801 tcagctcgtg ctcgccggcc gagatccaca tcttgttgcc tctgaggcga tagcgcggcc
     1861 ccagcgcgtc ggactcgaaa tccgcgccgt cgggcacggc gcgcgtcgcc acgtccgaga
     1921 ggctggagcc ggcctgcggc tcggacaggc acatggtgcc gaaccagcgc ccagagaact
     1981 cgttcttcgc gaacacctcg cgctgcgccg gcgtgccatg cgccatcagc agactggcgt
     2041 tgccgctggt gagcatcgca tagccgccca tcgccacgct ggccatcgag aagaagctgt
     2101 tggccgccat ctcgacgacg cagggcagct gcatgccgcc gacctcgtag ggctgcgagg
     2161 cggcgagcat gccggagtcg gcataggcac gcaccgcttc acgcgtggct tcgggcaggc
     2221 gcacccgctc gccgtcgaaa tgcggctcct cggtatcgca gagccggttg aagggggcga
     2281 acttctcgcg cgcgatgcgc tcgcaggtgt ccagcaccga gtcgaaggtc tcgcgcgagt
     2341 ggtcggcgaa gcgcgcgcgg gtggtcaggt cgtcgaccct caaccagtcg tagagcagaa
     2401 agtccagcgt cgcgtgcatg tgcatcaagc ccggtccagt tcgccctcag cagctttcag
     2461 ccgccgccac ccatcctagt gggctgcctg agctcctgga cgttctggat ccgcaggaag
     2521 cgctgcaaat cttcggcccg ctcggatgca tcccccggcg gcgcaacgac aagcttgccg
     2581 gcatagctga gctggtccac gcgcgcggcg tccggcttgg cgagcttgtc ctgggccgcg
     2641 cgcgccgcct cggatacggc ttgcgaaacc agggatttgg aacggtcgat ccgcgatccc
     2701 acttcggtct ccccactgtt gccgctgcgc cggaaggaca cgagcttcca gctaccgctc
     2761 ttgggctcga agcgcaactc ggcggatccg cgcacatcgg aagcgatcag ttgctgcgga
     2821 tactggaact cgcgttgcgt gagctgccac caggtgtagg cgtagccgag gctatagtcc
     2881 gcggcatcgg cttccgcgcc catgcgcacg cccgcctgct tggccagggc gcccgctgcc
     2941 gcatagtcct gcgcggcgcc ttgtcccagg cccacgttca ctgccagcga agcagccgcc
     3001 tgcgtctgac cgagggccag cgcgttgcgc cacagctcga ccgccttcgc cgcatcgcgg
     3061 gaaacgcaag ctccttcgtc atagagcttc ccggcagtga agaaagcctc gccatccttg
     3121 gccgccatgc cttcttcaag ccgtttgacg ctgtcttcgc aggacagcct gcctatcgcc
     3181 tcgaggctgt actgcccggg cgtcgccgcg ccagccagca gcggcgcact caggctgagc
     3241 gcgacaagcc aacgcttgct cagcgacgac gacgctctct ggagctggtc gacttttctt
     3301 gctcttgttg cttccaattt catagcaggg aggctcccta aagatggttc atggagcagc
     3361 tcggcccagc ccctgtctcg cctcgtgaac gaacgacccg aagagtgggc acgcgactgc
     3421 aagcggacac ggcatgtagc gacaaccgcg cgtccagccg tgaagcgccg aggttacagc
     3481 acctcgaaca cgcccgcagc ccccatgccc ccgcctatgc acatcgtgac cacgacgcgc
     3541 ttggccccac ggcgcttgcc ttcgatcagc gcgtggccgg tcaggcgctg gccgctcacg
     3601 ccataggggt ggccgaccgc gatcgc
//
LOCUS       DASUCY010000356         3908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_634289, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000356 DASUCY010000000
VERSION     DASUCY010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634289"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..123)
                     /locus_tag="VFV25_07050"
     CDS             complement(<1..123)
                     /locus_tag="VFV25_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005014546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY1393116.1"
                     /translation="MNVRPIYLDYAATTPVDPRVVHAMVPYLYEQFGNPASRSHA"
     gene            438..935
                     /locus_tag="VFV25_07055"
     CDS             438..935
                     /locus_tag="VFV25_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393117.1"
                     /translation="MNLFTGAVADEFFGGDVPASVRGILHRAAQSPRDEVGSLLWTAQ
                     ALAPTCLPIYYALYKHHASRRELEMAQRAARRGLLESASQAGLPQNWRSVEPGMPAAG
                     FGNNGPARFWLFTLKALAFLALRQHKVGEARELLAQIARLDPAARIGDDVIASLLASM
                     GPDRS"
     gene            complement(940..1281)
                     /locus_tag="VFV25_07060"
     CDS             complement(940..1281)
                     /locus_tag="VFV25_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393118.1"
                     /translation="MLQVIGETGESVLTLTEAVEEAELMRSRLTRPEVLRQLRTLADT
                     LEALPQSVREQMPEIDWAGWRAARRAFHEEHGTALNSVLWFAVKSLVPATLSWLRVYR
                     KSHPQLFNSWG"
     gene            1347..1919
                     /locus_tag="VFV25_07065"
     CDS             1347..1919
                     /locus_tag="VFV25_07065"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY1393119.1"
                     /translation="MKQELHSARLLLRPPRPGDEHAAHALWASDPQVLRYLHWKPHTQ
                     LAQTRSQLAWEQARWIKRSAWTWLIVPLHKPVGEQQLQPIGLVQLTPQQRDGEAHHLR
                     LGYLLARRHWGRGLMREALDVVLAQAFSQAAVWRIDAVCDLENVASERVLRACGFRLE
                     GVLARYSLHPNRGTQPRDAALYARVRSAGP"
     gene            1962..2195
                     /locus_tag="VFV25_07070"
     CDS             1962..2195
                     /locus_tag="VFV25_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393120.1"
                     /translation="MHSDPTPLRAFAKAANYEVRFASLFQAGRALSFPCDAQGSVLLE
                     VLNERARTAYGRAVALVGSEYATPVVWCADPRG"
     gene            complement(2192..3028)
                     /gene="modD"
                     /locus_tag="VFV25_07075"
     CDS             complement(2192..3028)
                     /gene="modD"
                     /locus_tag="VFV25_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HEY1393121.1"
                     /translation="MERLPDEMLQRWLHEDAPHGDLTTRALGISGAPARLQFHARGAM
                     RVALIEEAVRLFELCGATANLHARSGSDAAPGALLLDAAGPANCLLLAWKVAQTAVEV
                     ASGIAGETARIIGLLRAAGHAQALACTRKTMPGSRALAVRAVLAGGGIMHRLGLSESL
                     LVFPEHRLYLDVATLHERLAAIRAAQPEKRLVVEVGSEEDALSLAAAGAEVLQLERFT
                     PEALRRLKETLRSHSLHPLLAPAGGVTAANAVAYAEAGADFLVSSAPYHAAPADVQVR
                     FG"
     gene            complement(3034..3399)
                     /locus_tag="VFV25_07080"
     CDS             complement(3034..3399)
                     /locus_tag="VFV25_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393122.1"
                     /translation="MDREHDSWQARLPAELQASVRWPVRVESHHDDGVPASKWRGYDA
                     LGQLCYYRHHYAQWDYTFDDEDVPCMRMLRSEHFEAWRCLDGRWLRRVQAVLGERCCT
                     GGETDSGFVIVPPQQIPRL"
     gene            complement(3425..>3908)
                     /locus_tag="VFV25_07085"
     CDS             complement(3425..>3908)
                     /locus_tag="VFV25_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HEY1393123.1"
                     /translation="ESGRVRAAGPLTELLARADLPLAGADDGGVVLDACVLEHDTRFG
                     LTRIAVEGATLWVGALEQPLGSAVRARVLARDVSIVRSVPADSSILNVLPVTLRGWHC
                     DATTVLLSLALGHADARAEGLPRSGGLLARITRRSFETLQLRQDEALHAQIKGVSLMR
                     "
BASE COUNT          643 a         1346 c         1276 g          643 t
ORIGIN      
        1 ggcatggctg cgcgaggccg gattaccgaa ctgctcgtac agatagggca ccatggcatg
       61 cacgaccctc gggtccacgg gcgtggtggc ggcgtagtcc agatagatcg gtctgacatt
      121 cagcttcatg tccatcgcac gactccggct taggggtacg acgctgacat ctcaaggatc
      181 gtgccagatc ccgcgctttg ttgatgcagc gcaaaaaagc cgccgtggat caggcacttg
      241 gccagtttcg ggcttcggcg cgcgccgttc tccggtccca gcggctgtcg cggccctgtc
      301 gcttttgtcg gaacaccgac gcgacgcaag cgttgtcgag gcggtctttc ccagccaggc
      361 agggctttcg gctcctggca tcaagactgc ttgtgagcct cgtgtattaa acacgtattt
      421 gccgcaggag ccgcgagatg aatctgttca ccggcgctgt cgccgacgag ttcttcggcg
      481 gcgacgtgcc cgccagcgtg cgcgggatcc tgcaccgcgc ggcgcagtcg ccacgcgacg
      541 aggtaggctc cctgctgtgg accgcgcagg ccctcgctcc tacatgcctg ccgatctatt
      601 acgcgctcta caagcaccac gcttcgcggc gcgagctgga gatggcgcag cgcgccgcgc
      661 ggcgcggtct gctcgaatcg gcaagccagg ccggcctgcc tcagaactgg cgctcggtgg
      721 agccgggcat gcccgcagca ggcttcggca acaacggccc cgcgcgcttc tggctgttca
      781 cgctcaaggc gctggccttc ctcgcgctac gccagcacaa ggtgggcgaa gcgcgggaac
      841 tgctcgcgca gatcgcgcgc ctcgatccag ccgcacgcat cggcgacgac gtcatcgcct
      901 cgctgctcgc ctcgatgggg ccggatcgct cctgagcttt cagccccacg agttgaagag
      961 ttgcggatgc gattttcgat acacgcgcag ccagctcagc gtggccggca ccagcgactt
     1021 cacggcgaac cacaagacgc tgttcagcgc cgtcccgtgc tcctcgtgaa aggcgcgtcg
     1081 cgcggcacgc cagccggccc agtcgatctc cggcatttgc tcgcgcacgg attggggcaa
     1141 ggcttccagc gtgtcggcaa gcgtgcgcaa ctggcgcagt acctcggggc gcgtgagccg
     1201 cgagcgcatg agttcggctt cttcgacggc ttctgtcaag gtcagcacgg actcaccggt
     1261 ttcgccgatc acctggagca tgggagcgat catcatgatg agcacatcct tgcagctttc
     1321 gcgccatgcg cgggccggca cctgcaatga aacaggagct gcacagcgcg cgactactgc
     1381 tgcgccctcc gcggccgggc gacgagcatg ccgcgcacgc gctgtgggcc agcgacccgc
     1441 aagtgctgcg ctacctgcat tggaagccgc atacgcagct cgcacagacg cgctcccagc
     1501 tggcctggga gcaggcgcgc tggatcaagc gcagcgcctg gacctggctg atagtgccgc
     1561 tgcacaagcc ggtgggcgaa caacaactgc aacccatcgg gctcgtgcag ctgacaccgc
     1621 aacagcggga cggagaggcc caccacctgc gcctgggcta cttgctcgcc cgcaggcatt
     1681 gggggcgcgg gctgatgcgc gaggcgctcg atgtagtgct tgcgcaagcc ttctcgcagg
     1741 cggcggtttg gcgcatcgat gcggtgtgcg atctcgaaaa cgtggcctcg gaacgcgtgc
     1801 tgcgggcctg cggcttcagg ctggaaggcg tgctggcgcg ctacagcctg catcccaacc
     1861 ggggcacgca accacgtgat gctgcactct atgcgcgagt gcgcagtgct ggcccatgac
     1921 ttgctgtgta cctcctgtcg ccagcttcag gagccgttgt gatgcatagc gatccgaccc
     1981 cattgcgtgc cttcgccaag gcagcgaact acgaggtgag gtttgcgtcc ctgttccagg
     2041 ccggccgcgc gctgagtttt ccctgcgatg cgcaaggcag cgtgctgctc gaagtcttga
     2101 acgagcgcgc ccggactgct tacggccgtg cagtggcgtt ggtgggcagc gagtacgcaa
     2161 ccccggtggt gtggtgtgcc gatccgcgtg gctaaccgaa ccttacctgc acatcggccg
     2221 gcgccgcgtg gtagggcgcc gagctgacga gaaagtccgc gcctgcttcg gcataggcaa
     2281 ctgcattcgc ggccgtgacg ccgccggccg gcgccagcaa cgggtgcagg gaatgtgatc
     2341 gcagcgtttc cttcaagcgg cgcagcgctt caggcgtgaa gcgctccagc tgcagcactt
     2401 cggcgcccgc ggcggccagc gacagcgcgt cctcttcgct gcccacctcg accaccagcc
     2461 gcttctccgg ctgcgcggcg cgtatggctg ccagccgctc gtgcagggtt gcgacatcaa
     2521 ggtacagccg gtgttccgga aacacgagca aggattctga caagcccagg cgatgcatga
     2581 tgccgccgcc tgccagcacc gcacgcactg ccaatgcacg gctgcccggc atggtcttgc
     2641 gtgtgcacgc cagggcctgg gcatggccag cggcgcgcag cagaccgatg atgcgcgccg
     2701 tctcgccggc gatgccactg gctacctcga ccgccgtctg cgccaccttc catgcgagta
     2761 gcaggcagtt cgccgggcct gcggcatcga gcagcagcgc gcccggcgcg gcatccgagc
     2821 cgctgcgcgc atgcaggttt gccgtggcgc cgcacaactc gaacaagcga acggcctcct
     2881 cgatcagcgc cacgcgcatc gcgccgcgcg catggaactg cagccgcgcc ggcgcgccgc
     2941 tgatgcccag cgcgcgagta gtcagatcgc catgcggcgc gtcctcgtgc agccagcgct
     3001 gcagcatctc gtcagggagg cgttccatat gcctcacaac ctcgggatct gctgcggcgg
     3061 cacgatcacg aaaccgctgt cggtctcgcc gcccgtgcag catcgctcgc cgaggaccgc
     3121 ctgcaccctg cgtaaccaac ggccatccag gcaacgccag gcctcgaagt gctcgctgcg
     3181 aagcatgcgc atgcagggca cgtcctcgtc gtcgaaggtg tagtcccact gggcatagtg
     3241 gtggcggtag tagcacagct gacccagcgc gtcatagcct cgccacttcg aggccggcac
     3301 gccatcgtcg tggtgggact ccacgcgcac gggccagcgg acactggcct gcaactcggc
     3361 aggcaggcgg gcttgccagg aatcgtgttc tctgtccatt gttctaactt ccttcttctt
     3421 ttaatcagcg catcagcgac acgcccttga tctgcgcatg cagggcttca tcctgccgca
     3481 gctgcagcgt ctcgaacgag cggcgagtga tgcgcgccag caggccaccc gaccgtggca
     3541 aaccctccgc tcgggcgtca gcgtggccca gggccaggct gagcagcacg gtcgtggcat
     3601 cgcaatgcca gccccgcagc gtgaccggca gcacgttgag gatgctgctg tcggcaggaa
     3661 cgctgcgcac gatgctcacg tcgcgcgcca gcacccgggc gcgcaccgcg ctgcccaggg
     3721 gctgctcaag ggcaccgacc cagagtgtgg ccccctcaac ggcaatgcgc gtgaggccaa
     3781 agcgtgtatc gtgttccagc acgcaggcgt ctagcaccac gccgccatcg tccgcaccgg
     3841 ccaagggcag atcggctcgc gcaagcaact cggtcaaggg cccggccgcg cgcacacggc
     3901 cggactcc
//
LOCUS       DASUCY010000357         3609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_939460, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000357 DASUCY010000000
VERSION     DASUCY010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939460"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..159)
                     /locus_tag="VFV25_07090"
     CDS             complement(<1..159)
                     /locus_tag="VFV25_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HEY1393124.1"
                     /translation="MNPPLLPKIDSPADLRHLSRAELQQLATELRDYVLQSVSQTGGH
                     LSSNLGTVE"
     gene            complement(204..1094)
                     /locus_tag="VFV25_07095"
     CDS             complement(204..1094)
                     /locus_tag="VFV25_07095"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEY1393125.1"
                     /translation="MKLEAYESWARARLEAVEATLDRLVPAKTPAGLGEAMRYGVLDG
                     GKRLRPLLVLAAAEAVHAHGEASLRAACAVELIHAYSLVHDDMPCMDNDVLRRGKPTV
                     HVKYGEAQAMLAGDAMQALAFEVLTPEDDSVAPALQARLCGLLARAAGHAGMASGQAI
                     DLASVGRPLDEHELRDMHRRKTGALLQASVLMGAACGEPGETAWRALSDYGAALGLAF
                     QVVDDILDVTQASETLGKTAGKDADNNKPTYVSLLGLDAARHHAHELREQAHAALARS
                     GLRDAAWLSLLADKVVDRDS"
     gene            complement(1114..1356)
                     /locus_tag="VFV25_07100"
     CDS             complement(1114..1356)
                     /locus_tag="VFV25_07100"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEY1393126.1"
                     /translation="MPKNPVSAVASDAPASYEDALAELERLVAAMEGGQLPLDQMLES
                     YKRGAELLSFCRGRLEAVEQQVKVLEAGQLKPWSEA"
     gene            1595..2752
                     /locus_tag="VFV25_07105"
     CDS             1595..2752
                     /locus_tag="VFV25_07105"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEY1393127.1"
                     /translation="MSDLSITLEALERSQTQLGVTSYFDTDLYQRELELIFQPGPRYL
                     GHELCVPEIGDYHTLRQEGEGRTLVRTPEGLQLISNVCRHRQAVMLNGRGNTRSNVVC
                     PLHRWTYDLKGQLVGAPHFADDPCLHLNNYPLQTWNGLVFEGPAKGGQAAARNVAADL
                     AGLGQLADFDFSGYVFDRVHLHHCDYNWKTFIEVYLEDYHVGPFHPGLGNFVTCEDLR
                     WEMGREYSVQTVGVAKSGGQALGKPGSAVYKQWHDVLLDFRRSRHGEEPPKHGAIWLT
                     YYPTVMLEWYPHVLVVSTLYPQGPQKTLNLVEFFYPEEIQAFEREFVEAQQAAYMETC
                     VEDDEIALRMDAGRKALMERGDNEIGPYQSPMEDGMQHFHEWYRRAMRLQA"
     gene            2851..>3609
                     /locus_tag="VFV25_07110"
     CDS             2851..>3609
                     /locus_tag="VFV25_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY1393128.1"
                     /translation="MSAPLLMILASFLFATMGVCVKLASARYSAGEIVMYRGLVGAAL
                     MLVLSRLRGGSLRTAVPTMHFWRSLTGVCSLCLWFYAIGNLPLATAMTLNYMSSVWIA
                     LFLVGGAIMLGGTKVDGRLIGIVLLGFIGVTLILRPTMEHDQLWHGLMGLLSGMMAST
                     AYMQVAALGRAGEPEYRVVFYFSLGGAVAGGLVAVLFGGGLHGHSLSGFALLMAVGLL
                     ATAAQLMITRAYTVGKLLVNASLQYLGIAFSFLYG"
BASE COUNT          543 a         1217 c         1212 g          637 t
ORIGIN      
        1 ctccaccgtg ccaaggttgg acgacagatg accgccggtc tgcgagacgc tttgcagcac
       61 gtagtcgcgc agctcggtgg cgagctgctg cagctcggcg cggctcaggt ggcgcaggtc
      121 ggccgggctg tcgatcttgg gaagcagagg cgggttcatc gtgatttggt gtggttggcg
      181 ccagtacgcg tcgggtttgc gcgtcaggag tcgcggtcga ccaccttgtc ggccagcagg
      241 ctgagccatg cagcgtcgcg cagcccgctg cgggccaacg cggcatgggc ctgttcgcgc
      301 agctcatggg catgatgccg cgcggcatcc agcccgagca gcgagacata ggtcggcttg
      361 ttgttgtcgg cgtccttgcc ggccgtcttg cccagggtct cggaggcctg ggtcacgtcg
      421 aggatgtcgt ccacgacctg gaaggccagg cccagcgcgg cgccgtagtc ggacagcgcg
      481 cgccacgcgg tctcgcccgg ctcgccgcag gccgcgccca tcagcacgct ggcctgcagc
      541 agggcaccgg tcttgcggcg gtgcatgtcg cgcagttcat gctcgtccag cggccggccc
      601 acgctggcca ggtcgatcgc ctggccgctg gccatgccgg cgtggccggc ggcacgcgcc
      661 agcaagccgc acaggcgcgc ctgcagggcg ggcgccacgc tgtcgtcctc gggcgtcagc
      721 acctcgaagg ccagggcctg catcgcatcg ccggccagca tggcctgggc ttcgccgtat
      781 ttcacgtgca ccgtgggctt gccgcggcgc agcacgtcgt tgtccatgca gggcatgtcg
      841 tcatgcacca gcgagtaggc gtggatcagc tcgaccgcgc aggcagcgcg cagcgacgcc
      901 tcgccatgcg cgtgcacggc ctcggccgcg gccagcacga gcagcgggcg caggcgcttg
      961 ccgccgtcga gcacgccata gcgcatcgct tcaccgaggc cggcaggcgt cttggcgggc
     1021 accaagcggt cgagcgtcgc ctccacggct tcgagtcggg cgcgggccca gctttcgtag
     1081 gcttcgagtt tcatcgagat attggctgcg gcctcaggcc tcgctccagg gcttgagctg
     1141 ccccgcttcg agcaccttga cctgctgctc caccgcttcg agccgaccgc ggcagaagct
     1201 cagcagttcg gcaccgcgct tgtagctttc cagcatctgg tccaggggca gctggccgcc
     1261 ttccatggcg gcgaccagac gctccagttc agccaaggcg tcttcatagc tcgcaggagc
     1321 gtcggacgcg acagctgata cggggttctt gggcatttct tatcgcgaaa gttggattgt
     1381 agtcacccgg aacctgcgcc aagcttgcgc gctcaccccc tgaaggggga tgccgcccgg
     1441 cccttcggcc gagtttgtgc agcctcaaga tgctgccggc ccccgcgggt agaatccgcg
     1501 gctcgccttt ggcgccccaa tcccacgctt cgtgccccga acgcaggctc gcagggtggg
     1561 ggtttgacat cggtttttgg agatcctggg gatcatgtcc gacctgagca tcacgctgga
     1621 agccctcgaa cgtagccaga cccaactggg cgttacgagt tatttcgata ccgatctgta
     1681 ccagcgggag ctcgaactca tcttccagcc aggtccgcgc tatctggggc acgagctgtg
     1741 cgtgcccgag atcggcgact accacaccct gcggcaagaa ggcgaaggcc gcaccctggt
     1801 gcgcacgccc gagggcctgc agctcatctc caacgtctgc cgccatcgcc aggccgtaat
     1861 gctgaacgga cggggcaaca cgcgcagcaa cgtcgtctgc ccgctgcacc gctggaccta
     1921 cgacctcaag ggccagctgg tcggcgcgcc gcatttcgcc gacgacccct gcctgcacct
     1981 caacaactac ccgctgcaga cctggaacgg gctggtgttc gagggccccg ccaaaggcgg
     2041 tcaagcggcc gcgcgcaacg tcgcggccga tctggccggc ctgggccagc tggccgactt
     2101 cgacttcagc ggctatgtgt tcgaccgcgt ccacttgcac cactgcgact acaactggaa
     2161 gaccttcatc gaggtctacc tggaggacta tcacgtcggc cccttccatc ccggcctcgg
     2221 caacttcgtt acctgcgagg acctgcgctg ggagatgggg cgcgagtact cggtgcagac
     2281 cgtgggcgtg gccaagtcgg gcgggcaggc gctgggcaag ccgggttcgg cggtctacaa
     2341 gcagtggcac gacgtgctgc tcgatttccg ccgcagccgc cacggcgagg aaccgcccaa
     2401 gcatggcgcg atctggctca cctactaccc gaccgtgatg ctggagtggt acccacacgt
     2461 gctggtggtg tccacgctgt atccgcaggg cccgcaaaag acgctgaatc tggtcgagtt
     2521 cttctacccc gaggagatcc aggccttcga gcgcgaattc gtcgaggccc agcaggccgc
     2581 ctacatggaa acctgcgtcg aggacgacga gatcgcattg cgcatggacg ccggccgcaa
     2641 ggccttgatg gagcgcggcg acaacgagat cggcccctac cagagcccga tggaagacgg
     2701 catgcagcac ttccacgagt ggtaccggcg cgcgatgcga ttgcaggcct gaagggccat
     2761 gtgccaagcc aagggccgct gcgcgcggcc cttgcttttt tcgggtcgcc ctcgtgcagg
     2821 cgatggccgc cgagccttac catcgcagcc atgtccgcac cgctgctgat gatcctcgcc
     2881 tccttcctgt tcgccaccat gggggtgtgc gtcaagctcg catcggcccg ctactcggcc
     2941 ggcgagatcg tgatgtaccg cggcctggtc ggcgcggcgc tgatgctggt gctgagccgc
     3001 ctgcgcggcg gcagtctcag gacggccgtg ccgacgatgc acttctggcg cagcctcacg
     3061 ggggtgtgct cgctgtgcct gtggttctac gccatcggca atctgccgct agccaccgcg
     3121 atgacgctga actacatgtc ctcggtgtgg atcgcgctgt tcctagtcgg gggcgccatc
     3181 atgctgggcg gcacgaaggt cgacggccgg ctcatcggca tcgtgctgct gggcttcatc
     3241 ggcgtgacgc tgatcctgcg cccgaccatg gagcacgacc agctctggca cggcctgatg
     3301 ggcctgctgt cgggcatgat ggcctccacc gcctacatgc aggtggcggc cttgggccgg
     3361 gccggcgagc ccgagtaccg cgtggtcttc tatttttcgc tcggcggggc cgtggcgggc
     3421 gggctggtcg ccgtcctgtt tggaggcggg ctgcatggtc acagcttaag cggcttcgcg
     3481 ctgctgatgg ccgtgggcct gctcgcaacc gcagcccagc tgatgatcac gcgggcctat
     3541 acggtgggca agctgctggt caatgcgagc ctgcaatacc tgggcattgc cttctccttc
     3601 ctctatggc
//
LOCUS       DASUCY010000358         3871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1003822, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000358 DASUCY010000000
VERSION     DASUCY010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1003822"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VFV25_07115"
     CDS             complement(<1..326)
                     /locus_tag="VFV25_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY1393129.1"
                     /translation="MRAGYLYGLAGVLLFALTIPMTRLAGGSVENPQLPPDFVAIGRA
                     AVAGLLALVWLAATRAPLPRRGFRGLLALSAAGAVFGFPLFLGWAVRHVEAVHAAVIT
                     GFLPLAT"
     gene            546..1967
                     /locus_tag="VFV25_07120"
     CDS             546..1967
                     /locus_tag="VFV25_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY1393130.1"
                     /translation="MPSLAAAPLARDADATLTAQLAARYAERIRQRLLAPGARLPSVR
                     ECAKRHGISTYTVVAAYDQLLAQGLIEARKQRGFFVRETQPPTRSRAAANEDRPPAPV
                     DAAALIRGMFQQPGSPLMPGFGTLPPDWLDLSMLSTALRRVTAGERLGPLALRYGEPA
                     GDSRLRAALAIKLDGFGLKVEASQIVTTIGATQALDIVSRTLLQPGDAVLVDEPGWAV
                     EFARLGRLGQRIVPVPRGADGPDLATMRSLIAAHKPRLYATVSVLHNPTGAMLSLAAA
                     HQVLKLAEVHDFHIVEDDTYAHLAPAHAPRLSALDGLQRTVYVSGFSKILTPAWRVGF
                     IAAPPALVERCIDVKLLSTLTTPAVLEQAVALCLEQGQLRRHAERVLQKLDAARARSV
                     QLALQAGMRFVSQPQGLFGWVDAGVDTDRLAALLLDEGWVLAPGSLFHATPRPTTLMR
                     INFATSQDARMWRALERAKNQLV"
     gene            2115..2894
                     /locus_tag="VFV25_07125"
     CDS             2115..2894
                     /locus_tag="VFV25_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393131.1"
                     /translation="MDEPTIPFGEPELWRVSAFRHAMGELSSRPDSADLGARLSLLSP
                     SLMADLQRFEHAPNGAEVLEVMAACLRHSQNVAIHLERTGYVLPITVFPRQRIFHSPL
                     PLSSLLDASLAELKLLQVEPAVLRAPGDKHRELVDVEHLYHPLGGLLWVLALYGPRKE
                     LLPEIAGPAAYRVAPGMDLRGLDIGGALASAIDRLRGEAVPLRVIAEWPGLDLERAAR
                     LLNGLYLQSGLMVSRTHPTALSLGARADGWLQSLTRRRSDN"
     gene            complement(2924..3175)
                     /locus_tag="VFV25_07130"
     CDS             complement(2924..3175)
                     /locus_tag="VFV25_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEY1393132.1"
                     /translation="MHYLWMFIVGIVVGLIARFIMPGSEHMGLIMTGVLGIAGSFVGG
                     FIARIFNKPADGALVHPAGLILSVVGALILLFAWSHLAK"
     gene            complement(3346..3702)
                     /locus_tag="VFV25_07135"
     CDS             complement(3346..3702)
                     /locus_tag="VFV25_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393133.1"
                     /translation="MKVRTARRLAALALVGIWSLFGFLFWAQAKAAQAQADTASPPAP
                     PVPPVLRSGARMTWAAPLPQDQPRTPICVQVIRPTTLVSGRPGALVRPEHGSAVVPEG
                     AVYEVYTGDLSDGCAD"
BASE COUNT          631 a         1385 c         1309 g          546 t
ORIGIN      
        1 gtggccagcg gcaggaagcc ggtgatgacg gccgcatgca ccgcctcgac atggcgcacg
       61 gcccaaccga ggaagagcgg gaagccgaac acggcgccgg ccgcgctcaa ggccagcagg
      121 ccccggaacc cgcggcgcgg caacggcgcg cgcgtggcgg cgagccagac cagcgccaac
      181 agccccgcga cggcggcgcg gccgatggca acgaaatcgg gcggcagctg cgggttctcg
      241 acactgccgc cggccaggcg cgtcatcggg atcgtcagcg cgaacagcag cacgccggcc
      301 agcccgtaga gatagcccgc acgcaggcga cgctgctccg cgcttgagcc ggcgctctgc
      361 gcggggtcat gcgctaccaa tggcgggcgg gaagcggtcc gatacatgag tcaaaaaggg
      421 gattgggctc gaccagtagc gagcatgaac cagtgtatgg acttgaccaa cacagaacca
      481 atacactgcg cctgttcagt cttcgtgctg tatcggtcac cgctccaaca catcgcatcc
      541 ccgccatgcc cagcctcgcc gcagccccgc tggcgcgaga cgccgacgcc acgctcaccg
      601 cgcagctcgc cgcgcgctac gccgaacgca tacgccagcg cctgctggcg ccgggcgcgc
      661 gcctgccctc ggtgcgcgaa tgcgccaagc gccacggcat cagcacctat acggtggtcg
      721 cggcctacga ccagttgctg gcccaaggct tgatagaggc gcgcaagcag cgcggcttct
      781 tcgtgcgcga gacccagccg ccaacgcgca gccgcgccgc agcgaacgag gatcgcccgc
      841 ccgcgccggt cgacgccgcg gcgctgatac gcggcatgtt ccagcagccc ggcagcccgc
      901 tcatgcccgg cttcggcacg ctgccgccgg attggctgga cctgtccatg ctcagcacgg
      961 cgctgcgccg cgtgacggcg ggcgagcgcc tgggcccgct ggcgctgcgc tacggcgagc
     1021 cggccggcga ctcgcggctg cgcgcggccc tggcaatcaa gctggacggc ttcggcctga
     1081 aggtggaggc ctcgcagatc gtgacgacga taggcgccac ccaggcgctg gacatcgtct
     1141 cgcgcaccct gctgcagccg ggcgacgcgg tgctggtcga cgagcccggc tgggcggtgg
     1201 aattcgcccg cctgggccgc ctgggtcagc gcatcgtgcc ggtgccgcgc ggcgccgacg
     1261 ggcccgacct ggcgacgatg cgctcgctca tcgccgcaca caagccgcgc ctttacgcca
     1321 ccgtctcggt gctgcacaac ccgaccggcg cgatgctctc gctggccgca gcgcaccagg
     1381 tgctgaagct ggccgaggtg cacgacttcc acatcgtcga ggacgacacc tatgcccacc
     1441 tggcgcccgc ccacgcgccg cggctatcgg cgctggacgg gctgcagcgc accgtctacg
     1501 tctcgggctt ttccaagatc ctcacgccgg cctggcgcgt gggcttcatc gccgcgccgc
     1561 cggccctggt ggagcgctgc atcgatgtca agctgctgtc cacgctgacc acgccggcag
     1621 tgctggagca ggccgttgcg ctgtgcctgg agcagggcca gctgcgccgc cacgccgaac
     1681 gcgtgctgca gaagctcgac gctgcgcgcg cgcgcagcgt gcagctggcg ctgcaggccg
     1741 gcatgcgctt cgtgagccag ccccagggcc tgttcggctg ggtcgatgcg ggcgtggaca
     1801 ccgaccgcct ggccgcactg ctgctggacg agggctgggt attggcgccg ggctcgctgt
     1861 tccacgccac gccacggccg accacgctga tgcgcatcaa cttcgcgact tcgcaggacg
     1921 cgcgcatgtg gcgcgcgctg gaacgcgcca agaatcagct cgtctgaaac aaagcgaacg
     1981 gaggttttga gggaaaacca ccgactcttc gaggcatatt tgtcttgcgg cgcacccaaa
     2041 tatgtagcga gtcgggttgg gggtacagcg acccgagggt ggcggcacgc cggtagcgca
     2101 ctaaagtcgc acccatggac gagcctacca tccccttcgg cgagccggag ctgtggcgcg
     2161 tcagcgcctt ccggcacgcg atgggcgagc tttcaagcag gcccgattcg gccgacctgg
     2221 gcgccagact gtcgctgctc agcccctccc tgatggccga cctgcagcgc ttcgagcatg
     2281 cacccaacgg ggccgaggtg ctggaggtca tggcggcctg cctgcgccac tcgcagaacg
     2341 tcgcgatcca cctcgagcgt accggctatg tgttgccgat cacggtcttc ccgcgccagc
     2401 gcatttttca ttcgccgctg ccgctgagct cgctgctgga tgcgagcctc gccgagctaa
     2461 agctgctgca ggtcgagccg gctgtgctgc gcgcgcccgg cgacaagcac cgcgagctgg
     2521 tcgacgtcga gcatctctac cacccgctgg gcggcctgct gtgggtgctg gccctgtacg
     2581 gcccccgcaa ggaattgctg cccgaaatcg ccgggccggc ggcctaccga gtcgcccctg
     2641 gcatggatct gcgcggcctg gatatcggcg gtgcgcttgc gtccgccatc gatcgcctgc
     2701 gcggcgaagc agtgccgctg cgcgtcatcg ccgaatggcc ggggctggac ctggaacgag
     2761 ccgcgcggct gctcaatggc ctgtatctgc agtccggcct gatggtaagc cgcacccatc
     2821 cgacggcgct aagcctgggc gcgcgcgccg acggctggtt gcaatcgctg acgcggcgcc
     2881 gctcggacaa ctgaggccgc cgcgcagcgc caccggttcg cgcctacttc gccaagtggc
     2941 tccaggcgaa cagcaggatc aatgcgccaa cgaccgagag gatcagcccg gcgggatgca
     3001 ccagcgcgcc gtccgccggt ttgttgaaga tgcgcgcgat gaagccgccg acgaaggagc
     3061 cggcgatgcc cagcacgccc gtcatgatga ggcccatgtg ctcggacccc ggcatgatga
     3121 aacgtgcgat caggccgacc acgataccga cgatgaacat ccacagatag tgcagcacgg
     3181 tctctcctca gctttggtat tgatgctcga agagccggaa gcctcaggcc ccggcaggat
     3241 ccgccaggcg gtagcccgac gccgcgcgat attaccggcg catcgcgccg gccgacaccc
     3301 aggcggcacc ctagccggtc cgcaagggag tcgtcagctt agggcttagt cggcacagcc
     3361 gtcgctcaga tcgccggtat acacctcgta gaccgcccct tccggcacga ccgcggaacc
     3421 gtgctcgggt cgcaccaggg cacccggacg gcccgagacc agggtggtgg gccggatcac
     3481 ctgcacgcag atcggcgtgc gcggctggtc ttggggcaaa ggcgcggccc aggtcatgcg
     3541 ggcccccgat cgcaagaccg gcgggaccgg cggcgccgga ggcgaagcgg tatccgcctg
     3601 agcctgagca gccttggcct gcgcccagaa caagaagccg aagagactcc aaatgccgac
     3661 gagagcgagc gccgcaaggc ggcgcgccgt gcgtactttc attttgtttt cctccctctg
     3721 ccactcacgc gaagcggcag cgcggctgat tatcgcttcg ccaccggcag gggatccagc
     3781 gagagccctt gctccttatc gtcagccaag cgaccgcggc tcgtagcgca gccctatcgc
     3841 cacccgatcg gtccaccaga cgaggcggcc g
//
LOCUS       DASUCY010000359         2300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1051906, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000359 DASUCY010000000
VERSION     DASUCY010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051906"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VFV25_07140"
     CDS             <1..472
                     /locus_tag="VFV25_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393134.1"
                     /translation="FWRNRRNERLRAGSTLPQGWVRPATGVPMLSDDALGLHWALVGV
                     GIDPTTRLRPDQLHSWQRAGGKVWQWCQRAQAQHLAAADQRLEALDETLLPRRTPLGW
                     VVIVRPDRCVMAEGPVEQVQELLDQALRRLAPEPEHLPKRSANLGAWCHRCDVN"
     gene            457..1482
                     /locus_tag="VFV25_07145"
     CDS             457..1482
                     /locus_tag="VFV25_07145"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEY1393135.1"
                     /translation="MRRELICLEPSSPPRLQVRESAIPEPQRGKVLVRVEATSVNPID
                     AKRAGGYGQRLLAFKGAGRFPLVLGNDVAGRVEAVGAGVSTLAPGQRVFGLVATGKGG
                     GAHASHVVVPQSLLQAAPQEAEAPALAVLPYSFTTMWLAVRSTRLTHANAAGKRVLVH
                     GAAGALGRLALQWLSGWGCHITAICDTGKSDDCRALGARVAVERGPQAIRSLPTDFDV
                     VLNFASWDDDLALASRLGGNGLGHATTVHPLLGHFDRLGWLRGALASQRDAHTVRSAV
                     HQRSAGAHYAWTIFKPDHEALAALATGIRERRLSLPVGLCAGFDQADRAFAHVAAGKP
                     GRAVLQP"
     gene            1516..>2300
                     /locus_tag="VFV25_07150"
     CDS             1516..>2300
                     /locus_tag="VFV25_07150"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY1393136.1"
                     /translation="MNGIAGMSDVETIERNRPTLPNSTYEMIGQAAAAHADAPALSFF
                     LRAQDHDRPLTWDYATLFSQITATANFFHGLGIDKDDVIAFVLPNLPETHLTIWGGQA
                     AGIVFAINPLLEPAAIVDLLKAGGAKVLVTLAPFPGSDLWPKLQPVIAEVPGLRHMVL
                     VDLAEHLPGLPALPTALPQGVEIGTDRIAVHAFAHGLRGQPTDRLVSGRVIEPDDFSS
                     FFCTGGTTGTPKIAMRRHASEVANAWSVGQVLGDGMAAGKTLF"
BASE COUNT          348 a          818 c          785 g          349 t
ORIGIN      
        1 gttctggcgc aaccggagga acgagcggct gcgtgcgggc tcgacgctac cgcagggttg
       61 ggtgcgaccg gccacaggcg tccccatgct gagcgatgac gcgctgggcc tgcattgggc
      121 gctcgtgggc gtcggcatcg accccacgac gcgactgcgc cccgaccagc tgcacagctg
      181 gcagcgcgcc ggcggcaagg tctggcagtg gtgccagcgc gcacaggcac agcacctggc
      241 agccgcggac cagcgcctgg aggcattgga tgaaacgctg ctgccgcggc gaactcccct
      301 gggctgggtc gtcatcgtcc gcccggaccg ttgcgtgatg gccgagggac cggtcgagca
      361 ggtgcaagag ctgctcgacc aggcacttcg ccgtctggcg cccgaacccg aacatctgcc
      421 gaagcgaagt gccaacctgg gtgcttggtg ccaccgatgc gacgtgaact gatctgcctg
      481 gagccgtcat cgcccccgcg actccaagtg cgcgagagcg ccatacccga gccgcaacgc
      541 ggcaaggtgc tcgtgcgggt cgaagcgacg tccgtgaatc cgatcgatgc caagcgtgcc
      601 ggcggctacg ggcagcgcct gctcgcattc aagggcgcgg ggcgttttcc gctggtgctc
      661 ggcaacgacg tggcaggccg cgtcgaagcg gtcggcgcgg gcgtctcgac gctggccccg
      721 ggacagcggg tgtttgggct agtggccacc ggcaagggcg gcggcgccca cgcttcgcat
      781 gtcgtggttc cgcagtcgct gctgcaggcc gctccgcagg aggccgaggc tccggccctc
      841 gccgtgctgc cctactcgtt cacgacaatg tggctggcgg tgcgttcgac gcggctcacc
      901 cacgcgaacg cggcaggcaa gcgcgtcctc gtccacggcg ccgccggggc gctggggcgg
      961 ctagccctgc aatggctgtc ggggtgggga tgccacatca cggcgatctg tgacaccggc
     1021 aagtccgacg actgccgcgc actcggcgcg cgagtcgccg tcgagcgggg gccgcaggcc
     1081 atccgttccc tgccgaccga cttcgacgtt gtgctgaact tcgccagctg ggacgacgac
     1141 ctcgcactcg cgtcacgcct gggcggcaac ggactcggcc acgcgacgac cgtgcatccg
     1201 ctgctgggcc acttcgaccg gctgggctgg ctgcgcggag cgctcgccag ccagcgtgat
     1261 gcccacaccg tgcgttcggc cgttcatcag cggtccgccg gagcacacta cgcctggacg
     1321 atcttcaagc cggaccacga agcactcgcc gctctggcca ccggcatccg cgagcgccgg
     1381 ctctcgttgc cggtgggcct gtgcgccggc ttcgaccagg cagaccgagc gttcgcccac
     1441 gtcgcagcag gaaagcccgg ccgggccgtg ctgcaaccct gacgtcatcg cccgacccga
     1501 acgaggatga accccatgaa tggcattgcc ggcatgtccg atgtcgagac catcgagcgc
     1561 aatcgcccga cgctgcccaa cagcacttac gagatgatcg gccaggcggc cgcggcccac
     1621 gccgacgcgc cggcgctgtc gttcttcctg cgcgcgcaag accacgaccg gccgctgacc
     1681 tgggattacg cgacgctgtt ttcccagatc acggcgaccg ccaacttctt ccacggcctg
     1741 ggcatcgaca aggacgacgt catcgcgttc gtgttgccca acctgccgga gacccatctc
     1801 acgatctggg gcggccaggc cgccgggatc gtcttcgcga tcaacccgct gctggagccg
     1861 gccgcgatcg tcgatctgct gaaggccggt ggcgcgaagg tgctggtcac gctcgcgccc
     1921 ttccccggct ctgacctctg gccgaaactg cagcccgtga tcgccgaagt gcccggcctg
     1981 cggcacatgg tgctggtcga ccttgccgag catttgcctg gtctgcccgc gctgcccacc
     2041 gcgctgccgc aaggtgtcga gatcggcacc gaccgcatcg cggtgcatgc ctttgcccac
     2101 ggcctgcgcg gtcaaccgac cgacaggctg gtcagcggcc gtgtgatcga accagacgac
     2161 ttctcttcct tcttctgcac cggcggcacg acgggaaccc cgaagatcgc gatgcgccgc
     2221 cacgcgagcg aggtagccaa tgcgtggagc gtcggccagg tgctgggcga cggcatggcg
     2281 gccggcaaga cgctcttctg
//
LOCUS       DASUCY010000360         3272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_216585, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000360 DASUCY010000000
VERSION     DASUCY010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216585"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="VFV25_07155"
     CDS             complement(<1..701)
                     /locus_tag="VFV25_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349037.1"
                     /note="catalyze the formation of cyanophycin which may act
                     to store excess nitrogen; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEY1393137.1"
                     /translation="MEVSRIRALRGPNLWSRHTAIEAIVTCPARERSLTLMPGFEARI
                     RGLFPGLGVLQTDGQPAQVSLAHVLEAATLALQKEAGCPVTFSRTTATLEPGVYQVVV
                     EYSEEDVGRQALELAEALVQAALKETGFDVEAAVARLREVDEDVRLGPSTGSIVTAAA
                     ARGIPWRRLTQGSLVQFGWGSRQRRIWAAEVDTSSAVSESIAQDKDLAKRLLHAAGVP
                     VPIGRPVSSVEDAWAV"
     gene            complement(724..2898)
                     /gene="cphA"
                     /locus_tag="VFV25_07160"
     CDS             complement(724..2898)
                     /gene="cphA"
                     /locus_tag="VFV25_07160"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEY1393138.1"
                     /translation="MSTKDDIKLLRINYLRGPNIWTYRPVLEVWLDLGALEDHPSHQL
                     PGFNARLLALLPALEEHHCGVGERGGFLQRLHEGTWAGHVLEHVVIELLNLAGMPTGF
                     GQTRSTSQHGVYRMVFRARDEQVARTALAAGHRLLMAAVNDESFDVAAAVAQVREQVD
                     ESYFGPSTAAIVAAATERRIPHIRLNDGNLVQLGYGASQHRIWTAETDLTSAIAEGIA
                     RDKDLTKGLLKSCGVPVPEGEIVASPAAAWEAAQGIGLPVVIKPTDGNHGRGVTLDLD
                     TREDIEAAYAFAERHGSEVIVERYVRGQEHRLLVVGGKLVAAARGETAWVTGDGRSTV
                     TELVNAQINTDPRRGDTEDFPLARIKADEDGAVLSDLQRQSLTPASVPAAGRQVLIQR
                     NGNVAIDCTDQVHPEVEHVVSLAARVVGLDIAGVDVVAEDISRPMQEQGGAIVEVNAG
                     PGLLMHLKPAEGTPRPVGRAIVDHLFAEDNDGRIPIVGVAGSRDTAVIARLVAWLVQL
                     SGRPVGLACRDGLFLSGRQVERRDCANWEAGHRLLMNREVQAAVFENGAESILRDGLA
                     YDRCRVGIVTDLEGAEGLDHYDIRESDQLVKVLRTQVDVVLSDGAAVLNAADPRVADL
                     AELSDGEVIFYAQDAQCEALAAHRAQGGRAVFAKDGRVILASGVGETPCATLSGGSKR
                     RRKEIAPEALLAAVAAAWGLGIAPELIAAGIETYAPDASQPR"
     gene            3057..>3272
                     /locus_tag="VFV25_07165"
     CDS             3057..>3272
                     /locus_tag="VFV25_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393139.1"
                     /translation="MQQAHLLPVDPGPVERLRSALNGQLGTNENVLGTLEVDLDGDLR
                     FAPGLLTLTDQRLLACEPGGASWRSWPL"
BASE COUNT          450 a         1185 c         1142 g          495 t
ORIGIN      
        1 acggcccagg catcctcgac gctgctcacc ggccggccga tgggcaccgg cacgccggcc
       61 gcgtgcagca ggcgcttggc caggtccttg tcctgcgcga tcgactcgga cacggcgctg
      121 ctggtgtcga cctcggcggc ccagatgcgg cgctgcctgg agccccagcc gaactgcacc
      181 aggctgccct gggtcaggcg ccgccagggg atgccgcgtg cggccgcggc ggtcacgatg
      241 gagccggtgc tggggcccag gcgcacgtcc tcgtcgacct cacgcagacg cgccacggcg
      301 gcctcgacgt cgaagccggt ttccttgagg gcggcctgca ccagggcctc ggccagttcc
      361 agcgcctggc ggcccacgtc ttcctcgctg tactcgacca ccacctggta gacgcccggc
      421 tctagcgtcg ccgtggtgcg gctgaaggtc accgggcaac cggcctcctt ctgcagcgcc
      481 agggtcgccg cctcgagcac atgggcgagc gagacctggg ccggctggcc gtcggtctgc
      541 agcacgccca ggcccggaaa gagcccgcgg atgcgcgcct cgaagcccgg catcagcgtg
      601 agcgaacgct cgcgcgccgg gcaggtgacg atggcctcga tggccgtgtg acggctccag
      661 aggttggggc cgcgcagggc ccggatgcgg gaaacttcca tcggcttatt cgtctctagc
      721 aaatcatcgc ggctgggacg cgtcgggggc ataggtctcg ataccggcag cgatcagctc
      781 gggcgcgatg cccaagcccc aggccgcggc cacggcggcc agcaaagcct ctggggcgat
      841 ctccttgcgg cggcgcttgc tgccgccgct caaggtcgcg cagggcgtct cgcccacgcc
      901 gctggccagt atcacgcggc cgtctttggc aaacacggcg cggccgccct gagcgcgatg
      961 cgcggccagg gcttcgcatt gagcatcctg ggcgtagaag atcacctcgc cgtcgctcag
     1021 ctcggccaga tcggcgacgc gcgggtcggc ggcgttcagt accgccgcgc catcggacag
     1081 caccacatcg acctgggtgc gcagcacctt cacgagctga tccgactcgc ggatgtcata
     1141 gtgatccagg ccctcggcgc cttccagatc ggtgacgatg ccgacgcggc agcggtcgta
     1201 ggccaggccg tcgcgcagga tggattcggc gccgttctcg aacaccgcgg cctgcacctc
     1261 gcggttcatc agcaggcggt ggccggcctc ccagttcgcg cagtcgcggc gctcgacttg
     1321 ccggccggag aggaagaggc cgtcgcggca ggccaggccc accgggcggc cgctgagctg
     1381 caccagccag gccaccagcc gcgcgatgac ggccgtgtcc cgcgagccgg cgacgccgac
     1441 gatggggatg cgcccgtcgt tgtcctcggc aaacagatgg tcgacgatgg cgcgccccac
     1501 cggccgcggc gtgccttcgg ccggcttcag gtgcatcagc aggccaggcc cggcattgac
     1561 ctcgacgatc gccccgccct gctcctgcat cgggcgcgag atgtcctcgg ccacgacgtc
     1621 gacgccggcg atgtccagcc cgaccacgcg ggcggcgagc gagaccacat gttcgacctc
     1681 gggatggacc tggtcggtgc agtcgatggc cacgttgccg ttgcgctgga tcagcacctg
     1741 ccgcccggcc gccggcaccg aggccggagt gaggctctgg cgctgcaggt cggacagcac
     1801 ggcgccgtcc tcgtcggcct tgatgcgcgc cagcggaaag tcctcggtat cgccgcgccg
     1861 cggatcggtg ttgatctggg cgttgaccag ctcggtcacg gtggagcggc cgtcgcccgt
     1921 gacccaggcc gtctcgccgc gcgccgcggc gaccaacttg ccgcccacca ccagcaggcg
     1981 gtgctcctgg ccgcgcacat agcgctccac gatgacctcg ctgccatggc gctcggcgaa
     2041 ggcgtaggcc gcctcgatgt cctcgcgcgt gtcgaggtcc agggtcacgc cgcgcccgtg
     2101 gttgccgtcg gtgggcttga tcaccaccgg caggcctatg ccctgcgccg cctcccaggc
     2161 cgcggccggg ctggccacga tctcgccttc gggcaccggc acgccgcagg acttgagcag
     2221 gcccttggtc aggtccttgt cgcgtgcgat gccctcggcg atcgcgctgg tgagatcggt
     2281 ttcggcggtc cagatgcggt gctggctggc gccgtagccc agctgcacga ggttgccgtc
     2341 gttgagccgg atgtgcggga tgcggcgctc cgtggccgcc gcgacgatgg ccgccgtgct
     2401 gggcccgaaa taggattcgt cgacctgctc gcgcacctgc gccaccgccg ccgcgacgtc
     2461 gaagctttcg tcgttgacgg ccgccatcag cagcctgtgg ccggcggcga gcgcggtgcg
     2521 tgccacctgc tcgtcacgcg cacggaacac catgcggtag acgccatgct gcgaggtgct
     2581 gcgcgtctgg ccgaagccgg tgggcatgcc cgcgagattc agcagctcga tcaccacatg
     2641 ctccagcacg tggccggccc aggtgccttc gtgcagccgc tgcaggaagc cgccgcgctc
     2701 gccgacgccg cagtgatgct cttccagcgc cggcagcagc gcgagcagcc gcgcgttgaa
     2761 gccaggcaac tgatgcgagg ggtggtcttc gagcgcgccc aggtccagcc agacttcgag
     2821 cacgggacgg taggtccaga tattggggcc gcgcagatag ttgatgcgca gcagcttgat
     2881 gtcgtccttc gtgctcatga tgtcctgcgg gctccatgcc ccgcgccagt agtggcgccg
     2941 cccaagattg tgcgcccgcc gcggcctgcc atcggcctgg gaaagctgcc gctgccggct
     3001 tgggcgaaaa tccccgacct tgcccgggaa tcgcgcccgc ggcccctccc atcacgatgc
     3061 aacaagctca tctgctccca gtcgatcccg gccctgtcga gcgcctgcgc tcggccctga
     3121 acgggcagct cggaaccaac gaaaacgtgc tgggcacgct ggaggttgac ctggacgggg
     3181 atcttcgctt cgcgcccggc ctgctgaccc tgaccgacca gcgcttgctg gcctgcgagc
     3241 cgggcggcgc gtcctggcgc tcttggccgc tc
//
LOCUS       DASUCY010000361         2237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1084135, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000361 DASUCY010000000
VERSION     DASUCY010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084135"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..610
                     /locus_tag="VFV25_07170"
     CDS             62..610
                     /locus_tag="VFV25_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HEY1393140.1"
                     /translation="MKFIHPNQDAWRTLGGEDGPHVTITPEPRRLLTLAQWHAVRSQW
                     PADLPVAVSVANEDDIEVLEADLPRIAMVALSFPKWVDGRAYSQARLLRSRYRYAGEV
                     RATGEVLVDMLPLLARTGFDAVVLKPGQSQEAAERALAFFRGHYQGDTHDNRPLFAMP
                     AGTGEALAREPKQEFVNAGAAI"
     gene            607..1359
                     /locus_tag="VFV25_07175"
     CDS             607..1359
                     /locus_tag="VFV25_07175"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEY1393141.1"
                     /translation="MSAIELYARRSADHEAKIERAVALLREAAARYAGKIVQSTSLGV
                     EDMVVTDLIARHGIPVALGTLETGMLHPQTVELIGRIETHYPGLKVEVYRPTQESVVH
                     FVKTNGDQAMYQSIELRKACCRIRKLEPLARMLADRSAWVTGLRREQSNDRGGVPFRD
                     HDDQGREKINPLADWTWGDVWAYVSEHGVPYNPLHDEFMPSIGCAPCTRAIAVGEDFR
                     AGRWWWEQEGQKECGLHVHQDEPVVSMIGGAR"
     gene            1356..>2237
                     /gene="cysD"
                     /locus_tag="VFV25_07180"
     CDS             1356..>2237
                     /gene="cysD"
                     /locus_tag="VFV25_07180"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009336 - sulfate adenylyltransferase
                     complex (ATP) [Evidence IEA];
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA];
                     GO_process: GO:0070814 - hydrogen sulfide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEY1393142.1"
                     /translation="MNAPLNVDQLLPELDQRHLDALEEEAIFILREVAAAFERPALLF
                     SGGKDSCVVLRLAEKAFKMRRQGNDFDGRLPFPLLHVDTGHNFPEVIEFRDRRVAEMG
                     ERLVVGHLEDSIKRGSIRLAHPLESRNGHQTVTLLEAIEEHRFDVLIGGARRDEEKAR
                     AKERIFSHRDSFGQWQPKEQRPELWTLFNTRLKPGEHMRAFPISNWTELDVWLYIARE
                     NIPLPNLYYAHDRQVIRRKGLLVPLTEVTPPDAGEQVETAQVRFRTVGDMTCTCPVES
                     PAATPAEIVGETLTVTIS"
BASE COUNT          397 a          776 c          742 g          322 t
ORIGIN      
        1 gctgccaacg ccgtgcgccg cagcaccgcg acggatcgcc acgccgcttg aggactcgca
       61 catgaagttc atccatccca atcaggacgc ctggcgcacg ctgggcggcg aagacggccc
      121 gcacgtcacg atcacgcccg aaccgcgccg gctgctcacg ctggcgcaat ggcacgcggt
      181 gcgcagccaa tggccggccg acctgccggt ggcggtgagc gtcgcgaacg aagacgacat
      241 cgaggtgctg gaagcagacc tgccgcgcat tgcgatggtg gcgctgagct ttccgaagtg
      301 ggtcgacggc cgcgcctaca gccaggctcg gctgctgcgc tcgcgctacc gctatgccgg
      361 ggaagtgcgc gccaccggcg aggtgctggt cgacatgctg ccgctgctgg cccgcaccgg
      421 cttcgacgcc gtcgtgctga agccgggcca atcgcaggaa gccgccgaac gggcgctggc
      481 cttcttccgc ggccattacc agggcgacac gcacgacaac cggccgctgt tcgcgatgcc
      541 ggccggtact ggcgaggctt tggcacgcga gcccaagcag gaattcgtga acgctggagc
      601 ggcaatatga gcgccatcga gctgtacgcc cgacgcagcg ccgatcacga agccaagatc
      661 gaacgcgccg tcgctctgct gcgcgaggct gcagccaggt acgccggcaa gatcgtgcag
      721 tccaccagcc tcggcgtcga ggacatggtg gtcaccgacc tgatcgcccg ccacggcatt
      781 cctgtcgcgc tgggcacgct ggaaaccggc atgctgcacc cgcagacggt ggagctcatc
      841 gggcgcatcg agacgcatta ccctggcctc aaggtcgagg tctacaggcc gacgcaggag
      901 tcggtggtcc atttcgtcaa gacgaacggc gaccaggcca tgtaccagag catcgagctg
      961 cgcaaggcct gctgccgtat ccgcaagctg gagccgctgg cgcgcatgct ggccgatcgc
     1021 agcgcctggg tcaccggcct gcgccgcgag caatccaacg atcgcggcgg cgtgcccttc
     1081 cgcgaccacg acgaccaggg ccgcgagaag atcaatccgc tggccgactg gacctggggc
     1141 gacgtgtggg cctatgtttc cgagcatggc gtgccctaca accccctgca cgacgaattc
     1201 atgcccagca taggctgcgc gccctgcacg cgcgccatcg ccgtgggcga ggatttccgc
     1261 gccgggcgct ggtggtggga acaggaaggc cagaaggaat gcgggctgca tgtgcatcag
     1321 gacgagcccg tcgtatcgat gattggagga gcgcgatgaa cgcccccttg aatgtggatc
     1381 aactgctgcc ggagctcgat cagcggcatc tcgatgcgct ggaggaagaa gccatcttca
     1441 tcctgcgcga agtcgccgcc gccttcgagc gcccggccct gctgttctcc ggcggcaagg
     1501 actcctgcgt ggtgctgcgc ctggccgaga aggccttcaa gatgcgccgg cagggcaatg
     1561 atttcgacgg ccgcctgccc ttcccgctgc tgcacgtgga caccgggcac aacttccccg
     1621 aggtcatcga gttccgcgac cgccgcgtgg ccgagatggg cgagcgcctc gtggttggcc
     1681 atctcgaaga ctccatcaag cgcggcagca tccgcctcgc gcacccgctc gagtcgcgta
     1741 acggccacca gaccgtgacg ctgctcgagg cgatcgagga gcaccgcttc gacgtgctga
     1801 tcggcggtgc gcggcgcgac gaggaaaagg cgcgcgccaa ggagcgcatc ttcagccacc
     1861 gcgacagctt cggccaatgg cagcccaagg agcagcgccc cgagctgtgg acgctcttca
     1921 acacgcgcct caagccgggc gagcacatgc gcgccttccc gatcagcaac tggaccgagc
     1981 tcgacgtgtg gctctacatc gcccgcgaga acatcccgct gccgaacctg tactacgcgc
     2041 acgaccgcca ggtgatccgc cgcaaaggcc tgctggtgcc gctcaccgag gtgacgccgc
     2101 ccgatgccgg cgagcaggtc gagaccgcgc aggtgcgctt ccgcaccgtg ggcgacatga
     2161 cctgcacctg cccggtggaa agccccgctg caacgccggc cgagatcgtc ggcgagacct
     2221 tgaccgtgac catcagc
//
LOCUS       DASUCY010000362         4989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1469506, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000362 DASUCY010000000
VERSION     DASUCY010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4989
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1469506"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /gene="queF"
                     /locus_tag="VFV25_07185"
     CDS             complement(<1..378)
                     /gene="queF"
                     /locus_tag="VFV25_07185"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEY1393143.1"
                     /translation="MPVNPPSAPSKELHVFPNPSPKRDYVIQFQVPEFTCHCPLTGQP
                     DFAHFTIDCIADQLCIELKSLKMYMWSYRNEGAFHEKVTNDILDDIVKAIAPRFLRIT
                     AKWYVRGGIYTNVVVEHRKKGWKP"
     gene            602..4117
                     /gene="smc"
                     /locus_tag="VFV25_07190"
     CDS             602..4117
                     /gene="smc"
                     /locus_tag="VFV25_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEY1393144.1"
                     /translation="MRLNSIKLSGFKSFAEPTNFQLPGQLVGVVGPNGCGKSNIMDAV
                     RWVLGESKASELRGESMQDVIFNGSGTRKPASRSSVELVFDNSSARAGGQWNQYAEIA
                     VKRVLTRDGTSSYYINNQPVRRRDVQDVFLGTGLGPRAYAIIGQGTISRIIESKPEEL
                     RLFLEEAAGVSKYKERRRETENRLKDTRENLTRVEDILRELNNNLAHLEKQAEVATQY
                     RGLQEQGTLKLHQLWFLKHRDAAGEEERVKKAVLEATNALESRLAELRHVEAELEQVR
                     QAHYRASDDLHAAQGTLAEAALEVSRLEERIRYVVEGRARVEARLAELKAQNEQWGER
                     RQQAQDELEHIAEQIAAAEEQAELLAAQAEEQSGALPNHEDAVRAAQSKSNEQRGSVA
                     QVQQQIQLLAAESRNIDEQGRALAQRRERLASERQGLAQPDGARMAELKQQLADAEES
                     QAMAEARLHELTEQVPALDEDRRARQEQVNAEAARQADLNARLEALRALQEKVQTEGK
                     LKPWLAKHGLDGLAGLWTKVHIETGWETALESALREKLNALEVGRVETVRAFENDAPP
                     AKLAFYTAPAGVIANTHQTLPRLSDLLRLGDAGLKALLNDWLEGVYTAGSLDEALASR
                     SKLTHGEVIMTKAGHAVSQFAVAFYAPDSEQAGMLARAQEIENLEKQTRAQSLLVEEA
                     RSALVRAEAAYTEASQGLVSARREAAEGQTRTHQLQVETLRLTQLAEQTSQRRGQIEG
                     ELAEIDAGLEELQERRITGEARFEELDMQLANEQERHAELDEAVIQAERRQGEAREQL
                     RSLERQAQEAQFSARSLAARRGELQRSIEQAVQMIAANEQSATQLHDELAQFNDQAAQ
                     AGLQEALALKLEREGALAAQRSQYDDLSAQLRRADEQRLEFERSLDPLRERITKLQLE
                     EQAAQLGGAQFMEQLTAAQVDLEALARSIEEGGVKLYGLQGEIDRINRDINSLGAVNL
                     AALDELAAARERKTFLDAQSADLVEAMNTLEGAIHKIDLETRDLLGSTFNTVNEHFGR
                     MFPSLFGGGQAKLVMTGDEILDAGVQVMAQPPGKKNSTIHLLSGGEKALTAIALVFAI
                     FQLNPAPFCLLDEVDAPLDDANTERYSKLVKSMANNTQFLFISHNKIAMEMAEQLIGV
                     TMQEQGVSRIVAVDMEAAVSMAEAA"
     gene            4149..>4989
                     /locus_tag="VFV25_07195"
     CDS             4149..>4989
                     /locus_tag="VFV25_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEY1393145.1"
                     /translation="MSSLQVSLAVLGGLVLAGIVAHSAWQARRAGVRTAPQSAARQEP
                     REPVLGDGATPPAAPQMAGETEPAATLGQERLDLPPLLRRSTARLDALIDAIASLTLE
                     APVSAETALLHLPPSRRAGSKPFMIEGLNTESGDWELPALGQRYGEFQAGVQLANRTG
                     ALNEIEYSEFVQKVQAFAEAVGAMADFPDMLDVVARARELDAFAGDHDAQLAVHLRSK
                     AVAWSVGYIQQHAGWHGFVAGVVPGRLVLPSAEEGAPPVLTLTFDSQAALADDPGRAA
                     VRDV"
BASE COUNT          900 a         1639 c         1742 g          708 t
ORIGIN      
        1 cggtttccag cccttcttgc ggtgctcgac caccacgttg gtatagatgc cgcctcgcac
       61 ataccacttg gcggtgatgc gcaggaagcg cggcgcgatg gccttcacga tgtcgtccag
      121 gatgtcgttc gtgaccttct cgtggaaagc gccttcgtta cggtagctcc acatgtacat
      181 cttgaggctc ttgagctcga tgcagagctg gtcggcgatg cagtcgatcg tgaaatgcgc
      241 gaagtcgggc tggccggtga gcgggcagtg gcaggtgaac tcgggcacct ggaactggat
      301 cacgtagtcg cgcttcgggc tcggattcgg gaagacgtgc agttccttcg aaggcgccga
      361 cggcggattc accggcatcg gacgggtctt gtgggccggt gctttggcga tgactttgct
      421 ggaggttttg gccatggctg gatgcgcgcc gccgcttgcg tgcggcgacg attgctgaca
      481 tttctggagg aggtgcgatg gtatcatccg acccgctcat ggccctgcgg aaccgcaggt
      541 tttttcttat aaatcaacga gttagcggcg cggccccggg gcgccggatg accgcgtacg
      601 catgcgcctg aactcgatca agctctccgg cttcaagtcg ttcgccgaac ccacgaattt
      661 ccagcttccc ggccagctcg tcggcgtggt cgggcccaac ggctgcggca agtccaacat
      721 catggacgcg gtgcgctggg tgctcggcga atccaaggcc tccgagctgc gcggcgagtc
      781 catgcaggac gtcatcttca acggctcggg cacgcgcaag ccggccagcc gctccagcgt
      841 cgagctggtg ttcgacaaca gcagcgcacg cgccggcggc cagtggaacc aatacgccga
      901 gatcgccgtc aagcgcgtgc tgacgcgcga cggcacctcg agctactaca tcaacaacca
      961 gccggtgcgc cgccgcgacg tgcaggacgt gttcctgggc accggcctcg gcccgcgcgc
     1021 ctacgccatc atcggccagg gcacgatcag ccgcatcatc gagtccaagc ccgaggagtt
     1081 gcgcctcttc ctcgaagagg ccgccggcgt ttccaaatac aaggagcgcc gtcgcgagac
     1141 cgagaaccgg ttgaaggaca cgcgcgagaa cctcacgcgc gtcgaagaca tcctgcgcga
     1201 gctgaacaac aacctcgcgc acctggaaaa gcaggccgag gtcgctacgc aataccgcgg
     1261 cctgcaggag cagggcacgc tcaagctgca tcagctgtgg ttcctgaagc accgcgacgc
     1321 ggcgggcgag gaagagcgcg taaaaaaggc ggtgctcgag gcgaccaatg cgctcgaatc
     1381 gcgcctggcc gagctgcgcc atgtcgaggc cgagctggag caggtgcgcc aggcccatta
     1441 ccgggccagc gacgatctgc atgcggccca gggcacattg gccgaggccg cgctggaagt
     1501 gagcaggctc gaagagcgca tccgctatgt ggtcgagggc cgtgcccggg tcgaggcgag
     1561 gctggccgag ctcaaggcgc agaacgagca atggggcgag cgtcggcaac aggcccagga
     1621 tgagctggag catattgccg agcagatcgc cgctgccgag gagcaggccg agctgctggc
     1681 ggcacaggcc gaagagcagt ccggcgccct gccgaaccac gaagacgcgg tgcgcgccgc
     1741 ccagtccaag agcaacgagc agcgcggcag cgtggcccaa gtgcagcagc agatccagct
     1801 gctggcggcc gaatcgcgca acatcgacga gcagggccgt gcgttggcgc aaaggcgcga
     1861 gcggctggcg agcgagcgtc aaggcctggc ccagccggac ggggcgcgca tggcggagtt
     1921 gaagcagcaa ctcgccgatg ctgaagagtc gcaggcgatg gcagaggccc gcctgcacga
     1981 gctgaccgag caggtgcccg cgctcgacga ggaccggcgc gcccgccagg agcaggtgaa
     2041 tgccgaggcc gccaggcagg ccgacctgaa cgcccgcctc gaagcgctgc gcgcgctgca
     2101 ggagaaggtg cagaccgaag gcaagctcaa gccctggctc gccaagcatg gtctggatgg
     2161 gctggcgggc ctttggacca aggtgcacat cgagaccggc tgggaaacgg cgctggaatc
     2221 tgcgctgcgc gagaaactca acgcgctcga ggtcggccgc gtcgagaccg tgcgcgcctt
     2281 cgagaacgac gcgccgcccg ccaagctggc cttctatacc gcgccggccg gtgtcatcgc
     2341 caacacgcac cagacgctgc cacgcctctc cgatctgcta cgcctgggag acgctggcct
     2401 caaggccttg ctcaacgact ggcttgaggg cgtctacacc gccggctcgc tggacgaagc
     2461 tctggccagc cgcagtaagc tcacgcacgg tgaagtgatc atgacgaagg cgggccatgc
     2521 ggtgtcgcag ttcgccgtgg ccttctatgc gcccgattca gagcaggccg gcatgctggc
     2581 gcgcgcccag gaaatcgaaa accttgagaa gcagacgcgc gcgcagtcct tgctggtcga
     2641 ggaggcacgc agcgcgctgg tgcgcgccga ggccgcttac accgaggcct cgcaggggct
     2701 ggtgagcgcg cggcgtgaag ccgccgaggg ccagacgcgt acgcatcagc tgcaggtcga
     2761 gacgctgcgc ctgacgcagc tggccgagca gaccagccag cgccgcggcc agatcgaagg
     2821 cgagctggcc gagatcgacg ccggcctcga ggagctgcag gagcgccgca tcaccggcga
     2881 agcgcgcttt gaggagctgg acatgcagct cgccaacgaa caggagcgcc atgccgagct
     2941 ggacgaggcg gtgatccagg ccgagcgccg gcaaggcgag gcgcgcgagc agctgcgctc
     3001 gctggagcgc caggcccagg aggcgcaatt ctcggcgcgc tcgctggcgg cgcgccgcgg
     3061 cgaactgcag cgctccatcg agcaggcagt gcagatgatc gcggccaacg agcaatcggc
     3121 cacgcaattg cacgacgagc ttgcgcaatt caatgaccag gccgcccagg ccgggctgca
     3181 ggaggcgctg gcgctgaagc tggagcgcga aggcgccctg gccgcgcagc gcagccaata
     3241 cgacgacctg tcggcccagc tgcgtcgagc ggatgagcag cgcctggaat tcgagcgcag
     3301 cctcgacccc ttgcgtgagc gcatcaccaa gctgcagctg gaagagcagg cggcccagct
     3361 cggcggcgcc cagttcatgg agcagctcac ggcggcccag gtcgatctgg aagccctggc
     3421 ccgcagcatc gaagaaggcg gcgtcaagct ctacgggctg cagggcgaga tcgaccgcat
     3481 caaccgcgat atcaattcgc tcggcgcagt gaacctggcc gcgctggacg agctggcggc
     3541 ggcgcgcgag cgcaagacct tcctcgacgc gcagtcagcc gatcttgtcg aggcgatgaa
     3601 cacgctggaa ggcgcgatcc acaagatcga cctggaaacc cgcgatctgc tgggctctac
     3661 cttcaacacg gtcaacgagc atttcggccg catgttcccc agcctcttcg gcggcggcca
     3721 ggccaagctc gtgatgacgg gcgacgagat cctcgatgcc ggcgtgcagg tgatggccca
     3781 gccgccgggc aagaagaaca gcaccatcca tctgctgtcg ggcggcgaga aggcgttgac
     3841 cgcaatcgcg ctggtgttcg cgattttcca gctcaatccc gcgcctttct gcctgctgga
     3901 cgaggtggac gcgccgctgg atgatgccaa caccgagcgc tattccaagc tcgtcaagag
     3961 catggccaac aacacgcagt ttctcttcat cagccacaac aagatcgcga tggagatggc
     4021 cgaacagctg atcggcgtga ccatgcagga gcaaggcgtg tcgcgcatcg tggcggtgga
     4081 catggaagcc gccgtcagca tggcggaagc ggcctgagcc agcgccgtca acaccaggca
     4141 gcccgggcat gagttccctg caagtctccc tggcagtcct gggtggcctc gtgctggccg
     4201 gcatcgtggc ccatagcgcc tggcaggcgc gccgggcggg cgtaaggacc gcgccgcaat
     4261 ccgctgcgcg ccaggagccg cgcgagcccg tcctcggcga cggcgcaacg ccccctgctg
     4321 cgccgcagat ggctggcgag accgagccag ccgccacgct cggccaggag cgcctcgatc
     4381 tgccgccttt gctgcggcgt tccacggcga ggctggatgc cctgatcgac gcgatcgcat
     4441 cgttgacgct ggaggcgccg gtcagcgccg agactgcgct gttgcatctg ccgccctcgc
     4501 ggcgcgccgg cagcaagccc ttcatgatcg agggtctgaa cactgagagc ggcgactggg
     4561 agctgcccgc gctgggccag cgctatggcg agttccaggc cggcgtgcag ctcgccaacc
     4621 gcaccggcgc gctgaacgag atcgagtact ccgagttcgt gcagaaggtg caggccttcg
     4681 ccgaggcggt gggcgcgatg gccgacttcc ccgacatgct cgacgtggtg gcgcgcgcgc
     4741 gcgagctcga cgccttcgcc ggcgaccacg acgcgcagct cgcggtgcac ctgcgctcca
     4801 aggcggtggc gtggagcgtg ggctacatcc agcagcacgc cgggtggcac ggcttcgtgg
     4861 cgggcgtggt gccgggccgc ctcgtgctgc cgtccgcgga ggagggcgcg ccgccggtgc
     4921 tgaccctcac cttcgactcg caggccgcgc tcgccgacga cccgggccgc gccgcggtgc
     4981 gcgacgtga
//
LOCUS       DASUCY010000363         4171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_875324, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000363 DASUCY010000000
VERSION     DASUCY010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4171
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875324"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VFV25_07200"
     CDS             complement(<1..621)
                     /locus_tag="VFV25_07200"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY1393146.1"
                     /translation="MSYLPGKFVWFEHVSAEPTKARAFYEPLFGWHVERMPMGQASYD
                     MILCGQDGIGGIPPAQAGARAHWISYLSVPDVDKAHAKALAAGAKSLQAPTDFAPVGR
                     GAKLSDPTGAAFWVWKSADGDRADAEQAIPGSWAWNELMTQDAKTALAFYEKAFGFTH
                     DTMDMGEQGKYYILKSADGKGRAGLAQASDPKAPAAWMPYVHVADCD"
     gene            complement(809..1324)
                     /locus_tag="VFV25_07205"
     CDS             complement(809..1324)
                     /locus_tag="VFV25_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009980970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HEY1393147.1"
                     /translation="MQARLVFVSPAIRLWMAGLFALLLGVTMLSGCAALLYHDPIRVN
                     VVGIGPVAGEGLELRFAVKLRLQNPNDIDLSFDGLSLELQLAGMPVATGVSNQKGMVP
                     RFGDTVLSVPVSISVLAAARQAITMAGSQQPLPYVLTGKLAGGVFGTMRFRKEGTVNW
                     GGDKSGRGESS"
     gene            1644..2669
                     /locus_tag="VFV25_07210"
     CDS             1644..2669
                     /locus_tag="VFV25_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEY1393148.1"
                     /translation="MTSQPLPELLELQRLIAEGGSALQARVVCEAESGGERYPVHVIT
                     LGNASPQVPAIGYFGGVHGLERIGAEVVMAHLRSLVKRLQWDSLLHQQLQGLRLVFMP
                     LVNPGGLARGTRANPRGVDLMRNAPVECAERAPFLLGGQRLSAGLPWYRGAKDAPMEA
                     ESAALCRVVEEELLSRRFSIAIDCHSGFGTRDRIWFPYAHTTRPIEHLAELHALNLIF
                     DQTHPHHRYVFEPQSRQYLAHGDLWDYLYLRACEQPGRILLPLTLEMGSWLWVKKNPR
                     QLFSRQGIFNPLIEHRQQRVLRRHMAWLDFAARAAGSCERWLPSAAARERHHALALQR
                     WYARVSA"
     gene            2666..3448
                     /locus_tag="VFV25_07215"
     CDS             2666..3448
                     /locus_tag="VFV25_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY1393149.1"
                     /translation="MSIWILLRGLTREARHWGDFPSLLGAELPGAQVLALDLPGNGSL
                     HGMKSPTRVEAMAEHCRRELRERGMAPPYHLLAMSLGAMVAVAWGHAHPEELAACVLV
                     NTSLRPYSPFHERLRPGSYADLLRIALWPGGARQWEESVLKLTSRSHAPGSAQREALL
                     QRWVSWHREQPVSRANAARQLLAAGRYRAPQSRLAPPTLLLTSERDALVSTRCSIRLA
                     EAWQCAIASHAQAGHDLPLEDGTWVARQIALWLQTGVAYSST"
     gene            3474..3848
                     /locus_tag="VFV25_07220"
     CDS             3474..3848
                     /locus_tag="VFV25_07220"
                     /inference="COORDINATES: protein motif:HMM:NF018050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY1393150.1"
                     /translation="MAIYTTRWFDRWARNQGLVASSLCTAVHEMTNGLFDADLGGGLF
                     KKRIARPGQGKSGGYRTLVATNKGNRWVFMYGFPKNERSNIDKDEEEALKKLAAHLLS
                     LTAQAVTKAKQAGELIEVDCDA"
     gene            3841..4155
                     /locus_tag="VFV25_07225"
     CDS             3841..4155
                     /locus_tag="VFV25_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HEY1393151.1"
                     /translation="MRKTKSPILEAVHETAKGLHKAGVMNQVTLREFDRLCLPPVEPL
                     QPEEIKKIREASHVSQAVFARLLNTSLSTVQKWEIGQKRPTGTALKLLHLVQKRGLEV
                     VA"
BASE COUNT          683 a         1364 c         1452 g          672 t
ORIGIN      
        1 gtcgcaatcg gccacgtgca cataaggcat ccaggccgcg ggggccttgg ggtccgaggc
       61 ctgggccagg ccggcgcggc ccttgccgtc ggcgctcttg aggatgtagt acttgccctg
      121 ctcgcccatg tccatcgtgt catgggtgaa gccgaaggcc ttctcgtaga aggcgagcgc
      181 ggtcttggcg tcctgcgtca tcagctcgtt ccaggcccag ctgccgggaa tggcctgctc
      241 ggcatcggca cggtcgccgt cggcgctctt ccatacccag aaggcggcgc cggtcggatc
      301 gctgagcttg gcgccgcggc cgacgggggc gaagtccgtg ggggcctgca acgacttcgc
      361 gccggccgca agcgccttcg catgggcctt gtccacatca gggaccgata ggtaggagat
      421 ccaatgcgcg cgggcgccgg cctgggcggg cgggatgccg ccgatgccgt cctggccgca
      481 caggatcatg tcgtagctcg cctggcccat gggcatgcgc tccacatgcc aaccgaacag
      541 cggctcgtaa aaggcgcgcg ccttggtggg ctcggccgac acatgctcga accagacgaa
      601 ttttccgggc agatagctca tggctggatc ctttcggtag gttcaccacg accggtgaat
      661 cgctgatctt gcgaaccggt cgtgacaggt ctgtggctgc gcgaaaaagg gccaggcgca
      721 cctggcctgc ttcttccgca caaggctaac catagaccat tggaggcccg agcgacaggc
      781 cgttcacgtg aagagtcgtt agctcgactc agctcgattc gccgcggccc gacttgtcgc
      841 cgccccagtt caccgtgccc tccttgcgga agcgcatggt gccgaagacg ccgccggcca
      901 gcttgccggt cagcacatag ggcagcggct gctgcgagcc ggccatcgtg atcgcctggc
      961 gcgcggcggc gagcaccgag atgctgacag gcaccgacaa gacggtgtcg ccgaagcgcg
     1021 gcaccatgcc cttctggttg ctcacgccgg tggcgaccgg catgccggcc agttgcagct
     1081 ccagcgacag tccgtcgaag gagaggtcga tgtcgttggg gttctgcagc ctgagcttca
     1141 cggcgaagcg cagctccagc ccttcgccgg ccaccggccc gatgccaacg acattgacgc
     1201 gtatagggtc gtgatagagc agggccgcgc agccgctcag catggtgacg cccagcagca
     1261 gggcgaacag gccggccatc catagccgaa tggcgggaga aacgaacacg aggcgagctt
     1321 gcatgcgagg gccttccttg gcagcgggat gccaaagtat gcccatgccg cgcagcagcg
     1381 gactaagtgc ggcgacggtg cgaccagcgc gcgcggctga gcgcgatcca ctcggcgatg
     1441 ccccacagca gggcgcaggc cagggccgag gcgacgagaa ggacggggcg gctgctgttc
     1501 atggggcgcg ggctgcaagc gggaccgcca cactagtccc gcgatgtgac gtttccgtga
     1561 cagcggtgcc cgtgcaaggc cgggtccgct cgcgcaggct gcgggttatt cttgtgcgct
     1621 tattccctcc gccgccgcgc tgcgtgactt cccaaccctt gcccgagttg ctcgagctgc
     1681 agcgcctcat tgccgaaggc ggctccgcgc tgcaggcccg cgtcgtgtgc gaggcggaaa
     1741 gcggcggtga acgctacccg gtgcacgtga tcacgctggg caatgcctcg ccgcaagtgc
     1801 cggcgatcgg ctacttcggc ggcgtgcacg gcctggagcg catcggcgcc gaggtggtga
     1861 tggcccatct gcgcagcctg gtgaagcggc tgcaatggga cagcctgctg caccagcagc
     1921 tgcagggcct gcgcctggtc ttcatgccgt tggtcaatcc cggcggcctg gcgcgcggca
     1981 cgcgggccaa tccgcgcggc gtggacctga tgcgcaatgc cccggtggaa tgcgccgagc
     2041 gcgcgccctt cctgctcggc gggcagcgct tgagcgccgg cctgccctgg taccgcggcg
     2101 ccaaggacgc gccgatggag gccgagagcg cggcgctgtg ccgcgtggtg gaagaggagc
     2161 tgctgagccg gcgcttcagc atcgccatcg attgccactc gggcttcggc acgcgcgacc
     2221 gcatctggtt tccctatgcg cacaccacgc ggcccatcga gcacctggcc gagctgcacg
     2281 cgctcaatct gatcttcgac cagacgcatc ctcaccaccg ctatgtgttc gagccgcaga
     2341 gccgccaata cctcgcgcac ggcgatctct gggactacct ctacctgcgc gcctgcgagc
     2401 agccgggccg catcctgctg ccgctgacgc tggagatggg ctcctggctg tgggtcaaga
     2461 agaatccgcg ccagctcttc tcgcgccagg gcatcttcaa tccgctgatc gagcaccgcc
     2521 agcagcgcgt gctgcgccgg cacatggcct ggctggactt cgcggcgcgc gccgccggca
     2581 gctgcgagcg ctggctgccc agcgccgcgg cgcgcgagcg tcaccacgcg ctggccttgc
     2641 agcgctggta tgcgagggtg tcggcatgag catatggatc ctgctgcgcg gcctgacgcg
     2701 cgaagcgcgg cattggggcg actttccctc gctgctgggt gccgagctgc cgggcgcgca
     2761 ggtgctggcc ctggacctgc ccggcaacgg cagcctgcat ggcatgaaga gccccacccg
     2821 cgtggaggcg atggcggagc actgccgccg cgagctgcgc gagcgcggca tggcgccgcc
     2881 ctatcacctg ctggcgatgt cgctcggcgc gatggtggcg gtggcttggg gccatgcgca
     2941 tcccgaggag ctcgcggcct gcgtgctcgt caacaccagc ctgcgcccat acagcccctt
     3001 ccacgagcgc ctgcgcccgg gcagctatgc agacctgctg cgcattgcgc tgtggcccgg
     3061 cggcgcgcgg caatgggaag agagcgtgtt gaagctcacc tcgcgcagcc acgcgcccgg
     3121 ctcggcgcag cgcgaggcct tgctgcagcg ctgggtgagc tggcaccgcg agcagccggt
     3181 gtcgcgtgcc aatgcagcgc gccagctgct ggcagcgggc cgctatcggg cgccgcaaag
     3241 ccggcttgcg ccgccgacgc tgctgctgac cagcgaacgc gacgcgctgg tcagcacgcg
     3301 ctgctcgatt cgcctggccg aggcctggca atgcgcgatc gcgagccatg cgcaggccgg
     3361 acatgacctg ccgctggagg atggcacctg ggtcgcgcgg cagatcgcgc tgtggttgca
     3421 gactggtgtt gcatacagta gcacctagtg ctataaatcc ttggaggggg cgtatggcga
     3481 tctacacgac ccgatggttc gaccgttggg cgcgcaatca gggactggtc gcgtcaagcc
     3541 tctgtacagc cgtgcatgaa atgacgaacg ggttgttcga tgcagacctc ggcgggggtc
     3601 tgttcaagaa gcgtatcgct cgtcctggcc agggaaagag cggtggctac cggacgctgg
     3661 tcgctaccaa caagggcaat cgctgggtct tcatgtacgg cttcccgaag aacgaacgca
     3721 gcaacatcga caaggacgaa gaggaagcgt tgaagaagtt ggcggctcac ctgttgtcgt
     3781 tgaccgcgca ggctgtcacg aaagccaagc aggcgggcga attgattgag gtggactgcg
     3841 atgcgtaaga cgaaatcccc cattctcgaa gccgtgcatg agacggcgaa gggcctgcac
     3901 aaggcgggcg tgatgaatca ggtcacgctg cgtgagtttg accggctgtg cctgccgccg
     3961 gtcgagccgt tgcagccgga agagatcaag aaaattcggg aggcctcgca tgtcagccag
     4021 gctgtcttcg cgcgcctgct aaacaccagc ctctcgaccg tccagaagtg ggagatcggc
     4081 cagaagcggc ccacaggcac ggccttgaag ctgctgcacc tggttcaaaa gcgagggctg
     4141 gaggttgttg cctagtcggc ggcatgctgc c
//
LOCUS       DASUCY010000364         2057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1517849, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000364 DASUCY010000000
VERSION     DASUCY010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1517849"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="VFV25_07230"
     CDS             <1..755
                     /locus_tag="VFV25_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY1393152.1"
                     /translation="NEIGVIQDIPAIGALCRSKGVLFHVDAAQATGKVAIDLSTLPVD
                     LMSLSAHKTYGPKGIGALYVRRKPRVRIEAQMHGGGHERGMRSGTLPTHQIVGMGEAY
                     RIARQSMGAEGERIRALRNRLLEGLQGIPEVRVNGDLERRVAHNLNVSFTFVEGESLL
                     MGMKGIAVSSGSACTSASLEPSYVLRALGLPDEVAHSSIRFSVGRFTSEAEIDAAVER
                     VKATVGRLRELSPLWDMHCAGVDLSQVQWAEH"
     gene            805..1233
                     /gene="iscU"
                     /locus_tag="VFV25_07235"
     CDS             805..1233
                     /gene="iscU"
                     /locus_tag="VFV25_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HEY1393153.1"
                     /translation="MAYSDKVIDHYENPRNVGGFTTEDDDVGTGMVGAPACGDVMKLQ
                     IKVNPATGLIEDAKFKTYGCGSAIASSSLVTEWVRGKTLDQALAIKNTAIAEELALPP
                     VKIHCSILAEDAIKAAVADYRQRHDGHLEQPACDPTQVTA"
     gene            1330..1695
                     /gene="erpA"
                     /locus_tag="VFV25_07240"
     CDS             1330..1695
                     /gene="erpA"
                     /locus_tag="VFV25_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEY1393154.1"
                     /translation="MPPAAPFVLPEGAAPLVISEAVVRKVGDMLAEEGDSNLKLRIFI
                     TGGGCSGFQYGFAFDDEVKPDDTCVEAGAIKVLVDAASLQYVAGAEIDYEENLEGSRF
                     VIRNPNAASTCGCGSSFSV"
     gene            1718..2041
                     /locus_tag="VFV25_07245"
     CDS             1718..2041
                     /locus_tag="VFV25_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEY1393155.1"
                     /translation="MSLSVTPKAARQIRKALDQRGNGYGLRVAVKTSGCSGYSYALEF
                     ADAPASDDLSFESEGVRLLVDVKSLPMIDGTQLDWVREGLNEGFKFNNPNASATCGCG
                     ESFAV"
BASE COUNT          374 a          698 c          677 g          308 t
ORIGIN      
        1 acaacgagat cggcgtgatc caggacattc cggccatcgg cgcgctgtgc cgcagcaagg
       61 gcgtgctctt tcacgtcgat gccgcgcagg ccaccggcaa ggtggccatc gatctctcca
      121 cgctgccggt ggacctgatg agcctgtctg cccacaagac ctacggcccc aagggcatcg
      181 gtgcgctcta tgtgcgccgc aagccgcgcg tgcgcatcga ggcgcagatg cacggcggcg
      241 gacacgagcg cggcatgcgc tcgggcacgc tacccacgca ccagatcgtc ggcatgggcg
      301 aggcctatcg aatcgcgcgc caatccatgg gcgccgaagg cgagcgcatc cgcgcgctgc
      361 gcaaccgcct gctcgaaggg ctgcaaggca ttcccgaggt gcgcgtcaac ggcgacctgg
      421 agcggcgcgt ggcgcacaac ctcaacgtga gtttcacatt cgtcgagggc gagtcgctgc
      481 tgatgggcat gaagggtatc gcggtgtcat ccgggtcggc ctgcacatcc gccagcctgg
      541 agcccagcta cgtgctgcgc gcgctgggcc tgcccgacga ggtggcgcac agctccatcc
      601 gcttctcggt aggccgcttc accagcgagg ccgaaatcga cgccgcggtc gagcgcgtga
      661 aggccacggt ggggcgcctg cgcgagctca gcccgctgtg ggacatgcat tgcgccggcg
      721 tcgatctcag ccaggtgcaa tgggcagaac attaggaatg gtgccccccc gggggcccca
      781 cggcaatctg aacggagcga agaaatggcc tacagcgaca aagtcatcga ccactacgag
      841 aacccgcgca atgtcggcgg cttcaccacc gaggacgacg acgtcggcac cggcatggtt
      901 ggtgcgccgg cctgcggcga cgtgatgaag ctgcagatca aggtcaaccc cgccaccggg
      961 ctgatcgaag atgcgaagtt caagacctac ggctgcggct cggccatcgc ctccagctcg
     1021 ctggtgaccg aatgggtcag gggcaagacg ctggaccagg cgctggcgat caagaacacc
     1081 gccatcgcgg aggagctggc gctaccgcct gtgaagatcc actgttccat cctcgccgag
     1141 gacgcgatca aggccgcggt ggccgactac cgccagcgcc acgacggcca cctggagcaa
     1201 cccgcctgcg atcccacgca ggtcacggcc tgaacgccgc gccttcactc acccatcccg
     1261 caaggagctc tgcccatgtc cgcctgccag tcccaaccca acgccggttc ttcctgttcg
     1321 accggcggca tgccgcccgc agcccccttc gtactgcctg agggcgcggc gccgctggtc
     1381 atcagcgaag ccgtggtccg caaggtcggc gatatgctgg ccgaggaggg cgactccaac
     1441 ctcaagctgc gcatcttcat caccggcggg ggctgctcgg gcttccagta cggcttcgcc
     1501 ttcgacgacg aggtcaagcc cgacgacacc tgcgtcgagg ccggagcgat caaggtgctg
     1561 gttgatgcgg ccagcctgca gtacgtggcc ggcgcggaga tcgactacga ggaaaacctc
     1621 gaaggctcgc gcttcgtgat ccgcaacccc aacgcggcca gcacctgcgg gtgcggcagc
     1681 tcgttctcgg tctgagcacc gcaagcagga ggccaccatg tctttgagcg tgacacccaa
     1741 ggccgcccgg cagatccgca aggcgctgga ccagcgcggc aacggctatg gcctgcgcgt
     1801 ggcggtgaag accagcggct gctccggcta ttcctacgcg ctggaattcg ccgatgcgcc
     1861 ggctagcgac gacctgagct tcgagagcga aggcgtgcga ctgctggtcg atgtcaagag
     1921 cctgccgatg atcgatggca cccagctcga ctgggtgcgc gaaggcctga acgagggctt
     1981 caagttcaac aaccccaacg cgtcggcgac ttgcggctgc ggtgagagct tcgcggtctg
     2041 acatggccgc ttaccgt
//
LOCUS       DASUCY010000365         3363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1260858, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000365 DASUCY010000000
VERSION     DASUCY010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1260858"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="VFV25_07250"
     CDS             <1..419
                     /locus_tag="VFV25_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HEY1393156.1"
                     /translation="CGVDTLLAAQAHGGMAALRAKSLALTDLFVELVESRCAGQGLSL
                     VTPREHALRGSQACFSRAEGAYAIVQALIARGVVGDFRAGDPANPAGAQDILRFGFTP
                     LYTSYVEVWDAVEQLVQVLASGEWREASFNQRAAVT"
     gene            430..1278
                     /gene="kynA"
                     /locus_tag="VFV25_07255"
     CDS             430..1278
                     /gene="kynA"
                     /locus_tag="VFV25_07255"
                     /EC_number="1.13.11.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase"
                     /protein_id="HEY1393157.1"
                     /translation="MTTKARPADIVRDEKAQLDFSKDMSYGDYLQLDRLLSAQKPLSP
                     EHDEMLFIVQHQTSELWMKLMLHELRAAIAAIAADELAPAFKMLARVSRIMEQLVHAW
                     DVLATMTPPEYSAIRPYLSNSSGFQSWQYRCIEFALGNKNAEMLKPHAHSPQRLAEVQ
                     AAFEAPSLYDEALRLLARRGLAVPASHTERDWTQPYVESTAVEQAWLFVYRDPAHHWD
                     LYQLGEELTDLEDTFRLWRFRHVTTVERVIGFKRGTGGTSGVSYLRKMLDVVLFPEIW
                     KLRTDL"
     gene            1365..1826
                     /locus_tag="VFV25_07260"
     CDS             1365..1826
                     /locus_tag="VFV25_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393158.1"
                     /translation="MKITLPRRASGLPGLPLGALLLLSCLAAWPARAAELSISITNAP
                     IEEAQAKKIVSFALPADFDRIFPAKQYGVRVLVDEHQGRDGGVVFYMLLGLAHRLPDG
                     RFLQEHTYLSSMEFVKPTAAAGDRRAILEGALAQSARLFAQSMMENAARLK"
     gene            complement(1839..2288)
                     /locus_tag="VFV25_07265"
     CDS             complement(1839..2288)
                     /locus_tag="VFV25_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEY1393159.1"
                     /translation="MSKISSKLRNFGAVFASVLAAAVALPGAAYAAHLPAEHRLNGGV
                     GYVSGGIGDDEALRFKAAAKDFPLVIELFRHDGKRDDYTADARVTISDANGHVVLNEQ
                     ADGPFMLVRLPAGRYTVSSSLDGRTLPAQHVNVAATGSTRSVFVFPG"
     gene            complement(2345..>3363)
                     /locus_tag="VFV25_07270"
     CDS             complement(2345..>3363)
                     /locus_tag="VFV25_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEY1393160.1"
                     /translation="REFRAKVLGSDSATDIAVLRIDAKNLPSVRLDDPDQLQVGDPVL
                     AIGTPFGFEHSATQGIVSAKGRSLPGDGYVPFIQTDAAVNPGNSGGPLFAADGGVVGI
                     NAQIYSRTGGFEGLSFAIPINVALKVKDQILATGHVRHARLGVTIQDLSQPLADSFGL
                     PRPDGALVGSVSPDSAAAAAGLRSGDVVTEINGQPVLSSGDLSSRIGLMAPGEKVRLK
                     VWRGKSSREVDVKLGSADDGGQQSASNDGGAQGEQLGLALRPLTREEARQEHVNSGLV
                     VEGVDGPAARAGIQPGDVVLAVNGKPVSSVQQVRDVLAQKPKSVALLIERDGDRIFVP
                     VRLG"
BASE COUNT          532 a         1165 c         1126 g          540 t
ORIGIN      
        1 aatgcggcgt ggatacgctg ctcgccgctc aggcccatgg tggcatggcc gcactgcgtg
       61 ccaagtcgct ggcgctgacc gatctcttcg tcgagctggt cgagtcgcgc tgcgccggcc
      121 agggcctgag cctcgtgacg ccgcgcgagc acgcgctgcg cggcagccag gcctgcttct
      181 cgcgcgccga aggcgcctac gccatcgtgc aggcgctgat cgcacgcggc gtggtcggcg
      241 acttccgcgc tggcgaccct gccaatcccg ccggcgcgca ggacatcctg cgcttcggct
      301 tcacgccgct gtacaccagc tacgtggaag tctgggatgc agtggagcag ttggtacagg
      361 tgctggccag cggcgaatgg cgcgaagcga gcttcaacca gcgcgcggca gtgacttgag
      421 gttcaggcag tgacgacaaa agcaagaccc gcggacatcg tccgcgacga gaaggcccag
      481 ctcgacttct ccaaggacat gagctatggc gactatctgc agctcgaccg gctgctgtcg
      541 gcgcagaagc cgctctcgcc cgagcacgac gagatgctct tcatcgtgca gcaccagacc
      601 tcggagctgt ggatgaagct gatgctgcac gagctgcgcg cggccatcgc cgcgatcgcg
      661 gccgacgagc tggcgccggc cttcaagatg ctggcgcgcg tttctcgcat catggagcag
      721 ctcgtgcatg cctgggacgt gctggccacg atgacgccgc cggagtactc ggccatcaga
      781 ccctatctca gcaacagcag cggcttccag agctggcagt accgctgcat cgagttcgcg
      841 ctcggcaaca agaacgcgga gatgctcaag ccgcacgcgc acagcccgca acggctggcc
      901 gaggtgcagg cggccttcga ggcgccctcg ctctatgacg aagcgctgcg cctgctggcg
      961 cgccgcggcc tggccgtgcc ggccagccat accgagcgcg actggaccca gccctatgtc
     1021 gagagcaccg ctgtcgagca ggcctggctg ttcgtgtatc gcgatcccgc gcatcactgg
     1081 gatctctatc agctcggcga ggaactcacc gatctggaag acaccttccg cctctggcgc
     1141 ttccgccatg tcacgacggt ggagcgcgtg atcggcttca agcgtggcac cggcggaacg
     1201 tcgggcgtga gctatctgcg caagatgctg gacgtggtgc tcttccccga gatctggaag
     1261 ctgcgcaccg atctgtgagt gcgcaggcgc aggcgccggc atcgggcatt gcgccccgcc
     1321 ctgcgtctgg ctatcgttga gaaccatacc ttgcggaggc caatatgaag atcaccctgc
     1381 cccgccgcgc cagcggtttg cccggtttgc cgctcggcgc cctgctgctg ctgtcctgcc
     1441 ttgccgcctg gcccgcccgg gccgccgagc tgagcatcag catcaccaat gcccccatcg
     1501 aagaggcgca ggcgaagaag atcgtgagct tcgcgctgcc ggccgacttc gaccgcatct
     1561 tccctgccaa gcagtacggc gtgcgcgtgc tggtcgacga gcaccagggg cgcgatggcg
     1621 gcgtcgtgtt ctacatgctg ctgggcctgg cccatcgcct gcccgatggg cgcttcctgc
     1681 aggaacacac ctatctgagc agcatggaat tcgtcaagcc caccgccgcc gccggcgacc
     1741 ggcgcgccat cctcgagggc gcgctggccc agtcggcgcg gctgttcgcg cagtcgatga
     1801 tggagaacgc ggcccgcttg aaatagcgcc gtggatcatc agccggggaa cacgaagacg
     1861 ctgcgcgtcg aacccgtggc ggccacgttc acgtgctggg ccggcagggt tctgccgtca
     1921 agcgagctgc tgaccgtgta gcggcccgcg ggcaggcgca ccagcatgaa gggcccgtcg
     1981 gcctgctcgt tcagcaccac gtggccgttc gcatcgctga tcgtcacgcg ggcgtcggcc
     2041 gtgtagtcgt cgcgcttgcc gtcgtggcgg aacagttcga tgacgagcgg aaagtccttg
     2101 gccgccgcct tgaatcgcag cgcctcgtcg tcgccgatgc cgcccgagac ataacccacg
     2161 ccgccgttca agcgatgctc ggcgggcaga tgggcggcgt aagccgcgcc gggcagcgcg
     2221 accgccgccg cgagcaccga cgcgaacacg gcgccgaagt tgcgcaattt gctgctgatc
     2281 ttgctcatga tgtttccttc cttttctggc acatgacggc ccgccgggtc gcggatgaaa
     2341 ccgctcagcc cagcctgacg gggacgaaga tgcggtcgcc gtcgcgctcg atcagcaagg
     2401 cgacgctctt gggcttttgc gccagcacgt cgcggacctg ctgcaccgag ctgaccggct
     2461 tgccgttgac ggccagcacc acgtcgccgg gctggatgcc ggcgcgcgcg gccgggccat
     2521 ccacgccctc gacgaccagg ccactgttca catgctcctg gcgcgcttcc tcgcgcgtca
     2581 gcgggcgcag ggcgaggccc agttgctcgc cttgcgcgcc gccatcattg gaagcggact
     2641 gctggccgcc atcgtcggca ctgccaagct tcacgtccac ctcgcggctg gacttgccgc
     2701 gccagacctt gagcctgacc ttctcgccgg gcgccatcag gccgatgcgg ctggacaggt
     2761 caccggagct cagcacgggc tggccgttga tctcggtgac cacatcgccc gacctgaggc
     2821 cggccgcggc cgccgcgctg tccgggctca cgctgccgac cagggccccg tcgggccgcg
     2881 gcaggccgaa ggaatcggcc agcggctggc tcaggtcctg gatcgtcacg cccaggcggg
     2941 cgtgccgcac atggccggtg gcgaggatct ggtccttgac tttgagcgca acattgatcg
     3001 ggatcgcgaa ggacaggccc tcgaagccgc cagtgcgcga gtagatctgg gcgttgatgc
     3061 cgaccacgcc gccatcggcc gcaaacagcg ggccgcccga gttgccggga ttgaccgccg
     3121 catcggtctg aataaagggc acatagccgt cgccgggcag cgaacggccc ttggcgctca
     3181 cgatgccctg cgtggccgaa tgctcgaagc cgaagggcgt gccgatcgcc agcaccgggt
     3241 cgccgacctg cagctgatcg ggatcgtcca acctgacgct gggcaggttc ttggcgtcga
     3301 tgcgcagcac cgcgatatcg gtggccgagt cggaacccag caccttggcg cggaactcgc
     3361 gcc
//
LOCUS       DASUCY010000366         4644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_763111, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000366 DASUCY010000000
VERSION     DASUCY010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763111"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..545)
                     /locus_tag="VFV25_07275"
     CDS             complement(105..545)
                     /locus_tag="VFV25_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY1393161.1"
                     /translation="MPKTQLDDTDRALLALLRENAREPVAELARRLHLSRTTVQSRIE
                     RLERARVITGYTVKVADEIEAALVRAHVMITVAPKQAGAIEAACAKIAEVRSLHSVSG
                     SFDLLAVVAAPSIGELDALIDRIGALDGVERTTSAIVLSTRIER"
     gene            665..1792
                     /locus_tag="VFV25_07280"
     CDS             665..1792
                     /locus_tag="VFV25_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEY1393162.1"
                     /translation="MKSILLLGGGKIGSVVADLLAHASTAGQPDYRVTVADRSEALLA
                     GLPRHAQIDTLVLDVTDGAALRAALKGRFAVLSALPYMLTLKVAQAARELGVHYLDLT
                     EDVAATRGIRELAQGANTAFIPQCGLAPGFISIVAHDLARRYESLDSVRMRVGALPAY
                     PSNALNYNLTWSTEGVINEYCEPCEAIVDGKPTEVPALEEREEFSLDGVLYEAFNTSG
                     GLGTLCETLAGKVRTLNYRTIRYPGHAAIMKALLNDLRLRDRRELLKDILENAVPATM
                     QDVVIVFVTVSGIRADESGQKRFLQDTYARKIYSHVLHGTMRSAIQITTASSLCAMLD
                     LLAQGKLPQRGFVMQEDLALADFLGNRFGQVYAARELAQAA"
     gene            complement(1825..2358)
                     /locus_tag="VFV25_07285"
     CDS             complement(1825..2358)
                     /locus_tag="VFV25_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HEY1393163.1"
                     /translation="MQLTSQQLLPVSQQQAWEALNDTTMLQAAIPGCESLEPSGDHEY
                     AVALMAAVGPVKAKFKGKLRLSDLQPPTSYTIQFEGQGGPAGFGKGSASVRLEPTGPN
                     QTTLHYSADAQVGGKIAQIGSRLIDMAAQKMAGEFFESFTRALSERYGVAAPPPAAPA
                     GVLAKFWAWLRRLFGGS"
     gene            complement(2349..3575)
                     /locus_tag="VFV25_07290"
     CDS             complement(2349..3575)
                     /locus_tag="VFV25_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY1393164.1"
                     /translation="MRPDAAIEPPGHLAENVMHFARVLRSAGMPIGTDRIQLALRALQ
                     VAGLRSREDFHATLFSCFVSKAEQRALFDQAFHIFWRDPDLLGRVMAMLLPRAQGKAG
                     APSPPENRRLAQALFPGQGPPSSQQPQERIEFEAAYTWSDREVLRHTDFESMSAEEWL
                     QAKRLLAELRLAFEPLHTRRYQRSSRGGRADWRATLRDMARCGDQPRLSWQRPRLRPP
                     QLVVLADISGSMSRYSRMLLHFAHAATRGEAHTHSFVFGTRLTAITRMLAQRDPDIAV
                     AQVARAVQDWSGGTRISACLHEFNRHWSRRVLGGGNATVLLISDGLEHGDSAALAFEM
                     ERLHKSCRRLVWLNPLLRYDGFEPKAAGIRAMLPHVDHFVPAHNLASLDELARLLRAP
                     APHIQDPATSIEPTCN"
     gene            complement(3586..4503)
                     /locus_tag="VFV25_07295"
     CDS             complement(3586..4503)
                     /locus_tag="VFV25_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY1393165.1"
                     /translation="MTSASALPADIDATLAGLLAQDYVADRQLATALFLALRLQRPLF
                     LEGEPGTGKTEIAKTVAAMLGRPLIRLQCFEGLDLSGAAYEWNYARQMIEIRLAERDH
                     GATREQLTQELFSPRFLIPRALLQAIDPTQAMPPVLLIDELDRADEPFEAFLLEVLSD
                     FQITIPELGTIKAKEPPIVVLTSNRTREIHDAVKRRCLYHWVGFPDAARELAILHRRV
                     PGASEALARQVVAFVQGLRGMELFKLPGIAETIEWTRALMQLDALVLDPETVHNTLGV
                     LLKYQDDIARLQGGAAARLLEQVRAAVPA"
     gene            complement(4516..>4644)
                     /locus_tag="VFV25_07300"
     CDS             complement(4516..>4644)
                     /locus_tag="VFV25_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase"
                     /protein_id="HEY1393166.1"
                     /translation="APESCVGVTVDSGDLNSDLHGSAEYRAALIPVLAQRAVAAAG"
BASE COUNT          733 a         1598 c         1591 g          722 t
ORIGIN      
        1 cgacatggga tcaaggcctc ctcgacgcta cgagattaat cactctaccc aaaacagcaa
       61 caagcaaaca tgacgcgagc gccaagcccc tgcggaaatc aagttcagcg ctcgatccgc
      121 gtggagagca cgatggcgga cgtcgtgcgc tcgacgccgt ccagcgcgcc gatgcggtcg
      181 atcagcgcat ccagctcgcc gatcgatggc gccgccacca cggccagcag gtcgaaggac
      241 ccgctcaccg agtgcaggct gcgcacctcg gcgatcttgg cgcaggccgc ctcgatcgcg
      301 ccggcctgct tgggtgccac ggtgatcatc acgtgggcgc gcaccagcgc cgcctcgatc
      361 tcgtcggcca ccttgacggt gtagcccgtg atgacgcgcg cgcgctccag ccgctcgatg
      421 cggctttgca ccgtggtgcg cgacaggtgg aggcgccgcg ccagctcggc gacgggctcg
      481 cgagcgttct cgcgcagcag ggccagcagg gcgcgatcgg tgtcgtcgag ttgtgttttc
      541 ggcatattga actatcagcg actacgtttc gccgaattgt tgccgacatc tcgtccatag
      601 tacagctgcg ctttgtatgg tctgtgccga caattccgcc atggtctttg aaggaaacac
      661 ccacatgaaa tccatcctcc tgctcggcgg cggcaagatc ggcagcgtcg tggccgattt
      721 gctggctcac gcaagcaccg ctggccagcc cgactaccgc gtcacggtgg ccgatcgttc
      781 cgaagccctg ttggccggcc tgccgcgcca tgcccagatc gacacgctgg tgctggacgt
      841 gaccgacggc gcagccctgc gcgccgctct gaagggccgc tttgccgtgc tcagcgccct
      901 gccctatatg ctgacgctca aggtcgcgca ggccgcgcgc gagctgggcg tgcactacct
      961 cgatctcacc gaagacgtgg ccgccacgcg cggcatccgc gagctggccc agggcgcgaa
     1021 caccgccttc atcccgcagt gcggcctggc gcccggtttc atctccatcg tcgcgcacga
     1081 cctggcgcgc cgctacgaga gcctggactc ggtgcgcatg cgcgtcggcg cgctgccggc
     1141 ctatccgagc aatgcgctga actacaacct cacctggagc accgagggcg tcatcaacga
     1201 gtattgcgag ccctgcgaag ccatcgtcga cggcaagccc accgaggtgc cggcgctgga
     1261 ggagcgcgag gagttctcgc tcgacggcgt gctctacgaa gcgttcaaca cctcgggcgg
     1321 cctgggcacg ctgtgcgaga cgctggcggg caaggtgcgc acgctcaact accgcaccat
     1381 ccgctacccc ggccatgcgg ccatcatgaa ggcgctgctc aacgacctgc gcctgcgcga
     1441 ccgccgcgag ctgctgaagg acatcctcga gaacgccgtg ccggccacga tgcaggacgt
     1501 ggtgatcgtg ttcgtcacgg tcagcggcat ccgtgccgac gagagcggcc agaagcgctt
     1561 cctgcaggac acctatgccc gcaagatcta cagccacgtg ctgcacggca cgatgcgcag
     1621 cgcgatccag atcaccacgg cctccagcct gtgcgccatg ctggacctgc tggcccaagg
     1681 caagctgccg caacgcggct tcgtgatgca ggaagacctg gctctggcgg atttcctcgg
     1741 caaccgcttc ggccaggtct acgccgctcg cgagctggct caggcagcct gaggctcaag
     1801 cggccggtgt gtacgccggc cgcatcagct gccgccgaac aggcggcgca gccaggccca
     1861 gaacttggcc agcacgccgg ccggcgcggc cggcggcggc gccgccacgc cgtagcgctc
     1921 gctcaaggcg cgcgtgaagc tctcgaagaa ttctccggcc atcttctgcg cggccatgtc
     1981 gatcaggcgt gagccgatct gcgcgatctt gccgcccact tgggcgtcgg cgctgtagtg
     2041 cagcgtggtc tggttggggc cggtgggctc cagcctcacg ctcgccgagc ccttgccgaa
     2101 gccggccggg ccgccctggc cctcgaactg gatcgtgtag gaagtcgggg gctgcaggtc
     2161 cgagaggcgc agcttgccct tgaacttggc cttcaccggg ccgacggccg ccatcagcgc
     2221 cacggcgtat tcgtggtcgc cgctgggctc cagcgactcg cagcctggga tcgcggcctg
     2281 cagcatggtc gtgtcgttca gggcctccca ggcctgttgc tgggaaacgg ggagaagttg
     2341 ctggctggtc agttgcatgt cggctcgatc gaggttgcag gatcttgtat gtgcggagcg
     2401 ggcgctctca gcaggcgggc cagctcatcg aggctggcga ggttgtgcgc cggcacgaaa
     2461 tggtcgacat gcgggagcat cgcacggatg ccggccgcct tgggctcgaa gccgtcatag
     2521 cgcagcagcg ggttgagcca aaccaggcgc cggcagctct tgtgcaggcg ctccatctcg
     2581 aaggccagcg cggcgctatc gccatgctcc agcccatcgg aaatcagcag cacggtggca
     2641 ttgccgccgc ccagcacgcg gcgcgaccag tggcgattga attcgtgcag gcaggcgctg
     2701 atgcgcgtgc cgcccgacca gtcctgcacg gcgcgcgcta cctgggccac ggcgatgtcc
     2761 gggtcgcgct gggccagcat gcgcgtgatg gcggtgagcc gggtgccgaa cacgaagctg
     2821 tgcgtatgcg cttcgccgcg cgtcgcggca tgggcgaagt gcaacagcat gcgcgaatag
     2881 cggctcatcg agcccgaaat gtcggccagc accaccagct gcggcggcct caggcgcggg
     2941 cgctgccagc tcaagcgcgg ttggtcgccg caccgcgcca tgtcgcgcag cgtcgcgcgc
     3001 cagtcggcgc ggccaccgcg gctggagcgc tggtagcgcc gcgtgtgcag cggctcgaag
     3061 gccaggcgca gctcggccag caggcgcttg gcctgcagcc attcctcggc cgacatgctc
     3121 tcgaagtcgg tgtggcgcag gacctcgcgg tcggaccagg tgtaggccgc ctcgaactcg
     3181 atgcgctcct gcggctgctg cgagctgggc ggcccctgcc ccggaaacag cgcctgcgcc
     3241 aggcgccggt tctccggtgg cgagggcgcc cccgccttgc cctgcgcacg cggcagcagc
     3301 atcgccatca cgcggcccag caggtcggga tcgcgccaga agatgtggaa ggcctggtcg
     3361 aacagcgcgc gctgctcggc cttcgagacg aagcaggaga acagcgtcgc atggaagtcc
     3421 tcgcggctgc gcaggccggc cacctgcagg gcccgcagcg cgagctggat gcgatccgtg
     3481 ccgataggca tgccggcgct gcgcagcacg cgggcaaagt gcatcacgtt ctcggcgaga
     3541 tggcccgggg gttcgatggc ggcgtctggc ctcatcgcgc gcccatcagg cggggacggc
     3601 agcgcggacc tgctccaaga gccgcgccgc cgcgccgcct tgcagcctgg cgatatcgtc
     3661 ctggtacttg agcagcacgc ccagggtgtt gtgtaccgtt tcagggtcca gcaccagcgc
     3721 gtcgagctgc atcagcgcgc gcgtccattc gatggtctcg gcgatgccgg gcagcttgaa
     3781 cagctccatc ccccgcaggc cctgtacgaa ggcgaccacc tggcgcgcca gggcctcgct
     3841 cgcgcccggc acgcggcgat gcaggatggc cagctcgcgc gccgcatcgg ggaagccgac
     3901 ccagtgatag aggcagcggc gcttgaccgc atcatggatc tcgcgcgtgc ggttggatgt
     3961 cagcacgacg atgggcggct ccttggcctt gatggtgccg agctcgggga tcgtgatctg
     4021 gaagtccgac agcacctcca gcaaaaaggc ctcgaagggc tcgtcggcac ggtccagctc
     4081 gtcgatcagc agcacaggcg gcatggcctg cgtcggatcg atggcctgca acagggctcg
     4141 cggtatcagg aagcgcggcg agaacagctc ctgggtcagt tgttcacgcg tggcgccgtg
     4201 atcgcgctcg gccaggcgga tctcgatcat ctggcgcgcg tagttccact cgtaggcggc
     4261 gccgctcaag tccagcccct cgaagcattg caggcggatc agcgggcggc ccagcatcgc
     4321 cgccaccgtc ttcgcgatct cggtcttgcc ggtgccgggc tcgccttcga ggaagagtgg
     4381 gcgctgcagg cgcagcgcca ggaagagcgc cgtcgccagc tggcggtcgg cgacatagtc
     4441 ctgcgccagc agcccggcta gcgtcgcgtc gatgtccgcc ggcagcgcgc tggcactcgt
     4501 catcgccaca tgaggctagc cggccgcagc aaccgcgcgc tgcgccagca ccgggatcag
     4561 cgccgcgcgg tattcggccg aaccgtgcag atcgctgttg aggtcgccgc tgtcgaccgt
     4621 cacgccgacg caggattcgg gcgc
//
LOCUS       DASUCY010000367         6210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_8110, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000367 DASUCY010000000
VERSION     DASUCY010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 6210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6210
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8110"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VFV25_07305"
     CDS             complement(<1..426)
                     /locus_tag="VFV25_07305"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY1393167.1"
                     /translation="MQQRSILIVEDEAIVAMDLRAQLQKLGYKICGTAGTASEAKSLV
                     ERLKPDLALMDIQLQGPEDGISAAQAICQRHRVPVIFLTAFSDEDTVRRAAGTAAYGY
                     LTKPFQIKELRAGIEIALSKAAMERQLREADLWFVHTLRC"
     gene            589..1944
                     /locus_tag="VFV25_07310"
     CDS             589..1944
                     /locus_tag="VFV25_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEY1393168.1"
                     /translation="MPKLLRNTLLSFRDLLATAGPFMLLVLVLLALAYWLLDPTPPKR
                     VVLATGQPQGAYAEFGQRYVEQLKKFGVKVTLRETQGSAENLKLLEDPNSGVDLAFVQ
                     GGAGRLRQSDDERLSGLVTLGSLFYEPVWLFYREDSAKRLLHKAQLDGLGELKGWRVS
                     IGAPGSGITNLMYSLFEANGMDSKDLTLSREPQTPAVMGLLAGDLDALVFVSAPESLM
                     VQMLLQTPGIRLLDFAQAEAYARRLPILAPVTLPRGVIDLARDLPPDDVHLIAPTAML
                     VARESTHPALQQLFVQAAEAIHSPPGWFSHKDEFPTPRHTVLPVSKEAQRFYDSGPPL
                     LQRYLPFWFANLVDRMWVVLVSLIAVLLPLSRVLPPLYELRIRSRIFRWYGRLRQIES
                     DLVHAEQAPGHLLKELDELDARVERIPVPLSHTDELYALRSHIDMVRQRLRDKAQDRT
                     G"
     gene            1957..3345
                     /locus_tag="VFV25_07315"
     CDS             1957..3345
                     /locus_tag="VFV25_07315"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010467093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY1393169.1"
                     /translation="MNPASPSAGTQRPRVVMIGCGFGGIEAVRTLSGAPVDITLIDRT
                     NHHLFQPLLYQVATAGLAAPSISGPIRHILRREMQRGNLTVLLGEVSDIDVAARCVVL
                     DGRERLPYDHLIVAAGATHSYFGHDAWAPYAPGLKTLADAFEIRRRVLLAFEHAERQA
                     SAEARAPWLTFAVVGAGPTGVEMAGTMAEIARHTLRGEYHRIDTRSARVVLIEGSPRV
                     LTAFPEKLSQRAREQLEQLGVEVLADARVSEISGDGIQYESRAANGSTQSNWLPCRTV
                     VWAAGVAASPLGRSIAAGTGCTLDRAGRVVVEPDLSVPGHPEIQVVGDLAAALSHNKR
                     GQPTPVPGVSPGAKQMGRMAAANLLRRLRGEPTQAFRYVDYGSLATIGRKSAVAMVDV
                     PLLGNVRFSGYPAWLFWLFVHIYFLIGFRNRFVVLLDWAWAYFSFERQARVVAGAEPA
                     IAATKAPEGPNP"
     gene            3411..5228
                     /locus_tag="VFV25_07320"
     CDS             3411..5228
                     /locus_tag="VFV25_07320"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEY1393170.1"
                     /translation="MDTRTSPIRPRLASLRSAMEQHGIAAVLVPSSDPHLSEYLPGHW
                     QGREWFSGFTGSMGTLVVMRERAALFADSRYWVQAEAELMGSGIELVKIPTGNSTAHV
                     EWLASQVPAGQTVAVDGAVLGLAAAQHLRAALERAGVKLSTHLDPLAAVWPERAALPA
                     APIYEHRAPHAPLARSAKLAQVREAMANAGATHHFISTLDDLAWLFNLRGSDVNYNPV
                     FLAHALISADVTTLFVGAGKIDAALKNTLAADGIALQAYGEAAAALAALPAGSTLLID
                     PRRVTLGLREAVSASVKVIEAINPTTLAKSRKSAAEAGFVREAMERDGAAMCAFYAWL
                     EQALGRENITEITIDERLSAERAKQPGCVSLSFNTIAGFNANGAMPHYHATPSSHAAI
                     SRKDGLAAEGLLLIDSGAQYLGGTTDITRVWAIGTPNTAQKRDFTLVLKGMMALSRVS
                     FPRGTLSPMLDAIARTPIWAAGVEYGHGTGHGVGYFLNVHEGPQSISKNLPEPHMAME
                     PGMITSIEPGIYRPGKWGVRIENLVLNVPTQTTEFGEFLGFETLTLCPIDTRCIDRSL
                     MREDEIAWLDAYHDEVRRRLSPLLSGDALRWLEARTQPV"
     gene            5285..5944
                     /locus_tag="VFV25_07325"
     CDS             5285..5944
                     /locus_tag="VFV25_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007839245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEY1393171.1"
                     /translation="MHKAIPADRPQTLGEEIANAVSHGLGFLLAVASLPILVWRAARV
                     GGAADVVGASVFAGTMILLYLVSTLYHALPAGRAKLWLNRLDHAAIYVFIAGSYTPFT
                     LGVLRGSWGWSLFGIVWAIAAVGVAIKLMDRLRHPLLSTALYVAMGWVAVVAVAPLVD
                     RMPTMGLMWLVIGGLSYTLGAAVFLLDNRIRYAHFAWHLLVLGGSVSHFFAALWYATA
                     R"
     gene            complement(5965..>6210)
                     /locus_tag="VFV25_07330"
     CDS             complement(5965..>6210)
                     /locus_tag="VFV25_07330"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY1393172.1"
                     /translation="EMARLSVAQFERQFVRVMQLTPRAWLTSVRLDAAMHALEEGDES
                     IAAIAQASGFADQSAFARTFRKHVGLTPTAYRISRRG"
BASE COUNT          950 a         2206 c         2041 g         1013 t
ORIGIN      
        1 gcagcgcagg gtgtgcacga accacaggtc ggcctcgcgc aactggcgct ccatggctgc
       61 cttggacaga gcgatctcga taccggcccg cagctccttg atctggaagg gcttggtcag
      121 atagccataa gccgccgtgc ctgcagcgcg ccgcactgtg tcctcgtcgc tgaacgcagt
      181 gaggaagatg accggcaccc tgtgccgctg gcagatcgcc tgcgcagcgc tgatgccgtc
      241 ttcgggtcct tgcagctgga tgtccatcag cgcgaggtcc ggcttcagcc gctccaccag
      301 gctcttggcc tcgctggcgg tgccggcggt gccgcagatc ttgtagccca gcttctggag
      361 ctgcgcgcgc aaatccatcg cgacgatggc ctcgtcctcg acaatcaaga tcgagcgctg
      421 ctgcatgaga cctcccttcg ctaccacgcg agacagcaat gcatcccggc ggtcaggcgt
      481 tttgtgcggc acattttgac gaatttgcga cgttgttgcc aacttccccc aaagggggat
      541 gtcctcgaaa atgcctcccc gcagcacgcg ccacttttgc ttcgaaccat gcccaagctc
      601 ttgcgcaaca ccttgctgtc cttccgcgac ctgctcgcca cggccgggcc cttcatgctg
      661 ctggtgctgg tgctgttggc gctggcctac tggctgctcg acccgacacc gcccaaacgc
      721 gtcgtgctcg ccaccggcca gccgcagggc gcctatgcgg aattcggcca gcgctatgtc
      781 gagcaactga agaagttcgg cgtcaaggtc acgctgcgcg agacgcaggg ctcggccgag
      841 aacctgaagc tgctggagga tccgaactcc ggcgtggacc tggccttcgt gcagggcggc
      901 gcgggccggc tgcgacagtc cgacgacgag cgcctaagcg gcctcgtaac gctgggcagc
      961 ctgttctacg agccggtgtg gttgttctac cgcgaggatt cggccaagcg gctgttgcac
     1021 aaggctcagc tcgacggcct gggcgagctc aagggctggc gcgtcagcat cggcgcgccg
     1081 ggcagcggca tcaccaatct gatgtacagc ctcttcgagg ccaacggcat ggacagcaag
     1141 gacctcacgc tgtcgcgcga gccgcagacg cctgccgtga tgggcctgct ggccggagat
     1201 ctggacgcat tggtgttcgt ctcggcgccc gagtcgctga tggtgcagat gctgttgcag
     1261 acgcccggca tacgtctgct cgacttcgcg caagccgagg cttatgcgcg ccgcctgccc
     1321 atcctggcgc cggtcacgct gccgcgcggc gtgatcgacc ttgcgcgcga cctgccgccg
     1381 gacgacgtgc atctgatcgc ccccacggcg atgctggtgg cgcgcgagtc gacccacccg
     1441 gccctgcagc agctgttcgt gcaggcggcc gaggcgatcc acagcccgcc gggctggttc
     1501 tcgcacaagg acgagttccc cacgccgcgt cacacggtgc tgccggtgtc caaggaggcg
     1561 cagcgcttct acgattccgg accgccgctg ctgcagcgct acttgccctt ctggttcgcc
     1621 aacctggtcg accgcatgtg ggtggtgctg gtgtcgctga tcgcggtgct gctgccgctg
     1681 tcgcgcgtgc tgccgccgct gtacgagctg cgcatacgct cacgcatctt ccgctggtac
     1741 ggccggctgc gccagatcga gtcggacctg gtgcatgcag agcaagctcc cggtcacctg
     1801 ctgaaggagc tagacgagct ggacgccagg gtcgagcgca tccccgtgcc gttgtcgcac
     1861 accgacgagc tctatgcgct gcgcagccac atcgacatgg tgaggcagag gctacgcgac
     1921 aaggcacagg accggactgg ctgagacaat ccttccatga acccagcatc gccctccgcc
     1981 ggcacccagc gcccacgcgt cgtcatgatc ggctgcggct tcggcggcat cgaggcggtg
     2041 cgtaccctgt ccggcgcgcc ggtcgacatc acgctgatcg accgcaccaa ccatcatctg
     2101 ttccagcccc tgctttacca ggtggccacg gccggcctgg cggcgccgtc gatctcgggg
     2161 ccgatacgcc atatcctgcg gcgcgagatg cagcgcggca acctcacggt gctgctgggc
     2221 gaagtgagcg acatcgacgt cgcggcgcgc tgcgtggtgc tggacggccg cgaacgcctg
     2281 ccctacgacc atctcatcgt cgcggccggc gccacgcaca gctatttcgg ccatgacgcc
     2341 tgggcgcctt acgcccccgg gctcaagacc ctggccgacg ccttcgagat ccgccgccgc
     2401 gtgctgctcg ccttcgagca tgccgagcgc caggccagcg ccgaggcgcg cgcgccctgg
     2461 ctcaccttcg cggtggtggg ggccggcccc accggcgtgg agatggccgg caccatggcc
     2521 gagatcgcgc gccacacgct gcgcggcgaa taccatcgca tcgacacgcg cagcgcccgc
     2581 gtcgtgctga tcgagggctc gccgcgcgtg ctgacggcct tcccagagaa gctctcgcag
     2641 cgtgcgcgcg agcagctcga acagctgggc gtcgaggtct tggccgatgc acgcgtgagc
     2701 gaaatcagcg gcgacggcat tcaatacgag agccgcgcag caaacggaag cacgcagtcc
     2761 aactggctgc cctgtcgcac cgtcgtgtgg gcggccggtg tcgccgcctc gccgctcggg
     2821 cgcagcatcg ccgcgggcac cggctgcacg ctggaccgcg caggccgcgt cgtcgtcgag
     2881 cccgacctga gcgtgcccgg ccatcccgag atccaggtcg tcggcgacct ggccgcggcg
     2941 ctgagccaca acaagcgcgg ccagcccacg ccggtgcccg gcgtcagccc cggcgccaag
     3001 cagatgggcc gcatggccgc cgccaatctg ctgcgccgcc tgcgcggcga gccgacgcag
     3061 gccttccgct acgtcgacta cggctcgctc gccaccatag gccgcaagtc cgcggttgcg
     3121 atggtcgatg tgccgctgct cggcaacgtg cgcttcagcg gctacccggc ctggctgttc
     3181 tggctcttcg tgcacatcta tttcctgatt ggcttccgca accgcttcgt ggtgctgctc
     3241 gactgggcct gggcctattt cagcttcgag cgccaggcca gggtggtcgc cggggccgag
     3301 ccggccattg cagccaccaa ggcgcctgaa gggcccaacc cctagaatcc ccatcacccc
     3361 gcgccaaggc cccacccggg ccgccacgcg cctcaacacg gacccatacg atggacaccc
     3421 gcacttcccc gatccgcccc cgcctggcct cgctgcgctc cgcgatggag caacacggca
     3481 tcgctgccgt gctcgttcct tccagcgacc cgcacctgtc cgaatacctg cccggccatt
     3541 ggcaggggcg cgaatggttc tcgggcttca ccggctcgat gggcaccctg gtcgtgatgc
     3601 gcgagcgcgc cgccctcttc gctgacagcc gctattgggt gcaggccgaa gccgaactga
     3661 tgggcagcgg catcgaactc gtcaagatcc cgaccggcaa ttccacggcc catgtcgaat
     3721 ggttggcatc ccaggtgccc gcgggccaga ccgtcgcggt cgacggcgcc gtgctgggcc
     3781 tggccgccgc gcagcatctg cgcgccgcgc tggagcgcgc cggcgtcaag ctgagcactc
     3841 atctcgatcc gctggccgcg gtctggcccg agcgggccgc gctgcccgcc gcgccgatct
     3901 acgagcaccg cgcgccgcat gcgccgctgg cacgcagcgc caagctggct caagtgcgcg
     3961 aagcgatggc gaacgcaggc gcgacgcacc atttcatttc cacgctcgac gacctggcct
     4021 ggctcttcaa tctgcgcggc tcggacgtca actacaaccc ggtcttcctg gcccatgcgc
     4081 tgatctcggc cgatgtcacc acgctcttcg tcggcgccgg caagatcgac gcggccctga
     4141 agaacaccct ggcggccgac ggcatcgcgt tgcaggccta tggtgaggca gcagctgcgc
     4201 tggcggccct gcccgcaggc agcacgctgc tgatcgaccc gcgccgcgtc acgctgggcc
     4261 tgcgcgaggc ggtgtcggcg agcgtgaagg tgatcgaggc gatcaacccg acgacgctgg
     4321 ccaagagccg caagagcgcc gcggaggccg gcttcgtacg cgaggccatg gagcgcgacg
     4381 gtgccgcgat gtgcgcgttc tacgcctggc tggagcaggc gctgggccgc gagaacatca
     4441 ccgagatcac gatcgacgaa cgcctgagcg ccgagcgcgc gaagcagccg ggctgtgtct
     4501 cgctgagctt caacaccatc gccggcttca acgccaacgg cgcgatgccg cattaccacg
     4561 cgacgccctc gtcgcacgcc gccatctcgc gcaaggacgg cctcgccgcc gagggcctgc
     4621 tgctgatcga ttcgggcgcg caatacctgg gcggcacgac cgacatcacg cgcgtctggg
     4681 ccatcggcac gcccaatacc gcacagaagc gcgacttcac attagtgctc aagggcatga
     4741 tggcgctgtc gcgcgtgagc ttcccacgcg gcacgctgtc gcccatgctg gacgcgatcg
     4801 cgcgcacgcc gatctgggcc gccggcgtcg aatacggcca cggcacgggc cacggcgtag
     4861 gctacttcct caacgtccac gaaggaccgc agagcatttc caagaacctg cccgagcccc
     4921 acatggcgat ggagcccggc atgatcacct cgatcgagcc cggcatctac cgacccggca
     4981 aatggggcgt gcgcatcgag aacctggtgc tcaacgtgcc gacgcagacc accgagttcg
     5041 gcgagttcct cggcttcgag acgctcacgc tgtgcccgat cgatacgcgc tgcatcgatc
     5101 gcagcctgat gcgcgaagac gagatcgcct ggctcgacgc ctatcacgac gaagtgcgcc
     5161 gccgcctgag cccgctgctg agcggcgacg cgctgcgctg gctggaggcg cgcacgcagc
     5221 cggtgtgagc gcaacctgga agccggcttc ctgcgctgcg ccggtggcac ggcacaatgt
     5281 gccgatgcac aaggccatcc ccgctgaccg cccccagacc ttgggcgagg aaatcgccaa
     5341 cgcggtcagt cacggactgg gcttcctgct ggccgtggcc tcgctgccca ttctggtctg
     5401 gcgggccgct cgcgtgggtg gggcggccga cgtcgtggga gccagcgtat tcgccggcac
     5461 gatgatcttg ctgtatctcg tctcgacgct gtaccacgcg ctgccggcgg gtcgcgccaa
     5521 gctttggctg aatcgcctgg atcacgcggc gatctacgtg ttcattgccg gcagctacac
     5581 gccgttcact ctgggggtgc tgcgcggtag ctgggggtgg tcgctgttcg gcatcgtgtg
     5641 ggcgatcgca gccgtgggcg tggcgatcaa gctgatggac cgcctgcggc atccgctgct
     5701 gtccaccgcc ttgtatgtgg caatgggttg ggtggccgtt gttgccgtcg cgccgctggt
     5761 cgaccgcatg cccacgatgg ggcttatgtg gctcgtgatc ggcggcctga gctacacgct
     5821 gggggcggcg gtgttcctgc tcgacaaccg catccgctat gcgcacttcg cgtggcacct
     5881 gctcgtgctt ggcggcagcg tgagccattt cttcgccgcg ctttggtacg ccactgcgcg
     5941 gtaaggccgg caagccgcgc tgggtcagcc ccggcgtgag atgcgatagg ccgtcggcgt
     6001 cagaccgacg tgcttgcgga aagtgcgcgc gaacgcgctc tggtcggcaa agccgctggc
     6061 ctgcgcgatc gcagcgatgg actcgtcgcc ttcttcgagg gcatgcatcg ccgcatccag
     6121 ccgcaccgag gtcagccacg cacgcggcgt gagctgcatc acccgcacga actggcgctc
     6181 gaactgcgcg accgacaggc gcgccatctc
//
LOCUS       DASUCY010000368         2653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_345198, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000368 DASUCY010000000
VERSION     DASUCY010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345198"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VFV25_07335"
     CDS             <1..447
                     /locus_tag="VFV25_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY1393173.1"
                     /translation="GGHAISRGFNRQVYTRMFLRTMKPKALAKLAQHPGLFDRAKLLA
                     ASDLHDFDDVFTAPLHGFRDVDDYWHRASAKPQLPRIRVPALVLNALNDPFVPRRSLP
                     RPDEVGAHVTLWQPAYGGHVGFPLGRFPGHVLGLPWGVMAWLGSRF"
     gene            554..1672
                     /gene="pstS"
                     /locus_tag="VFV25_07340"
     CDS             554..1672
                     /gene="pstS"
                     /locus_tag="VFV25_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEY1393174.1"
                     /translation="MKFAVSPFLASLAAMTALAGGLLLSRPAQAGNEVTLNGTGATFP
                     AKVYAQWAESYAKERGIVVHYEGTGSSMGQKQIKARAVDFGATDVPMSSAELEKNGLF
                     QFPTLVGGVVPVVHLPGVAAGRLHLNAEVLAGIFTGHIQRWNDPAIAALNPGLALPEQ
                     HITRVVRADGSGTTEVFVSYLKQVAPGQAADIVGQGTTVQWPGSVNAVEGSGKVAAAV
                     KATPGAIGYISSDYVLRENLTPASLRNKRGDWVNPSIESYRAAVRAGGLFRDSLEAAP
                     LLDVDGPGVWPIVTATYIVVDRSPASLERAGRTLNFFYRSFLMGDRAVAGSGFAPLPT
                     ETQARIVAKLSSFRTQDGRVLPVMGGLDGPATTIAAQH"
     gene            1700..2251
                     /locus_tag="VFV25_07345"
     CDS             1700..2251
                     /locus_tag="VFV25_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HEY1393175.1"
                     /translation="MDEIVRAALKKWPNVPACYGWLALDARGDWYMRDDRVQAAGPFP
                     QVKGSRINHDKLKEFIARNYEADAASGAWFFQNGPQRVYVELEAAPLVLGVAVETQDG
                     RASWCVQTHTGRDAGRVKGAYSDESGRLFLDTEAGFGIVRSLDMDAAADAVMAGLWTP
                     QTVSFDSLPQRHGYCLSPARLQP"
     gene            complement(2322..>2653)
                     /locus_tag="VFV25_07350"
     CDS             complement(2322..>2653)
                     /locus_tag="VFV25_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008352512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY1393176.1"
                     /translation="QTAAAAPVAATGAAAALAAINAAPAKNEAKADAGQALYDQTCQA
                     CHAAGLAGAPKFGDKAAWAPRIAQGLNTLVDHAIHGKNAMPPRGGSTASDADIKLAVS
                     YMVNAAK"
BASE COUNT          383 a          931 c          897 g          442 t
ORIGIN      
        1 ggcgggcacg cgatctcgcg cggcttcaac cggcaggtgt acacgcgcat gttcctgcgc
       61 acgatgaagc ccaaggcgct ggccaagctg gcccagcatc cgggcttgtt cgatcgcgcc
      121 aagctgctgg cggcgagcga cctgcacgac ttcgacgatg tcttcaccgc gccgctgcat
      181 ggctttcgcg atgtcgacga ctactggcac cgtgcctcgg ccaagcccca gctgccgcgc
      241 atccgcgtgc cggcgctggt gctgaatgcc ctcaacgatc ccttcgtgcc gcgccgcagc
      301 ctgccgcgcc cggatgaagt gggcgcccac gtgacgttat ggcagccagc ctatggcggg
      361 catgtcggct ttcccctggg gcgctttccc ggccacgtgc tgggcctgcc ctggggcgtg
      421 atggcctggc tgggcagccg gttttgaaac gattgcttgc ctcgaccggc gcctgagcgt
      481 gaaaccttgc ctgggcgctg cgaacctcgc tccctagaat tgcggggttt tactgagttg
      541 ttggctgtac cccatgaagt tcgctgtgtc gccctttctc gcttccctgg ctgcgatgac
      601 cgccctggcc ggtggcctcc tgctgagccg cccggcccag gccggcaatg aggtcacgct
      661 caacggcacc ggcgccacct tccccgccaa ggtctatgcc cagtgggccg agtcctatgc
      721 caaggagcgc ggcatcgtcg tgcactacga gggcaccggc tcttcgatgg gtcagaagca
      781 gatcaaggcc cgcgcggtcg atttcggcgc taccgatgtg ccgatgtcca gcgccgagct
      841 cgagaagaac ggcctgttcc agttcccgac cctggtcgga ggcgtggtgc cggtggtgca
      901 tctgcccggc gtcgcggccg gccgcctgca cctgaacgcc gaggtgctgg cgggcatctt
      961 caccggccac atccagcgct ggaacgatcc cgcgatcgcg gccctgaacc ccgggctggc
     1021 gctgcccgag cagcacatca cccgcgtggt gcgcgccgac ggctccggca ccaccgaagt
     1081 cttcgtcagc tatctgaagc aggttgcgcc cgggcaggcc gccgacatcg tcggccaggg
     1141 cacgacggtg cagtggccag gcagcgtgaa tgccgtggag ggctctggca aggtcgccgc
     1201 cgccgtgaag gccactcccg gcgcgatcgg ctacatctct tcggactatg tgctgcgcga
     1261 gaacctgacg cccgcctcgc tgcgcaacaa gcgcggcgat tgggtcaacc ccagcatcga
     1321 gagctatcgc gctgccgtca gggcgggcgg tctgttccgc gacagcctcg aggccgcgcc
     1381 cctgctcgac gtggacggcc ccggcgtctg gcccatcgtc accgcgacct acatcgtggt
     1441 ggaccgctcg cccgcctcgc tggaacgcgc cggccgcacc ctcaacttct tctatcgctc
     1501 cttcctgatg ggcgatcgcg ccgtcgccgg cagcggcttc gcgcccctgc cgaccgagac
     1561 ccaggcgcgc atcgtcgcca agctgtccag cttccgcacc caggacggcc gcgtcctgcc
     1621 cgtgatgggc ggcttggatg gccctgcgac caccattgcc gcgcaacact gatcgcgccg
     1681 ggagtccaca atccgcggca tggatgagat cgtccgtgcc gcgctgaaga aatggcccaa
     1741 tgtgcccgcc tgctacggct ggctcgccct cgatgcgcgc ggcgactggt acatgcgcga
     1801 cgaccgggtg caggcggccg ggcccttccc gcaggtgaag ggcagccgca tcaatcacga
     1861 caagctcaag gaattcatcg cgcgcaacta cgaggccgat gcagccagcg gcgcgtggtt
     1921 ctttcagaac ggcccacaac gcgtctacgt ggagctggag gcggcgccgc tggtgctcgg
     1981 cgtcgctgtc gagacgcagg acggccgcgc cagctggtgc gtgcagaccc acaccggccg
     2041 cgacgccggt cgcgtgaagg gcgcctacag cgatgaaagc ggcaggctct tcctcgacac
     2101 cgaggccggc ttcggcatcg tgcgctcgct ggacatggat gccgcggccg atgccgtgat
     2161 ggcagggctg tggacaccgc agacggtgag cttcgacagc ttgccgcagc gccacggcta
     2221 ttgcctgagc cctgcaaggc tacagccctg agtggcgttt tgcgctcgcc aaaagaaaac
     2281 cggccccaag gggccggttc tgcttggcag gccgcagccg attacttggc ggcgttgacc
     2341 atgtagctca cggccagctt gatgtcggcg tccgaggccg tcgagccgcc gcgcggcggc
     2401 atggcgttct tgccatggat cgcgtgatcg accagcgtgt tcagaccttg cgcgatgcgc
     2461 ggtgcccagg ccgccttatc gccgaacttg ggcgcgccgg cgagaccggc cgcatggcag
     2521 gcctggcagg tctggtcgta cagggcctgg ccggcatcgg ccttggcttc gttcttggcc
     2581 ggcgcggcat tgatcgcggc gagggccgca gcggcgccgg tggcggccac gggcgcggcg
     2641 gctgccgtct gcg
//
LOCUS       DASUCY010000369        11674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_152589, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000369 DASUCY010000000
VERSION     DASUCY010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 11674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11674
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152589"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /gene="secA"
                     /locus_tag="VFV25_07355"
     CDS             complement(<1..392)
                     /gene="secA"
                     /locus_tag="VFV25_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804245.1"
                     /note="functions in protein export; can interact with
                     acidic membrane phospholipids and the SecYEG protein
                     complex; binds to preproteins; binds to ATP and undergoes
                     a conformational change to promote membrane insertion of
                     SecA/bound preprotein; ATP hydrolysis appears to drive
                     release of the preprotein from SecA and deinsertion of
                     SecA from the membrane; additional proteins SecD/F/YajC
                     aid SecA recycling; exists in an equilibrium between
                     monomers and dimers; may possibly form higher order
                     oligomers; proteins in this cluster correspond SecA1;
                     SecA2 is not essential and seems to play a role in
                     secretion of a subset of proteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEY1393177.1"
                     /translation="MSLKLLTQIFGSRNDRLLKQYRRVVERINALEPQFEKLSDDELR
                     GQTQHFKDRLAKGETIDALLPEAFAVVREGGKRVLKMRHFDVQLIGGMVLHNGKIAEM
                     RTGEGKTLVATLPVYLNALTGRGVHVVTV"
     gene            complement(649..1641)
                     /locus_tag="VFV25_07360"
     CDS             complement(649..1641)
                     /locus_tag="VFV25_07360"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEY1393178.1"
                     /translation="MQVIITHGTFARSRVLQFSRLQLILVAMLLSFLLVLLSGAIYHF
                     IFLKAAREGWPVVGQVLRLVERDELAQRDRYMRANLDAMAQKVGEMQAKLVRLDAMGE
                     RVSSLAGLKPEELRALQSATSPASSPAAGKQLDGGKGGPYVPIASPSLEQLNQIVSAL
                     DEQADQGSDLFTLAESRLMESRLHSLMVPSSAPVEGPVGSGFGFRSDPFTGHTALHTG
                     LDFPAEVGTPIMAAAGGVVIAAEVHPQYGQMVELDHGNGLVTRYAHASRLLVKQGDII
                     KRGQRIALVGTTGRSTGPHLHFEVLVEGVPQNPAKFLAGRGGAGTALARAASAS"
     gene            1660..1998
                     /locus_tag="VFV25_07365"
     CDS             1660..1998
                     /locus_tag="VFV25_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393179.1"
                     /translation="MKSTTTTQRSVPDALPLRDALERSAPLERLRQRIADSNARLAAL
                     LPCLPQALLPHVSAGPVDEAAWTLLARNAAVAAKLRQLQPRLEAALRQQGWEVSLIRI
                     RVQPGEATKM"
     gene            complement(2003..2078)
                     /locus_tag="VFV25_07370"
     tRNA            complement(2003..2078)
                     /locus_tag="VFV25_07370"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2043..2045),aa:Thr,seq:tgt)
     gene            complement(2171..2698)
                     /locus_tag="VFV25_07375"
     CDS             complement(2171..2698)
                     /locus_tag="VFV25_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HEY1393180.1"
                     /translation="MSTPPSAPEAQGTSTRPYLIRALHEWCTDNGFTPYLAVFVDSTV
                     RVPQEYVKNNEIVLNIGFEATSGLQLGNEVVSFKARFGGVARDIVVPVDHVIAIYARE
                     NGQGMAFPVPGETAAAGEESAHEDSGTGAPAEPGKGLRLAVPPAEAPPAAEATPEPEP
                     PAPPGGPRPSLKRVK"
     gene            complement(2729..3340)
                     /locus_tag="VFV25_07380"
     CDS             complement(2729..3340)
                     /locus_tag="VFV25_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006999756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEY1393181.1"
                     /translation="MMVLYSGTTDPYSHRCRFVLFEKGMDFEIRDVDLFAKPEDIALM
                     NPYNEVPILVERDLILYESHIINEYIDERFPHPQLMPGDPVARARVRLFLFNFEKELF
                     SNVNLLEGRGVKATDKQLEKARGQIRDRLTQLAPIFLKNKYMLGDDFSMLDVAIAPLL
                     WRLDYYGIDLSKNALPLLKYAERIFSRPAYIEALTPSEKVMRK"
     gene            complement(3462..4229)
                     /locus_tag="VFV25_07385"
     CDS             complement(3462..4229)
                     /locus_tag="VFV25_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEY1393182.1"
                     /translation="MKKLIAILLASLALMSAARASEGGMELDKFPADKLTDVAALQRG
                     AKMFVNYCLNCHAAAFMRYNRLKDIGLTDVQIKNNLLFATDKVGETMKIALDPRQAKE
                     WFGATPPDLTVIARSRGGRGGSGADYLYTYLRSYYRDDTRPTGWNNLAFHNVGMPNVL
                     WELQGQRAAKFVEAADNGEEAKGEPRFAGFEQLTPGKLTPQEFDSAVGDLVAYLQWMG
                     EPAQGQRERVGAVVLVFLFIFSLIAWRLNAAYWKDVK"
     gene            complement(4252..5652)
                     /locus_tag="VFV25_07390"
     CDS             complement(4252..5652)
                     /locus_tag="VFV25_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEY1393183.1"
                     /translation="MAEFKEISPDAPAAMKLLNWVDNRFPASKLWKEHLSEYYAPKNF
                     NFWYFFGSLALLVLVIQIVTGIFLVMNYKPDASLAFASVEYIMRDVPWGWLIRYMHST
                     GASAFFIVVYLHMFRGLMYGSYRKPRELVWIFGCAIFLCLMAEAFMGYLLPWGQMSYW
                     GAQVIVNLFSAIPLIGNELSLMIRGDYVVSDATLNRFFSFHVIAVPLVLLGLVVAHVI
                     ALHEVGSNNPDGIEIKEKKDARGIPLDGIPFHPYYSVHDLMGVSVFLFIFSLILFFVP
                     EGGGYLLEYNNFIPADPLKTPPHIAPVWYFTPFYSMLRATTDQMVYVLIALLVVAVVL
                     ALLRGGLSSTGKLAVAIGGLVGVAALWSFEAKFWGVVVMGGAVIILFFLPWLDRSPVK
                     SIRYRPDWHKYVYAVFVAVFIVLGYLGIQPPSPVGGYVSMIGTLLYFGFFLLMPWWSR
                     LGSFKPVPERVTYSAH"
     gene            complement(5676..6290)
                     /gene="petA"
                     /locus_tag="VFV25_07395"
     CDS             complement(5676..6290)
                     /gene="petA"
                     /locus_tag="VFV25_07395"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEY1393184.1"
                     /translation="MSDNKGESQVDKGRRTWVAIACAAGGVGGVATAVPFVSTFEPSE
                     RARAAGAPVEADISALKPGEMMTVEWRGKPVWIVKRTPEMLASLKKTDGLVADPQSER
                     KPDDITPMKDTRNEWRSIKPEIFVCVGICTHLGCSPSAKFAPGAQPSLPDDWAGGFLC
                     PCHGSTFDLAGRVFKNKPAPDNLEVPPYTFLSDNKLLIGEAKKA"
     gene            6524..6937
                     /gene="mscL"
                     /locus_tag="VFV25_07400"
     CDS             6524..6937
                     /gene="mscL"
                     /locus_tag="VFV25_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEY1393185.1"
                     /translation="MGFMTEFKEFALKGNVMDLAVGVIIGGAFGKIVDSVVNDLIMPL
                     VGRVTGGLDFSSYYIALAGQAPGLPLADAKKAGAVFAYGSFLTVLVYFVILAFIIFMM
                     VKQMNRLKKEAPAPAPAATPEDVLLLREIRDSLKK"
     gene            7117..9708
                     /locus_tag="VFV25_07405"
     CDS             7117..9708
                     /locus_tag="VFV25_07405"
                     /inference="COORDINATES: protein motif:HMM:NF019410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HEY1393186.1"
                     /translation="MTDSRLQPALQTAIARIQATAQAVADQVADALGLAAVSAASNTQ
                     RKAYMTAQFDLRRKQAAFNLTFGNVLRDKVMQEALPGVRGQHSLAATDWASLSLVDDA
                     EVEAQVSAERLALEVEHECEWELRELAAYTGSLLGLGHADQARNPLRPEMIGKALFRG
                     IETVSDQPELRKLLARELSRSLAQAMRACYQSIVEDLRRRGVQPVGLNVKQVEGPGNE
                     MAREVPRETQSGYQSAVKRSEAEAAKALSAMFGMAMPQGLHDLGTHAGSGPGSLSGMT
                     MPDPASLLRTGASTRGGASGLGSGYGDLGSQRGAPARGSDAQLTEVIRRLAFLTSGYG
                     DLGGEAPTKPASLSGGALRPGAPERRFTVTGGGGYGSSGHGGLSSGHHLQGGVTGYDA
                     LTGLMAANLIHQHREELRRASTGALDHMVIDVVGALFDQVLSDPKVAPAMARQIARLQ
                     LPVLRAALKDVGFFSSRRHPVRRFVNRIASLAAAYDDFDEGLGKDFLDKVRSLVQQIV
                     EGDFDQMELYDSKLAELESLIQQQTAREAPQHAQVAALLDDKETELRIQQRYMRELQA
                     HLAPLALHEFLREFLSQVWSQVQVLAVKREGPDSALAKRVNALPRELVFSVQPKGTPQ
                     LRKEFLLKLPQLMKDLNEGLALIRWPEAAKKDFLAKLLPAHAESLKGQPLTDFEQRQL
                     SHQLAQVEKVAIPKVQDVARDSSILPLAADDAIEPSFSPEEMKQVGLVEESAIDWDGK
                     IDIDLDQAADSTPAELDVNLDSPAPPSSGIQLMHHVQPGIAYRMHLDKGWQKVRLNWA
                     SPGRAFFVFSYGRKHPKTLSLSARMLARMCETGRFKAFEQTELIERATARARKQLAEL
                     TAAASKH"
     gene            complement(9807..10844)
                     /gene="pdxA"
                     /locus_tag="VFV25_07410"
     CDS             complement(9807..10844)
                     /gene="pdxA"
                     /locus_tag="VFV25_07410"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEY1393187.1"
                     /translation="MQTKSTELFPLALTMGDACGIGPEILAMAFRRGAVSSGFVLGDV
                     GVMRRAAQLTGGLLPVVEIEVPADVPACPPDCLPVLQVEGLPGDLLQAPLGRVDARAG
                     AAAAACIRRAVALAMRGEVGAVVTAPIHKEAFAAAGVSYPGHTEMLQALSGAQAVRMM
                     LANEELKTVLVSIHVSLRRAIECVTREAVLETLRIAHRSAALWGQPRPRIAVAGLNPH
                     AGEGGLFGDEELHAIAPAIADARAEGIAASGPYAPDTVFMRARSAPGHPGEFDLVVAQ
                     YHDQGLIPVKYLGVEQGVNVTLGLPFVRTSPDHGTAFDLAGTGRADPASLIAALQMAR
                     RLAGGRRVEMS"
     gene            11021..11548
                     /locus_tag="VFV25_07415"
     CDS             11021..11548
                     /locus_tag="VFV25_07415"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HEY1393188.1"
                     /translation="MNQKLAKKTFVALAFAAAALPGFAQNAYVGGNIGSPDWKTGSIS
                     GISGDSKGTAYKLYGGYSFNPNFALEGGWADLGKLSGNAGATTGRGLFVDAVGRLPLA
                     NDFSAIGRVGVFNGKLSNPVNSGTGTDFKYGLGVQYDLSKNMALRGEWERYRFNALNI
                     KPNVDSLSVGLTYGF"
BASE COUNT         2012 a         3911 c         3885 g         1866 t
ORIGIN      
        1 accgtcacga catgcacgcc cctgccggtc agcgcattga ggtagaccgg cagcgtggcc
       61 accagggtct tgccctcgcc ggtgcgcatc tcggcgatct tgccgttgtg cagcaccatg
      121 ccgccgatca gctgcacgtc gaagtggcgc atcttgagca cgcgcttgcc gccctcgcgc
      181 accacggcga aggcctcggg cagcagggca tcgatcgtct cgcccttggc gaggcgatcc
      241 ttgaagtgct gggtttggcc gcgcagctca tcgtcgctga gcttctcgaa ctgcggctcc
      301 agtgcgttga tcctctcgac cacgcgccgg tactgcttca gcaggcggtc gttgcggctg
      361 ccgaaaatct gggtgagaag cttgagcgac atgcgagtcc gaagatgggg aacgaggccg
      421 cgcggggttc cggcgcgggc gggtttgcgg tgtcatcgct agacgcagcc atgcagcttg
      481 cgatgacacc gcaagccacc ccagctgggg tcttgccggc gccttgcaag cccggcgcag
      541 gaggcatcca cccgcgccgg ctgccggaaa cggccgccgc aaaaggcaaa aattctagca
      601 tcggccccac cggggagccg ggcttgccca ggcagaccgg tggccgcgtc aggacgccga
      661 agcggcgcgg gccaaggcgg tgccggcgcc gcctcggccg gccaggaact tggccggatt
      721 ctgaggcacg ccttcgacca gcacctcgaa atgcagatgc ggtccggtgg agcgcccggt
      781 ggtgcccacc agggcgatgc gctggccgcg cttgatgatg tcgccctgct tgacgagcag
      841 cctggatgca tgggcgtagc gcgtgaccag cccgttgccg tggtcgagct cgaccatctg
      901 cccgtactgc ggatgcactt cggcggcgat caccacgccg ccggcagcgg ccatgatggg
      961 cgtaccgact tcggccggga agtccagccc cgtgtgcagg gccgtatggc ctgtgaaggg
     1021 gtcgctgcgg aagccgaagc ccgagccgac cggcccctcc accggggccg agctgggcac
     1081 catcagcgaa tgcaggcggc tctccatcag gcgcgactcg gccagcgtga agagatcgct
     1141 gccctggtcg gcctgttcgt ccagcgccga gacgatctgg ttgagctgct ccagcgaggg
     1201 cgaagcaatg ggcacatagg ggccgccttt gccgccatcc agctgcttgc cggcagccgg
     1261 cgagctggca gggctggtcg ccgattgcag ggcccgcagc tcctctggct tgaggccggc
     1321 caggctggag acgcgctcgc ccattgcatc gagcctcacc agcttggcct gcatttcgcc
     1381 gaccttctgg gccatggcat cgagattggc gcgcatgtag cggtcgcgct gcgcgagctc
     1441 gtcgcgctcg acaagacgca gcacctggcc gacgacgggc cagccctcgc gggccgcctt
     1501 caggaagatg aagtggtaga tcgcccccga cagcagcacg agcaggaagc tcagcagcat
     1561 ggcgacgagg atcagttgca ggcggctgaa ctgcagcaca cggctgcgcg cgaaggtgcc
     1621 gtgggtgatg atgacctgca tcgctctaca ctcctcacga tgaaatcgac aacgacgacg
     1681 caacgctcgg tgcccgacgc actgcccctg cgcgacgcac tcgaacgctc ggcacccctt
     1741 gagcgcttgc gacagcgcat tgccgattcc aacgctcgcc tggccgctct cctgccctgc
     1801 ttaccgcagg ccttgttgcc ccacgtgagt gcgggcccgg tggatgaagc tgcctggacg
     1861 ctgctggccc gcaacgccgc cgtggcggcc aagttgcggc agctgcagcc ccggctcgaa
     1921 gctgcactac gccagcaggg gtgggaggtt agcctgatcc gcatccgagt tcaaccgggc
     1981 gaagcgacca agatgtaagc cttggtgccg gctatctgac tcgaacagat gacctaccgc
     2041 ttacaaggcg gttgctctac caactgagct aagccggcga ttcgggaaaa ctgcacgcag
     2101 tttgcgtgcg gctagggcat tttaggtccg ggccccgttg gcccggcagc aaggcggcga
     2161 ctacgcctcg ttacttcacg cgcttcagcg aggggcgcgg cccgcccggc ggcgcgggcg
     2221 gttcgggttc gggcgtggct tcagcggccg gtggcgcctc ggcgggcggc accgcgaggc
     2281 gcagcccctt gccgggctcg gccggcgcgc cggtgccgct gtcctcgtgc gccgactcct
     2341 cgcccgcggc ggccgtttcc ccgggcaccg ggaaggccat gccctggcca ttctcgcggg
     2401 cgtagatggc gatcacatgg tccaccggca cgacgatgtc gcgcgccacg ccgccgaagc
     2461 gggccttgaa gctcaccact tcgttgccca gctgcagtcc gctcgtcgcc tcgaagccga
     2521 tgttgagcac gatctcgttg ttcttcacgt actcctgcgg cacccgcacc gtgctgtcga
     2581 cgaagaccgc gagatagggc gtgaagccgt tgtccgtgca ccactcgtgc agggcacgga
     2641 tcaggtaggg ccgcgtggac gtgccttgag cttcgggggc ggaggggggg gtgctcatcg
     2701 catcgagtcg ggagccttag ccggcgcctt acttgcgcat caccttctcg gacggcgtca
     2761 gcgcttcgat gtaggccggg cgcgagaaga tgcgctcagc gtacttgagc agcggaagcg
     2821 cgttcttgct gaggtcgatg ccgtagtagt cgaggcgcca cagcagcggc gcgatggcca
     2881 cgtcgagcat cgagaagtcg tcgcccagca tgtatttgtt cttcagaaag atgggcgcca
     2941 gctgtgtcag gcggtcgcgg atctggccgc gcgccttctc gagctgcttg tcggtggcct
     3001 tgacgccgcg cccttcgagt agattcacgt tcgagaacag ctccttctcg aagttgaaca
     3061 ggaacagccg cacgcgcgcg cgcgccaccg ggtcacccgg catcaactgc gggtgcggga
     3121 aacgctcgtc gatgtactcg ttgatgatgt gcgactcgta caggatgaga tcgcgctcca
     3181 ccaggatggg cacctcgttg tacgggttca tcagcgcgat gtcttccggc ttggcgaaca
     3241 ggtcgacgtc gcggatctcg aagtccatgc ccttttcgaa cagcacgaag cggcagcggt
     3301 gggaataggg atcggtggtc ccggagtaaa gcaccatcat ggcggcgggc tcccagctag
     3361 agaaacaaga atcgaaaacg caaacggagt gggcggcgca gcctttccca ctccgcgggg
     3421 cgccgcaacc gtagtgacga ctgcggcggc gaacactcgg tttacttcac gtccttccaa
     3481 taggccgcgt tgagtcgcca cgcgatcaga ctgaagatga acaggaagac cagcacgacg
     3541 gcgccgacgc gctcgcgctg gccctgggcc ggctcgccca tccactgcag ataggccacc
     3601 aggtcaccga cggccgaatc gaactcctgc ggcgtcagct tgcccggggt cagctgctcg
     3661 aagccggcga agcgcggctc gcccttggct tcttcgccat tgtccgccgc ttcgacgaac
     3721 ttggcggcgc gctggccctg cagctcccac agcacattgg gcatgcccac gttgtggaag
     3781 gccaggttgt tccagccggt gggccgcgtg tcgtcgcggt agtagctgcg caggtaggtg
     3841 tagaggtagt cggcgccgct gccgccgcgg ccgccgcgcg agcgggcgat cacggtcagg
     3901 tcgggcggcg tcgcgccgaa ccattccttg gcctggcgcg ggtcgagcgc gatcttcatc
     3961 gtctcgccga ccttgtcggt ggcgaacagc aggttgttct tgatctgcac atcggtcagg
     4021 ccgatgtcct tcaggcggtt gtagcgcatg aaggccgccg catggcagtt caggcagtag
     4081 ttgacgaaca tcttggcgcc gcgctgcagc gccgccacgt cggtcagctt gtcggccggg
     4141 aacttgtcca gctccatgcc gccctcgctg gcacgggcag ccgacatcag cgcaaggcta
     4201 gccagcagga ttgcgatcag ctttttcatg ttggagtctc tcgcaggccg gtcaatgggc
     4261 ggaataggtc acgcgctcgg gcaccggctt gaagctgccc agccgactcc accagggcat
     4321 cagcaggaag aagccgaagt acagcagcgt accgatcatc gagacatacc cgccgacagg
     4381 cgaaggcggc tggatgccga gatagcccag cacgatgaag acggcgacga agacggcgta
     4441 gacgtatttg tgccagtccg gacggtagcg gatcgacttc accgggctgc ggtccagcca
     4501 gggcaggaag aacaggatga tcaccgctcc gcccatcacc accacgcccc agaacttggc
     4561 ctcgaagctc cacagcgccg cgacgccgac gagcccgccg atcgccacgg ccagcttgcc
     4621 cgtggacgac aggccgccgc gcagcagggc cagcacgacg gcgaccacga gcagcgcgat
     4681 cagcacatag accatctggt cggtggtggc gcgcagcatc gaatagaagg gcgtgaagta
     4741 ccacaccggc gcgatatgcg gcggcgtctt cagcgggtcg gccgggatga agttgttgta
     4801 ctcgagcaaa tagccgccgc cctcgggcac gaagaacagg atcaggctga agatgaagag
     4861 gaacaccgac acgcccatca ggtcgtgcac cgagtagtag ggatggaagg ggatgccgtc
     4921 cagcgggatg ccacgggcgt ccttcttttc cttgatctcg atgccgtccg ggttgttgga
     4981 gcccacctcg tgcagcgcga tgacatgcgc caccaccagg cccagcagca ccagcggcac
     5041 ggcgatgacg tggaagctga agaagcggtt cagcgtcgca tcgctcacca catagtcgcc
     5101 gcggatcatc agcgagagct cgttgccgat cagcgggatg gcggagaaca ggttcacgat
     5161 cacctgcgcg ccccagtagg acatctggcc ccagggcagc agatagccca tgaaggcctc
     5221 ggccatcagg cacaggaaga tcgcgcagcc gaagatccac accagctcgc gcggcttgcg
     5281 gtagctgccg tacatcagcc cgcggaacat gtgcagatag acgacgatga agaaggccga
     5341 ggcgccggtg gagtgcatgt agcggatcag ccagccccag ggcacgtcgc gcatgatgta
     5401 ctcgaccgat gcgaaggcga ggctcgcgtc gggcttgtag ttcatcacca ggaagatgcc
     5461 ggtgacgatc tggatcacca gcaccagcag cgccagcgag ccgaagaagt accagaagtt
     5521 gaagttcttc ggcgcgtagt actcggacag atgctccttc cagagcttgc tggccggaaa
     5581 gcggttgtcg acccagttca gcagcttcat agccgccggc gcgtcggggg agatttcttt
     5641 gaattcagcc atgttgtcct ctgcggtccg ggcgttcagg ccttcttcgc ttcgccgatc
     5701 aacagcttgt tgtccgacag gaaggtgtag ggcgggacct cgaggttgtc cggcgcgggc
     5761 ttgttcttga acacgcggcc ggccaggtcg aaggtcgagc catggcaggg gcagaggaag
     5821 ccgccggccc agtcatcggg cagcgaaggc tgggcgccgg gcgcgaactt ggcgctgggc
     5881 gagcagccca gatgcgtgca gatgcccacg cagacgaaga tctcgggctt gatggagcgc
     5941 cactcattgc gcgtgtcctt catcggcgtg atgtcgtcgg gcttgcgctc ggactggggg
     6001 tcggcgacca ggccgtcggt cttcttgagc gaggccagca tctcgggcgt gcgcttgacg
     6061 atccacaccg gcttgccgcg ccattcgacc gtcatcatct cgccgggctt cagtgcgctg
     6121 atatcggctt cgaccggcgc accggccgcg cgcgcgcgct cggagggttc gaaggtgctg
     6181 acgaagggga ctgcggtggc cacaccgccc acgccgccgg cggcgcaggc gatcgccacc
     6241 caggttcggc ggcctttgtc gacttggctt tcacctttgt tatcgctcat gaaggtcctc
     6301 ggctcgactt ctgattgggg caaccgggaa ttctagcgga cagccgcggc accatagaac
     6361 cggctgtcgg ctttgcgccc aggcccgcat cgcgcggcat gcgcgcgggg agtggcgcag
     6421 caccgatccc ggcttgactt gcaaggcggc tgaggaatac tgccggcggc gctcgccagg
     6481 cggcgcgcag gcttcctcga aaacaccaca ggagtcttac agcatgggat tcatgaccga
     6541 gttcaaggag ttcgccctca agggcaacgt gatggacctc gccgtcggcg tgatcatcgg
     6601 cggcgccttc ggcaagatcg tcgactccgt cgtcaacgac ctcatcatgc cgctggtagg
     6661 gagggtgacc ggtggcctgg acttctccag ctactacatc gcactggcgg gccaggcgcc
     6721 gggcctgccc ctggccgatg ccaagaaggc cggcgccgtg ttcgcctacg gcagcttcct
     6781 cacggtcctg gtctacttcg tcatcctggc cttcatcatc ttcatgatgg tcaagcagat
     6841 gaaccgcctg aagaaggagg cgccagcccc ggcgccggcc gcgacgcccg aggacgtgct
     6901 gctgctgcgt gaaatccgcg attcgctgaa gaagtagtcg actgcaaacc ggaccggcgg
     6961 tcccccgccg gcgcccgccc ggtcggtgcg ccccttgcat gcgggtcaca cggcatggga
     7021 cggtccgatt tcgggccata cttcgatgta acccggaacg gcgcagcaga ccggcgccgc
     7081 tgagccttcc ttcgacggcg tgcccctgat cagcccgtga ccgactcccg actccagccc
     7141 gccttgcaga cggccatcgc gcgcatccag gcgacggcac aggccgtggc cgatcaggtt
     7201 gcggatgcgc tgggcctggc cgccgtgtcg gccgccagca atactcagcg caaggcctac
     7261 atgacggcgc agttcgatct gcgccgcaag caggcggctt ttaacctcac cttcggcaac
     7321 gtgctgcgcg acaaggtgat gcaggaggcg ttgcctgggg tgcgcggcca acacagcctg
     7381 gcggcgaccg actgggcctc gctgagcctg gtcgacgatg ccgaggtcga ggcacaggtc
     7441 tcggccgagc ggctggcgct ggaagtcgag cacgaatgcg aatgggagct gcgcgaactg
     7501 gcggcctaca ccggatcgct gctgggcctg ggccatgccg accaggcacg caatccgctg
     7561 cgccccgaaa tgatcggcaa ggccctgttc cgcggcatcg agacggtcag cgatcaaccg
     7621 gagctgcgca agctgctggc gcgcgagctc agccgcagcc tggcccaggc aatgcgcgcc
     7681 tgctaccagt ccatcgtcga ggatttgcga cgccgcggcg tgcagccggt gggcctgaac
     7741 gtcaagcaag tcgaagggcc gggcaacgaa atggcccgcg aagttccgcg cgaaacccaa
     7801 agcggatacc agtcggccgt caagcgcagt gaagccgagg ccgccaaggc cctgagcgcg
     7861 atgttcggca tggccatgcc gcagggcctg catgatctcg gcacgcatgc cggctcgggc
     7921 cccggcagcc tgagcggcat gaccatgccg gatccggcga gcctcctgcg gacgggcgcc
     7981 tcgacgcgcg gcggcgcgtc gggcttgggc tcgggctacg gcgatctcgg cagccagagg
     8041 ggggcgccgg cgcggggctc ggatgcgcag ctgaccgagg tgatccgccg cctggccttt
     8101 ctcaccagcg gctacggcga tctgggcggc gaagctccca cgaaacccgc ctcgctgagc
     8161 ggcggggcgc tgcgtccggg cgcaccggag cgccgcttca ccgtcaccgg cggcggcggc
     8221 tacggcagct ccggccatgg cggcctgagc agcggccacc acctgcaggg cggcgtgacc
     8281 ggctacgacg ccctcaccgg cctgatggcg gctaacctga tccaccagca ccgggaggag
     8341 ctgaggcggg cttccaccgg cgcactcgat cacatggtga tcgatgtggt cggcgccctg
     8401 ttcgaccagg tgctgtccga ccccaaggtg gcgccggcca tggcgcgcca gatcgcgcgg
     8461 ctgcagctgc cggtgttgcg cgccgcgctc aaggacgtcg gcttcttcag ctcgcggcgc
     8521 catccggtgc gccgcttcgt caaccgcatc gcctcgctgg ccgcggccta tgacgacttc
     8581 gacgaaggcc tgggcaagga cttcctcgac aaggtgcgct cgctggtgca gcagatcgtc
     8641 gagggcgact tcgaccagat ggagctctac gactccaagc tggccgagct cgagtcgctg
     8701 atccagcagc agaccgccag ggaggcgccc cagcatgccc aggtggcggc gctgctggac
     8761 gacaaggaaa ccgagctgcg catccagcaa cgctacatgc gcgagctgca ggcgcatctg
     8821 gccccgctgg ccctgcacga attcctgcgc gagttcctgt cccaggtgtg gagccaggtg
     8881 caggtgctgg ccgtcaagcg tgaggggccg gattcagcgc tggccaagcg tgtgaacgcg
     8941 ctgccgcgcg agctggtctt cagcgtgcag cccaagggca cgccccagct gcgcaaggaa
     9001 ttcctgctca agctgccgca gctgatgaag gacctcaacg agggcctggc cctgatccgc
     9061 tggcccgagg cggccaagaa ggatttcctc gccaagctgc tgccggccca tgccgaatcg
     9121 ctcaagggcc agccgctcac cgacttcgag cagcgccagc tctcgcacca gctggcgcag
     9181 gtcgagaaag tcgcgatccc caaggtgcag gacgtggcgc gggattcatc catcctgccg
     9241 ctggcggcgg acgacgcgat cgagcccagc ttctcgcccg aggagatgaa gcaggtcggc
     9301 ctggtcgagg aaagcgcgat cgactgggac ggcaagatcg acatcgacct ggaccaggcc
     9361 gccgactcga cgccggccga gctggatgtc aacctcgaca gccccgcgcc gccctcttcg
     9421 ggcatccagc tgatgcatca cgtgcagccc ggcatcgcct atcgcatgca cctcgacaag
     9481 ggctggcaga aggtgaggct gaactgggcc agcccgggcc gcgccttctt cgtcttcagc
     9541 tatggccgca agcacccgaa gaccctgtcg ctgagcgccc gcatgctggc ccgcatgtgc
     9601 gaaacgggcc gcttcaaggc cttcgagcag accgagctga tcgagcgcgc gaccgcccga
     9661 gcgcgcaagc agcttgccga gctgacggcc gcggcgagca agcattgagc atcgatcaat
     9721 tcaggacatt tctgtcctga acgctcctgc gtccgacccg cttattcgtt cgggtcagcg
     9781 ttatccgatt cgcaatagct ttcgtttcag gacatttcca ctcgtctgcc gcccgcaagg
     9841 cgccgcgcca tctgcagcgc agcgatcagg ctcgccggat cggcccggcc cgtgccggcc
     9901 agatcgaagg ccgtgccgtg gtcggggctg gtgcgcacga agggcagccc cagcgtgacg
     9961 ttcacgccct gctcgacgcc caggtacttc accgggatca gcccctggtc gtggtattgc
    10021 gcaacgacca gatcgaattc gccgggatgc cctggcgcgc tgcgcgcccg catgaaaacc
    10081 gtatccggcg cgtaggggcc gctcgcagct atgccctcgg cgcgcgcgtc ggcaatcgcc
    10141 ggcgcgatcg catgcaattc ctcgtcgccg aacaagccgc cttcgccggc atgcgggttc
    10201 agccccgcca ccgcgatgcg aggacgcggc tggccccaga gcgccgcgct gcgatgcgcg
    10261 atgcgcaggg tctccagcac ggcctcgcgc gtgacgcact cgatggcccg gcgcagcgag
    10321 acgtggatgc tcaccagcac ggtcttgagc tcctcgttgg ccagcatcat gcgcacggcc
    10381 tgcgcgccgc tcagcgcctg cagcatctcg gtgtggcctg ggtaggaaac acctgccgcc
    10441 gcgaaggcct ccttgtggat gggcgcagtc acgaccgccc ccacctcgcc gcgcatggcc
    10501 aacgctacgg ccctgcgtat gcaggccgct gcggccgccc cggcgcgggc atccacccgg
    10561 cccagcgggg cctgcagcaa gtcgccgggc aatccttcga cctgcagcac gggcaggcaa
    10621 tccggcgggc aggccggaac atcggcaggg acctcgatct cgaccaccgg caacaagcca
    10681 ccagtgagct gcgcggcgcg gcgcatcacg ccgacatcgc cgagcacgaa gccgcttgaa
    10741 accgcaccgc gccgaaaggc cattgcgagg atctcggggc cgatgccgca ggcgtcgccc
    10801 atggtcagcg ccagtggaaa aagttccgtc gatttcgtct gcatggccac gattgtcaca
    10861 gcgcttacaa ggccacgcgc actgaaaagc aaagtctgtt gcttcgcaac atgacttcac
    10921 acatcgcttc cttgctcttc acgcttgcgc cctcaagatc acccctgtcg cggccctcat
    10981 cgcatcgccg cggcagcgtg ttcaacaaaa ggagttcgtt ttgaaccaaa agctcgccaa
    11041 aaagaccttc gtcgctctcg ccttcgccgc cgcggccctg cccggcttcg cgcaaaacgc
    11101 ctacgtcggc ggcaacatcg gctcgccgga ctggaagacc ggcagcatca gcggcatctc
    11161 cggggacagc aagggcacgg cctacaagct gtacggcggc tattccttca accccaactt
    11221 cgccctcgaa ggcggctggg ccgatctcgg caagctcagc ggcaatgcgg gtgctaccac
    11281 cggccgcggc ctcttcgtcg acgccgtggg ccgcttgccc ctggccaacg acttctccgc
    11341 catcggccgc gtgggcgtgt tcaacggcaa gttgagcaac ccggtcaaca gcggcaccgg
    11401 caccgacttc aagtacggcc tgggcgtgca gtacgacctg agcaagaaca tggcgctgcg
    11461 cggcgaatgg gagcgctatc gcttcaatgc cctcaacatc aagcccaatg tcgactcgct
    11521 gtcggtgggc ctgacctacg gcttctaagc cgcccggcct gaggagctga tcgaggccgg
    11581 cccgaggggg tcggcctgcg agcggcgagt gcttggcagc gcgagtctgc gctgcgacac
    11641 tcgccgcttc gcattcaact ccaagcccat gctc
//
LOCUS       DASUCY010000370         2163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_666706, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000370 DASUCY010000000
VERSION     DASUCY010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666706"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VFV25_07420"
     CDS             <1..232
                     /locus_tag="VFV25_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HEY1393189.1"
                     /translation="ITANYDGANKHRTVIGADVHVGSNCVLVAPVTLGDGATIGGGST
                     IAKDAPAGQLTVARAKQVTLPGWQRPRKKQQP"
     gene            241..474
                     /locus_tag="VFV25_07425"
     CDS             241..474
                     /locus_tag="VFV25_07425"
                     /inference="COORDINATES: protein motif:HMM:NF025733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich CWC family protein"
                     /protein_id="HEY1393190.1"
                     /translation="MTPSSPQAGPGVDPARCPLCGADNRCVMAAQRGGSDAAKPCWCS
                     EVRIAPETLARVPAEACGKACLCSRCALGLAEE"
     gene            complement(498..1184)
                     /locus_tag="VFV25_07430"
     CDS             complement(498..1184)
                     /locus_tag="VFV25_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEY1393191.1"
                     /translation="MQLYIGNKNYSSWSMRPWVLMTQAGIAFEERVLRFDSFGPDSSF
                     KREVARLSPAARVPLLVDRGFTVWDSLAIAEYLAERFPERRLWPEEAQARARARSICA
                     EMHSGFSALRNHFPMNIEASLPEVGQRVLAEQAAVRADLARIEQMWGGLLDQHRGPFL
                     FGRFSIPDAYFAPVCTRLHTYAAPMSAPIEAYVERVMQTPAVQAWVRDALAEQDFVAF
                     DEPYRKSREG"
     gene            complement(1309..>2163)
                     /locus_tag="VFV25_07435"
     CDS             complement(1309..>2163)
                     /locus_tag="VFV25_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HEY1393192.1"
                     /translation="PARAKALAMLPGVELQAADVHDEAQLSAVLRGCEAAVNLVGILH
                     GSEREFQHAHVELPRKLARACASAGVQRLVHVSALGVAADAPSRYLRSKAAGEAVLRE
                     EARLDLTILRPSVMFGAEDRFLNLFASLQALFPLLPLAGSEAQFQPVWVEDVAAAITA
                     CLTDERPSAGLAIECAGPKVYTLRELVRLAGRWSGHERPVLPLPMPLGRLQAGLMSLL
                     PGEPLMSPDNLDSMRVPNVASGKLPGLSSLGIQATALEAVAPDYLGAQAGPGCGRFDG
                     WRALARRR"
BASE COUNT          357 a          758 c          749 g          299 t
ORIGIN      
        1 catcacggcc aactacgacg gcgccaacaa gcaccgcacc gtcatcggcg ccgacgtgca
       61 cgtgggctcc aactgcgtgc tggtggcgcc cgtgacattg ggcgatggcg cgaccatagg
      121 cggcggcagc accatcgcca aagatgcacc ggccggccag ctcactgtgg cgcgcgcaaa
      181 acaggtgacg ctgccgggct ggcagcggcc acgcaagaag cagcagcctt gagcacagca
      241 gtgacgccct cctccccgca ggccgggccc ggcgtcgacc cggcgcgctg cccgctttgt
      301 ggggcagaca accgctgcgt aatggcggcg cagcgtggcg gcagcgatgc ggccaagcct
      361 tgctggtgca gcgaagtacg catcgcgcct gagacgctgg cgcgcgttcc tgccgaggcg
      421 tgcgggaagg cctgcttgtg ctcgcgctgt gcgcttgggc tcgctgagga gtgaggccgt
      481 agtcagaggg ttcgccccta cccctcccgc gacttcctgt aaggctcatc aaacgccaca
      541 aagtcctgct cagccaaagc atccctgacc caggcctgca cggccggcgt ctgcatcacg
      601 cgctcgacat aggcctcgat cggcgccgac attggcgcgg cataagtgtg caagcgggtg
      661 cagaccggcg cgaaatacgc gtcgggaatg ctgaagcggc cgaacaggaa gggcccgcgg
      721 tgctggtcca acagcccgcc ccacatctgc tcgatgcgcg ccagatcggc gcgcaccgcc
      781 gcctgctcgg cgagcacgcg ctggcccacc tcgggcaggg aggcctcgat gttcatcggg
      841 aaatggttgc gcagcgccga gaagcccgag tgcatctcgg cgcagatgct gcgcgcgcgg
      901 gcccgcgcct gggcttcttc gggccatagg cgccgctcgg ggaagcgctc ggccaggtat
      961 tcggcgatcg ctaatgaatc ccagaccgtg aagccgcggt ccaccagcag cggcacacgg
     1021 gcggccgggc tcagcctggc gacctcgcgc ttgaagctcg agtcgggccc gaagctgtcg
     1081 aagcgcagca cgcgctcctc gaaggcaatg ccggcctggg tcatcagcac ccagggacgc
     1141 atggaccagg acgagtagtt cttgttgccg atgtagagct gcatggccag gcctccgcgc
     1201 tgattgacga ttgaatgcag cgcgatgcta gtcgaggcga tgccgtcaag cggtgatttc
     1261 ggcgcaagcg attgatgcgc cgcgcgcatg cccgtcgtgt gagcagccct agcgtcgccg
     1321 cgccagcgcc cgccagccgt cgaaacgccc gcagcccgga cctgcctggg cgccgaggta
     1381 gtcgggcgcc accgcttcca gcgccgtggc ctgtatgccc aggctgctca agccgggcag
     1441 cttgccgctg gccacattgg gcacgcgcat cgaatcgagg ttgtcgggcg acatcagcgg
     1501 ctcgccgggc aggaggctca tcaggccggc ctgcaggcgg cccagcggca tcggcagcgg
     1561 cagcacgggc cgctcgtggc cggaccagcg ccccgccagg cgcaccagct cccgcagcgt
     1621 gtagaccttg ggaccggcgc attcgatggc gaggccagcg ctcgggcgct cgtcggtcag
     1681 gcaggccgtg atcgcggccg ccacgtcttc cacccacacc ggttggaact gcgcttcgct
     1741 gcccgccagc ggcagcagcg ggaacaaggc ctgcagcgag gcgaagagat tgagaaagcg
     1801 gtcctcggcg ccgaacatca cgctgggccg caggatggtg agatccagcc tcgcctcctc
     1861 gcgcagtacg gcctcgcctg cggccttgct gcgcagatag cgcgaaggcg catcggccgc
     1921 cacgccgagc gcgctgacgt gcacgaggcg ctgcacgccg gccgatgcgc aggcgcgggc
     1981 cagcttgcgc ggcagctcga catgcgcatg ctggaactca cgctcgctgc catgcaggat
     2041 gcccaccaga ttgacggccg cttcacagcc ccgcagcacc gcggagagct gggcctcatc
     2101 gtgcacatcg gcggcctgca gttctacgcc cggcagcatc gccagggcct tggcgcgcgc
     2161 ggg
//
LOCUS       DASUCY010000371         6710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1116472, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000371 DASUCY010000000
VERSION     DASUCY010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 6710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6710
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1116472"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="VFV25_07440"
     CDS             complement(<1..738)
                     /locus_tag="VFV25_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase kinase/phosphatase AceK
                     regulatory subunit"
                     /protein_id="HEY1393193.1"
                     /translation="MFPHQLTDARAFNIARAMLEGFNRHYRLFREASAAAKTRFEAAD
                     WHGQQRAQAQRIEFYDKRVDEAVERLKREFNAHQLPMDVWQQVKLHYIGLLTNHHQPE
                     LAETFFNSVCTRILHRSYFNNDFIFVRPAISTEYIENEESPTRPTFRAYYPGSREGMA
                     ACFERIVHNFQLELPFEDLRRDVGYVVRAVAEHFGDLRIAPNFQIHTLSSLFFRNKTA
                     FIIGRVLNGDQTYPMAIPVMHGPSGKLV"
     gene            complement(744..1403)
                     /gene="can"
                     /locus_tag="VFV25_07445"
     CDS             complement(744..1403)
                     /gene="can"
                     /locus_tag="VFV25_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HEY1393194.1"
                     /translation="MSTRLDELFESNRRWAAKTEAREPGFFSRLLKQQAPEYLWIGCS
                     DSRVPANELVDLLPGELFVHRNVANVVVHSDLNCLSVIQFAVDMLKVKHIIVVGHSNC
                     GGVVAALKGSRIGLADNWLRHVHDVRNKHEAWLDSLPAERRLAALVELNVLEQARNVC
                     ETTIAQDVWQRGQELVVHGWVYGLDNGLLKDMSITVARPEDLAEAYAGALAALRRRHE
                     A"
     gene            complement(1468..1911)
                     /locus_tag="VFV25_07450"
     CDS             complement(1468..1911)
                     /locus_tag="VFV25_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY1393195.1"
                     /translation="MATNAVAHAANDKNASYSIGELAREFDLTTRAIRFYEDCGLLSP
                     ERRGPNGRIRVYSARDRTRLKLTLRGKRLGLSLAEVKELVDMYESPRDTQAQLKKFLV
                     VLAEHRAQLEQQLADLQVTLDEVCAHEKDSRRLLAQIETPRKKLA"
     gene            1962..3017
                     /locus_tag="VFV25_07455"
     CDS             1962..3017
                     /locus_tag="VFV25_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY1393196.1"
                     /translation="MNELEKQLGYPLGETLPAVGATLEVAPGVRWVRMLLPFALDHIN
                     LWLLRDEIDGRQGWSIVDCGIANDATRAAWEQVFATQLDGLPVLRVIVTHMHPDHMGL
                     AHWLTEKWQCRLWVSATDYNVARMATQSTVGFGGDAAASHFSRNGLTDPESLAKVRAR
                     ANYYASMVPAVPASFRRLMQGQTLTIGGHAWRCIAGYGHAPEHISLHCEALGVLISGD
                     MVLPRISTNVSVIDVEPEADPLRLFLASLAELCALPAMTLVLPSHGRPFTGLHTRIEQ
                     LQAHHDKHFAEVMQACAEHPRSGADILPILFKRKLDLHQTTFAVGEAIAHLHALWYAG
                     KLERRLDDDGVYRFSAR"
     gene            3098..3688
                     /locus_tag="VFV25_07460"
     CDS             3098..3688
                     /locus_tag="VFV25_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010399329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEY1393197.1"
                     /translation="MARALKSLALLAAACYLIAAGVIAAAGLQDHLEPADAIVVFGNA
                     VAADGTPSRRLGARLDGALEVYRRGLASLVIVSGGIDREGHDEAATMARYLEDRGIPR
                     SAIVQDNAGKDTMATAADVARIASERRLESVIVATQYFHVPRARLALMRAGLRVVGSV
                     HARYFEARDFYSLAREVVGFAAYYVGLRSPAERTSS"
     gene            complement(3693..5018)
                     /locus_tag="VFV25_07465"
     CDS             complement(3693..5018)
                     /locus_tag="VFV25_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HEY1393198.1"
                     /translation="MPELTRLDDLHLWYLGDPATPRYIGALKLVSAGKGVSLRYGREW
                     LDHGFALSEDLPLVDIEHLPPGRLAADAQRAVGAVDDARPDRWGEKVIRFVDKPKRLS
                     LMEYLYYAGDDRFGALGVSTSSSVYSPRAGSPLPRLEAAQQLGEVVTKIEASEPISAL
                     EAKLVRAGGSLGGAKPKALIDIGGEQWVIKFFNGEPIDTPLVEHATMTLAERAGITVA
                     KTQVVPLAGANAVAVRRFDREQGRRIHSISAGTAIRAVTAPGDEPEMGYPELARILRR
                     VGITQDDINQRDARELFRRMVFNILIDNTDDHEKNHSLLVVSPFENGRLKLAPAYDVL
                     PTNSGQGYQEFICGAQGRDSTLENAMSQCDAFGLLPAQAAAEAAAVIDVVNTWQEHFA
                     QAGVTQRDIESLAQRIDGEQLLAQRTDFDQARFGSAAAKRKRTSPFRRT"
     gene            complement(5020..5247)
                     /locus_tag="VFV25_07470"
     CDS             complement(5020..5247)
                     /locus_tag="VFV25_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393199.1"
                     /translation="MPTLQRLEAGDPSVGIGIVATALWLIKRDAELRNLAAPEHDQGA
                     IEMDVREALELGRARAQASAEARTRKNQTKG"
     gene            complement(5563..6606)
                     /locus_tag="VFV25_07475"
     CDS             complement(5563..6606)
                     /locus_tag="VFV25_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY1393200.1"
                     /translation="MAHSALAAAAAALACGAHAQSADPYNPSAYLLPNISAIKPDGNW
                     PAHNTGFGGGIKFGQPLSPSWDLQAGVNYARSKENGLSYSQTLLGVDALYLFNPSGWR
                     PFLLGGLGAERDSADLVGGGSRSATRPYVNLGAGLQYRWSPTLGFQADVREVYGFLNN
                     NDFGFKRSSNTYFNLGLIWAFGGAPAAAAPMAAAVAAPPPPPPPAAKAPPPPPPPPPP
                     PKMQKFTLQASELFGFGSAKLSGNQPKLDEVASTLNANPGISKVVIAGYTDRLGTKAG
                     NQKLSQQRAESVRSYLIGKGVAGGRLEAVGKGDADPVAGCKDVKKHAELVKCLEPNRR
                     VEIEPVSYERAVK"
BASE COUNT         1070 a         2214 c         2258 g         1168 t
ORIGIN      
        1 cacgagcttg cccgacggcc cgtgcatcac cgggatcgcc atcgggtagg tctggtcgcc
       61 gttcaggacc cggccgatga tgaacgccgt cttgttgcgg aagaacagcg acgacagcgt
      121 atggatctgg aaattcggcg cgatgcgcag gtcgccgaaa tgctccgcca cggcgcgcac
      181 cacgtagccg acgtcgcggc gcaggtcttc gaacggcagc tcgagctgga aattatggac
      241 gatgcgctcg aaacaggccg ccatgccttc gcggctgcca ggatagtagg cacggaacgt
      301 cgggcgcgtg ggcgattcct cgttctcgat gtactcggtc gagatcgccg gacgcacgaa
      361 gatgaaatcg ttgttgaaat acgagcggtg caggatgcgc gtgcagaccg agttgaagaa
      421 ggtctcggcc agctcgggct ggtgatggtt ggtcagcagg ccgatatagt gcagcttgac
      481 ctgctgccac acgtccatcg gcagctggtg cgcattgaac tcgcgcttga gccgctccac
      541 cgcctcgtcg acccgcttgt cgtagaactc gatgcgctgg gcctgggcgc gctgctggcc
      601 atgccagtcg gccgcctcga agcgggtctt ggctgccgca ctggcctcgc ggaacagcct
      661 gtagtggcgg ttgaagcctt ccagcatcgc gcgtgcgatg ttgaaggctc tcgcgtcggt
      721 gagctggtgc ggaaacattg cgctcaggct tcgtggcggc gcctgagcgc ggccaatgcg
      781 cccgcatagg cctcggccag atcctcggga cgggccaccg tgatgctcat gtccttgagc
      841 aggccgttgt cgagcccgta gacccagccg tgcacgacca gctcctggcc gcgctgccag
      901 acgtcctgcg cgatcgtggt ctcgcacaca ttgcgcgcct gctccagcac attgagctcg
      961 accagcgcgg ccaggcggcg ctcagccgga aggctgtcta gccaggcctc gtgcttgttg
     1021 cgcacgtcgt gcacgtggcg cagccagttg tcggccaggc cgatgcgcga gcccttgagc
     1081 gccgccacca cgccgccgca gttcgaatgg ccgaccacga tgatgtgctt gaccttgagc
     1141 atgtcgaccg cgaactggat caccgagagg cagttgaggt cggagtgcac caccacgttc
     1201 gccacgttgc gatgcacgaa gagctcgccc ggcagcaggt cgaccaactc gttggccggc
     1261 acgcggctgt cgctgcagcc tatccacagg tattcgggcg cctgctgctt gagcagccgc
     1321 gagaagaagc ccggctcgcg cgcctcggtc ttggcggccc agcggcggtt gctttcgaag
     1381 agttcgtcga gtcgggtaga catgttactg attaagtact gaatatcgtc aaatcactaa
     1441 gggaaatgcg caagaaagtt gtgaaaccta ggccagcttc ttgcgcggcg tctcgatctg
     1501 cgccagcagg cgccgcgagt ccttctcatg ggcacagacc tcgtccagcg tgacctgcag
     1561 atcggccagc tgctgctcca gctgcgcgcg gtgctcggcc agcacgacga ggaatttctt
     1621 caactgggcc tgggtatcgc gcggcgactc gtacatgtcg accagttcct tgacctcggc
     1681 caggctcagg cccaggcgct tgccgcgcag cgtgagcttg agccgcgtgc ggtcgcgcgc
     1741 gctgtagacg cggatgcgcc cgttgggccc gcgccgctcg gggctgagca ggccgcagtc
     1801 ctcgtagaag cgaatcgcgc gcgtcgtgag gtcgaactcg cgcgccagct cgccgatgga
     1861 gtagctggcg ttcttgtcgt ttgccgcgtg agcgacagcg ttggtggcca tggttgccta
     1921 gaatgggtcg gaattgacgt ttacgtaaag tgtagggcag catgaacgaa ttggaaaaac
     1981 agctcggcta ccccctcggc gaaacgctgc ccgccgtcgg cgccacgctg gaggtcgcgc
     2041 cgggtgtgcg ctgggtgcgc atgctgctgc ccttcgcgct ggaccacatc aacctctggc
     2101 tgctgcgcga cgagatcgac ggccgccaag gctggagcat cgtcgactgc ggcatcgcca
     2161 acgatgcgac gcgcgccgcc tgggagcagg tcttcgcgac ccagctcgac ggcctgccgg
     2221 tgctgcgcgt gatcgtcacc cacatgcacc cagaccacat gggcctcgcg cattggctga
     2281 ccgagaaatg gcagtgccgc ctgtgggtca gcgccaccga ctacaacgtg gcgcgcatgg
     2341 ccacgcagag cacggtgggc ttcggcggcg atgcggcggc atcgcatttc tcgcgcaacg
     2401 gcctcaccga tcccgagtcg ctcgccaagg tacgggcccg cgccaactac tacgcctcga
     2461 tggtgccggc cgtgcccgcg agcttccggc gcctgatgca gggccagacg ctgaccatag
     2521 gcgggcacgc ctggcgctgc atcgccggct acgggcacgc gcccgaacac atcagcctgc
     2581 attgcgaggc gctgggcgtg ctgatctcgg gcgacatggt gctgccgcgc atctcgacca
     2641 atgtgagcgt gatcgacgtc gagcccgagg ccgacccgct gcggctcttc ctggcctcgc
     2701 tcgccgagct gtgcgccctg cccgccatga ccctggtgct gccatcgcat ggccggccct
     2761 tcaccggcct gcacacgcgc atcgagcagc tgcaagccca ccacgacaag catttcgccg
     2821 aggtgatgca ggcctgcgcc gagcacccgc gcagtggcgc cgacatcctg ccgatcttgt
     2881 tcaagcgcaa gctcgacctg caccagacca ccttcgccgt cggcgaggcg atcgcccacc
     2941 tgcatgccct gtggtacgcg ggcaagctgg agcgccgcct ggacgacgac ggcgtgtatc
     3001 gcttctccgc ccgctagccc gtccagagac gccgcccgcc atgccctcga acagacggac
     3061 ttcggcagcc tcagaccgca ccgggcggat ggcctccatg gcgcgcgcgc tcaagtcgct
     3121 ggccttgctg gctgcggcct gctatctcat cgccgcgggc gtcatcgccg ccgccggcct
     3181 gcaggatcac ctggagcctg ccgatgcgat cgtcgtcttc ggcaacgccg tggcggccga
     3241 tggcacgccc agtcgccggc tgggcgcgcg cctggacggc gcgctggagg tctacagacg
     3301 cggcctggcc tcgctggtga tcgtgagcgg cggcatcgac agggaaggcc acgacgaagc
     3361 ggccaccatg gcccgctatc tcgaggacag aggcatcccg cgctccgcca tcgtgcagga
     3421 caacgccggc aaggacacga tggcgacggc ggcggacgtc gcccgcatcg ccagtgagcg
     3481 caggctggaa tcggtcatcg tggcgaccca atacttccac gtgccgcgtg cccgcctggc
     3541 gctgatgcgc gcggggctgc gggtcgtggg ctcggtgcac gcccgctact tcgaggcccg
     3601 cgatttctac tccctcgccc gcgaggtcgt ggggtttgcg gcctattacg tcgggctgcg
     3661 cagccctgcc gagcgcacaa gctcctgatt tgtcaggtgc gccggaaagg gcttgttcgc
     3721 tttctcttgg ccgccgctga accaaacctg gcctggtcga agtcggtgcg ctgagccagg
     3781 agttgttcgc catcgatgcg ctgcgccagg ctctcgatgt cgcgctgcgt cacgccggcc
     3841 tgcgcgaagt gctcctgcca ggtgttcacg acgtcgatga cggcggcagc ttcggccgcg
     3901 gcctgcgcgg gcagcaggcc gaatgcatcg cactgcgaca tcgcgttttc gagcgtcgag
     3961 tctcggccct gagcgccgca aatgaactcc tggtatccct gccccgagtt ggtcggcagg
     4021 acgtcgtacg ccggcgccag cttcaagcgg ccattctcga atgggctcac gaccagcagg
     4081 gagtgattct tctcgtggtc atcggtgttg tcgatcagga tgttgaagac catgcgacgg
     4141 aagagctcgc gtgcgtcgcg ctggttgatg tcgtcctggg tgatgccgac gcggcggaga
     4201 atccgcgcca gttcagggta tcccatctcg ggttcgtcgc cgggcgcagt gaccgcgcga
     4261 atggcggtgc ccgctgaaat gctgtgaatc cgccggccct gttcgcggtc gaagcgacga
     4321 acagcgactg cgttcgcgcc tgcgaggggg acgacctgcg tcttggccac agtgatgccc
     4381 gcgcgctcgg caagcgtcat cgtcgcgtgc tcgacgagcg gcgtgtcgat gggctcgcca
     4441 ttgaagaact tgatgaccca ttgctcgccg ccgatgtcga tgagcgcctt gggcttcgcg
     4501 ccgccgaggc tgccgccggc gcggacaagc ttcgcctcca aggcgctgat gggctccgag
     4561 gcctcgatct tggtcactac ctcgccaagc tgctgcgccg cctcgagccg aggcagcgga
     4621 cttcccgctc gcgggctgta aacactcgat gacgtggaaa cgccgagcgc gccgaatcgg
     4681 tcgtcgccgg cgtagtacag gtactccatg agggagaggc gcttgggctt gtcaacgaag
     4741 cggatgacct tctcgcccca acggtctggt cgcgcatcgt cgactgcgcc gaccgcgcgc
     4801 tgcgcatcgg ccgccaaacg gcctgggggc aggtgctcga tgtcgacgag cggcagatcc
     4861 tcgctcagcg cgaacccgtg gtccagccac tccctgccgt agcgcaagga gacgcctttg
     4921 ccggcggaga ccagcttcag tgcgccgata tagcgcggtg tggccgggtc acccaggtac
     4981 caaaggtgca ggtcgtcaag tcgggtcagt tccggcatct cagcccttcg tctggttctt
     5041 gcgcgtgcgc gcttcggcag aggcctgggc gcgcgcgcgg cccagttcca gggcctcacg
     5101 gacatccatc tcgatggcgc cttggtcgtg ttcgggcgcc gcgagatttc tcaactctgc
     5161 atcccgcttg atgagccaca gcgcagtggc gacgatgccg atgcccacgg acggatcgcc
     5221 ggcttcgagg cgctgcagcg tgggcacgga aacccccatg cgtttcgccc aggtcttcaa
     5281 ggactccttg cggcgcaagc gggccacggc caggtcggcg ccaagtttct cgattgcggc
     5341 ggccgtcgca ggtgggagat gcaggagggc acgggcggtt ttcggcatac acatagatca
     5401 taacgaaata ggcacatccg tcaatatcta tgtgtacaag cgcaccaaga atctcttctt
     5461 gcctcttgtc gaaccactct caacacctga cgcgaacagc gccaaaaaga aagcccgccg
     5521 gctccgaccg gcaggcttcc tcgtaaggct tgtcgctgcg ggctacttga ccgctcgctc
     5581 gtagctcacc ggctcgatct cgacgcgccg gttcggctcc aggcacttga ccagctcggc
     5641 gtgcttcttc acgtccttgc agcccgcgac gggatcggca tcgcccttgc ccacggcctc
     5701 caggcggccg ccggccacgc ccttgccgat cagatagctc ctgaccgact cggcacgctg
     5761 ctgcgacagc ttctggttgc cggccttggt gcccaggcga tcggtgtagc cggcgatcac
     5821 gaccttgctg atgcccgggt tggcgttcag cgtgcttgcc acctcgtcca gcttgggctg
     5881 gttgccggac agcttagcgc tgccgaagcc gaacagctcg ctggcctgca gcgtgaactt
     5941 ctgcatcttg ggcgggggcg gcggaggcgg tgggggcgga ggcgccttag cagctggcgg
     6001 gggcggcgga ggcggcgccg cgacggcggc agccatcggc gccgcggcgg caggcgcacc
     6061 gccgaaggcc cagatcagcc ccagattgaa ataggtgttg ctgctgcgct tgaagccgaa
     6121 gtcgttgttg ttgaggaagc catagacctc gcgcacatcg gcctggaagc ccagcgtggg
     6181 cgaccagcgg tattgcaggc cggcgccgag gttgacgtaa ggccgggtgg cggagcggct
     6241 gccgccgccg accaggtcgg cgctgtcgcg ctcggcaccc aggccgccga gcaggaaggg
     6301 ccgccagccg ctggggttga acagatacag cgcatccacg cccagcagcg tctgcgagta
     6361 gctcaggccg ttctctttgg agcgcgcata gttcacgccg gcctgcaggt cccagctcgg
     6421 ggacagcggc tggccgaact tgatgccgcc gccgaagccg gtgttgtggg ccggccagtt
     6481 gccatcgggt ttgatggcgc tgatgttggg cagcagatag gccgaggggt tgtagggatc
     6541 ggcgctctgc gcatgggcac cgcaagccaa ggctgctgcg gccgccgcga gagcgctgtg
     6601 tgccatgaac ttcttgtgga tcattgacga actccaagcg agtgagagga gaagcgcacg
     6661 gtacgcagcg ccactgcacc cgcggcccgg agttcgcgtc cgcttgtaac
//
LOCUS       DASUCY010000372         2661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_987983, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000372 DASUCY010000000
VERSION     DASUCY010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987983"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1421
                     /locus_tag="VFV25_07480"
     CDS             <1..1421
                     /locus_tag="VFV25_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY1393201.1"
                     /translation="FSATAVSAYHAYGSWLVSISWKRFFVLSGLLMIMAAVLQDLPPF
                     SWKFTETVDMVPSVSPPSPPSPPKPPSVKFGGKPMISTDKPTSGGDKGGVDITIDEKG
                     VRIARRPKEGAAASGAASAAATAATPAASQASAAANDEGLQIHLPPEIANNPDVKDAI
                     EEARKAVADAAADAENARRDAEDTRREAEDAKHEAEAIRIEAEKQASRALKRVHTWSV
                     GDMLVNMAWVFILASAVIKITYKGQLQAEVKAAEATEVAESEALKRQVVEARMAAMQA
                     QVEPHFLFNTLASIDHLIETDPARASKMQKNLIALLRASMPTMREANAQGTKDLGREL
                     TVIRPYLEILKVRMEDRLQTEIAVPEGLLSAEFPPMMLQSLVENAIKHGLEPKPEGGR
                     LTVRAEIQHGKLAVTVADTGLGFGQAATAGTGTGLANIRERLQLLYGAKAQLKIAENQ
                     PSGTAVTIVVPYTARQQEAEAA"
     gene            1418..1921
                     /locus_tag="VFV25_07485"
     CDS             1418..1921
                     /locus_tag="VFV25_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393202.1"
                     /translation="MSDHHSMDLQGEGAEAPQPAAPASHAGIAPQRRRRWPWFLLAAF
                     VALLLLAAFATWMGQVRIEPVHLIINGEERTLAFDLASASFFSKLGVAMAVFCVLTVL
                     AVVVPFAILLAALAVVLALVLSLGLPLMAVSLALAVVFSPVILIVWLVWWLVRRGRTA
                     PSSTIGT"
     gene            1918..>2661
                     /locus_tag="VFV25_07490"
     CDS             1918..>2661
                     /locus_tag="VFV25_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEY1393203.1"
                     /translation="MSSDHNPSSPSPRRAVIADDERLMREQLRARLTQVWPALEIVAE
                     AKNGAEAVAQVAEHHPDLVFLDIRMPGMTGVEAAREIARMDATPDWPGCEIVFITAYD
                     QYAVEAFEQGVVDYVLKPAEADRLALTVQRVVKRLAQRDQHGEEGTADALQSQLQRML
                     AQIAGKLAPGSGPERMKWVQASVGSSIQMIPVEDVLFFISDEKYTRVQTAQLEALIRK
                     PIKELTEELDAELFWQIHRSTLVNVRAISG"
BASE COUNT          458 a          922 c          899 g          382 t
ORIGIN      
        1 gcttctcggc cacggcggtc agcgcgtatc acgcctacgg cagctggctg gtcagcatca
       61 gctggaagcg cttcttcgtg ctctcggggt tgctgatgat catggcagcc gtcctgcagg
      121 atctgccgcc cttcagctgg aagttcacgg agacggtgga catggtgccc agcgtcagcc
      181 ccccgagccc gccgagcccc cctaaaccgc cgtcggtgaa gttcggcggc aagcccatga
      241 tctcgaccga caagccgacc tcgggcggcg acaagggcgg cgtggacatc accattgacg
      301 agaaaggcgt gcgcatcgcg cgccgtccca aggagggcgc agcggcctcg ggggccgcgt
      361 cggcggccgc aacagcggcc acgcccgccg catcccaggc ttcggccgcg gcgaacgacg
      421 aaggcctgca gatccatcta cctcccgaga ttgccaacaa ccccgacgta aaggacgcga
      481 tcgaagaggc gcgcaaggcc gtggccgatg cggcggccga tgcggagaac gcgcgacgcg
      541 atgccgaaga cacccggcgc gaagccgaag acgccaagca cgaagccgaa gccatccgta
      601 tcgaagccga gaagcaggcc tcgcgggcgc tcaagcgggt gcatacctgg agcgtgggcg
      661 acatgctggt caacatggcc tgggtcttca tcctcgcctc ggccgtcatc aagatcacct
      721 acaagggcca gctgcaggcc gaagtgaagg cggccgaggc gaccgaggtc gccgagagcg
      781 aggcgctgaa gcgccaggtg gtcgaggctc gcatggcggc gatgcaggcc caggtggaac
      841 cgcacttttt gttcaacacg ctggcttcca tcgaccacct gatcgagacc gacccggcgc
      901 gcgcctcgaa gatgcagaag aacctgatcg ccctgctgcg cgccagcatg ccgaccatgc
      961 gcgaggccaa tgcgcagggc acgaaggacc tgggccgcga gctgaccgtg atccggccct
     1021 atctcgagat cctgaaggtg cgcatggaag accggctgca gaccgagatc gccgtgcccg
     1081 agggcctctt gagcgccgag ttcccgccga tgatgctgca gtccctggtc gagaacgcca
     1141 tcaagcacgg cctggagccc aagcccgagg gcgggcggct gacggtgcgc gccgagatcc
     1201 agcacggcaa gctggccgtg accgtggccg acaccgggct gggcttcggc caggccgcca
     1261 ccgccggcac gggcacgggc ctcgcaaaca tccgcgagcg cctgcagctg ctctacggcg
     1321 ccaaggccca gctcaagatc gccgagaacc agcccagcgg cacggcggtg accatcgtcg
     1381 tgccttacac ggcgcggcag caagaagcgg aggcagcatg agcgaccacc actccatgga
     1441 tctgcaaggc gaaggcgccg aagcaccgca accggcggcg cctgcatcgc acgctggcat
     1501 cgcaccgcag cgccggcgcc gctggccctg gttcctgctg gccgcgttcg tggccctgct
     1561 gctgctcgcc gcattcgcga cctggatggg ccaggtccgc atcgagcccg tgcacctgat
     1621 catcaacggc gaggaacgca cgctggcgtt cgacctcgcc tcggcctcgt tcttcagcaa
     1681 actgggagtc gccatggctg tcttctgcgt actgacggtc ctcgccgtcg tggtgccctt
     1741 cgcgatactg ctggcggcgc tcgccgtggt gctggcgctg gtgctgagcc tggggctgcc
     1801 cctgatggcc gtctccttgg ccttggccgt cgtgttctcg ccggtgatcc tgatcgtctg
     1861 gctggtgtgg tggctggtgc gccgcggccg gacggcgcct tcctctacca tcggcacatg
     1921 agcagcgacc acaacccttc aagccccagc ccgcgccgcg ccgtcatcgc ggacgacgag
     1981 cgcctgatgc gcgagcagct gcgtgcccgc ctgacccagg tctggcccgc gctcgaaatc
     2041 gtcgccgagg ccaagaatgg cgccgaggcc gtggcccagg tggcggagca tcaccccgat
     2101 ctcgtcttcc tcgacatccg catgccgggc atgaccggcg tcgaggcggc gcgcgagatc
     2161 gcccgcatgg acgccacgcc ggactggccg ggctgcgaga tcgtcttcat caccgcctac
     2221 gaccagtacg cggtcgaggc cttcgagcag ggcgtggtcg actatgtgct caagccggcc
     2281 gaggccgatc ggctggccct cacggtgcag cgcgtcgtca agcgcctggc gcagcgcgac
     2341 cagcatgggg aggaaggcac ggccgacgcg ctgcagtcgc agctgcagcg catgctggcg
     2401 cagatcgccg gcaagctcgc cccgggctcg ggccccgagc gcatgaaatg ggtgcaggcc
     2461 agcgtcggca gcagcatcca gatgatcccg gtcgaggacg tgctcttctt catctccgat
     2521 gagaagtaca cgcgggtgca gaccgcacag ctcgaggccc tgatccgcaa gccgatcaag
     2581 gagctgaccg aggagctgga tgccgagctc ttctggcaga tccaccgctc gacgctggtc
     2641 aatgtgcgtg cgatcagcgg c
//
LOCUS       DASUCY010000373         2753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_939782, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000373 DASUCY010000000
VERSION     DASUCY010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939782"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VFV25_07495"
     CDS             <1..588
                     /locus_tag="VFV25_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEY1393204.1"
                     /translation="VVPLALPLQAIDIRELPRPPKGAQQARDTADLNPLLAPARAGED
                     LGLISEAGLPAIADPGAALVQAAHAAGIVVQALPGPSSLLLALAASGLNGQSFAFVGY
                     LPTDAAQRASRIRELEQASRRGAQAQLIIETPYRNAALLEALLAHLQPGTRLSLSCGL
                     SLVDGWTRSDTVAQWRARPATLPGDMPVVFCFQAA"
     gene            complement(596..2029)
                     /locus_tag="VFV25_07500"
     CDS             complement(596..2029)
                     /locus_tag="VFV25_07500"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY1393205.1"
                     /translation="MTDPIADQAPARFDTLALDAKFLRAVADSGYVTMTPIQAKAIPI
                     VLDGRDVMGAAQTGTGKTAAFSLPLLQRMLKHENTSASPARHPVRALVLAPTRELADQ
                     VARNVTAYAKHTQLRVACVFGGIDMKPQTQELKAGVEVLIATPGRLLDHIEARNCSLG
                     QVEYVVLDEADRMLDIGFLPDLQRILSYLPKQRQTLLFSATFSPEIKRLAQSYLQDPL
                     LVEVARPNATASTVEQRFYRVEEDDKRRAVRQLLRERGITQSLVFVNSKLGAARLARA
                     FERDGLKTSALHGDKSQDERLKALAAFKAGEVDLLVATDVAARGLDIVDLPAVFNFDV
                     PFNAEDYVHRIGRTGRAGASGLAITLVCRDDNRLIGDIEKLIKKKLDIEPLDLEDERP
                     RRPPRRDADRAERLDGEGSAPAYQAPRRYAPPAASADPLFDRPYEPAADAEPAAWERK
                     PAAAPATGLSRFIKPKRKVAALLGGNS"
     gene            complement(2057..>2753)
                     /locus_tag="VFV25_07505"
     CDS             complement(2057..>2753)
                     /locus_tag="VFV25_07505"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEY1393206.1"
                     /translation="VVAFPAGAALVTLFVVAGVANSINIIDGMNGLASMCTAIMLSGL
                     AYVALQEGDWLVASLAISVVGAVLGFFVWNYPRGLVFLGDGGAYFLGFILAELGVLLV
                     HRNPTVSPIFPLLLCAYPIFETIFSMYRRKLVRGHAMGMPDGIHLHTLIYRRLMRWAV
                     GNRDAEVLTRRNSMTSPYLWMLCSLSVVPSLFWWQSSAILTGFLALFVATYVFLYQRI
                     VRFKTPRALVVHK"
BASE COUNT          486 a          899 c          940 g          428 t
ORIGIN      
        1 gtcgtgcctc tggcactgcc gctgcaagcc atcgatatac gcgagctgcc gcgaccgccg
       61 aaaggcgctc agcaggccag ggacacagcc gacctcaatc ccctgctcgc ccctgcgcgt
      121 gccggcgaag acttggggct gatatccgag gccggcctgc cggccatcgc agaccctggc
      181 gcggccctgg tgcaggcggc ccatgcggct ggcatcgtcg tgcaagcgct gcccgggccg
      241 agctcgctgc tgctggcact ggctgccagc gggctcaacg gccagagctt tgccttcgtc
      301 ggctacctgc ccaccgatgc ggcacagcgc gcttcacgca tacgcgagct ggaacaggcc
      361 tcgcggcgcg gcgcccaggc gcaactgatc atcgaaacgc cttatcgcaa cgcggccttg
      421 ctggaggccc tgctggctca tctccaaccc ggcacgaggc tgagcctgag ttgcggcctg
      481 agcctggttg acggctggac gcgcagtgac accgtggcgc agtggcgagc ccggcccgcg
      541 acgctgccgg gcgatatgcc cgtggtgttc tgctttcagg cggcttgaac cggcttcagg
      601 aattgccgcc cagcaaggct gccaccttgc gcttgggctt gatgaagcgc gacagcccgg
      661 tcgctggcgc ggccgctggc ttcctttccc aggcggcggg ttcggcatcg gcggccggct
      721 cgtaggggcg gtcgaacagc ggatcagcag acgcagccgg cggcgcatag cggcgcggcg
      781 cttgataggc aggagcgctg ccttcgccgt ccagacgctc agctcgatca gcatcgcggc
      841 gcggcgggcg gcgggggcgc tcgtcctcga ggtccagcgg ctcgatgtcc agcttcttct
      901 tgatcagctt ctcgatgtcg ccgatcaaac ggttgtcgtc gcggcacacc agcgtgatcg
      961 ccagacccga ggcgcccgca cggccggtgc ggccgatgcg gtggacgtag tcctcggcat
     1021 tgaaaggcac gtcgaaattg aagacagccg gcaggtcaac aatgtccaga ccgcgcgcgg
     1081 ccacgtcggt cgccaccagc agatcgacct cgccggcctt gaaggcggcc agggctttca
     1141 gacgctcgtc ctgcgatttg tcgccgtgca aggccgaggt cttgaggccg tcgcgctcga
     1201 aggcacgcgc caggcgggcc gcacccagct tggagttgac gaagacgagg ctctgcgtga
     1261 tgccgcgctc gcgcagcagc tggcgcacgg cgcggcgctt gtcgtcttcc tcgacgcggt
     1321 agaagcgctg ctccacggtc gacgcggtcg cgttcggccg tgccacttcg accagcaacg
     1381 gatcctgaag gtagctttgc gccagcctct tgatttcggg cgaaaaggtg gccgagaaga
     1441 gcaatgtctg acgctgcttg ggcagatagc tcaggatgcg ctgcagatcg ggcaggaagc
     1501 cgatgtccag catgcgatcg gcctcgtcca gcaccacgta ttcgacttgc ccgagcgagc
     1561 agttcctggc ctcgatatgg tcgagcaggc ggccgggcgt ggcgatcagc acctcgacgc
     1621 cggctttgag ctcctgcgtc tgcggcttca tgtcgatgcc gccgaacacg caagccacgc
     1681 gcagctgggt gtgcttggca taggccgtca cattgcgcgc gacctggtcg gccagctcgc
     1741 gcgtgggcgc cagtacaagg gcgcgcaccg ggtggcgggc cggcgaggca ctcgtgttct
     1801 cgtgctttag catgcgctgc aacagaggca gcgagaaggc cgccgtcttg ccggtgccgg
     1861 tctgggcagc gcccatcacg tcacggccgt ccagcacgat ggggatggcc ttggcctgga
     1921 tgggcgtcat cgtgacatag ccggaatcgg ccacggcacg gaggaatttg gcgtcgagtg
     1981 cgagggtgtc gaagcgggcc ggcgcctggt ccgcgatggg gtcagtcatc cacagattat
     2041 ccccgacatc gggaaatcac ttatgaacga ccagggcgcg gggggttttg aagcgcacga
     2101 tgcgctggta gaggaagaca taggtcgcca cgaacagcgc cagaaagccg gtcaggatcg
     2161 ccgagctctg ccaccagaac aggctgggca cgaccgacag cgagcacagc atccagagat
     2221 agggcgaggt catggagttg cggcgcgtca gcacctcggc atcccggttg ccgacggccc
     2281 agcgcatcag ccgacgatag atcagcgtat gcagatgaat cccgtcgggc atgcccatcg
     2341 catggccgcg caccagcttg cggcggtaca tcgaaaagat cgtctcgaag atcgggtagg
     2401 cgcacagcag cagcgggaag atgggcgaga ccgtggggtt gcgatgcacg agcaggacgc
     2461 ccagctcggc cagtatgaag cccaggaaat aggcgccccc gtcgcctagg aagaccaggc
     2521 cgcgcgggta gttccagacg aagaagccca gcaccgcgcc gaccaccgag atcgccaggc
     2581 tcgccaccag ccagtcgccc tcctgcaggg ccacataagc caggcccgaa agcatgatcg
     2641 cggtgcacat cgatgccagc ccgttcatgc cgtcgatgat attgatggag ttggccacgc
     2701 ccgccaccac aaagagcgtg accagcgcag cccctgcagg aaaggcgacc acg
//
LOCUS       DASUCY010000374         4254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_795232, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000374 DASUCY010000000
VERSION     DASUCY010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4254
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795232"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1014)
                     /locus_tag="VFV25_07510"
     CDS             complement(<1..1014)
                     /locus_tag="VFV25_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HEY1393207.1"
                     /translation="MNNQWFSKVAVWLVIALVLFTVFKQFDRTSTAGSQIGYSDFLEE
                     VRAKRIKSVTLQEGGGGTDIKAITTDDKTIRTTATYLDRGLVGDLINNGVKFDVKARE
                     EPSVLMSILISWGPMLLLIGVWIYFMRQMQGGGKGGAFSFGKSRARMLDESNNSVTFA
                     DVAGCDEAKEEVRELVEFLKDPQKFQKLGGRIPRGVLMVGPPGTGKTLLAKAIAGEAK
                     VPFFSISGSDFVEMFVGVGAARVRDMFEQAKKNAPCIIFVDEIDAVGRHRGAGLGGGN
                     DEREQTLNQMLVEMDGFETNLGVIVMAATNRPDILDPALLRPGRFDRQVYVTLPDVRG
                     REQI"
     gene            complement(1190..1864)
                     /locus_tag="VFV25_07515"
     CDS             complement(1190..1864)
                     /locus_tag="VFV25_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEY1393208.1"
                     /translation="MKIKTKSKKVNKAWLHDHMTDPYVKLAQKEGYRARAAYKLKEID
                     EELKLLKPGQLVVDLGAAPGAWSQYVRRKFAPKLAGGGGAAVGELNGSIIALDLLDFE
                     PIEGVSFIQGDFRDESVLQRLQAAVAGRAVDVVISDMAPNLSGVAASDSARIADLVEL
                     AVEFAQNHLKPDGALVCKVFHGSGYSQLVELFKMRFRVVKPIKPKASRDKSAETFLVG
                     IGLKKG"
     gene            1886..2362
                     /locus_tag="VFV25_07520"
     CDS             1886..2362
                     /locus_tag="VFV25_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HEY1393209.1"
                     /translation="MPAIQLTPAERKLHRADAHHLDPVVMIGGEGLTPAVVKETDAAL
                     KAHGLIKVRVFSDERATRDEILAALCEQLDAAPVQHIGKLLVLWRPLPDKPKLEREDR
                     KAGPKVVKILKFSKSGSHRAQVKKLTVLGNQRLTAGGMVKRAKTRQTSTKKRGTPG"
     gene            complement(2370..2807)
                     /locus_tag="VFV25_07525"
     CDS             complement(2370..2807)
                     /locus_tag="VFV25_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HEY1393210.1"
                     /translation="MRGGASVLLPRLRALLPGLWAGMLVFLGAIAAPAPFAVLAPQVA
                     GRVAARMLAGEAYVSLGLGVLLVVIERRLRGRAAPNAEMLLALGALFCTVAGYFALLP
                     MMEAARAGQGALSFAALHAVSSAFFVLKAVLILVLAWRVTRIA"
     gene            complement(2811..3287)
                     /gene="greA"
                     /locus_tag="VFV25_07530"
     CDS             complement(2811..3287)
                     /gene="greA"
                     /locus_tag="VFV25_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEY1393211.1"
                     /translation="MATIPLTHRGAEKLKQELHRLKTVERHAVIAAIAEARAQGDLSE
                     NAEYEAAKDKQGFIEGRILEIEAKLAAAQVIDPATVDADGRVVFGATVELEEESSGDI
                     VTYQIVGEDEADLKLGLISVGSPIARAMIGKEAGEVAEFHAPGGMRRYEIVDVRYV"
     gene            complement(3361..>4254)
                     /gene="carB"
                     /locus_tag="VFV25_07535"
     CDS             complement(3361..>4254)
                     /gene="carB"
                     /locus_tag="VFV25_07535"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857147.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HEY1393212.1"
                     /translation="VHSGDSACSLPPYSLSPALQDELRRQTTLMAKALKVVGLMNVQF
                     AIQGEGDQAVVYVLEVNPRASRTVPYVSKATAQALAKIAARCMAGTRLKDQKGRDGRP
                     PREVIPPYFSIKEAVFPFNKFPGVDPILGPEMRSTGEVMGVGRSFGEAMLKSQLGAGS
                     RLPRKGAVVITVKNSDKARAVKVASDLHDLGFNVLATKGTAAAIAEAGVPVKLVNKVK
                     DGRPHIADMVKAGEVQLVFTTVDETRTAIADSRYIRTAALANRVTYYTTMAGCEAATE
                     ALKHEADLEVYSVQQLHAELH"
BASE COUNT          738 a         1437 c         1341 g          738 t
ORIGIN      
        1 gatctgctcg cggccgcgca cgtcgggcag cgtcacatag acctggcggt cgaagcggcc
       61 ggggcgcagc agcgccgggt cgaggatgtc gggccggttg gtggcggcca tcacgatgac
      121 cccgaggttg gtctcgaagc catccatctc gaccagcatc tggttcaggg tctgctctcg
      181 ctcgtcgttg ccgccgccca ggcctgcgcc gcgatgacga cccacggcat cgatttcatc
      241 gacgaagatg atgcagggcg cgttcttctt ggcctgttcg aacatgtcgc gcacacgggc
      301 cgcgcccacg ccgacgaaca tctcgacgaa atcggagccc gagatcgaga agaaaggcac
      361 cttggcctcg cccgcgatgg ccttggccag cagtgtcttg ccggtgcccg gagggccgac
      421 catcaacacg ccgcgcggga tgcggccgcc cagcttctgg aacttctgcg ggtccttgag
      481 gaactcgacc agctcgcgca cctcttcctt ggcctcgtcg cagccggcca cgtcggcgaa
      541 ggtgacggag ttattgcttt cgtcgagcat gcgcgcccgg ctcttgccga agctgaaggc
      601 gccgcccttg ccgccgcctt gcatctgtcg catgaaatag atccacacgc cgatcagcag
      661 cagcatcgga ccccagctga tgaggatgct catcagcacc gacggctcct cgcgcgcctt
      721 gacgtcgaac ttgacgccgt tattgatgag gtcgccgacc agcccgcggt ccagataggt
      781 cgccgtggtg cggatcgtct tgtcgtccgt cgtgatggcc ttgatgtcgg taccgccgcc
      841 gccttcctgc agcgtgacgc tcttgatgcg cttggcgcgc acttcctcga ggaagtcgga
      901 gtaaccgatc tggctgcccg cggtcgaggt gcggtcgaac tgcttgaaca cggtgaacag
      961 caccagggcg atcaccagcc agacagcaac cttagagaac cattgattgt tcaccgcggc
     1021 tccttcatac gatccaaatg gcgggcatga cctaaagcgg cagggtcgcc cgtgcgttcg
     1081 cggtagttgg ggccaattct agtgcagtca agcccgcgcc cccgtttgat ttttccctcg
     1141 cagggccata gagattcgtg aaattcgaga acactgacgc aaaacaagtt cagcccttct
     1201 tgagaccgat gcccaccagg aaggtttcgg ccgatttgtc gcgcgaagcc ttgggtttga
     1261 tgggcttgac cacgcgaaag cgcattttga agagttcgac cagctggctg tagccgctgc
     1321 cgtgaaagac cttgcagacc agggcgccgt cgggcttgag atggttctgt gcgaactcca
     1381 cggcgagttc caccaggtcg gcgatgcggg ccgaatccga ggcggcgaca ccggacagat
     1441 tcggcgccat gtcggaaatg accacgtcca ctgcacgccc ggccaccgcg gcctgcaggc
     1501 gctgcagcac ggattcatcg cggaagtcgc cctgaatgaa gctcaccccc tcgatgggct
     1561 cgaaatccag caagtcgagc gcgatgatgc tgccgttcag ctcgccgacc gcggccccgc
     1621 cgccgcccgc cagcttgggc gcgaacttgc ggcgcacata ttggctccag gcacccggcg
     1681 cggcgcccag gtcgaccacc aactggccgg gcttgagcag cttcagctcc tcgtcgattt
     1741 ccttgagctt gtaggccgcg cgcgcccggt aaccctcctt ctgggccagc ttcacatagg
     1801 ggtcggtcat atggtcgtgc agccacgcct tgttgacctt cttgcttttt gtcttgattt
     1861 tcatgtgccg tcagataata ggcgtatgcc tgccatacaa ctgaccccgg ccgagcgcaa
     1921 gctgcaccgc gccgatgccc atcatttgga ccccgtcgtg atgatcggcg gcgagggcct
     1981 cacgcccgcc gtcgtcaagg aaaccgatgc ggccctgaag gcccatggcc tgatcaaggt
     2041 gcgcgttttc tccgatgaac gcgcaacccg cgacgaaatc ctcgccgcct tgtgcgagca
     2101 gctcgacgcc gccccggtgc agcacatcgg caagctgctg gtgctgtggc ggccgctgcc
     2161 ggacaaaccc aagctcgagc gcgaggaccg caaggccggc cccaaggtgg tcaagatact
     2221 caaattctcc aagagcggca gccatcgcgc ccaggtcaag aaactcaccg tgctgggcaa
     2281 tcaacggctg accgccgggg gcatggtcaa gcgggccaag acgcggcaga cgagcaccaa
     2341 gaagcgcggc acgcccggtt gaggccgcat caggcgatac gggtgacgcg ccaggccagg
     2401 accaaaatca gaacggcctt gagcacgaaa aaggccgatg acaccgcatg caaggctgcg
     2461 aacgatagcg ccccctgccc ggccctcgcc gcctccatca tcggcaacag cgcgaagtag
     2521 ccggccactg tgcagaacag ggctccgagc gccagcagca tctccgcatt cggggcggcc
     2581 cggccgcgca ggcgccgctc gatcacgacc agcagcacgc ccaggcccag gctgacatag
     2641 gcttcgccgg ccagcatgcg ggcggccacg cgcccggcca cctgcggcgc gaggacagca
     2701 aacggcgccg gagctgcaat ggcgcccagg aaaacgagca tgccggccca caggccgggc
     2761 aatagcgcgc gcaggcgcgg cagcaacacg ctggctccgc ctctcatctg tcagacgtag
     2821 cgaacgtcga cgatctcgta gcggcgcatg ccgcccggcg cgtggaactc ggccacttcg
     2881 ccggcctcct tgccgatcat cgctctcgcg atcggcgaac cgaccgatat caggcccagc
     2941 ttcaagtcgg cctcgtcctc gccgacgatc tgataggtaa cgatatcgcc cgaagactcc
     3001 tcctcgagct ccacggtcgc accgaacacc acgcgtccat cggcgtccac cgtcgccggg
     3061 tcgatcacct gggcggcagc caacttggct tcgatctcca ggatgcgacc ctcgatgaac
     3121 ccttgcttgt ccttggccgc ttcgtactcg gcgttctccg acaggtcgcc ctgggcgcgc
     3181 gcctcggcga tcgcagcgat gacggcgtgg cgctcgaccg ttttcagtct gtgcagctcc
     3241 tgcttgagct tttcggcgcc gcggtgggtc agcggtatgg tggccatgga atcctcgggc
     3301 aaaaaaaatc cagccgccac cagttgcctg gcggcggccg tgggtcgaac aggcgctagt
     3361 ttagtgcagt tcggcgtgca attgctgcac ggagtacacc tccagatcgg cttcgtgctt
     3421 gagtgcctcg gtggcggcct cgcagccggc catggtcgtg tagtaagtca cgcgattggc
     3481 cagggccgcc gtgcggatgt agcgcgaatc ggcgatcgcc gtgcgcgtct cgtcaacggt
     3541 ggtgaatacg agctggacct cgccggcctt caccatgtcc gcgatatggg ggcgcccgtc
     3601 cttgaccttg ttgaccagct tgaccggcac gccggcctcg gcgatcgccg ccgccgtgcc
     3661 cttggtggcc agcacgttga agcccaggtc gtgcaggtcg gacgcgacct tgacggcgcg
     3721 cgccttgtcg ctgttcttga ccgtgatgac cacggcgccc ttgcgcggca gccgcgagcc
     3781 ggcgccgagc tggctcttga gcatggcttc gccgaagctt ctccccacgc ccatcacctc
     3841 gccggtggag cgcatctcgg gacccaggat gggatcgacg cccgggaact tgttgaacgg
     3901 gaagacggct tccttgatgc tgaagtaagg cgggatcacc tcgcgcggtg ggcgaccgtc
     3961 cctgcccttc tggtccttca gccgggtgcc ggccatgcag cgcgcggcga tcttggccag
     4021 cgcctgggcg gtggccttgg agacgtaggg cacggtgcgc gaagcacgcg ggttcacttc
     4081 cagcacatag accaccgcct ggtcgccttc gccctggatc gcgaactgca cgttcatcag
     4141 gcccaccacc ttcagcgcct tggccatcag cgtggtctgg cggcgcagct cgtcctgcag
     4201 cgccggcgac agcgagtacg gcggcaatga gcatgccgag tcgccggagt gcac
//
LOCUS       DASUCY010000375         6074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_425947, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000375 DASUCY010000000
VERSION     DASUCY010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 6074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6074
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425947"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1094
                     /locus_tag="VFV25_07540"
     CDS             <1..1094
                     /locus_tag="VFV25_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393213.1"
                     /translation="LIVYELQPALNFWGRLWQRLKAFFLHWHGPRRDRDPTFADRLAA
                     DEMYWSKALGRAEVRDKASVIQRRGALLAPASAPAVQYRPAGGEGRSAHVVLVHGGLS
                     SVRNAFEAWLAPQATEQAPLWPGMPIFDAACVWRFEHDSFLSLDKNIAALCEAMRSRV
                     VGNAAGGGRIVFLTHSRGGAVVRFALPQLQASWPQWSFHALTAGAPHDGTPVFGQLGQ
                     RWVGLASLVGVLGRAADGLLDREQMAKLKNLERGLAGDIPPGFDDVTPQNIRLRARGE
                     EFPVGIETWGSHWRADFADDWSDQEWHHVVEGVVGLEADGDGMIPLNSAKMGPVQNDA
                     SPAFHTEYFHHRVAVAAMRARIEALLSKN"
     gene            1094..2029
                     /locus_tag="VFV25_07545"
     CDS             1094..2029
                     /locus_tag="VFV25_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013206193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEY1393214.1"
                     /translation="MKPPPIALAIHGGAGTIRRSEMTAEREAAYRAGLQAALDAGHSV
                     LRSGGAALEAVQASVLALEDDPLFNAGRGAVFNDQGYNELEAAIMDGVTRAAGSVTGL
                     RRVRNPVLLARAVMERTPHVTLGFEAAEAYAAALGLALEPAEYFRTEARWQALQSELA
                     RLRAGGSDTEVSEAVKHGTVGAVALDTQGRLAAATSTGGRTAKWAGRVGDTPVIGAGT
                     WADERVAVSGTGHGEWFVRCAVAHDIAARMRHGASLSEACDQVVMGELVRYGGSGGVI
                     AVDAAGHVAMPFNCEGMYRAAVDAEGRCSVAIFGA"
     gene            complement(2059..3606)
                     /locus_tag="VFV25_07550"
     CDS             complement(2059..3606)
                     /locus_tag="VFV25_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA synthase"
                     /protein_id="HEY1393215.1"
                     /translation="MTSSSDDDNNNLYAALRAGFPAHLDGWAIETDGGLVYTWRDIDR
                     ATAMLANLLASLKLPAGSRIAAHTDKSVEALLLYLATLRAGHVYLPLNPAYQQAELEY
                     FIADAEPAVVVCASRNFPWLSKLAFKADVRHVFTLDDDRSGSLLERASHMSDHHEVLA
                     SSTDDLAAILYTSGTTGRSKGAMLSHGNLLSNARTLRDYWDWQPGDVLIHALPIFHVH
                     GLFVAAHGALLNGSPMLWLSRFEPKAVIERLPRATVFMGVPTLYVRMLAEPGFTAGAC
                     RNMRLFVSGSAPLLIDTFGDWQERSGHTILERYGMSETVMLSSNPCRSTDGERRGGTV
                     GKPLPGVQLRVVGERGQPLPPDEVGDIEVKGPNVFSGYWRMPEKTAEEFTSDGWFKTG
                     DVGRFDADGYLTIVGRSKDLIISGGFNVYPAEVEGYLNEMEGVAESAVVAAPHPDFGE
                     AVVAIVVPKPGAALDEDELIGQLKRQIASYKVPKRLFVVTELPRNAMGKVQKKLLREQ
                     HQSLFGG"
     gene            complement(3609..4400)
                     /locus_tag="VFV25_07555"
     CDS             complement(3609..4400)
                     /locus_tag="VFV25_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY1393216.1"
                     /translation="MTGEVRFERDPQQPAIGLLTLAHPAKLNAISLAMWRALRELAEG
                     FDAAAPALAVVILRGEGEAFAAGADIAEFPQFRFEATSLRDYHEAVIAPALQALLATN
                     VPLIAQITGACVGGGLEIASCCDIRIADASARFGAPIAKLGFPMAPDELAVVLAAAGH
                     ATAAELLLEARLIDAATALQRGLLHRVADDAQAESLATARRIAALPVDVARANKRTLR
                     QLRRGTLGAAERAAHFGYAAAGYHREGIAAFLEGRAPDFTAWNQT"
     gene            4481..4912
                     /locus_tag="VFV25_07560"
     CDS             4481..4912
                     /locus_tag="VFV25_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HEY1393217.1"
                     /translation="MFAPSQLEVRQFFCEAFRKSRAAEPLTPLEALAADWISQHPEYD
                     ADLADVEAAMNASYTVEEGRSNPFLHLAMHLSISEQVSIDQPRGIKQAVELLAHRRGS
                     LHAAQHEVMECLGEMMWASQRSGLPPDGEAYIDAVRRRATR"
     gene            complement(4934..>6074)
                     /locus_tag="VFV25_07565"
     CDS             complement(4934..>6074)
                     /locus_tag="VFV25_07565"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY1393218.1"
                     /translation="VLDEQGRAIAGDPGLAAMPVSVAPGGWRLVDTSMEGVPIRMAVR
                     GLECGTRNCQVRMAETRNKREQLNAQVLRGGLALIFGVVVLLAGVVWWVVVKALRPLQ
                     ALRLQMASRTLDDLPPLDPGRVPAEVYPLVQAINELFERLRAAAQAQQAFIADAAHQL
                     RTPLTALRTETELALLEPHPGELQPTLNRLHNAAERAARVADQLLALARTEAAVRQSR
                     RAEVFDLKILAQQAAQDWVPRALQRGVDLGFQLESARVQGRSYLVRELLANLIHNALE
                     YTPPHAGERARVTVRTRVEPEGFAVLEVEDNGPGIPAEEHELVFERFHRGAGAPGPGS
                     GLGLAIVRDIALAHGARVSLAAADEDGRGLKVKVIWRIAGSKAAQ"
BASE COUNT          919 a         2051 c         2160 g          944 t
ORIGIN      
        1 cgctgatcgt ctatgagctt cagcctgccc tgaacttctg gggccggctg tggcagcggc
       61 tgaaggcctt ttttttgcac tggcatggcc cgcgccgcga tcgtgatccg accttcgccg
      121 accgattggc ggccgatgag atgtactgga gcaaggccct gggccgggcc gaagtgcggg
      181 acaaagccag cgtcatccag cgtcgcggcg ccttgctggc accggcctcc gccccggccg
      241 tgcaatacag gccggcgggc ggagaaggac gcagcgccca tgtcgtgctt gtgcatggcg
      301 gcctgagctc ggtacgcaat gctttcgagg cctggctcgc accgcaggcc acggagcagg
      361 cccccttgtg gccgggcatg cccatcttcg atgcggcctg cgtctggcgc ttcgagcacg
      421 acagtttcct gtcgctggac aagaacatcg cagccctgtg cgaggccatg cgcagccggg
      481 tggtgggcaa cgcggcgggc ggcgggcgca ttgtgtttct cacgcattcg cgcggcggcg
      541 cagtggtgcg cttcgccttg ccgcaactgc aggcgagctg gccgcagtgg agcttccacg
      601 ccctcacggc cggggctccg catgacggta cgccggtatt cggccaacta ggtcagcgct
      661 gggtaggcct ggccagcttg gtcggcgtgc ttggacgcgc cgcagatggc ctgctggacc
      721 gcgagcagat ggccaagctc aagaacctcg agcgcggcct cgccggcgac atcccgcccg
      781 gcttcgacga cgtcacgccg cagaacatcc gcctgcgcgc gcggggcgag gagtttcccg
      841 tcggaatcga gacctggggc tcgcactggc gcgccgattt cgccgacgat tggtccgacc
      901 aggaatggca ccacgtggtc gaaggcgtgg tcggcctgga agccgacggc gacggcatga
      961 ttccgctgaa ttcagcgaag atggggccgg tgcagaacga cgcctcgccg gcctttcaca
     1021 ccgagtattt ccatcaccgg gtcgccgtcg ccgcgatgcg cgcgcggatc gaggcgttgc
     1081 tgagcaaaaa ctgatgaagc ctcctcccat tgccctggcc atccacggcg gcgccggcac
     1141 catacggcgc agcgagatga cggccgagcg cgaagcggcc taccgcgccg ggctgcaggc
     1201 tgcgctcgat gcggggcact ccgtgctgcg ctcgggcggt gctgcgctcg aagccgtgca
     1261 ggcctcggtg ctcgcgctcg aggacgatcc gctcttcaat gccggtcgcg gcgcagtctt
     1321 caacgaccag ggctacaacg agctggaggc cgccatcatg gacggcgtga cgcgcgcggc
     1381 cggcagcgtc accgggctgc gccgcgtgcg caacccggtg ctgctcgcgc gcgcggtgat
     1441 ggagcgcacg cctcatgtga cgctgggctt cgaggcggcg gaagcctacg cggccgcact
     1501 cggcctggcg ctggagccgg ccgagtattt ccgcaccgag gcgcgctggc aggcgctgca
     1561 aagcgagctg gcacgcctgc gcgccggcgg cagcgatacg gaggtgtcgg aagcggtgaa
     1621 gcacggcacg gtcggtgcgg tggcgctcga tacccagggc cggctcgccg cggcgacctc
     1681 caccggtggg cgcaccgcca agtgggccgg gcgcgtcggc gacacgccgg taatcggtgc
     1741 cggcacctgg gccgacgagc gcgtcgccgt ctcgggcacc ggccacggcg aatggttcgt
     1801 tcgttgcgcg gttgcgcatg acatcgccgc tcgcatgcgg cacggtgcga gcctcagcga
     1861 ggcctgcgac caggtcgtga tgggcgagct ggtgcgctac ggcggcagtg gcggcgtcat
     1921 cgccgtcgat gcggccggcc atgtcgcgat gcccttcaat tgcgagggga tgtaccgggc
     1981 ggcggtcgat gccgaggggc ggtgtagcgt cgcgatcttc ggtgcttgat gcccggagcc
     2041 gcgatccgct aagcggcgtc agcctccgaa caggctctga tgttgctccc gtagcagctt
     2101 cttctgcacc ttccccattg cattgcgcgg caactccgtg acgacaaaga gccgcttggg
     2161 caccttgtag ctcgcaatct ggcgcttgag ctggccgatc agctcgtcct cgtccagcgc
     2221 cgcacccggc ttgggcacga cgatggccac cacggcctcg ccgaagtcgg gatgcggggc
     2281 cgcgaccacg gcagattcgg caacgccttc catctcgttg agatagccct ccacctcggc
     2341 cgggtagacg ttgaagccgc cgctgatgat caggtccttg ctgcggccga cgatggtgag
     2401 atagccgtcg gcatcgaagc ggcccacgtc gccggtcttg aaccagccgt cgctggtgaa
     2461 ttcctcggcc gtcttctccg gcatgcgcca atagcccgag aagacattcg gccccttgac
     2521 ctcgatgtcg ccgacctcgt ccggcggcaa aggctggccg cgctcaccga ctacgcgcag
     2581 ctgcacgccg ggcagcggct tgccgaccgt gccgccgcgg cgctcgccgt cggtgctgcg
     2641 gcaagggttg gagctcagca tcacggtctc gctcatgccg tagcgttcga ggatggtgtg
     2701 gccgctgcgc tcctgccagt cgccgaaggt atcgatcagg agcggcgccg agcccgagac
     2761 gaagagccgc atgttgcggc acgcgccggc cgtgaagcca ggctcggcga gcatgcgcac
     2821 atagagcgtg ggcacgccca tgaacacggt ggcacgaggc agccgctcga tgacggcctt
     2881 gggctcgaag cgcgacagcc acagcatcgg gctgccattg agcagcgccc cgtgcgcggc
     2941 gacgaagagg ccgtgcacgt ggaagatcgg tagcgcatgg atcagcacgt cgccgggctg
     3001 ccagtcccag tagtccctca gcgtgcgcgc attgctgagc agattgccgt ggctgagcat
     3061 cgcgcctttg ctgcggccgg tcgtgcccga ggtgtagagg atggcggcca gatcgtcggt
     3121 ggaactggca agcacctcgt ggtgatcgct catgtggctg gcgcgttcca gcagcgagcc
     3181 gctcctgtcg tcgtccagtg tgaacacgtg gcgcacgtcc gccttgaagg ccagcttgct
     3241 gagccagggg aaattgcgcg aggcgcacac caccacggcc ggttcggcgt cggcgatgaa
     3301 atattccagc tcggcctgtt gataggccgg gttgagcggc agataaacat ggcccgctcg
     3361 cagcgtcgcg agatagagca gcagcgcttc gaccgacttg tcggtgtggg ccgcgatgcg
     3421 cgagccggcc ggcagcttga gcgaggccag cagattggcc agcatcgccg tcgcgcgatc
     3481 gatgtcgcgc caggtgtaga cgaggccgcc gtcagtttct atcgcccagc cgtcgagatg
     3541 ggccggaaag ccagcgcgca gggcggcata gaggttgttg ttgtcgtcgt cgctgctgct
     3601 cgtcatcgtc atgtctggtt ccaggccgtg aagtcgggcg cacgtccctc gaggaaggcg
     3661 gcaatgcctt cgcgatggta gccggcggcg gcatagccga aatgcgccgc gcgctcggcg
     3721 gcgcccagag tgccgcggcg caattggcgc agcgtgcgct tgttggcccg agccacgtcg
     3781 acgggcaggg ccgcgatgcg gcgggccgtg gcgagcgact cggcctgcgc gtcgtcggcg
     3841 acgcggtgca gcaggccacg ctgcaaggcg gtggccgcgt cgatcagcct tgcctcgagc
     3901 agcagttcgg cggccgtggc gtggccggcg gccgccagca cgacggccag ctcgtccggc
     3961 gccatcggaa agcccagctt cgcaatcggc gcgccgaagc gggccgaggc atcggcaatg
     4021 cggatgtcgc agcagctggc gatctccagc ccgccgccga cgcaggctcc ggtgatctgc
     4081 gcgatcagcg gcacattggt cgcgagcagg gcctgtagcg ccggcgcgat gacggcctcg
     4141 tggtagtcgc gcaggctcgt ggcctcgaag cggaactgcg ggaactccgc gatatcggcc
     4201 ccggccgcga aggcttcgcc ctcgccgcgc aggatcacca cggccagcgc aggtgcggct
     4261 gcatcgaaac cttccgccag ctcgcgcaag gcgcgccaca tcgcgaggct gatggcattg
     4321 agcttggccg gatgcgccag cgtcagcagg ccgatggccg gctgttgcgg atcacgctcg
     4381 aagcggacct cgcccgtcat gcggccggcc catccattct tgactgctgg ctgacatcca
     4441 tgtagcgggc ctttcacaaa agcggggcga caattgtcga atgtttgccc ccagccaact
     4501 cgaagtccga cagttcttct gcgaggcctt ccgcaagagc cgcgccgccg agcccttgac
     4561 gccgctggaa gccctggcgg ccgactggat cagccagcat cccgaatacg acgcagacct
     4621 ggccgatgtc gaggccgcga tgaatgcgag ctatacggtc gaggaaggcc gcagcaaccc
     4681 cttcctgcac cttgcgatgc atttgtcgat cagcgagcag gtcagcatcg accagccgcg
     4741 cggcatcaag caggcggtgg agttgctcgc ccatcgccgc ggctctctgc acgcggccca
     4801 gcatgaggtg atggaatgcc tgggcgagat gatgtgggcc tcgcagcgca gcggcctgcc
     4861 gccggacggc gaggcctata tcgacgcggt cagacggcgg gcgacacgct gaggctgccg
     4921 ccgcaaagga cgctcactgg gccgccttcg agcccgcgat cctccagatc accttcacct
     4981 tcagtccccg gccgtcctcg tccgccgcgg ccaggctgac cctggcgcca tgggccagcg
     5041 caatgtcgcg cacgatggcc aggcccaggc cgctgccggg gccgggggcg ccggcgccgc
     5101 gatgaaagcg ctcgaacacg agttcgtgct cttccgcggg aatgccgggg ccgttgtctt
     5161 cgacctccag gaccgcgaag ccttcgggtt ccacgcgggt gcgcaccgtc acccgggcac
     5221 gctcgccggc atggggcggg gtgtactcca gcgcgttgtg gatcaagttg gccaacagct
     5281 cccgcaccag gtaggaacgg ccctgcacgc gcgcgctttc gagctggaag cccaggtcga
     5341 cgccgcgctg cagggcacgc ggcacccagt cctgggcggc ctgctgggcc aggatcttga
     5401 gatcgaagac ttcggcgcgc cggctctggc gcaccgcggc ctcggtacgc gccagcgcca
     5461 gcagctggtc ggccacgcgc gccgcgcgct cggcagcgtt gtgcaggcgg ttgagcgtgg
     5521 gctgcagctc gccggggtgg ggttcgagca gggccagttc ggtctcggtg cgcagcgcgg
     5581 tgagcggggt gcgcagctgg tgcgcggcat cggcgatgaa ggcctgctgg gcctgggccg
     5641 cggcgcgcag ccgctcgaag agctcgttga tggcctgcac cagcggataa acctcagccg
     5701 gcacacgccc ggggtcgagc ggcggcaggt cgtccagcgt gcggcttgcc atctgcaggc
     5761 gcagggcttg cagcggacgc agggccttca cgaccaccca ccacacgacc ccagcgagga
     5821 gcacgaccac gccgaagatc agcgccaggc cgccgcgcag cacctgggca ttgagctgct
     5881 cgcgcttgtt gcgggtttcc gccatgcgca cctggcagtt cctggtgccg cattcgaggc
     5941 cgcgcaccgc catgcggatg ggcacccctt ccatgctcgt gtcgaccagg cgccagccgc
     6001 ccggcgccac gctgaccggc atggccgcga ggcccggatc gccggcgatg gctcggccct
     6061 gttcgtccag cacg
//
LOCUS       DASUCY010000376         9518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_136556, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000376 DASUCY010000000
VERSION     DASUCY010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 9518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9518
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136556"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..138
                     /locus_tag="VFV25_07570"
     CDS             <1..138
                     /locus_tag="VFV25_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEY1393219.1"
                     /translation="DWAYTSGDKMAADLTYVALPDSVKALIRKEWGDIKDGSGKTVAF
                     N"
     gene            300..1259
                     /gene="pstC"
                     /locus_tag="VFV25_07575"
     CDS             300..1259
                     /gene="pstC"
                     /locus_tag="VFV25_07575"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HEY1393220.1"
                     /translation="MGRPPVARGSAPWADMVFSFLALSAAVLTLALLVAIMVSLFIGA
                     TPAIKEYGLSFLWRSQWDPVQRQFGGLVPIYGTLMTSFIALLIGVPVSFGIALFLTEL
                     SPAWLKRPLGIAIELLAAIPSIVYGMWGLLVFGPILAEYVQQPLQSLLGDVPGLGALV
                     SGPPVGLGLLSAGIILAIMVIPFIASVMRDVFEVTPPMLKESAYGLGSTTWEVVFRVV
                     LPYTKTGVIGGIMLGLGRALGETMAVTFVIGNMNQLNSISLFEAANSITSALANEFAE
                     AGEGLHQASLIYLGLILFFITFVVLALSKLLLNQLKKGEGARS"
     gene            1256..2116
                     /gene="pstA"
                     /locus_tag="VFV25_07580"
     CDS             1256..2116
                     /gene="pstA"
                     /locus_tag="VFV25_07580"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEY1393221.1"
                     /translation="MSNMDGTRLARHRARKRTNAIALTLSLAAMAFGVFWLIWILLET
                     VHLGLDGLAWSTFTQMTPAPQADGGGLANAIFGSLMMVGLATCIGAPVGILAGIYLAE
                     YGQKTWLGAATRFINDILLSAPSIVIGLFIYTVVVARVKSFSGWAGVLALALLVIPVV
                     IRTTENMLSLVPHALREAAYALGTPKWKVILSITLKAARAGVITGVLLAVARIAGETA
                     PLLFTALSNQFWTSSLSQPMASLPVTIFKFAMSPYENWQKLAWAGVFLIAFSVLLLNI
                     LARSFLRNKH"
     gene            2146..2931
                     /gene="pstB"
                     /locus_tag="VFV25_07585"
     CDS             2146..2931
                     /gene="pstB"
                     /locus_tag="VFV25_07585"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEY1393222.1"
                     /translation="MDAKVELPLNRDAKLSARGLNFYYGHFHALKNINLDIPDRKVTA
                     FIGPSGCGKSTLLRTFNRMFELYPEQRAEGQILLDGEDILTTRQDVSLIRAKVGMVFQ
                     KPTPFPMSIYDNIAFGVRLFESLPRVEMDERIEWALKKAALWSEVKDKLHQSGAGLSG
                     GQQQRLCIARGIAIKPEVLLLDEPCSALDPISTGKIEELIHELKNDYTVVIVTHNMQQ
                     AARCSDYTAYMYLGDLIEFGPTSDLFMKPKKKDTEDYITGRFG"
     gene            2952..3659
                     /gene="phoU"
                     /locus_tag="VFV25_07590"
     CDS             2952..3659
                     /gene="phoU"
                     /locus_tag="VFV25_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEY1393223.1"
                     /translation="MTDKHLSTQFDAELSGISTRVLEMGGLVESQVEQAIYALTNFSA
                     ETAAQVLTQEERVNQMEVEIDHDLSTIIARRQPTARDLRLLMAISKTTANLERVGDEA
                     ARIARTVQKHVSSGVSGRLKLPVSDVSFEADLATASLRKALDAFARLDTAKALEVIKQ
                     DDQIDEEFDGMMRKLITYMMEDPRTISASIDLVFVAKAIERVGDHAKNLAEQIIYIVK
                     GTDVRHNPVETVESMVR"
     gene            3694..4395
                     /gene="phoB"
                     /locus_tag="VFV25_07595"
     CDS             3694..4395
                     /gene="phoB"
                     /locus_tag="VFV25_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HEY1393224.1"
                     /translation="MSRILVVEDESAIAELISINLRHAGYEVTVAITADQAQLEVDRV
                     LPDLVILDWMLPGQSGLALARRWRGEARTKELPIIMLTARADEGDKVAGLDAGADDYL
                     TKPFSTNELKARIRAVLRRKAPEALDSAVELGGLRLDPATRRVARGDQEVKIGPTEFR
                     LLHFLMTHPERVHSRAQLLDRVWGDHVFIEERTVDVHVKRLREALLPANCSQMIETVR
                     GAGYRLTQQSQALSA"
     gene            4442..5821
                     /gene="phoR"
                     /locus_tag="VFV25_07600"
     CDS             4442..5821
                     /gene="phoR"
                     /locus_tag="VFV25_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HEY1393225.1"
                     /translation="MTWFFPRIAMRVLTLLAGGLLGDLLGGVFNAATPGLLLGVALAV
                     SALLIFDMLRGYRLINWLRGPQEQPAPRDTGFWGEMAYRVERAIRVRELNAEQEQTRL
                     AQFLSAIEASPNGVVLLDVNDQIDWCNSVAADHFGLDPLRDRRQRITNLVRAPAFVVY
                     LQLGRFDDPVTFPVARGRGTLSVLVRRYGEGKKLILSQDITERERNDAMRRDFVANVS
                     HEIRTPLTVLAGFIETMSSLPLTEVERRRVLTLMTQQTQRMQTLVSDLLLLAQLEGSP
                     RPSADRWVAVSKLLSHVESDATTLSQGRHKLSFGGAPGQDLSAAQIAGSESELLSALA
                     NLVNNAVRYTPEGGQIDVVWRLRDDGSGEFAVIDTGIGIDKEHLPRLAERFYRVDGSR
                     SRDTGGTGLGLSIVKHVAQRHGGELSITSEPGKGSTFRIVFPASRVRYIEPATDPRRE
                     SAETVAGAR"
     gene            5960..6958
                     /gene="bioB"
                     /locus_tag="VFV25_07605"
     CDS             5960..6958
                     /gene="bioB"
                     /locus_tag="VFV25_07605"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEY1393226.1"
                     /translation="MSQHPVQLVRRPPPVASTASSGTQRWSVDAVAALFELPFNDLLW
                     RAQQTHREHFDANEVQRSTLLSIKTGGCPEDCGYCPQSVHYDTGVEAGKLMDVQAVVE
                     AAQAALNAGATRFCMGAAWREPKDRDIEKVAELVREVKALGLETCCTLGMLSAPQAQA
                     LKVAGLDYYNHNLDTAPEAYGRIISTRDYQERLDTLAHVREAGMSVCCGGIVGMGESR
                     RERAGLIAQLANLEPYPESVPINELVQVEGTPLHGTDKLDPLEFVRTIAVARITMPKA
                     RVRLSAGRQQMSEAVQALCFLAGANSIFYGEKLLTTGNPDVARDEALFEKLGLHAA"
     gene            6974..7441
                     /locus_tag="VFV25_07610"
     CDS             6974..7441
                     /locus_tag="VFV25_07610"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY1393227.1"
                     /translation="MTAVIIRHLAKHQEVLPVLQAWFESEWPAHYGPGGRGDARQDLL
                     AYSNAGSVPIGFVAFRGGVPCGFAALKREAFATHPQLSPWAGAAYVEPALRRQGIGRA
                     LVASLEAEARRLGYPRIYGATSTAGSLLERCRWSLLESVVHDGEQVAVYEKAL"
     gene            7445..8722
                     /locus_tag="VFV25_07615"
     CDS             7445..8722
                     /locus_tag="VFV25_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HEY1393228.1"
                     /translation="MSARKVALIGAGPAGLMAAEVLAQHEGIQVDLYDAMPSSGRKFL
                     LAGKGGLNLTHSEPIEPFLGRYAARRAQVEPWLREFGPEDLRAWAQGLGVATFVGTSG
                     RVFPTDMKAAPLLRAWLHRLRESGVRLHMRHRWLGWAEDGRALRFATPQGEQAIEADA
                     VLLALGGASWARLGSDGAWVPWLRDRGVEVATLAPANCGFDVGQGAQAVEGRPAQGWS
                     EHFRSRHAGEPLKGMGLRFVNADGGAHFERAGEFIITEGGVEGSLIYAASALLRDEIA
                     ARGEAVIQLDLLPARDAAWVRTEVAHPRGSRSMSSHLQSRLGLHGAKAALLRELLPAE
                     AFQRPGALAAAIKALPLRLVAPRPVDEAISSAGGVRFEALDAELMLKALPGVYCAGEM
                     LDWEAPTGGYLLTACFASGRAAARGVLRHLGRD"
     gene            complement(8730..9446)
                     /locus_tag="VFV25_07620"
     CDS             complement(8730..9446)
                     /locus_tag="VFV25_07620"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HEY1393229.1"
                     /translation="MKRRLFFFLFALALACGLGAARAQSPEGRKFVPGPSFDAIEISG
                     SAVVRFTQGASDQVLIEGDQGVQDTVDVEVRNGTLSINQSGAWKFWSARRLQINVTAR
                     NLKRVAISGSADFVAPQPVNVELLTIDISGAGTARFDRLKAEQLVFQVSGAGDGQMAG
                     EVKSLSLRIAGKSNFQGAKLMSEQAKVAISGVGNVGVWVSRELDVSIAGIGTVDYWGN
                     PPVVKRSISGRADINDRGAK"
BASE COUNT         1525 a         3211 c         3193 g         1589 t
ORIGIN      
        1 gactgggctt atacgagcgg cgacaagatg gcagccgacc tgacctatgt ggccctgccc
       61 gacagcgtga aggcgctgat ccgcaaggaa tggggcgaca tcaaggatgg ttccggcaag
      121 accgtcgcgt tcaactaaga aacccatcgc ctggccggcg ccccgccggt cgggctgatc
      181 acggggctgc tcgagagggc agcccttcag cgtatgtagt cggagggatc gtggccgcca
      241 cacttcctgc cagcccctac aaagaagaac aacagcagaa gatcgatcgc tccggcccca
      301 tgggccgccc gccagtcgcg cgcggttcgg cgccttgggc cgacatggtg ttctccttcc
      361 tggccctctc ggcagccgtg ctgacgctgg cgctgctggt cgccatcatg gtgtcgctct
      421 tcatcggcgc gacgcccgct atcaaggaat acggcctgag cttcctgtgg cgcagccaat
      481 gggatccggt gcaacgccag ttcggcggcc tggtgccgat ctacggcacg ctgatgacct
      541 ccttcatcgc gctgctgatc ggggtcccgg tgagcttcgg catcgcgctc ttcctcaccg
      601 agctgtcgcc cgcctggctc aagcgcccgc tgggcatcgc gatcgagctg ctggcggcga
      661 tcccctccat cgtctacggc atgtggggcc tgctcgtgtt cggccccatc ctggcggaat
      721 acgtgcagca gccgctgcag tcactgctcg gcgacgtgcc cgggctcggc gcgctggtca
      781 gcggcccgcc ggtggggcta ggcctgctgt ccgccggcat catcctggcc atcatggtga
      841 ttcccttcat cgcctcggtg atgcgcgacg tgttcgaggt cacgccgccg atgctgaagg
      901 aatccgccta cggcctgggt tccacaacct gggaagtcgt gttcagggtc gtgctgccct
      961 ataccaagac cggcgtgatc ggcggcatca tgctgggcct gggtcgcgcc ctgggcgaga
     1021 ccatggcggt caccttcgtg atcggcaaca tgaaccagct gaactcgatc tcgctcttcg
     1081 aagccgccaa cagcatcacc tcggcgctgg ccaacgagtt tgccgaggcc ggcgaagggc
     1141 tgcaccaggc ctcgctgatc tacctgggtc tgatcctgtt cttcatcacc ttcgtcgtgc
     1201 tggccctgtc caagctgctg ctgaaccagc tcaagaaggg cgaaggagcg cggtcatgag
     1261 caatatggac ggcacccgcc tggcgcggca ccgcgcgcgc aagcgcacca atgcgatcgc
     1321 gctgacgctg tcgctggccg cgatggcctt cggcgtgttc tggctgatct ggatcctgct
     1381 cgaaacggtg cacctggggc tggacggcct ggcctggtcc accttcacgc agatgacgcc
     1441 ggccccgcag gccgacggcg gcggcctggc caatgcgatc ttcggctcgc tgatgatggt
     1501 cggactggcc acctgtatcg gcgcgccggt gggcatcctg gccggtatct acctggccga
     1561 atacgggcag aagacctggc tgggcgccgc cacgcgcttc atcaacgaca tcctgctgtc
     1621 ggcgccgtcc atcgtgatcg gcctcttcat ctacacggtg gtggtggcgc gggtgaagtc
     1681 gttctcgggc tgggcgggcg tgctggcgct ggcgctgctc gtgatccccg tcgtgatccg
     1741 caccaccgag aacatgctca gcctggtgcc ccacgcgctg cgcgaggccg cttatgcgtt
     1801 gggcacgccc aagtggaagg tgatcctgag cattacgctc aaggcggcgc gtgcgggcgt
     1861 catcaccggc gtgctgctgg cggtggcgcg catcgccggc gagacggcgc cgctgctgtt
     1921 cacggcgctg tccaaccagt tctggacctc cagcctgagc cagcccatgg ccagcctgcc
     1981 ggtgacgatc ttcaaattcg cgatgagccc ctatgagaac tggcagaagc tggcctgggc
     2041 gggggtgttc ctgatcgcct tcagcgtgct gctgctcaac atcctggcgc gcagcttcct
     2101 gcgcaacaag cattgattga tcatcgatcg accgacgaga tacccatgga cgccaaagtc
     2161 gaattgccgc tgaacaggga cgccaagctc tcggcgcgcg ggctcaactt ctactacggg
     2221 catttccatg ccctgaagaa catcaacctc gacatcccgg atcgcaaggt cacggccttc
     2281 atcgggccat cgggttgcgg caagtccacc ctgctgcgca ctttcaaccg catgttcgag
     2341 ctctatcccg agcagcgcgc tgaaggccag atcctgctgg acggcgaaga catcctgacc
     2401 accaggcagg acgtctcgct gatccgtgcc aaggtcggca tggtcttcca gaagcccacg
     2461 cccttcccga tgtcgatcta cgacaacatc gccttcggcg tgcgcctgtt cgagtcgctg
     2521 ccaagggtcg agatggacga gcgcatcgaa tgggcgctga agaaggcggc gctgtggagc
     2581 gaggtcaagg acaagttgca ccaaagcggc gccggcctgt ccggcggcca gcagcagcgc
     2641 ctgtgcatcg cgcgcggcat cgccatcaaa cccgaagtgc tgctgctgga cgagccctgc
     2701 tcggcgctgg atcctatttc gactggcaag atcgaagaac tgatccacga gctgaagaac
     2761 gactacacgg tggtcatcgt gacgcacaac atgcagcagg ctgcccgctg ctcggactac
     2821 acggcctaca tgtatctggg cgacctgatc gagttcgggc cgacctcgga cctcttcatg
     2881 aagccgaaga agaaggacac cgaagactac atcactggaa gattcggctg aggaccaaag
     2941 ggggcaatgc catgacggac aagcatctat cgacccagtt cgatgccgaa ctgagcggca
     3001 tctcgacccg cgtgctggaa atgggtgggc tggtggagtc ccaggtggaa caggcgatct
     3061 atgcgttgac caacttcagc gccgagaccg ccgcccaggt gctgacccag gaggagcgcg
     3121 tcaatcagat ggaagtggag atcgaccacg atctgtccac catcatcgcg cggcgccagc
     3181 ccacggcgcg cgacctgcgc ctgctgatgg cgatctccaa gaccaccgcc aacctcgagc
     3241 gcgtgggcga cgaggcggcg cgcatcgcgc gcaccgtgca aaagcacgtc agcagcggcg
     3301 tatccggccg cctgaagctg ccggtgtccg acgtctcctt cgaggccgat ctggccaccg
     3361 cttcgctgcg caaggcgctg gatgccttcg cgcgcctgga cacggccaag gccctggaag
     3421 tgatcaagca ggacgaccag atcgacgagg aattcgacgg gatgatgcgc aagctcatca
     3481 cctacatgat ggaagacccg cgcacgatct cggccagcat cgatctcgtc ttcgtcgcca
     3541 aggcgatcga gcgcgtgggc gaccatgcga agaacctcgc cgagcagatc atctacatcg
     3601 tcaagggcac cgacgtgcgc cacaacccgg tcgagaccgt ggaatcgatg gtgcgctgag
     3661 cgaatagaga gtagagaggg aaaggggcat ccgatgagcc gaatactggt ggtggaagac
     3721 gaatcggcga tcgccgaact catctccatc aatctgcgcc atgcaggcta cgaggtgacg
     3781 gtagccatca cggccgatca ggcccagctg gaagtggacc gggtgctgcc cgacctcgtc
     3841 atcctcgatt ggatgctgcc cggccagtcc ggcctggcgc tggccaggcg ctggcgcggc
     3901 gaggcacgca ccaaggaact gcccatcatc atgctgaccg cgcgcgccga cgagggcgac
     3961 aaggtggcgg gcctggatgc gggcgccgac gactacctga ccaagccctt ctcgaccaac
     4021 gagctgaagg cgcgcatccg cgccgtgctg cgccgcaagg cgcccgaggc gctcgattcg
     4081 gccgtcgagc tgggcggcct gcgcctggac ccggcgacgc gtcgcgtggc gcgcggggac
     4141 caggaagtca agattggccc gaccgagttc cggcttttgc atttcctcat gacccacccc
     4201 gagcgcgtgc acagccgcgc ccagctgctc gatcgcgtct ggggcgacca tgtcttcatc
     4261 gaggagcgca cggtggacgt gcacgtcaag cgcctgcgcg aggcgctgtt gccggccaat
     4321 tgctcgcaga tgatcgagac ggtgcgcggg gcgggctacc ggctgacgca gcagagccag
     4381 gcgctgtccg cctgatccgt cgcctgcaag cgctccgccg ccgccccacg aggattgctc
     4441 cgtgacttgg ttcttccccc gcatcgcgat gcgcgtcctg acgctgctgg ccggcggctt
     4501 gctgggtgat ctgctgggcg gggtcttcaa cgcagcgacg cccggcttgc tgctcggcgt
     4561 ggcgctggcg gtgagcgcgc tgttgatctt cgacatgctg cgcggctatc gcctgatcaa
     4621 ctggctgcgc ggcccccaag agcagccggc gccgcgcgac accggcttct ggggcgagat
     4681 ggcctatcgc gtcgagcggg ccatccgcgt gcgcgagctc aacgccgagc aggagcagac
     4741 gcggctcgcg cagttcctct ccgccatcga agcctcgccc aacggcgtgg tgctgctgga
     4801 cgtcaatgac cagatcgact ggtgcaactc ggtcgcggcc gaccacttcg ggctggatcc
     4861 gctgcgcgac cgccgccagc gcatcacgaa cctggtgcgc gcgccggcct tcgtggtcta
     4921 tctgcagctg ggccgcttcg acgacccggt taccttcccg gttgcgcgtg ggcggggcac
     4981 gctgtcggtg ctggtgcgcc gctacggcga gggcaagaag ctcatcctgt cgcaggacat
     5041 caccgagcgc gagcgcaatg acgcgatgcg gcgcgacttc gtggccaatg tctcgcacga
     5101 gatccgcacc ccgctgaccg tgctggccgg cttcatcgag accatgagca gcctgccgct
     5161 caccgaggtc gagcgccgcc gcgttctcac cttgatgacg cagcagaccc agcgcatgca
     5221 gaccctggtc agcgatctgc tgctgctggc ccagctcgaa ggcagcccgc ggccgagcgc
     5281 ggaccgctgg gtggctgtgt ccaagctgct gtcccatgtg gagagcgacg cgaccacgct
     5341 gtcgcaaggg cggcacaagc tgagcttcgg tggtgcgccg ggccaggatc tctccgccgc
     5401 gcagatcgcc ggctccgaaa gcgagctgct gagcgccctg gccaatctgg tcaacaacgc
     5461 ggtgcgctac acgcccgagg gcggccagat cgacgtggtc tggcggctgc gcgacgacgg
     5521 ctcgggcgag ttcgccgtca tcgataccgg tatcggcatc gacaaggagc acctgccgcg
     5581 cctggccgaa cgtttctacc gcgtcgatgg cagccgctcg cgcgataccg gcggcacggg
     5641 tcttggcctg tccatcgtca agcatgtcgc ccagcgccat ggcggcgagc tgagcatcac
     5701 gagcgaaccc ggcaagggct ccaccttccg catcgtcttc ccggcttcgc gcgtgcgcta
     5761 catcgagccg gctaccgatc cgcggcggga aagcgccgaa acggtcgccg gcgctcgcta
     5821 ggcgggtcgg cgcggcgcgc cgtttcctga gcgaccagtc ccgcagggcg cttgagcctc
     5881 tttcgaagca ccagcttttc agggcatctg gggcgatgtg gtagaacccc gccccaagtc
     5941 gtatttcgga gttccttcca tgagtcagca tcccgtccag ctcgtccgcc gcccgccgcc
     6001 cgtcgcatcc acagcgagca gcggcacgca gcgctggtcg gtcgacgccg tcgctgcgct
     6061 cttcgagctg cccttcaacg acctgctgtg gcgggcgcag caaacgcacc gcgagcattt
     6121 cgacgccaac gaggtgcagc gctccacgct gctgtcgatc aagaccggcg gctgcccgga
     6181 ggattgcggc tattgcccgc agtcggtgca ctacgacacc ggcgtcgagg ccggcaagct
     6241 gatggatgtg caagccgtcg tggaggctgc ccaggccgcc ttgaatgccg gcgccacccg
     6301 cttctgcatg ggcgccgcct ggcgcgagcc caaggaccgc gacatcgaga aggtggccga
     6361 gctggttcgc gaggtgaagg ccctgggcct ggagacctgc tgcacgctgg gcatgctgag
     6421 cgctccccag gcgcaagccc tcaaggtcgc cggcctggac tactacaacc acaacctcga
     6481 caccgcgccc gaggcctatg gccgcatcat ctcgacgcgc gactaccagg agcggctcga
     6541 caccctggcc catgtgcgcg aggccggcat gtcggtctgc tgcggcggca tcgtcggcat
     6601 gggcgaatcc cgccgcgagc gcgcaggcct gatcgcccag cttgccaacc ttgagcccta
     6661 tcccgaatcg gtgccgatca acgagctggt gcaggtggaa ggcacgccgc tgcacggcac
     6721 cgacaagctc gacccgctgg aattcgtgcg caccatcgcg gtggcgcgca tcaccatgcc
     6781 caaggcacgc gtgcgcttgt cggccgggcg ccagcaaatg agcgaggcgg tgcaggcgct
     6841 gtgcttcctg gccggcgcca actccatctt ctacggcgag aagctgctga ccaccgggaa
     6901 cccggatgtg gcgcgggatg aggccttgtt cgagaagctg ggtttgcatg cagcctgact
     6961 ggcgccgtca cgcatgaccg ccgtcatcat ccgccacctc gccaagcacc aggaggtttt
     7021 gcctgtcctg caggcttggt tcgagtcgga gtggccggct cactatgggc ctggaggccg
     7081 cggtgacgcg cggcaggatt tgctcgccta cagcaacgcg ggcagcgtcc ccatcggctt
     7141 cgtcgcgttt cgcggcggcg ttccctgcgg cttcgcggcg ctcaagcgcg aggcctttgc
     7201 gacccacccg cagctgtcgc cctgggctgg tgccgcctat gtcgagccgg cgctgcgccg
     7261 gcagggcata ggccgagcct tggtggcctc gctcgaggcc gaggcgaggc gccttggcta
     7321 cccgcgcatc tatggggcga cttcgacggc gggctcgttg ttggagcggt gccggtggag
     7381 cttgctggaa agcgtggtgc acgatgggga gcaggtggct gtctatgaaa aggcgctctg
     7441 actcttgagc gcgcgcaaag tcgccctcat cggcgccggc cccgccggcc tgatggccgc
     7501 agaagtgctg gcccagcacg aaggtatcca ggtcgacctc tacgacgcga tgccttcatc
     7561 cggccgcaaa ttcctgctcg ccggcaaggg cggcctgaac ctcacgcatt ccgaaccgat
     7621 cgagcccttc ctcggccgct acgctgcgcg gcgcgctcag gtcgagcctt ggctgcgcga
     7681 attcggtcct gaggacttgc gcgcctgggc gcaggggctg ggcgtcgcca ccttcgtcgg
     7741 cacctcgggc cgcgtgttcc cgaccgacat gaaggccgcg ccgctgctgc gcgcctggct
     7801 gcaccgcctg cgcgaatcgg gcgtgcgcct gcacatgcgg catcgctggc tgggctgggc
     7861 cgaggatggc cgtgcactgc gcttcgcgac gccgcagggc gagcaggcga tcgaggccga
     7921 cgccgtgctg ctggcgctgg gcggcgcgag ctgggcgcgg ctgggctcgg acggcgcctg
     7981 ggtgccctgg ctgcgcgacc gcggcgtcga ggtggcaacg cttgcgccgg ccaattgcgg
     8041 cttcgacgtc gggcagggcg cccaggctgt tgaaggccgg ccggcacagg gctggagcga
     8101 gcattttcgc agccgccacg ccggcgagcc gctgaagggc atgggcctgc gttttgtcaa
     8161 cgcagacggc ggggcgcatt tcgagcgcgc gggcgagttc atcatcaccg aaggcggcgt
     8221 cgaaggcagc ctgatctatg ccgcctcggc gctgctgcgc gacgagatcg ccgcgcgtgg
     8281 cgaagcggtg atccagctcg atctattgcc cgctcgcgat gccgcctggg tgcgaaccga
     8341 ggtggcgcat ccgcgcggct cacgctcgat gtccagccac ttgcaaagcc gtcttgggct
     8401 gcacggcgcc aaggccgcgc tgctgcgcga gctgttgccg gccgaggcgt tccagcggcc
     8461 cggggcgctg gccgccgcca tcaaggccct gccgctgcgc ctggtggcgc cgcgcccggt
     8521 cgacgaagcg atcagcagcg cgggcggcgt gcgtttcgaa gcgctggacg ccgagctgat
     8581 gctgaaggcg ctgccgggcg tgtactgcgc cggtgagatg ctggactggg aggcgcccac
     8641 cggcggctat ctgctcaccg cctgcttcgc cagcgggcgc gcggcggcgc gtggtgtgct
     8701 gcgccatctc gggcgcgact gagcgttccc tactttgcgc cgcggtcgtt gatgtcggcc
     8761 cggcccgaga tgctgcgctt cacgacgggc gggttgcccc agtagtccac ggtgccgatg
     8821 ccggcgatcg agacgtcgag ctcgcgcgag acccagacgc cgacattgcc cacgccgctg
     8881 atcgccacct tggcctgctc gctcatcagc ttggcgccct ggaaattgct cttgccagcg
     8941 atacgcaggc tcaagctctt gacttcgccc gccatctggc cgtcgccggc gcccgagacc
     9001 tggaacacga gctgctcggc cttgagtcgg tcgaagcgcg ccgtgccggc acctgagatg
     9061 tcgatggtca ggagctcgac attcaccggc tgcggtgcga cgaaatccgc ggagccggag
     9121 attgccaccc gtttgaggtt gcgtgccgtc acgttgatct gcagccgccg cgcgctccag
     9181 aacttccagg cgccgctctg gttgatgctg agcgtgccgt tgcgcacctc cacgtccacc
     9241 gtgtcctgca ccccctggtc gccctcgatg agcacctggt cgctggcgcc ctgggtgaag
     9301 cgcaccacgg cggagccgct gatttcgatg gcatcgaagg aggggcccgg cacgaatttg
     9361 cggccctccg gactctgcgc gcgggccgcg ccaagaccac aagccagtgc gagcgcgaag
     9421 aggaagaaga aaaggcggcg tttcatagga ccaatgacct caggtgcttg gagacttcgc
     9481 ggctggccgt gacgctggtg ccgtcgcgca gcttgacg
//
LOCUS       DASUCY010000377         2182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1084446, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000377 DASUCY010000000
VERSION     DASUCY010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084446"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VFV25_07625"
     CDS             <1..343
                     /locus_tag="VFV25_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393230.1"
                     /translation="KLVTELSKVRDDVTRRGQKPPHTLYVLDEPTVGLHMADVEKLIR
                     VLHRLVDGGHSVVVIEHDLDVIAEADWVIDLGPEGGAAGGEVVVAGAPEAVVAAGSHT
                     GRALRPVLARR"
     gene            complement(392..>2182)
                     /locus_tag="VFV25_07630"
     CDS             complement(392..>2182)
                     /locus_tag="VFV25_07630"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY1393231.1"
                     /translation="AANPQPNATTAAPAGFGPDAQRTTCAALLAEADAAQRSGDHRGG
                     VQKAEAALAACLPGDDVLRAKALCLLAMHRWRLGQFEQAITATRDALPLLEREDRETA
                     ARCEALCLMAVSYSELGLQEEALKQALMAFDLARERGDRLWMAWALNRIGICYERLGD
                     PAQGERFLLQALDESLSLNDADIKFTSLNNLMAAMVGAYHLHRRRRDAEGEANAHSAL
                     QRARLYGHEAMTLARHVGDRYREAVTIGNLGEVLGLSGESEAAFKLLDKALVIAHTEG
                     YRALELRTLHSIGEVSGLCGAHQRAVDLLEPALSKISMGEHETTRMRMHSALHRSYKA
                     LQRYREALEHHEAFYALESRRAALQHQAQARLMVNRIEVEQALMQGERSRLEAERERL
                     KTQQLEAEKRKLEASTEELHRHALEDQLTGLANRRRVDHDLPELFGRARESGKPLSVA
                     VADLDNFKTINDEHGHLLGDEVLRAVAQIFRANTRGSDVLARFGGEEFLLLFVDTPLD
                     IAQEVCERLRLAIETYPWQRLAPDLGVTISLGLCDATPDPDPTRMLARADGALYYAKR
                     TGRNRVSVDDPPGSSSGFGGLGPPESQHKH"
BASE COUNT          312 a          732 c          790 g          348 t
ORIGIN      
        1 caagctggtg acggagctct cgaaggttcg cgacgacgtg actcgaagag gccagaagcc
       61 gccgcacacg ctctatgtgc tggacgaacc caccgtaggg ctgcacatgg ccgatgtcga
      121 gaagctgatc cgcgtgctgc atcgattggt cgacggcggc cacagcgtgg tggtgatcga
      181 gcacgacctc gacgtgatcg ccgaggccga ttgggtcatc gacctcggcc ccgagggcgg
      241 cgcggcgggc ggcgaggtag tggtggcggg tgcgcccgag gcggtggtcg cggcgggcag
      301 tcacaccggc cgggctctca ggccggtgct ggcgcggcga tgaggcaggc gcctgcggta
      361 tgcccgcgga caaggccggg gtactgcgag ttcaatgctt gtgctgagac tccggcgggc
      421 ccagccctcc gaatccggag gaagagcccg gcgggtcgtc cacgctgacg cggttgcggc
      481 ccgtgcgctt cgcgtaatac aacgcgccgt cggcacgggc cagcatgcgc gtggggtcgg
      541 ggtcgggcgt ggcatcgcac aggcccaggc tgatggtgac gcccagatcc ggcgcgaggc
      601 gctgccaggg ataggtctcg atcgccaggc gcaggcgctc gcagacctcc tgcgcgatat
      661 ccagcggggt gtcgacgaag agcagcagga actcctcgcc gccgaagcgg gccagcacat
      721 cggagccgcg cgtgttggcg cggaagatct gggccaccgc gcgcagcacc tcgtcgccca
      781 gcagatggcc atgctcgtcg ttgatggtct tgaagttgtc caggtcggcc accgcgacgc
      841 tcaggggctt gcccgactcg cgggcgcggc cgaaaagctc gggcaggtcg tggtcgacgc
      901 ggcgacggtt ggccaggccg gtgagctgat cttccagcgc atggcggtgc agttcctccg
      961 tgctcgcctc cagcttgcgc ttctcggcct cgagctgctg ggtcttcagg cgctcgcgct
     1021 cggcctccag ccgcgagcgc tcaccctgca tcagcgcctg ctcgacttcg atgcgattga
     1081 ccatcagcct ggcctgggcc tgatgctgca aggcggcgcg ccggctctcc agcgcgtaga
     1141 aggcctcgtg atgctccagc gcctcgcggt agcgctgcaa ggccttgtag ctgcggtgca
     1201 gggccgaatg catgcgcatg cgcgtggtct catgctcgcc catcgagatc ttgctcagcg
     1261 ccggctccag caggtctact gcgcgctggt gcgcgccgca gaggccgctg acctcgccga
     1321 tgctgtgcag ggtgcgcagc tcgagcgcgc gatagccctc ggtgtgcgcg atgaccagcg
     1381 ccttgtcgag cagcttgaag gcggcctcgc tctcgcccga aagccccagc acctcgccca
     1441 ggttgccgat cgtcaccgcc tcgcgatagc ggtcgccgac gtgacgcgcc agcgtcatcg
     1501 cctcgtggcc gtagagccgg gcacgctgca aggcgctgtg tgcattggcc tcgccttcgg
     1561 cgtcgcggcg gcgccggtgc aggtggtagg cccccaccat cgcggccatc agattgttga
     1621 gcgaggtgaa cttgatgtcc gcatcgttca ggctcaggga ttcgtccagg gcctgcagca
     1681 ggaagcgctc gccctgggcc gggtccccca ggcgctcgta gcagatgccg atgcgattga
     1741 gcgcccaggc catccacagc cggtcgccgc gctcgcgtgc gaggtcgaag gccatcaggg
     1801 cctgcttcag cgcctcttcc tgcaggccca gctcgctgta ggagacggcc atcaggcaca
     1861 gggcctcgca gcgcgcggcg gtctcgcggt cttcgcgttc cagcagcggc agggcgtcgc
     1921 gcgtggcggt gatggcttgc tcgaactgcc cgagccgcca gcgatgcatg gccagcaggc
     1981 acaaggcctt ggcccgcagg acgtcatcgc caggtagaca agctgccaat gccgcctcgg
     2041 ccttctgcac gccgccgcgg tgatcgccgc tgcgctgggc cgcatcggcc tcggcaagca
     2101 gagccgcgca ggttgtccgc tgggcgtcag ggccaaaccc ggccggcgcc gcagtggtgg
     2161 cgttgggctg agggttggca gc
//
LOCUS       DASUCY010000378         2154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_426019, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000378 DASUCY010000000
VERSION     DASUCY010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426019"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..1297
                     /gene="senA"
                     /locus_tag="VFV25_07635"
     CDS             104..1297
                     /gene="senA"
                     /locus_tag="VFV25_07635"
                     /inference="COORDINATES: protein motif:HMM:NF041186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1903257 - selenoneine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenoneine synthase SenA"
                     /protein_id="HEY1393232.1"
                     /translation="MADVRRLSGAELAAALRDSRAVTLSRVEDLSDAQWAVPQRDGVN
                     PVAWELAHLAWFGEFWILRGPHQLGDDGYVHAQRPVRIAGPDAHLDSARLPHARRWTT
                     ELPSRSQVAGMLEAQLAACLQALPEGDDDAALYFHRLALFHEDMHGEAFSWMRAALGY
                     PAPVGASLPHVPEARSLLVPGGEVRIGWPPGRRGFAFDNEGAGASLVLRDYEIDDAPV
                     SVGQFLRFVEAGGYEQPDYWPGEAGRWRASSPWPHPARWRRTAGAWELRWFDRWLPLD
                     PEQPVIHVNAWESEAYCSWARRRLPSAAEWEHAALMSQGSQSGMRWGHNVWEWTASTF
                     APYAGFAAGPYRDYSKPWFGDHRELRGGAFATHARMHDARYRNFFQPQRCDIFAGFRT
                     AAPQR"
     gene            1309..>2154
                     /locus_tag="VFV25_07640"
     CDS             1309..>2154
                     /locus_tag="VFV25_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY1393233.1"
                     /translation="MHHPSRLLGRLALPVLLLLGCAAAGAQALTTLRVSAIPDEAPTE
                     LQRKFKPLGEYLEKETGLKVQFTPVTDYAAVVEGLANDKIDMAWLGGFTFVQAKLRTH
                     GGVTPIVQRAEDEKFTSKFIVPLASTANSLADLKGKNFVFGSPSSTSGHLMPRYFLMK
                     AGIDADKDFKAVAFSGAHDATVAYVASGRAEAGVLNASVWDKLVEAHNPNAGKVRVLA
                     TTPPYFDYNWTVRPGLDAALTKKLTAAFLKLDPANPEHKEIMALQRASKFIPTQASNY
                     DGIEAA"
BASE COUNT          334 a          735 c          723 g          362 t
ORIGIN      
        1 gccgttgttg gcctctggcc gagccggcgt gacgatgcag atgcggggtt tcttgctcat
       61 gtttgcgtcg aggctggcat cttgacgtgc ccaggagcgc ttcgtggctg atgtgcgccg
      121 actgagcgga gccgagctcg cagcggcgct gcgcgacagc cgcgccgtca cgctttcgcg
      181 ggtcgaagac ctgagcgatg cgcaatgggc cgtgccgcag agggacggcg tgaacccggt
      241 ggcctgggag ctggcccatc tggcctggtt cggcgagttc tggatactgc gcggcccgca
      301 tcaactgggc gacgacggct atgtgcatgc gcagcgcccg gtgcgcatcg cggggcccga
      361 tgctcacctc gactcggcgc gcctgccgca cgcgcggcgc tggaccacag agctgccgtc
      421 gcgctcgcaa gtcgccggga tgctggaggc ccagctcgca gcctgcctgc aggccttgcc
      481 cgagggggac gatgacgcgg ccctgtactt tcaccgcctc gcgctcttcc atgaagacat
      541 gcacggcgag gccttctcat ggatgcgagc agcgctcggc tatccagcgc ctgtaggcgc
      601 gagcctgcct catgtgccgg aagcacgctc gctgctcgtt ccgggcggcg aggttcgcat
      661 cggctggccg ccggggcgtc gcggcttcgc cttcgacaac gaaggcgccg gcgccagcct
      721 cgtgctgcgg gactacgaga tcgacgatgc gccggtgagc gtcgggcagt tcctgcgctt
      781 cgtcgaggcc ggcggctatg agcagcccga ctactggccg ggcgaggcag gacgctggcg
      841 cgcatccagc ccttggcccc atcccgcgcg ctggcgccgc acggccggtg catgggaatt
      901 gcgctggttc gaccgctggc taccgctcga tcccgagcag ccggtgatcc atgtcaatgc
      961 ctgggaatcc gaggcttatt gcagctgggc ccggcggcgc ctgccgagcg cggccgaatg
     1021 ggagcatgcc gcgctgatgt cgcagggctc acagtcgggc atgcgctggg gccacaacgt
     1081 ctgggaatgg acggcaagca ccttcgcgcc ttatgccggc ttcgccgctg gcccctaccg
     1141 cgactactcc aagccctggt tcggcgatca tcgcgaactg cgtggcggcg ccttcgccac
     1201 tcatgcacgc atgcacgatg cgcgctatcg caatttcttc cagccgcagc gctgcgacat
     1261 cttcgctggc tttcgcacgg cggctccgca acgataaaag gaggtctcat gcatcacccc
     1321 tcacgactgc tcggccggct ggccctgcct gttctgctgc tgctcggctg cgccgcggcc
     1381 ggcgcccagg cgctgacgac gctgcgcgtc tcggccatcc cggatgaggc gcccaccgag
     1441 ctgcagcgca agttcaagcc gctgggcgag tatctggaga aggaaacggg gctgaaggtg
     1501 cagttcacgc cggtgaccga ctacgctgcg gtcgtcgaag gcctggccaa cgacaagatc
     1561 gatatggcct ggctgggcgg cttcaccttc gtgcaggcca agctgcgcac gcatggcggt
     1621 gtcacgccca tcgtgcagcg cgcagaggac gagaagttca ccagcaagtt catcgtgccc
     1681 cttgccagca cggccaatag ccttgcggac ctgaagggca agaacttcgt cttcggctcg
     1741 ccttcgtcga cctccggcca tctgatgccg cgctacttcc tgatgaaggc cggcatcgat
     1801 gcggacaagg acttcaaggc cgtggccttc tccggggcgc acgacgccac ggtggcctat
     1861 gtcgcctcgg gccgcgccga ggcgggcgtg ctcaatgcct cggtgtggga caagctggtc
     1921 gaagcccaca accccaacgc cggcaaggtg cgcgtgctgg ccacgacgcc gccctacttt
     1981 gactacaact ggaccgtgcg gcccggcctg gatgcagcgt tgaccaagaa gctcacggcg
     2041 gctttcctca agctcgatcc ggccaatccc gagcacaagg aaatcatggc gctgcagcgc
     2101 gcatccaagt tcatcccgac ccaggcctcg aactacgacg gcatcgaggc ggcc
//
LOCUS       DASUCY010000379         2176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_249319, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000379 DASUCY010000000
VERSION     DASUCY010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249319"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VFV25_07645"
     CDS             <1..495
                     /locus_tag="VFV25_07645"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561819.1"
                     /note="catalyzes the formation of porphobilinogen from
                     5-aminolevulinate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEY1393234.1"
                     /translation="APSDMMDGRIGAIRQALEAKGRIHTRIMAYSAKYASAFYGPFRD
                     AVGSKFSLGKADKKTYQMDPGNSDEALREVALDIAEGADMVMVKPGMPYLDIVRRVKD
                     EFRVPTFAYQVSGEYAMLKAAAANGWLDHDAAMMEALLAFKRAGADGVLSYFALEAAR
                     LLKA"
     gene            complement(527..1192)
                     /locus_tag="VFV25_07650"
     CDS             complement(527..1192)
                     /locus_tag="VFV25_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEY1393235.1"
                     /translation="MITVHHLNNSRSQRVLWLLEELELSYEIKHYQRDPQTMLAPPEL
                     RRIHPLGKSPVITDGELTLAESGAILEYLVARYGAGRFRPAPGTPEALRYVYWMHFAE
                     GTAMPPLLMKLVFDRIEHKSPLLVRPIAGAIARKAKAMIVTPSLQRHLDFMEAELGNR
                     PWFAGDEFSAADIQMSFPVEASAVRAGLDASRPRLLDFLQRIHARPAYARALQRGGPF
                     ALA"
     gene            1319..1858
                     /locus_tag="VFV25_07655"
     CDS             1319..1858
                     /locus_tag="VFV25_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HEY1393236.1"
                     /translation="MSDENQTPTPVFTHVVANRRALRTIAVFEAWKGLIAVAAVLGLL
                     SPLRHDLHHIAIALIGHIGLDPGDRYPAILLHYLDVLRDANLRSLMLIAAGYVLLRWC
                     EAYGLWHERAWGEWLGALSGALYVPFELRHLLHRASWIGALVLISNIAIVAYLAWRLW
                     HRRRATHPHGGLEAPPQEN"
     gene            complement(1865..>2176)
                     /locus_tag="VFV25_07660"
     CDS             complement(1865..>2176)
                     /locus_tag="VFV25_07660"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase light chain EutC"
                     /protein_id="HEY1393237.1"
                     /translation="EIGAALHARAVAMLLGERPGLSSPDSLGAYLTWAPRVGRSDAER
                     NCVSNIRPEGLAYADAARKLAWLCTAAARLGASGVPLKDESDAPALPDCPQAHSGADA
                     A"
BASE COUNT          334 a          737 c          767 g          338 t
ORIGIN      
        1 gcgccctccg acatgatgga cggccggatc ggggcgattc gccaggcgct cgaggccaag
       61 ggccggatcc acacccgcat catggcctac agcgcgaagt acgcctcggc tttctacgga
      121 cccttccgcg acgcggtggg ctccaagttc agcctgggca aggccgacaa gaagacctac
      181 cagatggacc ccggcaacag cgacgaggct ttgcgcgaag tcgcgctgga catcgccgag
      241 ggggccgaca tggtgatggt caagcccggc atgccttacc tcgacatcgt gcgccgcgtg
      301 aaggacgagt tccgcgtgcc gaccttcgcc taccaggtga gtggcgaata cgcgatgctc
      361 aaggctgccg ccgccaacgg ctggctggac cacgacgcgg cgatgatgga agccctgctg
      421 gccttcaagc gcgccggcgc cgacggcgtg ctgagctatt tcgcgctcga ggccgcgcgg
      481 ctgctcaagg cctgaggccc gacactgaca ccgcggctgg cgaccctcaa gccaatgcga
      541 acggaccgcc gcgctgcaag gcccttgcat aggccggtcg cgcgtggata cgctgcagga
      601 aatcgagcag ccggggccgg ctcgcatcga gcccggcgcg caccgccgag gcctccacag
      661 ggaagctcat ctggatatcg gccgcgctga actcgtcgcc tgcgaaccag gggcggttcc
      721 ctagctcggc ctccatgaag tcgaggtggc gctgcaagct gggcgtcacg atcatggcct
      781 tggccttgcg cgcaatggcg ccggcgatcg ggcgcacgag cagcggcgac ttgtgctcga
      841 tgcggtcgaa caccagcttc atcagcagcg gcggcatcgc cgtgccttcg gcgaaatgca
      901 tccagtagac gtagcgcagc gcctccggcg tgccgggtgc gggcctgaag cggcctgcgc
      961 cgtagcgcgc gaccaggtat tcgaggatcg cgcccgactc ggccagcgtc agctcgccgt
     1021 cggtgatgac cggggacttg cccagcgggt gtatgcgacg cagctcgggc ggcgccagca
     1081 tggtctgcgg gtcgcgctgg tagtgcttga tctcgtagga cagctcgagc tcctcgagca
     1141 gccacagcac gcgttgcgag cgcgagttgt tcaggtggtg gacggtgatc atggccgggc
     1201 cggtgttgtg gaagagctga gcatgccagt tcagggccgg cgggtcctga ggcgagaatg
     1261 acgggagccg gcaggccgat ggggtctgcc gacccccctg ttgtatcagc cgacccagat
     1321 gtccgacgag aaccagactc ccacgccggt cttcacccat gtcgtcgcca atcgccgcgc
     1381 gctgcgcacg atcgcggtct tcgaggcctg gaagggcctc atcgcggtgg cggccgtcct
     1441 ggggctgctc agcccgctgc gccacgacct gcaccacatc gcgatcgcgc tgatcggcca
     1501 catcggcctc gatccgggtg accgctaccc ggccatcctg ctgcactacc tggatgtgct
     1561 gcgtgacgcc aacctgcgct cgctgatgct gatcgccgcg ggctatgtgc tgctgcgctg
     1621 gtgcgaggcc tatggcctgt ggcacgagcg ggcctggggc gagtggctcg gggccttgtc
     1681 cggcgcgctg tacgtgccct tcgagctgcg gcatctgctg caccgggcga gctggatcgg
     1741 cgcgctggtg ctgatcagca atatcgcgat cgtggcctac ctcgcctggc ggctgtggca
     1801 taggcgtcgg gcgacccacc cccacggcgg cctcgaagca cctcctcaag agaactgagc
     1861 cgggtcacgc cgcatcggct ccgctatgcg cttgaggaca atccggcagc gcgggtgcat
     1921 cgctctcgtc cttgagcggc acgccgctgg cgccgagccg cgccgcggcc gtgcagagcc
     1981 aggccagctt gcgcgccgcg tcggcatagg ccaggccctc agggcggatg ttggacacgc
     2041 agttgcgctc ggcgtcgctg cgccccacgc gcggggccca ggtgagatag gcgcccaggc
     2101 tgtcgggcga ggacaggccg gggcgttcgc ccagcagcat cgcgaccgcg cgggcatgca
     2161 gcgcggcgcc gatttc
//
LOCUS       DASUCY010000380        10271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1437882, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000380 DASUCY010000000
VERSION     DASUCY010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 10271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10271
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1437882"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VFV25_07665"
     CDS             <1..522
                     /locus_tag="VFV25_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HEY1393238.1"
                     /translation="MGFIVFMGVAFGFFYAIEKTKNSSLGVVLLLAFTFFMGLMLSRL
                     IGTVLGFRNGAQLIGMAFGGTAIIFFGMAALASAVKRDLSGLGKWLMIGALLAIVAGV
                     ANIWLQMPALFLTISVLVMAIFSAFIVYDLKRVIDGGETNYITATLAIYLDVFNVFQS
                     LLSILGVLGGERD"
     gene            complement(598..2031)
                     /gene="dnaB"
                     /locus_tag="VFV25_07670"
     CDS             complement(598..2031)
                     /gene="dnaB"
                     /locus_tag="VFV25_07670"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEY1393239.1"
                     /translation="MSAILSGADFPGSSPRGGDNEVARLRIPPHSIEAEQSVLGGLLL
                     DNSAWDRAGDLLGDSDFYRYEHRLIYAAIGGLINANKPADVITVFEQLQSLGKAEEIG
                     GLAYLNSLAQSVPSAANLRRYAEIVRERAILRKLVSASDEIATSAFNPQGRPVPQILD
                     EAEGMIFKIGEEGSRTKQGFHSMESLVVDLLDRVQELHDNGAEEVTGVRTGFYDLDRM
                     TAGLQPGDLIVLAARPSMGKTAFALNIAEHVAVQEGLPSVVFSMEMGASQLALRMVGA
                     LGRIDQQRLRTGALHDDEWLRLTEAVEKLRNVSLFIDETPGLTPSELRARARRQARQC
                     GKLGLIVVDYLQLMSGSGGGSEENRATVLGEISRGLKALAKELQCPVIALSQLNRSVE
                     TRTDKRPMMSDLRESGAIEQDADVIMFIYRDDYYNKDASKEPGVAEIIIGKQRNGPVG
                     TIKLAFLKPQTKFENLAPGSVGAGSDY"
     gene            complement(2194..2646)
                     /gene="rplI"
                     /locus_tag="VFV25_07675"
     CDS             complement(2194..2646)
                     /gene="rplI"
                     /locus_tag="VFV25_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEY1393240.1"
                     /translation="MQVILLEKVANLGNLGDVVKVKDGYARNFLIPTGSARRATEAAR
                     KEFEAKRAELEKAQADKLGAAQAEGEKLNGKNVSVTQKAGVDGRLFGSVTNFDIAEAL
                     KKQGFSVIKAQVRLPNGPLKVVGEHAVSVALHTDVVVDVTVTVVGETD"
     gene            complement(2662..2952)
                     /gene="rpsR"
                     /locus_tag="VFV25_07680"
     CDS             complement(2662..2952)
                     /gene="rpsR"
                     /locus_tag="VFV25_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEY1393241.1"
                     /translation="MPPPRGKGRFNKDRKTKRNQQSLLFRRKRFCRFTVAGVKEIDYK
                     DIDTLRDFIAENGKIIPARLTGTRAIFQRQLTTCIKRARFLALLPYSDQHRV"
     gene            complement(2962..3267)
                     /gene="priB"
                     /locus_tag="VFV25_07685"
     CDS             complement(2962..3267)
                     /gene="priB"
                     /locus_tag="VFV25_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HEY1393242.1"
                     /translation="MNRLVLRAQVVERGAMRYTPAGLPALDLSLKHESEAMEAGQPRK
                     VSMEMRAVGIGAIAERLAKLAVGKTAGFAGFLGSMRNGRGVLFHVTELDELDSDLES"
     gene            complement(3317..3685)
                     /gene="rpsF"
                     /locus_tag="VFV25_07690"
     CDS             complement(3317..3685)
                     /gene="rpsF"
                     /locus_tag="VFV25_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEY1393243.1"
                     /translation="MRHYEIVLLIHPDQSEQVPAMLERYKGVVTAAGGKLHRVEDWGR
                     RQLAYMIDKLAKAHYLCLNIECGKETLAELETGFRFNDAVLRHLTVQKSKAETAPSLM
                     MKQVEREDARKSSQQEFNAA"
     gene            complement(3860..5947)
                     /locus_tag="VFV25_07695"
     CDS             complement(3860..5947)
                     /locus_tag="VFV25_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEY1393244.1"
                     /translation="MTQVTRSFAHKLHRRYGIFTVGVVIFIVGLGVLERLGWSRGWIG
                     ASFLAATVGIYAVIGLLSRTTDESEYYVAGRRVPAIYNGMATAADWMSAASFIGTAGV
                     LYLKGFGGLAYILGWTGGYCLVALLLAPYLRRFGQYTIPDFLGARYGGVLPRLVGVAC
                     AVLVSFIYVVVQIYGVGLITSHLTGFTFEIGIFVGLGGVLVCSFLGGMRAVTWTQVAQ
                     YIILIVAYLVPVVWISAKQTGNPVAVVAYGEQLAKVGERETALQADPRELAVSADLHQ
                     RAEGLRRKLADVPAAMQRDAQALTAQVARMKADDAPLARIQQAERRLMNLPRSQEAAA
                     AAYRRELDSAVLQAQPLAGLAPQTRPYAAVGEGDAQDQQAFKRERRNFLALVFCLMVG
                     TAAMPHVLTRYYTTPSVGEARRSVAWSLFFISLLYIAAPALAVMVKYEVFSHVVGLPY
                     DQLPDWVRRWSKLDPSLVSVEDVNGDGILQLGELHLGGDVIVLAAPEIAGLPLVVTYL
                     VAAGGLAAALSTADGLLLTISNALSHDFYHRVLNPKASAIRRVMLSKVMVLVVALAAA
                     YVASLHITDILQFVSAAFSLAAGAFFPALILGIFWRRANRAGATAGMLAGLGVCVYYM
                     ATNMPLLRGILGITRPLAECSWWGVEPIAAGVFAVPVGFMVLAVVSLLLPGSGQGEAG
                     LPDGVRYPRAGAG"
     gene            complement(5944..6267)
                     /locus_tag="VFV25_07700"
     CDS             complement(5944..6267)
                     /locus_tag="VFV25_07700"
                     /inference="COORDINATES: protein motif:HMM:TIGR03647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HEY1393245.1"
                     /translation="MPSVSLAVSAKQRAYWRAVLRVTGVLLLLWFLLSFVGPWFAASL
                     GAAHVGYFPLSFWMAAQGALLLFVAIVAVYALAMEHLDARYAAQDDDGGEGQGASPGP
                     AGREP"
     gene            complement(6349..8745)
                     /gene="ppsA"
                     /locus_tag="VFV25_07705"
     CDS             complement(6349..8745)
                     /gene="ppsA"
                     /locus_tag="VFV25_07705"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEY1393246.1"
                     /translation="MSSRFEATALVVPFENLRMTDVEVVGGKNASLGEMISQLAASGV
                     RVPGGFATTAHAFREFLKHGGLAERINARLAALNTDDVRALAEAGAQIRGWVEAQPFP
                     ASLEAAIRAEFAKLTAGNADASFAVRSSATAEDLPDASFAGQQETFLNVVGVEQVLHK
                     MKEVFASLYNDRAISYRVHKGFAHAEVALSAGVQRMVRSDLGAAGVMFTIDTESGFKD
                     VIFITSSYGLGETVVQGAVNPDEFYVHKPALKNGKLGIIRRNLGSKLIRMEFAGADEK
                     AASGKLVRTVDTPAELRNRYSLSDADVTELARYALVIEQHYGRPMDIEWGKDGGDGQL
                     YILQARPETVKSQAEGKVEHRYKLKGQGAVLAEGRAIGQKIGTGPVRLVHSIAEMERV
                     QPGDVLVTDMTDPNWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATETL
                     KDGALVTVACSEGDTGYIYDGLLETEVSEVQRGELPYCPVKIMMNVGNPQLAFDFAQM
                     PNSGVGLARLEFIINNNIGVHPKAILDYPNVDADLKKAVESVARGHASPRAFYVDKLA
                     EGIATIAAAFWPKPVIVRLSDFKSNEYRKLIGGSRYEPEEENPMLGFRGASRYISPEF
                     GEAFAMECEALKRVRTGMGLTNVEIMVPFVRTIKQAERVIAMLGEHGLKRGHDDLRII
                     MMCEVPSNAILAEQFLEHFDGMSIGSNDLTQLTLGLDRDSGLEQLAHDFDERDPAVKA
                     MISRAIAACRAVGKYVGICGQGPSDHPDFADWLASEGIVSISLNPDSVVETWQRLAKR
                     "
     gene            8965..9786
                     /locus_tag="VFV25_07710"
     CDS             8965..9786
                     /locus_tag="VFV25_07710"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HEY1393247.1"
                     /translation="MPNRTVFFVSDGTGITAETFGNSILNQFSGKPRHVRRPFIGTAD
                     KAYQVVREINETAEREGRKPIVFITLVNDEVLRIVVEQSRGLVLDMFKTFIEPLELEL
                     GVKSNHRVGRFSDVAKSREYDNRIEAINFSLAHDDGQSARNLEQADVILVGVSRSGKT
                     PTSLYLAMQHGVKAANYPLIPEDFERGKIPSSLAPHKKKCFGLTIDPERLSSIRNERR
                     PGSKYASLENCRYEVNEAEHMMKREGISWLSSTHKSIEEIATTILRDIRPDRLIY"
     gene            9970..>10271
                     /locus_tag="VFV25_07715"
     CDS             9970..>10271
                     /locus_tag="VFV25_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393248.1"
                     /translation="MSHTASPRNPAFDGLDFQLTDFDEARAALDELPPGINEVQRLKP
                     GFWDARRRKPMATDRALAGSTMDWVMGLTPGLRPRVLCERFPRIANALAACWLDEQR"
BASE COUNT         1768 a         3390 c         3375 g         1738 t
ORIGIN      
        1 atgggcttca tcgtcttcat gggcgtggcc ttcggcttct tctacgcgat cgagaagacc
       61 aagaactcga gcctgggcgt cgtgctgctg ctcgccttca ccttcttcat gggcctgatg
      121 ctgtcgcgcc tgatcggcac cgtgctcggc ttccgcaacg gcgcgcagct catcggcatg
      181 gccttcggcg gcacggccat catcttcttc ggcatggcag cactggccag cgcggtcaaa
      241 cgcgacctgt ccggtctcgg caaatggctg atgatcggcg cgctgctcgc gatcgtggca
      301 ggcgtggcca acatctggct gcagatgccg gcgctgttcc tgacgatctc cgtgctcgta
      361 atggcgatct tctcggcctt catcgtctac gacctcaagc gcgtgatcga cggcggcgag
      421 actaactaca tcaccgcgac gctggcgatc tatctggacg tcttcaacgt gttccagtcg
      481 ctgctgagca tcctcggcgt gctgggcggc gagcgcgatt gagcgcgctg tagcgcagca
      541 caagtcgaaa cggggcccgc gaggccccgt tttcattggt acgcgaacca gcgtgtttca
      601 atagtcgctg cccgccccta cgctgcccgg cgccaggttc tcaaacttgg tctggggctt
      661 caggaaggcc agcttgatcg tgccgaccgg gccattgcgc tgcttgccga tgatgatctc
      721 ggccacgccc ggctccttgg acgcatcctt gttgtagtag tcgtcgcggt agatgaacat
      781 gatgacgtca gcgtcctgct cgatggcgcc ggattcgcgc aggtcgctca tcatcggccg
      841 cttgtcggta cgggtctcga cgctgcggtt gagctgcgac aaggcgatca ccgggcattg
      901 cagttctttc gccagcgcct tcaggccgcg cgagatctcg cccagcacgg tggcgcggtt
      961 ttcctcgctg ccgccacccg agccgctcat cagctgcagg tagtcgacca cgatcaggcc
     1021 cagcttgccg cattgccgcg cctggcgtct ggcgcgggcg cgcaactcgc tgggcgtgag
     1081 accaggcgtc tcgtcgatga agaggctgac attgcgcagc ttctcgaccg cctcggtcag
     1141 gcgcagccat tcgtcgtcgt gcagtgcccc ggtgcgcagg cgctgctggt cgatgcgtcc
     1201 gagcgcgccc accatgcgca gcgcgagctg ggaggcaccc atttccatcg agaacacgac
     1261 cgagggcagg ccttcctgca ccgcgacatg ctcggcgatg ttgagcgcga aggcggtctt
     1321 gcccatcgag gggcgggcgg ccagcacgat cagatcgccg ggctgcaggc ctgccgtcat
     1381 gcggtcgagg tcgtagaagc cggtgcgcac gccggtcact tcctcggcgc cgttgtcgtg
     1441 cagctcctgc acgcggtcga gcaggtcgac gaccagcgac tccatcgaat ggaagccctg
     1501 cttggtgcgc gagccttctt cgccgatctt gaagatcatg ccctcggcct cgtcgaggat
     1561 ctgaggcacc ggccgcccct gggggttgaa ggccgaggtg gcgatctcgt cgctggccga
     1621 caccagtttg cgcaggatgg cgcgctcgcg cacgatctcg gcatagcgcc gcaggttggc
     1681 cgcgctcggc acgctttgcg ccagcgagtt cagataggcg agtccaccga tctcctcggc
     1741 cttgcccagc gactgcaact gctcgaacac cgtgatcaca tcggccggct tgttggcgtt
     1801 gatgaggcca ccgatggcgg catagatcaa ccggtgctcg tagcggtaga agtcgctgtc
     1861 gcccagcaga tcgccggcac ggtcccaggc gctgttgtcg agcagcaggc cgcccagcac
     1921 gctctgctcg gcttctatcg aatgcggcgg gatgcgcagg cgggcgactt cgttgtcgcc
     1981 gccgcgaggc gacgagcccg ggaaatcggc cccggagagg atggcggaca taagtgcaga
     2041 aggatggcgg ctagccgccg cgagggcaag tggacaaacc tgtgggtatg tggacaagcc
     2101 tgtgcatgac cggtgggcag gctggaaaaa cttgtgcgca aaagcaaaag gccggtcttg
     2161 cgaccggcct ttcaagtcac acgacgagcg tgatcagtcg gtctcgccca caacagtgac
     2221 ggtcacgtcg accaccacgt cggtgtgcag agccaccgac acggcatgct cgccgacgac
     2281 cttcagcgga ccgttgggca agcgcacctg ggccttgatg accgagaagc cctgcttctt
     2341 cagcgcctcg gcgatgtcga agttggtcac ggaaccgaac aggcggccat cgacgccagc
     2401 cttctgggtg acggagacgt tcttgccgtt gagcttctcg ccttcggcct gggccgcgcc
     2461 gagcttgtcg gcctgggcct tctcgagttc ggcgcgcttg gcctcgaact ccttgcgggc
     2521 cgcttccgtg gcgcggcggg cggagccggt ggggatcagg aagttgcggg cgtagccgtc
     2581 cttcaccttc accacgtcgc ccaggttgcc caggttggcg accttttcaa gcaggatcac
     2641 ttgcatgttc ttggctcctc tttagaccct gtgctggtcg ctgtagggca gcagagccag
     2701 gaagcgggcg cgcttgatgc acgtggtcag ctgacgctga aagatggcac gggtgccggt
     2761 caggcgggcc gggatgatct tgccgttctc ggcgatgaaa tcgcgcaggg tgtcgatgtc
     2821 cttgtagtcg atttccttga cgccggcgac ggtgaagcgg cagaagcgct tgcggcggaa
     2881 cagcagcgac tgctggttgc gcttggtctt gcggtccttg ttgaaccggc cttttccgcg
     2941 gggtgggggc atgttgagct cctaactttc caaatccgaa tctagttcat cgagttcagt
     3001 gacgtgaaac agcacgcctc ggccgttgcg catcgagccc aggaaaccgg cgaaacccgc
     3061 agtcttgccg actgccagct tcgcgagcct ctcggctatc gccccgatgc ccaccgcgcg
     3121 catctccatc gagaccttac ggggctggcc ggcttccatg gcctcggact cgtgcttcag
     3181 gctcaggtcc agggccggca ggccggcggg ggtgtagcgc atggcgcctc gttcgaccac
     3241 ctgagccctc aacaccaagc gattcatcgc gggctagggc ggcaggggaa gatcgagacg
     3301 gccgggcgcg gcgtcatcag gccgcgttga actcctgttg cgaggacttc cgggcgtctt
     3361 cgcgctcgac ctgcttcatc atcagcgacg gagcggtctc ggccttgctc ttctgcaccg
     3421 tcagatggcg cagcacggcg tcgttgaagc ggaagcccgt ttcgagctcg gccagggttt
     3481 ccttgccgca ctcgatgttc aggcacaggt aatgagcctt cgcgagcttg tcgatcatgt
     3541 aggccagctg gcggcggccc cagtcctcga cgcggtgcag cttgccgccg gcggcggtga
     3601 cgacaccctt gtagcgctcc agcatggctg gaacctgctc gctttgatcc ggatggatca
     3661 gcaaaacgat ttcataatga cgcatgcaaa ctccttgtgg attccaccgg gttcacggac
     3721 gaccgccgaa gagatcgccg tcaagccgac aggtaagccg cccctgagcg tcaactgtga
     3781 gggtgcggca aggcttggaa aagcctgaaa ttgtagcacg gccggctcca cccgggagac
     3841 ggcgagctcc tggctgcact caacctgcgc ctgcacgcgg gtagcgcacg ccatccggca
     3901 agcccgcctc gccctggccg ctgcccggca gcagcaggct gacgacggca agcaccataa
     3961 agcccaccgg caccgcgaag acaccggccg cgatcggctc caccccccac cagctgcatt
     4021 cggccagcgg gcgcgtgatg cccaggatgc cgcgcagcag gggcatgttg gtcgccatgt
     4081 agtagacgca gacgcccagc cccgccagca ttcccgcggt tgcgccggcg cgattggcgc
     4141 ggcgccagaa gatgcccagg atcaaggcgg ggaagaaggc cccggcggcc agagaaaagg
     4201 cggccgagac gaactgcagg atgtcggtga tgtgcagcga cgcgacatag gcagccgcca
     4261 gcgccaccac cagcaccatc accttggaca gcatcacgcg gcgtatcgcc gaggccttgg
     4321 ggttgagcac gcggtgatag aagtcgtgcg acagcgcgtt cgagatggtc agcagcagac
     4381 cgtccgccgt cgacaaggca gccgccaagc cgccggccgc caccagatag gtcacgacca
     4441 gcggcagtcc ggcaatctcg ggtgctgcca gcacgatcac gtcgccgccc aggtgcagct
     4501 cgcccagctg caggatgccg tcgccgttga cgtcctcgac cgacaccagc gaaggatcga
     4561 gcttggacca gcgcctgacc cagtccggca gctggtcata gggcaggccc acgacatgcg
     4621 agaagacctc gtacttgacc atcaccgcca gggccggcgc cgcaatgtag agcagcgaaa
     4681 tgaagaacaa ggaccaggcc accgaacgcc gcgcctcgcc gacgctgggc gtcgtgtagt
     4741 agcgggtcag cacatggggc atcgcggccg tgccgaccat gaggcagaag accagcgcca
     4801 ggaaattgcg gcgctcgcgt ttgaaggcct gctggtcttg agcatcgccc tcgccgaccg
     4861 cggcataggg tcgtgtctgg ggcgccagcc ccgccagcgg ctgtgcctgc agcacggcgc
     4921 tgtcgagttc gcgccgatag gccgccgcgg ccgcctcctg gctgcgcggc aaattcatga
     4981 gccggcgctc ggcctgctgg atgcgcgcga ggggcgcatc gtcggccttc attctcgcca
     5041 cctgggccgt gagcgcctgc gcgtcgcgct gcatcgctgc cggcacatcg gccaatttgc
     5101 ggcgcaaccc ctcggcacgc tggtgcaggt cggcactgac cgccagctcg cgcgggtcgg
     5161 cctgcagggc cgtctcgcgt tcgcccacct tggccaattg ctcgccatag gccaccacgg
     5221 ccaccggatt gccggtctgc ttggccgaga tccagaccac tggcaccagg taggcgacga
     5281 tgaggatgat gtactgggcc acctgggtcc aggtcacggc cctcatgccg cccaggaagg
     5341 agcacacaag cacgccgccc aggccgacga agatgccgat ctcgaaggtg aagccggtga
     5401 gatgagatgt gatcagcccc acgccgtaga tctgcaccac cacatagatg aaggacacca
     5461 gcaccgcgca ggccacgccg accaggcgcg gcagcacgcc gccatagcgg gcgccgagaa
     5521 agtcggggat cgtgtactgg ccgaagcgcc gcagataagg cgccagcagc agcgcgacca
     5581 ggcagtagcc cccggtccag ccgaggatgt aggccaggcc gccgaagccc ttcaggtaca
     5641 gcacacctgc cgtgccgatg aaggaggcgg ccgacatcca gtcggcggcg gtggccatgc
     5701 cgttgtagat ggccggcacg cgccgccccg cgacgtagta ttcgctctcg tcggtggtgc
     5761 ggctgagcag gccgatcacg gcgtagatgc ccaccgtcgc ggccaggaag ctggcgccta
     5821 tccagcctcg ggaccagccc aggcgctcga gcacgcccag gccgacgatg aagatgacca
     5881 cgccgacggt gaagatgccg tagcggcgat gcagcttgtg cgcgaagctg cgcgtgacct
     5941 gcgtcatggc tctcgcccgg cgggcccggg cgaggcgccc tgcccctcgc caccgtcgtc
     6001 atcctgagct gcgtaacgtg cgtcaagatg ctccatcgcg agggcataaa cggccacgat
     6061 ggcgacgaag agcagcaagg ccccctgcgc cgccatccag aagctcaacg ggaagtagcc
     6121 gacatgggcg gcacccaggc tggccgcgaa ccaggggccg acgaaggaca gcaggaacca
     6181 cagcagcagc agcacgccgg tcacgcgcag cacggcgcgc cagtaggcgc gctgcttggc
     6241 tgaaacggcg agagatacgc tgggcaccgg tcgattgtgc ggagcaaggc accgcgccgc
     6301 gactagagcg aaccctgcgc ggcggcggct cgcttggtgc tcagtgcctc agcgcttggc
     6361 taggcgctgc caggtctcga ccaccgagtc ggggttgagc gagatcgaga cgatgccctc
     6421 gctggccagc caatcggcga agtccgggtg atcgcttggc ccctggccgc agatgcccac
     6481 gtatttgccg actgcgcggc aggccgcaat ggcgcgggaa atcatggcct tgacggcggg
     6541 atcgcgctcg tcgaagtcgt gcgccagctg ctccaggccc gagtcgcggt ccaggcccag
     6601 cgtcagctgc gtcaggtcgt tggagcctat cgacatgccg tcgaagtgct ccaggaattg
     6661 ctcggccagg atggcattgc tcggcacctc gcacatcatg atgatgcgca ggtcgtcgtg
     6721 cccgcgcttc agcccatgct cgcccagcat ggcgatcacg cgctcggcct gtttgatggt
     6781 gcgcacgaag ggcaccatga tctcgacgtt ggtgagaccc atgccggtgc gcacgcgctt
     6841 gagcgcctcg cattccatcg cgaaggcctc gccgaactcc gggctgatat agcgcgaggc
     6901 accgcggaag cccagcatcg ggttttcttc ctcgggctcg tagcgcgagc cgccgatcag
     6961 cttgcggtat tcgttggact tgaagtccga caggcgcacg atcaccggct tgggccagaa
     7021 ggccgcggcg atggtggcga tgccctcggc cagcttgtcg acatagaagg cgcgcggcga
     7081 ggcatggccg cgcgccaccg actccaccgc cttcttcagg tcggcgtcga cgttcgggta
     7141 gtcgaggatg gccttgggat gcaccccgat gttgttgttg atgatgaact cgagccgcgc
     7201 caggcccacg cccgagttgg gcatctgcgc gaagtcgaag gcgagctgcg ggttgcccac
     7261 gttcatcatg atcttcacgg ggcagtaagg cagctcgccg cgctgcacct cgctgacttc
     7321 ggtttcgagc agaccatcgt agatgtagcc ggtgtcgccc tcggagcagg ccacggtgac
     7381 cagtgcgccg tccttcagcg tctcggtggc gttgccacag cccaccacgg ccgggatgcc
     7441 cagctcacgc gcgatgatgg ccgcatggca ggtgcggccg ccacggtttg tgacgatggc
     7501 cgaggcacgc ttcatcaccg gctcccagtt cgggtcggtc atgtcggtga ccagcacgtc
     7561 gcccggctgc acgcgctcca tctcggcgat gctgtgcacc agccgtacgg ggccggtgcc
     7621 gatcttctgg ccgatcgccc ggccctcggc gagcacggcg ccctggccct tgagcttgta
     7681 gcgatgctcg accttgccct cggcctgact cttcacagtc tcgggacggg cctgcaggat
     7741 gtagagctgg ccgtcgccgc cgtccttgcc ccattcgatg tccatcggcc ggccgtaatg
     7801 ctgctcgatg accagcgcgt atctggccag ctcggtcacg tcggcgtcgc tgagcgaata
     7861 gcggttgcgc agctcggccg gcgtgtccac cgtgcgtacc agcttgccgc tcgcggcctt
     7921 ctcgtcggca ccggcgaatt ccatcctgat gagcttggag ccgaggttgc ggcggatgat
     7981 gcctagcttg ccgttcttca gcgccggctt gtggacgtag aactcgtcgg ggttcacggc
     8041 gccctgcacc accgtctcgc ccaggccata gctggaggtg atgaagatca cgtccttgaa
     8101 gcctgattcg gtgtcaatcg tgaacatcac gccggccgcg cccagatcgg agcgcaccat
     8161 gcgctgcacg ccggccgaca gcgccacctc ggcatgcgca aagcccttgt gcacgcggta
     8221 gctgatggcg cggtcgttgt agagcgaggc gaacacctcc ttcatcttgt gcagcacctg
     8281 ctcgacgccc accacgttga ggaaggtctc ctgctggccg gcgaagctcg cgtcgggcag
     8341 gtcctcggcc gtggccgagg agcgcaccgc gaaggaagca tcggcattac cggccgtcag
     8401 cttcgcgaat tcggcacgga tcgccgcttc cagcgaggcc gggaagggct gcgcttcgac
     8461 ccagccgcgg atctgagcgc cggcttcggc cagcgcgcgt acgtcgtcgg tgttgagcgc
     8521 ggccaggcgc gcattgatgc gctccgccag accgccgtgc ttcaggaatt cgcggaaggc
     8581 atgcgccgtg gtcgcgaagc cgccgggcac tcgcacgccg gaggcggcga gctgcgagat
     8641 catttcgccg aggctggcgt tcttgccacc gaccacctcg acgtcggtca tcctcaagtt
     8701 ttcaaacggt acgaccaagg cggtcgcctc aaagcgggaa gacatgggaa agctccgtga
     8761 tgttgtgaaa ccggggctcc cgcgtcgcca aggcccgcag cgcgccaggc tggcgcgcag
     8821 gagacagccg acccaccgct tgtcgattct ggttgtgggg tggaaagctg catttggggg
     8881 gctgaaatcg cgagaaactg agccggattc tacgggctgg ccgcctatca tgggcgtgcc
     8941 tgcgatcccc tctattcaag cgccatgccc aaccgcaccg tgttcttcgt ttccgacggc
     9001 accggcatca cggccgaaac cttcggtaac tccatcctca accagttctc gggcaagccc
     9061 cgccacgtgc ggcgcccctt catcggcaca gccgacaagg cctaccaggt ggtgcgcgag
     9121 atcaatgaga cggctgagcg cgagggcaga aagccgatcg tcttcatcac gttggtgaac
     9181 gacgaggtgc tgaggatcgt ggtcgagcaa tccaggggcc tggtgctgga catgttcaag
     9241 accttcatcg agccgctgga gctggaactg ggcgtcaagt ccaatcaccg cgtgggtcgc
     9301 ttctccgacg tcgccaagag ccgggaatac gacaaccgca tcgaagccat caacttctcg
     9361 ctggcgcacg atgacgggca gtcagcgcgc aacctcgagc aggccgacgt gatcctggtc
     9421 ggcgtgagcc gcagcggcaa gacgccgacc tcgctctacc tcgcgatgca gcacggcgtg
     9481 aaggcggcca actacccgct gatcccggag gacttcgagc ggggcaagat cccgtcgtcg
     9541 ctcgcgccgc acaagaaaaa atgcttcggc ctgaccatcg atccggagcg cctcagctcc
     9601 atccgcaacg agcgccgccc gggcagcaaa tacgcgagcc tggagaactg ccgctacgag
     9661 gtgaatgagg ccgagcacat gatgaagcgc gagggcatct cctggctgtc gtcgacgcac
     9721 aagtcgatcg aagagatcgc aaccacgatc ctgcgagaca tcaggccgga tcggctgatt
     9781 tactgaacac ggccggacct gttgcaactc agcctgcaag ctgtgacgaa cttcacgcct
     9841 ggcccgcctg ccacgcaaac cgggggggcc ctgcgcattc gcgcttgcca cacaatttct
     9901 ctcggatgag ccgggccgca tagagggcag cccgggccga gccggaagct caccgcagga
     9961 gggcaagaca tgagccatac agcgtcgccg cgaaaccccg ccttcgacgg cctggatttc
    10021 caattgaccg atttcgatga ggcccgcgct gccctcgatg agctgcctcc aggaatcaac
    10081 gaggtccagc ggctgaagcc gggtttctgg gatgcccggc gccgcaagcc catggctacc
    10141 gatcgggccc tggccggctc cacgatggat tgggtcatgg gcttgacgcc agggctgcgc
    10201 ccgcgcgtcc tgtgcgagcg cttcccgcgc atcgccaacg cccttgcagc atgctggctc
    10261 gacgagcaac g
//
LOCUS       DASUCY010000381         2080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_169019, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000381 DASUCY010000000
VERSION     DASUCY010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169019"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..1179
                     /locus_tag="VFV25_07720"
     CDS             211..1179
                     /locus_tag="VFV25_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY1393249.1"
                     /translation="MSAQSTPHDYLQPLDCGITVIDTGFVRPRFDAAYLIVEQGRAAY
                     VDTGTNDALPRLLGALDAQGLSPDAVDWLIVTHVHLDHAGAAGALMRHLPRARLVVHP
                     RGARHMIDPRMLLDGARAVYGVEEVARTYGEVLPVPAERVLATADDMVLELADRPLRF
                     IDSPGHARHHHCIWDEASRGWFTGDTFGLSYPELETPVGRYILPTSTPVQFEPEALKA
                     SIRRMLSAQPQWMYLTHYGRVGAVPQLARLLLTQIDEMVALAKPLARSPARHDELKTA
                     FAGLYLEHLQRMDSPLTHERLRELLATDIELNAQGIGVWLDKPRTA"
     gene            1280..2065
                     /locus_tag="VFV25_07725"
     CDS             1280..2065
                     /locus_tag="VFV25_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY1393250.1"
                     /translation="MNAVNFALVGKVTVVTGAAQGIGEACVERFAHEGAALALWDVDD
                     ERGAVLEQRLHSQGVRAVYAHCDVSRKSEVDAAIARTLQAFGRIDVLVNNAGIFKAAD
                     FLDITEADWDAVINVNLKGSFLVGQACAREMAKTGGGAIVNMSSVNGIMAIPSIASYN
                     ASKGGINQLTRVMALALVDKGIRVNAVGPGTIATELAAKAVLTSDEARARIMSRTPMK
                     RLGEPGEIADIVAFLASDAASYMTGQIVYADGGRMSLNYTMPV"
BASE COUNT          371 a          707 c          672 g          330 t
ORIGIN      
        1 cttggggcgg cttggaatcg aggccccgtt ggtcaagaaa acatcgacca acagacggaa
       61 tgcagcttgt gcaatgcccg gctcaagagc gcccgggcgg gactggtgac gatgcaaaaa
      121 agaactcgcc cggatcaatg atgggcgagt tcaggctgaa ttgtcgcaca cctggggttt
      181 tcccgccagg gcccttccta gaatgcctca atgtcagccc agtccacccc gcatgactac
      241 ctgcagccgc tggattgcgg catcaccgtc atcgacaccg gcttcgtgcg cccgcgcttc
      301 gacgccgcct atctgatcgt ggagcagggg cgcgccgcct atgtggacac cggcaccaac
      361 gacgccctgc cccggctgct cggcgccctc gacgcccagg gcctgagccc cgacgctgtg
      421 gattggctga tcgtcaccca cgtgcatctg gaccatgccg gcgcggccgg cgcgctgatg
      481 cggcacctgc cccgcgccag gctggtcgtg catccgcgcg gtgcgcgcca catgatcgac
      541 ccgcgcatgc tgctggacgg cgcacgggcg gtctatggcg tcgaagaagt ggctcgcacc
      601 tatggcgagg tgctgccggt gccggccgag cgcgtgctcg caaccgccga cgacatggtg
      661 ctggagctgg ccgatcgccc tctgcgcttc atcgactcgc cgggccatgc gcgccatcac
      721 cattgcatct gggacgaggc aagccgcggc tggttcaccg gcgatacctt cggcctgtcc
      781 tatcccgagc tggagacgcc ggtcgggcgc tacatcctgc cgacttcgac gccggtgcaa
      841 ttcgagcccg aggccttgaa ggcttccatc cggcgcatgc tgagcgctca gccgcaatgg
      901 atgtacctca cgcattacgg tcgggtcggg gctgtgccgc agctcgcgcg tttgctgctg
      961 acgcagatcg atgaaatggt ggcgctggcc aagcccttgg cacgcagccc ggcgcgccac
     1021 gacgagctca agacggcctt tgccggcctc tatctcgagc acctgcagcg catggacagc
     1081 cccttgaccc acgagcgact gcgcgagctg ctggcgaccg acatcgagct caatgcccag
     1141 ggcatcggcg tctggctgga caagccgcga acggcctagc cctagctgat cctgcagacc
     1201 cgcgggacta cactttcccc tgcatcgcta gcgcccgccc acaaggcggg cttcttacat
     1261 cgcagaaggc aggcacaaca tgaatgcagt gaatttcgcg ctcgtcggca aggtcacggt
     1321 cgtcaccggc gcagcgcagg gcataggcga ggcttgcgtc gagcgcttcg cgcacgaagg
     1381 cgcggcgctc gcgctgtggg acgtggacga cgagcgcggc gcggtgctgg aacagcgcct
     1441 gcactcgcaa ggcgtgcgtg ccgtctacgc ccattgcgac gtttcgcgca agagcgaggt
     1501 cgacgcggcg atcgcacgca cgctacaggc cttcggccgc atcgatgtac tcgtcaacaa
     1561 tgccggcatc ttcaaagcgg cggatttcct cgacatcacc gaggccgatt gggacgcggt
     1621 catcaacgtg aacctgaagg gcagtttcct ggtcggccag gcctgcgcgc gcgaaatggc
     1681 caagaccggc ggcggcgcga tcgtcaacat gagctcggtc aacggcatca tggccatccc
     1741 cagcatcgcc agctacaacg cgagcaaggg cggcatcaac cagctgacgc gcgtgatggc
     1801 cctggcgctg gtcgacaagg gcatacgcgt caacgcggtg gggcctggca ccatcgccac
     1861 cgagctggcc gccaaggccg tgttgaccag cgacgaagcg cgcgcgcgca tcatgagccg
     1921 cacgccgatg aagcgcctgg gcgagcccgg cgaaatcgcc gacatcgtgg ccttcctggc
     1981 gagcgacgcc gccagctaca tgaccggcca gatcgtctat gcggacggcg gccgcatgag
     2041 cctcaactac accatgccgg tttaagggaa tcaggtcatg
//
LOCUS       DASUCY010000382         2389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_859653, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000382 DASUCY010000000
VERSION     DASUCY010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859653"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VFV25_07730"
     CDS             <1..412
                     /locus_tag="VFV25_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393251.1"
                     /translation="QAASCLVTGLGNLSRALVGLGHLERARGAAQECMRLAMGGGASS
                     RCACSLEPAVGLAVALGEHDRAVRFFSAAEANRHQLHLLREPADEGFIAPLVARAREA
                     LGEARYAAAFDSGLRMPFMDMFAEALHWLERAAH"
     gene            616..2226
                     /locus_tag="VFV25_07735"
     CDS             616..2226
                     /locus_tag="VFV25_07735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY1393252.1"
                     /translation="MCIAIWMTVGLFGRQPYKPDEAYTIGLVKSVVETGDWVVPRLVG
                     EPFMEKPSLFFVVAAGFSKWLPWLPMHEAARFAVVLFVAVGLWAIAACARELYGAGSG
                     RLAALLTLATVGTVARMHQLITDTGLFAGVALALLGLVKAPRQPGLAGVALGLGLAIA
                     FLTKGLLGPGLIVCTTLALLAHPAWRGSSMIRACMIATAIAVPLAACWLVPLYLRAPD
                     QLHVWFYDNNLGRFFGLNALGPKKDMLFYGRTLTWYAQPCLPLALWGWSRAVQGDRTA
                     PAQPGRAAPAAFLIVSLVVLTLASDGRELYALVLVPALAVAALDGLLALPIELERRLA
                     IGVAAVFGILAALLLCAYAAAVEWPAATARLPADIAMPGMVSASRDALALYAVITCAI
                     LVCLAPLRRPLRGALPLAGAAGIALLWATLVLPWGQYLDALKGYREVARSLEPHLPAA
                     GCIASRGLGEGERALLDYYIGLRTVRMETHPAAANQCSAVLIQVRSGETDNPPAAGTL
                     AWLGARPGSDNSSLRLYVYGAPAQVHDH"
BASE COUNT          317 a          823 c          818 g          431 t
ORIGIN      
        1 gcaggcggca tcatgcctgg tcacggggct ggggaacctt tcccgcgcgc tcgtcggcct
       61 gggccatctg gagcgcgcgc gcggcgcggc gcaagaatgc atgcgcctcg ccatgggggg
      121 cggcgccagc tcgcgctgcg cctgctcgct ggaaccggcc gtgggcctgg ccgtggcgct
      181 gggcgaacac gatcgggccg tccgcttctt ctcggccgcc gaagccaacc ggcaccagct
      241 ccacctcctg cgcgagcccg ccgacgaagg cttcattgcg ccgctggttg cccgggcgcg
      301 tgaggcattg ggcgaggcgc gctacgcggc agcgttcgac agcggcctgc gcatgccctt
      361 catggacatg ttcgccgaag cgctgcactg gctcgagcgc gcggcgcact gaccagtctg
      421 cccccactcc acgccggata ggcgcggcgt tgcacgccgt tgcggctcac acgtcaacac
      481 cgacatgtag gggcgaacgt tgttccgccg attgcaataa tcgacggtac cgcgactggc
      541 tcccccgata tgacagccat cggcctcgcc tcgcgaacgc cgcgagcatc ccatctgccg
      601 cgctggatcg ccgtgatgtg catcgccatc tggatgaccg tcgggctgtt cggccgccag
      661 ccctacaagc cggacgaggc ctacacgatc ggcctggtga agtccgtcgt cgagaccggc
      721 gactgggtcg tgcctcggct cgtcggcgag ccgttcatgg agaagccgtc actgttcttc
      781 gtcgtggccg caggcttctc gaagtggttg ccgtggctgc cgatgcacga ggcagcgcga
      841 tttgcggtgg tgctcttcgt cgccgtcggc ctgtgggcca ttgctgcctg cgcaagggag
      901 ctgtacggag cgggttcggg gcgcctcgcg gcgctgttga cattggccac ggtcggcacg
      961 gtggcccgca tgcaccagct gatcaccgac acgggtctgt tcgcgggcgt tgcgctggcc
     1021 cttctgggtc tcgtgaaggc gccgcgccag cccggcctgg ccggcgtagc gctgggcctc
     1081 gggttggcga tcgcctttct gacgaagggc ctgctgggcc ctggcctgat cgtgtgcacg
     1141 acgctggcat tgctcgcgca cccggcctgg cgcggcagct cgatgattcg tgcctgcatg
     1201 atcgccacgg cgattgccgt cccgcttgcc gcatgctggc tggtgccgct gtatctgcga
     1261 gcgccggacc agctgcacgt ctggttctac gacaacaacc tggggcggtt cttcggcctc
     1321 aatgcgctcg gcccgaagaa agacatgctc ttctacggcc gcacactgac ctggtatgcg
     1381 cagccgtgcc tgccgctggc gctgtggggt tggtcacgcg cggtgcaagg ggatcgcacg
     1441 gcgccggcgc agcccggcag ggcagcgccg gcagcgttcc tcatcgtgag ccttgtggtg
     1501 ttgacgctgg catcggacgg ccgcgaactc tatgccctgg ttctcgtgcc cgccctggcc
     1561 gtggcagcgc tcgatggttt gctggccttg ccgatcgagc tcgagcgccg gctggcgatc
     1621 ggcgttgccg ctgtgttcgg catcctggcg gcgctgctcc tttgcgccta cgccgctgca
     1681 gttgaatggc ccgctgccac ggcacgactg cctgccgaca ttgccatgcc cggcatggtc
     1741 agcgcatccc gagatgcgct tgcgctttat gccgtcatca cctgcgcgat cctcgtttgc
     1801 ctggcaccgc ttcgtcgacc gttgcgcggc gcgctgccgc tggccggcgc ggcaggcatc
     1861 gccttgctgt gggcgacgct cgtgcttccc tgggggcagt atctcgacgc gctgaagggc
     1921 tatcgcgagg tcgcccgctc gctcgagccg catcttccag ccgcagggtg catcgccagc
     1981 cgggggctgg gcgaaggcga gcgggctctc ctggactact acatcgggct gagaacggtc
     2041 cgcatggaaa cacatccggc ggccgcgaat cagtgcagcg cggtgctgat tcaggtgcgc
     2101 tcgggcgaga cggacaaccc gccggccgcc ggtacgcttg cctggctcgg cgctcgccct
     2161 ggaagcgaca actcgtcgct gcgcctgtac gtctacggtg cgccagcgca agtgcacgac
     2221 cattgaccgt cctcaacgcc cgctgaagcc gctcgcccga cactgcgagt tcccaatccc
     2281 ggttgggaga agcttccttg aggcataccc gcgtcatcgt cactcactac ggcgggcccg
     2341 atgcgctgcg ggtgatcgaa gaagagcgcc ccgagccgaa agatggcca
//
LOCUS       DASUCY010000383         5678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_506332, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000383 DASUCY010000000
VERSION     DASUCY010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 5678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5678
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506332"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..1269
                     /locus_tag="VFV25_07740"
     CDS             121..1269
                     /locus_tag="VFV25_07740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY1393253.1"
                     /translation="MNPWAVKAKAWLRKLRWWIALALAIVAWQAWSRATTPAPPPPPL
                     GEAKLADIVQTVQAAGTLQAKTRVDVGAQVSGQVKAIPVVLGNAVKKGDLLVLLDPEL
                     AQSDVARAEASVAQQRAQLEARRVDLEAARRELERQRRLLKGEATAATEAEKAENDVA
                     KLEVDVRGQDANLAQLQAQLERSKLNLAYTRITSPMDGVVVNIPVQVGQTVTAVQVTP
                     VMVTVADLDTITVRTKVPEADVQSLTPGQKASFTTLAGDGRRYEGRLRVIQPIPERAG
                     NAVFYNALFEVDNRDRKLFADMTVQVDIETGEARQTLAIPLVALGERGKDGAYSVEVA
                     GKDGKNDTRKIRTGLHDAANVQVLSGLKAGEKVLLAPPPAASAASAST"
     gene            1272..3281
                     /locus_tag="VFV25_07745"
     CDS             1272..3281
                     /locus_tag="VFV25_07745"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY1393254.1"
                     /translation="MALIELHGVERHYRLGDNDVAALAGVSLDIEAGEYVAIVGQSGS
                     GKSTLMNVLGCLDKPSAGHYRIAGRDTSALEPDELAALRREHFGFIFQRYHLLGHLDA
                     RANAALPAVYTGERAHVRLGRAHELLERLGLGERLHHKPNELSGGQQQRVSIARALMN
                     GGQVILADEPTGALDSASGAEMLRLLGELNARGHTVIIVTHDASVAAQARRVITMKDG
                     RVVSDADTPAGHVPAAPRFIQPARSTRLQRALVQWREAAQTAWAALRGSRLRTALSML
                     GISIGIASVVSIVALTSAARHSIESDVASFMSGRIAVWRGNSGLPPGSQPKPFLPAEI
                     DSLRGLPGVIGANLERQAQLTARQGSRDALIFTRSAAAQTLKARKLKIIEGRYFNPLD
                     LNDSAQVVVIDKKARDALFKKGEPPLGRSLMLTLGGTPDAAAAIATPGAAAPAWAALP
                     VTVIGVVDSDAAVLSGENWLGEVLIPEGTFSRKIDARADYDHFDLLLNPGTPPDRVRA
                     QIVHRLRALHGVEDFSTWNGEEEFRKFTNITASLAAVFAGVAAIALIVGGVGVMNIML
                     VSVSERTREIGIRMAVGARRSDVLLQFLVEAVLLCCLGGLVGVALSWLAALGFNHAQR
                     QLHVSVSWDALLVAFVVSSGVGLLFGTLPARRAAGLSPVDALARE"
     gene            3290..4681
                     /locus_tag="VFV25_07750"
     CDS             3290..4681
                     /locus_tag="VFV25_07750"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY1393255.1"
                     /translation="MRRLLRSAPFLSLLVAACASAPPEPPPEPAAPAQWTQIPSAGEA
                     AAWAPLLDPALQSLQQRTLAANTDIRRAALAWQAAQLQARLQGLRVQPSLSLSYSANR
                     PLKSQGATINVNGALVPVGETAQWTHSYSATAGAGFELDLWNRLAQLDAQQAALSEAA
                     RSDIAAAHQSIANRVAENYWTIAANQGFAAIASAKLEMAERAVPLVTARVREGKLVPL
                     EIDKAAAAVLAARQQLGQAEAGIAKARVSLAQLLDAPPPGPELAPVALPGELPAWLPD
                     EPAQVLERRPDVHRARLEVDAALAGARASRAARYPQLSFSAGLGTSGSQLSDWFAQPL
                     LSLAANLTVPLIDWRRLDLQDAQSRNALDRAALNLRDKVHGALAEVQGLLIEQRRIAD
                     ARAAADQQLAQARDAERIARLKLDVGAIPQADALQARIATLDAEQAVEQARLDALLNR
                     LALLGSLAVPPGA"
     gene            complement(4723..>5678)
                     /locus_tag="VFV25_07755"
     CDS             complement(4723..>5678)
                     /locus_tag="VFV25_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEY1393256.1"
                     /translation="RPLGGDDPPPPPREVGMMALPAFPSFLLIGGGAGLVLTLALLFV
                     AVAAGAYPVVRRLTRRLEVLKRGVETFGAGQLGHRVAVDGHDEVAAVASSFNHAAERV
                     EQLVRSNRSLLANASHELRSPLARLKMAVGLMEDAQPPQRDRLRQEIDRDIRELDALI
                     DEVLLASRLDARTRIERDSVDLLGLAAEEAARVDAVAGGEAIVVRGEERLLRRAFRNL
                     LENARRYGGDSAPEITVSRGFKGDAEVRVCDHGPGVPPEQRVRIFEPFYRLPGHAEHA
                     GGVGLGLSLVKQIAERHDGSVRCEGREPEQGSCFVISLPVG"
BASE COUNT          876 a         2044 c         1931 g          827 t
ORIGIN      
        1 aagtaggcct gaatgctcgc cgctgccgtc cccagacggc agggcgcaag cccagcacag
       61 cgaggctcgc gcccggcact ccctcgccat cgatcctcaa taacagtcag gaggaaacgc
      121 atgaatccat gggctgtaaa ggccaaggct tggctgcgca agctgcgctg gtggatcgcg
      181 ctcgcgcttg cgatcgtcgc ctggcaggcc tggagccgcg ccaccacccc agcaccccca
      241 ccgccgcccc tcggcgaggc taagctggcc gacatcgtcc agaccgtgca ggccgccggt
      301 accctgcagg ccaagacccg cgtggacgtt ggcgcccagg tcagcggcca agtcaaggcg
      361 attccggtgg tgctgggcaa tgcggtcaag aagggtgacc tgctggtgtt gctcgacccc
      421 gaactcgcgc agagcgatgt ggctcgcgcc gaggcctcgg tggcgcagca gcgcgcgcag
      481 ctcgaggcgc gccgggtcga tctcgaggcc gcgcgtcgcg aacttgagcg tcagcgccgc
      541 ctgctcaagg gggaggccac cgccgccacc gaagccgaga aggccgagaa cgacgtcgcc
      601 aagctcgagg tcgatgtgcg cggccaggac gccaacctag cccagctgca ggcccagctc
      661 gaacgcagca agctcaacct tgcctacacc cgcatcactt cgcccatgga cggcgtggtg
      721 gtcaacattc cggtgcaggt gggccagacg gtgactgcgg tacaggtcac gccggtcatg
      781 gtcactgtcg cagacctcga caccattacc gtgcgcacca aggtgcccga ggccgatgtg
      841 caatcgctca cgccgggtca gaaagccagc ttcactacct tggcgggcga cggccggcgc
      901 tacgagggcc ggctgcgcgt gatccagccc attccagagc gtgccggcaa cgcggtcttc
      961 tacaacgcgc tcttcgaggt ggacaaccgc gaccgcaagc tcttcgccga catgaccgtg
     1021 caggtcgaca tcgagaccgg cgaggccagg cagacactgg cgattcccct cgtcgcttta
     1081 ggcgaacgcg gcaaggacgg cgcctactcg gtcgaggtcg cgggcaagga cggcaagaac
     1141 gacacacgca agatccgcac cggcctgcac gacgcggcca atgtgcaggt gctctccggc
     1201 ctcaaggccg gcgagaaggt gctgctcgcg ccgccaccgg ccgcgtccgc agcctccgcc
     1261 tcgacctgac gatggcgctg atcgagctgc acggggtcga gcgccactac cggctcggcg
     1321 acaacgacgt ggccgccctg gccggcgtca gcctcgacat tgaggctggc gagtacgtgg
     1381 ccatcgtcgg ccagtcgggt tcgggcaaga gcacgttgat gaacgtgctg ggctgcctgg
     1441 acaagcccag cgctggccac taccgcatcg ccggccgcga caccagcgca ctggagccgg
     1501 acgagctcgc ggcgctgcga cgcgagcact tcggtttcat cttccagcgc tatcacctgc
     1561 tgggccatct cgacgcgcgc gccaacgccg cgctaccggc cgtctacaca ggcgagcgcg
     1621 ctcatgtgcg tcttgggcgc gcgcatgagc tgctcgagcg cctgggcctg ggcgaacgcc
     1681 tgcaccacaa gccgaacgaa ctcagcggcg gccagcagca gcgcgtcagc attgcgcggg
     1741 cgctgatgaa tggcggccag gtcatcctgg ccgacgagcc caccggcgcg ctcgactcgg
     1801 ccagcggcgc cgagatgctg cgactgctcg gcgaactcaa cgcgcgcggc cacacggtca
     1861 tcatcgtcac gcacgacgcc agcgtcgccg cccaggcgcg ccgagtgatc accatgaagg
     1921 atggccgtgt cgtcagtgac gccgacactc ctgcgggcca tgtaccggcc gcgcccaggt
     1981 tcattcagcc ggcgcgcagc acccgtttgc agcgtgccct tgtgcaatgg cgcgaggcag
     2041 cgcagaccgc ctgggccgcg ctgcgcggca gccggctgcg taccgccctt tcgatgctgg
     2101 gcatcagcat cggcatcgcc tcggtggttt ccatcgtggc gctgaccagc gcggcgcggc
     2161 attcgatcga gtccgatgtg gccagcttca tgtcgggtcg cattgcggtg tggcggggca
     2221 attccggcct gccgccgggc agtcaaccca aaccctttct tccggccgag atcgacagcc
     2281 tgcgcggcct gcccggcgtg atcggcgcca atctcgagcg ccaggcccag ctgacggctc
     2341 gccagggctc gcgcgacgcc ctgatcttca ctcgaagcgc cgcagcgcaa acgctcaagg
     2401 cacgcaagct caagatcatc gaggggcgct acttcaaccc cctggacctg aacgacagcg
     2461 cccaggtcgt cgtcatcgac aagaaggcac gcgacgccct gttcaagaag ggtgagccgc
     2521 cgctcggccg ttcgctgatg ctgacgctgg gcggcacgcc cgacgccgcc gcggccatcg
     2581 caaccccggg cgccgctgcg ccggcctggg ccgcgctgcc ggtcaccgtg atcggcgtgg
     2641 tggatagtga cgcagccgtg ctttcaggcg agaactggct cggcgaggtg ctaatacccg
     2701 agggcacctt ctctcgcaag atcgatgcgc gcgccgacta cgatcacttc gacttgctgc
     2761 tcaaccccgg cacgccgcct gatcgcgttc gcgcccagat cgtccaccgg ctgcgcgcat
     2821 tgcacggcgt cgaggacttc tcgacctgga atggcgaaga ggaattccgc aagttcacca
     2881 acatcaccgc cagcctcgcg gcggtgtttg ccggcgtggc cgccatcgcc ctaatcgtcg
     2941 gcggcgtggg ggtcatgaac atcatgctgg tctcggtctc ggagcgcacc cgcgagatcg
     3001 gcattcgcat ggccgtgggg gcgcggcgca gcgacgtgtt gctgcagttc ctggtcgagg
     3061 cggtgctgct gtgctgcctg ggcgggctcg tcggcgtggc cctgagctgg ctggccgccc
     3121 tcggtttcaa ccacgcacag cggcagctcc atgtctcggt gagctgggat gcactgctgg
     3181 tcgccttcgt cgtctccagc ggcgtgggcc tgctgttcgg caccttgccg gcgcgacgtg
     3241 cagcaggcct gagcccggtc gacgcgctag cccgcgaatg aggatcccca tgcgtcgatt
     3301 gcttcggtca gcgccattcc tttcgcttct ggtcgctgct tgcgccagcg cgcctcctga
     3361 gccgccgccc gagccggccg cgcccgcgca atggacgcag atcccgagcg ccggcgaagc
     3421 cgccgcctgg gcaccgttgc tcgacccggc cctacagtcc ttgcagcagc gtacgctcgc
     3481 cgccaacacc gatatccgcc gcgccgcgct ggcctggcag gcggcgcagc ttcaggcgcg
     3541 gctgcaggga ttgcgagtgc agccgtcgct gagtctgtcg tacagcgcga accggccgct
     3601 gaagtcgcag ggcgcgacga tcaacgtcaa cggcgccctg gtacccgtgg gcgaaacggc
     3661 gcaatggacc cacagctaca gcgccacggc cggcgccgga ttcgagttgg acctgtggaa
     3721 ccgcctggcc cagctcgatg cccagcaggc cgcgctgagc gaggccgcac gcagcgacat
     3781 cgccgcggcg caccaaagca tagcgaaccg ggtggccgag aactactgga cgatcgccgc
     3841 gaaccaaggc tttgctgcca tcgccagcgc caagctggag atggccgagc gggctgtccc
     3901 cctcgtcacc gctcgggtgc gcgagggcaa gctggtgccg ctcgagatcg acaaggccgc
     3961 cgcggccgtg ctcgcggcgc gccagcagct cggccaggcc gaggccggaa tcgccaaggc
     4021 gcgggtcagc ctggcgcaat tgctcgatgc gccgccgccc ggccccgagc ttgccccggt
     4081 ggcgctgcct ggggaattgc cggcgtggct gcccgacgag ccggcgcagg tgctcgaacg
     4141 ccggcccgac gtacaccgcg cgcgcctcga agtcgacgct gcgctggccg gcgcccgcgc
     4201 cagccgtgcg gcgcgttatc cgcagctgtc ctttagcgcc ggcctcggca ccagtggttc
     4261 gcagctcagc gactggttcg ctcagccgct gctgtccttg gccgccaacc tcaccgtgcc
     4321 gctgatcgac tggcgtcgcc tcgacttgca ggatgcccag tcgcgcaacg cgctcgaccg
     4381 cgcggcgctg aatctgcgcg acaaggtgca tggcgccctg gccgaggtgc agggcctgct
     4441 gatcgagcag cgccgtatcg ccgacgcgcg cgccgccgcg gaccagcagt tggcgcaggc
     4501 gcgcgacgcc gagcgcattg ctcgcctcaa gctcgatgtc ggagccatcc cccaggcgga
     4561 cgcgctgcag gcccgcatcg ccacgctcga tgccgagcag gcggtcgagc aggcccggct
     4621 cgacgcgctg ctcaatcggc tggctctgct gggctcattg gccgtgccgc cgggcgcttg
     4681 aggaggtggc tcgctgccct cttacgctct tacgcgatcc tctcagccca ccggcaagga
     4741 aatcacaaag cagctcccct gctccggctc ccgcccctcg cagcgcacgc tgccgtcgtg
     4801 gcgctcggcg atctgcttga ccaaggagag gcccaggccc acgccgccgg cgtgctcggc
     4861 atggccgggc aggcggtaga agggttcgaa gatgcgcacg cgctgttcgg gcggcacgcc
     4921 ggggccgtgg tcgcagacgc gcacctcggc atcgcccttg aagccgcggc tcacggtgat
     4981 ttccggggcg ctgtcgccgc cgtagcggcg cgcgttttcg agcaggttgc ggaatgcgcg
     5041 gcgcagcagg cgctcctcgc cgcgcaccac gatggcttca ccgccggcca ccgcatcgac
     5101 gcgggcagct tcctcggcgg ccagccccag caggtccacg ctgtcgcgct cgatacgggt
     5161 gcgcgcgtcg aggcggctgg cgagcagcac ttcgtcgatc agcgcgtcga gctcgcggat
     5221 gtcgcggtcg atctcctgcc tgagcctgtc gcgctgaggt ggctgtgcat cttccatcaa
     5281 gcccacggcc atcttgaggc gcgccagcgg cgagcgcagc tcgtggctgg cattggccag
     5341 cagcgagcgg ttcgagcgca ccagctgctc cacgcgctcg gccgcatggt tgaagctgct
     5401 ggccacggcg gcgacttcgt cgtgcccgtc gaccgcgacg cgatggccca gctggccggc
     5461 gccgaaggtc tcgacgccgc gcttgagcac ttcgagccgg cgcgtgagcc gccgcacgac
     5521 gggataggcc ccggccgcga cggcgacgaa gagcagcgcg agcgtgagca ccaggcccgc
     5581 cccgccgccg atcagcagga aggacgggaa ggccggcaag gccatcatgc cgacctcgcg
     5641 cgggggcggc ggtgggtcgt cgcccccaag aggccgtc
//
LOCUS       DASUCY010000384         2508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_586754, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000384 DASUCY010000000
VERSION     DASUCY010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586754"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..667
                     /locus_tag="VFV25_07760"
     CDS             152..667
                     /locus_tag="VFV25_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEY1393257.1"
                     /translation="MVKKLQKMAEKKSGSAAGLFDSQFAGTVKDSAQQIWLAGLGAFA
                     KAQEEGGKVFDALVKEGVAIQRKTQAVAEEKFGDVAGKMTKLSGEVQAKAGQHWDKLE
                     TIFEERVAKALNKLGVPSAKDVEALIKRIDELSAAVGKASAAKPAAARKKTAARPAAK
                     RSSRPARKSAA"
     gene            702..1901
                     /locus_tag="VFV25_07765"
     CDS             702..1901
                     /locus_tag="VFV25_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY1393258.1"
                     /translation="MASSSPRKHGRLHPRIGLALAGGGPLGAIYEIGALCALEEAVQG
                     LSFIDCEGYVGVSAGGFAAAGLANGMGPRELCRSFIENQGPDGDIFDPAALMRPAWGE
                     YAKRALQLPGLLAMVGLDYALGLVSRGTALSRLGSAMPTGLFSNDAVHEELSRIFSAP
                     GRSNDFRELKQRLVLVAVDLDSGEPRLFGAAGHDEVPISRAVQASAALPGLFPPVEID
                     GRHYVDGALRKTLHASVLLEQGLDLLICLNPLVPYGGSSDIGRLADGGLPQVLSQTFR
                     AIIRSRMELGMRGYERQYPDTDILLFEPDPLDTKIFFTNPFSYSQRRWLAEHAYQETR
                     KLLRERRGQIGATLARHGLMLKSAVLDDDRRSLVDPRPPAVGRANQALRRLETVLDEL
                     DRRLVAA"
     gene            2011..>2508
                     /locus_tag="VFV25_07770"
     CDS             2011..>2508
                     /locus_tag="VFV25_07770"
                     /inference="COORDINATES: protein motif:HMM:NF017770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HEY1393259.1"
                     /translation="MSSIPSALPAMRPAQTGAQQDHSTAPNPAAPRRTAYPIEFTASG
                     SEYFRIWIVNLLLTLVTVGLYYPWARVRKLRYFYGNTRIAGHAFDFHGNPRRMLRGYL
                     LVGALIALYSVAGKVSPTAGLVALVIVAAIWPALLRASMQFRLANTSWRGLRFRFTGS
                     TAEAYR"
BASE COUNT          420 a          861 c          840 g          387 t
ORIGIN      
        1 gctgcgcgct ttccccaagc ggcatagagc gcgcgcccta gagatttagg ctgccctgcc
       61 cagcgccgct tcttatagtt gcatcaggcg gcgtcagtag cgccgcagcg ttcgcaccac
      121 tcccgcaaac tcactctccc ggaaggcaat catggtcaag aagctccaga agatggcaga
      181 aaagaaatcc ggctcggccg ccggcctgtt cgacagccag ttcgccggca cggtgaagga
      241 ctcggcccag cagatctggc tggccggcct gggcgccttc gcaaaggccc aggaagaagg
      301 cggcaaggtc ttcgatgcgc tggtgaagga aggcgttgcc atccagcgca agacccaggc
      361 cgtggccgag gagaagttcg gcgacgtggc cggcaagatg accaagctct ccggcgaggt
      421 ccaggccaag gccggccagc attgggacaa gctcgagacc atctttgagg aacgcgtcgc
      481 caaggccttg aacaagctgg gtgtgccttc agccaaggac gtggaggcgc tcatcaagcg
      541 catcgacgag ctcagcgcgg ccgtgggcaa ggcctctgcc gcgaaaccgg ccgcggccag
      601 gaagaagacc gccgccagac ctgcagccaa gcgcagcagc aggccggcac gcaagtcggc
      661 tgcctgaaag ccagacgggc atcgctgcct acactggccg catggcttct tcatcgccga
      721 ggaaacacgg gagactgcac ccccgcatcg gcctcgcatt ggccggcggc ggacccttgg
      781 gcgcgatcta cgaaatcggc gcgctgtgcg ccctcgagga agccgtccaa ggcctgagct
      841 tcatcgactg cgagggctat gtcggcgtgt cggccggcgg cttcgcggcg gcggggctgg
      901 ccaacggcat ggggccgcgc gagctgtgcc gctccttcat cgagaaccaa ggtccagacg
      961 gcgacatctt cgaccccgcc gcgctgatgc gcccggcctg gggcgaatac gcgaagcgcg
     1021 cgctgcagct gccggggctg ctggcgatgg tgggcttgga ctatgcgctg ggcctggtct
     1081 cgcgcggcac ggccctcagc cggctcggca gcgcgatgcc gaccgggctg ttctcgaacg
     1141 atgccgtgca cgaagagctg agccgcatct tcagcgcgcc ggggcgcagc aacgacttcc
     1201 gcgagctgaa gcagcgcctg gtgctggtcg cggtcgatct ggacagcggc gagccgcggc
     1261 tcttcggagc cgccgggcat gacgaggtgc cgatctcgcg cgccgtgcag gccagcgccg
     1321 cgctgccggg gctctttccg cccgtcgaga tcgacggccg gcactacgtg gacggcgcgc
     1381 tgcgcaagac cctgcatgcc tcggtgctgc tggagcaggg cctggacctc ttgatctgcc
     1441 tcaacccgct ggtgccttat ggcggcagca gcgacatcgg gcggcttgcc gacggcggcc
     1501 tgccgcaggt gctgtcgcag accttccgcg ccatcatccg ctcgcgcatg gagctgggca
     1561 tgcgcggcta cgagcggcaa taccccgaca ccgacatcct gctgttcgag cccgatccgc
     1621 tcgacaccaa aatcttcttc accaacccct tcagctactc gcagcgccgc tggctggccg
     1681 agcatgccta ccaggaaacg cgcaagctgc tgcgcgaacg acgcgggcag atcggcgcca
     1741 cgctggcgcg gcatggcttg atgttgaaga gcgccgtgct cgatgacgat cgccgttcgc
     1801 tggtcgatcc gcgcccgcct gccgtgggcc gcgccaacca ggcgctgcgc cggctggaga
     1861 cggtgctgga tgagctggac cgtaggctcg tggcggcctg aggccggcgc acgcctcctc
     1921 ctttgggggg actggcgcac atcgcaagac agctgctaaa ttgcgcagcc acccacaaga
     1981 ttggttgttc ccctgggagg ggggatccgc atgagcagca ttccatccgc cttgccggcc
     2041 atgcggccgg cgcagaccgg cgcacaacaa gaccacagca cggcaccgaa ccccgccgca
     2101 ccgcgccgga cggcctaccc catcgaattc acagcctcgg gcagcgaata cttccgcatt
     2161 tggatcgtca acttgctgtt gacgctggtc accgtcgggc tgtactaccc ctgggcacgg
     2221 gtgcgcaagc tgcgctattt ctacggcaac acacgcatcg ccggacatgc cttcgatttc
     2281 cacggcaacc cccggcgcat gctgaggggc tatctgctgg tgggcgcact gatcgcgctg
     2341 tactcggtgg ccggcaaggt gtcgcccacg gccggcctgg tcgcgctggt gatcgtcgcg
     2401 gccatctggc cggcgctgct gcgcgcctcg atgcagttcc gcttggcgaa cacgagctgg
     2461 cgcggcctgc gcttccgctt cacgggcagc acggccgagg cctaccgc
//
LOCUS       DASUCY010000385         2086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_120905, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000385 DASUCY010000000
VERSION     DASUCY010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120905"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /gene="gltD"
                     /locus_tag="VFV25_07775"
     CDS             complement(<1..453)
                     /gene="gltD"
                     /locus_tag="VFV25_07775"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012668.1"
                     /note="glutamate synthase is composed of subunits alpha
                     and beta; beta subunit is a flavin adenine
                     dinucleotide-NADPH dependent oxidoreductase; provides
                     electrons to the alpha subunit, which binds L-glutamine
                     and 2-oxoglutarate and forms L-glutamate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HEY1393260.1"
                     /translation="MGKVTGFLEYERIEEGYAPVEQRLKHHKEFVIGLRGEEAKIQGA
                     RCMDCGTPFCNSGCPVNNIIPDFNDLVYRDDWPNAIRVLHSTNNFPEFTGRICPAPCE
                     AACTLNVNDDPVGIKSIEHAIIDRAWEEGWVQPQPPKARTGKRVAVVGS"
     gene            complement(478..>2086)
                     /locus_tag="VFV25_07780"
     CDS             complement(478..>2086)
                     /locus_tag="VFV25_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HEY1393261.1"
                     /translation="EDLAQLIHDLKNVNAKADISVKLVSEVGVGTIAAGVAKAKADHV
                     VIAGHDGGTGASPWSSIKHAGTPWELGLAETQQTLVLNRLRGRIRVQADGQMKTGRDV
                     VIGALLGADEFGFATAPLVVEGCIMMRKCHLNTCPVGVATQDPVLRRKFAGKPEHVVN
                     FFFFVAEEARQIMAQLGIRKFDDLIGRADLLDTRKGIAHWKAKGLDFGRIFHLPDAPA
                     EVARRQVETQGHGLAKALDLKLIEKCRPALDKGEKVQVLEEVRNVNRTVGAMLSGELV
                     RRYPEGLPDHTIFIQSEGTGGQSFGAFLARGITLYLIGDANDYTGKGLSGGRVVVRPS
                     IDFRGDATHNTIVGNTVLYGATEGEAFFRGVAGERFAVRLSGATAVVEGTGDHGCEYM
                     TGGTVLVLGKTGRNFAAGMSGGIAYVYDEDGQFAKRCNTAMVALEKLLPADEQRATQD
                     AAIFHRGVADEVLLRKLIEDHHRWTGSLRARDILDHWAEARAKFVKVFPHEYKRALSE
                     MNATKEADATIAKAKGSEKKGKAVPAK"
BASE COUNT          296 a          712 c          692 g          386 t
ORIGIN      
        1 ggagccgacg acggccacgc gcttgccggt cctggccttg ggcggctgcg gctgcaccca
       61 gccttcctcc caggcgcggt cgatgatcgc gtgctcaatg gacttgatgc ccaccggatc
      121 gtcgttgacg ttcagcgtgc aggccgcctc gcagggcgca gggcagatgc ggccggtgaa
      181 ttccgggaag ttgttggtcg agtgaagcac ccgtatcgca ttgggccagt cgtcgcgata
      241 cacgaggtcg ttgaagtccg ggatgatgtt gttgaccggg cagccgctgt tgcagaaggg
      301 cgtgccgcag tccatgcagc gggcaccctg gatcttcgcc tcctcgcccc tgaggccgat
      361 gacgaactcc ttgtggtgct tgagccgctg ctcgaccggc gcatagccct cctcgatgcg
      421 ctcgtattca agaaagccag tgacttttcc catgatgttc cttgtgctcg cagggcttca
      481 cttggccgga acggccttgc ccttcttctc gctgcccttg gccttggcga tggtcgcgtc
      541 ggcctccttg gtggcattca tttcgctcag ggcgcgcttg tattcgtgcg ggaatacctt
      601 gacgaacttg gcccgcgctt cggcccagtg gtccaggatg tcgcgcgcgc gcagcgagcc
      661 ggtccagcga tggtggtctt cgatcagctt gcgcagcagc acctcgtcgg ccacgccgcg
      721 gtggaagatc gccgcatcct gcgtcgcgcg ctgctcgtcg gcgggcagaa gcttctccag
      781 cgcgaccatc gccgtgttgc agcgcttggc gaactggccg tcctcgtcgt agacgtaggc
      841 gatgccgccc gacatgccag ccgcgaaatt gcgcccggtc ttgccgagca cgagcaccgt
      901 gccgcccgtc atgtattcgc agccgtggtc gccggtgcct tcgacgaccg ccgtggcgcc
      961 cgacaggcgc accgcaaaac gctcgcccgc cacgccgcgg aagaaggcct caccctcggt
     1021 cgcgccgtac agcacggtgt tgccgacgat ggtgttatgc gtcgcgtcgc cccggaagtc
     1081 gatgctcggg cgcaccacca cgcggccgcc ggacaggccc ttgccggtgt agtcgttggc
     1141 atcgccgatc aggtagagcg tgatgccgcg cgccaggaag gcgccgaagc tctgcccgcc
     1201 cgtgccttcg ctctggatga agatggtgtg gtcgggcagc ccctcggggt agcgccgcac
     1261 cagctcgccc gagagcatcg cgcccacggt gcggttgacg ttgcgcacct cctccagcac
     1321 ctgcaccttc tcgcccttgt cgagcgccgg ccggcatttc tcgatcagct tgagatccag
     1381 cgccttggca aggccgtggc cctgcgtttc gacctggcgg cgcgccacct cggccggcgc
     1441 atccggcagg tggaagatgc ggccgaagtc cagccccttg gccttccagt gcgcgatgcc
     1501 cttgcgggtg tcgagcaggt cggcgcggcc gatcaagtcg tcgaacttgc ggatacccaa
     1561 ctgcgccatg atctggcgcg cctcttcggc aacgaagaag aagaagttaa cgacatgctc
     1621 gggcttgccg gcgaacttgc ggcgcagcac cgggtcctgc gtggccacgc ccaccggaca
     1681 ggtgttgagg tggcacttgc gcatcatgat gcagccctcg acgaccagcg gcgcggtcgc
     1741 gaagccgaac tcgtcggcac ccagcagcgc gccgatcacc acgtcgcggc cggtcttcat
     1801 ctggccgtcg gcctgcacgc ggatgcggcc gcgcaggcgg ttgagcacca gcgtctgctg
     1861 cgtctcggcc aggcccagtt cccagggcgt gccggcgtgt ttgatgctcg accacggcga
     1921 ggcgccggtg ccgccatcgt ggccggcgat caccacatgg tcggccttgg ccttggccac
     1981 gccggccgcg atggtgccca cgccgacctc ggacaccagc ttgaccgaga tgtcggcctt
     2041 ggcgttgacg tttttcaggt cgtggatcag ctgcgcgagg tcttcg
//
LOCUS       DASUCY010000386         3391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1470242, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000386 DASUCY010000000
VERSION     DASUCY010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1470242"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="VFV25_07785"
     CDS             complement(<1..923)
                     /locus_tag="VFV25_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding protein"
                     /protein_id="HEY1393262.1"
                     /translation="MNQTRRTFTSSLSAVALSVCALGFAPLARAADTIKVGVLHSLSG
                     TMAISETVLKDTVLMTIDEINAHGGVLGKKLEPVVVDPASNWPLFAEKARQLISQDKV
                     AVVFGCWTSVSRKSVLPVFEELNGLLFYPVQYEGEELSKNVFYTGAAPNQQAIPAVEY
                     LMSKDGGGAKRFVLLGTDYVYPRTTNKILRAFLHSKGVADADIMEEYTPFGHSDYQTI
                     IANIKKFASEGKKTAVVSTINGDSNVPFYKELGNQGLKATDVPVVAFSVGEEELRGVD
                     TKPLVGHLAAWNYFMSVKNPTNDAFIKEWSA"
     gene            1151..2329
                     /locus_tag="VFV25_07790"
     CDS             1151..2329
                     /locus_tag="VFV25_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011480988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY1393263.1"
                     /translation="MFIRHRLTFLQQLLGEFPAVAILGPRQVGKTTLALELVRDQPSA
                     LYLDLESPADAAKLADPTAYFDAHADRLVVIDEVQRAPALFPALRGVIDRRRRAGRRV
                     GQFLLLGSATGALLAQSAESLAGRIAYVELQPLVTIEVPAEQAQRLWVRGGFPESFVA
                     ASDEASLRWRQQFIATYLERDIPQLGPRIPAETLRRLWTMLAHEQGQLVNAAKLAASL
                     AVSGQTVARYLDLLCDLMLVRRLRPWAANEGKRLVRTPKVYVRDSGVAHALLGLNDFD
                     DVIGHPVAGGSWEGWVIENLLAVAPPATQALFYRSSAGAEIDLVLELPKRQRWAIEIK
                     RSSAPAASRGFHIAADDLKVTARHVVHSGSESFPLTHGVRATTLADLQRALIELGPNR
                     "
     gene            complement(2366..3331)
                     /locus_tag="VFV25_07795"
     CDS             complement(2366..3331)
                     /locus_tag="VFV25_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY1393264.1"
                     /translation="MSPRPDPLLDPLAERGSADVVLIVDDVPDNLSLLHDALDESGYT
                     VLVATNGESALQRAAQARPDVILLDAVMPGMDGFEVARRLKADAQTSHIPIVFMTGLT
                     DTEHVVAAFEAGGADYVTKPLRPKEVLARIVTHVRSARHARQARNALDAFGHAAATVR
                     ASDGRLVWQTALARRLLDEYLGCRDAMLPDKAADWVRLQSSARRAGLDVPTLTLARGA
                     KRLVLALHEQTGDDEWMVVMREASDAAVVEALTLTFRLTQREAEVLYWLVKGKTNRDI
                     GDILGTSPATVKKHLEHVYEKLGVETRTAAAGAAIGKVRLLAAGG"
BASE COUNT          521 a         1157 c         1127 g          586 t
ORIGIN      
        1 taggcgctcc actccttgat gaaggcgtcg ttggtcgggt tcttgaccga catgaagtag
       61 ttccaggcgg cgaggtggcc gaccagcggc ttggtatcca cgccgcgtag ctcttcctcg
      121 ccgaccgaga aggccaccac gggcacgtcg gtcgccttca ggccctggtt gccaagctcc
      181 ttgtagaagg gtacgttgga gtcgccattg atggtcgaga ccacggccgt cttcttgcct
      241 tcggacgcga acttcttgat gttggcgatg atggtctgat agtcgctgtg gccgaagggc
      301 gtgtactcct ccatgatgtc ggcatcggcg acgcccttgg agtgcaggaa ggcgcgcagg
      361 atcttgttgg tggtgcgtgg gtagacgtag tcggtgccca gcagcacgaa gcgcttggcc
      421 ccgccgccgt ccttgctcat caggtattcc accgcaggga tggcctgctg gttcggcgcg
      481 gcgccggtgt agaacacgtt cttgctcagc tcctcgccct cgtactgcac ggggtagaag
      541 agcaggccgt tgagctcctc gaacaccggc agcaccgact tgcgcgacac gctggtccag
      601 cagccgaaca ccaccgcgac cttgtcctgt gagatcagct gccgcgcctt ttccgcgaac
      661 agcggccagt ttgatgccgg gtcgacgacg accggctcca gcttcttgcc gagcacgccg
      721 ccatgggcgt tgatctcgtc gatggtcatc agcaccgtgt ccttgagcac ggtctccgaa
      781 atggccatgg tgccggacag cgaatgcagg acgccgacct tgatggtgtc tgcggctcgc
      841 gccagggggg cgaagccgag cgcgcaaacg ctgagggcta cggcgctcag cgaggatgtg
      901 aaagtgcgac gggtctggtt cacggaaggt gctccttggt ctgtgtggcc gttgggattc
      961 acgcagagta gggaagccgt gggccgcagg aaatacgcca caaggcgtag gctgcagtca
     1021 aatggcgacg agcggatcag cggcgcgccg ccggtcggtt gggccgcagt ggagaccggt
     1081 tacccgggct cattcctcct tggtttgcta gaaccttgca atttctccac accatggaag
     1141 aattgcatgg atgttcatcc gccatcgcct cactttcctg cagcagctgc tgggcgaatt
     1201 cccggccgta gccatcctcg gcccgcgcca ggtgggcaag acgacactgg cgctggagct
     1261 ggttcgtgac cagccatcgg cgctgtatct ggatctcgaa tcacccgccg atgccgccaa
     1321 gcttgccgac ccgacggcgt acttcgatgc gcatgccgat cgccttgtcg tgatcgacga
     1381 agtgcagcgc gcacccgcgc tgtttcccgc gctgcgcggt gtgatcgaca gacggcgccg
     1441 tgccggccgg cgcgtcgggc agttcttgct gctgggctcc gccaccggcg ccttgctcgc
     1501 gcagtcagcc gagagcttgg ctggccgcat tgcctacgtc gagttgcagc cgctggtaac
     1561 gatcgaggta cccgccgaac aggcgcaacg cctgtgggtg cgcggcggct tccccgagtc
     1621 cttcgttgct gccagcgacg aagccagcct gcgctggcgc cagcagttca ttgccaccta
     1681 cctggagcgc gacatccccc agttgggacc ccgcatcccc gccgagacac tgcgccggct
     1741 gtggaccatg ctcgcgcacg aacagggcca gctcgtcaac gcggccaagc tcgcggcttc
     1801 gctcgcggtc agcggccaga ctgtcgcgcg ctacctcgac ctgctatgcg acctcatgct
     1861 ggtgcgccgg ttgcgaccct gggcggccaa tgaaggcaag cgcctggtgc gcacgcccaa
     1921 ggtctacgtg cgtgacagcg gcgtcgcgca tgcgctgctc ggcctcaacg acttcgacga
     1981 cgtgatcggg caccccgttg cgggcggcag ctgggagggc tgggtgatcg agaacctgct
     2041 cgccgtcgcg ccgccggcca cgcaagccct cttctaccgc agctcggcgg gcgccgagat
     2101 cgacctcgtg ctcgaactgc ccaagcgcca gcgatgggcg atcgagatca agcgcagcag
     2161 cgcaccggct gccagccgcg gctttcacat cgcggccgat gacctcaagg tgacggcgcg
     2221 ccatgtggtt cattcgggca gcgagtcctt tccgctgaca catggcgtac gcgcgaccac
     2281 gctggcggac ttgcagcgag cgctgatcga actcggaccg aaccgatgac gcggttcgcc
     2341 aagtgcatcc cactggaccg gggcgtcacc ctcccgccgc cagcagcctc accttgccga
     2401 tcgccgcgcc ggcggccgcc gtgcgcgtct ccacgcccag cttctcgtac acgtgctcca
     2461 ggtgcttctt caccgtggcc gggctcgtgc cgaggatgtc gccgatgtcg cggttggtct
     2521 tgcccttcac cagccagtac agcacctcgg cctcgcgctg cgtgaggcgg aaggtgagcg
     2581 tcagcgcctc caccacggcg gcgtcggacg cctcgcgcat caccaccatc cactcgtcgt
     2641 cgccggtctg ctcgtgcagc gcgagcacca ggcgcttggc gccgcgggcc agcgtgagcg
     2701 tcggcacgtc gaggccggcg cggcgggcgc tgctttgcag ccgcacccag tcggccgcct
     2761 tgtcgggcag catggcatcg cggcagccca ggtattcgtc gagcaggcgc cgcgccagcg
     2821 ccgtctgcca caccagccgc ccgtcgctgg cgcgcacggt ggccgcggca tgcccgaagg
     2881 catcgagcgc attgcgtgcc tgccgcgcgt ggcgggcact gcgcacatgc gtcacgatgc
     2941 gcgccagcac ctccttgggc cgcagcggct tggtcacgta gtcggcgccg cccgcctcga
     3001 aggccgccac cacgtgctcg gtgtccgtga gcccggtcat gaagacgatg gggatgtgcg
     3061 aggtctgcgc atcggccttc agccgccgcg ccacctcgaa gccgtccatg cccggcatca
     3121 ccgcatcgag cagaatcacg tctggcctgg cctgggcggc gcgctgcagc gcgctctcgc
     3181 cattggtggc caccagcacg gtgtagcccg attcgtcgag cgcatcgtgc agcagcgaca
     3241 ggttgtcggg cacgtcgtcg acgatcagca ccacgtcggc gctgccacgc tcggccagtg
     3301 gatcaagcag cggatcaggc cgtgggctca tgggtagacg caggacgaag cagccgggcc
     3361 accgcctcca gctggaagct gcgggccagg c
//
LOCUS       DASUCY010000387         2178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_490642, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000387 DASUCY010000000
VERSION     DASUCY010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490642"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /locus_tag="VFV25_07800"
     CDS             <1..1018
                     /locus_tag="VFV25_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HEY1393265.1"
                     /translation="AGLALGRFGARAVHEHLVRTSAWATVQAWRGSAPARAMASLVRE
                     RFPRVWAELEGLAEGLELPFGEVFLWNCRGDLWAMAPDGCTTVQLPGAAQQRIVHNED
                     GDPGFADHCALACCRIAGSPGFVSFVYPGSIAGHTFAVTDAGLAMTVNNLRALHVGPG
                     VPRMVLTRALLDASSLDAAVALLRAHPRAGGFHLTLGHRDSPQLLSVEFRSEDCSVLT
                     VEAAALHANHAIHPGMRDLPQLITGSSGHRQRRGAALIAEASSLQAPIDPLAILADDT
                     EPNFPILRLDPNDSDLENTLACADIVIHRTHVDWRVHENSRAPALFQLRNGQVQEGAA
                     PRGR"
     gene            978..1961
                     /locus_tag="VFV25_07805"
     CDS             978..1961
                     /locus_tag="VFV25_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEY1393266.1"
                     /translation="MDRFKKGQRPVADETRGSAVSGPPRSVEALRELTLRVGRGEGDV
                     SLGGKAHAVLAKLVERPEEVAVRTITELATVLGVNASTLTRLATRLGYEGFVDFQNVF
                     REGLAQRHRHFYSQQAERLVAAASARGPGHGDTPSREMQTVMQLARQSIDNVEGFVSQ
                     LVVDDLRGAARLLAEAPRVRVHGLRQFSTLAGFLAYGLGLIRSDVGLLDPQGLGAAEG
                     LAQLQRGDVVVVTSVEPYTQSVAAVAAAAAAAGLAVIAITDTRASPLVPPARHAFFVP
                     HESSYFSNSMGAYVVFCEALLNLVATRLGKGALQALERRERFITNLGIESD"
BASE COUNT          326 a          736 c          745 g          371 t
ORIGIN      
        1 agcgggcctc gcgctcgggc gcttcggtgc gcgcgccgtg cacgagcacc tcgtgcgcac
       61 atcggcctgg gccacggtgc aggcctggcg cggcagtgca ccggcgcggg caatggccag
      121 cctggtgcgc gagcgctttc cgcgcgtgtg ggcagagctg gagggtctgg ccgaaggcct
      181 cgagttgcct ttcggcgagg tctttctgtg gaactgccgt ggcgatctat gggcgatggc
      241 gccggacggg tgcacgacgg tgcagctgcc cggcgccgcc cagcaacgca tcgttcacaa
      301 cgaggatggc gatccgggat ttgccgacca ttgcgctctc gcctgctgcc gcattgcggg
      361 tagccccggc ttcgtctcgt tcgtctaccc gggctcgatc gccggccaca ccttcgccgt
      421 cactgacgcg gggctggcga tgaccgtcaa caacctgcgc gcgttgcacg tcggccccgg
      481 cgtgccgcgc atggtgctga cgcgggcctt gctcgatgca tcgagcctgg atgccgccgt
      541 ggcgctgttg cgcgcgcatc cccgcgccgg gggcttccac ctgaccctgg gccaccgcga
      601 cagccctcaa ctactgagcg tcgagttccg ctcggaggac tgttcggtcc tgactgtgga
      661 ggccgcggcc ctgcacgcca accacgcgat ccatccgggg atgcgcgacc tgccgcagct
      721 catcacgggc tcgtcggggc accgccagcg gcgcggcgcg gcgctcattg ccgaggcatc
      781 cagcctgcag gcgcccatcg acccgttggc gatcctggcc gatgacacgg agccgaactt
      841 tcccatcctg cgcttggatc cgaacgatag cgacctcgag aacaccttgg catgcgccga
      901 catcgtgatc caccggacgc acgtagactg gcgggtccac gagaattcac gcgcgccggc
      961 gcttttccag ctcaggaatg gacaggttca agaaggggca gcgccccgtg gcagatgaaa
     1021 cgaggggttc tgctgtgtcg gggcccccgc gcagcgttga agcactgcgt gaattgacgt
     1081 tacgcgtcgg gcgcggcgaa ggcgatgtca gcctcggcgg caaggctcat gccgtgctcg
     1141 cgaagctggt cgagcggccg gaggaggtcg ccgtgcgcac gatcaccgag ctggcgaccg
     1201 tgcttggcgt gaatgcgtcg acgctgacac gcctggccac gcgcctgggt tacgagggct
     1261 tcgtcgactt ccagaatgtg ttccgcgagg ggctggcgca gcgccaccgg cacttctaca
     1321 gccagcaggc ggagcgtctc gtggcagctg cgtccgcgcg tgggccgggc cacggcgaca
     1381 cgccctcgcg agagatgcag accgtcatgc aactcgcccg ccagtcgatc gacaacgtgg
     1441 agggtttcgt gtcccaactg gtcgtggacg accttcgcgg tgccgcgcgt ttgctggccg
     1501 aagcaccacg cgtgcgcgtt catggcctgc gccagttcag cacactggcc gggttcctcg
     1561 cctatggact cgggctgatc cgttcggacg tcggactgct agacccacaa gggcttggtg
     1621 cggccgaagg cctggcccaa ttgcagcgcg gcgatgttgt cgttgtgacc agcgtcgagc
     1681 cgtacaccca gagtgttgcc gcggtcgccg cggccgcggc cgcggcaggc ctcgccgtca
     1741 ttgcgatcac cgacacgcgc gcgtcgccgc tcgtcccgcc ggcgcgccat gccttcttcg
     1801 tgccgcacga gagcagctac ttcagcaaca gcatgggcgc gtatgtcgtc ttctgcgagg
     1861 cgctgctgaa tctcgtggcg actcggctgg gcaagggtgc attgcaggcc cttgaaagac
     1921 gagagcgctt catcacgaac ttggggatcg agtccgacta gggcccctaa cacagccgcg
     1981 aatgtcggcc accagcacga gccgcgttct tgggtggaca gattgctcgt ccacgcgctg
     2041 ttgattcagc ggcctggtca acgccaaccg gcacagtggt cagttgtggc cagcgcccgc
     2101 acttgccaaa gtcagtggag gctgccgccg agggtccgca gatcttcgcg cactgccagc
     2161 gccgtctcca gcgcaatg
//
LOCUS       DASUCY010000388         3059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_522809, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000388 DASUCY010000000
VERSION     DASUCY010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522809"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..1135)
                     /locus_tag="VFV25_07810"
     CDS             complement(176..1135)
                     /locus_tag="VFV25_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein/thiosulfate
                     sulfurtransferase GlpE"
                     /protein_id="HEY1393267.1"
                     /translation="MQQLVTLLIAHGVLLVFAVTLAARIGAPLPAAPLLVVAGGLAAG
                     GDLSWGATLLASIAANIAGDGLWFEAGRRHGYRVLRLLCRISLSPDACVRQSESLIAR
                     WGGSSLIAAKFLPGVSVVAAPMAGALRMPVARFLGYELAAALLWTGAYLLLGVVFSSQ
                     IQQVLDAIASAGMVATAALALLLLVWGAARYWRRRSALRDATMARITVGELQALMRDG
                     HDPVIIDVRSDAGTQVDARRIPGALLVPLERIHARAAELPRDREIVLYCNCPNEVSAV
                     RAAQLLAAQGLERVRPLAGGLDAWSASIEGTALTMAVGAAAGR"
     gene            complement(1274..2806)
                     /locus_tag="VFV25_07815"
     CDS             complement(1274..2806)
                     /locus_tag="VFV25_07815"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY1393268.1"
                     /translation="MGAPDLQRLQQHLHTSVGHWIAGRHQAPSSGRAQPVYNPATGDS
                     SRSVALASADEVNAAVAAAKAAFPAWAETPPIRRARVLFRFLELLNQHRDTLAAMITS
                     EHGKVFTDAQGEVTRGIEIVEFACGIPQLLKGDYTEQVSTGIDNWTMRQPLGVAAGVT
                     PFNFPCMVPMWMFPVAIACGNSFVLKPSERDPSPSLLMAELLKQAGLPDGVFNVVQGD
                     KLAVDTLLAHSDVKALSFVGSTPIAQYIYETGAHHGKRVQALGGAKNHMVVMPDADLD
                     QAVDALIGAAYGSAGERCMAISVAVLVGEVGDRIVPRLAERVHALKIMNGMELDAEMG
                     PIVTRQALDRISGYIAQGVQEGARLVVDGRGHKVAGHEQGFWLGGSLFDQVTPQMKIY
                     QEEIFGPVLVCVRAKDFSEAVQLVNDHEFGNGVACYTRDGHIAREFARRIQVGMVGIN
                     VPIPVPMAWHGFGGWKRSLFGDMHAYGEEGVRFYTKQKSVMQRWPESTAKGAEFVMPT
                     AK"
BASE COUNT          527 a         1039 c         1029 g          464 t
ORIGIN      
        1 tggccggccg cggccaggct gccggcaact gccagcagcg atgcggccgg cagcggtatc
       61 cccaggcgag ccacgaaggt gacgccgaac acgagcagcg cgccgtgcgc cgacagcgaa
      121 gccatcagct gctgcatgtc gggcatggag gccccgatac ggatcaggag cgcggtcagc
      181 ggcctgccgc ggcgccaacg gccatcgtca gcgcggtgcc ttcgatgctg gccgaccagg
      241 catcgaggcc gcccgccagc ggtcgcacgc gctcgaggcc ctgcgcggcc agcagctgcg
      301 cggcccgcac ggccgagacc tcgttggggc agttgcagta cagcacgatc tcgcgatcgc
      361 gcggcagctc ggcagcgcgc gcatggatcc gctccagggg cacgagcagc gcaccgggga
      421 tgcgccttgc gtcgacctgc gtgccggcgt cggatcgcac atcgatgatc accggatcat
      481 ggccgtcgcg catcagcgcc tgcagttcac cgacggtgat gcgcgccatc gtggcgtcgc
      541 gcaaggcact gcgccgccgc cagtagcgcg ccgcgcccca gaccagcaat agcagcgcga
      601 gcgcggcggt cgcgaccatg ccggcgctgg cgatcgcgtc gagcacctgc tggatctggc
      661 tgctgaagac gacgcccagc agcagatagg cgccggtcca cagcagggcc gcagcgagct
      721 cgtagcccag aaaacgcgcg acaggcatgc gcagggcgcc ggccatcggc gctgcgacga
      781 ccgagacgcc gggcaggaac ttcgcggcga tcagcgagct gccgccccag cgcgcgatca
      841 gcgattcgct ctgccgcacg caggcgtccg gcgacagcga gatgcggcac aacagcctca
      901 gcacacggta gccgtgccgg cgcccggcct cgaaccacag gccgtcgccg gcgatgttgg
      961 cagcgatgga agcgagcagc gtcgcgcccc aggacagatc ccccccagcc gcgaggccgc
     1021 cggcgaccac cagcaagggc gcggccggca acggcgcgcc tatgcgtgcc gccagggtca
     1081 cggcgaagac gagcagcacg ccgtgcgcga tcagcagggt caccagttgt tgcatatcga
     1141 ggaaggtgga gcttatagct gccggatcaa gcctatgcgg cttgaggcta tctcagtatt
     1201 gcgcaccaca tcgtgactcc caaatagccc gaaccccggc agggcgtcgc aggcgctacg
     1261 ggggcgggtg tcatcacttc gccgtcggca tcacgaactc ggcccccttg gccgtgctct
     1321 cgggccagcg ctgcatcacg ctcttttgct tggtgtagaa gcgcacgccc tcctcgccat
     1381 aggcgtgcat gtcgccgaac aaggagcgtt tccagcctcc gaagccatgc caggccatgg
     1441 gcaccgggat gggcacgttg atgcccacca tcccgacctg gatgcggcgc gcgaattcgc
     1501 gggcgatgtg gccgtcgcgc gtgtagcagg ccacgccgtt gccgaattca tggtcgttga
     1561 cgagctgcac cgcctcgctg aaatccttcg cgcgcacgca gaccagcacc ggtccgaaga
     1621 tttcctcctg atagatcttc atctgcggcg tcacctgatc gaacaagctg ccgccgagcc
     1681 agaagccctg ctcatggccg gccaccttgt gaccgcggcc gtcgaccacg agcctggcgc
     1741 cctcctgcac gccctgggcg atgtagccgc tgatgcggtc cagcgcctgg cgcgtcacga
     1801 tggggcccat ctcggcgtcg agctccatgc cattcatgat cttgagcgcg tggacgcgct
     1861 cggccaggcg cggcacgatc cggtcgccca cctcgccgac cagcaccgcg acgctgatcg
     1921 ccatgcagcg ctcgccggcc gagccgtaag cggcgccgat cagcgcgtcc accgcctggt
     1981 cgagatcggc gtcgggcatc acgaccatgt ggttcttcgc gccgcccagg gcctgcacgc
     2041 gcttgccgtg atgggcgccg gtctcgtaga tgtactgcgc gatcggcgtg gagccgacga
     2101 agctcagcgc cttcacgtcc gaatgcgcga gcagcgtgtc tactgccagc ttgtcgcctt
     2161 gcacgacgtt gaagacgccg tcgggcaggc cggcctgctt gagcagctcg gccatcagca
     2221 gcgacggcga aggatcacgc tcgctgggct tgagcacgaa gctgttgccg caggcgatgg
     2281 ccaccgggaa catccacatc ggcaccatgc aggggaagtt gaagggcgtg acgccggccg
     2341 ccacgcccag cggctggcgc atggtccagt tgtcgatgcc agtggagacc tgctcggtgt
     2401 aatcgccttt gagcagctga ggaatgccgc aggcgaactc gacgatctcg atgccgcgcg
     2461 tgacctcgcc ctgggcgtcg gtgaagacct tgccgtgctc gctggtgatc atggcggcca
     2521 gcgtgtcgcg atgctggttg agcagctcta ggaagcgaaa cagcacgcgt gcgcggcgaa
     2581 tcggcggcgt ctcggcccag gccgggaaag ccgccttggc cgcggcaaca gcggcattca
     2641 cctcatcggc gctggccagc gcgaccgagc gcgaactgtc gccggtggcg gggttgtaga
     2701 ccggctgggc gcgaccgctg ctcggagcct gatggcgccc ggcgatccaa tggccgacgc
     2761 tggtatgcaa atgctgctgt aaacgctgca gatcgggtgc gcccatgtcc tgtctccgtg
     2821 tgcgatggag ccagtctagt gcttatggac agcggacatc cgatccggcc cctgcggaga
     2881 aaaaaaagcc cacccaaaag actgggtggg ctttcggaag tcctcaaaaa aaggacgtcg
     2941 ttgggagcct gtagggagca acgatctgag tcgcggttta cttgcctctt gctcgccctt
     3001 taagtttgtt caaccctgaa tgtttcgttg atcacgaaat gccgtgagac agcagtgcc
//
LOCUS       DASUCY010000389         4240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_233342, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000389 DASUCY010000000
VERSION     DASUCY010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233342"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..2971
                     /gene="infB"
                     /locus_tag="VFV25_07820"
     CDS             122..2971
                     /gene="infB"
                     /locus_tag="VFV25_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEY1393269.1"
                     /translation="MAVTTVAQFAAELNRPATTLLEQLQSAGVSKTSTQDALTEADKE
                     RLLEFLRSTHGSAGGSDRKKITLTKKSTSEIKQADASGKARTIQVEVRKKRVFIKRDE
                     VATVEEAAPKAPVVDEAELQRREEEASRQAELLRRQEEELAEKRRQREEQERSEREAA
                     EARAREAAERAAAELAAAAAAKQEVAAPAPAAVAAAQPAPAPAPVAKPVARVVKAADI
                     EAEEKQRLGDLEKRRKAAEAEAAAIRAMMAAPKRVLTAKKPEEDKPKAAEAGKEGIKG
                     TIHKPKAAPGAAGAAAPAGAKPGDKKSIKSEKLSSSWADDAKKRGALKTRNDAPAGAR
                     GGWKAPRGGRRGERTEGNGASSFTPPVEAQIYEVHVPETISVADLAHKMSVKASEVIK
                     QLMKLGQMVTINQQLDQETAMILVEEMGHKAFAAKLDDPEAFLQLDEAGEQQAEALPR
                     APVVTVMGHVDHGKTSLLDYIRRSRVAAGEAGGITQHIGAYHVETPRGVITFLDTPGH
                     EAFTAMRARGAKATDIVILVVAADDGVMPQTKEAIHHAKAAGVPLVVAINKIDKPDSN
                     LERVKSELVAEQVVPEEFGGDSPFVPVSAKTGQGVDDLLEQVLLQAEVLELKAPVEAP
                     AKGLVIEAQLDKGRGPVATVLVQSGTLKRGDVVLAGSSYGRVRAMLDEDAKSATEAGP
                     SIPVEIQGLTEVPQAGDEFMVLADERRAREIATFRSGKYRDVKLAKQQAAKLENMFEN
                     MGQGEAQTLALIIKADVQGSQEALSASLLKLSTAEVKVQVVHAAVGGISESDVNLAIA
                     SKAVIIGFNVRADAGARKLAEGNAVDIRYYNIIYDAVDEIKAAMSGMLAPEQKEEVIG
                     TAEIRTVFVASKIGTVAGSMVTSGVVRRNARFRLLRDHVVIYTGEVESVKRMKDDVRE
                     VSEGFECGIKLKNYNDIAEGDQLEFFEVKEVARTL"
     gene            3066..3431
                     /gene="rbfA"
                     /locus_tag="VFV25_07825"
     CDS             3066..3431
                     /gene="rbfA"
                     /locus_tag="VFV25_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEY1393270.1"
                     /translation="MRHKQKSIPNRSFRVADQIQRDLAELIRELKDPRIGMVTINAVE
                     VTPDYAHAKVFFSLLVGDTQECEQGLNEAAGYLRNGLFKRLQIHTVPTLHFSFDRTTE
                     RAAEMSALINKANADRAKE"
     gene            3428..>4240
                     /gene="truB"
                     /locus_tag="VFV25_07830"
     CDS             3428..>4240
                     /gene="truB"
                     /locus_tag="VFV25_07830"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEY1393271.1"
                     /translation="MKPAQKPRLPRRALHGVLLLDKPLGWSSNDALQKVKGMLRAEKA
                     GHTGTLDPLATGLLPLCFGAATKFSQVSLDADKAYRATLQLGVTTTTGDAEGEVLERR
                     QVDVSGDKLQRACAALTGEIDQLPPMHSALKHEGKALYEYARAGVEIERAPRRVTIHR
                     LDIVSWQADELVLDVHCSKGTYVRTLAEDLGRALGCGAHLSALRRTACGPLRVEDAVS
                     IEALAEMTEAERWALLRPPDCLLAAWPEVRLAAGEAGRFLSGLRRRVSLSDSA"
BASE COUNT          823 a         1392 c         1471 g          554 t
ORIGIN      
        1 ggcgtggagc aggccaaggc gctgatcatg aaggcccgcg aacactggtt cactgcctga
       61 tcgccacgca gcagcccgac tcgcagacgc ccaagaacgc ataccgcaag gactacacac
      121 aatggccgtg accaccgtcg cacagttcgc cgccgagctg aaccgcccag cgacgacact
      181 gctcgagcag ctccaatccg ccggcgtcag caagacgtcg acgcaggatg cgctcaccga
      241 agcggacaag gagcgcttgc tggagttctt gcgttccacg cacggctccg ccggcggcag
      301 cgaccgcaag aagatcacgc tgaccaagaa gtcgacctcc gagatcaagc aggccgacgc
      361 atccggcaag gcgcgcacca tccaggtcga ggtacgcaag aagcgcgtct tcatcaagcg
      421 cgacgaagtg gctactgtcg aagaggccgc acccaaggcg cctgtggtcg atgaagccga
      481 actgcagcgc cgcgaggaag aagccagccg ccaggccgag ctgctgcgcc gccaggaaga
      541 agagctggcc gagaagcgcc gccagcgaga ggaacaggag cgcagcgagc gcgaagccgc
      601 ggaagcccgg gcgcgcgaag cggccgagcg cgccgccgcc gaactggcgg ccgctgccgc
      661 ggccaagcag gaagttgccg ctcccgcgcc ggctgccgtt gcagccgccc agccagcccc
      721 ggcccccgcc ccggtcgcca agcccgtagc gcgcgtcgtc aaggcggccg acatcgaggc
      781 cgaagagaag cagcgcctgg gcgatctgga gaagcgccgc aaggccgccg aggccgaggc
      841 cgccgcgatc cgcgccatga tggccgcgcc caagagggtg ctgacggcca agaagccgga
      901 agaggacaag cccaaggcag ccgaggccgg caaggaaggc atcaagggca ccatacacaa
      961 gcccaaggca gcgcccggcg cggcgggggc agcggctccg gccggtgcca agcccggcga
     1021 caagaagtcg atcaagtccg agaagctctc gtccagctgg gccgacgacg ccaagaagcg
     1081 cggcgccctg aagacgcgca acgatgcgcc cgccggcgca cgcggcggct ggaaggcccc
     1141 gcgcggcggg cggcgcggcg aacgcactga aggcaatgga gcgagcagct tcacgccgcc
     1201 ggtcgaggcc cagatctacg aggtgcatgt gcccgagacc atctcggtgg ccgacctggc
     1261 gcacaagatg tcggtcaagg cctccgaggt catcaagcag ctgatgaagc tgggccagat
     1321 ggtcaccatc aaccagcagc tcgaccaaga gaccgcgatg atcctggtcg aggaaatggg
     1381 ccacaaggcc ttcgcggcca agctggacga cccggaagct ttccttcagc tggacgaggc
     1441 cggcgagcag caggccgagg ctctgccgcg cgctccggtg gtcacggtca tgggccacgt
     1501 ggaccatggc aagacctcgc tgctggacta catccgccgc agccgcgtcg ccgcgggcga
     1561 agcgggcggc atcacgcagc acatcggcgc ctatcacgtc gagacgccgc gcggcgtgat
     1621 caccttcctc gacacgccgg gccacgaggc cttcacggcc atgcgcgcac gcggtgccaa
     1681 ggccaccgat atcgtgatcc tggtggtggc ggccgacgat ggcgtaatgc cgcagaccaa
     1741 ggaggcgatc catcacgcca aggctgccgg cgtgccgctg gtcgtggcga tcaacaagat
     1801 cgacaagccc gattccaacc tggagcgcgt caagagcgag ctggtggcgg aacaggtcgt
     1861 gcccgaggaa ttcggcggcg actctccctt cgtcccggtg tcggccaaga cggggcaagg
     1921 cgtcgatgac ctgctggagc aggtgctgct gcaggctgag gtgctggagc tgaaggcgcc
     1981 ggtcgaagcg ccggccaagg gcctggtcat cgaagcccag ctcgacaagg gccgcggccc
     2041 ggtcgcgacg gtgctggtgc agtccggcac gctcaagcgc ggcgacgtgg tcttggcggg
     2101 ttccagctat ggtcgcgtgc gcgccatgct cgacgaagat gccaagtccg cgaccgaggc
     2161 cggcccgtcg attccggtcg agatccaggg cctgaccgaa gtgccacagg ctggcgacga
     2221 gttcatggtg ctggccgacg agcggcgcgc gcgcgaaatc gcgaccttcc gttccggcaa
     2281 gtaccgcgac gtcaagctgg ccaagcagca ggctgccaag cttgagaaca tgttcgagaa
     2341 catgggccaa ggcgaagcgc agactctggc cttgatcatc aaggccgacg tgcaaggctc
     2401 gcaggaggcg ctgtcggcct cgctgctcaa gctcagcacc gccgaggtca aggtgcaggt
     2461 cgtacacgcg gctgtcggcg gcatcagcga gagcgacgtc aacctggcca tcgcctccaa
     2521 ggccgtcatc atcggcttca acgtccgagc ggatgcaggt gcacgcaagc tggccgaggg
     2581 caatgcggtc gacatccgct actacaacat catttacgac gccgtcgacg agatcaaggc
     2641 ggcgatgtcg gggatgttgg cgccggagca gaaggaagaa gtcatcggca cggcagagat
     2701 ccgcaccgta ttcgtggcgt ccaagatcgg cacggtggcc ggctccatgg tcacctcggg
     2761 cgtggtgcgc cgcaatgcgc gcttccgatt gctgcgcgac cacgtggtca tctacacggg
     2821 tgaagtcgag tcggtcaagc gcatgaagga cgacgtgcgc gaggtcagcg agggcttcga
     2881 gtgcggtatc aagctgaaga actacaacga catcgcagag ggcgatcagc tggaattctt
     2941 cgaggtcaag gaagtggccc gcacgctgta aggcgggcag gccatcggcc gcaaaccccg
     3001 ccgatcgcaa gacggcgggg tttgtcgttg ggggcttggc gcccggcaat gccaggagtt
     3061 gcaccatgcg acacaagcaa aaatcgattc ccaaccgcag cttccgtgtg gccgaccaga
     3121 tccagcgcga cctggccgag ctgatccgcg agctgaagga tccgcgcatc ggcatggtga
     3181 cgatcaatgc cgtcgaggtg acgcccgact acgcccatgc caaggtgttc ttttcgctgc
     3241 tggtgggtga cacgcaggag tgcgagcagg ggctgaacga ggccgccggc tatctgcgca
     3301 acggcctgtt caagcggctg cagatccaca ccgtgccgac gctgcatttc agcttcgacc
     3361 gcaccactga gcgcgcggcc gagatgagtg ccttgatcaa caaggccaac gccgatcgag
     3421 ccaaagagtg aagcccgcgc agaagccccg ccttccgcgt cgtgcgctgc acggcgtgct
     3481 gctgctggac aagccgctgg gctggtccag caacgacgcg ctgcagaagg tcaagggcat
     3541 gctgcgcgcc gagaaggccg gccacaccgg cacgctggac ccattggcca ccggcttgct
     3601 gccgctgtgt ttcggcgcgg caaccaagtt ctcccaggtc agcctggacg ccgataaggc
     3661 ctaccgtgcg acgctgcagc tgggcgtgac cacgaccacg ggcgacgccg aaggcgaggt
     3721 gctggagcgc cgccaggtcg acgtgagtgg cgacaagctg cagcgcgcct gcgccgcgct
     3781 cactggcgag atcgaccagc tgccgcccat gcattcggcg ctcaagcacg agggcaaggc
     3841 gctgtacgag tacgctcggg ccggcgtcga gatcgagcgt gcgccgcgcc gcgtcacgat
     3901 tcatcgtctc gacatcgtga gctggcaggc tgacgagctg gtgctcgatg tgcactgcag
     3961 caagggcacc tatgtacgta ccctggccga agacctgggc cgagcgctgg gctgcggggc
     4021 gcacttgagc gcgctgcggc gcaccgcctg cggcccgctg cgcgtcgaag atgccgtgag
     4081 catcgaggcg ctggccgaga tgacggaagc cgagcgctgg gcgctgctga ggccgccgga
     4141 ctgcctgctg gcggcttggc ccgaggtgag gctggccgcc ggcgaagccg ggcgctttct
     4201 gtccggcctg cgccgccgcg tgagcctgtc cgacagcgcc
//
LOCUS       DASUCY010000390         2038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1325861, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000390 DASUCY010000000
VERSION     DASUCY010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325861"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VFV25_07835"
     CDS             complement(<1..453)
                     /locus_tag="VFV25_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HEY1393272.1"
                     /translation="MALVEVHSQELKLYPPPEAAVKAAHVSGMEAYRKLAAEAEADYE
                     GYWARLARELVSWKQPFTRVLDESNAPFFKWFEDGTLNVSYNCLDRNVERGVGDKTAI
                     IFEADDGKVTRVSYKELLARTCQLANALKGLGVKKGDRVVIYVPMSIEG"
     gene            complement(593..2005)
                     /locus_tag="VFV25_07840"
     CDS             complement(593..2005)
                     /locus_tag="VFV25_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEY1393273.1"
                     /translation="MDALAPKDVLSELWLGVGGAPEALAAVELSGAEPALPSSFAVGT
                     AAQASIAAAALAARELGRLRGGPAQQVRVDMQHAAAEFRSERYLRIDGAAPPDPWDRI
                     AGAYRCGDGRWVRLHTNFPHHRDGVLRLLGCDYDRAAVAQALLGWEAERFEEAAAQAK
                     MVVAAMRSFEEWDASPQGQALAGLPLVSIERIPGGDAPSQPLPSLADGARPLSGVRVL
                     DLTRIIAGPVCGRTLAAHGAEVLLVTSPRLPSVPTLVIDTGRGKRTTQLDLTEADDRA
                     ALAALAGEADVFVQGYRPGGLSEQGFGPEDLARLRPGIVYVSLSAYGHAGPWATRRGY
                     DSLVQTASGFNAAEAEAAGSSEPKVLPMQALDHASGYLLALGAQIGLLRRATQGGSWH
                     VRVALARTGLWLRSLGRLPQGLAAHDPGFEGSQDWLETSDSGFGRMTAVRHAGILSAT
                     PPAWTAPAMPLGSHAPRWSS"
BASE COUNT          301 a          748 c          677 g          312 t
ORIGIN      
        1 gccctcgatc gacatcggca cgtagatgac gacgcggtcg cccttcttca cgcccaggcc
       61 cttcagcgcg ttggccagct ggcaggtcct ggcgagcagc tccttgtagc tcacgcgggt
      121 gaccttgccg tcatcagcct cgaagatgat ggccgtcttg tcgcccacgc cacgctcgac
      181 attgcggtcc aggcagttgt acgagacgtt gagcgtgccg tcctcgaacc acttgaagaa
      241 gggcgcattg gattcgtcca gtaccctggt gaaaggctgc ttccagctca ccagctcgcg
      301 cgccaaccgc gcccagtagc cctcatagtc agcctcggcc tcggctgcca gcttccggta
      361 ggcctccatg cccgagacat gggccgcctt cacggccgcc tcgggcggcg ggtagagctt
      421 gagttcttgc gagtggactt ccacgagggc catgacttgt ctccttgatg tggtgatatt
      481 cgggacgcta gtgccgcacc ataaggacca gctcttacga tccccttact cccgatagtt
      541 tctctcgcgc ggcgcttgct tcggctttcg aacgcccgtt cgctgcgggt ctttacgagc
      601 tccagcgcgg cgcatggctg cccaagggca tggcaggcgc cgtccacgcc ggcggcgtgg
      661 cgctcaggat gcccgcgtgg cgcaccgcgg tcatccggcc gaagcccgaa tcgctggttt
      721 ccagccagtc ctgcgagccc tcgaagccgg ggtcgtgggc cgccaggccc tgcggcaggc
      781 gccccaggct gcgcaaccag agccccgtgc gcgccagcgc gacccggaca tgccagctgc
      841 cgccctgcgt ggcccgtcgc agcagaccaa tctgagcgcc aagcgccagc agatagccgg
      901 aggcgtggtc cagcgcctgc atcggcagca ccttgggctc ggaggaccct gccgcctcgg
      961 cctcggcggc attgaagccg ctcgcggtct gcaccagcga gtcatagccg cgccgggtcg
     1021 cccagggccc ggcatggcca taggcgctca ggctcacata gacgatgccg ggccgcagcc
     1081 gcgccagatc ctcgggcccg aagccctgct cgctcaggcc gccggggcgg tagccctgca
     1141 cgaagacatc ggcctcgccg gccagcgcgg ccagagcggc gcgatcgtcg gcctcggtca
     1201 gatcgagctg ggtcgtgcgc ttgccgcggc cggtgtcgat gaccagggtg gggaccgaag
     1261 gcagcctggg cgaggtgacg agcaggacct ccgccccatg cgccgccagc gtgcgaccgc
     1321 agaccggccc ggcgatgatg cgtgtcaggt cgagcacgcg cacgccactg agcggccggg
     1381 cgccgtctgc cagcgacggc agcggctggg atggcgcgtc accgcccggt atacgctcga
     1441 tgctcaccag ggggagaccg gccaaggcct ggccttgcgg gctcgcatcc cattcctcga
     1501 agctgcgcat cgccgcaacg accatcttgg cctgggctgc cgcttcctcg aagcgctcgg
     1561 cctcccagcc gagcaaggcc tgggccaccg cggcacggtc gtagtcgcag cccagcaggc
     1621 gcagcacgcc gtcgcgatga tgcgggaagt tggtgtgcag cctcacccag cggccgtcgc
     1681 cgcagcgata ggcgcctgca atcctgtccc agggatccgg cggcgcggca ccatcgatgc
     1741 gcaaatagcg ctcgctgcgg aactcggccg ccgcgtgctg catgtccacg cgcacctgtt
     1801 gcgccggccc gccgcgcaag cgtcccagct cgcgcgctgc cagggccgcc gccgcgatgc
     1861 tcgcctgcgc cgctgtgccc accgcgaagc tcgacggcag ggccggctcg gcgccgctca
     1921 gttcgacagc ggccaaggcc tcgggcgcgc cgcccacgcc cagccaaagc tcgctgagca
     1981 catccttggg agccagagca tccatggttt tccttcgctg gcgtcaggaa cgccggct
//
LOCUS       DASUCY010000391         2165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_956410, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000391 DASUCY010000000
VERSION     DASUCY010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956410"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..1047)
                     /locus_tag="VFV25_07845"
     CDS             complement(88..1047)
                     /locus_tag="VFV25_07845"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY1393274.1"
                     /translation="MRNSIKISAITVAMAAMLAGCSRPYPKDEYLEIGPSETAFLIAL
                     DGDTRASQAKLSSIAYLEENKVQAKRIKIPHRIVDLCPSAPINHCWQDQPMAKLIKVS
                     RTPVTREWTKDPKTGTSARDEAFHVESNEAIDFTIGASLTAHVSEEGTAKFLFHYAGK
                     QLEEVMDTNMRAFVSSALSREFGSRSVMEARGQKNAIFAKVFQETKDAFEPRGLSLDA
                     FGYAEGMTFSDPKVQEAINRAFEAQANKATAMAQAEAAESFARAKDAINSMSDLEMRK
                     KLVDAQVEMLKKWDGRAPQVVTQDSMFGAMFATGRLAGVSSGR"
     gene            complement(1110..1370)
                     /locus_tag="VFV25_07850"
     CDS             complement(1110..1370)
                     /locus_tag="VFV25_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393275.1"
                     /translation="MRVVLSVVWSVLVLGVYQLVTPLASMQSQQVAVQQMHNTNASTA
                     AAEWFGNGQVWLGVVCLLVWGLVLCAIWARRIAAALRRHLKN"
     gene            1775..>2165
                     /locus_tag="VFV25_07855"
     CDS             1775..>2165
                     /locus_tag="VFV25_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393276.1"
                     /translation="MSALSPTADAASAARDERSDRAFFGHPAGLGWLAFCELWERFSY
                     YGMQALLVLYLSNYLFLPQNIGQVAGIDTLRGLIESAVGPRSPQQLASAVFGLYAGCV
                     YLTPLFGGLVADRLLGRTKTVVIGASLM"
BASE COUNT          336 a          724 c          681 g          424 t
ORIGIN      
        1 ttcagcagct agctgccgag cccacagcag gccgccgacc cgcatcgcct gcgcaggctg
       61 gcggccctcc gactcagtgg gctgggctca tcggccgctc gatacgcccg ccagcctgcc
      121 ggtcgcgaac atcgcgccga acatcgagtc ctgcgtcacg acttgcggcg cgcggccatc
      181 ccatttcttc agcatctcta cctgggcatc gaccagcttc ttgcgcatct ccaggtcgga
      241 catcgagttg atggcgtcct tggcccgcgc gaagctttcg gcggcctcgg cctgcgccat
      301 cgccgtggcc ttgttggcct gggcctcgaa ggcgcggttg atggcctctt gcaccttggg
      361 atccgagaag gtcatgcctt ctgcatagcc gaaggcatcc aggctcagcc cacgcggctc
      421 gaacgcgtcc ttggtctcct ggaagacctt ggcgaagatc gcgttcttct ggccgcgcgc
      481 ctccatcacg gagcggctgc cgaactcgcg cgacagcgcg ctgctgacga aggcgcgcat
      541 attggtgtcc atcacctctt cgagctgctt gccggcgtag tggaacagga acttcgcagt
      601 gccttcttcc gacacatgcg ccgtgaggct cgcgcctatc gtgaagtcga tggcctcgtt
      661 gctctccacg tggaaggcct cgtccctggc cgaggtgccg gtcttcggat ccttggtcca
      721 ctcgcgggtc accggcgtgc gcgacacctt gatcagcttg gccatgggct ggtcctgcca
      781 gcagtgattg atcggtgccg acgggcacag gtccacgatg cgatgcggga tcttgatgcg
      841 cttggcctgg accttgttct cctccagata ggcgatcgac gacagcttgg cctggctcgc
      901 ccgggtatca ccgtcgagcg cgatcaggaa ggcggtctcg gaagggccga tctccagata
      961 ctcgtccttc ggatagggcc ggctgcagcc ggccagcatg gccgccatgg ccacagtgat
     1021 ggcggaaatc ttgatgctgt ttctcatgat ctcttccttc cgcggctggg tgccatgcac
     1081 cgagccgcat tcattggggg ttctggtctt cagttcttga gatggcggcg cagcgccgca
     1141 gcgatgcggc gggcccagat cgcgcagagg accaggcccc acaccagcag gcagacgacg
     1201 ccgagccaga cctggccatt gccgaaccat tcggccgcgg cggtggacgc gttcgtgttg
     1261 tgcatttgct gcacggcgac ttgttgcgat tgcatcgagg ccagcggcgt caccagctga
     1321 taaacgccaa ggaccaggac cgaccagacg acagacaaaa cgactctcat tgccgactcc
     1381 aagagggcaa gctttgaagc gcgcacgagt tcgcgcaaag ccgagcccga aggctcaggc
     1441 gaacatgcgc atctggcgct ggctgcggga tgctgcggcc agcgcggagc gtgagaggca
     1501 gtgtgattta ggggatgcgc gaacgctgcc gcttggggct gcgcatcgtc ttgcaaagcg
     1561 ctcccaaggc caccggcagg gcttgccggg cgagtgctgc gacatcgatg tcgatacgca
     1621 tggggagcct tcgattcttt ttttcagcgc attgctgcgc cgcaacatca atatacgctt
     1681 agcccggaga tttgcaagta gcggcggcgg gagttgtccc ctctgcccat ttcgacgaca
     1741 gtcgcggctt ctccgcttcc tcgagccatc caaagtgtcc gctctctccc ccaccgcaga
     1801 cgccgcgtca gccgctcgcg acgagcgctc cgatcgcgcc ttcttcggcc atccggccgg
     1861 cctgggctgg ctcgccttct gcgagctctg ggaacggttc tcctactacg gcatgcaggc
     1921 gctgctggtg ctgtacctga gcaactacct cttcctgccg cagaacatcg gccaggtcgc
     1981 cggcatcgac accctgcgcg gcctcatcga gtccgccgtc gggccgcgct cgccgcagca
     2041 gttggcctct gccgtcttcg gcttgtacgc cggctgcgtc tacctcaccc ccttgttcgg
     2101 cgggctggtg gcggatcgcc tgctcggccg cacgaagacg gtcgtcatcg gcgcgagcct
     2161 gatgg
//
LOCUS       DASUCY010000392         2205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_137244, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000392 DASUCY010000000
VERSION     DASUCY010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137244"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="VFV25_07860"
     CDS             <1..646
                     /locus_tag="VFV25_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016453036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEY1393277.1"
                     /translation="AARNHEAADLYQRVQNVARGAALMTGCEVDIRFDKACSNLLQNT
                     ALNQVLYEEMQRLGPLPVSDSEREQAQRDHATLSENDVAEASRSLGSVLRGQVPALFD
                     GVKDYDPQRVELLYGSTDVADVSWITPTAQCWVACYAFGTPFHSWQMVSQGKSSVAHI
                     GMVRAAKAMAATAIRLLEQPQVLAVAREELQERRAGKPYVCPIPTEVPLPFQRA"
     gene            complement(782..2158)
                     /locus_tag="VFV25_07865"
     CDS             complement(782..2158)
                     /locus_tag="VFV25_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393278.1"
                     /translation="MAKFFKWLVLLALALLGLLAAVAYMLHRWVGTDDFRQRVESEAA
                     AALGVPVALGGVAVEVWPLPAVALSGIEVQSKPPLTLGRVEVRPQWRPLLQGRLAVAT
                     LLVRRAVLPQQGIDAVLLLLQKKKQTAPVTPGPQPKNPANFAPEAAHLEWLPRRTVLD
                     DVTWISAGGARTTLQAEAKLGADGLPDGVSLQLLKGHLQGLRATLEREASEAHAASGG
                     GQWALRVDVGGGKVEGRLGLQRAPAAASARAGEAPLSVLLMNGQLQTHGVEVSALTAP
                     SRALTGQLEASTTLQARAATTAALGEALQSRTTFTVRNAVLNGLDLAKAVQTVGLSRG
                     GQTRLDTLAGQVSTQGRAAQLSNLVASSGVLSATGNVAISPAKALSGRVTVAVAGDSK
                     IGSAIGGAVGVPLTVGGTLDNPEVTLSRSALLGAAIGTALLPGVGTGAGANLGDRMGE
                     GLRGLFGK"
BASE COUNT          334 a          828 c          724 g          319 t
ORIGIN      
        1 cgccgcgcgc aaccacgagg ccgccgatct ttaccagcga gtgcagaacg tggcgcgtgg
       61 cgccgcgctg atgaccggct gcgaggtcga tatccgtttc gacaaggcct gctccaatct
      121 gctgcaaaac acggcgctca accaggtgct atatgaagaa atgcagcggc taggccccct
      181 gccggtgagc gacagcgagc gcgagcaggc ccagcgcgac catgccacgc tgagcgagaa
      241 cgacgtggcc gaggccagcc gctcgctggg cagcgtactg cgcggccagg tgccggccct
      301 gttcgatggc gtgaaggact acgaccccca gcgcgtggaa ttgctgtacg gctcgaccga
      361 tgtggccgac gtcagctgga tcacgcccac cgcccaatgc tgggtcgcct gctatgcctt
      421 cggcacgccc tttcactcct ggcaaatggt gtcgcagggc aagtcgtcgg tggcgcatat
      481 cggcatggtg cgggccgcca aggccatggc cgcgaccgcc atccggctgc tcgaacagcc
      541 acaagtgctg gcggtggccc gcgaggagtt gcaggagcga cgcgccggca agccctatgt
      601 gtgcccgatt ccgaccgagg tgccgctgcc ttttcagcgc gcctgacccc gccgcgcggt
      661 agcgcaaccc tgcggaacgg cgcggtatcg ccgccaccat gagctctgtt tttgatagct
      721 gtcagcgccc aactcgcacg ggatggccgc tcaattggcc actatcccag gcctgcgggc
      781 gctacttgcc gaacaggccg cgcaagccct cgcccatgcg gtcacccagg ttggcgccgg
      841 cgccggttcc cacgcccggc agcagggccg tgccaatcgc cgcacccagc aaggccgaac
      901 gcgacagcgt gacctcgggg ttgtccagcg tgccacctac cgtgagcggc acaccgacgg
      961 caccgcctat ggcgctgccg atcttgctgt cgcccgccac cgccacggtg acccgtccgc
     1021 tcagcgcctt ggccggcgag atcgcgacgt tgccggtggc gctcaggacg cccgagctgg
     1081 ccacgaggtt gctcagctgg gccgcccggc cctgcgtgct gacctgcccg gccagcgtgt
     1141 ccaggcgggt ctggccgccg cggctcaggc ccacggtttg caccgccttg gccaggtcca
     1201 gaccgttgag caccgcattg cgcacggtaa aggtggtgcg gctttgcagc gcctccccca
     1261 gcgccgccgt ggtggcggcc cgggcctgca gcgtggtcga ggcctccagc tggccggtga
     1321 gcgcccggct gggcgcggtc agcgccgaga cctcaacacc gtgcgtttgc aactggccgt
     1381 tcatcaacag gacggacagc ggcgcttcgc cggcccgggc cgacgcggcc gcgggcgccc
     1441 gctgcaggcc cagccggccc tcgaccttgc cgccccccac gtcgacgcgc agcgcccact
     1501 ggccgccgcc tgacgcggca tgggcctcgg acgcctcgcg ctcaagcgtg gcccgcagcc
     1561 cctgcaggtg gcccttgagc agttggagcg agacgccgtc cggcaggccg tcggcgccca
     1621 gtttcgcctc ggcctgcagc gtggtgcgcg cgccgccggc gctgatccag gtcacatcgt
     1681 ccagcacggt gcggcgcggc aaccattcca ggtgggccgc ctccggcgcg aaatttgcag
     1741 gattttttgg ctgcggccca ggcgtgacgg gcgctgtctg cttctttttt tgtagcaaca
     1801 acagaacagc gtctatgccc tgctgcggca acaccgcgcg gcgcaccagc agcgtggcca
     1861 ccgccagccg cccctgcagc aacggccgcc actgcggccg cacttccacg cgccccaggg
     1921 tcaagggggg cttgctctgc acctcgatgc cgctcaacgc cacggccggc aagggccaca
     1981 cctccacggc gacgccgccc agcgccaccg gcacgccgag cgccgccgcc gcctcgctct
     2041 cgacgcgctg cctgaagtcg tccgtgccga cccagcggtg cagcatgtac gccaccgcgg
     2101 ccagcaaccc caacagcgcc agcgccagca acaccagcca tttgaagaat tttgccatcc
     2161 ggctattgtg gcggctgcaa ggccgcgcgc tgcagaaaat ccgcc
//
LOCUS       DASUCY010000393         2625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1068949, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000393 DASUCY010000000
VERSION     DASUCY010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1068949"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VFV25_07870"
     CDS             <1..460
                     /locus_tag="VFV25_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY1393279.1"
                     /translation="HLGIQGGSNGGLLVGAVAMQRPELFRAVVCQVPLLDMRRYNKLL
                     AGASWMAEYGNPDQPEEWAYISRYSPYQNVRREAQYPTMLLTTSTRDDRVHPGHARKM
                     VARMMEQGHPVLYYENIEGGHGGAADNAQRAYLQALEFTYLWERLGGATP"
     gene            626..1276
                     /locus_tag="VFV25_07875"
     CDS             626..1276
                     /locus_tag="VFV25_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY1393280.1"
                     /translation="MARSYTDRSSPWRLVVLGTVAVVHGLLAYALATGLGRKAFDVIK
                     KPVMARILEEPPPPPPPPPPPPPKPVEQLAKPRAPVAPPPPYVPPPEVVPPAAAGPAI
                     TAVTTTPPVQLPVIAPPPPPAPPAPPPVAKAPVSIGVVCPTQVEPKMPPKAIAEDLSG
                     VVRARMIISGGVVKQVTILSGPREFHAAVRAAVLQYKCLSGEDNVVVEQEFDFKLQ"
     gene            1338..2231
                     /locus_tag="VFV25_07880"
     CDS             1338..2231
                     /locus_tag="VFV25_07880"
                     /inference="COORDINATES: protein motif:HMM:NF013788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C13 family peptidase"
                     /protein_id="HEY1393281.1"
                     /translation="MPSLFSRMRHAALTLLLASFCCCGLLAGAQAMAASSPLQAEYAQ
                     AQRAANQAGEQRLVLVAAAMDASSQAFVGDVKLVERELRRFAPGLVALHLANVPGGPQ
                     RKAWPEATPRALQLTLARAGTLLHGGKPEESLAVVLLSSHGHVGLLSAARNARDYSDV
                     HVSELREWLQPLGETPTLLIISSCYSGSLIPLLEQDNRIIITAASDRTSSFGCEPESH
                     NTYFIDSLFGDGLDPKESLEQWFARGAKLVAQREAQMQLTPPSNPQLWVGKAMKRLAN
                     RPLRELLGSFGAGSPSYNSRP"
     gene            2228..>2625
                     /gene="pyrF"
                     /locus_tag="VFV25_07885"
     CDS             2228..>2625
                     /gene="pyrF"
                     /locus_tag="VFV25_07885"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEY1393282.1"
                     /translation="MSFLARLQQAARLNDSLLCVGLDPEPARFPAPWKGDAGRIYDFC
                     ARIVDATKDLVAAFKPQIAYFAAHRAEDQLERLMAHLRRVAPDVPVILDAKRGDIGAT
                     AEQYAREAYERYQADAVTLSPFMGFDSIEP"
BASE COUNT          416 a          961 c          852 g          396 t
ORIGIN      
        1 gcacctgggc atccagggcg gcagcaatgg cggcctgctg gtcggcgccg tggccatgca
       61 gcgccccgag ctcttcaggg ccgtggtctg ccaggtgccg ctgctcgaca tgcgccgcta
      121 caacaagctg ctggccggcg cctcgtggat ggccgagtac ggcaaccccg accagcccga
      181 ggaatgggcc tatatctcgc gctacagccc ctaccagaac gtcaggaggg aggcccagta
      241 cccgaccatg ctcctcacca cctcgacgcg cgacgaccgc gttcaccccg gccatgcgcg
      301 caagatggtc gcgcggatga tggaacaggg ccacccggtg ctctactacg agaacatcga
      361 aggcggccac ggcggcgcgg ccgacaacgc ccagcgcgcc tatctgcagg cgctggagtt
      421 cacctatctc tgggagcggc tgggcggtgc gacgccgtag atgaccgcac ccggtcgcgg
      481 actacctgta agcaagccca tacacccgag ccggggatga aacgcagggc ccgctgcgtg
      541 acaatcgacg cgtccacgcc ctgaaggtcc aagcggcggc cggccagcaa aaggcaaact
      601 gccgctggtc ccatgtctat acgtcatggc ccgcagctat acggacagga gttccccctg
      661 gcgcctcgtc gtgctgggga cggtcgcggt ggtgcacggc ctgctggcct atgcactggc
      721 gaccgggctg ggccgtaagg cgttcgacgt gatcaagaag ccggtcatgg cgcgcatcct
      781 cgaagagccg ccaccgccgc cgcctcctcc accgccgcca ccgcccaagc ctgttgagca
      841 gctggcaaag ccgcgcgcgc cggtggctcc gcccccgccc tatgtgccgc cgcccgaagt
      901 agtgccgccc gctgcggccg ggccggccat cacggcggtg acgaccacgc cgcccgtgca
      961 gctgccggtc atcgcgccgc ctccgccgcc cgcaccgccg gcgccgccgc cggtggccaa
     1021 ggcgccggtc agcatcggcg tggtctgccc cacccaggtg gagcccaaga tgccgcccaa
     1081 ggcgatcgca gaggacctga gcggcgtcgt ccgcgcgcgc atgatcatca gcggcggcgt
     1141 ggtcaagcag gtcacgatac tctcgggtcc gcgcgagttt cacgccgcag tgagggccgc
     1201 ggtgctgcaa tacaaatgcc tgtcgggcga ggacaacgtc gtggtcgagc aggagttcga
     1261 cttcaagctc caatagcctt gcggcggccg caggcccgcc atcctcatcc tctgtttcat
     1321 tgtttgctca tcctcacatg cccagcctct tcagccgcat gcgccatgcg gcgctgaccc
     1381 tgctcctggc ctctttttgc tgctgcggcc tgctcgccgg cgcgcaggcc atggccgcaa
     1441 gcagcccgct gcaagccgag tacgcgcagg cgcagcgcgc cgccaaccag gccggcgagc
     1501 agcgcctcgt gctggtcgcg gcggcgatgg atgccagctc gcaggccttc gtcggcgacg
     1561 tcaagctggt cgagcgtgag ctgcgccgct tcgcgccggg gctggtcgcg ctgcatctgg
     1621 cgaatgtgcc cgggggcccg cagcgcaagg cctggccgga agcgacgccg cgcgccctgc
     1681 agctcacgct ggcgcgcgcc ggtaccctgc tacacggcgg gaagcccgaa gaatccctgg
     1741 ccgtggtgct actcagctcg catggccatg tgggcctgct ctcggccgcc cgcaatgcgc
     1801 gcgactacag cgatgtgcat gtgagcgagc tgcgcgagtg gctgcagccg ctgggcgaga
     1861 cgccgaccct gctgatcatc tcgtcctgct actcgggctc gctgatcccg ctgctggagc
     1921 aggacaaccg catcatcatc accgccgctt cagaccgcac gagctccttc ggctgcgagc
     1981 ccgagagcca caacacctat ttcatcgact cgctgttcgg cgacggcctg gacccgaagg
     2041 agagcctcga gcaatggttc gcgcgcggcg ccaagctcgt cgcacagcgc gaagcgcaga
     2101 tgcagctgac gccgccttcc aatcctcaac tgtgggtggg caaggcgatg aagaggctgg
     2161 cgaaccgacc gctgagagag ctgctgggtt cattcggcgc cggctcgcct tcttacaatt
     2221 cgcgcccatg agcttcctcg cccgcctcca gcaagccgcc cgcctcaacg actccctgct
     2281 ctgcgtgggg ctcgaccccg agcccgccag gttccccgcg ccctggaagg gcgatgcggg
     2341 ccgcatctac gacttctgcg cgcgcatcgt cgatgcgacc aaagacctcg tcgccgcctt
     2401 caagccgcag atcgcctatt tcgcggcgca tcgtgccgag gaccagctgg agcggctgat
     2461 ggcgcacctg cgccgtgtcg cgcccgacgt gccggtgatc ctggacgcca agcgcggcga
     2521 catcggcgcc accgccgagc aatatgcgcg cgaggcctac gagcgctatc aggccgatgc
     2581 ggtgacgctc tcgcccttca tgggcttcga ctcgatcgag cccta
//
LOCUS       DASUCY010000394         2075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_185767, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000394 DASUCY010000000
VERSION     DASUCY010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185767"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..756
                     /locus_tag="VFV25_07890"
     CDS             28..756
                     /locus_tag="VFV25_07890"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HEY1393283.1"
                     /translation="MEVTWARDEGEVREAQRLRHLVFAEEMGARLSPPAGAPAGHDID
                     VFDPYCEHLLVRAFGHAGEPGMVIGTYRVLTPEAARRIGGLYSETEFDLTRLRPLRGK
                     MVELGRSCVHPEHRSGGAILALWGALAEFMVRNELDTMVGCASISMRDGGHMAASLWE
                     QLRHTHLAPIELQVRPRLPLPVEELQSDLTVEAPALIKGYLRCGAKVLGPPAWDPDFN
                     TADLPMLMRIQDLPAKYRKHFLGA"
     gene            complement(840..1109)
                     /locus_tag="VFV25_07895"
     CDS             complement(840..1109)
                     /locus_tag="VFV25_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEY1393284.1"
                     /translation="MIKSTISISNKLGLHARASAKLTKLAGGYPCDVHMSRNGRRVNA
                     KSIMGVMMLAAGLGTEVEIETDGDQEHQAMEAIRRLIDDKFGEGE"
     gene            complement(1078..1557)
                     /locus_tag="VFV25_07900"
     CDS             complement(1078..1557)
                     /locus_tag="VFV25_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEY1393285.1"
                     /translation="MAGIFIIAHAPLASALLAAASHAFPEAAAEVLALDVQAELPLAE
                     TEAQARGMLERAIARACEASGGQQAEVLILTDVFGATPANLAQRLAEGGNVKVVTGVN
                     VPMLWRSLNYAQEPLDTLVARALAGGAQGVMQVATSRPQNQAYKPSANDQEHHQHQQ"
     gene            complement(1696..>2075)
                     /locus_tag="VFV25_07905"
     CDS             complement(1696..>2075)
                     /locus_tag="VFV25_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393286.1"
                     /translation="QMRLHAGEAQGASWALSHADVGDPARVGPALQELRAALLQHLGG
                     REASAPPMPISVPGMTPQAEAQRFTLRGERGGEGKAMELQVGLFSRGSVVFQAVVMKP
                     AAAREPDAEAVDSFFNGLKLGSP"
BASE COUNT          309 a          725 c          707 g          334 t
ORIGIN      
        1 gagcagcctg ttcgcgagcg cgttcgactg gaggtcactt gggcgcgcga tgaaggcgaa
       61 gttcgcgaag cgcagcgcct gaggcacctg gttttcgccg aggagatggg tgcccgcctg
      121 tctccgccgg ctggcgcccc agccggtcat gacatcgacg tcttcgaccc ttattgcgag
      181 catctgctgg tgcgcgcctt tggccacgcc ggcgagcccg gcatggtgat cggcacctac
      241 cgcgtgctga cccccgaggc ggcccgacgc atcggcgggc tgtacagcga gaccgaattc
      301 gacctgaccc gactgcgccc gctgcgcggc aagatggtcg agctgggccg ctcctgcgtg
      361 catccggaac accgctcggg cggcgccatc ctggcgctgt ggggcgcgct ggccgagttc
      421 atggtgcgca acgagctgga caccatggtc ggctgcgcca gcatcagcat gcgcgacggc
      481 ggccatatgg cggccagcct gtgggaacag ctgcgccaca cgcatctggc gccgatcgag
      541 ctgcaagtgc gcccgcgcct gccgctgccg gtggaagagc tgcaaagcga cctgacggtc
      601 gaggcgcccg cgctcatcaa gggctatctg cgctgcggcg ccaaggtgct gggcccgccg
      661 gcctgggacc ccgacttcaa cacggccgac ctgccgatgc tgatgcgcat ccaggacctg
      721 ccggccaagt accgcaagca cttcctcggt gcatgaggct tcggccacca ggacaaagag
      781 aaaaccaccg cctgccggtg gttttctgcc atttggcagt ccgaaaaccg tagcgccgcc
      841 tactcgcctt cgccgaactt gtcgtcgatc agcctgcgga tggcctccat cgcctggtgc
      901 tcctggtcgc cgtcggtctc gatctcgacc tcggtgccca ggccggcggc cagcatcatc
      961 acgcccatga tgctcttggc attgacgcgc cggccgttgc ggctcatgtg gacgtcgcag
     1021 gggtagccgc cggcaagctt ggtgagtttg gccgaggccc gcgcatgcag gcccagctta
     1081 ttgctgatgc tgatggtgct cttgatcatt ggcagacggt ttgtaggctt ggttctgcgg
     1141 acgggaggtg gcgacctgca tcacgccctg ggcgccgccg gccagggcgc gcgccaccaa
     1201 cgtgtccagc ggctcctggg cgtagttgag gctgcgccac agcatgggca cgttgacccc
     1261 ggtgacgacc ttcacgttgc cgccctcggc caggcgctgg gccagattgg cgggcgtggc
     1321 gccgaagaca tcggtgagga tcagcacctc tgcctgctga ccgcccgagg cctcgcacgc
     1381 gcgggcgatg gcacgctcca gcatgccgcg cgcctgggct tcggtttccg ccagcggcag
     1441 ctctgcttgc acgtcgagcg cgaggacctc ggccgccgcc tcggggaagg catggcttgc
     1501 cgcggccagc agggccgagg ccagcggggc gtgggcgatg atgaagatgc cggccatgtt
     1561 cgcttcgatt atcggcggag ccgtggttca gccccgtgac agattccgcc ttgcgggtgg
     1621 atgcgcgtgg ccgacccaaa agcctggcac acgccgctga caacgcggca ggccgcgtcc
     1681 gcgccgacat gggcttcagg gagagccaag tttcaagccg ttaaaaaagc tgtccaccgc
     1741 ctcggcgtca ggctcgcggg ccgccgccgg cttcatcacc acggcctgga agacgaccga
     1801 gccccggctg aacagcccga cctgcaactc catcgccttg ccttcgccgc cgcgctcgcc
     1861 gcgcagcgtg aagcgctggg cctcggcctg cggcgtcatg ccgggcacgg aaatgggcat
     1921 gggcggcgca ctcgcttcgc gcccgcccaa gtgttgcagc aaggccgcgc gcagctcctg
     1981 cagcgccggc ccgacgcgcg ccgggtcgcc cacatcggca tggctcaagg cccagcttgc
     2041 gccctgcgcc tcaccggcat ggagccgcat ctgca
//
LOCUS       DASUCY010000395         2953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_298130, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000395 DASUCY010000000
VERSION     DASUCY010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298130"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..793)
                     /locus_tag="VFV25_07910"
     CDS             complement(38..793)
                     /locus_tag="VFV25_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY1393287.1"
                     /translation="MLDLRWDILSGYGPLFVAGLWTTIELTLVAIGAGLLLGVLLGLV
                     SSSKDAPRPRSRALAWGLVLLRGVTRGYVAFFRGTPLFVQILLVHFALMPTLIHPEHG
                     LLLTGEAAREFRQAHGAFFSGALAMTLNAGAYISEIFRAGIQSIHLGQTQAAYSLGLN
                     HTQAMRHVILPQAFRRMVPALVNEGVTLIKDSSLVSAIGLAELALAARTVAGAYSRYW
                     EPYLAISAIYLVLTLLLSTVAKRLEAPAHLRGR"
     gene            complement(804..1661)
                     /locus_tag="VFV25_07915"
     CDS             complement(804..1661)
                     /locus_tag="VFV25_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY1393288.1"
                     /translation="MTTPTLRQFLKMSGLLLAGLVLAACSKQGGGESGGAASAPTAAA
                     PAAAPNKVYVVGTDAAYAPFESQNEKGEIVGFDIDVVNAVAKKAGIEVKFVNTPWEGI
                     FNNLQQGDRDLLVSAITITDERKQTVDFSEPYFDAQQLIAVKQGSKVAKFADLKPLKV
                     GVQNGTTGDEVVSKLLGKTNTNIKRFESTPLALKELEAGGVDAVVADNGVVVNYVANN
                     AAAGFKTVSDPSFAQEHYGIAVRQGDAELKGKIDKALAAIKADGSYAQIYAKYFGAQP
                     IAAAAAASK"
     gene            complement(1809..2609)
                     /locus_tag="VFV25_07920"
     CDS             complement(1809..2609)
                     /locus_tag="VFV25_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEY1393289.1"
                     /translation="MDLRRSCLQALALADVSEKLAAVAALWDERRDALSIDADAPLAS
                     QPDLPALPGRPPLPRLVDPGSVPQRSPFTTEGRAALLHAIAHIEFNAINLALDAVWRF
                     AGMPSDYYRDWLRVAKEEAYHFGLLREHLRSLGRDYGDFDAHDGLWAMAEKTRDDIVA
                     RMALVPRTLEARGLDASPAIQAKLAKAGDARAVEILAIILRDEVGHVAIGNHWYRWLC
                     EREGLDPVAHYSALTRRHEAPRLKPPFNDEARLAAGFTEEELAWLRGV"
     gene            2672..>2953
                     /locus_tag="VFV25_07925"
     CDS             2672..>2953
                     /locus_tag="VFV25_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEY1393290.1"
                     /translation="MAVYQLGDDVPQIHETAWVADSAQVIGRVELAENASVWFGTVVR
                     GDSERLTIGRNSNVQDGSVLHADAGVPLVVGENVTVGHKVMLHGCQIGDG"
BASE COUNT          468 a          962 c         1025 g          498 t
ORIGIN      
        1 tcgggggtgt ccagcgttcc cgctggacga ggggccgtca gcgtccgcgc aggtgtgccg
       61 gcgcttccag ccgtttcgcc accgtggaca gcagcagcgt cagcaccaaa tagattgccg
      121 agatcgcgag atagggttcc cagtagcgcg aataggcgcc ggccacggtg cgcgcggcca
      181 gggccagctc ggccaggccg atggccgaga ccagcgatga atccttgatc agcgtgacgc
      241 cctcgttgac cagggccggc accatgcgcc tgaaggcctg gggcaggatc acgtggcgca
      301 tcgcctgggt gtggttgagg cccaggctgt aggcggcctg ggtctggccg aggtggatgc
      361 tctggatgcc ggcgcggaag atctcggaga tataggcgcc ggcattgagt gtcatcgcca
      421 gcgcgcccga gaagaaggcg ccatgcgcct ggcggaactc gcgcgccgcc tcaccggtga
      481 gcagcagccc atgctcgggg tggatcagcg tgggcatcag cgcgaaatgc acgagcagga
      541 tctgcacgaa gagcggcgtg ccgcgaaaga aggcgacata gccgcgcgtc acgccgcgca
      601 gcagcacgag gccccaggcc agcgcccgcg acctgggccg cggcgcatcc ttggaactgc
      661 tcaccagccc cagcagcacg cccagcagca ggccggcgcc gatggccacc agcgtgagct
      721 ctatcgtcgt ccacagcccg gcaacgaaca gcggcccata gccggagagg atatcccagc
      781 gcaaatcgag catgtcgctt aggctatttc gacgccgccg cagccgcggc gatcggctgg
      841 gcgccgaaat acttggcgta gatctgcgcg tagctgccgt cggccttgat cgcagccaag
      901 gccttgtcga tcttgccctt cagctcggcg tcgccctggc gcactgcgat gccgtagtgc
      961 tcctgcgcga agctcgggtc gctcacggtc ttgaagccgg cggcggcgtt gttggcgaca
     1021 tagttgacga cgacgccgtt gtcggccacc acggcatcga cgccgcccgc ttccagctcc
     1081 ttgagtgcca gcggcgtgga ctcgaagcgc ttgatattcg tattggtctt gccgagcagc
     1141 ttggagacga cctcgtcgcc cgtggtgccg ttctgcacgc cgaccttcag cggcttcagg
     1201 tctgcgaact tcgcgacctt ggagccctgc ttgacggcaa tcaactgctg ggcgtcgaaa
     1261 tagggttcgc tgaaatccac ggtctgcttg cgctcgtcgg tgatcgtgat ggccgagacc
     1321 agcaggtcgc ggtcgccctg ctgcaggttg ttgaagatgc cttcccaggg cgtgttgacg
     1381 aacttgacct cgatgccggc cttcttcgcc accgcgttca cgacgtcgat gtcgaagccg
     1441 acgatctcgc ccttctcgtt ctgcgactcg aagggcgcat aggcggcgtc ggtccccacc
     1501 acatagacct tgtttggcgc cgctgcgggc gcggcggccg tgggcgcgga agccgcaccg
     1561 ccgctctcgc cgccgccctg cttgctgcag gccgccagca cgaggcctgc caggagcaag
     1621 ccgctcatct tcaaaaactg gcgcagggtc ggtgtcgtca tggtgcttgc tcttttcttt
     1681 ccttggaaga ttttgcgagg cgtgcgcagt ttagagcctg gcgcagggcc ggcttatcgg
     1741 ggttggcagg gggttggggt gtcaggcaga tgtgcaagat tcgggccctg cgcggggctt
     1801 cttcggcatt acacgccgcg cagccaggcc agctcttcct cggtgaagcc ggcggccaga
     1861 cgggcctcgt cgttgaaagg cggcttgagc cgcggggcct cgtggcgccg tgtcagcgcg
     1921 ctgtaatgcg cgaccggatc cagcccctcg cgctcgcaca gccagcgata ccaatggttg
     1981 ccgatcgcca catggcccac ctcgtcgcgc aggatgatgg ccaggatttc cacagcccgc
     2041 gcatcgcccg ccttggccag cttggcctgg atggccggcg aggcatccag gccgcgcgcc
     2101 tccagcgttc gcggcaccag ggccatgcgg gcgacgatgt cgtcgcgcgt cttctcggcc
     2161 atggcccaca ggccatcgtg cgcatcgaag tcgccgtagt cgcggcccag gctgcgcagg
     2221 tgctcgcgca gcaggccgaa gtgataggcc tcctccttgg ccacgcgcag ccagtcgcgg
     2281 tagtagtccg acggcatgcc cgcgaagcgc cagaccgcgt ccagcgccag gttgatcgcg
     2341 ttgaattcga tgtgcgcaat cgcatgcagc agcgcggcgc ggccctcggt ggtgaagggc
     2401 gagcgctggg gcaccgagcc cgggtccacc agccgcggca agggtgggcg gccgggcagg
     2461 gcgggcaaat cgggctggct tgcgagcgga gcatcggcat cgatcgaaag cgcgtcgcgc
     2521 cgctcatccc atagggcggc cacggcggcc agcttctcgc tcacgtcggc cagggccaag
     2581 gcctgcaggc aactgcgtct gaggtccatc cttagaattc taagtttcgg gtgcctggcg
     2641 cctgtccgtc caaagtttct gcgaggtgag cgtggcggtc tatcagctcg gggacgacgt
     2701 gccccagatc cacgaaacgg cctgggtggc cgacagcgcc caggtgatcg gccgcgtcga
     2761 gctggccgag aatgccagcg tgtggttcgg caccgtggtg cgcggcgaca gtgagcggct
     2821 gaccataggc cgcaacagca acgtgcagga cggctcggtg ctgcatgccg atgccggcgt
     2881 tccgctggtc gtcggcgaga acgtcacggt gggccacaag gtgatgctgc acggttgcca
     2941 gatcggcgac ggc
//
LOCUS       DASUCY010000396         3124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_474691, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000396 DASUCY010000000
VERSION     DASUCY010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474691"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..142)
                     /locus_tag="VFV25_07930"
     CDS             complement(<1..142)
                     /locus_tag="VFV25_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY1393291.1"
                     /translation="MKRRELIGSIAAAGAGLALPALAQGERSIVLGQSAAFTGPAAQL
                     GIQ"
     gene            299..2338
                     /locus_tag="VFV25_07935"
     CDS             299..2338
                     /locus_tag="VFV25_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HEY1393292.1"
                     /translation="MSISIVRAACPHDCPDTCAMRVTVEDGRVVRVQGDPDHPTTHGA
                     LCTKVSRYAERSYHPDRVLRPLKRSGPKGSGQFTPVSWDEALADIAARLQAIAARDPE
                     AILPYSYAGTMGLVQGESMAARFFHKLGASLLDRTICSSAGGEALAASYGGKVGMHLE
                     HFAESRLILIWGSNSITSNLHFWTYAQAAKRNGAKLICIDPRRTETADKCHQHIALLP
                     GTDGALALGLMRELIVNDWLDHDYIAAHVEQGPQGWEALRDKAMAWTPERTAATCGIS
                     ADEVRQLARDYGTLKPAAIRVNYGMQRVRGGGNAVRLIATLPCLVGAWRHRAGGLLLS
                     SSGWFPVNKAGLQRPDLLAGRQPRTINMSTIGDDLLRETSPAFGPKIAALVVYNSNPV
                     AVAPESPKVVQGFQREDLFTVVLEHFMTDTADHADYVLPATTQLEHLDVHSSYGHTYA
                     LINEPAIAPLGEALPNSEIFRRLAARMGYTEPCFGESDEQIAAQAFGDHIDFAQLRRA
                     GWVKLPLPEAPFANGGFPVPGGRCRINAPGLGVPDYVPPYESAASAPELAQRYPLAMI
                     SPPARHFLNSTFVNVKSLRSVEGQPLVEIHPSDAAARGIADGAMVRVYNDRGAYRCVA
                     ELSERARPGVVNGLGIWWRKLGPDGTNVNEVTHQRLTDIGRAPSFYDCLVEVAAL"
     gene            2392..>3124
                     /locus_tag="VFV25_07940"
     CDS             2392..>3124
                     /locus_tag="VFV25_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEY1393293.1"
                     /translation="MAALAAATLCLTSGCSSFGYLAQSAHGQLSLMSAARPVSQVLAE
                     PDTSAELRARLELSQRMRDYAIRELKLPDNRSYRSYADLHRGAAVWNVVAAPELSLQL
                     KTWCFPIVGCVGYRGYFDRASADAAADELRHEGEEVSVYGVPAYSTLGWLNWLGGDPL
                     LNTFIRYPEGELARMIFHELAHQEAYAAGDTEFNESFATCVEQIGGRRWLSEQAGAAA
                     REEYERYDARRRDFQALTMAYRDKLD"
BASE COUNT          507 a         1088 c         1057 g          472 t
ORIGIN      
        1 tctgtatgcc cagctgggcg gccggtccgg tgaaggcggc cgactggccg agcacgatgc
       61 tgcgctcgcc ctgggccagg gcgggaaggg cgaggccggc accggcggcg gcaatgctgc
      121 cgatcagttc gcgtcgtttc atggctttcc caaagacaat ttgtttcaat cgcggacgaa
      181 gatgcgtatg cggcgctggg ccatgcctcg gcgactggag aatagtgcca gcgctatctc
      241 cgcagcaagt atcgggctct gccctctatt cttctgcagc gcgctatgct gatcgcatat
      301 gagcatctcc atcgtccgcg ccgcctgccc tcatgattgc cccgacacct gcgcgatgcg
      361 cgtcacggtg gaagacggcc gcgtcgtgcg cgtgcagggc gaccccgatc atccgaccac
      421 ccatggcgcg ctgtgcacca aggtgtcccg ctatgccgag cgcagctacc accccgaccg
      481 ggtgctgagg ccgctcaagc gcagcggacc caagggcagc gggcagttca cgccggtgag
      541 ctgggacgag gcgctggccg acatcgctgc gcggctgcag gcgatcgccg cacgcgatcc
      601 cgaggccatc ctcccctaca gctatgccgg caccatgggc ctggtgcagg gcgagagcat
      661 ggcggcgcgc ttcttccaca agctgggcgc ttcgctgctg gatcgcacca tctgctccag
      721 cgcaggaggc gaggcgctgg ccgccagcta tggcggcaag gtgggcatgc atctggagca
      781 tttcgccgag agccggctga tcctgatctg gggcagcaac tcgatcacgt ccaacctgca
      841 tttctggact tatgcccagg ccgccaagcg caacggcgcc aagttgatct gcatcgaccc
      901 gcggcgcacc gagacggccg acaagtgcca ccagcacatc gcgctgctgc cgggtaccga
      961 cggcgcgctg gcactgggcc taatgcgcga gctgatcgtc aacgattggc tggatcacga
     1021 ctacattgcc gcccacgtcg agcagggtcc gcagggctgg gaggccttgc gcgacaaggc
     1081 catggcctgg acgccggagc gcaccgccgc cacctgcggc atcagcgccg acgaggtgcg
     1141 acagctggcg cgcgactacg gcacgctcaa gcccgcggcg attcgtgtca actacggcat
     1201 gcagcgggtg cgcggcggcg gcaacgcggt gcggctgatc gcgacgctgc cctgcctggt
     1261 cggcgcctgg cggcatcgcg ccggcggtct gctgctgtcc agctcgggct ggttcccggt
     1321 caacaaggcg gggctgcagc ggcccgacct gctggccggg cgccagccgc gcaccatcaa
     1381 catgagcacg ataggcgacg atctgctgcg cgagacctcg cccgccttcg ggccgaagat
     1441 agcggcgctg gtcgtctaca acagcaaccc cgtcgcggtg gcaccggagt cgcccaaggt
     1501 cgtgcagggc ttccagcgcg aggacttgtt caccgtcgtg ctcgagcact tcatgaccga
     1561 cacggccgac catgccgact atgtactgcc ggccacgacc cagctcgagc acctggacgt
     1621 gcacagcagc tatggccaca cctatgcgct gatcaacgag cctgccatcg cgccgctggg
     1681 cgaggccctg cccaacagcg agatcttccg ccgcctggcc gcacgcatgg gctataccga
     1741 gccctgcttc ggcgagagcg acgagcagat cgccgcgcag gccttcggcg accacatcga
     1801 tttcgcccag ctgcgccgcg cgggctgggt caagctgccg ctgcccgaag cgccgttcgc
     1861 caacggcggc ttcccggtgc ccggcgggcg ctgccgcatc aacgcgccgg gcctgggcgt
     1921 gccggactat gtgccgccct acgagtccgc ggcctcggcg ccggagctgg cgcagcgcta
     1981 cccgctggcg atgatctcgc cgccggcgcg gcatttcctc aattccacct tcgtcaatgt
     2041 caagagcctg cgcagcgtcg aaggccagcc gttggtcgag atccacccca gcgatgccgc
     2101 cgcgcgcggc atcgccgacg gcgcgatggt gcgcgtctac aacgaccgcg gcgcataccg
     2161 ctgcgtggcc gagttgagcg agcgcgctcg cccaggcgtc gtcaacgggc tgggcatctg
     2221 gtggcgcaag ctgggcccgg acggcaccaa cgtcaatgaa gtgacgcacc agcgcctgac
     2281 cgacatcggg cgcgcgccca gcttctacga ctgcctggtc gaagtcgcgg cgctctgaag
     2341 gcgatgaggc ttgcgcgctg gctggcgatg ggaggcctgg gagcaatcgt catggcagcg
     2401 ctcgccgcgg ccacgctctg cctcaccagc ggctgcagca gtttcggcta cctggcgcaa
     2461 tccgcccatg ggcagctgag cctgatgagc gctgcgcgcc cggtgagcca agtgctggcc
     2521 gagccggaca cgagcgccga actgcgcgcg cggctggagc tgagccagcg catgcgcgac
     2581 tatgcgatcc gcgagctcaa gctgccagac aaccgcagct accgcagcta tgccgatctg
     2641 caccgcggcg ctgcggtctg gaacgtggtg gccgcgccag agctgagcct gcagctcaag
     2701 acctggtgct ttcccatcgt cggctgcgtg ggctatcgcg gctatttcga ccgcgcgagc
     2761 gccgatgccg cggccgatga gctcaggcac gaaggcgagg aggtcagcgt ctacggcgtg
     2821 ccggcctatt cgacgctggg ctggctcaac tggctgggcg gcgacccgct gctcaacacc
     2881 ttcatccgtt atcccgaagg cgagctggcg cgcatgatct tccacgagtt ggcgcaccag
     2941 gaggcctatg cggccggcga caccgagttc aacgaaagct tcgccacctg cgtcgagcag
     3001 atcggcggac gccgatggct gagcgagcag gccggcgccg cggcgcgcga ggagtacgag
     3061 cgctacgacg cgagacggcg cgacttccag gccctgacca tggcctatcg cgacaagctc
     3121 gacg
//
LOCUS       DASUCY010000397         2655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_988844, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000397 DASUCY010000000
VERSION     DASUCY010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_988844"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(349..1350)
                     /locus_tag="VFV25_07945"
     CDS             complement(349..1350)
                     /locus_tag="VFV25_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEY1393294.1"
                     /translation="MMTMIKRRTFVRACLGAGALLTRPGQAALRPDLHWQSRALVGFG
                     TTLSFQAAHENEALLTRALDAAVAAQRRIETQMSLFDAYSALSRLNRDGVLDAPPSEL
                     LEILQLSRAVSARSGGAFDVTVQPLWAAYATAQAQGRLPTPSEVAAARGRVDWRALEV
                     APRRLRFRGAGMGATLNGIAQGYAADRVRRVLAAHGIRHALIDAGEYSTLGHNAHDQP
                     WALAVADPRHEAAYVARLLADGRCLATSADDQTSFSADHRYHHIFDPHTGYSPPELAG
                     VTVAAPSGALADALTKVMFVAGVEGSLRLAKVWGVDVLLIDKAGRVRATAGMPIAKG"
     gene            complement(1347..>2655)
                     /locus_tag="VFV25_07950"
     CDS             complement(1347..>2655)
                     /locus_tag="VFV25_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEY1393295.1"
                     /translation="DANDHALLVVTRGDYSFVGENFVRGAVPDRLTLHQGQLPLELRD
                     LDLDEPLRLPPELSRADAKVFRVIGPAGLDPAQPLDLALRITRSKGMIYPERASRDFT
                     LRYRLPADYVQAPSSDNKSWRSIWVGRGWELALIAVALALLSVALARQSWLAANERRL
                     ARLRTTYLAFTLVFIGWYAQGQLSIVNLTALLQALIAKRSLDFFLYDPVSLVLWAFVA
                     ATLVIWGRGTFCGWLCPFGALQELVSQVAQRLGIARIKLHRRLDARLKLAKYAVLAVI
                     LATAVISEPWTDRVLEFEPFKTAITLAFVRSWPFVLWAGGVVLLSAFVYKGYCRYLCP
                     LGAWLALLGRLRLLRWIPRRAECGTPCQTCRHRCEYQAIRPNGAIVYEECLQCLDCVG
                     IHDDERRCAPLIARRHEGHKVFPIRPLPAAVSAAAQPAQGAAT"
BASE COUNT          445 a          904 c          939 g          367 t
ORIGIN      
        1 ccggcgggcg ggtaactttc tcttgcttgc ctgcccaaga gaaagtcacc aaagagaagg
       61 gcaccctact gtgctcggtc gccgctggac gcggcgactg gccctggggg ctggtggccc
      121 gagggcgggc aaagaactcg ccctgcgggc gcaaacagct ttgcccgacc gcgctgcgcg
      181 cggcaaccct cggaccactg cgttcctcgg cggcacagaa ggggacccgg acaaacggat
      241 tgacgagccg gccaagggcc ggcccacggc tgccgggcgc atatcccggc gcagcctcac
      301 gcagtgagac atcgcaacaa agaagcaccg gctgcaggac cagccagatc accctttagc
      361 aatcggcatc cccgccgtag ccctcaccct cccggccttg tcgatcaaca aaacatcgac
      421 cccccaaacc ttcgccagcc tgagcgaccc ctccacaccc gccacgaaca tcaccttggt
      481 cagcgcgtcc gccaacgcgc cgctcggcgc cgcaaccgtc acgcccgcga gctcgggcgg
      541 cgaatagccg gtgtgcggat cgaaaatgtg gtggtagcgg tgatccgcgc tgaagctagt
      601 ctggtcgtcg gccgaggtcg cgaggcagcg cccgtctgcc agcaggcgcg cgacataggc
      661 cgcttcgtgg cgcgggtcgg ccacggccag tgcccagggc tggtcgtgcg cgttgtggcc
      721 cagcgtcgag tactcgccgg catcgatgag ggcatggcgt atgccatgcg cggccagcac
      781 gcggcgcacg cggtcggcgg cataaccctg ggcgatgccg ttcagcgtgg cgcccatgcc
      841 ggcaccgcgg aagcgcagcc gtcgcggcgc gacttcgagt gcgcgccagt ccacacgccc
      901 gcgtgccgcg gccacttcgc tcggtgtcgg caatcggcct tgggcctgcg ctgtcgcgta
      961 cgcggcccac agtggctgca cagtcacgtc gaaggcaccg ccgctgcgtg ccgacacggc
     1021 gcgcgagagc tgcaggatct cgagcagctc ggacggcggc gcgtcgagca cgccgtcgcg
     1081 gttcaggcgt gacagcgcgc tgtaggcgtc gaacaaactc atctgcgtct cgatgcggcg
     1141 ctgcgcggcc accgcggcat cgagtgcgcg cgtgagcagc gcttcgttct cgtgcgcggc
     1201 ctggaagctc agcgttgtgc cgaacccaac cagggcacgg ctttgccaat gcagatcggg
     1261 cctgagcgcg gcctggcccg gccgcgtcag cagggctccg gcgccgaggc aggcgcgaac
     1321 gaaggtccgg cgcttgatca tcgtcatcat gtggcggctc cttgggcggg ctgggcggct
     1381 gcagagacgg ccgcgggcag ggggcggatg gggaagacct tgtgcccttc gtgccgcctc
     1441 gcgatcaagg gcgcacagcg ccgctcgtcg tcgtgtatgc ccacgcaatc caggcattgc
     1501 aagcattcct cgtagacgat ggcgccgttg gggcgaatgg cctggtattc gcagcggtgg
     1561 cggcaggtct ggcagggcgt gccgcattcg gcgcgccgcg gtatccaccg cagcagccgc
     1621 agccggccga gcagggccag ccaggcgcct agcggacaca ggtagcggca gtagcccttg
     1681 tagacgaagg cgctcagcag cacgacgccg ccagcccaca gcacgaaggg ccaggagcgc
     1741 acgaaggcca gggtgatggc cgtcttgaag ggctcgaact cgaggacccg gtcggtccaa
     1801 ggctccgaga tcacggccgt cgcgaggatc acggccagca cggcgtactt ggcgagcttg
     1861 agccgcgcgt ccagccttcg gtgcagctta atccgcgcga tgcccagacg ctgtgcgacc
     1921 tggctcacca gctcctgcag ggcgccgaag gggcagagcc agccgcagaa cgtgccacgg
     1981 ccccagatca ccagcgtcgc cgccacgaag gcccacagca ccaggctcac cgggtcgtag
     2041 aggaagaagt cgaggctgcg cttggcgatc agggcctgca gcagcgcggt caggttgacg
     2101 atggagagct gcccctgggc ataccagccg atgaagacca gcgtgaaggc aagataggtg
     2161 gtgcgaaggc gcgccagccg ccgctcgttc gcggcgagcc aggattgcct tgcgagcgcg
     2221 acgctcagca gcgccagcgc gacggcgata agcgcgagct cccagccgcg gccgacccag
     2281 atgctgcgcc aagacttgtt atcgctgctc ggcgcctgca cgtagtcggc cggcagccgg
     2341 tagcgcagcg tgaagtctcg gctggcgcgc tcggggtaga tcatgccctt gctgcgcgta
     2401 attcgcagcg caagatccag cggctgcgcg gggtccagcc cggcggggcc gatgacgcgg
     2461 aagaccttgg catcggcgcg gctcagctcg ggcggcagcc tcagcggttc gtcgagatcg
     2521 aggtcgcgca gctccagcgg cagctggccc tggtgcagcg tgaggcgatc gggcaccgcg
     2581 ccgcgcacga agttctcgcc gacgaagctg tagtcgccgc gggtgaccac gagtagcgca
     2641 tggtcgttgg cgtcg
//
LOCUS       DASUCY010000398         2983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1214019, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000398 DASUCY010000000
VERSION     DASUCY010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1214019"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..668
                     /locus_tag="VFV25_07955"
     CDS             111..668
                     /locus_tag="VFV25_07955"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEY1393296.1"
                     /translation="MYRTTIMLLATLLLAAVATDLRWRRIPNTLVLAGIAAAWILNAA
                     ASHSGSAALAGSAWWSSLAGLGLGLVLLLPLYLLGACGAGDVKLMAMVGAFVGARVVP
                     AVVLDTMLAGGLLSLAYMLARGVAAQTLSNVRFLLADWILRLTGGQATRLAPLQTTAA
                     RLPYAVAIAAGTAAALIWPLAGSMR"
     gene            665..2041
                     /locus_tag="VFV25_07960"
     CDS             665..2041
                     /locus_tag="VFV25_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY1393297.1"
                     /translation="MNLLQFPSGSSAHAATSADASAVRSALPRAPQTPEETGLSQTFL
                     VELLAKTMFQLGLTRLTELSAQLCLAGAVVEATCQFMRRESLLEVTRRGQHEADVQFD
                     LTQAGRARAIDWLARNSYVGAAPVPLELYADRVRAQSVTQQPVTVDQVRAAFADLVVS
                     ERLQDLLGTAVNSGRPMLLYGPAGSGKTYLAGHLHRLLTGSVAIPYAICVHGEVIRVF
                     DAHCHQQVAEEATPRAALDNRARPDARWVMCRRPCVVSGGELTLEMLDLSFDGRAGYY
                     EAPPHFKANNGLFIVDDLGRQVITPRELMNRWVVPMERRHDHLMLRNGGKFTIPFDMV
                     LVFSSNLRPAQLEDAAFLRRLGHKIAIDPVSLSEYSEIFERACADEGVACDAAAFRQL
                     VMDYHRPQGRPLLACYPRDLLHLIASRARYLGVQAELSKDLLDWAWQAYFGNEALIEA
                     ASEPGRPT"
     gene            2038..2898
                     /gene="cpaB"
                     /locus_tag="VFV25_07965"
     CDS             2038..2898
                     /gene="cpaB"
                     /locus_tag="VFV25_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEY1393298.1"
                     /translation="MRSRGLLMLLIAVLAGLAAVVVAARWMQQQAGQQGRIAVANADI
                     ELGGRISPEMVRMVDWPQASVPVGAFNDSDKLVGRVVMVSMQRGEPLIEGRLAPIGTK
                     GGLSAVVPEGKRAITVRVNDVVGVAGFALPGTYVDVMVSTQEDGAQNNNRDHTISKIV
                     LERILVLAVAQEADRDATKPKVVNAVTLEVTPKEAEMLDLARSVGTLSLVLRNQTDPK
                     PVVTTGVTKAELLGFVDKPAAEPAAKPAPTRVRHAALRATTPVLAAAPAAPRTCVEVI
                     RGLNKSTECF"
BASE COUNT          449 a         1051 c          970 g          513 t
ORIGIN      
        1 cgtcgtgatc atcgtgtctg tcacgtcggt cggccagaac ttggcggcgg tcttcaccgc
       61 cattgcagcc aacctggcag ccgtcctctg agccgcagac caagtgcacg atgtatcgca
      121 cgacgatcat gctgctcgcc accctcctgc tggccgccgt ggccaccgat ctgcgttggc
      181 ggcgcatccc caacacgctg gtgctcgcgg gcatcgcagc ggcgtggatc ttgaacgcgg
      241 ccgcatccca ctcgggcagc gcggccctcg ccgggtcggc ctggtggtcc agccttgccg
      301 gactaggcct gggcctcgtc ctcctgctgc cgctgtacct gctgggtgcc tgcggcgccg
      361 gcgacgtcaa gctgatggcc atggtcggcg ccttcgtcgg cgcgcgagtc gtgccggcag
      421 tggtgctcga caccatgctc gccggcggcc tgctgtcgct ggcctatatg ctggcccgcg
      481 gcgtggccgc acagaccttg agcaatgtgc gcttcctgct ggccgactgg atcctgcgtc
      541 tcaccggcgg ccaagcaaca cgcctcgcgc cgctgcagac cacggcggca cgcctgccct
      601 atgccgtcgc gattgcggcc ggaaccgcgg ccgcactgat ttggccactg gctgggagca
      661 tgcgatgaac ctgctccagt ttccgagcgg atcgagcgct cacgccgcta cgtctgccga
      721 cgcgtccgcg gtgcgcagcg ccctgccgcg agcgcctcag acgccggagg aaaccgggct
      781 ctcccagacc ttcctcgtcg agctgctggc caagaccatg ttccagctcg gcctgacccg
      841 gctcaccgaa ctcagcgccc agctctgcct ggccggcgcc gttgtcgagg ccacatgcca
      901 gttcatgcgc cgagaatccc tgctcgaggt gactcgccgc ggccagcacg aggccgatgt
      961 gcaattcgat ctgacccagg ccggccgtgc ccgcgccatc gactggctgg cgcgcaactc
     1021 ctatgtcggc gccgcgcctg taccgctcga gctttacgcc gatcgcgtgc gcgcccagtc
     1081 ggtcacgcag caacccgtca cggtggacca ggtgcgcgcc gcctttgccg acctggtggt
     1141 gtccgaacgc ctgcaggatc tgctcggcac ggccgtcaac tcggggcggc ccatgctgct
     1201 gtatggcccg gcgggttcgg gcaagaccta tctcgccggt catctgcaca ggctgctcac
     1261 cggatcggtg gcgattccct acgccatctg cgtgcacggc gaggtgatac gcgtcttcga
     1321 tgcccattgc catcagcagg tggcagaaga ggcgacgccg cgcgccgcac tggacaaccg
     1381 ggcccgcccc gatgcgcgtt gggtaatgtg ccggcggcct tgcgtcgtgt ccggcggcga
     1441 gctgacactg gagatgcttg acctgagctt cgacggccgc gccggctact acgaggcgcc
     1501 gccccacttc aaggccaaca acggcctgtt catcgtggac gacctggggc gccaggtcat
     1561 cacgccgcgc gagttgatga accgctgggt ggttccgatg gagcggcggc atgaccacct
     1621 catgctgcgc aacggcggca agttcaccat ccccttcgac atggtgctgg tgttctcttc
     1681 caatctgcgc cctgcccaat tggaagacgc ggccttcctg cgccggctgg gacacaagat
     1741 cgccatcgat cccgtgtccc tgagcgagta ctcggagatc ttcgagcgcg cctgtgcaga
     1801 cgagggcgtc gcatgcgatg ccgcggcatt tcgtcagctc gtcatggact atcacaggcc
     1861 ccagggccgt cccctgctgg cctgctatcc gcgcgacctg ctgcatctga tcgccagccg
     1921 cgcacgctac ctgggcgtgc aggccgagct gtccaaggat ctgctcgatt gggcctggca
     1981 ggcgtatttc ggcaacgagg cgctgatcga agcagcgtct gaacccggga gaccgacatg
     2041 agatcacgtg gcttgttgat gttgctgatt gccgtccttg cgggcctggc ggcggtcgtg
     2101 gtggctgcgc gctggatgca gcagcaagcc ggccagcagg gccgcatcgc cgtggccaac
     2161 gccgacatcg agctcggagg ccgcatctca cccgagatgg tccgcatggt cgattggccg
     2221 caggccagcg tgcctgtggg cgccttcaac gactccgaca agctggtggg ccgggtcgtg
     2281 atggtctcca tgcagcgcgg cgagccgctc atcgagggcc ggcttgcacc catcggcacc
     2341 aagggcggcc tctcggccgt agtgccagaa ggcaagcggg ccatcaccgt gcgcgtcaat
     2401 gacgtcgtgg gcgtggccgg cttcgcgctg cctggcactt atgtcgacgt gatggtgagc
     2461 acccaggagg acggcgccca gaacaacaac cgcgatcaca ccatctcgaa gatcgtgctc
     2521 gagcgcattc tggtgctggc tgtcgcgcag gaggccgacc gcgacgccac caagcccaag
     2581 gtggtcaacg cggtgacgct ggaagtcacg cccaaggagg cggagatgct tgacctggcc
     2641 cgcagcgtcg gcaccctgtc actggtgctg cgcaaccaga ccgaccccaa gccggtcgtc
     2701 accaccggcg tgacgaaggc cgagttgctg ggattcgtcg acaagcctgc cgccgagcct
     2761 gcggccaagc cggccccgac ccgcgtgcgc cacgcggctt tgcgagcaac cacgcccgtg
     2821 ctggccgccg cgccggccgc cccgcgcacc tgcgtcgagg tgatccgcgg actgaacaag
     2881 tcgactgagt gcttctaaac cgcgtgcttt gcgagaactc atccaaccgg gaggtcctca
     2941 tgtccatgtc ctcatgtgct ggctcccttg cccgctcggc cct
//
LOCUS       DASUCY010000399         4341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1005087, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000399 DASUCY010000000
VERSION     DASUCY010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005087"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VFV25_07970"
     CDS             complement(<1..353)
                     /locus_tag="VFV25_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase substrate-binding
                     domain-containing protein"
                     /protein_id="HEY1393299.1"
                     /translation="MPAKVLFGFHAVTVRLKTAPQSITELYVDAQRRDQRMRQFVERA
                     KETGVRLIESDDERLQRLSGTHRHQGVVAKVVPLPQAKSLDDLLDAIEGPPLLLVLDG
                     VTDPHNLGACLRVADG"
     gene            complement(381..1058)
                     /locus_tag="VFV25_07975"
     CDS             complement(381..1058)
                     /locus_tag="VFV25_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY1393300.1"
                     /translation="MSEGPIIAVERLSKQVSDSTGVLTILHDIDFSLAVQESVAIVGA
                     SGSGKSTLLSIIAGLDTPTSGTVKIGGRDIFAIDEDARAALRAARLGFVFQSFQLMSN
                     LTALENVMLPLELQGRSDARALAGEMLQRVGLGERLRHYPRVLSGGEQQRVALARAFV
                     VKPAVLLADEPTGSLDFATGETVMELMFQLNREEGTTLVLVTHDRGIAARCHRQVRIE
                     AGHMVNA"
     gene            1144..1740
                     /locus_tag="VFV25_07980"
     CDS             1144..1740
                     /locus_tag="VFV25_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEY1393301.1"
                     /translation="MGRSKAATKPVVVLVLGDSLSAEYGLKRGSGWVALMEQRIKQQK
                     PGTVVINASISGDTTAGGRSRLPALLDRQHPTHVIIELGGNDALRGLPLAMTEANLTA
                     MSQAVKASGAKPMLIGMQLPPNYGRQYTQDFSALYARVAKAEGAALVPFLLKGIADGP
                     DATDKWFQADRIHPREEAHPLMLANVWPVLWPWLNARP"
     gene            complement(1774..2391)
                     /gene="phaR"
                     /locus_tag="VFV25_07985"
     CDS             complement(1774..2391)
                     /gene="phaR"
                     /locus_tag="VFV25_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HEY1393302.1"
                     /translation="MTQARSEAKRRSDPEAPAASDKKSSAALRILKKYPNRRLYDTQT
                     SSYITLADVKQMVLDAENFVVRDAKSNEDLTRSILLQIILEEETGGVPMFSTSMLAHI
                     IRFYGHTMQGLMGSYLEKNIQAFIDMQNRLVEQSGGLGGKSFSPEVWTQFMNMQAPMM
                     QGLMSSYLDQSKNLFVQMQEQLQKQSESLFPGMPGFTPPGGGNKR"
     gene            complement(2501..3046)
                     /locus_tag="VFV25_07990"
     CDS             complement(2501..3046)
                     /locus_tag="VFV25_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY1393303.1"
                     /translation="MTSANASASIHIRRARPDPATVEALADVLVDCVEGGASVSFMHP
                     LPRAKAIGFWQGVLAGAERGERTVLVAEDAGSKAIVGTVQVMLSMPDNQPHRADIAKM
                     LVHRSARRRGVGEALMRAAEAVAREAGKTLLVLDTVTGGDAERLYARLGWQRCGEIPG
                     YALWPRGGLCSTTVFYQELAA"
     gene            complement(3123..>4341)
                     /gene="ligA"
                     /locus_tag="VFV25_07995"
     CDS             complement(3123..>4341)
                     /gene="ligA"
                     /locus_tag="VFV25_07995"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEY1393304.1"
                     /translation="RDALPFDIDGVVYKVNDRALQERLGFVTREPRWAVAHKYPAQEQ
                     MTRLRDIEVQVGRTGKLTPVAKLEPVFVGGTTVSNATLHNQDEITRKDVRIGDMVIVR
                     RAGDVIPEVVGVVLDAALNPGANQRSEPFDLYQRLQGQCPVCGSAITREEGEVDWRCS
                     GGLFCPAQRKQAILHFAGRRAMDVEGLGDKLVDQLVDNGIVRTLPELYKLGVAKLSAL
                     ERMADKSAQNLVDGLEKSKSTTLARFLFALGIRHVGETTAKDLARHFGALDRIMDASL
                     EQLLEVKDIGPTVAQSIRTFFDQPHNREVVEQLRAAGIHWEEHAGAAADASPKPLAGL
                     TLVLTGTLPTLTRDQAKEMIEAAGGKVAGSVSKKTSHVVAGEEAGSKLDKARELGVSV
                     LDEAGLLRLLQAP"
BASE COUNT          678 a         1475 c         1425 g          763 t
ORIGIN      
        1 ccgtcggcaa cgcgcagaca agcccccagg ttgtgcgggt cggtcacgcc atccagcacc
       61 agcagcagcg gcggcccttc gattgcatcc agcagatcgt ccagcgactt ggcctgcggc
      121 agcggcacca ccttcgccac cacgccctgg tggcgatgcg tgccgctgag cctctgcagc
      181 cgctcgtcgt cgctttcgat caggcgtacg ccggtttcct tggcgcgctc gacgaactgg
      241 cgcatgcgct ggtcgcggcg ctgcgcatcg acatagagct cggtgatgga ctggggcgcc
      301 gtcttcaggc gcacggtgac ggcgtgaaag ccgaacagaa ctttagcagg catcgcgcaa
      361 ttatcgggtg gcctcgatcc tcaggcattc accatatgtc ctgcctcgat gcgcacctgg
      421 cggtggcagc gcgcggcgat gccacggtca tgcgtcacca gcaccagcgt cgtgccctcc
      481 tcgcggttga gctggaacat cagttccatc acggtctcgc cggtggcgaa atccaggcta
      541 ccggtgggct cgtcggccag cagcaccgcg ggcttgacca cgaaggcgcg cgccagcgcc
      601 acgcgctgct gctcgccgcc tgacaagacc cgcgggtagt gcctgagccg ttcgcccagg
      661 ccaacgcgct gcagcatctc gccggcgagc gcgcgggcat ccgagcggcc ctgcagttcc
      721 agcggcagca tgacgttttc cagcgccgtc agattgctca tcagctggaa gctctggaac
      781 acgaagccga gccgggcggc acgcagcgcc gcgcgggcgt cctcgtcgat cgcgaagatg
      841 tcgcggcctc cgatcttcac cgtgccgctg gtcggcgtgt ccagccccgc aatgatggac
      901 agcagcgtac tcttgcccga gccggatgcc ccgacgatgg ccaccgactc ctgcaccgcc
      961 agcgaaaagt cgatatcgtg caggatggtc aagacgcccg tggagtcgct cacctgcttg
     1021 ctcaagcgct ctacggcaat gatgggtcct tcgctcatgt ccattcttgt tcgtactgca
     1081 gttgccccgg gtcggcggca ctttatcctg actgccttga ccctgtgctc gctcgccttg
     1141 actatgggca gatccaaagc ggcgacgaag cccgtcgtcg tgctggtgct gggcgacagc
     1201 ctgtctgccg aatacgggtt gaagcgcggc agcggctggg tcgcgctgat ggagcagcgg
     1261 atcaagcagc aaaagccggg caccgtcgtg atcaacgcca gcatcagcgg cgacacgacg
     1321 gccggcgggc gctcgcggct gcccgcgctg ctcgaccggc agcatccgac gcatgtgatc
     1381 atcgagctgg gcggcaacga tgccttgcgc ggcctgccgc tcgccatgac cgaggccaat
     1441 ctcaccgcga tgagccaggc cgtgaaggcc tcgggcgcca agcctatgct gatcggcatg
     1501 cagctgccgc ccaattacgg gcgacagtac acgcaggact tctcggcgct ctacgcccgg
     1561 gtcgcgaagg ccgaaggcgc cgccctcgtg cccttcctgc tgaaaggcat cgccgacggg
     1621 ccggacgcga ccgacaagtg gttccaggcc gaccgcatcc accccaggga agaagcgcac
     1681 ccgctgatgc tggccaatgt ctggccggtg ctgtggccct ggctcaacgc gaggccttga
     1741 tgccgtttgg atcaggcccc gctgcggggg ccttcagcgc ttgttaccgc cgccaggcgg
     1801 cgtgaagccg ggcatgccgg ggaataggct ctcgctctgc ttctgcagct gctcctgcat
     1861 ctgcacgaag aggttcttgg actggtcgag gtagctgctc atcaggccct gcatcatcgg
     1921 cgcctgcatg ttcatgaact gcgtccagac ctcggggctg aagctcttcc cgcccaaacc
     1981 gcccgattgc tcgaccaggc ggttctgcat gtcgatgaag gcctggatgt tcttctcgag
     2041 gtaggagccc atcaggccct gcatggtgtg tccgtagaag cggatgatgt gggccagcat
     2101 gctggtcgag aacatcggca cgccgccggt ttcttcctcg aggatgatct gcagcaggat
     2161 gctgcgcgtg agatcctcgt tgctcttggc atcgcggacg acgaagttct cggcgtcgag
     2221 caccatctgc ttgacgtcgg ccagcgtgat gtagctgctg gtctgcgtgt cgtagaggcg
     2281 gcgattgggg tacttcttga ggatacgcaa cgcggcagac gatttcttgt cgctggcggc
     2341 gggcgcttcg gggtcggaac ggcgtttggc ctcggacctg gcctgcgtca tgcaaagctc
     2401 cttgtgggac gaatcagcat tctaggaagc gcatttgcgc gtggcgcgaa gggtttcccc
     2461 ttgtgcgtct tgccgctgcg agccgcctat cggccagccg tcaggccgcc agctcctggt
     2521 agaacacggt tgtgctgcac aggccgcctc gcggccacaa cgcatagccg gggatctcgc
     2581 cgcaacgctg ccagcccagc cgcgcataga ggcgttcggc atcgccgccg gtcacggtat
     2641 ccagcaccag tagggtcttg ccggcctcgc gcgcaacggc ttctgcggcg cgcatcaagg
     2701 cctcgccgac gccgcggcgg cgcgcgctgc ggtgaaccag catcttggcg atatcggcac
     2761 ggtgaggctg gttgtcgggc atggacagca tgacttgcac cgtgccgacg atggccttgg
     2821 agcccgcatc ctcggccacc agcacggtgc gctcgcctct ttccgcgccg gccagcacgc
     2881 cctgccagaa accgattgcc ttggcgcgcg gcagcgggtg catgaagctc accgatgcgc
     2941 cgccctccac gcaatcgacc agcacatcgg ccagcgcctc gacagtggcc gggtcagggc
     3001 gcgcgcggcg tatgtggatg gaagcggagg cattggcgct cgtcatgcag atctcccgtt
     3061 catgaatgca aagagaaagc aagctgcgcg ccgggggcac agcggcagcg atgccgccgc
     3121 catcaaggcg cctgcagcaa gcgcagcagc ccggcctcgt cgagcacgct gacgccgagc
     3181 tctcgcgcct tgtccagctt gctgccggcc tcttcgccgg cgaccacatg gctggtcttc
     3241 ttggacaccg agcccgccac cttgccgccg gccgcttcga tcatctcctt ggcttggtcg
     3301 cgcgtcagcg tgggcaaggt gccggtgagc acgagggtca ggccggccag cggcttgggc
     3361 gaggcatcgg cagccgcgcc ggcatgctcc tcccaatgga tgcccgcggc gcggagctgc
     3421 tcgaccacct cgcggttgtg cggctgatcg aaaaaggtgc ggatgctctg cgctactgtg
     3481 gggccgatat ccttgacctc cagcagctgc tccaggctcg catccatgat gcgatcgagg
     3541 gcgccaaaat gccgcgccag atccttggcg gtggtctcgc cgacatggcg tatgcccagc
     3601 gcgaagagga agcgcgccag cgtggtggac ttgctcttct ccaggccgtc caccaggttc
     3661 tgcgcactct tgtcggccat gcgctccagc gccgagagct tcgccacgcc cagcttgtac
     3721 aactcgggca gcgtgcgcac gatgccgttg tccaccagct ggtcgaccag cttgtcgccc
     3781 aggccctcca cgtccatcgc gcggcggccg gcgaaatgca ggatggcctg cttgcgctga
     3841 gccgggcaga acagcccgcc ggagcagcgc cagtcgactt cgccttcctc gcgcgtgatc
     3901 gcgctgccgc acaccgggca ttgcccctgc aagcgctgat agagatcgaa gggctcgctg
     3961 cgctggttcg cgcccgggtt gagtgcggcg tcgagcacca cgccgacgac ctcgggaatc
     4021 acgtcgccgg cgcggcgcac gatcaccatg tcgccgatgc gcacgtcctt gcgcgtgatc
     4081 tcgtcctggt tgtgcagcgt ggcgttgctg accgtggtgc cgcccacgaa caccggctcc
     4141 agcttggcca ccggcgtgag cttgccggtg cggccgacct ggacttcgat gtcgcgcagc
     4201 cgggtcattt gctcctgggc cgggtacttg tgcgccacgg cccagcgcgg ttcgcgcgtc
     4261 acgaagccca ggcgctcctg cagcgcccgg tcattgacct tgtagacgac gccgtcgatg
     4321 tcgaagggca gcgcgtcgcg c
//
LOCUS       DASUCY010000400         2674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1486698, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000400 DASUCY010000000
VERSION     DASUCY010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1486698"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..143)
                     /locus_tag="VFV25_08000"
     CDS             complement(<1..143)
                     /locus_tag="VFV25_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEY1393305.1"
                     /translation="MQVSTYLKLSVVAAIVTIVLKTAAWWVTDSVGLLSDALESFVNL
                     AGAV"
     gene            complement(223..1401)
                     /gene="tyrS"
                     /locus_tag="VFV25_08005"
     CDS             complement(223..1401)
                     /gene="tyrS"
                     /locus_tag="VFV25_08005"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEY1393306.1"
                     /translation="MAISLRGCDELLPREDWLHKLARAEATGVPLRIKLGLDPTAPDL
                     HLGHTVVLNKMRQLQDLGHQVIFLVGDFTSMIGDPSGRNTTRPPLTREQIEANAQTYY
                     KQASLVLDSAKTEIRYNSEWSDPLGARGMIQLAAKYTVARMMERNDFHDRFKASTPIS
                     VHEFLYPLMQGYDSVALRSDLELGGTDQKFNLLMGRHLQAEYGQEPQCILTMPLLEGL
                     DGIEKMSKSKNNYIAITEPAPQMFAKLLSIDDTLMWKYFTLLSFRPEAEIARLREETK
                     AGRNPKEAKVMLAKEITARFHGAQAAEAAEADFNNRAKGGIPDDIPEVSLSGAPLGIG
                     ALLKQAGLVPSTSEGLRMVEQGGVKIDGATVSDKGLKLEAGRFVVQVGKRKFAQITLS
                     "
     gene            1692..>2674
                     /locus_tag="VFV25_08010"
     CDS             1692..>2674
                     /locus_tag="VFV25_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family peptidase"
                     /protein_id="HEY1393307.1"
                     /translation="MKSPHFLAQFFASAETVLKRHPRRLTAAVVITLLGSAVTAVAVA
                     PLAPDAAKLPRRVLTQSVSPVDLDSQLEALEQHELELYRSDLTRSSDTADTLLRRLGV
                     DDPEAAAFLRGDAVAHALLEGRAGKMVRVQTEGARLQQLIARSPAADADQAGKLFTRL
                     TITRDATGLHASTEQAALQTEVKLGSGTIRTSLFAAADDARIPDGVTTQLAELFGTDI
                     DFRRELQPGDTFSVLYEAPTADGEPITWNQASGRVLAAQFVNAGKSYSAVWFQEPGAA
                     KGAYFGLDGRSKQRFFLASPMAFSRITSGFAMRFHPIQHQWRAHKGVDYGAP"
BASE COUNT          422 a          898 c          886 g          468 t
ORIGIN      
        1 accgcgccgg cgagattgac gaaggactcc agcgcatcgg acagcaggcc caccgaatcg
       61 gtgacccacc aggccgccgt cttgagcacg atggtgacga tggccgccac caccgagagt
      121 ttgagataag tgctgacttg catggacgat gggaaattgc aacaagccta gccgaagccg
      181 gcggcatggc tgtgacgccg cgaaagactg gcggcagcgg tttcagctca gcgttatttg
      241 tgcaaacttt cgcttgccca cctgcacgac aaagcggccg gcctcgagct tgagcccctt
      301 gtcgctgacc gtggcaccgt cgatcttgac gccgccctgc tcgaccatgc gcaggccctc
      361 gctggtcgaa ggcacgagcc cggcctgctt gagcaaagcg ccgatgccca gcggcgcgcc
      421 ggaaaggctc acttcgggaa tgtcgtccgg gatgccgccc ttggcgcggt tgttgaagtc
      481 ggcctcggcc gcctcggcgg cctgcgcgcc gtggaagcgc gcggtgatct ccttggcgag
      541 catcaccttg gcttccttag ggttgcggcc ggccttggtc tcttcgcgca acctggcgat
      601 ctccgcctcg gggcggaagc tcagcagcgt gaagtacttc cacatcagcg tgtcgtcgat
      661 ggacagcagc ttggcgaaca tctggggcgc cggctcggtg atggcgatgt agttgttctt
      721 gctcttggac atcttctcga tgccgtccag gccctcgagc agcggcatgg tgaggatgca
      781 ctgcggctcc tggccgtatt ccgcctggag atgccggccc atcagcagat tgaacttctg
      841 gtcggtgccg cccagctcca ggtcgctcct gagtgccacc gagtcatagc cctgcatcag
      901 cgggtagagg aactcgtgca cgctgatcgg cgtgctggcc ttgaagcggt cgtggaagtc
      961 gttgcgctcc atcatgcgcg cgacggtgta cttcgcggcg agctggatca tgccgcgggc
     1021 gcccagcggg tcgctccatt ccgagttgta gcggatctcg gtcttggccg agtccagcac
     1081 caggctggcc tgcttgtaat aggtctgggc gttggcctcg atctgctcgc gcgtgagcgg
     1141 cgggcgggtc gtgttgcggc cggacgggtc accgatcatc gaggtgaagt cgccgaccag
     1201 gaagatcacc tggtgcccca ggtcctgcag ctggcgcatc ttgttcagta ccacggtgtg
     1261 gcccaggtgc aggtccggcg cagtcgggtc cagccccagc ttgatgcgca gtggcacgcc
     1321 ggttgcttct gcacgggcga gcttgtgcag ccagtcctcg cgcggcagca gctcgtcgca
     1381 gcccctcagg ctgatcgcca tggcctccag gactcggtcc gttaccgggt atttggacgc
     1441 cgatggcgac agttgcgaac ctgttgcggc ggatgcttga gcagggggct gggtcatggg
     1501 cctgatacgg gttattacag gcgagggcca cggagcggct ggatatactc cggacccttg
     1561 ccaggtggct ggaattctag tgggtcacgt gttcacgccc cgcatccggc cggctctaga
     1621 tcaacagggg cgcaccctga gcaaccgccg aacggcggtc agcgcagcgc ccaagaacct
     1681 ggaatccttg ggtgaagtct cctcatttct tggcgcagtt ctttgccagc gccgaaaccg
     1741 tactgaaacg ccatcctcgc cgcctcaccg cggcagtcgt catcacgctg ctgggcagcg
     1801 ccgtgacggc cgtggccgtc gcgccgctgg cgcccgatgc agccaagctg ccgcggcgcg
     1861 tgctgacgca aagcgtcagc cccgtcgatc tcgactccca gctagaagcc ctggagcagc
     1921 atgagctgga gctgtatcgt agcgacctga cgcgctcgag cgacacggcc gatacgctgc
     1981 tgcgccgcct gggcgtggac gatcccgagg ccgccgcctt cctgcgcggg gatgccgtgg
     2041 cccacgcgct gctggaaggg cgcgccggca agatggtgcg cgtgcagacc gaaggcgctc
     2101 gcctccaaca gctgatcgcg cgctcgccgg ccgccgacgc cgatcaggcc ggcaagctgt
     2161 tcacgcggct gacgatcacc cgcgacgcga ccggcctgca cgcgagcacc gaacaggcgg
     2221 ccctgcagac cgaagtgaag ctcggctccg gcacgatccg gacctcgctt ttcgccgccg
     2281 ccgacgatgc acgcatcccc gacggcgtga ccacgcagct ggccgagctg ttcggcaccg
     2341 acatcgactt ccgccgcgag ctgcagcccg gcgacacctt cagcgtgctt tacgaggcgc
     2401 ccaccgccga cggcgagccc atcacctgga accaggccag cggccgcgtg ctggcggcgc
     2461 agttcgtcaa tgctggcaag agctattccg ccgtgtggtt ccaggagccc ggcgcggcca
     2521 agggggccta cttcggcctc gacggccgca gcaagcagcg cttcttcctg gcgtcgccga
     2581 tggccttctc gcgcatcacc tcgggctttg cgatgcgctt ccaccccatc cagcaccagt
     2641 ggcgcgccca caagggcgtg gactacggcg cgcc
//
LOCUS       DASUCY010000401         3183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_956632, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000401 DASUCY010000000
VERSION     DASUCY010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956632"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VFV25_08015"
     CDS             complement(<1..271)
                     /locus_tag="VFV25_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEY1393308.1"
                     /translation="MLDRRDLLKAAAAAGLLPVGRYAMPAMAAVPPAAPAAPRRLIVV
                     FMRGAVDGLSVVVPYSEGDYYRARSTIALARPGQDGGVLDLDGRFG"
     gene            complement(300..1889)
                     /locus_tag="VFV25_08020"
     CDS             complement(300..1889)
                     /locus_tag="VFV25_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HEY1393309.1"
                     /translation="MKRMGTMGKAAAVCAAALGCLAASPVLAEPAATQAEALHVLNRL
                     GYGPAPGAVDYVMQIGVDRYIDEQLHPERIALPPALNQQLAKLTTSGETQRELVTRFR
                     EAQAAAKNDSEEGKAQRRELFQRIGYEAAEGRLLRAIESPRQLEEAMVDFWFNHFNVY
                     IGKNLDRVLVGNYEREAIRPYALGRFRDLLGATAHHPAMLYYLDNWLSVAPGFQPPRK
                     FGGPVAKSSGLNENYARELMELHTLGVDGGYTQKDVTELARMLTGWTINPRLSSGDSV
                     FYFDPRRHDNGSKEWLGQHVAGGGQAEGEWALDVLATHPSTARHISYELAQYFVSDQP
                     PQALVDRMAKRFLDSGGDIRAVLKTLFDSPEFRDPAVRGAKFKTPYQYLVSAVRASNL
                     QISNVRPLLGTMTQLGMPLYGCLTPDGYKNTEEAWLNPDAVTRRVNFATAFASGKLPF
                     ARPMDDGAPAVGPKALQRMAEKQDDAKVDPDWSTPPLAAHALLNTLGESISAKTRATV
                     AQSDQPLRAALVLGSPDFMRR"
     gene            complement(1983..2324)
                     /locus_tag="VFV25_08025"
     CDS             complement(1983..2324)
                     /locus_tag="VFV25_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393310.1"
                     /translation="MKKSLIVAGLLSAFALASFAQGTAATDKDTQWQKNHPRREQVNE
                     RLAKQNRRIKQEGKEGEMSKGQAAKLHKEDRQIRQEERDMAAQNGGHITKAEQKVLNQ
                     QENKVSQQIGK"
     gene            2516..>3183
                     /locus_tag="VFV25_08030"
     CDS             2516..>3183
                     /locus_tag="VFV25_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEY1393311.1"
                     /translation="MTEWIALALATLNLLLLLWLALRKDDGAAARTDGDELKAEFRNG
                     NAGLERGLRDELTRGLGSTRQELGSTLANFQQTLLTQQGDVARTQNEQIEGFRTQLAA
                     TQQLLADTLRASTASLTQQSQAAREAQDAALKRFADTLGEQLRSLSEANERRLSEVRT
                     TVEQRLIALQADNEKKLEQMRQTVDEKLHATLEQRLGESFKQVAERLEQVHRGLGEMQ
                     TLAQG"
BASE COUNT          472 a         1048 c         1106 g          557 t
ORIGIN      
        1 ggccgaagcg cccgtcgagg tcgagcacgc cgccgtcctg gccggggcgg gccagcgcga
       61 tcgtcgagcg ggcgcgatag tagtcgcctt ccgaataggg cacgacgacg ctcagtccgt
      121 ccactgcccc gcgcatgaag accacgatca gccggcgcgg cgcggcaggc gcagctggcg
      181 ggacggccgc catcgccggc attgcatatc ggcccacggg cagcaggccg gcggcggccg
      241 cggccttcag caagtctcga cgatccagca tctcgtttct cctcgtggcg cccgagcgct
      301 cagcgccgca tgaaatccgg gctacccagc accagggcgg cgcgcagggg ctggtccgac
      361 tgggcgaccg tcgcgcgcgt cttggccgag atgctctcgc ccagcgtgtt gagcagggcg
      421 tgggccgcaa gcggcggcgt ggaccagtcc gggtcgacct tcgcatcgtc ctgtttttcg
      481 gccatgcgct gcagcgcctt gggaccgaca gcaggcgcgc cgtcgtccat ggggcgcgcg
      541 aaggggagct tgccggacgc gaaggccgtc gcgaaattca cgcggcgcgt gaccgcatcc
      601 gggttgagcc aggcctcctc ggtgttcttg tagccgtccg gcgtcaggca gccgtacagc
      661 ggcatgccga gctgggtcat cgttccgagc agcgggcgca cattgctgat ctgcaggttg
      721 gacgcccgca cggcggatac gaggtattgg tagggcgtct tgaacttcgc gccgcgcacg
      781 gccggatcgc ggaattcggg gctgtcgaac agcgtcttca gcaccgcgcg tatgtcgccg
      841 ccgctgtcga ggaagcgctt ggccatgcga tcgaccagag cttgcggtgg ctggtcgctg
      901 acgaaatact gggccagctc gtagctgatg tggcgggcgg tggaagggtg agtcgcgagc
      961 acgtcgagtg cccactcccc ctcggcctgg ccgccgcccg ccacgtgctg ccccagccat
     1021 tccttgctgc cgttgtcgtg ccggcgcggg tcgaaataga agaccgagtc gccgctgctt
     1081 agtcgcgggt tgatggtcca gccggtgagc atgcgtgcca gttcggtcac gtccttctgc
     1141 gtgtagccgc cgtccacgcc cagggtgtgc agctccatca actcgcgtgc gtagttctcg
     1201 ttcagcccgc tggacttggc gacggggccg ccaaacttgc gcggcggctg gaagccgggc
     1261 gcgaccgaga gccagttgtc caggtaatag agcatggccg ggtgatgggc cgttgccccc
     1321 agcaggtcgc ggaagcggcc gagcgcatag gggcgtatcg cttcgcgctc gtagttgccg
     1381 accagcacgc ggtccaggtt cttgccgatg tagacgttga agtggttgaa ccagaagtcg
     1441 accatggcct cttcgagctg gcgcgggctc tcgatggcgc gcagcaggcg gccctctgcc
     1501 gcttcgtagc cgatgcgctg gaacagctcg cggcgctggg ccttgccttc ctcgctatcg
     1561 ttcttcgcgg cggcctgggc ctcgcggaag cgggtcacga gctcgcgctg cgtctcgccg
     1621 ctcgtcgtca gcttggcgag ttgctggttg agcgccggtg gcagcgcgat gcgctcgggg
     1681 tggagctgct cgtcgatgta gcggtcgacg ccgatctgca tcacgtaatc gaccgcgccg
     1741 ggggccgggc catagcccag gcggttgagc acatgcaagg cctcggcctg cgtggcggcc
     1801 ggctcggcca gcaccgggct tgcggccaag cagcccaagg ccgcggcgca gaccgccgct
     1861 gccttgccca tcgttcccat gcgcttcatt ggcgtgctcc ccggctcggt tgatcgatgg
     1921 atcagcgaga gcgctcatcg tgcgctctcg ctgaagggat ggggctccag ggccctttta
     1981 gctcacttgc cgatctgctg gctgaccttg ttttcctgct ggttcagcac cttctgctcg
     2041 gccttggtga tgtggccgcc gttctgcgcg gccatgtcgc gctcttcctg gcggatctgg
     2101 cggtcttcct tgtgcagctt ggccgcctgg cccttgctca tctcgccttc cttgccttcc
     2161 tgcttgatgc ggcggttctg cttggccagg cgctcattga cctgctcgcg gcgcggatga
     2221 ttcttctgcc actgggtgtc cttgtcggtg gcggccgtgc cttgcgcgaa gctggccagg
     2281 gcgaaggccg acagcaggcc ggcaacgatg agggacttct tcatgctcgt atctccttgg
     2341 tggttgtggc cgctctcgcg attgctttgc tggcctgagc ctccaacgag gcgcggcgaa
     2401 acgctgttga cgcccgattg tttctgccgc gcaaagcagg ctgacatgcc tgtcagcaga
     2461 gcttgtcgcc gcgcagcaaa agagtcggct tgcaggcctg agacaaaatc gccggatgac
     2521 cgaatggata gccctcgccc tggcgactct caatctcctg ctgttgctct ggctggctct
     2581 acgaaaagac gatggcgccg cggcgcggac cgatggcgac gaactcaagg cggaattccg
     2641 caacggcaat gcaggcctgg agcgcggcct gcgcgacgag ctgacgcgcg gcctgggcag
     2701 cacgcgccag gaactcggct ccacgctggc caacttccag cagacgctgc tgacccagca
     2761 gggcgatgtc gcccgcaccc agaacgagca gatcgaaggc tttcgcaccc agcttgccgc
     2821 aacgcagcaa ctgctggccg acacgctgcg cgccagcacc gccagcctga cccagcaatc
     2881 gcaggccgcg cgcgaggcgc aggatgcggc gctcaagcgc ttcgccgaca cgctcggtga
     2941 gcagctgcgc agcctctccg aggccaatga gcgccggctg tccgaggtgc gcaccacggt
     3001 cgagcagcgc ctcatcgcgc tgcaggccga caacgagaag aagctggagc agatgcggca
     3061 gaccgtggac gagaagctgc acgcgacgct ggagcagcgc ctgggcgaga gcttcaaaca
     3121 ggtggccgag cgcctggagc aggtgcaccg cggcctgggc gagatgcaga cgctggcgca
     3181 agg
//
LOCUS       DASUCY010000402         4959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_491008, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000402 DASUCY010000000
VERSION     DASUCY010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4959
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491008"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..659)
                     /gene="mog"
                     /locus_tag="VFV25_08035"
     CDS             complement(57..659)
                     /gene="mog"
                     /locus_tag="VFV25_08035"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HEY1393312.1"
                     /translation="MSETSLDTVRIGLVSVSDRASSGVYQDQGLPALRDWLGRALRNP
                     VEWRERLIPDEATTISASLRELVDAEGCQLVLTTGGTGPALRDVTPEATLAVADKTMP
                     GFGEQMRQISLHFVPTAILSRQVAVIRGRALIINLPGQPKSIAQTLEGLKGEDGRPAV
                     HGIFAAVPYCIDLIGGPYLETDPAVCAAFRPKSAQRPSRG"
     gene            complement(652..1203)
                     /gene="yjgA"
                     /locus_tag="VFV25_08040"
     CDS             complement(652..1203)
                     /gene="yjgA"
                     /locus_tag="VFV25_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HEY1393313.1"
                     /translation="MTLRDADPADERPSKTQLKKTMHELQELGEALLTLPDERLERIP
                     MPDTLREAVQALKRTRSHEGRRRQLQYVGKLMRGADVAPIREAVAAFRLGGAQATLAL
                     HQAERWRSELIADDAALTRWMDAYPQTDVQALRSLIRAARKDAPTGSAEPSAGERKGR
                     AYREIFQFIRQQLDANEPGAAHE"
     gene            1304..2683
                     /gene="pmbA"
                     /locus_tag="VFV25_08045"
     CDS             1304..2683
                     /gene="pmbA"
                     /locus_tag="VFV25_08045"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HEY1393314.1"
                     /translation="MPPTNTLDSQGFAYSQEQFRQLVEDSLGFAKELGASDAAVEVSE
                     GVGLSVSVRKGEVENVERNRDKSIGVTVYLGQRRGNASTSDFSRAALEQTVRAAYDIA
                     RFTADDPAAGLPDEADLALGEAARRDLDLFHPWAINAEEAVKIAQACEAAALATDARI
                     TNSEGAGVSAQQSHFWAGNSRGFRGGYASSRHSLSVAPIAGRGSEMQRDAWYSSMRAP
                     EDLAAPEAVGRYAAERALSRLRSRRIATCEVPVLFESTLAAGLLGGYVQATSGGALYR
                     KASFLPDSLGQSVFPEHIDIVEDPHVPKGKGSAPFDDEGVTTRARHVVQDGRVQGYFL
                     SSYSARKLNMRTTGHAGGSQNLKLESRLTQAGDDLEAMLRKLDRGLFVIELMGQGVNY
                     VTGDYSRGAAGFWVEGGRIVHPVDEITIAGNLREMFRQIVAVGADAYTLGTKTVGSVL
                     IERMKIAGR"
     gene            2824..3966
                     /locus_tag="VFV25_08050"
     CDS             2824..3966
                     /locus_tag="VFV25_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY1393315.1"
                     /translation="MKNKWLSILALAGAATAAYAADIKIGVAEALSGGAAQYGIAIRN
                     GFQLAADEINVAGGVNGNKLVLVIEDEQGKKEEAINVFKKLIFQDKVLMIFGPTLSNS
                     AQAADPIAQAAKTVVFGTSNTADGITSIGDHVFRNSVTEADVLPVTLKTSAAKAGIKK
                     VAVLYGNDDVFTKSGYDNFKKALEDLKIPVTTTETFAKGDVDFKAQLTKIKATNPDAI
                     VLSALVAEGAPIMVQARQLGINVPFIGGNGMNTVKVFDLAKDKAEGLWVGSPWSIENQ
                     ADANRKFIVAYTAKFKTAPDQFAAQAYDAMSIAVQAIRKIKLSGDLAAERAALREALP
                     GVKHVGATGAFAFRRAADKAGRPAGYDAQQTAIISVTRGGKFSIEK"
     gene            4030..4911
                     /locus_tag="VFV25_08055"
     CDS             4030..4911
                     /locus_tag="VFV25_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011003335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY1393316.1"
                     /translation="MFEQQLVNALSLGCVYALFALGFTLVFGVLGVINLSHGAVFMVG
                     AYAALSAVQHWNLPLWLALPCGALAGGLLGLLIDALALRRLRAQNAPHLIPMITTIGI
                     GIALTNAMQGWFGAQNMRFPQEMVPDSMLEWGALRLTTLELAIILLSFALMAVLLLVL
                     KRTQLGRALRAIAESPRAAWLLGINVEGLFMLTSFVAAALGGLAGVLIGLYSNAVFPL
                     MGQPMLHKGIAVIILGGMGDIRGAMFAGLFLGFAEVLSVAYIGSTMRDAVAFGLLFLI
                     LLVRPTGLFGKVQERKA"
BASE COUNT          772 a         1681 c         1721 g          785 t
ORIGIN      
        1 gacgcagcga gcaggaaggc caggacgacg caagggcggc gcatgcgcgg ggggtctcag
       61 ccgcgtgagg gccgctgcgc cgatttgggc cggaaggccg cgcagacggc cggatcggtc
      121 tccagatagg gcccgccgat gaggtcgatg caatacggca cggcggcgaa gatgccgtgc
      181 acggcaggcc tgccgtcctc gcccttcagg ccttccagcg tctgcgcgat ggacttgggt
      241 tggccgggca ggttgatgat caaggccctg ccgcgtatca ccgccacctg gcgcgacagg
      301 atggccgtgg gcacgaagtg caggctgatc tggcgcatct gctcgccgaa gccgggcatg
      361 gtcttgtcgg ccacggccag cgtggcttca ggcgtcacgt cgcgcagcgc cgggccggtg
      421 ccgccggtgg tcagcacgag ctggcagcct tccgcgtcca ccagttcgcg caggctggcg
      481 ctgatcgtcg tggcctcgtc cgggatcagg cgctcgcgcc attcgaccgg gttgcgcagt
      541 gcccgcccca gccagtcgcg cagcgcgggc aggccctggt cctgatagac gccggaagag
      601 gcgcggtcgc tcactgagac caggcctatc ctgaccgtgt cgaggctggt ttcactcatg
      661 ggcggctccc ggctcgttcg cgtcgagctg ctgcctgatg aactggaaga tctcgcgata
      721 ggcgcggccc ttgcgctcgc cggcggacgg ttcggcgctg ccggtcggcg catccttgcg
      781 ggccgcgcgg atcaggctgc gcagggcctg cacatcggtc tgcgggtagg cgtccatcca
      841 gcgcgtcagt gctgcatcgt cggcgatcag ctcgctgcgc cagcgctccg cctggtgcag
      901 ggccagcgtg gcctgggcgc cgccgaggcg gaaggccgcc accgcctcgc ggatcggcgc
      961 cacgtcggcg ccgcgcatca gcttgccgac atattgcagc tggcgccgcc ggccctcgtg
     1021 cgagcgggtg cgcttgagcg cctgcaccgc ctcgcgcaag gtgtcaggca tggggatgcg
     1081 ttccagccgc tcgtccggca gggtcaggag cgcctcgccg agctcctgca gctcgtgcat
     1141 cgtcttcttg agctgggtct tgctgggccg ctcgtcggcc gggtctgcgt cgcggagggt
     1201 caaggcgggc tcgttcaaaa ggaaatgggc aggcatagag cgttcgctat agcgctcgct
     1261 atgataggtt gcttgtcgac catcgaagct ccagaactcg aacatgccgc ccaccaacac
     1321 cctcgactcc caaggtttcg cttactccca ggagcagttc cggcagcttg tcgaggactc
     1381 gctgggcttc gcgaaggagc tcggcgccag cgatgcggca gtggaggttt ccgagggtgt
     1441 gggcctgtcg gtctcggtgc gcaagggcga ggtcgagaac gtcgagcgca atcgcgacaa
     1501 gtcgatcggc gtgacggtct acctgggcca gcggcgcggc aatgccagca cgtccgattt
     1561 ctcgcgcgcg gcgctggagc agaccgtgcg cgcggcctac gacattgcgc gcttcacggc
     1621 cgatgatccg gccgccggcc tgcccgacga ggccgacctg gcactgggcg aggcggccag
     1681 gcgcgacctc gacctcttcc acccctgggc catcaacgcc gaggaagcgg tgaagatcgc
     1741 ccaggcctgc gaggcggcgg cgctcgccac cgatgcgcgc atcaccaact ccgaaggggc
     1801 gggcgtctcg gcccagcagt cgcatttctg ggccggcaac agccgcggct tccgcggcgg
     1861 ctatgcgagc tcgcggcatt cgctgtcggt ggcgcctatc gcggggcgcg gcagcgagat
     1921 gcagcgcgat gcctggtaca gctcgatgcg cgcgcccgaa gacctcgctg cgccggaagc
     1981 cgtgggccgc tacgccgccg agcgcgcgct gtcgcggctg cggagccggc gcatagcgac
     2041 ctgcgaggtg ccggtgctgt tcgaatccac gctcgcggct ggcctgctgg gtggctatgt
     2101 gcaggccacc agcggcgggg cgctttatcg caaggccagc ttcctgcccg acagtctggg
     2161 ccagagtgtt tttcccgagc acatcgacat cgtcgaggac ccgcacgtcc ccaagggcaa
     2221 gggcagcgcg cccttcgacg acgagggcgt cacgacgcgc gcgcgccacg tggtgcagga
     2281 cgggcgcgtg cagggctatt tcctgtccag ctattcggcg cgcaagctca acatgcgcac
     2341 caccggccat gccggcggct cgcagaacct gaagctggag agccgcctga cccaggccgg
     2401 cgacgacctg gaggcgatgc tgcgcaagct cgaccgcggc ctcttcgtga tcgagctgat
     2461 gggccagggc gtgaactacg tgacgggcga ctattcgcgc ggcgccgccg gcttctgggt
     2521 cgagggtggg cgcatcgtgc atccggtgga tgagatcacg atcgccggca acctgcgcga
     2581 gatgttccgc cagatcgtgg ctgtgggggc ggacgcctat acgctgggca ccaagacggt
     2641 cggctcggtg ctgatcgagc gcatgaagat cgccgggcgc taggcccctc gaatgctgcc
     2701 gcggccggcg gcagcataaa ctcgcgcact gcgcgccgcg gcgcccgccg tgcaggagcg
     2761 ccgcgcggcc cgcacaaggc ccacagcccc ttctccctta acaccaagca cggaggcatg
     2821 cacatgaaga acaagtggtt gtccatcctc gccctggccg gtgccgccac ggcggcttac
     2881 gctgccgaca tcaagatcgg cgtggccgag gccctgtcgg gcggcgctgc gcagtacggc
     2941 atcgcgatcc gcaacggctt ccagctcgcg gccgacgaaa tcaatgtagc cggcggcgtc
     3001 aacggcaaca agctggtgct ggtgatcgag gacgagcagg gcaagaagga agaggcgatc
     3061 aacgtcttca agaagctgat cttccaggac aaggtgctga tgatcttcgg cccgacgctg
     3121 tccaattcgg cccaggccgc cgacccgatc gcccaggccg ccaagaccgt ggtcttcggc
     3181 acctccaaca cggccgacgg catcacctcg atcggcgacc acgtgttccg caactcggtg
     3241 accgaggccg atgtgctgcc cgtgacgctc aagaccagcg ccgccaaggc cggcatcaag
     3301 aaggtggcgg tgctctacgg caacgacgac gtcttcacca agagcggcta cgacaacttc
     3361 aagaaggcgc tggaagacct gaagatcccg gtcacgacga ccgagacctt cgccaagggc
     3421 gatgtggact tcaaggccca gctcaccaag atcaaggcta ccaatcccga cgccatcgtg
     3481 ctgtcggcgc tggtcgccga gggcgcgccc atcatggtgc aggcgcgcca gctgggcatc
     3541 aacgtgccct tcatcggcgg caacggcatg aacacggtga aggtcttcga cctcgccaag
     3601 gacaaggccg aaggcctgtg ggtgggcagc ccctggtcga tcgagaacca ggccgatgcg
     3661 aaccgcaagt tcatcgtcgc ctataccgcc aagttcaaga cggcgcccga tcagttcgca
     3721 gcccaggcct atgacgcgat gagcatcgcc gtgcaggcga tacgcaagat caagctcagc
     3781 ggcgacttgg cggccgagcg cgccgccctg cgcgaagcgc tgccgggcgt gaagcacgtg
     3841 ggcgcgaccg gcgccttcgc cttccgccgc gccgccgaca aggccggccg gcccgccggc
     3901 tacgatgccc agcagaccgc catcatcagc gtcacgcgcg gcggcaagtt ctcgatcgaa
     3961 aaatagatga gacggctcgc cgcgccgccg cgtgccggcg gtgcggcgat gcccagacgc
     4021 tcagctcaaa tgttcgaaca acagctcgtc aacgcgcttt cgctgggctg cgtctatgcg
     4081 ctgttcgcgc tcggcttcac gctggtcttc ggcgtgctgg gcgtgatcaa tctctcgcac
     4141 ggcgccgtct tcatggtcgg cgcctatgcg gcgctcagcg ccgtccagca ttggaacctg
     4201 ccgctgtggc tcgccttgcc ctgcggcgcg ctggccggcg gcctgctcgg cctgctgatc
     4261 gacgcgctgg cgctcaggcg gctgcgcgcg cagaacgcac cgcatctgat cccgatgatc
     4321 accacgatag gcatcggcat cgctctgacc aatgcgatgc agggctggtt cggcgcgcag
     4381 aacatgcgct ttccgcagga gatggtgccc gactcgatgc tggagtgggg cgccctgcgc
     4441 ctgaccacgc tggagctcgc catcatcctg ctgtccttcg cgctgatggc ggtgctgctg
     4501 ctggtgctca agcgcaccca gctcggccgc gcgctgcgcg ccatcgccga gtcgccgcgc
     4561 gcggcctggc tgctgggcat caatgtcgaa ggcctgttca tgctgacctc cttcgttgcc
     4621 gcggcgctcg gcgggctggc cggcgtgctg atcggcctgt actcgaacgc ggtcttcccg
     4681 ctgatgggcc agccgatgct gcacaagggc atcgcggtga tcatcctcgg cggcatgggc
     4741 gacatccgcg gcgcgatgtt cgcggggctg ttcctcggct tcgcggaggt gctgtcggtg
     4801 gcctacatcg gctcgaccat gcgcgacgcc gtggccttcg gcctgctgtt cctgattctg
     4861 ctggtgcggc ccacgggcct gttcggcaag gtgcaggagc gcaaggcatg agccccgcgc
     4921 ggccgcccga agaggcgaaa tccccctcgg ggggacggc
//
LOCUS       DASUCY010000403         2946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_314295, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000403 DASUCY010000000
VERSION     DASUCY010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314295"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VFV25_08060"
     CDS             <1..294
                     /locus_tag="VFV25_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393317.1"
                     /translation="GREKDRIDRVWLIDDEAPARAALLPALQGATVLRVKREALSAWL
                     APDAGQPMEAHFYLVDPMGNWMLRFPPAPEPAKVKRDVERLLRASASWDKAGR"
     gene            304..1470
                     /locus_tag="VFV25_08065"
     CDS             304..1470
                     /locus_tag="VFV25_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEY1393318.1"
                     /translation="MNGTPLYNLDPVAEMALLGLVLALGPLAWLWLRHRDGGVSAWLK
                     ALSWVTLFLTFDLVLFGAFTRLTDSGLGCPDWPGCYGNASPLGAHAEISLAQSALPSG
                     PVTHGKAWIEMIHRYLAVAVGLLIAVLMLASWWLRRRIATVSPLAATATFLWVCLQGA
                     FGALTVTLKLYPAIVTAHLLGGMVLLALLTWQATAHDDATPPLRITPGLRRGAAWVLA
                     LSALQIALGGWVSTNYAVLACGDFPTCQGSWWPSMDLDHGFTLLRGLGLGKDGGYLPF
                     AALTAIHFVHRLGALVLFCALSWLAWRLARQAHEEPRLRMFSAGVLAVALWQFATGLS
                     NVVLGWPLISAVAHTGGAAALVVLLTRLQAQARTARSRLHSPAAAPSALLREPT"
     gene            1491..2429
                     /gene="cyoE"
                     /locus_tag="VFV25_08070"
     CDS             1491..2429
                     /gene="cyoE"
                     /locus_tag="VFV25_08070"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEY1393319.1"
                     /translation="MSESLTSSAAHSLPLPSRMRQFFALTKPRVVQLIVFCAAIGMLL
                     AVPGWPSAAAWGRALAGSVGIWLVASAAAAFNCLVEQHIDARMKRTAWRPTAKGELTN
                     TQTLTFSALLCAAGMLVLHVWVNTLTMWLTFATFIGYAVIYTVVLKPLTPQNIVIGGA
                     SGAMPPVLGWAAMRGEVGAEAWVLCLIIFLWTPPHFWALALYRAEDYARAGLPMLPVT
                     HGNEFTRLQVLLYTLVLFAATLLPFVYGMSGWLYMAAAVALGLGFIGHAFRLWRSYSD
                     ALARKTFRFSILHLSLLFAALLLDHYLDPILKGWFA"
     gene            2426..>2946
                     /locus_tag="VFV25_08075"
     CDS             2426..>2946
                     /locus_tag="VFV25_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY1393320.1"
                     /translation="MKSIRRHLLLAAVVALGLAACKPAPQVAFKGVDITGADYARELN
                     LPDVDGKPRSLADFKGKAVLVFFGYTQCPDVCPTTMAELAEVKRQLGPDGERVQGVFV
                     TLDPERDTAALLKPYVAGFDPSFVALRGTAEQTQAAAKAFKVFYEKVPSKDGSGNYTL
                     DHTAAAYLFDPQGR"
BASE COUNT          388 a         1026 c          994 g          538 t
ORIGIN      
        1 ggccgcgaga aggaccgcat cgaccgcgtc tggctgatcg acgacgaggc gcccgcgcgc
       61 gcggccctgc tgccggcgct gcagggcgcc accgtgctgc gtgtcaaacg cgaggcgctg
      121 tcggcctggc ttgcacccga cgcaggccag ccaatggagg cccatttcta cctggtcgat
      181 ccgatgggca actggatgct gcgatttccg ccggcacccg agccagccaa ggtcaagcgc
      241 gacgtggagc gcttgttgcg cgcttcggct tcgtgggaca aagcaggacg ctgagccagc
      301 ggcatgaacg gcacaccgct ctacaacctc gacccggtcg ccgagatggc cctgctcggg
      361 ctggtgctcg cgctcgggcc cttggcctgg ttgtggctca ggcaccgcga tggcggcgtg
      421 tcggcctggc tgaaggcgct gagctgggtc acgctcttcc tcactttcga tctggtgctg
      481 ttcggcgcct tcacgcggct caccgattcc ggccttggtt gcccagactg gcccggctgc
      541 tacggcaatg ccagcccgct gggcgcgcat gcggagatca gcctggcgca aagcgcgctg
      601 cccagcgggc cggtgacgca cggcaaggcc tggatcgaga tgatccatcg ctacctggcc
      661 gtggccgtcg ggctgctgat cgcggtgctg atgctcgcga gctggtggct gcgccgccgc
      721 atcgccacgg tctcgccgct ggccgccacg gccaccttcc tgtgggtctg cctgcagggc
      781 gccttcggcg cgttgaccgt cacgctcaag ctctacccgg ccatcgtgac cgcgcatctg
      841 ctgggcggca tggtgctgct ggccctgctg acctggcagg cgacggcgca tgacgacgcg
      901 accccgccgc tgcgcatcac gcccgggctc aggcgcggcg cggcctgggt gctggccttg
      961 agcgctttgc agatcgcgct cggcggctgg gtcagcacca actacgcggt gctggcctgc
     1021 ggcgatttcc cgacctgcca gggctcatgg tggccgagca tggacttgga ccacggcttt
     1081 acgctgctgc gcggcctggg actgggcaag gacggcggct atctcccctt cgcggcgctg
     1141 acggccatcc acttcgttca caggctcggc gcgctggtgc tcttctgcgc cttgagctgg
     1201 ctggcctggc gcctggcacg ccaggctcac gaagagccgc gcctgaggat gttctccgcc
     1261 ggcgtgctgg cggtggcgct gtggcagttt gctaccggcc tcagcaatgt cgtgcttgga
     1321 tggccgctga tctcggcggt ggcacacacc ggcggcgctg ctgcgctggt ggtgctgttg
     1381 acgcggctgc aggcgcaagc taggaccgcg cgcagccggc tgcatagccc ggcggccgcg
     1441 cccagtgcct tgctgcgtga gcctacctag aattccgctc ttccttcgtc atgtccgagt
     1501 ccctcacctc ttcagccgcc cactcgctgc cgctgccttc gcgcatgcgg cagttcttcg
     1561 cgctgaccaa gccgcgcgtc gtgcagctca tcgtcttctg cgcggccatc ggcatgctgc
     1621 tggccgtgcc gggctggccc agcgccgcgg cctggggcag ggcgctggcg ggcagcgtcg
     1681 gcatctggct cgtcgccagc gcggcggcgg ccttcaactg cctggtcgag cagcacatcg
     1741 atgcgcgcat gaagcgcacc gcctggcggc ccaccgccaa gggcgagctg accaataccc
     1801 agaccctgac tttttcggcg ttgctatgtg cggccggcat gctggtgctg catgtctggg
     1861 tcaacacgct gacgatgtgg ctgacctttg ccaccttcat cggctatgcc gtgatctaca
     1921 ccgtcgtgct caagccgctg acgccgcaga acatcgtgat cggcggcgcc tcgggcgcga
     1981 tgccgccggt gctgggctgg gccgcgatgc gcggcgaggt cggcgccgag gcctgggtgc
     2041 tgtgcctcat catcttcctg tggacgccgc cgcatttctg ggccctggcg ctgtaccgtg
     2101 ccgaggatta cgcgcgcgcc gggctgccga tgctgccggt cacgcacggg aatgaattca
     2161 cgcgcctgca agtcttgctc tatacgctgg tgctgttcgc cgcgaccctg ttgcccttcg
     2221 tctatggcat gagcggctgg ctctacatgg ctgcggccgt ggcgctgggc ctgggcttca
     2281 tcggccacgc cttccggctg tggcggagct acagcgacgc gctggcgcgc aagactttcc
     2341 gattttccat cctgcatcta tcgctgctct tcgccgcgct gctgctggac cactatctcg
     2401 atcccattct gaagggctgg ttcgcatgaa gtccattcgc cgccacctgc tgctggctgc
     2461 cgtcgttgca ctggggcttg cggcctgcaa gccggcgccg caggtggctt tcaagggcgt
     2521 cgacatcact ggcgccgact acgcccgcga gctcaacctg cccgacgtgg acggcaagcc
     2581 acgcagcttg gccgacttca agggcaaggc cgtgctggtc ttcttcggct acacgcaatg
     2641 ccccgatgtc tgcccgacaa cgatggccga gctggccgag gtcaagcgcc agctgggccc
     2701 cgacggcgag cgcgtgcagg gcgtcttcgt gacgctcgat cccgagcgag acacggcggc
     2761 gctcctcaaa ccctatgtcg ctggcttcga cccgagcttc gtcgcgctgc gcggcacggc
     2821 ggagcagacg caagccgcag ccaaggcctt caaggtcttc tacgaaaagg tgccgagcaa
     2881 ggacggcagc ggcaactaca cgctggacca cacggccgcg gcctacctgt tcgacccgca
     2941 gggccg
//
LOCUS       DASUCY010000404         4453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_491041, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000404 DASUCY010000000
VERSION     DASUCY010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491041"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..142)
                     /locus_tag="VFV25_08080"
     CDS             complement(<1..142)
                     /locus_tag="VFV25_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEY1393321.1"
                     /translation="MNRAASPLAVFWRERQIRFSDCDPAGIVFYPQYFVMFNGLVEDW
                     VGE"
     gene            complement(139..1305)
                     /locus_tag="VFV25_08085"
     CDS             complement(139..1305)
                     /locus_tag="VFV25_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY1393322.1"
                     /translation="MPDHTYLDWPFFEERHRALARELDAWAAEHIAQAHAADVDAQCK
                     ALVKQLGAAGWLRHAVAGRGHGGAADAIDTRAICLIRETLARHSGLADFAFAMQGLGS
                     GAISLQGSEAQKRRWLPRVAAGEAISAFALSEPEAGSDVAALTCSARLDGAHYVLDGE
                     KTWISNGGIADVYVVFAHTGEAPGSRGISAFIVEADTPGLAIAERIAVIAPHPLARLR
                     FSNCHIPAANRIGAAGEGFKIAMRTLDVFRTSVAAAALGFARRALDEALRRAPSRKMF
                     KQTLADFQLTQAKLAQMATTIDSAALLTYRAAWQRDQGQSVTKAAAMAKMTATEGAQQ
                     VIDAAVQIWGGLGVVSEQPVERLYREIRALRIYEGATEVQQLIIARELLKEAGA"
     gene            complement(1319..2197)
                     /locus_tag="VFV25_08090"
     CDS             complement(1319..2197)
                     /locus_tag="VFV25_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HEY1393323.1"
                     /translation="MSTTPNNPAEPRVDPALLAGNRRSAADYRARHFGWQLADGVAHI
                     TLNRPERKNPLSFDSYAELRDLFAQVLRYADDAKAVVIAGAGDNFCSGGDVHEIIGPL
                     LRLKAPELLMFTRMTGELVKAMRACPQPIVAAVDGVCAGAGAILAMASDLRFGTARSK
                     TAFLFNRVGLAGCDMGACAMLPRLIGQGRASELLYTGRALGGEEAAQWGFFNRLCAPE
                     TLLDEATALACEIAAGPSFANGITKTMLHQEWAMSIDQAIEAEAQAQALCMLTEDFSR
                     AYQAFVAKQKPRFEGN"
     gene            complement(2194..2724)
                     /locus_tag="VFV25_08095"
     CDS             complement(2194..2724)
                     /locus_tag="VFV25_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY1393324.1"
                     /translation="MKRKTAPPAALDEASIGLEARVSSDDHMALKLWLRLLSCSTQVE
                     TEIRKRLRSRFGMTLARFDYLAQLSREPQGLRMNALSRYLMVTGGSITGLTDELEKEG
                     LVQRIPAPDDRRSFFLKLTDEGRRTFERIAAEHEVWVIELFSGLGLNERKTLYELLGR
                     LRLQMAAAQQTQGETA"
     gene            complement(2724..3500)
                     /locus_tag="VFV25_08100"
     CDS             complement(2724..3500)
                     /locus_tag="VFV25_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY1393325.1"
                     /translation="MATNTLQGRHAVVTGAARGIGAAIARSLAAEGARVTLMGRQLGA
                     LQASARELPGAGHKAVAVDVADAAAVALAFEQARAELGAIHVLVNNAGQAESAAFAKT
                     SPELWQRMLAVNLTGSFLCTQAALPDMLAAGWGRIVNIASTAALKGYAYVSAYVAAKH
                     GVLGLTRALALELAAKGITVNAVCPGYTETEILRESIARVVAKTGRSEAQARAEFAAG
                     NPQGRIVQPEEVADAVRWLCGDAASAITGQAISVSGGEVM"
     gene            complement(3506..>4453)
                     /locus_tag="VFV25_08105"
     CDS             complement(3506..>4453)
                     /locus_tag="VFV25_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003262850.1"
                     /note="catalyzes the conversion of salicylyl-CoA to
                     gentisyl-CoA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="HEY1393326.1"
                     /translation="LWNTQQRDAFRRIVDFVHANSEAKIAMQLGHAGAKGSTRVPWEG
                     EDLPLDQGNWPLISASPQQYLDGVSDWSRAMTRADMDRVRDDFVHSARLAVEAGFDWL
                     ELHCAHGYLLSSFISPLTNKRTDEYGGSLANRLRYPLEVFRALREVWPQQLPMSVRIS
                     AHDWVEGGITPDDAVEIAKAFKAASADLIDCSSGQVSKQQKPVYGRMFQTPFADRVRN
                     EAGIATIAVGAISEADHVNSIVAAGRADLCAVARPHLANPAWTLMEAAKIGYMDLAWP
                     KQYRSAKTQLERNLERERAMAAQTAGLSPLEQANRAQEV"
BASE COUNT          646 a         1514 c         1568 g          725 t
ORIGIN      
        1 cttcaccgac ccagtcctcc accaggccgt tgaacatcac gaaatactgt gggtagaaga
       61 cgatgccggc cgggtcgcag tccgagaagc ggatctgccg ttcgcgccag aacacggcca
      121 agggcgaggc ggccctgttc atgcgccggc ctccttcaac agctcgcgcg cgatgatgag
      181 ctgctgcacc tcggttgcgc cctcgtagat gcgcaaggcg cggatctcgc gatagaggcg
      241 ctccaccggc tgctcgctga ccacgcccag cccgccccag atctgcaccg cggcgtcgat
      301 gacctgctgc gcgccttcgg tggccgtcat cttggccatc gcggcggcct tggtgacgct
      361 ttgcccctgg tcgcgctgcc aggccgcgcg ataggtcagg agcgccgcgc tgtcgatggt
      421 cgtggccatc tgcgcgagct tggcctgggt gagctggaaa tcggccaggg tctgcttgaa
      481 catcttgcgc gagggagcgc gccgcagcgc ctcgtccagt gcccgccgtg cgaagcccaa
      541 tgcggccgct gcgaccgagg tgcggaagac atccagcgtg cgcatcgcga tcttgaagcc
      601 ttcgccggca gcgccgatgc ggttggcggc cgggatgtgg cagttgctga agcgcagccg
      661 cgccagcggg tggggcgcga tcaccgcgat gcgctcggcg atcgctaagc ccggcgtgtc
      721 ggcctcgacg atgaaggccg agatgccccg cgagcccggc gcttcgccgg tgtgggcgaa
      781 gaccacgtag acatcggcaa tgccgccgtt ggagatccag gtcttctcgc cgtcaagcac
      841 atagtgagcg ccgtccaggc gtgcggagca ggtcagcgcc gccacgtcgg agccggcttc
      901 gggctcggag agggcgaagg cactgatcgc ctcgccggcc gcgaccctgg gcagccagcg
      961 ccgcttctgg gcctcgctgc cttgcaggct gatggcgccg gagcccaggc cctgcatcgc
     1021 aaaggcgaaa tcggccaggc ccgaatgccg cgccagggtt tcgcggatca ggcagatggc
     1081 gcgcgtgtca atcgcgtcgg ccgcgccgcc atgtccacgc cccgccacgg catggcgcag
     1141 ccagcccgcg gcgccgagtt gcttgaccag ggccttgcac tgggcgtcga catccgccgc
     1201 gtgggcctgg gcgatgtgct cggccgccca ggcatccagc tcgcgagcca gggcgcggtg
     1261 gcgctcttcg aagaagggcc agtcgagata ggtgtggtcg ggcatggcgg gaccttgttc
     1321 aatttccttc gaagcggggc ttctgcttgg cgacgaaggc ctggtaggcg cgggagaagt
     1381 cctcggtcag catgcacagg gcctgggcct gggcctcggc ctcgatggcc tgatcgatgc
     1441 tcatagccca ttcctgatgc agcatggtct tggtgatgcc attggcgaaa ctggggccgg
     1501 ccgcgatctc gcacgccaat gcggtggctt cgtcgagcag ggtttcagga gcgcacaggc
     1561 ggttgaagaa gccccattgc gcggcctcct cgccgcccag cgcgcggccc gtgtagagca
     1621 gctcgctggc gcggccctgg ccgatgaggc gcggcagcat cgcgcaggcg cccatgtcgc
     1681 agccggccag gcccacgcgg ttgaacagaa aggccgtctt gctgcgagcc gtgccgaagc
     1741 gcaggtcgct cgccatcgcg aggattgcgc cggccccggc gcagacgcca tcgaccgccg
     1801 ccacgatggg ctgggggcag gcacgcatgg ccttgaccag ctcgcccgtc atgcgcgtga
     1861 acatcagcag ctcgggcgcc ttcaggcgca gcagcgggcc aatgatctcg tgcacatcgc
     1921 cgcccgagca gaagttgtcg cccgcgccgg cgatcacgac cgccttcgcg tcgtcggcgt
     1981 atctgagcac ctgggcgaac aggtcgcgca gctcggcgta ggaatcgaag ctcagcgggt
     2041 tcttgcgctc ggggcggttc agcgtgatgt gggcgacgcc gtcggccagc tgccagccga
     2101 aatggcgggc gcggtaatcc gctgcgctgc ggcggttgcc ggccagcagg gcggggtcga
     2161 cgcggggttc tgcagggttg ttcggtgttg tgctcatgcg gtttcgcctt gagtttgctg
     2221 ggcggcagcc atctgcaggc gtagccgccc cagcagttcg tacaaagtct tgcgttcgtt
     2281 gaggcccagg cccgagaaca gctcgatgac ccagacctcg tgctcggccg cgatgcgctc
     2341 gaaggtgcgc cggccttcat cggtgagctt gagaaaaaag gagcgccggt cgtccggcgc
     2401 gggaatgcgc tgcaccaggc cttccttctc cagctcgtcg gtgaggccgg tgatgctgcc
     2461 gccggtgacc atcaggtagc gcgacagcgc gttcatgcgc aggccttgtg gctcgcggga
     2521 gagctgggcc aggtagtcga agcgcgccag cgtcatgccg aagcggctgc gcaggcgctt
     2581 acggatttcg gtctcgacct gggtgctgca cgacagcagc cgcagccaga gcttgagcgc
     2641 catgtggtcg tccgagctga cgcgagcctc gaggccgatg ctggcttcgt cgagggcggc
     2701 aggcggcgcc gtcttgcgct tcatcacatc acctccccgc cggacacgga aatcgcctgg
     2761 ccggtgatgg ccgacgcggc atcgccgcac agccagcgca cggcatcggc cacttcttcc
     2821 ggctgcacga tgcggccctg cgggttgccg gctgcgaatt cggcgcgcgc ctgcgcctcg
     2881 ctgcgcccgg tcttggccac caccctggcg atgctctcgc gcaggatctc ggtttcggta
     2941 tagcccgggc agaccgcgtt gacggtgatg cccttggccg ccagctccag cgccagcgcg
     3001 cgcgtgaggc ccagcacgcc gtgcttggcg gcgacgtagg cgctgacata ggcatagccc
     3061 ttgagcgccg ccgtgctcgc gatgttgacg atgcggcccc agccggcggc cagcatgtcc
     3121 ggcaaggcgg cctgggtgca caggaagctg ccggtcagat tgaccgccag catgcgctgc
     3181 cacagctcgg gcgaggtctt cgcgaaggcg gcgctctcgg cttggccggc gttgttgacg
     3241 agcacatgga tcgcgcccag ctcggcacgg gcctgctcga aggccagtgc cacggccgcg
     3301 gcatcggcca catcgacggc cacggccttg tggcctgcac ccggcagctc gcgcgcgctg
     3361 gcctgcagcg cgcccagctg tcggcccatc agcgtgacgc gcgcgccctc ggcagcgagc
     3421 gagcgggcga tggccgcacc tatgccgcgc gcggcgccgg tcaccaccgc gtgccggcct
     3481 tgcagcgtat tcgtggccat gttcatcaga cctcttgtgc gcggttggcc tgctccagcg
     3541 gcgacagccc cgcggtctgc gcggccatcg cgcgctcgcg ctcgaggttg cgctcgagct
     3601 gagtcttggc cgatcgatac tgcttgggcc aggcgaggtc catgtagccg atcttcgcgg
     3661 cctccatcag cgtccaggcc gggttggcga ggtgaggcct ggccaccgcg cagaggtcgg
     3721 cacggccggc ggccacgatg ctgttcacat ggtcggcctc ggagatcgcg cccaccgcga
     3781 tcgtcgcgat gccggcctcg ttgcgcacgc ggtccgcgaa cggcgtctgg aacatgcgcc
     3841 cgtagaccgg cttctgctgc ttgctgacct ggcccgagga gcaatcgatc aggtcggcgc
     3901 tggccgcctt gaaggccttc gcgatctcga ccgcgtcgtc gggcgtgatg ccgccttcga
     3961 cccagtcgtg cgccgagatg cgcaccgaca tcggcagctg ctgcggccac acctcgcgca
     4021 atgcgcggaa tacctccagg ggatagcgca gccggttggc cagcgagccg ccgtattcgt
     4081 cggtgcgctt gttggtcagc ggtgagatga agctggagag caggtagccg tgcgcgcaat
     4141 gcagctccag ccagtcgaag cccgcttcga ccgccaggcg cgccgagtgg acgaagtcgt
     4201 cgcgcacacg gtccatgtcg gcgcgcgtca tcgcgcgcga ccagtcgctc acgccgtcga
     4261 gatactgctg cggcgaagcc gagatcagcg gccagttgcc ctgatcgagt ggcaggtcct
     4321 cgccttccca cggcacgcgg gtcgagccct tcgcgccggc atgacccagc tgcatcgcga
     4381 tcttcgcctc gctgttcgcg tgcacgaaat cgacgatgcg cctgaaggca tcgcgctgct
     4441 gcgtgttcca cag
//
LOCUS       DASUCY010000405         2950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1326257, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000405 DASUCY010000000
VERSION     DASUCY010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1326257"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1463)
                     /locus_tag="VFV25_08110"
     CDS             complement(<1..1463)
                     /locus_tag="VFV25_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEY1393327.1"
                     /translation="MRRDAARALGSAPPAEPVPTAAAAASARRSDWATLARLLPYLWH
                     YRVRVVLALAFLVGAKLANVAVPLLLKKLVDSLSIAPGSARAVLVVPVGLLVAYGLLR
                     LSTSLFTELRELVFAKATEGAARRISLETFRHLHALSLRFHLERQTGGMTRDIERGTR
                     AVNSLISYSLYSIFPTLVEVTLVLGLLAVKFDAAFAWITAAALVLYIAYTVTVTEWRT
                     QFRKQMNELDSKAHTRAIDSLLNYETVKYFGNEDFEAARYDESLERLRRAALKSQSTL
                     SMLNTGQQLIIATALIVMLWRATEGVVAGHLTLGDLVMINAFMIQLYIPLNFLGVIYR
                     EIKQSLTDLDKMFMLLAREREVADRPGAQDLHVDGATVRFEHVDFAYERDRPILHDVS
                     FEIPAGRKIAVVGPSGSGKSTLARLLFRFYDTQGGRITIDGQDLRDVTQASVRRAIGI
                     VPQDTVLFNDTIEYNIGYGEPGATHEQIEAAAKAAHI"
     gene            1595..1936
                     /locus_tag="VFV25_08115"
     CDS             1595..1936
                     /locus_tag="VFV25_08115"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEY1393328.1"
                     /translation="MPLPADANANGDIFGGWIMAQVDLAGAVLPARIARGRIATVAVN
                     QFVFKQAVSIGDLLSFYARVERIGNTSVTVHVEVYAERNPANPQVVKVTEASLTYVAI
                     DHDGRPRPLPR"
     gene            complement(1952..2782)
                     /locus_tag="VFV25_08120"
     CDS             complement(1952..2782)
                     /locus_tag="VFV25_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010110572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY1393329.1"
                     /translation="MNLKGKTLFITGGSRGIGLAIAKRAAADGANIAVIAKTTEENPK
                     LPGTIYSAAEEIIAAGGQALPVPGDIRDEASVLDAVARTVEQFGGIDILVNNASAISL
                     TPTHMTPMKRFDLMFGVNVRGTYLCTQACLPHLQKAAEAGRNPHVLNMSPPLAMREHW
                     FKNHVAYTMAKYGMSECTLGHAGEFKPYGIGVNSLWPRTAIATAALQMIPGVDPKACR
                     KPEILADAAYFILTSDARTTTGNFFIDDELLAKHGVTDLEQYSVVPGTKTFIPDFFVD
                     "
     gene            complement(2823..>2950)
                     /locus_tag="VFV25_08125"
     CDS             complement(2823..>2950)
                     /locus_tag="VFV25_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEY1393330.1"
                     /translation="QEAAARMDEEGEIFARMLREPAAREAMTAFMEKRKPDFSQW"
BASE COUNT          475 a          959 c         1013 g          503 t
ORIGIN      
        1 tggatgtgcg ctgccttggc cgccgcctcg atctgctcgt gcgtagcgcc cggctcgccg
       61 tagccgatgt tgtattcgat cgtgtcgttg aacagcaccg tgtcctgcgg cacgatgccg
      121 atcgcgcggc gcacgcttgc ctgggtcaca tcgcgcaggt cctggccgtc gatcgtgatg
      181 cgcccgcctt gcgtgtcgta gaagcggaac agcagccgcg ccagcgtcga cttgcccgag
      241 cccgaggggc cgaccacggc gatcttcctg ccggccggga tctcgaaact cacgtcgtgc
      301 aggatgggcc ggtcgcgctc gtaggcgaag tccacatgct cgaagcgcac ggtggcgccg
      361 tcgacgtgca agtcctgcgc gcccggccgg tcggccacct cgcgctcgcg cgccagcagc
      421 atgaacatct tgtccaggtc ggtcagcgac tgcttgatct cgcggtagat cacgcccagg
      481 aagttgagcg ggatatagag ctggatcatg aaggcgttga tcatcaccag atcgcccagc
      541 gtcaggtggc cggcgaccac gccctcggtg gcgcgccaca gcatgacgat cagcgcggtc
      601 gcgatgatga gctgctgccc ggtgttgagc atggacagcg tgctctgcga cttgagcgcc
      661 gcgcggcgca gccgctccag gctctcgtcg tagcgggcgg cctcgaagtc ctcgttgccg
      721 aagtacttga ccgtctcgta gttcagcagc gagtcgatgg cccgggtgtg ggccttggag
      781 tcgagctcgt tcatctgctt gcggaactgg gtgcgccatt cggtcaccgt aaccgtatag
      841 gcgatgtaga gcaccagcgc cgcggccgtg atccaggcga aagcggcgtc gaacttgacc
      901 gccagcaggc ccagcaccag cgtcacctcg accagcgtcg ggaagatgct gtagagcgaa
      961 taggagatca gcgaattcac cgcccgcgtg ccgcgctcga tgtcgcgcgt catgccgccg
     1021 gtctggcgct ccaggtggaa acgcaggctc agcgcatgca ggtggcggaa ggtctcgagc
     1081 gagatgcgcc gcgccgcgcc ctcggtggcc ttggcgaaga ccagctcgcg cagctcggtg
     1141 aagagcgaag tcgacaggcg cagcaggccg taggcgacca gcaagcccac cggcaccacc
     1201 agcacggcac gcgccgagcc gggcgcgatg gacaggctat ccaccagctt cttcagcagc
     1261 agcggcacgg cgacgttggc cagcttggcg ccgaccagga aggccagcgc cagcacgacg
     1321 cgcacgcggt aatgccacag atagggcagc aaacgggcca gcgtggccca gtcgctgcgg
     1381 cgcgcggagg ccgccgcggc cgcagttggc acgggttctg caggtggcgc gctgcccagc
     1441 gcgcgcgcgg cgtcacggcg catcgtgaaa caatcctggg agcaatttcg ttaagaaagt
     1501 ggaattcatg agcggaattt caagcagcct cacgcaggta aagccgctgg cgcccccgag
     1561 tttgccgcaa ggcctggaac tcgtgctgcg cgtcatgccg ctgccggccg acgccaatgc
     1621 caacggggac atcttcggcg gctggatcat ggcccaggta gacctggccg gcgccgtgct
     1681 gccggcgcgc atcgcccgtg gccgcatcgc aaccgtggcg gtcaaccagt tcgtgttcaa
     1741 gcaggccgtg tcgatcggcg acctgctgag cttctatgcg cgggtggagc gcatcggcaa
     1801 cacctcggtc acggtgcacg tcgaggtcta tgccgagcgc aacccggcca acccgcaggt
     1861 cgtgaaggtc accgaggcca gcctcaccta tgtggcgata gaccacgacg gccggccgcg
     1921 gccgctgccg cgctgagccc cggtcagcga atcaatccac gaagaaatcc gggatgaagg
     1981 tcttcgtccc cggcacgacg ctgtactgct ccaggtcggt gacgccgtgc ttggccagca
     2041 gttcgtcgtc gatgaagaag ttgccggtgg tcgtcctggc gtcgctggtg aggatgaagt
     2101 aggccgcgtc ggccaggatc tcaggcttgc ggcacgcctt gggatccacg cccgggatca
     2161 tctgcagcgc ggcggtggcg atcgcggtgc gcggccacag cgagttgacg ccgatgccat
     2221 agggcttgaa ttcgcccgca tggcccaggg tgcactcgct catgccgtac ttggccatcg
     2281 tgtaggccac gtggttcttg aaccagtgct cgcgcatggc cagcggcggc gacatgttca
     2341 gcacatgcgg gttacgtccc gcctcggcgg ccttctgcag gtgcggcagg caggcctggg
     2401 tgcacagata ggtgccgcgc acgttgacgc cgaacatcag gtcgaagcgc ttcatcggcg
     2461 tcatgtgcgt gggcgtgagg ctgatcgcgc tggcgttgtt gaccaggatg tcgatgccgc
     2521 cgaactgctc caccgtcctg gccactgcgt cgagcaccga ggcttcgtcg cggatgtcgc
     2581 cgggcaccgg caaggcctgg ccgccggccg cgatgatctc ctcggcggcc gagtagatgg
     2641 tgcccggcag cttggggttc tcctcggtgg tcttcgcgat gaccgcgatg ttggcgccgt
     2701 ccgccgccgc acgcttggca atggccaggc cgatgccgcg cgatccgccg gtgatgaaga
     2761 gggtcttgcc cttgaggttc atggtttctc ctggtgatca gcgactggtg actagtgact
     2821 gcttaccact gcgagaagtc cggcttgcgc ttttccatga aggcggtcat cgcctcgcgt
     2881 gccgccggct cgcgcagcat gcgggcgaag atctcgccct cctcgtccat gcgggcggcg
     2941 gcctcctgcg
//
LOCUS       DASUCY010000406         7288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_587401, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000406 DASUCY010000000
VERSION     DASUCY010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 7288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7288
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587401"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VFV25_08130"
     CDS             complement(<1..331)
                     /locus_tag="VFV25_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEY1393331.1"
                     /translation="MAKEYKDLVVGLDIGTAKVMAVVAEVLPNGELRVAGLGVASSHG
                     LKRGVVVNIDATVQSIQQALKEAEMMADCKITRVYTGITGSHIRGQNSTGMVIVRDKE
                     VTPVDVAR"
     gene            complement(341..1135)
                     /locus_tag="VFV25_08135"
     CDS             complement(341..1135)
                     /locus_tag="VFV25_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEY1393332.1"
                     /translation="MQATAPQAFDTRLLNAGANLLFALAALAFLGLALSWLVRLPLFT
                     LRGILIEGEVAHNSATTIRANAAPKLAGNFFTMDLARSQQAFESVPWVRRAVVRRVWP
                     NRLAVALEEHKAAAYWHREDRDDELVNSYGEVFDANLGDVEDESLPTLKGPEGSAGAM
                     LDFYRRLLPVFGRLGARVEALTLSTRGSWDAELDSGAEIELGRGTPDELLARAESFAG
                     TVTQVTSRYQRPLEYADLRHASGYAVKLRGVSTGAAADKQTKGARP"
     gene            complement(1147..2100)
                     /locus_tag="VFV25_08140"
     CDS             complement(1147..2100)
                     /locus_tag="VFV25_08140"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEY1393333.1"
                     /translation="MNIDPRSLGKVAVLMGGSSAEREVSFMSGKGVLAALQSQGVDAH
                     AFDPAERALDELKREGFARCFIALHGRHGEDGTVQGALELLGIPYTGSGVMASAIAMD
                     KVMTKRVWIAEGLPTPRYVWLAPEQQSRERVLTVPDELGLPLIIKPPREGSSIGVTKV
                     AGYSEMQDAVALSVKYDPDVLCEEFIEGEELTCPVLGAGATATALPVIRIVAPEGNYD
                     YQHKYFTDDTHYLCPSGLPEAEEREIQRIVVQAYRALSCRGWGRADLMLRKSDRKPFL
                     LEMNTSPGMTSHSLVPMSAKAAGMSYEQLCLHLIANAALDA"
     gene            complement(2097..3509)
                     /gene="murC"
                     /locus_tag="VFV25_08145"
     CDS             complement(2097..3509)
                     /gene="murC"
                     /locus_tag="VFV25_08145"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEY1393334.1"
                     /translation="MKHAVKHIHFVGVGGAGMSGIAEILLNLGYKISGSDQSDSATLR
                     RLAGLGVEAHVGHDAAHIVGADAIVTSTAVKADNPEVVAARAARVPVVPRAVMLAELM
                     RLKQGIAIAGTHGKTTTTSLVASVLAEAGLDPTFVIGGRLNSAGANSRLGSGDYIVVE
                     ADESDASFLNLMPVMAVVTNIDADHMDTYGHDVEQLRSAFVDFLHRMPFYGAAIVCAD
                     DPGVRAIVPRVSRPVVSYGFGADTQVRAVHVQALPGGQMRFVAQRRNGVRMPDLAITL
                     NLPGEHNVLNALAAIAVATELELPDAPVVKALAEFRGVGRRFQRYGEQPAAGSGHFTL
                     IDDYGHHPVEMAAVIAAARGAFPGRRLVLAFQPHRYTRTRDCFEDFVKVLGTADAVLL
                     AEVYAAGEAPIVAADGRALARALRVAGKVDPLFVDDIAAMPQAIAEQARDGDVVITMG
                     AGSIGAVSAQVVERLKGKAV"
     gene            complement(3589..4683)
                     /gene="murG"
                     /locus_tag="VFV25_08150"
     CDS             complement(3589..4683)
                     /gene="murG"
                     /locus_tag="VFV25_08150"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEY1393335.1"
                     /translation="MTRHLVIMAAGTGGHIIPGLAVAREMERRGWTVSWLGTSQGMEN
                     RLVPAQGIAMDVIAFNGLRGKGLLHTLTGGLRLLGAFWACLKILRARTASAVLGMGGY
                     VCFPGGLMAALLGKPLMLVNADASLLMSNRALLPVADRVGFGFDGAAATTIKRATVTG
                     NPVREEIEALPSPGERYAGRSGPLRVLVVGGSLGAKVLNETLPAALALIAPGERPSVT
                     HQTGQLNRDAVKAAYAQAGVTEGVEVLPFIDDMATRLADCDLLICRAGAITVSEICAG
                     GIAAMLVPLIVSTTSHQRDNAQFMAQHGAAMHLPQAELTPQKLAEMLRGLDRTQLQAM
                     AEKARSLARPRAAARVADELDRLVNKGTAA"
     gene            complement(4680..5861)
                     /gene="ftsW"
                     /locus_tag="VFV25_08155"
     CDS             complement(4680..5861)
                     /gene="ftsW"
                     /locus_tag="VFV25_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEY1393336.1"
                     /translation="MPVRDWVSVSSGEPARLLGFDQSLLWLSIALLALGLVMVYSASV
                     ALPDNPRFAHYAPTHFLTRQALFIAIGLIAGAITVQLPIQVWEKSAPWLFVVALLLLM
                     VVLVPFIGKGVNGARRWIPLGVMNFQPSELAKLAIALYAADYMVRKMEIKENFVRAVA
                     PMAVAVAVIGLLLLAEPDMGAFMVIAAIAMGILFLGGVNGRMFLLITAVLVGAFVLMI
                     TFSEWRRERIFAYLNPWDEKYSLGKAYQLTHSLIAFGRGELLGQGLGGSVEKLYYLPE
                     AHTDFLLAVIGEELGFAGVTVVIFAFFWLTRRIFHIGRQAIALDRVFSGLLAQGIGIW
                     MGGQAFINMGVNLGVLPTKGLTLPLMSYGGSAVVLNCVALAIVLRVDIENRQLMRGGR
                     A"
     gene            complement(5951..>7288)
                     /gene="murD"
                     /locus_tag="VFV25_08160"
     CDS             complement(5951..>7288)
                     /gene="murD"
                     /locus_tag="VFV25_08160"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEY1393337.1"
                     /translation="KTTTTMMAGALVARAGKRAAVAGNVGPTMLQTLAEALDLEPEVP
                     VEQAQEAAPAESVEAPSSDAPTPAPSASQEAMEAEVDTGAEAAPPEDEDAVPLQLAPP
                     PPAAPRFEHLPEVWVLELSSFQLDGAQGFEPSAAAVLNVTQDHLDWHGDMPAYAAAKA
                     RIYGQQAVMVVNRDDPLVEAMVPAPIPVKGRNAKPITRRVVRFGLDAPQHPGDFGLVI
                     ENGMAWLVRAMEADETVKRRKDEVEELHIQRLMPADALRVRGRHNAANALAALALAVS
                     VGCPLAPMLHGLREYAGEPHRVEYLGRVGEVEAYDDSKGTNVGATVAALNGLGADKAP
                     AKLLVILGGEGKGQDFSPLLEPVSQHVRAAALIGRDAEALDVALGAAGVPLQRHDSLE
                     AATRWCFEQAAPGDAVLLSPACASLDMFRNYAHRAEVFKDTVRSLAAERGEML"
BASE COUNT         1126 a         2539 c         2490 g         1133 t
ORIGIN      
        1 cgcgcgccac gtccaccggc gtcacttcct tgtcgcgcac gatcaccatg ccggtgctgt
       61 tctgcccgcg gatgtggctg ccggtgatgc cggtgtagac gcgggtgatc ttgcagtcgg
      121 ccatcatctc ggcttctttc agcgcctgct ggatggactg cacggtggca tcgatgttga
      181 ccaccacgcc gcgcttgagc ccgtgcgagg aggccactcc cagacctgcc acgcgcagct
      241 cgccgttggg cagcacctcg gccaccaccg ccatcacctt ggcggtgccg atgtccagtc
      301 cgacgacgag atccttgtat tccttggcca tgaggcttcc ttagggtcta gcgccttttg
      361 tctgcttgtc cgctgccgcg ccggtcgaga cgccgcgcag cttcaccgca tagcctgaag
      421 cgtggcgcag gtcggcgtat tccagcggcc gctggtagcg cgaagtcacc tgcgtcacgg
      481 tgccggcgaa gctctcggcg cgcgccagca gctcgtccgg cgtgccgcgc ccgagctcga
      541 tttccgcgcc gctgtcgagc tcggcatccc aggagccgcg tgtggacagc gtcagcgctt
      601 cgacgcgcgc gcccagtctc ccgaacacag gcagcaggcg gcggtagaag tcgagcatcg
      661 cgcccgcgct gccctccggc cccttcagcg tggggagcga ttcgtcctcg acatcgccca
      721 ggttggcgtc gaagacctcg ccatagctgt tgaccagctc gtcgtcgcga tcctcgcgat
      781 gccagtaggc ggctgccttg tgttcttcca gcgccaccgc gagccgattg ggccagaccc
      841 gcctgaccac ggcacggcgc acccagggca ccgattcgaa ggcctgctgg ctgcgcgcca
      901 ggtccatcgt gaagaagttg ccggccagct tgggcgcggc attggcgcgt atcgtcgttg
      961 cgctgttgtg cgccacctcg ccctcgatca ggatgccgcg cagcgtgaac aacggcaacc
     1021 gcaccagcca ggacagcgcc aggcccagga acgccagcgc cgccagcgcg aacagaaggt
     1081 tcgcgccggc gttgagcagc cgggtgtcga aggcttgggg cgcagtggct tgcacggtct
     1141 gctttcctag gcgtctagtg cggcgttggc gatcaggtgc aggcagagct gctcgtagct
     1201 catgccggcg gccttcgcgg acatcggcac cagcgaatgc gaggtcatgc ccggcgaggt
     1261 gttcatctcg agcaggaagg gcttgcggtc gctcttgcgc agcatcaggt cggcccgacc
     1321 ccagccgcgg cagctgagcg cccggtaggc ctgcacgacg atgcgctgga tctcgcgctc
     1381 ttcggcctca ggcaggccgc tcgggcagag gtaatgcgtg tcgtcagtga aatacttgtg
     1441 ctggtagtcg tagttgccct cgggcgcgac gatgcgtatc accggcagtg ctgttgccgt
     1501 ggcgcctgcg cccagcaccg gacaagtcag ctcctcgcct tcgatgaact cctcgcacag
     1561 cacgtcgggg tcgtatttga cggacagggc cacggcatcc tgcatctccg aatacccggc
     1621 gaccttggtg acgccgatcg acgagccctc gcgcggcggc ttgatgatga gcggcaggcc
     1681 caactcgtca ggaaccgtaa gcacgcgctc gcgcgattgc tgctcggggg cgagccagac
     1741 atagcgcggc gtcggcagac cttcggcgat ccacacccgc ttggtcatga ccttgtccat
     1801 cgcgatggcc gaggccatca cgcccgatcc cgtgtaggga atgcccagca gctcgagcgc
     1861 gccctgcacc gtgccgtctt cgccgtggcg gccgtgcagt gcgatgaagc aacgcgcaaa
     1921 gccttcacgc ttgagctcat ccagcgcgcg ctcggcggga tcgaaggcat gcgcgtccac
     1981 gccctgcgat tgcagggcgg cgagcacgcc cttgccggac atgaaggaca cttcgcgctc
     2041 ggccgagctg ccgcccatca gcaccgccac cttgccgagc gagcgtggat cgatgttcat
     2101 actgctttcc ccttgagccg ctcgaccacc tgggccgata ccgcgccgat cgagccggcg
     2161 cccatcgtga tcaccacgtc gccgtcgcgc gcctgctcgg caatggcctg cggcatggct
     2221 gcgatgtcgt cgacgaacag cgggtcgacc ttgcccgcca cgcgcagcgc gcgcgccagc
     2281 gcgcggccgt cggccgcgac gatgggtgcc tcgccggccg catagacctc ggccagcagc
     2341 accgcatcgg ccgtgcccag aaccttgacg aagtcctcga agcaatcgcg cgtgcgcgtg
     2401 tagcggtgcg gctggaaggc cagcaccagg cgccgccccg ggaatgcgcc gcgcgccgcg
     2461 gcgatcacgg ccgccatctc gaccgggtga tggccgtagt cgtcgatcag cgtgaaatgc
     2521 ccgctgcccg ccgcgggctg ctcgccgtag cgctggaagc gccggcccac gccgcggaat
     2581 tccgccaggg ccttgaccac cggtgcatcg ggcagctcca gctcggtggc gacggcgatg
     2641 gccgccagcg cgttcagcac gttgtgttcg ccaggcaggt tgagcgtgat cgccaggtcg
     2701 ggcatgcgca cgccgttgcg gcgctgggcc acgaagcgca tctggccgcc cggcagggcc
     2761 tgcacgtgca ccgcgcgtac ctgggtgtct gcaccgaagc cgtagctcac caccgggcgc
     2821 gagacccgcg gaacgatggc gcgcacgccg ggatcgtcgg cgcagacgat ggccgcgccg
     2881 tagaaaggca tgcggtgcag gaagtccacg aaggccgatc tcagctgctc gacgtcatgc
     2941 ccataggtgt ccatgtggtc ggcgtcgatg ttggtgacga ccgccatcac cggcatcagg
     3001 ttcaggaagg aggcgtcgga ctcgtcggcc tcgaccacga tgtagtcgcc cgaacccagg
     3061 cgcgagttgg cgccggcact gttgagccgg ccaccgatca cgaaggtcgg gtccagcccg
     3121 gcctcggcca gcacgctggc gaccagcgaa gtcgtcgtcg tcttgccatg ggtgccggcg
     3181 atcgcgatgc cctgcttcag gcgcatcagc tcggccagca tcacggcgcg cggcaccacc
     3241 ggcacgcgag cggcgcgcgc cgccacgacc tcggggttgt cggccttcac cgcggtggag
     3301 gtgacgatgg catcggcccc gacgatatga gcggcatcgt gcccgacatg ggcctccacg
     3361 cccaggccgg cgagacggcg cagcgtggcg ctgtcgctct ggtccgagcc cgagatcttg
     3421 tagccgaggt tgagcaggat ctcggcgatg ccggacatgc cggcgccgcc gaccccgacg
     3481 aagtggatgt gcttgactgc gtgtttcatg cgcctctcct cacatgcaga ggctgcttcg
     3541 tcgggactcc tgatccttcg acgaagtgga tgtgcttgac tgcatgcttc atgcggcagt
     3601 ccccttgttg accagacggt ccaattcgtc cgcgacgcgg gccgccgcgc gcgggcgggc
     3661 cagcgagcgg gccttctcgg ccatcgcctg cagttgcgtt cggtccaggc cgcgcagcat
     3721 ctcggccagc ttctgcggcg tcagctcggc ctgcggcagg tgcatcgccg cgccatgctg
     3781 cgccatgaac tgcgcgttgt cgcgctgatg cgaggtggtg ctgacgatca gcggcaccag
     3841 catcgccgcg atgccgccgg cgcagatctc gctgaccgtg atcgcgcccg cgcggcagat
     3901 cagcaggtcg caatcggcca ggcgcgtggc catgtcgtcg atgaagggca gcacttcgac
     3961 gccttcggtg acgccggcct gggcgtaagc ggccttcacg gcatcgcggt tgagctggcc
     4021 ggtctggtgc gtgacgctgg gccgttcgcc tggcgcgatc agcgccagcg ctgcgggcag
     4081 ggtctcgttg agcaccttgg cgcccaagct gccgccgacc accagcacac gcagcgggcc
     4141 gctgcggccc gcatagcgct cgccgggcga gggcagggcc tcgatctctt cgcgcaccgg
     4201 attgccggtg accgtggcgc gcttgatggt cgtggccgcc gcgccgtcga agccgaagcc
     4261 gacgcggtcg gccaccggca gcagcgcgcg gttgctcatc agcagcgagg cgtcggcatt
     4321 gaccagcatc agcggcttgc cgagcagggc tgccatcagg ccaccgggaa agcagacata
     4381 gccgcccatg cccagcacgg cggacgccgt gcgtgcgcgc aggatcttca ggcaagccca
     4441 gaaggcgccg agcaggcgca gaccgccggt cagcgtatgc agcaggccct tgccgcgcag
     4501 gccattgaag gcgatcacgt ccatcgcgat gccctgggcc ggcaccaggc ggttctccat
     4561 gccctggctc gtgcccagcc agctcacggt ccagccacgg cgctccatct ctctggccac
     4621 agcgaggccg gggatgatgt gaccacccgt gcccgcggcc atgatgacga ggtggcgggt
     4681 catgctctgc ctccccgcat caattgcctg ttttcgatat cgacacgcaa cacgatcgcc
     4741 aaggcaacgc agttgagcac caccgccgag ccgccatagc tcatcagcgg cagcgtcaag
     4801 cccttggtcg gcagcacgcc caggttcacg cccatgttga tgaaggcctg gccgcccatc
     4861 cagatgccga tgccctgggc cagcaggccg gagaagacgc gatccagcgc gatggcctgg
     4921 cggccgatgt ggaagatgcg tcgcgtgagc cagaagaagg cgaagatcac gacggtgacg
     4981 ccggcgaagc cgagttcctc gccgatcacg gcgagcagga agtcggtgtg cgcttcgggc
     5041 aggtagtaga gcttctcgac gctgccaccc aggccctggc ccagcagctc gccgcggccg
     5101 aaggcgatca gcgagtgggt cagctgatag gccttgccca ggctgtactt ctcgtcccag
     5161 ggattgaggt aggcgaagat gcgctcgcgc cgccattcgc tgaaggtgat catcagcacg
     5221 aaggcgccga ccagcacggc cgtgatgagc aggaacatgc gtccgttgac gccgcccagg
     5281 aagaggatgc ccatcgcgat cgccgcgatc accatgaagg cgcccatgtc gggctcggcc
     5341 agcagcagca ggccgatcac ggccaccgcc acggccatcg gtgcgacggc gcggacgaag
     5401 ttctccttga tctccatctt gcggaccatg tagtccgccg catagagcgc gatggcgagc
     5461 ttggccagtt ccgagggctg gaagttcatc acgcccagcg ggatccagcg gcgcgcgccg
     5521 ttgacgccct tgccgatgaa gggcaccagc accaccatca gcagcagcag cgccaccacg
     5581 aacagccagg gcgcggactt ttcccagacc tggatcggca gctgcaccgt gatggcgccc
     5641 gcgatcaggc cgatcgcgat gaacaaggcc tggcgcgtga ggaaatgcgt gggtgcgtag
     5701 tgcgcgaagc gcgggttgtc gggcagcgcg accgaggccg agtagaccat caccagcccc
     5761 agggcgagca gtgcgatgct cagccacagc agcgattggt cgaagcccag cagccgcgcc
     5821 ggctcgccgg acgagacgct gacccagtcg cgcaccggca cgctgccgtc gccgccggcg
     5881 cgcttcttct gcaggcggct ggcgaggagg ccgcgcagct gggccacaaa cccgaagcgt
     5941 gcggcggtgc tcacagcatc tccccacgct cggccgccag cgagcgcacc gtgtccttga
     6001 agacctcggc gcgatgggca tagttgcgga acatgtccag gctggcgcag gccgggctca
     6061 gcagcacggc atcgcccggc gcggcctgct cgaagcacca gcgcgtggcg gcctcgaggc
     6121 tgtcatgcct ttgcagcggc acgccggcag cgcccagggc cacgtccagg gcctcggcgt
     6181 cgcggccgat cagcgccgcc gcgcgaacgt gctgagagac gggctcgagc agcggcgaga
     6241 agtcctggcc cttgccttcg ccgcccagga tcacgagcag cttggccggt gccttgtcgg
     6301 cgcccaggcc gttcaaggcc gcgacagtgg cgccgacatt ggtgcccttg ctgtcgtcat
     6361 aggcctcgac ttcgcccacc ctgccgaggt actcgacgcg gtgcggctcg ccggcgtact
     6421 cgcgcaggcc atgcagcatc ggtgccagcg ggcagccgac ggataccgcc agcgccaatg
     6481 ccgccagcgc attggcagcg ttgtggcggc cgcgcacgcg cagcgcgtcg gccggcatca
     6541 ggcgctggat gtgcagctcc tcgacctcgt ccttgcggcg cttcacggtc tcgtccgctt
     6601 ccatcgcccg caccagccag gccatgccgt tctcgatgac gagtccgaaa tcgccagggt
     6661 gctgaggcgc atccaggccg aagcgcacca ccctgcgggt gatcggcttg gcgttgcggc
     6721 ccttcaccgg gatgggcgcc ggcaccatgg cctcgaccag cggatcgtcg cggttgacga
     6781 ccatcacggc ctgctggccg tagatgcgcg ccttggccgc ggcgtaggcc ggcatgtcgc
     6841 catgccagtc gagatggtcc tgcgtcacgt tgagcacggc cgctgcgctg ggctcgaagc
     6901 cctgcgcgcc gtcgagctgg aagctggaca actccagcac ccagacctcg ggcaggtgtt
     6961 cgaagcgcgg cgcggcgggt ggcggcgggg cgagctgcag cggaacagcg tcctcgtcct
     7021 cgggcggcgc ggcctcggct cccgtatcca cctcggcctc catcgcttct tgcgaggccg
     7081 aaggggcggg ggtcggcgca tccgaagagg gggcctcgac gctttccgcg ggggcagctt
     7141 cctgggcctg ctccaccggg acctcgggct ccagatccaa ggcctcggcc agggtctgca
     7201 gcatggtcgg gccgacattg cccgccacgg ccgcgcgctt gccggcacgg gcgaccaggg
     7261 cgccggccat catcgtggtg gtggtctt
//
LOCUS       DASUCY010000407         2363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_587411, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000407 DASUCY010000000
VERSION     DASUCY010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587411"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VFV25_08165"
     CDS             <1..252
                     /locus_tag="VFV25_08165"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HEY1393338.1"
                     /translation="NDERPIDESCSCHTCAGSGATGGVSRAYLHHLDRCGEMLGPMLT
                     SIHNLHYYVDLMREMREALDEGRFAEFAQRFAADRQRGV"
     gene            complement(262..708)
                     /locus_tag="VFV25_08170"
     CDS             complement(262..708)
                     /locus_tag="VFV25_08170"
                     /inference="COORDINATES: protein motif:HMM:NF021495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2214 family protein"
                     /protein_id="HEY1393339.1"
                     /translation="MDARTLDAVLAIVHHLLVFPLVAVLIAELALVHGTIDAPRLRQL
                     GRLDAAYGALAGGVLVAGLLRATFGAKGWSYYAGQPFFWAKLGLFMLIGMLSIPPTLR
                     LIHLRKGGSLPDAAALASLRGWMRTQAALLVWMPVLAVLMARSFGG"
     gene            782..1426
                     /locus_tag="VFV25_08175"
     CDS             782..1426
                     /locus_tag="VFV25_08175"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WHG domain-containing protein"
                     /protein_id="HEY1393340.1"
                     /translation="MAAKSALSDTYHHGALRAALLEAAEAILREGGLEAFTLRECARR
                     AGVSHAAPAHHFGDARGLLSACAAAGFERLSDLMQRYADREQGALERVRAAGVAYVDF
                     ALRNRALFQLMFRRDRLDPEHEELKAAGKRTGDLLRQIIDEVIVERGLAPQELGQRIL
                     LVWSLVHGYATLVLEDQCADLFGLDTAHTRAASRMGDELLRLLETGLAAPARRL"
     gene            complement(1536..>2363)
                     /locus_tag="VFV25_08180"
     CDS             complement(1536..>2363)
                     /locus_tag="VFV25_08180"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEY1393341.1"
                     /translation="EQLEGAPKGAGHGGPALVIRPEGAAQIALIGPPNAGKSSLHARL
                     TGSAAHVGPYPFTTRYPEAGMMPHEDVHFQLIDLPPIAPEHPVPWLVNTLQTADAALL
                     VVDLGEPSCLEQLQAVQSVLGQQRVTLTERQEAITGDDPFALRLPSLMLANKADGMVD
                     LDAEVLALRELSGLSLPVFAVSATTGHGLGDIGAWLFQNLGIVRVYTKTPGHSADRHR
                     PFTLRRGQTVGDVARLVHQDLARSLRYARVWGQSGFEGQQVGPDHCVADGDIVELHG"
BASE COUNT          395 a          808 c          799 g          361 t
ORIGIN      
        1 aacgacgagc ggccaatcga cgagagctgc agctgccaca cctgcgccgg ctcgggggcg
       61 acgggcggcg tcagccgcgc ctacctgcac cacctcgacc gctgtggcga gatgctgggg
      121 ccgatgctga ccagcatcca caacctgcac tactacgtgg acctgatgcg cgagatgcgc
      181 gaggcgctgg acgaaggccg cttcgccgaa ttcgcgcagc gcttcgcggc cgatcgccag
      241 cgcggcgtct gacctggggt ctcagccccc gaagctgcgt gccatcagca ctgccagtac
      301 cggcatccag accagcagcg ctgcctgagt acgcatccag ccgcgcaggc tcgccaatgc
      361 cgctgcgtcc ggcaggctgc cgcccttgcg caagtggatc agccgcagcg tgggcggtat
      421 cgacagcatg ccgatcagca tgaacagacc gagcttggcc cagaagaagg gttggccggc
      481 gtaatagctc cagcccttgg cgccgaaggt cgcgcgcaac agccccgcaa cgagcacgcc
      541 gccggccagg gcgccatagg ccgcatccag gcggccgagc tgtcgcagcc tgggtgcgtc
      601 gatggttcca tggacgagcg ccagctcggc gatgagcacg gccacgaggg ggaagaccag
      661 cagatggtgg acgatcgcca gcactgcatc gagggttcgg gcatccatat ctttacagtg
      721 tcaagttgag gtggggcaac tctaggtttg aatgttgacg ctgtcaagat aaaatcactg
      781 catggcagcc aaatcagctc tgtccgacac ctatcaccat ggcgcgctgc gtgccgcctt
      841 gctcgaagcg gccgaggcaa tcctgcgcga gggcggcctc gaggccttca cactgcgcga
      901 atgcgcgcgc cgcgcgggtg tctcgcatgc tgcgccggcg caccacttcg gcgatgcgcg
      961 cggcctgctc agtgcctgcg cggcggccgg cttcgagcgc ctgagcgatc tcatgcagcg
     1021 ctatgccgat cgcgagcagg gggcgctgga gcgcgtgcgt gccgccggcg tggcctatgt
     1081 ggacttcgcg ctgcgcaatc gcgccttgtt ccagctcatg ttccgccgcg accggctcga
     1141 tcccgagcac gaggaattga aggcggccgg caagcgcacc ggcgatctgc tgcgccagat
     1201 catcgacgaa gtgatcgtcg agcgcggcct ggcgccccag gagctcgggc agcgcatcct
     1261 gctcgtctgg tcgctcgtgc atggctatgc gacgctggtg ctcgaggacc aatgcgccga
     1321 cctattcggg ctcgacaccg cgcatacgcg cgcagcgagc cgcatgggcg acgagctgct
     1381 gcgcctgctg gaaaccggat tggccgcgcc ggcgcgtcgg ctctgagtat gtcggccgcc
     1441 gacataccgg cccctgacgg aagccggctt cgcgaagata tcgagcaagt gaatggcaac
     1501 gagtggcgaa cggcagggcc gagggctgac acggcttatc cgtgcagttc gacgatatcg
     1561 ccatccgcca cgcagtggtc gggcccgacc tgctgccctt cgaacccgga ctggccccag
     1621 acgcgcgcgt agcgcagcga gcgcgccaga tcctgatgca ccagccgcgc gacgtcgccc
     1681 acggtctggc cgcggcgaag cgtaaatggc cggtgcctgt cggcggaatg ccccggggtc
     1741 ttggtgtaga cgcgcacgat gccgaggttc tggaacagcc acgctccgat gtcccccagg
     1801 ccgtgacccg tggtcgccga aacggcgaac accggcaggc tcaggcctga cagctcgcgc
     1861 agtgcgagga cttccgcatc gaggtcgacc atgccatccg ccttgttcgc gagcatcaag
     1921 cttggcagcc gcagcgcgaa ggggtcatcg cccgtgatcg cctcctgacg ctcggtgagc
     1981 gtcacgcgct gctgccccag caccgactgc acggcctgca actgctcgag gcacgagggc
     2041 tcgcccaggt ccacgaccag cagcgccgca tcggctgtct gcaaggtgtt gaccagccat
     2101 ggcacgggat gctccggggc gatcggcggc aggtcgataa gctggaagtg cacatcctcg
     2161 tgcggcatca tccccgcttc cgggtaccgg gtcgtgaacg gatacggacc gacgtgcgcc
     2221 gcggaacccg tcagccgcgc gtgcagcgag gacttgccag cgttcggcgg cccgatcagc
     2281 gcgatctgcg ccgcgccttc ggggcggatt accagcgccg ggccgccgtg tcctgcgccc
     2341 ttcggggctc cttccagctg ttc
//
LOCUS       DASUCY010000408         2690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_619694, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000408 DASUCY010000000
VERSION     DASUCY010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619694"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="VFV25_08185"
     CDS             <1..676
                     /locus_tag="VFV25_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEY1393342.1"
                     /translation="DSAGGSAKMGRDKETQAILPLRGKVLNAWEVERDRLFANNEIHD
                     ISVAIGVDPHGASDEADLSGLRYGKICILSDADVDGSHIQVLLLTLFFRHFPRLIESG
                     HVYVAKPPLFRVDAPARGKKPAAKIYALDEGELVATLDKLRKEGARENSWTISRFKGL
                     GEMSAEQLWDTTLNPDTRRLLQVAYGLLDFGATEGSFNKLMGKGEAASRRELMELHGD
                     AVEVDV"
     gene            673..1026
                     /locus_tag="VFV25_08190"
     CDS             673..1026
                     /locus_tag="VFV25_08190"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY1393343.1"
                     /translation="MSLKIELRRIEAGPETEAAVQRVCEAAADYMLRISGLPPGKSYE
                     DKFTYGVFEGNKMVGCAELIRGWREPDSAHIGLLPLVPEARGRGTGGQAVAALEREAR
                     AWRAAARASLSRKAL"
     gene            1106..1339
                     /locus_tag="VFV25_08195"
     CDS             1106..1339
                     /locus_tag="VFV25_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEY1393344.1"
                     /translation="MQVSKWGNSLAVRLPAIVVELLGLKEGDDIEIEVAGERRFEVRR
                     SAAVQEVLQRLRAFRGRLPADFKFDRLEASERR"
     gene            1339..1806
                     /locus_tag="VFV25_08200"
     CDS             1339..1806
                     /locus_tag="VFV25_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012366058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEY1393345.1"
                     /translation="MAGLASRGSAPPFLDTNVLLYLLSADASKADRAEALLASGATIG
                     VQVLNEFAHVARRKLGMAWSEVEEVLGLIRQRCSVRPLKLAVHEQAMQLAQRHGFAWY
                     DALIVSAALDAKAPVLYSEDMHDGLEVASPDGAASLMISNPFTAPLREARWSP"
     gene            1806..2327
                     /locus_tag="VFV25_08205"
     CDS             1806..2327
                     /locus_tag="VFV25_08205"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY1393346.1"
                     /translation="MNRQVRLRPTMLSDLDYVQSVERDEHNQPFITPWERTQHEGAVR
                     FPDFRHFIVEAGAGTEPVGFVILQGCRNPNASVELKRIVLQTKGQGLGRACVRLLKQM
                     AFRDLKAHRFWLDVKTLNTRALALYASEGFVEEGRLRESVRVGDGWDSLVVMSMLDRE
                     YQARVALGEELSP"
     gene            2324..>2690
                     /locus_tag="VFV25_08210"
     CDS             2324..>2690
                     /locus_tag="VFV25_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEY1393347.1"
                     /translation="MTSAVKLLRICACALCLMSAGLPARAAVWGFVDGQGVPHFATSK
                     LDSRYEPMLDVGSENRVPGKSDGSSKIALWLEIAPDVKAVQHLLREAAQEEQVDVELL
                     KAMVAVESGFNAKAQSPRGA"
BASE COUNT          450 a          867 c          949 g          424 t
ORIGIN      
        1 cgattccgcc ggcggcagcg ccaagatggg ccgcgacaag gagacgcagg ccatcctgcc
       61 gctgcgcggc aaggtgctca acgcctggga ggtggaacgc gaccgattgt tcgccaacaa
      121 cgagatccac gacatctcgg tggcgatcgg cgtcgatccg catggcgcga gcgatgaagc
      181 cgatctctcc ggcctgcgct acggcaagat ctgcatcctc tccgatgccg acgtcgacgg
      241 ctcgcacatt caggtgctgc tgctcacgct cttcttccgc catttcccca ggctcatcga
      301 gagcggccat gtctacgtgg ccaagccgcc gctgttccgc gtcgacgcgc cggcgcgcgg
      361 caagaagccg gcggccaaga tctacgcgct ggacgaaggc gaactcgtcg ccacgctgga
      421 caagctgcgc aaagagggcg cgcgcgagaa ctcgtggacc atcagccgct tcaagggcct
      481 gggcgagatg agcgccgagc agctgtggga caccacgctg aaccccgata cgcgccgcct
      541 gctgcaggtg gcctacggcc tgctcgactt cggcgccacc gagggctcgt tcaacaagct
      601 gatgggcaag ggcgaggccg catcgcgccg cgagctgatg gagctgcacg gcgatgcggt
      661 ggaggtggat gtgtgagcct caagatcgag ctgcgccgca tcgaggcagg tccagaaacc
      721 gaggccgcgg tgcaacgcgt ctgcgaggcg gcggcggact acatgctgcg catctcgggc
      781 ctgccgcctg gcaagagcta cgaagacaag ttcacctacg gtgtcttcga aggcaacaag
      841 atggtgggct gcgccgaact gatccgcggc tggcgcgagc ccgacagtgc gcacatcggc
      901 ctgctgccgc tagtgcccga ggcgcgcggg cgcggcactg ggggacaggc tgtggccgcc
      961 ctggagcgcg aggctcgcgc atggcgcgca gcggcgagag caagtctttc gaggaaggcg
     1021 ttgtgaaatc cgtcgcgcat gtgatgttga agccggtgaa acagggttga tatacaatct
     1081 ttacgtatat cttctggagt gagccatgca agtgtccaag tggggcaaca gcctggcggt
     1141 gcgcctgccc gccatcgtgg tcgaattgct cggcctgaag gaaggcgacg acatcgagat
     1201 cgaagtggcc ggcgagcgcc gtttcgaagt gcggcgcagt gcggccgtac aggaagtgct
     1261 gcagcgcctg cgcgccttcc gcggccgcct gcccgccgac ttcaagttcg accggctcga
     1321 agccagcgag cgccgttgat ggccggcttg gcttccagag gctcggcgcc gcccttcctg
     1381 gacaccaacg tactgctcta cctgctctcg gcggatgctt ccaaggccga ccgcgccgag
     1441 gccctgctgg cctcgggcgc gaccatcggc gtgcaggtgc tcaacgagtt cgcccacgtg
     1501 gcgcggcgca agctgggcat ggcgtggagc gaggtcgagg aagtgctggg cctgatccgc
     1561 cagcgttgca gcgtgcgccc gctgaagctg gccgtgcacg agcaggcgat gcagctcgcg
     1621 caacgccatg gcttcgcttg gtacgacgcg ctgatcgtct cggccgcgct cgacgcgaag
     1681 gcgcccgtgc tttacagcga ggacatgcat gacggcctcg aagtggcgtc gccggacggc
     1741 gcggccagcc tgatgatcag caaccccttc acggcgcccc tgcgcgaagc gcgatggtcg
     1801 ccgtgatgaa ccgacaggtc cgcctgcgcc ccaccatgct gtccgatctg gactatgtgc
     1861 agtcggtcga gcgcgacgag cacaaccagc ctttcatcac gccctgggag cgtacccagc
     1921 acgagggcgc ggtgcgcttc ccggacttcc gtcacttcat cgtcgaggcc ggcgcgggca
     1981 cggagccggt gggcttcgtc atcctgcagg gctgccgcaa tcccaatgcc tctgtggagc
     2041 tcaagcgcat cgtgctgcag acgaagggcc agggcctggg ccgcgcctgt gtacggctgc
     2101 tcaagcagat ggccttccgc gacctgaagg cgcaccgctt ctggctcgac gtgaagacgc
     2161 tgaacacgcg cgcgctggcg ctgtacgcga gcgagggctt cgtcgaggag ggccgactgc
     2221 gcgagagcgt gcgcgtcggg gacggctggg actcgctggt cgtgatgagc atgctggacc
     2281 gcgaatacca ggcgcgcgtg gcgctgggcg aggagctgtc cccttgacct cggccgtgaa
     2341 gctcctgcgc atctgcgcct gcgcgctgtg cctgatgtcg gcgggcctgc cggcccgtgc
     2401 cgccgtctgg ggctttgtgg acgggcaggg cgtgccgcat ttcgccacca gcaagctgga
     2461 ttcgcgctac gaacccatgc tggatgtcgg cagcgagaac cgcgtgccgg gcaagagcga
     2521 cggctcgtcc aagatcgcgc tgtggctgga aatcgcgccc gacgtcaagg cagtgcagca
     2581 tctgctgcgc gaggccgccc aggaagagca ggtggacgtg gaactgctga aggcgatggt
     2641 ggcggtcgag tcgggcttca acgccaaggc gcagtcgccg cgcggtgccc
//
LOCUS       DASUCY010000409         2105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1470785, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000409 DASUCY010000000
VERSION     DASUCY010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1470785"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="VFV25_08215"
     CDS             <1..836
                     /locus_tag="VFV25_08215"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY1393348.1"
                     /translation="RAASLLGACLRTAYAIQPALGTRLAARLFFTPLPSKLAARRQRL
                     PSEWVPEAWPFERTSLTLYRRRDAGPGPVALLVHGWGGHGLQMQALGDGLAAAGLQPV
                     LLDFPAHGRSGGWSSTLPQFLRALEYVAARLGGVHSLVAHSLGALAAAAAQGRGLAAQ
                     KLVLLAPSPSPNQVTRWFAGSFGLDDDAHARMQSHIEAREGLLLDSFEPDRLAPRLAA
                     PILLIHDREDRMAPFANSERWAALAPQVTLSAVAGLGHRRLLADAGVIDQVRRWCTAK
                     K"
     gene            996..1253
                     /locus_tag="VFV25_08220"
     CDS             996..1253
                     /locus_tag="VFV25_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393349.1"
                     /translation="MSTPTRPARLRPAAPSCRGGASSLLRLTPCGNQGAPSLRGGERK
                     GAASQGLGAWLARAAGSLLPRGPAMPPAFGLSEFAVSRMPL"
     gene            1302..1496
                     /locus_tag="VFV25_08225"
     CDS             1302..1496
                     /locus_tag="VFV25_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393350.1"
                     /translation="MLLSLHRVEASQPEAPVSQPAPLSTIGGATPAPRQFKVLQSRGA
                     PGQRALPLTSLAATHSLRTP"
     gene            1779..>2105
                     /locus_tag="VFV25_08230"
     CDS             1779..>2105
                     /locus_tag="VFV25_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HEY1393351.1"
                     /translation="MSWFPQWRVKPAHGAPGAVVAPDERLPWPQTAAMGVQHVIAMFG
                     ATVLAPILMGLDPNVAILMSGIGTLIFFFIVGGRVPSYLGSSFAFIGVVIAASGYSGQ
                     GPNTHIG"
BASE COUNT          272 a          762 c          731 g          340 t
ORIGIN      
        1 gccgcgcggc gagcctgctg ggcgcctgcc tgcgcacggc ctatgccata cagccggcgc
       61 tgggcacgcg gctggccgcg aggctcttct tcacgccgct gccgagcaag ctggccgcgc
      121 ggcgccagcg cctgccttcg gagtgggtgc ccgaggcctg gcccttcgag cggacctcgc
      181 tgacgctgta tcgccgccgc gatgccggcc ccggccccgt tgccttgctg gtccacggct
      241 ggggcggaca cgggctgcag atgcaggccc tgggcgatgg cttggccgcg gccgggctgc
      301 agcccgtgct gctggatttc cccgcccatg gccgcagcgg cggctggagc agcacgctgc
      361 cgcagttcct gagagcgctg gaatatgtcg ccgcacgctt ggggggcgtg cacagcctgg
      421 ttgcacattc gctgggcgcg ctggcggcgg ccgctgccca ggggcgcggg ctggcggcgc
      481 aaaagctggt gctgctggca ccctcgccgt cgccgaacca ggtgacgcgc tggttcgccg
      541 gcagcttcgg cctggacgat gacgcccacg cgcgcatgca aagccacatc gaggcgcgcg
      601 aaggcttgct gctcgacagc ttcgagcccg atcggctggc accgcggctg gccgcgccta
      661 tcctgttgat ccacgatcgc gaggaccgca tggcgccctt cgccaacagc gagcgctggg
      721 ctgcgctggc gccccaggtg accctgagcg ccgtcgcggg cctgggccac cgtcggctgc
      781 tggccgatgc cggcgtgatc gaccaagtcc ggcgttggtg cactgcgaag aagtgactgt
      841 ttgcgtcagc ggcgtaacga ggtgcaaaag cctagcgccg ctttttgacg ttttcggggc
      901 cgcgggggcg gtacggatgg gtcagacccg gctccaggtg cctggcacgg aagctgcgct
      961 agccccagca accgaagcca gcagtatccg ccgccatgag caccccgacc cgccccgcac
     1021 gcctgcgtcc tgcagccccg agctgccgcg gcggcgcctc atccctgttg cgtctgacgc
     1081 cctgtggcaa ccagggcgcg cccagcctgc gcggcggcga gcgcaagggt gcggcgagcc
     1141 agggcctggg agcttggctg gcgcgtgccg ccggtagcct gttgccgcgc ggtccggcaa
     1201 tgccgcccgc cttcggcctg tccgagttcg cggtcagccg catgccgctc tgatagcgaa
     1261 ccgccagctt ttcgaacgcc ttagaagaga gaacgaacgc catgctgctg tccctccatc
     1321 gcgtcgaagc ctcccagccc gaggccccag tcagccagcc ggctcccctg tccaccatcg
     1381 gcggcgcgac gcccgcgccg cgccagttca aggtgctgca gtcgcgcggc gcgccgggcc
     1441 agcgtgccct gccgctgacg agcctggcag ccactcacag tttgcggacc ccttgacgtc
     1501 cagggggagc gggcacccgg ggtaggagag agcgagcccc cggtccctgt cccgctcccc
     1561 tgtctttttt ctccgcagac cttcttcctt ctccgttttc cgtttcgccc gggctgccgt
     1621 gtttgcggca gccgctgaat cgggcatcgc tgtgcggttt taccgcagct tgcccgacat
     1681 agtgccggcc ccggtgacgg tatgcgccgg gctgcctaaa ctgcgccggg gcgcccgccg
     1741 ggccggccga aaccgaaagc cgcgagagga gaagagcaat gagttggttc ccgcaatggc
     1801 gcgtcaagcc cgcccatggt gcgcccggcg ccgtggtcgc gccggacgag cgcctgccgt
     1861 ggccgcagac cgccgcgatg ggcgtccagc atgtgatcgc gatgttcggc gcaacggtgc
     1921 tggcgccgat cctgatgggc ctggatccca atgtggcgat cctgatgtcg ggcatcggca
     1981 ccctgatctt cttcttcatc gtcggcggcc gggtgccgag ctacctgggc tccagcttcg
     2041 ccttcatcgg cgtcgtgatc gccgccagcg gctattcggg ccagggcccc aacacccata
     2101 tcggc
//
LOCUS       DASUCY010000410         2002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_828521, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000410 DASUCY010000000
VERSION     DASUCY010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828521"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /gene="bamC"
                     /locus_tag="VFV25_08235"
     CDS             <1..962
                     /gene="bamC"
                     /locus_tag="VFV25_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HEY1393352.1"
                     /translation="RYQPASGTVNASSFQSGAAAAGAAAPAAGQAVAPRAVGDMHIER
                     DGNQRWLVTPLAADALWPQLQQFWKDQGLTLVTDDAATGVMETDWAENRANLPKDIIR
                     STIGRVFDSLYDTGKRDRFRTRVERTATGTEIYISHRGMVEVVSSQQTGQTVWQPSPP
                     DPGLEAEMLSRLMLRLGAKEEAAKASAAAVAEAAAAPVGPAKVRVLTDRPYAAFEIDD
                     GFDRAWRRVGLSLDRSGFTVEDRDRAQGLYYVRYVDPNSVKEPNFFTKLFSKDGNNAP
                     ARYRLSLKSQGELTTVAVLNAQGAPESGDAAKHIVKLLVEDLK"
     gene            990..1769
                     /locus_tag="VFV25_08240"
     CDS             990..1769
                     /locus_tag="VFV25_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY1393353.1"
                     /translation="MRFCSLGSGSSGNATLIEAGDGARRSRVLVDCGFSIKELAARLA
                     RAGLEPAELDAIFVTHEHADHIGSAIPLAKRYGLPLWMSQGTWRAVGAPELPSGLLDF
                     ARDGERIAIGALELAPYTVPHDAQEPLQLALGDGAARLGILTDAGSNTGHLLAALAGC
                     QALLLECNHDRELLANSRYPPALKARIAGRHGHLANDTSAAILAGVMHAGLKHVVAAH
                     LSEQNNRPALARAALATVLGAACEDIVVADPRRGFAWLDVS"
BASE COUNT          339 a          687 c          672 g          304 t
ORIGIN      
        1 cgcgctatca accggcgagc ggtacggtga atgccagcag cttccagtcc ggggcggctg
       61 ccgccggagc tgctgcgccc gcggctggcc aggctgtggc gccgcgcgcc gttggcgaca
      121 tgcacatcga gcgggacggc aatcagcgct ggctcgtcac gcctctggcg gccgatgcgc
      181 tgtggcctca gctgcagcag ttctggaagg atcagggcct gaccctcgtc accgacgacg
      241 ccgccacggg cgtgatggaa accgactggg cagagaaccg cgccaatctg cccaaggaca
      301 tcatccgcag caccatcggc cgggtgttcg attcgctcta cgacaccggc aagcgcgacc
      361 gcttccgcac ccgggtcgag cgcacggcaa ccggcactga gatctacatc tcgcatcggg
      421 ggatggtcga ggtcgtcagc tcgcagcaga ccggccagac ggtctggcag ccgagcccgc
      481 ccgatcccgg cctagaggca gagatgctct cgcgcctgat gctcaggcta ggtgccaagg
      541 aagaggccgc caaggccagc gcggccgcgg tggcggaagc agctgcggct ccggtgggac
      601 ccgccaaggt gcgcgtgctg accgatcggc cttatgccgc cttcgagatc gacgacggct
      661 tcgatcgcgc ctggcgccgc gtcggcctgt cgctggatcg cagcggcttc acggtggaag
      721 accgcgatcg ggcgcaggga ctgtattacg tgcgctatgt cgacccgaac agcgtgaagg
      781 agcccaattt cttcaccaag ctcttcagca aggatggcaa caacgccccg gcgcgctacc
      841 gcctgtcgct gaagtcgcag ggcgagctca ccacggtggc ggtgctgaat gcgcaaggcg
      901 cgccagagtc cggcgacgcg gccaaacaca tcgtgaagct gctggtcgaa gacctcaagt
      961 aggcgcgggc gccgcaagga gcaggcgtgc tgcgcttctg cagcctgggt agcggcagca
     1021 gcggcaacgc gacgttgatc gaggctggcg acggagctcg ccgcagccgc gtgctagtcg
     1081 attgcggctt ctcgatcaag gagctcgccg cgcgtctggc ccgcgccggg ctcgaacctg
     1141 ccgaactgga cgccatcttc gtcacccacg agcatgccga ccatatcggc agcgccattc
     1201 ccttggccaa gcgctacggc ttgccgctgt ggatgagcca aggcacctgg cgagccgttg
     1261 gcgcgcctga actgccctcc ggcttgctgg actttgcccg cgacggcgag cgcatcgcca
     1321 tcggcgcatt ggaactcgcg ccctacaccg tgccgcatga tgctcaagaa ccactgcagc
     1381 tcgcgctcgg cgacggagcc gcccgcctgg gcatcctgac cgatgcgggc agcaacaccg
     1441 ggcatctgct cgcagcgctg gccggctgcc aggctctgct gctggaatgc aaccacgacc
     1501 gcgagctgct ggcgaactcg cgctacccac ccgctctgaa ggcgcgcatc gcgggccgcc
     1561 acggacatct ggcgaacgac acctcggcgg caatcctggc cggggtcatg catgccggcc
     1621 tgaagcatgt ggtggcggcg cacttgagcg aacagaacaa ccgacccgcg ctggctcgcg
     1681 cggcgctggc gacggtgctg ggcgcggctt gcgaggatat cgttgttgcc gatcccaggc
     1741 gcggattcgc ctggctggac gtcagctgaa agaaccggcc gcatcaaaca atcgggatag
     1801 gggcggccag attatctgac cgacccctcc cacaccaccc ggcatgcggg tccgcaccgg
     1861 gcggttcgag agcttgaggt ccttgacttc gcttgcggca ggccgccagg gcccgccatc
     1921 ggctccatca accctgtacc tcggtattta caaggcgggc cgtcggcttc gtcgctgaag
     1981 ggatcgctcg cggcttcacc gc
//
LOCUS       DASUCY010000411         4377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_507247, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000411 DASUCY010000000
VERSION     DASUCY010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507247"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VFV25_08245"
     CDS             complement(<1..214)
                     /locus_tag="VFV25_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HEY1393354.1"
                     /translation="MRIVMLAGTAAALAPASAFAVDYLSAEQAARLMFPEADQFESRA
                     VDLDAAELQRFEATTGVRARSAHWPLR"
     gene            complement(217..1548)
                     /locus_tag="VFV25_08250"
     CDS             complement(217..1548)
                     /locus_tag="VFV25_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HEY1393355.1"
                     /translation="MQASSPLRLSVAAALVSAAFAGPAYGQTTQEAELLQRVDKLAAE
                     LQALKAQLAQLQSERQAERQRSAEQPVPATASAAAPPAPAPAPAAAAASSEPATVLTG
                     YGEINYNRTTKHSENSQADLRRFVLGYQHRFDEKTKLVTELEVEHAIASSSDQGEAEI
                     EQAFIDHQINPTWSLRGGLMLMPAGLLNENHEPTAYYGVERNFVETAIIPSTWREGGV
                     QAVANLDSGLTLQGGLSTGFNLGKWDATSTDGAASPLGSIHQEMQLAKAHDIAAFGAA
                     NWRGVPGLLLGASLFSGGATQGEPGKPSSRVTLWDLHARWTPGAWDLSGVYARGGISN
                     TAALNKPLAGSATLIPETFYGWYAQAAYKLWSSGDYSLSPFVRYERYNTGSAYADIGA
                     GLTPNPRPTERVVTLGANFKLNSSVVIKADVQRFKEASDNDRFDLGLGWSF"
     gene            complement(1619..2773)
                     /locus_tag="VFV25_08255"
     CDS             complement(1619..2773)
                     /locus_tag="VFV25_08255"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEY1393356.1"
                     /translation="MLFALLSACGGGGSASEPAPGKLSASAALGEKIFKDASLSASGR
                     QSCASCHDPAAVHAPANNLAAQFGGPNMDRQGLRQSPSIRYLSFNTPFHFAADGTPTG
                     GFFWDGRADTLALQAGQPFLNPREMANADKAAVVAKLARASYAAEFKQLYGEAVFSDV
                     EGAFARMTLALQQYQREDNEFHSFSSKYDAFLQGKASLSDQELRGLVLFNSPTKGNCF
                     SCHPSARSADGTPPLFTDFTYDNLGVPRNPELAANDDPSHYDRGLCARDGGDLASRTD
                     LCGAFKVPSLRNVAQRQTFFHNGRFKTLKEALTFYVQRDTNPDKWYPLNADGTVKKFD
                     DLPLQYHANVNTKEVPYNRRPGDAPALSDAEIGDLIAFLKTLSDGYTANP"
     gene            3016..4320
                     /gene="serS"
                     /locus_tag="VFV25_08260"
     CDS             3016..4320
                     /gene="serS"
                     /locus_tag="VFV25_08260"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEY1393357.1"
                     /translation="MLDITLLRKDLEGVVARLNQRKNPQPYLDVERFSALESERKFIQ
                     TRTEELQAKRNSLSKQIGQLKAKGQDTAAVMAEVGGLGDELKASAERLEVIQAELSQM
                     LMSVPNLPHESVPVGDDETANAEVRRWGTPKSFGFAPRDHVDLGAPLGLDFETGAKLS
                     GARFTFMRGPVARLHRALAQFMLDVQTQEHGYTECYTPYIVNREVLEGTGQLPKFKED
                     MFWVFRGSEGDDDEQAEQYLISTSEISLTNTVRGQILAAEQLPIKLTAHSPCFRSEAG
                     SAGRDTRGMIRQHQFDKVEMVQIVHPEKSYEALEQMVGHAEAVLQKLELPYRVITLCT
                     GDMGFGAAKTYDLEVWLPAQNTYREISSCSNCEAFQARRMHARFRNAQGKPELVHTLN
                     GSGLAVGRTLVAVLENCQQADGSIAVPAVLRPYMGGLEVLRA"
BASE COUNT          743 a         1396 c         1507 g          731 t
ORIGIN      
        1 cacgcagcgg ccagtgcgcc gagcgcgcgc gcacgcccgt ggtcgcctcg aagcgctgca
       61 gctcggcggc atcgagatcg acggcgcgcg actcgaactg atcggcctcg ggaaacatca
      121 gcctggctgc ctgctcggca ctcaggtaat ccaccgcgaa ggcggacgcc ggcgccagcg
      181 cggcggccgt tccggccagc atgacgatgc gcatggtcag aagctccacc caagccccag
      241 gtcgaagcgg tcgttgtcgc tcgcctcctt gaagcgctgc acatcggcct tgatcacgac
      301 gctgctgttg agcttgaagt tggcgcccag cgtgacgacg cgctcggtcg gacggggatt
      361 cggcgtcaag ccagcaccga tatctgcata ggcgctaccc gtgttgtagc gctcgtagcg
      421 gacgaagggc gagaggctgt agtcgcccga ggaccagagc ttgtaggcgg cctgggcata
      481 ccagccatag aaggtctcgg ggatcagcgt cgcgctgccc gccagtggct tgttcagcgc
      541 ggccgtgttg ctgatgccgc cgcgcgcata gacgccggag agatcccagg cgccgggcgt
      601 ccagcgtgca tgcagatccc acagcgtgac gcgcgacgaa ggcttgcccg gctcgccctg
      661 cgtggcgccg ccgctgaaga gcgaagcgcc cagcagcagc cccggcacgc cgcgccagtt
      721 ggccgcgccg aaggcggcga tgtcatgcgc cttggcaagc tgcatttcct gatggatgga
      781 gccgagcggc gacgcggcgc catcggtcga ggtggcgtcc catttgccga gattgaagcc
      841 cgtgctcaag ccgccctgca acgtgaggcc gctgtcgaga ttggcgactg cctgcacgcc
      901 gccttcgcgc caggtgctgg ggatgatggc ggtctcgacg aagttgcgct ccacgccgta
      961 gtaggcggtc ggctcgtggt tctcgttgag cagccccgcc ggcatcagca tcagaccgcc
     1021 gcgcagcgac caggtggggt tgatctgatg gtcgatgaag gcctgctcga tctccgcctc
     1081 gccctggtcg ctggacgacg cgatggcgtg ttcgacctcc agctcggtga cgagcttggt
     1141 cttctcgtcg aagcgatgct ggtagccgag cacgaagcga cgcaggtcgg cctggctgtt
     1201 ctcgctgtgc ttggtggtgc ggttgtagtt gatctcgcca tagccggtga gcaccgtggc
     1261 gggctcggac gaggcggccg ctgcggcagg cgcaggcgca ggggctggcg gcgctgccgc
     1321 gctcgcggtt gccggcacag gctgctcggc actgcgctgg cgctcggctt gccgctcgct
     1381 ctgcaattgg gcgagttggg ccttgagggc ctgcagttcg gcagccagct tgtcgacgcg
     1441 ctgcagcagc tcggcttcct gcgtagtttg gccgtaggcg gggccggcga aggcggcgct
     1501 caccaaggcg gcggcgacgc tcaggcgcag gggggacgat gcttgcatgt tcgagttctc
     1561 cattcgaggt tgttcaagag ataaagaaaa gaagtagtgg tccgcgagct gggcggcttc
     1621 agggattcgc cgtgtagccg tcgctcagcg tcttcaggaa ggcgatcaga tcgccgatct
     1681 cggcgtcgct cagcgccggc gcgtcgccgg ggcggcggtt gtagggcact tccttggtgt
     1741 tgacgttggc gtggtactga agcggcaggt cgtcgaactt cttgaccgtg ccgtcggcgt
     1801 tgagcgggta ccacttatcg gggttggtgt cgcgctgcac atagaaggtg agcgcctcct
     1861 tcagcgtctt gaagcggccg ttgtggaaga aggtctggcg ttgcgctacg ttgcgcagcg
     1921 agggcacctt gaaggcgccg cacagatcgg tgcgcgaggc caggtcgccg ccgtcccggg
     1981 cgcacagccc gcggtcgtag tggctcgggt cgtcattggc ggccagttct ggattgcgcg
     2041 gtacgccgag gttgtcgtag gtgaaatcgg tgaagagcgg cggcgtgccg tcggcactgc
     2101 gcgcggaagg atggcagctg aagcaattgc ctttggtcgg tgaattgaac agcacaaggc
     2161 cgcgcagctc ctggtcgctc aggctggcct tgccctgcaa aaaggcgtcg tacttgctgc
     2221 tgaaggagtg gaactcgttg tcctcgcgct gatactgctg cagcgccagc gtcatgcgcg
     2281 caaaggcgcc ctcgacatcg ctgaagacgg cttcaccata aagctgcttg aactcggcgg
     2341 cgtagctggc ccgggccagc ttggcgacca cggcggcctt gtcggcattg gccatctcgc
     2401 gcggattcag gaagggctgg cccgcctgca gcgccagggt atcggcgcgt ccgtcccaga
     2461 agaagccacc ggtcggggtg ccgtcggcgg cgaaatggaa gggcgtgttg aagctcaggt
     2521 agcggatcga cggcgactgg cgcagcccct gccggtccat gttcggcccg ccgaactggg
     2581 ccgccagatt gttggccggc gcatgcacgg cagccgggtc gtggcagctc gcacaggact
     2641 gccggccgga cgcggacagt gacgcgtcct tgaagatctt ttcgcccaac gccgcactgg
     2701 ccgagagctt gccgggcgca ggctcggaag cgctcccacc tcctccgcag gcggacagga
     2761 gcgcaaacag caagagcaag gcagcacgag cggccgacgc ggaccaaaca gaacaacgca
     2821 aaacgcgccc ctccctgatc gataaaaatg caaacgagaa tgattatcat atagattgac
     2881 tagcgaacct gacaagtccg tcctttccgt cgggtcgctc gcgggcgtgt tgtcgcgagc
     2941 cgggtcggag cgagtcgcct cctacaatcg ccgcttcact gctgaccttt gcgcgcccgc
     3001 cgcggcgcgt tcgccatgct cgacatcacc ctgctgcgca aggatctgga aggcgttgtc
     3061 gcgcgcctga accagcgcaa gaacccccag ccctatctcg acgtggagcg cttctcggcg
     3121 ctggagtccg agcgcaagtt cattcagacc cgcaccgagg aactgcaggc caaacgcaac
     3181 agcctgtcca agcagatcgg ccagctcaag gccaagggcc aggacacggc ggcggtgatg
     3241 gccgaggtcg gtgggttggg tgacgaactc aaggccagcg ccgagcgcct tgaggtgatc
     3301 caggccgagc tgagccagat gctgatgtcg gtgcccaacc tgccgcacga gagcgtgcct
     3361 gtgggcgacg atgagaccgc caatgccgaa gtgcgccgct ggggcacgcc caagagcttc
     3421 ggcttcgcgc cccgcgatca cgtggacctc ggcgcgccgc tgggcctgga cttcgagacc
     3481 ggcgccaagc tctcgggcgc acgcttcacc ttcatgcgcg ggccggtcgc ccggctgcat
     3541 cgcgcgctcg cgcaattcat gctcgacgtg cagacgcagg agcacggcta caccgagtgc
     3601 tacacgccct acatcgtcaa ccgcgaagtg ctcgaaggca cggggcagct gcccaagttc
     3661 aaggaggaca tgttctgggt cttccgtggg agcgagggcg acgacgatga gcaggccgag
     3721 caatacctga tctcgacttc ggagatctcg ctaaccaaca ccgtgcgcgg gcagatcctg
     3781 gcggccgaac agctgccgat caagctcact gcgcacagcc cttgcttccg ctcggaggcg
     3841 ggcagcgctg gccgcgacac gcgcggcatg atccgccagc accagttcga caaggtcgag
     3901 atggtgcaga tcgtgcatcc cgagaaaagc tatgaggcgc tggagcagat ggtcgggcac
     3961 gccgaggccg tcttgcagaa gctggagctg ccctatcgcg tgatcacgct gtgcaccggc
     4021 gatatgggct tcggcgccgc caagacctat gacctcgaag tctggctgcc ggcgcagaac
     4081 acctatcgcg agatcagctc ctgctccaac tgcgaggcct tccaggcgcg gcgcatgcat
     4141 gcgcgcttcc gcaacgccca gggcaagccc gaactcgtgc acacgctcaa cggctccggc
     4201 ctcgccgtgg gccgcaccct ggtggcagtg ctcgaaaact gccagcaggc cgacggcagc
     4261 atcgccgtgc ctgccgtgct gcgcccatac atgggcggcc tcgaagtctt gcgggcctga
     4321 tccgctacaa tctccggctc cgcttctcac agcgagacca cgattgcagg aaaggtg
//
LOCUS       DASUCY010000412         3552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_764413, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000412 DASUCY010000000
VERSION     DASUCY010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764413"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1672)
                     /locus_tag="VFV25_08265"
     CDS             complement(<1..1672)
                     /locus_tag="VFV25_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEY1393358.1"
                     /translation="MSALVQPRLPFPYRVEAPLVQGLLDGLDGKLDWAQAADAAAPWV
                     QAVRDKPAPFWALESLLREYPISSAEGLALMRLAEALLRVPDAETAIALTADQLGRAE
                     FDGAGNAGPHKMLASLSANAIALSKKFLPEGDHPPGLLKRLGAQTVVAATVRAIQLLG
                     RQFVLGQSIQEALGEAHGQRKQQPNLRFSFDMLGEGARSEADAKRYLAAYQHALQAIA
                     AGGRAASPQEADGISIKLSALFSRYEDAQVERVRAELLPRVRTLIDAAARANLNLTID
                     AEESERLELSLEVFEALADHIAAEHPQWSGFGLAVQAYQTRALQVVHEVARIAKARGL
                     RFMVRLVKGAYWDGEIKRAQELGLPGYPVFTRKHHTDIAYLACALALLEHAPHIYPQF
                     ATHNAGTIAAILEMARALGADFEMQRLHGMGESVYREVLKDASIACRVYAPVGQHRDL
                     LAYLVRRLLENGANSSFVHQLADESVGLEELLISPLHLSAEPGQPAPAGLYGAQRANS
                     RGADLACASMREPLLAALADTRVPDVEEATPGAAAAAMEALATAWPSWQ"
     gene            1767..2252
                     /locus_tag="VFV25_08270"
     CDS             1767..2252
                     /locus_tag="VFV25_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEY1393359.1"
                     /translation="MSSAAEDTNKGLDKIDRKILRVLQEDGRISNLKLAEAVHLSPTA
                     VLERVKRLTREGYILGYEAKLNPVKLGAGLLVFVEVLLDRTVHDVMDTFKAAVQVRPE
                     ILECHLVAGGFDYLLKTRVADMTAYREFIGSVIWSLPGVRETRTYAVMEEVKNSSALK
                     L"
     gene            complement(2256..2945)
                     /locus_tag="VFV25_08275"
     CDS             complement(2256..2945)
                     /locus_tag="VFV25_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEY1393360.1"
                     /translation="MTTIAANLQQVQARIAASCAAAQRPASSVTLLAVSKTQHAAAVR
                     EAHAAGQRAFGENYVQEALDKIAALADLRAQLEWHLIGPLQSNKTRAVAESFDWVHSV
                     DRLKLAQRLAEQRPAALPPLNVCLQVNISGEPSKSGLAPDEVSELAHQVAALPRLRLR
                     GLMAIPEPAGDFEAQRAPHRRLCELLERLRGEGLALDSLSMGMSADLEAAIAEGATIV
                     RVGTAIFGMRP"
     gene            3002..>3552
                     /locus_tag="VFV25_08280"
     CDS             3002..>3552
                     /locus_tag="VFV25_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEY1393361.1"
                     /translation="MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDPLEH
                     KQVHDMVYDIMNDAQRKAYEETLECDFSFEIQGLARFRVNAFNQNRGAGAVFRTIPSK
                     ILTLEQLNAPKVFGELALKPRGLVLVTGPTGSDKSTTLAGMVNHLNENEYGHVLTVED
                     PIEFVHESKKCLVNQREVGPHTL"
BASE COUNT          576 a         1199 c         1213 g          564 t
ORIGIN      
        1 cctgccagct cggccatgcc gtggccagcg cttccatcgc cgccgcggcg gcgccgggcg
       61 tcgcctcttc gacgtcgggc acgcgagtgt cggcgagggc cgcgagcagg ggttcgcgca
      121 tgcttgcgca ggcgagatcc gcgccgcgcg aattggcgcg ctgcgcgcca tagaggccgg
      181 ccggcgcggg ctggccgggc tcggcgctca ggtgcagcgg cgagatcagc agctcctcca
      241 ggcctacgct ctcgtcggcg agctggtgca cgaaggagga gttggcgccg ttctccagca
      301 ggcgtcgcac caggtaggcg agcaggtcgc gatgctggcc gaccggtgcg tagacgcggc
      361 aggcgatgct cgcgtccttc aacacctcgc ggtacacgct ctcgcccatg ccgtgcaggc
      421 gctgcatctc gaagtctgcg cccagcgcgc gcgccatctc caggatggcc gcgatcgtgc
      481 ccgcgttatg ggttgcgaac tgcgggtaga tgtgaggcgc gtgttccagc aaggccagcg
      541 cgcaggcgag gtaggcgatg tcggtgtggt gcttgcgcgt gaagaccgga tagcccggca
      601 ggcccagctc ctgggcgcgc ttgatctcgc cgtcccagta ggcgcccttg accaggcgca
      661 ccatgaagcg cagaccgcgt gccttggcga tgcgcgccac ttcgtgcacg acctgcagcg
      721 cgcgggtctg gtaggcctgc accgcgaggc cgaaaccgct ccattgcgga tgctcggcgg
      781 cgatgtgatc ggccagcgcc tcgaagactt ccagcgagag ctccagccgc tcgctctcct
      841 cggcgtcgat ggtgagattg agattggcgc gcgccgccgc atcgatcagg gtccgcaccc
      901 gcggcaacag ctcggcgcgc acgcgctcga cctgcgcgtc ctcatagcgc gagaacagcg
      961 ccgacagctt gatcgagatg ccgtccgcct cttgcgggct ggcggcgcgg ccgccagccg
     1021 cgatggcctg cagcgcgtgc tggtaggcgg cgagatagcg cttcgcatcg gcctcgctgc
     1081 gggcgccctc gcccagcatg tcgaagctga agcgcagatt gggctgctgc ttgcgctggc
     1141 cgtgcgcctc gcccagggcc tcctggatgc tctggccgag cacgaactgt cgacccagca
     1201 gctggatcgc gcgcaccgtc gcagcgacca cggtctgcgc gcccaggcgc ttgagcagcc
     1261 ccggcggatg gtcgccttcg ggcaggaact tcttcgacaa cgcgatcgcg ttggccgaca
     1321 gcgaggccag catcttgtgc gggccggcgt tgccggcgcc gtcgaattcg gcgcgtccga
     1381 gctggtcggc ggtgagcgcg atcgccgttt cggcatcggg cacgcgcagc agggcctcgg
     1441 ccaggcgcat cagggccagg ccttcggcgc tggagatcgg gtattcgcgc agcagcgatt
     1501 ccagcgccca gaacggcgcc ggcttgtcgc gcacggcctg cacccagggg gcggccgcat
     1561 cggcggcctg ggcccagtcc agcttgccgt ccaggccgtc gagcaggccc tgcacgagcg
     1621 gcgcttctac gcggtaggga aagggcaggc ggggctggac gagggcagac atggcagggc
     1681 tcggtggggc tagggattga atctatggct aggttaagca gttgtcccta gcatgaatca
     1741 ccaaagaagc accatccagt agataaatgt catccgccgc cgaagacacc aacaagggat
     1801 tggacaagat agaccgcaag attctgcgcg tgctgcagga ggatggacgc atttccaatc
     1861 tcaagctcgc cgaggccgtg cacctatcgc ccaccgccgt gctggagcgc gtgaagaggc
     1921 tgacccgcga gggctatatc ctgggctacg aggcgaagct gaacccggtc aagttgggcg
     1981 ccggactgct ggtattcgtc gaggtgctgc tggaccgcac cgtgcacgac gtgatggaca
     2041 ccttcaaagc cgccgtgcag gtacgccccg agatcctcga atgccacctc gtggccggcg
     2101 gcttcgacta cctgctcaag acccgcgtgg ccgacatgac ggcctatcgc gagttcatcg
     2161 gcagcgtgat ctggagcctg cccggggtgc gcgagacgcg cacctatgcg gtgatggaag
     2221 aggtgaagaa cagctctgcc ctcaagcttt gaggctcagg gccgcattcc gaagatcgcc
     2281 gtgcccacgc gcacgatggt cgcgccttcg gcgatcgccg cctccaggtc ggcgctcatc
     2341 cccatcgaca agctgtccag cgccaggccc tcgccacgca ggcgctcgag cagctcgcac
     2401 agccggcgat gcggtgcgcg ctgagcctcg aagtcgccgg cgggctcggg gatggccatc
     2461 aggccgcgca ggcgcagccg cggcaatgcg gccacctgat gagccagttc gctcacctca
     2521 tcgggcgcga ggccgctctt gctcggctcg ccgctgatgt tgacctgcag gcagacgttg
     2581 aggggcggca aagccgcggg gcgctgctcg gccaggcgct gggcgagctt gaggcggtcg
     2641 acgctgtgca cccagtcgaa gctctcggcg acggcgcggg tcttgttgct ctgcaagggc
     2701 ccgatcagat gccactcgag ctgggcgcgc agatcggcga gcgccgcgat cttgtccagg
     2761 gcttcctgca cgtagttctc gccgaaggcg cgctggcctg ccgcatgcgc ctcgcgcacg
     2821 gcggcagcgt gttgcgtctt gctcaccgcc agcaacgtga cgctactcgc gggccgttgc
     2881 gccgcggcac agctcgcggc gatgcgcgct tgcacttgtt gtaagttcgc agcaatcgtc
     2941 gtcataatgc cccgagctta agcgagcatt gcgcgctcgc gctcctcgca tcccccgaca
     3001 catggacatc actcaactgc tggcgttctc ggtcaagaac aaggcttccg acctccacct
     3061 gtccgccggc ttgccgccga tgatccgcgt gcatggcgac gtgcgccgca tcaacgtgga
     3121 cccgctagag cacaagcagg tgcacgacat ggtgtacgac atcatgaacg atgcccagcg
     3181 caaggcctac gaagagacgc tggagtgcga cttctcgttc gagatccaag gcctcgcgcg
     3241 ctttcgcgtc aacgccttca accagaaccg cggcgcgggc gccgtgttcc gcacgattcc
     3301 ctcgaagatc ctcacgctgg agcagctcaa cgcgcccaag gtcttcggcg aactcgcgct
     3361 caagccgcgc ggcctcgtgc tcgtcaccgg ccccacgggc tcggacaaat ccaccacgct
     3421 ggcgggcatg gtgaaccacc tcaacgagaa cgagtacggc cacgtgctga cggtcgaaga
     3481 cccgatcgag ttcgtgcacg agtcgaagaa gtgcctggtc aaccagcgcg aggtcggccc
     3541 gcatacgctg ag
//
LOCUS       DASUCY010000413         2113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_186148, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000413 DASUCY010000000
VERSION     DASUCY010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186148"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="VFV25_08285"
     CDS             <1..609
                     /locus_tag="VFV25_08285"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEY1393362.1"
                     /translation="QDGQVWLLDEHLQRLDAACRHFGYAPLAAEGIAELRALRHSHAR
                     GAYKLRLTLSRQGAAHIEAQPLADTPVPLRVALAREPIASSDSEFLRHKTTRRELYAP
                     FAPEAGCFDTLLWNARGELTEFVNGNLALCLDGRWLTPALDAGLLPGTYRARLLAGGG
                     LQTARLVLDDLRRAGAAAFFNSVRGWLAVDLRSLMDRLPPQE"
     gene            642..1034
                     /locus_tag="VFV25_08290"
     CDS             642..1034
                     /locus_tag="VFV25_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393363.1"
                     /translation="MTPWHQYWTHWMELAREAPEEVARRADLITRAPGSPLSLADSQR
                     VLWENWTAATESWWSWWKAAHTMQWPTMPWPPAGVVPPPEEAPAAPAPAPAAAAPAPK
                     GKRVSAAPAAPAAPDPALKARVRRPRHR"
     gene            complement(1136..>2113)
                     /locus_tag="VFV25_08295"
     CDS             complement(1136..>2113)
                     /locus_tag="VFV25_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014617827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY1393364.1"
                     /translation="STTPETVVDNNRLSGHLYGVYLQDEWKPTPALTVNYGARYDQVD
                     TVVKESQLSPRLGATWQLGENTRLHAGYARYFTPPASEKIDLTSVQKFQGTTNALPSD
                     ANTAVKSERTNYFDAGISHQLTPQLTLGADAYYRDVRHLQDEGQFGNALIFSNFNYER
                     GRIYGLELSASYRHQNFSAYGNLAFSSAKGRNIETGQFNFGSDELAYIADHWVHLDHD
                     QVVAASMGAAYRFADGTALSSDLIYGSGLRRGFVNSEHLPSYTQVNAAVARSFDLGAG
                     FGRLDARFSVINVFDRVYELRDGTGIGVSAPQFGPRRGFYVSLSKPFSF"
BASE COUNT          328 a          727 c          723 g          335 t
ORIGIN      
        1 caggatggcc aagtctggct gctggacgag cacctgcagc ggctggacgc ggcctgccgc
       61 catttcggct acgcgccgct cgcggcggaa ggcattgccg agctgcgcgc gctgcggcat
      121 tcgcacgcga ggggtgccta caagctcagg ctaaccctgt cccgccaggg cgcggcgcat
      181 atcgaagccc agccgctggc cgacacgccc gtgccgctgc gcgtcgcact ggcgcgcgaa
      241 cccattgcaa gcagcgacag cgaattcctg cgccacaaga ccacgcggcg cgagctgtac
      301 gcccccttcg cgcccgaggc cggctgcttc gacacgctgc tgtggaacgc acgcggcgag
      361 ctcaccgagt tcgtcaacgg caatctcgcg ctctgccttg acgggcgctg gctcacgccc
      421 gcgctggatg ccggcctgct gcccggcacc tatcgcgccc ggctgctggc cggcggcggg
      481 ctgcagaccg cgaggctggt gctggacgat ctgcgccgcg ccggcgccgc cgccttcttc
      541 aatagcgtgc gcggctggct ggccgtcgat ttgcgctcgc tcatggatag gctgccgccg
      601 caggagtaag ctagtttcca ttgcagcaat gcgaggccgc catgacaccc tggcaccagt
      661 actggacgca ttggatggaa ctcgcgcgcg aggcgcccga ggaggtcgcg cgccgcgccg
      721 acctgatcac gcgcgccccc ggctcgccgc tctcgctggc cgattcgcag cgtgtgctgt
      781 gggagaactg gaccgccgcc accgagtcct ggtggtcctg gtggaaggcc gcccacacga
      841 tgcaatggcc aaccatgccc tggccgccgg ccggcgtggt gccgccgccc gaagaagcgc
      901 ccgcagcacc tgcgccggca ccggcagccg ctgcaccggc accaaagggc aagcgcgtga
      961 gcgccgcccc tgcggccccg gccgcgcccg accccgccct caaggcgcgg gtcaggcggc
     1021 cccgtcatcg ctaggagatg acgacacccg gtcgcggact gccgcgacca ccctccgaga
     1081 gggtgccggc ttgcttggga gcggcccggc gcagcgccgg cctggcctca gcccgtcaga
     1141 agctgaaggg cttgctcagg ctgacgtaga agccgcgccg cggaccgaac tgcggggcgc
     1201 tgacgccgat gccggtgccg tcgcgcagct cgtagacgcg gtcgaagaca ttgatcaccg
     1261 agaagcgcgc atccagcctg ccgaaaccag cgccgagatc gaagctgcgc gcgacggccg
     1321 cgttgacctg ggtatagctc ggcagatgct ctgaattgac gaagccacgc ctcaggccgc
     1381 tgccgtagat caggtcgctg ctcagcgccg tgccatcggc gaagcgatag gccgcaccca
     1441 tgctggctgc gacgacctgg tcgtggtcca gatgcaccca atggtcggcg atgtaagcga
     1501 gctcgtcgga gccgaagttg aactgacccg tctcgatgtt cctgcccttg gccgatgaga
     1561 aagccaggtt gccgtaggcc gagaagttct ggtgccggta gctggccgag agctccaggc
     1621 cgtagatcct gccccgctcg tagttgaagt tggagaagat cagtgcattg ccgaactggc
     1681 cctcgtcctg cagatgcctc acgtcgcggt aataggcatc ggcacccagc gtgagctgag
     1741 gcgtgagctg gtgcgagatg ccggcatcga agtaattggt gcgctcggac ttgactgccg
     1801 tgttggcatc cgatggcagc gcattggtcg tgccctggaa tttctgcacc gaggtcaggt
     1861 cgatcttctc gctggcgggc ggcgtgaaat agcgcgcata gccggcgtgc agccgcgtgt
     1921 tctcgccgag ctgccaggtc gcgcccaggc gcgggctgag ctgcgactcc ttcaccaccg
     1981 tatcgacctg gtcgtagcgc gcgccgtagt tcacggtcag cgcgggcgtg ggcttccact
     2041 cgtcctgcag atagacgccg tatagatgcc cgctcaggcg gttgttgtcg acgacggtct
     2101 cgggcgtggt gct
//
LOCUS       DASUCY010000414         3937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_956900, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000414 DASUCY010000000
VERSION     DASUCY010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956900"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="VFV25_08300"
     CDS             <1..996
                     /locus_tag="VFV25_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HEY1393365.1"
                     /translation="AVYTLIGALVWLTNERHHKSMPIQGLEVEEVERGAAVHWIERLM
                     PPVVTGAVVAVIGLNLASVPIKNMAPTPFDTWMQAVTFLCVGAVAVYTRGMVQRLLIL
                     VGLALATIVYAVLANGLGLGQPLDFTKLVNAPWFGWPHFASPVFDPEAMLLIAPVAVI
                     LVAENLGHIKAVAAMTGRDLNPYLGRAFIGDGVATMVSGAAGGSGVTTYAENIGVMAA
                     TKIYSTAVFVVAGLIAVLLGFSPKFGALIQTVPLAVMGGVSIVVFGLITIAGAKIWVD
                     NKVDFTNNANIIVAAITLVLGTGNYSLTLGRFTLGGIGTATFGAILLHALLKRQR"
     gene            1013..3130
                     /locus_tag="VFV25_08305"
     CDS             1013..3130
                     /locus_tag="VFV25_08305"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY1393366.1"
                     /translation="MPGVDGGRRHKQAVPLALLLACLCAGTAVLAGPTAAPAVALSAS
                     QAASGAPDLAQLRALEDRGRVDPGPAIQALQALRLRTAAGSAQRLELLSLLGLLYARQ
                     SDAAHAEAIANELADWADGGPIAAPLAKSAGLGVRAELASNSDADLAHAEQLAGEALQ
                     LLPAEPAPAYGRERLRLLGLLSSVQIQRGRLDASVLTAQEQVRIADALGDLVSRSRTR
                     DNLSYALLRAGQTEGANRLNAEARALADRAGDPAQAANARVTLSILLGQLGDHAGERA
                     AMQDALKLTRQAGWLSQEALLLANLADSFLKTREFQTARDYAEQALALLANAPDRYAQ
                     SGALTNLGLAELGLKRVDAGRQHLEQALSIERRRGARAEEAAILSDMGEALEQAGQPA
                     QAIAAYHRLRELQEEILQGDRQRAVLELQERFDAERRQHELTLLQREGELRDAQLQAQ
                     GLKLRLGLLAVLASALGAAVVVLLVQRTRRVNRELANANALLRIEGGRDALTGLANRR
                     RVQEVMQRRGSVDGTLLLVDLDHFKHINDTAGHAAGDAALLQVAQRLREALREADLLA
                     RWGGEEFLIFLPGMAEDQVEPLVKRLLQSIGGAPVAVAAAANAKREQAQFGLSASIGY
                     ASFPLLPAHVALPWEAALNLVDAALYLAKAKGRNRACGVRLLQAHDAAGVADLRQRLE
                     QANEAGYVALAWTMGPAPEEALA"
     gene            3304..>3937
                     /locus_tag="VFV25_08310"
     CDS             3304..>3937
                     /locus_tag="VFV25_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393367.1"
                     /translation="MIAAAVLLMQGFATPARAFDAEIAAAAHSAAVRPHEAAEALDAL
                     ANRRGDPAARALAAWERGRLALELGERKTARDLAQGLQAQAEPNQRAAGLLLQALSES
                     THGNTDAAARDAHAALKLLDQAEGNAALRSDGLRLLSRAIGSGGDLATALHHLEAAGK
                     LAQAEHDPWREIEALLEQAKLQSLYRHTERAGEALAAARRVEEPHHDPVHL"
BASE COUNT          596 a         1384 c         1402 g          555 t
ORIGIN      
        1 gcggtctata cgctgatcgg cgcgctggtg tggctgacca atgagcgcca ccacaagtcg
       61 atgccgatcc agggcctgga ggtcgaggaa gtcgaacgcg gcgccgccgt gcactggatc
      121 gagcggctga tgccgccggt cgtcaccggc gccgtggtcg cagtgatcgg cctcaacctg
      181 gccagcgtgc cgatcaagaa catggcgccc acgcccttcg acacctggat gcaggccgtc
      241 accttcctgt gcgtcggcgc ggtggcggtc tacacgcgcg gcatggtgca gcggctgctg
      301 atcctggtcg ggctggcgct ggccaccatc gtctacgcgg tcctggccaa cggcctgggc
      361 ctgggccagc cgctggactt cacgaagctg gtcaacgcgc cctggttcgg ctggccgcat
      421 ttcgcaagcc cggtcttcga tcctgaggcg atgctgctga tcgcgccggt cgccgtgatc
      481 ctggtggctg agaacctggg ccacatcaag gcggtggccg cgatgaccgg gcgcgacctc
      541 aacccctacc tggggcgtgc cttcatcggc gacggcgtcg cgaccatggt ttcgggcgcg
      601 gccggcggca gcggcgtcac cacctatgcc gagaacatcg gcgtgatggc ggcgaccaag
      661 atctactcga ccgccgtctt cgtcgtggcg ggcctgatcg ctgttctgct gggcttctcg
      721 cccaagttcg gcgcgctgat ccagaccgtg ccgctggcgg tgatgggcgg cgtgtccatc
      781 gtcgtgttcg gcctgatcac catcgcgggc gccaagatct gggtcgacaa caaggtcgac
      841 ttcaccaaca acgccaacat catcgtcgcg gccatcacgc tggtgctggg caccggcaac
      901 tattcgctga cgctgggccg cttcacgctg ggcggcatag gcaccgcgac cttcggcgcg
      961 atcctgctgc acgcgctgct caagcgccaa cgctgagcgc aagatcggcg cgatgcctgg
     1021 agtcgatggc gggcgcagac acaagcaggc ggtgccgctg gcactgctgc tggcctgcct
     1081 gtgtgcgggc actgccgtac ttgccgggcc gactgccgcc cctgccgtcg ccctgagcgc
     1141 gtcgcaggcc gcatccggcg cacccgacct agcgcaattg cgcgccctcg aagaccgggg
     1201 gcgggtcgat cccgggcccg cgatacaggc cttgcaagcc ctcaggctcc gcaccgcggc
     1261 gggcagcgcg cagcggctgg agctgctgag cctgctgggc ctgctctacg cccgccagag
     1321 cgacgctgcg cacgccgaag cgatcgcaaa cgagctggcc gattgggccg acggcggccc
     1381 gatcgccgcg ccgctcgcga aatcggccgg cctgggcgtg cgggccgagc tggcatcgaa
     1441 cagcgacgcc gatctggccc atgccgagca actggccggc gaagccctgc agctgttgcc
     1501 ggccgagccc gcaccggcct atgggcgcga acgcctgcgg ctgctcggct tgctgagctc
     1561 ggtgcagatc cagcgcggcc ggctggatgc gtccgtgctc acggcccagg aacaggtgcg
     1621 catcgccgat gcactgggcg acctggtgtc gcgctcgcgg acccgcgaca acctgtccta
     1681 cgcgctgctg cgcgccgggc agaccgaggg cgcgaatcgt ctgaacgccg aggcacgggc
     1741 gctggccgac cgagccggcg accccgcgca ggcggccaat gcgcgcgtca cgctatccat
     1801 cttgctgggg cagctgggcg accatgccgg cgaacgcgcg gcgatgcagg acgcgctgaa
     1861 gctcacgcgc caggccggct ggctcagcca ggaggcgctg ctgctggcca acctcgccga
     1921 ctctttcctc aagacccgcg agttccagac ggcgcgcgac tatgccgaac aggccctggc
     1981 attgctcgcc aacgcaccgg accgttatgc gcaatcgggg gcgttgacca acctcggcct
     2041 ggccgaactg ggcctgaagc gcgtggatgc ggggcgacag cacctggaac aggccttgag
     2101 catcgagcgg cgcaggggcg cacgcgcgga ggaagcagcc atcctcagcg acatgggcga
     2161 ggcgctggag caggccggcc agccggcgca ggccattgcc gcctaccacc gcctgcgcga
     2221 gctgcaggaa gaaatcctgc aaggcgaccg ccagcgcgcc gtgctcgaac tgcaggagcg
     2281 cttcgacgcc gagcgccgcc agcatgaact gaccctcttg cagcgcgaag gcgagttgcg
     2341 cgacgcccag ctgcaggccc agggcctcaa gctgcgcctg ggcctgctgg cggtgctggc
     2401 cagtgccctg ggcgcggcgg tggtggtcct gctggtccag cgcacgcgcc gcgtcaatcg
     2461 cgagctggcc aacgccaatg cgctgttgcg catcgaaggc ggacgcgacg cgctgaccgg
     2521 gctggccaat cgccgccgcg ttcaggaggt catgcaaagg cgcggcagcg tcgacggcac
     2581 gctgctgctg gtcgacctcg accacttcaa gcacatcaac gacacggccg gccatgccgc
     2641 gggcgacgcg gcgctgttgc aggtggcgca gcgcctgcgc gaggcactgc gcgaggccga
     2701 cttgctcgcg cgctggggtg gcgaggagtt cctgatcttc ttgcccggca tggccgagga
     2761 tcaggtcgag ccgctcgtga agcgcctgct gcaaagcata ggcggcgccc cggtcgccgt
     2821 cgccgcggcg gcgaacgcga agcgcgagca ggcccagttc gggctgagtg cctccatcgg
     2881 ctatgcgagc tttccgctgt tgccggccca tgtcgccctc ccttgggagg cggcgctgaa
     2941 cctggtggat gcagccctct atctggccaa ggccaagggc cgcaaccgcg cctgcggcgt
     3001 gcggctgctg caggcgcacg acgcggccgg cgtggccgat ctccggcaac gactcgagca
     3061 ggccaacgag gccggctatg tggcgctggc ctggacgatg gggccggcgc ccgaggaggc
     3121 tctagcatga gccaacctga gtcgggccgc cgcgccgggc cgaacagcct accgccagag
     3181 tcggcaggcg ttcgtgccgt cccagccgac gcaggtcggc ttggagccgc ggcactcgcc
     3241 ccaagcaagg cccgctctcc cctcggggga gtggacggcg tactcgccgg ccgaggggcg
     3301 gtcatgatcg ccgcggcggt gctgctgatg cagggcttcg cgactccggc gcgggccttc
     3361 gatgcggaga tcgccgccgc cgcgcattcg gccgccgtcc ggccccatga agccgctgag
     3421 gcgctggacg ccctcgccaa ccgccgcggc gatccagcgg cgcgtgcgtt ggcggcctgg
     3481 gaacgcggcc gtctggcctt ggaactgggc gagcgcaaga cggcgcggga cctggcgcaa
     3541 ggcctgcagg cccaggccga gccgaatcag cgagcggcag gactgctgtt gcaggcactg
     3601 agcgaatcga ctcacggcaa taccgatgcc gcggcccgcg acgcacacgc agccttgaag
     3661 ctgctcgatc aggccgaagg caatgcggcc ctgcgcagcg acgggctgcg gctgctgtca
     3721 cgcgcgatcg gcagcggcgg cgatctggcc accgcactgc atcatctcga agctgccggc
     3781 aagctggccc aggccgagca tgatccctgg cgcgaaatcg aggccttgct ggaacaggca
     3841 aagctgcagt cgctctaccg ccataccgag cgggcgggcg aagcgctggc cgcagcccgc
     3901 cgcgtcgagg agccgcacca cgacccagtg catctgg
//
LOCUS       DASUCY010000415         4904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1085611, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000415 DASUCY010000000
VERSION     DASUCY010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4904
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1085611"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..1331)
                     /locus_tag="VFV25_08315"
     CDS             complement(168..1331)
                     /locus_tag="VFV25_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HEY1393368.1"
                     /translation="MRDALRLNPDVVACWEPHPGEALFRALSALQEHGPVPMLVFTSD
                     AEVEAMERAMAVGVQCWVVQGYGLQRLRSLVQLAQVRFKHERELSDTLEALTHRFEER
                     KLVDRAKGILMRASQVSEDEAFRMLRSASMHGNRRVGQVSQQIIDAARYAEAVNRAGQ
                     LRMLSQRLVKLQALVCAGVEAEASRALLAQSIERAEGNLTELSKLLSKPTFGDLIDGA
                     WASWRELKKALAAVAQPVDLAALDEAAQRLLTDAERFTAALEAAGLASTLHVINLAGR
                     QRMLSQRVAKLALLSSLLDAGAAQHAKQAAQQATAAFEQALQELALMPLSTPEIRAGL
                     AEASQTWQALLAGGQHANQPQGRVALAAASEALLELFDRLTLLYERSMQGLMG"
     gene            1765..3042
                     /locus_tag="VFV25_08320"
     CDS             1765..3042
                     /locus_tag="VFV25_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1393369.1"
                     /translation="MQGFKSFLRSGHAPTLFAAFLYFDFTFAVWVLNGAMGPFISESY
                     HLSPAQKGFMISVPIIAGALMRFPLGVLSQYIGRKNAAMVEMALIAASLAFGYFFVDS
                     YDSVLAMGVLLGIAGASFGVALSLGSGWFPPRYKGLAMGIAGAGNSGTVLAVLFAPPL
                     AAKFGWQTVYGLAACTMALPMLVMWFAAKEPPDREHQTLREHVSCLFEKDGWAFSLIY
                     IITFGGFIGLSSFLPTYFYDQFKVTKIEAGQLTMLATLMGSGLRVVGGYIADRMGGVT
                     TLSGVLLLVAATLVLCGLASASLVVTTLLFMLCFAALGAGNGALFQLVPLRWPLTTAV
                     AGGMIGEIGALGGGFIPNAMGISKQFAGTYLWGFASFALAALLMLAMLRIVQIRWTRT
                     WAEKGGRARTAALPSQASFAVGEASQQTKGVLA"
     gene            complement(3048..4739)
                     /locus_tag="VFV25_08325"
     CDS             complement(3048..4739)
                     /locus_tag="VFV25_08325"
                     /EC_number="2.7.11.-"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-serine/threonine
                     kinase/phosphatase"
                     /protein_id="HEY1393370.1"
                     /translation="MSFDVDIGYASRRGQREANEDFAAALREPGRGLIAAIADGVSAG
                     GQGSLAAQTTVMSLLSDFFAAPAAWETTVALDRLIAAQNAWLATHNRHAAGESAAPAM
                     TTLTALVLQGHACTIAHVGDTRAWRLHGGECQQLTQDHAFEHPDLRSRLSRAVGLDDQ
                     VRVDYLQGDVEIGDVFVLTSDGVHGVLKHRALAAIAAAGTAQQASEALVDAALAAGSR
                     DNATALVIRVLGLDPARLEDELQRGQRLPVPGRLKVGDVLDGYTVTATVADTGVHRLY
                     QARMPGGKLVALKTLHESRAGDPEERAMLVHEAWLGARITERDDRGFVRVHETPDASA
                     LYTLFDWHSGQTLEQWLAQGRRFTVAEIVSAAQEIARALGRLHRHGAIHRDIKPANLH
                     LAEDGQWRVLDLGVALSGREPAAQRVLHAGTPSYMNPEQWEEGGHADAGSDLYALGVT
                     LYRWLTGRLPYGETEPYQTARHRRDPQPPSRLRPDVPIWLDHVVLKAVARDARWRFET
                     AEELALALGRGASRSLSAPPGTPLLRDATGLWQIAFAVSALLNALLIAWLLFLPR"
BASE COUNT          715 a         1728 c         1663 g          798 t
ORIGIN      
        1 actgccaaca acgcatcctg cgcccatgcc atgacagcgg caaggcggga tgtagcgtga
       61 ggctagcccg attgccggac tcagtccaat tgctcccgcc tatgccgcac gatagcctgg
      121 ggttgttacc gaagcccggc cggtcaacac caaggagaga aagagagcta acccatcagg
      181 ccctgcatgc tgcgctcgta caagagcgtc agccggtcga acaattccag cagggcctcg
      241 ctggccgcgg cgagcgcgac acggccctgg ggttggttcg cgtgctgtcc gcccgccagc
      301 agggcctgcc aggtctggct cgcctctgcg aggcctgcgc gaatctcggg agtggacagc
      361 ggcatcaggg ccagctcctg cagcgcctgc tcgaaggcag ccgtcgcctg ctgcgccgcc
      421 tgtttcgcgt gctgcgctgc gccggcgtcg agcagcgagc tcagcagcgc cagcttggcg
      481 acccgctgcg acagcatgcg ctgcctgccg gcgagattga tgacatgcag cgtgctggcc
      541 agcccggccg cttcgagtgc ggccgtgaag cgctccgcat cggtgagcag ccgctgcgcc
      601 gcttcgtcca gggcagccag gtcgaccggc tgtgccactg cggccagtgc cttcttcagt
      661 tcgcgccaag aggcccaggc cccatcgatc aggtcgccga aggtgggctt ggacaagagc
      721 ttgctcagct ccgtcagatt accctcggcg cgttcgatgg actgcgcgag caaggcgcgg
      781 cttgcctccg cctcgacccc ggcgcagacc agggcctgca gcttgaccag gcgctgcgac
      841 agcatgcgca gctgcccagc ccggttcacc gcctcggcat agcgcgccgc atcgatgatc
      901 tgctgcgaga cctgcccgac gcggcggttg ccgtgcatcg acgcgctgcg cagcatcctg
      961 aaggcctcat cctcggacac ctggctagcg cgcatcagga tgcccttggc gcggtccacc
     1021 agcttgcgct cctcgaagcg gtgggtgagc gcctccagcg tgtcgctcag ctcgcgctcg
     1081 tgcttgaagc gaacctgcgc cagctgcacc agggagcgca gcctctgcag gccgtagccc
     1141 tgcacgaccc agcactgcac gccgacggcc atggcccgct ccatcgcctc cacctcggca
     1201 tcgctggtga ataccagcat cggcaccggc ccgtgctcct gcagcgcgct caaggcgcga
     1261 aacagcgcct cgccggggtg aggctcccag cacgcgacca cgtcggggtt cagcctgagg
     1321 gcgtcgcgca ccagtttgtc gcacgcagtc gcaccaagaa cgtgaaaacc gaccgacgcg
     1381 aagtcatccg ccagcgccgg cgcgccggga tcgtgatcga taacaagcaa tacggaagtc
     1441 aagatggcca gcgcaggagc gaacgagagc gcacgacgcg cccgggacag tatgaagtgc
     1501 aaggagcggg cctgcacccc taacccagtg cacgccgtgg atccggctcc gccgggccac
     1561 tggcgtggcc cccttgaggg ggcgcgcgcc agcgcgtagg gggtgggccc aacctggcac
     1621 gcctgttgca ttgcaccatg cacacgggtg tgccgctgca cccggaccga gcgccagcac
     1681 cgccggtctt gcgcatgacg ccgtgcgcag ccatgtgcct ccccgacccg cctctgtgcg
     1741 gttcccttcg tctcggagag caacatgcaa ggcttcaagt cctttcttcg cagcggacat
     1801 gccccgactc tgttcgcggc cttcctctac ttcgacttca ctttcgctgt ctgggtcctc
     1861 aacggcgcga tgggcccttt catcagcgag agctatcacc tgagcccagc gcagaagggc
     1921 ttcatgatct cggtgcccat catcgcgggc gcgctgatgc gcttcccgct gggcgtgctg
     1981 tcgcaataca tcggccgcaa aaacgcggcc atggtggaga tggcgctgat cgcggcgtcg
     2041 ctggccttcg gctacttctt cgtcgacagc tacgacagcg tgctcgcgat gggcgtgctg
     2101 ctgggcatcg ccggcgcgag cttcggcgtg gccctgagcc tgggcagcgg ctggttcccg
     2161 ccgcgctaca agggcctggc catgggcatc gccggcgccg gcaattcagg cacggtgctg
     2221 gcggtgctgt tcgcgccgcc gctggccgcc aagttcggct ggcagacggt ctatggcctg
     2281 gcggcctgca ccatggcgct gccgatgctg gtgatgtggt tcgccgccaa ggagccgccc
     2341 gatcgtgagc accagacgct gcgcgagcat gtgtcctgcc tgttcgagaa ggacggttgg
     2401 gccttcagcc tgatttacat catcaccttc ggcggcttca tcggcctgtc cagcttcctg
     2461 cccacctatt tctacgacca gttcaaggtc accaagatcg aagccggcca gctgaccatg
     2521 ctcgccacgc tgatgggttc gggcttgcgc gtagtcggcg gctatatcgc cgaccgcatg
     2581 ggcggcgtga ccacgctcag cggcgtgctg ctgctggtgg cggccacgct ggtgctgtgc
     2641 ggcctggcca gcgcttcgct ggtggtcacc actttgctct tcatgctgtg ctttgccgcc
     2701 ttgggcgcgg gcaacggcgc cttgttccag ctggtgccgc tgcgctggcc gctcacgacg
     2761 gcggtggcgg gcggcatgat cggcgaaatc ggcgccctgg gcggtggctt catcccgaac
     2821 gcgatgggca tttccaagca gttcgccggc acctatctct ggggcttcgc gtccttcgcg
     2881 ctggccgcgc tgctgatgct ggcgatgctg cgcatcgtgc agatccgctg gacccggacc
     2941 tgggcggaaa agggcggccg ggcccggacc gctgcgctgc cgtcgcaggc cagcttcgcc
     3001 gtgggcgagg ccagccagca gaccaaggga gtcctggcgt gacaacgcta gcgcggcagg
     3061 aagagcagcc acgcgatcag cagcgcgttg agcagcgccg agaccgcgaa ggcgatctgc
     3121 cacaggccgg tggcgtcgcg cagcagcggc gtgccgggcg gcgcgctcag gctgcgcgag
     3181 gcgccgcgcc ccagggccag cgccagctct tcggccgtct cgaagcgcca gcgcgcatcg
     3241 cgcgccacgg ccttcagcac cacatggtcc agccagatgg gcacatcggg ccgcagccgc
     3301 gagggcggct gcggatcgcg ccgatggcgg gccgtctggt agggctcggt ctcgccgtag
     3361 ggcaggcggc cggtcagcca gcggtacagc gtgacgccca gggcgtagag gtcgctgccc
     3421 gcatcggcat ggccgccctc ctcccattgc tccgggttca tatagctggg cgtgccggca
     3481 tgcagcacgc gctgcgccgc gggctcgcgc ccggacaagg ccacgccgag atcgaggacg
     3541 cgccactggc cgtcctccgc cagatgcagg ttggccggct tgatatcgcg atggattgca
     3601 ccatgtcggt gcagcctgcc caaagcgcgg gcaatctcct gtgcagcgga cacgatctcg
     3661 gccaccgtga agcgccggcc ttgagccagc cattgctcca gcgtctgccc gctgtgccag
     3721 tcgaacaagg tgtacagggc gctggcatcg ggcgtctcgt gcacgcgcac gaagccgcgg
     3781 tcgtcgcgct cggtgatgcg cgcccccagc caggcttcat gcaccagcat cgcgcgctct
     3841 tcagggtcgc cggcgcgcga ctcgtgcagg gtcttcagcg ccaccagttt gccgccgggc
     3901 atgcgcgcct ggtagaggcg atgcacgccg gtgtcggcca cggtcgcggt caccgtgtag
     3961 ccgtccagca cgtcgcccac cttcagtcgg cccggcacgg gcagccgctg tccgcgctgc
     4021 agttcgtcct cgagccgcgc aggatccagg cccagcacgc ggatcaccag cgccgtcgcg
     4081 ttgtcgcggc tgccggcggc cagcgcggcg tccaccagcg cctcgctcgc ctgctgggcc
     4141 gtgcctgcgg ccgctatcgc agccagcgcg cggtgcttga gcacgccatg cacgccatcg
     4201 ctggtcaaca cgaagacatc gccgatttca acgtcgcctt gcaggtagtc cacgcgcacc
     4261 tggtcatcca gccccaccgc gcggctgagc cggcttctca ggtcgggatg ctcgaaggcg
     4321 tggtcctggg tgagctgctg gcattcgccg ccgtgcagcc gccaggcgcg cgtgtcgccg
     4381 acgtgggcga tcgtgcaggc gtgcccctgc agcaccagcg cggtgagcgt ggtcatggcc
     4441 ggcgcagccg attcgcccgc cgcatggcgg ttgtgcgtgg ccagccaggc gttctgcgcc
     4501 gcgatcagcc ggtccagcgc gaccgtggtt tcccaggcgg ccggcgcggc gaagaagtcg
     4561 ctcagcaggc tcatgaccgt ggtctgagcc gccaggctgc cctgcccgcc ggccgacacg
     4621 ccgtccgcga tcgccgcgat cagcccgcgc cccggctcgc gcagcgcggc ggcgaagtcc
     4681 tcgttggctt cgcgctggcc gcgccggctg gcgtatccga tatcgacatc gaagctcatc
     4741 ggctcccctg ttgtgcgcgg cagccgcgcg tcgggtccat ggcgcgcgat tgtcttacgt
     4801 gtttcccggc acccggcctg ccaaagatgg ccgctctcgt ggagtgatgt gatgaagaag
     4861 ctgaagctgg tgatggtggg caacggcatg gccggcgtgc gcac
//
LOCUS       DASUCY010000416         2770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1326424, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000416 DASUCY010000000
VERSION     DASUCY010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1326424"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VFV25_08330"
     CDS             complement(<1..217)
                     /locus_tag="VFV25_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY1393371.1"
                     /translation="MRILLAEDEKPLADRLVKALGQNDFVVDWIDDGRLVLNQLQATR
                     YDALILDLGLPGRGGQRGARAVARGGPA"
     gene            301..1473
                     /locus_tag="VFV25_08335"
     CDS             301..1473
                     /locus_tag="VFV25_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY1393372.1"
                     /translation="MLIDFFYTLRSAKLPVSIKEYLMLLEAVKAGVIGPSIDEFYYLA
                     RASLVKDEANYDKFDRAFGAYFKGVESIADFTQDIPLEWLQKKFELELSPEEKAAIEK
                     MGWDELMETLKKRFEEQKERHEGGSKWIGTGGTSPFGAYGVNPQGVRIGQDKGRNRSA
                     VKVWDQRAYKDYDDTLELGTRNIKVALRRLRRFAREGAAEELDLEDTIHSTAANAGML
                     DIKMVPERHNKVKVLLLMDVGGTMDEHIHRVEELFSAAKSEFKHLEFYYFHNCVYDFM
                     WKNNRRRFSEKFPTWDVIRKYNKDYKLVFVGDATMSPYEILQPGGSVEYNNEEPGAEW
                     LQRLTHAFPKYAWINPEPQGVWGYRQSVSIIQQLMNQRMFPLTLAGLEGAMRLLSK"
     gene            complement(1578..1964)
                     /locus_tag="VFV25_08340"
     CDS             complement(1578..1964)
                     /locus_tag="VFV25_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393373.1"
                     /translation="MPIRIASLSQRIAKNGAMLHQAMMDRVHSLGADHEPSEAEQTLA
                     EERRLLKEERAKRQAQAQQPRVKAVDKLVRTHHPQAKEKPKAKKEPKEVAKAGKAHRP
                     SRAEKHAAKASALQAASRAPKKSAKA"
     gene            2472..>2770
                     /locus_tag="VFV25_08345"
     CDS             2472..>2770
                     /locus_tag="VFV25_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HEY1393374.1"
                     /translation="MSWTLVVKAPDRVRELLLRYLELARFQHGDGDITRAELSRLAAA
                     APAQARSLLETEGYFEPKIGISLATEDPPTVVLEVEPGPRATIAALSLKVEGDLQT"
BASE COUNT          480 a          929 c          886 g          475 t
ORIGIN      
        1 gcgcaggtcc gcctcgcgca actgcgcgag cacctcgttg cccaccgcgg cccggcaggc
       61 cgaggtcgag gatcaaggcg tcgtagcgag tggcctgcag ctgattgagc accaggcggc
      121 cgtcatcgat ccagtcgacg acgaaatcgt tctgacccag agccttgacc agccggtcgg
      181 ccagcggctt ctcgtcttcg gcaagcagaa tgcgcatggg cgccttccta attctgtgga
      241 catggctttc gccagtggtc gcacaatccc cataacgccg ccttcgctac acccgccgcc
      301 atgctgatcg acttcttcta cacgctgcgc tcggccaagc tgccggtctc gatcaaggaa
      361 tacctgatgc tgctcgaggc cgtgaaggcc ggcgtgatcg ggccatcgat cgacgagttc
      421 tactacctcg cgcgcgcatc gctggtgaag gacgaggcca actacgacaa gttcgaccgc
      481 gccttcggcg cctatttcaa gggcgtggag agcatcgccg acttcacgca ggacatcccg
      541 ctggagtggc tgcagaagaa gttcgagctc gagctcagcc ccgaggagaa ggcggcgatc
      601 gagaagatgg gctgggacga gctgatggag acgctcaaga aacgcttcga ggagcagaag
      661 gagcgccacg agggcggcag caaatggata ggcaccggcg gcaccagccc cttcggcgcc
      721 tacggggtca acccgcaggg cgtacgcatc ggccaggaca agggccgcaa tcgcagcgcc
      781 gtcaaggtct gggaccagcg cgcctacaag gactacgacg acacgctgga actcggcacg
      841 cgcaacatca aggtagcgtt gcggcgcctg cgccgcttcg cgcgcgaagg cgcggccgag
      901 gagctagacc tggaagacac catccattcc acagcggcca acgccggcat gctggacatc
      961 aagatggtgc ccgagcgcca caacaaggtg aaggtgctgc tgctgatgga cgtgggcggc
     1021 acgatggacg agcacatcca ccgcgtcgag gagctgttca gcgctgcgaa gagcgagttc
     1081 aagcacctcg agttctatta cttccacaac tgcgtctacg acttcatgtg gaagaacaac
     1141 cggcggcgct tctccgagaa gttcccgacc tgggatgtga tccgcaagta caacaaggac
     1201 tacaagctgg tcttcgtcgg cgacgcgacg atgagcccct atgaaatcct ccagcccggc
     1261 ggcagcgtcg aatacaacaa cgaggagcct ggcgccgaat ggctgcagcg cctcacccac
     1321 gccttcccga aatacgcctg gatcaacccc gagccgcagg gcgtgtgggg ctaccgccag
     1381 agcgtgtcca tcatccagca gctgatgaac cagcgcatgt tcccgctgac gctggcgggg
     1441 ctggaggggg caatgaggtt gttgagtaag tagggcttag ggctgcgtcg acgttcggtg
     1501 ccggccgatg cccgttaacg agccggctta ggggccggcg cggagcgcgt ccgcgctccg
     1561 cgcgctgtct gcgcgcctca ggctttcgcc gacttcttcg gcgctctact cgcggcctgc
     1621 agggccgacg ccttcgcggc gtgcttctcg gcacgcgagg gcctgtgggc cttgccagcc
     1681 ttggccacct ccttgggttc cttttttgcc ttgggctttt ccttggcctg cgggtggtgc
     1741 gtcctcacca gcttgtcgac ggccttcacc ctcggctgct gcgcttgcgc ttggcgcttc
     1801 gcccgctctt ccttgagcag gcggcgctct tcggcgagcg tctgttcggc ttcggacggc
     1861 tcatggtccg cccccagcga gtggacgcgg tccatcatcg cctggtgaag catcgcgccg
     1921 ttcttcgcga tgcgctggct cagcgatgcg atgcgaatgg gcatggtgca gtccaaatgg
     1981 tgggtgaagg accaatgtag gccagcccgc cgcgttctgc caatcgattt gtgtcgcttc
     2041 gcccttgcag ccgggactct gctgcagcaa acagccgccc cgtgcgttcg gcggcgcatt
     2101 ggcaggtacc gaggtcggtg tacgcgaacc ccttcgtgca gccgagcaca gagacgattc
     2161 caaatacgcc cagtaaactg ccctctcccc gacccgcaag ccgatgtacg cagccggcgc
     2221 gcctcgatgc agcctgattg ccctctggtc catctgaccc tcgccgcgcc ttcatcacgt
     2281 cgcactgcgc gaacgcgcct gcgaggccga tgagtctgcg ggcgctgctg cttcgcttgg
     2341 ctttgtcagt catcgcgctg gccggctcgc ccttgcgcac cacggcgcag gaagtcccgc
     2401 cgcccgcagc acaagcccca gcctccgccc ccactgcgac tacgccccct gccgacgacg
     2461 ctgcgcaagg cgtgagctgg accctcgtcg tgaaagcccc cgatcgcgtg cgcgagctgc
     2521 tgctgcgcta tctggagctg gcgcgcttcc agcatggcga cggcgacatc acgcgcgccg
     2581 aactttcgcg gctggccgcg gcggcgccgg cgcaagcgcg cagcctgctg gagaccgagg
     2641 gctatttcga gccgaagatc ggcatcagcc tggccaccga ggatccgccc accgtggtgc
     2701 tggaagtcga gcccgggccg cgcgcgacga tcgccgcgct ctcgctgaag gtcgagggtg
     2761 atctgcagac
//
LOCUS       DASUCY010000417         2031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1294697, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000417 DASUCY010000000
VERSION     DASUCY010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294697"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1286)
                     /locus_tag="VFV25_08350"
     CDS             complement(<1..1286)
                     /locus_tag="VFV25_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY1393375.1"
                     /translation="MQYFVTGATGFIGKRLVKKLLARRGAVVHFLLRPESEAKLSELL
                     AYWGVSKARAVPVHGDLTSKKLGVAADAVKALKGQIDMVYHLAAVYDLEADETAQVQV
                     NVEGTRNVVEFAKAIDAGHLHHVSSIAAAGLYEGVFREDMFEEAENFDHPYFMTKHES
                     EKIVRQECKIPWTIYRPAMVVGDSTTGEMDKIDGPYYFFKLIQRMRQLLPPWMPSIGL
                     EGGRVNIVPVDFVVDALDCISHGKGELNRRCFHLVDPVGYRVGDVLDIFSKAAHAPKM
                     NLFVNAALFGFIPKSVKKGLMALAPVRRVRNAVMKDLGLPEDMLSFVNYPTRFDCRDT
                     LAALKGTGIACPNLRDYAWRLWDYWERHLDPDLFIDRSLNGKVRGRVVLISGGSSGIG
                     KAAAQKVAAAGAKVLIVARGEEELAATRKEIVDAGG"
     gene            1508..2023
                     /locus_tag="VFV25_08355"
     CDS             1508..2023
                     /locus_tag="VFV25_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEY1393376.1"
                     /translation="MVKKLQKMAEKKSGSAAGLFDSQFAGTVKDSAQQIWLAGLGAFA
                     KAQEEGGKVFDALVKEGVAIQRKTQAVAGEKFGDVAGKMTKLSGEVQAKAGQHWDKLE
                     TIFEERVAKALNKLGVPSAKDVEALIKRIDELSAAVGKASAAKPAAARKKTAARPAAK
                     RSSRPARKSAA"
BASE COUNT          386 a          674 c          641 g          330 t
ORIGIN      
        1 tcgccgccgg cgtcgacgat ctccttcctg gtcgccgcaa gttcctcctc accgcgcgcc
       61 acgatcagca ccttggcgcc ggccgcagct actttctgcg cggcggcctt gcctatgccg
      121 gacgaaccgc cgctgatcag caccacgcgc ccgcgcacct tgccgttgag cgagcggtcg
      181 atgaacaggt ccggatcgag gtggcgctcc cagtagtccc acagccgcca ggcgtagtcg
      241 cgcagattgg ggcaggctat gcccgtgccc ttgagggcgg ccagcgtatc gcggcagtcg
      301 aagcgcgtcg gatagttgac gaagctcaac atgtcctcgg gcaagccgag atccttcatc
      361 accgcattgc gcacgcggcg caccggcgcc agcgccatca agcccttctt cacactcttg
      421 gggatgaagc cgaacaaggc tgcgttgacg aagagattca tcttgggcgc atgcgcggcc
      481 ttggagaaga tgtccagcac atcgccgacg cggtagccca ccgggtcgac cagatggaaa
      541 cagcgccgat tgagctcgcc cttgccgtgg ctgatgcaat ccagcgcgtc gacgacgaag
      601 tccaccggca cgatgttgac gcgccctcct tccaggccga tcgagggcat ccagggcggc
      661 agcagctggc gcatgcgctg gatcagcttg aagaagtagt aaggcccgtc gatcttgtcc
      721 atctcgccgg tggtggagtc gcccaccacc atcgccggcc gatagatggt ccagggaatc
      781 ttgcattcct ggcgcacgat cttctcggac tcgtgcttgg tcatgaagta ggggtggtcg
      841 aagttctcgg cctcctcgaa catgtcctcg cggaacacgc cctcgtagag gccggctgcg
      901 gcgatcgagc tcacgtgatg caagtgaccc gcatcgatgg ccttggcgaa ctcgacgacg
      961 ttgcgggtgc cttcgacgtt gacctgcacc tgcgcagtct cgtcggcctc caggtcgtag
     1021 accgccgcca ggtgatagac catgtcgatc tggcccttga gggccttcac ggcatcggcc
     1081 gcgacgccca gcttcttgct cgtcagatcg ccatggacag gaacagcacg cgccttgctg
     1141 acgccccagt aggccaacag ctccgacagc ttggcctcgc tctcggggcg cagcaggaaa
     1201 tgcacgacgg cgccgcggcg cgccagcagc ttcttgacca gacgcttgcc gatgaagccg
     1261 gtggccccgg tgacgaaata ctgcatgctg ttctcctccc tgagtccggg caacgggata
     1321 aagaagtcga ttcttcacga aggcccgtca gcccacgctg cgcgctttcc ccaagcggca
     1381 tagagcgcgc gccctagaga tttaggctgc cctgcccagc gccgcttctt atagttgcat
     1441 cagacgacat cagcggcgcc gcagcgttcg caccactccc gcaaactcgc tctcccggaa
     1501 ggcaatcatg gtcaagaagc tccagaagat ggcagaaaag aaatccggct cggccgccgg
     1561 cctgttcgac agccagttcg ccggcacggt aaaggactcg gcccagcaga tctggctggc
     1621 cggcctgggc gcctttgcca aggcccagga agaaggcggc aaggtcttcg atgcgctggt
     1681 gaaggaaggc gtggcgatcc agcgcaagac ccaggccgtg gccggggaga agttcggcga
     1741 cgtggccggc aagatgacca agctctctgg cgaggtccag gccaaggccg gccagcattg
     1801 ggacaagctc gagaccatct tcgaggagcg cgtcgccaag gccctgaaca agctgggcgt
     1861 gccttcagcc aaggacgtgg aggcgctcat caagcgcatc gacgagctca gcgcggccgt
     1921 gggcaaggcc tctgccgcga agccggccgc ggccagaaag aagaccgccg ccagacctgc
     1981 agccaagcgc agcagccggc cggcgcgcaa gtcggctgcc tgaaagccag a
//
LOCUS       DASUCY010000418         2274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_860845, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000418 DASUCY010000000
VERSION     DASUCY010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_860845"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1069)
                     /gene="ald"
                     /locus_tag="VFV25_08360"
     CDS             complement(<1..1069)
                     /gene="ald"
                     /locus_tag="VFV25_08360"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012404270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEY1393377.1"
                     /translation="MRIGVPKEIKVHEYRVGLTPSSVRELVAHGHQVLVQRGAGIGIG
                     ASDEDYLQAGAGLAEDAAGVFGQADMIVKVKEPQPEERRLLREGQVLFTYLHLAPDPE
                     QCKDLIASGAVCIAYETVTQPGGGLPLLAPMSEVAGRMAVQAGAHHLEKAQGGLGVLL
                     GGVPGVAPARIVILGAGVVGSNAAQIAVGTGAQVTVIDRSLDALRRMDQLLGARVCTL
                     FSNRDNIEQQVLGADLVIGAVLIPGAAAPRLVTRAMLSQMKPGAVIVDVAIDQGGCFE
                     TSRPTTHAEPTYVVDGVVHYCVANMPGAVPRTSTHALNNATLPFALQLADLGWRAALK
                     RNPHLMAGLNVAGGKVTNKEVA"
     gene            1452..>2274
                     /locus_tag="VFV25_08365"
     CDS             1452..>2274
                     /locus_tag="VFV25_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen-debranching protein"
                     /protein_id="HEY1393378.1"
                     /translation="MPLFRNLLAAALLCAASAQLPATAAINAYQLGAKYDATQTSITF
                     NIYSSRASRVELWLYTSQTGVQEAARVPLSLGANNIWTTTLSTSTLASYGITGTVYYG
                     YRAWGPNWTYDPSWTKGSSVGFVSDVDSAGNRFNPNKLLIDPYALELSHDPLTTSMND
                     LTVYASGASYRNIDNGSVAPKSIVLTGDSQSIGTKPTRAFKDDVVYEVHVRGLTMNDS
                     SVASAYQGTYQGAAQKAAYLAGLGVTAVEFLPVQETQNDNNDVNPTSSANKNYWGY"
BASE COUNT          389 a          778 c          738 g          369 t
ORIGIN      
        1 gggcgacttc cttgttggtg accttgccgc cggccacgtt gagcccggcc atcaagtgtg
       61 ggttgcgctt cagcgccgcg cgccagccta ggtcggccag ctgcagcgcg aagggtaagg
      121 tggcgttgtt cagcgcatgc gttgaggtgc gcggcacggc accgggcatg ttggcgacgc
      181 agtagtgcac gacgccgtcc accacatagg tcggctcggc gtgggtggtg ggacgcgagg
      241 tttcgaagca gccgccctgg tctatggcca cgtcgacgat cacggcgccc ggcttcatct
      301 gcgacagcat cgcgcgcgtc accagccggg gcgccgcggc gccggggatc agcaccgcgc
      361 cgatcaccag gtcggcgccc agcacctgct gctcgatgtt gtcgcggttg gagaacagcg
      421 tgcacacgcg cgcgccgagc agctggtcca tgcggcgcag cgcgtcgagc gagcggtcga
      481 tcacggtcac ctgcgcgccc gtgcccactg cgatctgcgc cgcgttggag ccgaccacgc
      541 cggcgccgag gatcacgatc cgggccggcg cgacgccggg cacgccgccc agcaatacgc
      601 ccagcccgcc ctgcgccttc tccaggtgat gcgcgccggc ctgcaccgcc atgcggccgg
      661 ccacctcgga catcggcgcc agcagcggca ggccgccgcc gggctgggtg acggtctcgt
      721 aggcgatgca gacggcgccg ctggcgatca ggtccttgca ctgctcagga tcgggcgcga
      781 ggtgcagata ggtgaagagg acctggccct cgcgcagcag gcggcgctcc tcgggctggg
      841 gctccttgac cttgacgatc atgtcggcct ggccgaagac gcccgcggcg tcctcggcca
      901 ggccggcgcc ggcctgcagg tagtcctcgt cgctcgcgcc gatgccgatg ccggccccgc
      961 gctgcaccag cacctggtgc ccatgggcga ccagttcgcg caccgacgaa ggcgtgaggc
     1021 cgacacggta ctcgtggacc ttgatttctt tgggtacgcc gatgcgcatg gccgtctccg
     1081 ttggcagggc gcgccggtag gcgctccgtt ttgctttggg cgtgcgcggg tcacgctgca
     1141 cacattgcga accattatgc gtaacaaggc tcgccttttg gcttgtcaag tcggtgcggc
     1201 tcgcgctgag cgtgcggtgg gtgcgaaccg aagggcggat cggctatgta atctgaatac
     1261 agatactttc cgggagtgcc cgattgaagg atgctgttcg tgacgtattg cgcgatttgg
     1321 tgcagcgcat cagggaaaaa ctagggtttg tgattgtgga ttgaatatct agttttagta
     1381 caaattcgcg ccggggaagt agatgagagg ccttcaaggc ccatagcaaa acagccacta
     1441 cggagacacc catgcccctg ttccgcaacc tgctggcggc ggccctcctc tgcgccgctt
     1501 ccgcccagct ccctgccaca gccgcgatca acgcctacca gctgggcgcc aagtacgacg
     1561 ccacgcagac ctccatcacc ttcaacatct actcgtcgcg ggccagccgc gtcgagctct
     1621 ggctctacac ctcgcagacc ggcgtgcagg aggcggcgcg cgttccgctc agcctgggcg
     1681 ccaacaacat ctggacgacg acgctgtcca cctcgacgct ggccagctac ggcatcaccg
     1741 gcacggtcta ctacggctac cgcgcctggg gccccaactg gacctacgac ccgagctgga
     1801 ccaagggctc gagcgtcggc ttcgtcagcg acgtcgacag cgcgggcaat cgcttcaatc
     1861 ccaacaagct gctgatcgac ccctatgcgc tggagctcag ccacgacccg ctgacgacca
     1921 gcatgaacga cctgacggtc tacgcctcgg gcgcttccta tcgcaacatc gacaacggca
     1981 gcgtcgcgcc caagagcatc gtgctcaccg gcgattcgca gtccatcggc accaagccca
     2041 cgcgcgcctt caaggacgac gtggtctatg aggtccacgt tcgcggactg acgatgaacg
     2101 acagcagcgt cgcctcggcc taccagggca cctaccaggg cgcggcccag aaggcggcct
     2161 atctggccgg cctcggagtc accgcggtgg agttcctgcc ggtgcaggaa acgcagaacg
     2221 acaacaacga cgtcaacccc accagttccg ccaacaagaa ctactggggc taca
//
LOCUS       DASUCY010000419         2667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_539814, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000419 DASUCY010000000
VERSION     DASUCY010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539814"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /locus_tag="VFV25_08370"
     CDS             complement(<1..979)
                     /locus_tag="VFV25_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1393379.1"
                     /translation="MSLLGRPPCDEGAILGGAEASPCTRAARRWTLVAAILGSSMAFI
                     DGTVVNVALPALQRDLQATASQAQWVIESYALLLGCLLLVGGALGDRYGRRRVFMLGV
                     GLFTAASFACALAGSIGQLIAARAIQGLGAALLVPGSLALISATFPQQERGKAIGVWS
                     GFSGITAAAGPVLGGFLIDHYSWTWAFLVNLLPGIALLILCAAKVPESRGPETAGRLD
                     VAGAALATLGLAGVAFAFIEAPARGWAAPAVMGAAFAGVAALAAFVMVESRAHEPMLP
                     LGLFRIRSFAGANLLTLLLYAALGGGLYFFPLNLIQVQGYGATAAGAAML"
     gene            1091..1573
                     /locus_tag="VFV25_08375"
     CDS             1091..1573
                     /locus_tag="VFV25_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HEY1393380.1"
                     /translation="MELITVRIDKPADTNFILGQSHFIKTVEDLHEAIVQTAPQMKFG
                     IAFCESSGPALVRWTGNDEELIALAKQNALELSCGHSFIIFISQGFPINILNTIKAVP
                     EVCGIYCATANPVEIIVAETQGGRGILGVIDGMKSAGIEAEPDIQARKQLLRKFGYKL
                     "
     gene            1697..>2667
                     /locus_tag="VFV25_08380"
     CDS             1697..>2667
                     /locus_tag="VFV25_08380"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010092338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEY1393381.1"
                     /translation="MDVREGFIQAVGRTPLIKLRKASELTGCTILGKAEFMNPGGSVK
                     DRAALFIVLDAEARGELRPGGVIVEGTAGNTGIGLALVGNARGYRTVIVIPQTQSQEK
                     KDALRLCGADLREVPAAPYSDPNNYVHVAERLAAELARSEPNGVLYANQWDNLSNRDG
                     HVRSTGPEIWQQTDGRIDGFTCAIGTGGTLAGVGLYLKQHKPGVVIAAADPMGAAIYH
                     YVKHGELRAEGSSITEGIGVGRITRNLEGAPIDDAYQITDEEALPLLFDLLREEGLCL
                     GGSSGINVAGAIRMAHALGPGHTIVTILADAGSRYQSKLYNPAFLRE"
BASE COUNT          494 a          921 c          894 g          358 t
ORIGIN      
        1 gcagcatcgc ggcgccggca gcggttgctc catagccctg gacctgtatg aggttcagcg
       61 gaaagaagta cagcccgccg cccagcgccg catacagcag cagcgtcagc aaattcgctc
      121 ccgcgaagct gcggatgcga aagaggccga gcggcagcat gggctcatgc gcgcgggatt
      181 ccaccatcac gaaggccgcc agcgccgcaa cgccagcgaa ggccgcgccc atcacggccg
      241 gggcggccca ccctcgtgcc ggcgcctcga tgaacgcgaa ggcgaccccg gccagcccca
      301 gcgtcgccag cgccgcgccc gccacgtcga ggcggcccgc cgtttcgggg ccgcggcttt
      361 ccggcacctt ggcggcgcac aggatcagca gcgcgatccc gggcagcagg ttgaccagga
      421 aggcccaggt ccatgaatag tgatcgatga gaaagccgcc cagcaccggt ccggcggcgg
      481 ccgtgatgcc gctgaatcct gaccacacgc ctatggcctt gccacgctcc tgctgcggga
      541 aggtggcgct gatcaatgcc aggctgccgg gcacgagaag tgcggcgccc aggccctgga
      601 tggcgcgggc ggcgatcagc tggccgatgc tgcccgccag cgcgcaggcg aaggacgcag
      661 cggtgaagag cccgacgccc agcatgaaga cccggcgccg gccgtagcga tcgcccagag
      721 ccccacccac cagcagcagg cagcccagca gcagcgcata ggactcaatg acccattgcg
      781 cctgcgaggc cgtggcctgg aggtcgcgct gcagcgccgg cagcgcgaca ttgaccaccg
      841 tgccgtcgat gaaggccatg ctcgagccga ggatggccgc caccagcgtc cagcgccgcg
      901 cggcccgcgt gcagggcgat gcctcagcgc caccgaggat ggcgccttcg tcgcaaggag
      961 gtctgccgag gaggctcatg aggaatcgct aggcagcgcg aggatagccc cctcggccga
     1021 aatacgccac gctgcgattt cagccaccag cgcctattct tttggttccg gtttctgctt
     1081 ggagcaaacc atggaactga tcaccgtccg catcgacaag ccggccgaca ccaatttcat
     1141 cctcggccag tcgcacttca tcaagacggt cgaagacttg cacgaagcga tcgtgcagac
     1201 cgcgccgcag atgaagttcg gcatcgcgtt ctgcgaatcg agcggcccgg cactggtgcg
     1261 ctggaccggc aacgacgagg agctcattgc gctggccaag cagaacgccc tggagctgag
     1321 ctgcgggcac agcttcatca tcttcatcag ccagggcttc ccgatcaaca tcctgaacac
     1381 gatcaaggcg gtgccagagg tctgcggcat ctactgcgcc actgccaatc cggtcgagat
     1441 catcgtggcc gagacgcagg gcgggcgcgg catcctcggc gtgatcgacg gcatgaagag
     1501 cgcgggcatc gaagccgagc ccgacatcca ggcgcgcaaa cagctgctgc gcaagttcgg
     1561 ctacaagctt tgagccgacg gcgacgcggg tcggcgtcgc ctatgccggc gggaatacag
     1621 ccgtcgggct cagggtctat cgcggaccgt tgacaaggcg cgaggcgggg cgtagcactc
     1681 ggttgcgggg gtatgcatgg acgttcgcga aggcttcatc caggcggtgg gccgcacgcc
     1741 gctgatcaag ctgcgcaagg cctcggagct gaccggctgc accatcctcg gcaaggccga
     1801 gttcatgaat cccggcggct cggtcaagga ccgcgcagcg ctgttcatcg tgctcgatgc
     1861 cgaagcgcgc ggcgaattgc gcccgggcgg cgtgatcgtc gagggcacgg ccggcaacac
     1921 cggcatcggc ctggcgctgg tgggcaatgc ccgcggctac cgcacggtga tcgtgatccc
     1981 gcagacgcag agccaggaga agaaggacgc gctgcgcctg tgcggcgccg acctgcgcga
     2041 agtgcccgcg gccccctaca gcgaccccaa caactatgtg cacgtggccg agcgcctcgc
     2101 ggccgaactc gcgcgcagcg agcccaacgg ggtgctgtac gcgaaccagt gggacaacct
     2161 gtccaaccgc gacggccatg tgcgctccac cggccccgag atctggcagc agaccgacgg
     2221 ccgcatcgac ggcttcacct gcgcgatcgg caccggcggc acgctggccg gcgtggggct
     2281 gtacctgaag cagcacaagc cgggtgtcgt gatcgccgcg gccgacccga tgggcgcggc
     2341 gatctaccac tacgtcaagc atggcgagct gcgcgccgaa ggcagctcga tcaccgaagg
     2401 cataggcgtg ggccgcatca cgcgcaacct ggagggggcg cccatcgacg acgcctacca
     2461 gatcaccgac gaagaagcgt tgcccttgct gttcgacttg ctgcgcgaag aaggcctgtg
     2521 cctgggcggc tccagcggca tcaacgtggc cggcgcgatc cgcatggcgc atgctctcgg
     2581 cccgggccac accatcgtga ccatcctcgc cgacgccggc tcgcgctatc aatccaagct
     2641 gtacaacccg gccttcctgc gcgagaa
//
LOCUS       DASUCY010000420         2710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_186384, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000420 DASUCY010000000
VERSION     DASUCY010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186384"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /gene="pqqE"
                     /locus_tag="VFV25_08385"
     CDS             complement(<1..890)
                     /gene="pqqE"
                     /locus_tag="VFV25_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018149 - peptide cross-linking [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="HEY1393382.1"
                     /translation="MDAAGSVERPGGVRQPGPPLWLLAELTHRCPLHCVFCSNPVDYA
                     SRREELDTRDWLNVLDQGRALGAVQLGLSGGEPLLRDDLEEIVAHGHGLGYYINLITS
                     GVGLTEQRAAALKKAGLDHIQLSFQDSSRELNDFLSSTRTFELKSRVAAIIKSLGYPM
                     VLNCVMHRYNLPHVGRIIAMAEAMGAEFLELANTQYYGWAWLNRDALMPTPTELADAE
                     AIVDCHRERLAGRMRILWVSPDYVDAKPKACMNGWGSVFLLVSPDGTALPCHSARMLP
                     GLNPPSVLQHPLREIWYDSAA"
     gene            complement(868..1131)
                     /gene="pqqD"
                     /locus_tag="VFV25_08390"
     CDS             complement(868..1131)
                     /gene="pqqD"
                     /locus_tag="VFV25_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HEY1393383.1"
                     /translation="MPLLDDRPTVSALYRLQFEPAQNSWVLLYPEGMVKLNASAAEIL
                     KRCDGIASVQELIEGLEREYAGVELREDVCHFLSDAYGRGWIC"
     gene            complement(1131..1889)
                     /gene="pqqC"
                     /locus_tag="VFV25_08395"
     CDS             complement(1131..1889)
                     /gene="pqqC"
                     /locus_tag="VFV25_08395"
                     /EC_number="1.3.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="HEY1393384.1"
                     /translation="MNTDVFQNPVAEAAPWSAEVFEAKLRAKEARYHIHHPFNVRLNR
                     GELQPFQVRGWVANRFYYQICIPVKDAAILSNCPDREVRRRWIDRLLDHDGRGDFQGA
                     NAGGIEAWVRLGEAVGLSREDMWSQEHVVPGVRFAVQAYVNFARKAPWQEAAISSLTE
                     LFAPRIHADRLSTWPSCYPWIEPDGLAYFRSRIPLATRDVEHGLQIAREWCITRERQL
                     RALEILQFKLDILWTMLDAIALAYPDNLPKGDQP"
     gene            complement(1886..>2710)
                     /gene="pqqB"
                     /locus_tag="VFV25_08400"
     CDS             complement(1886..>2710)
                     /gene="pqqB"
                     /locus_tag="VFV25_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="HEY1393385.1"
                     /translation="SAPRTQSSIAISCDGEQWVLLNASPDIGQQIRSNAALHPRGGPR
                     DTPIKAVILMDAQVDHVTGLLSLREGPPIELHCTPCVFEDLTTGLPILPTLEHYSGVH
                     WHLLPVAGDTTVAEFQLEAFPQLRFHAIAISGKAPPYSAHRTDPTVGDNIALHIEDLS
                     SGASLFYAPGLAEVGEAELQWMHASDCLLVDGTFWSEHEMLDAGLGRYAAADMGHLPQ
                     SGPRGMIAALAPLPARRKLLIHINNSNPILDEDSAQRRVLTQHGIEVAHDGMEIEL"
BASE COUNT          441 a          861 c          955 g          453 t
ORIGIN      
        1 gctgcgctgt cgtaccagat ctcgcgcaag gggtgttgga gcaccgaggg cgggttcagg
       61 cccggcagca tgcgcgcgct atggcagggc agggcggtgc cgtctggcga gacgagcagg
      121 aagaccgagc cccagccgtt catgcaggcc ttgggcttgg cgtcgacata gtccggcgag
      181 acccacagga tacgcatgcg cccggccagg cgctcgcggt ggcagtcgac gatggcctct
      241 gcatcggcca gctcggtcgg tgtgggcatc agcgcatcgc ggttgagcca ggcccagccg
      301 tagtactgcg tgtttgccag ctccagaaac tcggcgccca tcgcctcggc catcgcaatg
      361 attcggccca cgtgcggcag gttgtagcgg tgcatcacgc agttgagcac catggggtag
      421 cccaggctct tgatgatggc cgcaacgcgg gacttgagtt cgaaggtgcg cgtggacgag
      481 agaaagtcgt tgagctcgcg cgaggagtcc tggaaggaca gctggatgtg gtccagcccg
      541 gccttcttga gggccgcggc gcgctgctcg gtcaggccga cacccgaagt gatgagattg
      601 atgtaatagc caaggccatg gccatgggcg acgatctctt ccaggtcgtc gcgcagcagc
      661 ggctcgccgc ccgacaggcc gagctgcaca gcgcccagtg cgcggccctg gtccagcaca
      721 ttcagccagt ctcgcgtgtc gagttcttcg cggcggctcg cgtagtcgac cgggttggag
      781 cagaagacgc agtgcagcgg gcagcgatga gtcagctcgg ccagcagcca aagcggcggg
      841 ccgggttgac ggacaccccc ggggcgctca acagatccag ccgcgtccat aggcgtcgct
      901 gaggaaatgg cagacgtcct cgcgcagctc gacgcccgcg tattcgcgct ccagtccttc
      961 gatcagctcc tgcacgctgg cgatgccgtc gcagcgcttc aggatttcgg ccgcgctcgc
     1021 attcagcttc accatgcctt cggggtagag cagaacccag ctgttctgcg ccggctcgaa
     1081 ctgcaggcgg tagagcgcgg agaccgtcgg ccggtcgtcg agcaagggca tcacggctga
     1141 tctcccttgg gcaggttgtc cggataggca agcgcgatgg catccagcat cgtccacagg
     1201 atgtcgagct tgaactgcag gatctccaaa gctcgcagct ggcgctcgcg cgtgatgcac
     1261 cactcgcgcg cgatctgcag gccatgctcc acgtcgcgcg tcgcgagcgg gatgcggctg
     1321 cggaagtagg ccaggccgtc gggctctatc cacggatagc aggagggcca ggtgctgagc
     1381 ctgtccgcgt ggatcctggg agcgaagagc tcggtcagcg aggagatcgc cgcttcctgc
     1441 cacggcgctt tgcgcgcgaa gttcacatag gcctggaccg cgaagcgcac gccgggaacc
     1501 acgtgctctt gcgaccacat gtcctcgcgg ctcaggccca cggcctcgcc cagcctgacc
     1561 caggcctcga tgccgcccgc gttggcgcct tggaagtcgc cgcgcccgtc gtgatcgagc
     1621 aggcggtcga tccagcgccg ccgcacttcg cggtccgggc agttcgacag gatcgccgcg
     1681 tctttgaccg ggatgcagat ctggtagtag aagcgattgg ccacccagcc gcgcacctgg
     1741 aaaggctgca gctcgccgcg gttgaggcgg acattgaagg gatggtggat gtggtagcgc
     1801 gcctccttgg cgcgcaactt ggcctcgaag acctcggccg accaaggggc ggcctcggca
     1861 acggggttct ggaagacgtc ggtgttcaca gctcgatctc catgccgtca tgcgcgacct
     1921 cgatcccgtg ctgcgtcagt acgcgccgct gcgcgctgtc ttcgtcgagg atggggttgc
     1981 tgttgttgat gtggatcagc agcttgcgcc gcgccggcag gggcgcgagc gcggcgatca
     2041 tgccgcgcgg cccgctctgc ggcaggtggc ccatgtcggc ggccgcatag cggccgaggc
     2101 ccgcgtcgag catctcgtgc tcgctccaga aggtgccgtc gacgagcagg cagtccgacg
     2161 cgtgcatcca ttgcagctcg gcctcgccga cctcggccag gcccggcgca tagaacaggc
     2221 tcgcgccact gctcaggtct tcgatgtgca gcgcgatgtt gtcgcccacc gtcgggtcgg
     2281 tgcggtgggc cgaatagggt ggcgccttgc cggagatggc gatggcgtgg aagcgcagct
     2341 gaggaaaggc ctcgagctgg aactcggcga ccgtcgtgtc gcctgccacc ggcagcagat
     2401 gccagtgcac accgctgtaa tgctccagcg tgggcaggat gggcagcccg gtcgtgagat
     2461 cctcgaacac gcagggcgtg cagtgcaatt cgatgggagg accttcgcgc aggctcagca
     2521 ggccggtaac gtggtcgacc tgggcgtcca tcaggatcac cgccttgatc ggcgtatccc
     2581 tggggccacc gcgcggatgc agggcggcat tgctgcggat ctgctggccg atatccggcg
     2641 aggcattgag cagcacccat tgctcgccgt cgcaggaaat ggcgatcgag ctctgcgtgc
     2701 gcggtgccga
//
LOCUS       DASUCY010000421         2618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_73971, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000421 DASUCY010000000
VERSION     DASUCY010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73971"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VFV25_08405"
     CDS             <1..239
                     /locus_tag="VFV25_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008642659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEY1393386.1"
                     /translation="AFTTHGSRPFAQMAWRPGDWLVFGCETRGLAPSLRDGFAPEQRV
                     RLPMREGQRSLNLSNTVAIAVFEAWRQNGYAGGG"
     gene            complement(334..1362)
                     /locus_tag="VFV25_08410"
     CDS             complement(334..1362)
                     /locus_tag="VFV25_08410"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEY1393387.1"
                     /translation="MKITVLGGGAWGTAIAVNAAARHDVLLWARDAGQVAGMRAARRN
                     ERYLPEIELPQALQLGADLDQALAHGEAGLYIIATPMAALEQMLQALPTHGRALWLCK
                     GFQAGTGRLGHEVARAVHAQASTGVLSGPSFALEVARGLPTALVAASEDPHLSELAVQ
                     ALHSESLRIYTSGDPVGVEVGGAVKNVLAIATGICDGATGIAGGIPAGDPSLPPGLGM
                     NARAALITRGLAEMTRLGIALGARAETFMGLSGLGDLVLTATGDLSRNRQVGLRLAQG
                     KSLPQILAELGHVAEGVYSAANVLRRAREKGVEMPITELVVEVIEGRLTVGQAVRALM
                     SREAKPEA"
     gene            complement(1379..1864)
                     /gene="secB"
                     /locus_tag="VFV25_08415"
     CDS             complement(1379..1864)
                     /gene="secB"
                     /locus_tag="VFV25_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_036230692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEY1393388.1"
                     /translation="MAEQQVAGNQPVFQIQRVYLKDMSLEQPNSPQILLEQEAPQVEI
                     NLGLRADGVGEGMFEISVTATVTTKVKDKTLFLVEAKQAGIFEIRNIPAEQLQPILAV
                     ACPQIVYPYLRATVADIVTRAGFPPVHLAEVNFQAMYEAQQAQAAQPNGGGASPVTTN
                     A"
     gene            complement(1967..2233)
                     /gene="grxC"
                     /locus_tag="VFV25_08420"
     CDS             complement(1967..2233)
                     /gene="grxC"
                     /locus_tag="VFV25_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEY1393389.1"
                     /translation="MKSVKMYTTLVCPYCQRAKMLLKQRGVDAIEEVRVDLDPAQRDA
                     MIEITGRRTVPQIFIGNTHVGGCDDLVALDQRGGLMPLLEGAAV"
     gene            complement(2402..>2618)
                     /locus_tag="VFV25_08425"
     CDS             complement(2402..>2618)
                     /locus_tag="VFV25_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEY1393390.1"
                     /translation="ANSRNIPFGSLETAKNLPSNKSLPLVVLCPTGARAGRAAAILRK
                     LGFEKAQALAGGLAAWRDANLPVEKSA"
BASE COUNT          393 a          892 c          911 g          422 t
ORIGIN      
        1 tcgccttcac cacgcacggc agccggccct ttgcgcaaat ggcctggcgg cccggcgact
       61 ggctggtgtt cggctgcgag acgcgcggcc tggcgccctc gctgcgtgac ggctttgcgc
      121 cggagcagcg cgtgcgcctg cctatgcgcg agggccagcg cagcctcaat ctcagcaaca
      181 ccgtggccat cgcggtgttc gaggcctggc ggcaaaacgg ctacgccggc ggcggatgac
      241 agcacccggc cgcggattac cgcgtccacc ctccgtgagg gtgccggcgt gcttgggagc
      301 ggcccggcgc ggcgccggct gtgacgacac ccgtcaggct tcgggtttgg cctcgcggct
      361 catcaaggca cgcacggcct ggcctacggt gagccgaccc tcgatcactt ccaccaccag
      421 ctcggtgatc ggcatctcga cgcccttctc gcgggcgcgc cgcagcacat tggccgcgct
      481 gtagacgccc tcggccacat ggcccagctc ggccaggatt tgcggcagcg acttgccctg
      541 ggccagcctc agacccactt ggcggttgcg cgagaggtcg ccggtggccg tgagcaccag
      601 atcgcccagg ccactcagtc ccatgaaggt ctcggctcgt gcgccgaggg cgatacccag
      661 gcgcgtcatc tcggccaggc cgcgcgtgat cagcgcggcg cgggcgttca tgcccaggcc
      721 gggcggcagc gaaggatcgc cggccgggat gccgccggca atgcccgtgg caccatcgca
      781 gatgccggtg gcgatggcca gcacgttctt taccgcgccc cccacttcga cgccgaccgg
      841 gtcgcccgag gtgtagatgc gcaggctctc gctgtgcaag gcctgcacgg ccagttcgct
      901 caagtgcgga tcttcgctgg ccgccaccaa ggccgtgggc aggccgcgcg ccacttccag
      961 cgcgaagctg ggcccggaca gcacaccggt gctggcctgg gcgtgcacgg cgcgggcgac
     1021 ctcatgcccg agccggccgg tgccggcctg gaagcctttg cacagccata gcgcgcgtcc
     1081 gtgcgtcggc aacgcctgca gcatctgctc cagcgccgcc atgggcgtgg cgatgatgta
     1141 gagccccgcc tcgccatgcg ccagcgcctg gtcgagatcg gcgccgagct gcagcgcctg
     1201 cggcaactcg atctccggca gatagcgctc gttgcggcgc gcggcgcgca tgccggcgac
     1261 ctggccggca tcgcgcgccc acagcagcac gtcatggcgc gcggcggcgt tgacggcgat
     1321 ggcggtgccc caggcgccgc cgccgaggac cgtgatcttc atcgcggccc ggcgccgatc
     1381 aggcgttggt ggtgaccggc gaggcaccgc cgccgttggg ctgggcggcc tgtgcctgct
     1441 gagcctcgta catggcctgg aagttcacct cggccagatg caccggcggg aagccggcgc
     1501 gcgtgacgat gtcggccacg gtggcacgca gatagggata gacaatctgc gggcaggcca
     1561 cggccaggat gggctggagc tgctcggcag ggatgttgcg gatttcgaag atgccggcct
     1621 gcttggcctc gaccaggaac aaggtcttgt ccttcacctt cgtggtcacg gtggcggtca
     1681 cggagatctc gaacatgccc tcgccgacgc catcggcgcg cagtcccaga ttgatctcga
     1741 cctggggcgc ctcctgctcg agcaggatct gcggcgagtt gggctgctcc agcgacatgt
     1801 ccttcagata gacgcgctgg atctggaaca cgggctgatt gcccgcgact tgctgttcgg
     1861 ccatggtgtg aacctcagga gacttcggag gcccgcggct ctgtccagcg agcgcgggcg
     1921 ggaaccgaag attatggcgc agccacgtga cgcgccgaac gctcccctag accgcggcgc
     1981 cttcgagcaa aggcataagg ccgccgcgct ggtccagcgc cacgaggtcg tcgcagccgc
     2041 ccacgtgggt gttgccgatg aagatctgcg ggacggtgcg ccggccggtg atctcgatca
     2101 tggcatcgcg ctgggcagga tcgaggtcga cgcgcacctc ttcgatggcg tcgactccac
     2161 gctgcttgag cagcatcttg gcgcgctggc agtaagggca gaccagcgtg gtgtacatct
     2221 tcacggactt catgcttgga cttcgagcaa tctgcatgcc cgacggatat cgtcagaccg
     2281 acaacgggca gattgcgtgc ggcgccgaca tttcaatgga aaacccgcaa tggcgggcat
     2341 agctgcggca aatggccttg ttcgtctcag tggctccacc ggacttcgtg cgacgagtca
     2401 ttcaagctga cttttcgact gggagatttg cgtcgcgcca ggccgccagg ccgcccgcca
     2461 gggcttgcgc cttctcgaag ccgagcttgc gcaggatggc cgcggcccgg cctgcgcgcg
     2521 ccccggtggg gcacagcacc accagcggca gggacttgtt gctcggcagg ttcttcgcgg
     2581 tctcaagact gccgaacggg atgttgcgcg aattggcg
//
LOCUS       DASUCY010000422         4537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_459493, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000422 DASUCY010000000
VERSION     DASUCY010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459493"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VFV25_08430"
     CDS             complement(<1..218)
                     /locus_tag="VFV25_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible membrane protein A"
                     /protein_id="HEY1393391.1"
                     /translation="MRTAEAVPDVLACEQCDLLHRYERGKTEGFTPRCRRCDAPLMRP
                     AGPPPDLPLALALTSLAALAVAHTNPILG"
     gene            complement(245..907)
                     /locus_tag="VFV25_08435"
     CDS             complement(245..907)
                     /locus_tag="VFV25_08435"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HEY1393392.1"
                     /translation="MRPLQALPLRSLCAAVAVMALSACSTPVDHVHTLLPTAATTSEQ
                     RAEEPQAPRWLALQVIVPEALDRETWLLRGPGQQIEVHEHQRWPQPLSAELAQALARR
                     LDAQLAAEGWSVVPADEMPGAKRRLVVRWLAFDAWMHPTPGVHDTWLWAIECPRRRAG
                     SPALLASGRDDYLTAGSSPSAPDAFDRVAEEHAQALQQATLAIAAAWRAQHAGDDQRC
                     SE"
     gene            complement(904..2595)
                     /locus_tag="VFV25_08440"
     CDS             complement(904..2595)
                     /locus_tag="VFV25_08440"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEY1393393.1"
                     /translation="MSDTPPPSIASDANDARDDLPVPETAPRANVSLWLIWLIPLLAV
                     AVGLGLAVKAVTDHGPTITIYFHNGDGLEAGKTHIKLRDVDVGLVKHVRLSPDLREVE
                     VTAELNKGTAAERLLRRDTRFWVVRPRVGVGGVSGLGTLFSGSYIGVDAGRSDESATD
                     FRGLDVQPPITADVPGRSFALLADDLGSLDIGSPVYFRRVPVGQVSSYTLDAKGQRIG
                     LSVFVAAPYDRFVTPHSRFWESSGVDLSFDANGLRLETQSVASIIAGGIAFGDGPVST
                     DAPATEAADAEAPEGTQYRLYARRYEAMKQPDGPGFDYRLLLNGSVRGLSVGAPVEYR
                     GLPIGEVTRISVEGTVGRRSPEPRIAVDLRVHPSRLPTVEGQRVDDAGGAQQRARLDP
                     MVAHGFRAQLRTGNLLTGQLFVALDFFPHSPAAHIDWRANPPVMPAIPSSLDDLQETL
                     GSLVHKLDQLPLDTLAEDAHRDLVALHADLQRADELLAHVDRDVTPALAQALKDAHAA
                     MDDLKQVLGTADSTLTSSTQAIGEVQKAAKALRSLADTLERHPESLLRGKSEDGR"
     gene            complement(2588..3208)
                     /locus_tag="VFV25_08445"
     CDS             complement(2588..3208)
                     /locus_tag="VFV25_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEY1393394.1"
                     /translation="MNLRSGSALQLGLWRCHACATLNRPASGAGPTTWLCGHCGTVLF
                     ARRPDSIGRAWAFLIAATALYIPANLMPVTSTASLLAAQSDTLFSGILFLWDGGSWAL
                     AAIVFIASILIPSAKLIVLSYLLLSVQRRSRHARLLRLRLYRVLELVGRWSMLDVFVV
                     TMLAALVRIQSLAQLQPGPGAIAFGAVVVLTMLATRSFDPRLIFDE"
     gene            complement(3288..3551)
                     /locus_tag="VFV25_08450"
     CDS             complement(3288..3551)
                     /locus_tag="VFV25_08450"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY1393395.1"
                     /translation="MNDSDFSEERIAYALRLTEGGAPVVDVCWDIGISQATFNTWREK
                     YGNRGASELQEKLRMLEDENARLRRRVADLTLAGCASAWYFMP"
     gene            complement(3661..>4537)
                     /locus_tag="VFV25_08455"
     CDS             complement(3661..>4537)
                     /locus_tag="VFV25_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HEY1393396.1"
                     /translation="VRAPLMLQVVLGLDAATIASAFLVAPAAMGQRLARAKTKIRQAG
                     IAFEVPERGLLRERLDAVLEAIYAAYAEGWSDPQGADARRRNLAEEAIWLGRLAASLL
                     PDEGEALGLLSLMLHAHARRDARRDTAGDYVPLAQQDPALWDSKLIDEAEALLAQANA
                     CAEPAGRFHLEAAVQSAHAVRRHGGDSDWAAIERLYDALCALTGSPVAALNRAVALSY
                     MRGMAAALSALQALEHDTRLADYQPYWAARAELLARSGNAAASAQAYQQAIGLERDSA
                     VRRFLQARADALPST"
BASE COUNT          696 a         1543 c         1614 g          684 t
ORIGIN      
        1 ccgaggatgg ggttcgtgtg cgccacggcc agggcagcta gcgaagtgag cgcgagggcc
       61 agcggcaggt ctggcggcgg tcctgcgggg cgcatcagcg gcgcgtcgca gcggcggcag
      121 cgcggcgtga acccctcggt cttgccgcgc tcgtagcggt gcaacagatc gcattgttcg
      181 caggccagca catcgggcac cgcttcggcg gtgcgcacgg gaagggtggt gaccagcttt
      241 ctcctcattc ggagcaccgt tgatcgtcac cggcgtgctg cgcgcgccaa gcggctgcga
      301 tggccagtgt ggcctgctgc agcgcctggg catgttcctc ggcgacgcgg tcgaaggcat
      361 cgggcgcgct cggcgacgaa ccggccgtga ggtagtcatc gcgaccgctg gccagcagcg
      421 cgggcgatcc cgcgcggcgc cgcgggcatt cgatagccca tagccaggtg tcgtgcacgc
      481 caggcgtcgg gtgcatccac gcgtcgaagg ccagccagcg cactacgaga cggcgcttgg
      541 cgcccggcat ctcgtcggcc ggcaccacgc tccagccctc ggccgcgagc tgcgcatcga
      601 gccgccgcgc cagcgcctgc gccagttcgg ccgacaaggg ctggggccag cgctggtgct
      661 cgtgcacttc gatttgttgt cccgggccgc gcagcagcca ggtttcgcgg tccagcgcct
      721 cgggcacgat cacctgcagc gcgagccagc ggggcgcttg cggttcctcg gcgcgctgct
      781 cggaggtggt ggccgccgtg ggcagcaaag tgtgaacatg atcgaccggc gtcgagcagg
      841 ccgacaaggc catcacggcg acggcggcac acagcgatcg caagggcagg gcctgaagcg
      901 gtctcatcgt ccgtcctccg atttgccgcg cagcagcgat tcgggatgcc gctccagtgt
      961 gtcggccaag ctgcgcaagg ccttggcggc cttctgcacc tcgccgatcg cctgcgtcga
     1021 gcttgtgagc gtgctgtccg cggtgcccag cacctgcttc aggtcgtcca ttgcggcatg
     1081 ggcgtccttc agcgcctgcg cgagcgcggg agtcacgtcg cggtcgacgt gggcgaggag
     1141 ttcgtcagcc cgctgcaggt ccgcatgcaa ggcaacgagg tcgcggtgtg catcctccgc
     1201 cagcgtgtcc agcggcagct ggtcgagctt gtgcaccagg ctgcctagcg tctcttgcag
     1261 atcgtccagg ctgctcggaa tggcgggcat gaccggcggg ttggcgcgcc agtcgatgtg
     1321 cgccgccggg ctgtgcggga agaagtccag cgcgacaaag agctggccgg tcagcaggtt
     1381 gccggtccgc agttgcgctc gaaagccgtg ggcgaccatc gggtccaggc gggcacgctg
     1441 ctgcgcgccg ccggcgtcgt cgacgcgctg gccctcgacg gtgggcaggc gcgacgggtg
     1501 gacgcgcagg tcgacggcaa tgcgcggctc cgggctgcgg cggcccacgg tgccttcgac
     1561 cgagatgcgc gtgacctcgc cgatcggcag tccgcggtac tccaccgggg cgccgaccga
     1621 gagcccgcgc accgatccgt tgagcagcag gcggtagtcg aagccggggc cgtcgggctg
     1681 cttcatggcc tcgtatcggc gcgcgtagag tcggtactgc gtgccttccg gcgcttcggc
     1741 gtcggccgcc tcagtggccg gcgcgtcggt gctcaccggg ccgtcgccga acgcgatgcc
     1801 gccggcgatg atgctggcca ccgactgtgt ctccagccgc aagccgttgg catcgaagga
     1861 caggtcgacg ccgctcgatt cccagaagcg gctgtgcggc gtgacgaagc ggtcgtaggg
     1921 cgccgcgacg aagaccgaca ggccgatgcg ctggcccttg gcgtccagcg tgtagctgct
     1981 gacctggccc accggcacgc ggcggaagta cacgggcgac ccgatgtcca gagatccgag
     2041 atcgtcggcc agcagcgcga agctgcgccc cggcacgtcg gccgtgatcg gcggctgcac
     2101 gtccaggccg cggaagtcgg tggcgctctc atccgatcgg cccgcgtcca cgccgatgta
     2161 cgaaccggag aacaaggtgc ccagccccga cacgccgccc acgcccacgc gtggccgcac
     2221 cacccagaag cgcgtgtcac ggcgcagcag gcgttcggcc gccgttccct tgttcagctc
     2281 cgcggttacc tcgacctcgc gcaagtcggg gctcaggcgc acgtgcttga ccaggcctac
     2341 gtcgacgtca cgcagcttga tgtgtgtctt gccggcctcc aggccgtcgc cgttgtggaa
     2401 gtagatggtg atcgtcgggc cgtggtcggt cacggccttg acggcgaggc caaggccgac
     2461 ggcgactgcg agcagcggga tcaaccagat cagccacagc gagacgttgg cgcgcggcgc
     2521 ggtctccggt acgggcagat cgtcgcgcgc atcgtttgcg tcggatgcga tcgagggcgg
     2581 gggcgtgtca ctcatcgaag atcagccgag ggtcgaagct gcgggtggcg agcatggtca
     2641 gcacgacgac ggcgccaaag gcgatcgcgc cgggacccgg ctgcaactgc gccaggctct
     2701 ggatccgcac cagggccgcg agcatggtga cgacgaagac gtcgagcatc gaccagcggc
     2761 ccaccagctc cagcacgcgg tacaggcgca ggcgcagcag ccgtgcatgg cgcgaacgac
     2821 gctgcaccga cagcagcagg taggacagca cgatcagctt ggcgctggga atgagaatgc
     2881 tggcgatgaa gacgatggcc gccaaggccc aggagcctcc atcccacagg aacaggatgc
     2941 cgctgaagag ggtgtcactc tgcgcggcga gcaacgacgc cgttgaggtg actggcatca
     3001 ggttggccgg gatgtacagc gccgtggccg cgatcaggaa cgcccacgca cgtccgatgc
     3061 tgtccggccg gcgcgcgaag aggacggtgc cgcaatgtcc gcagagccag gtggtgggcc
     3121 ccgcacccga ggccgggcgg ttgagcgtgg cgcaggcgtg gcagcgccaa aggccgagct
     3181 gcaatgcgga gccgctgcgg aggttcatgg ctcgcagcat gccatggcgc cgccttgctt
     3241 gctacttgcc gcctggcacg ccagccggtg tcggccgccc tacccgctca tggcatgaaa
     3301 taccaggcag atgcgcaccc ggccagcgtc agatcagcca ccctgcgccg cagccttgca
     3361 ttctcatcct cgagcatcct cagcttctct tgcagttcag acgcaccccg gttgccgtac
     3421 ttctccctcc aagtgttgaa cgtggcctgg gaaatgccaa tgtcccagca cacatcgacc
     3481 accggtgcgc cgccctccgt caatcgaagg gcgtaggcga tccgttcttc gctgaagtcg
     3541 gagtcgttca tcggttctcc agtcttgctc gcggcgtccc agccatctcc actgacctgg
     3601 gcggtcgcgc atgtcctagc gttggggtgg acgcgcctca tgtcaggggc gggacgatgc
     3661 ctatgtcgac ggcaatgcgt cggcacgtgc ctgtaggaag cggcgcacgg ccgaatcgcg
     3721 ttccagaccg atggcctgtt ggtaggcctg cgcggaggcc gccgcgttgc cgctgcgcgc
     3781 gagcagctcg gcacgcgcag cccagtaggg ctggtagtcg gccagacgcg tgtcgtgctc
     3841 gagtgcctgc agcgccgata gagcggccgc catgccgcgc atatagctca gcgcgactgc
     3901 gcgattgagc gctgccaccg gcgaaccggt gagggcgcat agcgcgtcgt acagccgctc
     3961 gatcgctgcc caatcgctgt cgccgccgtg gcgccgcacc gcgtgtgccg actgcaccgc
     4021 ggcttcgaga tggaagcgcc cagccggttc ggcacaggca ttggcctgcg ccagcagcgc
     4081 ctcggcctcg tcgatcagct tggagtccca aagcgccgga tcttgctgcg cgagcggcac
     4141 atagtcgcct gcggtgtcgc ggcgcgcgtc gcgcctggca tgggcatgca gcatgagcga
     4201 cagcaagccc aaggcctcgc cctcgtcggg cagcagcgaa gcggccagac gcccaagcca
     4261 gatcgcttcc tcggccagat tgcgtcgacg cgcgtcggcg ccctgcgggt cggaccagcc
     4321 ctcggcatac gcggcgtaga tggcctccag caccgcatcc aggcgttcgc gcagcagccc
     4381 gcgttccggc acctcgaagg caatgccggc ctgccggatc ttggtcttcg cgcgggcaag
     4441 ccgctggccc atcgccgctg gcgccaccag aaacgccgat gcgatcgtgg ccgcatcgag
     4501 gccgagcacc acctgcagca tcagtggtgc gcgcacg
//
LOCUS       DASUCY010000423         3363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_748385, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000423 DASUCY010000000
VERSION     DASUCY010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748385"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VFV25_08460"
     CDS             <1..525
                     /locus_tag="VFV25_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HEY1393397.1"
                     /translation="DPVHKISIIPRGIGALGYTLQRPTEDRYLATRSELENRLAVLLG
                     GRAAEALAIGELSTGAADDLAKASELALDMVTRFGMSEEVGQVAYERPRSPFLDTTGQ
                     LHAERAHSEATARAIDEAVRRLIDAAFARASEILTARRALLDEGARMLLANETLTEAE
                     LGPLAERARATRGS"
     gene            complement(520..1959)
                     /locus_tag="VFV25_08465"
     CDS             complement(520..1959)
                     /locus_tag="VFV25_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY1393398.1"
                     /translation="MFTLDRRSAVPLAAQIEQQLRLLLERGQLAAGARLPSIRRLAQQ
                     LGVSPNTVVVAYDRLVAEAALEARGKAGYFVSPWVHGGAETAALLEAGDEQDPAWLAQ
                     QSNDQPPGLLLASSSALPASWLQDAVPAAVVHRAFAALPGGMATRCPPQGLPALRERL
                     ALLMRGQGIPVDASRVMTTQGASQAIDVICRAFVQPGDAVLVENPGYHLLPVRLAQAG
                     ARVLSVARGADGVDLVQLETLFAEHRPRLFFMQNTLHNPTGWTAPPANLHRVLTLAER
                     HGVLIAEDDVYGHLLPGQGTRLAQLSSLSRVIYYASFCKVLSPTLRMGYVAADPALLK
                     PMLRQKISASLTGSALNEGILLELLATGRVRKHFDRLQGQLASARNTCMRLLRAAGIT
                     FESPGEAGLFLWGRVPEGVDLDLLVRDAWRQGILLAGGTTFSPVGPPAACLRFNVVLS
                     QHVRLTRYLEQRLEALAQGQGTLQRLARS"
     gene            complement(1989..2204)
                     /locus_tag="VFV25_08470"
     CDS             complement(1989..2204)
                     /locus_tag="VFV25_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393399.1"
                     /translation="MQRTAALNPQHAVAHATARLGAWLRHAWDELMLDADERFLRGAR
                     DLADLEWRLRRLERGRADRFAPLHPDA"
     gene            2469..>3363
                     /locus_tag="VFV25_08475"
     CDS             2469..>3363
                     /locus_tag="VFV25_08475"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY1393400.1"
                     /translation="MRTADIAKFFFRLAVVIVAAASPGVRAADGPSKQIPARIELYSI
                     QTLTLTDQQFLTGDAAAGKPVTITGELRIAQGAGRLPVVVLQHGSGGMGPNVDMWARE
                     LNQIGVSTFALDGFTGRGLTEVNTNQALLGRLNFILDIYKALEILAKHPRVDPNRIVL
                     MGFSRGGQATLYASLVRFQRLWNKSGVEFAGYLPFYPDCMTSYRSDADEVDRPIRIFG
                     GTPDDYSPIASCKSYVERLRGAGHDVAVTEYPNAAHAFDNPLGAQPAAVIAKAQTARN
                     CRIREDADGVLTDLATQQPFSY"
BASE COUNT          564 a         1151 c         1137 g          511 t
ORIGIN      
        1 gaccccgtgc acaagatctc gatcatcccg cgcggcatcg gcgccctggg ctacacgctg
       61 cagcggccca ccgaagaccg ctacctcgcc acgcgcagcg agctcgagaa ccggctggcc
      121 gtgctgctcg gcggccgcgc agccgaggcg ctggcgatcg gcgagctctc caccggcgcg
      181 gccgacgacc tggccaaggc cagcgagctg gcgctggaca tggtcacgcg cttcggcatg
      241 agcgaagagg tcggccaggt cgcctacgag cggccgcgca gcccgttcct cgacacaacc
      301 ggccagctgc acgccgagcg agcgcacagc gaggccaccg cgcgcgcgat cgacgaagcg
      361 gtgcgccgct tgatcgatgc cgccttcgca cgcgccagcg agatcctcac cgcgcggcgc
      421 gcgctgctcg acgagggcgc gcgaatgctg ctggccaacg agacgctgac cgaggcagag
      481 ctcgggccgc tggccgagcg tgcacgcgct acgcggggct catgaccgcg ccaatcgctg
      541 caaggtgccc tgcccctgcg ccaacgcctc cagccgctgc tcgagataac gcgtcagccg
      601 cacgtgctgg ctcagcacga cattgaatcg caggcaggct gccggggggc ccacggggct
      661 gaaggtggtg ccgccggcga gcaggatgcc ttgccgccag gcgtccctga ccagcaggtc
      721 gagatcgacg ccttccggca cacggcccca gagaaacagc cctgcctcgc ccgggctttc
      781 gaacgtgata ccggccgcac gcaagagccg catgcaggtg ttgcgcgccg aggcgagctg
      841 gccttgcagg cggtcgaagt gcttgcgcac acggccggtg gccagcagct ccaggaggat
      901 cccctcgttc agcgccgagc cggtgagcga tgcagagatc ttctgccgga gcatcggctt
      961 cagcagggcc gggtcggccg ccacgtagcc catgcgcagc gtcgggctca acaccttgca
     1021 gaagctggcg tagtagatga ccctggacag gctggacagc tgcgcaagcc gcgtgccctg
     1081 gcccggcaac aggtggccgt agacatcgtc ttcggcgatc agcacgccgt gccgttcggc
     1141 cagagtcagc acgcggtgca ggttggcggg cggcgccgtc cagcccgtgg ggttgtgcag
     1201 cgtgttctgc atgaagaaca ggcgcggtcg gtgctcggcg aacagcgtct ccagctgcac
     1261 caggtctacc ccatcggcgc cccgcgcgac cgacagcacc cgcgcaccgg cctgcgccag
     1321 tcgcacgggc agcaggtggt agcccgggtt ctcgaccagc accgcgtcac ccggctgcac
     1381 gaaggcgcgg caaatcacgt caatggcctg ggaggccccc tgcgtggtca tcacccggct
     1441 ggcgtcgacg ggaatgccct ggccgcgcat cagcaacgcc agccgctcgc gcagggccgg
     1501 caggccctgg ggagggcagc gcgtggccat gccgcccggc agcgcggcga aggcacggtg
     1561 gaccacggcg gcgggcacgg cgtcctgcag ccagcttgcc ggcaatgcgc tgctgctggc
     1621 cagcagcagg ccgggcggct gatcgttgga ctgctgtgcg agccaagccg ggtcctgctc
     1681 gtcaccggcc tccagcagcg ccgctgtctc ggctccgccg tgcacccagg gtgagacgaa
     1741 gtagccggcc ttgcctcgcg cttccagcgc tgcctcggca accagccggt cgtaggcgac
     1801 gaccacggtg ttcgggctga caccgagctg ctgcgccagc cgtcggatcg agggcagtcg
     1861 cgcacccgcc gcgagttgcc cgcgctcgag cagcagccgc aactgctgct cgatctgcgc
     1921 ggccagtggc acggccgatc ggcggtccag ggtgaacaaa cgggtgcctc ttctcgatga
     1981 cgcgattctc acgcatcggg gtgaagcggc gcgaagcgat cggcgcgacc acgctccagg
     2041 cgtcgcagcc gccattccag gtcggccaga tcgcgggctc cgcggaggaa gcgctcgtcg
     2101 gcatccagca tgagctcgtc ccacgcgtgc cgcagccatg cacccaagcg tgcggtggca
     2161 tgcgcgacgg catgttgcgg attcaaggcg gcggtgcgtt gcatggagtg ctccttcatg
     2221 aggcgatggg ccacgatagg cacggtccac gctgctgcac agccacagaa ggtgcgcaag
     2281 ttgactcgcc tgtatcagcg tcgccgctga cacaggggca gcgaggctag ccgccatcgg
     2341 ccatattcgg actcccatgc cagtccgcaa acaagtaacc tatgaagttg tctgcgagac
     2401 tctgtgcttg acgtcggtgg cgtgagcttg ccgaatgcat ttcgtggcga gaggcccagg
     2461 agggaccgat gagaacagcg gacatcgcaa agttcttctt tcgacttgca gttgtcatcg
     2521 tcgcagccgc aagccccggc gtccgggcgg ccgatgggcc ttccaagcag atcccggcgc
     2581 gcatcgagct gtattccatc cagactctga ctctgacgga tcagcaattc ctgacaggtg
     2641 acgccgcggc cggcaagccc gtgaccatca ccggtgagtt gcgcattgcg cagggggcgg
     2701 gtcgcctgcc cgtagtggtg ctccagcatg ggtcgggtgg catgggcccc aatgtcgaca
     2761 tgtgggctag ggagctgaac cagatcggcg tctcgacctt cgcgctcgac ggcttcacgg
     2821 ggcgcggtct tacggaggtc aataccaatc aggccttgct cgggcgcctg aatttcattc
     2881 tggacatcta caaggcactg gagattctcg ccaagcaccc gcgtgtcgac ccgaatcgca
     2941 tcgtcttgat gggcttctcg cgtggtggtc aagcgacgct ctatgcgagc ctggtccggt
     3001 ttcagcgcct gtggaataaa tcgggcgtcg agttcgcagg ctatctgccg ttctacccgg
     3061 actgcatgac gagctatcgg tccgacgccg acgaggtcga tcgcccgatc cgcatcttcg
     3121 gcgggacgcc ggacgactac agccccattg cgtcgtgcaa gtcgtatgtt gaacgactac
     3181 gcggcgcagg gcatgacgtc gcagtgaccg agtaccccaa cgccgcacac gccttcgaca
     3241 accctctcgg cgctcagccc gcggcggtga ttgccaaggc tcaaacggcg cgcaactgcc
     3301 gaatccgcga ggacgccgat ggggtgctca cggacttggc gacccagcag ccgttttcct
     3361 aca
//
LOCUS       DASUCY010000424         4347 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_330903, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000424 DASUCY010000000
VERSION     DASUCY010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4347
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330903"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1168)
                     /gene="fliD"
                     /locus_tag="VFV25_08480"
     CDS             complement(<1..1168)
                     /gene="fliD"
                     /locus_tag="VFV25_08480"
                     /inference="COORDINATES: protein motif:HMM:NF018852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HEY1393401.1"
                     /translation="MAITSSATASLSSPGIGSGLDVNSIVSKLVSIESQPLNDLQTAA
                     SKIQTSISTYGQLQSLVSSLQTAAQSLGNPSLWKQTSATSTNAAVGVTASTGSAAAGN
                     YTVSVASLAQAQNLASTAYMDSTAQVGSGTLAIQIGTWGSGNSFTPKSGSSTVNLTIA
                     AGSTLANIRDQINAANAGVTASIVTDATGARLTISSANTGLDNQLRITATDSNGAALP
                     PGAGLGALAYAPDVGGGGLCQTQAATNAQATINGLAIQSDSNTLSNVLSGVTLQLNSA
                     TSSPATVTVAPNTGAASTAINSFVSAYNALNSFLTTQTAYNAATKTAAPLQGDSTAIG
                     LKFSMRTLLMGNGGGSSVFARLGDVGFSTAKDGTLSVDSTKLTAALANMPEMAKL"
     gene            complement(1278..2135)
                     /locus_tag="VFV25_08485"
     CDS             complement(1278..2135)
                     /locus_tag="VFV25_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin domain-containing protein"
                     /protein_id="HEY1393402.1"
                     /translation="MPQTINTNIISLDAQRNLNSSQSSLAISMQRLSSGLRVNSAKDD
                     AAGLAIATRMDSQVRGMNVAIRNANDGISLAQTADSALGSVTNALQRMRELAVQAANS
                     TNSGSDRDSLNQEFQQLGSEIQRVLGGTSFNGATILGGSAGALTFQIGANTTANDTIT
                     VTTTNMTTNASITAVTTGGANSSIDSTVNAAGVSSVITALDGAINTINSQRAVFGATQ
                     SRFESIITNLQSASENQTAARSRIMDADFAAETANLSRAQVLQQAGNAMVAQANQLPQ
                     QVLTLLKGG"
     gene            complement(2485..3336)
                     /locus_tag="VFV25_08490"
     CDS             complement(2485..3336)
                     /locus_tag="VFV25_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEY1393403.1"
                     /translation="MPSTINTNLLSLDAQRNLSTSQSALAVSMQRLSSGLRVNSAKDD
                     AAGLAIATRMDAQVRGMNVAIRNANDGISLAQTADSALGSVTNALQRMRELAVQGSNS
                     TNSGSDRDSLNQEFQQLGSEIQRVLGGTTFNGATILGGSAGALTFQVGANTTANDTIT
                     VTTTNMTTNASIAAVTTGANNKIDSTVSAANLANVITALDGAIDTVNSQRATFGATQS
                     RFDAVISNLQSASENQTAARSRIMDADFAAETANLSRAQVLQQAGNAMVAQANQSPNS
                     VLSLLPR"
     gene            3721..>4347
                     /locus_tag="VFV25_08495"
     CDS             3721..>4347
                     /locus_tag="VFV25_08495"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY1393404.1"
                     /translation="MRSTTTNHTSAARQAGQHWQRGRQLGASGRWQDAARAFEAATRS
                     APHDALYWINLAHAHHRCSAHEQAIAAGRRALAIEPGSAVARRLVGESLAAQHRWSEA
                     AACLAELPAGEARDLDYLRELGLARHQANQFKGAIEAYFQALALKIDDALTHYRLGLS
                     FWRLDLKQEACECFRTVLALGLPGLEIGVQGLLSFFERESCRWDLAQQH"
BASE COUNT          623 a         1381 c         1544 g          799 t
ORIGIN      
        1 acagcttggc catttccggc atgttggcga gcgccgccgt cagcttggtg ctgtcgacgc
       61 tgagcgtgcc gtccttcgcg gtcgagaagc ccacgtcgcc gagcctggcg aacacgctcg
      121 agcccccgcc gttgcccatc agcagcgtgc gcatgctgaa cttcaggccg atcgccgtgc
      181 tgtcgccctg cagcggggcg gccgtcttgg tggcggcgtt gtaggccgtc tgcgtggtca
      241 ggaagctgtt gagcgcgttg taggccgaga cgaaggagtt gatcgcggtg ctcgcggcgc
      301 cggtgttggg cgccacggtc acggtggcgg ggctgctcgt cgcgctgttg agctgcagcg
      361 tcacgccgct cagcacattg ctgagcgtgt tgctgtccga ctggatggcc aggccgttga
      421 tggtggcctg cgcgttggtc gccgcctggg tctggcacag gccgccgccg cccacgtcgg
      481 gcgcataggc cagggcgccc agcccggcgc cgggtggcag cgctgcgccg ttcgaatcgg
      541 tggcggtgat gcggagctgg ttgtccaggc cggtattggc cgagctgatt gtcaaccggg
      601 ctccggtggc atcggtcacg atcgaggccg tgacgccggc gttggcggca ttgatctggt
      661 cgcggatgtt cgcgagcgtg gagccggcgg cgatcgtgag gttgacggtg ctggacccgc
      721 tcttgggcgt gaagctgttg ccggagcccc aggtgccgat ctggatcgcc agcgttccgc
      781 tgccgacctg tgccgtggag tccatgtagg ccgtcgacgc caggttctgc gcctgcgcca
      841 ggctggcgac gctgaccgtg taattgcctg cggcggccga gccggtgctg gccgtgacac
      901 ccacggccgc attggtcgag gtcgccgagg tctgcttcca cagcgagggg ttgcccaggc
      961 tctgggccgc ggtctgcagc gaggaaacca ggctctgcag ctggccgtag gtcgagatcg
     1021 aagtctggat cttgctcgcc gcggtctgca aatcgttgag cggctgcgac tcgatggaca
     1081 ccagcttgga cacgatgctg ttgacgtcga gcccgctgcc aatcccgggc gagctgaggg
     1141 acgccgtggc gctggaggtg atggccatga tgactccggt ggtaggcctg tatcggcagc
     1201 ttcctcaaga acttgagccc cgcagcaccg aaaaagtggg gcttaggggc tcggctggga
     1261 atgcagccag atgcggctca accgcccttg agcaatgtca ggacctgctg cggcagctgg
     1321 ttggcctggg ccaccatcgc gttgccggcc tgctgcagca cctgggcgcg cgacaggttg
     1381 gcggtttccg ccgcgaagtc cgcatccatg atgcggctgc gggcagcggt ctggttctcc
     1441 gaggcgctct gcagattggt gatgatggac tcgaagcgcg actgcgtggc gccgaagacg
     1501 gcgcgctggc tgttgatcgt gttgatcgcg ccgtccagcg ccgtgatgac ggagctcacg
     1561 cccgcagcgt tcaccgtgct gtcgatcgag ctgttggcgc cgccggtcgt gaccgccgtg
     1621 atgctggcat tggtggtcat gttggtggtc gtgaccgtga tcgtgtcgtt ggccgtggtg
     1681 ttggcgccga tctggaaggt cagggcgccg gcgctgccgc ccaggatggt cgcgccgttg
     1741 aagctcgtgc cgcccagcac gcgctggatt tccgagccga gctgttggaa ctcctggttc
     1801 agcgagtcgc gatcgctgcc gctgttggtg gagttggccg cctgcacggc cagctcgcgc
     1861 atgcgctgca gcgcgttggt caccgagccc agagccgagt cggccgtctg ggccagcgag
     1921 atgccgtcgt tggcgttgcg gatggccacg ttcatgccgc gcacttgcga atccatgcgg
     1981 gtggcgatgg ccaggccggc ggcgtcgtcc ttggcagagt tcacgcgcag gccggaagac
     2041 aggcgctgca tcgagatcgc cagcgaactc tgcgaagagt tcaggttgcg ctgggcatcc
     2101 agcgaaatta tgttggtgtt gatcgtttgg ggcatttcaa gcactcctca agaagccgtt
     2161 gactcccggc ggatgcgccg gcctgcggat gccagactcc ctgaggaggc ttcatcctgt
     2221 ccagcccggc acgcgtaacc ggcggcggga tcgatggctt ctttttttgc caagcgccct
     2281 ttccgtcagt gctccggacc tcaagcggga atggcccacc tgatgcgacg tattcttcgc
     2341 gaggcttgag ccgcccaatg cggtgaaatg aacccgaaat ccggcgcagt tgcggcctca
     2401 aaaatggaaa gccgccgcat ttgcgtgcgg cggccgtagc agtgcccggc tcggcgtgcg
     2461 ccgggccgaa acgcctcccc ggactcagcg cggcagcagc gagagcaccg agttcgggct
     2521 ctggttggcc tgggccacca tcgcgttgcc ggcctgctgc agcacctggg cgcgcgacag
     2581 gttggcggtt tccgccgcga agtccgcgtc catgatgcgg ctgcgcgcgg cggtctggtt
     2641 ctccgatgcg ctctgcaggt tggagatcac ggcgtcgaag cgcgactgcg tggcaccgaa
     2701 ggtggcgcgc tggctgttga ccgtgtcgat cgcgccgtcc agcgccgtga tcacattggc
     2761 caggttggcc gcgctcaccg tgctgtcgat cttgttgttg gcaccggtgg tgaccgccgc
     2821 gatgctggca ttggtggtca tgttggtggt cgtgaccgtg atcgtgtcgt tggccgtcgt
     2881 gttggcgccg acctggaagg tcagggcgcc ggcgctgccg cccaggatgg tcgcaccgtt
     2941 gaaggtcgtg ccgcccagca cgcgctggat ttccgagccc agctgctgaa actcctggtt
     3001 cagcgaatcg cggtcgctgc cgctgttggt ggagttgctg ccctgcaccg ccagttcgcg
     3061 catgcgctgc agcgcgttgg tgaccgagcc gagcgccgag tcggccgtct gggccagcga
     3121 gatgccgtcg ttggcattgc ggatggccac gttcatgcca cgcacctggg cgtccatgcg
     3181 ggtggcgatg gccaggccag cggcgtcgtc cttggcggag ttcacgcgca ggccggagga
     3241 caggcgctgc atcgagacgg ccagcgcgct ttgcgaagtg ctgaggttgc gctgagcgtc
     3301 caaagacagc agattggtat tgatggtcga aggcatgttg ctctcctcta gatccaaaaa
     3361 agtcgcggca aagtgccgac gtaatcacac gatgtcggtg ggtggtggtg acccaccgca
     3421 ccgtcgattg ccgccgccgg gttgcccctg cattggcgcc cgtccgacac cggacaaccg
     3481 ctgccttcgt cgcccttgca ggctgggaag gagggaggcc aaccggaccg cggatccgtt
     3541 tccagaatcc gttggaaggc ttatcggacg ccgatgcgcc gggctttagg gccggcgatg
     3601 aagttttttc ggacaccccg gggattgcca gaacaaggcg gcgtaaggcc cgcacttccg
     3661 cgcatcgcag gccgggccct ctcaatagac tacgcccagc ctccagccct gcgagctttg
     3721 atgcgatcaa ccaccacaaa ccacacgagc gccgcgcgcc aggccggcca gcattggcag
     3781 cgcggccggc aactgggtgc atcggggcgg tggcaggacg ccgcgcgcgc cttcgaggcc
     3841 gccacgcgca gcgcgccgca cgacgcgctg tattggatca acctggcgca tgcccaccat
     3901 cgctgcagcg cgcatgagca ggccatcgcc gccggccggc gcgcgctcgc catcgagccc
     3961 ggctcggccg tcgcgcggcg cctggtcggc gagagcctgg ccgcgcagca ccgctggtcc
     4021 gaagcggccg cctgcctggc cgagctgccc gccggagaag cccgcgacct cgactacctg
     4081 cgcgaactcg gcctggcgcg ccatcaggcc aaccaattca agggcgcgat cgaagcttac
     4141 ttccaggccc tggcactgaa gatcgacgac gcgctgacgc actatcgcct cggcctgtcg
     4201 ttctggcgcc tcgatctcaa gcaggaagcc tgcgagtgct tccgcacggt gctggccttg
     4261 ggcctgcccg gcctcgagat cggcgtgcag ggcctgctga gcttcttcga gcgcgagtcc
     4321 tgccgctggg atctcgcaca gcagcac
//
LOCUS       DASUCY010000425         3227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_9769, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000425 DASUCY010000000
VERSION     DASUCY010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9769"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2645)
                     /locus_tag="VFV25_08500"
     CDS             complement(<1..2645)
                     /locus_tag="VFV25_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEY1393405.1"
                     /translation="MAQRPDYIYSGGNVVMHPPLRLGSSEMYGFFVKGSRDKLQRTVD
                     ETLNAAAAGRMRFKVLSPYVLLTFTRVQHAQSTDPVDSAKGWGKETDIVTWVMVGQVL
                     PGQTRIHHIYVYPCHIFVDDAMALINGRELYGYPKYACEYTMPAAGEEPSAFTLAAKG
                     FQPFGTDTQLAMHPLLEVKASQARGPHTPLQDWDQLVKQLLELLASEVDALDLDAAGW
                     EQVEQALLKPQLDQIFLKQFPDGAGEKAVYQAIVTAPAVVKQVHSVELLGYSYDCTLH
                     AFDSFPLDRTLGLQLGAQPAILPFHLSFDFEVTQGEELVDNSQVQPQKVAILGGGVGA
                     MTAAYYLSDQPGWQNRYDITVYQMGWRIGGKGASGRNPAMGQRIEEHGLHIWFGFYDN
                     AFALMQEAYELLQRPPGAPLATWRDAFKPQHFVTLTEYINEQWKIWPIDTPIKPGEPG
                     RASERIDLWSTSLTLYAWIRQWLPEAHRELELAEPTPVVQACGGAHPDWLLRLAAAVG
                     HEQERLANDARRLGDALHDFALTLPADLSQHTEQHRELMRATLYCIRDWMAQTAARQL
                     DLSDRLRRLYICIDLACTALIGMLEDGVFTHGFDVINDIDFYAWLTEHGANQEYTVHS
                     APVRGFYDLVFAYEDGDFDRPNIEAGTMLHGMLRVAVAYHGGMMWKMQAGMGDTVFTP
                     LYQVLKARGVKFKFFHKVEELRPAADGSGVVDEIRLTRQADVTRGPDHYEPLVDVKGL
                     ACWPSSPNYGQILPAQAELLQANSINLESNWSNWPELYQQHFGQPLPELTLKRGRDFD
                     LVVYGISVASMTGLCPQLLAQSPPLERMAKRVKTVATQAYQVWLDKSTPELGWSSFAP
                     GDGQEPVLSGFTEPFDTWAPMDQLLR"
     gene            2903..>3227
                     /locus_tag="VFV25_08505"
     CDS             2903..>3227
                     /locus_tag="VFV25_08505"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY1393406.1"
                     /translation="MTKPSTLPTRDGLALHLRHWPTAPGASARGRILLVHGLGEHIGR
                     HERLAAELNAQGWAVAGYDLRGHGRSEGAKGRVRKTDDMLSDLAAVIDSLRSELPKLP
                     LLLLGH"
BASE COUNT          511 a         1066 c         1079 g          571 t
ORIGIN      
        1 cgcagcagct gatccatggg cgcccaggtg tcgaagggct cggtgaatcc gctcagcacg
       61 ggctcctggc cgtcgccggg cgcaaagctg gaccaaccga gctcgggggt gctcttgtcc
      121 agccagacct ggtaggcctg ggtcgcgacg gtcttgacgc gcttggccat gcgctcgagc
      181 ggcgggctct gcgccaggag ctgcgggcac aggccggtca tggaagccac ggagatgccg
      241 tacacgacga gatcgaagtc ccggccccgc ttgagcgtga gctcgggcag cggctgacca
      301 aaatgctgct ggtagagctc gggccagttg ctccagttgg attcgaggtt gatggagttg
      361 gcctgcagca gctcggcctg tgccggcagg atctgcccgt agttcgggct gctgggccag
      421 caggccaggc ccttcacgtc gaccaggggc tcgtagtggt ccgggccgcg cgtgacgtcc
      481 gcctgccgcg tcagccggat ctcgtcgacc acgccggagc cgtcggctgc cggcctcagc
      541 tcctctacct tgtggaagaa cttgaacttc actccgcggg ccttcagcac ctggtacagc
      601 ggggtgaaca ccgtgtcgcc catgccggcc tgcatcttcc acatcatgcc gccgtggtag
      661 gccacggcca cgcgcagcat gccgtgcagc atggtgccgg cctcgatatt gggcctgtcg
      721 aaatcgccgt cctcataggc gaacaccagg tcatagaagc cgcgcactgg cgccgagtga
      781 acggtgtact cctggttggc gccgtgctcg gtgagccagg cgtagaagtc gatgtcgttg
      841 atgacgtcga agccgtgcgt gaacacgccg tcctcgagca tgccgatcag cgcggtgcat
      901 gccaggtcga tgcaaatgta gagccgacgc agacggtcgc tcaggtcgag ctggcgtgcg
      961 gcggtttgcg ccatccaatc gcggatgcag tacagggtag cgcgcatcag ctcgcgatgc
     1021 tgctcggtgt gctggctcag atccgcgggc agcgtgagcg cgaagtcgtg cagggcgtcg
     1081 cccaggcgcc tggcgtcgtt ggccaggcgt tcctgctcgt gtcctacagc ggccgccagg
     1141 cgcagcagcc aatctgggtg agcgccgccg caggcctgca cgacgggtgt tggttcggca
     1201 agttccagtt cacgatgcgc ttcaggcaac cactgcctga tccacgcgta cagcgtgagg
     1261 ctcgtgctcc acaggtcgat gcgctcgctg gcacgcccgg gttcgccggg tttgatcggc
     1321 gtatcgatgg gccagatctt ccactgctcg ttgatgtatt cggtgagcgt gacgaaatgc
     1381 tggggcttga aggcatcgcg ccaggtcgcc agcggcgcgc caggcgggcg ctgcagcagc
     1441 tcataggcct cctgcatcag cgcgaaggcg ttgtcataga agccgaacca gatatgcaga
     1501 ccgtgctctt cgatgcgctg gcccatggcc gggttgcggc cgctggcgcc cttgccgccg
     1561 atgcgccagc ccatctggta gaccgtgatg tcgtagcggt tctgccagcc gggctgatcg
     1621 ctcaggtaat aggccgccgt catcgcgccg acgccgccgc cgaggatggc caccttctgc
     1681 ggctgcactt gcgagttgtc caccagttcc tcgccctggg tcacttcgaa gtcgaagctc
     1741 agatggaagg gcaggatggc aggctgcgcg ccgagctgca gccccaacgt gcggtccagc
     1801 ggaaagctgt cgaaggcatg cagggtgcag tcatagctgt agcccagcag ctccacgctg
     1861 tgcacctgct tcacgacagc cggcgcggtc acgatggcct gatagaccgc cttctcgccc
     1921 gcgccatcgg gaaactgctt caggaagatc tggtccaact gcggcttcag caaggcctgc
     1981 tccacctgct cccacccggc cgcgtcgagg tccaaggcat cgacctcgct ggccagcaac
     2041 tcgaggagct gcttcaccag ctggtcccag tcctgcagcg gcgtgtgcgg cccgcgggcc
     2101 tgcgaggcct tcacctcgag cagcggatgc atcgccagct gcgtgtccgt gccgaagggc
     2161 tggaagccct tggccgcgag cgtgaaggca ctgggctcct cgcctgccgc cggcatcgtg
     2221 tattcgcagg cgtacttggg gtagccgtag agctcgcgcc cgttgatgag cgccatcgca
     2281 tcgtccacga agatgtggca gggatagacg tagatgtggt ggatgcgcgt ctgccccggc
     2341 agcacctggc cgaccatgac ccaggtgacg atgtcggtct ccttgcccca gcctttggcg
     2401 ctgtccacgg gatcggtcga ttgcgcgtgc tgcacccgcg tgaaggtgag cagcacatag
     2461 ggcgaaagca ccttgaagcg catgcgcccg gcggccgccg cattgagcgt ttcgtccacc
     2521 gtgcgctgca gcttgtctcg gctccccttg acgaagaagc cgtacatctc ggaactgccg
     2581 agccgcagag gcgggtgcat caccacgttg ccgccgctgt agatgtagtc gggtcgctgc
     2641 gccatgcgcc agtcctttct tttggttcgt tgttggttgc agcggcatgg ccggggtcga
     2701 ccgcgggccg cggtgaacag cggccgctat ttgcaatcgc ttacaagccg ggcgactgtg
     2761 tctgccgcat gtgacgaagt caagaacgac agcggcgatc aatgacaagt agttcacgca
     2821 agcccgctct ggcgcgcgcg tcgcccctgt tgacccggcc ggcgctggct cgcacccctg
     2881 atccccaata gactgcgcac ccatgaccaa gccctccacc ctgcccacgc gcgatggcct
     2941 cgcgctccac ttgcgccatt ggcccaccgc gcccggcgcc tctgctcgcg gccgcatcct
     3001 gctcgtgcac gggctgggcg aacacatcgg gcgccacgaa cgcctcgcgg ccgaactcaa
     3061 tgcccagggc tgggcggttg cgggctacga cttgcgcggg cacggccgct ccgagggcgc
     3121 gaagggcagg gtgcgcaaga ccgacgacat gctgagcgac cttgccgcgg tcatcgacag
     3181 cttgcgctcc gagctgccca agctcccgct gctgctgctg ggccaca
//
LOCUS       DASUCY010000426         2698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_170287, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000426 DASUCY010000000
VERSION     DASUCY010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170287"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..377
                     /gene="yajC"
                     /locus_tag="VFV25_08510"
     CDS             54..377
                     /gene="yajC"
                     /locus_tag="VFV25_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEY1393407.1"
                     /translation="MFISNAYAQTAGGAGGGTDGLLGMLPLVLMFVVLYFVMIRPQMK
                     RQKEQKTMVEALAKGDEVVTSGGMLGKITKLGETYLSIEVANGVELQVQRGAVVQVLP
                     KGTIK"
     gene            477..2342
                     /gene="secD"
                     /locus_tag="VFV25_08515"
     CDS             477..2342
                     /gene="secD"
                     /locus_tag="VFV25_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEY1393408.1"
                     /translation="MNRYPWWKYAIIVVALLVGTIYTLPNFFGEAPAVQVSSGKVTLK
                     LDTSYVARVQDALTQAGVAPDYVQFDGNSVKARFTDTDTQIKARDALAKALNPDPSDP
                     GYIVALNLLSRSPTWLATLHALPMYLGLDLRGGVHFLMQVDMKAAITKKADAIVGDVR
                     LGLRDKNIRNSGTARVGDGVEVSFRDRDTLAAALPVLSDQQPDMNWVESADASGGYKL
                     SGSFKPEALRRVQDQAIKQNITTLHNRVNELGVAEPVIQQQGADRVVVQLPGVQDTAK
                     AKDIIGRTATLELRMVDDSAEALAAVTGSGPVPFGDERYVERGGAPLIVKRQVVITGE
                     NLSDAQAGFDGQTHEPAVHLTVDAKGARIMRDISRDNIGKRMAILLFEKGKGEVITAP
                     VIRGELGNRFQISGRMTTTEAADTALLLRAGSLAAPMEIIEERTIGPTLGAENIAKGF
                     DSVLYGFAAIAAFMCLYYLLFGLFSTLALAVNLLFLIAILSMLQATLTLPGIAAIALV
                     LGMAIDSNVLINERVREELRGGASPQMAIHSGYERAWATILDSNVTTLIAGLALLAFG
                     SGPVRGFAVVHCLGILTSMFSAVFFSRGVVNLWYGRQKKLKSVLIGQVWKPAGEQ"
     gene            2376..>2698
                     /locus_tag="VFV25_08520"
     CDS             2376..>2698
                     /locus_tag="VFV25_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEY1393409.1"
                     /translation="MEFFRIKRDIPFMRYALVFNVISFLTFAAAVFFLITRGLHLSIE
                     FTGGTVIEVAYAKSADIGKTRAAVEALNLGDVQVQNFGTSHDVLIRLPLHGDSKQQAA
                     VVDRV"
BASE COUNT          463 a          913 c          882 g          440 t
ORIGIN      
        1 gcgccgcata caatctactg tttgtttccc gcaacgattt gaaggacagc gacgtgttca
       61 tttccaacgc ctacgcgcag acggccggtg gcgccggcgg cggcaccgac ggcctgctgg
      121 gcatgctgcc cctggtgctg atgttcgtgg tgctctactt cgtgatgatt cgtccgcaga
      181 tgaagcgcca gaaggagcag aagaccatgg tcgaagcact cgccaagggc gacgaggtcg
      241 tcaccagcgg cggcatgctc ggcaagatca ccaagctcgg tgaaacctat ctgagcatcg
      301 aagtggccaa cggcgtggag ctgcaggtgc agcgcggtgc cgtggtgcaa gtgctgccta
      361 agggcaccat caagtaagta atcggcgcgg cgctttcccg caagccatac cccaaaaggc
      421 cgcggccgtc atgagctgac gccgcggcct gcctttcggt tcacccgagg acatccatga
      481 accgctaccc ctggtggaag tacgcgatca tcgtcgtcgc gctgctcgtc ggcaccatct
      541 ataccctgcc caatttcttc ggcgaggcgc cggcggtgca ggtgtccagc ggcaaggtca
      601 cgctcaagct cgacacgagc tatgtggccc gcgtgcagga cgctctcacg caggccggcg
      661 tcgcgcccga ctatgtgcag ttcgacggca attcggtcaa ggcgcgcttc accgacaccg
      721 acacccagat caaggcccgc gacgcgctcg ccaaggcgct caatcccgat cccagcgacc
      781 cgggctacat cgtcgcgctg aacctgctgt cgcgctcgcc cacctggctc gccacgctgc
      841 atgccctgcc catgtatctg ggtctggacc tgcgcggcgg tgtgcatttc ctgatgcagg
      901 tggacatgaa ggcggccatc accaagaagg ccgatgccat cgtcggcgac gtccgcctgg
      961 gcctgcgcga caagaacatc cgcaacagcg gcacggcgcg cgtgggcgac ggcgtcgagg
     1021 tgagcttccg cgaccgtgac acattggccg cggcgctgcc cgtgctgagc gaccagcagc
     1081 ccgacatgaa ctgggtcgag agcgccgacg ccagcggcgg ctacaagctc agcggcagct
     1141 tcaagcccga ggcgctgcgc cgcgtgcagg atcaggccat caagcagaac atcacgacgc
     1201 tgcacaaccg cgtcaacgag ctcggcgtgg ccgagccggt gatccagcag cagggcgccg
     1261 accgcgtcgt ggtgcagctg ccgggcgtgc aggacacggc caaggccaag gacatcatcg
     1321 gccgcaccgc gacgctggag ctgcgcatgg tggacgacag cgccgaggcg ctggccgccg
     1381 tcaccggtag cggcccggtg cccttcggcg acgagcgcta tgtggagcgc ggcggcgcgc
     1441 cgctgatcgt caagcgccag gtcgtcatca ccggcgagaa cctgtccgac gcccaggccg
     1501 gcttcgacgg ccagacccac gagccggccg tgcacctgac cgtggacgcc aagggcgcgc
     1561 gcatcatgcg cgacatctcg cgcgacaaca tcggcaagcg catggccatc ctgctgttcg
     1621 agaagggcaa gggcgaggtc atcacggccc cggtgatccg cggcgagctg ggcaaccgct
     1681 tccagatctc gggccgcatg acgacgaccg aggcggccga caccgcgctg ctgctgcgcg
     1741 caggcagcct ggccgcgccg atggagatca tcgaggagcg caccatcggc ccgacgctgg
     1801 gcgccgagaa catcgccaag ggcttcgaca gcgtgctcta cggcttcgcc gcgatcgccg
     1861 ccttcatgtg cctgtactac ctgctgttcg gcctgttctc gacgctggcg ctggccgtga
     1921 acctgctgtt cctgatcgcg attttgtcga tgctgcaggc cacgctgacg ctgccgggca
     1981 tcgccgccat cgcactggtg ctgggcatgg cgatcgactc caacgtgctg atcaacgagc
     2041 gcgtgcgcga ggaactgcgc ggcggcgcct cgccgcagat ggcgatccac tccggctacg
     2101 agcgcgcctg ggccaccatc ctggactcca acgtcaccac gctgatcgct ggcctggcgc
     2161 tgctggcctt cggctccggt ccggtgcgcg gcttcgcggt ggtgcactgc ctgggcatcc
     2221 tgacctcgat gttctcggcc gtgttcttct cgcgcggcgt cgtgaacctc tggtacggcc
     2281 gccagaagaa gctcaagtcg gtgctgatcg gccaggtctg gaagccggcc ggcgagcagt
     2341 aaagcaggtc cagcggccca ttacgagagc aagccatgga attcttccgt atcaagcgcg
     2401 acatcccgtt catgcggtat gcgctggtgt tcaacgtcat ctccttcctg acctttgcgg
     2461 cggccgtctt cttcctgatc acgcgcgggc tgcatctgtc gatcgagttc accggcggca
     2521 cggtgatcga ggtggcctat gcgaaatcgg cggacatcgg caagacgcgc gcggcggtcg
     2581 aggcgctgaa cctgggcgac gtgcaggtgc agaacttcgg cacctcgcac gacgtgctga
     2641 tccgcctgcc gctgcacggc gacagcaagc aacaggccgc ggtcgtcgat cgcgtctt
//
LOCUS       DASUCY010000427         3348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1294842, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000427 DASUCY010000000
VERSION     DASUCY010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294842"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1153
                     /locus_tag="VFV25_08525"
     CDS             <1..1153
                     /locus_tag="VFV25_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SelB C-terminal domain-containing protein"
                     /protein_id="HEY1393410.1"
                     /translation="WLCTPEVALSSDRIDARLSLWHGEARSLRSGASVHVHLGASDVM
                     GSVAMLDEGDALAPGAAARVQLVLREPIGVWRGERIVLRDASASRTLAGGVVLDPEAP
                     QRYRRTAQRLAALAAWEAEEPAERLRALLHASPGGVDLEAWLRAEGRVATALPALPVG
                     VLRGGDANADWALAAAHAEALRTAVLDALAAFHRQQPDELGPDTARLRRLAAPRLAEP
                     LWRVLLTALQREGAVMQHGAQLHLPEHGVQLSAQEQRIAQKVLPRLEAGGFDPPWVRD
                     LAADTRESEALLRAVLARLARRGELHQVVKDLYYPAGTMKRLAQLARERAGPDGELTA
                     ASFRDATQLGRKRAIQILEFFDRVGLLRRVHDAHRLRSDCSLFVEQEPR"
     gene            1164..1256
                     /locus_tag="VFV25_08530"
     tRNA            1164..1256
                     /locus_tag="VFV25_08530"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1198..1200,aa:Sec,seq:tca)
     gene            1407..1847
                     /locus_tag="VFV25_08535"
     CDS             1407..1847
                     /locus_tag="VFV25_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEY1393411.1"
                     /translation="MNIAGFEFPEELHYLVPHDTWARLDADGLVRVGITSLGVHMAGE
                     LYMCRPQPMGREVEAGRSIAVVELAKSIVSVKSPLSGTVAEVNAALEDHPELVYRDCY
                     RGGWLARLRPADWARDRQGLVTGGAAIAAAMRRQLWLHGLTPEP"
     gene            1849..2262
                     /locus_tag="VFV25_08540"
     CDS             1849..2262
                     /locus_tag="VFV25_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEY1393412.1"
                     /translation="MTMTLAKTDIAPPPRSVALLLWSCEPEAPHRLATPFFHASAAAA
                     LDIPVEIYFTARSVLLLKPGVAAQLYASEAGDKSIYDYMQEAAGLGARFLACSDALAA
                     HGLLDQPLIPECKGRGGAVQFMARACDPNWTALVF"
     gene            complement(2289..2822)
                     /locus_tag="VFV25_08545"
     CDS             complement(2289..2822)
                     /locus_tag="VFV25_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mpo1-like protein"
                     /protein_id="HEY1393413.1"
                     /translation="MRTATDLLSQYAAYHRDRRNITTHFVGIPMIVFAVGVLLARPGF
                     TVAGLGLTPAWIAFVPAALWYLTRGNLALGLATSAATGLLLLLAHRVAGGSTGAWLAW
                     GLGCFFVGWAIQFLGHYYEGRKPAFVDDLAGLLVGPMFVTAEALFAAGWNKPLLAEIE
                     RRAGPTMLRDLATPQHG"
     gene            2992..>3348
                     /gene="dcd"
                     /locus_tag="VFV25_08550"
     CDS             2992..>3348
                     /gene="dcd"
                     /locus_tag="VFV25_08550"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Catalyzes the formation of dUTP from dCTP in
                     thymidylate biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEY1393414.1"
                     /translation="MSIKSDKWIRRMAEQHGMIEPFEPNQVRYAADGHKIVSYGTSSY
                     GYDIRCAPEFKVFTNIYSTVVDPKAFDEKSFVDIESDCCIIPPNSFALARTMEYFRIP
                     RNVLTICLGKSTYARCG"
BASE COUNT          500 a         1111 c         1209 g          528 t
ORIGIN      
        1 gtggctgtgc acgcccgagg tcgcgctgag cagcgatcgc atcgacgcgc ggctgagcct
       61 gtggcatggc gaggccagga gcctgcgttc cggcgccagc gtgcatgtgc acctcggtgc
      121 gagcgatgtg atgggttccg tggcgatgct cgacgaaggc gatgcgctgg cgccgggcgc
      181 cgcggcccgc gtgcagttgg tgctgcgcga gccgataggc gtctggcgcg gcgagcgcat
      241 cgtgctgcgc gatgcctcgg cgagccggac gctggccggc ggcgtcgtgc tggaccccga
      301 ggcgccgcag cgctaccgcc gcacggcgca gcgcctggcg gcgctcgcgg cctgggaagc
      361 cgaggagccg gccgagcggc tgcgcgcctt gctgcacgcc tcgccgggcg gcgtcgacct
      421 cgaggcctgg ttgcgcgccg agggccgcgt tgcaacggcc ttgccggctt tgccggtcgg
      481 tgttctgcgg ggcggcgacg ccaatgcgga ctgggccttg gcagccgcgc atgccgaagc
      541 gctcaggacc gctgtgctcg acgcgctggc ggcttttcat cggcagcagc ccgatgagct
      601 ggggccggat acggcccgac tgaggcgctt ggccgcgccg cggctggccg agccgctgtg
      661 gcgcgtgctg ctcaccgcgc tgcagcgcga gggcgcggtg atgcagcatg gtgcccagct
      721 tcatctgccc gagcatgggg tgcagctctc ggcgcaggag cagcgcatcg cgcagaaggt
      781 gctgccacgc ctggaggcgg gcggcttcga cccgccctgg gtgcgcgacc tggccgccga
      841 tacccgcgag agcgaggcgc tgctgcgcgc cgtgctggca aggctggcgc ggcgcggcga
      901 gctgcaccag gtcgtgaagg acctgtacta tcccgccggc acgatgaagc ggctggcgca
      961 actggcacgc gagcgggccg gcccggacgg cgagctgacc gcggcgagtt ttcgcgacgc
     1021 cacgcagctg ggccgcaagc gggcgatcca gatcctggaa ttcttcgacc gcgtggggtt
     1081 gctgcgtcgc gtgcacgatg cgcacaggct gcgcagcgac tgctcgctgt tcgtcgagca
     1141 ggaaccgcgg tgagaaagat catggagggg aaacgatcct ggtgggtcgg ccgggcttca
     1201 aacccggtgg gtggcgccac gcgccgctgg gagggttcga ctccctctcc cctccgccga
     1261 aagcattcgc ttggctgctg cgccgggcga tctcgcgatt gcattgctcg ccgtacggga
     1321 gtactgcgct acccgtcgcg atctcgaccc gctcgcgacg ccaatcgaac gctttcggct
     1381 cgcttcggct tcgccattgg ctcgccatga acattgctgg ttttgagttc cccgaagagc
     1441 tccactatct ggtgccgcac gatacatggg cgcggctgga cgccgatggg ctggtccgcg
     1501 tgggcatcac gtccttgggg gtccatatgg cgggcgagct ttacatgtgc cggccgcagc
     1561 cgatggggcg ggaggtcgag gcggggcgct cgatcgccgt ggtggagctc gcgaagtcca
     1621 tcgtctcggt gaagagtccg ctatcgggta ccgtcgcgga agtgaacgcg gcgctcgaag
     1681 accatcccga gctggtgtat cgcgattgct accgcggcgg ctggctggcg cgcctgcggc
     1741 ctgcggattg ggcgcgcgac aggcagggct tggtgaccgg cggtgccgcg atcgcagccg
     1801 ccatgcggcg gcagttgtgg ctgcatggcc tgacgccgga accctgacat gacgatgacc
     1861 ctggcgaaga ccgacatcgc accgccgccg cgcagcgtgg cgttgctgct gtggtcctgc
     1921 gagccggagg cgccgcaccg cctcgccacg ccctttttcc acgccagcgc cgccgccgcg
     1981 ctggacatcc cggtggagat ctatttcacg gcgcgatcgg tgctgctgct caagcccggc
     2041 gtggcggccc agctctacgc aagcgaggcg ggcgacaagt cgatctacga ctacatgcag
     2101 gaggccgccg ggctgggcgc gcgcttcctg gcctgcagcg acgcgctcgc ggcgcatggc
     2161 ttgctggacc agccgctgat cccggaatgc aagggccgcg ggggcgcggt gcaattcatg
     2221 gcccgcgcct gcgatccgaa ctggactgcg ctggtgttct agtggtcgcc agtgtgcacc
     2281 cgcgaaccct agccgtgctg gggcgttgcc aggtcgcgca gcatcgtcgg cccggcgcgc
     2341 cgctcgatct cggccagcag cggcttgttc cagccggccg cgaacaaggc ctcggccgtc
     2401 acgaacatcg ggcccaccag caggccggcc aggtcgtcga cgaaggccgg cttgcggccc
     2461 tcgtagtaat ggccgaggaa ctggatggcc cagccgacga agaagcagcc caggccccag
     2521 gccagccagg cgccggtgct gccgcccgcg acccggtggg ccagcagcag cagcaagccg
     2581 gtggcggcgc tggtggccag gcccagtgcc aggttgccgc gcgtgagata ccacagcgcg
     2641 gccggcacga aggcgatcca ggccggtgtc agcccgagcc cggccaccgt gaagcccggc
     2701 cgcgcgagca gcacgccgac ggcgaagacg atcatgggaa taccgacgaa atgcgtcgtg
     2761 atattgcgcc ggtcgcggtg ataggccgcg tattggctga gcaggtcggt ggcggttctc
     2821 atgcgtagct cccggaacac atcctcatcg cgtcaatgct agtgtgacgc ggccagtttc
     2881 gctgtcaacg attcgtcacc tttgctaacc ggagttgtct cgggatcaag acgcgcgtgc
     2941 cgggagatct gcggcgtctc cctacaatcc gggctagtta cgggatcagc gatgagcatc
     3001 aagtccgaca agtggatacg ccgcatggcc gagcagcacg gcatgatcga gcccttcgag
     3061 cccaaccagg tgcgctatgc ggccgacggc cacaagatcg tgagctacgg caccagcagc
     3121 tatggctacg acatccgctg cgcgccggag ttcaaggtct tcaccaatat ctacagcacg
     3181 gtggtcgacc ccaaggcctt cgacgagaag agcttcgtcg acatcgagtc cgactgctgc
     3241 atcatcccgc ccaacagctt cgcgctggcg cgcacgatgg agtacttccg cattccgcgc
     3301 aatgtgctga ccatctgcct gggcaagagc acctatgcgc gttgcggc
//
LOCUS       DASUCY010000428         3032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1294859, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000428 DASUCY010000000
VERSION     DASUCY010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294859"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..766
                     /locus_tag="VFV25_08555"
     CDS             17..766
                     /locus_tag="VFV25_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEY1393415.1"
                     /translation="MLLKDKIALVTGGAGVNGLGYATARMMAAQGARVVILDLERANP
                     AGAAAQLGPQHVGLVADVTDKASCERATKAALERCGRIDILVNNAGITQPRKTADISA
                     ADYDAVLDVSLRGTLHMSQAVLPAMRAAKSGAIVCISSVSAQRGGGILGGPHYSAAKA
                     GVLGLARAMAREFGIDGIRVNCVTPGLIGTDINKGLIPDDRMKGILEQIPLNRIGEPA
                     DVAGCVVFLASPLAKYCTGVTLDVNGGMLIH"
     gene            830..1861
                     /locus_tag="VFV25_08560"
     CDS             830..1861
                     /locus_tag="VFV25_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY1393416.1"
                     /translation="MTTPETPRSGMTRRQCLIGAAALPLFTILPRRARAAEFTYKLAT
                     GQDPSHPVNKRAQEAIDRIRAATNGRLEIRLFPANQLGSDTDLLSQVRNGGVEFFNQA
                     SSVLSTLVPVAGIVNTGFAFNDSEQVWKAMDGGLGKYVRGQIEKVGLLTMSKPWENGF
                     RQMTTSTKAIHSPDDLRGLKLRVPSAPMLSTLFTALGASPTPINFNEVYSALQTKLVE
                     GQENPLAIIATARLYEVQKFCSLTNHVWDAYWILGNRKAVERLPKNIQEIVFREFEKS
                     ANDERADIAALSKSLRADLQAKGLQFIDVDKKAFKAALGKTSFYKDWRAKFGDEAWKL
                     LEDSAGTLA"
     gene            1934..>3032
                     /locus_tag="VFV25_08565"
     CDS             1934..>3032
                     /locus_tag="VFV25_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HEY1393417.1"
                     /translation="MSRPIPSNLANAIRFLAIDAIVRAGEGHQGVPLGMAEIATALYM
                     RHLKFLSTDPTWPDRDRVVLSNGHGSMLLYALLHLTGYEAISIDEIKRFRELGSRCEG
                     HPEINPAHGIEVTTGPLGQGIANAFGMAVAEAYLNARCGSGLVDHYTYAFVGDGCLQE
                     GVGQEMISLAGHLRLGKLVLLWDDNQITDDGSTALSISENVAERLRVADWHVVEVDGH
                     DVEAVSAALAAARLDPRPSFIACRTVIARGIARLQGQRGGHSARLHPEDADVARQQLD
                     WPHPPFEVPAPVLRAWREAGQRCASEHAAWQARRDALPPPERAEFERVLAGELPAGWR
                     EVLLDVKRRALERAEAQGGIMISAEINDRLSE"
BASE COUNT          520 a         1094 c          980 g          438 t
ORIGIN      
        1 tcactggagt acagacatgc ttctcaagga caaaatcgct ctcgtcaccg gcggtgccgg
       61 cgtcaacgga ctcgggtacg ccactgcgcg catgatggcc gcgcaaggcg cgcgcgtcgt
      121 gatcctcgac ctcgaacgcg ccaaccccgc cggcgccgcc gcccagctcg ggccgcaaca
      181 cgtcggcctg gtcgccgacg tgaccgacaa ggcgtcctgc gagcgcgcca ccaaggcagc
      241 gctcgagcgg tgcggccgca tcgacatcct ggtcaacaac gccggcatca cgcaaccgcg
      301 caagaccgcc gacatcagcg ctgccgacta cgacgccgtg ctcgacgtca gcctgcgcgg
      361 caccctgcac atgtcacagg ccgtgctgcc ggcgatgcgc gcggccaagt ccggcgccat
      421 cgtctgcatc tcgtcggtct cggcgcagcg cggcggcggc atcctcggcg ggccgcacta
      481 ctcggccgcg aaggccggtg tgctcggact ggcgcgggcg atggcgcgcg agttcggcat
      541 cgacggcatc cgcgtcaact gcgtcacccc gggcctgatc ggcaccgaca tcaacaaggg
      601 cctgatcccc gacgaccgca tgaagggcat cctcgagcag attcctctaa accgcatcgg
      661 cgagcctgcc gacgtcgccg gctgcgtcgt gttcctggcc agcccgctgg ccaagtactg
      721 caccggcgtc acgctcgacg tgaacggcgg catgctgatt cactgagcgc acgcacccgc
      781 cggcgccgca gcggcccagc caactacaag cgatacaagg agacaagcga tgaccacgcc
      841 cgagacccct cgttccggca tgacccgccg gcaatgcctg atcggcgccg cggcgctgcc
      901 cctgttcacc atcctgccgc ggcgtgcccg cgccgccgag ttcacctaca agctcgccac
      961 cgggcaggac ccgagccacc ccgtcaacaa gcgcgcgcag gaggccatcg accgcatcag
     1021 ggcggccacc aacggccggc tcgagatccg cctgttcccc gccaaccagc tcggctcgga
     1081 caccgacctg ctgtcccagg tgcgcaacgg cggcgtggag ttcttcaatc aggccagttc
     1141 ggtgctgtcg accctggtgc cggtggccgg catcgtcaac accggcttcg ccttcaacga
     1201 cagcgagcag gtctggaaag ccatggacgg cggcctgggc aagtatgtgc gaggccagat
     1261 cgagaaggtc ggcctgctga cgatgtccaa gccgtgggaa aacggcttcc ggcagatgac
     1321 cacgtcgaca aaagccatcc actcgcccga cgacctgcgt ggcctgaagc tgcgcgtgcc
     1381 ctcggcgccg atgctctcga cgctgttcac cgcgctaggc gccagcccga cgccgatcaa
     1441 cttcaatgag gtctattccg cgctgcagac caagctcgtc gagggccagg agaacccctt
     1501 ggccatcatc gcgacggcgc ggctgtacga ggtgcagaag ttctgcagcc tgacgaacca
     1561 tgtgtgggac gcctattgga ttctcggcaa ccgcaaggcc gtcgagcggc tgccgaaaaa
     1621 catccaggaa atcgtgttcc gcgagttcga aaagtcggcc aacgacgagc gtgccgacat
     1681 cgcggccctg agcaagtcct tgcgcgccga tctccaagcc aagggcctgc agttcatcga
     1741 cgtcgacaag aaggccttca aggccgcgct cggcaagacc agtttctaca aggactggcg
     1801 cgccaagttc ggcgacgagg cttggaagct gctggaagac agcgccggca ccctggcctg
     1861 aggccgccgc atccgccgcg attgcgcaac ccgccgcccc cgtgaaccga gggcggcaca
     1921 gcagagcatt gccatgtctc gaccgatccc atcgaacctc gccaacgcca ttcgcttcct
     1981 tgccatcgac gccatcgtgc gcgccggcga gggtcatcag ggggtgccgc tgggcatggc
     2041 ggagatcgcc acggcgctat acatgcgcca cctgaaattc ctgtccaccg acccgacctg
     2101 gcccgaccgc gaccgcgtcg tgctctccaa cggccacggc tcgatgctgc tgtatgcgct
     2161 gctgcatcta accggctatg aggccatctc gatcgacgag atcaagcgct ttcgcgaact
     2221 cggttcgcgc tgcgaagggc acccggagat caacccggcc cacggcatcg aggtgacgac
     2281 cgggccgctc ggccagggca tcgccaatgc gttcggcatg gcggtggccg aggcctatct
     2341 caacgcgcgc tgcggcagtg gcctggtgga ccactacacc tacgccttcg tcggcgacgg
     2401 ctgcctgcag gagggcgtgg gccaggagat gatctcgctg gccggccacc tgcgcctggg
     2461 caagctagtg ctgctgtggg acgacaatca gatcaccgac gatggcagca ccgcgctgtc
     2521 gatcagcgag aacgtcgccg agcgcctgcg cgtagccgac tggcacgtcg tcgaggtcga
     2581 cggccacgac gtcgaggcgg tttcggcagc gctggctgcc gcccggctcg acccccggcc
     2641 ttcgttcatc gcctgccgca ctgtcatcgc gcgcggcatc gcccggctgc agggccagcg
     2701 cggcggccac agcgcgcgcc tgcatccgga ggacgccgac gtcgcacgcc agcagctgga
     2761 ctggccgcac cctccgtttg aggtgcctgc gccagtgctg cgtgcctggc gcgaggccgg
     2821 ccagcgttgc gcttccgagc atgcggcctg gcaggcgcgg cgcgacgcgc tgcctccccc
     2881 ggagcgcgcg gagttcgagc gcgtcctcgc cggcgagctg cccgcgggct ggcgtgaggt
     2941 gctgctcgac gtcaagcgcc gcgcgctgga gcgtgccgag gcgcagggcg gcatcatgat
     3001 ctcagccgag atcaacgacc gtctgagcga gg
//
LOCUS       DASUCY010000429         3367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_812674, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000429 DASUCY010000000
VERSION     DASUCY010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812674"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..1014)
                     /locus_tag="VFV25_08570"
     CDS             complement(19..1014)
                     /locus_tag="VFV25_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEY1393418.1"
                     /translation="MPSHLPSSFDAARAVALAQETLSIEAQALTDLRSRVGEAFARAV
                     RLLLDCRGRVVVMGMGKSGHVGRKIAATLASTGTPSLFVHPAEASHGDLGMVTPGDMV
                     LAISNSGESDELTAILPVLKRLGVKILALTGRGESTLARHADIVLDSAVAKEACPLNL
                     APTASTTAQLALGDALAVALLDARGFREEDFARSHPGGALGRKLLTHVRDLMRTGDAL
                     PRVAPNTGFTAMMREMSSKGLGAAAITDEAGVVQGIFTDGDLRRLIEQGRDMRGLTAG
                     DVMHRQPRTVRPDALAVEAADLMEEARITLVLVVDAQGKLMGALNFNDLMRAKVI"
     gene            1093..3093
                     /locus_tag="VFV25_08575"
     CDS             1093..3093
                     /locus_tag="VFV25_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEY1393419.1"
                     /translation="MATNSLELALLFLLAAVVGVVACRSLKLPPMLGYLAAGILIGPN
                     ALGVTQDPAGVERLAQLGVVFLMFVIGLEFNLPKLRSMRTLVFGLGLSQVGLTMVLAL
                     GINASLAWGYAQLGRGWGLDWRSALALGAALAMSSTAIVVKLMAERLELESEHGRRVM
                     GVLLFQDLAVVPLLVLIPALGAAGHGMALALAVAAIKAAALLVLLLMGGQRVMRWWLT
                     LVARRRSEELFILNLLLVTLGLAWLTQLAGLSLALGAFVAGMLIAETEYKHQVETDIR
                     PFHDVLLGLFFISLGMKLDWREVAQHWALVLAMLSLPVLFKFGLVAALARTFGAAPGV
                     ALRTGLYLAQAGEFGFVLLTLADQNGLIPGRLGSPVLAAMVLSMLATPFIVMNSNRIV
                     MKLYASDWMMQSVALTSIARKAIKAEAHVIICGYGRSGQNLARLLEREGIPYMALDLD
                     PDRVRQAAAAGQSVVFGDAARLRSLMAAGLARASAVVVSYHDTPSALKILQLVHSHAP
                     TVPVIVRTIDDTDLEKLQAAGATEVVPEAIEGSLMMASHVLALVGVPMRRVIRVVQDQ
                     RDARYGLLRGYFHGADDDTAGELQQARLQSLNLNAGAACLGQALGLQALPTLGVSVVS
                     IRRASGQVLRPHDELALAEGDTLVLAGQPEALALGEDRLLRG"
     gene            complement(3154..>3367)
                     /locus_tag="VFV25_08580"
     CDS             complement(3154..>3367)
                     /locus_tag="VFV25_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEY1393420.1"
                     /translation="ICGEPGLFADGQRTANVEAVTPCVVWALRLPRFDELCIRVPQIA
                     LEVARAAAGVMAVRLRANMARQVPVC"
BASE COUNT          465 a         1165 c         1205 g          532 t
ORIGIN      
        1 ccggccgcaa ctcgcgcatc agattacctt ggcgcgcatc aggtcgttga agttcagcgc
       61 gcccatcagc ttgccttgcg catcgaccac cagcaccagc gtgatgcgtg cctcttccat
      121 caggtcggcc gcctccaccg cgagcgcatc ggggcgcacg gtgcgcggtt gcctgtgcat
      181 cacatcgccg gccgtcaggc cgcgcatgtc gcggccctgc tcgatcaggc ggcgcaggtc
      241 gccatcggtg aagatgccct gcaccacgcc ggcctcgtcg gtgatcgcgg cggcgcccag
      301 gcccttgctg ctcatctcgc gcatcatcgc ggtgaagccg gtgttgggcg cgacgcgcgg
      361 caaggcgtcg ccggtgcgca tcaggtcgcg cacatgggtc agcagcttgc ggcccagggc
      421 tccgccggga tgggagcgtg cgaagtcctc ctcgcggaag ccgcgcgcat cgagcagggc
      481 aaccgcgagc gcatcgccca gcgcgagctg ggccgtagtg ctggcggtgg gtgcgagatt
      541 gagcgggcag gcctccttgg cgacggcact gtccagcacg atgtcggcgt gacgggccag
      601 cgtcgattcg ccgcggccgg tgagcgccag tatcttgacg cccaggcgct tgagcacggg
      661 caggatcgcg gtcagctcgt cgctctcgcc ggaattggag atcgccagca ccatgtcgcc
      721 cggcgtgacc atgccgaggt cgccatggct ggcctcggcc ggatgcacga acagcgaagg
      781 cgtgccggtg gaggccagcg tggcggcgat cttgcggccc acatggccgc tcttgcccat
      841 gcccatcacg acgacgcggc cgcggcagtc gagcagcagc cgcacggcgc gcgcgaaggc
      901 ctcgccgacg cgcgaacgca gatcggtcag cgcctgggcc tcgatgctca gggtctcctg
      961 cgccagggcg acggcgcgcg cagcatcgaa ggaagaggga aggtgggagg gcacggggcg
     1021 cgggggcttg ggtaggatcg ggcgattcta ggctcgcagc gcctcggtac aaggcttgca
     1081 tacgccccgc gcatggccac caattcgctc gaactcgcat tgctgtttct gctcgctgcg
     1141 gtggtcggcg tggtggcctg ccgcagcctc aagctgccgc ccatgctggg ctatctggcc
     1201 gccggcatcc tgatcgggcc gaacgcgctg ggcgtgaccc aggacccggc cggcgtcgag
     1261 cgcctggctc agctcggcgt ggtcttcctg atgttcgtga tcgggctgga attcaacctg
     1321 cccaagctgc gcagcatgcg caccctggtg ttcggcctgg gcctgagcca ggtcggcctc
     1381 actatggtgc tcgcgctggg catcaacgcc tcgctcgcct ggggctatgc gcaactgggc
     1441 cgcggctggg gcctggactg gcgcagcgcg ctcgcgctcg gcgccgccct ggcgatgagc
     1501 agcaccgcta tcgtcgtcaa gctgatggcc gagcgcctgg aattggagag cgagcatggc
     1561 cgccgcgtga tgggcgtgct gctgttccag gacctggcgg tggtgccgct cctggtgctg
     1621 atcccggcgc tgggcgcggc cggccacggc atggcgctcg cgctggcggt ggcggcgatc
     1681 aaggccgctg cgctgctggt gctgctgctg atgggcggcc agcgcgtgat gcgctggtgg
     1741 ctcacgctcg tggccaggcg gcgcagcgag gagctgttca tcctcaacct gctgctggtg
     1801 acgctgggcc tggcctggct cacgcagctg gccggcttgt cgctggcgct gggcgccttt
     1861 gttgccggca tgctgatcgc cgagaccgaa tacaagcacc aggtggagac cgacatccgg
     1921 cccttccacg acgtgctgct gggcctcttc ttcatcagcc tgggcatgaa gctcgattgg
     1981 cgcgaggtgg cccagcattg ggccctggtg ctggccatgc tgagcctgcc ggtgctgttc
     2041 aagttcggcc tcgtcgccgc gctggcccgc accttcggcg ccgcgccggg cgtggcgttg
     2101 cgcaccggcc tgtacctggc ccaggccggc gagttcggct tcgtactgct gaccctggcc
     2161 gatcagaacg gcctcattcc cggccgcctg ggcagcccgg tgctggcggc gatggtgctg
     2221 tccatgctcg ccacaccctt catcgtgatg aacagcaacc gcatcgtgat gaagctctat
     2281 gccagcgact ggatgatgca atccgtggcg ctgaccagca tcgccaggaa ggcgatcaag
     2341 gccgaggccc acgtgatcat ctgcggctac ggccgctcgg gccagaacct ggcgcggctg
     2401 ctggagcgcg aaggcatccc ctacatggcg ctggacctcg acccggaccg cgtgcgccag
     2461 gccgccgccg cgggccagag cgtggtgttc ggcgatgcgg cgcgcctgcg cagcctgatg
     2521 gccgctggcc tggcgcgcgc gagcgcggtg gtggtgagct atcacgacac gccgtcggcg
     2581 ctgaagatcc tgcagctcgt gcattcgcat gctccgaccg tgccggtgat cgtgcgcacc
     2641 atcgacgaca ccgacctgga gaaactgcag gccgccggcg ccaccgaggt ggtgcccgag
     2701 gcgatcgagg gctcgctgat gatggcctcg catgtgctgg ccctggtcgg cgtgccgatg
     2761 cgccgcgtga tccgcgtcgt gcaggaccag cgcgacgcac gctacggcct gctgcgcggc
     2821 tatttccacg gcgccgacga cgacaccgcg ggagagctgc aacaggcccg gctgcagagc
     2881 ctgaacctca atgccggcgc cgcctgcctg ggccaggcgc tgggcctgca ggctctgccc
     2941 acgcttgggg tcagcgtggt ctcgatacgc cgcgccagcg gccaagtgct gcgtccgcat
     3001 gacgagctcg ccctggccga gggcgacacg ctggtgctgg ccggccagcc cgaggcgctg
     3061 gcgctgggcg aagaccgcct gctcagaggc tgatgcccgc ggccgcgcag ggaaagggcc
     3121 gcagatgcgg ccctcgttgt cgcctcggcc gactcagcag accggcacct ggcgtgccat
     3181 gttcgcgcgc aggcgcaccg ccatcacgcc ggcagcggcg cgcgccactt ccaacgcgat
     3241 ctgcggcacg cggatacaga gctcatcgaa gcgcggcagc ctcagcgccc agaccacgca
     3301 gggcgtcacc gcctcgacat tggccgtgcg ctgcccgtcg gcgaacagcc caggctcgcc
     3361 gcagatc
//
LOCUS       DASUCY010000430         2043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_668322, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000430 DASUCY010000000
VERSION     DASUCY010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668322"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VFV25_08585"
     CDS             <1..600
                     /locus_tag="VFV25_08585"
                     /inference="COORDINATES: protein motif:HMM:NF016301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEY1393421.1"
                     /translation="ERGKTEGLTPRCRRCDAPLMRSAGPPPDLPLALALTSLAALAVA
                     HTNPILGIEVQGRQQQASLWQAAMTLYDDQAAAMALLVLLTTLIAPLTEAIAVVWVLL
                     PLRQGCAAPGAPWLLRALQRVRPWVMIEVFMLGVLVSTVKLAAIASIQPGIGLGAFAV
                     AMLAIAGVGQSFDPERAWQALAEARKADTSGTVRHRTTA"
     gene            646..>2043
                     /locus_tag="VFV25_08590"
     CDS             646..>2043
                     /locus_tag="VFV25_08590"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEY1393422.1"
                     /translation="MSHRRKSWRAQFGPFTLIPSQRLLERAGTPVRLGGRALDLLIAL
                     VESAGKTVSKQDLIALVWPNAVVDEGSLRFHMVAVRKALGDGMDGRRYIVNTANKGYT
                     FVAAMERSELDTSAHPAPGGQSKSLPALGSTMVGRDAELEALDNAIAQRRLVSVVGSG
                     GIGKTTVAIVAAQSAARSFEGNVHFVDLSAVSTAELACSVVASVVGLQNRMDDPAALA
                     AHLVDRKALIVLDCCEHVIDSVAGLAEVLRRGCPQIHILATSREPLRAEGEFVYRLQP
                     LECPPDGEGTSSESALSYPAVKLFAERAAASGSGFVLSDSDAPLASRLCRELDGIALA
                     IELAAGRIEALGLKAITSHFDANVKLMWHGRRTAVPRHQTLSATLDWSYGLLDEDEKR
                     LLRRLSVFAGTFSLDAAIAVCCFDIGRPLAIEALAGLVSKSLLNVDAGGSTLRYRLFD
                     TTKAYTVQKLESSGEG"
BASE COUNT          329 a          693 c          666 g          355 t
ORIGIN      
        1 gagcgcggca agaccgaagg tctcacgccg cgctgccgcc gttgcgacgc gccgctgatg
       61 cgctctgcag gaccgccgcc agacctgccg ctggcccttg cgctcacttc gctggccgcc
      121 ctggccgtgg cgcacacgaa ccccatcctc ggcatcgaag tgcaggggcg gcaacaacag
      181 gccagcctgt ggcaggcagc gatgacgctc tacgacgacc aggccgccgc gatggccttg
      241 ctggtgctgc tgaccacgct gatcgcgccg ctcaccgagg caattgccgt ggtgtgggtg
      301 ctgctgccct tgcgccaggg atgcgccgcc cccggcgcac cctggctgct cagggcgctg
      361 caacgggtcc gtccctgggt gatgatcgag gttttcatgc tgggcgtgct ggtttcgacc
      421 gtgaagctcg ccgccatcgc ctccatccag ccgggcatcg gcctcggtgc atttgcagtc
      481 gcgatgctgg ccatcgccgg cgtcggccaa agctttgacc ctgaacgcgc ctggcaagcc
      541 ctggccgaag cacgcaaggc cgacacctcg ggcacggtcc ggcaccgaac cacggcatga
      601 gcccttccgc ccccacaatg gcctgaccag ttcatggact cgcctgtgtc ccaccgtcgc
      661 aagtcgtggc gcgcccagtt cggcccgttc accctgattc cctcccagcg cttgctggaa
      721 cgtgcaggca ccccggttcg actgggagga cgggcgctgg atctgctgat cgccctggtc
      781 gaatccgcag gcaagaccgt cagcaagcag gacctgatag ctctggtgtg gcccaacgcc
      841 gtcgtcgacg agggcagcct tcgcttccac atggttgctg tgcgcaaggc gcttggtgat
      901 ggcatggatg gcaggcgtta catcgtcaac acagccaaca agggctatac cttcgtcgcc
      961 gctatggagc gcagcgagct cgataccagc gcccatccgg cacctggtgg ccagagcaag
     1021 agcctgcccg cgctcggatc gaccatggtg gggcgtgatg cagagcttga agcgctcgac
     1081 aatgccatag cccagcggcg cctggtgtcc gtcgtcggct cgggcggcat tggaaagacg
     1141 acggtcgcga tcgttgccgc gcagtccgcc gccaggagct tcgaaggcaa cgtccacttc
     1201 gtcgacctct cggcggtttc gacggccgag ctggcgtgtt ccgtagtggc aagcgtggtg
     1261 ggactgcaga acaggatgga cgacccggca gccctggccg cacacctggt cgatcgcaag
     1321 gcgctcatcg tgctcgactg ctgcgagcac gtcatcgaca gcgtcgctgg actggcggag
     1381 gtgctcaggc gcggctgccc gcagattcac atcctcgcaa ccagccgcga accgttgcgc
     1441 gccgaagggg agttcgtcta tcgcctgcag ccgctggagt gcccacccga tggagaagga
     1501 acaagctccg agtcggccct ctcctacccg gccgtgaagc tcttcgcgga gcgtgcagcc
     1561 gcatcaggct cgggcttcgt gctcagcgac agcgacgcgc ctttggcctc gcgtctttgc
     1621 cgggagttgg atggcatcgc gcttgcgatc gaactcgcgg ccggccgcat tgaggccctc
     1681 ggcctgaagg cgatcacgtc gcacttcgat gcgaacgtca agctgatgtg gcacggcagg
     1741 cgcaccgccg tgccgcgcca tcaaaccctc agcgcgacgc tcgattggag ctacgggctt
     1801 cttgacgagg acgagaagcg cctgttgcgc cgactgtccg tgttcgccgg gactttctcg
     1861 ctggatgcgg ccatcgcggt ctgctgcttt gacatcgggc ggccgctcgc catagaggcc
     1921 ttggccggcc tggtttccaa gtcgctgctc aacgtcgatg cgggcggctc gacgctgcgc
     1981 tacagactgt tcgacaccac gaaggcttat accgtccaga agctggaaag ctccggtgaa
     2041 gga
//
LOCUS       DASUCY010000431         2780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_491575, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000431 DASUCY010000000
VERSION     DASUCY010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491575"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..137
                     /gene="trpD"
                     /locus_tag="VFV25_08595"
     CDS             <1..137
                     /gene="trpD"
                     /locus_tag="VFV25_08595"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348877.1"
                     /note="Catalyzes the conversion of
                     N-(5-phospho-D-ribosyl)-anthranilate and diphosphate to
                     anthranilate and 5-phospho-alpha-D-ribose 1-diphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEY1393423.1"
                     /translation="ALYAANVAASIAEGVKLAREAVASGKALAKLSQFVAVTQKLKSA
                     "
     gene            141..944
                     /gene="trpC"
                     /locus_tag="VFV25_08600"
     CDS             141..944
                     /gene="trpC"
                     /locus_tag="VFV25_08600"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEY1393424.1"
                     /translation="MGDILNKIVAVKHEEIAAALARRSLASLRAEVESAERRADLRDF
                     AGALRGKIAAGRAAVIAEVKKASPSKGVLREAFVPAEIAASYAKHGAACLSVLTDQRF
                     FQGAAEYLQQARAACALPVLRKDFMVDPYQVYEARAMGADCILLIAACLADAQMAELE
                     ACAQALGMAVLVEVHDGAELERALHLKTPLLGINNRNLRSFEVTLETTLSLQREVPAD
                     RLLVTESGILAREDVRRMREAGVHAFLVGEAFMRAPDPGAALAALFGEP"
     gene            956..1705
                     /locus_tag="VFV25_08605"
     CDS             956..1705
                     /locus_tag="VFV25_08605"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEY1393425.1"
                     /translation="MTSDAGLFDDGEPAGNRLSEPLDACFDRVQGAWREVTQRFRDSV
                     PGAALLGFVDARVKAGAVVYPATVFRALELMPLAAVRVVILGQDPYHGAGQAQGLAFS
                     VPSHLKKLPPSLRNIYAEVAADLGGSPARDGDLSRWAKQGVLLLNTTLTVEDGQPHSH
                     AGRGWEVLSDALVAAVSQQAQPSVFMLWGAPAQRKRALIDEGRHLVLTANHPSPLSAR
                     RPPQPFIGCRHFSQANDWLLRYRAPEAAVAW"
     gene            complement(1712..2725)
                     /locus_tag="VFV25_08610"
     CDS             complement(1712..2725)
                     /locus_tag="VFV25_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEY1393426.1"
                     /translation="MNSYRILKAQALICALAAATCLSAAAGEAAWPGFVYSPYKDITI
                     GLDAGTHRISPLRAIDADTPAAAAPTTLTLAFARGECGEENWAGHDAQAFADTNVAEL
                     VRADIDYVVSTGGQGNVFNCGSDAGMERFIARYASSHLIGFDLDIESGQTSEQIAALV
                     RRVKAAQARHPKLRFSFTLPTFAASDGSGASLNALGQRVLQSIRRHGLKAYTINLMVM
                     DYGAASPSACVVAAGRCDMGRSAIQAAHNLHTRYGLPFSQIELTPMIGVNDVKENVFT
                     LDDARLVAKFVRENKLAGLHFWSLDRDAPCPGDATEVSDRCSSMLAQPRGAYAQTFME
                     ALR"
BASE COUNT          428 a          918 c          971 g          463 t
ORIGIN      
        1 gcgcgctcta cgcggccaat gtggcggcct cgattgccga aggcgtgaag ctggcgcgcg
       61 aggcggtggc ttcgggcaag gccttggcca agctgagcca gttcgtcgct gtgacccaga
      121 aattgaaatc tgcctgagcc atgggcgata tcctgaacaa gattgttgcg gtcaagcatg
      181 aggagatcgc tgcggcgctt gcgcggcgct cgctggcttc gctacgtgcc gaggtcgagt
      241 cggccgagcg gcgcgccgac ctgcgcgatt tcgcgggcgc cttgcgcggc aagatcgcgg
      301 cgggccgagc ggcggtcatc gccgaggtca agaaggccag ccccagcaag ggcgtgctgc
      361 gcgaggcctt cgtgccggct gagatcgccg ccagctatgc caagcacggc gcggcctgcc
      421 tgagcgtgct gaccgaccag cgcttcttcc agggcgcggc cgaatacttg cagcaggcaa
      481 gagccgcctg tgcgctgccg gtgctgcgca aggacttcat ggtcgacccc taccaggtct
      541 acgaagcgcg cgcgatgggg gccgattgca tcctgctgat cgccgcctgc ctcgccgatg
      601 cgcagatggc cgagctggaa gcctgcgccc aggccctggg catggccgtg ctggtggagg
      661 tgcatgacgg cgccgagctg gaacgcgcgc tgcacctcaa gactcctctg ctgggcatca
      721 acaaccgcaa cctgcgcagc ttcgaggtca cgctggagac cacgctgagc ctccagcgcg
      781 aggtgccggc cgaccgcctg ctcgtcaccg agagcggcat cctcgcccgc gaagacgtgc
      841 gccgcatgcg agaagccggc gtgcacgcct tcctggtcgg cgaggccttc atgcgcgcgc
      901 ccgatccggg tgcggcattg gcagcgcttt tcggcgaacc gtgaagcgct cgccggtgac
      961 tagcgatgcg ggcctgttcg atgacggcga gcccgctggc aatcgtctga gcgagccgct
     1021 ggacgcctgc ttcgatcgcg tgcagggcgc ctggcgcgag gtgacgcagc gctttcgcga
     1081 ctcggtgccc ggtgcggcac tgctgggctt tgtcgatgca agggtgaagg ccggtgcggt
     1141 cgtctatccg gccactgtct tccgcgcgct ggagctcatg ccgctggccg ctgtgcgcgt
     1201 cgtgatcctt gggcaggatc cctaccacgg cgccggccag gcccagggcc tggccttctc
     1261 ggtgccctcg cacttgaaaa aactgccgcc cagcctgcgc aacatctacg ccgaagtggc
     1321 ggccgatctc ggcggctcgc cggcacggga tggcgacctg agccgctggg cgaagcaagg
     1381 cgtgctgctt ctgaacacga ctctgaccgt ggaagacggg cagccgcaca gccatgccgg
     1441 gcgcggctgg gaggtgctta gcgacgcgct ggtcgccgcc gtgtcccagc aggcccagcc
     1501 cagcgtcttc atgttgtggg gcgcgccggc gcagcgcaag cgcgcgctga tcgacgaggg
     1561 ccggcacctg gtgctgacgg ccaatcatcc ttcgccgctg tcggcgcgcc gcccgcccca
     1621 gcccttcatc ggctgccgcc acttcagcca ggcgaacgat tggctgctgc ggtatcgcgc
     1681 gcctgaagcg gcggtcgcgt ggtgagctgc gtcagcgcaa ggcttccatg aacgtctggg
     1741 cataagcgcc gcgcggctgc gccagcatcg agctgcagcg gtccgagacc tcggtcgcat
     1801 cacccgggca gggtgcatcc cggtccagcg accagaaatg caggccggcg agcttgttct
     1861 cgcgcacgaa cttggcgacg agccgcgcat cgtccagcgt gaacacgttc tccttgacgt
     1921 cgttcactcc gatcattggc gtcagctcga tctggctgaa gggcaggccg taccgcgtat
     1981 gcaggttgtg tgcggcctgg atggccgagc ggcccatgtc gcagcgcccg gcagcgacca
     2041 cgcaggccga gggcgaggcg gcgccgtagt ccattaccat cagattgatc gtgtaggcct
     2101 tcaggccgtg gcggcgtatg gattgcagca cccgctgccc cagcgcattc aggctggcgc
     2161 cgctgccgtc gctggccgcg aaagtaggca gcgtgaaact gaacctcagt ttcgggtgac
     2221 gggcctgggc cgccttcacg cgccgcacca gcgcggcgat ctgctcggag gtctgtccgc
     2281 tctcgatgtc gaggtcgaag ccgatcaggt gcgacgaggc gtagcgcgcg atgaagcgct
     2341 ccatgcccgc atcgctgccg cagttgaaga cattgccttg cccgccggtc gatacgacgt
     2401 agtcgatatc ggcgcggacg agttcggcga cattggtgtc agcgaaggcc tgcgcatcat
     2461 ggccggccca gttctcttcg ccgcattcgc ccctcgcgaa cgccagggtc aacgtcgtcg
     2521 gtgcggcggc tgccggcgta tcggcgtcga tcgcacggag gggcgagatc ctgtgggtgc
     2581 cggcatctaa gccgatcgtg atgtccttgt aagggctgta gacgaagccg ggccatgcgg
     2641 cctcaccggc tgcggctgac aggcaggtgg cggccgcaag ggcgcaaatc agcgcctgtg
     2701 ccttcaggat tcggtagctg ttcaatcgat gcctcgctgc acaagcatgc acgaaaaaag
     2761 gggagcgtcg ccgctcccct
//
LOCUS       DASUCY010000432         2717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_941384, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000432 DASUCY010000000
VERSION     DASUCY010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941384"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="VFV25_08615"
     CDS             complement(<1..731)
                     /locus_tag="VFV25_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY1393427.1"
                     /translation="MSCHADEAKLVVGSKRFTESYILGEIVAQTARPYAPVEHRPGMG
                     NTAVVFEALKAGAIDVYPEYLGTLDAEILKHPKPTDIATMQRELAPLGLGVGVPLGFA
                     NGYALALRADEAQRLQLKHISDLRGHPELVLGLSHEFLGRADGWPGLAARYGLPQRPL
                     GIDHGIAYEALAAGRIAATDIYSTDAKIASLGLAVLDDDLHYFPRYDAVLLYRLDAAQ
                     RSPAAWRAIAGLEGRISPAKMIEMNG"
     gene            complement(796..2160)
                     /gene="radA"
                     /locus_tag="VFV25_08620"
     CDS             complement(796..2160)
                     /gene="radA"
                     /locus_tag="VFV25_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEY1393428.1"
                     /translation="MAKDKTVYTCSECGGTSPKWLGKCPSCGAWNTLEETIAESSSPA
                     KNRFQALARSQPVATLSEIEVGEVARTPTGLEELDRVLGGGIVSGGVVLIGGDPGIGK
                     STLLLQAVDALGAQMKVLYVTGEESGAQVALRAQRLGLVGTQVRVLAEINLEKILATI
                     AAEQPAFCVIDSIQTLYSEQLTSAPGSVAQVRECAAQLTRTAKAGGTSMVLVGHVTKD
                     GSLAGPRVLEHIVDTVLYFEGDTHSAFRLVRAFKNRFGAVNEIGVFAMTERGLKGVTN
                     PSAIFLSTHNEPVPGSCVLVTLEGTRPMLVEIQALVDGSGPSPRRLSVGLERDRLAML
                     LAVLHRHAGIASFDQDVFVNAVGGVRISEPAADLSVMLAIQSSLRGKPLPRGFIAFGE
                     VGLAGEVRPAPRGQERLKEAAKLGFDVAIVPKANAPKKPPEGLTVHAVDRIEQAIDLA
                     RTLA"
     gene            2305..>2717
                     /locus_tag="VFV25_08625"
     CDS             2305..>2717
                     /locus_tag="VFV25_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEY1393429.1"
                     /translation="MISLGFLITSLVVVLIPGTGVIYTVSAGLAQGRRASVYASLGCT
                     AGIVPHLIATVLGLAAVMHASALAFQALKYAGVLYLFYVAYATWRDKSAFTVDGSVSR
                     TSALGLVTKAFLLNILNPKLTIFFLAFLPQFVDPAA"
BASE COUNT          411 a          956 c          891 g          459 t
ORIGIN      
        1 ccgttcattt cgatcatctt cgcgggactg atgcgccctt cgagccccgc gatcgcgcgc
       61 caggcggcgg gagagcgctg cgccgcgtcg agccggtaca gcagcaccgc gtcgtagcgc
      121 gggaagtaat gcaggtcgtc gtcgagcacc gccaggccca gcgaggcgat cttggcgtcg
      181 gtcgaataaa tgtctgtcgc cgcgatgcgc ccggctgcca gcgcctcgta ggcgatgccg
      241 tggtctatgc ccagcggtcg ctgcggcaac ccatagcgcg ctgccagccc cggccagcca
      301 tcggcacggc cgaggaattc gtgcgacagg cccagcacca gctcggggtg gccgcgcaga
      361 tcgctgatgt gcttgagctg caaacgctgc gcctcgtcgg cgcgcaaggc cagcgcatag
      421 ccgttggcga agcccagcgg cacgcccacg ccgaggccca gcggcgccag ctcgcgctgc
      481 atcgtcgcga tgtcggtggg cttgggatgc ttgaggatct cggcgtccag cgtgccgagg
      541 tattcgggat agacgtcgat cgcgccggcc ttcagcgcct cgaagaccac ggccgtgttg
      601 cccatgccgg ggcggtgctc gaccggggcg tagggccgcg ccgtctgcgc gacgatctcg
      661 ccgaggatgt aggactcggt gaagcgcttg gagccgacga cgagcttggc ttcgtcggcg
      721 tggcagctca tggccaccac cgccagcgcg aacgcaaagg ctgttgcaaa gcggctgcgg
      781 cgcgacatgt gaggctcagg cgagagttcg agccaggtcg atcgcctgct cgatgcgatc
      841 caccgcgtgc accgtgagcc cctcgggcgg cttcttcggc gcattggcct tgggcacgat
      901 cgcgacatcg aagcccagct tggccgcctc cttcagccgc tcctggccgc gcggcgccgg
      961 ccgcacctcg ccggccaggc cgacctcgcc gaaggcgatg aagccgcgcg gcagcggctt
     1021 gccgcgcagc gagctctgga tcgccagcat cactgatagg tcggccgccg gctcgctgat
     1081 gcgcacgccg cccacggcat tgacgaacac gtcctggtcg aagctggcta tgcctgcgtg
     1141 ccggtgcagc acggccagca gcatcgccag ccggtcgcgc tccaggccca ccgacaggcg
     1201 ccgcgggctg ggcccactgc cgtcaaccag cgcctggatc tccaccagca tgggccgcgt
     1261 gccttccagc gtgacgagca cgcaggagcc cggcaccggc tcgttgtgcg tggagaggaa
     1321 gatcgcactc gggttggtca cgcccttcaa gccccgctcg gtcatcgcga acacgccgat
     1381 ttcgttgacc gcgccgaagc ggttcttgaa ggcgcgcacg aggcggaagg ccgaatgcgt
     1441 gtcgccctcg aaatagagca ccgtgtcgac gatgtgctcc agcacgcgcg ggccggccaa
     1501 cgagccgtcc ttggtcacat ggcccaccag caccatgctc gtgccgccgg ccttggcggt
     1561 gcgcgtgagc tgggccgcgc attcgcgcac ctgggccacc gagccggggg ccgaagtgag
     1621 ctgctcggaa tacagcgtct ggatcgagtc aatgacgcag aaggccggct gctcggccgc
     1681 gatggtggcg aggatcttct cgagattgat ctcggccagc acgcgcacct gcgtgccgac
     1741 cagccccagc cgctgcgcgc gcagcgccac ctgcgcgccg ctctcctcgc cggtgacata
     1801 gagcaccttc atctgcgcac cgagcgcatc caccgcctgc agcagcagcg tcgacttgcc
     1861 gatgcccgga tcgccgccga tcagcaccac gccgccggaa acgatgccgc cgcccaacac
     1921 gcggtccagc tcctcgagcc cagtgggcgt gcgcgccact tcgccgactt cgatctcgga
     1981 cagcgtggcg accggctgcg acctggccag cgcctggaag cggttcttcg ccggcgaact
     2041 gctctcggcg atcgtctcct cgagcgtgtt ccacgcgccg cagctggggc acttgcccag
     2101 ccacttggga ctggtgccgc cgcattcgga gcaggtgtag acggttttgt ctttggccat
     2161 gggggtggac tttagccttt gggtgcttgc ggacttcccg gccctggccg gctcgtcggt
     2221 ctgactcgca tcgccggagt cggatccgag catcaggcgt cacgaacatg cacaatcgag
     2281 cgcacccatt gcgccggagc catcatgatc agcctcgggt tcctcatcac ctcgctcgtg
     2341 gtcgtgctca ttccgggcac gggggtcatc tacaccgtct ccgcaggcct cgctcaaggt
     2401 cgccgggcaa gcgtctacgc cagcctcggg tgcaccgccg ggatcgtgcc tcacctcatt
     2461 gcaacggtcc tgggcctggc tgcggtcatg catgccagcg cactggcgtt ccaggcgctc
     2521 aagtacgctg gcgttctgta tctcttctac gtggcttatg cgacttggcg cgataaatca
     2581 gcgttcaccg tcgacggcag cgtttctcgg acatcggctc tcgggctggt caccaaggcc
     2641 ttcttgctca atatcctcaa cccgaagctg accatcttct tcctggcctt cctgccgcag
     2701 tttgtcgatc cagcggc
//
LOCUS       DASUCY010000433         4453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_443337, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000433 DASUCY010000000
VERSION     DASUCY010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443337"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="VFV25_08630"
     CDS             <1..711
                     /locus_tag="VFV25_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HEY1393430.1"
                     /translation="TLYERSRDWHAAVETARQLEAAGAGSFASRIANYWCEIALEADA
                     RKQPDEAEAALRKAREAAPQAARPLVLSGQRLARQGRHEQAIQAWSELMAIQPAAFSL
                     VAADFANSAVACQQQEHALVQLQALYARVPSVDALQAMTKLDNDAIARREKLVAHLQK
                     QPSLSAALGLLQERLANGRPLANEEIERLQQAISTAAKPLQRYRCAACGFEGQHYFWQ
                     CPGCMSWDSYPPHRLEDQ"
     gene            708..1652
                     /gene="rfaE1"
                     /locus_tag="VFV25_08635"
     CDS             708..1652
                     /gene="rfaE1"
                     /locus_tag="VFV25_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HEY1393431.1"
                     /translation="MNPQQQILSRERLAAARVLVVGDAMLDRYWFGAVDRISPEAPVP
                     VVRVEREEERLGGAANVALNVKTLGAQATLLTVVGNDEPARKLKGLLEREGVATLLGS
                     DPQLYTIVKLRVIGRSQQLIRVDFENQPDHEVLVQMLADYERALASHDAVLFSDYGKG
                     GLTHIPRMIELARAAGKPVLVDPKGSDYARYAGATAITPNRAELAQVIGAWPDEATLH
                     KRAQALRDEFKLQGLVLTRSEEGMSLFDAGGHFQVPAQAREVFDVTGAGDTVIATLAA
                     MLACGLSLREAVPHANRAGGIVVGKFGTASVSYEELFA"
     gene            1649..2644
                     /gene="rfaD"
                     /locus_tag="VFV25_08640"
     CDS             1649..2644
                     /gene="rfaD"
                     /locus_tag="VFV25_08640"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HEY1393432.1"
                     /translation="MRVVVTGAAGFIGANLVQGLNKLGIDDVIAVDNLTNGPKFKNLL
                     GARISDYFDKTDFYARFTRGEFGKVDAVFHEGACSDTMEHNGRYMLDTNYRCSKDLLD
                     GCMAQGTRLLYASSAATYGASSTFREEPAWEQPLNVYGYSKLLFDQVVRRHLASAPVQ
                     IAGFRYFNVYGPREQHKGRMASVAFHHYQQFRDKGQVQLFGEYGGYGPGQQQRDFVFV
                     DDVVAVNLWFLQNPQAGGIFNLGSGRAQPFNDVAHATVNACRALKGEAPLTLEQQVRQ
                     GLVSYIPFPDALVGKYQCHTQADLGTLRGVGCTHGFSDVATGVQAYVAWLAKQGT"
     gene            2681..2947
                     /locus_tag="VFV25_08645"
     CDS             2681..2947
                     /locus_tag="VFV25_08645"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEY1393433.1"
                     /translation="MFKKKLLLALAALMLALPALATEVNQASQAELERIKGIGPTLSD
                     KILDERAKSEFKDWDDLMARVKGMGARSSRRLSDAGLTVNGGLW"
     gene            complement(3032..4291)
                     /locus_tag="VFV25_08650"
     CDS             complement(3032..4291)
                     /locus_tag="VFV25_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY1393434.1"
                     /translation="MRSTQPITGLVLTGGGARAAYQAGVLKAIVRIRRDCRAGRVRNL
                     FPVITGTSAGAINAAALACGADHFERAVERLAAVWRDFHAERIYRADSFGVIRSGAKW
                     LTMFSVGWALARWRRARPRSLLDNAPLEHLLNELVPLQRLPALLHGGQLRALAITASS
                     YSSGEHVIFYDAGDGIEPWTRSLRVAVRQDITRQHLMASSALPFVFPAVQLPFKGSLE
                     YFGDGSMRQVAPISPAVHLGAERILVIGAGRLQEPPDRRPPHHEYPPLAQIAGHALSS
                     IFLDSLAVDIERLQRINHTLSLLTPEARQRTSLRPIDVLVIAPSQRVDDIAARHLHSM
                     PPAVRAMLRALGVSGEGESARGAALASYLLFEPGFTRELMALGESDTLARRAEVERFF
                     GWTPPSERLARERKGDVAVPTAAQASE"
BASE COUNT          751 a         1532 c         1528 g          642 t
ORIGIN      
        1 acgctctacg agcgctcgcg cgactggcat gcggcggtcg agaccgcgcg ccagctcgag
       61 gccgccggcg ccggctcctt cgccagccgc atcgccaact actggtgcga gatcgcgctg
      121 gaggccgacg cgcgcaagca gccggacgag gccgaggccg ccttgcgcaa ggcgcgcgaa
      181 gccgcgccgc aggcggcgcg cccgctggtg ctcagcggcc agcgcctggc ccgccagggc
      241 aggcatgaac aggccatcca ggcctggtcc gagctgatgg cgatccagcc ggcagccttc
      301 tcgctggtgg cggcggactt cgccaacagc gccgtggcct gccagcagca ggagcatgcc
      361 ctggtgcagc tgcaggccct gtacgcgcgc gtcccttcgg tcgacgccct gcaggcgatg
      421 acgaagctgg acaacgacgc catcgcgcgc cgcgagaagc tggtcgccca tttgcagaag
      481 cagccttcgc tgtcggctgc cctgggcctg ctgcaagagc gcctggccaa cggcaggccg
      541 ctcgccaacg aggagatcga gcgcctgcaa caagccatca gcacggcagc caagccgctg
      601 cagcgctatc gttgtgcggc ctgcggcttc gagggccagc attatttctg gcaatgccca
      661 ggttgcatga gctgggacag ctatccgccg catcggctgg aagaccaatg aatccacagc
      721 aacagatcct gagccgcgaa cgcctggccg ccgcacgcgt gctggtggtg ggcgacgcga
      781 tgctcgaccg ctactggttc ggcgcggtgg accgcatctc gcccgaggct ccggtgccgg
      841 tggtgcgcgt cgagcgcgag gaagagcgcc tgggcggcgc cgccaatgtc gcgctgaacg
      901 tgaagacgct gggcgcccag gcgacgctgc tcaccgtggt cggcaacgac gagccagcac
      961 gcaagctcaa gggcctgctg gaacgcgagg gcgtggcgac gttgctgggc agcgacccgc
     1021 agctctacac catcgtcaag ctgcgcgtca tcggccgctc gcagcagctg atccgcgtcg
     1081 atttcgagaa ccagcccgac catgaggtgc tggtccagat gctggccgac tacgagcgcg
     1141 cgctggcctc gcacgacgcc gtgctgttct ccgactacgg caagggcggg ctcacgcaca
     1201 tcccgcgcat gatcgagctg gcccgcgcgg cgggcaagcc ggtgctggtc gatcccaagg
     1261 gcagcgacta tgcgcgctat gccggtgcca ccgccatcac gcccaaccgc gccgagctgg
     1321 cccaggtgat cggcgcctgg cccgacgagg ccacgctgca caagcgcgcg caggcgctgc
     1381 gcgacgagtt caagctgcaa ggcctggtgc tgacgcgctc cgaggagggc atgtcgctgt
     1441 tcgatgccgg cggccatttc caggtgcccg cccaggcgcg cgaagtcttc gacgtgaccg
     1501 gcgccggcga caccgtgatc gcgacgctgg ccgcaatgct ggcctgcggg ctcagtctgc
     1561 gcgaagccgt gccgcatgcg aaccgcgccg gcggcatcgt ggtcggcaag ttcggcacgg
     1621 ccagcgtcag ctacgaggag ttgtttgcat gagagtcgtc gtcaccggcg ccgcgggatt
     1681 catcggggcc aatctggtcc agggcctcaa caagctcggc attgacgacg tcatcgccgt
     1741 cgacaacctg accaatggcc ccaagttcaa gaacctgctg ggcgcacgca tcagcgacta
     1801 cttcgacaag accgatttct acgcgcgctt cacgcgcggc gagttcggca aggtcgatgc
     1861 ggtgttccac gagggcgcct gctccgacac gatggagcac aacgggcgct acatgctgga
     1921 caccaattac cgctgctcca aggatctgct ggacggctgc atggcccagg gtacgcggct
     1981 gctctacgcc tcgtcggctg cgacctatgg cgcgagcagc accttccgcg aggagccggc
     2041 ctgggagcag ccgctcaatg tctacggcta ctccaagctg ctgttcgatc aggtggtgag
     2101 gcgccacctg gcctcagcgc cggtgcagat cgcgggcttc cgctatttca acgtctacgg
     2161 cccgcgcgag cagcacaagg gccgcatggc ctcggtggcc ttccaccact accaacaatt
     2221 ccgcgacaag gggcaggtgc agctcttcgg cgagtacggc ggctatggcc ccggccagca
     2281 gcagcgcgac ttcgtcttcg tcgacgacgt ggtcgcggtg aacctgtggt tcctgcagaa
     2341 cccgcaggcc gggggcatct tcaacctcgg cagcgggcgc gcccaaccct tcaacgatgt
     2401 ggcgcatgcc accgtcaatg cctgccgcgc cctcaagggc gaagcgccgc tgacgctgga
     2461 gcagcaggtg aggcagggtc tggtgagcta catccccttc ccggacgcgc tggtcggcaa
     2521 gtatcaatgc cacacgcagg ccgacctcgg cacactgcgc ggcgtgggct gcacgcacgg
     2581 cttttctgac gtcgccaccg gcgtgcaggc ctatgtggcc tggctggcta agcaggggac
     2641 ctgagaccaa cgctgctcgt cattcatcgg ggagaacaac atgttcaaga agaaattgct
     2701 gctggccctg gcggccctga tgctcgccct gcccgccctc gcgacggaag tgaaccaggc
     2761 cagccaggcg gagctcgagc gcatcaaggg cataggccca acgctctcgg acaagatcct
     2821 ggacgagcgg gccaagagcg agttcaagga ctgggacgac ctgatggccc gcgtcaaggg
     2881 catgggcgct cgcagttcgc gcaggctgtc cgacgccggt ctgaccgtca atggcggcct
     2941 atggtgaccc gaacagtcgg aacgccgcaa aggcagccgc acagccagcc gcttcaggcg
     3001 cccagaccgg taagtagtca ggccgcagcc ctcactcgga ggcctgcgcc gcggtcggta
     3061 ccgcgacatc gcccttgcgc tcgcgcgcca gccgctcgga cggcggcgtc catccgaaga
     3121 agcgctccac ctcggcgcgc ctggcgagcg tgtcgctttc acccagcgcc ataagctcgc
     3181 gcgtgaaccc gggctcgaac agcagatagc tcgccagggc cgcaccgcgg gcgctctcgc
     3241 cttcgccgga cacgcccagg gcgcgcagca tcgcgcgcac cgccggcggc atgctgtgca
     3301 agtgccgcgc cgcaatgtcg tccacgcgct ggctgggtgc gatcaccagc acgtcgatgg
     3361 ggcgcaggct ggtacgctgg cgcgcctcgg gcgtcagcag cgacagcgtg tgattgatgc
     3421 gctgcagccg ctcgatgtcg accgccagcg agtcgaggaa gatgctcgac agcgcatggc
     3481 ccgcgatctg ggccaagggc gggtactcgt ggtgcggagg gcgccggtcg ggcggctcct
     3541 gcaggcgccc ggcgccgatc acgaggatgc gctcggcgcc caggtgcacg gcgggcgaga
     3601 tgggcgccac ctggcgcatc gagccgtcgc cgaagtattc caggctgccc ttgaaaggca
     3661 gttgcaccgc cgggaacacg aaaggcaggg cggaggacgc catcaagtgc tggcgtgtga
     3721 tgtcctggcg cacggcgacg cgcagcgagc gcgtccaggg ttcgatgccg tcgcccgcgt
     3781 cgtagaagat cacatgttcg cccgagctgt agctcgaggc ggtgatcgcc agtgcgcgca
     3841 gttggccgcc atgcagcaaa gcaggcaggc gctgcagcgg caccagctcg ttcagcagat
     3901 gctccagcgg cgcgttgtcc agcagcgagc gcggcctcgc gcggcgccag cgcgccaggg
     3961 cccagcccac cgaaaacatg gtgagccact tggcgccgct gcggatcacg ccaaaggaat
     4021 cggcgcgata gatccgctcg gcatggaagt cgcgccagac cgccgccagc cgctcgacgg
     4081 cccgctcgaa atggtcggcg ccgcaagcca gggccgccgc attgatggcc cccgctgaag
     4141 tgccggtgat gaccggaaag aggttgcgca ctcgcccggc gcggcagtcg cggcgtatgc
     4201 ggacgatagc tttgagtacg ccggcctggt aggccgcgcg agccccaccg ccggtcagga
     4261 cgaggccggt gatgggctgc gtgctgcgca tgcagcgcag tttagggtcg gccgacaacc
     4321 tggacgatca ggatcggccc cgaggctagt tgaaggagcc gacctcgctc agtcatcctc
     4381 gtgaccatgg ccatgcttgc gcccatggtc gccccggtcg ccatgcccgc ggccacggcc
     4441 gcgatcgcca tct
//
LOCUS       DASUCY010000434         2232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_138139, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000434 DASUCY010000000
VERSION     DASUCY010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138139"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1516)
                     /locus_tag="VFV25_08655"
     CDS             complement(<1..1516)
                     /locus_tag="VFV25_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEY1393435.1"
                     /translation="MHDANEVHDPGRPWLARLDAALGLLVEVPAALLVLADIGVLFWG
                     VIARFVLHRPLVWSDELASILFLWLAMLGSVVALRRGEHMRMTALVSKAGRERRALLE
                     AVATSACLAFLGLVAWPAWEYAAEELVVTTPALEISNAWRAAALPVGIALMTVFAALR
                     LLRVSNFKQSLQAVAIVAAIVGAFWGAGPVFEELGRLNLLIFFVGVAAGCVFAGIPIA
                     FAFGLGTFGYLALTTGTPLPVIVGRMDEGMSHLILLAVPLFVFLGLLIEMTGMARAMV
                     AFLAGLLGHVRGGLSYVLVGAMYLVSGISGSKAADMAAIAPALFPEMKKRGAKEGDLV
                     ALLAATGAQTETVPPSLVLITIGSVTGVSIAALFTGGLLPAVVLGLVLCMVVWFRYRH
                     EDLSQVQRTPWKEVGRLFAIAVPALALPFIIRAAVVEGVATATEVSTIGIAYAVLAGL
                     FIYRQFDWRRIGPMLVNTAALSGAILLIIGTATAMAWGLTQSGFSRWLAEIMAAL"
     gene            complement(1573..>2232)
                     /locus_tag="VFV25_08660"
     CDS             complement(1573..>2232)
                     /locus_tag="VFV25_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEY1393436.1"
                     /translation="YDTVWKAMDGELGAHVRAQIAKANLVAMDKIWDNGFRQITSSTR
                     PITTADDLKGMKIRVPPSPLWTSMFKSFDAAPASINFSEVYSALQTKVVEGQENPLAI
                     IATAKLYEVQKYCSLTNHMWDGFWFLANRKAWERLPADLRAIVAKHINEAGMKERTDV
                     AALNATLQKDLAEKGLVFNQPKADSFRERMRKAGFYAEWKARFGDEAWALLERSTGKL
                     A"
BASE COUNT          397 a          773 c          726 g          336 t
ORIGIN      
        1 gcagcgccgc catgatctcg gccagccaac gtgagaagcc cgattgcgtg agcccccagg
       61 ccatggccgt cgcggtgccg atgatcagca ggatcgcgcc cgagagcgcc gccgtgttga
      121 ccagcatcgg gccgatgcgc ctccagtcga attgccggta gatgaacaga ccggcgagca
      181 cggcatacgc aatgccgatg gtcgagacct cggtcgccgt ggccacgcct tcgaccaccg
      241 ccgcgcggat gatgaagggc aaggccaggg ccgggacggc gatggcgaac agccggccca
      301 cctccttcca cggcgtgcgc tgcacctggc tcaggtcttc atggcggtag cggaaccaga
      361 ccaccatgca cagcaccagg ccgagcacca ccgccggcag caggccgccg gtgaacagcg
      421 ccgcgatcga cacaccggtg accgatccga tcgtgatcag caccaggctg ggcggcacgg
      481 tctcggtctg cgcgccggtg gccgcgagca gggccaccag gtctccctcc ttcgcgccgc
      541 gctttttcat ctcggggaac agcgccggag cgatggccgc catgtcggcc gccttggagc
      601 cggagatgcc cgagaccagg tacatggccc cgaccagcac gtacgacagg ccgcccctca
      661 catggccgag caggccggcc aggaaggcga ccatcgcgcg ggccatgccg gtcatctcga
      721 tgagcaggcc gaggaagacg aacagcggca ccgccagcag gatcaggtgc gacatgcctt
      781 catccatgcg gccgacgatc accggcagcg gcgtgcccgt ggtgagtgcc aggtagccga
      841 aggtgccgag gccaaaggcg aaggcgatcg gaatgccggc gaaaacgcag ccggctgcca
      901 cgccgacaaa gaagatcagc aggttcaggc gtcccagttc ctcgaacacc ggaccggcgc
      961 cccagaaggc cccgacgatg gccgccacga tggccaccgc ctgcagcgac tgcttgaagt
     1021 tcgagaccct cagcaggcgc agcgcagcga aaactgtcat cagcgcgatg cccaccggca
     1081 gcgcggcggc gcgccaggca ttggagatct cgagcgccgg cgtggtgacg accagctcct
     1141 ccgcggcgta ctcccaggct ggccaggcca ccaggcccag gaacgccagg caggcgctcg
     1201 tggccacggc ttcgagcagg gcccgccgct ctcggccggc cttgctgacc agcgccgtca
     1261 tgcgcatatg ctcgcctcgg cgcagggcca cgaccgagcc cagcatcgcc agccacagga
     1321 acaggatgga cgctagctcg tccgaccaca ccagcgggcg gtgcaggacg aagcgcgcga
     1381 tcacgcccca gaacagcacg ccgatgtcgg ccagcacgag cagcgcggcc ggcacttcga
     1441 ccagcaggcc cagcgcggca tcgaggcggg ccagccacgg ccgtcccgga tcatgcactt
     1501 cgttcgcgtc atgcatgtgg gccccaggta ttggtttggc gatggtcttg accggatgcg
     1561 cggtcgcgcc gctcatgcca gcttacctgt gctgcgctcc aggagcgccc aggcctcgtc
     1621 tccgaaccgg gccttccatt cggcatagaa gccggccttg cgcatgcgct cccggaagct
     1681 gtccgccttg ggttgattga acaccaggcc cttttcggcg agatccttct gcagcgtggc
     1741 attcagtgct gcgacgtcgg tgcgctcctt catgcccgcc tcgttgatgt gcttggcgac
     1801 gatggcgcgc agatcggcgg gcaggcgctc ccaggccttg cggttggcga ggaaccagaa
     1861 gccgtcccac atgtggttgg tgagcgagca gtatttctgc acctcataca gcttggccgt
     1921 tgcgatgatc gccagcggat tctcctggcc ctcgacgacc ttggtctgca gcgccgaata
     1981 cacctcgctg aagttgatcg atgccggtgc ggcgtcgaac gacttgaaca tcgacgtcca
     2041 cagcggcgat ggcggcacgc ggatcttcat gcccttcaag tcgtccgctg tcgtgatcgg
     2101 ccgggtcgac gaagtgatct gcctgaagcc gttgtcccag attttgtcca tcgcgaccag
     2161 gttggccttg gcgatctgcg cccgtacatg ggcaccgagt tcgccatcca tcgctttcca
     2221 caccgtgtcg ta
//
LOCUS       DASUCY010000435         2059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1069983, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000435 DASUCY010000000
VERSION     DASUCY010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069983"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="VFV25_08665"
     CDS             complement(<1..406)
                     /locus_tag="VFV25_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006402676.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEY1393437.1"
                     /translation="MHDKILILDFGSQVTQLIARRVREAHVYCEIHPNDVSDAFIREF
                     APKGIILSGSHASTYEAHDLRAPQAVWDLGVPVLGICYGMFTMAVQQGGKVEASTHRE
                     FGYAEVRAHGHTRLLAGIQDFSTTEGHGMLKVW"
     gene            complement(446..1720)
                     /locus_tag="VFV25_08670"
     CDS             complement(446..1720)
                     /locus_tag="VFV25_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1393438.1"
                     /translation="MTSPPSVDSKRRQAAMPFIMLAVLIDMVSIGLIVPVLPPLVGTF
                     TSSPAEQTWWFGVISFTFGVANFFGSPILGALSDRYGRRPVLLIGFSGLALSFFVTGM
                     ATALWMLIAVRLVSGATMGNAAVANAYVADITAPEDRAKRFGMLGAMFGMGFILGPVL
                     GGLLGDINLQLPFFVAGGLAVLNWLYGYFVLPESLPAERRHPINWKSANPVSSLRRLS
                     QLKGAGPLVAVIGLSGLAQFMLQTTWVLYTHFKFGWGPSQNGWSLFAVGVMSSLVQGV
                     LLGRLLQRFSAQRLAVMGLLSSAICFTLWGAATQGWMMYAVIFCNLLGFTVVASIQSL
                     ISNAADAKTQGQTMGAVASLNSLMAVAAPVLGPALLAFVSHLPHGDWRIGAPYYFCAA
                     LQAVSMLLALRHFSAMKRGAALLPPGSAEPSA"
     gene            complement(1789..>2059)
                     /locus_tag="VFV25_08675"
     CDS             complement(1789..>2059)
                     /locus_tag="VFV25_08675"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEY1393439.1"
                     /translation="FQDNTGANPNADKLVPEGIEGRVPYKGSVVTILFQMAGGLRASM
                     GYCGCATVDEMRERAEFVEITAAGIRESHVHDVQITKEAPNYRAE"
BASE COUNT          383 a          684 c          694 g          298 t
ORIGIN      
        1 tccacacctt gagcatgccg tggccctcgg tggtgctgaa atcctggatg ccggccagca
       61 gccgggtgtg gccatgcgcc ctcacctcgg catagccgaa ctcgcggtgc gtgctggcct
      121 cgaccttgcc gccctgctgc accgccatcg tgaacatgcc gtagcagatg ccgagcaccg
      181 gcacgcccag atcccacacc gcctgcggag cgcgcagatc gtgcgcttcg taggtgctgg
      241 catggctgcc cgaaaggatg atgcccttgg gcgcgaactc gcggatgaag gcatcggaca
      301 cgtcgttggg atggatctcg caatacacgt gcgcttcgcg cacgcggcgt gcgatcagct
      361 gggtcacttg cgaaccgaag tcgagaatga ggatcttgtc gtgcatggtg attccgaaac
      421 ggcctctgtc aatgtctttg cgtattcatg ccgaaggctc cgcgctgccc ggcggcagca
      481 gcgccgcgcc gcgtttcatc gccgagaagt ggcgcagcgc cagcagcatg gagacggcct
      541 gcagcgcagc gcagaagtag tacggcgcgc cgatgcgcca atcgccgtgc ggcaagtgcg
      601 atacgaaggc cagcagcgcc ggccccagca cgggcgcagc caccgccatg aggctgttca
      661 acgaagccac ggcgcccatg gtctggccct gggtcttggc gtcggccgcg ttggagatca
      721 ggctctggat cgaggccacc acggtgaagc ccagcaagtt gcagaagatc accgcataca
      781 tcatccagcc ctgggtggcc gcgccccaca gcgtgaagca gatcgccgag gacagcaggc
      841 ccatcacggc caggcgctgg gccgagaagc gctggagcag ccgccccagc agcacgccct
      901 gcaccagcga ggacatgacg cccaccgcga acagcgacca gccgttctgg ctcggccccc
      961 agccgaactt gaaatgggtg tagagcaccc aggtggtctg cagcatgaac tgcgcgaggc
     1021 cgctcaagcc gatcaccgcc accagcggcc ctgcaccctt gagctgcgag agccgccgca
     1081 gcgagctcac ggggttggcc gatttccagt tgatggggtg tcggcgctcg gccggcagcg
     1141 attcgggcag cacgaaatag ccgtacagcc agttgagcac cgccaggccg cccgccacga
     1201 agaagggcag ctgcaaattg atgtcgccca gcaagccgcc cagcaccggc ccgaggatga
     1261 agcccatgcc aaacatcgcg cccagcatgc cgaagcgctt ggcgcgatcc tcgggcgccg
     1321 tgatgtcggc cacataggcg ttggcgaccg cggcattgcc catggtcgcg ccgctcacca
     1381 ggcgcacggc gatcagcatc cacagcgcgg tggccatgcc ggtcacgaag aagctcaggg
     1441 ccaggccgct gaagccgatc agcagcaccg gccggcgtcc atagcgatcg gacagcgcgc
     1501 ccaggatggg cgagccgaag aagttggcga cgccgaaggt gaaggagatg acgccgaacc
     1561 accaggtctg ttcggccggc gacgaggtga aggtgccgac cagcggcggc agcaccggga
     1621 cgatgaggcc gatcgacacc atgtcgatca gcacggccag catgatgaag ggcatggccg
     1681 cctggcggcg cttggagtcg acagacggag ggctcgtcac gggatttctc tcaaaaaccg
     1741 gcccgcccgg gcggagccgg gcggggcgcg ggtatacagg caggctgctt actcggcgcg
     1801 atagttcggc gcctccttgg tgatctgcac gtcgtgcaca tggctctcgc gtatgccggc
     1861 ggccgtgatc tcgacgaact cggcgcgctc gcgcatctcg tccacagtgg cgcagccgca
     1921 gtagcccatg gaggcgcgca gcccaccggc catctgaaac aggatcgtga ccaccgagcc
     1981 cttgtagggc acgcggcctt cgatgccctc gggcaccagc ttgtcggcgt tggggttggc
     2041 gccggtgttg tcctggaag
//
LOCUS       DASUCY010000436         3219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1455470, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000436 DASUCY010000000
VERSION     DASUCY010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455470"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VFV25_08680"
     CDS             complement(<1..245)
                     /locus_tag="VFV25_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY1393440.1"
                     /translation="MAAADLEAAYALSAELRWPHRFADWEQVFRLGEGLVAERDGVVV
                     GTGLRWRWGPRHATIGLIIVAPACQGRRIGHRLMSGLL"
     gene            516..1997
                     /locus_tag="VFV25_08685"
     CDS             516..1997
                     /locus_tag="VFV25_08685"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013210083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEY1393441.1"
                     /translation="MQVFHPDEVNVPSGHFMGGRLVPDAGRIDVLRPSDARHHATLPL
                     ADADTVDTIVRDAWSAWRSSDWARRAPRERARVLRRWADLIEADVARLAPLEAVCSTR
                     PVRDAAAWDIPFTAEGLRFFAEYADKLGGDVAATRSDHLGMVIAEPYGVVGAITPWNF
                     PLVMVSWKVGAALAAGNAVVLKPSELTPFSALRLAELAVQADLPAGLFNIVQGDGRTT
                     GEALSRHPLISKMTFTGSTRTGAAIMASCAQHGPKPVTLELGGKSPQLVFDDAPDIDR
                     LAGMIAGSITGNAGQVCVAGSRLIVQQGIAQALVDRIAQRFAAHRPGATWDDAATLPP
                     IISRPQAGRIAGILERAKASGAALRCGGGLYEGGPGGAYFQPTLVEGVDASSPAVCEE
                     IFGPVLTVQTFADEEEGLALAAHEHYGLASGVHTADIGRALRAMRGISAGTVWINRYG
                     RSSDFVIPTGGYHQSGIGKDLGRQAVEANLRFKSVLIDFAAVH"
     gene            2203..2868
                     /locus_tag="VFV25_08690"
     CDS             2203..2868
                     /locus_tag="VFV25_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEY1393442.1"
                     /translation="MAFRPKYISFDCYGTLIRMRFDDLARDIFADRIPAERMDQFVAD
                     FTAYRFDEVLGNWKPYETVLKNAVRRLCRKWKLQYLDEEAQRFYDGVPTWGPHDDVPA
                     GLAKVAKEIPLVILSNAMDHQIQQNVARLGAPFYRVYTAQQAQCYKPRLQAFEYMLDS
                     LGCNPEDMLHVSSSLRYDLMSANDIGIKNKVFVGRGHGPGCPAYNYTEIKDIGGLAGV
                     VGL"
     gene            3005..>3219
                     /locus_tag="VFV25_08695"
     CDS             3005..>3219
                     /locus_tag="VFV25_08695"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013210081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY1393443.1"
                     /translation="MKLDSYWTDSAPAFVPAAGNLPAQVDVAIIGAGFTGLSAALALA
                     RSGASVAVLEAGSRIAAEASGRNGGHV"
BASE COUNT          538 a         1140 c         1049 g          492 t
ORIGIN      
        1 agcaggccgc tcatgagccg gtggccgatg cgccgcccct ggcaggccgg ggcgacgatg
       61 atcaggccaa tcgttgcgtg ccgcgggccc cagcgccagc gcaagccggt gccgacgacc
      121 acgccatcgc gctcggccac cagcccctcg cccaggcgga acacctgctc ccagtcggca
      181 aagcgatgcg gccagcgcag ctcggccgaa agcgcgtacg cggcctccag gtcggcggcg
      241 gccatattcc gcagcagaac accgtctgcc gccggcgcgc gttggattcg tgagtgattc
      301 aaggctctta ctcccagcat gatgcggtga tcttgaacag gccgcagccg gcgtcgctag
      361 gacgattttg ctgccccggc caacttgcaa gctttaactg ccgacgcagg gcgattcgca
      421 attcgatgtc gaggcgcgtg catcaacaca cacatgtgcc gcgccatcgc gcctaggatt
      481 tgcgaacgcc ctgtgcactc aatcagagac aaaaaatgca agtgttccat cccgacgagg
      541 tcaacgtgcc atcagggcat ttcatgggag gccggctcgt ccccgatgcc gggcgcatcg
      601 acgtgctgcg gccgtccgat gcccggcacc acgcgacgct gccgctggcc gacgccgata
      661 cggtcgacac catcgtgcgc gatgcctggt cggcttggcg cagcagcgac tgggcgcgcc
      721 gcgcaccgcg cgaacgcgcc cgggtgctgc gccgctgggc cgacctgatc gaagccgacg
      781 tcgcgcggct cgcgccgctc gaggccgtgt gctcgacgcg cccggtgcgc gatgcggcgg
      841 cctgggacat ccccttcacc gccgaaggcc tgcgcttctt cgccgagtac gccgacaagc
      901 tgggcggcga tgtggccgcg acacgcagcg atcacctcgg catggtgatc gccgagcctt
      961 acggcgtggt cggcgcgatc acgccctgga atttcccgct ggtgatggtg tcgtggaagg
     1021 tgggcgccgc gctcgcagca ggcaacgcgg tggtgctcaa gccgtccgag ctgacgccct
     1081 tctcagcgct gcggctggcc gagctggcgg tgcaggcgga cctgccggcg gggctgttca
     1141 acatcgtgca gggcgacggc cgcaccaccg gcgaggcgct gtcgcgccat ccgctgatct
     1201 ccaagatgac cttcaccggc tccacccgca ccggcgcggc catcatggcg agctgcgcgc
     1261 agcacggccc caagccggtg acgctggagc tcggcggcaa gagcccgcag ttggtgttcg
     1321 acgacgcgcc ggatatcgat cgcctggccg gcatgatcgc cggcagcatc accggcaatg
     1381 ccggtcaggt gtgcgtggcc ggctcgcgcc tcatcgtgca gcaaggcatc gcgcaggcgc
     1441 tggtcgatcg catcgcgcag cgctttgctg cgcatcgccc gggcgccacc tgggacgatg
     1501 cggccacgct gccgcccatc atctccaggc cccaggccgg ccgcatcgcc ggcatcctcg
     1561 agcgcgcaaa ggcaagcggc gcggcactgc gctgcggtgg cggcctctac gagggcggcc
     1621 ccggcggcgc ctatttccag ccgacgctgg tcgaaggcgt ggacgcctcg agcccggcgg
     1681 tgtgcgaaga gatcttcggc ccggtgctca cggtgcagac cttcgccgac gaggaagaag
     1741 gcctggcgct ggctgcgcac gagcactatg gccttgcatc gggcgtgcac accgccgaca
     1801 tcggacgcgc gctgcgcgcg atgcgcggca tctcggccgg caccgtctgg atcaaccgct
     1861 atggccgcag cagcgacttc gtgatcccca ccgggggtta tcaccaatcc ggcatcggca
     1921 aggacctggg gcgccaggcg gtggaagcga acctgcgctt caagagcgtg ctgatcgact
     1981 tcgcggcggt gcattgaggc gcatgcatgc acagcccctg ggcacaccgc acgccatgcc
     2041 aaacgccccc ccgcaaacca cagcttttcc cttgcagcga ccccaatacc ccacaacccg
     2101 tcggtcgtcg catgctgcaa tgacagcgta atgaatcccg catcgcaagc accgaacagg
     2161 caccgcgccc cacgctaaac ccgcactcca gaggacgttg acatggcctt ccgccccaaa
     2221 tacatcagct tcgactgcta cggcacgctg atccgcatgc gcttcgacga tctcgcgcgc
     2281 gacatcttcg ccgaccgcat cccggccgag cgcatggatc aattcgtcgc agacttcacg
     2341 gcctatcgct tcgacgaggt gctgggcaac tggaagcctt acgagaccgt gctgaagaac
     2401 gcggtgcgcc ggctgtgccg caagtggaag ctccagtacc tcgacgaaga ggcgcagcgc
     2461 ttctacgatg gcgtgccaac ctggggcccg cacgacgacg tgccggccgg cctggccaag
     2521 gtggccaagg aaatcccgct ggtgatcctg tccaacgcga tggaccacca gatccagcag
     2581 aacgtcgcca ggctgggcgc gcccttctac cgcgtctaca ccgcccagca ggcacagtgc
     2641 tacaagccgc gcctgcaggc cttcgaatac atgctggact cgctcggctg caatcccgaa
     2701 gacatgctgc atgtgtcgtc cagcctgcgc tacgacctga tgtcggctaa cgacatcggc
     2761 atcaagaaca aggtctttgt cggacgcggc cacggccccg gctgtccggc ctacaactac
     2821 accgagatca aggacatcgg cggtctggcc ggggtcgtcg ggctctgacg tggaccaccc
     2881 cctacggcct tcggccgccc ccttcgaggg ggcgacaccg gcggaccggc aaagccggat
     2941 ccgcggtgtc cgcttggctt ctgccgagag ctctggggtg tccaagcgcg gtgaaaccac
     3001 tgagatgaag ctcgattcgt actggactga ttcggccccg gctttcgtgc cggcggcggg
     3061 caatctgccg gcccaggtcg acgtggccat catcggtgcc ggcttcaccg gtttgtcggc
     3121 cgccttggcg ctggcacgca gcggcgccag cgtcgcggtg ctggaggcag gctcccgcat
     3181 cgccgccgag gcctcgggcc gcaacggtgg tcatgtcaa
//
LOCUS       DASUCY010000437         5009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1118124, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000437 DASUCY010000000
VERSION     DASUCY010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 5009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5009
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118124"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /gene="rplU"
                     /locus_tag="VFV25_08700"
     CDS             <1..181
                     /gene="rplU"
                     /locus_tag="VFV25_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEY1393444.1"
                     /translation="ADLKVGTPLVAGASVKATVVAHGKHDKVRIFKMRRRKHYQKHQG
                     HRQTFTELEISAVNG"
     gene            212..469
                     /gene="rpmA"
                     /locus_tag="VFV25_08705"
     CDS             212..469
                     /gene="rpmA"
                     /locus_tag="VFV25_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEY1393445.1"
                     /translation="MAQKKGGGSTRNGRDSQPKMLGVKAFGGQVVSAGSIIVRQRGTK
                     FHAGTNVGTGKDHTLFALVDGQVSFDVKGPQSRKTVNVTAV"
     gene            600..779
                     /locus_tag="VFV25_08710"
     CDS             600..779
                     /locus_tag="VFV25_08710"
                     /inference="COORDINATES: protein motif:HMM:NF039187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HEY1393446.1"
                     /translation="MNRSGIVLIVVGLLLLANNLGWLGFAWLRDWWPLLLVALGLWSV
                     LTHSPADNERKDKQP"
     gene            776..1891
                     /gene="obgE"
                     /locus_tag="VFV25_08715"
     CDS             776..1891
                     /gene="obgE"
                     /locus_tag="VFV25_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEY1393447.1"
                     /translation="MKFVDEAYIDVAAGNGGSGCVSFRREKFIPFGGPNGGDGGRGGH
                     VYAVADRNLNTLVDYRYARRHEARNGESGRGSDQFGAAADDIVLRMPVGTIITDAETG
                     TVVTELLDPGEMVMLAKGGDGGFGNLHYKTSTNRAPRQKTPGWPGEQKKLKLELRVLA
                     DVGLLGMPNAGKSTLIAAISNARPKIADYPFTTLHPNLGVVRVGPERSFVVADVPGLI
                     EGAAEGAGLGHQFLRHLQRTRLLLHIVDLAPFDEGVDPVAQAKSIVAELKKYDPALYD
                     KPRWLVLNKLDMVPAEERAARVKDFVKRFKWKGPVFEISALTREGCEPLIHAIWQHVA
                     TYQTQVVEPDVRFDAPPPVEVPTEPGFLPMPDDPRFR"
     gene            1914..3032
                     /gene="proB"
                     /locus_tag="VFV25_08720"
     CDS             1914..3032
                     /gene="proB"
                     /locus_tag="VFV25_08720"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEY1393448.1"
                     /translation="MSDVLKSARRIVVKVGSSLVTNEGRGLDAEAIGNWCRQLAALAS
                     QGREVVMVSSGAVAEGMKRLGWAKRPKEVNELQAAAAVGQMGLAQIYETKLREHGLGS
                     AQVLLTHADLADRERYLNARSTLLTLLSLKVIPVINENDTVVNDEIKFGDNDTLGALV
                     ANLVEADALVILTDQKGLYSADPRKDPKARFIDVAQAGDPALEQMAGGAGSSIGRGGM
                     ITKVLAAKRAAGSGASTVVAWGRESDVLVRLAAGEAMGTLFIAGTAKQAARKQWMADH
                     LQLRGTLVIDDGAAVKLRDEGKSLLPIGVVEVAGEFHRGDVVAVRNAAGLEIARGLAN
                     YSSAEARLIARKPSSEFERLLGYTAEPEMIHRDNLVLA"
     gene            complement(3094..3681)
                     /locus_tag="VFV25_08725"
     CDS             complement(3094..3681)
                     /locus_tag="VFV25_08725"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEY1393449.1"
                     /translation="MLDRDGFRPNVGIILLNQRNQVFWGKRIRTHSWQFPQGGIKYGE
                     TPEQAMLRELQEEVGLLPEHVRIIARTRDWLRYEVPDHYIRREARGHYRGQKQIWFLL
                     QLTGRDNDMNLRATDHPEFDAWRWTEYWVPLDVVIEFKRDVYQMALTELARFLPRTNH
                     HNRYLRSGMRPHRRETEAGLDSQPAEPHPGDGEPH"
     gene            3812..>5009
                     /locus_tag="VFV25_08730"
     CDS             3812..>5009
                     /locus_tag="VFV25_08730"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEY1393450.1"
                     /translation="MKATQFFISTLKEAPADAEIVSHKLMMRAGFIKRLGAGIYSYMP
                     MGLRVIRKVEGIIREEMNRAGAVELLMPVVQPAELWQESGRFEKYGPELLRIKDRHER
                     DFVIQPTSEEVITDIARQELRSYRQLPKNFYHIQTKFRDERRPRFGLMRGREFTMKDA
                     YSFDRDVEGAGKSYEVMYAAYRKIFDRLGLQYRAVAADTGAIGGDRSHEFQVIADTGE
                     DALVYCPTSDYAANVELAEAVPLIAQRAAPAQALHKTPTPGKATCEEVAELLKLPLVQ
                     TVKSLVLATDVKNEAGDVVRTALWLLLVRGDHELNEVKAGKLPGLKDGYRFATAAEIE
                     QHFGCKPGYLGPIGLKEPVKVIADRTVANMSDFVCGANDEDYHYTGVNWGRDLPEPDL
                     VADIRNA"
BASE COUNT          902 a         1625 c         1678 g          804 t
ORIGIN      
        1 cgcggacctg aaggttggga ctcccttggt ggcgggcgcc tctgtgaagg ccacggtggt
       61 ggcgcatggc aagcacgaca aggtgcgcat cttcaagatg cgtcgtcgca agcactacca
      121 gaagcaccag ggtcatcgtc aaaccttcac cgagctggaa atcagcgcgg tgaacggctg
      181 atagcccgca acgcatccaa ggagctgaaa catggcacag aaaaagggcg gcggttccac
      241 acggaacggc cgtgactccc aaccgaagat gctcggcgtg aaggcgttcg gcggccaagt
      301 cgtgtccgcc ggctcgatca tcgtgcgcca gcgcggcacc aagttccacg ccggcaccaa
      361 tgtgggcacc ggcaaggacc acacgctgtt cgccctggtg gacggccagg tgtccttcga
      421 cgtgaagggc ccgcaaagcc gcaagacggt caacgtcacg gcggtctgag cagtctgagc
      481 gctttgccgc gcatagagtg caagccccgg ctcgccgggg ctttcgcttt ttgggccggt
      541 gcgctcgctc cacgtccgtc cattaccatc gaaaatcacc accgttgccg gagcgtcgca
      601 tgaacaggag cgggatcgtc ctcatcgtcg tcgggctctt gctgctggcc aacaatctcg
      661 gctggctggg cttcgcttgg ctgcgtgatt ggtggccgct gctgctggtc gcgctgggcc
      721 tgtggtccgt cctgacgcac agccctgccg acaacgaacg caaggacaag cagccatgaa
      781 gttcgtcgac gaggcctata tcgacgtcgc ggccggcaac ggcggcagcg gctgcgtgag
      841 cttccgcaga gagaagttca tccccttcgg cggtcccaac ggcggcgacg gcgggcgcgg
      901 cgggcatgtc tacgcggtgg ccgaccgcaa tctcaacacg ctggttgatt accgctacgc
      961 gcgccgccac gaagcgcgca acggcgagtc cgggcgcggc tccgaccagt tcggcgccgc
     1021 ggccgacgac atcgtgctgc gcatgccggt gggcaccatc atcaccgacg ccgaaaccgg
     1081 cacggtggtg acggagctgc tcgatccggg cgagatggtg atgctggcca agggtggcga
     1141 cggcggcttc ggcaacctgc actacaagac cagcaccaac cgggcgccgc ggcaaaagac
     1201 accgggctgg cccggcgagc agaagaagct caagctcgag ttgcgcgtgc tggctgacgt
     1261 gggtctattg ggcatgccca atgccggcaa gtccacgctg atcgccgcga tctcgaacgc
     1321 gcggcccaag atcgccgact atcccttcac cacgctgcat cccaacctgg gcgtggtgcg
     1381 tgtcggaccc gagcgcagct tcgtcgtggc cgatgtgccg gggctgatcg agggtgccgc
     1441 cgagggagcc gggctggggc atcagttcct gcggcatctg cagcgcacgc gcctcttgct
     1501 gcacatcgtc gacctcgcgc ctttcgacga gggcgtggat ccggtggctc aggcgaaatc
     1561 catcgtcgcc gagctcaaga aatacgaccc ggcgctgtat gacaagccgc gctggctggt
     1621 gctcaacaag ctcgacatgg tgccggctga ggagcgcgcc gctcgcgtga aggacttcgt
     1681 caagcgcttc aagtggaagg gcccggtatt cgagatctcg gccctgacgc gcgaaggctg
     1741 cgagccgctg atccatgcga tctggcagca cgtggccacc tatcagacgc aggtggttga
     1801 gcccgacgtg cgcttcgatg cgccgccgcc cgtcgaagtg ccgaccgagc ccggcttcct
     1861 gcccatgccg gatgacccgc gattccgctg attgactcct ctccgcgtgc cgtatgagtg
     1921 acgttctcaa gagcgcccgc cgcatcgtcg tcaaggtggg ttccagcctc gtgaccaacg
     1981 agggccgcgg cctggacgcc gaggcgatcg gcaactggtg ccgccaactg gcggcgctgg
     2041 cgtcgcaggg ccgcgaggtc gtgatggtgt ccagcggagc tgtcgccgaa ggcatgaagc
     2101 gcctgggctg ggccaagcgg cccaaggaag tgaacgagct gcaagcggcc gctgcggtgg
     2161 gccagatggg cctggcgcag atctacgaga ccaagctgcg cgagcatggg ctgggcagcg
     2221 cccaggtgct gctcacacac gccgacctgg ccgaccgcga gcgctatctc aatgcccgct
     2281 cgacgctgct gacgctgctg tcgctcaagg tcatcccggt gatcaacgag aacgacacgg
     2341 tcgtcaacga cgagatcaag ttcggcgaca acgacacgct gggcgcgctg gtggccaatc
     2401 ttgtcgaggc cgacgcgctg gtgatcctca ccgaccagaa gggcctgtat tcggccgacc
     2461 cgcgcaagga tcccaaggcg cgcttcatcg atgtcgcgca ggccggcgat ccggcgctgg
     2521 aacagatggc cggcggcgca gggtcgagca tcggccgcgg cggcatgatc accaaggtgc
     2581 tggccgctaa gcgcgccgcc ggcagcggcg ccagcacggt ggtggcctgg ggccgtgaaa
     2641 gcgacgtgct ggtgcggctg gccgcgggcg aagcgatggg cacgctcttc atcgccggca
     2701 ccgccaagca ggcggcgcgc aagcaatgga tggccgatca cctgcagctg cgcggtaccc
     2761 tggtcatcga cgacggggcg gcggtcaagt tgcgcgacga aggcaagagc ctgctgccca
     2821 tcggcgtcgt cgaggtcgcg ggcgagttcc accgcgggga cgtggtagcg gtacgaaacg
     2881 ctgcgggcct ggaaatcgca cgcggcctgg ccaactattc gagcgccgag gcgcgcctca
     2941 tcgcgcgcaa gccatccagc gagttcgagc gtctgctcgg ctacaccgcc gaacccgaaa
     3001 tgattcatag agacaatctg gtgctcgcgt gagcgagcac cagattgcgg cgcaggctaa
     3061 ctttgcgtca gcaaagttag gcgtagcggg ccgtcagtgg ggctcgccgt cgcctgggtg
     3121 cggctcggcc ggctggctgt cgaggccggc ctcggtttcg cgccgatgcg gccgcatgcc
     3181 cgagcgtaaa tagcgattgt ggtgattggt gcgcggcagg aagcgggcca gttcggtcag
     3241 cgccatctga tagacatcgc gcttgaattc gatcaccacg tccagtggca cccaatactc
     3301 ggtccaccgc caggcgtcga attccggatg gtcggtggcg cgcaaattca tgtcgttgtc
     3361 gcggccggtc aactgcagca ggaaccagat ctgcttctgg cccctgtagt ggccgcgcgc
     3421 ttcgcgccgg atgtagtggt cgggcacctc gtagcgcagc cagtcgcgcg tgcgcgcgat
     3481 gatgcgcacg tgctccggca gcaagccgac ttcttcttgc agctcgcgca acatcgcctg
     3541 ctcgggcgtt tcaccatact tgatgccgcc ctgcgggaat tgccaggaat gggtgcgtat
     3601 gcgtttgccc cagaacacct ggttccgctg gttgagcagg ataatcccga cgttgggcct
     3661 gaagccgtca cggtcgagca taattacccc acaatttcaa tactgaaatc attattgcac
     3721 cgggccgcgc ggtttcaacc ccgcgactaa ccccaagccg cccggcgctc ccacgctcgc
     3781 atttccgctg ccttgcggcg caaccgtgtc catgaaagcc acgcagttct tcatttccac
     3841 tctcaaggaa gctcccgccg acgccgagat cgtgagccac aagctgatga tgcgcgccgg
     3901 cttcatcaag cgcctgggcg caggcatcta cagctacatg ccaatggggc tgcgtgtgat
     3961 acgcaaggtc gagggcatca tccgcgagga gatgaaccgc gccggcgccg tggagctgct
     4021 gatgcccgtc gtgcagccgg ccgagctgtg gcaggagtcg gggcgcttcg agaaatacgg
     4081 gcccgagctg ctgcgcatca aggaccgcca tgagcgcgac ttcgtgatcc agccgaccag
     4141 cgaggaggtc atcaccgaca tcgcgcgcca ggagctgcgc agctatcgcc agctgccgaa
     4201 gaacttctat cacatccaga ccaagttccg cgacgagcgc cggccccgct tcggcctgat
     4261 gcgcgggcgc gagttcacga tgaaggacgc ctattccttc gatcgcgatg tcgaaggcgc
     4321 cggcaagagc tacgaggtca tgtatgcggc ctaccgcaag atcttcgacc gcctgggcct
     4381 gcagtaccgc gcagtggcgg ccgacactgg tgccatcggt ggcgaccgct cgcatgagtt
     4441 ccaggtgatc gccgatacgg gcgaagacgc cctggtctac tgcccgacca gcgactacgc
     4501 ggccaatgtc gagctggccg aggcggtgcc gttgatcgcg caaagagccg cgccggcgca
     4561 ggccttgcac aagacgccca cgcccggcaa ggcgacctgc gaggaggtgg ccgagctgct
     4621 caagctgccg ctcgtgcaga cggtgaagtc gctggtgctg gcgaccgatg tgaagaacga
     4681 agccggcgac gtggtcagga ccgcgctgtg gctgctgctg gtgcgcggcg accacgagct
     4741 caacgaagtc aaggccggca agctgcccgg cctgaaggac ggctatcgct tcgcaaccgc
     4801 ggccgagatc gagcagcact tcggctgcaa gcccggctac cttgggccga tcgggctcaa
     4861 ggagccggtg aaggttatcg ccgatcgcac cgtcgccaac atgagcgact tcgtctgcgg
     4921 cgccaacgac gaggactacc actacaccgg cgtcaactgg ggccgcgacc tgcccgagcc
     4981 cgacctcgtg gccgacattc gcaacgcgg
//
LOCUS       DASUCY010000438         2235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_315158, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000438 DASUCY010000000
VERSION     DASUCY010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315158"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VFV25_08735"
     CDS             complement(<1..242)
                     /locus_tag="VFV25_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393451.1"
                     /translation="MDAPLLGSTAALPVSHRRRLREIWRSAGWPCQDLVEVELLAAGL
                     LERVHEASGHERLRVTDAGVQVLAATLQRNRAARDA"
     gene            complement(431..1720)
                     /locus_tag="VFV25_08740"
     CDS             complement(431..1720)
                     /locus_tag="VFV25_08740"
                     /inference="COORDINATES: protein motif:HMM:NF025702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HEY1393452.1"
                     /translation="MKLRHWVSLALALATAALLTACGSGGNGSGSVRLVNATTDEASV
                     DLYVDSNSVATGITTGAVGSYVGVSSGGHVIAVNRSNGGGTLSQSQRAINKDSNYTLV
                     AYGSNGAIGTVLMTDSQTAPATGSVLVRVFNSSADAGPLDVYLTTAAAASSLNTSLVT
                     AGNIGSGQFTQYNQISTGTYTLTVTGTGNPSDVRLSVAGLALADQSVLTLILQPSPSG
                     VLVGGLLVAQGGSTTAAPLTSARARVVASVASNTAVSGSVGSTTVANGLGSPTVGQYV
                     LIPTGSQTLTLSAGGTPVSNTGLTAVAGTDYTLLVTGTAAAPTLNIITDDNRLPTVTT
                     QAKMRLINGVASQTSGISLSANFAQVASNVMPGNASSPTLITAGTGYELDINSPQSIV
                     PLYTTPQGGVTIVGQGVYTVFLLDGLSAPKGVPFRER"
     gene            complement(1900..>2235)
                     /locus_tag="VFV25_08745"
     CDS             complement(1900..>2235)
                     /locus_tag="VFV25_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY1393453.1"
                     /translation="GRPPARAVVNSPAVLLNLACAGRGIAAFSEVLAEPQVKAGQLVR
                     VLPGWCLPAVDAWLVFPGRRLMPAKSRAFIDLMIERLGKRCAELEARAQRGPGPAALP
                     GAQKPPQEI"
BASE COUNT          336 a          740 c          802 g          357 t
ORIGIN      
        1 tgggcgtcgc gcgcggcgcg gttgcgctgc agcgtcgcgg ccagcacctg cacgccggca
       61 tcggtgacgc gcaggcgctc gtggccggaa gcttcgtgca cacgctccag caggcctgcg
      121 gccagcagct cgacctcgac cagatcctgg caaggccagc cggccgagcg ccagatctcg
      181 cgcaggcggc ggcgatggct gacgggtagg gcagcggtcg aaccgagaag aggggcgtcc
      241 acgccgctag cttagcggcg ctgcgcgttt ggcatagccg gcacaaagca aaaagccggc
      301 agcggcttat caccactgcc ggcatgtgcc ggtgaacctt ctcaagcaga cgccgcggaa
      361 ccggcttttg ccgggctgct ggcgtcgccc ccttgagggg gagcgcctca ggcgctccgg
      421 gggtgggcca tcacctctct cggaagggca cgcccttcgg agccgagagg ccgtcgagca
      481 ggaagaccgt gtagacgccc tggccgacga tggtcacgcc gccctggggc gtcgtgtaga
      541 gcggcacgat ggactgcggc gagttgatgt ccagctcata gccggtccct gccgtgatca
      601 gagtgggcga ggaggcgttg cccggcatca cattactcgc cacctgggcg aagttggccg
      661 acagcgagat gccgctggtc tggctcgcaa cgccgttgat cagacgcatc ttggcctggg
      721 tggtaacagt cggcagccgg ttgtcgtcgg tgatgatgtt cagcgtcggc gcggcggccg
      781 tgccagtcac cagcaaggtg tagtcggtgc cggccacagc agtgaggccc gtgttggaaa
      841 cgggggtgcc gccggcgctc agcgtcagcg tctggctgcc ggtcgggatc agcacgtact
      901 ggcccaccgt cggcgaaccc aggccgttgg cgaccgtcgt gctgcccaca gagccgctga
      961 ccgcagtgtt gctggccacc gatgccacga cgcgagcgcg agccgaggtc aggggcgctg
     1021 cagtggtcga accgccctgg gccaccagca ggccgcccac cagcacgccg ctggggctgg
     1081 gctgcaggat cagcgtcagc accgactggt cggccagtgc caggcccgcc accgacaggc
     1141 gcacgtcgga ggggttaccg gtgccggtga cggtcagcgt gtaggtgcca gtgctgatct
     1201 ggttgtattg cgtgaactgg cccgaaccga tattgccggc cgtgaccagc gaggtgttca
     1261 gactgctggc ggcggcggcg gtggtcaggt agacgtccag cggaccggcg tcggcggagg
     1321 aattgaacac ccgcaccagc acgctgcccg tcgccggggc ggtctgggaa tccgtcatca
     1381 gcacggtgcc gatggcgccg ttgctgccgt aggccaccag cgtgtagttg ctgtccttgt
     1441 tgatggcgcg ctggctctgg ctcagcgtgc cgccgccgtt gctgcggttg acggcgatga
     1501 cgtgcccgcc ggagctgacg cccacatagc tgccgaccgc gccggtcgtg atgccggtgg
     1561 cgacggagtt gctgtcgacg tagagatcca cggaggcctc gtccgtcgtc gcgttcacca
     1621 ggcgcaccga gcccgaaccg ttgccgccgc tgccgcaggc ggtcaatagc gccgcggtag
     1681 ctagcgcgag cgccaggctc acccaatgtc tcaacttcat tcgctttcct tgaggtctga
     1741 gccgcaccgg cgcggcagcg tcatcgacag gcgattgcac cgagcctgtg ttgccgctag
     1801 tttgccgttg tgcagcgttt ggtttcctag gggattgtcg ccgtcgcctg gccctgagtg
     1861 aacgatggtt gacgataccg accgaggcgg gcgattttgt cagatttcct ggggcggctt
     1921 ttgcgcgccc ggcagggcgg ccggccccgg cccccgttgc gccctggcct cgagctcggc
     1981 gcagcgcttc cctaagcgtt cgatcatcag atcgatgaag gcccggctct tggccggcat
     2041 cagccgccgc cccggaaaca ccagccaggc atcgacggcc ggcaggcacc agcccggcag
     2101 cacgcgcacc agctggccgg ccttgacctg cggttcggcc agcacctcgc tgaaggccgc
     2161 gatgccgcgc ccggcgcagg ccagattcag gaggactgcc ggcgagttga cgacggcacg
     2221 cgcaggcggc cggcc
//
LOCUS       DASUCY010000439         2454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_604094, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000439 DASUCY010000000
VERSION     DASUCY010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604094"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VFV25_08750"
     CDS             <1..278
                     /locus_tag="VFV25_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY1393454.1"
                     /translation="LVRFMLEAGEVVRQRAATWATPTLLMWAGGDRCVAPRGSAQFAA
                     ASSAAPPGIVSAREFAPLYHEIFNEPQKQEVFVVLRDWLAQLELPQG"
     gene            333..1370
                     /locus_tag="VFV25_08755"
     CDS             333..1370
                     /locus_tag="VFV25_08755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY1393455.1"
                     /translation="MSATPPIVTNAPLHPLTPAARVDARPSISAVVPAYNEARNLPSL
                     LDALSTQLAAMSPQWEIVVVDDGSRDETPLVMQQWAQRSGIVYLRLSRNFGKETALSA
                     GIDAAQGDVVCLLDADGQHPPAMLPEMLARWRDGVDMVYTVREDRADEGLIKRWGSHL
                     FYTMMQRMSGVDMPAHAGDFRLMDRRVADALRQLPERNRYLKGLYAWVGFRSEGVPYM
                     PAARLHGESSFSFGRLLRLAIDGLTAFTTLPLRLWSMLGFGLALVSIVYAGWLVIEHF
                     VQGNPVPGFATLAVSLMFFSGVQLISIGVLGEYVGRVFEEVKQRPRYLIATRLGQGLD
                     ASSGAATPSAQ"
     gene            1367..1756
                     /locus_tag="VFV25_08760"
     CDS             1367..1756
                     /locus_tag="VFV25_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEY1393456.1"
                     /translation="MSRTAPLRFIVVGCAAALTHWLVASFAMGPLMAWPALIANVLGF
                     SVAFWVSYAGHHRWTFRSDAPHGQAIWRFALVAIGSFLLNEAMFAALLRWTPLPPRLS
                     LALVLVAVAGLTYLLGRRWAFVRSVPA"
     gene            complement(1670..>2454)
                     /locus_tag="VFV25_08765"
     CDS             complement(1670..>2454)
                     /locus_tag="VFV25_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393457.1"
                     /translation="YPAVLAVLLLPWSLLLVAALRRRGQATVPASPFHSLWIWWAVAI
                     SLFFSAPATKLVGYILPAVPPLALLAGSWLAVPGRERWLRGGALLAALVCVGGVFGAR
                     QWDTKSLRELPPVMRAAAAADPAWRDVPMVFIEAPHFEVPLLLDAKVLPWVVKNWDDP
                     KVQQIDSWPKELFDAARFDLEQGRRVLLKPQDLLARLCTTQRAWILAPLQWKAAGDWI
                     NALPEQARERETRLLRWDAANKGPAAPEQICQAGHGDQHQGQ"
BASE COUNT          361 a          861 c          845 g          387 t
ORIGIN      
        1 agctggtgcg cttcatgctc gaagccggcg aagttgttcg ccagcgcgcg gcgacctggg
       61 ccacgcccac gctgctgatg tgggccggcg gcgaccgctg cgtggcgccg cgcggcagcg
      121 cgcagttcgc tgcggccagc agcgcagcgc cgcccggcat cgtaagcgcg cgggagttcg
      181 cgcccctcta tcacgagatc ttcaacgagc cgcagaagca agaggtgttc gtcgtgctgc
      241 gcgattggct ggcgcagttg gaactcccgc aaggatgatg gaggtcggcg cggcctcaca
      301 atgccgtctg tgccaggatc ccgaccccca aaatgtctgc cacgccgccc atcgtcacca
      361 acgcccctct ccacccgttg actccagccg cgcgggtcga tgcccggccc agcatcagcg
      421 ccgtcgtgcc ggcctacaac gaggcgcgca acctgccgtc gctgctggat gccttgagca
      481 cccagctggc cgcgatgagc ccgcaatggg aaatcgtcgt cgtcgacgac ggcagccgcg
      541 acgagacgcc gctcgtgatg cagcaatggg cgcagcgcag cggcattgtc tatctgcggc
      601 tgtcgcgcaa cttcggcaag gagacggctc tgtcggccgg catcgacgcg gcccagggcg
      661 atgtggtctg cctgctcgat gccgacggcc agcatccgcc ggccatgctg cccgagatgc
      721 tggcgcgctg gcgcgatggc gtggacatgg tctacacggt gcgcgaggac cgcgccgacg
      781 aaggcctgat caagcgctgg ggcagtcatc tcttttacac gatgatgcag cgcatgagcg
      841 gcgtggacat gccggcccat gccggcgact tccgcctgat ggaccgccgc gtggccgacg
      901 cgctgcgcca gctgcccgag cgcaaccgct atctcaaggg cctgtatgcc tgggtcggtt
      961 ttcgcagcga gggcgtgcct tatatgcccg cggcgcggct gcacggtgaa tcgagctttt
     1021 ccttcggccg cctgctgcgc ctggccatcg acggcctgac cgccttcacg acgctgccct
     1081 tgaggctgtg gagcatgctg ggcttcgggc tcgcgctggt gtccatcgtc tatgcgggct
     1141 ggctggtgat cgagcatttc gtccagggca acccggtgcc gggcttcgcg accctggcag
     1201 tgagcctgat gttcttcagc ggcgtgcagc tgatctcgat cggcgtgctg ggcgaatatg
     1261 tcggccgcgt gttcgaggag gtcaagcagc gcccgcgcta cctgatcgcg acgcggctgg
     1321 ggcaaggcct ggatgccagc agcggcgctg ccaccccctc tgcgcaatga gccgaaccgc
     1381 cccgctgcgc ttcatcgtcg tcggctgcgc agccgcgctc acgcactggc tggtggccag
     1441 cttcgcgatg gggccgctca tggcctggcc ggcgctgatc gccaatgtgc tgggctttag
     1501 cgtcgccttc tgggtcagct atgccggcca tcaccgctgg accttccgca gcgatgcacc
     1561 gcacggccag gccatctggc gcttcgccct ggtggcgatc ggcagcttcc tgctcaacga
     1621 ggcgatgttc gcggcgctac tgcgctggac gccgctgccg ccgcggctct cactggccct
     1681 ggtgctggtc gccgtggccg gcctgacata tctgctcggg cgccgctggg cctttgttcg
     1741 cagcgtccca gcgtagcagg cgcgtctcgc gctcgcgcgc ctgctcgggc agggcgttga
     1801 tccaatcgcc ggccgccttc cactgcagcg gcgccaggat ccacgcacgc tgcgtagtgc
     1861 aaagccgggc cagcaggtcc tgcggcttca gcagcacgcg ccggccttgt tccagatcga
     1921 agcgggctgc gtcgaacagc tccttgggcc agctgtcgat ctgctgcacc ttggggtcgt
     1981 cccagttctt cacgacccag ggcaggacct tagcgtcgag cagcagcggc acctcgaagt
     2041 gcggcgcctc gatgaagacc atgggcacgt cgcgccaggc cgggtcggcc gccgcggcag
     2101 cccgcatcac gggcggcagc tcgcgcagcg acttggtatc ccattgccgc gcaccgaaca
     2161 cgccgccgac gcagaccagc gcggccagca gcgcgccacc acgcagccag cgctcgcgcc
     2221 ctggcaccgc gagccaggaa cccgcgagca gggccagagg cggcacggcc ggcaggatgt
     2281 agccgaccag cttggtggcc ggcgcggaga agaagaggct gatcgcgacc gcccaccaga
     2341 tccacaggct gtggaaaggg gaggccggca ccgtggcctg gccgcgccgg cgcaaggcgg
     2401 cgaccagcag caggctccag ggcaggagca gcaccgccag cacggccggg taga
//
LOCUS       DASUCY010000440         5420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_829249, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000440 DASUCY010000000
VERSION     DASUCY010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 5420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5420
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829249"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /gene="sbcB"
                     /locus_tag="VFV25_08770"
     CDS             <1..626
                     /gene="sbcB"
                     /locus_tag="VFV25_08770"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904809.1"
                     /note="Exonucleolytic cleavage in the 3'- to 5'-direction
                     to yield nucleoside 5'-phosphates; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HEY1393458.1"
                     /translation="IRERLFSKAEDLPEGIARLPIKGLHINKSPVVIGNLKTLRPEMA
                     QRWGIDVAQALRHAEMLAGRAALPRSLWEEVYARPKAEGETDVDEDLYGGFVGNDDRR
                     TLTRLRGMSAEQLAAKRLQFEDGRLDELLFRYRARNFAESLTDEERERWRAHCAARLH
                     EGQGGALALSAFFERIDALGEQSGDDERSQEILGALYDYAEAIAPEQ"
     gene            761..1699
                     /locus_tag="VFV25_08775"
     CDS             761..1699
                     /locus_tag="VFV25_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HEY1393459.1"
                     /translation="MDSTDGGPWTIAVMGAGAVGCYYGGMLARAGHAVTLIARPEHVR
                     AIERDGLFLDTTGFQAHVRLAASTSPEAVRGAKLVLVCVKSGDTERAAEALKPYLDPD
                     AVVLSLQNGVDNAERLRECLPQVVAPAVVYVATEMAGPGHVKHHGRGELVIAPWARAD
                     ERVASPEAVAAVFQAAGVPVQLTPDAVAALWSKLVLNCAYNALSAITQLPYGRVVAGE
                     GVERVMRDLVDECLAVAHASGIRLDAAATWAAVRGLAQTMPAQLSSTAQDLARGRRSE
                     IEHLNGSIVRRGEALGIATPVNRALLVLVRLVEAAR"
     gene            1793..2467
                     /locus_tag="VFV25_08780"
     CDS             1793..2467
                     /locus_tag="VFV25_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6781 family protein"
                     /protein_id="HEY1393460.1"
                     /translation="MSKPVIDHDALISMFENATARQAAQLRKAVSDATLAALQGRELS
                     LKNIRQVLSGVGGAVSAGAAKNTAANIDVEALLGKAAAGMDDALLKAVEANQVALQRF
                     VAQGSDLREKHLKKALGDLEKFEDTLFDAVKKSAGGAGAQLAGPWEQVLEKMQAGGTL
                     SGAKASAAAEQLAAQMQAAVRDSRSAGLRAAQALADSYAAMVSGVLIGMSDALQQQGK
                     AKTAKS"
     gene            2693..3760
                     /locus_tag="VFV25_08785"
     CDS             2693..3760
                     /locus_tag="VFV25_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ionic transporter y4hA"
                     /protein_id="HEY1393461.1"
                     /translation="MPRWVMLVPAIAAGMLAVALALPVGALLGLACGVALIGAVVAAV
                     HHAEVVANRLGEPFGTLVLAVAVTVIEVALILSMMLAGGPDKLTLPRDTIYSAVMIIC
                     NGVVGLCLLVGGLRHREQSFRIEGANSGFAALIALSSLSLVLPSFTTSSAGPTYTAPQ
                     LVFAAAASLVLWAIFVFVQTVRHRDYFLPASDASDENAHAAAPSNALAWASFGLLLVS
                     LVSVVGLAKVLSPSIEAAVVAAGAPKAVVGIAIAMLVLAPETWAAVRAARANRFQTSM
                     NLAIGSALASIGLTIPVVAMASIWLGMPLALGLEPKDLVMLGLSFLVASVTLGSGRTN
                     MMQGAVHLVIFAAFLFLVLVP"
     gene            complement(3788..4159)
                     /locus_tag="VFV25_08790"
     CDS             complement(3788..4159)
                     /locus_tag="VFV25_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393462.1"
                     /translation="MKKLFTLVLIVSALNAGTAWAHGDETPRHGGLVQMSGELKIELV
                     AKDDAVEVYLDDHGETVPTSKLTGKLTVLSGGAKSEAKLEPAQGDKLVAKGVKLAKGD
                     KVIALVTTEDKKNSSARFVIK"
     gene            complement(4191..4934)
                     /locus_tag="VFV25_08795"
     CDS             complement(4191..4934)
                     /locus_tag="VFV25_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HEY1393463.1"
                     /translation="MKTLGKVLTSAAFAAGFLFATGAHAHGFWFAQRANALAMVFGVG
                     ADDLDMVKRLSKVTSFGAYDEDGKEVPTRLVTDGPIVTVDVDNQPAFVAAVMDYGMWS
                     KTPDGKWHNKGKDEVPGAVVSEKNYKYGVHLRRELTAPMPLLPGQTLQIVPVKTPLPV
                     KMGQPLELRVLYNGKPAPGARVLSDWVNDPDEKGLKTGKDGTVTIKVRNQGLNVVVAI
                     FDAPPEDPAKTNQVEHLASLSFVLPHEPE"
     gene            5168..>5420
                     /locus_tag="VFV25_08800"
     CDS             5168..>5420
                     /locus_tag="VFV25_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393464.1"
                     /translation="MSAQTQARIARRVTLLTLVSLLGLGACAGPAQNASSAYEQPFVA
                     HWQEHSMPLIYHGPARHGAGYTAWMVPGILPRGSYLLISR"
BASE COUNT          826 a         1817 c         1862 g          915 t
ORIGIN      
        1 cgatccgcga gcgcctgttc agcaaggccg aggacttgcc cgaggggatc gcgcggctgc
       61 cgatcaaggg cctgcacatc aacaagtcgc ccgtcgtgat cggcaacctc aagaccttgc
      121 ggcccgagat ggcgcagcgc tggggcatcg acgtggccca ggcgctgcgc cacgccgaga
      181 tgctggctgg ccgggcggcc ctgccgcgca gcctgtggga ggaggtctat gcgaggccca
      241 aggccgaggg tgagacggat gtcgacgagg acctctatgg cggcttcgtc ggcaacgacg
      301 acaggcgcac gctgacgcgc ctgcgcggca tgtccgccga gcagctcgcg gccaagcggc
      361 ttcagttcga ggatggccgc ctggacgagc tgctgttccg ctaccgcgcc cgcaacttcg
      421 ccgagagcct caccgacgag gagcgcgaac gctggcgcgc ccattgcgcg gcacgcctgc
      481 acgagggcca gggcggcgca ctggcgctgt cggccttctt cgagcgcatc gatgccttgg
      541 gcgagcaaag cggcgacgac gagcgcagcc aggagatcct cggcgcgctc tacgattacg
      601 ccgaagcgat cgcacccgaa caatgaccca ccccccacgg ccttcagcca ccccctgcaa
      661 aggggcgacg ccggcggccc ggcaaagccg gatccgcggc gtccgcttgg caatgcccgg
      721 ttcatgcatc gcggatcgct tctagggaaa agcattggaa atggacagca cagacggagg
      781 cccctggacc atcgccgtga tgggcgcggg cgccgtgggc tgctactacg gcggcatgct
      841 ggcgcgcgcc ggccatgcgg tgacgctgat cgcgaggcct gagcatgtgc gggccatcga
      901 gcgcgatggc ctcttcctcg atacgaccgg cttccaggcc catgtgcggc tcgcggcatc
      961 gacctcgccc gaggccgtgc gcggcgcgaa gctggtgctg gtgtgcgtga agtccggcga
     1021 caccgagcgc gcggccgagg cgctgaagcc ttatctcgat cccgacgcgg tcgtgctcag
     1081 cctgcagaac ggcgtcgaca acgcggagcg cctgcgcgaa tgcctgccgc aagtggtggc
     1141 gccggccgtg gtctatgtgg cgaccgagat ggccggccct gggcatgtca agcaccatgg
     1201 tcggggcgaa ctggtgatcg ccccctgggc tcgcgccgac gagcgcgtcg ccagccccga
     1261 ggccgtggcc gcggtgttcc aggccgccgg cgtgccggtg cagctgacgc ccgatgcggt
     1321 cgcggccttg tggagcaagc tggtgctcaa ctgcgcctac aacgcgctgt cggcgatcac
     1381 tcagctgccc tatggccgcg tcgtggcggg ggagggcgtc gagcgcgtga tgcgcgacct
     1441 cgtcgacgaa tgcctcgccg tggcccatgc cagcggcatc aggctcgatg ccgccgccac
     1501 ctgggccgcg gtgcggggcc tcgcgcagac catgccggcg cagctctcgt cgaccgcgca
     1561 ggacctggcg cgcgggcggc gcagcgagat cgagcatctc aacggcagca tcgtgcgccg
     1621 cggcgaggcg ctgggcatcg ccacgccggt gaaccgtgcg ctgctggtgc tggtgcggct
     1681 ggtcgaggcg gctcgctgag attcgcaccg gccgggcttg ccgagcgcgg gcggcggtgc
     1741 gaggatgcgg cgtcgcatcc cgccctacca aaccgcaaac aaggagcctt cgatgagcaa
     1801 gcctgtgatc gaccacgacg ccttgatctc catgttcgag aatgccacgg ccaggcaggc
     1861 cgcccagctg cgcaaggcgg tcagcgacgc cacgctggcc gcgctgcagg ggcgcgagct
     1921 gagcctgaag aacatccgcc aggtgctcag cggcgtgggt ggcgcggtga gcgcgggcgc
     1981 cgcgaagaac acggccgcca atatcgatgt cgaggcgctg ctcggcaagg ccgcggccgg
     2041 catggacgat gcattgctga aggccgtgga ggccaaccaa gtcgcactgc agcgcttcgt
     2101 ggcccagggc tcggacctgc gcgagaagca tctgaagaag gcgctgggcg atctcgagaa
     2161 attcgaggac acgctgttcg acgcggtgaa gaagtcggcg ggcggtgccg gtgcccagct
     2221 tgccggaccc tgggaacagg tgctggagaa gatgcaggcc ggcggcacgc tgtcgggcgc
     2281 caaggcctcg gccgccgccg agcagctcgc cgcgcagatg caggcggccg tgcgcgatag
     2341 ccgctcggcc ggcctgcgtg ccgcccaggc cctggccgac agctatgcgg cgatggtcag
     2401 cggcgtgctg atcggcatgt ccgacgcgct gcagcagcag ggcaaggcga agacggccaa
     2461 gtcctgacga cccccaaacc tgccgctgcg cgtcaggccc cccgaggggg tctagaaacc
     2521 tcgggagcgg cccttcggtt tcttgagagg cccggatcct tcgccacggc gcgcgatggc
     2581 gcgccgtttc ttttcctcga cgaggcctcc tgctgctaca ttggcgcggg ccggcggctc
     2641 gcgccgcgcc cgctcttcgc tcttcccgtt ccctcatcac aaggagtcct ggatgccccg
     2701 gtgggtcatg ctcgtccctg cgattgccgc gggcatgctt gccgtcgcgc tcgccttgcc
     2761 cgtcggcgcc ttgctgggcc tggcctgcgg cgtagccctg atcggggcgg tggttgccgc
     2821 cgtccaccac gccgaggtgg tggccaatcg cctcggcgag cccttcggca cgctggtgct
     2881 ggcggtggcc gtcacggtga tcgaggtggc gctcatcctc tcgatgatgc tggccggcgg
     2941 gccggacaag ctcacgctgc cgcgcgacac gatctactcg gccgtcatga tcatctgcaa
     3001 cggcgtggtg ggcctgtgcc tgctggtcgg cggcctgcgg caccgcgagc agtccttccg
     3061 catcgagggc gcgaactcgg gcttcgcggc gctgatcgcg ctgtcctcgc tgtcgctggt
     3121 gctgcccagc ttcacgacca gctcggccgg gccaacctac acggcgccgc agctggtgtt
     3181 cgcggcggcg gcgtcgctgg tcctgtgggc gatcttcgtc ttcgtgcaga cggtgcggca
     3241 ccgcgattac ttcctgccgg cttccgacgc ctcggacgag aacgcgcatg ccgctgcgcc
     3301 gtcgaatgcg ctggcctggg cgagcttcgg gctgctgctg gtctcgctgg tgtcggtggt
     3361 ggggctggcc aaggtgctgt cgcccagcat cgaggccgcc gtggtcgcgg ccggcgcacc
     3421 caaggcagtg gtcggcatcg ccatcgcgat gctcgtgctg gcgcccgaga cctgggccgc
     3481 cgtgcgcgcc gcccgcgcca accgctttca gaccagcatg aacctcgcga tcggctcggc
     3541 gctagccagc ataggcctca ccatccccgt ggttgcgatg gcctcgatct ggctcgggat
     3601 gccgctggcg ctgggcctgg agcccaagga tctggtgatg ctgggcctga gcttcctggt
     3661 cgcctccgtc accctcggtt cgggccggac caacatgatg caaggcgccg tgcacctggt
     3721 gatcttcgcg gccttcctgt ttctggtgct ggtgccttag ggaagatctg cgcagcgcgc
     3781 cgcggcttca cttgatcacg aagcgcgcgc tgctgttctt cttgtcttcg gtcgtcacga
     3841 gggcgatcac cttgtcgcct ttcgcgagct tcacaccctt ggcgacgagc ttgtcgcctt
     3901 gagccggctc gagcttcgcc tcggacttgg cgccgccgct cagcaccgtg agcttgcccg
     3961 tgagcttgga ggtcggcacc gtctcgccgt ggtcgtcgag gtagacctcc accgcatcgt
     4021 ccttcgcgac gagctcgatc ttgagctcgc ccgacatttg cacgagcccg ccgtgccgcg
     4081 gcgtttcatc gccatgagcc catgccgtgc cggcattcag cgccgagacg atcagaacga
     4141 gagtgaacag ctttttcatc gaagcagctc ctggaaagga tgggacaaga tcattcgggc
     4201 tcatgaggga ggacaaaaga cagcgatgcc agatgctcga cctgattggt cttcgcggga
     4261 tcctcgggcg gcgcatcgaa gatcgcgacg acgacattga gaccctggtt gcgcaccttg
     4321 atcgtgacgg tgccatcctt gccggtcttc aagcccttct cgtcaggatc gttcacccaa
     4381 tcggagagca cgcgggcgcc aggtgcgggt ttgccgttgt agagcacgcg aagctccagc
     4441 ggctgcccca tcttcacggg cagcggcgtc ttcaccggca cgatctgcaa ggtctgtccg
     4501 ggcagcaggg gcatcggtgc ggtgagctcg cgccgcaggt gcactccata cttgtagttc
     4561 ttctcgctca cgacggcgcc gggcacttcg tccttgccct tgttgtgcca cttgccatcg
     4621 ggcgtcttgc tccacatgcc gtaatccatg acggcagcga cgaacgcggg ctggttatcg
     4681 acatcgaccg tgacgatcgg gccatcggtg acgaggcgcg tcggaacttc ctttccgtct
     4741 tcgtcataag cgccgaagct cgttaccttg gacaggcgtt tcaccatgtc caggtcgtcc
     4801 gcaccgacgc cgaagaccat cgcgagagcg ttggcgcgct gtgcgaacca gaaaccgtgg
     4861 gcatgcgcgc cggtcgcgaa cagaaagccg gccgcgaagg ctgccgaagt caaaaccttg
     4921 ccgagtgtct tcacatcgat gctcctgaaa gggggccgtg gaaatccaag gcgcgctcgg
     4981 aagattaact agaggcgcga gatcggttct gccgtttgct atgcggccga aaagaggaag
     5041 cttcgaaaac cggccgaaaa gagcacgctg aacaggcgct ttcgccggtg gcaccattcc
     5101 agtttcggga aatccaatcg ttgtgcgagc cgtgcatgct cgcccatctg aatcgaggag
     5161 ttgcgccatg tcagctcaga ctcaagctcg catcgctcgg cgggtgacgc tgctcaccct
     5221 ggtctcgctg ctaggcctcg gtgcctgtgc cggcccagct cagaatgcgt cgtccgcata
     5281 cgagcagccc ttcgttgcgc attggcagga gcacagcatg ccgctgatct atcacggccc
     5341 agcccgtcat ggcgctggct acacggcgtg gatggtgccc ggcatcctgc cgcgcggcag
     5401 ctacctgctg atcagccgca
//
LOCUS       DASUCY010000441         4057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1519871, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000441 DASUCY010000000
VERSION     DASUCY010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1519871"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1286)
                     /gene="mrdA"
                     /locus_tag="VFV25_08805"
     CDS             complement(<1..1286)
                     /gene="mrdA"
                     /locus_tag="VFV25_08805"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY1393465.1"
                     /translation="MIELKNIERELSRFNVRLVAAAGFVLFCFALLVARLVYLQVFRH
                     DELSTQAENNRIAVVPIVPNRGLIVDRNGVVLANNYSAYTLEIMPSKLEADLETTIDQ
                     LATVVNIEQRDRKRFKRLMEEGKGFESLPIRTKLSDEEVARFTAQRFRFPGVEIKARL
                     FRNYPYGELASHVLGYIGRINQKEKEAIDDLPEDEAANYRGTDYIGKLGLEQRYEREL
                     HGKTGVEEVETTAGGRAMRRLRATAPTPGNTLHLSIDIKLQELVEKLYGDRRGALVAI
                     DPNSGEVLAFISKPTFDPNLFVDGIDADSWKDLNESIDKPLLNRALRGTYSPGSTFKP
                     FMALAALAAGKRTPQQTIFDPGYFFFGGHKFRDDKEGGHGTVDMYRSIVQSCDTYYYM
                     LANDMGVDLIHDELQPFGFGELTGIDIDGEVRGLLPS"
     gene            complement(1354..1872)
                     /gene="mreD"
                     /locus_tag="VFV25_08810"
     CDS             complement(1354..1872)
                     /gene="mreD"
                     /locus_tag="VFV25_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEY1393466.1"
                     /translation="MMPRGADQLLLPVNPVFMWASLFVALAFNLVPLGRVPAMPDLLA
                     LTLVFWNVHQPRRVGVGAAFVFGLLMDVHDGALLGQHALAYTLLSFFAITIHRRLLWF
                     TVLSQAVQILPLFVAAHAVSLLVRVVAGGSFPGWSLVAAPVIEAMLWPLAWLVLQAPQ
                     RRAPDRDENRPL"
     gene            complement(1940..2887)
                     /gene="mreC"
                     /locus_tag="VFV25_08815"
     CDS             complement(1940..2887)
                     /gene="mreC"
                     /locus_tag="VFV25_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEY1393467.1"
                     /translation="MALGSFDRSPPPFFKQGYSSATRLVFFSALAIFLMVADTRFEIV
                     RPLRAGIATVLAPVERVLLAPVLVWSAGRDYLEGLAAARADEARARQQLAAQAERVTR
                     VEQLEQENNRLRALLGLRSRLTVQSQAAQVLYDAPDPYSRKVVIDRGRTDGINRGSPV
                     INEAGVLGQVTRVYPFTSEVTLLTDKDAAIPVLNTRSESRGVAYGDPVTASAGGGMEL
                     RFMAANADVQAGDVLQTSGLDGVYPAGLPVARVVKVNRSVDSAFARIVLTPIAQPDGA
                     RHVHVLKPIGLQMLPRPQASEAPASSAAAAAAAKKGSRR"
     gene            complement(2973..4016)
                     /locus_tag="VFV25_08820"
     CDS             complement(2973..4016)
                     /locus_tag="VFV25_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEY1393468.1"
                     /translation="MFGSFRRYFSTDLAIDLGTANTLIYVRGKGIVLDEPSVVAIRHE
                     GGPNGKKTIQAVGAEAKAMLGKVPGNIEAIRPMKDGVIADFTVTEQMLKQFIKMVHPR
                     SVLKPSPRIIICVPCGSTQVERRTIRESALGAGASDVYLIEEPMAAAIGAGLPVSEAS
                     GSMVVDIGGGTTEVGVISLGGMVYKGSVRVGGDKFDEAIINYIRRNYGMLIGEPTAEG
                     IKKSIGSAFPGSEVREMEVKGRNLSEGVPRSFTISSNEILEALTDPLNQIVSSVKNAL
                     EQTPPELGADIAERGMMLTGGGALLRDLDRLLAEETGLPVLVAEDPLTCVVRGCGMAL
                     ERMERLGAIFTSE"
BASE COUNT          642 a         1347 c         1412 g          656 t
ORIGIN      
        1 gacggcagca ggccgcgcac ctcgccgtcg atgtcgatgc cggtgagctc gccgaagccg
       61 aaaggctgca gctcgtcgtg gatcaggtcc acgcccatgt cgttggccag catgtagtag
      121 taggtgtcgc acgactgcac gatggagcgg tacatgtcca cggtgccgtg gccgccttcc
      181 ttgtcgtcgc ggaacttgtg gccgccgaag aagaaataac ccgggtcgaa gatggtctgc
      241 tgcggcgtgc gcttgcccgc ggcgagcgcg gccagggcca tgaagggctt gaaggtcgag
      301 cccggcgaat aggtgccgcg cagcgcgcgg ttgagcagcg gcttgtcgat cgactcgttg
      361 agatccttcc agctgtcggc gtcgatgccg tcgacgaaga gattggggtc gaaggtcggc
      421 ttgctgatga aggccagcac ctcgccgctg ttcggatcga tcgccaccag cgcgccgcgg
      481 cgatcgccgt agagcttctc gaccagctcc tgcagcttga tgtcgatcga caggtgcagg
      541 gtgttgcccg gcgtcggcgc cgtggcgcgc agccgccgca tggcgcggcc gcctgccgtg
      601 gtctcgacct cctcgacgcc ggtcttgccg tgcagctcgc gctcgtagcg ctgctccagg
      661 ccgagcttgc cgatgtagtc ggtgccgcgg tagttggccg cctcgtcctc gggcaggtcg
      721 tcgatcgctt ccttttcctt ctggttaatg cggccgatgt agcccagcac gtggctggcc
      781 agctcgccgt aggggtagtt gcggaacagc cgcgccttga tctccacgcc ggggaagcgg
      841 aaacgctggg ccgtgaagcg cgccacctcc tcgtcgctga gcttggtgcg gatcggcagc
      901 gactcgaagc ccttgccttc ttccatcagc cgcttgaagc gcttgcggtc gcgctgctcg
      961 atgttcacca ccgtggccag ctggtcgatc gtggtctcca ggtcggcctc gagcttggac
     1021 ggcatgatct ccagcgtgta ggccgagtag ttgttggcca gcacgacgcc attgcggtcg
     1081 acgatgagcc cgcggttggg cacgatgggc accaccgcga tgcggttgtt ctcggcctgg
     1141 gtcgacagct cgtcgtgccg gaacacctgc aggtagacca gtcgcgccac cagcagcgcg
     1201 aagcagaaca acacgaagcc ggccgcagcc accagccgca cattgaagcg gctgagctcg
     1261 cgctcgatgt tcttcagctc aatcattttt gggtagtgac tggtgacctg tgactagtga
     1321 ctggtcactg atcaccagtc actagtcact acctcacaga ggccgattct cgtcccgatc
     1381 cggcgcgcgg cgctgcggtg cctgcagcac gagccaggcc agcggccaca gcatcgcttc
     1441 gatcaccggc gccgcgacca ggctccagcc cgggaagctg ccgccggcga ccacgcgcac
     1501 cagcagcgac accgcatggg ccgccacgaa gagcggcagg atctgcactg cctgcgacag
     1561 caccgtgaac cacagcagcc ggcggtggat ggtgatggcg aagaagctga gcagcgtata
     1621 ggcgagcgca tgctggccga gcagggcgcc gtcatgcacg tccatgagga ggccgaacac
     1681 gaaggccgca ccgacgccga cgcggcgcgg ctggtgcacg ttccagaaca ccagcgtcag
     1741 cgccagcaga tcgggcatgg ccggcacgcg gccgagcggc acgaggttga aggccagcgc
     1801 gacgaacagc gaggcccaca tgaacaccgg gttgaccggc agcagcagct gatcggcgcc
     1861 gcgcggcatc atggctcaag acctttcacg gcaaaacggg gtgggcaggc atgcttggtg
     1921 ccggcgcagc gcaggaggtt catcgccggc tccctttctt ggccgctgcc gcggcggcgg
     1981 ccgaagacgc gggagcttcc gaggcttgcg gccgcggcag catctgcagg ccgatcggct
     2041 tgagcacgtg cacgtggcgg gcgccgtcgg gctgggcgat gggcgtcaac acgatgcgcg
     2101 caaaggccga gtcgacgctg cggttgacct tgacgacgcg cgcgaccggc agccccgccg
     2161 ggtagacgcc gtccagcccc gaggtctgca gcacgtcgcc ggcctgcacg tcggcattgg
     2221 cggccatgaa gcgcagctcc atgccgccgc cggcggacgc cgtgaccggg tcgccatagg
     2281 cgacgccacg gctctcgctg cgcgtgttga gcaccgggat cgcagcgtcc ttatcggtca
     2341 gcagcgtgac ctcgctggtg aaggggtaga cccgcgtcac ctggcccagc acgccggcct
     2401 cgttgatgac cggcgagccg cggttgatcc cgtcggtccg gccgcggtcg atgacgacct
     2461 tgcggctgta gggatccggc gcgtcgtaca gcacctgggc cgcctgcgac tgcacagtga
     2521 gccgcgagcg caggcccaac aaggcacgca gccggttgtt ctcctgctcg agctgctcga
     2581 cccgggtcac gcgttcggcc tgggcggcca gctgctggcg cgcccgggcc tcgtcggcgc
     2641 gcgccgcggc caggccctcg aggtagtcgc gcccggcgct ccacacgagc accggcgcca
     2701 gcagcacgcg ctccaccggc gccagcacgg tcgcgatgcc ggcgcgcagc gggcgcacga
     2761 tctcgaagcg cgtgtcggcc accatcagga agatcgccag cgccgagaag aacacgagcc
     2821 gggtcgcaga cgaatagccc tgcttaaaga agggcggcgg ggacctgtcg aagctgccga
     2881 gcgccatggt cgtcgtcggg gaagggccta gcggtaagcg acaaagccgg ccacgtggca
     2941 cacgcgccgg cttcgcctct gcatggagcg gattattccg aagtgaagat cgcgcccagg
     3001 cgctccatgc gctccagcgc catgccgcag ccgcgcacca cgcaggtcag cgggtcctcg
     3061 gccaccagca ccggcaggcc ggtttcctcg gccagcaggc ggtcgagatc gcgcaacagg
     3121 gcgccgccgc cggtcagcat catgccgcgc tcggcgatgt cggcgcccag ttcgggcggc
     3181 gtctgctcca gcgcattctt gacgctggag acgatctggt tcagcgggtc ggtcagcgcc
     3241 tcgagaatct cgttgctgct gatcgtgaag ctgcgcggca cgccctcgga gaggttgcgg
     3301 cccttgacct ccatctcgcg cacctcgctg cccgggaagg ccgagcctat gctcttcttg
     3361 atgccttcgg cggtgggctc gccaatcagc atgccgtagt tgcggcggat gtagttgatg
     3421 atggcctcgt cgaacttgtc gccgcccacg cgcaccgagc ccttgtagac catgccgccg
     3481 agtgagatca cgccgacctc ggtggtgccg ccgccaatgt cgaccaccat cgagcccgag
     3541 gcttcggaca ccggcagccc ggcgccgatc gcggccgcca tgggctcctc gatcaggtaa
     3601 acgtcgctgg cgcccgcgcc gagcgccgat tcgcggatcg tgcggcgctc gacctgcgtg
     3661 gagccgcagg gcacgcagat gatgatgcgc gggctgggct tgagcaccga gcgcgggtgc
     3721 accatcttga tgaactgctt gagcatctgc tcggtgacgg tgaagtcggc gatcacgccg
     3781 tccttcatcg ggcggatggc ctcgatgttg ccaggcacct tgcccagcat ggccttggcc
     3841 tcggcgccca cggcctggat ggtcttcttg ccgttggggc cgccttcgtg gcggatcgcg
     3901 acgaccgagg gctcgtcgag cacgatgcct ttgccgcgaa cgtagatgag ggtgttggcc
     3961 gtgccgaggt cgattgccag atcggtggag aaatagcggc ggaaggatcc gaacatgggt
     4021 gtttgctcag cggtccggca ggggcttggg taggtcc
//
LOCUS       DASUCY010000442         3391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_588290, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000442 DASUCY010000000
VERSION     DASUCY010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588290"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="VFV25_08825"
     CDS             <1..619
                     /locus_tag="VFV25_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY1393469.1"
                     /translation="EAVDGRFTPLIGLSGALGGGRSLRLAWGMHFSVICAEDMPLLDA
                     LKETPGRSQVSLTPSGGLTRSGRSGGAQKPMDASPDFGAEFAGFYRSVCRDWPRGTVP
                     ADFYRIPPSAAPALLLSGTLDPVTPPRHAARVAAALGAKARNVVVPNAGHGILGLGCT
                     RDVVYRFIDAQDEAQALKVDASCLARLPRPPMFEPWRPVLQGDQS"
     gene            616..1386
                     /locus_tag="VFV25_08830"
     CDS             616..1386
                     /locus_tag="VFV25_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY1393470.1"
                     /translation="MIQVQNVAKRFASGSGKKTRVVQAVQDLSFAAPFGAITGLLGPN
                     GAGKTTTLRMIAGLITPDAGSISVDGIDVAADSQAALARMGVLSDARGLYQRLTAREN
                     ISYYGMLQGLSREAADARAGTLAEMLEMKPLLERRVDGFSQGERMKTALARALVHDPS
                     HIILDEPTNGLDVLSTRALRETLRHLCSAAGGSKCIVFSSHIMQEVERLCDDVVVMAR
                     GRAVAEGSVAQLRERTGQADFEEAFVQLAFGEQGEQGA"
     gene            1383..2555
                     /locus_tag="VFV25_08835"
     CDS             1383..2555
                     /locus_tag="VFV25_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY1393471.1"
                     /translation="MKILRSIWIVYRKEIVDALRDRRTLTTVLLSSVLTGPLVLLLLS
                     GLVARLEERAERRVVMVQGIEHAPGLRNFIERQTYTIEAAPPDYERRLKDSKLAEPVI
                     VIPPDYAASAARGVPPVVEVVSDSSNRQAEAGSARALRLLQAYNQERAGLTLALRGVS
                     PAVLQSVDAQERDLASAQGRAAQLTGMLPFFVIMAVLYGALNAALDTTAGERERGSLE
                     PLLTNPASPASLVLGKWGAVASVGLVIAVLSVLSFLPAQWLIPSETLRAQFLFGAREA
                     ASFIVLLLPFAAALSALLMAVAIRCKSVKEAQANNALVIFGISMLPLVTLFNQTGEQP
                     WFMWVPALAQQTLMNRVLRGEALSGMQIVVPLLASAVLAALCLGFVASRLRAAAVK"
     gene            complement(2572..>3391)
                     /locus_tag="VFV25_08840"
     CDS             complement(2572..>3391)
                     /locus_tag="VFV25_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEY1393472.1"
                     /translation="AGGALVIADAMKGLLRVKGFGPRARVEVLANAVDHPSKDDPVRY
                     ADGVIVARSGMIYFTDASRRFAPGEWGGTFEASILDIVEHQSSGRLLAYDPKTRITHV
                     VMHDLCFPNGLALSADERHLFVAETGEYRIWKIAVGARALDAHALESAPGELARVLID
                     QLPGYPDNLMRGENGRLWAGLVKPRSAVADALAGIPWLRSLTLRLPRALWPVPAPYGH
                     VFAFDEDGRVLTDLQDPNGLYPETTGVTETAERLYIQSLHAPVLGWLDKRAAGL"
BASE COUNT          486 a         1148 c         1233 g          524 t
ORIGIN      
        1 cgaggcggtg gacgggcgct tcacgccact catcggcctg tccggcgcct tgggcggcgg
       61 gcgcagcctg cgactggcct ggggcatgca cttctcggtg atctgcgccg aggacatgcc
      121 gctgcttgac gcgctcaagg aaacgccggg ccgctcccaa gtttccttga ccccctcggg
      181 gggcctgacg cgcagcggca ggtctggggg cgctcagaag ccgatggatg cgagccccga
      241 cttcggcgcc gagttcgcgg gcttctatcg cagcgtctgc cgcgactggc cgcgcgggac
      301 tgtgccggcc gatttctatc gcatcccgcc tagcgccgcg ccggccttgc tgttgagcgg
      361 cacgctggat ccggtcacgc cgccgcgcca tgcagcccgc gtcgccgcgg ccctgggggc
      421 gaaggcacgc aatgtcgtgg tgccgaacgc cggccacggc atcctgggcc tgggctgcac
      481 gcgcgatgtc gtgtatcgct tcatcgacgc acaggacgag gcgcaggcgc tcaaggtcga
      541 cgcctcgtgc ctggccaggc ttccgcgccc gccgatgttc gaaccctggc ggccggtgct
      601 gcagggggac cagtcgtgat ccaggtgcag aacgtcgcca agcgctttgc cagcggcagc
      661 ggcaagaaga cgcgcgtcgt gcaggcggtg caggacctca gcttcgctgc tcccttcggc
      721 gcgatcaccg gcctgctggg ccccaacggc gcaggcaaga cgaccacgct gcgcatgatc
      781 gccggcctga tcacgccgga tgccggcagc atcagcgtcg acgggatcga cgtggcggcc
      841 gactcccagg ccgcgctggc ccgcatgggg gtgctgagcg atgcgcgcgg cctgtaccag
      901 cggctcacgg cgcgcgagaa catcagctac tacggcatgc tgcaaggcct gagccgcgag
      961 gccgccgatg cgcgcgccgg gacgctggcc gagatgctgg agatgaagcc gctgctggag
     1021 cgccgcgtcg atggcttcag ccagggcgag cgcatgaaga cggccctggc ccgcgcgctg
     1081 gtgcacgacc cctcgcacat catcctcgac gagccgacca acggcctgga cgtgctgtcc
     1141 acgcgcgcgc tgcgcgagac gctgcgccat ctgtgcagcg ctgccggcgg cagcaaatgc
     1201 atcgtcttct ctagccacat catgcaggag gtcgagcggc tctgcgacga cgtggtggtc
     1261 atggcgcgcg gccgcgcggt ggcagagggc tcggtggcgc agctgcgcga gcgcaccggc
     1321 caggccgact tcgaggaggc cttcgtgcag ctggccttcg gcgagcaagg ggagcagggc
     1381 gcatgaagat cttgagatct atctggatcg tctaccgcaa ggagatcgtc gatgcgctgc
     1441 gcgaccggcg cacgctgacc acggtgctgc tcagctcggt gctgacgggc ccgctggtgc
     1501 tgttgctgct gtccggcctc gtggcccggc tggaggagcg cgccgagagg cgcgtggtga
     1561 tggttcaggg catcgagcac gcgccgggct tgcgtaactt catcgagcgc cagacctaca
     1621 ccatcgaggc tgcgccgccc gactacgaga ggcggctgaa ggattccaag ctggccgagc
     1681 cggtgatcgt gatcccgccc gactacgcgg ccagcgcggc gcggggcgtg ccgccggtgg
     1741 tggaagtggt gagcgacagc agcaaccgcc aggccgaggc cggcagtgcc cgcgccctgc
     1801 gcctgctgca ggcctacaac caggagcgcg ccgggctcac gctggcgctg cgcggcgtga
     1861 gcccggccgt gctgcaaagc gtggatgcgc aggagcgcga tctcgccagc gcgcagggcc
     1921 gcgccgccca gctgaccggc atgctgccct tcttcgtcat catggccgtg ctctacggcg
     1981 cgctcaacgc ggcgctggac acaacggcgg gcgagcgcga gcgcggttcc ctcgagccgc
     2041 tgctgacgaa tcccgcctcg cctgcttcac tggtgcttgg caagtggggc gctgtggcga
     2101 gcgtggggct ggtgatcgcg gtgctgtcgg tgctgagctt cctgccggcg caatggctga
     2161 tcccgagcga aacgctgcgc gcgcaattcc tgttcggcgc gcgcgaggca gcgagcttca
     2221 ttgtgctgct gctgcccttt gccgcggccc tgtcggcgct attgatggcc gtggccatac
     2281 gctgcaagag cgtcaaggag gcccaggcca acaatgcgct ggtgatcttc ggcatctcga
     2341 tgctgccctt ggtcacgctg ttcaaccaga ccggcgagca gccctggttc atgtgggtgc
     2401 cggccctggc ccaacagacg ctgatgaatc gcgtgctccg tggcgaagcc ttgagcggca
     2461 tgcagatcgt ggtgccgctg ctggccagcg ccgtgctggc ggcgctgtgc ctgggcttcg
     2521 tagcgtcgcg gctgcgcgcc gcagcggtga agtgattcgc ctccgaggcg ctcagagtcc
     2581 cgcggccctc ttatccagcc agcccagcac cggcgcgtgc aggctctgga tgtagagccg
     2641 ttcggccgtc tcggtgacgc cggtggtttc cggatagagg ccgttcgggt cctgcagatc
     2701 ggtcagcacg cggccgtctt cgtcgaaggc gaagacatgg ccgtagggcg ccggtaccgg
     2761 ccacagcgcg cgcggcaggc gcagcgtgag gctgcgcagc caggggatgc cggccagcgc
     2821 gtcggcgacg gcgctacgcg gcttgacgag acccgcccac aggcggccgt tctcgccgcg
     2881 catcaggttg tcggggtagc cgggcaactg gtcgatcaga acccgggcca attcgccggg
     2941 cgcggactcc agcgcgtggg cgtccagggc gcgcgcgccg acggcgatct tccagatgcg
     3001 gtactccccg gtctcggcga cgaagagatg gcgctcgtcc gccgacaggg ccaggccgtt
     3061 ggggaagcac aggtcgtgca tcaccacgtg cgtgatgcgg gtcttggggt cataggccag
     3121 cagccgccca ctggactggt gctcgacgat gtcgaggatg ctggcctcga aggtgccgcc
     3181 ccattctccc ggcgcgaagc ggcgcgacgc gtcggtgaaa tagatcatgc cgctgcgggc
     3241 gacgatcaca ccgtcggcat agcgcaccgg atcgtccttg ctcggatggt cgaccgcgtt
     3301 ggccaggacc tcgacgcgcg cgcgcggccc gaagcccttg acgcgcagca ggcccttcat
     3361 cgcatcggcg atgacgagcg cgccgccggc g
//
LOCUS       DASUCY010000443         2546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1230789, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000443 DASUCY010000000
VERSION     DASUCY010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1230789"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1095
                     /locus_tag="VFV25_08845"
     CDS             <1..1095
                     /locus_tag="VFV25_08845"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEY1393473.1"
                     /translation="LATQLGLPGVAAQRARELAAFALLLRVPVVSLLRRESDGESALA
                     PSPLLERLLLSLRREQREAAVLPERHMPRRLALQPVVRPLPRLGRQPELLPRQLSATA
                     CEALRACPYRFHALVGLGLREADELDDAIEKRDVGTWLHAVLQRFHVERGAMNDAPAD
                     EAQLQAVADSVREEMGLLQADFLPHALWFQQLIPGYVAWLHKDEAAGAHFREGEVELK
                     AQPEELAGVGVSLRGRIDRLDGVYESGGHALRIVDYKASRPNDWRKRVKQPLEDTQLA
                     FYAALLQTARAEPPAGLHAAYLALEGDKPSLVEHAQVGASAQQLLLGLADELGRIAAG
                     EPLPALGEEPACTYCEARGLCRRDHWGDTP"
     gene            1092..>2546
                     /locus_tag="VFV25_08850"
     CDS             1092..>2546
                     /locus_tag="VFV25_08850"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEY1393474.1"
                     /translation="MSAAASYFIDRQRVESTEFYAAACDPRRSVVVEACAGAGKTWLL
                     VSRILRALLDGAQPQEILAITFTKKAAGEMRQRLNEWLQLYATAGEAQRIEALRLRGC
                     NAAEAARLAPALAQLYERVLAGGRPVEIRTFHGWFSQLMRQAPLELLAELGLQPDMAL
                     LEDFSEHRPELWRRFLGRVADDPGLHEDYRALVLQRGRGSAQKWLEAALDKRIELELA
                     DMADTLEASVPPASALWSECAGLNTPAALLRERRLNEMLWQLARSMGASGKTKQVEAA
                     HHLEAALGEGDDAAAFDGVWHALFTGNGTPRKQLGEHPELVEACEALLHIHEALDQQA
                     AHEEHLRLVRLARALLGEYASFKRERGWADMADLERCAVHLLRDATLAAWVQERLDAR
                     VRHLLIDEFQDTSSLQWQALHAWLEAYAGAGGGLSGHAPLSVFIVGDPKQSIYRFRRA
                     EPRVFAAAQDFVRQALGGSLLACDHTWRNAPEVLG"
BASE COUNT          380 a          871 c          910 g          385 t
ORIGIN      
        1 ctggccaccc agctgggcct gcccggcgtg gccgcccagc gcgcgcgcga actggctgcc
       61 tttgccttgc tgctgcgcgt gcctgtcgtc agcctgctgc gccgcgaatc ggatggcgaa
      121 tcggcgcttg cgcccagccc cttgctggag cgcctgctct tgagcctgcg ccgcgaacag
      181 cgcgaagccg ctgtgctgcc cgagcgccac atgccgcggc ggctggcctt gcagccggtg
      241 gtgcggccgt tgccgcgcct ggggcgccag cccgagctgc tgccgcgcca gctcagcgcc
      301 accgcctgcg aggccttgcg cgcctgtccc tatcgcttcc atgccttggt gggcttgggt
      361 ctgcgcgaag ccgacgagct ggacgacgcg atagaaaagc gcgatgtcgg cacctggctg
      421 cacgcggtgc tgcagcgctt ccatgtcgag cgcggcgcaa tgaatgatgc gccggccgac
      481 gaggcgcagc tgcaggccgt ggcggacagc gtccgcgaag aaatgggcct gctacaggcg
      541 gattttcttc cgcacgcgct atggtttcag cagctgatac ccggctatgt agcctggctg
      601 cacaaggacg aggctgctgg cgcgcatttc cgtgagggcg aggtcgaatt gaaggcacag
      661 cccgaagagc tggccggcgt cggcgtgagc ctgcggggac gcatcgaccg cctcgacggc
      721 gtctatgaaa gcggcggcca tgcgctacgc atcgtcgact acaaggccag caggcccaac
      781 gactggcgca agcgtgtcaa gcagccgctg gaagacactc agctcgcctt ctatgcggcc
      841 ctgctgcaga ccgcgcgtgc cgagccgccg gccggcctgc acgccgccta cctcgccctg
      901 gagggcgaca agcccagcct cgtcgaacac gcgcaagttg gcgcgagtgc ccagcaattg
      961 ctgttgggcc tggccgacga gctcggccgc atcgctgcag gcgagccctt gccggcgctc
     1021 ggcgaagagc cggcctgcac gtattgcgaa gcacgcgggc tgtgcaggcg cgaccattgg
     1081 ggcgacacac cgtgagcgct gcggcctcct atttcatcga tcgccagcgg gtcgaaagca
     1141 ctgagttcta tgccgcggcc tgcgatccgc ggcgcagcgt ggtcgtcgaa gcctgtgccg
     1201 gcgcgggcaa gacctggctt ctggtctcgc gtatcctgcg agccttgctc gacggcgcgc
     1261 agccgcagga aatcctcgcc atcaccttca cgaagaaggc ggccggcgag atgcgccagc
     1321 gcctgaacga atggctgcag ctctatgcca cggcgggcga ggcgcagcgc atcgaagcct
     1381 tgcgcctgcg cggctgcaat gccgccgagg cggcgcggct cgcgcccgcc ctggctcagc
     1441 tctacgagcg cgtgctggca ggcggacggc cagtggagat tcgcaccttc cacggctggt
     1501 tctcgcaact gatgcgccag gcgccgctgg agctgctggc cgagctgggc ctgcagcccg
     1561 acatggcgct gctggaagac ttcagcgagc accggcccga gctgtggcgg cgcttcctgg
     1621 gccgcgtggc ggacgatccc gggctgcacg aggactatcg cgcgctggtg ctgcagcgcg
     1681 ggcgcggcag cgcgcagaag tggctcgagg cggcgctgga caagcgcatc gaactcgaac
     1741 tggccgatat ggccgacacg ctggaagcga gcgtgccgcc ggcctcggcg ctgtggtccg
     1801 agtgcgccgg gctgaacacg cctgccgcct tgcttcgcga gcgccgcctg aacgagatgc
     1861 tgtggcagct ggcgcgcagc atgggcgcca gcggcaagac caagcaggtt gaggcggcgc
     1921 accatctgga agcagcgctc ggcgagggcg acgatgcggc ggcgtttgac ggtgtgtggc
     1981 acgcgctctt tacaggcaac ggcacgccgc gcaagcagct gggcgagcat ccggagctgg
     2041 ttgaggcctg cgaagccctg ctgcatatac acgaggccct ggaccagcag gccgcgcacg
     2101 aggagcacct gcgcctggtg cgcctggcgc gcgcgctgtt gggcgaatac gccagcttca
     2161 agcgcgagcg cggctgggcc gacatggccg atctggagcg ctgcgccgtg catctgctgc
     2221 gagatgcaac gctggccgct tgggtgcagg agcggctgga cgcgcgcgtg cgccatctgc
     2281 tgatcgacga attccaagac accagctccc tgcaatggca ggccctgcat gcctggctgg
     2341 aggcctatgc gggcgcgggc ggcggcctga gcggccatgc gcccttgagc gtcttcatcg
     2401 tcggcgaccc caagcagagc atctaccgct tccgtcgcgc cgagccgcgc gtcttcgccg
     2461 cggctcagga cttcgtgcgc caggccctgg gcggcagcct gctggcctgc gaccacacct
     2521 ggcgcaatgc ccccgaggtg ctgggc
//
LOCUS       DASUCY010000444         2219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1182508, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000444 DASUCY010000000
VERSION     DASUCY010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182508"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..227
                     /locus_tag="VFV25_08855"
     CDS             3..227
                     /locus_tag="VFV25_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEY1393475.1"
                     /translation="MIDLQLRGDYITLDALLKATGLADSGGTAKAMVAQGKVQVDGQQ
                     ELRKTCKLRAGQVVAVTGTRVRILAAAAAS"
     gene            513..2063
                     /locus_tag="VFV25_08860"
     CDS             513..2063
                     /locus_tag="VFV25_08860"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEY1393476.1"
                     /translation="MQDQIVTPSPPPTPSPPSSQAGWGERSTAATFPAWWAPALSSLF
                     IVVWALWAVAFNQGQFGDNIEQYNWQHSLEWGYHKHPPLPTWLLGAVIRLFGMSSYWA
                     IALAAACLLGALWFTWDIARRLCGERVATVALLLWTLHLFFSSRVQLYNHNTVMVLFM
                     AATVWCSLRAAEGRLVWWIATGACAGLALLCKYQAALPLGGLLFALAWSGHLGTRRQW
                     LGLGLALATLGLVCMPHALWVTHHHFSTLHYAAEAIEPSNLWQRLGFVLSFLVNQLRM
                     FFPLLLAVAIYSIVSKLKSARAAKDDAETMQPVPEARKWFFGLIGLPALALLVMALGG
                     GVTLRNHWGVQSFQFLSVWLAYRWSLSERVDTRRLAVIVVVVQALSLGLYVIQAHDPD
                     AQLSKRRMDTEYPAQRLADEAVAKWEAATDCPLRYVAGSAFAAGLVSVYSGQAPAVYD
                     GANSPWISETDLARHGVLYVVDARTDLPADITPVGQVSLAPGARARPAARTITLGIQL
                     PAEPCSEE"
BASE COUNT          326 a          782 c          727 g          384 t
ORIGIN      
        1 aagtgatcga tcttcaactc cgcggcgact acatcacgct cgacgccctg ctcaaggcca
       61 ccgggctcgc cgacagcggc ggcaccgcca aggccatggt ggcgcagggc aaggtgcagg
      121 tggacgggca gcaggagctg cgcaagacct gcaagctccg cgcaggccag gtcgtcgcgg
      181 tgacgggcac gcgggtacgc atcctggccg cggcggcggc atcttgagcc gcgcgttgcc
      241 ctaggccagc cggcggctcc cgcgagtcgc accatatctg acatatgcct gacgccacga
      301 ttcggcaggc tcttaactca ccgcatatta gggaaaatat ggagtttcgc ggctgccaac
      361 cgcgcgcgta ctgcgcgcaa ccctctggcg gcaatctccc cgcgtcttgg cccgttcact
      421 cctgctgcga gtgcgaggtt tcgacgtaag cggcgcagtc tgctccacaa gagccatccc
      481 gcgcacgtgc ctcagtcgac attcgcacca gtatgcagga ccaaatcgtc acccctagtc
      541 cgcctccgac cccgtcgccg ccatccagcc aggcaggctg gggcgagcgc agcaccgccg
      601 ccaccttccc cgcctggtgg gcgccagcgc tgagctcgct gttcatcgtc gtctgggcct
      661 tgtgggccgt ggccttcaat cagggccaat tcggcgacaa catcgagcag tacaactggc
      721 agcacagcct ggaatggggc tatcacaagc acccgccgct gccgacctgg ctgctcggcg
      781 cagtgatccg gctgttcggc atgtccagct actgggccat cgcgctggcg gcagcttgcc
      841 tgctgggcgc actgtggttc acctgggaca tcgcgcgccg gttgtgcggc gaacgcgtgg
      901 ccactgtggc cctgttgctg tggaccctgc atctcttctt ctcctcgcgc gtccagctct
      961 acaaccacaa cacggtgatg gtgctcttca tggcggccac ggtgtggtgc agcctgcgcg
     1021 ccgccgaggg ccgactcgtg tggtggatcg ccaccggcgc ctgcgccggg ctggccctgc
     1081 tgtgcaagta ccaggcggcg ctgccgctgg gcggcctgct gttcgcgctc gcctggagcg
     1141 gtcacctcgg cacgcggcgg caatggctgg gcctcggcct cgccctcgca acgttgggcc
     1201 tggtctgcat gccgcatgcg ctatgggtga cgcatcacca cttctcgacg ctgcattacg
     1261 cggccgaggc gatcgagcct tcgaacctct ggcagcgcct gggcttcgtg ctgagcttcc
     1321 tcgtcaacca gctgcgcatg ttcttcccgc tgctgctggc ggtggcgatc tatagcatcg
     1381 tctccaagct gaagagcgcc cgtgcggcga aggatgatgc cgaaaccatg cagcccgtgc
     1441 ccgaggcgcg caaatggttc ttcggcctca tcggcctgcc cgcgctggcc ttgctggtga
     1501 tggcattggg cggcggcgtg acgctgcgca accactgggg cgtgcagagc tttcagttcc
     1561 tcagcgtctg gctggcctat cgctggagcc tgagcgagcg cgtcgacacg cgccggctgg
     1621 ccgtgatcgt cgtcgtcgtg caggcgctca gcctggggct gtacgtgatc caggcccacg
     1681 accccgatgc ccagttgtcc aagcgccgca tggacaccga atacccggcg cagcgcctgg
     1741 ccgacgaggc ggtagcgaag tgggaagccg ccaccgattg cccgctgcgc tatgtggccg
     1801 gctcggcctt cgcggctggc ctcgtgtcgg tgtactcggg ccaggcaccg gcggtctacg
     1861 acggggcgaa ttcgccctgg attagcgaga ccgacctcgc ccgccatggc gtgctctacg
     1921 tcgtcgatgc acgcaccgac ctgccggccg acatcacacc ggtcggacag gtttcactgg
     1981 cgcccggcgc ccgcgcccgc cccgcggcgc gcaccatcac gctgggcatc cagctgccgg
     2041 ccgagccctg cagcgaggag tgaggccgca cgagccgcac gctgcggctc gtgcttgcca
     2101 gcagcgccgg atcggcggcc aggctcctgc gggctgcgca cactgcgcag gccatggcca
     2161 gggtgtacaa aggctgcgtc gcttgatgcc tgcaaagcca tgtcctttcg cccggtaca
//
LOCUS       DASUCY010000445         4982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_620564, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000445 DASUCY010000000
VERSION     DASUCY010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4982
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620564"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VFV25_08865"
     CDS             complement(<1..291)
                     /locus_tag="VFV25_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393477.1"
                     /translation="MNWLINFLLLLFLALVLAAWVMLPWPGAIVLALLLTAWLLLTRR
                     GRQALSVAGVGISTLRQRLGASAVIVVGIAGVVGVLVALLAMAEGYSETLRKT"
     gene            complement(302..1468)
                     /locus_tag="VFV25_08870"
     CDS             complement(302..1468)
                     /locus_tag="VFV25_08870"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY1393478.1"
                     /translation="MKYLHLVWASLFRRKTRTVLTLVSIVAAFLLFGLLDAVRTSFSQ
                     AGQGANGAARLQTGSKLSFIQTLPQSLQGQIERVDGVKTVTYANWFGGAYQDPHNQVF
                     SFAVAPNYLDLYPEVAVTEAERKAFAQTRTGALVGEKLAQRFNWKVGDRVPLQSTIFP
                     DRQGSKNWQFDIVGILHATDKKTGGFFDQMFLLNWKYFDDTTPYNQGQVGWYVTRVVD
                     VNQADRVLRAIDALSANSDHETRTQTEQAATASWMKQLADIGLIVGSIMGAVFFTLLL
                     LSGNTMMRAVRERTSELAVLKTIGFSDGSVLAMVLAESVLLLLLGGVIGLALATAIIP
                     TISAGSGGMLNLPSVGAGSWLLGLALMGLIGVLVGALPAWSAMRLNIVDALAGR"
     gene            complement(1487..2176)
                     /locus_tag="VFV25_08875"
     CDS             complement(1487..2176)
                     /locus_tag="VFV25_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018444367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY1393479.1"
                     /translation="MANLIEIRDLSKVYERGRQKVEVLHHIDLEVAQGDFVALMGPSG
                     SGKTTLLNLIGGLDLPSGGSITVGDQRLDKLGAAALAKWRASRVGFVFQFYNLMPMLS
                     AQRNVELPLLLTKLSAAERRKRASVALQLVGLADRAVHKPTELSGGQQQRVSIARAIV
                     SDPTLLVCDEPTGDLDRQSAEDVLGLLRALNRDHGKTIVMVTHDPKAASYANHTLHLD
                     KGTLAEQVGAL"
     gene            complement(2239..3486)
                     /locus_tag="VFV25_08880"
     CDS             complement(2239..3486)
                     /locus_tag="VFV25_08880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY1393480.1"
                     /translation="MNELRIEGRQREDEGGGPPRWLWPALGGLLAVVLVAGALWWWLA
                     GESAIPVQAATAVAPSAGGGAAAVLQATGYVTARRQATVSTQITGTLTQVLIEEGDRV
                     QKGQVIARLEDSALRAALNAAQANVQTAQAQVVQAQAQLTQAQADARRQEELAASGML
                     SKQAAEQARTAVATYAAALEARRREVEAARAQAAQAQVNFDYTVVRAPFAGVVTVKAA
                     QVGEIVSPLSAGGGFTRTGVGTIVDMDSLEVDVDVNEAYIGQVKPDMPAEAVLDAYPD
                     WRIPAHVIAIVPSADRGKATVKVRVAMEQKDARIVPDMGVRVSFLGSKPAASAAQPPK
                     GVLVPPQAVAQRDDVGAVVFVVVDGKVRQHAVKPAAQDYGAMKLLQDDAGGVKAGDRV
                     VLAPPPALREGSTVKIEGETATR"
     gene            3766..4935
                     /locus_tag="VFV25_08885"
     CDS             3766..4935
                     /locus_tag="VFV25_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY1393481.1"
                     /translation="MRATSKPSLLADEECILVLQGGGALGAYQAGVFEMLSKFHREPS
                     WVAGISIGAINAALIAGNPPGERVERLREFWELVSSSLPPLPFSAPGEAREGLNEASA
                     TQVMLFGVPGFFTPRLPPAPFQPRGTLAAISYYDTTPLKETLERLVDFDRINAPGAAR
                     LSLGAVNVRSGNFEYFDSARQRIDVRHVMASGALPPGFAPVEIDGEHYWDGGLVSNTP
                     LQYVLDQPPVPRRHCVIFQVDLFSARGAFPQTLAEASEREKDIRYSSRTRLNTTNELN
                     RQVIARAAQRLLAKLPPALRDDPDAQALARLRCDGGVDVVHLIYRSKHYESQSKDYEF
                     SRVSMQEHWDAGRADMAHTLHDPRWTQHERRHHGVHVFDLTGDQPTQQSERTTTS"
BASE COUNT          769 a         1741 c         1678 g          794 t
ORIGIN      
        1 ggtcttgcgc aaggtttcgc tgtagccctc ggccatggcc agcagcgcga ccagcacgcc
       61 caccacgccg gcgatgccta cgacgatgac cgccgaggcg cccaaccgct gacgcagcgt
      121 gctgatgccc acgccggcaa ccgacagcgc ctggcggccg cgccgcgtca gcagcagcca
      181 agccgtgagc agcagcgcca gcacgatcgc gccgggccag ggcagcatca cccacgcggc
      241 gagcaccaag gccagaaaca gcagcagtag aaagttgatc agccagttca ctcggttctc
      301 ctcagcggcc ggcgagggcg tcgacgatgt tcaggcgcat ggcgctccag gccggcagcg
      361 caccgaccag cacgccgatc aggcccatca gcgcgaggcc gagcagccag ctgccggcgc
      421 ccaccgaagg caggttcagc atgccgccgc tgccggcgct gatggtcggg atgatcgcag
      481 tcgccagtgc gaggccgatc acgccgccca gcaggagcag caacaccgac tcggccagca
      541 ccatcgcgag cacgctgcca tcggagaagc cgatcgtctt gagcaccgcc agctcgctgg
      601 tgcgctcgcg cacggcccgc atcatcgtgt tgcccgacag cagcagcagc gtgaagaaca
      661 cggcgcccat gatggagccg acgatgaggc cgatgtcggc tagctgcttc atccacgacg
      721 cggtcgcagc ctgctcggtc tgggtgcgcg tctcgtggtc ggagttggcc gacagcgcgt
      781 cgatcgcgcg gagcacccgg tcggcctggt tcacgtcgac gacgcgggtc acgtaccagc
      841 ccacctggcc ctggttgtag ggcgtggtgt cgtcgaagta tttccagttc agcaagaaca
      901 tctggtcgaa gaagccgccg gtcttcttgt cggtggcgtg caggatgccg acgatgtcga
      961 actgccagtt cttgctgccc tggcggtcgg ggaagatggt cgactgcagc ggcacccggt
     1021 cgccgacctt ccagttgaag cgctgcgcca gcttttcgcc gaccagcgcg ccggtgcgcg
     1081 tctgcgcgaa ggccttgcgc tcggcctcgg tcactgcgac ttcgggatac agatcgaggt
     1141 agttgggcgc cacggcaaag ctgaacacct ggttgtgcgg gtcctggtag gcgccaccga
     1201 accaattggc ataggtcacg gtcttcacgc cgtcgacgcg ctcgatctgc ccctgcagcg
     1261 actgcggcag cgtctggatg aaggacagct tggagccggt ctgcagccgc gccgcgccgt
     1321 tcgcgccctg gccggcctgg ctaaacgagg tgcgcaccgc gtccagcagg ccgaacagca
     1381 agaaggccgc gacgatggac accagcgtca gcacggtgcg cgtcttgcgc ctgaacagcg
     1441 aggcccagac gagatggagg tatttcatga agcctcctgt ggtgcgtcag agggcgccga
     1501 cctgctcggc cagcgtgccc ttgtccagat gcagggtgtg gttagcgtaa ctggcggcct
     1561 tggggtcgtg cgtgaccatc acgatggtct taccgtggtc gcggttcagt gcgcgcaaga
     1621 ggcccagcac gtcctcggcc gactggcggt cgaggtcgcc ggtgggctcg tcgcagacca
     1681 gcagcgtcgg gtcggagacg atggcgcgcg cgatggacac gcgctgctgc tggccaccgg
     1741 acagttcggt gggcttgtgc acggcgcggt cggccaggcc caccagctgc agtgcgaccg
     1801 aagcgcgctt gcgccgctcg gcggccgaca gtttggtcag cagcagcggc agctcgacat
     1861 tgcgctgcgc cgacagcatg ggcatcaggt tgtagaactg gaacacgaag cctaccctcg
     1921 aagcgcgcca cttcgccagc gctgccgcgc cgagcttgtc cagccgctgg tcgcccaccg
     1981 tgatgctgcc gccactcggc aggtccagcc cgccgatcag gttgagcagc gtggtcttgc
     2041 ccgagcccga gggccccatc agcgcgacga agtcgccctg tgccacctcg aggtcgatat
     2101 ggtgcaggac ctcgaccttc tgcctgccgc gttcgtagac cttggacagg tcgcggatct
     2161 cgatcagatt tgccatgcct tgtccttctt cttgagtaag cgctgttgtt cagcgctgtg
     2221 tgagtaaggc gccgttgttc agcgcgttgc cgtctcgcct tcgattttta ccgtggaacc
     2281 ctcgcgcagc gccggcggcg gtgccagcac cacacggtcg cccgccttca cgccgcccgc
     2341 atcgtcttgc agtagcttca tcgcgccgta gtcctgcgcc gcaggtttca cggcgtgctg
     2401 gcgcaccttg ccatcgacca cgacgaagac gacggcgccg acgtcgtcgc gctgcgccac
     2461 cgcctgcggc ggcaccagca cgcccttggg cggctgcgcg gcactggcgg ccggcttgga
     2521 gccgaggaag gagacacgca cgcccatgtc cggcacgatg cgtgcgtctt tctgctccat
     2581 cgcgacgcgc accttcaccg tcgccttgcc gcggtcggcg gagggcacga tggcgatcac
     2641 gtgcgcgggg atgcgccagt ccgggtaggc atcgagcacg gcctcggccg gcatgtcggg
     2701 cttcacctgg ccgatatagg cctcgttgac gtcgacgtcc acttcgagcg aatccatgtc
     2761 gacgatggtg cccacgccgg tgcgcgtgaa gccgcccccg gccgacagcg gcgagacgat
     2821 ctcgccgact tgggccgcct tcaccgtgac gacgccggca aaaggcgcgc gcacgaccgt
     2881 gtagtcgaaa ttcacctggg cctgcgcggc ctgggcacgc gccgcctcga cctcgcggcg
     2941 gcgcgcttcg agcgcggcgg cataggtggc aaccgcggtg cgcgcctgct cggccgcctg
     3001 cttcgaaagc atgccgctgg cggcgagctc ttcctgccgg cgcgcatcgg cctgcgcctg
     3061 ggtcagctgg gcctgggcct gcaccacttg ggcctgggcg gtctgcacat tggcctgggc
     3121 cgcgttcaat gcggcccgca aggcgctgtc ttcgaggcgg gcgatgacct ggcccttctg
     3181 cacgcggtcg ccttcctcga tcaggacctg ggtcagcgtg cctgtgatct gggtcgagac
     3241 ggtcgcctgc cggcgcgcgg tgacatagcc cgtggcctgc agcaccgcgg ccgcgccgcc
     3301 gccagcgctg ggtgccaccg ccgtggccgc ctgcacggga atcgcactct cgccggcgag
     3361 ccaccaccac agcgcgcctg ccaccagcac gacggccagc aagccaccca gggccggcca
     3421 cagccagcgt ggcggcccgc cgccttcgtc ttcgcgctgc ctgccttcta tgcgcaattc
     3481 gttcaacaga ttcgcgtcga tttcctgttc tcccgaagcc cccgcacagg gcgaaggccc
     3541 atatcggcgg ggcagattgt gcagggtttt cgaaggcagc gtgcggcgaa gttagggggc
     3601 tgcgattctg tcctgcgcgg gggtggccgg acaggccgtc aacggccgca taaccggggt
     3661 tctgcccggc gtcatcatcg cgccggcgcg cagcgcgtat gctcgcgcga tcacgatgtt
     3721 tccgccgcgc tcatgacttc gcctgcatcg ccgcgttccg cttccgtgcg cgcaacttcc
     3781 aagccttctc tgcttgcgga cgaggaatgc atcctcgtgc tgcagggcgg cggcgccctg
     3841 ggcgcctacc aggccggggt gttcgagatg ctgagcaagt tccaccgcga gcccagctgg
     3901 gtggcgggca tctcgatcgg cgccatcaat gccgcgctga tcgcaggcaa cccgcccggc
     3961 gagcgggtgg agcggctgcg cgagttctgg gaactggtca gttcctcgct gccgccgctt
     4021 cccttctcgg cgcctggcga ggcgcgcgag ggcctgaacg aggcgagcgc gacccaggtc
     4081 atgctgttcg gcgtgcccgg cttcttcacg ccgcgcctcc cgcccgcgcc cttccagccg
     4141 cgcggcacgc tggcggcgat cagctactac gacaccacgc cgctgaagga aacgctggag
     4201 cgcctggtcg atttcgaccg catcaatgcc ccgggcgctg cgcggctgtc gctcggcgcg
     4261 gtcaatgtgc gcagcggcaa cttcgagtat ttcgacagcg cgcggcagcg catcgacgtg
     4321 cgccatgtca tggccagcgg cgcgctgccg ccgggcttcg cgccggtgga gatcgacggc
     4381 gagcattact gggacggcgg cctggtctcg aacacgccgc tgcaatacgt gctggaccag
     4441 ccgccagtgc cgcgccgcca ctgcgtgatc ttccaggtcg acctgttctc tgcgcgcggc
     4501 gccttcccgc agacgctggc cgaggcgagc gagcgcgaga aagacatccg ctactccagc
     4561 cggacgcgcc tgaacaccac caacgaactg aaccggcagg tgatcgcgcg ggcggcgcag
     4621 cgactgctgg ccaagctgcc gccggcgttg cgcgacgacc cggacgctca ggcactggcc
     4681 cgcttgcgct gcgacggcgg cgtcgatgtg gtgcacctga tctaccgcag caagcactac
     4741 gagagccagt ccaaggacta cgagttctcg cgcgtgtcga tgcaggagca ttgggacgcc
     4801 gggcgagccg acatggccca caccctgcac gacccgcgct ggacccagca cgagcgccgg
     4861 catcacggcg tgcatgtgtt cgacctgact ggcgaccagc ccacccaaca aagcgagagg
     4921 acgacgacct catgaagatc gccgacgtgc gccgccgtgc ctttgcgatg cccttgacca
     4981 gc
//
LOCUS       DASUCY010000446         3191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_10214, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000446 DASUCY010000000
VERSION     DASUCY010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10214"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="VFV25_08890"
     CDS             complement(<1..859)
                     /locus_tag="VFV25_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEY1393482.1"
                     /translation="MPTDSVLISEVGPRDGLQSVKAVMPTAHKLAWIDALYNAGLREI
                     EVCSFVPAGRLPQMADAAAVVRHALTKPGLTVMALVPNLHGAEAALAAGVHKLTLPVS
                     ASEAHSLANVRKTRMQMLEEIRNIAALRSERAPQVKIEAGISTAFGCTIQGEVKDDDV
                     VWMAASAVEAGADESGLSDTTGMANPAQVRRLFNKVRAAIGERTGAAHMHNTRGLGLA
                     NCLAAYGVGVRTFDSSQGGLGGCPYAPGASGNVVTEDLVFMFESMGISTGVDLEKMLV
                     ARAALQAGLP"
     gene            968..1699
                     /locus_tag="VFV25_08895"
     CDS             968..1699
                     /locus_tag="VFV25_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEY1393483.1"
                     /translation="MPKKSAVVSVADEQAAPGGAAAVDRAISLLTAFRSGDMALSVTE
                     LAERTRLYKSTVSRLLASLEHGRLVRRTAEGRWALGPETMRLAAIYSASFSLEDVVLP
                     VMRELVDRTQESVAFHVRQGDQRLCLFRVDSPQLLRDHIRAGDMLPLDRGAGGRVLMA
                     YSGARGRIYDQVRRDGYLMVTGDRVPGLVGISAPVWNSGRELVGALTLTVPEQRVQPS
                     FANELRRSAARLTESLGGHASSIEP"
     gene            complement(1749..2657)
                     /locus_tag="VFV25_08900"
     CDS             complement(1749..2657)
                     /locus_tag="VFV25_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY1393484.1"
                     /translation="MDRITGVQLFIRVVETGSFSKAASELGITQPTATKAVAAMEQRL
                     GARLLHRSTRGVTPTEVGALYYDKCKLIAREIDEADNLATLLQARVGGTLRISTSVAF
                     GRRALVPLVLRYMREHPEVTIDLGFDDRYVNLVEQGVDLAIRMGRLADSTLGARYLGT
                     NPWLMVAAPSYLAQRGTPHGAADLASHACLVYSSVQGDDRWSFTTPSGDEISVPVKGP
                     MRSNNLSAVLAAARAGMGLAVLPWYVARESVADGVVQQVLADHGLPAQEVHAVFPSPK
                     LVPSKVTAFIAFLQQALEGEWWRKAL"
     gene            2798..>3191
                     /locus_tag="VFV25_08905"
     CDS             2798..>3191
                     /locus_tag="VFV25_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012839516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEY1393485.1"
                     /translation="MAKMKAAMAAVLVMEKEGVMQAFGVPGAAINPLYAQLRERGTIA
                     HVLARHVEGASHMAEGYTRAKAGNIGVCIGTSGPAGTDMITGLYSAQADSIPILCITG
                     QAPRARLHKEDFQAVDIASIARPVTKWAT"
BASE COUNT          514 a         1055 c         1107 g          515 t
ORIGIN      
        1 caggcaggcc cgcctgcaaa gcggcgcgcg cgaccagcat cttctcgaga tcgacgccgg
       61 tcgagatgcc catcgactcg aacatgaaga ccaggtcctc ggtgacgaca ttgcccgacg
      121 cgcccggcgc atatgggcaa ccgccgaggc cgccctgcga gctatcgaag gtgcgcacgc
      181 cgacgccgta ggcggcgagg caattggcca gacccagtcc gcgcgtgttg tgcatgtgcg
      241 cggcaccggt cctttccccg atcgcagcgc gcaccttgtt gaaaaggcgt cgcacctgcg
      301 ccgggttggc catcccggtc gtgtccgaca ggcccgattc gtcggcgccg gcttcgacgg
      361 cggaggcggc catccatacg acgtcgtcgt ccttgacctc gccctggatc gtgcagccga
      421 aggcggtcga aatgccggcc tcgatcttga cctgcggcgc gcgctcgctg cgtagcgcgg
      481 cgatgttgcg gatctcttcg agcatctgca tgcgtgtctt gcgcacgttg gcgagcgaat
      541 gcgcttcgct cgccgacacc ggcagggtca gcttgtgtac ccccgcggca agcgcggcct
      601 cggcaccatg caggttcggc accagcgcca tgactgtcag gcccggcttg gtcaacgcat
      661 ggcgcaccac cgcggcagcg tcggccatct gcggcagtcg cccggccgga acgaaggagc
      721 agacctcgat ctcgcgcagc ccggcgttgt aaagcgcgtc gatccaagcc agcttgtgcg
      781 ccgtaggcat cacggccttg acgctctgca gaccatcgcg cggcccgact tcgctgatga
      841 gcaccgaatc ggtgggcaat tgattgtctc ctgatgttct gtacaataga acactaatct
      901 atctaatgga tcgatcttag gcgtggacct gggacgtgtc aaattcatca ccctgaaggg
      961 atagcccatg ccgaagaaat cagccgtcgt ttccgtggcc gacgagcagg ccgccccggg
     1021 cggggccgcc gcggtggacc gggccatcag cctgctgacg gcgtttcgaa gcggcgatat
     1081 ggcgctcagc gtgacggagc tggccgagcg cacacgcctg tacaagagca cggtgtcgag
     1141 gcttttggcc tcgctggagc atggccggct cgtgcggcgc acggccgagg gccgttgggc
     1201 gctcgggccg gaaaccatgc gcctggcggc gatctattcg gcgtcgttct cgctagagga
     1261 tgtcgtgctg ccggtgatgc gcgaactggt cgaccggacg caggagagcg tcgcgtttca
     1321 cgttcggcag ggggaccagc gcctctgcct gttccgtgtc gactcgccgc agctgcttcg
     1381 cgaccacatt cgtgcgggtg acatgctgcc cctggatcgc ggcgcgggcg gccgcgtcct
     1441 gatggcgtac tcgggggccc gtggacgcat ctacgatcag gtgcggcgcg acggctattt
     1501 gatggtcacc ggtgatcgcg tgccgggtct ggtgggtatc tccgcgccgg tctggaactc
     1561 ggggcgcgag ctggtgggcg cattgacctt gaccgtaccg gagcagcggg tgcaaccctc
     1621 gtttgccaac gagctccgtc gatcggccgc gcgattgacg gagtcactcg gcggccatgc
     1681 ctcgagcatc gagccctgag gtctggcgca gcgaaagggg gacgcggcaa aggcgccgcg
     1741 gatgggtcct acaaagcctt gcgccaccac tcgccctcca gcgcctgctg caggaaggcg
     1801 atgaaggccg tgaccttgga cggcacgagc ttgggtgagg gaaacaccgc gtgcacttcc
     1861 tgggccggca ggccatggtc ggccagcacc tgctgcacca cgccgtcggc caccgactcg
     1921 cgcgccacat accagggcag caccgccagc cccatgccgg cgcgcgcggc ggcgagcacg
     1981 gccgagaggt tgttcgatcg catcggcccc ttcaccggca ccgagatctc atcgcctgag
     2041 ggcgtcgtga aactccagcg gtcgtcgccc tgcacgctgc tgtagacgag gcaggcgtgg
     2101 gaggcgaggt cggccgcgcc gtgcggcgtg ccgcgctggg ccaggtagct gggcgcggcc
     2161 accatcagcc aggggttggt gcccagatag cgcgcgccca gtgtcgaatc ggcgagccga
     2221 cccatgcgga tcgcgaggtc gacgccctgc tcgacgaggt tcacatagcg gtcgtcgaag
     2281 ccgaggtcta tcgtcacctc cgggtgctcg cgcatgtagc gcagcaccag cggcaccagg
     2341 gcgcgccgac cgaaggcgac cgaggtgctg atgcgcagcg tgccgccgac gcgggcttgc
     2401 agcagcgtcg cgaggttgtc ggcctcgtcg atctcgcgcg cgatcagctt gcacttgtcg
     2461 tagtagagcg cgccgacttc ggtgggcgtg acgccgcgcg tcgagcggtg cagcagccgc
     2521 gcccccaggc gctgctccat cgcggccacc gccttggtgg cggtcggctg ggtgatgccg
     2581 agctcgctgg ccgccttgct gaagctgccg gtctcgacga cgcggatgaa gagctgcacg
     2641 ccggtgatgc ggtccatgat gggatgcact gtagccgtga acggtccggt cattccgcca
     2701 cggaatagca gttatcccct gactgggatt cacagtcgcg gccgcaaggc cgaccatgcg
     2761 ggcgatcgga caaggcatcg gagacaagga gtacgcgatg gcgaagatga aggccgcaat
     2821 ggccgccgtg ctggtgatgg agaaggaagg cgtcatgcag gccttcggcg tgccgggcgc
     2881 cgcgatcaac ccgctgtatg cgcagctgcg cgagcgcggg acgatcgccc acgtgctagc
     2941 gcgccatgtc gaaggtgcct cgcacatggc cgagggctat acccgtgcca aggcgggcaa
     3001 catcggcgtg tgcatcggca cctcgggccc ggccggaacc gacatgatca cgggcctgta
     3061 ctcggcccag gccgattcca tccccatcct gtgcatcacc ggccaggcgc cgcgcgcccg
     3121 gctgcacaag gaggacttcc aggcggtgga catcgcctcc atcgccaggc cggtcactaa
     3181 atgggccacc a
//
LOCUS       DASUCY010000447         3331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1568064, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000447 DASUCY010000000
VERSION     DASUCY010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1568064"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VFV25_08910"
     CDS             <1..193
                     /locus_tag="VFV25_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HEY1393486.1"
                     /translation="IATCPIVRTIPSEEQIRGLIALQDTGAFVPLDSRAYRFDIVLRG
                     QRATLFENAVQSTETGAAL"
     gene            190..810
                     /gene="pcaG"
                     /locus_tag="VFV25_08915"
     CDS             190..810
                     /gene="pcaG"
                     /locus_tag="VFV25_08915"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HEY1393487.1"
                     /translation="MNALLRETASQTAGPYVHIGLAPKAAGFDIYENNFGNVLVTPAT
                     RGERIRIEGRVTDGSGTLLRDVLLEIWQANADGKYNHPGDRQPGKSIDPSFRGWGRAA
                     SDFETGLYSFETIKPGSVMGRNGRPMAPHINFWIVARGINLGLNTRMYFGDEAEANAK
                     DPVLNLIEWEVRRQTLIAQREQRGKEVVYRFDIRLQGEDETVFFDV"
     gene            803..2269
                     /locus_tag="VFV25_08920"
     CDS             803..2269
                     /locus_tag="VFV25_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEY1393488.1"
                     /translation="MSESSPVFERFLSTPQVLEIFGAPSFVQAMLDVESALAQAQAAE
                     GLIAQASADAISSQCRAGLVDVEAIVSASTQAGSLAIPLVKQLTEAVARVDADAAKQV
                     HWGSTSQDIIDSANVLLTRRALSLVDRDLSALNEALLAQAQAHADAPALARTLMQPAS
                     VVSFGFKCIAWIQPLLRTQRKLRAAAADALQLQLGGAVGTLSVMGDRGPAVARRMAER
                     LGLPPPGGAWHTQRDDWIRLGCELGVLCGALGKLARDWSLMAQAEVGELAEPSGAGRG
                     GSSAMPHKRNPVAAMVALAAAQRAPQRVAALLQCMPQEHERGLGNWQAELAEWAELFL
                     IVHGAVRALAEAAPGLQVDTARMAHNIEQLQGVVNAEAVTMRLAAVLGKPRAQALMEQ
                     LSRRAIDEGRHLRELVTQAIAQDPVLVAISQQEIASLFDPQLAAAPARALAARRLPEL
                     QAAAVELRKASQGNAGPPQVSLTPSGGGLGEAQPWGRS"
     gene            2266..3084
                     /locus_tag="VFV25_08925"
     CDS             2266..3084
                     /locus_tag="VFV25_08925"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEY1393489.1"
                     /translation="MRAKDQGLKDRSTTFSEPQLRIALQGTDAGTPVVLSHALGLDLS
                     MWDGLAAELSQEHPVLRYDHRGHGGSACPRGPYSLDDLVDDAARLIREWGRGPVVFVG
                     LSMGGMVGQGLAIRHPELLQGLVLANTTALYPEEAKAAWAQRIVLVQQQGMAAIADMV
                     VERYLCSDFRAAQPSAAEALREKIIGCNPSGYAACCQAVRDVDWLSRLHQIRCPTLVI
                     AGALDVGATPAMVQAIAERIPAARLEVMAEASHLSVAESPLRFAEAVRGLLRAA"
     gene            complement(3126..>3331)
                     /locus_tag="VFV25_08930"
     CDS             complement(3126..>3331)
                     /locus_tag="VFV25_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393490.1"
                     /translation="RRTEDLGRLALLTYWEVRRWARTARQELLAEHSAEVITEHPHAS
                     RLAFLKLIDGVIAELEDIQRQLH"
BASE COUNT          509 a         1164 c         1155 g          503 t
ORIGIN      
        1 gatcgccacc tgccccatcg tgcgcaccat ccccagcgag gagcagatcc gcggcctcat
       61 cgcgctgcag gacaccggcg ccttcgtgcc gctcgacagc cgcgcctacc gcttcgacat
      121 cgtgctgcgc ggccagcgcg cgacgctgtt cgagaacgcc gtccagtcca ccgaaaccgg
      181 agccgcacta tgaacgcctt gcttcgcgaa accgcctcgc aaactgcggg cccctatgtg
      241 cacatcggcc tggcgcccaa ggcggccggc ttcgacatct acgagaacaa cttcggcaat
      301 gtgctggtga cgccggccac acgcggcgag cgcatccgca tcgagggccg ggtgaccgac
      361 ggctccggca cgctgctgcg cgacgtgctg ctcgagatct ggcaggccaa tgccgacggc
      421 aagtacaacc acccgggcga tcgccagccg ggcaagtcca tcgacccgtc cttccgcggc
      481 tggggtcgtg cggccagcga cttcgagacc ggcctctaca gcttcgagac catcaagccc
      541 ggctcggtga tgggccgcaa cggccggccc atggcgccgc atatcaactt ctggatcgtc
      601 gcgcgcggca tcaacctggg cctcaacacc cgcatgtatt tcggcgacga ggccgaggcc
      661 aacgccaagg acccggtgct caatctcatc gagtgggagg tccgccgcca gaccctcatc
      721 gcccagcgcg aacagcgcgg caaggaggtg gtctatcgtt tcgacatccg cctgcaaggc
      781 gaagacgaga ccgtgttctt cgatgtctga gtcgtcgccc gtgttcgagc gctttctgtc
      841 cacgccgcag gtgctggaga tcttcggcgc gccgtccttc gtgcaggcca tgctggacgt
      901 ggagtcggcc ctggcccagg ctcaggcggc cgaaggcctg atcgcccagg cctcggccga
      961 cgccatcagc agtcagtgcc gcgccgggct ggtggacgtc gaggcgatcg tgagcgccag
     1021 cacgcaagca ggcagcctgg cgataccgct ggtcaagcag ctcaccgagg cggtagcgcg
     1081 cgtcgatgcc gatgccgcca agcaggtgca ttggggcagc acgagccagg acatcatcga
     1141 cagcgccaac gtgctgctca cgcggcgcgc gctctcgctc gtcgatcgcg acctgtctgc
     1201 gctgaacgag gcgctcctgg ctcaagcaca ggcccatgct gacgcgccgg cgctggcgcg
     1261 cacgctgatg cagccggcct cggtcgtgag cttcggcttc aaatgcatcg cttggatcca
     1321 gcccctgctg cgcacgcagc gcaagctcag ggccgcagca gcggacgcct tgcagctgca
     1381 gctgggcggc gccgtgggta ctctgtccgt catgggcgac aggggccccg ccgtggcccg
     1441 ccgcatggcc gagcgcctgg gcctgccgcc gcccggcggc gcctggcaca cccagcgcga
     1501 cgactggatc cgcctgggct gcgagctcgg cgtgctgtgc ggcgctctag gcaagctggc
     1561 gcgcgactgg tcgctgatgg cgcaggccga ggtcggcgag ctggccgagc cctccggcgc
     1621 cggacggggc ggatccagcg ccatgccgca caagcgcaac ccggtggcgg ccatggtggc
     1681 gctcgccgcc gcacaacgcg cgccccagcg tgtcgcggcc cttttgcaat gcatgccgca
     1741 ggagcatgag cgcggtctgg gcaactggca ggccgagctg gccgaatggg ccgaactctt
     1801 cctcatcgtg cacggtgccg tccgcgccct ggcggaagcc gcacctggcc tgcaggtgga
     1861 cacagcgcgc atggcgcaca acatcgagca gctgcaaggc gtggtgaacg ccgaggcggt
     1921 gacgatgcgg ctggcggccg tgctgggcaa gcccagggcg caggcgctga tggagcagct
     1981 ctcgcgccgc gcgatcgacg aagggcggca tctgcgcgag ctcgtcacgc aggccatcgc
     2041 gcaagatccc gtgcttgtcg ccatcagcca acaagagatc gcctcgctgt tcgacccgca
     2101 gctcgctgcg gcccccgcgc gggcccttgc cgcccgccgc ctgcccgagt tgcaggccgc
     2161 tgcggtcgag ttgaggaagg cgtctcaagg aaacgccggg ccgccccaag tttccttgac
     2221 cccctcgggg ggcgggctgg gcgaggccca gccctggggg cgctcatgag agccaaggat
     2281 cagggcctga aggaccgcag caccacgttc agcgagccgc agctgcgcat cgctttgcag
     2341 ggcacggacg cgggcacgcc cgtcgtgctc agccatgcgc tgggcctgga cctttcgatg
     2401 tgggatggtc tggccgcgga gctttcgcag gagcacccgg tgctgcgtta cgaccaccgc
     2461 ggccatggcg gctcggcctg cccgcgtggc ccctattcgc tcgacgatct ggtggacgat
     2521 gcggcgcggc tgatccgcga atggggccgc ggcccagtcg tgttcgtggg gctgtcgatg
     2581 ggcggcatgg tcgggcaggg cctggcgatc cgccatcccg agctgctgca gggcctggtg
     2641 ctcgccaaca ccacagcgct ctaccccgaa gaagctaagg cggcctgggc gcagcgcatt
     2701 gtcctggtcc agcaacaagg catggcggcc atcgccgaca tggtggtcga gcgctatttg
     2761 tgcagcgact tccgcgccgc gcaaccctcg gctgccgagg ccttgcgcga gaagatcatc
     2821 ggatgcaatc cgagcggcta cgccgcctgt tgccaggccg tgcgcgatgt cgactggctg
     2881 agccggctgc atcagatccg ctgccccacg ctggtgatcg ccggcgccct ggatgtgggt
     2941 gcgacgccgg ccatggtgca ggcgattgct gagcgcatcc cggccgcccg gctcgaagtg
     3001 atggcagagg cgtcgcacct cagcgtcgcg gagtcgccac tgcgcttcgc ggaagcggtg
     3061 cgggggctgc tgcgagcggc ttagccaact ccgcacctgc gtcggcggct gcatgccggc
     3121 cggtgtcaat gcaactggcg ctggatgtcc tcgagttctg cgatcacccc gtcgatgagc
     3181 ttgaggaagg cgaggcgaga ggcgtgcgga tgctcggtga tcacttccgc tgaatgctcc
     3241 gccagcagct cctgccgggc cgtgcgcgcc cagcggcgga cttcccagta ggtcagaagc
     3301 gcgaggcgtc cgagatcttc cgttcgccgg g
//
LOCUS       DASUCY010000448         5682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_26306, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000448 DASUCY010000000
VERSION     DASUCY010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 5682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5682
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26306"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="VFV25_08935"
     CDS             complement(<1..891)
                     /locus_tag="VFV25_08935"
                     /inference="COORDINATES: protein motif:HMM:NF016894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HEY1393491.1"
                     /translation="MVGAVGAMGAARLIGLDWGSTALRAFLIDSGGKVLAERGAPLGA
                     STLSGAASFSAALAQVCGDWDMGLPMLACGMVGSRHGWLEAPYASCPAGAEQLAALAV
                     RVESGPRIVPGLLHDAAVPDVMRGEETQILGALQRHPALAEGACVILPGTHSKWATVQ
                     SGRITGFGTHMTGELYALLRRQSVLSRLMPEADAASPEAFIAGVDAARTGGDEGLGHQ
                     LFAVRTLGLMERMPVEGLADYLSGLLIGHELRAGLRSRIEGPLAIVGEPALCERYARA
                     LARFGESKPLLLANTAPDGLA"
     gene            complement(885..2033)
                     /gene="dgoD"
                     /locus_tag="VFV25_08940"
     CDS             complement(885..2033)
                     /gene="dgoD"
                     /locus_tag="VFV25_08940"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HEY1393492.1"
                     /translation="MRISKLTTYRVLPRWMFLKIETDEGIVGWGEPVIEGRARTVEAA
                     VHELGDYLVGQDPGRINDLWQLMYRAGFYRGGPILMSAIAGIDQALWDIKGKALGQPV
                     YELLGGRVRDRMKTYSWVGGDRPADVIRDIKRLREAGFDTFKMNGTEELGIVDSAAAV
                     DAAVGRIAEIRAAFGNEIEFGIDFHGRVSAPMAKVLLRALEPYRPLFVEEPVLAEQAE
                     HYPRLAESTSIPLAAGERMYSRFDFKHVFERGGISILQPDLSHAGGITECVKIAAMAE
                     AYDVALAPHCPLGPVALAACLQVDFVSYNAVLQEQSMGIHYNKDAELLDYVKNREDFR
                     LESGYIKPLPKPGLGVEIDEERVVAASAQAADWRNPVWRHADGSVAEW"
     gene            complement(2175..2564)
                     /locus_tag="VFV25_08945"
     CDS             complement(2175..2564)
                     /locus_tag="VFV25_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020200373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HEY1393493.1"
                     /translation="MARDIYSFAAALVVLLHLAFIAFAVAGAALLWRWPHVAWLHLPA
                     AAWAAYVELSGRWCPLTPLELHLRLLGGRRGYGQDFIGHYVLPLVYPSALTRETQILL
                     GLGVVLLNLILYAAWLYFRRPWQGQRA"
     gene            2788..3945
                     /locus_tag="VFV25_08950"
     CDS             2788..3945
                     /locus_tag="VFV25_08950"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY1393494.1"
                     /translation="MNLDVNTLLAVMLANVFAMAVSVPIVMGWRISTSARFVVGSAVS
                     QALAWGSFLLALPINDRLFSSLWIGLLGVSFVCMWYALRGWLGPRPGRTILLAVAGTM
                     PIGYALSFDSYAVRVGVANFGLGFIMLAVCLAAAWPAPHASRRWRGLLIATVGALAVV
                     TIGRGVLGAFFTELYPSLRTPHPLNVIGAVLNHITLTLATIALLAGWHEEAERALRRQ
                     ADIDGLTQLLNRRAWRERAEMAFALARRESGRLAVLFLDVDHFKRINDEGGHNAGDRA
                     LQLIGDLVRTCIRQGDLACRYGGEEFCVLLLGADEAAARDVDARLRAALRAREFAGAG
                     EGVRFSSGLAELQAQDHDLDALLARADGALYAAKSAGRDQLVHAGSAQLAC"
     gene            3960..4571
                     /locus_tag="VFV25_08955"
     CDS             3960..4571
                     /locus_tag="VFV25_08955"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEY1393495.1"
                     /translation="MAHPERLVYAAHACVWLVFGLTRLFVMLTLWLSNPGAAARVEGP
                     LRASEAITAPHSRLLVVLHSLAIGVVYVAVARASRGIPAWFAGQGLAGALLIASGGAL
                     MCWTLLHLRSWRLRAKLDVGHRLATSGPFRLMRHPIYMGLDLLALGTAVWIPSLLCWA
                     AFALVALGGDLRARAEERLLERSFGPAYRDYCARTRRFVPGIY"
     gene            complement(4683..5132)
                     /locus_tag="VFV25_08960"
     CDS             complement(4683..5132)
                     /locus_tag="VFV25_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEY1393496.1"
                     /translation="MQALFKIHSSFPGYRGGLKLLCASALALCASLALAQEVKLQLSG
                     DAEVPAVETTAVGSGTFKIAPDKSLTGSVTTTGIMGTMAHIHEGAAGANGRVLIPLQQ
                     DGAGKWAVPPGTKLSEEQYAAFKAGKLYVNVHSAAHPGGEIRAQLKP"
     gene            5354..>5682
                     /locus_tag="VFV25_08965"
     CDS             5354..>5682
                     /locus_tag="VFV25_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393497.1"
                     /translation="MATQPLHPEKRIHPLHGDPIRIVPLHVPEPVFTPTADALAAGPA
                     AAPLLTYRNGPLLANVEVYTIFWGPAWRQAAQSALMNQLNQFFDFVLSSPLIDQLAEY
                     DVPNYKIG"
BASE COUNT          886 a         1930 c         1924 g          942 t
ORIGIN      
        1 ggcgaggccg tccggcgcgg tattggccag cagcagcggc ttgctctcgc cgaagcgggc
       61 cagcgcccgc gcgtagcgct cgcacagcgc cggctcgccc acgatggcca ggggcccttc
      121 gatgcgcgag cgcaggccgg cacgcaattc gtggccgatc agcaatccgc tcaggtagtc
      181 ggccagaccc tcgacgggca tgcgctccat cagccccagc gtgcgcacgg caaacagctg
      241 atggccgaga ccctcgtcgc ctccggtgcg cgccgcatcc acaccggcga tgaaggcctc
      301 gggcgaagcc gcatctgcct caggcatcag gcgcgacagc accgattgcc ggcgcagcag
      361 ggcatacagc tcaccggtca tgtgcgtgcc gaagccggtg atgcgcccgc tttgcaccgt
      421 cgcccatttg gagtgcgtgc ccggcaggat cacgcaagcg ccttcggcca gcgccgggtg
      481 ccgctgcagc gcgccgagga tctgagtttc ctcgccgcgc atcacatcgg gcacggccgc
      541 atcgtgcaac agccccggca cgatgcgagg gccactttcc actcgcaccg ccagggctgc
      601 cagctgctcc gcgccggccg ggcagctcgc atacggcgcc tcgagccagc cgtgccggct
      661 gccgaccatg ccgcaggcca gcatcggcag ccccatgtcc caatcgccgc acacctgcgc
      721 cagcgctgcc gagaaggagg ctgcgccgct gagcgtcgag gccccgagcg gcgcgccgcg
      781 ctcggccagc accttgccgc cggaatcgat gagaaaggca cgcagagccg tgctgcccca
      841 gtcgagaccg atcagccggg ccgcacccat ggcgcccaca gcgcctacca ttcggccacg
      901 ctgccgtccg cgtgacgcca gacggggttg cgccaatcgg cggcctgggc gctggcggcg
      961 accacgcgct cttcgtcgat ctccacgccc aggccgggct tgggcagggg tttgatgtag
     1021 ccgctttcga ggcggaagtc ctcgcggttt ttcacgtagt ccagcagctc ggcatccttg
     1081 ttgtagtgga tgcccatgct ctgctcctgc agcacggcgt tgtacgagac gaaatccacc
     1141 tgcaggcagg ccgccagcgc caccggcccc agcgggcaat gcggtgccag cgcgacgtcg
     1201 taggcctcgg ccatggcggc gatcttcacg cactccgtga tgccgcccgc gtgcgacagg
     1261 tcgggctgca ggatgctgat gccgccgcgc tcgaagacat gcttgaaatc gaaacgcgaa
     1321 tacatgcgct cgccggccgc cagcgggatg ctggtgctct cggccaggcg cgggtaatgc
     1381 tcggcctgct cggcgagcac gggctcttcg acgaagagcg gtcgataagg ctccagcgcg
     1441 cgcagcagca ccttggccat gggcgccgaa acgcggccgt ggaagtcgat gccgaattcg
     1501 atctcgttgc cgaaggccgc gcggatttcg gcgatgcgac ccaccgccgc gtccaccgcc
     1561 gcggcgctgt cgacgatgcc gagctcctcg gtgccattca tcttgaaggt gtcgaagccc
     1621 gcttcgcgca ggcgtttgat gtcgcgaatc acatcggcag gccggtcgcc gccgacccag
     1681 gaataggtct tcatgcggtc gcgtacccgt ccgccaagca gctcgtagac cggctgcccc
     1741 agggccttgc ccttgatgtc ccacaacgcc tggtcgatcc cggcgatggc gctcatcagg
     1801 atagggccgc cgcgatagaa gccggcgcgg tacatgagct gccagaggtc gttgatgcgg
     1861 ccgggatcct ggccgacgag atagtcgccc agttcatgca cggcggcctc caccgtgcgc
     1921 gcgcgacctt cgatcacggg ctcgccccag ccgacgatgc cttcgtcggt ctcgatcttc
     1981 aggaacatcc agcgcgggag aacgcggtaa gtcgtcagct tgctgattcg cataagccca
     2041 tggctcgttc aaggaacgac caggacttta ggaggcgctg ccccgcgcga gatatgaatt
     2101 cgcgcgccgg acgtatatga gtttgggata gcgtcctcgg cgctctcaca aattgcgggc
     2161 aggaccggtc gctctcaggc ccgttgaccc tgccacgggc gccgaaagta gagccaggct
     2221 gcgtagagga tgaggttgag cagcaccaca cccaggccga gcagaatctg ggtctctcgc
     2281 gtcagtgccg aagggtagac gagcggcagc acgtagtgcc cgatgaagtc ctgcccatag
     2341 ccccgcctgc ctcccagcag gcgcaggtgg agctccagcg gggtgagcgg gcaccagcgg
     2401 cccgagagtt ccacataggc cgcccatgcg gcagcgggaa ggtgcagcca ggccacgtgg
     2461 ggccagcgcc agagcagggc ggcgcccgcc accgcgaagg cgatgaaggc gaggtgcagc
     2521 agcaccacca gcgcagctgc aaacgagtag atgtctcgag ccatggtctg cgctccgctc
     2581 cgatgggcgt cgcgctccac atgcagcggc cgtcagtatg cggcgaagcg gcgatccgcg
     2641 ctgccttttt ggagagaagc ccatgcttga ggtgctctgc tgagaggggg atggcccagc
     2701 gtgaaatagt tggcgcacgc aacacgaagc tcaaggaccg gcgcaccgag aatctccggg
     2761 cggcacgcgc cgctccctgc agcacccgtg aacctcgacg tcaacaccct cctggccgtc
     2821 atgctggcca atgtcttcgc gatggccgtt tccgtgccca tcgtgatggg ttggcgcatc
     2881 agcacctcgg ccaggttcgt ggtcgggagt gccgtcagcc aggcgctggc gtgggggagt
     2941 ttcctgctcg cgctgcccat caacgatcgg ctgttttcct cgttgtggat cgggcttctc
     3001 ggcgtgagct tcgtgtgcat gtggtatgcc ctgcgcggct ggctcgggcc gcggccgggg
     3061 cgcacgatcc tgctcgcggt cgcaggcacg atgccgatcg gctatgcgct cagcttcgac
     3121 agctatgcag tccgcgtcgg cgtggcgaac ttcggcctcg gcttcatcat gctggccgtg
     3181 tgcctggccg cggcctggcc cgcgcctcat gcaagccgcc gctggcgcgg attgctgatc
     3241 gcgacagttg gtgcactggc tgtcgtcacg atcgggcgcg gcgtgcttgg cgcctttttc
     3301 acggagctgt atcccagcct gcgcacgccg cacccgctca acgtcatcgg cgccgtgctc
     3361 aatcacataa cgctgactct ggccacgatt gccctgctcg caggctggca cgaggaagcc
     3421 gagcgtgcgc ttcgccgcca ggcggacatc gacggcttga cgcagctgct caaccggcgg
     3481 gcgtggcgcg agcgtgcaga aatggcattc gcgctggccc gccgggaaag cggacgcctc
     3541 gccgttttgt tcctggacgt cgaccacttc aagcggatca atgacgaagg cggccacaac
     3601 gccggtgatc gcgcgctgca gctcatcggg gacctcgtcc gcacctgcat acgccaaggc
     3661 gacctcgcct gccgctatgg gggcgaggag ttctgcgtgc tgctgctggg tgccgacgag
     3721 gcggcggcac gcgacgtgga cgcgcgcttg cgtgccgcac ttcgggcccg agagtttgcg
     3781 ggagcggggg aaggcgttcg cttcagcagc ggcctcgccg agctgcaggc ccaggaccat
     3841 gatctggatg cgctgctggc aagggcagat ggcgcgctgt acgcggcaaa gtcggccgga
     3901 agagaccaac tcgtgcacgc aggtagcgcg cagctcgcgt gttgacttag ggttctggca
     3961 tggcccaccc agagcgtctc gtctatgcgg cgcatgcctg cgtttggctg gtttttgggc
     4021 tgacgcggct cttcgtgatg ctgaccttgt ggctgtccaa tcccggggcg gcggcccgcg
     4081 tcgaagggcc gcttcgcgcg tccgaggcga tcactgcgcc gcattcgcgg ctgctggtgg
     4141 tcttgcactc gctggctatc ggcgtcgtgt atgtcgctgt cgctcgagcg tcccggggca
     4201 tcccggcctg gttcgccggg caaggcctgg ccggcgcatt gctcatcgcg agcggcggcg
     4261 cgctcatgtg ctggaccctg ctgcatttgc ggtcctggag gctgcgcgcc aagctcgatg
     4321 tcggccaccg gctggcgacc agcgggccct tccgcctgat gcggcatccc atctacatgg
     4381 gcctggatct gttggcgctg ggaacggcgg tctggatacc tagcctgctg tgctgggccg
     4441 cgtttgcgct cgtggccctg ggcggcgatc tgcgcgcacg cgccgaagag cgcctgctgg
     4501 agcgcagctt cgggccggcc tatcgcgact attgcgcgcg cacgcggcgc ttcgttcccg
     4561 gaatctattg atcggatggc ccccaggacc gggctacgcc cggcccgccc cccgcggggg
     4621 gcaaggaagc ttggggcggc ccggcgcttc cttgagacga gcgaagcgcc gctgctggtg
     4681 cctcagggct tgagctgggc gcggatctcg ccgccgggat gagcggcgct gtgcacgttc
     4741 acgtagagct tgccggcctt gaaggccgcg tattgctcct cgctgagctt ggttccggga
     4801 ggcacagccc atttgccggc gccatcctgc tgcagcggga tcagcacgcg gccgttggcg
     4861 cctgcagcgc cctcatggat atgcgccatc gtgcccatga ttccggtggt ggtcacgctt
     4921 ccggtgaggg acttgtcggg cgcgatcttg aacgtgccgc tgccgactgc cgtggtctcc
     4981 acggccggca cctcggcatc gccgctcagc tggagcttga cttcctgggc cagcgcaagg
     5041 ctcgcgcaca aggccagcgc gctggcgcac agcagcttca ggccaccgcg ataaccggga
     5101 aaagaagagt ggattttgaa gagagcttgc atcacgaata cctccaaggt cgacgactcg
     5161 tgaatggatc gaacaatgcc gccgcacggc ggccgaggca gggattgtgt gccgacgcgg
     5221 cggcgcgggc aaccgatcgc tgtgccagga cgtaagtcat cctcggcggg ctacccaaac
     5281 cccctcggcc gacctaggct tgtccctcca tgcggcccgc aggccgcaag gttcgccaaa
     5341 ggaggacaag atcatggcca cccagccgct tcatccggaa aagaggatcc acccgctgca
     5401 cggcgacccg atcaggatcg tgccgctgca cgtgcccgag ccggtgttca cgcccacggc
     5461 cgatgcgctc gcagccgggc cggccgccgc cccgctgctc acctaccgga acgggccgtt
     5521 gctcgcgaat gttgaggtct acaccatctt ctggggcccg gcctggcggc aggcggcgca
     5581 gagcgcgctg atgaaccagc tcaaccagtt cttcgatttc gtgctcagca gcccgctgat
     5641 cgatcagctg gccgagtacg acgtgcccaa ctacaagatc gg
//
LOCUS       DASUCY010000449         2220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_941716, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000449 DASUCY010000000
VERSION     DASUCY010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941716"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1197
                     /locus_tag="VFV25_08970"
     CDS             <1..1197
                     /locus_tag="VFV25_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEY1393498.1"
                     /translation="PEHAHEYPFCSQLAAQFDKPWVLYVAALFHDVAKGRGGDHSELG
                     AAEVRRFCREHEIAREDTQLIEFLVREHLTFSRIAQKEDLSDAEVITAFAGRAKNARW
                     LTALYLLTVADIRGTSPKVWNAWKGKLLEDLYRLTLRALGGARPNMDAEIEARKQEAR
                     HQLALHSQLPGSEEALWQTLEISYFARHDATDIAWHARALSRKVQTQVPIVKARASSL
                     GEGLQVLVYAPDQADLFARICGYFEGASFNILDAKIHTTTAGYALDTFQVISVDPVEH
                     YRDLIALVENQLAQALDSQAALPVPSRGRLSRRVKSFPMAPRVQLKPDERGQRWLLTV
                     STTDRTGLLYSIARVLARHHINLQLAKITTLGERVEDTFLIVGSALQQSKAQIEIESE
                     ILDAVS"
     gene            complement(1201..>2220)
                     /locus_tag="VFV25_08975"
     CDS             complement(1201..>2220)
                     /locus_tag="VFV25_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY1393499.1"
                     /translation="AKKGQVLVGFHERKSSYVADMTSCEILPPHVSAMLVPLRELIAA
                     MDGRDRLPQIELAIGEAAGEGGRVASVTALVLRHLEPLSEADLGRLRAFADRHGVQWW
                     LQPKGPDSVRLLDEGGPQLAYALPEFGVTMPFKPTDFTQVNHHINRVLVTRALRLLAA
                     KPDERVIDWFCGLGNFTLPIATQAREVLGVEGSESLVARSRENAACNGLAHKTRFEAR
                     NLFELEAQDLVSYGRADRWLVDPPREGAFALAKALADLAQDASLAPGYEAPRRIVYVS
                     CNPATLARDAGLLVHQAGYRCVAAGAVNMFPHTAHVESIAVFDKDDSSRPGGPGLVSV
                     SSAGV"
BASE COUNT          380 a          789 c          714 g          337 t
ORIGIN      
        1 ccggagcatg cgcacgaata tcccttctgc tcgcagctgg ccgctcagtt cgataagccc
       61 tgggtgctct atgtcgcggc gctcttccac gacgtggcca agggccgcgg cggcgaccat
      121 tcggagctcg gcgccgccga agtccggcgc ttctgccgcg agcatgaaat cgcgcgcgag
      181 gacacccagc tgatcgaatt cctggtccgc gagcacctca ccttcagccg catcgcgcag
      241 aaggaggact tgagcgacgc cgaggtcatc accgccttcg ccggacgcgc caagaacgcg
      301 cgctggctca cggccctgta tctgctgacg gtggccgaca tccgcggcac cagccccaag
      361 gtctggaacg cctggaaagg caagctgctg gaggacctct accgcctgac gctgcgtgcc
      421 ctgggcggcg cgcggcccaa catggatgca gagatcgagg cgcgcaagca ggaagcacgg
      481 catcagctgg ctctgcactc gcagttgccg ggctccgagg aagcgctgtg gcagacgctg
      541 gagatcagct atttcgcgcg ccacgacgcc accgacatcg cctggcatgc acgcgccctg
      601 tcacgcaagg tgcagaccca ggtgcccatc gtgaaggccc gcgcctcttc gctgggtgaa
      661 ggcctgcaag tgctggtcta tgcgcccgat caggccgatc tcttcgcccg catctgcggc
      721 tatttcgagg gcgccagctt caacatcctc gacgcgaaga tccacacgac gacagccggc
      781 tatgcgctgg acaccttcca ggtcatcagc gtcgatcccg tcgagcacta ccgcgacctg
      841 atcgcgctgg tcgagaacca gctggcccag gccctggaca gccaggcggc gctgcccgtg
      901 cccagccgcg gccgcctgtc gcgacgcgtc aagtccttcc cgatggcgcc gcgcgtacag
      961 ctcaagcccg acgagcgcgg ccagcgctgg ctgctcaccg tctcgacgac cgaccgcacc
     1021 ggcctgctgt actcgatcgc ccgcgtgctg gcgcggcatc acatcaacct gcagctcgcc
     1081 aagatcacga cgctgggcga gcgcgtcgag gacaccttcc tgatcgtggg cagcgccttg
     1141 cagcagagca aggcgcaaat cgagatcgag agcgagatcc tcgacgcggt cagttagccg
     1201 ctagactccc gctgacgaaa cgctgacaag acccggccca ccgggccggg aggaatcgtc
     1261 cttgtcgaac acggcaatcg actccacgtg cgccgtgtgc gggaacatgt tgaccgcgcc
     1321 ggcggcgaca cagcgatagc cggcctggtg caccagcagc cctgcatcgc gtgccagcgt
     1381 cgccggattg cagctgacgt agacgatgcg cctgggcgct tcgtagcccg gtgccagcga
     1441 ggcgtcctgc gccaggtcgg ccagggcctt ggccagcgcg aaggcgccct cgcgcggcgg
     1501 atcgaccagc catctgtcgg cacggccgta gctcaccaga tcctgggcct ccagctcgaa
     1561 gagattgcgc gcctcgaagc gggtcttgtg cgccaggccg ttgcacgccg cgttctcgcg
     1621 cgagcgggcc accagcgatt cgctgccctc cacgcccagc acctcacgcg cctgtgtcgc
     1681 gatgggcagc gtgaaattgc ccaggccgca gaaccagtcg atcacgcgtt catcgggctt
     1741 agcggcaagc agccgcagcg cgcgcgtcac cagcacgcgg ttgatgtggt ggttgacctg
     1801 ggtgaagtcg gtgggcttga agggcatggt gacgccgaat tcgggcaagg cgtaggccag
     1861 ctgcggccca ccctcgtcga gcagccgtac cgagtccggt cccttgggct gcagccacca
     1921 ttgcaccccg tgccgatctg cgaaggcgcg caagcggccc aggtcggcct cggagagcgg
     1981 ctccagatgg cgcagcacca gcgcggtgac gctggcgacc cggccgcctt cgccagcagc
     2041 ctcgcctatc gccaactcga tctgcggcag ccggtcgcgg ccatccatcg cggcgatcag
     2101 ctcgcgcagc ggaaccagca tcgcgctcac atgcggcggc aaaatctcgc agctcgtcat
     2161 gtcggccaca tagctggact tgcgctcgtg gaagccgacc agcacttggc ccttcttggc
//
LOCUS       DASUCY010000450         5319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1584002, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000450 DASUCY010000000
VERSION     DASUCY010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 5319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5319
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1584002"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="VFV25_08980"
     CDS             <1..860
                     /locus_tag="VFV25_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY1393500.1"
                     /translation="SRLMIGAEPPQLQHIQASLGEVALELRGLSLPKLSPFGTTLEDV
                     KLQVRAGEIVGIAGVSGNGQQELMAALSGEDTRAAPGSITLFGQDIARHAPHRRRRAG
                     LHFVPEERLGRGAVPTLSLSANTLLTRTEAVSRRSGWIKVGEVERLAEALIQRFNVKA
                     GGPGAAAKSLSGGNLQKFLVGREIDAAPKLLIVSQPTWGVDVGAAAQIRGELLKLRDA
                     GCALLVVSEELDELFEISDRLVVMARGRLSPSVPTSQATIEQIGEWMSGLWPGRGDAA
                     AQAKELAHA"
     gene            853..1974
                     /locus_tag="VFV25_08985"
     CDS             853..1974
                     /locus_tag="VFV25_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY1393501.1"
                     /translation="MLKLEARPQPSKRMSLASPLIALAITVVIGTILFALLGKDPLRG
                     LQVFFVEPLRNAYALSELAVKATPLILIALGLAVCFRSNIWNIGAEGQFILGAIFAGG
                     VAMQAGPETGRWIVLPILLAGMLGGMLWAGIVALLRDRFNTSEILVSLMLVYVAEMVL
                     SYLVYGPWKDPNGYNFPQTITFLEATKVPRLFAGVRINIGALMALLAVAGFWVFLFRT
                     YAGFQLQVGGLAPAAARYAGFSSRRALWIALLLSGGMAGLAGGLEATGPLGQLTPYVP
                     AGYGFAAIIVAFVGRLHPVGIIFSSLLMSMFYIGGELAQSRLGLPKALTGVFQGLLLF
                     TLLGCDTLIHYRIRWVHAARPHAPSAIPKATAIKAGENA"
     gene            1974..2894
                     /locus_tag="VFV25_08990"
     CDS             1974..2894
                     /locus_tag="VFV25_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY1393502.1"
                     /translation="MESFALLLAATLNAGTALALAGLGLLINERAGIVNLGAEGMMLV
                     AAIAGFATAVHTGNDWLAFGAGAAAGAAMAAAFGVLVIWLNTNQYASGLALSLFGAGF
                     SAFVGIGYAQDKLSERPHFQIPGLADIPFAGPALFRHHPMVYLAMALTAGLAWFLYRS
                     RAGLVLRSIGESPESAHALGYPVRRIRLLAVVVGGALCGLAGAYISVIYTPLWVEGMI
                     AGKGWIALALTTFATWRPARVLLGAYLFGGVTMLQFFLQSEGVQISSQLLTMLPYLST
                     IVVLVLISRNPTWIRINMPASLGKPFFPGS"
     gene            2945..4102
                     /locus_tag="VFV25_08995"
     CDS             2945..4102
                     /locus_tag="VFV25_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY1393503.1"
                     /translation="MSHHSKRSLLRLSAWASLAAAAALVGCGKKEEAAPPTAQAPASA
                     APAKPEPLKIGFAYVGPVGDAGWTFAHDNARKALEKEFGDRVVTSFVEKVPEGADAER
                     VFRDMVSQGDKLIFGTTFGYMEPMLKVAADAKDVKFEHATGYKQADNMRTYDSRTYEG
                     AYLAGVVAGGMTKTNTLGVVGSIPIPEVIRNINSFTLGAQSVNPKVKTKVVWVNNWFD
                     PPKETEAAQSLINGGADVLMQNTDSSAVLQTAEKAGKYAFGWDSDMTAYGPKAHLASA
                     IINWGPYYIKATHDVLDGTWKGSMHSWWGVKEGSIDLVSISDKVPADLKAKVEAAKAG
                     LKDGSLAIWKGPISSSDGKQVLKKDEVADDKFMRGIDFYVKGVEGKVPTGK"
     gene            4174..5223
                     /locus_tag="VFV25_09000"
     CDS             4174..5223
                     /locus_tag="VFV25_09000"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEY1393504.1"
                     /translation="MAMSKKLPPIPRERLPALLQTMPKAELHIHIEGSLEPELIFALA
                     QRNGVALSYPNVEALRAAYAFTDLQSFLDIYYAGASVLLKKADFHDMAWAYLSKAAAE
                     NVRHTEIFFDPQTHTARGVSMETVIKGLSSAVARARSELGLSAELILCFLRHLSEEDA
                     FATLEQALPYREYFIGVGLDSSERGHPPEKFARVFARCRELGLHLVAHAGEEGPPEYI
                     WSALDLLKVERIDHGVRSLEDPVLVQRLVRDQVPLTVCPLSNQKLRVFPKLADHNLGR
                     LLDAGLKAMVNSDDPAYFGGYLNQNFVETFDALKLDAAQAYTLARNGFEASFLDATAK
                     ARHIEALDAHFENFR"
BASE COUNT          828 a         1829 c         1759 g          903 t
ORIGIN      
        1 tgtcgcggct gatgataggc gccgagccgc cgcagctgca gcacatccag gcctcgctgg
       61 gcgaggtggc gctggagctg cgcggcctgt cgctgcccaa gctctcgccc ttcggcacca
      121 cgctcgagga cgtgaagctt caggtacgcg ccggcgagat cgtcggcatc gccggcgtct
      181 cgggcaacgg ccagcaggag ctgatggcgg ctctctcggg cgaggacacg cgcgccgccc
      241 cgggcagcat cacgctcttc ggccaggaca tcgcccgcca cgcgccgcac cggcgccgca
      301 gggcgggcct gcacttcgtg cccgaagagc gcctgggacg cggcgccgtg cccacgctgt
      361 cgctcagcgc aaacaccctg ctgacgcgca ccgaggcggt gtcgcgccgc agcggctgga
      421 tcaaggtggg cgaagtagag cgcctggccg aggcgctgat tcagcgcttc aacgtcaagg
      481 ctggcgggcc cggcgctgct gccaagagcc tgtcgggcgg caatctgcag aagttcctgg
      541 tcgggcgcga gatcgacgcg gcgccgaagc tgctcatcgt ctcgcagccg acctggggcg
      601 tggacgtggg cgccgcggcc cagatccgcg gcgagctgct caagctgcgc gatgccggct
      661 gtgcgctgct cgtggtgagc gaagagctgg acgagctgtt cgagatcagc gaccggctgg
      721 tcgtcatggc gcgcgggcgg ctctcgccca gcgtgccgac ctcccaggcc accatcgagc
      781 agatcggcga atggatgagc ggcctgtggc ccggacgcgg cgacgcggcg gcgcaggcga
      841 aggagttggc ccatgcttaa gctcgaagcg cggcctcagc cgtccaagcg catgtcgctg
      901 gcctcgccgc tgatcgcgct ggcgatcacg gtcgtcatcg gcacgatcct cttcgcgctg
      961 ctgggcaagg acccgctgcg cggcctgcag gtcttcttcg tcgagccgct gcgcaatgcc
     1021 tatgcgctgt cggagctggc cgtcaaggcc acgccgctga tcctcatcgc cctcggcctg
     1081 gccgtgtgct tccgctccaa catctggaac atcggcgccg agggccagtt catcctcggc
     1141 gcgatcttcg ccggcggcgt cgcgatgcag gcgggaccgg agaccggccg ctggatcgtg
     1201 ctgcccatcc tgctggcggg catgctgggc ggcatgttgt gggccggcat cgtcgcgctg
     1261 ctgcgcgacc gcttcaacac cagcgagatc ctggtcagcc tgatgctggt ctacgtggcc
     1321 gagatggtgc tgagctatct ggtctacggg ccctggaagg accccaatgg ctacaacttc
     1381 ccgcagacca tcacctttct cgaggcgacc aaggtgccgc gcctgttcgc cggcgttcgc
     1441 atcaacatcg gcgcgctgat ggccctgctg gcggtggccg gcttctgggt cttcctgttc
     1501 cgcacctatg cgggcttcca gctccaggtc ggcggcctgg cgccggcggc ggcgcgctat
     1561 gcgggcttct cgtcgcggcg cgcgctgtgg atcgcgctgc tactgtcggg cggcatggcg
     1621 ggcctggccg gcggtctgga agccaccggc ccgctgggcc agctcacgcc ctatgtgccg
     1681 gcgggctatg gcttcgcggc catcatcgtc gccttcgtcg gccggctgca cccggtgggc
     1741 atcatcttct cgtccctgct gatgagcatg ttctacatcg gcggcgagct ggcgcagtcg
     1801 cgcctgggcc tgcccaaagc gctgaccggc gtgttccagg gcctgctgct gttcacgctg
     1861 ctgggctgcg acacgctgat ccactaccgc atccggtggg tgcacgcggc caggccgcat
     1921 gcgccgtccg cgatacccaa agcgacggca atcaaggccg gggagaacgc ctgatggaat
     1981 cctttgccct gttgctggcc gccacgctca acgccggcac cgcgctggcg ctggcgggcc
     2041 tgggcctgct gatcaacgag cgcgccggca tcgtcaacct gggtgccgag ggcatgatgc
     2101 tggtggccgc catcgccggc ttcgcgacgg cggtgcatac cggcaacgac tggctggcct
     2161 tcggcgccgg cgccgcggcc ggcgccgcga tggcggcggc cttcggcgtg ctggtgatct
     2221 ggctcaacac caaccaatac gcatcgggcc tggcgctgag tctgttcggt gcgggcttct
     2281 cggccttcgt cggcatcggc tatgcgcagg acaagctctc cgagcggccg catttccaga
     2341 tccccggcct ggccgatatt cccttcgccg gaccggcact gttccgccat cacccgatgg
     2401 tctatctcgc gatggcgctg accgcgggcc tggcctggtt cctctaccgc tcgcgcgccg
     2461 gcttggtgct gcgctcgatc ggagaatcgc ccgagtcggc ccatgcgctg ggctatccgg
     2521 tgcggcgcat acgcctgttg gccgtggtgg tcggcggcgc gctgtgcggc ctggccggcg
     2581 cctatatctc ggtgatctac acgccgctgt gggtggaggg catgatcgcc ggcaagggct
     2641 ggatcgcgct tgcgctcacc accttcgcca cctggcggcc ggcacgcgtg ttgcttggtg
     2701 cctatctgtt cggcggcgtc acgatgttgc agttcttcct gcaaagcgag ggcgtgcaga
     2761 tctccagcca gctgctcacg atgctgcctt atttgtccac catcgtcgtg ctggtgctga
     2821 tctcgcgcaa cccgacctgg atacgcatca acatgccggc ttcgctcggc aagcctttct
     2881 ttcccggttc gtgagcgaac cgtatcatcg ccccctgcct acggtcttca gaagaggaga
     2941 acccatgtcc catcattcca agcgttccct gctcaggctc agtgcttggg cctcgctcgc
     3001 ggcagccgcc gccctggtcg gctgcggcaa gaaggaagag gccgcgccac ccacggcaca
     3061 ggcccccgcc tcggcagctc ccgccaagcc cgagccgctg aagatcggct tcgcctatgt
     3121 cgggccggtg ggcgatgccg gctggacctt cgcgcacgac aacgcgcgca aggcgctgga
     3181 gaaggaattc ggcgaccgcg ttgtcaccag cttcgtcgag aaggtgcccg agggcgccga
     3241 cgcagagcgc gtgtttcgcg acatggtgtc gcagggcgac aagctgattt tcggcaccac
     3301 cttcggctac atggagccca tgctcaaggt ggcggccgac gcgaaggacg tgaagttcga
     3361 gcatgcgacc ggctacaagc aggccgacaa catgcgtacc tacgactcgc gcacctacga
     3421 gggcgcctat ctggccggcg tcgtcgcggg cggcatgacc aagaccaaca cgctgggcgt
     3481 ggtcggctcc atcccgattc ccgaggtgat ccgcaacatc aacagcttca cgctgggtgc
     3541 gcagtcggtg aaccccaagg tcaagaccaa ggtcgtgtgg gtcaacaact ggttcgatcc
     3601 gcccaaggag accgaagcgg cccagtcgct gatcaacggc ggcgccgacg tgctgatgca
     3661 gaacaccgac tcctcggccg tgctgcagac ggcggagaag gccggcaaat acgccttcgg
     3721 ctgggacagc gacatgaccg cctacggccc caaggcccac ctcgcctcgg ccatcatcaa
     3781 ctgggggccg tactacatca aggccacgca tgacgtgctg gacggcacct ggaagggcag
     3841 catgcattcc tggtggggcg tgaaggaggg atcgatcgac ctggtctcca tctccgacaa
     3901 ggtgccggcc gatctcaagg ccaaggtcga ggccgccaag gccggcctga aggacggcag
     3961 cctcgcgatc tggaagggcc ccatctccag ctccgacggc aagcaagtgc tgaagaagga
     4021 cgaagtcgcc gacgacaagt tcatgcgcgg catagacttc tacgtgaagg gcgtggaagg
     4081 caaggtgccg actggcaagt gatcagaggc tagcagcgag gccgccgcgc ttctgcccgg
     4141 cggccttttg ttttcgtgat gaggagtcca accatggcga tgtcgaagaa gctgcccccc
     4201 atcccgcgcg agcgcctacc tgccttgctg cagaccatgc ccaaggccga gctgcacatc
     4261 cacatcgagg gctcgctgga gcccgagctc atcttcgcgc tggcccagcg caacggcgtg
     4321 gccttgagct accccaacgt cgaggccctg cgcgcggcct atgctttcac cgacctgcag
     4381 agcttcctcg acatctatta cgccggcgcc tcggtgctgc tcaagaaggc cgatttccac
     4441 gacatggcct gggcctacct gagcaaggcc gcggccgaga acgtcaggca caccgagata
     4501 ttcttcgacc cgcagaccca caccgcgcgc ggcgtgagca tggagacggt catcaagggc
     4561 ctgagcagcg cagtggcccg ggcgcgcagc gagctgggcc tgagcgccga gctgatcctg
     4621 tgcttcctgc gccacctgag cgaggaagat gccttcgcga ccctggagca ggccttgccc
     4681 tacagggagt atttcatcgg cgtcggcctc gattcgagcg agcgcggcca tccgcccgag
     4741 aagttcgcgc gcgtcttcgc acgctgccgc gagctgggcc tgcacctggt ggcccatgcc
     4801 ggcgaggaag ggccgccgga atacatctgg agcgcgctcg acttgctcaa ggtcgagcgc
     4861 atcgaccatg gcgtgcgcag cctcgaagac ccggtgctgg tgcagcgcct tgtgcgcgac
     4921 caggtgccgc tcaccgtctg cccgctgtcc aaccagaagc tccgcgtctt ccccaagctc
     4981 gccgaccaca acctcggccg tctgctcgac gcagggctca aggccatggt caactcggac
     5041 gacccggcct atttcggcgg ctatctgaac cagaacttcg tcgagacctt cgatgccctg
     5101 aagctcgacg ccgcgcaggc ctacacgctg gcgcgcaacg gcttcgaggc atcgttcctc
     5161 gatgccactg ccaaggccag gcacatcgag gcgctggacg cgcatttcga aaacttccgc
     5221 tgagcggtgc gaagcaggcc catccgcgac aatccgcggc catggaccac agcttccttt
     5281 ccgccttcat cctgctgctg ctcgtgctcg atcctttcg
//
LOCUS       DASUCY010000451         3977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1134599, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000451 DASUCY010000000
VERSION     DASUCY010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1134599"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..1531
                     /locus_tag="VFV25_09005"
     CDS             131..1531
                     /locus_tag="VFV25_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HEY1393505.1"
                     /translation="MQPSVYTARRLRVIEALRAAGGGVAVLSTAPERQRNADNDHPYR
                     HDSSFYYLTGFAEPEAWLVLTSAGRSLLFCRPRDVEREIWDGHRLGPEAAPAALGLDE
                     AHSIAALDEMMPRLLADQGAVYFPFSGYEGLGSRLAGWLDHLRSHLVPVGASAPVRQC
                     DLTGLIGEMRLIKDEHELALLRRAGTVAASAHVRAMRACAHGLRQPPPEGLREYHLEA
                     ELLYEFRRHGTSGPAYASIVAAGANACVLHYAAGDAELKSGELCLIDAGCEFGSYASD
                     VTRSFPISGRFSGPQRALYELVLAAQEAAIAHTKPGARKQDAHWAAVRCLSQGLLDLK
                     LLDRKQHGGVDDVVEKAAYRRYYMHGTGHWLGLDVHDAGEYLQLDEAPVEQPDSRGGR
                     VLKKPSRRLQPGMVVTLEPGLYVRPAADILQHYWNIGIRIEDDAVVTPEGCELITRGA
                     PVKPDEIEALMRDATA"
     gene            1528..3021
                     /locus_tag="VFV25_09010"
     CDS             1528..3021
                     /locus_tag="VFV25_09010"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEY1393506.1"
                     /translation="MTTLASGADHEFADMFELAPVSLWLEDYTELKRLFERWRGEGVS
                     DLRSHLRADRQRAAECARCLRVLRVNRRTLELYGADDLSQLVDNLHRVFRDDMFEQFV
                     EEMAQLWEGRTRFASQTVNYSLSGTRLDILLNAQVLPGHEDDWSRVLLSIDDVTERVR
                     AERRLAMSELYARGLFEHSPVSLWVEDFRAVKRLLEEVREQGITDFRTFTDVHPEFAE
                     RCMAEIRVIDVNEQTLKMFVAPDRPTLLKSLDQVFRDDMRASFTEQLIDLWQGKLFQQ
                     REVVNYALNGDKLHVLLQFSVLPGHEWDWNLVLVSLTDITARKQAEAYLEFLGKHDVL
                     TKLRNRAYFVDELNRLERKGPYPVTVIVADLDGLKSTNDELGHAAGDALLRRAAEVLA
                     KSVEKPGCAARIGGDEFALLLPGTEERGGEQVMEHIRELIEVNNQFYSGVELSLSLGA
                     ATCRKGERLEAIVNRADQQMYASKKAYYAAGGHDRRHNESASVKKSD"
     gene            complement(3025..>3977)
                     /locus_tag="VFV25_09015"
     CDS             complement(3025..>3977)
                     /locus_tag="VFV25_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393507.1"
                     /translation="AGLNDRWLLGYLDYCCRDDYGAGLAQVSAWAGLHYFASRHGFHA
                     PGEDDADELDAVLTWPEGNAHLAALLGRRNADRAQTASVLTRVTVGRHDVTLDVWRAA
                     AMRSERWIARQVVLSLPLFVAARLIDAPPAALSELAPRLRYAPWLVSNLQLRAPLLER
                     VGAPPAWDNVIQASTTLGYVDAMHQSLRPVPGPTVLTHYWALGGQSPGELQAARHRLL
                     NDPWPIWAEAVIADLSGVHPDLRDKLERIDLMRYGHAMCIPTPGLRGHPALAALGRPQ
                     GRLHFAHADLSAYSVFEEAFTWGDAAGARAAAALRGGRAT"
BASE COUNT          652 a         1356 c         1387 g          582 t
ORIGIN      
        1 ctgacttgca ggccggaacg gccgaggcgg cggtattgca ttgaggggtt ctccgtattc
       61 ggcgatgcgt gaggccagcg ggatcgcggg cgacgctaga ctgcgcagcg tagcctaaag
      121 gtattgaccc atgcagccct ccgtgtacac ggctagacgc ctgcgcgtga tcgaagccct
      181 gcgcgcagcc ggcggcggcg tcgccgtgct gtccaccgcg cccgagcggc agcgcaatgc
      241 cgacaacgat cacccctacc ggcacgacag cagcttctat tacctcaccg gctttgccga
      301 gcccgaggcc tggctggtgc ttaccagcgc cgggcgcagc ctgctgttct gccgcccgcg
      361 cgatgtggag cgcgagatct gggacggcca tcgcctcgga cccgaggccg cgcccgccgc
      421 cctgggtcta gacgaggccc attcgatcgc ggcgctggac gagatgatgc cgcggctgct
      481 cgccgatcag ggggcggtct atttcccctt cagcggctac gaggggctgg gcagccggct
      541 cgccggttgg ctggatcacc tgcgctccca cctcgtgccc gtcggcgcga gtgcgccggt
      601 gcggcaatgc gacctgacgg gcctgatcgg cgagatgcgc ctcatcaagg acgagcacga
      661 gctggcgctg ctgcgccgcg ccggcaccgt ggccgcctcg gcccatgtgc gcgcgatgcg
      721 cgcctgcgcc cacggcctgc gacagccgcc gcccgaaggc ctgcgcgaat accacctcga
      781 ggccgagctg ctctacgagt tccgccgcca cggcacaagc gggccggcct atgccagcat
      841 cgtcgccgcc ggcgccaatg cctgcgtgct gcactacgcg gccggcgacg ccgagctgaa
      901 gagcggcgag ctgtgcctga tcgatgccgg ttgcgaattc ggcagctatg cgagcgacgt
      961 gacccgcagc ttcccgatca gcggccgctt cagcgggccg cagcgtgcgc tctacgagct
     1021 ggtgctggcc gcgcaggagg ccgccatcgc ccacacgaag cctggcgccc gcaagcagga
     1081 tgcgcattgg gccgcggtac gctgcctgag ccagggcctg ctggacctca agctgctgga
     1141 tcgcaagcag catggcggcg tggacgacgt cgtggagaag gccgcctacc gtcgctacta
     1201 catgcacggc actggccatt ggctgggcct ggacgtgcac gatgccggcg aatacctgca
     1261 gctcgacgag gcgcctgtcg agcagcccga cagccgtggc gggcgggtgc tgaagaagcc
     1321 ctcgcggcgg ctgcagccgg gcatggtcgt cacgctggag cccggtctct atgtgcgccc
     1381 ggcagcggac atactgcagc attactggaa tatcggcata cgcatcgagg acgatgccgt
     1441 ggtgacgccc gagggctgcg agttgatcac gcgcggcgcc ccggtgaagc ctgacgagat
     1501 cgaggcgctg atgcgggacg cgacggcatg accaccttgg cttccggcgc ggaccacgag
     1561 ttcgccgaca tgttcgagct cgcgccggta tcgctctggc tcgaggacta caccgagctc
     1621 aagcgcctgt tcgagcgctg gcgcggcgag ggcgtgagcg atctgcgctc gcatctgcgc
     1681 gccgaccggc agcgtgccgc cgaatgcgcg cgctgcctgc gcgtgctgcg cgtgaaccgc
     1741 cgcacgctgg agctctacgg cgccgacgac ctgtcgcagc tggtcgacaa cctgcaccgc
     1801 gtgttccgcg acgacatgtt cgagcagttc gtcgaggaaa tggcccagtt gtgggaaggc
     1861 cgcacgcgct tcgccagcca gacggtcaac tacagcctga gcggcacacg gctcgacatc
     1921 ctgctcaatg cccaggtgtt gcccggccac gaggacgact ggagccgggt gctgctgtcc
     1981 atcgacgacg tgaccgaacg cgtgcgcgcc gagcgccgcc tggcgatgag cgagctctac
     2041 gcgcgcggcc tgttcgagca ttcgccggtg tcgctgtggg tggaggactt ccgcgccgtg
     2101 aagcgcctgc tcgaggaagt gcgtgagcag ggcatcacgg acttccgcac cttcaccgac
     2161 gtgcacccgg agttcgccga gcgctgcatg gccgagatcc gcgtgatcga cgtcaacgag
     2221 cagacgctga agatgtttgt cgcgccggac cggcccacgc tgctgaaatc gctcgaccag
     2281 gtgttccgcg acgacatgcg ggccagcttc accgagcagc tcatcgacct gtggcagggc
     2341 aagctgttcc agcagcgcga ggtggtgaac tacgcgctca acggcgacaa gctgcatgtg
     2401 ctgctgcagt tctcggtgct gccgggccac gagtgggact ggaacctggt gctggtctcg
     2461 ctcaccgaca tcacggcgcg caagcaggcc gaggcctatc tcgagttcct cggcaagcac
     2521 gatgtgctca ccaagctgcg caaccgcgcc tatttcgtcg acgagctcaa ccgcctcgag
     2581 cgcaaggggc cctatcccgt cacggtgatc gtggccgatc tggacgggct caaatcgacc
     2641 aatgacgagc tcggccacgc cgcgggcgac gcactgctgc gccgcgccgc cgaggtgctc
     2701 gccaagtcgg tcgagaagcc gggctgcgcc gcgcgtatcg gcggcgacga gttcgcgctg
     2761 ctgctgccgg gcaccgagga gcgcggcggc gagcaggtga tggagcacat ccgcgagctg
     2821 atcgaggtca acaaccagtt ctatagcggc gtcgaattga gcctgtcgct gggcgcggcc
     2881 acctgccgca agggcgaaag gctggaggcc atagtgaacc gtgccgacca gcagatgtat
     2941 gcctccaaga aggcctacta cgccgcaggc gggcacgatc gccgccacaa cgaatccgcg
     3001 tcggtgaaga agagcgactg accgtcaagt cgccctgccg ccgcgcagcg cggccgcggc
     3061 acgggccccg gccgcatcgc cccaggtgaa ggcctcctcg aagaccgagt aggccgaaag
     3121 gtcggcatgc gcgaaatgca gccggccctg aggccggccc agcgccgcga gcgccggatg
     3181 accgcgcaag ccaggggtcg ggatgcacat cgcatggccg tagcgcatca ggtcgatgcg
     3241 ctccagcttg tcgcgcagat cggggtgcac gccgctcagg tcggcaatca cggcttcggc
     3301 ccagattggc cacgggtcat tgagcaagcg atggcgcgcg gcctgcaact cgcccgggct
     3361 ttgcccgccc agcgcccagt aatgggtcag cacggtgggg ccgggcacgg gtcgcaggct
     3421 ctggtgcatc gcatccacat agccgagcgt ggtgctggcc tggatcacgt tgtcccacgc
     3481 aggcggcgca ccgacgcgct ccagcagcgg cgccctcagc tgcagattgc tgaccaacca
     3541 gggcgcatag cgcaggcgcg gcgcgagttc gctcagggcc gcgggcggtg catcgatcag
     3601 gcgcgcagcg acgaagagcg gcaggctgag cacgacttgg cgcgcgatcc agcgctctga
     3661 gcgcatcgcc gcagcgcgcc acacatcgag cgtcacatcg tggcggccga cagtgacgcg
     3721 ggtcaacacg gacgcggtct gcgcgcggtc cgcgttcctc cggcccagca gcgcggcgag
     3781 atgtgcattg ccttccggcc aggtgagcac ggcatcgagt tcgtcggcat cgtcctcgcc
     3841 cggcgcatga aagccatgcc ggctcgcgaa gtagtgcaag ccggcccagg ccgagacctg
     3901 ggccaggccg gcgccgtagt cgtcgcggca gcagtagtcg agatagccca gcagccagcg
     3961 gtcgttgagg cccgccg
//
LOCUS       DASUCY010000452         3165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_925800, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000452 DASUCY010000000
VERSION     DASUCY010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925800"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="VFV25_09020"
     CDS             complement(<1..419)
                     /locus_tag="VFV25_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEY1393508.1"
                     /translation="MRFSTKAIHAGQPADPSTGAIMTPVYLSSTFVQEAPGRHKGYDY
                     ARTANPTRSALEANLAALEEGAWGLAFASGLAAESTLLQTLSSGDHVVCGQDLYGGTY
                     RLMTRVFERFGIRTSFVDATSLDAIQAALRPQTRLVWL"
     gene            complement(448..1458)
                     /locus_tag="VFV25_09025"
     CDS             complement(448..1458)
                     /locus_tag="VFV25_09025"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEY1393509.1"
                     /translation="MKYESILDAIGHTPLVRLNQLSRHLPCAIYAKLEFLSPGGSVKD
                     RIGMYMIQQAERRGEIGPGATIVECTSGNTGMGLALFAAGRGYKTVFTIADKQSKEKI
                     DMLRAMGAHVLVCPTDVPPDDPRGYIQVAQRLAKDIPGAFLCNQYDNPDNTLAHYAST
                     GPEIWDDTGGMVTHFVCGMGTCGTISGAGRYLKEQNAMVRVIGVDPQGSIFHELFHRG
                     VTVQPHVYRLEGIGEDFIPKALDWHAIDDVIQVSDQDSFLMARRLARAEGIFAGGSSG
                     AAVLAALQVAEKLGPNDLVVVLLPDGGRQYLGKLYDDDWMRDHGYLNAPPGAAPPAAG
                     QP"
     gene            complement(1876..>3165)
                     /locus_tag="VFV25_09030"
     CDS             complement(1876..>3165)
                     /locus_tag="VFV25_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY1393510.1"
                     /translation="VSHFKEAEGWVPAAPADALERGPWWSLFGDPVLDQLASRVEVSN
                     QNVAAAVAAYAQARALVREQRASLFPSVTLDGGASRSRSGGASTGGRIGNNYQLSIGG
                     SWEPDVWGRLGHAVDSATAGAQASAADLASARLSAQGELAANYLSLRQTDAQKALLES
                     TIAGYQRTLEITQNRYAAGIAAKTDVLQAQTQLANAQADEAGLVRQRAQLEHAIAVLT
                     GEAPGNFSLAPAAWNPVVPEIPVGLPSTLLQRRPDIAAAERRVAAANEQIGIAKSAYY
                     PSLALSASYGFGASRVADLFSASSSVWSIGLSAVQVLFNAGATGARVEGSEAAHAQAV
                     ARYRQTVLAAFQSVEDQLAATRVLLTQQDLRRQASLAADQVEQQVLNRYRSGQVSYTE
                     VITAQATALSARRALVQAMADRQTTAVALIQSLGGGW"
BASE COUNT          440 a         1033 c         1152 g          540 t
ORIGIN      
        1 agccagacca gccgggtttg cgggcgcagc gcggcctgta tcgcgtcgag cgaagtcgcg
       61 tcgacgaagc tggtccggat gccgaagcgc tcgaacacgc gcgtcatcag ccggtaggtg
      121 ccgccataca ggtcctggcc gcagaccaca tggtcgccgc tgctgagggt ttgcagcagg
      181 gtggattccg ccgccaggcc gctggcgaag gccaggcccc aggccccttc ctccagtgcc
      241 gccaggttcg cctcgagcgc gctgcgcgtg gggttggcgg tgcgcgcgta gtcgtaaccc
      301 ttgtggcggc cgggcgcttc ctgcacgaag gtcgagctca ggtataccgg cgtcatgatc
      361 gcgccggtgc tcgggtccgc cggctggccg gcgtgaatgg ctttggtgga aaagcgcatg
      421 gcgtgcctcg ctggggttga aaaaagtcta gggctggccc gcggccggtg gtgctgcgcc
      481 gggcggggcg ttcaggtagc catggtcccg catccagtcg tcgtcgtaga gcttgcccag
      541 gtattggcgg ccgccgtcgg gcagcagcac caccaccagg tcgttcgggc ccagcttttc
      601 agccacctgc agcgcggcca gtaccgcggc gcccgaggag ccgccggcga agatgccctc
      661 ggcgcgggcg agccggcgcg ccatcaggaa gctgtcctgg tcgctgacct ggatcacgtc
      721 gtcgatggcg tgccagtcca gtgccttggg gatgaagtct tcgcctatgc cttcgagccg
      781 gtagacgtgg ggctgcaccg tcaccccgcg gtggaacagc tcatggaaga tcgagccctg
      841 cggatcgacg ccgatcaccc ggaccatggc gttttgctcc ttgaggtagc gaccggcgcc
      901 gctgatggtg ccgcaagtgc ccatgccgca gacgaaatgg gtgaccatgc ccccggtgtc
      961 gtcccagatt tcggggccgg tgctcgcgta gtgcgccagc gtgttgtccg ggttgtcgta
     1021 ctggttgcag aggaaggcgc cggggatgtc cttcgccagc cgctgcgcca cctggatgta
     1081 tccccgtgga tcgtcgggcg gcacgtcggt ggggcagacg aggacgtgcg cccccatggc
     1141 ccgcagcatg tcgatttttt ccttggactg cttgtcggcg atggtgaaca ccgtcttgta
     1201 gccgcgtccg gcggcgaaca gcgccagacc catgccggtg ttgcccgagg tgcattccac
     1261 gatggtggcg ccggggccga tctcgccgcg ccgctcggcc tgctggatca tgtacatgcc
     1321 gatgcggtct ttcaccgagc cgcccggact caggaactcc agcttggcgt agatggcgca
     1381 gggcaggtgc cggctgagct ggttcaggcg caccagcggc gtgtggccga tcgcgtccag
     1441 gatgctttca tatttcatgg cagctccatt tgcgtgttgc gacatgcgag cccggccgcg
     1501 agagcggcat gggctggtca tccactgtag tcgcttcgcg ccggttcgtg aagccgctcg
     1561 gccatccgtt ctacctcctg cccggaaccg ggccgccggg tcaacgggag ggcgttcagg
     1621 ctggggcgtc ggacgccgga cggatccggg cgcagctcag gcaggctcgc cgtcaagacc
     1681 catgcgcctg catctgtcgc tgcgtcgatg gcatggcccg tcctgacgcc gcggtcgatg
     1741 cgctttctgt cggtgccgtg tggcgcggtt ttgccgggtt cttatgggaa aacagtgcac
     1801 agcccaatgg gtgtgggcgt aatgcgctat caaaaccata gtgactgccg gcgcgtggcc
     1861 gctttaccgt ccattctacc aaccgccgcc cagcgactgg atcaaggcca cggcggtggt
     1921 ttggcggtcg gccatggcct gcaccagcgc gcggcgtgcg ctcaacgcgg tggcttgggc
     1981 ggtgatgacc tcggtgtagc tgacctggcc gctgcggtag cggttgagca cctgctgctc
     2041 cacctggtcg gcggccagag aggcctggcg gcgcaagtcc tgctgggtca gcagcacgcg
     2101 ggtggcggcc agctggtctt cgaccgactg aaaggcggcg agcacggttt gccggtagcg
     2161 cgccactgcc tgcgcgtgcg cggcctcgct gccttccacg cgagcgccgg tggcgccggc
     2221 gttgaaaagc acttgcacgg ccgacaggcc gatcgaccag acgctgctgg aggcactgaa
     2281 caggtcggcc acacggctgg cgccgaaacc ataggacgcg ctcaaggcga ggctggggta
     2341 gtaggcgctt ttggcgatgc cgatctgctc gttggccgcg gcgacgcggc gctcggcggc
     2401 ggcaatgtcc gggcggcgct gcagcagcgt ggagggcagg ccgacgggga tctcgggcac
     2461 cacggggttc caggccgcgg gcgccagcga gaaattaccg ggcgcctcgc ccgtcagcac
     2521 ggcgatggcg tgttcgagct gggcgcgctg gcgcaccaac ccggcctcgt cggcctgcgc
     2581 attggccagc tgcgtctggg cctgcagcac atcggttttg gcggcgatgc cggcggcata
     2641 gcggttttgc gtgatctcca gggtgcgctg gtagccggca atggtgcttt cgagcagcgc
     2701 cttttgtgca tcggtctggc gcagcgacag gtagttggcc gccaactcgc cctgggccga
     2761 cagccgggcc gaggccaggt cggccgcgct ggcctgggcg ccggccgtgg cgctgtcgac
     2821 tgcgtggccc agccggcccc agacatcggg ctcccagctg ccgcctatgc tgagctggta
     2881 gttgttgccg atgcgcccgc ccgtgctggc gccgccgctg cgcgaacggc tggcgccgcc
     2941 gtcaagcgtg accgaaggaa acagcgaggc ccgctgttcg cgcaccagcg cgcgcgcctg
     3001 ggcataggcg gccacggcgg cggccacgtt ctggttggag acctccacgc gagacgcgag
     3061 ctggtcaagc accggatcgc cgaacagcga ccaccacggg ccgcgctcca gcgcgtcggc
     3121 gggcgcggcg ggcacccagc cctcggcctc cttgaagtgg ctgac
//
LOCUS       DASUCY010000453         2745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_331457, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000453 DASUCY010000000
VERSION     DASUCY010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331457"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VFV25_09035"
     CDS             <1..321
                     /locus_tag="VFV25_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY1393511.1"
                     /translation="LSAAQGQSISTIEALANAAPLGPALQQAWVEHQVPQCGYCQSGM
                     LMAAAALLQHNLRPSDADSDAAMTNLCRCGSYPRVRAAIHAAAASLAGKPQDGDVSHS
                     RVPA"
     gene            431..2743
                     /locus_tag="VFV25_09040"
     CDS             431..2743
                     /locus_tag="VFV25_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY1393512.1"
                     /translation="MKRRHLLLGAAAAGGALLVGWAVLPPRSRLGSQDLLSAAPGEIS
                     LNGWIKIAADGTVVLAMHRSEMGQGVHTALAQLVAEELDIPLARIRLIQAGADAIYGN
                     VAMFIGSLPFHPSDSEPGRETGAVKAGRWFVGKLARELGVNATGGSSTIADAWEPLRW
                     AAATARAQLVNAAALLWKLPSNELEVKEGVVSHGSGPHAHFGELAKTAAAAPIGEVRL
                     KERERWTVIGRAAPRIDTLAKSRGEARFGLDVRLPGMVYAAVRMAPMLGGAPGRIVNE
                     DEVLRRPGVERLVRLPPAAGSTAGVAIVARSTWHAREALRALEIEWQARPAGPLDSAA
                     IMARLEAKAREAAASEGGFAFYKRGDVAAAERSSNSGARTVEAVYRAPYLAHAAMEPI
                     NCTAQLKDGRLTVWAPTQVPSFARSVAARTASLAEDKVDVQVTYLGGGFGRRLDVDFV
                     AQAMVVAQETGGRPVQLVWSREEDITHDFYRPAAVAVLKATLAADGKPQSLRCTSAGD
                     AITPRWLERGLPALTGPVDLPDKTTMEGLYDLPYAVPNQRMAHAATTSGVPIGFWRSV
                     GHSHNAFFSESFIDELAHAAQQDPVQYRLALLAEMPRHAAVLKLAAEKAGWGQPLPAG
                     RARGVALHESFGSIVAEVLEVSLQADGKPRVHRVVCAIDCGTAVNPGIIAQQMESGIV
                     FALGAALYSRIDIVNGAVQQNNFPNYPLLSLRETPVIETHILPSTLPPTGVGEPGTPP
                     VAPALANAIFVLTGQRLRELPLKLSWARSQ"
BASE COUNT          408 a          976 c          980 g          381 t
ORIGIN      
        1 ttgtcggcgg cacaggggca gagcatctcc accatcgagg ccctggcgaa cgccgcgccg
       61 ctaggcccgg cgctgcagca ggcctgggtc gagcatcagg tgccgcaatg cggctattgc
      121 cagagcggca tgctgatggc cgccgcggcg ctgctgcagc acaacttgag gcccagcgat
      181 gccgacagcg acgcggcgat gaccaacctc tgccgctgcg gcagctatcc gcgcgtgcgc
      241 gccgccatcc acgcggcggc ggcctcgctg gccggcaagc cgcaagacgg cgacgtcagc
      301 cattcgcggg tgccggcatg agacatccag tgtcaacctg tcttccgcgg gccgcgcgtc
      361 gggccgttcc caaaccggcc cgcatcccct cgggggatcg tcgaacgtac tcgctcgacg
      421 aggggtcgtc atgaagcgtc gccatctgct gttgggcgcg gcggccgctg gcggtgcgct
      481 gctggtgggc tgggctgttc tgccgccgcg ctcgcgcctg ggcagccagg acttgctgag
      541 cgccgcgccg ggcgagatca gcctcaacgg ctggatcaag atcgccgccg atggcaccgt
      601 ggtgctggcg atgcatcgca gcgagatggg ccagggcgtg cacaccgcgc tggcccagct
      661 ggtggccgag gagctggaca ttccgctggc gcgcatccgc ctgatccagg ccggcgccga
      721 cgccatctac ggcaatgtcg cgatgttcat cggcagcctg cccttccacc ccagcgacag
      781 cgagccaggg cgcgagaccg gcgccgtcaa ggccggccgc tggttcgtcg gcaagctggc
      841 acgcgagctg ggcgtcaacg ccaccggcgg ttcgtccacc attgccgatg cctgggagcc
      901 gctgcgctgg gccgccgcca cggcccgggc tcagctcgtc aacgcggccg ccttgctgtg
      961 gaagctgcca tccaacgagc tggaagtgaa ggaaggcgtg gtcagccatg gctcggggcc
     1021 gcacgcgcat ttcggcgagc tcgccaagac ggccgccgcc gccccgatcg gcgaggtgcg
     1081 cctgaaggag cgcgagcgct ggaccgtgat cggccgcgca gcgccgcgca tcgatacgct
     1141 ggccaagagc cgcggcgaag cccgcttcgg cctggacgtg cgtctgcccg gcatggtcta
     1201 tgccgcggtg cgcatggcgc cgatgctggg cggcgcaccc gggcgcatcg tcaacgaaga
     1261 cgaggtgctg cgtcgccccg gcgtggagcg tctggtgcgc ctgccgccgg ccgcggggtc
     1321 cacggccggc gtcgccatcg tcgcgcgcag cacctggcac gctcgcgagg cgctgcgagc
     1381 gctggagatc gaatggcagg cgcgcccggc cgggccgctc gattcggcgg ccatcatggc
     1441 ccggctggag gccaaggccc gcgaggccgc ggcgagcgaa ggcggcttcg ccttctacaa
     1501 gcgtggcgac gtggccgcgg ccgaacggag cagcaacagc ggcgcacgca cggtggaagc
     1561 cgtctaccgc gccccttacc tggcccatgc cgcgatggag ccgatcaact gcacggcgca
     1621 gcttaaggac ggccgcctca cggtctgggc gccgacccag gtgccgagct tcgcgaggtc
     1681 cgtggcggcg cgcaccgcca gcctcgccga ggacaaggtc gatgtgcagg tgacctatct
     1741 cggcggcggc ttcggccgcc ggctcgacgt ggacttcgtc gcccaggcga tggtggtggc
     1801 gcaagagacg ggcggccgcc cggtgcagct cgtgtggtcg cgcgaggagg acatcaccca
     1861 cgacttctat cgcccagccg cggtggccgt gctgaaggcc accctcgcgg ccgacggtaa
     1921 gccgcagagc ctgcgctgca ccagtgccgg cgatgcgatc acgccgcgct ggctggaacg
     1981 cggcctgccg gcactgaccg gcccggtgga cttgcccgac aagactacga tggagggcct
     2041 gtacgacctg ccctatgccg tccccaatca gcgcatggct cacgccgcga cgacgagcgg
     2101 cgtgccgatc ggcttctggc gctcggtcgg gcactcgcac aacgccttct tcagcgaaag
     2161 cttcatcgac gaactggctc atgcggcaca gcaagacccg gtgcaatacc ggcttgcgct
     2221 gctggccgag atgccgcgcc acgcggcggt gctgaagctc gccgccgaga aggcaggctg
     2281 gggccaaccg ctgccggccg ggcgtgcgcg cggcgtcgcg ctgcacgaga gcttcggctc
     2341 catcgtggcc gaggtcctgg aggtgtcgct gcaggccgac ggcaagccgc gcgtgcatcg
     2401 cgtggtctgc gcgatcgact gcggcaccgc ggtcaacccg ggcatcatcg cccagcagat
     2461 ggaaagcggc atcgtcttcg ccctcggcgc cgcgctctac agccgcatcg acatcgtgaa
     2521 cggcgccgtg cagcagaaca acttcccgaa ttacccgctg ctgagcctgc gcgaaacacc
     2581 cgtcatcgaa acccacatcc tgcccagcac cctgccgccg accggcgtcg gcgagcccgg
     2641 cacaccgcca gtggcgccgg cgctggcgaa tgcgattttt gtgctgacgg ggcagcgctt
     2701 gcgcgagctg ccgttgaagc tcagctgggc gcggtctcag tgaag
//
LOCUS       DASUCY010000454         2426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1423968, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000454 DASUCY010000000
VERSION     DASUCY010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1423968"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VFV25_09045"
     CDS             complement(<1..441)
                     /locus_tag="VFV25_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393513.1"
                     /translation="MDMSDLGQSKRGGGIDAVALPADDLDNERWRALITRIGCEIAEP
                     LSAALERIIGLATSGSIDTYNLRALRREVEHARKAGIASQQLARFASGRLKQSHERLH
                     LTQTLQSVLGLRTAEVQARGIQVKQVLRPVEVIVDPSLLFSLLNT"
     gene            complement(655..1899)
                     /locus_tag="VFV25_09050"
     CDS             complement(655..1899)
                     /locus_tag="VFV25_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY1393514.1"
                     /translation="MTDTLPTSPAALEGLRVLDLSRVLAGPWCTQILGDLGADVVKIE
                     RPGSGDDTRGWGPPFLPDRDGHASAESAYYLGANRNKRSLTVDISRAEGQALIRELAA
                     KADVFVENFKVGDMARYGLDYASLAALNPRLVYCSITGYGQTGPYRDRAGYDYAIQGI
                     GGLMGVTGERDDLPGGGPQKVGVAVADLFTGLYATVAVLAALRHRDATGQGQYIDMAL
                     LDTQVAMLANLGANYLVSGKVPGRLGNAHQNIVPYQVFEVADGHLILAVGNDGQFAKF
                     CTVAGCPELAEDPRYAKNADRVRNRALLVPLLAQVLKTRTRADWLQALEAAKVPCGPI
                     NGLDEVFADPQVVARGMTEAVPHPLNDALRLVASPLKLAATPARTRHAPPLLGQHSEE
                     VLADWLACDAARMQALRDAGVI"
     gene            1962..>2426
                     /locus_tag="VFV25_09055"
     CDS             1962..>2426
                     /locus_tag="VFV25_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393515.1"
                     /translation="MFPLPKTRPALLVAALATAVAACSPALNWREVRPESTALLAMFP
                     CKPGQQERSLPLAGAMVQMRLYACEAEGASWALSHADVGDPARVGPALQELRAALLQH
                     LGRRGASAPPSPFSVPGMTPQAEAQRFTLRGERGGDGKAMELQVGLFSQGSVV"
BASE COUNT          322 a          832 c          895 g          377 t
ORIGIN      
        1 cgtgttgagc aggctgaaca gcagcgaggg atcgacgatc acctcgaccg ggcgcagcac
       61 ctgcttgacc tggatgccgc gcgcctgcac ttccgccgtg cgcagcccca gcacgctctg
      121 cagcgtctgg gtcaggtgaa ggcgttcgtg cgattgcttg agccgcccgg aggcgaagcg
      181 cgccagctgc tggctcgcga tgccggcctt gcgcgcgtgc tcgacttcgc ggcgcagtgc
      241 gcgcaggttg taggtgtcga tgctgccgct ggtggcgagg ccgatgatgc gttccagcgc
      301 ggccgacagc ggctcggcga tctcgcagcc gatgcgcgtg atcagcgcgc gccagcgctc
      361 gttgtccaga tcgtcggcag gcagtgccac ggcgtcgatg ccgccgccgc gcttgctctg
      421 gcccaggtcc gacatatcca tggacatcct cgttgcaccc cgttggacgc ggcgccggcc
      481 gcgggagccg ccccgcaacc tgttgttctg tgtgacagca gtgtttcagc tgcgccggga
      541 ggcgtccatc cctcgactaa gggccggggg cgacctggcc cggtccgctg cttgctggca
      601 tggatgacag cacccggtcg cggcttaccg cgaccaccct ccgcggggca tcggctaaat
      661 cacgccggca tcgcgcaggg cttgcatccg cgccgcatcg caagccagcc aatcggccag
      721 cacttcctcg ctgtgctggc cgagcagcgg cggggcgtgc cgggtgcgcg caggcgtggc
      781 ggcgagcttg agcgggctgg cgaccaggcg cagcgcgtca ttgagcggat gcggcacggc
      841 ctcggtcatg ccgcgcgcga cgacctgcgg atcggcgaag acctcgtcca gcccattgat
      901 cggcccgcac ggcactttgg cggcttcgag cgcttgcagc cagtcggccc gcgtgcgcgt
      961 cttgaggact tgcgccagga gcggcaccag gagcgcgcgg ttgcgcacgc ggtcggcgtt
     1021 cttggcatag cgcgggtcct cggccagctc ggggcagccg gcgacggtgc agaacttggc
     1081 gaactggccg tcgttgccca cggccaggat caggtgcccg tcggccacct cgaagacctg
     1141 gtagggcacg atgttctggt gcgcattgcc cagccgcccc ggcaccttgc cgctgaccag
     1201 gtagttggcg cccaggttgg cgagcatcgc gacctgggtg tcaagcagcg ccatgtcgat
     1261 gtactggccc tggccggtgg cgtcgcgatg gcgcagcgcg gccagcacgg ccaccgtggc
     1321 atacagcccg gtgaagaggt cggcgaccgc gacgcccacc ttctgcgggc cgccgccggg
     1381 caggtcgtcg cgctcgccgg tgacgcccat cagcccgcct atgccttgga tcgcatagtc
     1441 gtagccggcg cggtcgcggt aggggccggt ctggccgtag ccggtgatgg agcaatagac
     1501 gaggcgcggg ttcagcgcgg ccaggcttgc gtagtccagg ccatagcgcg ccatgtcgcc
     1561 gaccttgaag ttctcgacga agacatcggc cttcgccgcc agctcgcgga tcagcgcctg
     1621 gccctccgcg cggctgatat ccaccgtgag cgagcgcttg ttgcggttgg cgcccaggta
     1681 ataggccgat tcggcgctgg catggccatc gcggtccggc aggaagggcg ggccccagcc
     1741 gcgcgtgtcg tcgccgctgc cgggacgctc gatctttacg acatcggcgc ccaggtcgcc
     1801 gaggatctgg gtgcaccagg gaccggccag cacgcgcgag aggtcgagca cgcgcaggcc
     1861 ctcgagggcg gccggcgagg taggcagggt gtcggtcatc gctgcatttt gctgtagcgc
     1921 tgcgccgggc cgctgccgcg gccggcccgg ataatccgcc catgttcccg cttcccaaga
     1981 cacggccggc gctgctggtg gctgcgctgg cgacggccgt ggcagcctgc tcgccggctc
     2041 tcaattggcg cgaggtgcgc cccgaatcaa cggcgctgct ggcgatgttc ccctgcaagc
     2101 ccggccagca ggagcgcagc ctgccgctgg ccggggccat ggtgcagatg cggctgtacg
     2161 cctgcgaggc ggagggcgcc agctgggccc tgagccatgc cgacgtgggc gacccggcgc
     2221 gcgtcgggcc ggcgctgcag gaactgcgcg cggcgttgct gcaacacctg ggcaggcgcg
     2281 gagcgagtgc gccgccctcg cccttttcgg tgcccggcat gacgccgcag gccgaggccc
     2341 agcgcttcac gctgcgcggc gagcgtggcg gcgacggcaa ggcgatggag ttgcaggtcg
     2401 ggctgttcag ccagggctcg gtcgtc
//
LOCUS       DASUCY010000455         2198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1520175, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000455 DASUCY010000000
VERSION     DASUCY010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1520175"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="VFV25_09060"
     CDS             <1..511
                     /locus_tag="VFV25_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY1393516.1"
                     /translation="TALIYARRNEAFRERFDAMWLTLPLIGRLARGYNAARFGGTLAM
                     LAGAGVPILKALQAAAETLGNRAMRADAMEALVQVREGAPLASALAAKKRFPGLLPMF
                     ARLGEQTGELPLMLQRAAKQLSAEVQRRAMALATILEPLLIVGMGVIVMLIVLAVLLP
                     IIQLNDWVK"
     gene            699..1601
                     /locus_tag="VFV25_09065"
     CDS             699..1601
                     /locus_tag="VFV25_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HEY1393517.1"
                     /translation="MPATLDGQLVVAISSRALFDFEEENQVFEGSDDRAYMQLQLQRL
                     EKPARPGVAFSLVHKLLAFNTEERQRVEVVILSRNDPVSGMRVFRSAQHFGLPIQRGV
                     FTRGSSPYRYLTPLHANLFLSANEADVRSALDAGVPAARVAPHSARASDAHPHEVRIA
                     FDGDAVLFSDEAERVFQSGGLDAFQRSEADLAAKPLAAGPFKPLLEALQGLQQAGTQR
                     MRIRTALVTARSAPAHERAIRTLMQWNIEVDEAMFLGGLNKGEFLREFEPDFFFDDQT
                     RHVEHAANHVPAGHVAAGVANAKT"
     gene            complement(1676..>2198)
                     /locus_tag="VFV25_09070"
     CDS             complement(1676..>2198)
                     /locus_tag="VFV25_09070"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HEY1393518.1"
                     /translation="YGSRPTDPTGLVAGPSFTGGDLYSYLNLITSTPASMNFVNMSGG
                     ESLAGLTTPTSFSIYEFIVTVPTAAVGATLNLPAQFNIPLLLQLGDYVAGWDLASNNH
                     FYATPFTTAGLVTTTSSTSSSGISSTGSTSSTSSSSGISSTGVPEPNSSSLALLGLGL
                     LAASFWVRRRTQL"
BASE COUNT          347 a          743 c          744 g          364 t
ORIGIN      
        1 cacggcgctg atctatgcgc ggcgcaacga ggccttccgc gagcgctttg acgcgatgtg
       61 gctcacgctg ccgctgatcg ggcggctcgc gcgcggctac aacgcggcgc gcttcggcgg
      121 cactctggcc atgctggccg gcgccggcgt gcccatcctg aaggccctgc aggccgcggc
      181 cgagacgctg ggcaaccgcg cgatgcgcgc cgacgcgatg gaggcgctgg tgcaggtgcg
      241 cgaaggtgcg ccgctggctt cggcgctggc cgccaagaag cgcttccccg gcttgctgcc
      301 catgttcgcc cgcctgggcg agcagaccgg cgagctgccg ctaatgctgc agcgcgccgc
      361 caagcaattg agcgccgaag tgcagcgccg cgccatggcg ctggccacca ttctcgagcc
      421 gctgctgatc gtcggcatgg gcgtgatcgt gatgctcatc gtgctggccg tgctgctgcc
      481 gatcattcag ctgaacgact gggtcaaata agcccacccc cgttgcgctt cgcgctcccc
      541 ctcaaggggg ccacgccgac ggcccggtaa agccggatcc gtggcgcgtg cttggatgtg
      601 cggtgttgct cccatagtcc gttccagtcg tcgtgctgcg aacgaggtga gctgctggct
      661 ccatgagccg tcgttcattg catggctatc atccgcctat gcccgcaaca ctggacggcc
      721 agttggtcgt tgccatctcc tcgcgcgcgc tgttcgactt cgaagaagaa aaccaggtct
      781 tcgaaggcag cgatgaccgc gcctacatgc agctgcagtt gcagcgcctc gagaagccgg
      841 cgcggcccgg cgttgccttt tcgctggtgc acaagctgct cgccttcaac accgaggaac
      901 ggcagcgcgt cgaggtcgtg atcctgtcgc gcaacgaccc ggtctcgggc atgcgcgtgt
      961 tccgctcggc ccagcatttt ggcctgccga tccagcgcgg cgtgttcacg cgcggcagct
     1021 cgccctatcg ctacctgacg ccgctgcacg ccaacctctt cctctccgcc aacgaagccg
     1081 acgtgcgctc ggcgctggac gccggcgtac cggccgcgcg cgtcgcgccg cattcggcgc
     1141 gcgcctcgga tgcccaccct cacgaagtgc gcatcgcctt cgacggcgac gccgtgcttt
     1201 tctccgacga ggccgagcgc gtgttccaga gcggcgggct cgacgccttc cagcgcagcg
     1261 aggccgacct agccgccaag ccgctcgccg ccggcccctt caagccgctg ctcgaggcgc
     1321 tgcagggcct gcagcaggcc ggcacgcagc gcatgcgcat acgcacggcg ctggtcaccg
     1381 cgcgcagcgc gccggcccac gagcgcgcga tccgcacgct gatgcaatgg aacatcgagg
     1441 tcgacgaggc gatgttcctc ggcggcctga acaagggcga attcctgcgc gagttcgagc
     1501 ccgacttctt cttcgacgac cagacgcggc atgtcgagca cgcggccaat catgtgccgg
     1561 cggggcatgt ggcggcgggg gtcgcgaatg cgaagacctg aagccgcatg cggtgtagcc
     1621 ccaaaatgac cgaccccgcg gttgcggggt cgggtgactt catgcgggac agggtttaca
     1681 gctgggtgcg gcggcggacc cagaagctcg ctgcaaggag cccgaggccg agcagcgcca
     1741 agctgctgga attcggctcc ggaacgcccg tgctcgaaat gccactggag ctgctagtgc
     1801 tgctggtgct gccggtactc gaaatgccgc tggagctcgt gctgctggtc gtcgtcacca
     1861 gcccggcggt ggtgaacggc gtggcataga aatgattgtt cgaggccagg tcccagccag
     1921 cgacgtagtc gcccaactgc aggagcagcg gaatattgaa ttgcgctggc aaattgagag
     1981 tcgcgccaac ggccgccgtc ggcacggtga cgatgaactc gtagatcgag aaggatgtcg
     2041 gcgtggtcag ccccgccagt gactccccgc cgctcatgtt gacgaagttc atcgaggccg
     2101 gcgtgctcgt gatcaggttc aagtacgagt acagatcgcc cccggtgaaa gagggccccg
     2161 cgaccagccc ggtcgggtcg gtgggcctgg agccgtag
//
LOCUS       DASUCY010000456         8825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_877648, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000456 DASUCY010000000
VERSION     DASUCY010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 8825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8825
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877648"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VFV25_09075"
     CDS             complement(<1..418)
                     /locus_tag="VFV25_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HEY1393519.1"
                     /translation="MMTLAQALALLPGATLVGGATQAGALQIKRVHSDTRSLQPGDLF
                     VAIVGERFDAHAFLLEAKAAGAIAALAQRGLAEAGLPGLQVTDSKQALGELAAAWRAQ
                     FSLPLIAVTGSNGKTTVTQMIASILRAWLGPAAFWTE"
     gene            complement(418..1902)
                     /locus_tag="VFV25_09080"
     CDS             complement(418..1902)
                     /locus_tag="VFV25_09080"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEY1393520.1"
                     /translation="MLTRLKSPQAAARWLREWASKGTLRTDSRQVQPGDAFIAWPGYA
                     TDGRKYVPAALAAGAATCVVEAEGVEAFGFDDARIAALPGLKHATGPLAAHYFGDPTQ
                     ELDVVAVTGTNGKTSSSWWVAQALNALGRGAGVVGTLGVGVPPQVRSTGLTTPDPVTL
                     QAAFRDFVDRGYEAASIEASSIGIVEGRLTGTRIAAALFTNFTRDHLDYHGTMEAYWA
                     AKAELFAWPGLKAAVVNVDDEQGAKLAVDLAAESKLDVWTYSLSRPARLQASRLGYRD
                     GGLSFVLREGEQEVQVRTTLIGDYNASNLLAVLGGLRALDIPLADAAGICPLLTPVPG
                     RMQRVGGTAGLPVAVVDYAHTPDALDKALQAVRSLARQGGGQLWCVFGCGGNRDATKR
                     PLMGGIAARAADRVVITSDNPRDEEPAQILAQIQSGVARGDGVEVIEDRRVAIIRTLQ
                     QAAPGDVVLIAGKGHEDYQEVRGVKLPFSDVQVAEEVLSARRPA"
     gene            complement(1902..3683)
                     /locus_tag="VFV25_09085"
     CDS             complement(1902..3683)
                     /locus_tag="VFV25_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEY1393521.1"
                     /translation="MSWFKREPKRQAGATSRSVRYATSPLLAAKTPPWRSKLLVVGIG
                     LGFCLLVGRALYIQVLGTDFYLKQGESRYARTLELPANRGRIIDRTGLLLASSVPAPS
                     IWALPKDLDANKTQLRALAKLLAMTPAELDKRLGENQNFVWLRRQVDESVAAQVKQLG
                     IKGVYQLQEYKRKYPEGEAAAHVVGFTNVEDKGQEGVELAFQKELAGRDGTRRVIKDR
                     LGRVLEDIGDSTPPMDGQDIDLSIDSKVQFFAYQRIRDAVTENQAKAGSVVVLDAQTG
                     EVLALANYPSYSPSNRQNLAGGQLRNRALTDTFEPGSTMKPFTIALALETGRVTPQTV
                     IDTSPGRMTITGSTISDAHPHGALTVEQVIQKSSNVGTAKIAMRMQPREMWEMFASAG
                     IGQRPQLPFPGAVTGRLRPYKSWRPIEQATMSYGYGLSVSLFQIAHAYTAFARDGELI
                     PVTLTKRDQPVGGTRIMSAQTASAIRHMLEMSTDNGGTSPQARTIGYSVGGKSGTAYK
                     QEGKTYNTKKYRSWFVGMAPINAPRIIVAVMVDEPSAGKHFGGEVAGPVFSEVVQQTL
                     RMMGVQPDLEIKPQIVAQKMQAEEESF"
     gene            complement(3680..3973)
                     /gene="ftsL"
                     /locus_tag="VFV25_09090"
     CDS             complement(3680..3973)
                     /gene="ftsL"
                     /locus_tag="VFV25_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEY1393522.1"
                     /translation="MSRINLLLLLALIVSSLYLVKVSYEGRRLYAELDRAKVEEHRLD
                     TDYERLQVEQRAQATPLRVEKVAREKLGMRTANPAVTEYVVPQNSAASARAAQ"
     gene            complement(3970..4935)
                     /gene="rsmH"
                     /locus_tag="VFV25_09095"
     CDS             complement(3970..4935)
                     /gene="rsmH"
                     /locus_tag="VFV25_09095"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEY1393523.1"
                     /translation="MEAFDAGPWQHRTVLLHEAVDALVSSPDGVYVDGTYGRGGHSRL
                     VLSRLSKEGRLIAFDKDPDAIAHASQGKTRVSDPRFAIEHASFAELGRALAERGIAAV
                     QGVLLDLGVSSPQIDNPARGFSFRFDGPLDMRMDPTRGQSAADFLASAPERRIAEVIR
                     DYGEERFAVSIAKALVARRESGRAVQSTAELSEVVARAVKTREPGQDPSTRTFQALRI
                     LVNAELEELEQGLNAALAALAPKGRLVVISFHSLEDRIVKTFIARHSKHEVDRRAPFA
                     EPKPSLLKALARIKPSAAEISENPRARSAILRVAERTDAPLGAAA"
     gene            complement(4942..5370)
                     /gene="mraZ"
                     /locus_tag="VFV25_09100"
     CDS             complement(4942..5370)
                     /gene="mraZ"
                     /locus_tag="VFV25_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019255554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEY1393524.1"
                     /translation="MFQGASALALDAKGRLSVPARHRDMLQAIAEGQLTVTKHPDGCL
                     MIFPRPAWESFRERIAALPMSASGWKRIFLGSAMDVEIDASSRVLISPELRVAAGLSK
                     DVMLLGMGSHFELWDAQRHAEHEAEVMQQGMPEVLKDFSF"
     gene            5837..8374
                     /locus_tag="VFV25_09105"
     CDS             5837..8374
                     /locus_tag="VFV25_09105"
                     /inference="COORDINATES: protein motif:HMM:NF028467.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY1393525.1"
                     /translation="MGQDDEAAAALGERDAAQMDDDRPAYLLSQVAGRAQQLRAAVVL
                     LVCAAVFAALAPFAQLKLAPVPAFIPMYEAALVVTDVITAVLLFGQYRVLRSPAFALL
                     ASGYLFTALMAMSHALTFPGLFAPEGLLGAGQQSTAWIYMFWHAGFPLFVLGYVWLRR
                     NESAIGAARVRLIAGTSPLLSVLVTGGLVALATAGETLLPAIMVGNHYSPSMLGTVAS
                     VWLFSLAALAALWALRSRTVLDTWLIVVIVVWLFDIALSAMLNAGRFDLGFYAGRSFG
                     LLACSVVLLELLLENGMLHARLAQAHSAERLRNRELAIARDAAQAANSAKSLFLASMS
                     HEIRTPMNAIIGLTQLLAETKLDDRQHDFLGKVQTSSRALLSLLNDILDYSKVEAGKI
                     ALEVEEFSPEETIENVGNLFGAKIEEAGLELLFKIDEAIPQRLLGDSLRLAQVLNNLV
                     GNAIKFTPHGEIVIGAEVMARDSSRVELRFSVRDTGIGLTPDQIQRLFEVFAQADRSI
                     SRKYGGTGLGLAICRKLVELMGGRIWAQSTPGQGSSFFFTACFELPSQRAERIDLHRI
                     RGMRTLVLDGQPTARLILQQTLQNWRFQVGTASFADDALHKLRRAEPQAAYELLLLDW
                     KDAGLELVHEARRITAVRTPTPLAVVAMATLHTRERVEQALSGVPAATTLIKPVTPSR
                     LFDAIVRLQHGEMPPPMAAGTLKSDFAEQLRAVRGAQVLLVEDNLVNQQIALAFLSMG
                     GMQVTVANDGLEAVEWVKKASFDLVLMDMQMPGMDGPQATRVIRQLPQGAQLPVIAMT
                     AAAMEEDKQECLAAGMNDHVSKPIDPKELVRALLAWLPPARGAGAMR"
     gene            complement(8431..8643)
                     /locus_tag="VFV25_09110"
     CDS             complement(8431..8643)
                     /locus_tag="VFV25_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393526.1"
                     /translation="MNADTMSQERYELRFESFFNPGRAFAFPCDAQGRVDLNALSRSV
                     LDNYLYARAVIGHEFLTPSVIALVGA"
BASE COUNT         1401 a         2905 c         3026 g         1493 t
ORIGIN      
        1 cctcggtcca gaaggcggca ggcccgagcc aggcgcgcag gatgctcgcg atcatctgcg
       61 tcaccgtggt cttgccgttg ctgccggtga cggcgatcag cggcaggctg aactgcgcgc
      121 gccaggccgc ggcgagctcg cccagcgcct gcttggagtc cgtcacctgc aggccgggca
      181 gaccggcctc ggccagcccg cgctgcgcca gcgccgcgat ggcgccggcc gccttggcct
      241 cgagcaggaa agcgtgcgca tcgaagcgct cgccgacgat ggcgacgaag agatcgcccg
      301 gctgcaggct gcgcgtgtcg ctgtgcacgc gcttgatctg cagcgcgccc gcctgcgtgg
      361 ccccgcccac cagcgtggca ccgggcagca gggcaagggc ctgggccagc gtcatcatca
      421 tgcgggcctc cgggcactca ggacttcctc tgctacctgg acatcggaaa acggcagttt
      481 gacgccgcgg acttcctggt agtcctcatg gcccttgccg gcgatcagca ccacgtcccc
      541 gggcgcggcc tgctgcaagg tccggatgat cgcgacgcgg cgatcctcga tcacctcgac
      601 gccatcgccc cgtgcgacgc cggactggat ctgcgccagg atctgcgcgg gttcctcgtc
      661 gcgcgggttg tcgctggtga tgacgacgcg atcggccgca cgggctgcaa tgccgcccat
      721 cagcggccgc ttggtggcat cgcggttgcc gccgcagccg aacacgcacc acagctggcc
      781 gccgccctgc cgtgccaacg agcgcacggc ctgcagggcc ttgtccagcg catcgggcgt
      841 gtgggcgtaa tcgaccaccg cgaccggcag gcccgcagtg ccgcctaccc gctgcatgcg
      901 cccgggaacc ggcgtgagca aggggcagat gccggccgcg tcggccagcg ggatgtccag
      961 cgcgcgcagg ccgcccagca ccgcgagcag gttgctcgcg ttgtagtcgc cgatcagcgt
     1021 cgttcgcacc tgcacttcct gctcgccttc gcgcagcacg aagctcaggc cgccgtcgcg
     1081 gtagccgagc cggctcgcct gcaggcgggc agggcgcgac agcgaatagg tccagacgtc
     1141 gagcttgctc tccgcggcca ggtccacggc cagcttcgcg ccctgctcgt cgtccacgtt
     1201 gacgacggcg gccttcagcc ccggccaggc gaagagctcg gccttggccg cccagtaggc
     1261 ttccatggtg ccgtgatagt ccaggtggtc gcgcgtgaaa ttggtaaaca gggccgccgc
     1321 gatacgggtg ccggtcaggc gtccttcgac gatgccgatc gacgaggcct cgatggacgc
     1381 cgcctcgtag ccgcggtcga cgaagtcgcg gaaggcggcc tgcagcgtca ccggatccgg
     1441 cgtcgtgagg ccggtgctcc tgacttgcgg cggcacgccc acgcccagcg tgccgaccac
     1501 gcccgcgccg cgccccaagg cattcagcgc ctgcgccacc caccacgagc tggaggtctt
     1561 gccgttggtg ccggtcacgg caacgacgtc gagctcctgg gtggggtcgc cgaaataatg
     1621 ggccgccagc gggccggttg catgcttgag cccgggcagc gcggcgatgc gcgcgtcgtc
     1681 gaagccgaag gcttccacgc cttcggcctc gaccacacag gtggcggcgc cggcggccag
     1741 ggccgcgggc acgtatttgc ggccgtcggt cgcatagccc ggccaggcga tgaaggcgtc
     1801 gcctggctgc acctgccggc tgtcggtgcg cagcgtgccc ttcgaggccc actcgcgcag
     1861 ccagcgcgcg gcggcctgcg gtgatttgag ccgggtcagc atcagaagct ctcctcttcg
     1921 gcctgcatct tctgcgcgac gatctgcggc ttgatctcca gatcgggctg cacgcccatc
     1981 atgcgcaggg tctgctgcac cacctcgctg aacaccgggc ctgcgacctc gccgccgaag
     2041 tgcttgcccg cgctcggctc gtcgaccatc acggcgacga tgatgcgcgg ggcattgatg
     2101 ggggccatgc cgacgaacca ggagcggtac ttcttggtgt tataggtctt tccttcctgc
     2161 ttgtaggccg tgccgctctt gccgcccacc gaatagccga tggtgcgcgc ctgcggcgag
     2221 gtgccgccgt tgtcggtcga catttccagc atgtggcgta tggcgctggc ggtctgcgcc
     2281 gacatgatgc gtgtgccgcc cacgggctgg tcgcgcttgg tgagcgtcac cgggatcagc
     2341 tcgccgtcgc gcgcgaaggc cgtgtaggca tgcgcgatct ggaacagcga gaccgacagg
     2401 ccgtagccat agctcatcgt cgcctgctcg atggggcgcc agctcttgta agggcgcagc
     2461 cggccggtca ccgcgccggg gaagggcagc tgcgggcgct ggccgatgcc ggccgaggcg
     2521 aacatctccc acatctcgcg cggctgcatg cgcatcgcga tcttggcggt accgacgttg
     2581 ctggatttct gtatcacctg ctccaccgtg agggccccgt gcggatgggc gtcgctgatg
     2641 gtggagccgg tgatcgtcat gcgcccgggc gaggtgtcga tcacggtctg cggcgtgacg
     2701 cgcccggttt ccagcgccag cgcgatcgtg aagggcttca tcgtcgagcc cggctcgaag
     2761 gtgtcggtca gcgcgcggtt gcgcagctgc ccgcccgcga ggttctggcg gttgctgggc
     2821 gaatagctcg ggtagttggc cagtgccagc acctcgccgg tctgcgcgtc cagcaccacc
     2881 accgagccgg ccttggcctg gttttccgtc accgcgtcgc ggatgcgctg gtaggcgaag
     2941 aactgcacct tggagtcgat cgacaggtcg atgtcctggc cgtccatcgg cggcgtgctg
     3001 tcgccgatgt cctccagcac gcggcccagg cggtccttga tcacgcggcg cgtgccatcg
     3061 cggcccgcca gctccttctg gaaggccagc tccacgcctt cctggccctt gtcctcgacg
     3121 ttggtgaagc ccaccacatg ggccgcggcc tcgccctcgg gatacttgcg cttgtattcc
     3181 tgcagctggt agacgccctt gatgccgagc tgcttgacct gtgcggccac gctctcgtcg
     3241 acctggcggc gcagccagac gaagttctgg ttctcgccca ggcgcttgtc cagctcggcc
     3301 ggcgtcatgg cgagcagctt cgccagggca cgcagctggg tcttgttggc atccaggtcc
     3361 ttgggcaggg cccagatcga cggcgcgggt acgctggagg cgagcagcaa gccggtgcgg
     3421 tcgatgatgc ggccgcggtt ggccggcagc tccagcgtgc gggcgtagcg gctctcgccc
     3481 tgcttcagat agaagtcggt gcccagcacc tggatgtaga gggcgcgccc caccagcaga
     3541 cagaaaccca ggccgatgcc gaccaccagc agcttggagc gccacggcgg cgtcttggca
     3601 gcgagcagcg ggctggtcgc atagcgcacg ctgcggctcg tcgcgcccgc ctggcgcttg
     3661 ggttcacgct tgaaccagct cattgcgcgg ccctcgcgga agccgcactg ttttgcggca
     3721 ccacgtattc ggtcacggcc ggattagcgg tgcgcatgcc aagtttctcg cgagcgacct
     3781 tctccacgcg cagcggcgtg gcctgggcgc gctgctcgac ctgcagccgc tcgtagtcgg
     3841 tgtccaggcg gtgctcctca accttcgcgc gatccagctc ggcgtagagc cgccgccctt
     3901 cgtaggagac tttcacgaga taaaggctgc tgacgatcag tgcaagcagc agcaggaggt
     3961 tgatacggct catgccgccg ctcccagcgg cgcatcggtg cgctcggcca cgcgcaggat
     4021 ggccgagcgc gcgcgcgggt tctcgctgat ctcggcagcg ctcggcttga tgcgcgccag
     4081 cgccttcagc agcgagggct tgggctcggc aaagggcgcg cggcgatcga cttcgtgctt
     4141 gctgtgccgc gcgatgaagg tcttgacgat gcggtcttcg agcgagtgga agctgatgac
     4201 cacgaggcgg cctttcggcg ccagcgcggc gagggccgcg tttaggcctt gttcgagctc
     4261 ctcaagctcg gcgttgacga gaatccgaag agcctgaaat gtgcgcgttg aaggatcctg
     4321 gcccggttcg cgggtcttga ccgcacgagc cacgacttcg gatagctcag ctgtgctttg
     4381 aacagcacgc ccgctttccc ggcgagccac aagcgccttt gcaatcgata cagcaaaccg
     4441 ttcttcccca tagtcgcgta tcacctccgc aatgcggcgc tcgggagcgc tggccaaaaa
     4501 atccgctgcg ctctggcccc gggtggggtc catacgcata tccagcgggc cgtcgaaacg
     4561 aaagctgaag ccgcgtgccg ggttgtcgat ctgcggcgac gacacgccca ggtcgagcag
     4621 cacgccctgc acggcggcga tgccgcgctc ggccagtgcg cggccgagct ctgcgaagct
     4681 cgcgtgctcg atagcaaaac gcgggtcaga aacccgcgtt ttgccttggc ttgcgtgggc
     4741 gattgcgtct gggtccttgt cgaaggcgat cagccgtcct tctttggaca gcctcgacag
     4801 caccagcctc gagtggccgc cgcggccgta ggtgccgtcg acgtagacgc cgtcgggcga
     4861 gctcacgagc gcgtccaccg cctcgtgcag caggacggtg cggtgctgcc agggacctgc
     4921 gtcaaatgct tccatggtgg ctcagaagct gaagtccttg aggacctcgg gcatgccttg
     4981 ctgcatgacc tcggcctcgt gctcggcatg acgctgcgcg tcccagagct cgaaatggct
     5041 gcccatgcca agcagcatca cgtccttgct gaggcctgcc gcgacgcgca gctcgggcga
     5101 gatcagcacg cgcgaggagg cgtcgatttc cacgtccatc gcgctgccga ggaagatgcg
     5161 cttccagccg ctggccgaca tgggcagcgc ggcgatgcgc tcgcgaaagc tctcccaggc
     5221 ggggcgggga aagatcatca gacagccatc ggggtgcttg gtgacggtga gctggccttc
     5281 ggcgatggct tgcagcatgt cgcgatgccg cgccgggacg gacaaccgcc ccttcgcgtc
     5341 caacgccagg gccgaagccc cttgaaacat gaagttcgcc acaaacaccc accaaagtgc
     5401 actttctccc actgtagcgg aaggcttgca taagggtcaa gccaagatgt aaaaaaaact
     5461 caatggaatc aagcacttag ccgttgtttc gcaaggaaac agaagaaaaa gtctttcaaa
     5521 aataaggact tgcaatgggg tgtggaagtg ttggatgaga tcgctgcagc gtcatccatt
     5581 caaggggggc gtcaggctgg cgttctggga ggcgggaaac agcccaatgg gtctcgaggc
     5641 ggccagtgac ggggaagccg ctctgccgcg ggagacaaca ggaaaggata cgcgccgcag
     5701 ggcacggtct cgcaacaggc cgtaagacct tctcaaggaa ccccggctgc tcccttcgaa
     5761 atcttgcgcc cgctgccgct tacgggcata aagctgaatg ccacgacaag gcccaagaac
     5821 tgtcagggag acgggagtgg ggcaagacga cgaggctgcc gccgcgctcg gcgagcgcga
     5881 cgccgcgcag atggacgatg acaggccggc ctacttgctg agccaggtgg ccgggcgcgc
     5941 tcagcagctg cgtgcggctg tcgtgctgct ggtctgcgcg gcggtcttcg ctgcgctggc
     6001 gcccttcgcc cagctgaagc tggcgccggt ccccgccttc atcccgatgt acgaggccgc
     6061 gctggtggtc accgacgtga tcacggccgt gctgctgttc gggcaatacc gcgtgctgcg
     6121 atcgccggct ttcgcgctgc tcgccagcgg ctacctgttc acagcgctca tggccatgag
     6181 ccatgcgctc acgtttcctg gcctcttcgc ccccgagggg ctgctcgggg cggggcaaca
     6241 gagcacggcc tggatctaca tgttctggca cgccggcttt cccttgttcg tgctgggcta
     6301 tgtgtggctg cgccgcaatg aatcggccat cggggcggcg cgtgtgcgcc tcatcgccgg
     6361 cacgagcccg ttgctgtcag tgcttgtgac gggagggctc gtggcgctgg cgactgccgg
     6421 ggaaacactg ctgccggcca tcatggtcgg caaccactac tcgccgagca tgctgggcac
     6481 ggttgcgagc gtctggctct tcagcctggc agcgctggct gcactttggg cgctgcgctc
     6541 gcgcacggtg ctcgacacct ggctgatcgt cgtcattgtg gtctggctgt ttgatatcgc
     6601 gttgtcggcg atgctcaacg ccggccgctt cgacctcggc ttctatgccg ggcgcagctt
     6661 cgggctgctc gcctgcagcg tggtgctgct ggaactgctg ctggagaacg gcatgctgca
     6721 cgcacggctg gcgcaagcgc acagtgccga gcggctgcgc aaccgggagc tggccatcgc
     6781 gcgcgacgcg gcgcaggccg ctaactcggc caagagcctc ttcctggcca gcatgagcca
     6841 cgagattcgc acgccgatga acgcaatcat cggcctgacc cagttgctgg ccgaaacgaa
     6901 gctggacgac cggcagcacg acttcctggg caaggtgcag acttcgtcgc gggccctgct
     6961 gagcctgctc aacgacatcc tggactactc gaaggtcgag gccggcaaga tcgccctgga
     7021 ggttgaggag ttcagccccg aggagacgat agaaaacgtc ggcaacctgt tcggcgccaa
     7081 gatcgaggag gccgggctcg agctgctgtt caagatcgac gaggccatcc cccagcgcct
     7141 gctgggcgat tcgctgcggc ttgcgcaggt gctgaacaac ctggtcggca atgccatcaa
     7201 gttcacgccg cacggcgaga tcgtgatcgg agccgaagtg atggctcgcg actcgtcgcg
     7261 tgtcgagctg cgcttcagcg tgcgcgacac cggcatcggg ctgacgccgg atcagataca
     7321 gcgcctgttc gaggtgttcg cgcaggctga tcgctcgatc tcgcgaaagt acggcggcac
     7381 aggcttgggc ctggcgatct gcagaaagct ggtcgagctg atgggcggca ggatctgggc
     7441 gcaaagcacg cccggccagg gcagctcctt cttcttcacg gcgtgcttcg agctgcccag
     7501 ccaacgtgcc gagcgcatcg atctgcaccg catccgcggc atgcgcacgc tggtgctcga
     7561 cggacagccg acggcacgcc tgatcctgca gcagacactg cagaactggc gtttccaggt
     7621 cggcacggcc tcgttcgccg acgacgcgtt gcacaagctg cgccgcgcgg aaccgcaagc
     7681 tgcatacgaa ctgctgctgc tcgactggaa ggacgcaggt ctggagctcg tgcacgaggc
     7741 gaggcgcatc acagcagtgc gcactccgac gccgctggcg gtggttgcga tggcgacgct
     7801 gcacacgcgc gagcgggtgg agcaggcctt gagcggcgtg ccggcggcta ccaccctgat
     7861 caagccggtc acgccctcac ggctgttcga cgccatcgtg cggctgcagc acggtgagat
     7921 gccgccgccc atggccgccg gcaccctgaa atccgacttt gccgagcagc tccgcgccgt
     7981 gcgcggcgcg caggtgctgc tggtcgaaga caacctcgtg aaccagcaga tcgcgctggc
     8041 cttcctgtcg atgggcggga tgcaggtcac ggtcgcgaac gatggcctgg aagcggtcga
     8101 atgggtgaag aaggcgtcgt tcgacctcgt gctgatggac atgcagatgc cggggatgga
     8161 cggcccgcag gcgacacggg tgatccgcca gctgccgcag ggcgcgcagc tgccggtcat
     8221 cgccatgacc gccgccgcga tggaagagga caagcaggag tgcttggctg cgggcatgaa
     8281 cgaccatgtc agcaagccca tcgacccgaa ggagctggtg cgcgcgctgc tggcgtggct
     8341 gccgccggcg cgcggcgcgg gcgcaatgcg atgatttgaa gtgtgggatc tcgaggcgga
     8401 taggccgcca ctgcggggca ttcccgaaag ctaggcgccg accagggcga tcactgatgg
     8461 agtgaggaac tcgtggccga tgacggcgcg cgcgtagagg tagttgtcca gcacgctgcg
     8521 gctgagcgcg ttgaggtcga cgcgcccctg ggcatcacag gggaaggcga aggcgcggcc
     8581 agggttgaaa aacgactcga agcgcagctc gtagcgctcc tggctcatcg tgtcggcgtt
     8641 catcgcgtac tccttacacg ccctcaatga cgatcgattg atcacccaga ggcggcagaa
     8701 cttcgaacta cgagccactg tccttgcgag cgccgctgcg agctaaccca ggtatggtcc
     8761 gtgctttgcc gcgggggtcg agcggatcgg acgcctacgg tgttcgcaat attaattcgt
     8821 gcatc
//
LOCUS       DASUCY010000457         3552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_540590, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000457 DASUCY010000000
VERSION     DASUCY010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540590"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="VFV25_09115"
     CDS             <1..755
                     /locus_tag="VFV25_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEY1393527.1"
                     /translation="YLQRLAEAGIPAQATGAHTVVLAQPQPVQVLPGFADGAVSVQDA
                     AAQLAAPLLIGSGASALAPGARVLDACAAPGGKTAHLLELADLDVLALDVDEERLQRV
                     RETLQRLALRAELKAADAGQPETWWDGRPFDAILLDAPCSASGIVRRHPDVRWLRRPT
                     DIAALARTQARLLDALWPLLKPGGRLLYCTCSVFRAEGEQQIDAFLQRETQAIRLPSP
                     GHLLPLPDNSVTAAVPPPDGFFYGLIEKRRDF"
     gene            824..1381
                     /locus_tag="VFV25_09120"
     CDS             824..1381
                     /locus_tag="VFV25_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HEY1393528.1"
                     /translation="MLLACAWLLAGLLLAAPRQARADTVDLPLLEAVQAEDGVLLSFT
                     VRYELSKSLEQALLKGVPLYFVAEAHVYQRRWYWRDKRVGSASRTWRLAYQPLTRRYR
                     IGFSGLSQSYDRLDDAVQAMQQMARWKIADEPRAGDDGYYVEFGYRMDMSQLPRPLQI
                     GFGGEADWSLSVERKINVANEAPAH"
     gene            1444..>3552
                     /locus_tag="VFV25_09125"
     CDS             1444..>3552
                     /locus_tag="VFV25_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEY1393529.1"
                     /translation="MSSNSSIATRWAWIISLVAATGGVMVLSFLLSAATNNRRLYEQH
                     YTWLFWVNVVIAALLAAVILFAALRLATRMMQGKFGSRLLFKLAGIFGLVGLLPGLLI
                     YTVSYQFVSRSIESWFDVKVEGALDAGLNLGRGTLDGMVSDLAAKTRYAAERLADAPD
                     RGEPLALERLREQLSAQSVAVLGSNGQVLSVAGDSVRGGMLVPLTPDRPTSAQLRQVR
                     GGRPIATLEGLDDDTAAGAARGTARIRALAWVPGNALALGSQDRYLLVTQQLPPTLTA
                     NALAVQTAYREYQQRALAREGLRKMYIGTLTLTLVLAVFGAVLLAVALGNQLARPLLL
                     LAEGVSQVARGDLRPTQVFASRDEIGGLTRSFADMTQQLADARAMVERSVAELEAART
                     HLQTILDNLTAGVIVFDHEGLIASVNPGATRILRLPLSVYRVQPLSAVPALAEFAAAV
                     QQRFELHATGPEAGERDHWQDSFELKTAPDRDSLNLLVRGALLPQGARLLVFDDITEV
                     VSAQRSAAWGEVARRLAHEIKNPLTPIQLSAERLQHKLETKLAQPDQQMLAKSVGTIV
                     TQVQAMKQLVNEFRDYARLPAAELKPLDLNALVHEVLILYGAAQESGKLVTECEKGLP
                     PILGDATQLRQIIHNLVQNALDAIAERPDGRVRLITESARNEHGELRAVRLKLVDNGP
                     GFSEKVLKRAFEPYVTTKSKG"
BASE COUNT          552 a         1256 c         1186 g          558 t
ORIGIN      
        1 cctatctgca acgcctcgcc gaggccggca tcccggccca agcgacggga gcgcacacgg
       61 tggtgctggc ccagccccag ccggtgcagg tcttgccggg tttcgccgac ggtgcggtgt
      121 cggttcagga tgcggcggcc cagctggccg cacccctgct gatcggcagc ggggcgagcg
      181 cgctagcgcc cggcgctcgg gtgctcgacg cctgtgccgc gccgggcggc aagacggcgc
      241 atctgctgga gctggccgat ctggatgtgc tggcgctgga cgtcgacgaa gagcgcctgc
      301 agcgtgtgag ggaaacgttg cagcgcttgg cgttgcgggc cgagctcaag gcggccgatg
      361 ccggccaacc cgagacctgg tgggacggcc gccccttcga tgccattctg ctggacgccc
      421 cctgcagcgc ctccggcatc gtgcgccgcc acccggacgt gcgctggctg cggcgaccca
      481 ccgacatagc cgcgctggcc cgcacccaag cccggttgct ggatgcgctg tggccgctgc
      541 tgaagcccgg cgggcggctg ctttactgca cctgctcggt cttcagggcc gagggcgagc
      601 agcagatcga cgcttttttg caacgtgaaa cccaagcgat tcggctgcct tcgcccggtc
      661 acctgctgcc cttgcccgac aattcggtaa cagccgccgt cccacccccg gacggcttct
      721 tttacggctt gatcgagaaa cgccgcgatt tctagctgga acccaagtgc aaagccgccg
      781 ccgcgaggta ccaagcagtg actggccccg cctggcgcgg ggtttgctgc tggcttgtgc
      841 gtggctgctc gccggactgc tgcttgcggc gccgcgccag gcgcgggccg acacggtgga
      901 tctgcccttg ctggaggccg tccaggccga agacggcgtg ctgctgagct tcaccgtgcg
      961 ctatgagttg tccaaatcgc tggagcaggc cctgctgaag ggggtgccgc tgtatttcgt
     1021 ggccgaagcc catgtctacc agcgccgctg gtattggcgc gacaagcggg tcgggtcagc
     1081 cagccgcacc tggcgcctgg cctaccagcc gctgacgcgg cgctaccgca tcggcttcag
     1141 cggcctcagc cagagctatg accgcctcga cgacgccgtg caggccatgc agcaaatggc
     1201 acgctggaag attgccgacg agccccgtgc cggcgatgac ggctactatg tcgaattcgg
     1261 ctaccgcatg gacatgagcc agctgccgcg gccgctgcag atcggcttcg gcggcgaggc
     1321 cgactggagc ctcagcgtcg agcgcaagat caacgtggcg aacgaggcgc ccgcgcactg
     1381 aagctgcggt gccctggcac ccgtgcagtc ccgagcaggc cccgccatcc cgcgacccat
     1441 tttatgagca gcaacagcag cattgccacc cgctgggcct ggatcatctc gctggtggcc
     1501 gccaccggcg gcgtgatggt gctcagcttc ctgctttctg cggccaccaa caaccggcgc
     1561 ctctacgagc agcactacac ttggctgttc tgggtcaacg tcgtgatcgc cgcgctgctg
     1621 gccgccgtga tcctgttcgc cgcgctgcgc ctggccacgc gcatgatgca aggcaagttc
     1681 ggcagccggc tgctgttcaa gctggccggc atcttcgggc tggtcggcct gctgcccggc
     1741 ctgctcatct acacggtgag ctaccagttc gtctcgcgca gcatcgagag ctggttcgac
     1801 gtcaaggtcg aaggcgcgct ggatgccggc ctcaacctcg ggcgcggcac gctggacggc
     1861 atggtcagcg acctcgccgc caagacgcgc tatgcggctg agcgcctcgc cgatgcgccc
     1921 gatcgcggcg agccgctggc gctggagcga ctgcgtgagc agctctcggc ccagagcgtt
     1981 gccgtcctcg gcagcaacgg ccaagtgttg tccgtcgcgg gtgacagcgt gcgcggaggc
     2041 atgctggtcc cgctgacgcc ggaccgcccg acaagcgcgc agctgcgcca ggtgcgcggc
     2101 ggtcgcccca tcgccacgct ggagggcctg gacgacgaca cggccgcagg tgccgcgcgc
     2161 ggcaccgcac gcatacgcgc cttggcctgg gtgccgggca atgccctggc cctcggcagc
     2221 caggatcgct atctgctcgt gacccagcag ctgccgccca cgctcacggc caatgcgctg
     2281 gccgtgcaga ccgcctatcg cgagtaccag cagcgcgcgc tcgcacgcga aggcctgcgc
     2341 aagatgtaca tcggcacgct gacgctgacg ctggtgctgg ccgtgttcgg tgccgtgctg
     2401 ctggcggtgg ctttgggcaa ccagctcgcg cgtccgctgc tgctgctcgc cgaaggcgtg
     2461 agccaggtgg cgcgcggcga cctgcgcccg acccaggtct tcgcctcgcg cgacgagatc
     2521 ggcggcctga cgcgcagctt tgccgacatg acccagcagc tcgccgatgc gcgcgccatg
     2581 gtcgagcgca gcgtcgccga gctggaggcg gcgcgtaccc acctgcagac catcctcgac
     2641 aacctgaccg ccggcgtcat cgtgttcgac cacgagggtc tgattgccag cgtcaatccc
     2701 ggcgccacgc gcatcctcag gctgcctttg tcggtctatc gcgtccagcc gctgagcgcc
     2761 gtgccggccc tcgccgaatt cgctgctgcc gtgcagcagc gcttcgagct gcatgcgacc
     2821 ggccccgagg ccggcgagcg cgaccattgg caggattcct tcgagctcaa gaccgcgccc
     2881 gacagggaca gcctgaacct gctcgtgcgc ggcgccctcc tgccccaggg cgcgcggctg
     2941 ctggtgttcg acgacatcac cgaagtcgtg tcggcccaac gcagcgcggc ctggggcgaa
     3001 gtggcgcgcc gcctggcgca cgaaatcaag aacccgctca cgcccatcca gctgtccgcg
     3061 gagcgcctgc agcacaagct ggagaccaag cttgcgcagc ccgatcagca gatgcttgca
     3121 aagtcggtgg gcaccatcgt cacccaggtg caggcgatga agcagctggt caacgaattc
     3181 cgagattacg cccgcctgcc cgccgccgag ctgaagccgt tggacctgaa cgcgctggtg
     3241 cacgaggtgc tgattctcta tggcgctgcc caggaaagcg ggaagctcgt gaccgaatgc
     3301 gaaaaaggac tgccgcccat cctgggcgat gcgacccagc tgcgacagat catccacaac
     3361 ctggtgcaaa acgcgctcga cgccatcgcc gagcgccccg atggccgcgt acgcctcatc
     3421 accgaatccg cacgcaacga gcacggcgag ctgcgcgccg tgcggctcaa gctcgtcgac
     3481 aatggcccgg gcttctccga aaaagtgctc aagcgtgcct tcgagcccta tgtcacgacc
     3541 aagagcaagg gg
//
LOCUS       DASUCY010000458         5639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_684795, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000458 DASUCY010000000
VERSION     DASUCY010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 5639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5639
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684795"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VFV25_09130"
     CDS             <1..417
                     /locus_tag="VFV25_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY1393530.1"
                     /translation="GRASNVELSAAAHLSAPQCFRRVKALEERGVIRGYAAQVSREAL
                     GYGVVAFVNVDIAAEQFGRVREFEQIIRGFPEILECHTVSGDSDYLLKVVARDLKALS
                     QFLTDRLMQISGVANVRSMICMEEIKPPAPLPTDAA"
     gene            553..1071
                     /locus_tag="VFV25_09135"
     CDS             553..1071
                     /locus_tag="VFV25_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6622 family protein"
                     /protein_id="HEY1393531.1"
                     /translation="MSALTVFSHTPNWVFVLLFALIALGISQAVTRQVTLRRVSLLPL
                     SMAVLSFAGLASVFGAQASVLLPWAAAAAAAAVLVARRAPPPGTRYDAARRVFTLPGS
                     FMPLLLILGIFCTRYAVNAVLAMQPGLAQQAEFVILVGAVYGGFSGSFLGRAARLWRL
                     AAQSGNGALSAV"
     gene            1101..2231
                     /locus_tag="VFV25_09140"
     CDS             1101..2231
                     /locus_tag="VFV25_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY1393532.1"
                     /translation="MTATHAPREFTLPGFWRRGLHTVFICVLIALLLYVFAGGNWWLN
                     LLYSLCIGLNCWLFIDGSRQIIARLARTRHSGDSVRLRHGWLGWARMSFVIVAGATYG
                     YVAGSLLADLLTGRHSPMPLVSSWRGYSLSGFIALTACVAGTYYFSSRQQIAEARTEA
                     ESARRMAAEHQLKLLEAQLEPHMLFNTLANLRALIALDPPRAQEMLDHLIGFLRATLQ
                     GSRVGMHPLRDEFARLADYLALMRIRMGERLQVELQLPEALAQQPVPALLLQPLVENA
                     IKHGLEPHRGGGRIVVSATSEGQELLLCVMDNGAGLSAGAAQPSRGFGMTQVSERLAT
                     LYGERASLALLTASAQSGTLARIRLPLAAGSKVANEISKTCA"
     gene            2228..3055
                     /locus_tag="VFV25_09145"
     CDS             2228..3055
                     /locus_tag="VFV25_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEY1393533.1"
                     /translation="MSSNAPTALIAEDEALLAQALRTELGKQWPQLQVLGLAEDGESA
                     VELALALGPDVLFLDISMPGMTGLQAAQAIIEDWPEGAALPLIVFATAYDQYALQAFE
                     HAAVDYLLKPVQAERLARCVQRLQALLVQRDAKGGKDAATLDAAAVERLRSLLGMPGP
                     AAAVSAPPPLSVIQAGIGATLHMVPVAEVLYFEAADKYVRVVTAQREHLIRLSLRELL
                     PQLPPGRFWQIHRGTVVRNDAIASVTRDEAGKLHLALRERPEQLVVSRMYAHLFKPM"
     gene            complement(3118..3465)
                     /locus_tag="VFV25_09150"
     CDS             complement(3118..3465)
                     /locus_tag="VFV25_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393534.1"
                     /translation="MNRSLKTLALASLVLAGTLASGVARADRVYWSVGVNAPGYPVGV
                     GATFSNAPGVYVAPAPVIYAPPPVYVAPPPVVYAPPVYVAPRPVYYGPAPVVYGGYYR
                     GWKHRHHHDDDDD"
     gene            3623..>5639
                     /locus_tag="VFV25_09155"
     CDS             3623..>5639
                     /locus_tag="VFV25_09155"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HEY1393535.1"
                     /translation="MTASAPKEEHKASNFLRSVVERDLHAGAYAGRKFAGTPGDGTHH
                     EQGRPDPARIRTRFPPEPNGYLHVGHAKSIWLNFSLARDFGGVCHMRFDDTNPEKEEQ
                     EYVDSIVDAVQWLGYSWEANNTSHLYYASNYFDFMYRAAEYLIETGHAYVDEQSAEEM
                     KVNRGDFSTPGIDSPYRYRSRQENLARLREMRAGKHADGAMVLRAKIDMASPNINLRD
                     PALYRIKHAEHHNTGNAWCIYPMYTFAHPIEDALENISHSICTLEFEDQRPFYDWLLE
                     RIVPILRAPQFEHAKRLIEGIAAAGMEAGKEFALVCHNHADKLFASDAEGEMRALFAR
                     WEHDREAVLRDLPAFFALLRRDTAQFAPLLAHALDEHEVNVFKLPHQYEFARLNLTYV
                     ITSKRKLKALVDEGIVDGWDDPRMPTIVGLRRRGYTPEAIQLFCERSGVSKAGGWIDY
                     GSLDAALREDLEGKAARAMAVLDPVKLKLVNWTDVFGGEAHREPCTAPAHPARPELGA
                     REFSLGPEVWIEREDFSEVPPKGFFRLFPGNKVRLKYGYVVECTGCEKDADGRITAVL
                     ANVVPDTKSGTPGADAVKVKGTITWVGVHDGLQAELRLYDRLFTEAQPDAGGRDFMAA
                     LNPRSKQVVTGYLEPSLAKAEADERFQFERHGYFVADRKDHKLGRPVI"
BASE COUNT          963 a         1949 c         1790 g          937 t
ORIGIN      
        1 ggccgtgcct ccaatgtgga gctctcggct gcagcgcatc tgtcggcacc gcaatgcttc
       61 cggcgcgtca aggctctgga agaacggggc gtgatccgtg gctacgcagc ccaggtcagc
      121 cgcgaggcgc tgggctacgg cgtggtggcc ttcgtcaacg tggacatcgc cgccgagcag
      181 ttcggccgcg tgcgcgagtt cgagcagatc atccgcggct tccccgagat cctggagtgc
      241 cacaccgttt cgggcgactc ggactatctg ctcaaggtcg tggcccgcga tctcaaggcg
      301 ctgtcgcagt tcctcaccga ccggctgatg cagatctcgg gcgtggccaa tgtgcgctcg
      361 atgatctgca tggaggagat caagccgccg gcaccgctgc cgacggatgc ggcctagctg
      421 ttagcagtag gcccgcggcg tttcgcgccg cggtgccagc gtttcgcgac ggccatgggc
      481 gcctcgtcgc gccgcgctgg ctcccgcagg gtgcaatgcc caacatgact gcgtcccaac
      541 caggagatcg tcatgtctgc cctcaccgtc ttcagccaca cgccgaactg ggtcttcgta
      601 ctcctctttg cgctgattgc actcggcatc tcgcaagccg tcacgcggca agtcacgctg
      661 cgccgcgtat ccctgctgcc cctgagcatg gcggtgctgt cattcgccgg cctcgcttcg
      721 gtcttcggcg cccaggctag tgtgctgctg ccctgggctg cagccgcggc ggcggcggcc
      781 gtactggtcg ccaggcgggc accgccccct ggcacccgct atgacgccgc acgcagagtc
      841 ttcacgctgc ctggcagctt catgccgctg cttctcatcc tggggatctt ctgcacccgc
      901 tatgccgtta acgcagtctt ggcgatgcag cccggtctcg cgcaacaagc cgaattcgtc
      961 atcctcgtcg gcgccgtcta cggcggcttc agcggcagct ttctcggccg cgcggcgcgg
     1021 ctatggcgcc tggctgccca aagcggaaac ggcgctcttt ctgcggtctg agcctggcgc
     1081 atcgtccaca atgactgcag atgacagcga cccatgcccc gcgcgaattc acgctgcccg
     1141 gcttctggcg ccgcgggctg cacacggtct tcatctgcgt gctgatcgcg ctgctgcttt
     1201 acgtcttcgc cggtggcaac tggtggctca acctgctgta ttcgctgtgc atcggcctga
     1261 attgctggct cttcatcgac ggcagccggc agatcattgc caggttggct cgcactcgac
     1321 actccggtga cagcgtgcgc ctgcgccacg gctggctggg ctgggcccgg atgtcgttcg
     1381 tgatcgtcgc tggcgccacc tacggctacg tcgcaggcag cctgctggcc gatctgctca
     1441 ccggccgcca ttcgccgatg ccgctggtgt cgagttggcg cggctattcc ttgtcgggct
     1501 tcattgcgct gacggcctgc gtcgccggca cctattactt cagctcccgc cagcagatcg
     1561 ccgaagcccg caccgaggcc gaaagcgcgc gccgcatggc ggccgagcat cagctcaagc
     1621 tgctggaagc ccaactcgaa ccgcacatgc tgttcaacac actggccaat ctgcgcgcgc
     1681 tcatcgccct ggacccaccg cgcgcccagg agatgctgga ccacctgatc ggcttcctgc
     1741 gcgccacgct gcagggcagc cgcgtcggga tgcatccgct gagggacgaa ttcgctcgcc
     1801 tagccgacta ccttgcgctg atgcggatcc gcatgggcga gcgcctgcag gtcgagctgc
     1861 aactgcccga agcgctggcg cagcagccgg tccccgccct gctgctccag ccgctggtgg
     1921 aaaacgccat caagcacggc ctggaaccgc accgcggcgg cgggcgtatc gtcgtcagtg
     1981 cgacgagcga ggggcaggag ctgctgctgt gcgtgatgga caacggtgcc ggcttgtcgg
     2041 caggcgccgc acaacccagc cgtggcttcg gaatgactca ggtgagcgag cggctggcca
     2101 ccctctacgg cgagcgtgcc agcctcgcgc tgctaacggc ctcggcacag agcggcacct
     2161 tggcgcggat caggctgccg ctggccgccg gctccaaagt cgccaacgaa atcagtaaga
     2221 cctgcgcatg agcagcaacg cccccaccgc gctgattgcc gaagacgaag ccctgctggc
     2281 ccaagccttg cgcaccgagc tgggcaagca atggccgcaa ttgcaggtgc tgggactggc
     2341 ggaggatggt gaatccgccg tcgagctggc gctggcgctc gggcctgatg tgctgtttct
     2401 ggacatcagc atgcccggca tgactgggct gcaagcggcc caggccatca tcgaagactg
     2461 gccggagggc gccgcgctgc cgctcatcgt cttcgccacc gcctacgacc agtatgcgct
     2521 gcaggccttc gagcatgcgg ccgtggacta tctgctcaag cccgtgcaag ccgagcggct
     2581 cgcgcgctgc gtgcagcggc tgcaagcgct gctggttcag cgtgatgcga aaggcggcaa
     2641 ggatgctgcg actctcgacg cggccgccgt cgagcgactg cgcagcctgc ttggcatgcc
     2701 cggaccggct gcggctgtgt cagcgccgcc accgctcagc gtgatccaag ccggcatagg
     2761 cgcgacgctg cacatggtgc cggtggccga ggtcttgtac ttcgaggcag ccgacaaata
     2821 cgtgcgcgtc gtcaccgccc aacgcgagca cctgatacgg ctctcgctgc gcgagctgct
     2881 gcctcagttg ccgcccgggc gtttctggca aatccatcgc ggcaccgtgg tgcgcaacga
     2941 cgcaatcgcc agcgtcaccc gcgacgaggc cggcaagctg catctcgcgc tacgcgagcg
     3001 gcccgagcag ctagtcgtga gccgcatgta tgcacatctc ttcaagccga tgtgagggcc
     3061 caaaaagcat cgaggcgccg cgaggcgcct cgtgctgatg agagaagcag gctgcgatca
     3121 atcgtcgtca tcgtcatgat gatggcgatg cttccagccg cggtagtagc cgccgtagac
     3181 cactggcgcc ggaccgtagt agacggggcg cggcgccacg tagaccggag gtgcgtagac
     3241 caccgggggc ggcgcgacat agacgggcgg cggcgcgtag atcaccggtg ccggcgcgac
     3301 atagacgccg ggcgcattgg agaaggtcgc ccccacgccc accggataac ccggcgcatt
     3361 gacacccacc gaccaataga cgcggtccgc acgggccacg cccgaggcca gcgtgccggc
     3421 cagcaccagc gaagccaggg ccagagtctt gagggaacgg ttcatgtctt tctccttctg
     3481 cagtccaccc tcgctgtgcg cgatttcggg tggttcatcc atagaacgct tgagcattgc
     3541 atcggttcac cgcggagttg tgaacagtcg taagggactg cggcctctat agaatgactc
     3601 aatcacccga acatccccct tcatgactgc ttctgccccg aaagaagagc acaaagcgag
     3661 caatttcctg cgctcggtgg tcgaacgcga cctccatgcc ggcgcctacg cgggccgcaa
     3721 attcgccggc acgccgggcg acggcaccca ccatgagcaa ggccgccccg acccggcacg
     3781 catccgcacc cgcttcccgc ccgagcccaa tggctatctg catgtgggcc acgccaagag
     3841 catctggctc aacttcagcc tggcgcgcga cttcggtggc gtctgccaca tgcgcttcga
     3901 cgacaccaat ccggaaaagg aagaacagga gtacgtcgat tccatcgtcg atgccgtgca
     3961 atggctcggc tacagctggg aagccaataa cacttctcac ctttattacg cgagcaacta
     4021 tttcgatttc atgtaccgcg cggccgagta cctgatcgaa accggccacg cctacgtgga
     4081 cgagcagagc gccgaggaaa tgaaggtgaa tcgcggcgat ttctcgacgc ccggcatcga
     4141 cagcccctat cgctaccgct cgcgccaaga aaacctcgcg cgcttgcgcg agatgcgcgc
     4201 gggcaagcac gcggacggcg cgatggtgct gcgcgccaag atcgacatgg cctcgcccaa
     4261 catcaatctg cgcgacccgg ccctgtaccg catcaagcac gccgagcatc acaacaccgg
     4321 caatgcctgg tgcatctatc cgatgtacac cttcgcgcac ccgatcgagg atgcgctgga
     4381 gaacatcagc cacagcatct gcaccctcga gttcgaggac cagcgcccct tctacgactg
     4441 gctgctggag cgcatcgtgc ccatcctgcg cgcgcctcaa ttcgagcacg ccaagcgcct
     4501 gatcgaaggc atcgcggccg ccggcatgga ggccggcaag gaattcgcgc tggtctgcca
     4561 caaccatgcg gacaagctct tcgccagcga tgccgaaggc gaaatgcgcg ccctcttcgc
     4621 gcgctgggag cacgaccgcg aggccgtgct gcgcgacctg cctgcctttt tcgcgctgct
     4681 caggcgcgac acggcgcagt tcgctccgct cttggcccat gcgctggacg agcacgaagt
     4741 caacgtcttc aagctgccgc accagtacga gttcgcacgc ctcaacctca cctatgtcat
     4801 caccagcaag cgcaagctga aggcgctggt ggacgaaggc atcgtcgatg gctgggacga
     4861 cccgcgcatg cccaccatcg tcggcctgcg ccgccgcggc tacacgcccg aggcgatcca
     4921 gctgttctgc gagcgcagcg gcgtcagcaa ggccggcggc tggatcgact acggcagcct
     4981 cgatgccgct ctgcgggagg acctcgaagg caaggccgcg cgcgcgatgg ccgtgctcga
     5041 tccggtcaag ctcaagctcg tcaactggac cgacgtgttc ggcggcgaag cgcaccgcga
     5101 gccctgcacg gctcccgccc acccggcccg ccccgagctg ggtgctcgcg agttcagcct
     5161 cggcccggag gtgtggatcg agcgggaaga cttttccgaa gtgccgccca agggcttctt
     5221 ccgcctcttc cccggcaaca aggtgaggct gaagtacggc tatgtggtcg agtgcaccgg
     5281 ctgcgagaag gatgccgacg ggcgcatcac ggccgtgctc gccaatgtcg tgcccgacac
     5341 caagagcggc acgcccggcg ccgatgccgt caaggtcaag ggcaccatca cctgggtcgg
     5401 cgtgcacgac ggcctgcagg ccgagctgcg gctctacgac cggctcttca ccgaggccca
     5461 gcccgatgcc ggcgggcgcg acttcatggc ggcgctcaac ccccgcagca agcaggtcgt
     5521 gaccggctac ctggagccgt cattggccaa ggccgaggcg gacgagaggt tccagttcga
     5581 gcggcacggc tatttcgtcg cggatcgcaa ggaccacaag ctggggcggc ctgtgatca
//
LOCUS       DASUCY010000459         2238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_684802, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000459 DASUCY010000000
VERSION     DASUCY010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684802"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1203
                     /gene="dxs"
                     /locus_tag="VFV25_09160"
     CDS             <1..1203
                     /gene="dxs"
                     /locus_tag="VFV25_09160"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEY1393536.1"
                     /translation="AKGMVVPGTIFEEFGFHYVGPIDGHDLDSLIPTLENMRDKPGAQ
                     FLHVVTKKGYGYKLAEADPVNYHGPGKFDPAVGLQKPATPPKPSFTQIFGQWLCDMAA
                     ADQRLVGVTPAMREGSGMVEFEKRFPERYHDVGIAEQHAVTFAAGLACEGLKPVVAIY
                     STFLQRAYDQLIHDVAIQNLPVVFALDRAGIVGADGATHTGAYDIAYLRCIPNMGVLT
                     PADENECRQALTTAFRQNHPVAVRYPRGSGVGVAIQPELSELPWGKGELRRRGHRVAI
                     LAFGSLLYPALQAAEKLDATVANMRFAKPLDVELIKELARTHEAFVTVEEGCVMGGAG
                     SAVAEALQAAGLNTPVLMLGLADEFVEHGDPAKILSLSGLDAAGIEQSVLKRFGGKLE
                     LVRPAVNG"
     gene            1299..2105
                     /gene="folE2"
                     /locus_tag="VFV25_09165"
     CDS             1299..2105
                     /gene="folE2"
                     /locus_tag="VFV25_09165"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006414537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HEY1393537.1"
                     /translation="MNDLTHPLTSALHIPDTQSERDERHLAIQRVGVRELRYPLQVRI
                     GDAVSPSVATWSLDVALPAEQKGTHMSRFVAWLDALQRPLDAAALREEMAAMLERLHA
                     SEGRIEARFPFFLRKQAPVSGVSSLMDYQGALIAEARGGKTHVWAEVVVPVKSLCPCS
                     KEISDYGAHNQRSHVTIRAELKADTLCWSELVRFAEESASSEIWGLLKRPDEKWITER
                     AYENPKFVEDLVRDVALRLNAEPRIGRYRVTVENFESIHNHSAFAVIDRE"
BASE COUNT          375 a          773 c          762 g          328 t
ORIGIN      
        1 gccaagggca tggtcgtgcc cggcaccatc ttcgaggaat tcggcttcca ctacgtcggc
       61 cccatcgacg gccatgacct cgactcgctg atccccacgc tggagaacat gcgcgacaag
      121 ccgggcgcgc agttcctgca cgtggtgacc aagaagggct atggctacaa gctggccgag
      181 gccgatccgg tcaactacca cggccccggc aagttcgacc cggcggtggg cctgcagaag
      241 ccggccacgc cgcccaagcc cagcttcacg cagatcttcg gccaatggct gtgcgacatg
      301 gccgcggcag accagcgcct ggtgggcgtc acgccggcca tgcgcgaggg ttcgggcatg
      361 gtcgagttcg agaagcgctt ccccgagcgc tatcacgacg tcggcatcgc cgagcagcat
      421 gccgtcacct tcgcggccgg cctggcctgc gagggcctga agccggtggt ggcgatctac
      481 tcgaccttcc tgcagcgcgc ctacgaccag ctgatccacg acgtggcgat ccagaacctg
      541 ccggtggtgt tcgcgctgga ccgcgccggc atcgtgggcg cggatggcgc cacgcacacc
      601 ggcgcctacg acatcgccta cctgcgctgc attcccaaca tgggcgtgct cacgccggcc
      661 gacgagaacg aatgccgcca ggcgctgacc acggccttcc gccagaacca tccggtggcc
      721 gtgcgctatc cgcgcggcag cggcgtgggg gtcgcgatcc agcccgagct gagcgagctg
      781 ccctggggca agggcgagct gcgccgccgc ggccatcgcg tggcgatcct ggccttcggc
      841 agcttgctct atcccgcgct gcaggcggcc gagaagctcg atgcgacggt agccaacatg
      901 cgttttgcaa aacccctgga cgtggagctg atcaaggaac tcgcgcgcac tcacgaggcc
      961 ttcgtcacgg tcgaggaggg ctgcgtgatg ggcggcgccg gcagcgccgt ggccgaggcc
     1021 ctgcaggccg cgggcttgaa cacgccggtg ctgatgctgg gcctggccga cgagttcgtc
     1081 gagcatggcg acccggccaa gatcctgagc ctcagcggcc tggacgcggc cggcatcgag
     1141 cagtccgtgc tcaagcgctt cggcggcaag cttgagctgg tacgccctgc cgtgaacggc
     1201 tgagccgtgc cgataggccc tgtccgacgc ggggagagcc gaaagctgtc atgcttcgcg
     1261 cagcgcgtgc cgacctcggc cgcaaaacga tctcgccgat gaacgaccta acccatcctc
     1321 tgacctccgc cctgcacatt cccgacacgc aaagcgagcg ggatgagcgc cacctggcga
     1381 tccagcgcgt gggcgtgcgc gagctgcgct atccgctgca ggtgcgcatc ggcgacgcgg
     1441 tgtcgcccag cgtggcgacc tggtcgctcg acgtggccct gccggccgag cagaagggta
     1501 cgcacatgtc gcgcttcgtg gcctggctcg acgcgctgca gcgcccgctg gacgccgccg
     1561 cgctgcgcga ggagatggcc gcgatgctgg agcggctgca cgccagcgaa ggccgcatcg
     1621 aggcgcgctt ccccttcttc ctgcgcaagc aggcgccggt gtccggcgtg agcagcctga
     1681 tggactacca gggcgccttg atcgccgagg cacgcggcgg caagacccac gtctgggccg
     1741 aggtcgtggt gccggtcaag agcctgtgcc cctgctcgaa ggagatctcg gactatgggg
     1801 cgcacaacca gcgttcgcac gtcacgatac gcgccgagct caaggccgat acgttgtgct
     1861 ggagcgagct cgtgcgcttc gcggaagaga gcgcctccag cgagatctgg ggcctgctca
     1921 agcgccccga cgagaagtgg atcaccgagc gagcctacga gaatcccaag ttcgtcgagg
     1981 acctggtgcg tgacgtcgcg ctgcggctga acgccgaacc gcgcatcggc cgctaccgcg
     2041 tcaccgtgga aaacttcgag tcgatacaca accactcggc attcgcggtc atcgaccgcg
     2101 aatgagctta gccgcaggcc aagcgggcgc gtagcgctca cgaaggctgg acggtacccc
     2161 cggcacaacc actcggcatt cgcggtcatc gggcgcgaat aggctcggcc gcgggccggg
     2221 gcgcaaagcc gctcttca
//
LOCUS       DASUCY010000460         3657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_154959, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000460 DASUCY010000000
VERSION     DASUCY010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154959"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VFV25_09170"
     CDS             <1..495
                     /locus_tag="VFV25_09170"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY1393538.1"
                     /translation="RELRGVMAHELAHVRHRDILISTVSATMAGAISMLANFAMFFGG
                     RDESGRPANPLAGLLVAILAPLAASLIQMAISRAREFEADRGGAEISGDPAALASALQ
                     KIHHYAAGIPLLPAEKHPETAQMMIMNPLSGGGLRGLFSTHPSTEERVERLMAMARSS
                     VPSR"
     gene            637..1425
                     /locus_tag="VFV25_09175"
     CDS             637..1425
                     /locus_tag="VFV25_09175"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HEY1393539.1"
                     /translation="MSTTASSSLLGGCLRGFILGLALAGLLPAARAGTWNWNDGGGAR
                     VTGSGHVVSENRSLSGYEALALKAPFKLELRQSGHEGLSLSGDDNLLPLVETRVIEGH
                     DGLKTLEIGLKPGSSITSRNEIRVTVDFAKLRALSSEGSGDISGQGLRLGAFSASLSG
                     SGDLSLKDLRADSLKLSIAGSGDFSASGQVPSFSASIAGSGDVHAAELACDDVTVNIA
                     GSGDADVQARKTLNVSIAGSGDVYYRGAARVSQSIFGSGGVHAR"
     gene            1470..2213
                     /locus_tag="VFV25_09180"
     CDS             1470..2213
                     /locus_tag="VFV25_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEY1393540.1"
                     /translation="MSASVASSLRRLACHPEFSHGAKEMAPVSLGLGAWGLVTGVAMI
                     KSGLSVPLALLMSLTVFAGSSQLAALPLLAVGAPLWVIWATALCVNLRFVIFSVQWRP
                     YLMRYPRWKRLVIGYFAADMNYVVFMRRYPDPAKTDGAMAYYAGGVAINWLSWQLPSI
                     TGIVLANAIPTQWGLGFAGTLALLGLTCSLLSDRTTLLSALVAGASAVAAFALPFRLN
                     IVVAIAAAVCAGLLLDAGWRRTAVSGSSS"
     gene            2210..2542
                     /locus_tag="VFV25_09185"
     CDS             2210..2542
                     /locus_tag="VFV25_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEY1393541.1"
                     /translation="MSEGLQATIAILGMAAITVLTRSFFFISDREWRLPPWLQRGLRY
                     APLAALAAVVVPEILMSQGSLIDTWRDARLYSAAAATAYFFWRRGILGTIVVGMAVLL
                     PLRLLLGW"
     gene            2611..>3657
                     /locus_tag="VFV25_09190"
     CDS             2611..>3657
                     /locus_tag="VFV25_09190"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEY1393542.1"
                     /translation="MNVLRFSDLVAQGKVAGQRVFIRADLNVPQDDAGRITEDTRIRA
                     SVPCIEMALKAGAAVMVTSHLGRPTEGEFKPEDTLAPVAQRLGELLGREVPLKSNWVD
                     GVDVKPGQVVLLENCRLNKGEKKNDESLAKKLAALTDIFVHDAFGTAHRAEASTYGIA
                     QFAKIACAGPLLAAEIDAITKALANPKRPLAAIVAGSKVSTKLTILQSLAKNVDQLVV
                     GGGIANTFMLAAGLPIGKSLAEPDLVGEARAVIEAMKVRGAAVPIPVDVVVAKEFKPD
                     AQATVKAATEVADDDLILDIGPKTAAQLAEQLKAAGTIVWNGPVGVFEFDAFAHGTET
                     IARAVAASSAFSIAG"
BASE COUNT          577 a         1289 c         1188 g          603 t
ORIGIN      
        1 cgcgagctgc gcggcgtgat ggcccatgag ctggcccacg tgcggcaccg cgacatcctg
       61 atctccacgg tcagcgcgac catggccggc gcgatctcga tgctggccaa tttcgcgatg
      121 ttcttcggtg gccgcgacga gtcaggccgg ccggccaacc cgctcgccgg cctgctcgtg
      181 gccatcctgg cgccgctggc agccagcctg atccagatgg ccatcagccg cgcccgcgaa
      241 ttcgaagccg accgcggcgg cgccgagatt tccggcgacc cggccgcgct cgcctcggcg
      301 ctacagaaaa tacatcacta tgcggcagga atccctctgc taccggcaga aaagcatcca
      361 gaaaccgcgc agatgatgat catgaacccg ctgtccggcg gtgggctgcg cgggctgttc
      421 agcacccacc cttcgaccga ggaaagggtc gagcggctga tggccatggc acgctcgagc
      481 gtgccatcgc gatagcccca cgctcaggcg tgccagcctg agcaccagct cccgcggcat
      541 tccatcccct tcgcttcgca attcatcgcg cgacggctga cgcctctgcg cgaagcgccc
      601 aacatggcgc ccacacaacg aattggaggc gacacgatgt cgaccactgc atcctcatcc
      661 cttctcggcg gctgcctgcg cggctttatc ctgggcctgg cccttgccgg cctgctccca
      721 gcggcccggg ccggcacctg gaactggaac gacggcggcg gggcccgcgt gaccggctcc
      781 ggccatgtcg tcagcgagaa ccgcagcctg agcggctacg aggccctcgc gctgaaggcg
      841 cctttcaagc tcgaactgcg ccagagcggc cacgaaggcc tgagcctgag tggcgacgac
      901 aacctgctgc cgctggtcga gacccgtgta atcgagggcc acgacggcct caagaccctg
      961 gagatcggcc tgaagcccgg cagcagcatc accagccgca acgagatccg cgtcacggtg
     1021 gacttcgcca agctgcgcgc cttgtcgtcg gagggctcgg gcgacatcag cggccagggc
     1081 ctcaggctgg gagccttcag tgcctcgctt tccggatcgg gcgacttgag cctcaaggac
     1141 ttgcgggccg acagcctcaa gctcagcatc gccggcagcg gcgatttcag cgcaagcggg
     1201 caggtcccca gcttcagcgc cagcatcgca ggcagcggcg acgtgcacgc ggcagaactg
     1261 gcctgcgacg atgtgacggt gaacatcgcc ggcagcggtg acgccgatgt gcaggcccgc
     1321 aagacgctga atgtctccat cgccgggagc ggcgatgtct actatcgcgg tgccgcgcgc
     1381 gtcagccagt ccatcttcgg cagcggaggc gtgcacgccc gctgagcgag cgtccgtgcc
     1441 ccgtagggcg gaatgggaca atcgcgccca tgtccgcctc tgtcgcctcg tcgctgcgcc
     1501 gactcgcctg tcaccctgaa ttcagccatg gcgccaagga aatggcgccg gtttcgctgg
     1561 ggctgggagc ctggggcctg gtcaccggtg tcgcgatgat caagagcggc ctgtcggtac
     1621 cgctcgcgct gctgatgtcc ttgaccgtct tcgccggcag ctcgcagctt gccgcgctgc
     1681 ccctgctggc cgtcggcgca ccgctgtggg tgatctgggc caccgccttg tgcgtcaacc
     1741 tgcgcttcgt gatcttcagc gtgcagtggc gcccgtatct gatgcgatac ccgcgctgga
     1801 agcggctcgt gatcggctac ttcgcggccg acatgaacta tgtggtcttc atgcgccgct
     1861 accccgatcc ggccaagacc gatggggcga tggcctatta cgccggtggc gtcgcgatca
     1921 actggctgtc ctggcagctg ccgtccatta ccggcatcgt ccttgccaac gccatcccca
     1981 cgcaatgggg cctgggcttc gcgggcacgc tggccttgct ggggctgacc tgctcgctgc
     2041 tatccgaccg cacgacgctg ctctcggccc tggtggcggg cgcttcggcg gtggcggcct
     2101 tcgcgctgcc cttcaggctc aacatcgtcg tcgccatcgc cgcagcggtc tgcgccggac
     2161 tcctgctcga cgcgggctgg cgccgcacgg cagtgtctgg gagttcgtcg tgagcgaagg
     2221 cctgcaggcg acgatcgcca tcctcggcat ggccgccatc acggtactga cgcgcagctt
     2281 cttcttcatt tccgatcgcg aatggcgctt gccgccctgg ctgcaacgcg gcctgcgcta
     2341 tgcgccgctg gcggcgctgg cggcggtggt ggtgcccgag atcctgatga gccagggcag
     2401 cctgatcgat acctggcgcg atgcacggct gtactcggcg gcggcggcca cggcgtattt
     2461 cttttggcgc cgcggcatcc tcggcaccat cgtcgtcggc atggcggtgc tgctgccgct
     2521 gcggctgctg ctcggctggt gaagcgatag cgatgacgcc atcctgtaac ttggttactt
     2581 tagaattcca accctagttt catcccgatc atgaacgttc ttcgcttctc cgatctcgtc
     2641 gcccagggca aagtcgccgg ccagcgcgtt ttcatccgcg ccgacctcaa cgtaccgcaa
     2701 gatgacgccg gccgcatcac cgaagacacc cgcatccgcg ccagcgtgcc ctgcatcgaa
     2761 atggccctca aggccggtgc cgccgtgatg gtgacctcgc atctgggccg ccccaccgag
     2821 ggcgagttca agcccgagga tacgctggca ccggtcgccc agcgcctcgg tgaactgctc
     2881 ggccgcgagg tgcccttgaa gagcaactgg gtcgatggcg tcgacgtcaa gcccggccag
     2941 gtcgtgctgc tggagaactg ccgcctgaac aagggcgaga agaagaacga cgaatcgctc
     3001 gcgaagaagc tcgccgcgct gaccgacatc ttcgtgcacg acgccttcgg caccgcgcat
     3061 cgcgccgagg cctcgaccta cggcatcgcg cagttcgcca agattgcctg cgccggcccg
     3121 ctgctggccg ccgagatcga cgcgatcacg aaggcgctgg ccaatcccaa acggccgctg
     3181 gcggccatcg tcgccggctc caaggtcagc accaagctga ccatcctgca gagcctggcg
     3241 aagaacgtgg accagctcgt cgtcggcggc ggcatcgcca acaccttcat gctggccgcc
     3301 ggcctgccca tcggcaagtc gctggccgag cccgacctgg tcggcgaggc cagggccgtg
     3361 atcgaggcga tgaaggtacg cggcgccgcg gtgccgattc ccgtcgatgt ggtggtcgcc
     3421 aaggagttca agcccgatgc gcaggccacc gtgaaggccg cgaccgaggt ggccgacgac
     3481 gacctcatcc tcgacatcgg accgaagacc gccgcacagc tggccgagca gctcaaggcg
     3541 gccggcacca tcgtgtggaa cggcccggtg ggcgtgttcg agttcgacgc cttcgcccat
     3601 ggcaccgaga ccatcgcgcg cgccgtcgcc gcgtcgagcg ccttcagcat cgccggc
//
LOCUS       DASUCY010000461         2126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_974009, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000461 DASUCY010000000
VERSION     DASUCY010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974009"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /gene="gspM"
                     /locus_tag="VFV25_09195"
     CDS             <1..216
                     /gene="gspM"
                     /locus_tag="VFV25_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HEY1393543.1"
                     /translation="SATERLGQAARLAVQGDRATLTLNGVSGDALVAWLDGARHAARA
                     RPIEAQLARSGTGYSGTLVLALAVDKG"
     gene            218..1135
                     /gene="gspN"
                     /locus_tag="VFV25_09200"
     CDS             218..1135
                     /gene="gspN"
                     /locus_tag="VFV25_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HEY1393544.1"
                     /translation="MARRSLFSLSRLPAKRRGFAARTTRWGESTLEVARWARSRKASG
                     RWALGGAVLGLLLGVVLFAPAAWLSNAVASASGQHLLLADARGTVWSGSAVVVLAGGP
                     GSRDARSLPGRMEWSLRPVLIGGPGLLLLAKQACCLNGEVGLRLQPGLGRLRATLLSP
                     PAWTGQWPNAWLGGFGTPLNTLQLGGALRLSSPGLMLEWVQGRLRVDGRADIDLSDVS
                     SRLTTLPRLGSYRLTLSGDPAQEGAAQLQLSTLDGALQLSGNGQFGGPHGVRFQGEAR
                     ADAADEASLNNLLNIIGRRDGARSVITIG"
     gene            1138..>2126
                     /locus_tag="VFV25_09205"
     CDS             1138..>2126
                     /locus_tag="VFV25_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HEY1393545.1"
                     /translation="MSPLRSHPALLRALVAALALSLAAPAPLWAQKGREPVTLNFVNA
                     DIEGVARAMGAILDRQIVVDPRVKGNITLYSEQPMPPSQAFLSFQTALRGLGFAVVEV
                     EGILKVVPEADAKLQTGTVSVGAVNRKGDQIITQIFRLQHENANNLVAVLRPLISPNN
                     TINVNPGNNSLVITDYADNLQRIAKIIAATDIPSATDLEVIPLKHAIASDMATLVQRL
                     SDGGGAGAQAVQGAAGGTLTVMADARSNSLLVRAPNAARLAMVKTVVEKLDQPGQSGA
                     GGSNIYVVYLKNADATRLAQVLRAAYSATGSGGSAAGGASALGGAQGANYPAT"
BASE COUNT          309 a          764 c          760 g          293 t
ORIGIN      
        1 tccgccaccg aacgcctggg gcaagcggcc cgcctcgccg tgcagggcga ccgcgcgacg
       61 ctcacgctca atggcgtgag cggcgatgcg ctggtcgcct ggctcgacgg ggcgcgccac
      121 gccgccaggg cgcggcccat cgaggcccag ctcgcgcgca gcggaacagg ctacagcggc
      181 acgctggtgc tggcactggc ggtcgacaaa ggctgacatg gccaggcgtt cgctcttctc
      241 tttgtcgcgc ctgccggcca agcggcgcgg ctttgctgcg cgcaccacgc gctggggcga
      301 atcgaccctg gaagtggcgc gttgggcgcg ttcgcgcaag gccagcgggc gctgggcgct
      361 gggcggcgct gtgctgggct tgctgctggg cgtggtgctc ttcgcccccg cggcctggct
      421 gagcaatgcc gtggcaagcg ccagcggcca gcatctgctg ctggccgatg cgcgcggcac
      481 ggtctggtcg ggcagcgccg tcgtcgtgct ggcgggcgga ccgggcagcc gcgacgcgcg
      541 ctcgctgccg ggacgcatgg aatggtcgct gcgcccggtg ctgatcggcg gccccggcct
      601 gctgctgctg gccaagcagg cctgctgcct caacggcgag gtgggcctgc gcctgcagcc
      661 cggcctcggc cgcttgcgcg ccacgctgct gtccccgccg gcctggaccg ggcaatggcc
      721 gaatgcctgg ctgggcggct tcggcacgcc tttgaacacc ttgcagctgg gcggcgcgct
      781 gcggctgtcc tcgcccgggc tgatgctcga atgggtgcaa gggcgcctgc gcgtggacgg
      841 ccgtgccgac atcgacctga gcgacgtatc ctcgcgcctg accaccctgc cccgcctggg
      901 cagctaccgg ttgacgctgt cgggcgaccc ggcccaggag ggcgccgcgc aactgcagct
      961 cagcaccctg gacggcgcgc tgcagctgtc cggcaacggg cagttcggcg gcccccacgg
     1021 cgtgcggttc cagggcgagg cgagggccga tgcggccgac gaggcctcgc tgaacaactt
     1081 gctgaacatc atcggacggc gcgacggcgc caggtccgtc ataacgatcg gatgatcatg
     1141 agtcctctga gatcccaccc tgcgctgctg cgcgccctgg tcgccgctct cgccctcagc
     1201 ctggccgcgc cagccccgct gtgggcgcaa aagggccgcg agccggtgac gctgaatttc
     1261 gtcaatgccg acatcgaggg cgtggcgcgc gcgatggggg ccatcctcga tcggcaaatc
     1321 gtggtcgacc cgcgcgtcaa gggcaacatc acgctctaca gcgagcagcc catgccgccg
     1381 agccaggcct tcctcagctt ccagaccgcg ctgcgcggcc tgggcttcgc ggtggtcgag
     1441 gtcgagggca tcctgaaggt ggtgcccgag gccgacgcca agctgcagac cggcaccgtc
     1501 tcggtgggcg cggtgaaccg caagggcgac cagatcatca cgcagatctt ccgcctgcag
     1561 cacgagaacg ccaacaacct ggtggccgtg ctgaggccgc tgatcagccc caacaacacg
     1621 ataaacgtca acccgggcaa caacagcctg gtcatcaccg actacgccga caacctgcag
     1681 cgcatcgcca agatcattgc cgcgaccgac atcccgtcgg cgaccgacct ggaggtgatc
     1741 ccgctcaagc acgccatcgc ctccgacatg gccaccctgg tgcagcgcct gtccgatggc
     1801 ggcggcgccg gcgcgcaggc ggtgcagggc gccgcgggcg ggacgctcac ggtgatggcc
     1861 gatgcgcgta gcaactcgct gctggtgcgc gcgcccaacg cggcccggct ggccatggtg
     1921 aagaccgtgg tcgagaagct cgaccagccg ggccagagcg gcgccggcgg cagcaacatc
     1981 tacgtcgtct atctcaagaa tgccgacgcg acccggctcg cgcaggtgct gcgcgccgcc
     2041 tacagcgcca ctggcagcgg cggcagcgcg gccggcggtg cgtcggcgct aggcggtgcg
     2101 cagggcgcca actacccagc gaccaa
//
LOCUS       DASUCY010000462         4461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_267384, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000462 DASUCY010000000
VERSION     DASUCY010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267384"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1118
                     /locus_tag="VFV25_09210"
     CDS             <1..1118
                     /locus_tag="VFV25_09210"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEY1393546.1"
                     /translation="KRQRPMVLSGLDPTHIKAFFPTLAKVTQRLARRWRQAALAGQTI
                     DLQADLMRYTVDVTAGLAFGTDINTIESSEEVIQTHLDKILPALSGRLFAPIQYWRYF
                     KLPADYALGRHLEALSVAVEGFIAQARRRLQEHPELREHPTNLIEAMLAARDRERGAI
                     TDEDVSGNVLPMLLAGEDTTANTLAWMIWLLFKHPQAARRAAEEARAVLGGDGHPTQH
                     EQLGRLDFIEACAHETMRIKPVAPINVQQAVRDTVVGGIALPAGSLVMCLMRPAATDE
                     RHFPDAQAFCPERWLTGQSASSSKRVAMPFGAGPRICPGRYLALAEIKMVAAMLLASF
                     SIEAIETPDGADAQERLAFTMSPVGLKMRLRSAGGEA"
     gene            complement(1147..1851)
                     /locus_tag="VFV25_09215"
     CDS             complement(1147..1851)
                     /locus_tag="VFV25_09215"
                     /inference="COORDINATES: protein motif:HMM:NF016974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HEY1393547.1"
                     /translation="MDYHGAKSAVNRFLLHYAGWDQRPIFFDIDAICPELHELERAYP
                     KIRAELDALLEQRIDMPRYHVVNPPAKEISASTEGAWKVFMLELLGQRPERNRAHCPA
                     TCAALEKVPGILQAFFSVLEPGKSVPLHDGPYIGYLRYHLGVRVPKDDPPLIRVAGRE
                     YVWKEGEGVLFDDSWPHEVINHSHEPRVVLIVDLPRPLPLVPRLVNNFVLWGVAAPSY
                     AKKVIGKVDGYGGDLM"
     gene            complement(1998..>4461)
                     /locus_tag="VFV25_09220"
     CDS             complement(1998..>4461)
                     /locus_tag="VFV25_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HEY1393548.1"
                     /translation="ACSPRVEGVHLRGGKVARGGLRWSDRMEDYRTEVLGLVKAQQVK
                     NAVIVPVGSKGGFVLKRPPPAGDREALMKEGVACYQAYLRGLLDLTDNLVAGKVVPPP
                     YAVRHDGDDPYLVVAADKGTATFSDIANGIAAEYGFWLGDAFASGGSAGYDHKKMGIT
                     ARGAWESVKRHFRALGHDTQTQDFTVVGVGDMSGDVFGNGMLLSRHIRLLAAFDHRHI
                     FIDPDPDAATSFAERERMFALPRSSWADYDAKLISAGGGVWPRSAKAIPLPPEARAAL
                     GMEAGDGEPLTPNEVMHAILQAPADLFYNGGIGTYVKAAGESHAECGDRANDAIRVNG
                     AELRCKVVAEGGNLGCTQRGRIEYALRGGLVNTDAIDNSAGVDCSDHEVNIKILLNAV
                     VAEGDLTAKQRDLLLAEMTDDVAALVLRDNTFQNQVLAVTCAAGADLLEEQARYMRHL
                     AGAGRLNRRLEFLPSDEVLTERQQAHMGLTPPELAVLLAYHKMELYDQLLASDVPEDP
                     TLATTLQRYFPPRLRERFAQQLQRHPLRREIIATHVVNSMVNRVGPTFVHRLHEETGA
                     APPDIVRAYMVTREAFDLVSLWLANEALDHQVGVQTQNEIVLASVRQIERGTVWLLHQ
                     REALRDLDATIRRFAPGVAEVGAGLEGWLAPHERAALDAEAARFAALGVPQALAQRVA
                     RLEAQVSALDIVEVGAEVGAPVETVAGVYFGVGGGLDLGWLSQQIGRLPAGSRWQGLA
                     RVAMRNDLTSLARALAHSVLRVATAASPAAAEPAALIAAWETQREFQLARCRQLLADL
                     KPAPTLDMAMLSVLLRELRALV"
BASE COUNT          681 a         1551 c         1536 g          693 t
ORIGIN      
        1 ggaagcgcca gcggcccatg gtgctcagcg ggctggaccc gacccacatc aaggctttct
       61 tccccacgct ggccaaggtc acgcagcgcc tcgcgcggcg ctggcggcag gccgcgctgg
      121 ccggccagac gatcgacctg caggccgacc tgatgcgcta caccgtcgat gtcacggcgg
      181 ggctagcctt cggcaccgac atcaacacga tcgaatccag cgaggaggtc atccagaccc
      241 atctcgacaa gatcctgccc gcgctgtccg ggcgcttgtt cgcgcctatc cagtactggc
      301 ggtatttcaa gctgccggcc gactacgcgc tgggccggca cctggaggca ctgagcgtcg
      361 cggtggaggg cttcatcgcg caggcacgcc ggcggctgca ggagcatccc gagctgcgcg
      421 agcacccgac gaacctcatc gaggccatgc tggccgcgcg cgaccgcgag cgcggggcga
      481 ttaccgacga ggacgtgtcc ggcaatgtcc tccccatgct gctcgcaggc gaagacacga
      541 ccgccaatac cttggcctgg atgatctggc tgctcttcaa gcacccgcag gcggcacggc
      601 gggctgcgga agaggcgcgc gccgtgctcg gcggcgacgg ccacccgacg cagcacgagc
      661 agctcggccg gctcgacttc atcgaagcct gcgcccacga gacgatgcgc atcaagccgg
      721 tcgcgcccat caacgtgcag caggcggtgc gcgacaccgt ggttggcggc attgcactgc
      781 cggcgggctc gctggtgatg tgtctgatgc gcccggcggc gacggatgag cgccatttcc
      841 cggacgccca ggccttttgt cccgagcgct ggctgacggg gcagtcggcc agctcttcca
      901 agcgcgtggc gatgcccttc ggcgccgggc cgcgcatctg cccggggcgc tacctggcgc
      961 tggccgagat caagatggtg gcggcgatgc tgctggcctc cttttccatc gaggcgatcg
     1021 agacgcccga tggcgccgac gcgcaggagc gcctggcgtt tacgatgtcg ccggtgggcc
     1081 tgaagatgcg gctgcgatcc gcgggcggcg aggcctgact cggctccata cgaaccgacg
     1141 gcgcggctac atcagatcgc cgccgtatcc gtccaccttg ccgatgacct tcttcgcgta
     1201 agacggagcc gccacgcccc acagcacgaa gttgttgacc agccggggca ccaggggcag
     1261 ggggcgcggc aggtcgacga tgagcaccac gcgcggctcg tggctgtggt tgatcacctc
     1321 gtgcggccag gagtcgtcga acagcacgcc ttcaccttcc ttccagacat attcccgtcc
     1381 ggcgactcga atcagcggcg ggtcgtcctt cggcacgcgc acgcccaggt gatagcgcag
     1441 gtaaccgatg taaggaccat cgtgcagcgg caccgatttg ccgggttcca gcaccgagaa
     1501 gaaggcctgc aatatgccgg gtaccttttc gagggcggcg caggtggcag ggcagtgcgc
     1561 acggttgcgc tccggcctct ggccgagcaa ctccagcatg aacaccttcc aggcgccctc
     1621 ggtggaggcc gaaatctcct tggctggcgg gttgaccacg tgataccggg gcatgtcgat
     1681 gcgctgctcc agcaaggcgt ccagctcggc gcggatcttc ggatacgccc gctcgagctc
     1741 gtgcagctcg gggcagatgg cgtcgatatc gaagaagatg ggacgctggt cccatcctgc
     1801 atagtggagc aggaatcggt taactgcgct cttggctccg tgatagtcca tgggcaatcc
     1861 ttcctcccga catacgtcga tcgaggatca cgaggacagc ctatcaatcg ccggcgagcc
     1921 ccacaggcat gagggttttg acgtgactcg gcgctggccc aggccgcctg cgaggcgatc
     1981 ccgggccctc ctgcgactca caccagcgcg cgcaactccc tcagcagcac cgagagcatg
     2041 gccatgtcca gcgtcggcgc cggcttcagg tcggccagca gctgccggca gcgcgcgagc
     2101 tggaattcgc gctgggtttc ccaggccgcg atcagcgcgg cgggttcggc ggctgcgggg
     2161 ctcgcggccg tcgcgacgcg cagcaccgaa tgcgccagcg cacgcgccag cgaagtgagg
     2221 tcgttgcgca tcgccacgcg cgccaggcct tgccagcggc tgccggccgg caggcggccg
     2281 atctgctgcg acagccagcc caggtcgagc cccccgccga cgccgaagta gacgcccgcg
     2341 accgtttcca ccggcgcacc gacttctgca ccgacctcga cgatgtcgag cgccgagacc
     2401 tgggcctcca gccgcgcgac gcgctgcgcc agcgcctgcg gcacgcccag cgctgcgaag
     2461 cgggccgcct cggcatcgag cgcggcgcgc tcgtgcgggg ccagccagcc ttccagtccc
     2521 gcgccgactt cggcgacgcc gggcgcgaag cggcggatgg tcgcatccag gtcgcgcagc
     2581 gcctcgcgct ggtgcagcag ccagaccgtg ccgcgctcga tctgccgcac gctggccagc
     2641 acaatctcgt tttgcgtctg cacgccgacc tggtgatcga gtgcttcgtt ggccagccac
     2701 agcgagacga ggtcgaaggc ctcgcgcgtg accatgtagg cgcgcacgat atccggcggg
     2761 gcggcgccgg tctcctcgtg caggcggtgc acgaaggtcg gccccacgcg gttgaccatg
     2821 ctgttcacca catgggtcgc gatgatctcg cgccgcagcg gatggcgctg cagctgctgc
     2881 gcgaagcgct cgcgcaggcg tggtgggaaa tagcgctgca gcgtggttgc cagcgtcggg
     2941 tcctcaggca cgtcggaggc gagcagctgg tcgtacagct ccatcttgtg ataggccagc
     3001 agcaccgcga gctccggcgg cgtgaggccc atgtgggcct gctggcgctc ggtcagcact
     3061 tcgtcggacg gcaggaactc gagcctgcga ttcagccgtc ccgcaccggc cagatgacgc
     3121 atgtagcggg cctgctcttc gagcagatcg gcgccggcgg cgcacgtgac ggccagcacc
     3181 tggttctgga aggtgttgtc gcgcagcacg agcgccgcca cgtcatcggt catctcggcc
     3241 agcagcaggt cgcgctgctt ggccgtcagg tcgccttcgg ccaccacggc attgagtagg
     3301 atcttgatgt tgacctcgtg atccgagcag tccacgccgg ccgagttgtc gatcgcatcg
     3361 gtattgacca gcccgccgcg caatgcgtat tcgatgcgcc cgcgctgcgt gcagccgagg
     3421 ttgccgcctt cggccaccac cttgcagcgc agctccgcgc cgttgacgcg gatcgcgtcg
     3481 ttggcgcggt cgccgcactc ggcatggctc tcgccggccg ctttgacata ggtgccgatg
     3541 ccgccgttgt agaacaggtc ggccggcgcc tgcaggatgg cgtgcatcac ttcgttgggc
     3601 gtgagcggtt cgccgtcgcc ggcttccatg cccagcgccg cccgcgcctc gggcggcagc
     3661 gggatggcct tcgcgctgcg cggccacacg ccgccgccgg ccgagatcag cttggcgtcg
     3721 tagtcggccc aggacgagcg cggcagggcg aacatgcgct cgcgctcggc aaagctggtc
     3781 gccgcatcgg gatcggggtc gatgaagata tggcgatggt cgaaggcggc cagcagccgg
     3841 atgtggcgcg acaacagcat gccgttgccg aacacgtcgc cgctcatgtc gccgacgccc
     3901 accacggtga aatcctgggt ctgcgtgtcg tggcccaggg cgcggaagtg gcgcttgacc
     3961 gactcccagg cgccgcgcgc ggtgatgccc atcttcttgt ggtcgtagcc ggccgatccg
     4021 ccggaggcga aggcgtcgcc gagccagaag ccgtattcgg ccgcgatgcc gttggcgatg
     4081 tcggagaagg tcgccgtgcc cttgtcggcc gcaacgacga gataggggtc gtcgccgtcg
     4141 tggcgcaccg cgtagggcgg cggcacgacc ttgcccgcga ccaggttgtc ggtaaggtcc
     4201 agcagcccac gcagataagc ctggtagcag gccacgccct ccttcatcag cgcctcgcgg
     4261 tcgccggccg gcggcggccg cttgagcacg aagccgccct tgctgcctac cggcacgatc
     4321 acggcgttct tcacctgctg cgccttcacc aggcccagca cctcggtgcg gtagtcctcc
     4381 atgcggtcgg accagcgcag cccgccgcgc gcgaccttgc cgccgcgcag gtggacgcct
     4441 tcgacccgcg gcgagcaggc g
//
LOCUS       DASUCY010000463         3531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_829736, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000463 DASUCY010000000
VERSION     DASUCY010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829736"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..172)
                     /locus_tag="VFV25_09225"
     CDS             complement(<1..172)
                     /locus_tag="VFV25_09225"
                     /inference="COORDINATES: protein motif:HMM:NF022446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HEY1393549.1"
                     /translation="MQSSAAIWLVLLLMALAANLPFVNERLFVLGPRRSPKALGWRML
                     ELLVFGAVATAIG"
     gene            complement(174..1655)
                     /gene="nuoN"
                     /locus_tag="VFV25_09230"
     CDS             complement(174..1655)
                     /gene="nuoN"
                     /locus_tag="VFV25_09230"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEY1393550.1"
                     /translation="MNDMNWLAVYPEILLLAMSCVIALVDLFVKSPGRTLTYVLSLFT
                     LAVVAGMHLAYYEGGFTLYAMQHMMVADPMGHLLAFFATVAVMMTLVYARPYAADRDI
                     LKGELFTLSLFSLLGISVMVSGNNFLVIYLGLELMSLSLYALVALRRDHVASTEAAMK
                     YFVLGALASGFLLYGLSMMYGATGSLDLGEVFKVISSGEPNMQVLVFGVVFIVAGLAF
                     KLGAVPFHMWIPDVYQGAPTAVTLLVAGAPKLAAFAIAIRLLVEGMIGLAVDWQQMLI
                     VLAVASLVIGNFVAIAQSNLKRMLAYSTIGQMGFMLLGLTAGVVNGNTLSAANAYSSA
                     MFYMVVYVLTTLGTFGLIMVLARQGFESEEIDDLKGLNQRNPVLAAVMAVFMFSLAGV
                     PPAVGFYAKLAVLQALVSTNVTGYIVLAVAAVVLSLIGAYYYLRVVKVMYFDEPSDPS
                     KLTASGDAYALLSLNGAAVLVLGVLPNGLMAMCANAIVIALTT"
     gene            complement(1672..3147)
                     /locus_tag="VFV25_09235"
     CDS             complement(1672..3147)
                     /locus_tag="VFV25_09235"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEY1393551.1"
                     /translation="MPYLSLAIWLPVVIGMALLAMGRSEQANAVRWIALVGSVASFLV
                     TIPLITGFDTGTAAMQFSENLSWIERFNVHYHLGVDGISVWFVLLTAFITIIVVMSAW
                     EVITERVNQYMGAFLILSGLMVGVFSALDGLLFYVFFEATLIPMYIIIGMWGGPRRVY
                     AAFKFFLYTLLGSLLFLVALIYLYYKSGGSFEILAWHQLPLSAQAQTLLFFAFLAAFA
                     VKVPMWPVHTWLPDAHVEAPTGGSVVLAAIMLKLGAYGFLRFSLPILPDASHEWAGFI
                     IAISLIAVIYIGLVALVQNDMKKLVAYSSVAHMGFVTLGFFIFNELGISGGIVQMISH
                     GFVSGAMFLCIGVLYDRVHSREIAAYGGVVNTMPKFAAFAVLFAMANCGLPGTAGFVG
                     EWMVILGTVRTNFWLAFLAATALISGAAYTLWMVKRVYFGDVANKDVGALDDINKREF
                     AMLALLAAAVLWMGVYPKPFTDVMHASVSELIRHASISKLN"
     gene            complement(3162..>3531)
                     /locus_tag="VFV25_09240"
     CDS             complement(3162..>3531)
                     /locus_tag="VFV25_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEY1393552.1"
                     /translation="LPFWLALAGVVSAYVFYLVKPAIPAAIKARFGFIYTILENKYYM
                     DWINENIIAVAARLVGRGLWKGGDAGLIDGLAINGSARLVAWSASVMRLFQSGYIYHY
                     AFVMIVGVFALMTWFVWLHK"
BASE COUNT          771 a         1127 c         1135 g          498 t
ORIGIN      
        1 tgccgatggc agtcgcgacg gcgccgaaga cgaggagttc gagcatccgc caacccaggg
       61 ccttgggcga gcggcgcggc cccagcacga acagccgctc gttgacgaag ggcagattgg
      121 ccgccagagc catcagcagg agcacgagcc agatggcggc cgaggactgc atctcaggtc
      181 gtcagagcga tcacgatggc attggcgcac atcgccatca aaccgttggg cagcacgccg
      241 agcaccagca ccgctgcgcc gttgagcgac agcagggcat aggcgtcgcc gctcgccgtg
      301 agcttgctcg gatcgctggg ctcgtcgaaa tacatgacct tgacgacgcg cagatagtag
      361 taggcgccga tcagcgacag caccacggcc gccacggcca gcacgatgta gccggtgacg
      421 ttggtggaca cgagggcctg cagcaccgcg agcttggcat agaagcccac cgcgggcggc
      481 acgccggcaa gcgagaacat gaacacggcc atcacggcag ccagcacagg attgcgctgg
      541 ttcagcccct tgaggtcgtc gatctcttcg gactcgaagc cctggcgcgc cagcaccatg
      601 atgaggccga aggtgcccag cgtggtcagt acatagacca ccatgtagaa catcgccgag
      661 ctgtaggcat tggcggccga caaggtgttg ccgttgacca cgccggccgt caggcccagc
      721 agcatgaagc ccatctggcc gatcgtcgaa taggccagca tgcgcttgag attgctctgc
      781 gcgatggcaa cgaagttgcc gatcacgagc gaggccacgg ccagcacgat cagcatctgc
      841 tgccagtcca cggccaggcc gatcatgcct tccaccaaca ggcggatcgc gatcgcgaag
      901 gcagccagct tgggcgcccc ggcgacgagc agggtcacgg ccgtcggcgc tccctgatag
      961 acatcgggga tccacatgtg gaaaggcacg gcacccagct tgaaggccag gccggccacg
     1021 ataaagacga cgccgaacac cagcacctgc atgttgggct cgccgctgct gatgaccttg
     1081 aagacctcgc ccaggtccag cgagccggtg gccccgtaca tcatcgacag gccatagagc
     1141 aggaagccgc tggccagggc gccgagcacg aaatacttca tcgccgcttc ggtcgaggcg
     1201 acgtggtcac ggcgcagcgc caccagcgca tagagcgaca gtgacatcag ctccaggccc
     1261 aggtagatga ccaggaagtt gttgcccgac accatgacgg agatgcccag cagcgagaac
     1321 aggctgagcg tgaacagctc gcccttcagg atgtcgcgat cggcggcata gggtcgcgca
     1381 tagaccagtg tcatcatcac ggccaccgtc gcgaagaagg ccagcagatg gcccatcggg
     1441 tcggccacca tcatgtgctg catcgcatag agcgtgaagc cgccctcgta gtaggccagg
     1501 tgcatgccgg cgaccaccgc cagcgtaaac aggctcagca cataggtgag cgtgcggccc
     1561 gggctcttga cgaagaggtc gaccaaggcg atcacgcagc tcatcgccag caagaggatc
     1621 tcgggataga ccgcgagcca gttcatgtca ttcatcgcgt cgggctccgg ctcaattcag
     1681 cttggagatg ctggcatgcc tgatcagctc gctgaccgag gcatgcatca cgtcggtgaa
     1741 gggcttgggg tagacgccca tccacagcac cgccgccgcc agcagcgcca gcatggcgaa
     1801 ttcgcgcttg ttgatgtcgt ccagtgcgcc gacgtccttg ttggccacgt cgccgaaata
     1861 gacgcgcttg accatccaca gcgtgtacgc ggcgcccgag atcagcgccg tggcggccag
     1921 gaaggccagc cagaagttcg tgcgcacggt acccaggatc accatccact cgccgacgaa
     1981 gccggccgta cccggtaggc cgcagttggc catcgcgaac agcaccgcga aggctgcgaa
     2041 cttcggcatg gtgttgacga caccgccata ggccgcgatc tcgcgcgaat gcacgcggtc
     2101 gtagagcacg ccgatgcaca ggaacatcgc gcccgagacg aagccgtgcg agatcatctg
     2161 cacgatgccg ccggagatgc ccagctcgtt gaagatgaag aagcccagcg tcacgaagcc
     2221 catgtgggcc accgacgaat aggccaccag cttcttcatg tcgttctgca ccagcgcgac
     2281 caggccgatg tagatcaccg cgatcagcga gatcgcgatg atgaagccgg cccattcatg
     2341 gctggcgtcc ggcaggatcg gcagcgagaa gcgcaggaag ccgtaggcgc ccagcttcag
     2401 catgatggcc gccagcacca ccgagccgcc ggtgggcgcc tcgacgtggg cgtccggcag
     2461 ccaggtgtgc accggccaca tcggcacctt gaccgcgaag gcagccagga aggcgaagaa
     2521 gagcagcgtc tgggcctgcg ccgacagcgg cagctggtgc caggccagga tctcgaagct
     2581 gccgcccgac ttgtagtaca ggtagatcag cgccaccagg aagagcagcg agccgagcag
     2641 cgtgtagagg aagaacttga aggccgcata gacgcgccgc ggcccgcccc acatgccgat
     2701 gatgatgtac atcgggatca gcgtggcctc gaagaagacg tagaacagca ggccgtccag
     2761 cgccgagaac acgccgacca tcaggcctga caggatcagg aaggcgccca tgtactggtt
     2821 gacgcgctcg gtaatcactt cccaggccga catcacgacg atgatcgtga tgaaggcggt
     2881 aagcagcacg aaccacaccg agatgccgtc cacaccgagg tggtaatgga cgttgaagcg
     2941 ctcgatccag ctcaggtttt cgctgaactg catcgcggcg gtaccggtgt cgaagccggt
     3001 gatgagcgga atggtgacga ggaagctcgc gacggagccg accagggcga tccagcgcac
     3061 cgcgtttgcc tgctcgctgc ggcccatggc cagcagcgcc atgccgatga cgaccggcag
     3121 ccagatggcg agggagaggt aaggcatgtc tcagtccccg ctcacttgtg cagccacacg
     3181 aaccaggtca tcagcgcgaa gacgccgacg atcatgacga aggcatagtg gtagatgtag
     3241 ccggactgga aaagccgcat caccgacgcc gaccacgcga cgagacgggc cgagccgttg
     3301 atggccaggc cgtcgatcag gccggcgtcg ccacccttcc acaggccgcg acccaccagg
     3361 cgagccgcga cggcgatgat gttctcgttg atccagtcca tgtagtactt gttttccaag
     3421 atcgtgtaga tgaagccgaa gcgcgccttg atggccgccg gaatcgccgg cttcaccagg
     3481 tagaacacat aggccgagac cacgccggcc agcgccagcc agaacggcag c
//
LOCUS       DASUCY010000464         3007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_379997, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000464 DASUCY010000000
VERSION     DASUCY010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379997"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..904
                     /locus_tag="VFV25_09245"
     CDS             122..904
                     /locus_tag="VFV25_09245"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEY1393553.1"
                     /translation="MLEVSIATRSECGMRDHNEDALRTGREDSVCYAVLADGAGGHQG
                     GEEAARRVVTNMERGLRDAASRFGPEALTQLVLAAHDELQRVQTQAQGVDRMHATVVA
                     IWMDTRTDHALWTHVGDSRLYRLRHGVVDYVTTDDSVVQRMVEAGLLTPDQARHHPQK
                     SQLIAALGIVDAVEPHTTEYAVELEDGDAYLLCSDGWWEPLDAHSIACMLEEAGSPEE
                     WLDLMQRRIEERALPRQDNFSAIGLWVGDPAEATRIMPEEFP"
     gene            complement(901..1590)
                     /gene="tagF"
                     /locus_tag="VFV25_09250"
     CDS             complement(901..1590)
                     /gene="tagF"
                     /locus_tag="VFV25_09250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagF"
                     /protein_id="HEY1393554.1"
                     /translation="MNAGDLHELPGWYGKLSPLGDFAQRRLPADWVQACDAWLSAVMR
                     ESRQRLGERWLDVYLTAPLQRFAWAPGVVDSAWWFGVLMPSCDKVGRYFPLLIAQPRS
                     QPPTDRIALDHLDLWYEHLARAAMQTLHEAASLEAFETELEAAPPWPVPARLLAVQAQ
                     PLGLGERYAFAAAAPLSQWLPALAAQDLLNRLAGCTLWWRMGEADSAAGATLMHGLPD
                     AGGFIDLLSSG"
     gene            complement(1620..>3007)
                     /gene="tssM"
                     /locus_tag="VFV25_09255"
     CDS             complement(1620..>3007)
                     /gene="tssM"
                     /locus_tag="VFV25_09255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HEY1393555.1"
                     /translation="KQLDEEQGWVLGIADAPRNVATSLRGGQPLVDDVKRLYLTDYAS
                     TWESFVADLRLQPMTSSLTQSVQMARLLSASNSPLPPLLKAISRETTLLAGDKGLLGK
                     AGEAATGIFKKSSQALTDKLPLGTAGAADAGPPLESIVDDRFAGLRQMVTAPEGGKAP
                     LDDTIALIGEVYVLLNAVDTAVKSKSAPPASPVPNKVKAEAARLPEPLRPMLETLAQS
                     SGQVAQTVVRQNLSGEVRSQVGEFCAKAIAGRYPINRGSSIDITQADFAQLFAPGGKM
                     DQFFQQQLAPHVDTSTRPWSFRQEEGGALGKDYGSLPQFQRAQAIRETFFAAGNTPSL
                     RLSFKPIEMDASIQHFILDVDGQKVLYEQGPPIPTTVQWPGPRGSSQVRVDISPTRPG
                     ATSGMVNDGPWALFHLLDRVKLEPAGAPERFRATFNIDGRKAVFEVTASSVRNPFLMP
                     ELREFSCPMGL"
BASE COUNT          469 a          993 c         1074 g          471 t
ORIGIN      
        1 atacgcagcg aagcccagga cgacttccag accctcttcg gcaaggcctt cctggcggcc
       61 tacgagcagc agctcgaacg gctgcggcgc gagaaatccg acacctgacc ctaagtccat
      121 catgctcgag gtctcgatcg ccacgcgcag cgaatgcggc atgcgcgacc ataacgagga
      181 cgcgctgcgc acgggccgcg aagattccgt ctgctatgca gtgctggccg atggtgcggg
      241 cggccatcag ggcggcgagg aggcggcgcg ccgcgtggtc accaatatgg aaagagggct
      301 gcgtgatgcg gcgtcgagat tcggccccga ggcactgacg cagctggtgc tggcggctca
      361 tgacgagctg cagcgcgtgc agacccaggc gcagggcgtc gatcgcatgc acgccaccgt
      421 ggtcgcgatc tggatggaca caaggaccga ccatgcgctg tggacccatg tcggcgactc
      481 gcgcctgtac cggctgcgcc atggcgttgt cgactatgtc accaccgacg acagcgtggt
      541 gcagcgcatg gtcgaggccg gtctcctgac gcccgaccag gcccggcatc atcctcagaa
      601 gagccagctg atcgccgccc tgggcattgt cgacgcggtc gagccgcaca ccaccgagta
      661 cgcggtcgag ctggaagacg gcgatgccta tctgctctgc agcgacggct ggtgggagcc
      721 gctggacgcg cacagcatcg cctgcatgct ggaggaggcg ggcagccccg aggaatggct
      781 ggacctgatg caaaggcgca tcgaggagcg ggcgctgccg cgccaggaca acttcagtgc
      841 catcggcttg tgggtcggtg atccggccga ggcgacgcgc atcatgccgg aagagtttcc
      901 ctagccgctg ctcagcaggt cgatgaagcc gccggcatcc ggcaggccat gcatcagcgt
      961 ggccccggcg gcgctgtccg cttcgcccat gcgccaccag agcgtgcagc cggccagccg
     1021 gttcagcagg tcctgtgcgg cgagcgcggg cagccattgc gagagcggcg ctgctgccgc
     1081 gaaggcgtag cgctcgccca gacccagcgg ctgggcctgc acggcgagca gccgagccgg
     1141 cacgggccaa ggcggggccg cttccagctc ggtttcgaag gcctcgagcg aggccgcctc
     1201 gtgcagcgtc tgcatcgcgg cgcgggctag gtgctcgtac cagagatcca ggtgatccag
     1261 tgcgatgcga tcggtgggag gctggctgcg cggctgggcg atcagcagcg gaaagtagcg
     1321 cccgaccttg tcgcagctgg gcatcaggac gccgaaccac cacgcagagt cgaccacgcc
     1381 cggcgcccag gcgaagcgct gcagcggcgc ggtgaggtag acatcgagcc agcgctcgcc
     1441 aagccgttgt cggctctcgc gcatcacggc cgatagccag gcgtcgcaag cctgcaccca
     1501 atcggccggc aggcggcgct gcgcgaagtc acccaagggg gagagcttgc cgtaccagcc
     1561 cggcagctcg tgcagatcgc cggcgttcat ggctgggctc ggaccaggca gcgggcgcgc
     1621 tacaagccca tcgggcagga gaactcgcgc agctcgggca tcaggaaggg gttgcgcacg
     1681 ctgctggctg tcacctcgaa cacggccttg cggccatcga tgttgaaggt ggcgcggaag
     1741 cgctcgggcg ctccggcggg ttccagtttc acgcggtcca gcaagtggaa cagcgcccag
     1801 gggccgtcgt tcaccatgcc cgaggtggcg cctgggcggg tcgggctgat gtccacgcgc
     1861 acctgcgagc tgccgcgcgg gcccggccac tgcaccgtgg tcgggatcgg cgggccttgc
     1921 tcatagagca ccttctggcc gtccacatcg aggatgaagt gctggatcga ggcgtccatc
     1981 tcgatcggct tgaagctcag gcgcagcgag ggcgtgttgc ccgcggcgaa gaaggtctcg
     2041 cgtatcgcct gggcgcgctg gaattgcggc aggctgccgt aatccttgcc cagcgcgccg
     2101 ccttcctcct ggcggaagct ccagggccgc gtgctggtgt ccacgtgggg tgcgagctgc
     2161 tgctggaaga actggtccat cttgccgccc ggcgcgaaga gctgcgcgaa gtcggcctgg
     2221 gtgatgtcga tcgacgagcc gcggttgatc ggataacgcc ctgcgatggc ctttgcgcag
     2281 aactcgccga cctgcgatcg gacctccccg gagaggttct gtcgcaccac ggtttgcgct
     2341 acctggccgc tgctttgcgc cagcgtctcc agcatgggcc tcaggggctc aggcagccgc
     2401 gccgcctcgg ccttgacctt gttgggcacc ggcgacgcgg gcggagccga cttgctcttg
     2461 accgccgtgt ccacggcgtt gagcagcaca taaacctcac cgatcagcgc gatcgtgtcg
     2521 tccagcggtg ccttgccccc ctcgggggcg gtgaccatct ggcgcagccc cgcgaagcgg
     2581 tcgtcgacga tgctttccag cggcgggcct gcgtcggcgg caccggctgt gccgagcggc
     2641 agcttgtcgg tcagcgcctg gctggacttc ttgaagatgc cggtggcggc ttcgccggcc
     2701 ttgccgagca ggcccttgtc gccggccagc agcgtggtct cgcgcgagat cgctttcagc
     2761 aacggtggca gcgggctgtt ggaggccgac agcaggcgcg ccatctgcac cgattgcgtg
     2821 aggctgctgg tcatcggctg caggcgcagg tcggcgacga agctttccca ggtgctggcg
     2881 tagtcggtta ggtagaggcg cttcacgtcg tcgaccagcg gctggccgcc gcgcagactg
     2941 gtggcgacat tcctgggcgc gtcggctatg cccagcaccc agccctgctc ctcgtcgagc
     3001 tgcttcg
//
LOCUS       DASUCY010000465         4275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_621021, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000465 DASUCY010000000
VERSION     DASUCY010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4275
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621021"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1034
                     /locus_tag="VFV25_09260"
     CDS             <1..1034
                     /locus_tag="VFV25_09260"
                     /inference="COORDINATES: protein motif:HMM:NF017770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HEY1393556.1"
                     /translation="VRKLRYFYGNTRIAGHAFDFHGNPRRMLRGYLLVGALIVLYSVA
                     GKVSPTAGLVALVIVAVIWPALLRASMQFRLANTSWRGLRFRFTGSTAQAYRAVLPLF
                     IPALLFLGIVPLVGPQPGSTPATASEAARTMGGVMLAAGLIAALVGPWAWWLLKRYQH
                     DHYALGDEQTRLRAGVGPFYALSLKTGGIALLMGLVLGVLGAIGGAAMFATARGLDSK
                     AMGIVAIAALVAGGYILLIAVVGSYSTSRTQNLVWTQTASPHLRFKSDLRFGALLRLT
                     LMNWLLVALTLGFYWPFAKVAMTRLRLEAIRLGSSMPIDTLANSVRQRADDASGDAAG
                     ELFGIDIGL"
     gene            1031..2092
                     /locus_tag="VFV25_09265"
     CDS             1031..2092
                     /locus_tag="VFV25_09265"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEY1393557.1"
                     /translation="MNDAHQAFADADYFDGLTAAPRRVRLRIAGPRLLIEGDGISLAV
                     AVSQVQWPERQRHGARIAHLAGGGSLQACDAASWDAWLRAAGRSDSLAVRLQQSWRGS
                     LLAAALLLALVLAGYRWGLPLAARGVLAWVPASVDRQVGDAALASIDGRWLMASTLPP
                     EQRQRIAAEFERAVALAYPPGGRPAYELQFRKSKLGPNAFALPGGTIVLTDEMVRLLD
                     QREEVLMGVLGHELGHVRRRHSMRLFVQATLLGAVSSAAFGDFSGLLAGAPALLGHLD
                     YSRDFEREADDEAIAVLRANGQSPALMVELFEALERQRKAKADKDGGEGLAQRLGIAL
                     STHPADAERMQRFRDAARR"
     gene            2125..3171
                     /gene="pyrC"
                     /locus_tag="VFV25_09270"
     CDS             2125..3171
                     /gene="pyrC"
                     /locus_tag="VFV25_09270"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEY1393558.1"
                     /translation="MSSSTPPASLTITRPDDWHLHLRDGTALQAVVPDSARQFARAIV
                     MPNLRPPVTTTAQALAYRERILAAVPQGLAFEPLMTLYLTDNTPPEEIQQAREAGVVA
                     LKLYPAGATTNSDAGVTDIRKTYKTLEAMQRAGLKLLVHGEVTDPAVDVFDREAVFIE
                     RHLIPLRRDFPELKIVVEHITTKEAAQYVSEADAHTAATITAHHLLYNRNAIFTGGLR
                     PHFYCLPVLKREEHRAALVAAATSGSAKFFLGTDSAPHPAHLKEHASACAGCYTALSA
                     MELYAEAFEAAGALDKLEAFASFNGPDFYGLPRNSGTLSLRRDSWTLPESLPFGEAQL
                     KPLRGGETLAWRVG"
     gene            3205..3981
                     /locus_tag="VFV25_09275"
     CDS             3205..3981
                     /locus_tag="VFV25_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEY1393559.1"
                     /translation="MNHTDPKEATRREARIALLIDADNSPAAKIDVILAELATFGETN
                     IRRAYGNWKKNELKGWEEALHEHAIRPMQQFDYSKGKNASDMAMVIDALELLYTDRPD
                     AYGIVSSDADFTPLVMHLRAKGAAVYGFGAKKTPEPFVNACSRFLFLDQLADASSDAA
                     AAPAAPLRVPAAQLKQDARLVALLRNAVNAAVDESGWARVGVVGQQIANQASLDPRNY
                     GYATLTKLLAATQLFEMNNEGTSQVAVRDKREAKAATKGG"
     gene            3978..>4275
                     /locus_tag="VFV25_09280"
     CDS             3978..>4275
                     /locus_tag="VFV25_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3025 domain-containing protein"
                     /protein_id="HEY1393560.1"
                     /translation="MSFIAAIDWSRHWLAPYRALGARLAREVAAGAPVAQALNAAPAV
                     HDAAVPQLAAGPLRFVPQTELPDGEAYEAYIHRTACVPTRDNLHDFFNGLVWLHE"
BASE COUNT          683 a         1502 c         1428 g          662 t
ORIGIN      
        1 gggtgcgcaa gctgcgctat ttctacggca acacacgcat cgccgggcat gccttcgatt
       61 tccacggcaa cccccggcgc atgctgaggg gctatctgct ggtgggcgca ctgatcgtgc
      121 tgtactcagt ggccggcaag gtgtcgccca cggcaggcct ggtcgcactg gtgatcgtcg
      181 cggtcatctg gccggcgctg ctgcgcgcct cgatgcagtt ccgcttggcg aacacgagct
      241 ggcgcggtct gcgcttccgc ttcacgggca gcacggcgca ggcttatcgt gcggtactgc
      301 ccctgttcat ccctgcccta cttttcctcg gcatcgtgcc gctggtcggt ccgcagccgg
      361 gaagtacacc tgccaccgca tcggaggcgg caagaacgat gggaggggtg atgctggcgg
      421 ccggcctgat tgccgcgctg gtcgggccct gggcttggtg gctgctcaag aggtatcagc
      481 acgaccacta cgccttgggt gacgagcaaa ccaggctgcg ggccggcgtc ggaccgttct
      541 atgcactgtc gctcaagacg ggcgggatag ccttgctgat ggggctggtg ctgggcgtgc
      601 tcggtgctat cggaggcgcc gccatgttcg ccacggcccg cggcttggat tccaaggcca
      661 tgggcatagt ggccatcgcc gcactggtcg ccggcggcta catcctgctg attgcagtgg
      721 tcggctccta cagcacctcg cgcacccaga atctggtgtg gacgcagacg gccagcccgc
      781 acctgcgctt caagagcgat ctgcggttcg gcgcgctgct gcggctgacg ctgatgaact
      841 ggctgctcgt tgcgctgacg ctgggctttt actggccctt cgccaaggtc gccatgacac
      901 gcctgcggct cgaggccatt aggctaggca gcagcatgcc gatcgacacc ctcgccaaca
      961 gcgtccgcca acgcgccgat gatgcgagcg gcgatgccgc cggcgagctg ttcggcatcg
     1021 atatcgggct gtgaacgacg cgcatcaggc cttcgccgac gccgactact tcgacggcct
     1081 gacggccgcg ccgagacgtg tccggctgcg catcgccggc ccccgcctgc tgatcgaggg
     1141 cgacggcatc tcgctcgccg tggcggtgtc gcaagtgcaa tggcccgagc gccagcggca
     1201 tggcgcgcgc atcgcgcatc tggccggtgg cggctcgctg caggcctgcg acgcagcatc
     1261 ctgggatgcc tggctgcgtg ccgcgggccg gagcgactcg cttgccgtgc ggctgcagca
     1321 gagctggcgc ggcagcttgc tcgcggcggc cttgctgctc gcgctcgtgc tcgccggcta
     1381 ccgctggggc ctgccgctcg ccgcgcgcgg ggtgctggcc tgggttcccg ccagtgtcga
     1441 ccggcaggtc ggcgatgcgg ccctggcatc gatcgacggg cgctggttga tggccagcac
     1501 gctgccgccc gaacagcggc agcgcattgc cgccgaattc gaacgcgccg tggcgctcgc
     1561 ctatccgcct ggcgggcgcc ccgcttacga actgcagttc cgcaagagca agctaggacc
     1621 caatgccttc gccctgcccg gcggcaccat cgtgctcacc gacgaaatgg tgcgcctgct
     1681 ggaccagcgg gaggaggtgc tgatgggtgt cctgggccat gagctcgggc atgtgcggcg
     1741 gcgccacagc atgcgcctgt tcgtgcaggc aacgctgctc ggcgccgtca gcagtgccgc
     1801 cttcggcgat ttcagcggcc tgctggccgg cgcaccggcc ctgctcggcc acttggacta
     1861 ctcgcgcgat ttcgagcgcg aggccgacga cgaggccatt gcggtgctgc gtgcgaacgg
     1921 ccagtcgccg gcgttgatgg tcgagctgtt cgaggcgctg gaacgccagc gaaaggccaa
     1981 ggcagacaag gacggcggcg aaggtttggc gcaacgcctg ggcattgcgc tgtcgactca
     2041 cccggcagat gcggagcgca tgcagcgctt ccgcgatgcg gcacggcgct aggttcgctc
     2101 gcacgggtga gagaaaatcg ccccatgagc agcagtaccc ctcccgcctc cctcaccatc
     2161 acccgccccg acgactggca tctgcatctg cgcgacggca cggcgctgca agccgtggtg
     2221 ccggacagcg cacgccagtt cgcacgcgcg atcgtgatgc ccaacctcag gccgccggtc
     2281 acgacgacgg cgcaggccct ggcctatcgc gagcgcatcc tcgcggccgt cccgcaggga
     2341 ttggctttcg agcccttgat gacgctctat ctcaccgaca acacgccgcc cgaggaaatc
     2401 cagcaggcgc gcgaggccgg tgtcgtcgcg ctcaagctct acccggcggg tgccaccacc
     2461 aacagcgatg ccggcgtgac cgacatccgc aagacctaca agacgctgga ggcgatgcaa
     2521 cgcgccggcc tcaagctgct ggtgcacggc gaagtcaccg acccggcggt ggacgtgttc
     2581 gaccgcgaag ccgtcttcat cgagcgccac ctgatcccgc tgcgccgcga cttcccggag
     2641 ctcaagatcg tggtcgagca catcacgacc aaggaagccg cgcaatacgt gagcgaggcg
     2701 gacgcgcaca ccgcagccac catcacggcc catcacctgc tctacaaccg caacgccatc
     2761 ttcaccggcg gcctgcgccc gcacttctac tgcctgccag tgctcaagcg cgaggaacat
     2821 cgcgcggcgc tggtcgcggc cgccacctcg ggctcggcga agttcttttt gggcaccgac
     2881 agcgcaccgc atccagccca cctgaaagag catgccagcg cctgcgccgg ctgctacacc
     2941 gccttgagtg cgatggagct ctacgccgag gccttcgagg cagccggcgc gctcgacaag
     3001 ctggaagcct ttgccagctt caacggcccg gacttctacg gcctgccgcg caacagcggc
     3061 acgctgagcc tgcgccgcga cagctggacc ctgcccgagt cgctgccctt cggcgaggcg
     3121 cagctcaagc cgctgcgcgg cggcgagacg ctggcctggc gcgtgggctg aggctgccga
     3181 tccattcatc gcaaaggcga ggggatgaac cacaccgacc ccaaggaagc cacgcggcgc
     3241 gaggcgcgca ttgcgctgct gatcgatgcc gacaactcgc cggccgcgaa gatcgacgtg
     3301 atcctcgccg agctggccac cttcggcgaa accaacatcc gccgcgccta cggcaactgg
     3361 aagaaaaacg agctgaaagg ctgggaggaa gcgctgcacg agcacgccat ccggcccatg
     3421 cagcagttcg actacagcaa gggcaagaac gccagcgaca tggcgatggt gatcgacgcg
     3481 ctggagctgc tctacaccga ccggccggat gcctacggca tcgtctcgtc ggacgccgac
     3541 ttcacgccgc tggtgatgca tctgcgcgcc aagggcgctg cggtctatgg cttcggcgca
     3601 aagaagaccc ccgaaccctt cgtcaacgca tgctcgcggt tcctgtttct cgatcaactc
     3661 gccgatgcct ccagcgacgc cgcggccgcg cccgccgcgc cgctgcgcgt gccggccgcc
     3721 cagctgaagc aggacgcccg gctggtggcg ctgctgcgca atgccgttaa cgcggcggtc
     3781 gacgagagcg gctgggcacg tgtgggcgtg gtgggccagc agatcgccaa ccaagcctcg
     3841 ctcgatccgc gcaactatgg ctacgccacg ctgaccaagc tgctggcggc aacgcagctg
     3901 ttcgaaatga acaatgaagg caccagccaa gtcgcggtgc gcgacaagcg cgaggccaag
     3961 gccgccacca aaggcggatg agcttcattg ccgccatcga ctggtcgcgg cactggctgg
     4021 cgccgtatcg cgcgctcggc gcgcggctgg cccgcgaggt cgccgccggc gcgccggtgg
     4081 cgcaggcgct gaacgcggcg ccggcggtgc acgatgcagc cgtgccgcag cttgccgccg
     4141 gccccctgcg cttcgtgcct cagaccgaat tgccggacgg tgaagcctac gaggcctaca
     4201 tccaccgcac ggcctgcgtg cccacgcgcg acaacctgca cgacttcttc aacggcctgg
     4261 tctggctgca cgagc
//
LOCUS       DASUCY010000466         9016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1520409, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000466 DASUCY010000000
VERSION     DASUCY010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 9016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9016
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1520409"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /gene="hpnC"
                     /locus_tag="VFV25_09285"
     CDS             complement(<1..607)
                     /gene="hpnC"
                     /locus_tag="VFV25_09285"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEY1393561.1"
                     /translation="MSVDHYENFPVASLLCPPRLRPAVAALYWYARTADDIADEGDAA
                     PAQRLADLADYRAALQGAANGAQPSRWPEVFAPLGPALREFGLPLPLMEDLLSAFEQD
                     VVKTRYADREELLDYCRRSANPVGRLLLHLYGVTQAQALHESDAICSALQLINFWQDL
                     SIDLDRGRLYLPLADCARHGVEPAALLARHDSPAARALVEDV"
     gene            884..2050
                     /locus_tag="VFV25_09290"
     CDS             884..2050
                     /locus_tag="VFV25_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY1393562.1"
                     /translation="MSPSPLAAFSARPASRVAALAIVAAGALLAACSKSDPPPEPVRA
                     VRTMQIASEAAGNTLEYAAEVKARVESRLGFRVGGKLMRRAVNLGDTVKAGQLLAQLD
                     ARDLVLGEQAAKANLSAAQVSFDLAQADFKRYKDLRDQDFIGAAELDRREATLKSAKA
                     ALEQARAQANVQGNQAGYADLLADAAGVITGVDAEPGQVVAAGTPVLRLAQDGPRDVV
                     FSVPEDKVQNARALVGKAGGLKVKLWGSNEQLPAMVREIAAAADPATRTFLVKADVGR
                     NTVKLGQTATVSLDLPRTDGITKLPLTALMEQQGKTSVWLLDKASMTVKPQVVQVSGA
                     EGNAAIVTAGLSPGQLVVTGGVHVLTPGQKVKLYGVADATNDAAAAQAATEAMR"
     gene            2072..5299
                     /locus_tag="VFV25_09295"
     CDS             2072..5299
                     /locus_tag="VFV25_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY1393563.1"
                     /translation="MTQEQAQKSAAQPQRFNISRWALEHAPLTRYLMVVLMVLGIAAY
                     FQLGQDEDPPFTFRAMVVSAYWPGATAQQMAEQVTDKIERTLQEAPYADKIRSYTKPG
                     ESLTIFQLKDSSPPKEVSNAWYQVRKKIGDMRSTLPQGVLGPVFNDEFGDVYGSIYAL
                     SADGFSAEELRQFAEQVRSRLLHVPDVAKVQLFGVQDEKIFIEISQKRLAQLGIDFNQ
                     VIAQLGAQNAVEGAGVLDAAGDNVQVRVGGQFDSVEDLRAFPIRAVDPATGQASSLRL
                     GDIADIKRSYIDPPSVKVRHQGKEVVAMGISMAKGGDIIELGKHLHETVAMIQKDLPV
                     GVEMSQIQDQPRAVSRSVGEFVDVLAEAIVVVLAVSFISLGLHSSTGRLGFRIDIWPG
                     MVVAITIPLVLAITFVTMYYWGVGLHKISLGALIIALGLLVDDAIISVEMMVRKLEEG
                     YDKKRAATFAYELTAMPMLTGTLITAAGFLPIGIAKSTVGEYTFAIFAVTTAALLISW
                     LVSVYFVPYLGTLLLHTRAKVQGETHELFDTPFYNRFRALVNWCVEHRWKTIGATLAL
                     FVLGLVGMGKVQQQFFPDSSRPEILVDLWLPEGSGYVTTEAIAKRFEARMMKEPGVAT
                     VSTWVGSGVPRFYLPLDQIFPQTNVMQAILLPKDLKTREELRQRLPALLAEEFPDVRG
                     RVKLLPNGPPVPYPVQFRVIGSDVKQVRTWADEAKAVLRENPNMRGVNDNWNESVKVL
                     RLEIDQDKARALGVTSQAIAQASRTILSGTNIGQYRQGDRLLDIVLRQPLEERKAITD
                     IGNAYVPTSTGRAIPLTQIAKVGFTWEPGVLWREGRNYAVTVQGDVVEGMQGATVTAQ
                     IWPKLQQLVQRMPPGYSIEVAGAVEESSKGQGSIAVGAPLMLFIMFTLLMLQLHSVSR
                     AVLVFLTGPMGIAGVAGALLVLNRPFGFVALLGVIALMGMIMRNSVILIDQIEQDRAK
                     GVPTWNAIVESAVRRFRPIVLTAAAAVLAMIPLSRSVFWGPMAVAIMGGLIVATALTL
                     LALPAMYAAWFRVKREVPKEAPAPAAIPAEGHALMPQESPRPATS"
     gene            5358..5444
                     /locus_tag="VFV25_09300"
     tRNA            5358..5444
                     /locus_tag="VFV25_09300"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5392..5394,aa:Leu,seq:tag)
     gene            5481..6788
                     /gene="tig"
                     /locus_tag="VFV25_09305"
     CDS             5481..6788
                     /gene="tig"
                     /locus_tag="VFV25_09305"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEY1393564.1"
                     /translation="MAVTVENLEKLERRITLTLSADVLRSEVESRLKRLARTVKADGF
                     RPGKVPMSVVAQRYGYSVQYEVMNDKVGEAFSKAATEAELRVAGAPRITEKEGAPAGE
                     LAFDATFEVYPEVKLGDLSAAEIERVGTEVTEAAIDKTLDILRKQRRTFAQRPAAEGA
                     ADGDRVTIDFEGKIDGTPFEGGKADGFQFILGEGQMLEAFEKAVRGMKAGESKTFPLA
                     FPEDYHGKDVAGKEADFLVTLKKIEAQHLPEVNDAFAKSLGIASANVEGLREDIKKNL
                     EREVKFRVIARNKAAVMEALVKTAQLEVPKVLVQNEVERMVANARADLKQRGVKDADK
                     APIPAEMFQPQAEQRVRLGLVVAELVKAEKLHATPEQIKAHVEELAQSYEKPEEVVRW
                     YFSDRQRMAEVEAIVIENNVTNHVLSKAKVTDKQVAFDELMGA"
     gene            6914..7522
                     /gene="clpP"
                     /locus_tag="VFV25_09310"
     CDS             6914..7522
                     /gene="clpP"
                     /locus_tag="VFV25_09310"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEY1393565.1"
                     /translation="MSALETRNLGMVPIVIEQSGRGERAYDIYSRLLRERVIFLVGPV
                     NDQTANLVVAQLLFLESENPDKDISLYINSPGGSVSAGMAIYDTMQFIKPDVSTLCIG
                     MAASMGAFLLAAGAKGKRFALPNSRVMIHQPLGGAQGQATDIEIQAREILKLRENLNK
                     ILAERTGQTLDKIQADTERDFFMSGDEAKGYGLIDQVIARRP"
     gene            7634..8905
                     /gene="clpX"
                     /locus_tag="VFV25_09315"
     CDS             7634..8905
                     /gene="clpX"
                     /locus_tag="VFV25_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEY1393566.1"
                     /translation="MAEKKGSSSEKVLYCSFCGKSQHEVKKLIAGPSVFICDECIELC
                     NDIIRDEVPAEGADAKAARSDLPVPGEIKGILDQYVIGQEAAKRTLSVAVYNHYKRLK
                     HMGGGKKDDVELAKSNILLIGPTGSGKTLLAQTLARLLNVPFVIADATTLTEAGYVGE
                     DVENIIQKLLQNCNYDVEKAQRGIVYIDEIDKISRKADNPSITRDVSGEGVQQALLKL
                     VEGTMASVPPQGGRKHPNQDFLQIDTTNILFICGGAFDGLEKVIQNRTEKSGIGFAAT
                     VHSKSEKRAADLFREAEPEDLIKFGLIPELVGRLPVVATLGELTEEALVRILTEPKNA
                     LVKQYQKLFAMDGVELEVRPSALAAIARKALARKTGARGLRSIMEGSLIDTMFDLPRL
                     DGVAKVVLDEHNIDEDAKPLLVYREQAKASA"
BASE COUNT         1647 a         2907 c         3034 g         1428 t
ORIGIN      
        1 cgacgtcttc gaccagggcg cgcgcggcag ggctgtcgtg ccgggcgagc agggctgccg
       61 gttcgacgcc atggcgcgcg caatcggcca gcggcagata gagccggccg cggtccaggt
      121 cgatgctcaa gtcctgccag aaattgatca gttgcagcgc gctgcagatg gcgtcggatt
      181 cgtgcagcgc ctgggcttgc gtgacgccgt agaggtgcag cagcaggcgg ccgaccgggt
      241 tggccgagcg gcggcaatag tccagcagtt cttcacggtc ggcgtagcgg gtcttgacca
      301 catcctgctc gaaggcgctg agcaggtctt ccatcagcgg caaaggcagc ccgaattcgc
      361 gcagcgcggg gcccagggga gcgaagacct cgggccagcg ggagggctgg gcgccgttgg
      421 cagcgccttg cagggccgcg cggtagtcgg ccaggtcggc caggcgctgc gccggtgccg
      481 catcgccctc gtcggcaatg tcgtcggcgg tgcgcgcgta ccaataaagg gcggcaacgg
      541 ccggcctcag cctgggcggg cacaacagcg atgcgacagg aaagttctcg tagtgatcga
      601 cggacattgc gcgagggcag gcgaactgcc ggacggtgcg cgattgtcca tgcgccgcct
      661 gcttcgccgg tctcctgtac gtttttgcgt ttgacagccc ggggggaccc gaatatatta
      721 ctgaccagtc agtcagttat tttcagaaag catcgcgtcg ggcgcgctgt gccgccgaga
      781 ggaagcggta gcgcagtctt ccggggcgcc agaacaagcc gcgaagcatc cgattccgca
      841 ccggcataca gcatcgccga tgcccctagt ccgaggaaac cccatgagcc cttcgcccct
      901 cgccgccttt tccgcccggc ccgccagccg cgttgccgcc ctggccatcg tcgcagccgg
      961 cgccctgctg gcggcctgct ccaagagcga tccgccgccc gagccggtgc gtgccgtacg
     1021 caccatgcag atcgccagcg aagccgccgg caacacgctg gaatacgccg ccgaggtcaa
     1081 ggcccgggtc gaatcccggc tgggttttcg cgtgggcggc aagctgatgc gccgcgccgt
     1141 caatctgggc gacacggtca aggcagggca gctgttggcg caactcgatg cccgcgatct
     1201 ggtgcttggc gagcaggccg ccaaggcgaa tctgtcggcg gcccaggtca gcttcgatct
     1261 ggcccaggcc gacttcaagc gctacaagga tctgcgcgac caggacttca tcggcgcggc
     1321 cgagctcgac cgccgcgagg cgacgctgaa atccgccaag gccgcgctgg agcaggctag
     1381 ggcccaggcc aatgtgcagg gcaaccaggc gggctatgcc gatctgttgg ccgatgcggc
     1441 gggcgtgatc accggggtcg atgccgagcc gggccaggtc gtcgcggccg gtactccggt
     1501 actgcgcctg gcccaggatg ggccgcgcga tgtcgtgttc tcggtgcccg aggacaaggt
     1561 gcagaacgcg cgcgccctgg tcggcaaggc cggcgggctc aaggtcaagc tctggggcag
     1621 caatgagcaa ctgcccgcca tggtgcgcga gatcgcggcg gcggcggatc ctgccacgcg
     1681 caccttcctc gtcaaggccg acgtcggccg caacaccgtg aagctgggcc agacggccac
     1741 ggtctcgctc gacctgccca ggaccgacgg catcaccaag ctgccgctca cggccttgat
     1801 ggagcagcag ggcaagacct cggtgtggct gctcgacaag gcgagcatga cggtaaaacc
     1861 gcaagtcgtg caggtgtcgg gcgccgaagg caacgcggcc atcgtgacgg cgggcttgag
     1921 cccgggtcag ctggtggtga ccggcggcgt gcatgtgctg acgccggggc agaaggtcaa
     1981 gctctatggc gtggccgatg ccaccaatga tgccgcggcc gcgcaggctg ctaccgaagc
     2041 tatgcgctga tctgggcccg ggacactcac gatgacgcaa gaacaagctc aaaagagcgc
     2101 tgcccagccg cagcgcttca acatctcccg gtgggcactc gagcacgcgc cgctgacgcg
     2161 ctacctgatg gtggtgctga tggtgctggg cattgccgcc tatttccagc tggggcaaga
     2221 cgaggacccg cccttcacct tccgcgccat ggtggtgagc gcctattggc cgggcgccac
     2281 ggcgcagcag atggccgagc aggtgaccga caagatcgag cgcacgctgc aggaggcgcc
     2341 ctatgcggac aagatccgca gctacaccaa gccgggcgag tctctgacca tcttccagct
     2401 caaggactcc tcgccgccca aggaggtctc caatgcctgg taccaggtgc gcaagaagat
     2461 cggcgatatg cgctcgacgc tgccgcaagg cgtgctgggt ccggtcttca acgacgagtt
     2521 cggcgacgtc tacggctcca tctatgcgct gagcgccgac ggcttcagcg cggaggagct
     2581 gcgccagttc gccgagcagg tgcgctcgcg cctgctgcac gtgcccgacg tggccaaggt
     2641 gcagctcttc ggtgtgcagg acgagaagat cttcatcgag atctcgcaga agcgcctggc
     2701 ccagctcggc atcgacttca accaagtgat cgcccagctc ggcgcgcaga acgccgtgga
     2761 aggcgccggc gtgctcgatg cggccggcga caacgtgcag gtgcgcgttg gcggccagtt
     2821 cgattcggtg gaagacctaa gggccttccc gatccgcgcc gtcgacccgg cgacaggcca
     2881 agccagctcg ctgcgcctgg gcgatatcgc cgacatcaag cgctcctaca tcgatccgcc
     2941 ttcggtcaag gtgcgccacc agggcaagga ggtcgtcgcc atgggtatct ccatggccaa
     3001 gggcggcgac atcatcgagc tgggcaagca tctgcacgag accgtggcca tgatccagaa
     3061 agatctgccc gtcggcgtgg agatgagcca gatccaggac cagccgcgcg ccgtttcgcg
     3121 ctcggtgggc gagttcgtgg acgtgctggc ggaggccatt gtggtggtgc tggcggtcag
     3181 cttcatcagc ctcggcttgc attccagcac cgggcgtctg ggctttcgca ttgacatctg
     3241 gcccggcatg gtggtcgcca tcacgatccc gctggtgctg gcgatcacct tcgtcaccat
     3301 gtattactgg ggtgtgggcc tgcacaagat ctcgctgggc gccctgatca tcgcgctggg
     3361 cctgctggtc gacgacgcga tcatctcggt cgagatgatg gtgcgcaagc tcgaagaggg
     3421 ctacgacaag aagcgcgccg ccaccttcgc ctacgagctc acggccatgc ccatgctgac
     3481 cggcacgctg atcacggcgg ccggcttcct tccgatcggc atcgccaagt cgaccgtggg
     3541 cgaatacacc ttcgcgatct tcgccgtgac tacggcggcg ctgctgatct cgtggctggt
     3601 gtcggtgtat ttcgtgccgt atctgggcac cttgttgctg cacaccagag ccaaggtgca
     3661 gggcgagacg catgagctct tcgacacgcc tttctacaac cgcttccgcg cgctggtgaa
     3721 ctggtgcgtg gagcaccgct ggaagacgat aggcgcgacg ctggcgctgt tcgtgctggg
     3781 gctcgtcggc atgggcaagg tgcagcagca gttcttcccc gattccagcc ggccggagat
     3841 cctggtcgac ctctggctac ccgaaggctc gggctatgtc accaccgagg ccatcgccaa
     3901 gcgcttcgaa gcgcgcatga tgaaggagcc cggtgttgcc accgtcagca cctgggtggg
     3961 cagcggcgtg ccgcgcttct atttgccgtt ggaccagatc ttcccgcaga ccaatgtcat
     4021 gcaggccatc ctgttgccca aggacctgaa gacgcgcgag gagctgcgcc agcgcctgcc
     4081 ggcgctgctg gccgaggaat tcccggatgt gcgcggccgc gtgaagctcc tgcccaacgg
     4141 gccgccggtg ccctatccgg tgcagttccg cgtcatcggc agcgacgtca agcaggtgcg
     4201 tacctgggcc gacgaggcca aggcggtgct gcgcgagaac cccaacatgc gcggcgtcaa
     4261 cgacaactgg aacgaatcgg tcaaggtcct gaggctggag atcgaccagg acaaggcgcg
     4321 cgccctgggc gtcaccagcc aggccatcgc gcaggcttcg cgcactatcc tatcgggcac
     4381 caacatcggc cagtaccgcc agggcgaccg tctgctcgac atcgtgctca ggcagccgct
     4441 ggaggagcgc aaggccatca ccgacatcgg caatgcctat gtgccgacct ccaccggccg
     4501 ggccatccct ctgacgcaga tcgccaaggt cggcttcacc tgggagcccg gcgtgctgtg
     4561 gcgcgagggc cgcaactacg cggtgacggt gcagggcgac gtggtcgagg gcatgcaagg
     4621 cgccacggtg acggcgcaga tctggcccaa gctgcagcag ctggtgcagc gcatgccgcc
     4681 cggctattcc atcgaggtgg cgggtgcggt tgaggagagc agcaagggcc agggctcgat
     4741 cgccgtgggc gcgccgctga tgctcttcat catgtttacg ctgctgatgc tgcagctgca
     4801 tagcgtgagc cgcgcggtgc tggtcttcct caccgggccg atgggcatcg cgggcgtcgc
     4861 cggcgccttg ctggtgctga accggccctt cggcttcgtc gcgctgctgg gcgtgatcgc
     4921 gctgatgggc atgatcatgc gcaactcggt gatcctgatc gaccagatcg agcaggaccg
     4981 cgccaagggc gtgccgacct ggaacgccat cgtcgaatcg gcggtgcggc gcttccggcc
     5041 catcgtgctg acggctgcgg cggcggtgtt ggcgatgatc ccgctgtcgc gcagcgtgtt
     5101 ctgggggccg atggcggtgg ccatcatggg cggcctgatc gtcgcgacgg cgctgacgct
     5161 gctggccttg ccggcgatgt atgcggcctg gttccgcgtg aaaagagagg tgccgaaaga
     5221 ggcgcccgcc ccagccgcaa ttccggccga gggccatgcc ctgatgcccc aggaaagtcc
     5281 caggcccgcg acaagctaaa atcaaccgtt taccggatca cccgagggga gccgaagcgc
     5341 tcccctcgcc atttccagcg cgggtggcga aattggtaga cgcaccaggt ttaggtcctg
     5401 acgccagcga tggtgtgggg gttcgagtcc ccccccgcgc accagacgac aagcaactag
     5461 ttggatagcg agagatagaa atggccgtca ctgttgaaaa cctcgaaaag ctcgaacgcc
     5521 gcatcacctt gaccctgtcg gccgatgtgc tgaggagcga ggtcgaatcg cgcctgaagc
     5581 gcctggcgcg caccgtcaag gccgacggct tccgccccgg caaggtgccg atgagcgtcg
     5641 tcgcccagcg ctatggctac tcggtccagt acgaggtgat gaacgacaag gtcggcgagg
     5701 ccttcagcaa ggccgccacc gaggccgagc tgcgcgtggc cggtgcgccg cgcatcaccg
     5761 agaaggaagg cgcgccggcg ggcgaactgg ctttcgatgc caccttcgag gtctatcccg
     5821 aagtcaagct cggcgacctg tcggccgccg agattgagcg tgtcggcacc gaagtgaccg
     5881 aggccgcgat cgacaagacg ctggacatcc tgcgcaagca gcgccgtacc ttcgcccaac
     5941 gccccgcggc cgaaggcgcc gccgacggcg accgcgtgac gatcgacttc gaaggcaaga
     6001 tcgacggcac gcctttcgaa ggcggcaagg cagacggctt ccagttcatc ctgggcgagg
     6061 gccagatgct ggaggccttc gagaaggccg tgcgcggcat gaaggcgggc gagtccaaga
     6121 ccttcccgct ggccttcccg gaggactacc acggcaagga cgtggcgggc aaagaagccg
     6181 atttcctcgt cacgctcaag aagatcgagg cccagcatct gcccgaggtc aacgatgcct
     6241 tcgccaagtc gctgggcatt gccagcgcca atgtcgaagg cctgcgcgag gacatcaaga
     6301 agaacctcga gcgcgaagtc aagttccgcg tcatcgcgcg caacaaggcg gccgtgatgg
     6361 aagccctggt caagacggcc cagctcgaag tccccaaggt tctggtgcag aacgaagtgg
     6421 agcgcatggt cgccaatgcc cgtgccgacc tcaagcagcg cggcgtcaag gacgccgaca
     6481 aggcgccgat ccccgccgag atgttccagc cccaggccga gcagcgcgtg cgcctgggcc
     6541 tggtggtggc cgagctggtg aaggccgaga agctgcacgc cacgcccgag cagatcaagg
     6601 cccatgtcga ggagctggcg cagagctatg agaagcccga agaggtggtg cgctggtact
     6661 tcagcgaccg ccagcgcatg gccgaggtcg aggcgatcgt gatcgagaac aatgtcacga
     6721 accatgtgct ttccaaggcc aaggtcacgg acaagcaggt cgccttcgac gagctgatgg
     6781 gcgcctgagc cttcgagcct gatgccaagg cgccgtgccg cgaggccggc gccttgtcgt
     6841 ttcggcaggg ctccatcttg acgctacata atcgaacccc agatagcacg caacgacatc
     6901 cttgaggaga gccatgagcg ctttggagac ccgaaacctg ggcatggtgc ccatcgtcat
     6961 cgagcaatcg ggccggggcg aacgcgccta cgacatctac agccgcctgc tgcgcgagcg
     7021 ggtgatcttc ctggtcggtc cggtcaacga tcagaccgcc aacctggtcg tggcccagct
     7081 gctgttcctg gagagtgaga acccggacaa ggacatctcg ctgtacatca attcgcccgg
     7141 cggcagcgtc agcgccggca tggcgatcta cgacaccatg cagttcatca aacccgacgt
     7201 ctcgacgctg tgcatcggca tggcggccag catgggtgcc ttcctgctgg cggccggcgc
     7261 caagggcaag cgcttcgcgt tgccgaattc gcgcgtcatg atccaccagc cgctgggcgg
     7321 tgcccagggc caggcgaccg acatcgagat ccaggcgcgc gagatcctga agctgcgcga
     7381 gaacctaaac aagatccttg ccgagcgcac cggccagacg ctggacaaga tccaggccga
     7441 caccgagcgc gacttcttca tgtcgggcga cgaggccaag ggctacggcc tgatcgacca
     7501 ggtcatcgcc aggcggccct gaggcccgcc gagcccggca aatctgggcg gccaacgggg
     7561 cctctttttc gagggatggc cctggtttct tttgccttat catcctcagc gttccctccc
     7621 tgttttttca cccatggccg agaaaaaagg ctcctccagc gaaaaggtct tgtactgctc
     7681 gttctgcggc aagagtcagc acgaggtcaa gaagctcatc gccggcccgt cggtgttcat
     7741 ctgcgacgag tgcatcgagc tttgcaacga catcattcgt gacgaagttc cggccgaggg
     7801 cgcagacgcc aaggccgcgc gcagcgatct gccggtgccg ggcgagatca agggcatcct
     7861 cgaccagtac gtgatcggcc aggaggcggc caagcggacg ctgtcggtgg ccgtctacaa
     7921 ccactacaag cgcctgaagc acatgggcgg cggcaagaag gacgatgtcg agctggccaa
     7981 gagcaacatc ctcctgatcg gccccacggg ctcgggcaag accctgctgg cacagacgct
     8041 ggcgaggctc ttgaacgtgc ccttcgtgat cgccgacgcg accaccttga ccgaggccgg
     8101 ctacgtgggc gaggacgtcg agaacatcat ccagaagctg ctgcagaact gcaactacga
     8161 cgtcgagaag gcgcagcgcg gcatcgtcta catcgacgag atcgacaaga tctcgcgcaa
     8221 ggccgacaac ccctcgatca cgcgcgacgt atccggcgag ggcgtgcagc aggcgctgct
     8281 caagctggtg gaaggcacga tggcctcggt gccgccccag ggcgggcgca agcatccgaa
     8341 tcaggacttc ctgcagatcg acacgaccaa catcctgttc atctgcggcg gcgccttcga
     8401 cgggctggag aaggtgatcc aaaaccgcac cgagaagtcg ggcatcggtt ttgctgccac
     8461 cgtgcacagc aagagcgaga agcgcgccgc cgacctgttc cgcgaggccg agccggaaga
     8521 cctcatcaag ttcggcctga tccccgagct ggtcggccgc ctgccggtgg tggcgacgct
     8581 gggcgagctg accgaggagg cgctggtgcg catcctcacc gagccgaaga acgcgctagt
     8641 caagcaatac cagaaactgt tcgcgatgga cggggtcgaa ctcgaagttc gcccgtcggc
     8701 gctggcggcg atcgcgcgca aggcgttggc gcgcaagacc ggcgcgcgcg ggctgcgctc
     8761 gatcatggaa ggttcgctga tcgacacgat gttcgacctg ccgcgcctgg atggcgtggc
     8821 caaggtcgtg ctggacgagc acaacatcga cgaagacgcc aagcccttgc tggtgtatcg
     8881 cgagcaggcc aaggccagcg cttgagagcg ttctcgcggc gctaggcagg cgccttgcaa
     8941 acaatggctc gggccccaca tcggttcgag agagaaaggt aaccctcatg tccggacatc
     9001 ccatcctgcc cgccga
//
LOCUS       DASUCY010000467         2208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1584465, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000467 DASUCY010000000
VERSION     DASUCY010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1584465"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VFV25_09320"
     CDS             <1..624
                     /locus_tag="VFV25_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY1393567.1"
                     /translation="GNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIR
                     IPVHMIETINKMNRISRQHLQEFGFEPDAPTLAEKMEMPEDKVRKIMKIAKEPISMET
                     PIGDDDDSHLGDFIEDTNNTAPIEAAMQAGLRDVVKDILDSLTPREAKVLRMRFGIEM
                     STDHTLEEVGKQFDVTRERIRQIEAKAIRKLKHPSRSDKLRTYLDNL"
     gene            753..1688
                     /locus_tag="VFV25_09325"
     CDS             753..1688
                     /locus_tag="VFV25_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEY1393568.1"
                     /translation="MTTPVVRTVLFADLRGSTSLYETLGNAEATAVVTQSVALLAQVV
                     INCNGRVIKTLGDGLMALFEHAQQGLQAADEMHESLERIASLPSVPGRAPALKLQVAL
                     ALGEVIELAGDCFGDAVNVAARLLDHAGDNETLVTGNVLANADGSQLGRVRRLDRMQL
                     RGRLEPVEVHRMEGRRFNDTQVTAFQDVPPVMVPDGIRLVWLDLNRVFAGASLPVILG
                     RSSQATYCVDDSRVSRSHARIEWHGGNFQLTDLSYNGTYVRYGNQADIVALKRSTCVL
                     HGRGLIGLGASPADASAPCVRFEVLSFSDTVPHVP"
     gene            complement(1771..>2208)
                     /locus_tag="VFV25_09330"
     CDS             complement(1771..>2208)
                     /locus_tag="VFV25_09330"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020424688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY1393569.1"
                     /translation="GDDPALAEPAFECFFAERQKVDLFDDVLPALERLASRYPLLALS
                     NGNADIARIGLARYFRGSLSAREFGVGKPDPRIFHAACGRLGQPGASVLHVGDDPLLD
                     IAAAHAAGQPSAWVKRPGALHEKPAQATHAVENLLQLADLLGA"
BASE COUNT          393 a          769 c          717 g          329 t
ORIGIN      
        1 ggcaacatcg gcctgatgaa ggcggtggac aagttcgaat accgccgcgg ctacaagttc
       61 tcgacctacg ccacgtggtg gatccgccag gccatcacgc gctcgatcgc cgaccaggcg
      121 cgcaccatcc gcattccggt gcacatgatc gagacgatca acaagatgaa ccgcatctcg
      181 cgccagcacc tgcaggagtt cggcttcgag ccggacgcgc cgacgctggc cgagaagatg
      241 gagatgcccg aggacaaggt gcgcaagatc atgaagatcg ccaaggagcc gatctccatg
      301 gagacgccga tcggcgacga cgacgattcg cacctgggcg atttcatcga ggacaccaac
      361 aacaccgcac cgatcgaggc tgccatgcag gccggcctgc gcgacgtggt gaaggacatc
      421 ctcgattcgc tgaccccgcg cgaagccaag gtgctgcgca tgcgcttcgg catcgagatg
      481 tccaccgacc acacgctgga agaagtcggc aagcagttcg atgtgacacg cgagcgcatc
      541 cgccagatcg aggccaaggc gatccgcaag ctcaagcacc cgagccgctc ggacaagctc
      601 aggacctacc tggacaatct ctgaaagaga ttgccggggc ccttcggttt cctggtgccg
      661 ctcagtgaaa aagcccggct ttgccgggct ttttcatgcc tcgcgggcgc acgcagaggg
      721 gactacactg cggaacaacg cgcctcccac agatgaccac tcccgtcgtc cgcaccgtcc
      781 tcttcgccga tctgcgcggc agcacctcgc tgtacgagac gctgggcaat gccgaggcaa
      841 ccgccgtcgt cacgcagagc gtggccctgc tggcccaggt ggtgatcaac tgcaacggcc
      901 gcgtgatcaa gaccctgggc gacggcctga tggccttgtt cgagcacgcc cagcagggtt
      961 tgcaggcagc cgacgaaatg catgaatcgc tggaacgcat cgccagcctg ccctccgtgc
     1021 ccgggcgcgc gccggcgctc aagctgcagg tcgccctggc gctcggcgag gtgatcgagc
     1081 tggcgggcga ctgcttcggc gacgccgtca atgtcgcagc gcggctgctc gaccatgccg
     1141 gggacaacga gaccctggtc accggcaatg tgctggccaa cgccgacggc agccagctgg
     1201 gccgcgtgcg ccgcctcgac aggatgcagc tgcgcggccg cctggagccg gtcgaagtgc
     1261 atcgcatgga ggggcgccgc ttcaacgaca cccaggtcac cgccttccag gacgtgccgc
     1321 cggtcatggt gccggacggg atacgcctgg tgtggctgga tctgaaccgc gtctttgccg
     1381 gcgccagcct ccccgtgatc ctgggccgca gctcgcaggc cacttattgt gtcgacgact
     1441 cccgggtgtc gcgctcgcat gcccgcatcg aatggcatgg cggcaacttc cagctcaccg
     1501 acctcagcta caacggcacc tatgtccgct acggcaacca ggccgacatc gtggcgctca
     1561 agcgcagcac ctgcgtgctg cacgggcgcg gcctgatcgg gctgggcgcc tcgcctgccg
     1621 atgcctccgc gccctgcgtg cgcttcgagg tgctgtcctt ctcggacacc gtccctcacg
     1681 tgccttgagc gcccccaggg ccgggctagg cccagcccgc cccccgaggg ggtcaaggaa
     1741 cttggggcgg cccggcgctc cttgaggcgc tcaggcgccc agcaggtcgg ccagctgcag
     1801 gaggttctcc acggcatgcg tcgcctgcgc cggcttctcg tgcagcgcgc cgggccgctt
     1861 gacccaggcc gaaggctggc cagcggcatg ggctgcagcg atgtccagca gcgggtcgtc
     1921 gcccacatgc agcaccgatg cacccggctg acccaagcgc ccgcaagcgg catgaaagat
     1981 gcgcggatcg ggcttgccga cgccgaattc gcgcgcgctg aggctgccgc ggaaatagcg
     2041 cgcgaggccg atgcgtgcga tgtccgcatt gccgttgctc agcgccagca gcggatagcg
     2101 gctcgccagg cgctccagcg ccggcagcac atcatcgaac aagtcgacct tctggcgctc
     2161 ggcgaagaag cattcgaagg ccggctccgc cagagccggg tcatcgcc
//
LOCUS       DASUCY010000468         4049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_58697, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000468 DASUCY010000000
VERSION     DASUCY010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58697"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /gene="glk"
                     /locus_tag="VFV25_09335"
     CDS             <1..869
                     /gene="glk"
                     /locus_tag="VFV25_09335"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEY1393570.1"
                     /translation="NYSCADHASLADAMQRYLDEHGGRPRWGAIGLANPVTGDQVRMT
                     NSHWVFSISETQRRLGLTRFVVINDLTALALALPALSPAELKPIGGGQAVAGAPLGVV
                     GPGTGLGVAGLLPATAGRGAIPISSEGGHATLAPEDAQEEAVIRVLRERYGHASAERA
                     LSGAGLVNLYQAVCVVQGVRGREIQAADVTTLALSGEDAQCVAALDLFCALLGSVAGN
                     VALMLCARGGIYIGGGIVPRLGAAFERSRFRERFEAKGQLHDYVREIPTYVIQAQVSP
                     ALLGAARALEEL"
     gene            complement(921..1706)
                     /gene="fabI"
                     /locus_tag="VFV25_09340"
     CDS             complement(921..1706)
                     /gene="fabI"
                     /locus_tag="VFV25_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEY1393571.1"
                     /translation="MGFLAGKRLLITGLLSNRSIAYGIAKACKREGAELAFSYVGERF
                     KDRITDFAREFGSELVFDCDVGDDAQIQALFADLAKTWPQFDGFVHSIGFAPREAIAG
                     DFLDGLTREAFRIAHDISAYSFPAMAKAAATMLTPNAALLTLSYLGSVRVVPHYNTMG
                     LAKASLEASVRYLAASLGSRGVRVNGISAGPIKTLAASGIKDFGKMLAAVEQASPLKR
                     NVTIDDVGNVAAFLLSDLAAGVTAEITYVDAGFSQMMGGVAEG"
     gene            1942..2493
                     /gene="rimP"
                     /locus_tag="VFV25_09345"
     CDS             1942..2493
                     /gene="rimP"
                     /locus_tag="VFV25_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEY1393572.1"
                     /translation="MNWHAAVERTVSGLGYDLVDCERSSQGLLRVFIDRLPGQAYEAH
                     GMGPGEFVTVDDCERVTRQLQYVLEVEGCDYSRLEVSSPGLDRPLKKPGDYERFAGLE
                     VDITLKLPFQGRKKYRGVLSAAGEAWQLAYHDGKEDKALGFTLDEVREARLVPVVDFK
                     GRARKSAPEAAAGRAEEIGGHEE"
     gene            2490..3968
                     /gene="nusA"
                     /locus_tag="VFV25_09350"
     CDS             2490..3968
                     /gene="nusA"
                     /locus_tag="VFV25_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEY1393573.1"
                     /translation="MNRELLMLVDAISREKSVDREVVFGAVEAALASATKKLHGGEVD
                     IRVSVDRETGVYETFRRWLVVPDSAGLQNPDAEEILSDAQERIGDIEEGDYIEEPIES
                     VTIGRIGAQAAKQVILQKIRDAEREQLLNDFLSRGDKIFVGTVKRLDKGDIIVESGRV
                     EGRLKRSEMISKENLRSGDRVRAYIAEVDPTVRGPQIMLSRSSPGFMIELFRQEVPEI
                     EQGLLEIKSCARDAGSRAKIAVLSHDKRVDPIGTCVGVRGSRVNAVTNELAGERVDIV
                     LWSEDPAQFVIGALAPANVQSIVVDEEKHAMDVVVDEENLAIAIGRGGQNVRLASELT
                     GWRINIMTAEESQAKQATESDSIRKLFVDKLDVDQEVADILIEEGFTSLEEVAYVPLQ
                     EMLEIESFDEDTVNELRTRAKDALLTMEIAKEEKVDEVSQDLRDLEGLNGELIAKLSD
                     AGIHTRDDLADLAVDELTEISGVGVEQAKALIMKAREHWFTA"
BASE COUNT          717 a         1272 c         1419 g          641 t
ORIGIN      
        1 cgaactacag ctgcgccgac cacgcctcgc tggcggacgc gatgcagcgc tacctggacg
       61 agcatggcgg ccgtccgcgc tggggcgcga tcggcctggc caatcccgtg acgggcgacc
      121 aggtgcgcat gaccaacagc cactgggtgt tctcgatcag cgagacccag cgccgcctgg
      181 gcctcacgcg tttcgtcgtc atcaacgact tgacggcgct ggccctggcg ctgccggcgc
      241 tgagccccgc ggagctcaag cccatcggcg gcggccaggc ggtggccggc gcgccgctgg
      301 gtgtcgtcgg gccgggcacc ggcctgggcg tggcggggct gttgccggcc acggcaggcc
      361 gtggggcgat tcccatcagc agcgaaggcg gccatgccac gctggcgccg gaggatgccc
      421 aggaagaggc cgtgatccgg gtgctgcgcg agcgctatgg ccatgcctcg gccgagcggg
      481 cgctgtccgg cgccgggctg gtcaacctct accaggcggt ttgcgtcgtg cagggggtcc
      541 gggggcgcga gatccaggcc gcggacgtga cgaccctggc cttgtccggc gaggatgcgc
      601 agtgcgtcgc ggcgctcgat ctcttctgcg ccctgctcgg cagcgtggcc ggaaatgtgg
      661 cgctgatgct gtgcgcgcgc ggcggcatct acatcggggg cggcatcgtg ccccggctgg
      721 gcgcggcctt cgagcgttcg cgctttcgcg agcgcttcga ggccaagggg cagctgcacg
      781 actatgtgcg cgagatcccg acctatgtca tccaggccca ggtctcgccg gccttgctgg
      841 gtgcggcgcg ggcgctggaa gagctctgaa gacgacgagg cccgctgttc gcgggcctcg
      901 ctgccggtgt ccggaggaac tcagccctcg gcgacgccgc ccatcatctg gctgaatccg
      961 gcgtccacat aggtgatctc ggccgtcacg ccggcggcaa ggtccgacag caggaaggcg
     1021 gcaacattgc ccacgtcgtc gatcgtgacg ttgcgcttga gcggcgaggc ctgctccacc
     1081 gcggcgagca tcttgccgaa gtccttgatg cccgaggccg ccagcgtctt gatcggcccg
     1141 gccgaaatgc cgttgacgcg cacgcccctg gagcccaggc tggccgccag gtagcgcacg
     1201 ctggcctcca gcgaggcctt ggccaggccc atggtgttgt agtgcggcac gacccgcacc
     1261 gagccgaggt agctcagggt cagcagggcg gcattgggcg tgagcatagt cgcggccgcc
     1321 ttggccatgg ccgggaagct gtaggcggag atgtcgtggg cgatgcggaa ggcctcgcgc
     1381 gtcaggccgt caagaaaatc gccggcgatc gcctcgcgcg gcgcgaagcc gatggaatgc
     1441 acgaaaccgt cgaactgcgg ccaggtcttg gccagatcgg cgaacagggc ctggatctgg
     1501 gcgtcgtcgc ccacgtcgca gtcgaacacc agctcggagc cgaattcccg ggcgaaatcg
     1561 gtgatgcggt ccttgaagcg ctcgccgaca tagctgaagg ccagctcggc cccctcccgc
     1621 ttgcaggcct tggcgatgcc ataggcaatc gagcggttgg acagcaaacc ggtaatcagc
     1681 aggcgcttgc cggcgagaaa gcccatggga aagactccgt acgatttcga cagcctgaga
     1741 ttctggcacg cgggggctcg ccgaggcctg cttgacagag gtctgacttg cctgtagcgg
     1801 cccggcgcgc tacaatgcgc ctttcgctag gcaatcgatt gggcggggaa atccagccca
     1861 ttttttttgc cgtttagctt tttgctgcgg cattccttgg tatttcctgt aaggccgacc
     1921 gagtatccat agatggcatt gatgaattgg cacgccgccg tcgaacgcac agtctccgga
     1981 ctgggctacg acctggtcga ttgcgagcgc agctcgcaag ggctgctgcg cgtcttcatc
     2041 gaccggttgc cgggtcaggc ctacgaggcc catggaatgg ggccgggcga gttcgtcacg
     2101 gtggacgatt gcgagagggt cacgcgccag ctgcaatatg tgctcgaagt ggaaggctgc
     2161 gactacagcc gcctggaggt ctcttccccg ggcctcgacc ggccgctgaa gaagcctggc
     2221 gactacgagc gctttgccgg cctggaggtc gacatcacgc tgaagctgcc cttccagggc
     2281 cgcaagaagt accgcggtgt gctgagcgcc gccggcgagg cctggcagct tgcctatcac
     2341 gacggcaagg aagacaaggc attgggattc acgctcgacg aagtccgcga ggcccggctg
     2401 gtgccggtgg tggacttcaa ggggcgcgcc cgcaaatcgg cgcctgaagc ggccgcgggc
     2461 agagcagagg aaatcggagg tcacgaagaa tgaaccgcga actgttgatg ctggtggatg
     2521 cgatctcgcg cgagaagagc gtggatcgcg aggtggtttt cggcgccgtc gaggcggcgc
     2581 tggcctccgc caccaagaag ctgcatggcg gcgaggtcga catccgcgta tcggtcgacc
     2641 gcgagaccgg cgtctacgag accttccgcc gctggctggt ggtgcccgat tccgccgggc
     2701 tgcagaaccc cgacgccgag gagatcctga gcgacgcgca ggagcgcatc ggcgacatcg
     2761 aggagggcga ctacatcgag gagcccatcg agtcggtgac gatcggccgc atcggcgccc
     2821 aggccgccaa gcaggtgatc ctgcagaaga tccgcgatgc cgagcgcgag cagctgctca
     2881 acgacttcct gtcgcgcggc gacaagatct tcgtcggcac ggtcaagcgc ctggacaagg
     2941 gcgacatcat tgtcgagagc ggccgcgtcg aagggcggct caagcgcagc gagatgatct
     3001 ccaaggagaa cctgcgcagc ggcgaccgcg tgcgcgccta catcgccgag gtcgacccga
     3061 ccgtgcgcgg cccgcagatc atgctgtcgc gcagctcgcc gggcttcatg atcgagctgt
     3121 tccgccagga ggtgcccgag atcgagcagg gcctgctgga gatcaagagc tgcgcccgcg
     3181 atgccggctc gcgcgccaag atcgccgtgc tctcgcacga caagcgcgtc gacccgatcg
     3241 gcacctgcgt gggcgtgcgc ggctcgcgcg tcaacgccgt gaccaacgag ctggctggcg
     3301 agcgtgttga tatcgtgctg tggtccgagg atccggccca attcgtgatc ggcgcgctgg
     3361 cgccggccaa cgtgcagtcc atcgtggtcg atgaagagaa gcacgcgatg gacgtggtgg
     3421 tcgacgagga aaacctcgcc atcgccatcg gtcgtggcgg ccagaacgta cgcttggctt
     3481 cggagctgac cggctggcgc atcaacatca tgacggccga ggaatcgcag gccaagcagg
     3541 cgaccgagag cgattcgatc cgcaagctct tcgtcgacaa gctcgatgtg gaccaggaag
     3601 tcgccgatat cctgatcgag gaaggcttca ccagcctgga agaagtggcc tacgtgccgc
     3661 tgcaggaaat gttggagatc gagagtttcg acgaggacac tgtcaacgag ctgcgcacgc
     3721 gagccaaaga tgcgctgctg accatggaaa tcgccaagga agaaaaggtc gacgaggtgt
     3781 cgcaggatct gcgcgatctg gaaggcctga acggcgagtt gattgccaaa ctgtccgatg
     3841 ccggcatcca tacccgtgac gacctagccg atctggcggt cgacgagctg accgagattt
     3901 ctggagtagg cgtggagcag gccaaggcgc tgatcatgaa ggcccgcgaa cactggttca
     3961 ctgcctgatc gccacgcagc agcccgactc gcagacgccc aagaacgcat accgcaagga
     4021 ctacacacaa tggccgtgac caccgtcgc
//
LOCUS       DASUCY010000469         2498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_508629, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000469 DASUCY010000000
VERSION     DASUCY010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508629"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1533)
                     /gene="ilvD"
                     /locus_tag="VFV25_09355"
     CDS             complement(<1..1533)
                     /gene="ilvD"
                     /locus_tag="VFV25_09355"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEY1393574.1"
                     /translation="MSLNRRSRNITEGVARAPNRSMYYGMGYKDEDFGKAMIGVANGH
                     STITPCNSGLQRLADAAVEGLKAAGANPQIFGTPTISDGMAMGTEGMKYSLVSREVIA
                     DCVETCVGGQWMDGVIVIGGCDKNMPGGMMGMLRANVPSLYVYGGTILPGKYKGQDLN
                     IVSVFEAVGQFAAGKMSQEDFCEIEHRAIPGSGSCGGMYTANTMSSSFEALGLSLPYS
                     STMANVEDEVIANVKKAAAVLVEAVKKDLKPRDIVTRKSIENAAAVIMATGGSTNAVL
                     HFLAIAHAAGVEWTIDDFERVRRRTPVLCDLKPSGKYLAIDLHRAGGIPQVMKVLLKA
                     GLLHGDCITITGKTVTENLADVPDAPRADQDVIRPIDQAIYAQGHLAILRGNLATEGC
                     VAKITGLKNPVITGPARVFDDEQSALAAIMAGRIKPGDVMVLRYLGPKGGPGMPEMLA
                     PTGALIGQGLGESVGLITDGRFSGGTWGMVVGHVAPEAYEGGTIALVQEGDSVTIDAH
                     KLL"
     gene            1656..>2498
                     /locus_tag="VFV25_09360"
     CDS             1656..>2498
                     /locus_tag="VFV25_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY1393575.1"
                     /translation="MSEAIDLRAWRQFLALAELLHFGRAAQRLHMTQPPLTQAIQQLE
                     RRLGVALFERSRRSVALAPAGSALVAPVRALLAQAEALPALARAADEGQLGQLRLGFV
                     STVGFDPLPRWLSGFRQAQPRVAVSLREATTDVQLEALERGEIDAGFVLHAPGMAPPG
                     YARLALGREPLVIALPETHALAGAARLSVKALLAEPLVIFPREIAPSLHDAVLAFYHR
                     HGATPQIAQEAIQMQTIVNLVSAGLGLAWVPEVITQLQRHGVVYRPLPPALRAGAPHG
                     ETSLI"
BASE COUNT          341 a          848 c          862 g          447 t
ORIGIN      
        1 cagcagcttg tgcgcatcga tggtgacgga gtcgccctcc tgcaccagcg caatcgtgcc
       61 gccttcatag gcctccggcg ccacgtggcc cacaaccatg ccccaggtgc cgcccgagaa
      121 gcggccgtcg gtgatcagcc ccacgctttc gcccaggccc tggccgatca acgccccagt
      181 gggggccagc atctccggca tgcccgggcc gcccttgggg cccaggtagc gcaacaccat
      241 cacatcaccc ggcttgatcc tgccggccat gatggcggcg agcgccgact gctcgtcgtc
      301 gaacacgcgc gccgggccgg tgatgacagg gttcttgagg cccgtgatct tggccacgca
      361 gccttcggtg gccaggttgc cgcgcaggat ggcgaggtgg ccttgggcat agatggcttg
      421 gtcgatcggg cggatcacgt cctggtcggc acgcggagca tcgggcacgt cggccaggtt
      481 ttcggtcacc gtcttgccgg tgatggtgat gcagtcgccg tgcagcaggc cggccttgag
      541 cagcaccttc atcacctgcg ggatgccgcc ggcgcggtgc aggtcgatgg ccaggtactt
      601 gccgctgggc ttcaggtcgc acagcaccgg cgtgcggcgg cgcacgcgct cgaagtcgtc
      661 gatcgtccat tcgacgccgg ccgcatgcgc gatggcgagg aagtgcagca ccgcgttggt
      721 ggagccgccg gtggccatga tcacggccgc ggcgttttcg atggacttgc gcgtcacgat
      781 gtcgcgcggc ttcaggtctt tcttcacggc ttccaccagc acggcggccg ccttcttgac
      841 gttggcgatg acctcgtcct cgacgttggc catggtggag gagtagggca gggacagccc
      901 cagcgcctcg aacgacgagc tcatcgtgtt ggcggtgtac atgccgccgc acgagccgct
      961 gccgggaatg gcgcggtgct cgatctcgca gaagtcttcc tggctcatct tgccggccgc
     1021 gaactggccg acagcctcga acacgctgac gatgttgaga tcctggcctt tgtatttgcc
     1081 cggcaggatc gtgccgccgt agacgtagag gctgggcacg ttggcgcgca gcatgcccat
     1141 catgccgcca ggcatgttct tgtcgcagcc gccgatgacg atcacgccgt ccatccactg
     1201 gccgcccacg caggtctcga cgcagtcggc gatcacctcg cgcgacacca ggctgtactt
     1261 catgccctcg gtgcccatgg ccatgccgtc gctaatggtg ggtgtgccga agatctgcgg
     1321 gttggcgccg gcggccttca ggccctccac ggctgcgtcg gccaggcgct gcaggccgga
     1381 gttgcacggc gtgatggtcg agtggccgtt ggccacgccg atcatcgcct tgccgaagtc
     1441 ttcgtccttg tagcccatgc cgtagtacat ggagcggttg ggcgcgcggg ccacgccttc
     1501 ggtgatgttc ctggagcggc ggttcaggct catgggggtc tccgtggctg gtagtgactg
     1561 gtgactggtg aacagtgact ggaaaagtcc aagtatcgtt atcccgtgct cagcggtcca
     1621 atatatcttt ggcgcccatc tgatacgctg agcatatgag tgaagcgatc gatctgcggg
     1681 cctggcggca gttcctggca ctggccgagt tgctgcattt cggccgtgcc gcccagcgcc
     1741 tgcacatgac ccagccgcca ctgacccagg ccatccagca attggagcgc cgcctgggcg
     1801 tggctttgtt cgagcgcagc cggcgctcgg tggcgctggc gcctgcggga tcggccctgg
     1861 tcgcgccggt gcgggcgctg ctcgcgcagg ccgaggcgct gccggccctg gcgcgtgcgg
     1921 ccgatgaagg ccagctcggc cagctgcggc tgggcttcgt ctccaccgtc ggcttcgatc
     1981 cgctgccgcg ctggctcagc ggctttcgcc aggcccagcc gcgcgttgcc gtgtccttgc
     2041 gcgaggcgac gaccgatgtg cagctcgaag cgctggagcg cggcgagatc gacgccggct
     2101 ttgtgctgca cgcacccggt atggcgccgc ccgggtatgc gcggctggcc ctgggccgcg
     2161 agccgctggt cattgcgctg cccgagaccc acgccctggc cggcgccgcg cggctgagcg
     2221 tgaaggcgct gctggccgag ccgctggtga tcttcccgcg cgagatcgcg ccttcgctgc
     2281 acgacgcggt gctggccttc taccaccgcc acggtgccac gccgcaaatc gcgcaggagg
     2341 ccatccagat gcagaccatc gtcaacctcg tgtcggcggg gctgggcctg gcctgggtgc
     2401 ccgaggtcat cacgcagctg cagcgccacg gcgtcgtcta ccggccgttg ccgccggccc
     2461 tgcgcgccgg cgcgccgcac ggggagacca gcctaatc
//
LOCUS       DASUCY010000470         3812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1022698, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000470 DASUCY010000000
VERSION     DASUCY010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022698"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /gene="istB"
                     /locus_tag="VFV25_09365"
     CDS             <1..655
                     /gene="istB"
                     /locus_tag="VFV25_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEY1393576.1"
                     /translation="WEVEERGRRSLERRLREAHIGQFKPIADFDWRWPTQIDRAAIEE
                     LMTLQFMADATNVVLVGGNGLGKTMFASNLGYQAVLAGHTVLFVSAGQLLGDLAALDS
                     DSTLRRRLRHYAAPDILLIDEVGYLSYSNRHADLLFELVSRRYQHKSTVVTTNKSFAD
                     WGEVFPNAACVVSLVDRLMHRAEVVRIEGESYRAKEAQERLAARARQRSAKPGRSKP"
     gene            652..849
                     /locus_tag="VFV25_09370"
     CDS             652..849
                     /locus_tag="VFV25_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393577.1"
                     /translation="MTARHDEPASLPTHWTPEQALAVFECLHALREQLWSTYGSAVQQ
                     AWCDRLAPRSPWPEVDPDDPF"
     gene            complement(853..1956)
                     /locus_tag="VFV25_09375"
     CDS             complement(853..1956)
                     /locus_tag="VFV25_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEY1393578.1"
                     /translation="MSRTDALQISSTQAGPALSPSQKRFNTLIRQIEQARQTLAAWQD
                     NVAAYRQAHGKVLRPLQAELLAGHRQWVLALDAALEQASWTKAERATLREVLCEAAGE
                     LLAGRGEDEQIRALFDKHSEVDFDTEQRDRMLAMKDMAEAMTGLDLGDDEGIDSDAEL
                     FARLQQGLQEQVAAEEAQRSAKAGRRRKSAAQHKREAEAQQATQSVREIYRKLASALH
                     PDRETDAQQREAKTALMQRVNQAYDAQDLLALLELQLQIEQIDESHIANAGEQRLKHY
                     NKVLGEQLAELKAEVERVEMDFRMEFGLEPGWGMNPRKLGQVLEQTSRQWRAELNEQQ
                     RELRMLADVAATKRWLKRERQRQREAEFDFAPF"
     gene            complement(2068..2403)
                     /locus_tag="VFV25_09380"
     CDS             complement(2068..2403)
                     /locus_tag="VFV25_09380"
                     /inference="COORDINATES: protein motif:HMM:NF024886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEY1393579.1"
                     /translation="MTPLRQRMLDALELRGAAVRTRESYIEAVARLARHYGRSPEALT
                     AEEVQRYLLHLVRERRLARSSVNQYGCAFRFLYGTVLGLDGGAFQIPLAPAPQRLPEI
                     LSREVLAAA"
     gene            2655..2849
                     /locus_tag="VFV25_09385"
     CDS             2655..2849
                     /locus_tag="VFV25_09385"
                     /inference="COORDINATES: protein motif:HMM:NF024886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HEY1393580.1"
                     /translation="MEAVSIPPKQEELALKPRLLDQVRDSLRRRRYSLRTEKVYVHWI
                     RRYILFHGKRHPAEMGARPR"
     gene            2885..3235
                     /locus_tag="VFV25_09390"
                     /pseudo
     CDS             2885..3235
                     /locus_tag="VFV25_09390"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            3363..>3812
                     /locus_tag="VFV25_09395"
     CDS             3363..>3812
                     /locus_tag="VFV25_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol acyltransferase"
                     /protein_id="HEY1393581.1"
                     /translation="MNQAEHPNQFALLTQRRFAPFFWTQFLGAANDNLFKFAFTVLVT
                     YQLSISWLPPELAGLTIGALFILPFLLFSATSGQLADKLEKTRLIRFVKWLEIAIMSL
                     AAWGFVTAQVSALLACVFLMGLHSTIFGPVKYAYLPQHLNERELTGGN"
BASE COUNT          563 a         1323 c         1248 g          678 t
ORIGIN      
        1 ctgggaggtc gaggagcgcg gccggcgcag cctcgagcgg cggctgcgcg aggcgcacat
       61 cgggcagttc aagccgatcg ccgacttcga ctggcgctgg cccacgcaga tcgatcgtgc
      121 ggccatcgag gagctcatga cgctgcagtt catggccgac gcgaccaacg tggtgctggt
      181 cggtggtaac ggtctgggca agaccatgtt cgcgtccaac ctgggctacc aggcggtgct
      241 ggccggccac acggtgctgt tcgtcagtgc cgggcagttg ctgggcgact tggccgccct
      301 ggacagcgac tcgacgctgc gccggcggct gcgtcactac gcagcaccgg acatcttgct
      361 catcgacgag gtggggtacc tctcttactc caaccggcat gccgatctgc tgttcgagct
      421 cgtcagccgt cgctaccagc acaagagcac ggtggtgacg acgaacaagt cgtttgccga
      481 ctggggcgag gtgttcccca atgccgcctg tgtcgtctcg ctggtcgatc gcctcatgca
      541 ccgcgccgag gtcgtgcgca tcgagggtga gtcctaccgc gccaaggaag cgcaggagcg
      601 gctggctgca cgcgccaggc agcgctcggc caagcccggc aggagcaagc cgtgactgcc
      661 cgccacgacg aaccggcgtc gttgccgacg cactggacgc ccgagcaggc actggcggtc
      721 ttcgagtgcc tgcacgcgct gcgcgagcag ctgtggtcga cgtacgggtc cgccgtgcag
      781 caagcctggt gcgatcggct ggcgccgcgc tcgccgtggc ccgaggtcga tccggacgat
      841 cccttctgac cgtcagaacg gcgcgaagtc gaactcggct tcgcgctgtc gctgccgctc
      901 gcgcttgagc cagcgcttgg tcgccgccac gtcggccagc atgcgcagct cgcgttgctg
      961 ctcgttgagc tcggcgcgcc actggcggct ggtctgctcc agcacctggc ccagcttgcg
     1021 cgggttcatg ccccagcccg gctccaggcc gaactccatg cggaagtcca tctcgacgcg
     1081 ctccacttcg gccttgagtt cggccagttg ctcgccgagc accttgttgt agtgcttcag
     1141 ccgctgctcg ccggcgttgg cgatgtggct ctcgtcgatc tgctcgatct gcagctgcag
     1201 ttccagcagc gccagcaggt cctgcgcatc gtaggcctgg ttcacccgct gcatcagcgc
     1261 ggtcttggct tctcgctgct gcgcgtcggt ctcgcggtcg gggtgcagcg cgctggccag
     1321 cttgcggtag atctcgcgca ccgactgtgt ggcctgctgc gcctcggcct cgcgcttgtg
     1381 ttgtgccgcg ctcttgcgcc ggcgcccggc cttcgcgctg cgctgggcct cctcggccgc
     1441 cacctgctcc tgcaggcctt gctgcagccg cgcgaacagc tcggcgtcgc tgtcgatgcc
     1501 ttcgtcgtcg cccaggtcca gtccggtcat ggcttcggcc atgtccttca tggccagcat
     1561 cctgtcgcgc tgctcggtgt cgaagtcgac ttccgagtgc ttgtcgaaca gcgccctgat
     1621 ctgctcgtcc tcgccgcgcc cggccagcag ttcgcccgcg gcctcgcaca gcacttcccg
     1681 cagcgtggcg cgctcggcct tggtccagct cgcctgctcc agcgcggcat ccagcgccag
     1741 cacccattgc cggtgcccgg cgagcaactc ggcctgcagc ggccgcagca ccttgccatg
     1801 cgcctgccgg tacgccgcga cgttgtcctg ccacgccgcc aacgtctgtc gcgcctgctc
     1861 gatctggcgg atcagcgtgt tgaaccgctt ctgcgatggg ctcaacgcag ggccggcttg
     1921 ggtggaggag atttgcaggg cgtcagtgcg cgacacgggg ccagctccag cttgaacagg
     1981 tggccggcat tgtcgttcat gcagtccgac cgccgcgtgc gctccgcaca caccaatccg
     2041 cgtcctcgtc gccggataga agcggttcta tgccgccgct aacacctcgc gcgagaggat
     2101 ctcgggcagc cgctgcgggg ccggggccaa ggggatctgg aacgccccgc catccagccc
     2161 gagcacggtg ccgtacagga agcggaaggc gcagccgtac tgattgacgc tggagcgcgc
     2221 caggcgtcgc tcgcgcacca gatgcagcag gtagcgctgg acctcctcgg ccgtgagcgc
     2281 ctcggggctg cgtccatagt gccgagccaa tcgcgccacg gcctcgatgt aggactcccg
     2341 tgtgcgcact gccgcccccc gcagctccag cgcgtcgagc atgcgttgtc gaagtggtgt
     2401 catggccatc tcctcaatcg atgcacgggc acatgcccgc atcgatcaag gtagccccat
     2461 caccagcgcc tccccgcgct caggcaacca ccgcgtcagc ggtttagttc aacaagtaat
     2521 agacggccga aatgtcgcaa ttgtctgcgg cattaaactg gcccgcccga atcttctccc
     2581 gtcacgacaa tgacttagta cgctgctcca ctgccggggt cgggaacctg tcatgttcca
     2641 tagtggagcc gggcatggaa gcggtgtcta tccctccgaa gcaggaagag ctcgcgctca
     2701 agccgcggct gctcgaccag gtgcgcgact cgctgcgccg acgccgctac agcctgcgca
     2761 cggagaaggt gtacgtgcac tggatccgcc gctacatcct gttccacggc aagcgccacc
     2821 cggccgagat gggcgcgcgc ccgaggtgac cgccttcctc agcgatctgg cgcgccggca
     2881 ccgggtggcc gcctccacgc agaaccaagc gttgtcggcg ctgctgtatc tgtacggcca
     2941 gatcctggag atgaagctgc cctggctgga cggcgtcgag cgggccaagc gcccggcgcg
     3001 cttgcccacg gtgctgacgg tggccgaggt gcagcaggtg ttggcgaacc tggagggaac
     3061 gaagcacctg atggtctcgc tgctctacgg tgccggtctg cgcctgatgg agtgcctggg
     3121 cctgcgggtg aaggacatcg acttcgagcg cgggcagctc atcgtgcgcg acggcaaggg
     3181 cgggcgggac cgagtgacgg tgctgccgca gtgcgtcgct gacgagctgc gccggcatct
     3241 tgagcgggtg cggctgctac taaggtgcgc ccgagatatg cacgccggaa gccatgatcc
     3301 cctggttcga tggacggcca gccccgacaa tcatgcaaac atgaaatgcg aggagcgtct
     3361 ctttgaacca ggccgagcac cccaaccagt tcgcactact cacgcagcgc cgcttcgccc
     3421 ccttcttctg gacccagttc ctcggcgcgg cgaacgacaa cctgttcaag ttcgccttca
     3481 cggtgctggt gacctaccag ctgtcgatca gctggctgcc gcccgagctg gcgggcctga
     3541 cgatcggcgc gctcttcatc ctgcccttcc tgctgttctc ggccaccagc ggccagctgg
     3601 ccgacaagct ggagaagacg cggctgatcc gcttcgtcaa gtggctggag atcgccatca
     3661 tgtcgctcgc ggcctggggc ttcgtcactg cgcaggtgag cgcgctgctg gcctgcgtgt
     3721 tcctgatggg cctgcattcg acgatcttcg gcccggtcaa gtacgcctac ctgccccagc
     3781 acctgaacga acgcgagctc acgggcggca ac
//
LOCUS       DASUCY010000471         2171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_685121, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000471 DASUCY010000000
VERSION     DASUCY010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685121"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="VFV25_09400"
     CDS             complement(<1..704)
                     /locus_tag="VFV25_09400"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HEY1393582.1"
                     /translation="MTHPKELPLASLQFYATAPYPCSYLPGRLARSQVATPSHLIHAD
                     AYSGLVASGFRRSGMFTYRPYCDGCKACVPLRVPVESFESSRSQRRAWRAHRGLQARV
                     LRLCFVPEHYQLYLRYQAGRHAGGGMDHDSIDQYTQFLLQSRVNSRLVEFRESAPEGG
                     LGELKMVSILDILNDGLSAVYTFYEPEAGTSYGTYSIMWQLEQARVLKLPYVYLGYWI
                     AQSGKMAYKSQFRPHEV"
     gene            complement(701..1495)
                     /gene="aat"
                     /locus_tag="VFV25_09405"
     CDS             complement(701..1495)
                     /gene="aat"
                     /locus_tag="VFV25_09405"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEY1393583.1"
                     /translation="MIRWLDNPDTPFPDTRMALPAGSQAPGLLAAGGELSPERLAGAY
                     EHGIFPWYGQGQPVLWWSPDPRMVLPVREFRLSRSLRKALQRFIATPGCELAIDRDFE
                     RVMRCCAHAARPGQDGTWILPEMVRAYAAWHRLGDRRSSVHSFETWIDGELVGGLYGV
                     SIGRMFFGESMFSLRTDASKIALAALVAFALARGIPIIDCQQNTAHLASLGAREMARV
                     DFEQQLASAVQAPPIADWTYDPQLWAGLDARLKPYQAPPPGKAVHQ"
     gene            complement(1772..>2171)
                     /gene="phaP"
                     /locus_tag="VFV25_09410"
     CDS             complement(1772..>2171)
                     /gene="phaP"
                     /locus_tag="VFV25_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428554.1"
                     /note="Members of this family are phasins (small proteins
                     associated with inclusions such as PHA granules). Note
                     that several different families of phasins have been named
                     PhaP despite very little sequence similarity to each
                     other; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR01841 family phasin"
                     /protein_id="HEY1393584.1"
                     /translation="AFSVKDAQELLALQAGLLQPAAEKAAAYSRHVYDIASATNAEVS
                     KVAEETVADAQQKFLSLVDTAVKNAPAGTESAVALVKSAVNAANNAFESVQKAAKQAA
                     DVAEANMQALNATAAKAASQATPKSRRAAA"
BASE COUNT          358 a          675 c          771 g          367 t
ORIGIN      
        1 acctcgtgcg ggcggaactg ggacttgtag gccatcttgc cgctctgcgc gatccagtag
       61 cccagataga catagggcag cttcagcacg cgggcctgct ccagctgcca catgatgctg
      121 taggtgccgt agctcgtgcc cgcttcgggc tcgtagaagg tgtagaccgc cgacagcccg
      181 tcgttcagga tgtcgaggat ggacaccatc ttcagctcgc cgaggccgcc ttcgggcgcg
      241 ctttcgcgga actcgaccag cctcgaattg acgcggcttt gcagcaggaa ctgggtgtac
      301 tggtcgatgg agtcgtggtc catgccgccg ccggcatggc gccccgcctg atagcgcagg
      361 tagagctggt agtgctcggg cacgaagcac agcctcagca cgcgcgcctg caggccgcgg
      421 tgcgctcgcc aggcgcggcg ctgactgcgg ctcgattcga acgactcgac cggcacgcgc
      481 agcggcacgc aggccttgca gccgtcgcaa tagggccggt aggtgaacat gccgctgcgc
      541 cggaagccgc tggcgaccag gccggaatag gcatcggcat ggatcagatg gctgggcgtg
      601 gcgacctggg agcgggccag gcgccccggc agatagctgc aagggtaggg agccgtcgca
      661 tagaactgca acgacgcgag cgggagctct ttcggatgcg tcactggtgt acggccttgc
      721 ctggcggggg tgcttgatat ggtttcaacc tggcatccag ccccgcccac agttgaggat
      781 cataggtcca atcggcgatc ggcggcgctt gcacggcgct tgcgagctgt tgctcgaaat
      841 ccacgcgggc catctcgcgc gcgcccagcg aggcgaggtg ggcggtgttc tgctggcagt
      901 cgatgatcgg tatgccgcgc gccagcgcga aggccacgag ggccgccagc gcgatcttgg
      961 acgcatcggt gcgcagcgag aacatcgatt cgccgaagaa catgcggccg atgctcacgc
     1021 cgtaaagacc gcccaccagt tcgccgtcga tccaggtctc gaaggaatgc accgaggatc
     1081 gccgatcgcc gagacggtgc caggccgcgt aggcgcgaac catctcgggc aggatccagg
     1141 tgccgtcctg gcccggtcgc gcggcatggg cgcagcagcg catcacgcgc tcgaagtcgc
     1201 ggtcgatggc gagctcgcag cccggcgtcg ctataaagcg ctgcagggcc ttgcgcagcg
     1261 agcggctgag gcggaactcg cgcaccggca gcaccatacg cgggtccggg ctccaccaca
     1321 acaccggctg gccttgccca taccaaggga agatgccgtg ctcataagca ccggccaggc
     1381 gctcagggct cagctcgccg cctgcggcca gcaatccggg cgcctggctg ccggccggca
     1441 gggccatgcg ggtgtccgga aacggggtat ctgggttgtc cagccagcgg atcacggtgt
     1501 cggggtttgc gctatgcctg cctcgatttt gacgtgccgc tgcggcactc agcgctgctt
     1561 ccgcggccgg aaggggccga aaagggctcg ggaaaaagaa agggccctgc gttaccgcgg
     1621 ggcccgtaag gtaccttgaa aggagggttt cgacaggtac cgaggagaca accttccaga
     1681 gagaccgccg gcaaggccag cggcttatgt cagataagtc tcaagcgggg aagttcaacc
     1741 ctcgttcact tctttacgtc tgttcaagaa ctcaggctgc ggcgcggcgg ctcttgggcg
     1801 tggcctggga ggcggccttg gcggcggtgg cgttcagggc ctgcatgttg gcttcggcca
     1861 cgtcggcagc ctgcttggcg gccttttgca cgctttcgaa ggcgttgttg gcggcgttga
     1921 cggccgactt gaccagggcc acggcgctct cggtgccggc tggggcgttc ttgacggcag
     1981 tgtcgaccag cgacaggaac ttctgctggg cgtcggccac ggtttcctcg gcgaccttgc
     2041 tcacttcggc gttggtggcc gaagcgatgt cgtacacatg gcggctgtag gcggcagcct
     2101 tttcggcggc gggctgcagc aggccggctt gcagggccag cagctcctgg gcgtccttga
     2161 ccgagaaggc g
//
LOCUS       DASUCY010000472         3264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_235288, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000472 DASUCY010000000
VERSION     DASUCY010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235288"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1201)
                     /gene="ntrC"
                     /locus_tag="VFV25_09415"
     CDS             complement(<1..1201)
                     /gene="ntrC"
                     /locus_tag="VFV25_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HEY1393585.1"
                     /translation="MKSIWIVDDDQSIRFVLEKALAREDLPVRSFSNARDVLAALDQQ
                     DEPQVVVSDIRMPGGSGLDLLGKLKERLPGLPVIVMTAYSDLDSAVSSFQSGAFEYLP
                     KPFDITKAVELIRRAVDESVREEVRDSAQAPMPEMLGQAPAMQDVFRAIGRLSQSNVT
                     VLITGESGSGKELVARALHKHSPRGDLGNKGPFVAINTAAIPKDLLESELFGHERGAF
                     TGAQTMRRGRFEQADGGTLFLDEIGDMPFDLQTRLLRVLSDGQFYRVGGHHPLKSNVR
                     VIAATHQNLEERVRAGTFREDLFHRLNVIRLRLPALRERREDIPMLARHFLQQSAKQL
                     GVEPKRISDAALARLTAFGFPGNVRQLENICHWLTVMAPGQRVDVPDLPPEVREKPGA
                     PGAPADID"
     gene            complement(1253..2341)
                     /gene="glnL"
                     /locus_tag="VFV25_09420"
     CDS             complement(1253..2341)
                     /gene="glnL"
                     /locus_tag="VFV25_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003077515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HEY1393586.1"
                     /translation="MTAAPAEQANLRRYEALDHLATMVAVLRPDGDCLFANAAFEQVL
                     GLSRRAVLRGSLFDWFTEPELLRETMAAVARNEFSTSRFESQLKRHSGAHGEPLPVHV
                     IVTQMDRGALMLVEMVEIEQQTRQDREERALDQAQANKELIRNLAHEIKNPLGGIRGA
                     AQLLQMEVESRELTEYTQVIIHEADRLQALVDRLLAPHRKPHVVGDVNIHEICERVRA
                     LILVEHPRGLKVLRDYDTSLPEFRGDREQLIQAVLNIAQNAAQALGERIAAGDACIML
                     RSRAARQVTLGKQRYRLALELHIEDNGPGVPEAIRDRIFYPLVSGREGGSGLGLTLAQ
                     TFVQQHQGTIECESEPGRTIFKILLPLP"
     gene            complement(2338..2823)
                     /locus_tag="VFV25_09425"
     CDS             complement(2338..2823)
                     /locus_tag="VFV25_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393587.1"
                     /translation="MKLPSISSLSLLLAGSLFAGAAWADTGVYRCPGNNFTNSITAKE
                     AQAKGCKPVEGGNVTVVASPKRTAPAASPRASDSKVDPADQKARDSDSRRILEDELRK
                     EEEQLAGLQKEYNNGEPERLGNERNYQKYLDRTANLKAAIERTQSDIDALRRELAKYP
                     Q"
     gene            complement(2989..>3264)
                     /gene="glnA"
                     /locus_tag="VFV25_09430"
     CDS             complement(2989..>3264)
                     /gene="glnA"
                     /locus_tag="VFV25_09430"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549454.1"
                     /note="forms a homododecamer; forms glutamine from ammonia
                     and glutamate with the conversion of ATP to ADP and
                     phosphate; also functions in the assimilation of ammonia;
                     highly regulated protein controlled by the
                     addition/removal of adenylyl groups by adenylyltransferase
                     from specific tyrosine residues; addition of adenylyl
                     groups results in inactivation of the enzyme; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEY1393588.1"
                     /translation="GLDGVENKIHPGEAATKDLYHLAPEEDAKIPTVCSSLDQALDYL
                     DKGRAFLTKGGVFTDAFIDAYIELKMQEVTRFRMTTHPVEFDMYYAL"
BASE COUNT          477 a         1075 c         1126 g          586 t
ORIGIN      
        1 agtcgatgtc ggccggcgcg cccggggcgc ctggcttttc gcgcacctcg ggcggcaggt
       61 cgggcacgtc gacgcgctgc ccgggcgcca tcacggtgag ccagtggcag atgttctcga
      121 gctggcgcac gttgccgggg aagccgaacg cggtcaggcg cgccagcgcg gcgtcggaga
      181 tgcgcttggg ctccacgccc agctgcttgg ccgactgctg caggaagtgg cgcgccagca
      241 tcgggatgtc ctcgcgccgc tcgcgcagcg ccggcaggcg cagccggatg acgttgaggc
      301 ggtggaacag atcctcgcgg aaggtgccgg cgcgcacgcg ctcctcgagg ttctggtggg
      361 tggccgcaat gacccgcaca ttgctcttca gcgggtgatg gccgccgacg cgatagaact
      421 gcccgtccga cagcacgcgc agcaggcgcg tctgcaggtc gaagggcatg tcgccgatct
      481 cgtcgaggaa gagcgtgccg ccgtcggcct gctcgaagcg cccgcggcgc atagtctgcg
      541 cgccggtgaa ggcgccgcgc tcgtggccga agagctcgct ttccagcaag tccttgggaa
      601 tcgccgcggt gttgatcgcg acgaaggggc ccttgttgcc gagatcgccg cgcgggctgt
      661 gcttgtgcag cgcgcgcgcc accagctcct tgcccgagcc gctctcgccg gtgatcagca
      721 ccgtgacatt gctctggctg agccgcccga tggcgcggaa cacgtcctgc atggccggcg
      781 cctggcccag catctcgggc atcggggcct gggcgctgtc gcgcacctcc tcgcgcacgc
      841 tctcgtcgac ggcgcggcgg atcagctcca ccgccttggt gatgtcgaaa ggcttgggca
      901 ggtattcgaa ggcgccgctc tggaacgagg aaacggcgct gtccaggtcg gaataggcag
      961 tcatcacgat gaccggcagc cctggcaggc gctccttgag cttgcccaac aggtccaggc
     1021 ccgagccccc cggcatgcgg atgtcgctca ccaccacctg tggttcgtcc tgctggtcga
     1081 gagccgccag cacgtcgcgc gcattgctga agcttctgac cgggaggtct tcgcgcgcta
     1141 gcgccttctc caatacgaag cgtatggatt ggtcgtcgtc aacgatccag atggatttca
     1201 tgcctgccct gcgcccctcg gctctgctgt aggccgccga cccgactcgt gatcagggca
     1261 gcggcagcaa tatcttgaaa atggtacgcc ccggctcgct ctcgcattcg atcgtgccct
     1321 ggtgttgctg cacgaaagtc tgcgccagcg tgagccccag cccagagccg ccctcgcgtc
     1381 ccgataccag cgggtagaag atgcgatcgc ggatggcctc gggcacgccc ggtccgttgt
     1441 cctcgatatg caattccagt gccagacggt agcgctgctt gcccaacgtg acctggcgcg
     1501 ccgcgcggct gcgcagcatg atgcaggcgt cgccggccgc gatgcgctcg cccagggcct
     1561 gggccgcgtt ctgcgcgatg ttgagcaccg cctggatcag ctgctcgcgg tcgccgcgga
     1621 actcgggcag cgaggtgtcg tagtcgcgca gcaccttcag gccgcgcgga tgttccacca
     1681 ggatcagcgc gcgcacccgc tcgcagatct cgtggatgtt gacgtcgccc accacatgag
     1741 gcttgcggtg cggggccaga agccggtcga ccagggcctg cagtcgatcg gcctcgtgga
     1801 tgatgacctg ggtgtattcg gtcagctcgc gggactcgac ctccatctgc agcagctggg
     1861 ccgcgccgcg tatgccgccc agcgggttct tgatctcgtg cgccaggttg cggatcagct
     1921 ccttgttggc ctgggcctgg tccagggcgc gctcctcgcg gtcctggcgc gtctgctgct
     1981 cgatctcgac catctcgacc agcatcagcg cgccgcggtc catctgcgtg acgatgacgt
     2041 gcaccggcag cggctcgcca tgggcgcctg aatggcgctt gagctggctc tcgaagcggc
     2101 tggtggagaa ctcgttgcgc gccaccgccg ccatcgtttc gcgcagcagc tcgggctcgg
     2161 tgaaccagtc gaacagcgag ccgcgcagca cggcacggcg cgacaggccc agcacctgct
     2221 cgaaggcggc gttggcgaac aggcagtccc cgtccgggcg cagcacggcc accatggtgg
     2281 ccaggtgatc cagcgcttcg tagcgccgga ggttcgcctg ctcagccggt gcggcggtca
     2341 ctgcggatat ttggcgagtt cgcggcgcag tgcgtcgata tccgattgcg tgcgctcgat
     2401 cgcggccttc aggttggcag tgcgatccag atacttctgg tagttgcgct cgttgccgag
     2461 gcgctcgggc tcgccgttgt tgtattcctt ctgcagcccg gcgagctgct cctcttcctt
     2521 gcgcagctcg tcttcgagga tgcggcgcga atcgctgtcg cgcgccttct ggtcggcggg
     2581 gtcgaccttg ctgtcggagg cgcggggcga tgccgccggg gcggtccgct tgggcgaggc
     2641 cacgacggtc acgttgccgc cttcgaccgg cttgcaaccc ttggcctggg cctccttggc
     2701 cgtgatggag ttggtgaagt tgttgccggg gcagcggtaa acgccggtgt cggcccaggc
     2761 ggcgcctgca aacaggctgc cggcgagcag caggctcagt gaagatatag acggcagttt
     2821 catcgaggga actcctgccc cttccaacgt gcgtatacag cggcgcgctg acgaggcgcc
     2881 ggcagtatga ctgaaacgtc agcctggggc aaaaccgcag cgtcagcgtc agccagaaca
     2941 aaaaagggac ggctcgcgcc gtcccctttc agcctcggat cccagggatc acagggcgta
     3001 gtacatgtcg aactcgaccg gatgcgtggt catgcggaag cgcgtgactt cctgcatctt
     3061 gagctcgatg taggcgtcga tgaaggcatc ggtgaagacg ccgcccttgg tcaggaaagc
     3121 gcggcccttg tccaggtagt cgagcgcctg gtccaggctc gagcagacgg tcgggatctt
     3181 cgcgtcttct tccggcgcca ggtgatacag gtccttggtg gcggcttcgc ccggatggat
     3241 cttgttctcc acgccgtcca ggcc
//
LOCUS       DASUCY010000473         2846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1038784, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000473 DASUCY010000000
VERSION     DASUCY010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038784"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..1381
                     /gene="hmgA"
                     /locus_tag="VFV25_09435"
                     /pseudo
     CDS             77..1381
                     /gene="hmgA"
                     /locus_tag="VFV25_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227576.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
     gene            1378..2688
                     /gene="fahA"
                     /locus_tag="VFV25_09440"
                     /pseudo
     CDS             1378..2688
                     /gene="fahA"
                     /locus_tag="VFV25_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347071.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
BASE COUNT          464 a         1007 c          923 g          452 t
ORIGIN      
        1 gccaggaggc gctcctggag gcactggaga cggcacggcg cagcacttta agaagccccg
       61 cacaggagac cagaggatga ccgccaccga cttgctgtac caatccggct tcggcaacga
      121 gttcgccacc gaggcgctga cgggcgcgct gcccgtgggc cgcaattcgc cgcagcgctg
      181 cccctaaggg ctttacgccg agcagctcac cggctccgcc ttcaccgcgc cgcgcggcgc
      241 caatcgccgc tcatggctgt accgcatccg cccggcggcg acgcaccagc ccttcgagcg
      301 catcaacaac ggccgcatcg tcagcgattt ctcggcgctt gtcgcaccgc ccgatcagct
      361 gcgctggagc ccgatgccgc tgccgacatc gccgaccgat tttgtcgacg gcctggtcac
      421 gatggctggc catggcgacc cgcatgtcca ggccggcgcg gcctgccata tctacgctgc
      481 gaaccgctcg atgcagcggc gcgccttcta cagcgccgac ggcgagctgc tgatcgtgcc
      541 gcagcagggc cggctgcgct tcgtcaccga gctgggcacg ctggaggccg agccgcagga
      601 ggtcgtcgtc attccgcgcg gcgtgcgctt tcgtgtcgag ctgctggacg cggcggcgcg
      661 cggctatgtt tgcgagaact tcggggcgcc tttcaagctg ccagacctgg ggcccatagg
      721 ctccaacggg ctggccaacc cgcgcgactt cttgacgcca gtcgccgctt acgaagacat
      781 cgacggcccg cacgagctgg tggccaagtt catggggcag ctgtggcgcg ccgagatgga
      841 tcactcgccg atcgacgtgg tggcctggca tggcaactat gcgccctaca agtacgacct
      901 gcggcgcttc aacgccgtcg gctcgatcag ctacgaccac ccggaccctt cgatcttcct
      961 cgtgctgcat tcggcgtccg acacgccggg cgtcagcgcg atcgacttcg tgatcttcgc
     1021 gccgcgcatc cttgcgatgc gtgacacctt ccgcccgccc tggttccatc gcaacgtcgc
     1081 cagcgagttc atgggcctcg tcaccggtgt ttatgacgcc aaggccgagg gctttctgcc
     1141 gggcggcgcg agcctgcaca acagcatgac cgggcatggc ccggacgccg agaccttcga
     1201 gcgcgccagt cacgcggatt tgtccaagcc ggacgtgatc gagaacacca tggccttcat
     1261 gttcgaagcg cgtcacgtgt ggcggcccac gcccttcgcg ctggagagcg ccgaacgcca
     1321 gcgcgagtac tggcgctgct ggcagggtct gaagaagcat ttcaacccgc gacagccatg
     1381 aacaaggatg ccacccacga cccagggctg cgcagctggg tcgagtcggc caatgcagcg
     1441 gacaccgact tcccgatcca gaacctgccc tatggccgct tccgccgcgc gggcagcgat
     1501 gaagcctggc gcatcggcgt cgcgatcggt gaccagatcc tggacctgcg gctggcgctg
     1561 gagcagtgcc cctggagcga cgagatcgcc gagctgctgc cgccgttggc gcagggcgat
     1621 ctgaaccgct tcatggccca gggctcagct gcacgcaaga cgatgcgcgc agccctctcc
     1681 gaggccttgc aggccaatag cctgcagggc ccattccttg agctgtgcct gctgccgcag
     1741 gctgaggccg agatggcctt gccctgcgag atcggcgact acaccgactt ctacaccggc
     1801 atccaccatg cgaccacagt gggcaagctg ttccgccccg acaaccccct gatgcccaac
     1861 tacaagtggg tgccgatcgg ctatcacggg cgcgcctcgt ccatcgtcgt cagcgggcag
     1921 gcgatacgcc gcccgcgtgg gcagaccaag ggcagcgccg acatgccgcg gttcggcccc
     1981 agccagaagc tggactacga gctggagctc ggcttcctga tcgggccggg caacgtcctg
     2041 ggcgagccca tcgcgatagc ggacgcggag gagcatctct tcggcgtgac cctgttcaac
     2101 gactggtcgg cgcgcgatat ccagccctgg gaataccagc cgctcgggcc tttcctgtcc
     2161 aagaacttcg ccagcagcgt ctcgccctgg gtcgtcacga tggaagcgct ggcgcccttc
     2221 cgttctcctt tcgtgcgccc cgagggcaat ccgtagcccc tgccctatct cgactcggcg
     2281 agcaaccgcg agcagggcgc cctcgagatc cagctcgagg cctggctgca gacggcgcag
     2341 atgcgcgcgg caggccagcc gggcgtgcga ctcgcgggcg ccaactctgc agcagcggcc
     2401 tattggacgc cggcccagct ggtggcccac cacacggcag gcggctgcaa tctgcggccg
     2461 ggtgacctgc tcggctcggg cacgctgtct ggcccgagcc ccgagcaggc cggttcactg
     2521 ctagaactga cgctgggcgg caagcagcca ctgcatctgc ccaacggcga gacgcgcagc
     2581 ttcctggccg acggcgacac gctgagcttg cgcgcctggt gcgagcgcga tggggcaagg
     2641 cgcatcggct tcggcgtgtg cacgggcacg gtgctcgccg cgccgtagca agggcggaat
     2701 gaagaaagcg cctcagtcga ggcgcttttc tcttgggggt aacgagtgcc tcaggcgcgc
     2761 gccgcacgca gcttgagcgc gaactcctgt aacgccgcaa tgccgctttc ctcggccttc
     2821 ttgcaccaag cctgcagatc ggcaac
//
LOCUS       DASUCY010000474         3421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_621264, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000474 DASUCY010000000
VERSION     DASUCY010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621264"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /locus_tag="VFV25_09445"
     CDS             <1..983
                     /locus_tag="VFV25_09445"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEY1393589.1"
                     /translation="TVPGPGEAAHDTWTGDSWKTGGAPTWTVGAYDLEQDLIIWGTGN
                     PSPEFQAKLRPGDNLYSNSVIAVEAKTGRLRWHYQFTPADEHDWDSCQQPILADIVWQ
                     GRKRAVVLWANRNAFFYALDRKSGEFLFAKPFVKQSWNEGFDAKGRPRTSADAKPSAA
                     GALVWPAIMAATNWWPPSYDAARQLVFVPCSEAAGIYVQRDDVEYKKGERFEGSAASV
                     YAPNLPTAAYVKAVDAQTGEVRWQTALNSNSENFVWTVGGILSTQGGVVFSGYRDYFQ
                     AFDADDGKKLWRINLGARVRGSPVSFLMDGRQYIAVAAGHSIFVFMLPQA"
     gene            1128..2396
                     /locus_tag="VFV25_09450"
     CDS             1128..2396
                     /locus_tag="VFV25_09450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEY1393590.1"
                     /translation="MGTYLLTLPWGLGTVGGVTQVVTGLYDAIKSDARLTPRVLVASW
                     EDQEPVEQVDAEGRLVVRCRIRSPGGRGPAIYGLLRYLAALPRELARIRALVQRYSVE
                     VVNCHYVGSSEFTWVLAKSLGIYRGKVFLSLHGLDIRSLAAARGLRRWPWAWALRHAD
                     AVVACSEGLAAETIAAFRLPKTQVVTIHNGVDAAQLARIASDAPDMPPTEGPSLLNLG
                     TFEHKKGHDLLLKAFCKVVDRHPTARLTIMGRQADSMESTLRLVAELGLADHTSIRTD
                     VPHEQALRALKHADVFVLSSRNEAFSVALLEAGALGKPVVATDVCGVPELIQDGKTGL
                     RVPPEDVDALAGGILRLLDDKEAAAEFGLRLRELVQDKFTLEANCRSYLRLAGCRPSD
                     PSSAAEDKLGEAVPGSSAAGQPRARAFPQR"
     gene            complement(2393..>3421)
                     /locus_tag="VFV25_09455"
     CDS             complement(2393..>3421)
                     /locus_tag="VFV25_09455"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011353701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEY1393591.1"
                     /translation="RAVRALLAPALLLEELPVTQTVEDLVEHKRQAIADVLHGRDDRL
                     VAVVGPCSIHDHDEALEYARRLKRLADGLAGELLVVMRVYFEKPRTTVGWKGYINDPR
                     LDGSFRINEGLQRARRLLLDIATLGLPAGTEFLDLLSPQYLVDLVAWGAIGARTTESQ
                     SHRQLASGLSCPIGFKNGTDGGVQVAADAMVAAAAPHSFIGMTKMGVAAIFETRGNDD
                     CHLILRGGKTPNYDAAHVEAACAALRKAGLREQVMVDCSHANSAKQHRRQIDVARDVA
                     AQIGAGEQRITGVMIESHLHEGRQDLRPGQPLLPGVSITDACLSWDDTEPLLRELAAA
                     VSKRRSTS"
BASE COUNT          556 a         1179 c         1133 g          553 t
ORIGIN      
        1 ataccgtccc cgggccgggc gaggcggcgc atgacacatg gaccggcgac agctggaaga
       61 cgggcggcgc tcccacctgg accgtaggcg cctacgatct cgagcaggac ctgatcatct
      121 ggggcaccgg caacccttcg ccggagttcc aggcgaagct gcggcccggc gacaacttgt
      181 acagcaacag cgtgatcgca gtcgaggcca agaccggccg cctgaggtgg cactatcagt
      241 tcacacctgc ggacgagcat gactgggatt cgtgccagca gcccatcctg gcggacatcg
      301 tctggcaggg gcgcaaacgc gcggtggtgc tgtgggccaa caggaatgcc ttcttctatg
      361 ccctggaccg aaagagcggg gagttcctgt ttgccaagcc tttcgtgaag cagagctgga
      421 acgaaggctt cgacgccaaa gggcgcccgc ggacgtccgc cgacgccaag cccagcgccg
      481 cgggcgcgct ggtctggccg gccatcatgg ctgcgaccaa ctggtggccg ccgtcctacg
      541 atgccgcgcg ccagctggtg ttcgtgccct gctccgaggc cgccgggatc tacgtccaga
      601 gagacgacgt ggagtacaag aagggcgaac gcttcgaagg aagcgctgcc tcggtctatg
      661 cgcccaacct tcccaccgcc gcctatgtca aggccgtcga tgcccaaaca ggcgaagtgc
      721 ggtggcagac ggccctcaac tcgaacagcg agaacttcgt ctggaccgtc ggcggcattc
      781 tctcgacgca gggcggggtc gtcttctcgg gctaccggga ctatttccag gccttcgatg
      841 cagacgacgg gaagaagctc tggcgcatca atctcggggc tcgcgtgcgt ggctctcccg
      901 tctctttcct gatggatggt cggcaataca tcgccgtcgc agccgggcat tcgatcttcg
      961 tcttcatgtt gccgcaggcc tagggacgga agccaagctt gcaggcgcgt catcttgaaa
     1021 cttcgctgac aggcatgtcc acatcgcact gcccgcctta cagttctcgt gatggccaaa
     1081 gtccgagcta cccgcgcggc ccggcggcgc tgccagccga ccgcatcatg ggaacctacc
     1141 ttctgacgct gccttggggt ctcggcaccg taggcggcgt gacccaggtt gtcaccggtc
     1201 tctacgacgc gatcaagagc gacgctcgcc tcacaccgcg cgtcctggtg gcctcctggg
     1261 aggatcagga gcccgtggag caggtcgatg ccgaaggaag gctcgtggtt cgctgccgca
     1321 tacgcagccc cggcgggcgg gggcctgcga tctacggcct cctgcgctat ctcgctgcct
     1381 tgccccggga gctggcccgg atccgcgcgc tggtgcagcg ctactcggtc gaggtggtca
     1441 actgccacta cgtcggctcc tccgaattca cctgggtgct cgccaaatcg ctagggatct
     1501 atcggggcaa ggtcttcctc tcgctgcatg gactcgacat ccgcagcctc gcggccgcgc
     1561 ggggactcag gcgatggccg tgggcctggg cgctacggca cgcggatgcc gtcgtggcct
     1621 gctcggaagg cctcgccgcc gaaaccatcg cggccttccg gctccccaag acccaggtgg
     1681 tcacgatcca caacggcgtg gacgccgcgc agctggcgcg catcgcatcc gatgcgcccg
     1741 acatgccccc gaccgagggc ccgagcctgc tgaacctcgg gaccttcgag cacaagaagg
     1801 gtcacgacct tctgctgaag gccttctgca aggtcgtcga ccgccatccc accgcgcggc
     1861 tgaccatcat gggacggcag gccgacagca tggagtcgac gctgcggctg gtcgcggaac
     1921 taggtctggc ggatcacacg agcatccgca ccgacgtgcc gcacgaacag gcgctacgcg
     1981 ccctcaagca tgccgatgtg ttcgtgctgt cctcgcgcaa cgaggccttc tcagtggcac
     2041 tgctggaggc cggtgcgctc ggcaaacccg tcgtcgccac cgatgtctgc ggcgtgcccg
     2101 agctgataca ggacggcaag accgggctgc gcgtgccgcc cgaggacgtc gatgccctgg
     2161 ccggcggcat cctgcgcctg ctcgacgaca aggaagccgc cgccgaattc ggccttcgcc
     2221 tgcgcgaact ggtgcaagac aagttcacgc tggaggcgaa ctgccggagc tatctccggc
     2281 tcgcgggttg tcgccctagc gacccttcat ccgctgccga ggacaagctt ggcgaagcgg
     2341 tacccggcag ctctgccgcc ggccagccgc gggcgcgagc ctttccgcag cgttaactgg
     2401 tcgagcggcg cttgctcaca gcagcagcca gctcgcgcag caaaggttcg gtgtcgtccc
     2461 agctcaggca ggcatcggtg atcgagacgc cgggcagcag cggctggccg gggcgcaggt
     2521 cctggcggcc ttcgtgcaga tggctctcta tcatcacgcc ggtaatgcgc tgctcgccgg
     2581 cgccgatctg ggccgcaaca tcgcgggcga cgtcgatctg ccggcggtgc tgcttggccg
     2641 aattggcatg cgagcagtcg accatcacct gctcgcgcag gccagccttt cgcagcgccg
     2701 cacaggccgc ttcgacatgg gcggcgtcgt agttcggcgt cttgccgccg cgcaggatca
     2761 ggtggcagtc gtcattgccg cgcgtctcga agatcgccgc cacgcccatc ttggtcatgc
     2821 cgatgaagga atgcggtgcg gccgcggcga ccatggcatc ggccgccacc tgcacgccgc
     2881 cgtcggtgcc gttcttgaag ccgatcgggc aggacaagcc ggacgcaagc tggcggtggc
     2941 tctggctctc cgtcgtgcgc gccccgatgg cgccccaagc caccagatcg accagatatt
     3001 gcggcgacag caggtcgagg aactcggtgc cagccggcag gcccagcgtc gcgatgtcca
     3061 gcagcaggcg gcgcgcccgc tgcaggccct cgttgatgcg gaagctgccg tccaggcgcg
     3121 gatcgttgat gtagcccttc cagcccaccg tggtgcgcgg cttctcgaag tagacgcgca
     3181 tcacgaccag cagctcgccg gccagcccgt cggccaggcg cttcaggcgc cgcgcgtatt
     3241 ccagcgcctc gtcgtgatca tggatcgagc agggcccgac gactgccacc agccggtcgt
     3301 cgcgcccatg cagcacgtcg gcgatggcct ggcgcttgtg ctcgaccagg tcctcgacgg
     3361 tctgggtgac gggcaactcc tccagcagca gggccggcgc cagcagcgcc cgcacggccc
     3421 g
//
LOCUS       DASUCY010000475         2099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1006989, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000475 DASUCY010000000
VERSION     DASUCY010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006989"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="VFV25_09460"
     CDS             complement(<1..917)
                     /locus_tag="VFV25_09460"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEY1393592.1"
                     /translation="MSDPIVIVSAARTPIAGLLGDFSALAAWELGAVAIKAAVERAGV
                     PGDAVNEVLFGNCLMAGQGQAPARQALRKAGLPDSAGAVTLSKMCGSGMRAMMFAHDM
                     LAAGTADVLVAGGMESMTNAPHLMFARKGIKYGASAVYDHMALDGLEDAYERGKAMGV
                     FAENCVAKYGFSREAQDAFAIASTERALKANSDGSFDWEMAPVAVAGKGGEVKIAKDE
                     QPFKAKLDKIPGLKPAFKKDGSITAATSSSISDGAAALVLMRESDAAKRGLKPLARIA
                     AHAVHAQAPEWFTTAPVGAIDKVLKKTGWA"
     gene            complement(945..>2099)
                     /gene="aceK"
                     /locus_tag="VFV25_09465"
     CDS             complement(945..>2099)
                     /gene="aceK"
                     /locus_tag="VFV25_09465"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HEY1393593.1"
                     /translation="NKGAYVVGKIINGFVELPFALPILHDRNGKFYIDAALFGEDDLQ
                     MLFSFARAYFMVDMEIPAAYVQFLRSLMPRKPRSEIYSALGLQKQGKNLFYRDFLYHL
                     RHSSDRFRIAPGIKGMVMLVFDLPSFPYVFKVIKDEFPPQKDTTREQIMGKYLLVKQH
                     DRVGRMADTLEYSNVAFPRHRFDEELIADLKRECASLIEEDRDMLVIRHVYIERRMIP
                     LNIYLQEATPAQIEHGVIEYGNAIKDMVAANIFPGDMLWKNFGVTRHGKIVFYDYDEI
                     EYLTDVNFRRVPQAHNEEEELSAEVWYRVGPKDVFPETFGPFLLGNAAVREVFLKHHA
                     DLLDAAFWQAHQERIRAGDVHDVFPYDTSRRFIRQRRPAIADAAPAALAS"
BASE COUNT          345 a          655 c          723 g          376 t
ORIGIN      
        1 gcccagccgg tcttcttcag taccttgtcg atggcgccca ccggcgcggt ggtgaaccat
       61 tcgggagcct gggcatgcac ggcatgggcg gcgatgcgcg ccaggggctt gaggccgcgc
      121 ttggcggcgt cggactcgcg catcagcacc agcgcagcgg cgccgtcgga gatgctggac
      181 gaggtggcgg cggtgatgga gccgtctttc ttgaacgccg gcttgaggcc ggggatcttg
      241 tcgagcttgg ccttgaaggg ctgctcgtcc ttggcgatct tcacctcgcc gcccttgccg
      301 gccaccgcta cgggcgccat ctcccagtcg aagctgccgt cgctgttggc cttgagggcg
      361 cgctcggtgg aagcgatcgc gaaggcgtcc tgggcctcgc ggctgaagcc gtacttggca
      421 acgcagttct cggcgaacac gcccatggcc ttgccgcgct cgtaggcgtc ttccaggccg
      481 tccagcgcca tgtggtcgta gaccgcactg gcgccgtact tgatgccctt gcgcgcaaac
      541 atgaggtgcg gcgcattggt catgctctcc atgccgccgg ctaccagcac gtcggccgtg
      601 ccagcggcca gcatgtcgtg cgcgaacatc atcgcgcgca tgccggagcc gcacatcttg
      661 gacagcgtca cggcgccggc cgaatcgggc aggcccgcct tgcgcaaggc ctggcgcgcc
      721 ggggcctggc cctggccggc catcaggcag ttgccgaaca gcacctcgtt cacggcatcg
      781 ccgggcactc cggcccgctc gacggcggcc ttgatcgcga cggcgcccag ctcccaggcc
      841 gcgagcgcag agaagtcacc cagcaggccg gcgatgggcg tgcgggcggc ggaaacgatg
      901 acgatggggt cggacatggt gatgatctcc ttggggcaaa tgaatcagct ggccagagcg
      961 gccggcgcgg catcggcgat ggccgggcgg cgctggcgta tgaagcggcg ggaggtgtcg
     1021 taaggaaaca cgtcgtgcac atcgcccgca cggatgcgct cctggtgagc ttgccagaaa
     1081 gcggcatcca ggaggtcggc atggtgtttg agaaagacct cgcgcaccgc tgcattgccg
     1141 agcaggaagg ggccgaaggt ctcggggaag acgtccttgg ggccgacgcg gtaccagacc
     1201 tcggcggaca gctcttcctc ctcgttgtgc gcctggggca cgcggcggaa attgacgtcg
     1261 gtgaggtatt cgatctcgtc gtagtcgtag aacacgatct tgccgtggcg ggtgacgccg
     1321 aagttcttcc acagcatgtc gccggggaag atgttggccg ccaccatgtc cttgatcgcg
     1381 ttgccgtact cgatcacgcc atgctcgatc tgcgccggcg tggcctcctg cagatagata
     1441 ttgagcggga tcatgcggcg ctcgatgtag acatgccgga tcaccagcat gtcccggtcc
     1501 tcctcgatca ggctcgcgca ctcgcgcttg agatcggcga tcagctcctc gtcgaagcgg
     1561 tggcgcggga aggccacgtt gctgtactcc agcgtgtcgg ccatgcggcc cacgcggtcg
     1621 tgctgcttga cgagcaggta cttgcccatg atctgctcgc gcgtggtgtc cttctgcggc
     1681 gggaattcgt ccttgatgac cttgaacaca taggggaagg acggcaggtc gaacaccagc
     1741 atcaccatgc ccttgatgcc gggcgcgatg cggaagcggt cgctggagtg gcgcaggtgg
     1801 tagaggaagt cgcgatagaa caaattcttg ccctgcttct gcagccccag cgcgctgtag
     1861 atctccgagc gcggcttgcg cggcatcagc gagcgcagga actgcacata ggcggccggg
     1921 atctccatgt cgaccatgaa ataggcacgc gcgaagctga acagcatctg caggtcgtcc
     1981 tcgccgaaca gcgcggcgtc gatgtagaac ttgccgtttc ggtcgtgcag gatcggcagc
     2041 gcgaacggca gttcgacgaa accattgatg atcttgccga ccacgtaggc gcccttgtt
//
LOCUS       DASUCY010000476         4419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1408440, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000476 DASUCY010000000
VERSION     DASUCY010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408440"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..179
                     /locus_tag="VFV25_09470"
     CDS             <1..179
                     /locus_tag="VFV25_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEY1393594.1"
                     /translation="AMAGLSEDQRMAVSLVLIEGLPYKEAAEVLDIPIGTLTSRLARA
                     REALQATLMGGAGR"
     gene            176..928
                     /locus_tag="VFV25_09475"
     CDS             176..928
                     /locus_tag="VFV25_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393595.1"
                     /translation="MTTERYSDETLMAYVDHELDAASTAALEAAMQTDAALAERVARQ
                     RALSGRLRHAFDAVLDEPVPARLLAAAQGVPAAPTKVVPLAQAREQRAARDQARRLGW
                     PQWSGMGLALAACLMLGLWLGRPGPEPWANEGGRLVAQGELARALSTQLASSQAQDAP
                     VRIGVSFAAKSGEYCRSFALAHGPAGLACKDSAGAWRVPVLAEAGENNAAYRQAASAT
                     PAAVLGAIDAQIAGEPLDAEAERAAAQRGWRR"
     gene            1124..3139
                     /locus_tag="VFV25_09480"
     CDS             1124..3139
                     /locus_tag="VFV25_09480"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HEY1393596.1"
                     /translation="MLCAALLAAGLLSDQQALASPPVSGLATSDFDAKVRAQDDLFRH
                     VNGEWLKKTEIPADRASVGAFEQIHETIQPQLRGLIEEAAAKGSSDAQAHKIGDLYAS
                     FMDEAALEKLGAAPVAAELAAIDAVSDRKQLAALMARAIRAGIGMPLDVSIHQDAKDS
                     TRYIADAAQSGLGLPDRDYYLQLDDARFKEVRAKYQDFIAKMLALGGAKDTEASAREV
                     LALETELARAQWTRVENRDPVKTYNRVELAALPKLAPGFDWDGFLKEAGLAGKIDYLV
                     VSQPSYVTGLAQLLESQPLSAWKAYAKLRVLNAYAPYLSKDFVDTRFAFVGTVLRGTP
                     ENLPRWKRGVTLVEGSLGEGLGKLYVAQYFPPESKARMEELVANLLTAYKQSIDRLDW
                     MSPATKKQAQIKLAKFTPKIGYPKRWIDYSALEIKRDDLAGNVRRAREFEVNRQLAKL
                     GRAIDRDEWLMTPQTVNAYYNPELNEIVFPASILQPPFFDAKADDAVNYGGIGAVIGH
                     EISHGFDDQGSQYDGDGNLRDWWTKQDRARFNAKTKLLVKQYGAFEPVPGYPVNGELT
                     LGENIADNAGLAIAYKAYKLSLHGKPAPVIDGMSGDERFFYGFAQVWRGKMRDAALIQ
                     QLKSDPHAPDEIRANGTVRNHPGFYSTFKLKPGDAMYLPPSGRVTLW"
     gene            3248..>4419
                     /locus_tag="VFV25_09485"
     CDS             3248..>4419
                     /locus_tag="VFV25_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HEY1393597.1"
                     /translation="MHPDVQIPARCEVLVIGAGPAGSACALTLARAGLDVVLADQHAF
                     PRDKVCGDGLIPDAHAALRRLGVLDEVMAVAQAARHVGCVGPRGGRIDVPGTLAVLPR
                     RELDHILCRAAVRAGAKLLPPARFEAPLERDGKVSGARLKCGDGTLEIAADWLILATG
                     AVPQAMIAAGLCTRRTPSGVALRGYVRNEAMATKLKALDVVWHRKLRPGYGWIFPCRD
                     GVFNIGVGLAHSHSKFSNGRRTMQDVNLRETFAAFCEFYEPARELMQGGTLLGELKGA
                     PLRCSLEGAKYSRPGLIATGEAVGSTYAFTGEGIGKAMETGMLAAEAVLQGRREQLDE
                     AAVRQHYESQLLALKPRFDLYEKANRVNEHPWLADLLIWRAKRSERILRRMSGVLE"
BASE COUNT          726 a         1540 c         1530 g          623 t
ORIGIN      
        1 aggcgatggc cggcttgagc gaggaccagc gcatggcggt gagcctggtg ctgatcgagg
       61 ggctgcccta caaggaagcg gccgaggtgc tggacatccc catcggcacg ctgaccagcc
      121 gcctcgcccg cgcccgcgag gccttgcagg cgacgttgat gggtggagca gggcgatgac
      181 gaccgagcgc tattccgacg aaacgctgat ggcctatgtg gaccatgagc tggacgcggc
      241 cagcaccgcg gcgctcgaag cggcgatgca gaccgacgcg gccctggccg agcgggtagc
      301 gcgccagcgc gccctgagcg gccggctgcg ccatgccttc gacgccgtgc tcgatgagcc
      361 ggtgccggcg cgcctgctgg ctgcggccca gggcgtcccg gctgcgccga ccaaggtcgt
      421 gccgctggcc caggcgcgcg agcagcgcgc ggcccgcgat caggcgcggc gcctgggctg
      481 gccgcagtgg agcggcatgg ggctggcgct ggcggcgtgt ttaatgctgg gcctgtggct
      541 gggccgcccc ggccccgagc cctgggccaa cgagggcggc cggctggtcg cgcaaggcga
      601 actggcgcgc gccctgtcca cccagctcgc aagtagccag gcccaggacg cgccggtgcg
      661 cataggcgtg agcttcgccg ccaagtctgg cgaatactgc cgcagcttcg cgctggcaca
      721 tggcccggcc gggctggcgt gcaaggacag tgcgggggcc tggcgcgtgc cagtgctggc
      781 cgaggccggc gaaaacaatg ccgcctaccg ccaggccgcg agcgccacgc cggccgcggt
      841 gctgggcgcc atcgatgcgc agatcgccgg tgaaccgctc gatgccgaag cggagcgcgc
      901 agcggcgcag cgcggttggc gccgctgaag caccggcccc gaggtcgtcc ggcccccgct
      961 ttccgccgtt cgtcgcatgc cgggggcggg ccgctcgccc gctcggctag agtcgcgcgc
     1021 agcgcggcgc cgaccggcgt cgccgccaca acactttggc ggccaccagc cgcatggagt
     1081 tcgacacgtg aatcttcgat ccggaaaatc tccgcgtgcc ctgatgctgt gcgccgccct
     1141 gctggccgcc ggcctgctga gcgatcagca ggccctggcc tcgccgcccg tctcgggcct
     1201 ggccacgtcc gatttcgacg ccaaggtacg ggcgcaggac gacctgttcc gccatgtcaa
     1261 cggcgaatgg ctcaagaaga cggagatccc ggccgatcgc gcctcggtgg gcgccttcga
     1321 gcagatccac gaaacgatcc aaccccagct gcgcggcctg atcgaggagg ccgcggccaa
     1381 gggcagcagc gatgcgcagg cgcacaagat cggcgacctc tacgccagct tcatggacga
     1441 ggccgcgctg gagaagctgg gcgccgcgcc agtggcggcc gagctggccg cgatcgacgc
     1501 ggtcagcgat cgcaagcagc tggccgcgct gatggcgcgc gcgatccgcg ccggcatcgg
     1561 catgccgctg gacgtctcca ttcaccagga tgccaaggat tcgacccgct acatcgccga
     1621 cgccgcgcaa agcggcctgg gcctgcccga ccgcgactac tacctgcagc tggacgacgc
     1681 ccgcttcaag gaagtgcgcg ccaagtacca ggacttcatc gccaagatgc tggccctggg
     1741 cggcgccaag gacaccgagg cctccgcgcg cgaggtgctc gcgctggaga ctgagctggc
     1801 gcgcgcgcaa tggacacggg tcgagaaccg cgacccggtc aagacataca accgcgtcga
     1861 gctggccgcc ctgcccaagc tcgcgcccgg cttcgactgg gacggcttcc tgaaggaggc
     1921 cgggctggcc ggcaagatcg actacctcgt cgtgagccag cccagctatg tgacggggct
     1981 ggcccagctg ctggaaagcc agccgctgtc ggcctggaag gcctatgcca agctgcgcgt
     2041 gctcaacgcc tacgcgccct atctgagcaa ggacttcgtc gacacccgct ttgccttcgt
     2101 cggcacggtg ctgcgcggca cgcccgagaa cctgccgcgc tggaagcggg gcgtgacgct
     2161 ggtcgagggc tcgctcggcg aaggcctggg caagctctac gtggcgcaat acttcccgcc
     2221 cgagagcaag gcccgcatgg aggagctggt ggccaatctg ctgacggcct acaagcagag
     2281 catagatagg ctcgattgga tgagcccggc gaccaagaag caggcccaga tcaagctcgc
     2341 caagttcacg cccaagatcg gctatcccaa gcgctggatc gactacagcg ccctggagat
     2401 caagcgcgac gacctcgcgg gcaatgtgcg gcgcgcccgc gagttcgagg tcaaccgcca
     2461 gctcgccaaa ctcggccggg cgatcgaccg cgacgagtgg ctgatgacgc cgcagacggt
     2521 caacgcctac tacaaccccg agctcaacga gatcgtcttc ccggcctcga tcctgcagcc
     2581 ccccttcttc gacgccaagg cggacgacgc ggtcaactac ggcggtatcg gcgccgtgat
     2641 cggccacgag atcagccacg gcttcgacga ccagggcagc cagtacgacg gcgacggcaa
     2701 tttgcgcgac tggtggacca agcaggaccg cgcgcgcttc aacgccaaga ccaagctgct
     2761 ggtcaagcaa tacggcgcct tcgagccggt gcccggctat ccggtcaacg gcgagctgac
     2821 actgggcgag aacatcgccg acaacgcagg gctggcgatc gcctacaagg cctacaagct
     2881 ctcgctgcac ggcaagcccg cgccggtgat cgacggcatg agcggcgacg agcgcttctt
     2941 ctacggcttc gcccaggtgt ggcgcggcaa gatgcgcgat gcggcgctga tccagcagct
     3001 caagtcggac ccgcatgcgc ccgacgagat tcgcgccaac ggcacggtgc gcaaccatcc
     3061 cggcttttat tcgaccttca agctcaagcc gggcgatgcg atgtacctgc cgccttccgg
     3121 gcgcgtgacg ctctggtgag agcgggcaag gcccgagcaa acggcgcctt cgggcgccgt
     3181 tttcgtttgg ctcgtctttt gtgacgggcg gctttctaga atcgggtgat ccttcaagcc
     3241 tcgtgttatg catccagacg tacaaattcc cgcacgctgc gaagtcctgg tcatcggtgc
     3301 cgggccggcc ggcagcgcct gtgcattgac gctggcccgc gccggactcg acgtggtgct
     3361 ggccgaccag catgcctttc cgcgcgacaa ggtctgcggc gacggactga tccccgatgc
     3421 ccatgccgcg ttgcgccgcc tgggcgtgct cgacgaggtg atggccgtcg cgcaggcggc
     3481 ccggcatgtg ggctgcgtgg gcccgcgcgg cgggcgcatc gatgtgcccg gcacgctggc
     3541 cgtgctgccg cggcgcgagt tggatcacat cctgtgccgc gcagcggtgc gcgccggcgc
     3601 gaagctgctg ccgccggcgc gcttcgaggc gccattggag cgcgacggca aggtcagcgg
     3661 cgcgcgcctg aaatgcggcg acggcacgct cgagatcgcg gccgattggc tcatcctcgc
     3721 caccggtgcc gtgccccagg ccatgatcgc cgccggcctg tgcacgcgcc gcacgcccag
     3781 cggcgtcgcg ctgcgcggct atgtgcgcaa cgaggccatg gcgaccaagc tcaaggcgct
     3841 ggacgtggtc tggcaccgca agctgcgccc cggctacggc tggatctttc cctgccgcga
     3901 tggggtgttc aacatcggcg tcggcctcgc gcacagccat agcaagttca gcaacggccg
     3961 ccgcaccatg caggacgtga acctgcgcga gaccttcgcc gccttctgcg agttctacga
     4021 accggcgcgc gagctgatgc aaggcggcac gctgctgggc gaactcaaag gcgcgcccct
     4081 gcgctgctcg ctcgaaggcg ccaaatactc ccgccctggc ctcattgcga ccggcgaggc
     4141 cgtcggcagc acctatgcct tcaccggcga gggcataggc aaggcgatgg aaaccggcat
     4201 gctggccgcc gaggccgtgc tgcagggacg acgcgagcag ctggacgagg cagccgtgcg
     4261 gcagcactac gagtctcaac tgcttgcgct caagccgcgc ttcgatcttt acgagaaagc
     4321 caaccgcgtc aacgagcacc cctggctggc cgatctgctg atctggcgcg ccaagcgcag
     4381 cgagcgcatc ctcaggcgca tgagcggcgt gctcgaaga
//
LOCUS       DASUCY010000477         3602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_460787, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000477 DASUCY010000000
VERSION     DASUCY010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460787"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(321..1526)
                     /gene="mmsB"
                     /locus_tag="VFV25_09490"
     CDS             complement(321..1526)
                     /gene="mmsB"
                     /locus_tag="VFV25_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HEY1393598.1"
                     /translation="MSAVPEVQAPDSVKSSRGDVLRFVKHHLREYGMLLSLVAIVGFF
                     QVMTSGTLLQPLNLTNLVQQNSYIVIMALGMLLVIVAGHIDLSVGSVCGFIGALAAVL
                     MVQHEWHFVPAALVCLAAGAAIGAAQGWFVAYFRIPSFIVTLAGMLVFKGLALWLLEG
                     QSVGPFPAEFQQLSSGFIPDVFAGSELRLSSLLLGVLVAVAMLIAAWRTRASAVGNGM
                     DVEPFVLFAARNLVFASAFVALSWLMASYRGLPNVLVVMFLLTVAYAFVATRTTVGRR
                     IYALGGNEKAARLSGIRTERLAFATFVNMGVLAALAGLVFAARLNTATPKAGLGFELD
                     VIAACFIGGASASGGVGKVIGAVIGAFVMGVMNNGMSILGIGIDVQQVIKGLVLLAAV
                     CFDVYNKNK"
     gene            complement(1510..3060)
                     /gene="mmsA"
                     /locus_tag="VFV25_09495"
     CDS             complement(1510..3060)
                     /gene="mmsA"
                     /locus_tag="VFV25_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEY1393599.1"
                     /translation="MTGLPPLLEMRGIRKTFPGVVALAGVDLSVQPGEIHAIVGENGA
                     GKSTLMKVLSGVYPHGSHEGEIRFEGQVRRFAVIADSERLGIIIIHQELALVPKLSIA
                     ENLFLGHEPARFGVIDRAQVHRRTRELLQRVGLDESPATLVTDLGVGKQQLVEIAKAL
                     AKDVKLLILDEPTASLNERDSDALLELLLELKRQRIACILISHKLNEVEKVADTITVL
                     RDGATVASFDCRRESPSQDLVIRHMVGREMADRYPRRTPKLGDTAFELRDWWVHHPAH
                     TDRPAVKGVSLRVRRGEIVGIAGLMGAGRTELAMSVFGRSWGKRIRGTALVNGRPVDV
                     STVRKAVSHGIAYVTEDRKSLGLVLDESITHNTTLANLKAVARRGVIDEGQEFTVAND
                     FRRKLATRCAGVHQPVVNLSGGNQQKVVLAKWLFADPTLLILDEPTRGIDVGAKFEIY
                     TLIAKLADEGRAILIISSEMPELLGLCDRIHVMNEGAFVAEFAGAQASQEKIMHAIVN
                     ASRNERRS"
     gene            complement(3057..>3602)
                     /locus_tag="VFV25_09500"
     CDS             complement(3057..>3602)
                     /locus_tag="VFV25_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY1393600.1"
                     /translation="YDGALSVFKPYLDSGKLVVRSRQLGMNKVGTLRWDGATAQARMD
                     NLLSAYYGKDRLHAVLSPYDGISIGILSSLKGVGYCTPQQPCPVVSGQDAEIPSVKSI
                     LRGEQSATVFKDTRELARVTADLVDTLLAGKAPQVNDTKTYQNGVKVVPAILLKPVAV
                     DSGNWKKVLVDGGYYKESQLK"
BASE COUNT          659 a         1214 c         1154 g          575 t
ORIGIN      
        1 atgtaggaac aaggctgaac acgtagccgg cgctcgaacc ggcgcgccgt taggatgctc
       61 tgaagcagcc acccgcgggg gaccgctaat ggccggcaag agccggccag cggacagatt
      121 cgacggtcag gtgagagcca ctgagcagcc gctcgagcgg caatgacgct ggggcagcgc
      181 gccaccctcg cgccaatcgc acgactgttc gttacttccc atcaagccat gcacctgacc
      241 tgcgcgccgc acgagatccg cggcgcagag ctcgcacggg tccgaatcgc tcggccacgc
      301 cgctcaggcg ccgcatcagt tcacttgttc ttgttgtaga catcgaaaca gaccgctgcg
      361 agcagcacga gccccttgat cacttgttgg acatctatgc cgatgccaag gatggacatg
      421 ccgttgttca tcacgcccat cacgaaggcg ccgatcacag cgcctatgac cttgccgact
      481 cctcccgagg ccgaggcacc gccgatgaag caggccgcga tcacgtcgag ctcgaacccc
      541 agccccgcct tcggcgtggc ggtgttcagg cgcgcggcaa acaccaggcc cgcgagcgca
      601 gcgagaacgc ccatgttcac gaaggtggca aaggccaggc gctcggtgcg gatgcccgac
      661 aggcgcgcgg ctttctcgtt gccgcccaac gcgtagatgc gccggcccac ggtcgtgcgt
      721 gtcgcgacga aggcgtaggc cacggtcagc aggaacatca ccacgagcac attcggcagg
      781 ccgcggtagg aggccatcag ccagctcaac gcgacgaagg cggaggcgaa tacgaggttg
      841 cgcgcggcaa agagcacgaa gggctcgacg tccatgccat tgccgacagc gcttgcgcgc
      901 gtgcgccagg ccgcgatcaa catcgcgacc gccacaagca cacccagcaa cagcgagctc
      961 aggcgcaact cgctgccggc gaacacgtcg ggaatgaagc ctgagctcaa ctgctggaac
     1021 tcggccggga acggcccgac cgactggccc tccagcagcc atagcgccag gcccttgaac
     1081 accagcatgc ccgccagcgt gacgatgaaa gagggaatgc ggaaataggc gacgaaccaa
     1141 ccctgcgccg cgcctatggc ggcgcccgcg gccagacaca ccagcgccgc gggcacgaag
     1201 tgccactcgt gctggaccat caacaccgcc gcgagggcgc cgatgaaacc gcagaccgag
     1261 ccaaccgaga ggtcgatgtg gccggccacg atcaccagca gcatgcccaa cgccatgatg
     1321 acgatgtagc tgttctgctg cacgaggttc gtcaggttga gcggctgcag cagcgtgccg
     1381 ctggtcatca cctgaaagaa gccgacgatc gccaccaggg aaagcagcat cccgtattcg
     1441 cgcaggtgat gcttgacgaa gcgcagcaca tcgccgcgcg aggacttcac ggaatcgggg
     1501 gcttgaactt caggaacggc gctcatttcg gctcgcattg acgatggcat gcatgatctt
     1561 ttcctgcgag gcctgcgcgc cggcaaactc cgcgacgaac gcaccttcgt tcatcacatg
     1621 gatgcggtcg cacaagccaa gcagctcggg catctcggaa gagatgatca ggatcgcccg
     1681 gccctcatcg gccagcttgg cgatcagcgt gtaaatctcg aacttcgcgc cgacgtcgat
     1741 gccgcgcgtg ggctcgtcca ggatcagcag cgtcgggtcg gcgaagagcc acttggcgag
     1801 cacgaccttc tgctgattgc cgccggagag attcaccacc ggctggtgca cgccggcgca
     1861 gcgcgtcgcg agctttcgcc ggaagtcgtt ggcgaccgtg aactcctggc cttcgtcgat
     1921 cacgccacgg cgggccacgg ccttcaggtt tgccagcgtg gtgttgtggg taatcgattc
     1981 gtcgagcacg aggccaagcg acttccggtc ctcggtgacg taggcaatgc cgtggctcac
     2041 cgccttacgc acggtcgaca cgtcgaccgg ccggccgttc accagcgcgg tgccgcggat
     2101 gcgcttgccc cagctgcgcc cgaacacgct catggcgagt tccgtgcggc cggcgcccat
     2161 caggcccgca atgcccacga tctcgccgcg gcgtacgcgc aggctcacgc ccttgaccgc
     2221 cgggcggtcg gtgtgcgccg gatggtgcac ccaccagtcg cgcagctcga acgcggtatc
     2281 gccgagcttc ggcgtgcggc gcggatagcg gtcggccatc tcgcgaccga ccatgtggcg
     2341 gatcaccaga tcctgcgacg gcgattcgcg tcggcaatcg aagcttgcga ccgttgcgcc
     2401 atcgcgcagc acggtgatcg tatcggcgac cttctcgacc tcgttgagct tgtgcgagat
     2461 caggatgcag gcgattcgct gccgcttcaa ctcgagcagc agttcgagca gcgcatcgct
     2521 gtcgcgctca ttcaggctcg ccgtgggctc gtcgaggatc agcagcttga catccttggc
     2581 gagcgccttg gcaatctcga cgagctgctg cttgcccacg cccaggtcgg taaccagcgt
     2641 cgccggcgac tcgtcgaggc ccacgcgctg cagcagctca cgcgtgcgac ggtgcacctg
     2701 cgcgcggtcg atcacgccaa agcgcgcggg ctcgtggccg aggaagaggt tctcggcgat
     2761 cgacagcttc ggcaccaggg ccagctcctg gtggatgatg atgatgccca ggcgttcgct
     2821 gtcggcgatg accgcgaagc ggcgcacctg gccctcgaag cggatctcgc cctcgtggct
     2881 gccgtgcggg taaacgcccg acagtacctt catcagcgtg gacttgccgg cgccgttctc
     2941 gccgacgatg gcgtggatct cccccggctg caccgaaagg tcgacaccgg ccagcgcaac
     3001 gaccccgggg aaggtcttgc ggatgccgcg catctccagc aatgggggca ggccggtcat
     3061 ttcagctgcg attccttgta gtagccgccg tccacgagca ccttcttcca gttgccagag
     3121 tccactgcca ccggtttgag caggatagcc ggcaccacct tgacgccgtt ctggtaggtc
     3181 ttggtgtcgt tgacctgcgg cgccttgccc gccagcaggg tatcgaccag atcggcggtg
     3241 acgcgcgcca gctcgcgcgt gtccttgaat acggtggcgc tttgctcgcc gcgcaggatg
     3301 gacttcaccg acgggatctc ggcatcctgc ccgctgacca ccgggcaggg ctgttgcggc
     3361 gtgcagtagc ccacgccctt gagcgaggac aggatgccga tcgagatccc gtcgtatggc
     3421 gacagcaccg cgtgcagcct gtccttgccg tagtaggccg acaataggtt gtccatgcgt
     3481 gcctgcgcag tggcgccgtc ccagcgcaag gtgccgacct tgttcatgcc cagctgcctg
     3541 gagcgcacga ccagcttgcc gctgtccagg tagggcttga acaccgacaa cgcgccgtcg
     3601 ta
//
LOCUS       DASUCY010000478         4338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_75348, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000478 DASUCY010000000
VERSION     DASUCY010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4338
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75348"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3384)
                     /locus_tag="VFV25_09505"
     CDS             complement(<1..3384)
                     /locus_tag="VFV25_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEY1393601.1"
                     /translation="MSNKANGRWQFWIDRGGTFTDIVAKHPQGHLLTHKLLSENPEQY
                     RDAAVAGIRHLLGLKPGEPITPAQVECVKMGTTVATNALLERKGEPTLLVITRGFRDA
                     LRIAYQERPRIFDRQILLPELLYARVIEAQERVGAQGDPIQALDEAQLRRELRSAFDA
                     GLRSVAIVFMHGYRYTAHERAAARIAREIGFTQVSASHETSPLMKLVSRGDTTVVDAY
                     LSPILLRYVQQVSAEMPGVRLLFMQSSGGLTDAHAFQGKDAILSGPAGGIVGMARTAQ
                     LAGFEHVIGFDMGGTSTDVSHYAGEFERAFETRVAGVRMRAPMMSIHTVAAGGGSILG
                     FDGARLRAGPESAGANPGPACYRRGGPLAVTDANVMLGKIQPAYFPKVFGPAADEALD
                     RAVVLRKFGELAERISQATGAPRSAEQVAEGYIDIAVGSMANAIKKISVARGYDVTRY
                     TLQCFGGAGGQHACRVADALGMSRVFAHPLAGVLSAYGMGLADQSLMREAAIEQPLDE
                     QAMPAIAARLDALEAQARAEIARQGVNAGALQAHRRAHVRYQGTDSALVVPFGSAAEI
                     QSAFEAAYLQRFAFLMLERSLVVEAVSVEAVASGEAPGEASHALQHSGAAPIAETVRL
                     YSGGRWWDAALVVREQARPGHVVDGPAIIAEKNATTVVEPGWQARVSEFDHLVLQRVQ
                     PREARKAIGTSVDPVMLEVFNNLFMNIAEQMGLQLQNTAYSVNIKERLDFSCALFDAE
                     GNLIANAPHMPVHLGSMSESIKTVMQRNAGRIRPGDVYALNDPYHGGTHLPDVTVVTP
                     VFDEAGKDILFYVGSRGHHADIGGITPGSMPPFSTRIDEEGVQIDNVKLVEAGRLLGD
                     EMLALLRSGPYPSRNPEQNLADLKAQIAANEKGVQELRKMVAQFGLEVVQAYMRHVQD
                     NAEESVRRVITRLKDGAYMLKLDNGAQIAVKIKVDAADRSAVIDFSGTSAQLANNFNA
                     PRAVCMAAVLYVFRTLVDDDIPLNAGCLKPLKVIIPEGCMLNPEPPASVVAGNVETST
                     CITNALYGALGVMASGQCTMNNFTFGNERHQYYETVSGGSGAGARIDARGEVAGGFDG
                     TSVVQTHMTNSRLTDPEVLEFRFPVRLESYEIRHGSGGAG"
     gene            complement(3390..3950)
                     /locus_tag="VFV25_09510"
     CDS             complement(3390..3950)
                     /locus_tag="VFV25_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HEY1393602.1"
                     /translation="MPTKPAKTRAKPAAREVAVAPLARGIVSSSHLVSPRSVESSEFE
                     FGLIVAWNAFAGWAVRCMAAAGCPDLTITDVTLLHHVSHRARNKKLADICFVLNYEDS
                     HVVAYSLKKLVAAGLVQTEKIGKEVFYSPTPQGEAHVAKYREVREACLVNNIDADLNR
                     QIGELARLLRTMSGLYDQAARAASSL"
     gene            complement(4045..>4338)
                     /locus_tag="VFV25_09515"
     CDS             complement(4045..>4338)
                     /locus_tag="VFV25_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEY1393603.1"
                     /translation="RRLPTEPEWELAACTLASRGFRHGEVWEWTASAARPYPGFMPGP
                     WREHSLAHFGAADHLVLRGGSFATRERIKHPRFRAFAAPVRDDLFSGFRSCAL"
BASE COUNT          696 a         1463 c         1474 g          705 t
ORIGIN      
        1 gccggccccg cccgagccgt ggcggatctc ataactctcc agccgcaccg ggaagcggaa
       61 ctcgagcacc tcgggatcgg tgagccgcga attggtcatg tgggtctgca cgacgctggt
      121 gccgtcgaag ccgcccgcga cctcgccccg cgcatcgatg cgcgcgccgg caccgctgcc
      181 gcccgagacg gtctcgtagt actggtggcg ttcgttgcca aaggtgaagt tgttcatcgt
      241 gcactggccc gaggccatca cgcccagggc gccgtagagt gcattggtga tgcaggtcga
      301 ggtctcgaca ttgcccgcca ctaccgaggc cggcggctcg gggttgagca tgcagccctc
      361 ggggatgatg accttcagcg gcttcaggca gccggcattg agcgggatgt cgtcgtccac
      421 cagcgtgcgg aagacataga gcaccgcggc catgcagacc gcgcgcgggg cattgaagtt
      481 gttggcgagc tgagccgagg tgccgctgaa gtcgatgaca gcgctgcggt cggcggcatc
      541 gaccttgatc ttcacggcga tctgggcccc gttgtcgagc ttgagcatat aggcgccgtc
      601 cttcagccgc gtgatcacgc ggcgcaccga ttcctcggcg ttgtcctgca cgtgccgcat
      661 ataggcctgc acgacctcga ggccgaactg cgcaaccatc ttgcgcagct cctgcacgcc
      721 cttctcgttg gcggcgatct gggccttgag atcggccagg ttctgctcgg gattgcgcga
      781 cggatagggg ccgctgcgca gcagggccag catctcatcg ccgagcaggc gcccggcctc
      841 gaccagcttg acattgtcga tctgcacacc ctcttcgtcg atgcgagtcg agaacggcgg
      901 catcgagccc ggcgtgatgc cgccgatgtc ggcatggtgg ccgcgcgagc cgacgtagaa
      961 gaggatgtct ttgccggcct cgtcgaacac gggggtgacc accgtcacgt cgggcagatg
     1021 cgtgccgccg tgatagggat cgttgagcgc gtagacgtcg ccgggcctga tgcgccccgc
     1081 attgcgctgc atcacggtct tgatcgattc gctcatcgag cccaggtgca ccggcatatg
     1141 cggggcgttg gcgatcaggt tgccttcggc atcgaagagc gcgcaggaga agtccagcct
     1201 ctccttgata ttgaccgagt aggccgtgtt ctgcagctgc aggcccatct gctcggcgat
     1261 gttcatgaag aggttgttga agacctccag catcaccggg tccacgctcg tgcctatggc
     1321 tttgcgcgct tcgcgcggct gcacacgctg cagcaccaga tggtcgaact cgctcacgcg
     1381 ggcctgccag cccggctcga cgacggtggt ggcgttcttc tccgcgatga tggccggccc
     1441 gtcgactacg tggccggggc gggcctgctc gcgcaccacc agggccgcgt cccaccagcg
     1501 cccaccgctg tagagccgca ccgtttcggc gatgggcgcg gcgccgctgt gctgcaaggc
     1561 atggctggct tcgcccggcg cttcgcccga ggccacggcc tcgaccgaca ccgcctccac
     1621 caccaggctg cgctcaagca tcaggaaggc gaagcgctgc aggtaggccg cctcgaaggc
     1681 gctctgaatc tcggccgccg agccgaaggg cacgaccagc gccgaatccg tgccctggta
     1741 gcgcacatgg gcgcgccggt gcgcctgcag cgcacctgca ttcacgcctt ggcgcgcgat
     1801 ctcggcgcgg gcctgggctt ccagcgcgtc gagcctggcg gcgatggccg gcatcgcctg
     1861 ctcgtcgagc ggctgctcga tggccgcctc gcgcatcagg ctctggtcgg ccaggcccat
     1921 gccataggcc gaaagcacgc cggcaagcgg atgcgcgaag acgcggctca tacccagcgc
     1981 atcggccacg cggcaggcgt gctggccgcc agcgccgccg aagcattgca gcgtgtagcg
     2041 cgtcacgtcg tagccgcgtg ccaccgagat cttcttgatc gcattggcca tgctgccgac
     2101 ggcgatgtcg atataaccct cggccacttg ctcggcgctg cgcggcgctc cggtggcctg
     2161 cgagatgcgc tcggccagct cgccgaactt gcgcagcacg accgcgcggt ccagcgcctc
     2221 gtcggccgcg gggccgaaga ccttggggaa ataggccggc tggatcttgc ccagcatcac
     2281 gttcgcatcg gtcacggcca aggggccccc acgccggtag caggccgggc cgggattggc
     2341 gcccgcgctc tcggggccgg cgcgcaggcg cgcgccgtcg aagccgagga tggagccgcc
     2401 gcccgccgcc accgtgtgga tgctcatcat cggcgcgcgc atgcgcacgc cggccacacg
     2461 cgtctcgaag gcgcgctcga actcgccggc gtaatgcgag acatcggtcg aggtgccgcc
     2521 catgtcgaag ccgatcacat gctcgaagcc ggccagctgc gcggtgcgcg ccatgccgac
     2581 gatgccgccc gccgggccgg acaggatggc gtccttgccc tggaaggcgt gggcatcggt
     2641 gaggccaccc gaagattgca tgaaaagcag ccgtacccca ggcatttccg ccgaaacctg
     2701 ctgcacataa cgtagcagga tcggcgacag ataggcgtcc accactgtcg tgtcgccgcg
     2761 actcaccagc ttcatcagcg ggctggtctc gtgcgaggcg ctcacctgcg tgaagccgat
     2821 ctcgcgcgcg atgcgcgccg ccgcccgctc gtgcgcggtg tagcgatagc cgtgcatgaa
     2881 gacgatggcc acgctgcgca ggccggcgtc gaaggcggat cgcagctcgc ggcgcagctg
     2941 cgcctcgtcc agcgcctgga tcggatcgcc ctgcgcgccg acgcgctcct gcgcctcgat
     3001 gacgcgcgca taaagcagct cgggcagcag gatctggcga tcgaagatgc gcgggcgttc
     3061 ctggtaggcg atgcgcaagg cgtcgcgaaa gccgcgcgtg atgacgagca gcgtgggctc
     3121 gcccttgcgc tccagcagcg cattggtcgc cacggtcgta cccatcttca cgcattcgac
     3181 ctgggccggc gtgatcggct cgcctggctt aaggcccagc agatgacgga tgccggccac
     3241 ggccgcgtcg cggtattgct cggggttctc gctcagcagc ttgtgcgtga gcagatgccc
     3301 ctgcggatgc ttggcgacga tgtcggtaaa ggtaccgccg cggtcgatcc agaactgcca
     3361 gcggccgttt gctttattgc tcatggtgtt tagagggaag aggcggcacg cgccgcctgg
     3421 tcataaaggc cggacatggt gcgcagcagc cgcgccagct cgccgatctg ccggttcagg
     3481 tcggcgtcga tgttgttgac gaggcaggcc tcgcgcacct cgcggtactt cgccacatgg
     3541 gcctcgccct gcggcgtcgg gctgtagaag acttccttgc cgatcttctc ggtctgcacc
     3601 aggcccgcgg cgaccagctt cttcagcgaa taggccacca catggctgtc ctcgtagttc
     3661 agcacgaagc agatgtccgc gagcttcttg ttgcgcgcgc ggtggctgac gtgatgcagc
     3721 agcgtcacgt cggtgatcgt cagatcggga cagccggcgg ccgccatgca tcgcaccgcc
     3781 cagcccgcga aggcattcca ggccacgatg aggccgaact cgaactccga ggactcgacg
     3841 ctgcgcggcg agactagatg cgaggaggac acgatgccgc gcgccagcgg ggcgaccgcg
     3901 acttcgcgag cggccggctt ggcgcgtgtc ttggcgggct tggtaggcat gctgatgcgc
     3961 caatacgttg ataatttatc aacgttttat cctaaaaaag ccgcgatatc aacccggctc
     4021 atcccgcccg gacaaaaaag ccgctcaaag cgcgcaggag cgaaagccgc tgaacagatc
     4081 gtcccgcacc ggcgcggcaa aggcgcgaaa gcgcgggtgc ttgatgcgct cgcgcgtggc
     4141 gaagctgccg ccgcgcagga ccaggtgatc ggccgcaccg aaatgcgcca gcgagtgctc
     4201 gcgccagggc ccaggcatga aacccggata gggccgcgcc gccgaggccg tccattccca
     4261 cacctcgcca tggcggaagc cgcggctggc cagggtacag gcggccagct cccactcggg
     4321 ttcggtcggc aggcgtcg
//
LOCUS       DASUCY010000479         2492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_203515, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000479 DASUCY010000000
VERSION     DASUCY010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203515"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1882
                     /gene="fusA"
                     /locus_tag="VFV25_09520"
     CDS             <1..1882
                     /gene="fusA"
                     /locus_tag="VFV25_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEY1393604.1"
                     /translation="DPQERQRGITINSAAITVHWKGAQINLIDTPGHIDFNIEVNRSL
                     RVLDGAVVVFDGVAGVEPQTETNWRLADRYQVPRIAFINKLDRVGADFSRVVAMMEER
                     LGARVLPLQLPIGAEGDFRGVVDLLTMRALLWEKDDASPYRETDVPADLLDAARRQRE
                     RLVELVAEQDEAALHAYLNGDAVDQALLRAGIRKGVLAGAFVPALAGAAFKNRGIEPL
                     LDAVVDYLPTPAEVRRPEGEPASDPNGPFAALAFKLTTDDHGAMVFVRVYRGRLRRGD
                     TVLNASTGRTERVTRVYEVHADDHIERDGLEAGDIAAIVGLKDTLTGHTLCDRDHPVH
                     LEEISVPEPVIHVAIEPRAQGDLQGLVKALQAMLREDPSLRVRQDAESGQTILSGMGE
                     LQLEVSIEKLRARHGVNVLVGRPQVAYRETITRSVEVSHVHKKQTGGPGQFAELRLRL
                     EPLARGAGIRFDSRIVGGAVPREFIPAVEQGIRRAAQAGVLAGFPCVDFQATLVDGSF
                     HERDSSTLAFELAAAAAFREAFLQAGPQMLEPVMSVEVLTPVDYLGDVIGDLHRRRGL
                     VRGQGQRGNTAVVDARVPLKEMFGYIGSLRALSSGRAQYSMQLDHYDAAPASETAELL
                     QG"
     gene            complement(1946..2317)
                     /locus_tag="VFV25_09525"
     CDS             complement(1946..2317)
                     /locus_tag="VFV25_09525"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY1393605.1"
                     /translation="MLDHISLGTHRYAEAVAFYSAVLAPLRLTLQRDTGREASFGTQE
                     RWMFFLYPVPPGESVAAQGMHLAFGAASRRQVEQVHAGALAASATDIFTPRARPDISP
                     IYFGAMFHDLDGHRIEVKTDA"
BASE COUNT          389 a          808 c          915 g          380 t
ORIGIN      
        1 cgacccgcag gagcgccagc ggggcatcac gatcaacagc gcggcgatca ccgtgcattg
       61 gaagggcgcg cagatcaacc tgatcgacac gcccggccac atcgacttca acatcgaggt
      121 gaaccgctcg ctgcgcgtgc tggacggcgc ggtcgtggtc ttcgatggcg tggccggcgt
      181 ggagccgcag accgaaacca actggcgcct ggcggacagg taccaggtgc cgcgcatcgc
      241 cttcatcaac aagctcgacc gcgtgggggc tgatttctcg cgcgtcgtcg cgatgatgga
      301 agagcggctc ggcgccaggg tgctgccgct gcagctgccc atcggcgcgg aaggcgactt
      361 ccgcggcgtc gtcgacttgc tgacgatgcg cgcgctcctg tgggagaagg acgatgccag
      421 cccctatcgc gaaacggacg tgcccgccga cttgctcgac gccgcgcggc gtcagcgcga
      481 gcggctggtg gagctggtcg ccgagcagga cgaggcggcg ttgcatgcct atctgaacgg
      541 cgatgctgtc gaccaggcac tgctgcgcgc gggcatccgc aagggagtgc tcgcgggggc
      601 cttcgtcccg gcattggcgg gtgctgcgtt caagaaccgc gggatcgagc cgctgctcga
      661 cgccgtggtg gactatctgc cgacacctgc cgaggtacgc cggccggaag gcgagcctgc
      721 atccgatccg aacgggccgt tcgcggcctt ggcgttcaag ctcacgaccg acgaccacgg
      781 tgcgatggtc ttcgtacgcg tctaccgcgg ccggctgcgc cgcggcgaca cggtgctcaa
      841 tgcgagcacc ggccgcaccg agcgcgtcac gcgcgtgtac gaagtgcatg ccgacgacca
      901 catcgagcgc gacgggctcg aggcgggcga catcgcggcg atcgtcgggc tgaaggacac
      961 gctgaccggc cacaccttgt gcgaccgcga ccatccggtc cacctggagg aaatcagcgt
     1021 tcccgagccg gtgatccatg ttgcgattga gccgcgcgcc cagggcgatc tgcagggcct
     1081 agtcaaggcc ttgcaggcga tgctgcgcga ggatccgagc ctgcgcgttc ggcaggatgc
     1141 cgagtcgggg cagaccatcc tctccggcat gggcgagctg cagctcgagg tgtcgatcga
     1201 gaagctgcgc gcccgccacg gcgtgaatgt gctggtggga cgcccgcaag tggcctaccg
     1261 ggaaacgatc acccggagcg tggaggtgag ccatgtgcac aagaagcaga ccggcggccc
     1321 cggccagttc gccgagctga ggctgcgcct ggagccgctg gcccgaggcg caggcatacg
     1381 cttcgacagc cggatcgtcg gcggcgcagt gccccgcgag ttcatccccg cggtggagca
     1441 gggcatccgg cgtgcggccc aggccggcgt gctggcgggc ttcccctgcg tggacttcca
     1501 ggccacgctg gtggacggca gcttccacga gcgcgactcg tcgacgttgg ccttcgagct
     1561 ggcggcggcc gctgccttcc gcgaggcctt cctgcaagca gggccgcaga tgctggagcc
     1621 cgtgatgagc gtggaggtgc tgacgccggt cgattacctg ggtgacgtca tcggcgacct
     1681 gcatcgcagg cgcggccttg tgcgtgggca gggtcagcgc ggcaacacgg cggtcgtcga
     1741 tgcccgggtg ccgctgaagg agatgttcgg ctacatcggc agcctgcgtg cgctctcgtc
     1801 cgggcgtgcg cagtattcga tgcagctgga ccactacgac gcggcgccgg ccagcgagac
     1861 ggccgagctc ttgcagggct gagttgccgt tgaggagggg cgtggcctgg cgggccacgc
     1921 cctcttcggt cctcaccgcg agccttcagg cgtcggtctt cacctcgatg cggtgcccgt
     1981 ccaggtcgtg gaacatcgcg ccgaagtaga tggggctgat gtccggccgg gcgcgtggcg
     2041 tgaagatatc ggtggccgag gcagcgagcg cgccagcgtg gacttgctcg acctggcggc
     2101 gagaggcggc gccgaatgcc aggtgcatgc cttgtgcagc gacgctttcg cccggcggca
     2161 ccgggtagag aaagaacatc caccgctcct gcgtgccgaa gctagcctcg cggccggtat
     2221 cgcgctgcag ggtcagcctc agtggggcca gcacggcgct gtagaaggcg acggcctcgg
     2281 cataacggtg cgtgccgagc gagatgtgat cgagcatggg gctcctttgc ttcgaatgac
     2341 agggggaaca aaaggcgttc agcctagcca gcgcgcgcct gctgctcgga actgatgttg
     2401 ctgccgcaca gcacggtggc cacgcgctgc cggcgcagtg gggggtgttc gatcaccgcg
     2461 gccacgccgg cagcgccggc cggctcgacc ac
//
LOCUS       DASUCY010000480         2838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_508929, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000480 DASUCY010000000
VERSION     DASUCY010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508929"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VFV25_09530"
     CDS             complement(<1..333)
                     /locus_tag="VFV25_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEY1393606.1"
                     /translation="MKLKSPMPACLLVCAVAAAGPVHAQERRNAFDDPFVQVTAALPG
                     CPVPEGPLYTEQEVHDVAHVRAQHGGSCYRAGRCRLPNSYLYDKEIIPRVQQYLRLDG
                     RFNDTSVWV"
     gene            complement(405..1334)
                     /locus_tag="VFV25_09535"
     CDS             complement(405..1334)
                     /locus_tag="VFV25_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEY1393607.1"
                     /translation="MVTKKPKGLGRGLEVLLGPKVSESASADNAGSSATSPGLPASLR
                     LVDLVAGQYQPRTRMDEGALYELAESIKVQGIMQPILVRKLGAGANTGKYEIIAGERR
                     FRAARLAGLDSVPVLVRDVPDESAAAMALIENIQREDLNPLEEAQGLQRLVREFGLTH
                     ELAAQAVGRSRSAASNLLRLLNLADPVQTMLMAGDLDMGHARALLALERATQITAANQ
                     ITAKKLSVREAESLVKKLGAEFSLKPQKAAKEKSRDTRRVEEELADLLMAEVEVRIKK
                     QVKRNGRREDMGEVAIQFASMDELNGLIERLRG"
     gene            complement(1384..2670)
                     /locus_tag="VFV25_09540"
     CDS             complement(1384..2670)
                     /locus_tag="VFV25_09540"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEY1393608.1"
                     /translation="MNSRTNARLARMQCFFFHAALALLVLASPAARAEPDEDVLGKAR
                     DYPLGTTSTWYGNPFRVGSWSALDKVPGMRTRLVPRAAEASPLPHAAQPPAISYRYKN
                     TRYTLADYLERQRATGLLVLQRGEIIAEHYRYGRQDDARFLSFSMAKSVTSLLIGQAH
                     ARGQIASLDDPAEKYVKALAGSPYGTATIRQLLRMSSGLTFTERYDGQDDVARLARAS
                     AGAAGAGAPVDVLRSITERHSPAGAKFVYASAETDVLGRVLTAATGKNMAELTSEWLW
                     KPMGAEHDAFWRVASDGQEQSFGGFNASLRDWARLGLLLANDGKVGSLQVVPRDYLLD
                     ATDPARQPPAFKPRKATPYFGYGYQFWLLPLRERSFAMQGIHGQTVYVQPSTGVVMVL
                     TSVWESASGRQNPRPYEERDALWRGVLQSLGGSTFE"
     gene            complement(2667..>2838)
                     /locus_tag="VFV25_09545"
     CDS             complement(2667..>2838)
                     /locus_tag="VFV25_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY1393609.1"
                     /translation="APERARQFAAAWGAELIDAGPRGHLNAESGLGDWPLAHEQLQRL
                     QGLPASTCRDTH"
BASE COUNT          416 a          943 c          971 g          508 t
ORIGIN      
        1 cacccagacg ctggtgtcat tgaaacgccc gtccagccgc aggtactgct gcacgcgcgg
       61 aatgatctcc ttgtcgtaca ggtaggagtt gggcagccgg cagcggccgg cacggtagca
      121 gctgccgcca tgctgggccc gcacgtgggc cacgtcgtgg acctcttgct cggtgtacaa
      181 cgggccttcg ggcaccgggc agcccggcag ggccgccgtc acctggacaa agggatcgtc
      241 gaacgcattc cggcgctcct gcgcgtgcac cggcccggcc gcagccaccg cgcacaccag
      301 caggcaggca ggcatcggcg atttcagctt catgattaac ttaacgcgcc tggttcatca
      361 agcccgttac agtgcgccgg ccccggcggt ctgtcaagcc gccatcagcc gcgcagccgc
      421 tcgatcagcc cgttcaactc atccatcgag gcaaactgga tcgccacctc gcccatgtct
      481 tcgcgccggc cgttgcgttt gacctgcttt ttgatgcgca cttcgacctc ggccatcagc
      541 aggtcggcca gctcttcttc gacacggcgg gtgtcgcgcg acttctcctt ggcggctttt
      601 tgcggcttca gcgagaactc ggcgccgagt tttttgacca ggctttcggc ctcgcgcacc
      661 gacagttttt tggccgtgat ctggttggcg gcggtgattt gcgtggcacg ctccagggcc
      721 agcagggccc gcgcatggcc catgtccagg tcgccggcca tcagcatggt ttgcaccggg
      781 tcggccagat tcaaaaggcg cagcaggttg gaggcggcac tgcgggaacg ccccacggcc
      841 tgcgccgcca gctcgtgagt gagcccgaac tctctcacca ggcgctgcaa accttgggcc
      901 tcttccaatg ggttgaggtc ttcgcgctgg atgttttcga tcaaggccat ggccgcggcc
      961 gactcgtcgg gcacgtcgcg caccagcacc ggcacgctgt ccaggcccgc gagccgggcc
     1021 gcgcggaatc gccgttcacc ggcgatgatt tcgtacttgc cggtgttggc gcccgcgccg
     1081 agcttgcgca ccaggatggg ctgcatgatg ccctgcacct tgatggactc ggccagctcg
     1141 tagagcgcgc cctcgtccat gcgggtgcgc ggctggtact ggccggccac caagtcgacc
     1201 aggcgcagcg aggccggcag gccggggctg gtggcactgc tgccggcatt gtccgcgctc
     1261 gcggattcgc tgactttggg gccgagcagg acttccagtc cgcggcccag gcctttgggt
     1321 tttttcgtga ccatggtttg tttcttttct ctcgatcttg agtcggtgag tgatgtcggt
     1381 ggattattcg aaagtgctgc cgcccagcga ctgcagcacg ccgcgccaga gcgcgtcgcg
     1441 ttcttcatac ggccgtggat tttgccggcc gctggcgctt tcccagaccg aggtcagcac
     1501 catcaccaca ccggtcgatg gctgcacata caccgtctgg ccatggatgc cctgcatggc
     1561 aaagctgcgc tcacgcagcg gcagcaacca gaactggtag ccgtagccga agtagggcgt
     1621 ggccttgcgc ggcttgaagg ccggcggctg gcgcgcggga tcggtggcgt cgagcaggta
     1681 gtcgcgcggc accacctgca ggctgcctac cttgccgtcg ttcgccagca gcagccccag
     1741 gcgagcccag tcgcgcaggc tggcgttgaa gccgccaaag gattgctcct gcccgtcgct
     1801 ggcgacacgc cagaaagcgt cgtgctcggc ccccatgggc ttccacagcc attcgctggt
     1861 gagctcggcc atgtttttgc cggtggccgc cgtcagcaca cggcccagca catcggtctc
     1921 ggcactggca tagacgaact tcgcgcccgc cggtgaatgg cgttccgtga tgctgcgcag
     1981 cacgtcgacc ggggcaccgg caccggccgc cccggcgctg gcgcgcgcga gccgggcaac
     2041 gtcgtcctgg ccgtcgtagc gctcggtaaa cgtcaggccc gaactcatgc gcagcagctg
     2101 gcgaatggtg gcggtgccgt aggggctgcc ggccagtgcc ttgacgtatt tttccgccgg
     2161 gtcgtcgagc gaggcgatct ggccgcgcgc atgggcctgg cctatgagca aggaggtgac
     2221 gctcttggcc atggagaacg acaaaaagcg cgcgtcgtcc tggcgcccgt agcggtagtg
     2281 ctcggcgatg atctcgccgc gttgcagcac cagcaggccg gtggcgcgct ggcgctccag
     2341 gtaatcggcc agggtgtagc gggtgttttt atagcggtag gagatggccg gcggctgcgc
     2401 ggcgtgcggc aaggggctgg cctcggcggc gcgtggcacc agccgggtcc gcatgccggg
     2461 aaccttgtcc agcgccgacc aggagccgac acgaaacggg ttgccatacc aggtgctggt
     2521 ggtgcccagc ggatagtctc gcgccttgcc gagcacgtct tcgtcgggct cggcccgggc
     2581 cgccgggctg gccagcacga gcagtgccag cgcggcatga aaaaagaagc attgcatgcg
     2641 ggccaggcgg gcgttcgttc tgctgttcaa tgggtgtccc ggcatgtcga ggccggcagg
     2701 ccttgcaggc gctgcagctg ttcgtgcgcc agcggccagt cgcccagccc gctttcggca
     2761 ttgaggtggc cgcgcgggcc ggcgtcgatc agctcggcgc cccaggcggc ggcgaactgc
     2821 cgggcccgct ccggcgcg
//
LOCUS       DASUCY010000481         2429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_605044, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000481 DASUCY010000000
VERSION     DASUCY010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605044"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1299)
                     /gene="argH"
                     /locus_tag="VFV25_09550"
     CDS             complement(<1..1299)
                     /gene="argH"
                     /locus_tag="VFV25_09550"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEY1393610.1"
                     /translation="MSSNQLDKKAQAWSALFSEPMSELVKRYTASVSFDQRLWQADIE
                     GSLAHAEMLAAQGIIAAEDYAAIQRGMVQIKGEIESGRFEWKLDLEDVHLNIEARLTQ
                     LVGDAGKRLHTGRSRNDQVATDVRLWLRGEIDGLAPLLAQMQGALLDVAEKNVETILP
                     GYTHMQVAQPVSFAHHLLAYVEMFKRDAERLADVRKRVNQLPLGAAALAGTSYALDRE
                     RVAKTLGFEGICQNSLDAVSDRDFALEFASFASITMVHVSRLAEEIVLWMSQNFGFID
                     LADRYCTGSSIMPQKRNPDVAELARGKSGRVVGHLMGLITLMKGQPLTYNKDNQEDKE
                     PLFDTVDTVRDTLRIMSEMVGGITVKPEAMERAALKGYATATDLADYLAKKGLPFRDA
                     HEVVAHAVKLGLQRGVDLSELPLETLQGFHPSIAEDVYQVL"
     gene            1411..>2429
                     /locus_tag="VFV25_09555"
     CDS             1411..>2429
                     /locus_tag="VFV25_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY1393611.1"
                     /translation="MPDPLPTTGFGSQSRPGDLSSVGGSSGFGTTILDVAPPPKSAPS
                     SAFDVCHVGVVLRAVLFVHGVAAIAVCFVSSGPSDWLFSFATASAFALPALLLWLVVA
                     CLCKSRLANLPDAAQWAAAVLLGALSGAYAWGLLLLTGAAPGGGHWRWLAPVLSAAAM
                     AAAIFHWLRLRARSELPAATVAKLAELQSRIRPHFLFNTLNTAIALVRLDPARAEAVL
                     EDLSELFRVALNDSDAAVTLAEEVELAQRYLAIEQIRFGSRLSVSWELDPAAGAAKLP
                     PLLLQPLVENAVRHGVEPSAEGGAIRVRTRLRRGHAIVSVSNSLPGLPSQPGRGMALR
                     NVRERL"
BASE COUNT          329 a          835 c          833 g          432 t
ORIGIN      
        1 cagcacctga tacacgtctt ccgcgatcga agggtggaag ccctgcagcg tttccagcgg
       61 cagctcgctc aggtcgacgc cgcgctgcag ccccagcttc accgcatgcg cgacgacctc
      121 gtgcgcgtcg cggaagggca agcccttctt cgccaggtag tccgccagat cggtcgcggt
      181 ggcatagccc ttcagcgcgg cgcgctccat ggcctcaggc ttgaccgtga tgccacccac
      241 catctcgctc atgatgcgca gcgtgtcgcg caccgtgtcc acggtgtcga agagcggctc
      301 cttgtcttcc tggttgtcct tgttgtaggt cagcggctgg cccttcatca atgtgatcag
      361 gcccatcagg tggccgacca cgcggccgct cttgccgcgg gcgagctcag cgacgtcggg
      421 attgcgcttc tgcggcatga tcgacgagcc ggtgcaatag cggtcggcca ggtcgatgaa
      481 gccgaagttc tggctcatcc acagcacgat ctcctcggcc aggcgcgaga cgtgcaccat
      541 cgtgatcgac gcgaaggacg cgaactcgag cgcgaagtcg cggtcgctca ccgcgtccag
      601 gctgttctgg cagatgccct cgaagcccag cgtcttcgcg acgcgctcgc ggtccagcgc
      661 atagctggtg ccggccagcg cggcggcgcc cagcggcagc tggttcacgc gcttgcgcac
      721 atcggccagg cgctcggcgt cgcgcttgaa catctccaca taggcgagca gatggtgcgc
      781 gaagctcacc ggctgggcca cctgcatgtg ggtgtagccg ggcaggatgg tctcgacgtt
      841 cttctcggcc acgtcgagca gcgcgccttg catctgggcc agcagaggcg ccaggccgtc
      901 gatctcgccg cgcagccaca ggcgcacgtc ggtggcgacc tggtcgttgc gcgagcgtcc
      961 tgtgtgcaat cgcttgccgg catcgcccac cagctgcgtg agccgggcct cgatgttgag
     1021 gtgcacgtcc tccaggtcga gcttccattc gaagcggccg gactcgatct cgcccttgat
     1081 ctgcaccatg ccgcgctgga tggctgcata gtcctccgcg gcgatgatgc cctgcgcggc
     1141 cagcatctcg gcgtgtgcga ggctgccttc gatgtccgct tgccacaggc gctgatcgaa
     1201 gctcacgctg gccgtgtagc gcttgacaag ctcgctcatc ggctcggaga acagggcgga
     1261 ccaggcttgc gccttcttgt ctagttgatt ggagctcatg gaggaacaga cggttgggcg
     1321 cgggcttgcc gcgcggcgtg cggcttggag acaatggatt gtaggtaagc tcgcgctcgc
     1381 cgctgtctgc agctctgtcc cccatcatcg atgcccgacc ccttgcccac caccggcttc
     1441 ggcagccaaa gccgccccgg cgacctgtcc agcgtcgggg gcagcagcgg cttcggcacc
     1501 accatcctcg acgtggcgcc gccgcccaag agcgcgccga gctcggcctt cgacgtctgc
     1561 catgtgggcg tggtgttgcg cgccgtgctc ttcgtacatg gcgtggctgc catcgccgtg
     1621 tgtttcgtca gcagtggccc cagtgattgg ctgttcagct tcgccaccgc gtcggccttc
     1681 gccttgccgg ccctgctgct gtggctggtg gtggcctgct tgtgcaagtc gcgcctggcc
     1741 aacctgcccg atgcggcgca atgggccgcg gcggtgctgc tgggggcgct gagcggcgcc
     1801 tatgcctggg gcctgctgct gctcaccggc gccgcgccgg gcggcggcca ttggcgctgg
     1861 ctcgcgccgg tgctgagcgc cgccgcgatg gccgcggcga tctttcactg gctgcgctta
     1921 cgcgcgcgca gtgagctgcc ggcggccacg gtggccaagc tggccgagct gcagtcgcgc
     1981 atccgcccgc acttcctttt caacacgctc aacacggcaa tcgcgctggt gcggctggac
     2041 ccggcgcgtg ccgaggcggt gttggaggac ttgtccgagc tgttccgcgt ggcactgaac
     2101 gacagcgacg ccgccgtgac cctggccgag gaggtggaac tggcccaacg ctatctggcg
     2161 atcgagcaga tccgcttcgg cagccggctc agcgtgagct gggagctcga cccggcggcc
     2221 ggcgcggcga aactgccccc gctgctgctg cagccgctgg tggaaaacgc cgtgcgccat
     2281 ggcgtggagc ccagcgccga gggcggggcg atacgcgtgc gcacgcggct gcggcgcggc
     2341 catgccatcg tgtcggtgag caacagcctg ccgggcctgc cctcgcagcc gggccgcggc
     2401 atggccttgc gcaatgtgcg cgagcgcct
//
LOCUS       DASUCY010000482         2452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_573222, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000482 DASUCY010000000
VERSION     DASUCY010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573222"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VFV25_09560"
     CDS             complement(<1..542)
                     /locus_tag="VFV25_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY1393612.1"
                     /translation="MIARLIRWSIANRFLVLLATLMVSAWGVYSVMKTPLDALPDLSD
                     VQVIIRTTYPGQAPRIVENQITYPLTTTMLSVPGAKTVRGYSFFGDSFVYVLFEDGTD
                     LYWARSRVLEYLNQVQSRLPPTAKASLGPDATGVGWIYQYALVDHSGTQDVSQLRALQ
                     DWFLKYELKTVPNVAEVATIG"
     gene            complement(539..2173)
                     /locus_tag="VFV25_09565"
     CDS             complement(539..2173)
                     /locus_tag="VFV25_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEY1393613.1"
                     /translation="MKTTSIAVAVAAVVIVAASGYGLYRLGMHRGMQDAPVTATTAEP
                     AAPAAAQGRLPQSTAEGEEATRRHMKAGLKAGDVDPMTGRKILYYHDPMVPGNKFDVP
                     SKSPFMDMMLVPVYEGAGGDEGKITVSPRIQQNLGLRTAEVTEGTLSPQVSAVGGIAF
                     NERDQVIVQARATAYVERLYVRATLDPVRKGQPLAELYVPDWIAAQEEFLSVRRMQGG
                     DLAPLLDGARQRMRQVGMSEEQIRLVESSGKTQPRITLTAPIGGVVVELMAREGMTVM
                     SGATLFRINGLGTVWANAEVPESQAALLRPGAKVQAKSPAVPGMSFDGRVQAILPEVN
                     AATRTLKARMELANSGSRLAPGMFVTMNFMDVRPEKALLVPSDAVIQTGRRTVVMLAE
                     DNGHFRPVEVQAGIESGGQTEIKRGLQVGQRVVVSSQFLIDSEASLRGLEARLNDAPA
                     SQVAAAPRYEGEGKVEAIGRDALTISHGPIPALKWGPMTMDFKLPARKLPRGLEAGDR
                     IGFEFTMGADGLPQLTSVQLQAPEPSAASAADGSGR"
     gene            complement(2170..>2452)
                     /locus_tag="VFV25_09570"
     CDS             complement(2170..>2452)
                     /locus_tag="VFV25_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY1393614.1"
                     /translation="VAEARAQLQQWGSNRQRLQRYEAELLPLAVQRTQAALAAYRGGG
                     TPLAAVLDARRAEIDTRMDQLKLEMETARLWAQLNYLVPAAHDSSENPR"
BASE COUNT          370 a          866 c          804 g          412 t
ORIGIN      
        1 ccgatcgttg cgacctcggc cacattgggc accgtcttca gctcgtactt caggaaccag
       61 tcctgcagcg cgcgcagctg cgacacgtcc tgcgtgccgc tgtggtccac cagggcgtat
      121 tggtagatcc agccgacgcc cgtggcatcc gggccgagcg atgccttggc ggtcggcggc
      181 aggcgcgact gcacctggtt caggtactcc agcacgcgcg agcgcgccca gtacaagtcc
      241 gtgccgtcct cgaagagcac gtaaacgaac gagtcaccga agaacgagta gccgcgcacc
      301 gtcttcgcgc ccggcaccga cagcatcgtc gtcgtcaacg ggtaggtgat ctggttctcg
      361 acgatgcgtg gcgcctgacc cgggtaggtc gtgcggatga tcacctgcac atccgacagg
      421 tcaggcagcg catcgagcgg cgtcttcatc accgagtaca cgccccaggc gctgaccatc
      481 aacgtggcca gcagcacgag gaagcggttg gcgatcgacc agcggatgag gcgggcgatc
      541 atcggccgct cccgtcggcc gcagaagccg cgctcggttc gggcgcctgc agctgcacgc
      601 tggtgagctg cggcaggccg tccgcgccca tcgtgaattc gaagccgatg cggtcgccgg
      661 cctccaggcc gcgcggcagc ttgcgcgcgg gcagcttgaa gtccatcgtc atcggccccc
      721 acttcagcgc cgggatcggg ccgtgcgaaa tcgtcagcgc gtcgcggccg attgcctcca
      781 ccttgccttc gccttcgtag cgcggcgcag cggcgacctg gcttgccggc gcatcgttca
      841 gccgcgcctc caacccgcgc aggctggcct cggaatcgat caggaactgc gatgacacga
      901 ccacgcgctg gcccacctgc aagccgcgct taatctcggt ctgccccccg ctttcgatgc
      961 cggcttgcac ctcgaccggc cggaagtggc cgttgtcctc ggcgagcatt accaccgtcc
     1021 gcctgccagt ctggatgacg gcgtcgctcg gcaccagcag cgccttctcc ggccgcacgt
     1081 ccatgaagtt catcgtcacg aacatgccgg gcgccaagcg gctgcccgag ttggcgagct
     1141 ccatgcgcgc cttgagcgtg cgcgtggcgg cgttcacctc gggcaggatc gcctgcaccc
     1201 ggccgtcgaa gctcatcccc ggcacagcgg ggctcttcgc ctgcaccttt gcgccggggc
     1261 gcagcaaagc cgcctggctc tcgggcacct ccgcgttggc ccagacggtg cccaggccgt
     1321 tgatgcggaa caacgtggca cccgacatca ccgtcatgcc ttcgcgcgcc atcagctcga
     1381 ccaccacgcc gccgatgggc gcggtcagcg tgatgcgtgg ctgcgtcttg ccgctgctct
     1441 cgaccaggcg gatctgctcc tcgctcatgc ccacctggcg catgcgctgg cgagcgccgt
     1501 ccagcagcgg cgcgagatcg ccgccctgca tgcggcgcac cgacaggaac tcttcctgcg
     1561 cggcgatcca gtccggcacg tacagctcgg ccagcggctg acccttgcgc accgggtcca
     1621 aggtcgcgcg gacgtaaagc cgctcgacat aggctgtggc gcgtgcctgc acgatcacct
     1681 ggtcgcgctc gttgaaggcg atgcctccga ctgcggagac ctgcggcgac agcgttccct
     1741 cggtaacctc tgccgtccga agcccgaggt tctgctggat gcgggggctg acggtgatct
     1801 tgccctcgtc accacccgcg ccttcgtaca ccggcaccag catcatgtcc atgaagggcg
     1861 acttcgacgg aacgtcaaac ttgttccccg gcaccatcgg gtcgtggtaa tagaggatct
     1921 tgcggccggt catggggtcc acgtccccgg ccttgagtcc ggccttcatg tgccggcgcg
     1981 tcgcttcttc gccttcagcg gtgctttgcg gaaggcggcc ctgcgcggcc gcgggggcgg
     2041 caggctctgc tgtagtagca gtgaccggcg cgtcctgcat gccacgatgc atgccgagtc
     2101 ggtagaggcc gtagccagac gcggcgacga tcacgacagc cgcgacggcg acagcgatgg
     2161 acgttgtttt catcgagggt tctccgagga gtcatgcgct gcaggcacga gatagttcag
     2221 ttgcgcccag aggcgagcgg tctccatctc cagcttcaac tggtccatgc gggtgtcgat
     2281 ctcggcgcga cgggcgtcca ggaccgccgc caagggcgtt ccgccaccgc ggtaagcggc
     2341 cagggccgcc tgcgtgcgtt gcacggccaa gggcagcaac tcggcttcgt agcgctgcag
     2401 gcgctgtcgg ttgctgcccc actgctggag ctgcgcgcgg gcctcggcca cg
//
LOCUS       DASUCY010000483         2087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_813971, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000483 DASUCY010000000
VERSION     DASUCY010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813971"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VFV25_09575"
     CDS             complement(<1..318)
                     /locus_tag="VFV25_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEY1393615.1"
                     /translation="MEAEVEPQQQGVPIEPGTVIDGFVLGEPLHKGGMAQLWSVSHPA
                     YELPMLMKLPRLLGGEDPSAIVGFEVEQMILPRLRGPHVPRYIARGDLTRQPYLVMER
                     IPGD"
     gene            complement(318..1061)
                     /locus_tag="VFV25_09580"
     CDS             complement(318..1061)
                     /locus_tag="VFV25_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEY1393616.1"
                     /translation="MKLAILTDLHGNREAVQACMDQAAALGAQRFVFIGDYVGYGADP
                     AWVVETVREQVARGAIAVQGNHDLAAVQGPPPSMEPAATASALWTHEQLNASQRGFLA
                     ALPLTAKLGECLFVHANAVAPNEWGYVRSRADAMHSLEATDQRLSFCGHVHEPRLYHL
                     SSTAKLLDFEPVPGVAVPLLPTRRWLVLPGSAGQPRDGNPAVCWALFDTETLDLSFQR
                     VPYDHDAAAAKVIAAGLPPALAERLRTGI"
     gene            complement(1088..2020)
                     /gene="argF"
                     /locus_tag="VFV25_09585"
     CDS             complement(1088..2020)
                     /gene="argF"
                     /locus_tag="VFV25_09585"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEY1393617.1"
                     /translation="MKPGMSLIKHYLQFKDFRAEEYAYLFERAGIIKKRFKNYEKYQP
                     LVDRTLAMIFEKASTRTRVSFEAGMYQMGGSVVHLTTGDSQLGRAEPVEDSARVISRM
                     VDIVMIRTFEQRKLEGFAAHSRVPVINGLTNEYHPCQILADLFTYIEHRGAIQGKVVA
                     WVGDGNNMAHTWLQAAEILGFTVHVSTPSGYEFDPKVAGVSNTGCYRVFNNPMDACRG
                     AHLVTTDVWTSMGYEAENEARRKAFAEWCVDAEMMGVARKDALFMHCLPAHRGEEVAA
                     DVIDGPQSAVWDEAENRMHVQKALMEYLLLGRIG"
BASE COUNT          316 a          710 c          712 g          349 t
ORIGIN      
        1 gtcgcctggg atgcgctcca tcaccaggta gggctggcgc gtgaggtcgc cgcgcgcaat
       61 gtagcgcggc acatgaggcc cgcgcagccg cggcaggatc atctgctcga cctcgaagcc
      121 gacgatggcc gagggatcct caccgcccag caggcgcggc agcttcatca gcatcggcag
      181 ctcgtaggcc gggtggctca cgctccaaag ctgggccatg ccgcccttgt gcaagggctc
      241 gcccagcacg aagccgtcga tcacggtgcc gggctcgata ggcacgccct gctgctgcgg
      301 ctcgacctcg gcctccatca gatgcccgtg cgcaggcgtt cggccagggc cggcggcagg
      361 ccggcggcga tcacttttgc cgccgccgcg tcgtggtcgt agggcacgcg ctgaaagctc
      421 aggtccagcg tctcggtgtc gaacagcgcc cagcacacgg ccgggttgcc gtcgcgcggc
      481 tggccggccg agcccggcag caccagccag cgccgtgtcg gcaggagcgg cacggcgaca
      541 cccggcaccg gctcgaagtc cagcagcttg gcggtggacg acaggtgata caggcgcggc
      601 tcgtgcacat ggccgcagaa gctcaggcgc tgatccgtgg cctccaggct gtgcatcgcg
      661 tcggcgcggc tgcgcacata gccccattcg ttcggggcca cggcattggc atgcacgaac
      721 aggcattcgc ccagcttggc agtcagcggc aaggcggcca ggaagccgcg ctgggaggca
      781 ttcagctgct catgggtcca cagcgccgag gccgtcgcgg ccggctccat gctcggcggc
      841 ggaccctgca ccgccgccag atcgtggttg ccctgcacgg cgatggcgcc gcgcgccact
      901 tgctcgcgca ccgtctcgac cacccaggcc gggtcggcgc cgtagccgac atagtcgccg
      961 atgaagacga agcgctgggc gcccagcgcg gcggcctggt ccatgcaggc ctgcacggcc
     1021 tcgcggttgc cgtgcagatc ggtgaggatg gcgagcttca tcgcagcgcc tcgtccggca
     1081 aggctcgtca gccgatgcgc ccgagcagca ggtattccat caaggccttt tggacgtgca
     1141 tgcggttctc tgcttcatcc cacaccgcgg attgcgggcc gtcaatgacg tcggccgcga
     1201 cctcctcgcc gcggtgcgcc ggcaggcaat gcatgaagag ggcgtccttg cgcgcgacgc
     1261 ccatcatctc ggcgtccacg caccattcgg cgaaggcctt gcgccgggcc tcgttctcgg
     1321 cctcgtagcc catgctggtc cacacgtcgg tggtgaccag gtgggcaccg cggcaggcgt
     1381 ccatcgggtt gttgaagacc ctgtagcagc cggtgttgct gacacccgcg accttggggt
     1441 cgaactcgta gccgctgggc gtgctcacgt gcaccgtgaa gcccaggatc tcggcggcct
     1501 gcagccaggt gtgggccatg ttgttgccgt cgcccaccca cgccaccacc ttgccctgga
     1561 tggctccgcg atgctctatg taggtgaaca ggtcggccag gatctggcag gggtggtact
     1621 cgttggtcag gccgttgatc accggcacgc gcgaatgggc agcgaagccc tcgagcttgc
     1681 gctgctcgaa ggtgcggatc atcacgatgt cgaccatgcg gctgatgacg cgcgccgagt
     1741 cttccaccgg ttcggcgcgg ccgagctggc tgtcaccggt ggtcaggtgc accaccgagc
     1801 cgcccatctg gtacatgccg gcctcgaagc tcacgcgggt gcgcgtcgag gccttctcga
     1861 agatcatcgc cagcgtgcgg tccaccagcg gctggtattt ctcatagttc ttgaagcgct
     1921 tcttgatgat gccggcgcgc tcgaacaggt aggcgtattc ctcggcgcgg aagtccttga
     1981 actgcaggta gtgcttgatg aggctcatgc cgggcttcat ggcgtcgccg tctcgctcag
     2041 gaacttcctg accagcggca cgaggatcga aacgatctgc cgcgcct
//
LOCUS       DASUCY010000484         5634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_155683, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000484 DASUCY010000000
VERSION     DASUCY010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 5634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5634
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155683"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /locus_tag="VFV25_09590"
     CDS             <1..1143
                     /locus_tag="VFV25_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEY1393618.1"
                     /translation="LGVAALIIVLSVMNGFQKEVRDRMLSVIAHVEIFDAQGGALPDW
                     QATAVAAKRNPQVQGAAPFIASQVLIARGDDMHGAMVRGISPTDEPNVTPIAAPLKDT
                     VLARLTPGSWGVVLGIELARQLGVHEGDEVTLVAPNGQVTPAGVVPRLKQMTVVGTFD
                     SGHYEYDSSLALIDIDDAARLFHVEGPTGVQLRLKDQQAAREVGSELSTALGPGVVVR
                     DWTRTNSQWFSAVQLEKRMMFIILTLIVAVAAFNLVSTLVMTVTDKRADIAILRTLGA
                     SPRSIMGIFMVQGAASGVIGTLAGLLLGLLVATNIDVIVPALERLFNASFLPGSIYLI
                     TRMPSDPQSGDIVPIAVISLLLAFVATLYPSWRASRVQPAEALRYE"
     gene            1136..1822
                     /locus_tag="VFV25_09595"
     CDS             1136..1822
                     /locus_tag="VFV25_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY1393619.1"
                     /translation="MSEVVLQGQGLTRRFHEGGRNGLDVTVLHGVDLHVARGETLAVV
                     GASGSGKSTLLHLLGGLDAPTSGAVQLMGRDFARMDAAGQGEWRNRHLGFIYQFHHLL
                     PEFSALDNVAMPLMIRRQTREQAREQAAQMLHDVGLGGRLRHRPAELSGGERQRVAVA
                     RALVAGPSCVLADEPTGNLDRDTAQAVFELMLKLAQERGTAFVVVTHDPTLAARCSRT
                     LRLVAGVLQP"
     gene            complement(1876..3108)
                     /locus_tag="VFV25_09600"
     CDS             complement(1876..3108)
                     /locus_tag="VFV25_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HEY1393620.1"
                     /translation="MIETTVAIFVLVYVGMVLGGFPFLQLDRTGVALLGAIALIGIDA
                     LDLEEAARAIHLPTIILLFAFMVVSAQMRLGGFYDWVTRRIAELPLRPPLLLGALIFA
                     VAALAAVFSNDVVCLAAAPVLVDACRSRRLHALPFLLALACASNIGSAATLIGNPQNM
                     LIGETLHLSFAGYLLEAAAPVLLGLIATWGLILWCSSGRLVCEPAPAEPSRQDAPVLD
                     RWQTAKGLAVAFVIVLCFLLTPWPREVVALTGAGFLLMSRKLHSRSMLGLVDWELLVL
                     FMGLFVVNQALHKTGLPARLMADLDASGVPLHHPAPLFAATFALSNLVSNVPAVMLLL
                     PGAQHPLAGPELALVSTLSGNLLIVGSIANIIVVDAAARQGVRITWRDHLRVGLPVTL
                     ATLAIAAGFLALRLRAWA"
     gene            complement(3235..4257)
                     /locus_tag="VFV25_09605"
     CDS             complement(3235..4257)
                     /locus_tag="VFV25_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEY1393621.1"
                     /translation="MTWLWPGLLWWLVLLPLLVLLYVLLQRRRRRHTLRYASLSLVKD
                     ALGRGSGWRRHVPPFVMLLALAALLLATARPAAVVTLPSQQETIILAMDVSGSMRATD
                     VEPSRLVASQNAAKAFIATLPRHVRVGVVAFAGTAAVVQAPTLSREDVVAAIDRFQLQ
                     RGTAIGSGIVLALAALLPDAGIDLAQITGDREPGLGDTEPRQAAPVAPGSYDTGAIIL
                     LTDGQRTTGPDPMDAAKLAANHGVRVYTVGFGTVNGEVIGFEGWSMRVRLDEEALKAI
                     ATVTHAEYFYAGSARDLQRVYDTLSSRLVFEKKETEITALFAALGAALALLAAGLSLW
                     WFGRVM"
     gene            complement(4254..5231)
                     /locus_tag="VFV25_09610"
     CDS             complement(4254..5231)
                     /locus_tag="VFV25_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEY1393622.1"
                     /translation="MDSKPQLAPEAAEATLRRLEWTVLRRLDGALQGDYRTLFRGAGL
                     DLADLREYQHYDEVRHIDWNVTARLGTPYVREYHEDRELSAWFLLDLSPSVDFGSQRV
                     RKLSVAADFTALLARLLTRRGNRVGALLFDAKLLAVMPPLGGRRQVLHLLDRIAKHQS
                     TDATGSVGSITKLQELLQAAQRLIKRRSLLFLVSDFISQPGWEASLSRLALRHDVVAV
                     KLHDPMELALPDLGMVWLQDAETGEQLLVDTHDRRLRRRFEALAGKRQDELRAALGHA
                     GVDALELSTDDDLLSATLRFAELRRQRSRLATGGGLPLHLAPQRKEGRS"
     gene            complement(5203..>5634)
                     /locus_tag="VFV25_09615"
     CDS             complement(5203..>5634)
                     /locus_tag="VFV25_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEY1393623.1"
                     /translation="AAVADTEQLAALQRQCREVYVDPSLIQYAVRLVGASREPARHGL
                     GDISKYISFGASPRASIHLIEAARALAFLRGRNYALPEDVSDLVPDVLRHRLVLSYEA
                     LTDELSADTLIQRIVVKTPAPDKPLAHRSEAGHGQQAAAGA"
BASE COUNT          905 a         1912 c         2006 g          811 t
ORIGIN      
        1 ctgggcgtgg ccgcgctcat catcgtgctg tcggtgatga acggcttcca gaaggaggtg
       61 cgcgaccgca tgctgtcggt gatcgcccat gtcgagatct tcgacgccca gggcggagca
      121 ttgcccgact ggcaggcgac cgcggtggcg gccaagcgca atccgcaggt gcagggcgca
      181 gcgcctttca tcgcttcgca ggtgctgatc gcacgcggcg acgacatgca tggagcgatg
      241 gtgcgcggca tctcgccgac cgacgagccc aacgtgacgc cgatcgccgc cccgctcaag
      301 gacacggtgc tggcgcggct cacgcccggc tcctggggcg tggtgctggg catcgagctg
      361 gcgcgccagc tcggcgtgca cgagggcgac gaggtgacgc tggttgcgcc caacggccag
      421 gtgacgccgg ccggcgtggt gccgcggctc aagcagatga cagtggtggg caccttcgat
      481 tccggccact acgaatacga cagcagcctg gcgctgatcg acatcgacga tgcggcgcgg
      541 ctcttccatg tcgaggggcc gaccggcgtg cagctcaggc tcaaggatca gcaggccgcg
      601 cgcgaggtgg gcagcgagct ctccaccgcg ctcggccccg gcgtggtggt gcgcgactgg
      661 acgcgcacca acagccagtg gttctcggcc gtgcagctcg agaagcgcat gatgttcatc
      721 atcctcacgc tgatcgtcgc ggtggcggcc ttcaacctcg tgtcgacgct ggtcatgacc
      781 gtgaccgaca agcgcgccga cattgccatc ctgcgcaccc tgggcgccag cccgcgcagc
      841 atcatgggca tcttcatggt gcaaggcgcc gcctcgggcg tgatcggcac gctggccggg
      901 ctgctgctgg gcctgctcgt ggccaccaac atcgacgtca tcgtgccggc gctggagcgg
      961 ctcttcaacg ccagcttcct gcccggcagc atctatctca tcacgcgcat gcccagcgac
     1021 ccgcagtcgg gcgacatcgt gccgatcgcg gtcatctcgc tgctgctggc ttttgtcgcg
     1081 acgctctatc cgagctggcg cgctagtcgc gtacagccgg cggaggcctt gcgctatgag
     1141 tgaagttgtc ctccaaggcc agggcctgac gcggcgcttc cacgaaggcg ggcgcaacgg
     1201 cctggacgtg acggtcctgc atggcgtcga tcttcatgtg gcgcgcggcg agacgctcgc
     1261 cgtcgtcggt gcctcgggct cgggcaagag cacgctgctg cacctgctgg gtgggctgga
     1321 tgcgccgacc tcgggcgccg tacagctgat gggccgggac ttcgcgcgca tggacgcggc
     1381 cggacagggc gaatggcgca accggcatct gggcttcatc taccagttcc atcacctgct
     1441 gcccgagttc agcgcgctcg acaacgtggc gatgccgctg atgattcgtc gccagacgcg
     1501 cgagcaggcg cgcgagcagg cggcgcaaat gctgcacgac gtgggcctgg gcggccgcct
     1561 gcggcatcgc ccggccgagc tctccggcgg cgagcgtcag cgcgtggcgg tggcgcgcgc
     1621 gctggtggcc ggcccgagct gcgtgctggc cgacgagccc accggcaacc tcgaccgcga
     1681 cacggcgcaa gccgtgttcg agctgatgct caagctcgcg caggagcgcg gcaccgcctt
     1741 cgtcgtcgtg acgcacgacc cgactctggc ggcgcgctgc agccgcacgt tgcggctggt
     1801 ggcgggagtg ctgcagccct gatcgaggtt ggccgcggga gccgtgcagc ttggcgctct
     1861 cgatttgcgg cgagatcaag cccaggcgcg cagcctcagc gccagaaatc ccgcggcgat
     1921 cgcgagcgtg gccagcgtga ccggaaggcc gacgcggagg tggtcgcgcc aggtgatgcg
     1981 aaccccttgc cgcgccgccg cgtcgacaac gatgatgttg gcgatgctgc ccacaatgag
     2041 caggttgccc gacagggtgc tcaccagcgc cagctcgggg ccggccagcg ggtgctgggc
     2101 gccgggcagc aacagcatga cggcgggaac gttcgacacg aggttcgaca gggcgaaggt
     2161 cgcggcgaag agcggcgcag gatgatgcag cggcacgccg gaagcatcga ggtcggccat
     2221 cagccgggcc ggcaggccgg tcttgtgcag ggcttggttg acgacgaaca gccccatgaa
     2281 gagcaccaga agctcccagt ccaccaggcc gagcatgctt ctcgaatgaa gcttgcggct
     2341 catcaacagg aatcccgccc ccgtcagggc gaccacctcg cgcggccacg gcgtgagcag
     2401 gaagcacagc acgatgacaa atgcaaccgc cagtcccttg gcggtttgcc agcggtcgag
     2461 cacgggcgcg tcctggcggg acggctcggc cggggccggc tcgcaaacca ggcggccgct
     2521 gctgcaccac aggatcagcc cccaagtggc gatcagcccc agcagaaccg gcgccgccgc
     2581 ctccaggagg tagccggcga aggacaggtg cagcgtctcg ccgatcaaca tgttctgtgg
     2641 attgccgatc agcgtggcgg ccgagccgat gttcgaggcg caggccagcg ccagcaggaa
     2701 gggcagagcg tgcagccgcc ggctgcggca ggcatcgacg aggaccggcg ctgcggcgag
     2761 gcagaccacg tcgttgctga acaccgcggc cagggccgcg acggcgaaga tcaaggcccc
     2821 cagcagcaag ggcgggcgaa gcggcagctc tgcaatccgg cgcgtcaccc agtcatagaa
     2881 gccgcccaga cgcatctggg ccgataccac catgaaggcg aatagcagga tgatcgtggg
     2941 caggtggatc gctcgcgccg cttcttcaag atccagggcg tcgatgccga tcaaggcgat
     3001 cgcccccagc agcgccacgc cggtgcggtc gagctggaga aacggaaagc cgcccaggac
     3061 catgcccaca tacacaagga cgaagatggc gacggtggtt tcgatcattt cttgtctcgt
     3121 ggcggttctt ccgatcggcg atgcgatcgt gaggctttag ccctgcaggc cccgggggaa
     3181 tgcggcttcc actacggaat ctatcaagcc ggcttgtcaa gccgactcta gccgctacat
     3241 cacccgcccg aaccaccaca gagacagccc cgccgccagc agcgccagcg cggcgcccag
     3301 ggcggcgaag agcgccgtga tctcggtttc cttcttctcg aatacgaggc gtgagctgag
     3361 cgtgtcgtag acgcgctgca ggtccctggc gctgccggcg tagaagtact cggcatgggt
     3421 gacggtggcg atcgccttga gcgcctcctc gtccagccgt acgcgcatgg accagccctc
     3481 gaagccgatc acctcgccat tgaccgtgcc gaagcccacg gtgtagacgc gcacgccgtg
     3541 gttggccgcg agcttggcgg cgtccatggg gtcggggccg gtggtgcgct ggccgtcggt
     3601 gagcaggatg atggcgccgg tgtcgtagga gcccggcgcg acgggcgctg cctgcctggg
     3661 ctccgtatcg cccaagccgg gttcacggtc gccggtgatc tgcgcgagat cgatgccggc
     3721 atcgggcagc agggccgcca gcgccagcac gatgccgctg ccaatggccg tgccgcgctg
     3781 cagctggaag cggtcgatcg cggccaccac gtcttcgcgg ctcagcgtgg gcgcctgcac
     3841 gaccgcggcc gtgccggcga aggcgaccac acccaccctc acatggcgcg gcagcgtcgc
     3901 gatgaaggcc ttggccgcgt tctgcgaggc cacgaggcgg ctgggctcga catcggtggc
     3961 gcgcatgctg cccgacacgt ccatcgcgag gatgatggtc tcctgctgcg agggcagcgt
     4021 gaccaccgcg gccggccgcg ccgtggcgag cagcagggcg gcgagcgcca gcagcatcac
     4081 aaagggcggc acatggcggc gccagccgct gccgcgcccc agcgcatcct tcacgaggct
     4141 caggctggca tagcgcagcg tgtggcgccg gcggcggcgt tgcagcagca cgtagagcag
     4201 caccaagagc ggtagcaata ccagccacca cagcaagccg ggccacaacc acgtcatgac
     4261 cggccctcct tgcgctgcgg cgccaggtgc agcggcagcc cgccgcccgt ggccaggcgg
     4321 ctgcgctggc ggcgcagctc ggcgaagcgc agggtcgcgc tcagcagatc gtcgtcggtc
     4381 gacagctcca gcgcgtcgac gccggcgtgg ccgagcgcgg cgcgcagctc gtcctgccgc
     4441 ttgcctgcca gcgcctcgaa gcggcggcgc aggcggcggt cgtgcgtgtc caccagcagc
     4501 tgctcgccgg tctcggcgtc ctgcagccag accatgccca gatcgggcag ggccagctcc
     4561 atggggtcgt gcagcttgac ggccaccacg tcatggcgca gggccaggcg cgagagcgac
     4621 gcttcccaac ccggttggct gatgaagtca gagacgagga agagcagcga gcgccgcttg
     4681 atcagccgct gcgccgcctg cagcagttcc tgcagcttgg tgatcgagcc aacggagccc
     4741 gtcgcatcgg tggactggtg tttggcgatg cggtccagca gatgcagcac ctggcggcgc
     4801 ccgccgaggg gcggcatcac ggccagcagc ttagcgtcga acagcagggc gccgacgcgg
     4861 ttgccgcgcc gcgtcagcag gcgcgccagc agggccgtga aatcggcggc caccgagagc
     4921 ttgcgcacgc gctgcgagcc gaagtccacc gaagggctca gatccagcag gaaccaggcg
     4981 ctcagctcgc ggtcctcgtg gtattcgcgc acatagggtg tacccagccg cgcggtgacg
     5041 ttccagtcga tgtggcgcac ctcgtcgtag tgctggtact cgcgcaggtc ggccaggtcc
     5101 aggccggcgc cgcgaaacag cgtgcggtag tcgccctgca gcgccccgtc gaggcggcgc
     5161 agcaccgtcc actccagccg ccgcagagtg gcttcggcgg cttcaggcgc cagctgcggc
     5221 ttgctgtcca tggcccgcct cgctgcgatg ggcaagcggc ttgtcaggtg ccggcgtctt
     5281 cacgacgatg cgctggatca gcgtgtcggc gctcagctcg tcggtcagcg cctcgtagct
     5341 cagcacgagg cggtggcgca gcacatcggg caccaggtcg ctcacgtcct cgggtagcgc
     5401 atagttgcgc ccgcgcagga aggccagggc gcgcgccgcc tcgatcagat ggatgctggc
     5461 gcgcgggctg gcgccgaagc tgatgtactt cgaaatgtcg cccaggccat ggcgcgccgg
     5521 ctcgcgactc gccccgacca gcctcaccgc gtactggatc agcgaagggt cgacatagac
     5581 ctcgcggcat tgccgctgca gggccgccag ttgctcggtg tcggcaacgg ctgc
//
LOCUS       DASUCY010000485         2036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_412509, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000485 DASUCY010000000
VERSION     DASUCY010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412509"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="VFV25_09620"
     CDS             complement(<1..1041)
                     /locus_tag="VFV25_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HEY1393624.1"
                     /translation="MPAGKRTTTSRARPRPDVDTLLQSYSAKRNFEHTPEPSGGIAPA
                     AGGFSYVTQKHWASRLHYDFRLELDGVLLSWAVPKGPCYDPGEKRMAVHVEDHPVAYG
                     GFEGTIPRGQYGAGTVIVWDRGTWEPVGDPRAGMANGKLVFKLHGEKLAGLWELVRIA
                     KPGDRQDPWMLFKKRDAWARPLAEYDVIAALPDSVIDKPLGLVEQRQPRGGCNGAAAP
                     APAVRIASAAELPGAVKAELPEALEPQLATLTKAVPAHGEWISELKLDGYRLLARIDA
                     RGGVRLITRNGNDWTPRMKTLRDAVAALGLRSCWLDGEIAILKNDGTPDFNALQNAFD
                     SARTEAIEYFLFD"
     gene            complement(1043..1726)
                     /locus_tag="VFV25_09625"
     CDS             complement(1043..1726)
                     /locus_tag="VFV25_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEY1393625.1"
                     /translation="MEAATPQAFTLPVSDQQSVSALLLAPANARACYVLAHGAGAGMT
                     HTFMEAVAQGLAARDIATLRYQFPYMERGSRRPDAPPLAHATVRAAVAEAARRLPGLP
                     LLAGGKSFGARMSSQAQAVQPLPGVVALAFLGFPLHPAGQPSQERAAHLQQVRCPMWF
                     WQGTRDELADMTLLQPVIAGLGSRATLEIAPEADHSFHVPARSGRKDAEVLSSGLEAL
                     VHWVDRLLS"
     gene            complement(1806..>2036)
                     /locus_tag="VFV25_09630"
     CDS             complement(1806..>2036)
                     /locus_tag="VFV25_09630"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEY1393626.1"
                     /translation="LTCDPVGGLVQIPCIERNAIASVKAINAARMALRGDGTHHVSLD
                     KVIKTMWETGADMKTKYKETARGGLAVNIIEC"
BASE COUNT          298 a          694 c          706 g          338 t
ORIGIN      
        1 atcgaacagg aagtattcga tcgcctcggt gcgcgcactg tcgaaggcgt tctgcagcgc
       61 gttgaagtcg ggcgtgccgt cgttcttcag gatcgcgatc tcgccgtcga gccagcatga
      121 gcgcaggccg agcgccgcta cggcgtcgcg cagggtcttc atcctcgggg tccagtcgtt
      181 gccattgcgg gtgatgaggc gcacgccgcc gcgcgcgtcg atgcgcgcga gcagccggta
      241 gccgtcgagc ttgagctcgg agatccattc gccgtgtgca ggcaccgcct tggtcaacgt
      301 ggcgagctgg ggctccagcg cctcgggcaa ctcggccttc accgcgcccg gcagctcggc
      361 cgccgacgct atgcgcacgg ccggtgcggg cgcggccgcg ccgttgcaac cgccgcgcgg
      421 ttggcgctgc tccaccaggc ccagcggctt gtcgatgacg ctgtcgggca gtgcggcgat
      481 cacgtcgtat tcagccagcg ggcgcgccca ggcgtcgcgc ttcttgaaca gcatccacgg
      541 gtcctgccga tcgccgggct tggcgatgcg caccagctcc cacaggcccg cgagcttttc
      601 gccgtgcagc ttgaacacaa gcttgccatt ggccatgcct gcgcgcgggt cgcccacagg
      661 ctcccaggtg ccgcggtccc agacgatgac cgtgcccgcg ccgtactgcc cgcgcgggat
      721 ggtgccttcg aagccgccat aggccacggg gtggtcttcc acgtgcacgg ccatgcgctt
      781 ctcgccgggg tcgtagcagg ggcccttggg caccgcccag ctcagcagca caccgtcgag
      841 ctcgaggcga aagtcgtagt gcagccgcga agcccagtgc ttctgggtca catagctgaa
      901 gccgccggcc gcaggagcaa tcccgcccga tggttcgggc gtgtgctcga aattccgctt
      961 cgcgctgtag ctttgcagca gcgtgtcgac gtcggggcgg gggcgcgccc tgctcgttgt
     1021 cgtgcgcttg ccagcgggca tatcaactca ggagcctgtc gacccagtgc accagcgcct
     1081 cgagcccgct gctcaacacc tcggcatcct tgcggccgct gcgcgccggc acgtggaagg
     1141 agtggtcggc ctccggcgcg atctccagcg tggcgcgcga acccaggcct gcgatgaccg
     1201 gttgcagcag ggtcatgtcc gccagttcgt cgcgcgtgcc ttgccagaac cacatcgggc
     1261 agcgcacctg ctgaaggtgc gcggcgcgct cctgcgaggg ctggccggcc gggtgcagcg
     1321 gaaagccgag gaaggccaaa gcaacgacgc cgggcagcgg ctgcactgcc tgggcctgcg
     1381 agctcatgcg cgcgccgaaa gacttgccgc cggccagcaa cggcagcccc ggcaggcggc
     1441 gtgccgcctc ggccaccgct gcgcgcaccg tcgcatgagc gaggggtggc gcatccggcc
     1501 gcctggagcc gcgctccata taggggaact ggtagcgcag cgtggcgatg tcgcgagcgg
     1561 caaggccttg cgccacggcc tccatgaagg tgtgagtcat gccggcgcca gcgccatggg
     1621 ccagcacata gcaggcgcgc gcattcgccg gcgccagcag cagggccgag acgctctgct
     1681 ggtcgcttac gggcagcgta aaggcttgcg gggtggctgc ttccatcgag atcgcgtccc
     1741 tttaaacgca acccacgcat tctgctcagc tttgcgttcc catgcgagga cgcgaggtgg
     1801 caggctcagc actcgatgat attgacggcc aagccgccgc gggccgtttc cttgtacttg
     1861 gtcttcatgt cggctccggt ttcccacatg gtcttgatga ccttgtcgag gctgacgtga
     1921 tgggtgccgt cgccacgtag cgccatgcgt gcggcattga tggccttcac cgaggcgatg
     1981 gcgttgcgct cgatgcaggg gatctgcacc aggccgccca ccggatcgca ggtcag
//
LOCUS       DASUCY010000486         3868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_284022, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000486 DASUCY010000000
VERSION     DASUCY010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284022"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VFV25_09635"
     CDS             <1..407
                     /locus_tag="VFV25_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY1393627.1"
                     /translation="GVFLVTVWFAPPSLVEPWWLLVFALLGAALMGALGLVAGLWAEK
                     FDQMAAFQNFLIMPMTFLAGVFYSVHSLPGIWQAMSHLNPFFYMIDGFRRGFFGVSDV
                     SPWLSLVVVGVSFLIVAGLALHLLKTGYKLRH"
     gene            432..710
                     /locus_tag="VFV25_09640"
     CDS             432..710
                     /locus_tag="VFV25_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HEY1393628.1"
                     /translation="MSEPTPVEVERYIAQGLACEHLQVEGDGRHFFATIVSSDFEGKA
                     RVARHQRVYQALGDRMREQIHALSMKTLTPAEFAAQGASAPAATAHHH"
     gene            810..2087
                     /gene="murA"
                     /locus_tag="VFV25_09645"
     CDS             810..2087
                     /gene="murA"
                     /locus_tag="VFV25_09645"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEY1393629.1"
                     /translation="MDKLLIRGGRRLQGEVVISGAKNAALPELCAALLTADPVTLANV
                     PHLQDVATTLKLLRSMGAEAERSQSQPDQVAINAGPLSSPEAPYDLVKTMRASILVLG
                     PLLARFGEATVSLPGGCAIGSRPVDQHIKGLQAMGADIQVEHGYIIAKAPKSKGGLKG
                     ARITTDMVTVTGTENLLMAAALAEGETVLENAAQEPEIPDLAELLIKMGARIEGQGTS
                     RIRIQGVERLHAPREPHRIIPDRIETGTFLCAVAAAGGDVLLKHADASHLDAVIDKLR
                     EAGAGIEAGAGWIRVKSDRRLKSVSFRTSEYPAFPTDMQAQFMALDCVAQGSARISET
                     IFENRFMHVNELVRLGAKIEVDGHTAVVTGVERLSGATVMATDLRASASLVIAGLVAS
                     GETMIDRIYHLDRGYDRMEAKLRGIGADIERVK"
     gene            2084..2719
                     /gene="hisG"
                     /locus_tag="VFV25_09650"
     CDS             2084..2719
                     /gene="hisG"
                     /locus_tag="VFV25_09650"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEY1393630.1"
                     /translation="MITLALSKGRIFEETLPLLKAAGIEVTEDPETSRKLILPTSRAD
                     VRVVLVRATDVPTYVQYGGADLGVAGKDVLLEHGGDGLYQPLDLRIAKCRMSVATRAD
                     FDYAAAVKQGSRIRVATKYVRVAREHFADKGVHVDLIKLYGSMELAPLTGLADAIVDL
                     VSTGGTLKANKLVEVEKIMDISSRLVVNQAALKLKREAIRRLIDAFSTAIR"
     gene            2728..>3868
                     /gene="hisD"
                     /locus_tag="VFV25_09655"
     CDS             2728..>3868
                     /gene="hisD"
                     /locus_tag="VFV25_09655"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEY1393631.1"
                     /translation="MAAVSIRQLQTSAPGFEAEFQRVLHWSAEQDHEIEQRVAAILAD
                     VKASGDAAVLEYTARFDGVEAASVAALELTRDELKAAFDAITPAQRRALEAAAARVRS
                     YHERQLEACGRSWSYRDADGTLLGQKVTPLDRVGIYVPGGKAAYPSSVLMNAIPAQVA
                     GVAEIVMVVPTPRGEKNPLVLAAAYVAGVHRAFTVGGAQAVAALAYGTATIPRVDKIT
                     GPGNAYVASAKKHVFGQVGIDMIAGPSEILVLADGSTPADWVAMDLFSQAEHDELAQS
                     ILLCPDADYIARVKAEIERLLPGMPRRDVIRASLEGRGALIQTRSMEEACEISNRIAP
                     EHLEVSSREPQRWEPLLRHAGAIFLGAYTSESLGDYCAGPNHVLPT"
BASE COUNT          652 a         1311 c         1295 g          610 t
ORIGIN      
        1 tgggggtctt cctcgtgacc gtgtggttcg cgccgcccag tctggtcgag ccctggtggc
       61 tcctcgtgtt cgccctgctc ggcgcggccc tgatgggggc gctcggcctg gtggcggggc
      121 tgtgggccga gaagttcgat cagatggcgg ccttccagaa ttttctgatc atgccgatga
      181 cctttttggc cggcgtgttc tattcggtgc actcgctgcc cgggatctgg caggccatga
      241 gccacctcaa ccccttcttc tacatgatcg acggcttcag gcgcggcttc ttcggcgtca
      301 gcgatgtgtc gccctggctg agcctcgtcg tggtcggcgt gagcttcctg atcgtggccg
      361 gcctggcctt gcatctgctc aagaccggtt acaagctacg gcattgagct ggcagctaaa
      421 atcgccccat catgtccgag ccgacccccg tcgaagtcga gcgctatatc gcgcagggtc
      481 tggcctgcga gcacctgcag gtcgagggcg acggccgtca tttcttcgcg accatcgtgt
      541 cgagcgactt cgagggcaag gcccgcgtgg cgcgccacca gcgcgtctac caggccctgg
      601 gcgatagaat gcgtgagcaa atccacgccc tgtcgatgaa gacgctgacg ccggccgaat
      661 ttgcggccca aggggcttcg gctcccgccg ccaccgccca ccaccattga cacccaagcc
      721 gcagcgcacg cgcggcttgc ggaccatctc gcgcgaggtg gggccaaacg caagcgcatg
      781 gatgctgggg cacagaacac caaacatcca tggacaaact cctgatacgc ggcggccgcc
      841 ggctccaagg cgaagtcgtg atctccggcg ccaagaacgc ggctctgcct gagctgtgcg
      901 cggccttgct caccgccgat ccggtgacgc tcgccaacgt gccacacctg caggacgtgg
      961 ccaccacgct caagttgctg cgcagcatgg gcgccgaggc cgagcgctcg cagagccagc
     1021 ccgatcaggt ggcgatcaat gccggcccgc tcagctcgcc cgaggcgccc tacgacctcg
     1081 tgaagaccat gcgtgcctcc atcctcgtgc tggggccgct gctggcgcgc ttcggcgagg
     1141 cgacggtctc gctacccggc ggctgcgcga tcggctctcg cccggtggat cagcacatca
     1201 agggcctgca ggccatgggc gccgacatcc aggtcgagca cggctacatc atcgcgaagg
     1261 ccccgaagtc caagggcggg ctgaagggtg ccaggatcac gaccgacatg gtcaccgtca
     1321 ccggcaccga gaacctgctg atggcggccg cgttggccga gggcgagacg gtgctcgaga
     1381 acgcggccca ggagcccgag atccccgacc tggccgagct gctgatcaag atgggcgcgc
     1441 gcatcgaggg ccagggcacg agccggatac gcatccaggg cgtcgagcgc ttgcacgcgc
     1501 ccagggagcc gcatcgcatc atccccgacc gcatcgagac cggcaccttc ctgtgcgcgg
     1561 tggccgcggc cgggggcgac gtgctgctca agcacgccga cgccagccac ctcgacgccg
     1621 tgatcgacaa gctgcgcgag gccggcgccg gcatcgaggc cggggccggc tggatccgcg
     1681 tgaaatcaga ccggcggctg aagagcgtga gctttcgcac cagcgaatac ccggccttcc
     1741 cgaccgacat gcaggcccag ttcatggcgc tggattgcgt ggcccagggc agcgcccgca
     1801 tcagcgagac catcttcgag aaccgcttca tgcacgtcaa cgagctggtg cgcctgggcg
     1861 cgaagatcga ggtcgatggg cacacggcgg tcgtgaccgg agtcgaaagg ctctcgggcg
     1921 ccaccgtgat ggcgaccgac ctgcgcgcct cggccagcct ggtgattgcc ggcctggtgg
     1981 ccagcggcga gaccatgatc gatcgcatct accacctgga tcgcggctac gaccggatgg
     2041 aagccaagct gcgcggcatc ggggccgaca tagaaagagt taaatgatta cgcttgcgct
     2101 ctccaaaggt cggatcttcg aagagacctt gccgctgctg aaggcggccg gcatcgaggt
     2161 caccgaagac cccgagacct cgcgcaagct catcctgccg acctcgcgtg ccgatgtgcg
     2221 cgtggtgctg gtgcgcgcca ccgatgtgcc gacctatgtg cagtatggcg gtgctgacct
     2281 gggcgtggcc ggtaaggacg tgctgctcga gcatggtggc gacggcctgt accagccgct
     2341 ggacctgcgc atcgccaagt gccgcatgag cgtggcgacg cgcgccgact tcgactatgc
     2401 cgcggcagtg aagcagggct cgcgcatccg tgtagccacc aagtacgtgc gcgtggcgcg
     2461 cgagcatttc gccgacaagg gcgtgcatgt cgacctcatc aagctctacg gctcgatgga
     2521 gctggcgccg ctcaccgggc tggccgacgc catcgtcgac ctggtatcca ctggcggcac
     2581 gctcaaggcc aacaagctgg tcgaggtcga gaagatcatg gacatctcct cgcgcctcgt
     2641 cgtcaaccag gctgcgctca agctcaagcg cgaggccata cgccgactca tagacgcttt
     2701 ctcaactgcc atcaggtaat cgacatcatg gctgccgtct ccatacgcca actgcaaacc
     2761 agcgccccgg gcttcgaagc cgagtttcag cgcgtactgc attggtcggc ggagcaggat
     2821 catgaaatcg agcagcgcgt ggccgccatc ctggccgacg tgaaggcgag cggcgacgcc
     2881 gcggtgctgg agtacaccgc gcgcttcgat ggcgtggaag ccgcttcggt ggcggctctg
     2941 gaactcacgc gcgatgagct gaaggccgcc ttcgatgcca tcacgccggc gcagcgccgc
     3001 gcgctcgaag ccgcagcggc gcgcgtgcgc agctatcacg aacgccagct cgaggcctgc
     3061 ggccgcagct ggagctatcg cgatgccgac ggcacgctgc tcggccagaa ggtcacgccg
     3121 ctggaccgcg tggggatcta tgtgcccggc ggcaaggcgg cctatccgtc cagcgttctg
     3181 atgaatgcga tcccggcgca ggtggccggt gtcgccgaga tcgtgatggt cgttcccacg
     3241 ccaaggggtg agaagaaccc gctggtgctg gccgccgcct acgtggccgg cgtgcatcgc
     3301 gccttcacgg tgggcggcgc gcaggccgtc gccgcgctgg cctacggcac cgccaccatc
     3361 ccgcgcgtcg acaagatcac cggcccgggc aatgcctatg tagcctcggc caagaagcat
     3421 gtgttcggcc aggtcggcat cgacatgatt gccggcccca gcgagatcct ggtgctggcc
     3481 gacggctcga cgccggccga ctgggtggcg atggatctgt tcagccaagc cgagcacgac
     3541 gagctcgcgc agagcatctt gctgtgtccg gacgccgact acatcgcgcg ggtcaaggcc
     3601 gagatcgagc gcctgctgcc cggcatgccg cggcgcgacg tgatccgcgc ctcgctcgaa
     3661 ggccgcggcg cgctgatcca gacgcgcagc atggaagagg cctgcgagat cagcaaccgc
     3721 atcgcgccgg agcacctgga ggtgagctcg cgcgagccgc agcgctggga gccgctgctc
     3781 aggcatgccg gtgctatctt cctcggcgcc tacaccagcg agagcctggg cgactactgc
     3841 gccgggccca accatgtgct gcccacct
//
LOCUS       DASUCY010000487         4489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_637748, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000487 DASUCY010000000
VERSION     DASUCY010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4489
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637748"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /gene="aspS"
                     /locus_tag="VFV25_09660"
     CDS             complement(<1..966)
                     /gene="aspS"
                     /locus_tag="VFV25_09660"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEY1393632.1"
                     /translation="MRTTYCGLVSEALMGQTVTLMGWAHRRRDHGGVIFVDLRDREGL
                     VQIVCDPDRPEMFKTAEGVRNEFCLKIVGKVRPRPAGTENAGLTSGKIEVLGHELEVL
                     NASVTPPFQIDDENLSETTRLTHRVLDLRRPYMQKNLMLRYRVAMEVRKFLDAQGFID
                     IETPMLTKSTPEGARDYLVPSRVHDGMFFALPQSPQLFKQLLMVAGFDRYYQVTKCFR
                     DEDLRADRQPEFTQIDIETSFLGEEEIRAITEAMIRTVFKSVMGVELPEYPVMKYAEA
                     MHRYGSDKPDLRVKLEFTELTDVMKNVDFKVFSTPATTKGGRVAAL"
     gene            complement(1097..1768)
                     /locus_tag="VFV25_09665"
     CDS             complement(1097..1768)
                     /locus_tag="VFV25_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEY1393633.1"
                     /translation="MKKYIIAGLLVWLPLAITIWVLLWVMGLIDGVFASVVSAGQAVL
                     PESAREGLEALKRVPGLSLVVVAAALLLTGMLAANMFGQWWLRQGDRLLNHIPIVKSI
                     YSSVKQVSDTLFSSSGNAFREAVLVQYPRSGAWTIAFVTGRPGGEVANYLNAEDYLSL
                     YVPTTPNPTSGFFLMVPRADVIKLEMSVDEALKYIISMGVVAPPQVAEAAANAALADG
                     RNLGG"
     gene            complement(1806..2138)
                     /locus_tag="VFV25_09670"
     CDS             complement(1806..2138)
                     /locus_tag="VFV25_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEY1393634.1"
                     /translation="MPIYAYRCASCGHAQDVLQKISDPLLSRCPACGAETFAKQLTAP
                     GFQLKGSGWYATDFRNGGQANGSGAAATSASAATAPADSAAAPASGSSGSSTEAGAKP
                     CGGSCACH"
     gene            complement(2216..3670)
                     /locus_tag="VFV25_09675"
     CDS             complement(2216..3670)
                     /locus_tag="VFV25_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEY1393635.1"
                     /translation="MNVTLLVFVILYLVGTLAIGVWAGTRISSTSDFAIAGRSLPLAM
                     IVTTTFATWFGAETVMGIPAKFVQGGLSAIVEDPFGAGTCLILVGLFFAAKLYRMNLL
                     TIGDFYRQRFGKGVEIFCSVAIILSYLGWVAAQITALGVVFSVLSNGAISETVGMVVG
                     TIAVLVYVIIGGFLAVAWTDFIQMIVLVIGLSIIAIFAGNLAGGPSKVLALASQRQLW
                     TLMPEVSFKDVTFFVGAAVTMMLGSIPQQDVFQRVMSAKDAATARNGAAIGGVSYIFF
                     AFVPMFIVGCAVVVMGDHALELAKNDYQKLLPGFIMTHMPLVMQILFFGALLSAIKST
                     SSATLLAPSTSFVENILKNMVPGMRDRQVLLAMRITIFVFAVLVLSYAIAMKGTPIYD
                     LVSSAYQVTLVAAFVPLAAGLYWKRATTQGAILSITLGVAVWLLFLLPQLTNWGEAFP
                     AQLAGLVAALAGMVVGSLAPQLVAPSTPAAQAQA"
     gene            complement(3883..>4489)
                     /locus_tag="VFV25_09680"
     CDS             complement(3883..>4489)
                     /locus_tag="VFV25_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HEY1393636.1"
                     /translation="FIAFFRRDYKRVAELHVESGWVPRDTRVDELEGAVRAVCEPHFD
                     RPLKDISLGQVLLRLFQASRRFNVEIQPQLVLLQKTLLNVEGLGRQLDPELDLWRTAK
                     PFLERWMNEQIGWRALLTQLRNEAPRYAQLLPELPRLLHDALLARTARGLSSAPDQLQ
                     LTALLAEQRRTNRLLQGVVYGGLGFLIGLVLTLVMTRVHFF"
BASE COUNT          798 a         1428 c         1537 g          726 t
ORIGIN      
        1 cagcgcggcg acgcgcccgc ccttggtggt ggccggcgtc gagaagacct tgaagtcgac
       61 gttcttcatc acgtcggtca gctcggtgaa ctcgagcttg acgcgcaggt cgggcttgtc
      121 cgagccgtag cggtgcatgg cttcggcgta cttcatcaca ggatactccg gcagctccac
      181 gcccatcacg ctcttgaaca cggtgcggat catcgcttcc gtgatggcgc ggatctcctc
      241 ctcgccgagg aaggaggtct cgatatcgat ctgcgtgaac tcgggctggc ggtcggcgcg
      301 caggtcctcg tcgcggaagc acttggtgac ctggtagtag cggtcgaagc cggccaccat
      361 cagcagctgc ttgaagagct ggggcgactg cggcagcgcg aagaacatgc cgtcgtgcac
      421 gcgcgagggc acgaggtagt cgcgcgcacc ctcgggcgtg ctcttggtca gcatcggcgt
      481 ctcgatgtcg atgaagccct gggcatcgag gaacttgcgc acctccatcg ccacgcggta
      541 gcgcagcatc aggttcttct gcatgtaggg gcggcgcagg tccagcacgc ggtgcgtgag
      601 gcgcgtggtc tcggagaggt tctcgtcgtc gatctggaag ggcggcgtga cgcttgcgtt
      661 gagcacctcg agctcgtgcc ccagcacctc gatcttgccg ctggtgaggc cggcgttctc
      721 ggtgccagcc gggcgcgggc gcaccttgcc gacgatcttc aggcagaact cgttgcgcac
      781 gccctcggcg gtcttgaaca tctcggggcg gtcggggtcg cagacgatct gcaccaggcc
      841 ctcgcggtcg cgcaggtcga cgaagatcac accgccgtga tcgcgccggc ggtgagccca
      901 gcccatcagg gtcacggtct ggcccatcag cgcttcgctg acgaggccgc aataggtggt
      961 tcgcattcgg gtcactccag gaattcttgt cttgcttgcg caatgcgcgg acgacatggc
     1021 gccgcgaggg tgttcggggt aggcgctgcg ggggtcgtgg ccttgcggcc ctcaggactg
     1081 aacgcttgcc aggcgctcag cccccgagat ttcgcccgtc ggccagggcg gcgttggcgg
     1141 cggcctctgc gacctgcggc ggggctacca cgcccatcga gatgatgtat ttcagcgcct
     1201 cgtccacgct catctcgagt ttgatgacat cggcgcgcgg caccatcagg aagaagccgg
     1261 aagtcgggtt gggggtggtc ggaacgtaga ggctcagata gtcctcggcg tttaggtagt
     1321 ttgccacctc gcctccgggg cggccagtca cgaaggcgat ggtccaggcg ccgctgcgcg
     1381 ggtattgcac cagcacggct tcgcggaagg cattgccgct gctggagaac agcgtgtcgg
     1441 agacctgctt gaccgagctg tagatcgact tgacgatggg gatgtgattg agcagcctgt
     1501 cgccctggcg cagccaccac tggccgaaca tgttggccgc cagcatgccg gtcagcagca
     1561 gggccgccgc caccaccact aggctcagac ccggcaccct cttgagcgcc tccaggccct
     1621 cgcgtgcgct ttctggcagc acagcctggc cggccgatac cacggaggcg aacacgccgt
     1681 cgatcagccc catgacccac aggagcaccc agatcgtgat cgccagcggc agccagacca
     1741 gcaggccggc gatgatgtat ttcttcacgg gtttccttgg gccagcgtgc cggctgcttt
     1801 tttcttcaat ggcaggcgca ggaaccgccg cagggcttgg cgcccgcctc ggtgctgctg
     1861 ccgctcgaac cggacgcagg cgccgcagcg ctatcagccg gagcagtcgc ggcagaggcg
     1921 gatgtagcag ctgcgccgct gccattggct tggccgccat tgcggaaatc tgtcgcgtac
     1981 cagcccgagc ccttgagctg gaagcccggg gcggtgagct gtttcgcgaa ggtctcggcg
     2041 ccgcaggccg ggcaacggct cagcagcggg tcggagattt tctgcagcac gtcttgcgca
     2101 tggccgcacg aggcacagcg gtaagcgtag atcggcatgg ctaagctctt gatttgaaac
     2161 gcgaaattat agatgggccg gctgcggccg gtttcaaggg ggctcgtcgc cccactcagg
     2221 cctgggcttg agccgccggc gtcgacggcg caacgagctg cggcgccagc gagcccacca
     2281 ccatgccggc cagtgcagcg acgaggccgg ccagctgtgc cgggaaggcc tcgccccagt
     2341 tcgtgagctg aggcagcagg aagagcagcc agaccgccac gccgagcgtg atggacagga
     2401 tcgcgccctg ggtcgtcgcg cgcttccagt acaggcccgc cgccagcggc acgaaggccg
     2461 ccaccagcgt gacctgatag gccgaggaca cgaggtcgta gatcggggtg cccttcatcg
     2521 cgatcgcata gctgagcacc agcaccgcga agacaaagat cgtgatgcgc atggccagca
     2581 gcacctggcg gtcgcgcatg ccggggacca tgttcttcag gatgttctcg acgaagctgg
     2641 tgctgggtgc cagcagcgtg gccgaggagg tgctcttgat ggccgacagc agcgcgccga
     2701 agaagaggat ctgcatcacc agcggcatgt gggtcatgat gaagccgggc agcagcttct
     2761 ggtagtcgtt cttggccagc tccagggcat ggtcgcccat cacgaccacg gcgcagccga
     2821 cgatgaacat tggcacgaag gcgaagaaga tgtagctgac gccgccgatg gccgcgccgt
     2881 tgcgcgcggt ggccgcgtcc ttggccgaca tcacgcgctg gaacacgtcc tgctgcggaa
     2941 tgctgcccag catcatcgtg accgccgcgc cgacgaagaa ggtcacgtcc ttgaagctaa
     3001 cctcgggcat cagggtccac agctggcgct gcgaggccag cgccagcacc ttgcttggac
     3061 ccccggccag gttgccagcg aagatcgcga tgatggacag gccgatgacc agcacgatca
     3121 tctggatgaa gtcggtccag gccacggcga ggaagccgcc gatgatcacg tagaccagca
     3181 cggcgatcgt gcccaccacc atgccgacgg tctcgctgat cgcgccgttg gacagcaccg
     3241 agaacaccac gcccagcgcc gtgatctgtg cggccaccca gcccaggtag ctgaggatga
     3301 tggccaccga gcagaagatc tccacgccct tgccgaagcg ctggcggtag aagtcgccga
     3361 tggtcagcag gttcatgcgg tagagcttgg ccgcgaagaa cagacccacc aggatcaggc
     3421 aggtgccggc gccgaagggg tcttcgacga tggcgctcag gccgccctgc acgaacttgg
     3481 ccgggatgcc catcaccgtc tcggcgccga accaggtcgc gaaggtggtg gtgacgatca
     3541 tcgccagcgg caggctgcgc ccggcgatcg cgaaatcgct ggtgctgctg atgcgcgtgc
     3601 cggcccatac gccgatggcc agcgtgccga cgaggtagag gatgacgaag acgagcagcg
     3661 tgacgttcat gggaagccgg gcctagagca gggaaacgca gccgcaaatt atcgcggcat
     3721 cgaggcttgc aattccctgc cgctgcggcg gtaccggcac ggcaggcaaa tgaaaaatcg
     3781 agaagaattc ttcaagccac ccaaggaaat acggcgaata tcgatactga ttcgatagca
     3841 ccgtcaaacg ccctacggcg tgcgcctcga gcgcgcttct gcctagaaga aatgcacccg
     3901 cgtcatcacg agggtgagca ccagcccgat caggaagccc agtccgccat agacgacgcc
     3961 ctgcagcagc cggttggtgc ggcgctgctc tgccagcaaa gcggtcagct gcagctggtc
     4021 cggcgccgac gagaggccgc gcgccgtgcg ggccagcagc gcgtcgtgca gcaggcgcgg
     4081 cagctcaggc agcagctggg cgtagcgcgg cgcctcgttc ctgagctgcg tgagcagcgc
     4141 gcgccagccg atctgctcgt tcatccagcg ctctaggaag ggcttggcgg tgcgccacag
     4201 gtcgagctcg ggatcgagtt ggcggcccag gccctcgaca ttgagcaggg tcttctgcag
     4261 cagcaccagc tggggttgga tctcgacatt gaagcggcgc gaggcctgga acaggcgcag
     4321 cagcacctgg cccagcgaga tgtccttcag cggccggtcg aagtggggtt cgcagacggc
     4381 gcgcaccgcc ccttcgagct cgtcgacccg cgtgtcgcgc ggcacccagc cgctttcgac
     4441 gtgcagctcg gcgacgcgct tgtagtcgcg gcggaagaag gcgatgaag
//
LOCUS       DASUCY010000488         2025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_750035, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000488 DASUCY010000000
VERSION     DASUCY010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750035"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(337..1416)
                     /locus_tag="VFV25_09685"
     CDS             complement(337..1416)
                     /locus_tag="VFV25_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HEY1393637.1"
                     /translation="MTTRLAALRGPAAGDLFRALPGLALSGLLAAAAMAWGDTAWLQA
                     HGFSALTLAIALGILAGNTVYPRIAQSCGPGIAIAKQNLLRAGVVLYGLRLTVQDLGR
                     VGLSGVLVDALVLTSTFALAHWIGHRWLGLDRKTTMLIGAGSSICGAAAVMATERVVK
                     ARAEQVTVAVATVVVFGTLSVLLYPLLFELNKSWGFIPHGSQGFGIYAGSTIHEVAQV
                     LAAARAMDPAAADTALIAKMVRVMMLAPFLLILSAWMARGRSAGAGAATKSAKLAVPW
                     FAFAFVAVVLVNSLHGLPSRYKQPADVLDTALLAMAMAALGLSTHWSAIRKAGPKPLL
                     LALALFAWLVVGGAMINHGVALLLG"
     gene            1554..>2025
                     /locus_tag="VFV25_09690"
     CDS             1554..>2025
                     /locus_tag="VFV25_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY1393638.1"
                     /translation="MEVLRLTLRQLQIFAAVAHAGSTTAASEEIALSQSATSAAINEL
                     ERLLSLRLFDRTGKRLLLNENGRSLLPRALAVLRGALDIEQMAREPEAQMHSLRIGAS
                     TTIGNYALPGMLSQFLGTSARATGPGWHSAVSIGNTEAICEAVAAFELDIGLIEG"
BASE COUNT          378 a          701 c          646 g          300 t
ORIGIN      
        1 ggttcggcgc tcttccaggg gccggcctcc ttgtatgcct tgggcatttc catggccggc
       61 cgcacatagt tggggccggc ggcgcaggcg gccagcagca gcgatgccgc cgcgccaagc
      121 ggccacacca gccgcgagcg cagtgatggt ttcaaggaga agaagggaag catgagcggt
      181 tgcgccgcgc tcgagcgggc agggatgccc acgacgcaac ccctacaaat ctatgccccg
      241 ctacgagacg tcaggctgtc atgaagatga ccgttctgtc attttcgccg cgcctgcttg
      301 ggcatcactc cggtgcaggt attagggtcc gttgacctag cccagcagca gcgccacccc
      361 gtggttgatc atcgcgccgc ccaccacaag ccaggcgaag agcgccaggg ccaacagcag
      421 cggcttgggc ccggccttgc ggatcgcgct ccaatgcgtg ctcaggccca gtgctgccat
      481 cgccatggcc agcagcgcgg tgtccagcac atcggcgggt tgcttgtatc ggctcggcag
      541 cccatgcagc gagttgacga gcaccacggc cacgaaggcg aacgcgaacc aggggacggc
      601 aagtttcgca ctctttgtcg cggcgcccgc ccctgccgac ctgccccgcg ccatccaggc
      661 ggacaggatc agcaggaaag gcgcaagcat catcacccgc accatcttcg cgatgagggc
      721 cgtgtcggca gccgcagggt ccatggcacg cgccgccgcc agcacctggg ccacctcgtg
      781 gatggtcgag ccggcataga tgccgaagcc ttgggagccg tgcgggatga atccccagga
      841 cttgttcagc tcgaacaaga gcggataaag aaggaccgac agcgtgccga agaccaccac
      901 cgtcgccacc gcaaccgtga cctgctcggc gcgcgccttc acgacgcgct cggtcgccat
      961 caccgcagca gcgccgcaga tcgagctgcc cgcaccgatc agcatcgtcg tcttgcgatc
     1021 cagcccgagc cagcgatggc cgatccagtg ggccagcgca aaggtcgagg tcagcaccag
     1081 cgcgtcgacg agcacgccgc tcaggccgac tcgccccaga tcctgcaccg tcaggcgcag
     1141 gccatagagc acgaccccgg cgcgcaggag gttttgcttg gcgatggcga tgccgggccc
     1201 gcagctctgc gcgatgcgcg gatagacggt gttgcccgcc aggatgccca acgcgatagc
     1261 cagcgtcagc gcgctaaagc catgggcctg cagccaggcg gtgtcgcccc aagccatcgc
     1321 cgcagctgcc agcaagccgc tcaaggcaag gccgggcaag gccctgaaca agtcgcctgc
     1381 tgccggcccg cgcagcgcgg ccagccttgt cgtcatcggc aaatctctcg cggccacagc
     1441 gcaaatcccg ctctctgatt cgatggccgc aatatgcagt ctagcgctat gcctgcaaaa
     1501 acgacttgtt tggctactat ctagcaattg catcgataga tcgaagcccc atcatggaag
     1561 tgctcagact gaccctgcgc caattgcaga tctttgcggc cgtggcccac gccggcagca
     1621 ccacggcggc aagcgaggag atcgcgcttt cccagtcggc caccagtgcg gcgatcaacg
     1681 agctggagcg cctgctgtcg ctgcgcctct tcgaccgcac gggcaagcgc ctgctgctca
     1741 acgaaaacgg ccggagcctg ctgccgcgcg ccctggcggt gttgcgtggg gcgctggaca
     1801 tcgagcagat ggcccgcgag cccgaggcgc agatgcattc gctgcgcata ggcgccagca
     1861 ccacgatagg caactacgcg ctgcccggga tgctcagcca gttcctcggc acgagcgctc
     1921 gtgccacagg tccaggctgg cattcggcgg tgagcatcgg caacaccgag gcgatctgcg
     1981 aggccgtggc cgccttcgaa ctggacatcg ggctcatcga aggcc
//
LOCUS       DASUCY010000489         2223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1328070, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000489 DASUCY010000000
VERSION     DASUCY010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328070"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /locus_tag="VFV25_09695"
     CDS             <1..1062
                     /locus_tag="VFV25_09695"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEY1393639.1"
                     /translation="LDGDLLIVGGGDATLTADVLRAWFEELRRLGLREIGGNILLDRG
                     AFRLQPDDFAGTPAPSPRRPEHARPDAFSLRAAVPRLARSRPQQTQLAVHGPEPATSP
                     LRETYGGGVAPGATPNLAELALLAAGPASSRTIAALWARVGGVLRGRVVERKGEPEDG
                     DAMPRDADGERMLPFSVHHSASLAELLRGINKWSDNLAARNLMLTLSEGFPLRAATLP
                     DAKQRLRAWLAEQGLGEDDIAVDSGSGLSRAERGRARALAQLLSHAWHRPGADAFIDS
                     LPVAGVDGTLTHRLQDGEAAGRAFLKTGTLNDARALAGYVQGRSGKRYAVVAIVNDLR
                     ARAAVPVLDAFIEWVAHNG"
     gene            1095..1526
                     /locus_tag="VFV25_09700"
     CDS             1095..1526
                     /locus_tag="VFV25_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEY1393640.1"
                     /translation="MKRRNLLQTTLLAAGAAAAAQTADAQGSSAKHRVVFQVSDGDSA
                     KWNLALGNIRNMQDGVGADHVEIELVAYGPGIAMLKRDATTSDKVAEAVKSGVKVVGC
                     QNTMRAYKLASEDMNPAIGYVPSGVVELMTKQGEGWAYLRP"
     gene            1559..1632
                     /locus_tag="VFV25_09705"
     tRNA            1559..1632
                     /locus_tag="VFV25_09705"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1591..1593,aa:Gly,seq:ccc)
     gene            complement(1690..>2223)
                     /locus_tag="VFV25_09710"
     CDS             complement(1690..>2223)
                     /locus_tag="VFV25_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HEY1393641.1"
                     /translation="RALCPRTVELLAGIPSVKAAMFASLPPGSRLVRHRDPYAGSLRY
                     HLGLTTPNSDACYIEVDGQRYFWRDGEAVMFDETFIHYAENTTDQQRVILFCDVERPL
                     YLAPMRWFNRLFARIVMTASATQNEEGEPVGALNKFFFYAYKIRLAGKALKERSRTTY
                     YVGKWALIIGLLYWIFA"
BASE COUNT          381 a          727 c          766 g          349 t
ORIGIN      
        1 ctggatggcg atctgctgat cgtgggcggc ggcgatgcca ccctcaccgc cgatgtactg
       61 cgggcttggt tcgaagagct gaggcggctc ggcctgcgcg agatcggcgg caacatcctg
      121 ctggaccgcg gggcattccg cctacagccg gacgatttcg ccggcacgcc cgcgccttcg
      181 ccgcggcggc ccgagcacgc caggccggat gctttcagcc tgcgtgccgc cgtgccgcgc
      241 ttggcacgga gcaggccgca gcagacacag ctggcggttc atggccccga gcccgcaacg
      301 agccccttgc gcgagacgta cggcggtggc gtggcgccgg gcgcgacacc caacctcgcc
      361 gaacttgcgc tgttggctgc cggtccggca agctcgcgca ccatcgctgc gctgtgggcg
      421 cgtgtcgggg gcgtcctgcg gggccgggtg gtggagcgca agggcgaacc cgaggatggg
      481 gacgcgatgc cacgcgatgc cgatggcgag cgcatgctgc ctttctcggt tcaccattcg
      541 gcgtccttgg ccgagctgct gcgcggcatc aacaaatgga gcgacaacct ggccgcccgc
      601 aatctgatgt tgacactgtc cgaaggcttc ccgctgcgcg ccgccacgct gccggatgca
      661 aagcagcgcc tgcgggcctg gctcgccgaa caaggcctgg gcgaggacga tatcgcggtc
      721 gacagcggca gcggcctgtc gcgcgccgag cgcggcaggg cacgcgccct tgcgcagctg
      781 ctgagccacg cctggcatcg ccccggcgcc gatgccttca tcgacagctt gccggtggcc
      841 ggtgtcgacg gcacgctgac gcatcgccta caggatggcg aagcggcagg ccgcgccttc
      901 ctcaagaccg gtacgctgaa cgatgcgcgc gccctggctg gctatgtcca ggggcgcagc
      961 ggcaagcgct acgcggtggt cgccatcgtc aacgacctgc gagcacgggc tgcagtgccc
     1021 gtgctcgatg ccttcatcga atgggtggcg cacaatggct gattgccaat gagcatcagc
     1081 tggaagggcc gcacatgaaa cgccgcaatc tgctgcagac caccttgctg gcggcgggcg
     1141 ccgctgccgc agcgcagacg gccgacgcgc agggcagctc agccaagcac cgcgtcgtgt
     1201 tccaggtcag cgatggcgac tcggcgaaat ggaacctggc gctaggcaac atcaggaaca
     1261 tgcaggacgg cgtcggcgcg gaccatgtcg agatcgagct cgtcgcctac ggccctggca
     1321 tcgccatgct caagcgcgat gcaacgacca gcgacaaggt ggccgaggcc gtcaagtccg
     1381 gcgtcaaggt ggtgggctgc cagaacacca tgcgcgccta caagctcgcg agcgaggaca
     1441 tgaatcccgc gatcggctat gtgccttcgg gcgtcgtgga actgatgacc aagcagggcg
     1501 aaggctgggc ctatctgcgg ccctgaagcg agactccacg tatagaatcc gcatccgcgc
     1561 gggtgtagtt caatggcaga acggcagctt cccaagcttc atacgagggt tcgattccct
     1621 tcacccgctc cagatacgac aaagggcccc atgcgggccc tttgtgcttc cgtttgccga
     1681 ctgcgtcggc taagcgaaga tccagtagag caggccgatg atcaaggccc atttgccgac
     1741 gtagtaggtc gtgcggctgc gttctttaag cgccttgccc gccaggcgga tcttgtaggc
     1801 gtagaagaag aacttgttga gcgcgccgac cggctcgcct tcctcgttct gcgtggccga
     1861 ggccgtcatc acgatgcgcg cgaacagacg gttgaaccag cgcatcggcg ccaggtagag
     1921 cgggcgctcg acgtcgcaga acaggatcac gcgctgctgg tcggtcgtgt tctcggcgta
     1981 gtggatgaag gtctcgtcga acatcaccgc ctcgccatcg cgccagaaat agcgctggcc
     2041 gtcgacctcg atatagcaag cgtcggaatt gggcgtcgtc agccccaggt gatagcgtag
     2101 cgagccggcg tagggatcgc gatggcgcac caggcggctg cccggcggca gcgatgcgaa
     2161 catcgccgcc ttgacgctgg ggatgccggc cagcagctcc acggtgcgcg ggcacagcgc
     2221 gcg
//
LOCUS       DASUCY010000490         3430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_75740, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000490 DASUCY010000000
VERSION     DASUCY010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75740"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VFV25_09715"
     CDS             <1..248
                     /locus_tag="VFV25_09715"
                     /inference="COORDINATES: protein motif:HMM:NF027851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="general secretion pathway protein GspB"
                     /protein_id="HEY1393642.1"
                     /translation="ASAAEERIYAPADLPDEVRRSLPQLAIGGSVYSEAPGSRLLIVN
                     GQPLHEGDQPAPGVVLERIKLKSAVMSYKGYRYEISY"
     gene            complement(286..495)
                     /locus_tag="VFV25_09720"
     CDS             complement(286..495)
                     /locus_tag="VFV25_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393643.1"
                     /translation="MDDQASFEQVNGASLGRDDFRSSASIGLGPASRSPDDDDFAETR
                     PGCYSNSYLLGDADRHTLVGALRAA"
     gene            complement(836..2119)
                     /locus_tag="VFV25_09725"
     CDS             complement(836..2119)
                     /locus_tag="VFV25_09725"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEY1393644.1"
                     /translation="MKLASTAFCWALPGLLACLPGAAQAEADLSKPVQIVAARQPIPA
                     GQGGPKSFKFEFWKTLLKTNDVVGSWNYGLFCNDPKPLRAGKPLDDLLVSVISRAFYE
                     ELVKAGYSDSEQNKSLFDDKLGGGADYRVGATLIELDYRSCGRDTHKGSVYSKIKWEV
                     FSTRRQKVIYAAAIDASLQSEDKALPAEEFRRQVGRTIVNNLLGDPAFAEAVRTDGEA
                     GSAPDKTLSPLALDPKQTVSGVVMRNATPLLAAVVTIESAVGSGSGFFISQDGYLLTN
                     QHVVSDAKFVRVKLSNGHSMVGEVIRANKPRDVALVKTDPTASPVLQLRHGDARIGEE
                     VYALGSPYGEVLSGTLTKGVLSARRVFDGVAYLQSDVAINPGSSGGPLIDTEGAVLGI
                     AQSGASAKGINIFIPIDEALDKLALSLSTSAVAKP"
     gene            complement(2215..>3430)
                     /locus_tag="VFV25_09730"
     CDS             complement(2215..>3430)
                     /locus_tag="VFV25_09730"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEY1393645.1"
                     /translation="AGQPADALACADAALDALESFPPGHPLRPLAQAQAGLARVLIGH
                     IAAGRLEVEAALQAADAGYYANGPDGHSLLRQLDETLSRAGDAVGALEVYHREQGLRE
                     QRQRHDKEVALKDLQISYDSERKQREIDLLNRDNSLKAAAVEASELRQRLLWLAAVVL
                     GLGLLLAVLLHWRLRALRLDLAQRQTQLRRQSERDALTGLANRRHFEDRMAELLEARA
                     PGAGDGYEGALFMLDIDQFKRVNERLGHAGGDAVLAEVAARIAGTVRSKDIAARWGGE
                     EFVVAAPSLPAAQAEGLAERLLRAVGDHPVDVADTSLTVTVSIGYAHFPLPPHQPALP
                     WERALNLVDLALYVAKHRGRDRALGVTALHALDEASLQAVEAEFEPAWQDGRVHLHVL
                     QRAAPTAENKPS"
BASE COUNT          560 a         1121 c         1147 g          602 t
ORIGIN      
        1 ctgcctcggc cgccgaagag cgcatctacg cgccggccga tttgccggac gaggtgcggc
       61 gcagcctgcc gcagctggcg atcggcggtt cggtctactc ggaagctccg ggcagccggc
      121 tgttgatcgt caacggccag ccgctgcacg agggcgacca gccggcgccc ggggtggtgc
      181 tcgagcgcat caagctgaag tcggcagtga tgagctacaa gggctatcgc tacgagatca
      241 gctattagcc tggtctgacc cgggggcggc gaacgccgcc gctgctcagg ccgcgcgtag
      301 tgcgcccacc agcgtgtggc ggtcggcgtc gccgaggagg tagctgttgc tgtagcagcc
      361 gggccgggtc tcggcgaaat cgtcatcgtc tggcgagcgc gaagccggcc ccagtccgat
      421 cgaggccgag ctgcggaagt catcgcggcc gaggctggcc ccgttgacct gctcgaagga
      481 ggcttggtcg tccaaggcct gtacgggcgc tcccgccgcg cgtcggtgcg aagcaagaac
      541 tccgcgggct tgcagccact tcagtatcgt catcgcctgt atccctctgg gtccgccgca
      601 tcgcggcgac ggcactgggc ctgttaacgt tacgacaagc atcggcgaaa gtcgatgatc
      661 aggctatcca ccggaggggt gacaccaatg ggggatgtgc gcatttacaa cgccgatctg
      721 gcgtcggcgt cggccggctg ctgattgtca gcggccagtc gttgcggtgg tgctgagcta
      781 ctacacggga tatcgctagg agatcagcta ctagaccgat caggccgcgc atgcctcaag
      841 gtttggcgac tgcgctagtg gaaaggctta gcgccagctt gtccagcgct tcatcgatgg
      901 gaatgaagat attgatcccc tttgcgcttg cacccgactg tgcaatcccc agtactgcgc
      961 cttcggtgtc gatcagcggc ccgccgctgc tgcccggatt gatggcgacg tcgctttgca
     1021 gataagcgac gccgtcgaag acgcgccgcg cgctcagcac tcctttggta agcgtgccgc
     1081 tcaggacttc tccgtacggt gagccaagtg cgtagacctc ttccccgatg cgtgcgtcgc
     1141 catgccgaag ctgcagcact ggcgatgcgg tggggtcggt cttgaccaat gcgacatcgc
     1201 gcggtttgtt tgcgcgaatg acttcgccca ccatgctgtg tccattggac aatttgaccc
     1261 gcacgaactt ggcatcgctg acgacatgct gattggtcag cagatagcca tcctggctga
     1321 tgaagaagcc cgagccgctg cccacggcgc tctcgatggt gacgacagcg gccagcaagg
     1381 gagttgcatt gcgcatcacg acgccactga ccgtttgctt cggatcgagc gcaagagggc
     1441 tgagcgtctt gtccggggcc gagcctgctt ccccgtcggt gcgcacggcc tcggcgaacg
     1501 cagggtcgcc gagcaggttg ttcacgatcg tgcgaccgac ctgtcgccgg aactcctcgg
     1561 cgggcaaggc cttgtcttcc gactgcaagc ttgcatcaat ggcggcggca tagatgacct
     1621 tctggcggcg cgtcgaaaag acctcccact tgatcttcga gtacacactg cccttatgcg
     1681 tgtctcggcc gcagcttcga tagtccagtt cgatcaaggt ggcgccgacc cggtagtccg
     1741 caccaccgcc caacttgtcg tcaaagagcg atttgttctg ctcgctatcc gagtagccgg
     1801 ccttgaccaa ctcctcatag aaagcgcggg aaatcacact cactagcaga tcgtcgagcg
     1861 gcttgccggc ccgcagcggc ttggggtcgt tgcagaacag gccgtagttc caactgccga
     1921 cgacgtcatt ggtcttgagc agcgtcttcc agaattcaaa cttgaagctc ttcgggccgc
     1981 cttggcccgc cggaatcggc tggcgagccg cgacgatctg gacaggcttg gaaagatcgg
     2041 cctctgcctg cgccgccccc ggcaaacaag caagcaagcc gggcaaggcc cagcaaaagg
     2101 ctgtggaagc caacttcata tccggctcac ttcgcggctg aacatggcgc gcgatgatgc
     2161 cacgcgacaa ctcatgcggt cgagcctgcc cgtgtcatcg cccgctcccg acgttcaact
     2221 tggcttattt tcggcggtgg gtgccgcacg ctgcagcaca tgcaggtgca cgcgcccgtc
     2281 ttgccaggcc ggctcgaatt cggcctccac ggcctgcagc gaggcttcgt cgagggcatg
     2341 cagcgcggtc acgcccagcg cccggtcgcg gccacggtgc ttggcaacgt agagcgcgag
     2401 gtcgaccagg ttgagcgcgc gttcccaggg cagtgccggc tggtgcggcg gcagagggaa
     2461 atgggcgtag ccgatcgaca cggtcacggt caggctggtg tcggccacgt cgaccgggtg
     2521 gtcacccacg gcacgcagca ggcgctcggc caggccctcc gcctgggcgg ccggcaggct
     2581 gggcgcggcg acgacgaatt cctccccgcc ccagcgggcg gcgatgtcct tggagcgcac
     2641 cgtgccggcg atgcgcgccg cgacctcggc cagcacggcg tcgcctcccg catggcccag
     2701 acgctcgttg acgcgcttga actggtcgat gtcgagcatg aagagtgcgc cttcgtagcc
     2761 atcgcccgct ccgggtgcac gcgcctccag cagctccgcc atgcggtcct cgaaatggcg
     2821 ccggttggcc aggccggtca gggcgtcgcg ctcgctctgg cggcgcagct gggtctggcg
     2881 ctgggccagg tccagccgca gggcgcgcag gcgccaatgg aggagcaccg ccagtagcag
     2941 gcccagtccg agtaccacgg cggccaacca cagcaggcgc tggcgcagct cgctggcctc
     3001 tacggcggct gccttgagcg agttgtcgcg gttgagcagg tcgatctcgc gctgcttgcg
     3061 ttcgctgtca tagctgatct gcagatcctt gagagcgacc tccttgtcgt gacgctggcg
     3121 ctgctcgcgc agaccctgtt cgcggtggta gacctccagc gcaccgacgg cgtcgccggc
     3181 ccggctcaag gtttcgtcga gctggcgcag caggctgtgg ccatccggcc cattggcgta
     3241 gtagcccgca tccgcagcct gcagggccgc ttctacctcc aggcggccag ccgcgatatg
     3301 gccgatcagc acgcgcgcga ggccggcctg ggcctgggcc agggggcgca gcgggtgtcc
     3361 aggggggaag gattccaggg catccagcgc tgcatcggcg caggccaggg catcagcagg
     3421 ctgccccgca
//
LOCUS       DASUCY010000491         2342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_380868, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000491 DASUCY010000000
VERSION     DASUCY010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380868"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VFV25_09735"
     CDS             complement(<1..261)
                     /locus_tag="VFV25_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HEY1393646.1"
                     /translation="MFDPSDSRSAPMEELSAMVDGELDPAASARIATGWRDDGQVRAA
                     WHAYHLIGDVLRSEDLACAGRNEAFLRRVRARLAEEPVVLAPA"
     gene            complement(283..891)
                     /gene="rpoE"
                     /locus_tag="VFV25_09740"
     CDS             complement(283..891)
                     /gene="rpoE"
                     /locus_tag="VFV25_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HEY1393647.1"
                     /translation="MTANDADAALVARVKHGDVRAFEMLVVKYQRRIERLIGRMVRDN
                     DLVQDIAQETFIRAYRAMPQFRGESAFYTWLYRIAVNTAKKALVELKRDPIVTESALF
                     SRADDDETSRVENELSDGETPDAVLASKEIAATVNAAIEDLSEDLRQAITLREIEGLS
                     YEEIASVMNCPIGTVRSRIFRAREAIAQRLRPLLNTREGERW"
     gene            complement(986..1411)
                     /locus_tag="VFV25_09745"
     CDS             complement(986..1411)
                     /locus_tag="VFV25_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393648.1"
                     /translation="MLLAFSTMLGWAQARAAAPDAALAWGLFTVTLLLGAFVVITAWR
                     TPERHLRWDRQCWHLGWEQDPADPPVRGDLHVAVDLDAWMLLHFRPEGKRLGVWIALS
                     RRDLPDDWHALRCAVYSPRPQTSPGTGSAAPSDTKPPSA"
     gene            complement(1512..>2342)
                     /locus_tag="VFV25_09750"
     CDS             complement(1512..>2342)
                     /locus_tag="VFV25_09750"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEY1393649.1"
                     /translation="VPASIINMISGHVSIQCGFKGPNLAIVTACTTGLHSIGEAGRLI
                     EYGDADVMIAGGAESTVSPLGIGGFAAARALSTRNDDPATASRPWDKDRDGFVLGEGA
                     GVMVLEEYEHAKKRGAKIYAELAGFGMSADAYHMTAPNVDGPRRSMQAALRNAGLNAD
                     AVQYLNAHGTSTPLGDVNETNAIKLAFGDHAKRLVVNSTKSMTGHLLGGAGGIESVFT
                     ALAVHHQVSPPTINIFNQDPECDLDYCANAAREMKIDLALKNNFGFGGTNGSLVFKRV
                     "
BASE COUNT          399 a          788 c          761 g          394 t
ORIGIN      
        1 cgcaggtgcc agcaccacgg gttcctcggc caggcgcgcc ctgactcgcc gcaagaaggc
       61 ctcgttgcgg ccggcgcagg ccagatcctc cgaacgcagg acgtcgccga tcaggtgata
      121 ggcatgccag gcggcacgca cctggccatc gtcgcgccag ccggtggcga tgcgcgccga
      181 cgcagcggga tcgagctccc cgtccaccat ggccgacagc tcttccatgg gcgccgacct
      241 cgaatcggaa ggatcaaaca tcttcgtcac tccaggatgc attcaccagc gttcgccctc
      301 gcgagtgttc agcagcggcc gcagccgctg cgcaatggcc tcgcgcgcac ggaagatacg
      361 cgaacgcacg gtgccgatcg ggcaattcat cacgctcgcg atctcttcgt agctcagccc
      421 ctcgatctcg cgcagcgtga tggcctggcg cagatcctcg gacaggtcct cgatcgccgc
      481 gttgactgtg gccgcgattt ccttgctggc cagcactgca tcgggggttt cgccgtcgct
      541 tagttcgttc tcgacgcggg aagtttcgtc gtcgtcagcc cgcgaaaaca gcgccgactc
      601 ggtaacgatc gggtcgcgct tgagctccac cagggctttt ttggcggtat tgacggcaat
      661 gcggtagagc caggtgtaaa aggcactctc gccgcggaac tgcggcatgg cccggtaggc
      721 gcggatgaag gtttcctggg cgatgtcctg caccaggtcg ttgtcgcgca ccatgcgtcc
      781 gatcaggcgc tcgatgcgcc gctggtattt caccaccagc atctcgaagg cccgcacgtc
      841 gccgtgcttg acacgggcga ccagcgctgc gtcggcatcg ttggcggtca tcgctccacc
      901 ctagcgcggc gcgcctctcc tctatcggga aggagtgccc tggaagccac gaccgcggcc
      961 acgacccatg gcgactgttt ggggctcatg cggaaggagg tttcgtgtcg gaaggagcgg
     1021 ccgagccggt acccgggctg gtttgaggtc gcggcgaata taccgcgcag cgcagcgcat
     1081 gccagtcgtc gggcaagtcc cgccgggata aagcaatcca gactcccagc ctcttacctt
     1141 ccggccggaa atgcaacagc atccaggcat ccagatcgac tgcgacatgg agatcgccgc
     1201 gcactggcgg atccgccggg tcttgctccc agcccaggtg ccagcactga cggtcccagc
     1261 gcagatggcg ctcgggcgtg cgccacgccg tgatgacgac gaatgcaccg agcagcaagg
     1321 taaccgtgaa cagcccccag gcgagcgcgg catccggcgc tgccgcacgc gcctgcgccc
     1381 agcccagcat ggtgctgaac gccagcagca ccaccaggac ttggacgccc agccacaacg
     1441 aactgcgggg cgcacggacc gcgatggccg gcgccgtacg catcaggcct caagccggac
     1501 gggagcgcgc ctcaaacgcg cttgaacacc aacgagccgt tggtgccgcc gaagccgaag
     1561 ttgttcttca gcgccaagtc gatcttcatc tcgcgcgccg cattggcgca atagtccaga
     1621 tcgcactcgg gatcctggtt gaagatgttg atcgtcggcg gcgaaacctg gtgatgcacg
     1681 gccagcgccg tgaacaccga ctcgatcccg cccgcgccgc cgagcaggtg gccggtcatg
     1741 gacttggtgg agttcaccac cagccgcttt gcatgatcgc cgaaggcgag cttgatcgca
     1801 ttggtttcgt tgacgtcgcc cagtggcgtg gaggtgccgt gggcgttgag gtactgcacc
     1861 gcatcggcgt tgaggccggc attgcgcagc gcggcctgca ttgagcggcg cggaccgtcg
     1921 acattgggcg ccgtcatgtg ataggcgtct gcgctcatgc cgaagccggc caactcggcg
     1981 tagatcttcg cgccgcgctt cttggcgtgc tcgtattcct cgagcaccat cacgcccgca
     2041 ccttcgccca gcacgaagcc gtcgcgatcc ttgtcccagg gccgcgaggc ggtggccgga
     2101 tcgtcgttgc gcgtggatag cgcgcgcgct gccgcgaagc cgccaatgcc cagcggcgac
     2161 acggtggact cggcgccgcc ggcgatcatc acgtcggcat cgccgtattc gatcagccgg
     2221 cccgcctcgc cgatgctgtg caggccggtg gtgcaggcag tgacgatggc caggttaggc
     2281 cccttgaagc cgcattggat cgacacatga cccgagatca tgttgatgat cgaggccggc
     2341 ac
//
LOCUS       DASUCY010000492         2089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_332547, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000492 DASUCY010000000
VERSION     DASUCY010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332547"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..866)
                     /locus_tag="VFV25_09755"
     CDS             complement(147..866)
                     /locus_tag="VFV25_09755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY1393650.1"
                     /translation="MHLLLVEDDTLISRELSLRWRGRGWLVQACASLSDASAALAQQG
                     GFDLIVLDLGLPDGDGMDWLARLRQRDRNTPVLVLTARDRVVDRVRGLQGGADDYLVK
                     PFAADELDARVEVLTRRAEAARGEIAQYGLISWLGREGRAYVDGRALELSPREFEVLG
                     LLIRRAPKLVPKRVLIDALAERNLEVGDSAAEVYVSRLRRKLAGSGTVIRTLRGFGYL
                     LALDDGAAETTTDEEADAPTR"
     gene            complement(968..1906)
                     /locus_tag="VFV25_09760"
     CDS             complement(968..1906)
                     /locus_tag="VFV25_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEY1393651.1"
                     /translation="MSSATTATATAPLAAPRAGLSPLLIACLAATWLVWGSTYLAIKF
                     ALQSFPPFFQMGSRFLFAGALLLGWMRWRGAPWPSREQWRNALLIGALMLGGGMGGTA
                     YAEQSVGSGLVVAFIAILPAMMAAGQMLWRVYPSRLEAAGIAVGLAGVLLLTRGAGFQ
                     ASTAGLVAITLACLSWCAGSLLSQRRLPLAPGAMGFASEMICGGVVLLLLAVPDGEYP
                     KLAAHWPFEPGAVAAWAYLVVFGSLIAFNAYMVLLAQVSVALSSSYSFVNPVIAMLLG
                     VSLGGEVVSGLEWLAAGIVLASVMLLLAGRSSGKQH"
BASE COUNT          368 a          726 c          735 g          260 t
ORIGIN      
        1 cagccagcga tcgaagatgc ggtcggtcag gcgctgcgcc gtgtaggcgc cgatgccggc
       61 ggtcatcgcg acaatggcca ggagaggcaa catcagccgc agttgtagat cgcgcgccag
      121 ccagcgggcg cgcagcgggg cggtcatcat cgggtggggg cgtccgcttc ctcgtcggtc
      181 gtggtttctg cggcgccatc gtccagtgcc agcagataac cgaagccgcg cagcgtgcgg
      241 atcaccgtgc ccgagccggc cagcttgcgc cgcagccgcg acacatagac ctcggctgcg
      301 ctgtcgccga cttcgagatt gcgctcggcc agcgcgtcga tcagcacgcg cttgggcacc
      361 agcttgggcg cgcgccggat caggaggccc agcacctcga attcgcgcgg cgacagctcc
      421 agcgcgcggc cgtccacata ggcacggcct tcgcggccca gccaggagat cagcccgtat
      481 tgcgcgatct cgccgcgcgc ggcctcggcg cggcgcgtca gcacctcgac gcgggcatcc
      541 agttcatcgg cggcgaaggg tttgacgagg tagtcgtcgg ccccgccttg caggccgcgc
      601 acgcgatcga ccacgcggtc gcgcgccgtc agcaccagca ccggcgtgtt gcggtcgcgc
      661 tggcgcaacc gggccagcca gtccatgccg tcgccgtcgg gcaggcccag gtccagcacg
      721 atgagatcga agccgccctg ctgggccagc gccgcgctgg cgtcggaaag cgacgcgcag
      781 gcttgcacca gccagccgcg gccgcgccag cgcagcgaga gctcgcggga aatcagggtg
      841 tcgtcttcga ccagcagcag atgcattgcg acggcaatgg agcaggcatg ccgctgacga
      901 agcgccggcg gcttggcgag cgccaagcct actacgcccg cgggccgttt ccctggcgcg
      961 cgcccgctca gtgttgcttg ccgctcgaac gccccgccag cagcagcatc acgcttgcga
     1021 gcacgatgcc cgccgccagc cattcgaggc cgctgacgac ctcgccgccc aggctcacgc
     1081 ccagcagcat cgcgatcacc ggattgacga agctgtagct ggacgacaag gcgaccgaga
     1141 cctgcgccag cagcaccatg taggcattga aggcgatcag cgagccgaac acgacgaggt
     1201 aggcccaggc tgccacggcg cccggctcga agggccagtg cgcggcgagc ttgggatatt
     1261 cgccgtccgg cacggcgagc agcagcagga ccacgccgcc gcagatcatc tcgctggcga
     1321 agcccatcgc gcccggtgcg agcggcagcc tgcgctggct cagcaggctg ccggcgcacc
     1381 agctgaggca ggccagggtg atcgcgacca ggccggcggt cgaggcctgg aagccggcgc
     1441 cgcgcgtcag cagcagcacg ccggccagcc ccacggcgat gccggctgcc tccagccggg
     1501 aagggtagac gcgccacagc atctgacccg ccgccatcat ggccggcagg atggcgatga
     1561 aggcgaccac caggcccgag cccacgctct gctcggcata ggccgtgccg cccatgccgc
     1621 cgcccagcat cagcgcgccg atcagcagcg cgttgcgcca ctgctcgcgg ctgggccagg
     1681 gcgcgccgcg ccagcgcatc cagcctagca gcagcgcgcc cgcgaagaga aagcgcgagc
     1741 ccatctggaa gaaggggggg aagctctgca gcgcgaactt gatggccaga taggtcgagc
     1801 cccagaccag ccaggtggcg gccaggcaag cgatgagcaa aggcgacagg ccggcgcgtg
     1861 gtgcggccag cggggcggtg gcggtagcgg tggtggctga actcatgccg gcatgctagg
     1921 gggcagaggc gggtcgcttc gatagaagaa tcaaggaagt cggcctcgga aaatttgaaa
     1981 ttcaaggcaa tttgggctta cgctttgcgc atgaacggtg aaccagcgct agaccattac
     2041 gacacccgca tcctggccga gctgcaggcc gacgcacgcc tgagcatgg
//
LOCUS       DASUCY010000493         3280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1473029, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000493 DASUCY010000000
VERSION     DASUCY010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473029"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VFV25_09765"
     CDS             complement(<1..546)
                     /locus_tag="VFV25_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393652.1"
                     /translation="MEIGFEDDAPGFDSRAGQATAAAAAAADVLAAKPRTPVDTVHNE
                     LKAAEVLARFQAQRERWFAGLGEQTAGIERVLREEAAASDRLGGLPGTDHRAAWAPES
                     AAGEAAVSAPQPGDQAAEWQPPLWAMAPVLRSTPGNLRLKLPASLDAAALQRALVEHC
                     PPAWQRERVRVRLEIETRIYWG"
     gene            complement(654..2474)
                     /locus_tag="VFV25_09770"
     CDS             complement(654..2474)
                     /locus_tag="VFV25_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY1393653.1"
                     /translation="MTTARLAAEPSQQAELPIGQQPELPDHVRNLYTYTPSSLVGNAI
                     GAAVLVLLFAGAQPGLVSLWSAAFTVAWVYRAWLAWRFSAVGDQPDTDFVLWQVRWNI
                     GTLASGLLWGVAGWLFYNSGDAIQQIALIIIIYSYCVASVSVLADQKRVFIAFLLLNL
                     VPTIARIAREGSVYSYELVGVLCLAMAMTALLGRNYRQALERVIELKVRTEQLLRQLR
                     VEKQAADEARREAEVANRAKTQFFAAASHDLRQPLHALGLFAEALRQKSRGDGEVSQL
                     VNSINSSVDALEGLFSELLDITRIDTGGVEVQAENFSIRDLFARLRLAFEPTAFEKGL
                     ALDFRGEARYGHADPLLVERILRNLVSNAIRYTEDGGVLVSCRNRGDQLCLQVWDTGI
                     GIAEKEQPRIFEEFYQVHSAKPLEPHHRKGLGLGLAIVKRLANLMGAPLTVRSQPGRG
                     TVFTLLVTMGKPPRATGTVPSTKGPLGLTLTHRLIVVIEDEPAVREALEVLLKGWGAS
                     VVSFDTVEATRGWAELQDASLIKPDLMLVDYRLPGGSTGIDAITVLRQRFGPVPAIMI
                     TGSTMTGHEDDAQRHDFHVLIKPVVPNKLRAMIGFKLGVR"
     gene            complement(2476..>3280)
                     /locus_tag="VFV25_09775"
     CDS             complement(2476..>3280)
                     /locus_tag="VFV25_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393654.1"
                     /translation="ACLTFNVIANRELLKLMYECGCRSVFVGLETFNPATLADISKPQ
                     NNIAKMAQAIAQSRDEGILVIAGLIVSPIHDDEAYIRSLPDHLDDCGLHVPSFVSFET
                     PIPGTPFFDRMAAAQQPMFLPNANLYDFSAYTLVLQPQKTPLQAFLAAYGETMRRIYA
                     PRRRLAKLADDLPRLIRRGSWTGAALDLGDMWATNFDPVQGRTFMAGTDTLPPEVVPF
                     KPDDFESEAQWLDLISPTLVSDEHGRVLPHWRQHHKVFGVNLQSDLEAA"
BASE COUNT          546 a         1090 c         1108 g          536 t
ORIGIN      
        1 gccccaatag atgcgcgtct cgatctccag gcgcacgcgc acccgctcgc gctgccatgc
       61 aggcgggcaa tgctcgacca gcgcccgctg cagagccgcc gcgtcgaggc tggccggcag
      121 tttcaagcgc agattgcccg gagtggaacg cagcaccggg gccatcgccc acagcggcgg
      181 ctgccattcg gccgcctgat cgcctggctg cggcgccgag acggcggcct cgcctgcggc
      241 cgactcaggc gcccaggctg cgcgatgatc ggtaccaggc aggccgccca acctgtcgct
      301 ggccgctgcc tcttcgcgca gcacgcgctc gatgccagcg gtttgctcgc ccagacccgc
      361 gaaccagcgc tcgcgctggg cctggaagcg cgccagcact tccgcagcct tcaactcgtt
      421 gtgtacggta tcgactggcg tacgtggctt cgcggccagc acgtcggccg cagccgcggc
      481 agcagcagtg gcctgccccg cacgtgaatc gaagcccggc gcatcatcct cgaacccgat
      541 ttccaaatcc agggctgacg cgccagatcc aagcagacgc cgtggatccg gctccgccgg
      601 tccaccggcg tcaccccctt gagggggagc gccgaaggcg ctacgggggg gagtcatctc
      661 acgcccaatt tgaaaccgat catcgcgcgc agcttgttgg gcacgaccgg cttgatcagc
      721 acgtggaagt cgtggcgctg tgcatcgtcc tcatggccgg tcatggtgct gccggtgatc
      781 atgatggccg gcaccggccc gaagcgctgg cgcaggaccg tgatggcgtc gatcccggtg
      841 cttcccccgg gcaggcggta gtcgaccagc atcaggtcgg gcttgatcag gctcgcgtcc
      901 tgcagctcgg cccagccgcg cgtggcctcc acggtgtcga agctcaccac gctggcgccc
      961 cagcccttga gcaggacttc cagcgcctcg cgcactgctg gttcgtcctc gatcaccacg
     1021 atcaggcgat gcgtcagcgt caggcccagc ggccccttgg tcgagggtac ggtacccgtg
     1081 gcgcgcggcg gtttgcccat agtcacgagc agcgtgaaga ccgtgccgcg accgggctgc
     1141 gagcgcaccg tgagcggcgc ccccatcaga ttggccaggc gcttcacgat ggccagcccg
     1201 aggcccaggc ccttgcggtg atgcggctcc agcggcttgg cgctgtgcac ctggtagaac
     1261 tcctcgaaga tgcggggctg ctccttctcg gcgatgccga tgccggtgtc ccagacctgc
     1321 aggcatagct ggtcgccgcg attgcggcag ctcaccagca cgccgccgtc ctcggtgtag
     1381 cggatcgcgt tggacacgag gttgcgcagg atgcgctcca ccagcagcgg gtcggcgtgg
     1441 ccatagcggg cctcgccgcg gaaatcgagc gccaggcctt tctcgaaggc ggtgggctcg
     1501 aaggccagcc gcaggcgcgc gaagaggtcg cgaatcgaga agttctcggc ctgcacctcg
     1561 acgccgccgg tgtcgatgcg cgtgatgtcg agcagctcgc tgaacaggcc ctccagcgcg
     1621 tcgaccgagc tgttgatgct gttgaccaac tggctcacct cgccgtcgcc gcggctcttc
     1681 tgccgcagcg cctcggcgaa caagcccagc gcatgcagcg gctggcgcag gtcgtggctg
     1741 gccgcggcga agaactgggt cttggcgcgg ttggcgacct cggcctcgcg gcgcgcctcg
     1801 tcggcagcct gcttttccac ccgcaactgc ctgagcagct gctcggtgcg gaccttgagt
     1861 tcgatcaccc gctccagtgc ctggcggtag ttgcggccca gcagagccgt catcgccatc
     1921 gcgaggcaga gcacacccac cagttcgtag ctgtagaccg agccttcgcg ggcgatgcgc
     1981 gcaatggtcg gcacgaggtt gagcagcagg aaggcgatga agacgcgctt ctggtcggcc
     2041 agcaccgaca ccgaggccac gcaatagctg tagatgatga tgatcagtgc gatctgctgg
     2101 atcgcatcgc ccgagttgta gaacagccag ccggctacgc cccacagcag gccagacgcc
     2161 agcgtgccaa tgttccagcg cacctgccac agcacgaaat cggtatcggg ctgatctccc
     2221 accgcactga atcgccaagc cagccacgcc cggtacaccc atgccaccgt gaaggcggcg
     2281 ctccataagc tcacaaggcc cggctgggct cctgcgaaaa gcagaacgag cactgccgcg
     2341 ccgatcgcgt tgccgaccag gctggacggt gtataggtat agaggttgcg cacatggtcc
     2401 ggcaactcgg gctgctgacc gatcggcagt tcggcttgtt ggctgggctc ggcagcgagt
     2461 cgggctgtcg tcatctcatg cagcttcgag atcggattgc aggttcacgc caaacacctt
     2521 gtgatgctgg cgccagtgcg gcagcacacg gccgtgctcg tcgctgacca gagtggggct
     2581 gatcaggtcc agccactgcg cctcggattc gaaatcgtcg ggcttgaagg gcacgacctc
     2641 gggcggcagt gtatcggtgc cggccatgaa ggtgcggccc tgtaccgggt cgaaattggt
     2701 ggcccacatg tcaccgaggt cgagtgcggc acctgtccac gagccgcggc ggatcaggcg
     2761 cggcagatcg tcggcaagct tggccagacg gcggcgcggg gcatagatgc gccgcatggt
     2821 ctcgccatag gccgcgagga aagcctgcaa cggtgtcttc tgcggttgca gcaccagcgt
     2881 gtaggcgctg aagtcgtaga gattggcgtt gggcaggaac atcggctgct gagccgcagc
     2941 catgcgatcg aagaagggcg tgccggggat cggcgtctcg aaggacacga agctcggcac
     3001 atgcaggccg cagtcatcga ggtgatccgg cagcgaacgg atgtaggcct cgtcgtcgtg
     3061 gatgggactc acgatgaggc ccgcgatgac gaggatgccc tcgtcgcggc tctgggcgat
     3121 ggcctgcgcc atcttggcga tgttgttctg cggcttgctg atatcggcca gcgtcgcggg
     3181 attgaaggtc tccagcccta cgaacaccga gcggcaaccg cactcgtaca tcagcttcag
     3241 cagctcgcgg ttggcgatca cgttgaaggt caggcaggcg
//
LOCUS       DASUCY010000494         5221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1167864, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000494 DASUCY010000000
VERSION     DASUCY010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 5221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5221
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1167864"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /gene="fdhD"
                     /locus_tag="VFV25_09780"
     CDS             complement(<1..648)
                     /gene="fdhD"
                     /locus_tag="VFV25_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEY1393655.1"
                     /translation="MMMSPGIDGVPGAATLQALACHGGIPRMVSDWVADEVPVALQYN
                     GISHAVMLATPLDLEDFAYGFSLGEGIIERAGDLHEIEVEPVDDGIVLHVEVASRCLA
                     RLKERRRTLAGRTGCGLCGTESLAQALPRIQSQVAELAIEVDALSRALDGMRRHQALQ
                     SATGATHAAAWCDASGDVLLLREDVGRHNALDKLVGALARLQHDARAGFFAVTSRA"
     gene            complement(645..3479)
                     /gene="fdhF"
                     /locus_tag="VFV25_09785"
     CDS             complement(645..3479)
                     /gene="fdhF"
                     /locus_tag="VFV25_09785"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HEY1393656.1"
                     /translation="MQTSQDTDYGTPKRESVAAVTLEIDGQTVTVPAGTSLMRAAIEA
                     GIGVPKLCATDSLEPFGSCRLCLVEIEGRRGFPASCTTPAEAGMKVRTQSDKLGKLRH
                     DVMELYISDHPLDCLTCAANGDCELQDMAGAVGLRNVRYGFDGASHLSAKKDESNPYF
                     TYDPSKCIVCNRCVRACEETQGTFALTISGRGFESRVSPGQDQAFMDSECVSCGACVQ
                     ACPTATLQDKSVVSLGMPTKSVITTCAYCGVGCGFKAEVKGEQVVRMTPWKDGQANDG
                     HSCVKGRFAWGYATHKDRITKPMIRKKITDPWQEVSWDEAIAHAASEFRRIQGAHGRD
                     SIGGITSSRCTNEETYLVQKLIRAAFGNNNVDTCARVCHSPTGYGLKQTFGESAGTQT
                     FKSVEKSDVIMVIGANPTDAHPVFASRMKKRIREGARLIVVDPRRIDLVSSPHVKADY
                     HLQLRPGTNVAVVTALAHVIVTEGLLAEEFIAERCEEKAYRQWCDFVARSENSPEAME
                     AVTGVPAEALRGAARLYATGGNAAIYYGLGVTEHAQGSTTVMGIANLAMATGNVGREG
                     VGVNPLRGQNNVQGSCDMGSFPHELPGYRHVSDSIVRAEFDAAWGVTLPAEPGLRIPN
                     MFDAALEGSFKGLYVQGEDIVQSDPNTQHVTEALSRLECLVVQDIFLNETAKYAHVFL
                     PGSSFLEKDGTFTNAERRISRVRKVMKPLGGLGDWEVTIKLANALGYPMSYTHPSQIM
                     DEIASLTPSFHGVSYARIDKLGSVQWPCNEDTAEGGTPIMHVDEFVRGKGRFVITQYV
                     ATPEKVTRKFPLVLTTGRILSQYNVGAQTRRTPNNLWHEEDRLEIHAHDAEERGIRDG
                     DWVGIRSRAGETVLRAKVSDRVQPGVVYTTFHFPESGANVITTDSSDWATNCPEYKVT
                     AVQVTKVTQPSDWQRDYSRFNEAQLELLGK"
     gene            complement(3492..5030)
                     /locus_tag="VFV25_09790"
     CDS             complement(3492..5030)
                     /locus_tag="VFV25_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEY1393657.1"
                     /translation="MSVTVYVSRDAAALAMNADVVAAAIAGRASARRQEVNIVRNGSR
                     GLLWLEPMVEVATPAGRVAYGPVRVEDVASLFEANFLAGGEHPLRLGSTEDIPEFKHQ
                     QRLSFARCGQDHPLAPVSWTGLKTALEVPPEAIVQEVLDSGLRGRGGAAFPAGIKWRT
                     VAQTPSAQKYVVCNADEGDSGTFADRMLLEGDPYLLIEGMAIAALAVGATRGFVYIRS
                     EYPHAIATFQAACDAAREAGVLGPSVLDSGVSFDIEVRVGGGSYVCGEETAMLESLEG
                     KRGIVRAKPPLPAVQGLFGRPTIINNVLTFAAVPTIMEKGAEFYKAFGSGRSRGTLPF
                     QLAGNIRRGGLFELPFGLSLRELVFDIGGGTASGRPVKAIQVGGPLGAYVPESQWDLP
                     LDYDVYTAKGLVLGHGGIVVHDDTADMAQLARFAMEFCAIESCGKCTPCRIGSTRGVE
                     VIDRIVLNKNRPQQVRLLRDLCDTMLAGSLCAMGGMTPYPVLSALDHYPRDFGLVEDD
                     EQAA"
     gene            complement(5027..>5221)
                     /locus_tag="VFV25_09795"
     CDS             complement(5027..>5221)
                     /locus_tag="VFV25_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009771541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEY1393658.1"
                     /translation="ESCRSVGGEALLEQARAAGRCTVEPVYCLGLCALSPAVMLGGQP
                     HARVTPARLDELLDEAGDFQ"
BASE COUNT          799 a         1790 c         1765 g          867 t
ORIGIN      
        1 ggcgcggctg gtcaccgcga agaagcccgc gcgcgcatcg tgctggaggc gggcgagcgc
       61 gcccaccagc ttgtcgagcg cattgtggcg gcccacgtcc tcgcgcagca gcagcacgtc
      121 gccggaggca tcgcaccacg cggcggcgtg ggtcgcgccg gtcgcggact gcagtgcctg
      181 atgccgccgc atcccgtcca gggcacgcga cagcgcgtcg acctcgatgg ccagctcggc
      241 cacctgcgac tgaatgcgcg gcagggcctg cgccaggctt tcggtgccgc acaggccgca
      301 gcccgtgcgg ccggccagcg tccggcggcg ctccttcagg cgcgcgagac agcggctggc
      361 gacctcgaca tgcagcacga tgccatcgtc caccggctcg acctcgatct cgtgaagatc
      421 gcctgcgcgc tcgatgatgc cttcgcccag agagaagcca taggcgaagt cttcgagatc
      481 gagcggcgtg gccagcatca ccgcatgcga aatgccgttg tactgcaagg ccaccggcac
      541 ctcgtcggcg acccagtcgc tcaccatgcg cgggatgccg ccgtggcagg ccagcgcctg
      601 caacgtggcg gctccgggaa cgccgtcaat gcccgggctc atcatcattt gcccagcagc
      661 tcgagctgcg cttcgttgaa gcggctgtag tcgcgctgcc aatcggacgg ttgcgtcacc
      721 ttcgtcacct ggacagccgt gaccttgtac tccgggcaat tggtcgccca gtcggaactg
      781 tcggtggtga tgacgttggc gccggactcc gggaaatgga aggtggtgta gaccaccccg
      841 ggctgcacgc gatcgctgac tttggcacgc agcaccgtct cgcccgcgcg gctcctgatg
      901 ccgacccagt cgccgtccct gatgccgcgc tcctcggcgt cgtgagcgtg gatctccagc
      961 cggtcctcct cgtgccacag gttgttcggc gtgcgtcgcg tctgcgcacc gacgttgtac
     1021 tggctgagga tgcggcccgt ggtgagcacc agcgggaact tgcgcgtgac cttctccggc
     1081 gtcgccacgt actgggtgat gacgaagcgc cccttgccgc gcacgaactc gtcgacgtgc
     1141 atgatcggcg tgccgccttc ggccgtgtcc tcgttgcagg gccactgcac gctgccgagc
     1201 ttgtcgatcc tggcatagct cacgccgtgg aagctgggcg tgagcgatgc gatctcgtcc
     1261 atgatctgcg aggggtgcgt gtagctcatc gggtagccca gcgcattggc gagcttgatc
     1321 gtcacctccc agtccccgag gccgcccagc ggcttcatca ccttgcgcac gcgcgagatg
     1381 cggcgctcgg cgttggtgaa ggtgccgtcc ttctcgagga aactggaacc cggcaggaag
     1441 acgtgcgcat acttcgccgt ctcgttgagg aagatgtcct gcacgaccag gcattcgagc
     1501 ctggacagcg cctcggtcac gtgctgggtg ttggggtcgg actgcacgat gtcctcgcct
     1561 tgcacgtaca ggcccttgaa gctgccctcc agcgcggcgt cgaacatgtt gggaatgcgc
     1621 aggcccggct cggcgggcag ggtcacgccc caggccgcat cgaactcggc gcgcacgatg
     1681 ctgtccgaca cgtggcggta gcctggcaac tcgtgtggga acgagcccat gtcgcacgag
     1741 ccctgcacgt tgttctggcc gcgcagcggg ttcacgccca cgccttcgcg cccgacgttg
     1801 ccggtggcca tcgcgaggtt ggcgatgccc atcaccgtgg tcgagccttg cgcgtgctcg
     1861 gtgacgccca gaccatagta gatcgctgcg ttgccgccgg tggcgtacag ccgcgccgcg
     1921 ccgcgcagag cttcggccgg cacgccggtg acggcctcca tcgcctcggg cgagttttcg
     1981 ctgcgggcga cgaagtcaca ccattgccga taagccttct cctcgcagcg ttcggcgatg
     2041 aactcctcgg ccagaagacc ctcggtgacg atcacatgcg ccaaagcggt gacgacggcc
     2101 acattggtgc ccggcctgag ctgcaggtgg tagtcggcct tgacgtgggg cgaagacacc
     2161 aggtcgatgc gccgcgggtc cacgacgatc agcctggcgc cctcgcggat gcgcttcttc
     2221 atgcgcgagg cgaacaccgg atgagcgtcg gtcgggttgg cgccgatcac catgatgacg
     2281 tcggacttct ccaccgactt gaaggtctga gtgcccgccg attcgccgaa ggtctgcttc
     2341 aggccgtagc cggtgggcga atggcagacg cgggcgcagg tgtcgacgtt gttgttgccg
     2401 aaggccgcgc ggatgagctt ctgcacgagg taggtctcct cgttcgtgca gcgcgaagag
     2461 gtgatgccgc cgatcgaatc gcggccatgt gcgccctgga tgcgccggaa ttcgctggct
     2521 gcgtgcgcaa tggcctcgtc ccagctcacc tcctgccacg gatcggtgat cttcttgcgg
     2581 atcatcggct tggtaatacg gtccttgtgc gtcgcatagc cccaggcgaa gcggcccttc
     2641 acgcaggaat gaccatcgtt ggcctgacca tccttccagg gcgtcatgcg cacgacctgc
     2701 tcacccttga cctcggcctt gaagccgcag cccacgccgc agtaggcgca ggtggtgatc
     2761 acgctcttgg tcggcatgcc cagcgagacg acgctcttgt cctgcagcgt cgcggtgggg
     2821 caggcctgca cgcaggcgcc gcagctcacg cactcgctgt ccatgaaggc ctggtcctgg
     2881 cccggcgaca cgcgcgactc gaagccacgg cccgagatcg tcagtgcgaa ggtgccctgc
     2941 gtctcctcgc aggctcgcac gcaacggttg cagacgatgc acttgctcgg gtcataagtg
     3001 aagtacgggt tcgattcgtc cttcttcgca ctcaggtggc tggcgccatc gaagccatag
     3061 cgcacattgc gcaggcccac ggcgccggcc atgtcctgca gctcgcagtc gccgttggcg
     3121 gcacaggtga ggcagtcgag cgggtgatcg gagatgtaca gctccatcac gtcgtgccgc
     3181 aacttgccga gcttgtcgct ttgcgtgcgc accttcatgc cggcctcggc cggcgtcgtg
     3241 cacgaggccg ggaagccgcg ccggccctcg atttccacca gacacaggcg gcaggagccg
     3301 aagggctcca ggctatcggt cgcgcacagc ttgggcacgc cgatgccggc ctcgatggct
     3361 gcgcgcatca gcgaagtgcc ggccggcacg gtgacggtct ggccgtcgat ctcgagcgtc
     3421 accgcggcga cggactcgcg cttcggggtg ccgtagtcgg tgtcctggga tgtctgcatg
     3481 atggtggttg ttcaggcggc ttgctcgtcg tcttcgacca ggccgaagtc ccgtgggtag
     3541 tgatcgagcg cagaaagcac cggatacggc gtcatgccgc ccatcgcgca caggctgccg
     3601 gcgagcatgg tgtcgcacag gtcgcgcagc agccgcactt gttgcgggcg gttcttgttg
     3661 agcacgatgc ggtcgatcac ctcgacgccg cgcgtggagc cgatgcggca gggcgtgcac
     3721 ttgccgcacg actcgatggc gcagaactcc atcgcgaatc tcgccagctg tgccatgtcg
     3781 gcggtgtcgt cgtgcacgac gatgccgccg tggccgagca ccaggccctt ggccgtgtag
     3841 acgtcatagt ccagcggcag gtcccattgc gactcgggaa cgtaggcgcc cagagggccg
     3901 cccacctgga tggccttcac gggccggccg ctcgccgtgc cgccgccgat gtcgaacacc
     3961 agctcgcgca agctgaggcc gaagggcagc tcgaacaggc cgccgcgcct gatgttgccg
     4021 gcgagctgga agggcaaggt gccgcgcgag cggccgctgc cgaaggcctt gtagaactcg
     4081 gcgccctttt ccatgatggt cggcaccgcg gcgaaggtca gcacgttgtt gatgatcgtg
     4141 ggccggccga acaagccttg caccgcgggc agcggcggct tggcgcgcac gatgccgcgc
     4201 ttgccctcca ggctctcgag catcgcggtt tcttcgccgc acacgtagga gccaccgccc
     4261 acgcgcactt cgatgtcgaa ggacacgccg gagtccagca ccgagggccc aagcacaccg
     4321 gcttcgcgcg ccgcgtcgca ggcggcctga aaggtggcga tcgcgtgcgg atactcggaa
     4381 cggatgtaga cgaagccgcg cgtggcgccg actgccagcg ccgcgatggc catgccctcg
     4441 atcagcaggt aggggtcgcc ctccagcagc atgcggtcgg cgaaggttcc ggaatcgccc
     4501 tcgtccgcat tgcacaccac gtacttctgc gccgacggcg tttgcgccac cgtgcgccac
     4561 ttgatgcccg ccggaaaggc cgcgccaccg cggccgcgca gaccggagtc cagcacctcc
     4621 tgcacgatcg cttcaggggg cacctccagc gcggtcttca ggcccgtcca cgacaccggc
     4681 gcaagcgggt ggtcctggcc gcagcgcgcg aagctcaggc gctgctggtg cttgaactcg
     4741 gggatgtcct ccgtcgagcc caggcgcagc ggatgctcgc cgcccgcaag gaagttggcc
     4801 tcgaacagcg aggccacgtc ttccacgcgc accgggccgt aggcaacgcg gccggccggc
     4861 gtcgccactt cgaccatggg ctccagccac agcaggccgc gggagccgtt gcgcacgatg
     4921 ttcacttcct gccggcgcgc gctggcccgg ccggcgatcg cggccgccac cacatcggcg
     4981 ttcatcgcca gcgcggcggc gtcgcgggac acgtagacgg tgacgctcat tggaagtctc
     5041 ccgcctcgtc cagcagctca tcgagccggg ccggggtgac acgggcgtga ggctggccac
     5101 cgagcatcac cgctggcgac aaggcgcaca gccccaggca atacaccggc tccaccgtgc
     5161 agcggccggc cgcgcgggcc tgctccagca gcgcctcgcc gcctaccgag cggcacgatt
     5221 c
//
LOCUS       DASUCY010000495         2826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1152140, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000495 DASUCY010000000
VERSION     DASUCY010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152140"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="VFV25_09800"
     CDS             complement(<1..610)
                     /locus_tag="VFV25_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY1393659.1"
                     /translation="MNTSVTPEAQTSPAERLQAAGPCLNCIVASVVVLAAIVASFLYL
                     GIDYRSLFAAKSLRLMGQFVAEFFPPDLSAGFLAKVGRGCLQTLAVSALGTLLAVAAS
                     SVLALPASGRFGSAARWAARLLLNLLRSVPELVWAVLMVLAAGLGPFAGTLALALHTS
                     GVLGRLYAETLENAPREPELALRHAGGGAVAAFAYGTLPQVTA"
     gene            complement(613..1458)
                     /locus_tag="VFV25_09805"
     CDS             complement(613..1458)
                     /locus_tag="VFV25_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY1393660.1"
                     /translation="MTNTAGQLALPAERDPQAKRRLTGLLVAIVVLWPLLVLAEFKPW
                     ALFDAGNLKVMAGFLAGFLPPASTADFLGLLLKATLETLAMATAGIALAFVIAVPLAL
                     ATTRSLSISRIGPGPGRWFGQTLRQVTRALMVLLRSIPEVVWALVFVRALGLGPAAGV
                     LALAITYGGMLAKVYAEILDSGDTRPARALLEAGSGRLAALFYGLLPGAAQELASYTV
                     YRWECAVRASVVMGFVGAGGLGQLMDQSMKMLNGGEVSSILLCFLLLVLLADALSAAL
                     RRLLA"
     gene            complement(1458..2300)
                     /locus_tag="VFV25_09810"
     CDS             complement(1458..2300)
                     /locus_tag="VFV25_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY1393661.1"
                     /translation="MSFALDSVGLAHANGQRALRDVTLSAAAGERLAIIGPSGAGKTT
                     LLRLLATSLRPSEGRVQMLGANPWQLTARALKRLRARIGVVHQAPPIPPRLRVVTAVL
                     AGRLGSWPWPKALMSLLYPSDIAGAREVLARLDLADRLFERCDRLSGGQLQRVGIARA
                     LYQRPDLLLADEPVSALDPALANAAVSRLIEQAEASGATLVSSLHAVDLALKWFPRIV
                     GLRDGAVIFDASAPEVTSAMLYELYANEGRALPMQGDDVAMLGAGALAAGANVVPLVR
                     PGCR"
     gene            complement(2332..>2826)
                     /gene="phnD"
                     /locus_tag="VFV25_09815"
     CDS             complement(2332..>2826)
                     /gene="phnD"
                     /locus_tag="VFV25_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEY1393662.1"
                     /translation="SSAKSLADLKGKNFVFGSPSSTSGHLMPRYFLMKAGIDADKDFK
                     AVAFSGAHDATVAYVASGRAEAGVLNASVWDKLVEAHNPNAAKVRVLATTPPYFDYNW
                     TVRPGLDATLTKKLTAAFLKLDPANPEHKEIMALQRASKFIPTQASNYDGIEAAGKAA
                     GLIK"
BASE COUNT          498 a          979 c          985 g          364 t
ORIGIN      
        1 gagccgtcac ctgaggcaag gtaccgtacg caaaggcggc gacggcgccg ccgcctgcat
       61 ggcgcagggc cagctctggc tcgcgcggcg cgttctccag cgtctcggca tagagccggc
      121 ccagcacgcc gctggtgtgc aatgcaaggg ccaaagtgcc ggcaaagggc ccgaggccgg
      181 cggccagcac catcagcacg gcccagacca gctcgggcac gctgcgcagc aggttgagca
      241 gcaggcgcgc cgcccagcgc gcggcgctgc caaaacggcc cgaagccggc agggccagca
      301 cgctgctcgc cgccacggcg agcagcgtgc ccagggccga cacggccagg gtctgcaggc
      361 agccccggcc gacctttgcg aggaagccag ccgacagatc cggcgggaag aactcggcga
      421 cgaactggcc catcagcctc agcgatttgg ccgcgaacag tgaacggtag tcgatgccga
      481 ggtagaggaa gctggcgacg atcgccgcca gcaccacgac gctcgccacg atgcagttca
      541 ggcaggggcc ggccgcctgc aacctctcag cgggactggt ctgggcttca ggcgtcacgc
      601 tcgtgttcat gatcaggcga gcaggcggcg taaagccgcg ctcaaggcat cggccaacag
      661 caccagcagc aggaagcaca gcaggatgct gctgacttcg ccgccgttga gcatcttcat
      721 cgactggtcc atcagctgcc ccaggccgcc ggcgccgacg aaacccatca cgaccgaggc
      781 ccgcacggcg cattcccagc gatacacggt gtaggaagcg agctcctgcg cggcgcccgg
      841 cagcagcccg tagaacaaag cggcgagccg gccgctgccg gcttcgagca gggcgcgcgc
      901 gggccgggtg tcgccggaat cgaggatctc ggcatagacc ttggccagca tgccgccata
      961 ggtgatcgcc agcgccagca cgcccgccgc ggggccgagc cccagcgcgc gcacgaagac
     1021 cagggcccac actacttcgg gaatcgagcg cagcagcacc atcagcgcgc gcgtcacttg
     1081 gcgcagggtc tggccgaacc atcgccccgg tccggggccg atgcgcgaga tcgagagcga
     1141 gcgcgtcgtc gcgagcgcga gcgggacggc gatgacgaag gccagcgcga tgccggccgt
     1201 tgccatcgcc aaggtctcca gcgtcgcctt gagcagcaaa ccgaggaagt cggccgtgct
     1261 ggcgggcggt aggaaaccgg cgaggaagcc ggccatgacc ttgagattgc cggcatcgaa
     1321 caaggcccag ggcttgaact cggccagcac caggagcggc cacagcacga cgatggccac
     1381 gagcaagccg gtcaggcgac gcttcgcctg cggatcgcgt tcggcaggca aggcgagctg
     1441 gccggctgta ttcgtcatca tcggcaacct gggcgcacca gcggcaccac gttggcgcca
     1501 gcagcgagtg caccggcccc gagcatggcc acatcgtcgc cctgcatcgg cagcgccctg
     1561 ccctcgttcg catagagctc atacagcatc gcgctcgtca cttcaggagc cgaggcatcg
     1621 aagatcacgg ccccatcgcg caggccgacg atgcgcggga accacttcag cgccaggtcc
     1681 accgcgtgca gcgaggaaac cagggtcgca ccgctcgcct cggcctgctc aatcagccgg
     1741 ctcaccgccg cattggcaag cgccgggtcc agggccgaca cgggctcgtc ggcgagcagc
     1801 agatcggggc gctggtagag ggcgcgggcg atgcccacgc gctgcagctg tccgccggac
     1861 aggcggtcgc aacgctcgaa gagccggtcg gccaggtcca gccgcgccag cacttcgcgc
     1921 gcgccggcga tgtcgctggg atacagcagg gacatcaggg ccttgggcca gggccaggaa
     1981 ccgagccggc ccgccagcac ggcggtgacg acgcgcaggc gcggcgggat cggcggcgcc
     2041 tgatgcacga cgccgatgcg cgcgcgcaag cgcttcagcg cgcgggccgt gagctgccag
     2101 gggttggcgc ccagcatctg cacacggccc tcgctggggc gcaacgaggt cgccaagagg
     2161 cgcagcagcg tcgtcttgcc cgcgcccgag gggccgatga tggccaagcg ctctccggcc
     2221 gctgcgctca gcgtgacgtc acgcagcgcg cgctggccgt tcgcatgggc caggccgacg
     2281 ctgtccagcg cgaaactcat cgcctgcctg cctcgcaccg cgccatgtgt ttcatttgat
     2341 cagcccggcc gccttgccgg ccgcctcgat gccgtcgtag ttcgaggcct gggtcgggat
     2401 gaacttggat gcgcgttgca gcgccatgat ttccttgtgc tcggggttgg ccggatcgag
     2461 cttgaggaaa gccgccgtga gcttcttggt cagcgtcgca tccaggccgg gccgcaccgt
     2521 ccagttgtag tcgaagtagg gcggcgtcgt ggccaacacg cgcaccttgg cggcgttggg
     2581 gttgtgtgcc tcgaccagct tgtcccacac cgaggcattg agcacgcccg cctcggcgcg
     2641 ccccgaggcg acataggcaa cggtggcgtc atgcgccccg gagaaggcca cggccttgaa
     2701 gtccttgtcc gcatcgatgc cggccttcat caggaagtaa cgcggcatca gatggccaga
     2761 ggtcgacgaa ggcgagccga agacgaagtt cttgcccttc aggtccgcaa ggctcttggc
     2821 cgagct
//
LOCUS       DASUCY010000496         2896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1312521, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000496 DASUCY010000000
VERSION     DASUCY010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1312521"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VFV25_09820"
     CDS             <1..378
                     /locus_tag="VFV25_09820"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HEY1393663.1"
                     /translation="NHFWPILSALWGLDLLALPYAQRLDEVRARGLGIWDVYAHCRRE
                     GSLDSAIEDAVPNAIAGLKRLAPGLAGAAHNGGESARSMRITRELGLAVHRLPSTSPA
                     NASWSFERKLAAWREVFALYRLS"
     gene            375..1169
                     /locus_tag="VFV25_09825"
     CDS             375..1169
                     /locus_tag="VFV25_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HEY1393664.1"
                     /translation="MTTTTKKKEKPAPTLAPATVSEGGGIRYLHLGTPWVQGAMRVRK
                     PQAIELEYVQRMMVWMLLRPQERLGEGHAVQLGLGAAAITKFCHKVLRMRTTAVELNP
                     SVIGVCRLWFHLPPDDERLAVLNEDAARYAAAPEHIASADVLCVDLYDHEAASPVLDD
                     EAFYRNCHRLLADEGAMTVNLFGRDASFERSAARIARVFGADRVRVLKPTKEGNTIVL
                     AWKDMAWPERETLAARAENIDTRFKLPARKWLRLIQALPQPLPTTT"
     gene            1166..>2896
                     /locus_tag="VFV25_09830"
     CDS             1166..>2896
                     /locus_tag="VFV25_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEY1393665.1"
                     /translation="MTAAVPAQRSASAATKAHAFKGRLEWRILLRWLRDDGVIAAAEA
                     DRLAQRFAAGDSAQHPLVRIASVGLSHASSGAPLDVEALTEWLAKRAGLGYLRIDPLK
                     VDVARVADVMSITYAERRRALPVAVGATEVTIATCEPYDTDWLAEIEAHTKRRLKLML
                     ASPLELQRYTTEFYTLSRSVRAAQKSGQVSGPANFEQLVELGQSKQLDANDQGVVQVV
                     DWLWQYAFDQRASDIHLEPRRELGAIRFRIDGVLHTVYQLPLTVMGAMTARIKLLGRM
                     DVVEKRRPLDGRIKVRNPRGDEVEMRLSTLPTAFGEKMVMRIFDPDTAVKGLDALGFG
                     EHDGRRWEQLTAQPHGIILVTGPTGSGKTTTLYTTLKRLATDEVNVCTIEDPIEMIEP
                     AFNQTQVQPALELGFAEGLRALMRQDPDIIMVGEIRDLETAEMAIQAALTGHLVFSTL
                     HTNDAASAITRLTDIGVPPYLISATVIGVLAQRLVRTLCPACKQPDEGMTREILDEAI
                     KPWRMSGGLQPYKPVGCLECRMTGYRGRCGLYELMALSEALQPAIHPTPDVARLRQLA
                     VQHELRPLRLG"
BASE COUNT          478 a         1027 c          967 g          424 t
ORIGIN      
        1 aatcatttct ggcccatcct gtcggcgctg tggggcctgg acctgctcgc cttgccctat
       61 gcacagcgcc tggacgaagt gcgcgcacgc ggcctgggca tctgggatgt ctacgcgcat
      121 tgccggcgcg agggcagcct ggacagcgcg atcgaagacg ccgtgcccaa tgccatcgcc
      181 ggcctcaagc gcctggcgcc cgggctggcc ggcgcggcgc acaacggcgg cgaatcggcc
      241 cgcagcatgc gcatcacgcg cgagctgggg ctggccgtgc atcgcctgcc atcgaccagc
      301 ccggccaatg cctcatggag cttcgaacgc aagctcgcgg cctggcgcga ggtcttcgcc
      361 ctttaccgac tgtcatgaca actacgacca agaagaaaga gaaaccggcg ccgacgctgg
      421 cgccggccac cgtctccgag ggcggcggca tccgctacct gcacctgggt acgccttggg
      481 tgcagggcgc gatgcgggtg cgcaagcccc aggccatcga gctggaatac gtgcagcgca
      541 tgatggtgtg gatgctgctg cgtccgcagg agcgcctggg cgaagggcac gccgtccagc
      601 tgggcctggg cgcggctgcc atcaccaagt tctgccacaa ggtcttgcgc atgcgcacca
      661 cggcagtgga gctgaacccc agcgtgatcg gcgtgtgccg gctgtggttt cacctgccgc
      721 ccgacgacga gcgcctggcc gtgctcaacg aggatgccgc gcgctatgcc gccgcgcccg
      781 agcacatcgc atcggccgat gtcttgtgtg tcgacttgta cgaccacgag gccgcctcgc
      841 cggtgctgga cgacgaagcc ttctatcgca actgccaccg cctgctggct gacgaaggcg
      901 cgatgacggt gaacctcttc ggccgcgatg ccagcttcga gcgcagcgcc gcgcgcatcg
      961 ctagggtctt cggcgccgac cgcgtcagag tcctgaagcc gacgaaggag ggcaacacca
     1021 tcgtgctggc ctggaaggat atggcctggc ccgagcgcga aacgcttgcc gcgcgcgccg
     1081 aaaatatcga cactcgattc aagctgccgg cgcgcaagtg gctgcggctg atccaggcct
     1141 tgccccaacc cctgccgacc acgacatgac cgcagccgtg cccgctcagc gatctgcctc
     1201 cgccgccaca aaagctcacg ccttcaaggg ccgcctcgaa tggcgcatcc tgctgcgctg
     1261 gctgcgagat gacggcgtca tcgccgcggc cgaggccgac cgcctggccc agcgcttcgc
     1321 cgccggcgac agcgctcagc atccgctggt gcgcatcgcc tcggtggggc tgagccatgc
     1381 atccagcggc gcgcctctgg acgtcgaggc cttgaccgaa tggctggcca agagggcggg
     1441 cttgggctat ctgcgcatcg atccactcaa ggtcgacgtg gctcgcgtgg ccgacgtgat
     1501 gtcgatcacc tatgccgagc gccggcgcgc gctgccggtg gcggtgggtg cgaccgaggt
     1561 cacgatcgcg acctgcgagc cctacgacac cgactggctg gccgagatcg aggcgcacac
     1621 caagcgtcgc ctcaagctca tgctggccag ccccctggag ctgcagcgct acaccaccga
     1681 gttctacacg ctgtcgcgct cggtgcgcgc ggcgcagaag agcggccagg tctcggggcc
     1741 ggccaacttc gagcagctgg tggagctggg ccagagcaag cagctcgatg ccaacgacca
     1801 gggcgtggtg caggtcgtcg actggctgtg gcaatacgcc ttcgaccagc gcgcctcgga
     1861 catccacctg gagccgcgcc gcgagctggg cgcgatccgc ttccgcatcg acggcgtgct
     1921 gcacaccgtc taccagctgc cgctcaccgt gatgggcgcc atgaccgcgc gcatcaagct
     1981 gctgggccgc atggacgtgg tggaaaagcg ccgcccgctg gacgggcgca tcaaggtgcg
     2041 caacccgcgc ggcgacgagg tcgagatgcg cctgtcgaca ctgcctaccg ccttcggcga
     2101 gaagatggtg atgcgcatct tcgaccccga cacggcggtc aagggcctcg acgcgctggg
     2161 cttcggcgag catgacggcc ggcgctggga gcagctcacg gcccagcccc atggcatcat
     2221 cctcgtcacc ggccccaccg gctcgggcaa gaccaccacg ctctacacca cgctcaagcg
     2281 cctggcgacc gacgaggtga acgtctgcac catcgaagac ccgatcgaga tgatcgagcc
     2341 ggccttcaac cagacccagg tgcagccggc cctggagctg ggcttcgccg agggcctgcg
     2401 cgcgctgatg cgccaggacc cggacatcat catggtcggc gagatccgcg acctggaaac
     2461 cgccgagatg gcgatccagg ccgcgctcac cggccacctg gtcttcagca ctctgcacac
     2521 caacgacgcg gcctcggcga tcacgcgatt gaccgacatc ggtgtgccgc cttatctgat
     2581 cagcgcgacc gtgatcggcg tgctggccca gcgcctggtg cgcaccctgt gcccagcctg
     2641 caagcagccc gacgagggca tgacgcgcga gatcctggac gaggcgatca agccctggcg
     2701 catgagcggc ggcctccagc cctacaagcc ggtgggctgc ctcgaatgcc gcatgaccgg
     2761 ctatcgcggc cgctgcggcc tgtacgagct gatggcgctg agcgaggcct tgcagccggc
     2821 gatccacccg acgcccgatg tcgcgcggct gcgccagctc gcggtccagc acgagctgag
     2881 gccgctgcgc ctgggt
//
LOCUS       DASUCY010000497         2803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1569441, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000497 DASUCY010000000
VERSION     DASUCY010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1569441"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1277)
                     /locus_tag="VFV25_09835"
     CDS             complement(<1..1277)
                     /locus_tag="VFV25_09835"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEY1393666.1"
                     /translation="MHVSVAGGPPQVFGTVTAQLHALRDWLKAQGVRSVAMEATGVYW
                     LPLYGVLEAAGFEVRMVNGRQTRNLPGRKTDMQDCQWGATLHAHGLLRAGFVPPAQIR
                     RIQDYMRLRADHISMAATHVQHMQQALERMNIKLHDVIAKLTGVSGLAVVRAILGGER
                     DPQRLLALCDTQIQRAKAERIKESLRGTWTEEHLFALRQTLQSWEHYQAQIAECDRRV
                     EALLHDIDPGQPPNVSALAARPSRPDVNAPDIPNLRAILAQICGGQDLTQLPAHTEYG
                     VLQVIAEVGTDLTKWPSEKHFTAWMGLAPGSHQSGKRKRSAKRSRNRVGRLFCVIARS
                     LARSKDIALGGFYRRMAARRGGLVANIALARKLAALFWRVMVKGLDYVELGLKQYEAK
                     VLETKQRSLRRLARQLGQQLVPMTSAAPTKSPSP"
     gene            1702..2232
                     /locus_tag="VFV25_09840"
     CDS             1702..2232
                     /locus_tag="VFV25_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393667.1"
                     /translation="MKPFALFLSAAPLMLAACASTAPAQQQQPSTTSQVGQAVTTPLA
                     DLNLVKAEIPPVLIAAKKGPYGPPADRSCAALGAEIQALDAVLGADLDQPPTPTRPSL
                     IERGTDTAGDAAVGAVRGFAEGVVPFRGWVRKLTGAERYSKEVSAAIAAGGIRRAFLK
                     GLGAAAGCPAPAAPRA"
     gene            complement(2327..>2803)
                     /locus_tag="VFV25_09845"
     CDS             complement(2327..>2803)
                     /locus_tag="VFV25_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEY1393668.1"
                     /translation="LMAASPRPIRFIMDHRIFKLPLLGTLFRLAKAIPIAPQKEDAQA
                     YERAFDEARRVLGEGELLCIFPEGGITRDGQLQPFRGGIMKILETHPVPVVPLALQNL
                     WGSYFSRIEGGKAMVKPWRRGWFSRVGLSAGEAMAPDAVTPEALHERVGALLAQPE"
BASE COUNT          464 a          938 c          927 g          474 t
ORIGIN      
        1 ggcgatgggc tcttcgttgg cgctgcgctc gtcatcggca cgagttgctg accgagttgt
       61 cgcgcgagtc ggcgcagaga tcgctgcttg gtctcgagca ccttggcttc gtattgcttc
      121 agaccgagct cgacatagtc caggcccttg accatgacgc gccagaacag ggcggcgagc
      181 ttgcgcgcca gcgcgatgtt ggcgaccagg ccgccgcgac gcgcggccat gcgcctgtag
      241 aagccgccca gggcgatgtc cttgctgcgc gccaggcttc gcgcgatcac gcagaagagc
      301 cgtccgacgc ggttgcggct tcgcttggcc gagcgcttgc gcttgccgct ttgatggctg
      361 ccgggcgcga gtcccatcca ggccgtgaag tgtttttcgc tcggccactt ggtcagatcg
      421 gtgccgactt cggcgatgac ttgcaagacg ccgtattcgg tgtgcgccgg caactgcgtg
      481 agatcttgtc caccgcagat ctgcgcgagg atggcgcgca ggttggggat gtccggcgca
      541 ttgacgtcgg gcctgctggg acgggcggcc aaggcgctca cgttcggcgg ctgtccaggg
      601 tcgatgtcgt gcagcagcgc ttcgacgcgg cggtcgcact cggcgatctg ggcttggtag
      661 tgctcccagc tttgcagcgt ctgcctgagc gcgaacagat gctcctcggt ccaggtgccg
      721 cgcaaggact ccttgatgcg ctcggccttg gcgcgttgga tctgggtgtc gcacagcgcc
      781 agcaggcgct gcggatcgcg ctcgccgccg aggatggcac gcaccacggc caaaccgctg
      841 acaccggtca acttggcgat cacgtcatgc agcttgatat tcatgcgctc cagcgcctgc
      901 tgcatgtgct gcacgtgggt ggcggccatg ctgatgtggt cggcgcgcag gcgcatgtaa
      961 tcctggatcc ggcggatctg cgcgggaggc acaaagcccg cacgcagcag accgtgcgcg
     1021 tgcagcgtcg caccccactg gcagtcctgc atgtcggtct tgcgcccggg cagattgcgc
     1081 gtctgacggc cgttgaccat gcgcacttcg aacccggccg cctcgagcac gccgtacagc
     1141 ggcagccagt acaccccggt ggcctccatc gccaccgagc gcacgccttg tgccttgagc
     1201 cagtcgcgca gcgcgtgcaa ctgcgcggtg acggtgccga acacctgggg cgggccgccc
     1261 gcgacggata catgcatttg ctcgctgcca acgtcaacga acgcggcgtg cgcatcaaga
     1321 acaggcaaat cgctcatgtc gatgctcccg atcgagtcac caagatcgcg aggcctcgcc
     1381 gcaagcccgg tcgcgttcag ggcatgacag atctttccaa cgggaagcga cgctcaccaa
     1441 aatgtgcgcc agcccgcgac cggagccaat ctacataccg ggcagtcgga cgcaccaaag
     1501 gactgagcgg ccacgctgct cgcggcaaga ctccgcatcc gccacagtag ccggttcaag
     1561 gtccgtgtgc ggtttcgacg ggaacagcag gctacatagc atctttctgg cagcgagttc
     1621 atatcgtcga ttgaccggtt ggggtcgtgt gtcgccggtc ggccctgagc gagtcgcacg
     1681 gctgctgcta acctcgcggc gatgaagccc tttgctctgt ttctctccgc ggcaccgctg
     1741 atgcttgccg cctgcgcctc caccgcaccc gctcaacaac aacagcccag cactacaagc
     1801 caggtcggcc aggcggtgac gacgccgctg gccgatctca atctcgtgaa ggccgagatt
     1861 ccgccggtgc tgatcgccgc gaagaaaggg ccctacggtc caccggccga tcgcagctgc
     1921 gcggccctgg gcgccgaaat ccaggcgctg gacgccgtgt tgggtgcgga cttggaccag
     1981 ccgcccacgc cgacccgccc gagcctgatc gagcgcggca ccgacacggc aggcgacgcg
     2041 gcggtcggcg ccgttcgcgg ctttgctgaa ggcgtggtgc ccttcagggg ctgggtgcgc
     2101 aaactgacgg gcgccgagcg ctactcaaag gaagtgtccg cagccatcgc cgccggcggc
     2161 atacgccgcg ccttcctgaa ggggctcggt gcggcggccg gctgcccggc gccggccgcg
     2221 ccgcgtgctt gatcatcaag atgctacaga atttgttcca atcttcattg aaatacgacg
     2281 ggtagatcac aagaaaatta gaaatcaacg ggcaaagggc gcgccctcac tcaggctgcg
     2341 ccagcagcgc cccgacacgc tcgtgcaagg cctcgggcgt caccgcatcg ggcgccatcg
     2401 cctcgccggc actcagaccc acacggctga accagccgcg gcgccagggc ttcaccatcg
     2461 ccttgccgcc ttcgatgcgc gagaagtacg agccccagag gttctgcagc gcgagcggca
     2521 ccaccggcac cgggtgtgtc tcgaggatct tcatgatgcc gcccctgaag ggctgcagct
     2581 gcccgtcgcg cgtgatgccg ccttcgggaa agatgcagag cagctcgccc tcgccgagca
     2641 cgcggcgcgc ttcatcgaag gcgcgttcgt aggcctgcgc gtcctccttc tgcggggcga
     2701 tcgggatggc cttggccaaa cggaacagcg tgcccaggag cggcagcttg aagatgcggt
     2761 gatccatgat gaagcggatc ggccgcggac tcgcggccat cag
//
LOCUS       DASUCY010000498         2549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1360631, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000498 DASUCY010000000
VERSION     DASUCY010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1360631"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="VFV25_09850"
     CDS             <1..419
                     /locus_tag="VFV25_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEY1393669.1"
                     /translation="LVSAPNLFWSVGSTLAESIFDGGQRRLASAQARASAEQATANYR
                     QAVLVALQEVEDNLELASQLQRETGYQQDALKAAQRNLEIVLDQYRAGTVSYLNVVIA
                     QNAAYTSENSLLSARNRQLAAVNTLLKNIAGRWEKA"
     gene            complement(416..1168)
                     /gene="uppS"
                     /locus_tag="VFV25_09855"
     CDS             complement(416..1168)
                     /gene="uppS"
                     /locus_tag="VFV25_09855"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008350084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEY1393670.1"
                     /translation="MSEFASSTLAVPQASRIPRHIAIIMDGNGRWAKQRGLPRLAGHE
                     RGGEAVRRTVRACLDLGIEYLTLFAFSTENWRRPPAEVSWLQRLFMQLLRREVNALHE
                     HGVRLVVAGDIARFDAALVEAIRAGESLTRDNRRLTLTIAASYGGRWDILQATHRLLE
                     DRPGIATQFDEKDLAPYLAMQHAPEPDLFIRTGGEQRVSNFLLWQLAYTELYFTDTLW
                     PDFDAEALQRAIGWYGECERRFGRTSEQLSSP"
     gene            complement(1220..>2549)
                     /locus_tag="VFV25_09860"
     CDS             complement(1220..>2549)
                     /locus_tag="VFV25_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HEY1393671.1"
                     /translation="VGTHKLLSAETKFKNLGLLVIDEEHRFGVRHKEAMKALRAEVDV
                     LTLTATPIPRTLGMALEGLRDLSVIATAPQRRLAIKTFVRNEGHGTIREAVLRELKRG
                     GQVYFLHNEVETIENRRRKLEELLPEARIAVAHGQMPERELERVMREFVGQKHNLLLC
                     STIIETGIDVPTANTIVMSRADKFGLAQLHQLRGRVGRSHHQAYAYLMVPDVEGLTKQ
                     AAQRLEAIQNMEELGSGFYLARHDLEIRGAGEVLGENQSGNMMEVGFQLYNEMLAEAV
                     RSLKAGREPDLLSPLSATTEINLHAPALLPDSYCGDVHTRLSLYKRLATADKLDKIDA
                     MLEEIVDRFGKLPAQAQTLFDTHRLRVQAKPYGVVKIDAAPHVMNISFRPKAPIDPLR
                     IIELVQKNRHIKLAGNDKLRVEKSMPEPKDRAQFIRDVLRSLGTPVREAA"
BASE COUNT          392 a          849 c          868 g          440 t
ORIGIN      
        1 acctggtgag cgcgcccaac ctgttctggt ccgtgggctc gacgctggcc gaatcgatct
       61 tcgacggcgg ccagcgccgg ctcgccagcg cccaggcgcg cgccagcgcc gagcaggcca
      121 cggccaacta ccgccaggca gtgctggtcg cgctgcagga agtggaagac aacctcgagc
      181 tcgccagcca gctgcagcgc gaaacgggct atcagcagga cgccctgaag gccgcgcaac
      241 gcaacctgga gatcgtgctg gaccagtacc gcgccggcac ggtgagctat ctcaacgtcg
      301 tgatcgcgca gaacgcggcc tataccagcg agaacagcct gctgagcgcg cgcaatcgcc
      361 agttggcggc ggtcaacacg ctgctcaaga acatcgcggg gcggtgggag aaggcctagg
      421 gcgaactcag ctgctcgctg gttcggccga agcgccgttc gcactcgccg taccaaccga
      481 tggcgcgctg cagcgcctcg gcgtcgaaat ccggccatag cgtatcggtg aaatacagct
      541 ccgtgtaggc gagctgccat aagaggaagt tgctcacccg ctgctcgccg ccggtgcgga
      601 tgaacaggtc cggctcgggt gcatgctgca tcgccagata gggcgcgaga tccttttcgt
      661 cgaactgcgt cgcgatccct ggtcggtctt cgagcaagcg gtgcgttgcc tgcaggatgt
      721 cccagcgacc cccgtagctg gctgcgatgg tcagcgtcaa gcgccggttg tcgcgggtca
      781 gcgactcgcc ggcccggatc gcctccacta gcgcggcatc gaagcgcgcg atgtcccccg
      841 ccaccacgag gcgcacgcca tgttcgtgca aggcgttgac ctcacgccgc agcagttgca
      901 tgaagagccg ctgcagccag ctcacctccg caggcggccg gcgccagttc tcggtgctga
      961 aggcgaacag cgtcaggtac tcgataccca ggtccagaca agcccgcacg gtgcgccgga
     1021 ccgcctcgcc cccgcgctcg tggccggcca gccgaggcag gccgcgctgc ttcgcccaac
     1081 gcccgttgcc gtccatgatg atcgcgatgt gccgcgggat ccggctggcc tgcggcaccg
     1141 cgagcgtgga gctggcgaac tctgacatgg cgagggttcg atcggcgcgg ttgacaaacg
     1201 ttcggccgca ggaggcggct caggcggcct cccgcaccgg tgttcccagc gagcgcagca
     1261 cgtcgcgtat gaactgagcc cggtccttgg gctcaggcat cgatttctcg acgcgcagct
     1321 tgtcgttgcc tgccagcttg atgtggcggt tcttctgcac cagttcgatg atgcgcagcg
     1381 ggtcgatcgg cgccttgggc cggaagctga tgttcatcac atgcggcgcc gcgtcgatct
     1441 tgaccacgcc gtagggcttg gcctgcacgc gcaggcggtg ggtgtcgaac agcgtctgcg
     1501 cctgggctgg gagcttgccg aagcggtcga cgatctcttc gagcatcgca tcgatcttgt
     1561 cgagcttgtc ggcggtggcc agccgcttgt agagcgacag ccgcgtgtgc acgtcgccac
     1621 agtagctgtc gggcagcagg gcgggggcat gcaggttgat ctcggtggtc gcggacagcg
     1681 gtgacagcag atcgggctcg cggccggcct tgagcgagcg caccgcctcg gcgagcattt
     1741 cgttgtagag ctggaagccc acttccatca tgttgccgct ctggttctcg cccagcacct
     1801 cgccggcgcc acggatctcc aggtcgtgcc tcgcgaggta gaagcccgag cccagctcct
     1861 ccatgttctg gatcgcttcc agcctttgcg ccgcctgctt ggtgaggcct tcgacatcgg
     1921 gcaccatcag gtaggcatag gcttggtggt gcgagcggcc gacacgcccg cgcagctggt
     1981 gcagctgggc caggccgaac ttgtcggcgc ggctcatcac gatggtgttg gccgtgggca
     2041 cgtcgatgcc tgtctcgatg atggtggagc acagcagcag gttgtgcttc tggccgacga
     2101 actcgcgcat cacgcgctcc agctcccgct cgggcatctg gccgtgcgcc acggcgatgc
     2161 gcgcctcggg caacagctct tcgagcttgc ggcgccggtt ctcgatggtc tcgacctcgt
     2221 tgtgcaggaa gtagacctgc ccgccgcgct tgagctcgcg cagcaccgcc tcgcggatgg
     2281 tgccatgccc ctcgttgcgc acgaaggtct tgatggccag gcggcgctgc ggcgcggtcg
     2341 cgatgaccga gaggtcgcgc aggccttcga gcgccatgcc cagcgtgcgc gggatcggcg
     2401 tggcggtgag cgtcagcacg tccacctcgg cgcgcagcgc cttcatcgcc tccttgtggc
     2461 gcacgccgaa gcgatgctcc tcgtcgatga cgagcaggcc gaggttcttg aacttggtct
     2521 cggcgctcag cagcttgtgc gtgccgacc
//
LOCUS       DASUCY010000499         2951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_894765, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000499 DASUCY010000000
VERSION     DASUCY010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_894765"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2905)
                     /locus_tag="VFV25_09865"
     CDS             complement(<1..2905)
                     /locus_tag="VFV25_09865"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HEY1393672.1"
                     /translation="MPPVSKPRQLLVQATYADPNGEVQTLSQTVPIWPSAVVLGLRTD
                     AWVSVKQEMAAQVIVLDTAGKPVPNVSVKVRAVAHHTQSVRRRLVGGFYAYDNQNADE
                     ELGQVCSGTSDARGIVLCDVSLKSPGEIELIAQAKDAGDHLAQAASSVWVTKQGEVWF
                     GGDNDDRMDVIPERRSYEPGEIVKLQVRSPFRHATALVSIERNGIIETRNVEMHGRDP
                     TIEVPVKAEWAPNVYISVLAVRGRVHEVPWYSFFTWGWRSPGEWWRAHRDEGGFYQAP
                     TAMVDLSKPAFKLGIAEIEVGTAAHQLKITVQPDKASYPIRATSEVRVKVTLPDGSPA
                     PQGTEVALAAVDEALLELMPNTSWDLLPAMIRKREFGIETSTAQMQIIGKRHYGKKAV
                     AAGGGGGQFPTRELLDTLLLWNPRVVLDAKGEALVKLPLNDSLTSFRIVAVADAVRGP
                     NAAWFGTGSASIRATQDLQLISGLPPLVREADHYRAMFTLRNTTAHAVDATLNATVGG
                     TKLPEQKLHIDANGAAESGWDVDVPYNASTLDWLIAADSAAAHDRLKLQQQVREAVPV
                     RVQQATLMQADKLTAIPIAPPASAVADAQGALRGGIEVALKPRLGDGLPGVREYFLSY
                     PWICLEQQASIAIGLRDADRWRRVAEQLPLYLDSDGLANYFPPLPGEAARGSDTLTAY
                     LLAISDEAGRLGYDFAIPTDTRDKMERGLIGFVEGHLQRDFWRPAFLRNGDLDVRKLA
                     AIEALSRSGKARAKLLDSVQILPNQWPTGAVIDWLSILDRMKDIPDRDKRIAEAEQIL
                     RARLNVQGTRLGFSTERDDSWWWLMVNGDVNSTRMILAVLNNVAWKDDMPRLVTGTLQ
                     RMQFGRWTTTTANAWGSVAMEAFSKRFEPEPVTGTTRASFEPGGPARSLDWAKSPAGG
                     VLAMGWPTGFAVGGNKSDTSVKLAHEGGGKPWLTFTSKAAVPITQAFSSGYRIA"
BASE COUNT          426 a          996 c          998 g          531 t
ORIGIN      
        1 tggcgatgcg atagccgctg ctgaaagctt gagtgatggg caccgcggcc ttgctcgtga
       61 acgtgagcca gggcttgccg ccgccttcat gggcgagctt cacgctcgtg tcggacttgt
      121 tgcccccgac tgcgaagccg gtcggccagc ccatggcgag cacaccgccg gcgggcgatt
      181 tcgcccagtc cagcgatcgc gcggggccgc cgggctcgaa gctggcgcgc gtggtgccgg
      241 tgaccggctc cggctcgaag cgcttggaga aggcctccat cgcgaccgag ccccaggcgt
      301 tggcggtcgt ggtcgtccac cgcccgaact gcatgcgctg cagcgtgccg gtcaccagcc
      361 ggggcatgtc gtccttccag gccacgttgt tcagcaccgc gaggatcatc cgcgtgctgt
      421 tgacatcgcc gttgaccatc agccaccacc agctgtcgtc gcgctcggtc gagaagccca
      481 ggcgcgtgcc ctgcacgttg agccgcgcgc gcaggatctg ctcggcctcg gcgatacgct
      541 tgtcgcgatc ggggatgtcc ttcatgcgat cgaggatgga aagccaatcg atcaccgcgc
      601 cggtcggcca ttggttcggc aggatctgca ccgaatcgag cagcttcgcc cttgccttgc
      661 cgctgcgtga caaggcctcg atggcggcca gcttgcgcac gtcgaggtcg ccgttgcgca
      721 ggaaggccgg gcgccagaag tcgcgctgca agtggccctc gacaaagccg atcagcccgc
      781 gctccatctt gtcgcgtgtg tccgttggga tcgcgaagtc atagccgagc cgcccggctt
      841 cgtcgctgat cgccagcagg taggccgtca gcgtatcgct gccgcgcgca gcttcacctg
      901 ggagcggcgg gaagtagttg gccagcccgt cgctgtccag gtacagcggg agctgctccg
      961 cgacgcgccg ccagcggtcg gcatcgcgca ggccgatcgc gatggaggct tgctgctcca
     1021 ggcagatcca cgggtagctc aggaaatatt cgcgcacccc gggcaggccg tcgccgagcc
     1081 tgggcttcag cgctacctcg atgcctccgc gcaaggcccc ttgcgcgtct gccacggcac
     1141 tggcaggcgg cgcgatgggg atggcggtca acttgtccgc ttgcatcagc gtggcttgct
     1201 gcacgcgcac cggcaccgct tcgcgcacct gctgctgcag cttcaggcgg tcgtgggcgg
     1261 cggcagagtc agccgcgatc agccaatcga gcgtgctcgc gttgtacggc acgtcgacat
     1321 cccatcctga ctcggcggcg ccgtttgcat cgatgtgcag cttttgctcg ggcagtttcg
     1381 tgccgcccac cgtcgcgttc aaggtcgcgt cgaccgcgtg cgccgtggtg ttgcgcagcg
     1441 tgaacatcgc gcgatagtgg tcggcttcgc gaaccagcgg cggcaggccc gagatgagct
     1501 gcaagtcctg cgtcgcgcgg atgctggcgc tgcccgtgcc gaaccaggcg gcattggggc
     1561 cccgtacggc gtcggccacc gcgacgatgc ggaaggacgt cagcgaatcg ttgagcggca
     1621 gcttgaccag cgcctcgccc ttggcgtcga gcacgaccct tgggttccac agcagcagtg
     1681 tgtccagcag ctcccgggtc ggaaattgcc cgccgcctcc accggccgca acggccttct
     1741 tgccgtagtg gcgcttgccg atgatctgca tctgcgcggt cgaagtctcg atgccgaatt
     1801 cgcgtttgcg gatcatggcc ggcagcaggt cccagctggt gttgggcatc agctccaaaa
     1861 gcgcttcatc gacggcagcg agcgcgacct cggtgccctg gggtgcgggt gagccgtcgg
     1921 gcagtgtcac cttcacgcgc acctcgctgg tggcgcgaat cgggtagctg gccttgtccg
     1981 gctggacggt gatcttcagc tgatgcgctg cggtaccgac ttcgatctcg gcgatgccga
     2041 gtttgaaggc cggtttcgac aagtcgacca tcgcggtcgg cgcctggtag aagccgcctt
     2101 cgtcgcggtg ggcccgccac cattcgccgg gcgaacgcca gccccaggtg aagaacgaat
     2161 accacgggac ctcgtgcacg cgcccgcgca ccgcgagcac cgaaatgtag acgttcggcg
     2221 cccattcggc cttgaccggg acttctatcg tcggatcgcg cccgtgcatc tcgacgttgc
     2281 gcgtctcgat gatgccgttg cgctcgatcg agaccagcgc cgtggcatgg cggaaggggc
     2341 tgcgcacctg cagcttcacg atttcgcccg gctcgtatga ccgccgctcg gggatcacgt
     2401 ccatgcggtc gtcgttgtcg ccgccgaacc aaacctcgcc ttgcttcgtg acccacaccg
     2461 acgaggccgc ctgcgcgagg tggtccccgg catccttggc ctgcgcgatg agttcgatct
     2521 cgccggggga tttcaggctc acgtcgcaca gtacgatgcc gcgcgcatcg ctggtgccgc
     2581 tgcagacctg gccgagctcc tcgtcggcgt tctggttgtc gtaggcatag aagccgccga
     2641 ctaggcggcg ccgcacggat tgcgtgtggt gggcaacggc gcgcactttc acgctgacgt
     2701 tcggcactgg cttgccggcc gtgtccagca cgatgacctg cgcggccatt tcctgcttga
     2761 ccgacaccca agcatccgtg cgcagcccca gcaccacggc cgagggccat atcggcacgg
     2821 tctggctcag cgtctgcact tcgccgttgg ggtcggcata ggtcgcctgc accagcagct
     2881 ggcgcggttt gctgacgggt ggcatggaag gcagcgtgac ctgcgccgcg ccctgcttgt
     2941 cgagcgtcgc g
//
LOCUS       DASUCY010000500         2280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_975236, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000500 DASUCY010000000
VERSION     DASUCY010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_975236"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            463..1395
                     /locus_tag="VFV25_09870"
     CDS             463..1395
                     /locus_tag="VFV25_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triacylglycerol lipase"
                     /protein_id="HEY1393673.1"
                     /translation="MKRFASVLLGALALCGALLASPAAKADTYTQTQYPIVLVHGLLG
                     FDAIGPVNYWYGIPPALSSGGAAVYVSQESAVNSSVVRGEQLLQELLQLQAAYGYTKF
                     NLIGHSHGGQTVRYVASVRPDLVASVTTVGTPHQGSPVADAIEAGTSATGTTAAVAAV
                     VNALGNFISWVSGGGSLPQDAMGALTSLDTAGAQAFNASYPAGAPTSPCGQGPASVNG
                     VAYYSAGGTSVLTNVFDLSDPLLGLTSLFFGFQSNDGLVGQCSSHWGTVLRDDYPWNH
                     LDEVNQSFGLIGWFAPDPVAFYRAQANRLKLQGL"
     gene            1400..2263
                     /locus_tag="VFV25_09875"
     CDS             1400..2263
                     /locus_tag="VFV25_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase chaperone"
                     /protein_id="HEY1393674.1"
                     /translation="MPRRRSLLLIGSGAAAALAVLLWGLSCGSEPAQAVAHDAAPAVR
                     AALPAAQAALAADRHDPVVFERWLQQKSSLRGTELDGAWGELDVHGRLKPALALRRRF
                     DQLLTLQGEAGLDEIAGYIAHEAGDLLGADGGQQVVAVWQHYLQVKQYRYSTRVDLQD
                     RSSWATALAERQSVRASLLGADWAQAFYAEEDALMQAALQADAQQQQPLIDRSRLDPV
                     ALQRLREADQAWSRWQARLAEARQHWAELQADAGTSPPQRQAAMEQWMQPRFDAGEQR
                     RLRALLQLPGA"
BASE COUNT          349 a          797 c          797 g          337 t
ORIGIN      
        1 tgctgctggc gcggcggcgg ttgacctagg aggccgcgca tagcggcgcg ctacctcggc
       61 gtgccgtcga gttgcccgcc tcttgttgaa gcccggcggc cgagcagcag cggtgcaccc
      121 tggtggtggc agccgcggcc aagttgttga ccaggcgagc caaggcgtgg tcaagggcgg
      181 attgctgcat cgcagctttg ggaacaccct gatcccataa aaaggaacgc tcgttttaat
      241 gcgcctcgca tgaggctttt gcgaagacct cgcagggcct cacgccagtg ggcgagcacg
      301 tgggccatcc cgtccctcgg agcgaggccg cgcaacggcg gcaacaaggc cgctggctct
      361 ctaggctctc caatccgcag cggccacggc agttcttcag gcagtaagta agcaaaaggc
      421 cgctccttct tcagatcgac acaacgaaag aggagaaaag caatgaagcg cttcgcttcg
      481 gtcctgctgg gcgcgctcgc cttgtgcggc gccctgctgg ccagcccggc cgccaaggcc
      541 gacacctata cccagacgca atatcccatc gtgctggtgc acggcctgct cggcttcgat
      601 gccatcgggc cggtcaacta ctggtacggc ataccgccgg cgctgagcag cggcggcgcg
      661 gcggtctatg tgagccagga atcggccgtc aacagcagcg tcgtgcgcgg cgagcagctg
      721 ctgcaggagc tgctgcagct gcaggcggct tatggctaca ccaagttcaa cctgatcggc
      781 cacagccatg gcggccagac cgtgcgctac gtggcgagcg tgcggcccga cctggtggcc
      841 tcggtcacga cggtgggcac gccgcatcag ggcagcccgg tggccgatgc gatcgaggct
      901 ggcaccagcg cgaccggcac caccgcggcg gtcgcggccg tcgtcaatgc cctgggcaac
      961 ttcatctcct gggtctccgg cggcggcagc ctgccgcagg acgcgatggg cgcactgacc
     1021 tcgctcgaca cggccggcgc gcaagccttc aacgcgagct atccggccgg cgcgccgacc
     1081 agcccctgcg gccagggccc agccagcgtg aacggcgtcg cctactactc cgccgggggc
     1141 acctcggtgc tgaccaatgt cttcgacctg tccgatccgc tgctgggcct cacttcgctc
     1201 ttcttcggct tccagagcaa cgacggcctg gtcggccaat gctcctcgca ctggggcacg
     1261 gtgctgcgcg acgactatcc ctggaaccac ctcgacgaag tgaaccagag cttcggcctg
     1321 atcggctggt tcgcgccgga cccggtggcc ttctaccgcg cccaggccaa ccggctgaag
     1381 ctgcaagggc tgtgaggcga tgccgcgccg gcgaagcctg ctgttgatcg gcagcggcgc
     1441 ggccgccgcg ctggcggtgc tgctatgggg cttgtcctgc ggcagcgaac cagcgcaggc
     1501 cgttgctcac gacgcggcgc ccgctgtgcg cgcggccctg cccgccgcac aggccgcact
     1561 cgcggccgac cgtcacgacc cggtggtctt cgagcgctgg ctgcagcaga agagctcgct
     1621 gcgcggcacc gagctcgacg gcgcctgggg cgagctcgat gtccacgggc ggctcaagcc
     1681 tgcgctggcg ctgaggcggc gcttcgacca gctgctgacg ctgcagggcg aggccggtct
     1741 cgacgagatc gccggctaca tcgcgcatga ggccggcgac ctgctcggcg ccgatggcgg
     1801 gcagcaggtg gtggcggtgt ggcagcacta cctgcaggtc aagcaatacc gctacagcac
     1861 ccgcgtcgac ctgcaggacc gcagcagctg ggccacggcg ctggccgagc gccaatcggt
     1921 gcgcgccagc cttctgggcg cggactgggc ccaggccttc tatgccgagg aggacgcgtt
     1981 gatgcaggcg gcgctgcagg ccgatgcgca acagcagcag ccgctgatcg accgctcccg
     2041 cctcgacccc gtcgcgctgc agcgtctgcg cgaggccgac caggcctgga gccgctggca
     2101 ggcccgcctg gccgaggcgc gccagcattg ggccgagctg caggccgacg ccggcacttc
     2161 gccgccgcag cgccaggccg cgatggagca atggatgcag ccgcgcttcg atgcgggcga
     2221 gcagcgccgt ctgcgcgcgc tgctgcagct gcctggggcc tgagcgggga gcgcagttct
//
LOCUS       DASUCY010000501         2204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_429218, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000501 DASUCY010000000
VERSION     DASUCY010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429218"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="VFV25_09880"
     CDS             complement(<1..1001)
                     /locus_tag="VFV25_09880"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEY1393675.1"
                     /translation="MDLLMSLRDGLVAWLAHGLLDAAWWQVVLYTLAFTHVTIAAVTI
                     FLHRSQAHRALDLGPIPSHFFRFWLWTGTGMVTKEWVAIHRKHHAKCETEDDPHSPQT
                     RGIKTVLLQGSELYRAEAKNQETLAKFGHGTPVDWMERNVYSRYTWQGVGLTLILDLL
                     LFGAIGAAVWAVQMLWIPITAAGIINGLGHWWGYRNFEAPDASTNVSPWGIIIGGEEL
                     HNNHHTYPTSAKLSVKKGEFDIGWVYIRGLEMLGWAKVRKIAPELKLGAVKPVADSKT
                     LEAVIANRYEVMAKYAKELKRACGAELDRLKAQGAKNGAKWAEMRLARRWLHRDDDK"
     gene            complement(1152..>2204)
                     /locus_tag="VFV25_09885"
     CDS             complement(1152..>2204)
                     /locus_tag="VFV25_09885"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEY1393676.1"
                     /translation="GAMERRLAILGWQGSDTFLRGALGPQEIEWAGRARGVDVKTLPE
                     KLRHNLPDWLAAPLQASLGEEFWALVSSLNAPAPLDLRVNVLKAKREEVLAALQAEGI
                     AAQPTPYSPWGLRVDGKPALNKLDVFTRGEVEVQDEGSQLLALLTDAKRGEMVVDFCA
                     GAGGKTLALGAMMRNTGRLYAFDISGHRLDALKPRLARSGLSNVYPAQIAHERDERIK
                     RLAGKIDRVLVDAPCSGLGTLRRNPDLKWRQSPQAIAELQAKQTAILGSAARLLKPGG
                     RLVYATCSLLEAENEQIAAAFSESHADFAVRPAQELLEQAKLARAGELVAGPYLRLWP
                     HRHQTDGFFAAVWERK"
BASE COUNT          358 a          734 c          710 g          402 t
ORIGIN      
        1 atcttgtcgt cgtcgcggtg cagccagcgg cgcgccagcc gcatctcggc ccatttggcg
       61 ccgttcttgg cgccctgagc cttcaggcga tcgagctcgg cgccgcaggc acgcttgagc
      121 tccttggcgt acttggccat cacctcgtag cggttggcga tgacggcttc cagcgtcttg
      181 gaatcggcca ccggcttcac ggcgcccaac ttgagctccg gcgcgatctt gcgcaccttg
      241 gcccagccca gcatctcgag gccgcggata tagacccagc cgatgtcgaa ctcgcctttc
      301 ttgaccgaca gcttggcgct ggttggatag gtgtggtgat tgttgtgcag ttcctcgccg
      361 ccgatgatga tgccccaggg cgagacattg gtcgacgcgt cgggcgcctc gaaattgcgg
      421 tagccccacc agtggccgag gccattgatg atgccggccg cggtgatcgg gatccacagc
      481 atctgcactg cccacacggc cgcgccgatg gcgccgaaca gcagcaggtc gaggatcagc
      541 gtcaggccca cgccctgcca ggtgtagcgg ctgtagacgt tgcgctccat ccagtcgacc
      601 ggcgtgccgt ggccgaactt ggccagcgtt tcctggttct tggcctcggc gcgatagagc
      661 tcgctgcctt gcagcagcac ggtcttgatg ccgcgcgtct gcgggctgtg cgggtcgtct
      721 tcggtctcgc acttggcgtg gtgcttgcgg tggatggcga cccattcctt ggtcaccatg
      781 ccggtgcctg tccacagcca gaagcggaag aaatgcgagg ggatcggccc caggtcgagg
      841 gcacgatgcg cctgcgagcg gtgcaggaag atcgtcaccg cggcaatcgt cacatgggtg
      901 aaggccagcg tgtagagcac gacctgccac cacgcggcgt cgagcaggcc atgggccaac
      961 caggcaacca gcccatcccg caacgacatc agtaaatcca ttcttgtgtc ctcttggcta
     1021 agcgcgtgat tttatgaggt gcagactatt aacgcacgat gaaagttcgc cgcatacgct
     1081 cattcgtgcc caagaatgcg gcaatttgcg gtcgatcaag caatgtggtc aagcctatcg
     1141 ataggcgctt gctattttct ttcccagacc gcagcaaaaa agccgtccgt ctggtgccgg
     1201 tgtggccata gccgcagata gggcccggcc accagttcgc cggcgcgcgc cagcttggcc
     1261 tgttccagca gctcttgcgc cggccgcacc gcgaaatcgg catgcgattc gctgaaagca
     1321 gctgcgatct gctcgttttc ggcttccagc aggctgcagg tggcatagac cagccgcccg
     1381 cccggtttga gcagacgggc ggcgctgccc aggatggcgg tctgcttggc ctgcagttcg
     1441 gcgatggcct gcggcgactg acgccacttg aggtcaggat tgcggcgcag cgtgcccagg
     1501 cccgagcaag gcgcatcgac cagcacacgg tcgatcttgc cggccagacg cttgatgcgc
     1561 tcgtcgcgct cgtgcgcgat ctgcgcaggg tagacattgc tcagcccgct gcgcgccagg
     1621 cgcggcttca gcgcatcgag gcggtggcct gaaatgtcaa aggcgtagag ccggcccgta
     1681 ttgcgcatca tcgcgcccag ggccagggtc ttgccgccgg cgccggcgca gaagtccacc
     1741 accatctcgc cgcgcttcgc atcggtcagc agggccagca actggctgcc ttcgtcctgc
     1801 acctcgacct caccgcgcgt gaagacatcg agcttgttca gcgccggctt gccgtctacg
     1861 cgcaggcccc agggcgagta aggcgtcggc tgggccgcga taccctcggc ctgcagcgcg
     1921 gccagcacct cttcgcgctt ggccttcagc acattgacgc gcaggtccag aggtgccggc
     1981 gcattgaggc tcgacaccag cgcccagaat tcctcgccca ggctcgcctg cagcggcgcg
     2041 gccagccagt cgggcaggtt gtgacgcagc ttctcgggca gggtcttgac gtcgacgccg
     2101 cgcgcgcggc cggcccattc gatctcttgc gggcccaggg cgccacgcaa aaaggtatcg
     2161 ctgccctgcc agccgagaat ggccaggcgc cgctccatcg cacc
//
LOCUS       DASUCY010000502         3804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1441193, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000502 DASUCY010000000
VERSION     DASUCY010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1441193"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /gene="pheA"
                     /locus_tag="VFV25_09890"
     CDS             complement(<1..879)
                     /gene="pheA"
                     /locus_tag="VFV25_09890"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEY1393677.1"
                     /translation="MTAPPPPSVSDPTGSLADLRTQIDEVDQQLLTLLNRRAHIAELV
                     GEVKRREGTPFFRPDRVAQVIEKIERANAGPLKGPHVAAIWREIMSACLALESPQRVA
                     VLGPEGTFCEQAAIEYFGRAADLIYCASFDEVFHATTSGSAQYGVVGVENSNEGAVTR
                     SLDMFLQSPVHVVGEVSLLVRHNLLRKENSLEGIEAVLAHPQALAQCQTWLAKHLPRA
                     ERRAVSSNAEGARLASTNPAWAGLASERAATQYGLHIVAHAIQDDAYNRTRFAVICLP
                     QTLDMPPPSGRDCTSLV"
     gene            complement(885..1976)
                     /gene="serC"
                     /locus_tag="VFV25_09895"
     CDS             complement(885..1976)
                     /gene="serC"
                     /locus_tag="VFV25_09895"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HEY1393678.1"
                     /translation="MTRPYNFSAGPAVLPEPVLSQAAAEMLDWQGSGMSVMEMSHRGR
                     EFISIYEQAQADLRELLAVPEDFRILFMQGGGIAENAIVPLNLSRGGAADFVVTGSWS
                     QKSFKEARRYCDAQAAASNEADHHTRMPAPASWQLRQGTAYVHICSNETIHGVEYQAL
                     PDLKALGCESPLVVDCSSHILSRPIDWSRVGLAFAGAQKNIGPAGLTLVFVRESLIGH
                     ALPICPSAFDYKTVAEHHSMYNTPPTYAIYVAGLVFQWLKREGGVAEMERRAIARASL
                     LYDYIDASGYYVNRVEKAARSRMNIPFFLPDDALNEAFLAGAKERGLLQLKGHKTVGG
                     MRASLYNAMPHAGVLALVDYMREFQRLHA"
     gene            complement(2054..2641)
                     /locus_tag="VFV25_09900"
     CDS             complement(2054..2641)
                     /locus_tag="VFV25_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HEY1393679.1"
                     /translation="MSKRFWMAALLATGMVMAPAVQAQSKKELVQKLLTLQQPGSEAL
                     GQSIASQTANRVMQAADEGLQRVPADKRAAVGKAMEADIKKFYDDSAAVLRDRAVKIA
                     PAAIGPLLEEKFTEDELKQLIAWAESPVNKKYQQLAPEMENSLVEKLVADTKPTIQPK
                     LATLEQSLRKRLADASGAASAPAASASKPAAKSKN"
     gene            complement(2641..>3804)
                     /locus_tag="VFV25_09905"
     CDS             complement(2641..>3804)
                     /locus_tag="VFV25_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HEY1393680.1"
                     /translation="QIADLLDILAKPERVTFIISEELVALKQEFGQTKVGARRSVIEH
                     NAQELGTEDLITPADMVVTLSHTGYIKSQALSEYRAQRRGGRGKQATATKEDDWIDQL
                     FIANTHDYILCFSNRGRVYWLKVWEVPQGSRNSRGKPIVNMFPLQPDEKINVVLPLTG
                     DFRSFPADHYVFMATAQGTVKKTSLDEFSNPRKAGIIAVKLDEDDYLIGAALTDGHHD
                     VMLFSDGGKAVRFDEDDVRPMGRDTRGVRGMMLEAGQNVIAMLVAEDETQSVLTATEN
                     GYGKRTSIVEYTRHGRGTKGMIAIQQSERNGKVVAATLVRAEDEIMLITDKGVLVRTR
                     VSEIRELGRATQGVTLIALDEGAKLSGLQRIVENDAGEANGAEGGAATDEESV"
BASE COUNT          545 a         1239 c         1325 g          695 t
ORIGIN      
        1 cacgaggctg gtgcaatcgc gccccgaggg gggcggcatg tccagcgtct gcggcaggca
       61 gatcacggcg aagcgggtgc ggttgtaggc gtcgtcctgg atggcgtgcg ccacgatgtg
      121 caggccgtac tgagtggcgg cgcgttcgct ggccagccct gcccacgccg gattggtgga
      181 ggcgaggcgt gcgccctctg cattgctcga cacggcccgg cgctccgctc tcggcagatg
      241 cttggcgagc caggtttggc attgagccag ggcctgcgga tgcgcaagca ctgcctcgat
      301 gccctccagc gagttctcct tgcgcagcag gttgtggcgc accagcaggc tgacctcgcc
      361 gaccacgtgg acgggcgatt gcaggaacat gtccagcgag cgcgtgaccg cgccttcgtt
      421 ggagttttcc acgcccacca cgccgtactg ggcgctgccg ctggtggtgg cgtggaagac
      481 ctcgtcgaag ctggcgcaat agatgaggtc agcggcgcgg ccgaagtatt cgatggcggc
      541 ctgctcgcag aaggtgcctt ccggccccag caccgccacg cgctggggcg actcaagcgc
      601 caggcaggcc gacatgattt cgcgccagat ggccgctacg tgcggcccct tcagcgggcc
      661 ggcattggcg cgctcgatct tctcgatcac ctgggccacc cggtcggggc ggaagaacgg
      721 agtgccctcg cgcctcttga cctcgcccac cagctccgcg atgtgtgccc ggcgattgag
      781 cagcgtcagc agctgctggt cgacttcgtc gatctgggtg cgcaggtcgg cgaggcttcc
      841 ggtgggatcg gagacggaag gcggtggggg ggcggtcatg cgattcacgc gtggagacgc
      901 tggaattctc gcatgtagtc caccagcgcc agcacgccgg cgtggggcat ggcgttgtaa
      961 agcgaggccc gcatgccgcc cacggtcttg tggcccttca gctgcagcag gccgcgctct
     1021 ttcgcgcccg cgaggaaggc ctcgttcaag gcatcgtccg gcaggaagaa ggggatgttc
     1081 atgcgcgagc gggccgcttt ctcgacgcgg ttgacgtagt agcccgaggc gtcgatgtag
     1141 tcgtacagca ggctagcgcg ggcgatcgcg cggcgctcca tctccgccac gccgccctcg
     1201 cgcttgagcc actggaagac caggccggcg acgtagatcg cataggtggg cggcgtgttg
     1261 tacatcgagt gatgctcggc caccgtcttg tagtcgaagg cgctggggca gatgggcagc
     1321 gcatggccga tcagggattc acgcacgaag accagggtca ggccggccgg gccgatgttc
     1381 ttctgcgcgc ccgcgaaggc caggccgacg cgcgaccagt cgatcgggcg cgagaggatg
     1441 tgcgaggagc agtcgaccac cagcggcgac tcgcagccca gggccttgag gtccggcagg
     1501 gcctggtact cgacgccgtg gatggtctcg ttgctgcaga tgtgcacata ggccgtgcct
     1561 tgccgcagct gccagctcgc gggggcgggc atgcgggtgt ggtgatcggc ctcgttgctc
     1621 gccgcggcct gcgcgtcgca atagcggcgc gcctccttga aggatttctg cgaccacgag
     1681 ccggtgacga cgaaatccgc cgcgccgccg cgcgaaaggt tcagcggcac gatggcgttc
     1741 tcggcgatgc cgccgccctg catgaacagg atgcggaagt cctcgggcac ggccagcagc
     1801 tcgcgcagat cggcctgcgc ctgctcgtag atcgagatga attcgcggcc gcggtggctc
     1861 atctccatca cgctcatgcc cgagccttgc cagtcgagca tttcggcagc agcctggctc
     1921 agcacaggct cgggcagcac cgccgggccg gcggaaaagt tgtagggccg ggtcatggct
     1981 ttcgcttcag ctcttcaaca agaaagaaga aaacggccgc ctgcgccgga ccggccgcga
     2041 gacagccagg gactcagttc ttggactttg ccgcgggctt ggatgccgag gcggccggcg
     2101 cggaggccgc gcccgaggca tcggccaggc gcttgcgcag gctctgctcg agggtcgcga
     2161 gcttgggctg tatggtcggc ttggtgtcgg ccaccagctt ctcgaccagg gagttttcca
     2221 tctcgggggc cagctgctga tacttcttgt tgaccggcga ctcggcccag gcgatcagct
     2281 gcttgagctc gtcctcggtg aacttctcct ccagcagggg gccgatcgcg gcaggcgcga
     2341 tcttgacggc gcgatcgcgc aggacggccg cgctgtcgtc gtagaacttc ttgatgtcgg
     2401 cttccatcgc cttgcccacc gccgcccgct tgtcggccgg cacgcgctgc aggccttcgt
     2461 cggcggcctg catcacgcgg ttcgcagtct ggctcgcgat cgactggccc agggcctcgc
     2521 tgcccggctg ctgcagcgtc agcagcttct gcaccagttc cttcttgctt tgggcctgca
     2581 ctgcaggggc catcaccatg ccggtggcga gcagcgccgc catccagaag cgcttgctca
     2641 tcacacgctc tcctcatcgg ttgcagcgcc gccttcggcg ccgtttgctt cgcccgcatc
     2701 gttctcgacg atgcgctgca ggccgctgag cttggcgccc tcgtcgagcg cgatcagcgt
     2761 cacgccttgc gtcgcgcggc ccagctcgcg gatttcagag acgcgggtgc gcaccagcac
     2821 gcccttgtcg gtgatcagca tgatctcgtc ttcggcgcgc accagcgtgg cggcgacgac
     2881 cttgccgttg cgctcgctct gctggatcgc gatcatgccc ttggtgccgc ggccgtggcg
     2941 cgtgtactcg acgatggaag tgcgcttgcc atagccgttc tcggtggcgg tgagcacgct
     3001 ctgggtctcg tcctcggcca ccagcatcgc gatcacgttc tggccggcct ccaacatcat
     3061 gccgcgcacg ccgcgcgtgt cgcggcccat gggccgcacg tcgtcctcgt cgaagcgcac
     3121 cgccttgccc ccgtcgctga acagcatcac gtcgtgatgg ccgtcggtca gcgccgcgcc
     3181 gatcaggtag tcgtcctcgt cgagcttgac cgcgatgatg ccggccttgc gcggattgct
     3241 gaattcgtcg agcgaggtct tcttcaccgt gccctgggcg gtggccatga agacatagtg
     3301 gtcggccggg aagctgcgga agtcgccggt cagcggcagc acgacgttga tcttctcgtc
     3361 gggctgcagc gggaacatgt tgacgatggg cttgccgcgc gaattgcgag agccctgggg
     3421 cacctcccag accttgagcc agtagacgcg gccgcggttg gagaagcaca ggatgtagtc
     3481 gtgcgtgttg gcgatgaaga gctggtcgat ccagtcgtct tccttggtcg ccgtggcctg
     3541 cttgccgcgc ccgccgcggc gctgggcgcg gtactcggac agcgcctgac tcttgatgta
     3601 gccggtgtgg ctgagcgtca ccaccatgtc ggccggcgtg atcaagtcct cggtgccgag
     3661 ctcctgggcg ttgtgctcga tcacgctgcg gcgtgcgccg accttggtct ggccgaactc
     3721 ctgcttcagc gccaccagct cttcgctgat gatgaaggtc acgcgctcgg gtttggcgag
     3781 gatgtccagc aggtcggcga tctg
//
LOCUS       DASUCY010000503         3235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_830859, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000503 DASUCY010000000
VERSION     DASUCY010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830859"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VFV25_09910"
     CDS             complement(<1..303)
                     /locus_tag="VFV25_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008351713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HEY1393681.1"
                     /translation="MNSRAHPHRSRTAAAPHELRIIGGLWKRSKLPVADFPGLRPTPD
                     RVRETLFNWLGQDLSGWRCLDAFAGSGALGFEAASRGAAEVVMLEREPRLAALLRQT"
     gene            468..1496
                     /gene="ftsY"
                     /locus_tag="VFV25_09915"
                     /pseudo
     CDS             468..1496
                     /gene="ftsY"
                     /locus_tag="VFV25_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012839659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
     gene            complement(1622..2350)
                     /locus_tag="VFV25_09920"
     CDS             complement(1622..2350)
                     /locus_tag="VFV25_09920"
                     /inference="COORDINATES: protein motif:HMM:NF022337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HEY1393682.1"
                     /translation="MSSLVVLLAALIVLGLVWMVVRGRKAANQASGKSKPKTMDAVDT
                     VTGWPPDATRLMTTEERRAYILLRQALPNHMILAQVPLARFIKVPTRNSYAEWMRRAG
                     QLCADMLVCDASSKVIAVIDVRRPEIEESDRGHKRHARMDRVLKAAGIQVMVWREDRL
                     PAVSAVREQFLKIQQPAQASAAAGGTKPGMPTKPSDLAPTLDAEVDDDTPQQDPPPST
                     WYDDLESAPMPLSAQSVTSKHWGR"
     gene            complement(2471..>3235)
                     /locus_tag="VFV25_09925"
     CDS             complement(2471..>3235)
                     /locus_tag="VFV25_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEY1393683.1"
                     /translation="LMAEIRELHAALHPIETLFVVDAMQGQDAVNTAKAFKEALPLTG
                     IVLTKLDGDSRGGAALSVRSITGAPIKFAGVSEKIDGLEVFDAERHAGRVLGMGDILA
                     LVEEVHKGVDMEAAQKLAAKVRSGDGFDLNDFLAQISQMKKMGGLSSLMDKLPTQLAG
                     KAGPGDMDRAERDMRRMEGIINSMTPLERRKPELLKASRKRRIAAGAGVQVQEVNRLL
                     NQFEQMQGMMKKMKGGGMMKMMKRMGGLKGLPGMGR"
BASE COUNT          514 a         1097 c         1086 g          538 t
ORIGIN      
        1 cgtctgccgc agcagcgcgg cgagcctggg ttctcgctcc agcatcacca cctcggccgc
       61 gccgcgcgag gcggcctcga agcccaaggc gccgctgccg gcgaaagcat cgaggcagcg
      121 ccagccgctc aagtcctggc ccagccagtt gaacagggtc tcgcgcacgc ggtccggcgt
      181 ggggcgcagg ccggggaaat cggccaccgg cagcttgctg cgcttccaca gcccgccgat
      241 gatgcgcagc tcgtgagggg cggctgcggt gcggctgcga tgcggatggg ctcgggagtt
      301 cacggcgcgc attctaagaa ctcacacatg cttcgacggg atcgaatgct acatatttga
      361 tatcgtattt cgacaaaata attcgggtga tcgttagaaa actcgtcaat ttcgcagcaa
      421 tgacggccag ttcgggccgg cagcaccctc cctagaatcc ccccatcatg ttcagtttct
      481 tcaagaagaa gccggcgccg gccccagagc ccgcagccgc tccagccccg gccgaggcca
      541 gggaccagcc attgaccgag ctcgccgtcg agacgcccga gcagcgccgc tcctggttcg
      601 tccgcctcaa gaccggcctg cgcaagaccg gctccagcat cgcccaggtc ttcaccggca
      661 cccagatcga cgatgcgctc tacgaggagc tcgaggccgc gctgctgatg gccgacgccg
      721 gcgtgggtgc caccgagcat ctgctggccg atctgaagcg gcgcgtgaag gaaagcaaga
      781 caagcgatcc ggccgcagtc aagggcctgc tggccgatgc gcttgccgag ttgctgctgc
      841 ccctggagcg ctcgctggtg ctgggcgaca gcgcgccgac cgtgatgatg gttgccggcg
      901 tcaacggcgc cggcaagact accagcatcg gcaagctcac gcgtcatctg gccgaggccg
      961 atcagaaggt gttgctggcg gcggccgata ccttccgcgc cgcggcgcgc gagcagctcg
     1021 cggtctgggc cgatcgaaac cgtgtggagg tcgtgagcca ggagggcggt gatccggctg
     1081 cggtgtgctt cgatgcggtc taggccggct cggcgcgcgg ctgcgatgtc gtgatcgccg
     1141 actcggccgg gcgcctaccc acccagctgc acctgatgga cgagctgaag aaggtcaagc
     1201 gcaccatcgc caaggcgcgc gagggcgcgc cgcacgaggt gctgctggtg gtggacggca
     1261 acaccggcca gaacgcgctg gcccaggtca agtccttcga cgaggcgctg ggcctcacgg
     1321 gtctcatcgt gaccaagctc gacggcaccg ccaagggcgg cgtgctggcc gcgatcgcgc
     1381 tgtggtcgcg cgcgcaggcg cgggccgtgc cggtctattt catcggcgtg ggcgagaagc
     1441 tggaagacct ggaaaccttc agtgcccgcg agttcgccca ggccctgctg gcctgagcga
     1501 ggacggcgcg cctagcgccg ccgcagggcc agccgggccg ccgcgccggg ccgctcccaa
     1561 gcaaggcccg cccccctctc ggaggggtgg ccgccgtatc cggcgggcgg ggtgctggca
     1621 gctagcggcc ccagtgcttg ctcgtcacgc tctgggcgct gagcggcata ggcgccgact
     1681 cgaggtcgtc ataccaggtc gatggcggcg gatcctgctg cggagtgtcg tcgtcgactt
     1741 cggcgtccag cgtcggcgcg aggtcgctgg gcttggtggg catgccgggc ttggtgccgc
     1801 ctgccgcagc gctggcttgc gcgggctgct ggatcttgag gaactgctcg cgcaccgccg
     1861 aaacagcggg cagacggtct tcgcgccaca ccatcacctg gatgcctgct gccttgagca
     1921 cgcgatccat gcgcgcatgg cgcttgtggc cgcgatcgct ctcctcgatc tcggggcggc
     1981 gtacgtcgat caccgcaatg accttggagc tggcgtcgca caccagcatg tcggcgcaga
     2041 gctggcctgc gcggcgcatc cactcggcat aggaattgcg ggtgggcacc ttgatgaatc
     2101 gggccagcgg cacctgggcc aggatcatgt ggttcggcaa ggcctggcgc agcaggatgt
     2161 aggcgcggcg ctcttcggtc gtcatcaggc gggttgcatc aggcggccag cccgtcacgg
     2221 tatcgaccgc atccatcgtc ttgggcttgg acttgcccga ggcctggttc gcggccttgc
     2281 ggccgcgcac caccatccag accaatccca gcacgatcag cgctgccagc aggacaacaa
     2341 gggaactcat caacgcggca cgaggaagaa acgaaattaa acgaccttaa cggtcaggcc
     2401 tgccaaggtc aatgcggaat tgcgtctttg cgtatccatg gcgggcgcgg cgggcgaagc
     2461 ggctcctcgc tcagcgcccc atgccgggca aacccttgag tccgcccatg cgcttcatca
     2521 tcttcatcat gccgccgccc ttcatcttct tcatcatgcc ctgcatctgc tcgaactgat
     2581 tgagcaggcg gttgacctcc tggacctgca cacccgcgcc cgcggcgatg cgacgcttgc
     2641 gcgaagcttt cagcagctcg ggtttgcgcc tctccaacgg cgtcatcgag ttgatgatgc
     2701 cttccatgcg gcgcatgtcg cgctcggcgc ggtccatgtc gcccgggccg gccttgccgg
     2761 cgagctgggt cggcagcttg tccatcaggc tggacaggcc gcccatcttc ttcatctgcg
     2821 agatctgcgc gaggaagtcg ttgaggtcga agccgtcgcc cgacctgacc ttggcggcca
     2881 gcttctgcgc cgcctccatg tcgacgccct tgtggacttc ctcgaccagg gcgaggatgt
     2941 cgcccatgcc cagcacgcgg ccggcgtgcc gctcggcgtc gaagacctcc aacccgtcga
     3001 tcttctcgga cacgccggcg aacttgatcg gtgcgcccgt gatgctgcgc accgacaagg
     3061 ccgcgccgcc gcgcgaatcg ccgtcgagct tggtcagcac gatgccggtc agcggcaatg
     3121 cctccttgaa ggccttggcc gtattgacgg cgtcctggcc ctgcatcgca tcgacgacga
     3181 agagcgtctc gatcggatgc agcgctgcat gcagctcgcg gatctcggcc atcag
//
LOCUS       DASUCY010000504         2021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1152333, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000504 DASUCY010000000
VERSION     DASUCY010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152333"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..517
                     /locus_tag="VFV25_09930"
     CDS             152..517
                     /locus_tag="VFV25_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEY1393684.1"
                     /translation="MEIQRHEMGPRFSEMTVVRIGDAKLIYIAGQVAENTSLEVAGQT
                     REVLGVIDRLLAQAGATKADIVSVRIYLASVGDYATMNSVWDDWVPPGHTPSRATIGA
                     KLINPEYKIEIEVTAAVQA"
     gene            624..1091
                     /locus_tag="VFV25_09935"
     CDS             624..1091
                     /locus_tag="VFV25_09935"
                     /inference="COORDINATES: protein motif:HMM:NF012342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEY1393685.1"
                     /translation="MRGFTLMELMVVIAVIAILATIALPNLQGRLVRGQIVEAMKLAD
                     IAKGPVAARWSSTKTLPPDNASVGLPSADKIVSNYVSSLSVEGGAIQVTFGNNVNGAV
                     KGKTLSLRPAVVEDAPVVPVAWVCGNASVPDKMTAMGANKTNLPPAFLPLNCR"
     gene            complement(1207..1788)
                     /locus_tag="VFV25_09940"
     CDS             complement(1207..1788)
                     /locus_tag="VFV25_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393686.1"
                     /translation="MAGIRAAALLLAGMVLAAANAHAQGVYRCSNGAFSDRPCGGPQT
                     SLTVIGPVQTHVDPAPSYIPPVGKAPEHLQYLSPACASLNDAMRTAPARGLRGQALGD
                     LQAEYRAKCDEEDQAARARVFQDKLAQRNERRSQQVAQQAEHSRQLATREQCSEMLRI
                     LHGKRQQQGTMTSGEKADFQHFEDNYTARCKAP"
BASE COUNT          333 a          693 c          679 g          316 t
ORIGIN      
        1 ggccgagcag gccgccttgc aggcgcacct aggcgtcgtg caggcccgcg ccaatcgcct
       61 gtcggactcc gtggcgctgt tccaggctct ggggggcggc tggtggaatc ggcaagaatc
      121 gctgacgcaa gcaccgcaat aacgacgcac catggaaatc caacgccacg agatgggccc
      181 ccgcttcagc gagatgacgg tcgtgcgcat cggcgacgcg aagctgatct acatcgccgg
      241 ccaggttgcc gaaaacacct cgctcgaagt ggccggccag acccgcgagg tgctgggcgt
      301 gatcgaccgc ctgctggccc aggccggcgc caccaaggcc gacatcgtct cggtacgcat
      361 ctacctcgcc tcggtgggcg actacgcgac gatgaactcg gtctgggacg attgggtgcc
      421 gcccggccac accccgtctc gggcgaccat cggcgccaag ctgatcaatc ccgagtacaa
      481 gatcgagatc gaagtcactg ccgcggtgca ggcctgagga aatggaccac ccccgaagcg
      541 ccgtaggcgc tccccctcaa gggggcgacg ccagcgaacc ggcaaagccg gatccgcgac
      601 gtccgcttgg caaaacccgg acggtgcgcg gtttcacgct gatggagctg atggtcgtga
      661 tcgccgtgat cgcgatcctg gcgacgatcg cgctgcccaa tcttcagggg cggctggtgc
      721 gcggccagat cgtcgaggcg atgaagctgg ccgacatcgc gaagggcccg gtcgccgcgc
      781 gctggagcag cacgaagacg ctgccgcccg acaacgcgag cgtcggcctg ccgagcgccg
      841 acaagatcgt cagcaactac gtcagctcgc tcagcgtaga aggcggcgcc attcaggtca
      901 ccttcggcaa caacgtcaac ggcgccgtca aaggcaagac gctgagcctg cgccccgccg
      961 tcgtcgagga cgccccggta gtgccggtgg cctgggtctg cggcaatgcc tcggtgccgg
     1021 acaagatgac cgcgatgggc gcgaacaaga ccaacctccc gccggccttc ctgccgctca
     1081 actgccgcta gatcatgaca cccggtcgcg gactaccgcg accaccctcc gagagggtgc
     1141 cggcttgctg ggagcggccc ggcgcagcgc cggcctggcc tatcgctagc acgcactgaa
     1201 caggcatcag ggcgccttgc agcgggctgt gtagttgtcc tcgaagtgct ggaagtcggc
     1261 cttctcgccg ctcgtcatcg tgccctgctg ctggcgcttg ccgtgcagga tgcgcagcat
     1321 ctcgctgcac tgctcgcgcg tcgcgagctg gcgcgaatgc tccgcctgtt gcgccacctg
     1381 ctgggaccgg cgctcattgc gctgggcgag cttgtcctgg aacacccgcg cacgcgcggc
     1441 ctggtcctcc tcgtcgcatt tggcgcggta ctcggcctgc aggtccccta gcgcctggcc
     1501 gcgcaggccg cgggcgggcg cggtgcgcat ggcgtcgttg agcgaagcgc aggccgggct
     1561 caggtactgc agatgctccg gcgccttgcc gacgggcggg atatagctcg gcgccgggtc
     1621 gacatgagtt tggaccgggc cgatcaccgt gagcgaggtc tgcggcccgc cgcaggggcg
     1681 atcggaaaaa gcaccattgc tgcagcggta gacgccctgg gcatgcgcgt ttgcggccgc
     1741 caggaccatc ccggccagga gcaaagctgc tgctcgaatg cctgccatcg tctacctcat
     1801 tcttcgattc ttgttggcag gcagtgtatc gggcagcatc cggcgccgct tccctctttc
     1861 aggggttggg ccgcgaccgg aacaccgcgg cagccagacc gccatgatca gaaatgaatg
     1921 ccctgcatca tctgctgcag cgcgatggcc tcggccgaca gctggggctt gccgcccttg
     1981 tcgccccgcg ccgcctcgct gtcggggaac atgcggaagc t
//
LOCUS       DASUCY010000505         2333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_397030, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000505 DASUCY010000000
VERSION     DASUCY010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397030"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VFV25_09945"
     CDS             complement(<1..408)
                     /locus_tag="VFV25_09945"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEY1393687.1"
                     /translation="MSSVYDFEAQSIAGESLHLSKYRGKVMLIVNTASACGFTPQFAG
                     LETLWKKHGQEGLVVLGFPCNQFGSQDPGSNEEIAGFCQLNYGVSFPMMSKIEVNGSG
                     AHPLYQWLTKEAKGILGTQAIKWNFTKFLVGKDG"
     gene            586..1527
                     /locus_tag="VFV25_09950"
     CDS             586..1527
                     /locus_tag="VFV25_09950"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY1393688.1"
                     /translation="MIKRHRVALLGFSAFERRTLESYFRLAGDGTPHYEQYALLPDVV
                     PEPEACDFVLADANHGEVLAVVTAMGREADTVYIGTHLAPPAPAGALASLLRPIDPLQ
                     VLREFDAAAARRRRVAPPALAHMLRPEPAPHHPRKPSARTRIAAAQQSLRDFQSTDGH
                     SNSVLTTGGDSLFDHVLVVDDSEIARRFMQVKLQRLGFTVHTVASGAAALELAATRQF
                     AFVFLDVNMEGMDGFEVCKQIRQMPWPLDGRLQRGPVLVMVTARSAPVDRIRGTLAGC
                     DAYLTKPLDEAELIGTLSRHDPCFERVFDDTLPPPLP"
     gene            1644..>2333
                     /locus_tag="VFV25_09955"
     CDS             1644..>2333
                     /locus_tag="VFV25_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY1393689.1"
                     /translation="MDFSIASILLLDGATNGAIYALLAISLVLLFAVTRVIYIPQGEY
                     VAFSALTMGMFQLGKTPGTVWFLLIMSAAVALLDLAQGLRRGDAFLAVLWRVLRATAF
                     PVAVAMLSIWIAPRQPSLWLQALLTLALVTPFGALIYRLAYQSLADATVLVLLIVSVG
                     VHFALTGAGLLFFGAEGFRNPDFWDRRFELGPLTLSGQALIIFLASAALIVMLWLFFE
                     RTLYGKALRATA"
BASE COUNT          330 a          829 c          744 g          430 t
ORIGIN      
        1 gccgtccttg ccgaccagga atttcgtgaa gttccacttg atggcctggg taccgaggat
       61 gcccttggcc tccttggtga gccactggta gagcggatgg gcgccgctgc cgttgacctc
      121 gatcttgctc atcatcggga agctcacgcc gtagttgagc tggcagaagc cggcaatttc
      181 ctcgttgctg cctgggtcct gcgagccgaa ctggttgcag gggaagccca gcaccaccag
      241 gccctcctgc ccatgcttct tccacagcgt ctccaggccc gcgaactggg gcgtgaagcc
      301 gcaggcgctg gcggtgttga cgatcagcat gaccttgccg cggtacttcg acaggtgcag
      361 gctctcgcca gcgatggact gggcctcgaa gtcgtagacg ctgctcatga ctgctcgctc
      421 ctgctgcaag aacctagggt ctgttgaccc tcgatggtag ccccccgatg caggggatgc
      481 tcaaacaggc catgcacgtg aatttccccc tgcccttcag ccgcccttca tgagaacatt
      541 acgctgtcgt tgcaacggct cctcgcccgc atccttggcc gctccatgat caaacgccac
      601 cgcgtggcct tgctgggatt cagcgccttc gagcgcagga cgctcgagtc ctacttccgc
      661 ctcgcgggcg acggcacccc gcactacgag caatacgcgc tgctgcccga cgtggtcccc
      721 gagcccgagg cctgcgattt cgtgctggcc gatgccaacc atggcgaggt actggcggtg
      781 gtgaccgcca tgggccgcga ggccgatacg gtctatatcg gcactcacct cgcgccccca
      841 gcgcccgccg gggccctggc gagcctgctc cgccccatcg accccttgca ggtactgcgc
      901 gaattcgacg ccgcagccgc ccgccgccgg cgcgttgcgc cgccggcgtt ggcgcacatg
      961 ctgcggcccg aaccagcgcc tcatcacccg cgcaagcctt ccgcccgcac gcgcatcgca
     1021 gcggcacagc agagcctgcg cgacttccag tccaccgacg gtcacagcaa ttcggtgctg
     1081 accacgggcg gcgacagcct gttcgaccat gtgctggtgg tggacgacag cgagatcgcg
     1141 cggcgcttca tgcaggtcaa gctgcagcgc ctgggcttca cggtgcacac ggtggccagc
     1201 ggcgccgcgg cgctggagct ggcggcgacg cgtcagttcg ccttcgtctt cctcgacgtc
     1261 aacatggaag gcatggacgg tttcgaggtc tgcaagcaga tccgccagat gccctggcca
     1321 ctggacggcc gcctgcagcg cgggccggtg ctggtgatgg tgacggcgcg cagcgcgccg
     1381 gtggaccgca tacgcggcac gctggccggc tgcgacgcct atctcaccaa gcccttggac
     1441 gaggccgaat tgatcggcac cctgtcgagg cacgacccct gcttcgagcg cgtgttcgac
     1501 gacacgctgc cgccgcccct gccctgatcc tcgccttggg cgaatccgga tagccagccg
     1561 ccgcgcctgc ggcgatcatg ccggggccgt ggcacattac gcggcgccag aagaatgggc
     1621 gcatcgcccg cagaacgatc tccatggact tctccatcgc cagcatcctg ctgctcgacg
     1681 gcgcgaccaa cggcgcgatc tacgcgctgc tggcgatctc gctcgtgctg ctgttcgccg
     1741 tcacccgcgt catctacatc ccccagggcg aatacgtcgc cttcagcgcc ctcacgatgg
     1801 gcatgttcca gctcggcaag acgcccggca cggtgtggtt cctgttgatc atgtcggccg
     1861 cggtggcgct gctcgatctg gcgcagggcc tgcgacgggg cgatgccttc ttggccgtgc
     1921 tgtggcgggt gctgcgcgcg acagccttcc cggtcgccgt ggctatgctc agcatctgga
     1981 tcgcgccgcg tcagccgtcg ttgtggctgc aagcgctgct gacgctggcc ctggtcacgc
     2041 ccttcggcgc gctgatctat cgcctggcct atcagtcgct cgccgacgcg acggtgctgg
     2101 tgctgctgat cgtctcggtg ggtgtgcact tcgcgctgac cggcgccggc ctgctgttct
     2161 tcggcgccga gggctttcgc aacccggact tctgggaccg gcgcttcgag ctcggcccgc
     2221 tcacgctgtc gggccaggcg ctgatcatct tcctcgcctc ggcggcgctg atcgtgatgc
     2281 tgtggctctt cttcgagcgc acgctgtacg gcaaggccct gcgcgccacc gcc
//
LOCUS       DASUCY010000506         3342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1473420, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000506 DASUCY010000000
VERSION     DASUCY010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473420"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(333..1340)
                     /locus_tag="VFV25_09960"
     CDS             complement(333..1340)
                     /locus_tag="VFV25_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEY1393690.1"
                     /translation="MDNVDLNILRQVAAWLAEGHKVVLGTITRTWGSAPRPVGSVMGV
                     RDDGRVAGSVSGGCIEDDLIAKVRAGSLDLAAPTVLVYGIGAEEATRFGLPCGGTLEL
                     VLEPLGTQSQVPALLEKLTRGELVSRVLDLKTGAVQLTPPSDGDVVRLSETQLVTTHG
                     PRWRLLVIGAGQMTHYLASMAQALDYEVLICDPREEYANGWDVPGARLTRGMPDDTVT
                     EFRPDGHCAVVALTHDPKLDDLALMEALKSNAFYVGAIGSRVNQAKRRQRLAEHFGLT
                     PEQLARLHGPIGLKNGARTPPEIAVSILAEMTAVRYGYHVPEPLPPASGAGEAEPGCA
                     I"
     gene            1617..2087
                     /locus_tag="VFV25_09965"
     CDS             1617..2087
                     /locus_tag="VFV25_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY1393691.1"
                     /translation="MAIKISLKVNGKAVSREVDPQTLLVSFLREDLRLTGTHVGCDTA
                     QCGACTVHLNGRAVKSCNMLAVQAQGAEITTIEGLSANGSLHPMQAAFKECHGLQCGY
                     CTPGMVMSAVDLVQHHPHSTEQQIREALEGNFCRCTGYQNIVKAVQQGAQAMKA"
     gene            2101..>3342
                     /locus_tag="VFV25_09970"
     CDS             2101..>3342
                     /locus_tag="VFV25_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018314795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEY1393692.1"
                     /translation="MNAPQGFIGQSIKRKEDARFLTGRGQYTDDISLPGQSYGYFLRS
                     PYAHARIKRIATQAAGAAEGVLGVFTGEHFRAVGGLPCGWLINSVDGTPMKEPKHPIL
                     ADGKACYVGDAVALVVAETLEQAKAAAALVEVDYDELPAVADTGGAKGAKGVVHDEAP
                     DNVCYTWAVGDKAAVDAAMSSAAHVTKLQFKNNRLIPNAIEPRAANASYNVADDSYTL
                     YVANQNPHVERLLMCAFVLGIPEHKVRVVAPDVGGGFGSKIFLYAEETALTWASKQVG
                     RPIKWTAERSEAFLSDAHGRDHVTTAELATDKDGHFLAMRVHTTANLGAYLSTFASCV
                     PTILYATLLAGQYATPKIYCQVTGVFTNTAPVDAYRGAGRPEATYVVERLVETAAHEL
                     NIDPAEIRRRNFIKSFPYATPV"
BASE COUNT          574 a         1138 c         1113 g          517 t
ORIGIN      
        1 gatgcgcgcg cgtggagcgc gaccgaaatg gggaggtgcg tgggggccgc gcagaacgcg
       61 ctgcccgagg aaagggagct agaagctcct gagaaaaacg ctccgtcccg ctgctatcac
      121 agcgagacgg cggacatgga agacggcccg aggtctcgct gttcagttcg ccttgcgcga
      181 actcctacta gggccagaat ccatggattg ctccgttgtt gcagtgcatc gaatttttct
      241 cttcggacct gggcatgtca accgtgggcc tttgccttgg ggggtggtcc ggtcgatctg
      301 cggcggatgc gcaacgccgc cggcaggttt gctcagatcg cgcagccggg ctcggcctcg
      361 ccggcgccgc tggccggcgg tagcggttcc ggcacgtggt agccgtagcg cacggccgtc
      421 atctcggcca ggatggagac cgcgatctcg ggcggggtgc gcgcgccgtt cttcaggccg
      481 atcgggccgt gcagtcgcgc gagctgctcc ggcgtgaggc cgaaatgctc ggccaggcgc
      541 tggcgccgct tggcctgatt gacgcgggag ccgatcgcgc cgacatagaa ggcgttcgac
      601 ttcagcgcct ccatcagcgc caggtcgtcc agcttggggt catgggtcag cgcgacgacg
      661 gcgcagtggc cgtcgggcct gaactcggtc acggtgtcgt ccggcatgcc gcgcgtaagc
      721 cgtgcgccgg gcacgtccca gccgttggcg tattcctcgc gcgggtcgca gatcagcact
      781 tcgtagtcca gcgcctgggc catagacgcg aggtagtgcg tcatctggcc cgcgccgatg
      841 accagcagcc gccagcgcgg gccgtgggtc gtcacgagct gggtctcgct taggcgcacc
      901 acgtcgccat cgctgggggg ggtgagctgc acggcgccgg tcttcaggtc cagcacgcgg
      961 ctcaccagct cgccgcgcgt cagcttctcc agcagcgccg ggacctgtga ctgcgtcccc
     1021 agcggctcca gcaccagctc cagcgtgccc ccgcagggca ggccgaagcg cgtcgcctcc
     1081 tccgcgccta tgccatagac cagcaccgtc ggcgcggcca ggtcgaggct gccggcgcgc
     1141 accttggcga tcaggtcgtc ctcgatgcag ccgccggaca ccgagccggc cacgcggccg
     1201 tcgtcgcgca cccccatgac cgagccgacc gggcgcggcg ccgaacccca ggtgcgtgtg
     1261 atcgtgccca gcacgacctt gtgaccttcg gccaaccagg cggccacctg gcgcaggatg
     1321 ttcagatcga cgttgtccat ggtgatgttc taaccccgga ttggcgaagg cggccctggc
     1381 tggccaccga gaaagctagc ttagcccggg atccgcgtca tccaggaccg aacccgcagc
     1441 cggcggcggc gctgtaccaa aaagggacag cgggccaaat gcccattcga cgcgaagcct
     1501 gctcgccctg cattgctagg gtttttgcgc gatagttgcg cttctcctgt cgtcctagga
     1561 tgactcgaaa gccaaccgcg acggccgcgt tcgcccacca gcttcgagga gacatcatgg
     1621 ccatcaagat ttccctgaag gtcaatggca aggctgtgag ccgggaggtc gacccccaga
     1681 ccttgctcgt cagcttcctg cgcgaagact tgagacttac cggcacccat gtgggctgcg
     1741 acaccgcaca atgcggagcc tgcaccgtgc acctcaacgg gcgcgcggtc aagtcctgca
     1801 acatgctggc ggtgcaggcc caaggcgccg agatcaccac catcgaaggg ctgtctgcga
     1861 acggcagcct gcatcccatg caggccgcct tcaaggagtg ccatggcctg caatgcggct
     1921 actgcacgcc gggcatggtg atgagcgccg tcgatctcgt tcagcatcac ccgcactcca
     1981 ccgagcagca gatccgcgag gccctggagg gcaacttctg ccgctgcacc ggctaccaga
     2041 acatcgtgaa ggcggtccag cagggcgccc aggcgatgaa ggcatgacga ggaggccgcc
     2101 atgaacgcac cccaaggctt catcgggcaa agcatcaagc gcaaggaaga cgcccgcttc
     2161 ctcaccggcc gcggccagta caccgacgac atctcgctgc caggccagag ctacggctat
     2221 ttcctgcggt cgccctatgc gcatgccagg atcaagcgca tagccacgca ggcggccggc
     2281 gcggccgagg gcgtgctggg cgtgttcacc ggcgagcatt tccgcgcggt gggcggcctg
     2341 ccctgcggct ggctgatcaa cagcgtcgac ggcacgccga tgaaggagcc caagcatccc
     2401 atcctggccg acggcaaggc gtgctacgtg ggcgatgctg tggccctggt cgtggccgag
     2461 accctggagc aggccaaggc cgccgccgcc ttggtcgagg tggactacga cgagctgccc
     2521 gccgtggccg acaccggcgg cgccaagggg gccaagggcg tggtgcacga cgaggcgccc
     2581 gacaacgtct gctacacctg ggccgtcggc gacaaggcgg ctgtcgatgc ggcgatgagc
     2641 agcgcggcgc atgtgaccaa gctgcagttc aagaacaacc ggctgatccc caacgcgatc
     2701 gagccgcgtg ccgccaacgc gagctacaac gttgccgacg acagctacac gctctatgtg
     2761 gccaaccaga acccgcatgt cgagcgcctc ttgatgtgcg ctttcgtgct cggcatcccc
     2821 gagcacaagg tgcgcgtggt ggcgccggac gtgggcggcg gcttcggctc caagatcttt
     2881 ttgtacgccg aggagacggc cctcacctgg gccagcaagc aggtcggccg gccgatcaag
     2941 tggacggcag agcgaagcga ggccttccta agcgatgccc atggccgcga ccacgtcacc
     3001 accgccgagc tcgccaccga caaggacggg catttcctgg cgatgcgcgt gcacaccacg
     3061 gccaacctcg gcgcgtatct ctcgaccttc gcctcctgcg tgccgaccat cctgtatgcg
     3121 accctgctgg cagggcagta cgcaacgccc aagatctatt gccaggtcac cggtgtgttc
     3181 accaatacag cgccggtcga tgcctatcgc ggcgcgggcc ggcccgaggc gacctacgtg
     3241 gtcgagcgtc tggtcgagac ggcggcacac gaactcaaca tcgacccggc cgagatccgg
     3301 cggcgcaact tcatcaagag cttcccgtat gcgacgccgg tg
//
LOCUS       DASUCY010000507         3701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1232443, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000507 DASUCY010000000
VERSION     DASUCY010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232443"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..928)
                     /gene="ubiA"
                     /locus_tag="VFV25_09975"
     CDS             complement(47..928)
                     /gene="ubiA"
                     /locus_tag="VFV25_09975"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEY1393693.1"
                     /translation="MTTTAAPSPRSRLALYLDLIRWDRPAGWLLLLWPTLATLWLASG
                     GFPGWHLLAVFTLGTVLMRSAGCCINDVADRDFDRYVKRTAQRPVTSGAVSVREALLL
                     GAALALVAFTLVLTTNALTIALSFAALAVTLAYPYAKRYVSMPQAVLGVAFSFGIPMA
                     FAAAQGSVPALVWGLLIGNLFWVLAYDTEYAMVDRDDDLHIGIKTSAITLGRYDVIGV
                     MGFYAVYLLSWAVLGWSQGLRWLFLLGIAAAAAQALWHYTLIRNRSREGCFKAFRANH
                     WLGFAVFAGTALDLALR"
     gene            complement(1065..1562)
                     /locus_tag="VFV25_09980"
     CDS             complement(1065..1562)
                     /locus_tag="VFV25_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HEY1393694.1"
                     /translation="MAKNAKKVPPISIGISDKDRAAIAKGLNELLADTYTLYLTTHNF
                     HWNVTGPMFNTLHLMFMGQYTELWNAVDPIAERIRALGYPAAGSYGQFGKLSSLQDAP
                     ASPPEAMGMVRILVSGHEAVARTARRVFAVADQANDQPTADLLTQRLDIHEKSAWMLR
                     ALLEA"
     gene            complement(1664..>3701)
                     /gene="katG"
                     /locus_tag="VFV25_09985"
     CDS             complement(1664..>3701)
                     /gene="katG"
                     /locus_tag="VFV25_09985"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012327929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HEY1393695.1"
                     /translation="NYAAEFKTLDYAAVKRDLNDVMTSSQDWWPADYGHYGPFFIRMA
                     WHSAGTYRVADGRGGGGRGQQRFSPLNSWPDNGNLDKARRLLWPIKQKYGRKLSWADL
                     MILAGNVALESMGFKTLGFGGGRPDTWEPDQDVYWGSETAWLDDKRYSGDRELETPLG
                     AVQMGLIYVNPEGPNGNPDPVAAARDIRETFARMAMNDEETVALIAGGHTFGKAHGAA
                     PASHVGANPEGADLEAQGFGWKSSYVSGKGGDAITSGLEVTWTTTPTKWSNDFFRHLF
                     GYEWELTKSPAGAHQWVAKGAADIIPDAQDPSKKRRPTMLTTDLSLRFDPAYEKISRR
                     FMEHPEQFADAFARAWFKLTHRDMGPRARYLGPEVPAEDFIWQDPVPAADHKQIDAQD
                     IAALKAKVLASGLTVSQLVSTAWASASTFRGSDKRGGANGARIRLAPQEDWEVNQPEQ
                     LAQVLKTLEGIRAEFNGKAFGGRQVSLADLIVLAGCAGVEQAAKNAGASVTVPFAPGR
                     TDASAEQTDTESFAVLEPVADGFRNFQKGKFPVPAEALLIDKAQLLTLTAPEMTVLVG
                     GLRVLGANAGGAKHGVFTSKPQALTNDFFVNLLDMGTEWKPVSDAKDVYEGRDRKTGE
                     LKWTGTRADLVFGSNSVLRALAEVYGSADAKGKFVDDFVAAWNKVMNLDRFDLA"
BASE COUNT          580 a         1194 c         1304 g          623 t
ORIGIN      
        1 gaccggcgga gccggatccg tggcgtgtgc ttggcaatac ctagcgtcag cgtaaagcta
       61 agtcgagcgc ggtacctgca aacactgcga agcctagcca gtggttcgcg cggaaggcct
      121 tgaagcagcc ttcgcggctg cggttgcgga tcagcgtgta gtgccacagc gcttgggccg
      181 ctgcggctgc gatgccgagc agaaatagcc agcgcagccc ctggctccag cccagcacgg
      241 cccagctcag cagatagacc gcgtagaagc ccatcacgcc gatcacgtca tagcggccca
      301 gcgtgatggc cgaggtcttg atgccgatgt gaagatcgtc gtcgcggtcg accatcgcgt
      361 actcggtgtc gtaggccagc acccagaaca gattgccgat cagcagcccc cagacgaggg
      421 cagggacgct gccttgcgcg gcggcgaagg ccatggggat gccgaagctg aaagccacgc
      481 cgaggacggc ctgcggcatc gagacatagc gcttggcgta ggggtaggcg agcgtcactg
      541 cgagggcggc gaaggacagc gcgatcgtca gcgcattggt cgtcagcacc agcgtgaagg
      601 ccacgagcgc cagcgcggcg ccgagcagca aggcctcgcg caccgacacg gcgccgctcg
      661 tcacgggccg ttgcgccgtg cgcttcacgt agcggtcgaa gtcgcggtcg gccacgtcgt
      721 tgatgcagca gccggcgctg cgcatcagga ccgtgcccag cgtgaagacg gccagcagat
      781 gccagccggg gaagccgccc gaggccagcc acaaggttgc cagcgtgggc cacagcagga
      841 gcagccagcc ggccgggcga tcccagcgga tcaggtcgag atacagcgcc aggcgcgaac
      901 gcggcgatgg ggcggcagtc gtggtcacag cgcggaatta tccgcgcgtg accttcgctg
      961 cgcttcagct ttcaaacggg ctgcgcagca tccacgcggt cttggctacg acccacatcg
     1021 ctgcgcgata tgagtgtccg ccgaagccgc atggagtggg gcgctcaggc ttccagcaag
     1081 gcgcgcagca tccacgcgct cttctcgtga atatccaggc gctgcgtcag caggtcggcg
     1141 gtgggctggt cgttggcctg gtcggcgacg gcgaagacgc ggcgcgcggt gcgggccacg
     1201 gcctcatggc cgctgaccag gatgcgcacc atgcccatcg cttcgggcgg cgaggccggt
     1261 gcatcctgca gcgagctgag cttgccgaac tggccatagg agccggcggc cgggtagccc
     1321 agggcgcgga tgcgctcggc gatgggatcc acggcgttcc acaactcggt gtactggccc
     1381 atgaacatca ggtgcagggt gttgaacatc ggccccgtga cattccagtg gaagttgtgc
     1441 gtcgtcaggt agagcgtgta ggtgtcggcc agcagctcgt tcaagccctt ggcgatggca
     1501 gctctgtcct tgtcgctgat gccgatgctg atcggcggga ctttctttgc gttcttggcc
     1561 atgggtgcaa ccctccggtg cggctaggag tagaagactt tagcgcagtg aggcctctct
     1621 ggcaggccat gcctcgcgca tcagcgaggc atggcccttg gcatcacgcg aggtcgaagc
     1681 ggtcgaggtt catcaccttg ttccaggcgg cgacgaagtc gtcgacgaac ttgcctttcg
     1741 catccgcgct gccatagacc tcggccaggg cgcgcagcac ggagttggag ccgaagacga
     1801 gatcggcgcg ggtgccggtc cacttgagct cgcccgtctt gcggtcgcgc ccttcgtaga
     1861 cgtccttggc atccgacacc ggcttccact cggtgcccat gtcgagcagg ttcacgaaga
     1921 agtcgttggt cagcgcctgt ggcttgctgg tgaagacgcc atgctttgcg ccgccggcgt
     1981 tggcgcccag cacgcgcagg ccgccgacca gcaccgtcat ctccggcgcg gtcagggtca
     2041 gcaattgcgc cttgtcgatc agcagcgcct cggccggcac cgggaacttg cccttctgga
     2101 agttgcggaa gccgtcggcg accggctcca gcacggcgaa ggactcggta tcggtctgtt
     2161 cggcagaggc atcggtgcgg ccaggcgcga acggcaccgt cacgctggcg ccggcgttct
     2221 tcgcggcctg ctcgacgccc gcgcaacccg ccagcacgat caggtcggcg agcgagactt
     2281 gtctgccgcc gaaggccttg ccgttgaact cggcccggat gccttccagc gtcttcagca
     2341 cctgggccag ctgctcgggt tggttgactt cccagtcctc ctgaggcgcc aggcggatgc
     2401 gcgcgccgtt ggcgccgccg cgcttgtcgg agccgcggaa ggtggaggcc gaggcccagg
     2461 cggtggacac cagctgcgag accgtgaggc ccgaggccag caccttggcc ttgagggcgg
     2521 cgatgtcctg cgcatcaatc tgcttgtgat cggcggcggg cacggggtcc tgccaaatga
     2581 agtcttcggc aggcacctcg gggccgaggt agcgcgcacg tgggcccatg tcgcggtggg
     2641 tcagcttaaa ccaggcgcgc gcgaaggcgt cggcgaactg ctcggggtgc tccatgaagc
     2701 gccgcgagat cttctcgtag gccgggtcga agcgcagcga gaggtccgtg gtcagcatgg
     2761 tgggccggcg cttcttggac gggtcttggg catcgggaat gatgtcggca gcgcccttgg
     2821 cgacccactg gtgcgcaccg gccgggctct tggtcagctc ccattcgtag ccgaacaggt
     2881 gcctgaagaa gtcgttgctc cacttcgtcg gcgtggtggt ccaggtgacc tccaggccgc
     2941 tggtgatcgc gtcgccgccc ttgccgctga catagctgct cttccagccg aagccctgcg
     3001 cctcgagatc ggcgccttca ggattcgccc ctacgtgcga ggccggggcg gcgccgtggg
     3061 ccttgccgaa ggtgtggccg ccggcgatca gcgcgaccgt ttcctcgtcg ttcatcgcca
     3121 tgcgggcgaa ggtctcgcgg atgtcgcggg ccgccgcgac cgggtcggga ttgccgttcg
     3181 ggccctcggg gttgacgtag atcagcccca tctgcacggc gccgagcggc gtctcgagtt
     3241 cgcggtcgcc cgagtagcgc ttgtcgtcca gccaggcggt ctcggagccc cagtacacgt
     3301 cctggtcggg ctcccaggtg tccggccggc cgccgccgaa gcccagagtc ttgaagccca
     3361 tcgattccag cgcgacgttg ccggcgagga tcatcaggtc ggcccatgac aacttgcggc
     3421 cgtacttctg cttgatcggc cacagcagcc ggcgcgcctt gtcgaggttg ccgttgtcgg
     3481 gccagctgtt gagcggcgag aagcgctgct ggccccgccc gccgccgccg cggccgtctg
     3541 ccacgcgata ggtgccggcg ctgtgccagg ccatgcggat gaagaacggg ccatagtggc
     3601 cgtagtctgc gggccaccag tcctgcgacg acgtcatgac gtcgttgaga tcgcgcttca
     3661 ccgcggcgta gtcgagcgtc ttgaactcag cggcgtagtt g
//
LOCUS       DASUCY010000508         2416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_220595, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000508 DASUCY010000000
VERSION     DASUCY010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220595"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VFV25_09990"
     CDS             <1..200
                     /locus_tag="VFV25_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393696.1"
                     /translation="ERYNTGSAYADIGTGLTPNPRPTERVVTLGANFKLNSSVVIKAD
                     VQRFKEASDNDRFDLGLGWSF"
     gene            203..745
                     /locus_tag="VFV25_09995"
     CDS             203..745
                     /locus_tag="VFV25_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HEY1393697.1"
                     /translation="MRIVMLAGTAAALAPASAFAVDYLSAEQAARLMFPEADQFESRT
                     VDLDAAELQHFEATTGVRARSAHWPLRLAKKNGAVLGYVVTDEVIGKFELIGYAVGLT
                     ADGAVKQIEILSYRESHGGEIRLPAWRRQFVGKTAASPIKVGEDIANISGATLSCSHV
                     TDGVRRIVALVDLARRSGKL"
     gene            742..1482
                     /locus_tag="VFV25_10000"
     CDS             742..1482
                     /locus_tag="VFV25_10000"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEY1393698.1"
                     /translation="MSTPGPSGWQRRARPLLGTLVEVGAPADAPPQAIAAAFAAVERV
                     QALMSRFDPASDVARFNALARGEAIAIKASTAEVMHAARELELASGGLFDVSAGCPQA
                     WRLQGLELAKQSSEVKLDLGGIAKGYAVDLAVQAMQAAGCEGGWVNAGGDARAFGPAM
                     LPILLRDEMAGGVRPFVSLSEGAIATSHYGPGSRSQVWRGGPSLHAHVSVAAPQALWA
                     DALTKIVALSGDTAHPLLNRHQARAWLH"
     gene            1470..1925
                     /locus_tag="VFV25_10005"
     CDS             1470..1925
                     /locus_tag="VFV25_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HEY1393699.1"
                     /translation="MAALNPLDRWQRRGLYWSCGLLVASGVVWLVLHYGWGAGAGELP
                     HPLESWLMRLHGACAFAALFLAGVVAAQHVPRGWRITHAHPRMRLRWAFHRRSGLTLC
                     TLGGLLAASGYALYYFAPETVRPALGWAHTGLGLALAGLLTAHARPRAD"
     gene            2120..2374
                     /locus_tag="VFV25_10010"
     CDS             2120..2374
                     /locus_tag="VFV25_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HEY1393700.1"
                     /translation="MPSLALSDLPNGALGTVSRVVPASALADAQMLRRLEELGFLPGE
                     PVRLLRRGPGGREPLAVQIGDTLFALRKVEAQCVQVEVAS"
BASE COUNT          354 a          826 c          850 g          386 t
ORIGIN      
        1 acgaacgcta caacacgggt agcgcctatg cggatatcgg tacgggcttg acgccgaatc
       61 cccgtccgac cgagcgcgtc gtcacactgg gcgccaactt caagctcaac agcagcgtcg
      121 taatcaaggc cgatgtgcag cgcttcaagg aggcgagcga caacgaccgc ttcgacctgg
      181 gtcttgggtg gagcttctga ccatgcgcat cgtcatgctg gccggaacgg ccgccgcgct
      241 ggcgccggcc tccgccttcg cggtggatta cctgagtgcc gagcaggcag caaggctgat
      301 gtttcccgag gccgatcagt tcgagtcacg caccgtcgat ctcgatgccg ccgagctgca
      361 gcacttcgag gcgaccacag gcgtgcgcgc tcgctcggcg cattggccgc tgcgtctcgc
      421 caagaagaac ggcgcggtgc tggggtatgt cgtgacggac gaggtgatcg gcaagttcga
      481 gctgatcggc tatgcggtag gcctcacggc ggacggtgcg gtcaagcaga tcgagatcct
      541 cagctatcgc gaaagccatg gcggcgagat ccgcctgccg gcctggcgca ggcagttcgt
      601 cggcaagacg gcggcctcgc cgatcaaggt cggcgaggac atcgccaaca tcagcggcgc
      661 gactttgagt tgcagccatg tcaccgatgg cgtgcggcgc atcgtcgcgc tcgtcgactt
      721 ggcgcgccgt tcggggaagc tgtgagcacg ccggggccct ccggctggca gcggcgcgcg
      781 aggccattgc tgggcacgct ggtggaggtc ggcgcgccgg ccgatgcgcc tccgcaggcg
      841 atcgccgcag cctttgctgc ggtcgagagg gtgcaggcgc tgatgtcgcg tttcgatccg
      901 gccagcgatg ttgcgcgctt caacgctctg gcccgcggcg aagccatcgc catcaaggcc
      961 tcgacggccg aggtgatgca tgccgcgcgc gaactggagc tggctagcgg tggcctgttc
     1021 gatgtctcgg ccggctgccc gcaggcctgg cggctgcagg ggctggaact cgccaagcaa
     1081 tcaagcgaag tgaagctcga cctgggcggc attgccaagg gctatgccgt cgacctggcg
     1141 gtacaggcga tgcaggcggc cggctgcgaa ggaggctggg tcaatgcggg cggcgatgcg
     1201 cgcgccttcg gcccggccat gctgcccatc ctgctgcgcg acgagatggc gggcggcgtg
     1261 cgccccttcg tgagcctgag cgagggcgcc atcgccacca gccattacgg cccgggcagc
     1321 cgcagccagg tctggcgcgg cggcccatcg ctgcacgcgc atgtcagcgt cgcagcgccg
     1381 caagcgctgt gggccgacgc gctgaccaag atcgttgcgc tcagcggcga tactgcgcac
     1441 cccctgctca accggcatca agcgcgcgca tggctgcact gaatcctctc gatcgctggc
     1501 agcgccgcgg cctgtactgg agctgcggcc tgctggtcgc cagcggcgtc gtctggctgg
     1561 tgttgcatta cggctggggc gccggcgcgg gcgagctgcc gcatccgctg gaaagctggc
     1621 tgatgcgcct gcacggcgcc tgcgccttcg ccgccctctt cctggccggc gtcgtcgcgg
     1681 cccagcatgt gccgcgcggc tggcgcatca cccatgcgca cccgcgcatg cgcctgcgct
     1741 gggccttcca tcgccgcagc ggcctgacgc tgtgcacgct cggcggcctg ttggcggcga
     1801 gcggctacgc gctctactac ttcgcgcccg agacggtgcg gccggctctc ggctgggctc
     1861 acacagggct ggggctggcg ttggccggct tgctgacggc ccatgcccgg ccacgcgcag
     1921 actgatcgcc cccaaggccg gggctgcgcc cggccccacc ccccgcgggg gtcaaggaaa
     1981 cttggggcgg cggcccggcg tttccttgag agacgacagc agggccattt gactcaaatc
     2041 aatctaaatg cgaccgaatc gcatttagaa ttgcatcctc ggcgctcggc tttgcgccgc
     2101 cattggatcg cccgccctca tgcccagcct cgccctcagt gacctgccca acggcgcctt
     2161 aggcacggtc agccgcgtcg tgcccgcttc ggcgctggcc gacgcgcaga tgctgcgccg
     2221 gctcgaagag ctgggcttcc tgccgggcga gccggtgcgc ctgctgcgcc gcggcccggg
     2281 cggccgcgag ccactggcgg tgcagatcgg cgacaccctg ttcgcgctgc gcaaggtcga
     2341 ggcgcaatgc gtgcaggttg aagtcgcatc atgagccaag cccaagcaat ggacctgagc
     2401 gcttgccgca tcgcgc
//
LOCUS       DASUCY010000509         4227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_862859, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000509 DASUCY010000000
VERSION     DASUCY010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862859"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VFV25_10015"
     CDS             <1..189
                     /locus_tag="VFV25_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY1393701.1"
                     /translation="ELNDQPLPVSNGAPLRCRIERQLGYKHAKYVRAIELVEGFAQIR
                     GGKGGYWEDQGYEWYAGI"
     gene            complement(218..1456)
                     /gene="glcF"
                     /locus_tag="VFV25_10020"
     CDS             complement(218..1456)
                     /gene="glcF"
                     /locus_tag="VFV25_10020"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HEY1393702.1"
                     /translation="MQTHLAPEYKSTPDGDAADAILRKCVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGQAPTRKTQLHLDRCLTCRNCESTCPSGVQYGQLVEIGRRVV
                     DAKVERPAAESAQRWLLKEGLTSPAFAPAMKLGQMLRPLLPAALQNKVPAGAGARAQL
                     WPQRRHPRKMLLLLGCVQPAMLPNINSATARVLDAAGIQTLVADEAGCCGAIRAHLGD
                     HEGGLADMRRNIDAWWPLVQKSEVEAIVMNASGCGVTVKEYGQHLAHNPAYAERAQRI
                     SELTRDLSELLPELLPKLKPLLRQPKTRRLAFHPPCTLQHGQQLRGGVESGLRELGFE
                     VELAASESHLCCGSAGTYSVLQPELAYQLRDRKLEQLTPLKAQAIVSANIGCIQHLQS
                     GTTTPVRHWIEVVDEALAER"
     gene            complement(1469..2560)
                     /gene="glcE"
                     /locus_tag="VFV25_10025"
     CDS             complement(1469..2560)
                     /gene="glcE"
                     /locus_tag="VFV25_10025"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017333241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HEY1393703.1"
                     /translation="MNQDDHLQPFIEHVRAASAAGTPLRIIGSGSKDFYGGPLQGELL
                     ETAQLSGISSYEPTELVVTARAGTPLAELEAALAERGQSLAFEPPRYGGKGTVGGMVA
                     AGLSGPARATAGALRDYVLGATLLNGKAEVLSFGGQVMKNVAGYDVSRLLAGAMGTLG
                     LILEVSLKVLPQAPASATLRFELDQASALRRLNTWAGQPLPINASAWWENMLVLRLQG
                     AQAAVKAASALLGGELIETETAAKFWNGLRDQEDEFFVSAAKAVANGASLWRLSLPPT
                     APPLHVSGELLIEWGGAQRWLCSSASAEAIREAAARLGGHATLFRALDKSAGAFAPLQ
                     PPLDRIHRALKQAFDPAGIFNRGRLYAEF"
     gene            complement(2561..4051)
                     /locus_tag="VFV25_10030"
     CDS             complement(2561..4051)
                     /locus_tag="VFV25_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY1393704.1"
                     /translation="MNTSATQPEERAVRQAEVVAALQRLLPAYALLWQREDTTPYECD
                     GLTCYRELPLVVALPETEEQVATVLKACHDLSVPVVARGAGTGLSGGALPHAMGVTLS
                     LAKFNRILRIDPLARTAAVQSGVRNLAISEAVAAHGLYYAPDPSSQIACTIGGNVAEN
                     SGGVHCLKYGLTLHNVLKLRGYTVAGEAITLGSDALDAPGLDLLSVFIGSEGMLGVVT
                     EVTVKLLPKPTLARCIMASFDDIRKAGDAVANIIAEGIIPAGLEMMDKPMTAAVEDFV
                     HAGYDLSAEAILLCESDGTPEEVNEEIGRMLDVLVGSGATRVEVSKNEAQRLKFWSGR
                     KNAFPASGRISPDYMCMDSTIPRKRLADILIAIAQMETKYGLRCANVFHAGDGNLHPL
                     ILFDANDPDQLHRAELFGADILETSVEFGGTVTGEHGVGVEKLNSMCVQFSPAERDQM
                     LALKQAFDPAGLLNPGKVIPTLARCAEYGKMHVKRGLLPFPELPRF"
     gene            4044..>4227
                     /locus_tag="VFV25_10035"
     CDS             4044..>4227
                     /locus_tag="VFV25_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393705.1"
                     /translation="MFIEWRSESGVLPGSTLLRGQLQQSARDVLAFDGGAKAGGVTSN
                     SEDRQHAGVAVEMMARD"
BASE COUNT          625 a         1425 c         1470 g          707 t
ORIGIN      
        1 gagttgaatg accagccctt gccggtcagc aacggcgcgc cgctgcgctg ccgcatcgag
       61 cgccagctcg gctacaagca cgccaagtat gtaagggcga tcgaactggt cgagggcttt
      121 gcgcagatcc gcggcggcaa gggcggctat tgggaggatc agggctacga gtggtatgcg
      181 gggatttgag tcacccccgc ggcgccggcc ggctgcatca gcgctccgcc agcgcctcgt
      241 cgaccacttc gatccaatgc cgcaccggcg tcgtggtgcc gctttgcagg tgctggatgc
      301 agccgatgtt ggccgagacg atggcctggg ccttcagcgg cgtgagttgc tcgagcttgc
      361 ggtcgcgcag ctgataggcc agctcgggct gcaggaccga ataggtgccg gccgagccgc
      421 agcacagatg gctttcgctg gccgccagtt ccacctcgaa acccagctcg cgcagacctg
      481 actcgacgcc gccgcgcagc tgctgcccat gctgcagcgt gcaaggcggg tggaaggcga
      541 ggcgcctggt cttcggctgg cgcagcagtg gtttgagctt gggcagcagt tcgggcagca
      601 gctcgctcaa gtccctggtc aattcgctga tgcgctgcgc ccgttcggca taagccgggt
      661 tatgggccag atgctggccg tattccttga cggtcacgcc acagcccgag gcgttcatca
      721 cgatggcctc gacctcgctt ttctgtacca gcggccacca ggcatcgatg ttgcggcgca
      781 tgtcggccag gccgccctcg tggtcgccca gatgggcgcg gatcgcgcca cagcagccgg
      841 cctcatcggc caccagggtc tggatgccgg ccgcgtcgag tacgcgcgcc gttgcgctgt
      901 tgatgttggg cagcatggcc ggctgcacgc agcccagcag cagcagcatc ttgcgcgggt
      961 ggcggcgctg cggccagagc tgggcgcgcg caccggcccc ggccggcacc ttgttctgca
     1021 gcgcggcagg cagcagcggg cgcagcatct gccccagctt catcgccggc gcgaaggccg
     1081 gcgaggtgag gccttccttc agcagccagc gctgggcgct ttcggccgcc ggtcgctcga
     1141 ccttggcgtc gacgacgcgc cggccgatct cgaccagttg gccgtattgc acgcccgagg
     1201 ggcaggtgct ctcgcagttg cggcaggtca ggcagcggtc cagatgcagc tgcgtcttgc
     1261 gcgtcggcgc ctggccctcg agcacctgct tgatcagata gatgcgcccg cgcgggccgt
     1321 ccagctcgtc gcccagcagc tgataggtcg ggcaggtcgc ggtgcagaag ccgcaatgca
     1381 cgcacttgcg caggatggcg tcggcggcgt cgccgtcggg cgtgctcttg tactcgggag
     1441 cgaggtgggt ctgcatctgc gtcgtggctc agaactcggc gtacagccgc ccgcggttga
     1501 agatgccagc cggatcgaag gcctgcttca aggcgcggtg gatgcgatcc agcggcggct
     1561 gcagcggcgc gaaggcgccg gccgatttgt ccagcgcgcg gaacagtgtt gcatggccgc
     1621 ccagccgcgc cgcggcctcg cgtatcgcct cggcgctggc gctgctgcac agccagcgct
     1681 gggcgccgcc ccattcgatc agcagctcgc ccgagacatg cagcggcggc gccgtgggcg
     1741 gcagcgacag ccgccacagg ctggcgccgt tggccacggc cttggcggcg ctcacgaaga
     1801 actcgtcttc ctggtcgcgc aggccgttcc agaacttggc cgccgtttcg gtttcgatca
     1861 actcaccgcc gagcaaagcc gaggcggcct tcaccgcggc ctgcgcgcct tgcagacgca
     1921 gcaccagcat gttttcccac cacgcgctgg cattgatcgg cagcggctgg ccggcccagg
     1981 tattgagccg gcgcagcgcg cttgcctggt ccagctcgaa gcgcagcgtc gcgctggcgg
     2041 gcgcctgcgg cagcaccttc agcgagacct cgaggatcag gcccagcgtg cccatagcgc
     2101 cggccagcag gcgcgagacg tcgtagccgg ccacgttctt catcacctgg ccgccgaagc
     2161 tcagcacctc ggccttgccg ttgagcaggg tggcgcccag cacatagtcg cgcagggcgc
     2221 ccgcggtggc gcgcgccggc cccgaaagcc cggccgccac catgccgccc accgtgccct
     2281 tgccaccgta gcgtggcggc tcgaaagcca ggctttggcc gcgctcggcc agcgctgcct
     2341 ccagctcggc aagcggcgtg cccgcgcgtg ccgtgacgac cagctcggtc ggctcgtagc
     2401 tgctgatgcc gctgagctgg gccgtctcca gcagctcgcc ttgcagcggc ccgccgtaga
     2461 agtccttgct gccgctgccg atgatgcgca gcggcgtgcc ggccgcactg gcagcgcgca
     2521 cgtgctcgat gaagggctgc agatggtcgt cttggttcat tcaaaagcgc ggcaactcgg
     2581 ggaagggaag caagccgcgc ttgacatgca tcttcccgta ctcggcgcag cgcgcgaggg
     2641 tggggatgac cttgcctgga ttgagcaggc cggccggatc gaaggcctgc ttcagggcta
     2701 gcatctgatc gcgttcggcg gggctgaact gcacgcacat cgagttgagc ttctcgacgc
     2761 ccacgccatg ctcgccggtc acggtgccgc cgaactccac gctggtttcc aggatgtcgg
     2821 cgccgaagag ctcggcgcgg tgcaactggt cggggtcgtt ggcgtcgaac aggatcagcg
     2881 ggtgcaggtt gccgtcgccg gcatgaaaga cattggcgca gcgcagtccg tatttggtct
     2941 ccatctgcgc gatcgcgatc aggatgtcgg ccaggcgctt gcgcgggatg gtcgagtcca
     3001 tgcacatgta gtcggggctg atgcggcccg aggcgggaaa ggcgttcttg cgcccgctcc
     3061 agaacttcag gcgctgcgct tcgttcttgc tcacctcgac gcgcgtggcg ccggagccga
     3121 ccagcacgtc gagcatgcgg ccgatctcct cgttgacttc ctcgggcgtg ccgtcgcttt
     3181 cgcacagcag gatggcctcg gcgctcaagt cgtagcccgc gtgcacgaag tcctcgaccg
     3241 ccgccgtcat cggcttgtcc atcatctcca ggccggccgg gatgatgccc tcggcgatga
     3301 tgttggcgac cgcatcgccc gccttgcgga tgtcgtcgaa gctggccatg atgcagcgcg
     3361 ccagcgtggg tttgggcagc agcttgaccg tcacctcggt cacgacgccc agcatgccct
     3421 cgctgccgat gaagacagac agcagatcga ggcccggcgc atcgagcgcg tcgctgccca
     3481 gcgtgatggc ctcgcccgcc accgtgtagc cgcgcagctt gagcacgttg tgcaaggtga
     3541 ggccgtattt caggcaatgc acgccgcccg agttctcggc cacgttcccg cctatggtgc
     3601 aggcaatctg gctgctcggg tcgggcgcgt agtagaggcc atgcgcggcc accgcctcgc
     3661 tgatcgcgag attgcgcacg ccgctttgca cggcggccgt gcgcgccagc gggtcgatcc
     3721 tgaggatgcg gttgaacttg gcgagcgaca gcgtcacgcc catcgcatgc ggcagggcgc
     3781 cgccggacag gccggtgccg gcgccgcgtg cgaccaccgg cacgctcaag tcgtggcagg
     3841 ccttgagcac cgtggccacc tgctcctcgg tctcgggcag cgcgaccacc agcggcagtt
     3901 ctcgatagca ggtaaggcca tcgcattcat aaggcgtcgt gtcctcgcgc tgccatagca
     3961 gggcataggc cggcaacagg cgctgcagcg ctgccaccac ctcggcctgg cggacggcgc
     4021 gctcttcggg ctgagtggcg ctggtgttca tcgagtggcg ctcggaaagt ggtgttctgc
     4081 ctgggtcgac tttacttcgc gggcagctcc agcaaagcgc acgcgacgtc ctcgcctttg
     4141 atggcggcgc aaaggcgggc ggcgtcacat caaacagtga agaccgtcag cacgccggtg
     4201 tagccgtaga gatgatggcg cgcgatc
//
LOCUS       DASUCY010000510         2551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1473504, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000510 DASUCY010000000
VERSION     DASUCY010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473504"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="VFV25_10040"
     CDS             <1..850
                     /locus_tag="VFV25_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY1393706.1"
                     /translation="VPPDNKNSGQAIAALIAEKASPVADVTYLGGIAADPAKNAGVLA
                     AYKPKNWDRIPADLKDPAGHWFTIHSGTLGLLVNTAALGKAAVPSSWSDLLKPEYKGL
                     VGYLDPTSAAVGQLGVMAVNLALGGSYENLDAGIRYFQALSKNQPIVPKQTAYARVLS
                     GEIPILVDYDFNAYRAQYTDKAPVRFVIPKEGTLVFPYVMGLVSNAPHAENGKKVLDF
                     VLSDESQTMWGNAFLRPVFTDRLSKEAKSKFLPDSDYARAKAVDLKKLAAAQPRIVER
                     YKAEVQ"
     gene            855..1673
                     /locus_tag="VFV25_10045"
     CDS             855..1673
                     /locus_tag="VFV25_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY1393707.1"
                     /translation="MRPGERHAAWLLLPAALFFLAFFLLPMGRLLLLGGSGGQGWSAY
                     AAIVTDPGYRRALISTLVLAAATTAATLAIAGVAGVFLQRHRFAGRSALVSMLTLPLA
                     FPGVVVGFMVIMLAGRQGLIGRLSEVLVGDRLVFAYSIAGLFMGYLYFSIPRALLTLM
                     AAAEKLDPRLEEAARSLGASTWRVMVDVILPGLKPALISTGAICFATSVGAFGTAFTL
                     ATRIDVLPMLIYTEFTLAANIAMAAALSFALGAVTWAVLAVARSAAGTTVAAGA"
     gene            1680..2468
                     /locus_tag="VFV25_10050"
     CDS             1680..2468
                     /locus_tag="VFV25_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY1393708.1"
                     /translation="MTSRRWLFGMQLGFTLLVCAFLVLPVGLSILAGLTSNYFIGPSS
                     GLTLRWVAEVWASYRDTLLLSVEIALACLAMCLLLGVPAAYALARLRGRGARLFEELL
                     VMPVAVPGLATALGLIVAYGGFGAFRTSAAFILVGHVLFTLPFMVRSVLAVLSAIDLQ
                     TLEESAASLGAGPSRRFFDIVLPNCRNGILAGSLMVVTLSMGEFNMTWLLHTPLTKTL
                     PIGLADSYASMQLEVASAYTLIFFIMIIPLLVAMQLFADKEHAR"
BASE COUNT          361 a          881 c          829 g          480 t
ORIGIN      
        1 ggtgccgccc gacaacaaga actcgggcca ggccatcgcc gcgctcatcg ccgagaaggc
       61 cagtccggtg gcagacgtca cctatctcgg cggcatcgcc gcggaccccg ccaagaacgc
      121 cggcgtgctc gccgcctaca agccgaagaa ctgggaccgg attcccgctg atctcaagga
      181 ccctgccggc cactggttca ccatccattc cggcacgctg ggcctgctcg tgaacaccgc
      241 ggcgctgggc aaggcggcgg taccaagcag ctggtccgac ctgctcaagc cggagtacaa
      301 ggggctggtc ggctatctcg accccacctc ggcggcggtc ggccagctcg gcgtgatggc
      361 cgtcaatctg gcgctgggcg gcagctatga aaacctggat gccggcatcc gctacttcca
      421 ggccttgtcc aagaaccagc ccatcgtgcc caagcagacg gcctatgcgc gcgtgctctc
      481 cggcgaaatc cccatcctgg tcgactacga tttcaacgcc taccgcgccc agtacaccga
      541 caaggcgccg gtgcgcttcg tgattccgaa ggagggcacg ctggtgttcc cctacgtgat
      601 ggggctggtg agcaacgcgc cgcacgcgga aaacggcaag aaggtgctcg acttcgtgct
      661 gtccgacgag agccagacca tgtggggcaa tgcctttctg aggccggtct tcacggatcg
      721 cctgtccaag gaagccaagt ccaagttcct gcccgatagc gattacgccc gtgccaaggc
      781 cgtcgacctg aagaagctgg ccgccgcgca gcccaggatc gtcgagcgct acaaggcgga
      841 agtgcagtag gccgatgcgc cccggcgaac gccatgccgc gtggctgctg ctgccggcag
      901 ccctcttctt cctggccttt ttcctgctgc cgatgggtcg cctgctgctg ctgggcggca
      961 gcggggggca ggggtggtcg gcctatgcgg cgatcgtcac cgacccgggc tatcggcgcg
     1021 cgctgatttc gacgctggtg ctggccgcag ccaccacggc agccacgctc gccattgccg
     1081 gggtcgcagg cgtgttcctg cagcgccatc gcttcgccgg gcgctccgca ctcgtctcga
     1141 tgttgaccct gccgctggcc ttccccggcg tggtggtcgg cttcatggtc atcatgctgg
     1201 cggggcggca aggcctgatc gggcgcctca gcgaagtcct cgtcggcgac cggctcgtgt
     1261 tcgcctattc gatcgccggc ctgttcatgg gctatctgta tttctcgatt ccgcgcgcgc
     1321 tgctcaccct gatggccgct gccgagaagc tcgacccgcg cctggaagag gcggcccggt
     1381 ctctcggcgc ttcgacgtgg cgggtcatgg tcgacgtgat cctgcccggc ctgaagccgg
     1441 ccctcatttc gaccggggcg atctgcttcg cgacctcggt cggggcgttc ggcaccgcct
     1501 tcacgctcgc tacgcgcatc gacgtgctgc cgatgctgat ctacaccgaa ttcacgctgg
     1561 cggccaacat cgcgatggcg gcggccctca gctttgcgtt gggagcggtc acctgggccg
     1621 tgctggcggt ggcgcgaagc gcagccggaa ccaccgtggc agcgggggcc tgattcagca
     1681 tgacctctcg tcgctggctc ttcggcatgc agctcggatt cacgctgctg gtctgcgcct
     1741 tcctcgtgct gccggttggc ttgtcgatcc tggccgggct gacgtccaac tacttcatcg
     1801 gtccgagcag cggcctcacg ctgcgctggg tcgccgaggt ctgggccagc tatcgcgaca
     1861 cgctgctcct ctcggtggaa atcgcactgg cctgtctcgc catgtgcttg ctgctcggcg
     1921 tgccggcggc ctatgcgctg gcccgcctgc gcggccgcgg cgcgcgcctg ttcgaggagc
     1981 tgctggtgat gcccgtcgcg gtgcccgggc tggccaccgc gctcggcctg atcgtcgcct
     2041 atggcggctt cggcgcattc cgcacttcgg cggccttcat cctcgtgggc catgtgctgt
     2101 tcaccttgcc cttcatggtg cgctcggtgc tggccgtgtt gtcggcgatc gacctgcaga
     2161 ccctggaaga gagcgcagct tcgctcggcg ccggaccgtc ccggcgcttc ttcgacatcg
     2221 tgctgcccaa ctgccgcaat ggcatcctcg ccggctcgct gatggtggtg acgctgtcca
     2281 tgggcgagtt caacatgacg tggctgctgc acaccccgct gaccaagacg ctgccgatcg
     2341 ggctcgccga cagctatgcc tcgatgcagc tggaagtcgc gtccgcctat accctgatct
     2401 ttttcatcat gatcattccg ctgctggtcg ccatgcagct ctttgccgac aaggagcatg
     2461 cgcgatgagc gagggacacg gcgccgccgt gcgcatcgag aattgcggca agacttttac
     2521 cgacgggacc cgcgcgctgg agcccgcgac g
//
LOCUS       DASUCY010000511         3087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1554007, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000511 DASUCY010000000
VERSION     DASUCY010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1554007"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..1562
                     /locus_tag="VFV25_10055"
     CDS             60..1562
                     /locus_tag="VFV25_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393709.1"
                     /translation="MDTVIKTSAVPLPRSLGLSWPLLVAMLAIVAVLGLDPAARVLGD
                     PDSYWHVASGRWIVEHASIPSRDPFSYSLQGAPWTAHEWLSEIAMYGIYRAGGWAGLV
                     VMAACCFALVLARLTRFLMDRMEPAHALLFVGFTAGMLNSHLLARPHVMAWLLLAAWT
                     SKLIDAVESRRAPPGWLLALMVLWANMHLSFTLGLLLAGALALEALLAGPVAERRRMA
                     WVWGRFLLLAIAASVLTPHGWESYAYTWDVMQQKVAMANIGEWLSPNFQQPHPLEVWV
                     LLMLGIALTGRAQMGWLRVLLILGLVHLALKHQRNVDVLGLVTPFLLATPLARSWQAT
                     SKGGDVEALDRWFRSFAAPARPLAVAVCTVAIALFVVGAAQWRDDKPAASITPKAAME
                     AAMAAGARGRVLNDYGFGGYLIQQGIPVFIDGRSDMYGDRFLEEWLAAMRLKDRKSFL
                     DFLEAHRFGWSLLPPGTPAIAVLDDLPGWRRIYADETAVVHVREAEGDRR"
     gene            1577..2602
                     /locus_tag="VFV25_10060"
     CDS             1577..2602
                     /locus_tag="VFV25_10060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY1393710.1"
                     /translation="MDRANFHQAAAEAALVGIRECAASPLLFDGRPALAIVVPCFNEE
                     EVLPETARCLAALMESLIAGGEISAASGIYFIDDGSTDRTWARIEALAAQTTLFHGVK
                     LAHNSGHQNAVLVGLFQVSGDALVTIDADLQDDPEAIREMLAAFRGGAEVVYGVRRRR
                     DSDSFFKRVTAEGYYRLLGALGVEVVFNHADYRLMSRCAVDALQRYGECNLFLRGIVP
                     KLGFSHATVYYDRGERLAGESKYPLGKMLALAVQGVTSFSAAPLRAITMLGFVVSLLS
                     FLGALWVVWVRLMGTHVVPGWASIVVPVFFLGGIQLLSLGVIGEYIAKIYMETKRRPR
                     YLVDRNI"
     gene            complement(2613..2948)
                     /locus_tag="VFV25_10065"
     CDS             complement(2613..2948)
                     /locus_tag="VFV25_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006750400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393711.1"
                     /translation="MPWRLYACLLTGSLCAACGQLLFKLGATGRGTLASYLNLHILTG
                     LGLYALGTALWIAALSQAKLTTVYPFTALTFVLVYGMGIWVLGEAVSNQALAGVAMVL
                     FGLFLIAAA"
BASE COUNT          487 a          981 c         1042 g          577 t
ORIGIN      
        1 ctcgactagc cagcgcacgc tcgctagagt gcttcgaact gaagtcgaat cgtctgaaca
       61 tggacaccgt tatcaaaacc tcggctgttc ctttgcctcg ctcgctgggc ctgtcgtggc
      121 cgctgctggt ggccatgctc gcgatcgtgg cggtgctggg cctggatccg gcagcgcgcg
      181 tgctgggtga tcccgattcg tattggcacg tggcttcggg ccgctggatc gtggagcacg
      241 cgagcatccc ctcgcgcgac cccttctctt actcgctgca aggtgcacct tggacggctc
      301 acgagtggct gtccgagatt gccatgtatg gcatctaccg ggccggcggc tgggccggct
      361 tggtggtgat ggcggcctgc tgcttcgcac tggtgctcgc tcgcctgacg cgttttctga
      421 tggaccgcat ggagccggcg catgcgctgc tgtttgtcgg cttcactgcg ggaatgctga
      481 acagccacct gctggcgcgc ccgcacgtca tggcctggct gctgctggcc gcgtggacgt
      541 cgaagctgat tgacgcggtg gagtcgcggc gcgcgccccc cggctggctg ctggcactga
      601 tggtgctctg ggccaacatg cacttgagct tcacgctggg cctcttgctg gctggcgcgc
      661 tggcactcga ggccttgttg gccggaccgg ttgccgagcg ccggcgcatg gcatgggtct
      721 gggggcgctt ccttctactg gcgattgccg ccagcgtgct gactccgcat ggctgggagt
      781 cctacgccta tacctgggac gtgatgcagc agaaggtcgc catggcgaac atcggcgaat
      841 ggctctcgcc caacttccag cagccgcatc cgctcgaggt ctgggtgctg ctgatgctgg
      901 gtattgcctt gacaggccgc gcgcagatgg gctggctgcg ggtgctgctg atactgggac
      961 tagttcatct cgcgctgaag catcagcgca acgtggacgt cctggggctg gtcacgccct
     1021 tcctcctcgc gactcccctg gcgcgcagct ggcaggccac gtccaaaggc ggagatgtag
     1081 aggcgctgga tcgctggttc cggtcattcg cggcacccgc gcgcccgctt gccgttgccg
     1141 tctgcacggt ggccatcgct ctcttcgtcg tcggtgcagc tcagtggcgc gacgacaagc
     1201 cggccgcttc catcacgccc aaggctgcca tggaggcggc aatggcggct ggtgcccgcg
     1261 gccgggtgct caatgactac ggcttcggcg gctacctgat tcagcagggt attccggtct
     1321 tcatcgacgg gcgctccgac atgtatggcg atcgcttcct cgaagaatgg ctggcagcca
     1381 tgcggctcaa ggaccgcaag agcttcctcg atttcctcga agcgcatcgc tttgggtggt
     1441 ccttgctccc gcccggaacc cccgccatcg ccgtgctgga tgatttgccg ggatggcgac
     1501 gcatctacgc cgacgaaaca gcggtcgtgc acgtgcgcga agccgaaggc gacaggcgct
     1561 gaagaaggga ggcgagatgg accgtgcgaa cttccaccaa gccgcagccg aggcagcgct
     1621 ggtcggcatc cgcgaatgcg ccgcgagtcc gctcctcttc gacggcaggc ctgctctggc
     1681 catcgtcgtt ccatgcttca atgaagagga agtgctgccc gagacggcgc gctgcctggc
     1741 cgcgctgatg gagagtttga ttgcgggcgg cgaaatctcg gctgccagtg gcatctactt
     1801 catcgacgac ggctccaccg accggacctg ggcgaggatc gaagcgctgg cggcacagac
     1861 gacgctgttc catggagtca agctggccca caacagcggc caccagaatg cagtgctggt
     1921 cgggctgttc caggtgtcgg gcgatgccct ggtcacgatc gatgccgatc tgcaggacga
     1981 ccccgaggcg atacgcgaga tgctggcggc cttccgcggc ggagccgagg tcgtctacgg
     2041 cgtgcgccga cgccgcgaca gcgacagctt cttcaagcgc gtcacggcgg agggctacta
     2101 ccggctgctc ggcgcattgg gtgttgaagt ggtcttcaat cacgccgact accggctgat
     2161 gtcgcgctgt gccgtcgacg cgctgcaacg ctacggcgag tgcaatctct tcctgcgcgg
     2221 gatcgtccca aagctgggct tcagccacgc caccgtgtac tacgaccgcg gcgagcggct
     2281 ggccggcgaa tccaagtacc cgctgggcaa gatgctggcg ctggcggtgc agggcgtcac
     2341 ctcgttctcc gccgcaccgc tgcgcgccat cacgatgctc ggcttcgtcg tgtcgctgct
     2401 cagcttcctg ggggcgttat gggtcgtctg ggttcgcctc atgggtactc acgtggtacc
     2461 gggctgggcc tccatcgtcg tgccggtgtt cttcctcggc ggcatccagc tcctgagcct
     2521 gggcgtaatc ggcgaataca tcgccaagat ctacatggag accaagcggc gcccgcgcta
     2581 tcttgttgat cgcaacatct gaggaagacg gttcaagccg cagcgatcag gaacaggccg
     2641 aacagcacca tggcaacccc cgcaagggcc tgattcgaga cggcttcgcc gagcacccat
     2701 attcccatcc catagaccag tacgaaggtc agggcggtga aggggtagac ggtggtgagc
     2761 ttggcctgcg aaagcgctgc gatccagaga gccgtgccca gtgcgtaaag tcccaggccc
     2821 gtcaggatgt gaaggttgag gtaagacgcc agcgtgccgc gcccggtcgc gccgagcttg
     2881 aagagcagct gcccgcaggc cgcgcacagc gagcccgtca gcaggcaggc gtagaggcgc
     2941 cagggcatct ttttagcggg aggaagccac cgccctgcga tgcttccaga tggcgagcct
     3001 ggtcaccgcc ggggcgggca ggcagcgcag cagccacttg cgcagccgat aggcgggcgg
     3061 cgacaagtcg gcgatacgaa cgtcgtc
//
LOCUS       DASUCY010000512         3280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_317073, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000512 DASUCY010000000
VERSION     DASUCY010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317073"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="VFV25_10070"
     CDS             <1..738
                     /locus_tag="VFV25_10070"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEY1393712.1"
                     /translation="IDLHRAGGIPQVMKVLLKAGLLHGDCITITGKTVTENLADVPDA
                     PRADQDVIRAIDRPIYVEGHLAILRGNLATEGCVAKITGLKNPVITGSARVFDDEQSA
                     LAAIMAGGIKPGDVMVLRYLGPKGGPGMPEMLAPTGALIGQGLGESVGLVTDGRFSGG
                     TWGMVVGHVAPEAYEGGTIALVQEGDSVTIDAHKLLLQLNVGDAELAQRRAAWKAPAP
                     RYTRGVLAKFAKNASSASSGAVLDKFD"
     gene            complement(740..985)
                     /locus_tag="VFV25_10075"
     CDS             complement(740..985)
                     /locus_tag="VFV25_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04438 family Trp-rich protein"
                     /protein_id="HEY1393713.1"
                     /translation="MWFVVIGVLLLVMKVADFGPVGGWSWLLVLAPFACAVAWWAWAD
                     ASGYTKRREMDKVDAVREERRKRNLASLGLPTRRKRR"
     gene            1094..1402
                     /locus_tag="VFV25_10080"
     CDS             1094..1402
                     /locus_tag="VFV25_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY1393714.1"
                     /translation="MKRLLTLLAAALLAGPALADATADLAQKKNCMACHAIDKKLVGP
                     AYKDVAAKYAGQKDAVAKLTQKVLKGGSGAWGPVPMPANTQVNEAEAKQLVQWVLSQK
                     "
     gene            1532..2026
                     /locus_tag="VFV25_10085"
     CDS             1532..2026
                     /locus_tag="VFV25_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEY1393715.1"
                     /translation="MTTTKPVTPTSSAAFEPEFVQRLKTIFEESISFNRLLGLRIDEV
                     ASERVTAHLEMRPELIGHFAHHRLHGGVISATLDAMGGLAVMAAIGARHMDEALEARL
                     TRFGKLGTIDLRIDYLRPATGPRFVAVAEVLRLGSRVATTRMEFLDAQGRLLSTGAAA
                     YIVS"
     gene            complement(2066..3088)
                     /locus_tag="VFV25_10090"
     CDS             complement(2066..3088)
                     /locus_tag="VFV25_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HEY1393716.1"
                     /translation="MHAAAQAIPEYAGLDETSIRRDILTQYRPAILRGLVRDWPAVRE
                     AAVSAAALSSYLKALDSGREVDAIMTPPEERGRIFYNADLSGFNFLRNRLPLSKVIEQ
                     VLRYSLFDRPPSVAAQSALIPDCLPGFVEENRLGLLDAAVQPRIWLGNAITTPAHFDE
                     ADNIACVVSGRRRFTLFSPEQIANLYVGPLDYTPTNTPISLVDFEAPDFERHPRFRKA
                     LAAAHVGELGPGDAIFIPTLWWHHVVSLEKFNVLVNYWWNGSIGTAGRTDSGLAGLLH
                     SLLNLRQLSSPQRQAWSAIFEHYLFGDREAAAAHIPPHLRGVLGEMSDEQVARLRALL
                     RSGLEG"
BASE COUNT          598 a         1104 c         1100 g          478 t
ORIGIN      
        1 atcgacctgc accgcgccgg cggcatcccg caggtgatga aggtgctgct caaggccggc
       61 ctgctgcacg gcgactgcat caccatcacc ggcaagacgg tgaccgagaa cctggccgac
      121 gtgcccgatg ccccgcgtgc cgaccaggac gtgatccgcg ctatcgaccg acccatctat
      181 gtcgaaggcc acctcgccat cttgcgcggc aacctggcca ccgagggctg cgtggccaag
      241 atcacgggcc tcaagaaccc tgtcatcacc ggctcggcgc gcgtgttcga cgacgagcag
      301 tcggcgctcg ccgccatcat ggccggcggg atcaagccgg gcgatgtgat ggtcttgcgc
      361 tacctgggcc ccaagggcgg cccgggcatg ccggagatgc tggcccccac cggggcgctg
      421 atcggccagg gcctgggcga aagcgtgggg ctggtcaccg acggccgctt ctcgggcggc
      481 acctggggca tggtggtggg gcacgtggcg ccggaggcct atgaaggcgg cacgattgcg
      541 ctggtgcagg agggcgactc cgtcaccatc gatgcgcaca agctgctgct gcagctcaac
      601 gtcggcgacg ccgagctggc tcagcgccgt gcggcctgga aggcaccggc accgcgctac
      661 acccgcggcg tgctggccaa gttcgcgaag aatgcttcca gcgccagctc gggtgccgtg
      721 ctcgacaaat tcgactgagt cagcggcgct tgcggcgcgt aggcaggccc agcgacgcga
      781 ggttgcgctt gcgcctttcc tcgcgcacgg cgtccacctt gtccatttcg cggcgcttgg
      841 tgtagccgct ggcatcggcc caagcccacc aggcaaccgc gcacgcaaag ggcgcgagca
      901 cgaggagcca ggaccagccg ccgacggggc cgaaatcggc gaccttcatc accagcagca
      961 aaacaccgat caccacgaac cacatggact gcactccccg cagcagcctg agccaagtca
     1021 agcgccgcta aactcacccc actagaatct ggccgaaact gtaaacgaac cctccgtgaa
     1081 ccgaaaggac gccatgaaac gcctgctgac cctgctcgcc gcggccctgc tcgccggacc
     1141 ggccctggcc gacgccaccg ccgacttggc gcagaagaag aactgcatgg cctgccatgc
     1201 catcgacaag aagctggtcg gccccgccta caaggacgtg gccgccaagt acgccggcca
     1261 gaaggacgcc gtggccaagc tgacgcaaaa ggtgctcaag ggcggctccg gcgcttgggg
     1321 cccggtgcct atgccagcca acacccaggt caacgaagcc gaggccaagc agctcgtgca
     1381 gtgggttctg agccagaagt aagactcagc attgaagacg aggaaacccg ccatcggcgg
     1441 gttttttcat gaccgggccg cctgtgcacc ggcaacgcat cgaaggaaag ccgccatcgg
     1501 gcgcagcgaa gcaaaccggc tcccagcgaa catgacaacg accaagccag tcaccccgac
     1561 gagctcagcg gccttcgagc ccgaattcgt gcagcgcctc aagaccatct tcgaggagag
     1621 catcagcttc aaccggctgc tcggcctgcg catcgacgag gtcgcaagcg agcgagtgac
     1681 ggcgcatctc gaaatgcgcc ccgagctgat cggtcatttc gcgcatcacc ggctgcatgg
     1741 cggcgtgatc agcgcgacgc tcgacgccat gggcggcttg gccgtgatgg ccgccatcgg
     1801 cgccagacac atggacgaag cgttagaggc gcggctgacg cgcttcggca agctgggcac
     1861 gatagacctg cgcatcgact atctgcgccc ggccacaggc ccgcgcttcg tggccgtggc
     1921 cgaggtgctg aggctgggca gccgcgtggc gacgacacgc atggaattcc tcgacgcgca
     1981 gggccggctg ctcagcacgg gcgcagccgc ctatatcgtg agctgaggcc ggcccatcag
     2041 cggccgacga gatccggccc aaaactcagc cctccagccc gctgcgcagc agtgctcgaa
     2101 ggcgcgcgac ctgctcgtcc gacatctcgc cgagcacgcc gcgtaggtgc ggcgggatgt
     2161 gcgccgccgc cgcttcgcgg tcgccgaaca gatagtgctc gaagatcgcg ctccaggcct
     2221 ggcgctgcgg ggagctcagc tgcctcagat tgagcaggct gtgcaagagg ccagccagcc
     2281 ccgaatcggt ccggcccgcg gtgccgatgg acccgttcca ccagtagttg acgaggacgt
     2341 tgaacttttc cagagacacc acgtgatgcc accacagcgt cgggatgaag atggcgtcgc
     2401 cgggcccgag ctcgccgaca tgggccgccg cgagcgcctt gcggaaccgc ggatgccgct
     2461 cgaaatccgg ggcctcgaaa tccaccaggc tgatgggcgt gttggtcggc gtgtagtcga
     2521 gcggaccgac gtagagattg gcgatctgct ccggcgagaa caaggtgaag cgccgccggc
     2581 cgctgacgac gcaggcgatg ttgtccgcct cgtcgaaatg cgccggcgtg gtgatcgcgt
     2641 tgcctagcca gatccgcggc tgtacggcgg catccagcag gcccaggcgg ttttcctcga
     2701 cgaagcccgg caggcagtcg gggatcaggg cgctttgcgc ggccaccgac gggggccgat
     2761 cgaacagcga atacctcagc acctgctcga tcaccttgga tagaggcagg cgattgcgca
     2821 ggaaattgaa gccgctcagg tccgcgttgt agaagatgcg gcccctctcc tcgggcggcg
     2881 tcatgatggc gtcgacctcg cggccgctgt ccagggcctt caggtagctg gacagcgccg
     2941 cggcagaaac ggcagcctcg cgtaccgcgg gccagtccct gaccaggccg cggaggatcg
     3001 ccggacggta ctgcgtgagg atgtcccggc ggatgcttgt ctcatcgaga cctgcgtact
     3061 cggggatggc ttgcgctgcg gcgtgcatgt ctgtggacag cggttgggga agcgcgcatc
     3121 ctcgcacagc cgcggtctgg accaactgat ctaggatggc aggattttgg cgagcacgtc
     3181 acgtcgctgc gcaacatgag tgctccccga acccggcgct tgcggcgctg cgcgcctcgc
     3241 ccctggcggg gcgcggatca gttggtctgg gccaactgat
//
LOCUS       DASUCY010000513         3032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1216930, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000513 DASUCY010000000
VERSION     DASUCY010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1216930"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1084)
                     /gene="thrC"
                     /locus_tag="VFV25_10095"
     CDS             complement(<1..1084)
                     /gene="thrC"
                     /locus_tag="VFV25_10095"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEY1393717.1"
                     /translation="MKYISTRGDQTPRRFCEILLEGLAPDGGLYLPVSYPKVDAATLA
                     RWRALPYAELAFELLSLYIDDIPPEDLRAIVRKTYTREVFGSDAIVPLKALEPGVQLE
                     ALSNGPTLAFKDMAMQLLGNLFEYELARRGETLNILGATSGDTGSAAEYAMRGKHGVN
                     VFMLSPHGRMSSFQQAQMFSLQDPNIHNLAVEGVFDDCQDIVKAVSNDLDFKRRYKIG
                     TVNSINWARLLAQVVYYFAGYFQATRSNDERVDFTVPSGNFGNVCAGHVARMMGLPIG
                     RLVVATNENDVLDEFFRTGTYRVRGGAETYETSSPSMDISKASNFERFVFDLLGRDAD
                     RTRQLFKTEIDQGGSFSLAPAEFARAD"
     gene            complement(1081..2403)
                     /locus_tag="VFV25_10100"
     CDS             complement(1081..2403)
                     /locus_tag="VFV25_10100"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEY1393718.1"
                     /translation="MKPIRIGLLGAGVVASGVVKVLERNQAEILRRAGRSIEVAAVAA
                     RDVEKARAALGNGIEFTQDFDALVVRPDIDIIVELIGGTSRSRELVLKALAAGKHVVT
                     ANKALLAVHGTEIFAQARERDVMVAFEAAVAGGIPIIKTLREGLTANRIEWIAGIING
                     TTNFILSEMRAKGLDFDVVLKEAQRLGYAEADPTFDIEGVDAAHKLAIMSAIAFGTPV
                     QFDKAYVEGITRLQATDINYAEQLGYRIKLLGITRQQAGGIELRVHPTLVPAKRLIAN
                     VEGAMNAVVVQGDAVGSTLYYGKGAGSEPTASAVVADLVDVTRLHTADPYHRVPHLAF
                     QPDALANTPILTMEQVVTSFYLRLRVADQAGVLANITRILADNDISIDAVLQRESKEV
                     GGNGASQTDLIILTHDTREGRMNAALAQMQALPTVLAPIVRIRKEELS"
     gene            complement(2412..>3032)
                     /locus_tag="VFV25_10105"
     CDS             complement(2412..>3032)
                     /locus_tag="VFV25_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEY1393719.1"
                     /translation="VIFADEIYDKTLYDGHTHTSIASLAGDVLCLSFNGLSKNYRSCG
                     YRAGWMVVSGEKRHAKDYIEGLNMLASMRLCPNTPGQLAIQTALGGYQSIKDLVAPGG
                     RLCRQRDLAYELLTQIPGVSVVKPKAALYMFPRLDPKLYPIQDDQQFAYELLAEEKVL
                     IVQGTGFNWPTPDHFRLVFLPNSDDLTDAIGRIARFLEHYRKRHSH"
BASE COUNT          474 a          971 c         1043 g          544 t
ORIGIN      
        1 cgtccgcgcg cgcgaactcg gccggtgcga ggctaaagct gccgccctgg tcgatctcgg
       61 tcttgaacag ctggcgagtg cggtcggcgt cgcggcccag caggtcgaag acgaagcgct
      121 cgaagttcga ggccttcgag atgtccatcg acgggctcga cgtctcgtag gtctcggcgc
      181 cgccgcgcac gcggtaggtg ccggtgcgga agaactcgtc gagcacgtcg ttctcgttgg
      241 tcgcgaccac cagcctgccg atgggcaggc ccatcatgcg cgccacgtgg ccggcgcaga
      301 cattgccgaa gttgcccgag ggcacggtga agtcgacgcg ctcgtcattg ctgcgcgtgg
      361 cctggaagta gccggcgaag tagtagacca cctgggccag caggcgcgcc cagttgatcg
      421 agttgacggt gccgatcttg tagcggcgct tgaagtccag gtcgttcgag acggccttga
      481 cgatgtcctg gcaatcgtcg aacacgccct cgacggcgag gttgtggatg ttcggatcct
      541 gcaggctgaa catctgcgcc tgctggaagg agctcatgcg gccgtgcggg ctcagcatga
      601 agacgttcac gccatgcttg ccgcgcatcg cgtactccgc cgcactcccg gtatcgcccg
      661 aggtggcccc aaggatgttg agcgtctcgc cgcggcgcgc cagctcgtac tcgaagagat
      721 tgcccagcag ctgcatggcc atgtccttga aggccagcgt cgggccgttg gacagggcct
      781 cgagctgcac gccgggctcc agtgccttca gcggcacgat ggcgtcgctg ccgaacacct
      841 cgcgggtgta ggtcttgcgc acgatggctc gcaggtcttc cggcgggatg tcgtcgatgt
      901 agagcgacag caactcgaac gccagttcag cgtatggcag agcgcgccag cgcgccagcg
      961 tggcagcgtc gaccttcggg tagctcaccg gcaagtagag gccgccatcg ggcgccaggc
     1021 cttcgagcag aatctcgcag aagcgccgcg gcgtctgatc gccgcgcgtg ctgatgtact
     1081 tcaactcagt tcttccttgc ggatgcgcac gatcggcgcg agcacggtgg gcagcgcctg
     1141 catctgcgcc agcgcggcat tcatgcggcc ttcccgcgtg tcgtgcgtga ggatgatgag
     1201 atcggtctgg ctggcgccgt ttccgccgac ttctttgctc tcgcgctgca gcaccgcgtc
     1261 gatggagatg tcgttgtcgg ccaggatgcg cgtgatgttg gccagaacgc cggcctggtc
     1321 ggccacgcgc aggcgcaggt agaacgaggt caccacctgc tccatcgtca ggatgggcgt
     1381 gttggcgagc gcatcgggct ggaaggccag atgcggcacg cggtgatacg ggtccgccgt
     1441 gtgcaggcgc gtcacatcga ccaggtcggc caccacggcg gacgccgtgg gctcggagcc
     1501 ggcgcccttg ccgtagtaca gcgtggagcc cacggcatcg ccctgcacca caacagcatt
     1561 catcgcgcct tcgacgttgg cgatcagccg cttggccggc accagggtgg gatgcacgcg
     1621 cagctcgatg ccgccggcct gctgccgcgt gatgcccagc agcttgatgc ggtagcccag
     1681 ctgctcggcg tagttgatgt cggtggcctg cagcctggtg atgccctcga cataggcctt
     1741 gtcgaactgc accggggtgc cgaaggcgat tgcgctcatg atggcgagct tgtgcgcggc
     1801 atccacgcct tcgatgtcga aggtcgggtc ggcctcggca tagcccaggc gctgggcctc
     1861 cttcagcacc acgtcgaagt ccaggccctt ggcccgcatc tccgacagga tgaagttggt
     1921 ggtgccgttg atgatgccgg cgatccactc gatgcggttc gccgtgagcc cctcacgcag
     1981 cgtcttgatg atggggatgc cgccggccac tgccgcctcg aaggcgacca tcacgtcgcg
     2041 ctcgcgggcc tgcgcgaaga tctcggtgcc gtgcacagcc agcagcgcct tgttggccgt
     2101 cactacatgc ttgccggcgg ccagcgcctt gagcaccagc tcgcgcgagc gcgaggtgcc
     2161 gccgatcagc tcgacgatga tgtcgatgtc aggacgcacc acgagggcgt cgaagtcctg
     2221 cgtgaactcg atcccgttcc ccaaggcggc gcgcgctttc tcgacgtccc gcgccgcaac
     2281 cgcggccacc tcgatactgc ggccggcgcg ccgcaggatt tcggcctggt tgcgctccag
     2341 caccttgacc acgccgctgg cgaccacgcc cgcgcccagc aggcctatgc gtatcggttt
     2401 cattgttgct ctcagtggga gtggcgcttg cggtagtgct cgaggaagcg ggcgatgcgg
     2461 ccgatcgcat ccgtcaaatc gtcggagttc ggcaggaaga ccaggcggaa gtgatcgggt
     2521 gtcggccagt tgaagccggt gccttgcacg atcaacacct tctcctcggc cagcagctcg
     2581 taggcgaact gctggtcgtc ctggatcgga tagagcttgg gatcgagcct ggggaacatg
     2641 tagagcgcgg ccttgggctt gacgacgctg acgcccggga tctgcgtgag cagctcgtag
     2701 gccaggtcgc gctggcgaca cagccggccg ccgggtgcca ccaggtcctt gatgctctgg
     2761 tagccgccca aggcggtctg gatcgccagc tgccccggcg tgttggggca caggcgcatc
     2821 gaggccagca tgttcaggcc ctcgatgtaa tccttggcat ggcgcttctc gcccgacacc
     2881 accatccagc cggcgcggta gccgcaggag cggtagttct tcgacaggcc gttgaagctg
     2941 aggcacagca cgtcgccggc cagcgaggcg atgctggtat gggtgtggcc gtcgtagagc
     3001 gtcttgtcgt agatctcgtc ggcgaagatg ac
//
LOCUS       DASUCY010000514         2212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_847351, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000514 DASUCY010000000
VERSION     DASUCY010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847351"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="VFV25_10110"
     CDS             complement(<1..729)
                     /locus_tag="VFV25_10110"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY1393720.1"
                     /translation="MMKKSLIALAALAAFAGAAQAQSGVNLYGIVDLGLRHSNNEGLS
                     KGSENQETAGMSQSRWGINVNEDMGGGLRTLVNLENRFSAQNGAIANAAQWSQSWVGI
                     QSSDFGQIRLGRQYNVLFDVMTTTYVPFKFSPYLEVFKPETIGHLSGGVTGLGSRNDN
                     MVKYLATLGAFTFEAQVSALQGDPTLVKSWGAAAKYGFGELALGGGYLEEKAMNGGKA
                     KAYNLGVGYTSGPLYLNASYAHSTF"
     gene            complement(983..1723)
                     /locus_tag="VFV25_10115"
     CDS             complement(983..1723)
                     /locus_tag="VFV25_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEY1393721.1"
                     /translation="MKAPEDDDSVEKTLLQRHASRYAAPEGLPQRITAALQREAAEAG
                     AAEAHQATHPTSPPTARPAVRRMNWLQALLMYGLGAATAWLAAGVLLVPPPQQPQADD
                     AAEVTASHVRSLMAEHLSDVASSDRHTVRPWFNGRLDYAPPVQDLADAGFPLVGGRLD
                     YIAGRPVAALVYRRGAHPINVFVWPAAAGAQANAKPAQLSRQGYALVSWAQAGMQFWA
                     VSDASPDELRDFSTRLQRKLAAAASPGG"
     gene            complement(1710..>2212)
                     /locus_tag="VFV25_10120"
     CDS             complement(1710..>2212)
                     /locus_tag="VFV25_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEY1393722.1"
                     /translation="ARWLLRDEHDAEDVVQEAYLRAFRFFAALRGADAKPWLLGIVRN
                     ACYDWLSQRSRREANHVEFDELQDSEAELQLAEPAATPEALLARRWEGRQVNEALASL
                     PPPLREVLILREIEELSYEDIARIAGIPIGTVMSRLSRGRARLRAALSQVLQEPLPTR
                     SRHEGA"
BASE COUNT          335 a          755 c          766 g          356 t
ORIGIN      
        1 gaaagtgctg tgcgcgtagg aggcgttcag gtacagcggg cccgaggtgt agcccacacc
       61 caggttgtag gccttggcct tgccgccgtt catcgccttc tcctccaggt agccgccacc
      121 caaggccagc tcgccgaagc cgtacttggc cgccgcgccc caggacttca ccagcgtcgg
      181 gtcgccctgc agcgcgctga cctgggcctc gaaagtaaag gcgcccaagg tggcgaggta
      241 cttgaccatg ttgtcgttgc gcgagcccag gcccgtcacg ccgcctgaca gatgcccgat
      301 ggtctcgggc ttgaagacct cgaggtaggg cgagaacttg aacggcacat aggtcgtggt
      361 catcacgtcg aacagcacgt tgtactggcg gcccaggcgg atctggccga agtcgctcga
      421 ctggatgccg acccaggact gcgaccactg ggcggcgttg gcgatggcgc cgttctgcgc
      481 cgagaagcgg ttctcgaggt tgaccagggt gcgcaggcca ccgcccatgt cctcgttgac
      541 gttgatgccc cagcggcttt gtgacatgcc ggcggtctcc tggttctcgc tgcccttgct
      601 tagcccctcg ttgttggagt ggcgcagccc caggtcgacg atgccgtaca ggttcacgcc
      661 cgattgggcc tgagcggccc cggcgaaggc cgccagggcg gcgagagcga tgagggattt
      721 cttcatcatg cgaggtctcc gttgacgttc ttgatttcgc gccggcgtgg ccgcgcgctg
      781 cggccattcg aacgaatgta ggcagcgggt agcgcacgca gaacgagggt taatccggtt
      841 ctgttgcgat ttgctaacgg atcgcgcgaa gctcctagga tgggttgtcg cgcgcgggac
      901 attgcagatg tctccctggc gacgctttcg cggcaaacca gcctcgccgg ccgatcggac
      961 cgaacgctgg agcgccctgc actcaaccgc cgggcgaagc tgcagcagcc agcttgcgct
     1021 gcagccgggt cgagaagtcc cgcaactcgt cggggctggc atccgagacg gcccagaact
     1081 gcatgccggc ctgcgcccag ctcacgagcg catagccctg gcggctcagc tgggcgggct
     1141 tggcgttcgc ttgtgcgccg gccgcggccg gccacacgaa gacattgatc ggatgcgcgc
     1201 cgcgccgata gaccagcgcc gccaccggcc gcccggcgat gtagtccagc cggccaccga
     1261 ccagcggaaa gcccgcgtcg gccaggtcct gcaccggcgg cgcgtagtcg agcctgccgt
     1321 tgaaccaggg gcgcaccgtg tggcggtcgc tcgatgcgac atcggacaga tgctcggcca
     1381 tcagcgagcg cacatggctg gccgtgacct cggccgcatc gtccgcctgc ggttgctgcg
     1441 gcggcggcac gagcaggacg ccggccgcca gccaggccgt cgccgcgccc aggccgtaca
     1501 tcagcagggc ctgcagccag ttcatccggc gcacggccgg acgagcggtc ggaggagagg
     1561 tcggatgcgt ggcctgatgc gcctcggccg caccagcctc agcagcttcg cgctgcagcg
     1621 cggcggtgat gcgctggggc agcccctcgg gcgcggcata gcggctcgca tgccgctgca
     1681 gcaaggtctt ctcgacgctg tcgtcgtcct caggcgcctt catggcggct ccttgttggc
     1741 aaaggctcct gcaggacctg gctcaaggcg gcgcgcagcc gggcgcggcc gcgcgacagg
     1801 cgcgacatca cggtgccgat ggggatgccg gcgatgcggg caatgtcctc ataactcagc
     1861 tcctcgatct cgcgcaggat cagcacctcg cgcagcggcg gcggcaggct ggccagcgcc
     1921 tcgttgacct gccggccctc ccagcgccgc gccagcagcg cctcgggcgt tgcagcgggc
     1981 tcggccagct gcagttcggc ttcgctgtcc tgcagttcgt cgaactcgac atggttcgct
     2041 tcgcggcggc tgcgctggct cagccagtcg tagcaggcat tgcgcacgat gcccagcagc
     2101 cagggcttgg catcggcgcc gcgcagcgcg gcgaagaagc gaaaggcgcg caaataggcc
     2161 tcctgcacca cgtcctcggc gtcgtgctcg tcgcgcagca gccagcgcgc ca
//
LOCUS       DASUCY010000515         4924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_622345, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000515 DASUCY010000000
VERSION     DASUCY010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4924
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622345"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="VFV25_10125"
     CDS             complement(<1..844)
                     /locus_tag="VFV25_10125"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEY1393723.1"
                     /translation="MPVIKRAWFTGQTPALAGAQSAASWVALPPQSEEAAGPDASAAP
                     SRYRSVWISDVHLGTPGCQAHALLDFLREVESDHLFLVGDIVDGWQLRRSWYWPQAHN
                     DVVQKLLRKARKGTRVMFIPGNHDEFARKYLNHNFGGIEVVEDWTHTTADGRRLWITH
                     GDLFDGVIQCAKWLAYVGDSAYEFTLKLNRHFNSLRARLGLPYWSLSKYLKLKVKRAV
                     NFIGDFELAVAREARKRGVHGVVCGHIHHAELREIDGVLYANDGDWVESLTALVEHAD
                     GRLEI"
     gene            complement(917..2269)
                     /locus_tag="VFV25_10130"
     CDS             complement(917..2269)
                     /locus_tag="VFV25_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY1393724.1"
                     /translation="MKSLRVRLLVSLLAMVAGAALLVGGLTYRSVLHETEALFDYQLR
                     QMALSLRDQGAITGQAANALADEQFDFVVQIWSLDGTEIYASREHAALPQRAVLGFAD
                     VAVDGQGWRTFTVASRERVIQVAQPDNIRQQLAADAALHSIAPLLALGPLMALGVLWL
                     VTASLAPLRRLVAAVRERGAAALQPLPQADLPGEVAPLVAELNTLLSRLEQAFAAQRA
                     FVSDAAHELRSPLTALKLQLRTLQQAPDEPSREQAAGRLGQGIDRATRLVAQLLTLAR
                     NEPGARPAALVPVDLTEAVRQAVAETVPLAHARGSSLALETEAPVMVLGEADGLRVLA
                     RNLADNALRYSPPGSTVSLRIESDGAGHAELLVDDSGPGIPPPQRERVFDRFSRRAGA
                     THGSTQGAADTDSDAEQGSGLGLAIVHSVADRFGAQVMLEDSPLGGLRVRSRFRAAPA
                     "
     gene            complement(2266..2937)
                     /locus_tag="VFV25_10135"
     CDS             complement(2266..2937)
                     /locus_tag="VFV25_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY1393725.1"
                     /translation="MRLLLIEDDRMIGESVRQALRHEGHAVDWVRDVRAAQGTLATEQ
                     FDCVLLDLGLPDGDGLDVLRTLRSRRDATPVILLTARDALEDRVAGLDAGADDYLVKP
                     FEFDELAARMRAVLRRRAGRADTLLAHEGVTLDPATRQVMLRGEPVLLSAREFAVLEA
                     LMSRPGAILSRAQLEDRLYGWGDAIESNAISVYIHQLRRKLGADFIRNVRGVGYFVGK
                     PGGEA"
     gene            3031..3348
                     /locus_tag="VFV25_10140"
     CDS             3031..3348
                     /locus_tag="VFV25_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HEY1393726.1"
                     /translation="MTEKIHAAAVTTKANVYFDGRCVSHSIELAEGKKASVGVILPSE
                     LVFTTGAAEVMEGVAGACEVRLKGESGWTRYAAGQSFSVPAQSSFEIRVVGEPYHYIC
                     RFS"
     gene            3460..4254
                     /locus_tag="VFV25_10145"
     CDS             3460..4254
                     /locus_tag="VFV25_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEY1393727.1"
                     /translation="MQALTSAIRVAGLALALLAGVAHAADMLDEVKQRGTLRIAVEGT
                     YPPFNFRDEQGKLTGFDVEIAEAIAAKLGVKPEFTTTEWSGILAGLQAGKYDVIVNQV
                     AATDKRRETFDFSDPYVVSSPQLIVRANETRKLATPADLKGKTIGVGQGSNYADQARG
                     FEGAEVKVYPGAPEYLQDLATGRIDVALNDSLLIPYLQQKTKLPIKAGAPIGKVESNA
                     IPFRKGNPKFKAAIDKALADLKADGSFAKISDKWFKRDVSKPPAAQ"
     gene            4404..>4924
                     /locus_tag="VFV25_10150"
     CDS             4404..>4924
                     /locus_tag="VFV25_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEY1393728.1"
                     /translation="MDLASLFSYAAPLMLKGTGYTLLFASASMLLGLALGAAIALLRV
                     LALPGLSQVAAVFVSAMRGTPLLVQVFIVYYGLPSIGIELSPIAAGILALTLNVAAYR
                     SESLRGAVSGIAQGQWLAGTSLGLTRVQTLRYVIGPQALRSAVPSLSNSLISLIKDTS
                     LVSVIAVTELMLAT"
BASE COUNT          791 a         1744 c         1667 g          722 t
ORIGIN      
        1 ggatctccag ccggccgtcg gcatgctcga ccagggccgt caggctctcg acccagtcgc
       61 cgtcgttggc atagagcacg ccgtcgatct cgcgcagctc ggcatggtgg atatggccgc
      121 agaccacccc gtgcacgccg cgcttgcgcg cctcgcgcgc cacggcgagc tcgaagtcgc
      181 cgatgaagtt gacggcgcgc ttgaccttga gcttgaggta cttggacagc gaccagtagg
      241 gcaggcccag ccgcgcgcgc agcgaattga aatgccggtt cagcttgagc gtgaactcat
      301 aggcggagtc gcccacatag gccagccatt tggcgcactg gatgacgccg tcgaacaggt
      361 cgccatgggt gatccacaga cgccggccgt ccgcggtcgt atgcgtccag tcctccacca
      421 cctcgatgcc gccgaaattg tggttcaggt atttgcgcgc gaactcgtcg tggttgccgg
      481 ggatgaacat cacccgcgta cccttgcgcg ccttgcgcag cagcttctgc accacgtcgt
      541 tgtgggcctg cggccagtac cagctgcggc gcagctgcca gccgtcgacg atgtcaccga
      601 ccaggaagag gtggtcgctc tcgacttccc tcaggaaatc gagcagcgca tgggcctggc
      661 agccgggcgt accgagatgg acgtcggaga tccagacact gcgatagcgg ctgggcgcgg
      721 ccgaggcgtc ggggccggcc gcctcctcgg actgcggcgg cagcgccacc cagctcgcag
      781 cggactgggc gcccgccagc gcgggcgttt gcccggtgaa ccatgctcgc ttgatgacag
      841 gcatccatcg catggaccca tatgctcgcc cgccggcgtg acgccgccgc gtcggccgcg
      901 tgaagattcg atgacatcaa gccggcgccg cccgaaacct gctgcgcacg cgcagcccgc
      961 cgagcggcga gtcctccagc atcacctggg cgccgaagcg gtcggccacg ctgtgcacga
     1021 tggccaggcc caggccgctg ccctgctcgg cgtcgctgtc agtgtcggca gcgccttggg
     1081 tactgccgtg cgtcgcgccg gcacggcggg agaagcggtc gaagacgcgt tcgcgctggg
     1141 gcggcgggat gccggggccg gagtcgtcca ccagcagctc ggcatgcccg gcgccgtcgg
     1201 attcgatgcg caggctcact gtcgagcccg gcggcgaata gcgcagcgca ttgtcggcga
     1261 ggttgcgcgc cagcacacgc aggccgtcgg cctcgcccag caccatcacc ggcgcctcgg
     1321 tttccaacgc caacgagctg ccgcgcgcat gggccaacgg caccgtctcg gccacggcct
     1381 gacgcaccgc ctcggtcagg tcgaccggca ccagcgccgc cggccgtgcg cccggctcgt
     1441 tgcgcgccag cgtgagcagc tgcgcaacca gccgcgtcgc acggtctatg ccctggccga
     1501 gccggcccgc ggcctgctcg cgcgagggct cgtccggcgc ctgctgcagc gtgcgcagct
     1561 gcagcttgag cgccgtgagc ggcgagcgca gctcgtgagc cgcatcggac acgaaggcgc
     1621 gctgggcggc gaaggcctgc tccagtctgc tcagcagcgt gttgagctcg gccaccagtg
     1681 gcgccacctc gcccggcagg tcggcctggg gcagcggctg cagcgcggcg gcgccgcgct
     1741 cacgcacggc cgccaccagg cgccgcagcg gcgccaacga tgccgtgacc aaccacagca
     1801 cgcccagcgc catcaagggc cccagggcca gcagcggcgc gatgctgtgc agggccgcgt
     1861 cggccgccag ctgctggcgg atgttgtcgg gctgcgccac ctggatcacg cgctcgcgcg
     1921 aggccaccgt gaaggtgcgc cagccctggc cgtcgaccgc gacatcggca aagcccagca
     1981 ccgcgcgctg gggcagcgcc gcatgctcgc gcgaggcgta gatctcggtg ccgtccaggc
     2041 tccagatctg cacaacgaaa tcgaactgct cgtcggccag cgcattggca gcctggcccg
     2101 tgatggcgcc ctggtcgcgc agcgacagcg ccatctggcg cagctggtag tcgaacaggg
     2161 cctcggtctc gtgcagcacg ctgcgatagg tgagcccgcc caccagcagg gccgcgcccg
     2221 ccaccatggc gagcagcgag accagcagcc ttacgcgcag ggacttcatg cctcgccgcc
     2281 cggcttgccg acgaaatagc ccacgccgcg cacgttccgg atgaagtcgg cgcccaactt
     2341 gcggcgcagc tggtggatgt agacggagat cgcattgctc tcgatggcat cgccccagcc
     2401 gtagagccgg tcttccagct gggcgcgcga caggatcgcg ccgggccgcg acatcagggc
     2461 ctccagcacc gcgaactcgc gcgccgacag cagcacgggc tcgccgcgca gcatgacttg
     2521 gcgcgtcgcc ggatccagcg tcacgccctc gtgcgccagc agcgtatcgg cgcggccggc
     2581 gcggcgcctc agtacggcgc gcatgcgagc ggccagctcg tcgaactcga agggcttgac
     2641 gagatagtcg tcggcgccag cgtccagccc ggccacgcgg tcttccagcg catcgcgggc
     2701 ggtgagcagg atcaccggcg tcgcgtcgcg ccgcgagcgc agcgtgcgca gcacgtccag
     2761 gccgtcgcca tcgggcaggc cgaggtcgag cagcacgcag tcgaactgct cggtggccag
     2821 cgtgccctgg gcggcgcgca catcgcgcac ccagtccacc gcgtggcctt cgtggcgcaa
     2881 ggcctggcgc acgctctcgc cgatcatgcg gtcgtcttcg atcagcagca gacgcatcgg
     2941 gtcttcaggg ctgtatggga cctgaaagga tacgacgccg cgccgaagtc ggagaattgc
     3001 ggtttcgaca cacagccaag gaccgccacc atgaccgaga agatccatgc cgccgccgtc
     3061 accaccaagg ccaacgtcta tttcgacggc cgctgcgtct cgcacagcat cgagctggcc
     3121 gagggcaaga aggcgagcgt gggcgtgatc ctgccctccg agctggtctt cacgaccggc
     3181 gcggccgagg tcatggaagg cgtagccggc gcctgcgagg tcaggctcaa gggcgagagc
     3241 ggctggacgc gctatgcggc gggccaaagc ttctcggtgc cggcgcaatc cagcttcgag
     3301 atccgtgtgg tcggcgagcc ctaccactac atctgccgct tcagttaaac cggccacccc
     3361 gcggtgggca tgctcgcccg gcgcatatcc atgctcggcg catgcaggac ggcggtggca
     3421 acatcgcgcc tcaccaacat cttcctggga gcttcgttca tgcaagcttt gacatccgcc
     3481 atacgcgttg ccggcctggc gctggccctg ctggccggcg tggcccacgc tgccgacatg
     3541 ctcgacgagg tgaagcagcg cggcacgctg cgcatcgccg tcgagggcac ctacccgccc
     3601 ttcaacttcc gcgacgagca gggcaagctg accggcttcg acgtcgagat cgccgaggcg
     3661 atcgccgcca agctgggcgt gaagcccgag ttcacgacca ccgaatggag cggcatcctg
     3721 gccgggctgc aggcaggcaa gtacgacgtc atcgtcaatc aggttgccgc caccgacaag
     3781 cgccgcgaga ccttcgactt cagcgatccc tatgtcgtct ccagcccgca gctgatcgtg
     3841 cgcgccaacg agacgcgcaa gctggccacg ccggccgacc tcaagggcaa gacgataggc
     3901 gtgggccagg gcagcaacta tgccgaccag gcgcgcggct tcgaaggcgc cgaggtcaag
     3961 gtctatcccg gcgcgccgga gtatctgcag gacctggcga ccggccgcat cgacgtggcg
     4021 ctgaacgaca gcctgctgat cccctatctg cagcagaaga ccaagctgcc gatcaaggcc
     4081 ggcgcgccga tcggcaaggt ggagagcaac gccatcccct tccgcaaggg caatccgaag
     4141 ttcaaggcgg cgatcgacaa ggccttggcc gacctcaagg ccgatggcag ctttgccaag
     4201 atctcggaca aatggttcaa gcgtgacgtc agcaagccac ctgccgcgca gtaaaccgct
     4261 cgcttagcat tgcaccgccc ctggcgatcc caggggcggt ttcgtttctc aaggaaacgc
     4321 cgggccgccc caagtttcct tgacccccgc ggggggcggg ccgggcgcag cccggacctg
     4381 ggggcactat ggagggggtc accatggatc tcgcgtcgct gttcagctac gccgcgccgc
     4441 tgatgctcaa gggcaccggc tacacgctgc tgttcgcctc tgcatcgatg ttgctcgggc
     4501 tggccctggg cgccgcgatc gcgctgctgc gggtgctggc gctgccgggc ctgagccagg
     4561 tggcggccgt ctttgtgagc gcgatgcgcg gcacaccgct cctggtgcag gtcttcatcg
     4621 tctactacgg cctgcccagc atcggcatcg agttatcgcc catcgcggcc ggcatcctgg
     4681 cgctgacgct caatgtcgcg gcctatcggt ccgaaagtct gcgcggcgcc gtgtccggca
     4741 tagcccaggg ccagtggctg gccggcacca gcctgggcct gacgcgcgtg cagacgctgc
     4801 gctatgtcat cggccctcag gccttgcgca gcgcggtgcc cagcctgtcg aacagcctga
     4861 tcagcctgat caaggacacc tcgctggtct cggtgatcgc cgtgaccgag ctgatgctgg
     4921 cgac
//
LOCUS       DASUCY010000516         4979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1377272, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000516 DASUCY010000000
VERSION     DASUCY010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4979
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1377272"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1936
                     /locus_tag="VFV25_10155"
     CDS             <1..1936
                     /locus_tag="VFV25_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEY1393729.1"
                     /translation="GVFAVAGYVTLTCAAVYLAGVLFLVLNKANPRQAHFGSIVHYWD
                     LYAGDTQLRRKLQLAIGVSGLGLLVLLPAGLAAAARPRRALHGDARFANSAEIDRAGL
                     TGSDGQPGILIGRHRGRFLSLPGQLSVMLSAPTRSGKGVGVVIPNLLNWPDSVVVLDI
                     KGENYDITAGYRAAHGQAVYAFSPFDEEARCHRWNPLTAVRSSPLHRVGDLLTIGQVF
                     FPNDGGGTPSEAFFNDQARNLFLGLGLVLLETPGLPRSIGEMLRQSSGKGRSLKDHLT
                     ALITQRRDEGHPLTDECIDALQRLLSNSENTLSSVVATFNAPLTIFADAVVDAATSAD
                     DFGLEDVRRRRMSVYVRIPPNRLANARPLLNLFFSQLVSLNTQALPKQDPTLKLQCLL
                     VNDEFAAMGRVGVITNAAAFLAGYNLRLLTVVQAMSQLDAVYGDKEARTFATNHGLQI
                     LFAPREQRDADEYSAMLGHFTERATSHGRSRSFSGHGHSTVSRNESEQRRALLLPQEF
                     KELGSERLVVIFENCKPILGEKIRYWRDQTFRSRLLPAPAVPRMDMDLHLARVQRRWR
                     YADDELEPGTSLSVEALAYDLSQLPDLADAPPERIAESVIDFFSRAQPDHPGGAIEPV
                     ADEDGVLIAAPSTIERAELT"
     gene            1947..2600
                     /locus_tag="VFV25_10160"
     CDS             1947..2600
                     /locus_tag="VFV25_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY1393730.1"
                     /translation="MRASIALTPFVPWLLLLASCSSPPKPPTVDESQKRPVNSQTAVE
                     LQVCKNDLQNTRLLATESGRLAETTAATLANLAARQQILAAMQGPAGQQAQANSIFTV
                     RFEFGSTRVVIPADIAASLVEDAKAAPLVLLRGRTDGTSDAPAESRIARDRAAAVRDY
                     LVAAGVDPARIRATYQPAGDHVADNASAGGRGLNRRVEIELYRALPVALTPTTVAQP"
     gene            2634..3782
                     /locus_tag="VFV25_10165"
     CDS             2634..3782
                     /locus_tag="VFV25_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="HEY1393731.1"
                     /translation="MDADNAPTRTPAPAQGGAVEPANRATVAKERFQTPHDSFGERYQ
                     LRDPFAEITHRADTFPEMVAKAEHLGSTRFFAVTEDGKRAPIQKVDGQWVRDGQRGPQ
                     RPASPERPLDPMAARDEVPEASSVVPMPGVAKTPAQAEQTDAKAIARIDAQAERAALV
                     ARLEAALQERYIIRRAPVTVGDVSIGRTEYRFRGDTSRVAFTESTFRLATDTNSPSVA
                     RSMVDVAEARNWKALRVSGNEDFTRMVWLEASVRGVKTLGYVPNPGDLELLKREREAR
                     LVNRIEPAREAGSGAAAAVTEKASGRGGGRKTVLAAIEAVLVAKRVPEKQRAAVMAAA
                     TEKLAQRMREGRAPKVKVYDKAAPQRPVIVPTPELQRSRDWAAPASVR"
     gene            3793..>4979
                     /locus_tag="VFV25_10170"
     CDS             3793..>4979
                     /locus_tag="VFV25_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1173 domain-containing protein"
                     /protein_id="HEY1393732.1"
                     /translation="METSIHGTTAPSATYEVGGRRFEIGSPDFAEAIAEAHTSRLRPR
                     CTCQLEGVEMYVARLAGPSGGYIVKRMPDTGSLHAPGCPSYEPPAEFSGLGQVLGSAI
                     IEDPATGETTLTLDFPLTQVPGRAAPPPAGGDGDSVSSSGTKLSLRGLLHYLWDQAEL
                     TRWHPGFAGRRNWAMVRRHLLQAAEHKIARGDALHARLYVPEPFSVDERDAINARRLA
                     QWQYAVAAPGKPQHLMLLIGEVKEIVPARYGFKALVKHMPDQAFAIDEQLYRRLGRRF
                     EPQLALWGAADDIHMVMIATFGLSSTGIPAIHELCLMPVSRQWLPVEDAFEKQLIDRL
                     VNDARSFIKGLRYNLGAASALASATLTDCEGAAHLLFVVPANVEDSGRYLQVCDPSTP
                     VWL"
BASE COUNT          795 a         1836 c         1647 g          701 t
ORIGIN      
        1 cggcgtgttc gcggtggccg gctacgtcac gctgacctgc gccgcggtgt acttggccgg
       61 cgtgctgttc ctggtactga acaaggccaa cccgcggcag gcgcacttcg gcagcatcgt
      121 ccactattgg gacctgtacg ccggcgatac ccagctgcgc aggaagctcc agctcgcgat
      181 cggcgtctcg gggctcggcc ttttggtcct gctccccgcg ggtctggcgg cagcggcccg
      241 cccgcgccgc gccctgcatg gtgacgcccg gttcgcgaac agcgccgaga tcgatcgcgc
      301 ggggctcacg ggcagtgatg gccagccggg catcctcata gggcgccacc gggggcgctt
      361 cctgtccctg cccggccagc tctcggtgat gctgtccgcc cccacccgca gcggcaaggg
      421 cgtgggcgtc gtcatcccca acctgctgaa ctggcccgac tcggtcgtgg tgctcgacat
      481 caagggagag aactacgaca tcacggccgg ctatcgggcc gcgcatggcc aggccgtcta
      541 tgccttctcg cccttcgacg aggaagcacg ctgccaccgc tggaacccgc tgaccgccgt
      601 tcgttccagc ccgctgcacc gcgtgggcga cctgctcacc atcggccagg tcttcttccc
      661 caacgacggc ggcggcaccc cgtcggaagc cttcttcaac gaccaggcgc gcaacttgtt
      721 cctgggcctg ggcctcgtcc tcctcgaaac ccccgggctg ccgcgcagca tcggcgagat
      781 gctgcgccag tcctccggca agggccgatc gctcaaggat cacctgaccg cgctgatcac
      841 gcagcgccgc gacgaaggcc atccgttgac ggacgaatgc atcgacgcgc tgcagcgcct
      901 gctgtcgaac tcggagaaca cgctgtccag cgtcgtcgcc accttcaatg cgccgctgac
      961 gatctttgcg gatgcggtgg tggatgccgc caccagcgcc gacgatttcg ggctcgagga
     1021 cgtgcgccgc cgccgcatgt cggtctacgt gcgcatcccg cccaaccggc tggccaatgc
     1081 gcggccgctg ctgaacctgt tcttctcgca gctcgtcagc ctgaacacgc aggccctgcc
     1141 gaagcaggat ccgacgctca agctgcaatg cctgctggtc aacgacgagt tcgcggccat
     1201 gggccgcgtg ggcgtcatca ccaacgccgc cgccttcctg gccggctaca acctgcgcct
     1261 gctgaccgtg gtgcaggcca tgtcgcagct cgatgccgtc tacggcgaca aggaggcccg
     1321 cacctttgcc accaaccacg gcctgcagat ccttttcgcg ccgcgcgagc agcgcgacgc
     1381 cgacgagtac agcgcgatgc tcggccactt caccgagcgc gccacctcgc acgggcgcag
     1441 ccggtccttc agcggccacg ggcacagcac ggtcagccgc aacgaaagcg agcagcgccg
     1501 tgcgctgctg ctgccgcagg agttcaagga actgggcagc gaacgcctgg tggtgatctt
     1561 cgagaactgc aagcccatcc tcggcgagaa gatccgctac tggcgcgacc agaccttcag
     1621 gtcgcgcctg ctgccggcgc cggccgtgcc gcgcatggac atggacctgc acctggcgcg
     1681 cgtgcagcgg cgctggcgct atgccgacga cgagctcgag cccggcacca gcctgagcgt
     1741 cgaggcgctg gcctatgacc tcagtcagct gcccgacctc gccgacgcgc cgccggagcg
     1801 gatcgccgag agcgtgatcg atttcttcag cagggcccag ccggaccacc ccggtggtgc
     1861 gatcgagcct gtcgccgatg aagacggcgt gctcatcgcc gccccctcca cgatcgaacg
     1921 cgccgaactg acctaggagc aacgccatgc gagcgagcat cgccctcacg cctttcgtcc
     1981 cctggctcct gctgctggcc tcctgcagct cgccaccgaa gccgccgacc gtggacgagt
     2041 cgcagaagcg gccggtgaat tcccagacgg ccgtcgagct gcaggtctgc aagaacgacc
     2101 tgcagaacac gcgcctgctg gccaccgaat ccggccggct cgccgaaacc actgcggcta
     2161 cgctggccaa cctggcggcc cgccagcaga tcctggccgc catgcaaggg ccggccggcc
     2221 agcaggccca ggccaacagc atcttcacgg tgcgtttcga gttcggcagc acgcgtgtcg
     2281 tgatcccggc cgacatcgca gcgtccctgg tcgaggacgc caaggcggcg ccgctcgtgc
     2341 tgctgcgcgg ccgcaccgac ggcaccagcg acgcccccgc cgaaagccgc atcgcgcgcg
     2401 acagggccgc cgcggtgcgc gactacctgg tggccgcggg cgtggacccg gcccgcatcc
     2461 gtgccaccta ccagccggcc ggcgaccacg tggccgacaa cgccagcgcc gggggccgcg
     2521 gcctgaaccg tcgcgtcgag atcgagctgt accgggcact gccggtcgcg ctgaccccga
     2581 ccaccgtcgc ccagccctga agggccacca cacaccatct tcgcgaggcc gccatggatg
     2641 cagacaacgc tcccacccgc accccggcac cagcccaagg cggcgccgtc gagcccgcca
     2701 accgcgccac cgtcgccaag gaacggttcc agacgccgca cgactcgttc ggcgagcgct
     2761 accagctgcg cgaccccttc gccgagatca cccacagggc cgacaccttc cccgagatgg
     2821 tggccaaagc cgagcatctg ggcagcaccc gcttcttcgc ggtgaccgag gacggcaagc
     2881 gcgcgccgat ccagaaggtc gatggccaat gggtgaggga tgggcagcgc ggcccgcagc
     2941 gccctgcctc gcccgaacgc ccgctggatc ccatggcggc gcgcgacgag gtgcccgagg
     3001 cgtcgagcgt cgtcccaatg cccggcgtcg cgaagacgcc tgcgcaagca gaacagaccg
     3061 atgccaaggc catcgccagg atcgacgcgc aggccgagcg tgccgcgctc gtggcccgcc
     3121 tcgaggctgc gctgcaggag cgctacatca tcaggcgcgc cccggtgacg gtgggcgacg
     3181 tgagcatcgg ccgcaccgag taccgcttcc gcggcgacac ctcgcgcgtg gccttcaccg
     3241 aatcgacctt ccgcctggcc accgatacca acagcccgtc ggttgcccgg tcgatggtcg
     3301 acgtggccga ggcacgcaac tggaaagcgc tgcgcgtgtc cggcaacgag gacttcacgc
     3361 gcatggtctg gttggaagcc tcggtgcgcg gcgtcaagac tctgggctat gtgccgaacc
     3421 cgggcgacct ggaactgctc aagcgcgagc gcgaggcgcg cctggtcaac cgtatcgagc
     3481 ctgcccgcga agccggcagc ggcgccgcgg ctgcggtgac cgagaaggcc tccggccgcg
     3541 gcggcgggcg caagaccgtg ctggccgcga tcgaagcggt gctggtcgcc aagcgagtgc
     3601 ccgagaagca gcgcgccgcc gtgatggccg ccgcgaccga gaagctcgcg cagcggatgc
     3661 gcgagggccg ggcgccgaag gtcaaggtct acgataaagc ggcgccccag cgaccggtca
     3721 tcgtgcccac gcccgagctg cagcgctcgc gcgattgggc ggcgcccgcc tccgtgcggt
     3781 gaggcaattc cggtggaaac gtcgatccac ggcaccacgg caccgtcagc gacctatgag
     3841 gtcggcggcc ggcgcttcga gatcggctcg cctgacttcg cggaggccat cgcggaggcc
     3901 cacacatctc gcctgcggcc ccgctgcacg tgccagctcg aaggcgtcga gatgtacgtc
     3961 gcccgcctag cgggtccgag cgggggctac atcgtcaagc gcatgccgga caccggcagc
     4021 ctacacgcgc ctggctgccc gtcctacgag ccgccggccg agttctccgg cctggggcag
     4081 gtgctgggca gcgcgatcat cgaggacccg gccaccggcg agaccacgct gacgctggac
     4141 ttccctctga cccaggtccc cggtcgcgcc gcgccgccgc ccgccggcgg cgacggcgac
     4201 agcgtgtcgt cgagcggcac caaactctcg ctgcgcggcc tgctgcatta cctgtgggac
     4261 caggccgagc tgacgcgctg gcatccgggg ttcgcgggca ggcgaaactg ggccatggtc
     4321 cgaaggcact tgctgcaggc cgccgagcac aagatcgcgc gcggcgatgc actgcacgcc
     4381 cggctctacg tgccggagcc cttctccgtc gatgagcgcg acgcgatcaa cgcccggcgc
     4441 ctggcgcagt ggcagtacgc cgtggccgcg cccgggaaac cgcaacacct gatgctgctc
     4501 atcggcgagg tcaaggagat cgtgccggca cgctacggct tcaaggcgct cgtcaagcac
     4561 atgcccgacc aggccttcgc gatcgacgag cagctctacc ggcggctcgg gcgtcgcttc
     4621 gagcctcagc tggcgctgtg gggtgccgcc gatgacatcc acatggtgat gatcgccacc
     4681 ttcggcttga gcagcaccgg catcccggcg atccacgagc tgtgcctgat gccggtgtcg
     4741 cggcaatggc tgccggtcga agacgcgttc gaaaagcagc tcatcgaccg gctcgtgaat
     4801 gacgcacgtt cgttcatcaa gggacttcgg tacaacctcg gcgcggcgag cgcgctggcc
     4861 agcgcaacgc tgaccgattg cgagggcgct gcgcacctgc tcttcgtcgt tcctgcgaac
     4921 gttgaggaca gcgggcggta tcttcaggtg tgcgaccctt cgacgccggt atggctctg
//
LOCUS       DASUCY010000517         4039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_863032, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000517 DASUCY010000000
VERSION     DASUCY010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863032"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..927)
                     /locus_tag="VFV25_10175"
     CDS             complement(19..927)
                     /locus_tag="VFV25_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY1393733.1"
                     /translation="MDRLKQIESFVAVATKGSLTAAANAEGVAPAVIGRRIDALEERL
                     GVKLLVRTTRRITLTHEGSAFLEDSQRLLADLANAEASVSAGGVKASGHLRLTAPAGF
                     GRRHVAPLVPRFVELHPEVSISLNLSDRVVDIVNEGIDCAVRVGDLPDSSLVSLRLAD
                     NRRLCVATPAYLSAHGTPQHPNDLNRHRCLTLSSEASQTRGWAFLVDGETTYLRPPAR
                     LDCSDGQVLHDWCLAGLGVAWRSTWEVEGEIRAGQLVSLLDAYAAPPNGIYAVFPQRK
                     HLPLRVRLWIDFLKHSYGDADYWSAH"
     gene            1028..1723
                     /locus_tag="VFV25_10180"
     CDS             1028..1723
                     /locus_tag="VFV25_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEY1393734.1"
                     /translation="MKPNASPQAVLFDAYGTLFDVHSVASLAEQFFAGQGERLSQLWR
                     DKQIEYTRLVSMSGTERYRPFWELTRAGLRFAAQRLKLDLTPEREERLMNQYRALAAF
                     PENKGVLQALKKLGVPTGILSNGDPEMLAVAVKSAGFDSLLDHVLSADGVRRYKTDPA
                     VYALGPQALGLQPKQILFVSSNGWDAIGAAWFGYTTLWVNRAGAPLEQLDTEPTRIGS
                     SLRDVLSFFGAAS"
     gene            1759..3954
                     /locus_tag="VFV25_10185"
     CDS             1759..3954
                     /locus_tag="VFV25_10185"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HEY1393735.1"
                     /translation="MSTRTTIHRLQVANDLYRFIEDQVLPATGVSSASFWQGFDAIVH
                     DLAPKNTALLAERDRLQVELDAWHKAHPGPIADMPAYRAFLEKIGYLVPQPKGARATT
                     ANVDAELAVQAGPQLVVPILNARYALNAANARWGSLYDALYGTDAIPETGGAEKDAKG
                     YNPVRGARVIEFARGVLDQAAPLAAHSHKDATGYRVEGGKLVVSLRNGSTTGLEDAAQ
                     FVGHQGDAAAPSSVLLKHHGLHIDIRIDRNSAIGKTDPAGVSDVVLEAALSTILDLED
                     SVAAVDAQDKVLAYSNWLGILQGTLTEQVAKAGKTFTRGLNPDRQYTGPDGKPVALQG
                     RSLMFVRNVGHLMTNPAILYGDGREIPEGIMDAVITTTIALVDLKRRANSRTGSIYIV
                     KPKMHGSKEVAFASELFGRVEALLGIPANTVKLGIMDEERRTSVNLQACIAEAAARVA
                     FINTGFLDRTGDEMHTAMQAGAMFRKGDMKTSAWIQAYEKSNVLVGLACGLRGRAQIG
                     KGMWAMPDLMAAMLEQKIAHPKSGANTAWVPSPTAATLHALHYHQVDVSAVQRELEKI
                     DAQTDPVQQRDDILNRLLQIPVVARPQWSAAEIQQELDNNTQGILGYVVRWIDQGVGC
                     SKVPDIHNVGLMEDRATLRISSQHIANWLHHGVVTRNQVDATFQRMAAVVDKQNAGDP
                     LYKNMAGNFGSSAAYMAAQDLVFKGLEQPSGYTEPLLHAWRLKVKAGQA"
BASE COUNT          725 a         1399 c         1310 g          605 t
ORIGIN      
        1 ttttcttttc ttcgcggctc agtgcgccga ccagtagtcc gcgtcgccgt agctgtgctt
       61 caggaagtcg atccatagcc gcacgcgcag cggcagatgc ttgcgctgcg gaaacaccgc
      121 gtagatgccg ttgggcggcg cggcataggc gtcgagcagg ctgaccagtt gcccggccct
      181 gatctcgccc tcgacctccc aggtcgagcg ccaggcgacg cccaggccgg ccaggcacca
      241 gtcgtgcagc acctggccgt ccgaacagtc gagtcgcgca ggcgggcgca ggtacgtggt
      301 ttcgccatcg accaagaagg cccagccgcg cgtctggctg gcttcagagc tgagcgtgag
      361 gcagcgatgc cggttcaggt cgttgggatg ctggggcgtg ccatgcgccg acagataggc
      421 cggcgtggcc acgcacaggc ggcggttgtc ggccaggcgc aagctcacca ggctggagtc
      481 cggcaggtcg ccgacacgca cggcgcaatc gatgccctcg ttgacgatgt cgacgacacg
      541 atcggagagg ttgagcgaaa tcgagacctc gggatgcagc tcgacgaagc gcggcaccag
      601 cggcgctacg tggcggcgcc cgaaaccggc cggcgccgtg agccgcagat ggccgctggc
      661 cttgacgccg ccggcgctga cgctggcctc ggcattggcc agatcggcga gcaggcgttg
      721 actgtcctcc aggaaggcgc tgccctcgtg cgtgagcgtg atgcgccttg tggtgcgcac
      781 cagcagcttc acgcccaggc gctcctccag cgcgtcgatg cgccgcccga tcacggccgg
      841 cgccacgccc tcggcattgg cggcggccgt caggctaccc ttggtggcca cagcgacaaa
      901 ggattcgatc tgtttcagac ggtccatgag ccgcagatta catacgaatc gagcaaagat
      961 caacgcgcaa cgggttcatt tatcgctact tcagtcagca ctagagtgcg aagcttcagt
     1021 tcgcaccatg aagcccaacg cctccccgca agcggtcctc ttcgacgcct atggcacgct
     1081 gttcgacgtg catagcgtgg cgagcctggc cgagcagttc ttcgccggcc agggcgagcg
     1141 cctgagccag ctgtggcgcg acaagcagat cgagtacacg cggctcgtct cgatgagcgg
     1201 taccgagcgt taccgcccct tctgggagct cacgcgcgcc gggctgcgct ttgccgcgca
     1261 gcgcctcaag ctcgacctga cgcccgagcg cgaagagcgg ctgatgaacc agtaccgcgc
     1321 gctggcggcc ttccccgaga acaagggcgt gctgcaggcg ctgaagaagc tgggcgtgcc
     1381 caccggcatc ctgagcaacg gcgatcccga gatgctcgcg gtggcagtca agagcgccgg
     1441 cttcgacagc ctgctcgacc acgtgctgag cgccgatggc gtgcgccgct acaagaccga
     1501 tccggccgtc tacgcgctcg ggccccaggc gttggggctg cagcccaagc agatcctctt
     1561 cgtgtccagc aatggctggg acgcgatcgg cgcggcctgg ttcggctaca cgacgctatg
     1621 ggtcaaccgc gccggtgcgc cgctggagca gctcgacacc gagcccacgc gcatcggcag
     1681 cagcctgcgc gacgtgctga gcttcttcgg cgccgcgtcc tgaatcttgg ttttcacttt
     1741 catccggagc ctgccaccat gagcacacgc accaccatcc accgactcca ggtcgccaac
     1801 gatctgtatc gcttcatcga ggaccaggtg ctgccggcca ccggtgtcag cagcgccagc
     1861 ttctggcagg gcttcgacgc catcgtgcac gacctggcgc ccaagaacac cgcgctgctc
     1921 gccgagcgtg atcgccttca ggtcgagctc gatgcctggc acaaggccca tcccgggccc
     1981 atcgccgaca tgccggccta tcgcgccttc ctggagaaga tcggctatct cgtgccgcag
     2041 cccaagggcg cgcgcgcgac caccgccaat gtcgacgccg agctcgccgt gcaggccggc
     2101 ccgcagctcg tcgtgcccat cctcaacgca cgctatgcac tcaacgccgc caatgcgcgc
     2161 tggggctcgc tctatgacgc cctctacggc accgacgcga ttcccgaaac gggcggcgcc
     2221 gaaaaggacg ctaagggtta caacccggtg cgcggcgccc gcgtgatcga gttcgcgcgc
     2281 ggcgtgctcg accaggccgc gccgctggcc gcacattcgc acaaggacgc gaccggctac
     2341 cgcgtcgaag gcggcaagct cgtcgtctca ctgaggaatg gctctaccac cggtctggag
     2401 gacgccgcgc aattcgtcgg ccatcaaggc gatgcggcgg cgccttcttc ggtgctgctc
     2461 aagcaccacg gcctgcacat cgacatccgc atcgaccgca acagcgccat cggcaagacc
     2521 gatccggccg gcgtcagcga cgtggtactg gaagcggcgc tgtccaccat cctcgacctg
     2581 gaagactcgg tggctgcggt cgacgcgcag gacaaggtgc tggcctacag caactggctg
     2641 ggcatcctgc agggcacgct gaccgagcag gtcgccaagg ccggcaagac cttcacgcgc
     2701 ggcctcaacc ccgaccgcca gtacaccggc cccgacggca aaccggtggc gctgcaaggc
     2761 cgctcgctga tgttcgtgcg caatgtcggc cacctgatga ccaacccggc catcctgtac
     2821 ggcgacggcc gcgagatccc cgaggggatc atggacgcgg tcatcacgac gactatcgca
     2881 ctggtcgacc tgaagcggcg cgcgaactcg cgcaccggca gcatctacat cgtcaaaccc
     2941 aagatgcacg gctccaagga agtggccttc gccagcgagc tgttcggccg cgtcgaggcc
     3001 ttgctgggca tccccgccaa cacggtgaag ctgggcatca tggacgagga gcgccgcacc
     3061 agcgtcaatc tgcaggcctg catcgccgaa gcggccgcgc gcgtggcctt catcaacacc
     3121 ggcttcctcg accgcaccgg cgacgagatg cacaccgcga tgcaggccgg cgcgatgttc
     3181 cgcaagggcg atatgaagac cagcgcctgg atccaggcct acgagaagag caatgtgctg
     3241 gtggggctgg cgtgcgggct gcgcggccgt gcgcagatcg gcaagggtat gtgggccatg
     3301 cccgacctga tggccgcgat gctggagcag aagatcgccc atcccaagtc gggcgccaac
     3361 accgcctggg tgccttcgcc caccgcggcg acgttgcatg cgctgcatta ccaccaggtc
     3421 gacgtgagcg cggtgcagcg cgagctcgag aagatcgatg cccaaaccga tccggtccag
     3481 cagcgcgacg acatactcaa ccgcctgctg cagatccccg tggtggccag gccgcagtgg
     3541 tcggccgccg aaatccagca ggagctggac aacaacaccc agggcatcct cggctacgtg
     3601 gtgcgctgga tcgaccaggg cgtaggctgc tccaaggtgc ccgacatcca caacgtcgga
     3661 ttgatggaag accgcgcgac gctgcgcatt tccagccagc acatcgccaa ctggctgcac
     3721 cacggcgtcg tgacgcggaa ccaagtcgat gccaccttcc agcgcatggc cgcggtggtc
     3781 gacaagcaga acgccggcga cccgctgtac aagaacatgg ccggcaactt cggcagctcg
     3841 gccgcctaca tggccgcgca ggacctggtg ttcaaggggc tggagcagcc gagcggctac
     3901 accgagccgc tgctgcacgc ctggcgcctc aaggtgaagg ccgggcaggc ataggcgaat
     3961 agctcccaat gaaaacggcg cctgcgaggc gccgtttcct ttcgctgatg acccggcccc
     4021 ccccctgggg tgggcaaag
//
LOCUS       DASUCY010000518         5546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1554050, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000518 DASUCY010000000
VERSION     DASUCY010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 5546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5546
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1554050"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1313)
                     /locus_tag="VFV25_10190"
     CDS             complement(<1..1313)
                     /locus_tag="VFV25_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY1393736.1"
                     /translation="MKRVAVVGSGISGLAAAWLLSKPGAGASVTLYEAGDYFGGHAHT
                     VDVSLRDASGRPVTQGVDTGFLVLNERTYPSLLKLFAELGVELAPSDMSFSVQVPGPS
                     GIGLEWSGSDLSSVFAQRRNLLRPAFWSMLAEVLRFNRLATAIAESGSDEQLAQPLGE
                     FLDWHRFGTAFRDWYFLPMIGCIWSCPTGQMLHFPVATLIRFCHNHGLIQLRNRPQWY
                     TVRGGARCYVEKMLAGIADARLNCPVRAVRRVGGAAAILVATDAASEVYDEVVFACHS
                     DQALTLLGEDATAVERELLGAIRYHRNRALLHTDSRLLPQRRLAWAAWNYERPASLGR
                     EQTAVCLHYWLNRIQPLPFDQPLLVSLNPAREPREDSVLGEYDYSHPVFDLAAIAAQR
                     RLPEIQGRRHSWFCGAWTGYGFHEDGLNSAQAIVQALRDQAQAERA"
     gene            complement(1642..2199)
                     /locus_tag="VFV25_10195"
     CDS             complement(1642..2199)
                     /locus_tag="VFV25_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEY1393737.1"
                     /translation="MKSAIRLLFAVLTCLGAGAASAAPSCPSLLAHEFPRLQDEKPQD
                     LCQYAGKVVLVVNTASYCGYTPQYKSLEALYEKYRERGLVVLGFPTNDFGAQEPGKNG
                     EIAAFCENTYKVKFPMFAKSTVKGAQANVLIAELAQRSGQAPRWNFHKYLISRDGQKV
                     LSTSTTVDPLDPAFVQQVEKLLEVR"
     gene            2389..3258
                     /locus_tag="VFV25_10200"
     CDS             2389..3258
                     /locus_tag="VFV25_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEY1393738.1"
                     /translation="MLYPELFKQLEAVRWNMDKDIPWAQFDAAKLSEEQAQTIKMNAI
                     TEWAALPATEMFLRDNKDDSDFSAFMSVWFFEEQKHSLVLMEYLRRFRPDLVPSEQEL
                     HEVRFEFDPAPALETLMLHFCGEIRLNHWYRRAAEWHTEPVIKAIYETLARDEARHGG
                     AYLRYMKRALQKFGDEAKAAFAKVGVLMASARRTAQALHPTNLHVNEKLFPRDTIQSR
                     LPDPQWLEEWLDKQINFDAVWENKVVERILHNLSLLMERSFVSVQELNRYRKELIASI
                     AAAAQPPAGAQPA"
     gene            complement(3259..3936)
                     /locus_tag="VFV25_10205"
     CDS             complement(3259..3936)
                     /locus_tag="VFV25_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEY1393739.1"
                     /translation="MLKLYGFPLSNYHNKVKLALLEKGVPFEEVTIDKVSREDWLAHS
                     PLGKVPYIENEQGAALCESQAIIEYIEARWPTPALLPADPWAAAKLRELNLFLELHLE
                     LVARQLYGPAFFGLPPLPEKFTERVRAQLEKNIAAFKRLAKFAPYVGGDSFTMADCSA
                     YCHLPLVAMASRATYGVDLLAAADVDWKSYVKLIDSRSTAQKVAAERKADQGRAARMG
                     AALAKAA"
     gene            4020..4511
                     /gene="rfaE2"
                     /locus_tag="VFV25_10210"
     CDS             4020..4511
                     /gene="rfaE2"
                     /locus_tag="VFV25_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HEY1393740.1"
                     /translation="MDQPPALPRFFDKLCPRAALAQRLAALPRPVVFTNGVFDILHRG
                     HVSYLAQARALGASLVIGLNSDVSARQLGKGPDRPLNAEVDRAAVLAALESVGLVTLF
                     DEPTPLELLKLVRPDIYVKGGDYDIETLAETPLVRSWGGRSLALPFVEGYSTTALVKR
                     IRG"
     gene            4605..4883
                     /locus_tag="VFV25_10215"
     CDS             4605..4883
                     /locus_tag="VFV25_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEY1393741.1"
                     /translation="MKYIVPPAAFGLITIAASSLAFAKADCKAYPKAEWMPQAEAIAK
                     IEAQGFTINKFKADGNCYEIYGKNKAGKKVEIYFDVKTLEPVKSEVEQ"
     gene            4886..5416
                     /locus_tag="VFV25_10220"
     CDS             4886..5416
                     /locus_tag="VFV25_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEY1393742.1"
                     /translation="MREPRDAKLAPDGRILVWDAAVRLMHLLLAGLVVSDLVRDDGDR
                     LHRALGYAAAAVVLARLAWALLSRRGPVAPSVSATLAYLRARTPRRLDHDPLGLWMVW
                     LLWLLVLLLALTGWMSRLDAFWGDERIQQLHAWLADAMLVAVALHLMGVGVMSWRWRE
                     NLAASMIHGRKRAEKR"
BASE COUNT          966 a         1839 c         1803 g          938 t
ORIGIN      
        1 gctctttcgg cctgcgcctg atcgcgcagg gcctgcacga tggcctgcgc cgagttgagg
       61 ccgtcttcat gaaagccgta gccggtccag gcaccgcaga accagctgtg gcgtcgcccc
      121 tggatttcgg gcagcctgcg ctgcgcggcg atcgcggcga ggtcgaacac cggatggctg
      181 tagtcgtatt cgcccagcac gctgtcttcc cgcggctcgc gcgccgggtt cagcgagacg
      241 agcaagggct ggtcgaaagg caggggctgg atacggttga gccagtaatg caggcagacg
      301 gcggtttgct cgcgaccgag gctggccggg cgttcgtagt tccaggccgc ccaggctagt
      361 cggcgctgcg gcagcaggcg gctgtcggta tgcagcaatg cgcggttgcg gtgatagcga
      421 atcgcaccca gcagttcgcg ctcgacggcg gtcgcatcct cgccgagcag ggtcaatgcc
      481 tgatcgctat ggcaggcaaa gaccacctcg tcgtagacct cgctggccgc atccgtggcc
      541 accaagatcg cggccgcccc gcccacccgg cgcaccgcgc gcaccggaca gttcagccgc
      601 gcatcggcga tgcccgccag catcttctcc acatagcagc gcgccccgcc gcgcaccgtg
      661 taccactgcg gccgattcct cagctggatc agcccgtggt tgtgacagaa acggatcaga
      721 gtggcgaccg gaaagtgcag catctgcccg gtcggacagg accagatgca gccgatcatc
      781 ggcaggaaat accagtcgcg aaaggcggtg ccgaagcgat gccagtcaag aaattcgccc
      841 aggggctggg ccagttgttc gtcgctcccg ctttctgcaa tggccgtggc gagacgattg
      901 aagcgcaaga cctcggccag catggaccag aaggcggggc gcagcaggtt gcgtcgttgt
      961 gcaaacacgc tgctcaggtc gctgccgctc cattccaggc cgatgccgct cggacccggc
     1021 acctgcaccg agaacgacat gtccgaaggc gccagctcga cccccagctc ggcgaacagc
     1081 ttcagcaggc tcgggtaggt gcgctcgttc agcaccagaa agccggtgtc gacaccctgc
     1141 gtgacaggcc ggccgctcgc atcccgcaag ctcacgtcga ccgtgtgcgc atggccgccg
     1201 aaatagtcac ccgcttcgta tagcgtgacc gaagcgccgg cgcccggctt gctcagcagc
     1261 caagcggcgg ccagccccga aatgccggag ccgacgacgg cgacgcgctt caagcgcggc
     1321 ccttcgtcga ctcgggaagc aggcccgcgg cggcgtagac cacgccgccg cggcacgaga
     1381 gaaacgccgc gaagcgcccc agagcaaagg agggagggag ggaggaggag aagcgctcta
     1441 gggttgcagc cggacgatcc ggaaggcttc gcagcagaaa gtgtgtaggg cggagaacga
     1501 ccttgttctg tctgccttgc cggcatcgct tgatgccgac acagctcatt cgaggggcct
     1561 ggcgcagcgg agttccctga tgcatgctgc tttacttttt ttgaccttct tttgggctac
     1621 agccgtcttc aacgacgctc ttcatctgac ttcgaggagc ttttccactt gctggacgaa
     1681 ggccggatcg agcgggtcaa ccgtcgtgga ggtactcagc actttctggc cgtcgcgcga
     1741 gatcaggtac ttgtggaaat tccagcgcgg tgcctggcca ctgcgttgcg ccagctcggc
     1801 gatcaggaca ttggcctggg cgcccttgac ggtggacttc gcgaacatcg ggaacttcac
     1861 cttgtaggtg ttctcgcaga aggccgcgat ctcgccgttc ttgcccggct cctgggcacc
     1921 gaagtcattg gtcgggaagc ccagcaccac gaggccgcgc tcgcggtact tctcgtacaa
     1981 ggcctccagg ctcttgtatt gcggtgtgta gccgcagtag ctcgcggtat tgaccaccag
     2041 caccaccttg ccggcgtatt ggcaaagatc ctggggcttt tcgtcctgca ggcgcgggaa
     2101 ttcgtgagcc agcaggctgg ggcagctcgg cgcggcgctc gccgccccgg cacccaggca
     2161 ggtcagcaca gcgaacagca ggcgaatggc agacttcatc atgatcccta tccttgcggt
     2221 gtaagaggcc tttcgagcgg catgccgggc atccctccgc ggagtttcgc tccagctcaa
     2281 tatacgcatc ggcaccgggt tttgcatggg cacaaggagc cccacagccg tgacacggtt
     2341 gtgacacctg ccccgcctac aatgcctggt tactccgcga ggtccgccat gctttacccc
     2401 gaactcttca agcaactcga agccgtgcgc tggaacatgg acaaggacat cccttgggcc
     2461 cagttcgacg ccgccaagct gtccgaagag caggcccaga ccatcaagat gaacgccatc
     2521 acagagtggg cggcgctgcc ggccaccgag atgttcctgc gcgacaacaa ggacgacagc
     2581 gacttctcgg ccttcatgag cgtgtggttc ttcgaggagc aaaagcattc gctggtgctg
     2641 atggaatacc tgcgccgctt ccgccccgac ctcgtgccca gcgagcagga gctgcacgag
     2701 gtccgcttcg agttcgaccc ggccccggcg ctggagacgc tgatgctgca tttctgcggc
     2761 gagatccgcc tcaaccactg gtaccgccgc gccgccgaat ggcacaccga gccggtgatc
     2821 aaggccatct atgaaaccct ggcgcgcgac gaggcccgcc atggcggcgc ctacctgcgc
     2881 tacatgaagc gcgccctgca gaagttcggc gacgaggcca aggccgcctt cgccaaggtg
     2941 ggcgtgctga tggccagcgc ccgccgcacc gcgcaggcgc tgcacccgac caatctgcac
     3001 gtcaacgaga agctcttccc gcgcgacacc atccagagcc gcctgcccga cccgcaatgg
     3061 ctggaggagt ggctggacaa gcagatcaac ttcgacgccg tgtgggagaa caaggtcgtc
     3121 gagcgcatcc tgcacaacct gagcctgctg atggagcgca gtttcgtgag cgttcaggag
     3181 ctcaaccgct accgcaagga gctgatcgcg tcgatcgctg ccgctgccca gccgccggcc
     3241 ggggctcagc cggcctgatc aggcggcctt cgcaagagcc gcacccatgc gcgcggcgcg
     3301 cccttggtcg gccttgcgct ctgcggccac cttctgcgcc gtggaacggc tgtcgatcag
     3361 cttcacatag ctcttccagt cgacgtcggc cgcagccagc aggtcgacgc cgtaggtcgc
     3421 gcggctggcc atcgcgacca gcggcagatg gcaataggcc gagcaatcgg ccatcgtgaa
     3481 gctgtcgccg ccgacatagg gcgcgaactt ggcgagccgc ttgaaggcgg cgatgttctt
     3541 ttcgagctgg gcgcgcacgc gctcggtgaa tttctcgggc agcggcggca ggccgaagaa
     3601 ggccgggcca tagagctggc gcgccaccag ctccagatgc agctcgagga agaggttgag
     3661 ttcgcgcagc ttggccgcgg cccagggatc ggccggcagc agggccggcg tgggccagcg
     3721 tgcctcgatg tactcgatga tggcctggct ctcgcacagc gcggcgccct gctcgttctc
     3781 gatgtacggc accttgccca gcggcgaatg ggccagccag tcttcgcggc tcaccttgtc
     3841 gatcgtcacc tcctcgaagg gaacgccctt ttccagcagg gccagcttga ccttgttgtg
     3901 atagttcgaa agcgggaagc cgtagagctt gagcatggat gtctcctggg aaccttgcca
     3961 ctgaagtcta ggcgcgccgg cgtccatcca gcgttacgca ggcgacaatg ccgcgcatca
     4021 tggatcagcc gcccgccctg cccaggtttt tcgacaagct ctgcccgcgc gccgcattgg
     4081 cgcagcgcct ggccgcgctg ccgcgccccg tggtgttcac caacggcgtg ttcgacatcc
     4141 tgcaccgcgg ccacgtcagc tatctggccc aggcacgcgc cttgggtgcc agcctcgtga
     4201 tcggcctcaa cagcgacgtc tcggcccgtc agctgggcaa ggggcccgac cgccccttga
     4261 acgccgaggt cgaccgcgcc gccgtgctgg ccgcgctgga gagcgtgggc ctcgtgacgc
     4321 tgttcgacga gcccacgccg ctggaactgc tcaagctggt gcgccccgac atctatgtga
     4381 agggcggcga ctacgacatc gaaaccctgg ccgagacgcc gctggtgcgc agctggggcg
     4441 gccgctcgct ggccctgccc tttgtcgagg gctactcgac gacggcactg gtgaaacgca
     4501 tccgcggctg aacgcccgac gcaccattgt ggattaaatg cgaattattc gcatctgagt
     4561 gctagacttt gcggcgtcat caacgtctca ctcagggagc cccgatgaaa tacatcgttc
     4621 cgccagccgc cttcggtctc atcaccatcg ccgcttcgtc actcgccttc gccaaggccg
     4681 attgcaaggc ttatcccaag gccgaatgga tgccgcaggc cgaggccatc gcgaagatcg
     4741 aagcccaggg cttcacgatc aacaagttca aggccgacgg caactgctac gagatttacg
     4801 gcaagaacaa ggccggcaaa aaggtcgaga tctacttcga cgtgaagact ctggagcccg
     4861 tgaagtccga agtcgagcag tagacatgcg cgagccgcgc gacgcaaagc tcgcgcccga
     4921 cggccgcatc ctggtctggg atgcggccgt tcgcctgatg cacttgctgc tggcggggct
     4981 ggtcgtctcc gacctggtcc gagatgacgg cgaccggctg caccgcgcgc taggctatgc
     5041 ggccgcagcg gtggtcttgg cgcgcctcgc atgggcgctg ctgtcgcggc gcgggccggt
     5101 cgccccgtcg gtgtccgcca cgctggccta tctgcgtgcc cgcacgccgc gcaggctgga
     5161 tcacgacccc ctgggcctgt ggatggtctg gctgctgtgg ctgttggtgc tgctgctggc
     5221 cctcaccggc tggatgagcc ggctcgacgc cttctggggc gacgagcgca tccagcagct
     5281 ccatgcctgg ctggccgacg cgatgctggt ggccgtggct ttgcatctga tgggcgtagg
     5341 cgtgatgagc tggcgctggc gcgagaacct cgcggcctcc atgatccacg ggcgcaagcg
     5401 cgccgagaag cgctaaggat gccggtttgc gcgccccggc gagcacgcgc agaatcgtct
     5461 ggccggattg gagccagccc catgagcagc ctctctgaag acgattcagc gccgcgctgg
     5521 aagcacgacg gcgtgcgcgt gatccc
//
LOCUS       DASUCY010000519         3564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_301033, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000519 DASUCY010000000
VERSION     DASUCY010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301033"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VFV25_10225"
     CDS             complement(<1..331)
                     /locus_tag="VFV25_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY1393743.1"
                     /translation="MANIYETQLDKNAANFVALSPVSFVERSAEVFGDLAAVIHGARR
                     YSWAQTRERSARLAAALHALGIRRGSTVSVMLPNTPEMVEAHYSVPALNAVLNTLNTR
                     LDAALLAW"
     regulatory      439..532
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     regulatory      542..654
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            696..1811
                     /gene="gcvT"
                     /locus_tag="VFV25_10230"
     CDS             696..1811
                     /gene="gcvT"
                     /locus_tag="VFV25_10230"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEY1393744.1"
                     /translation="MSAELLKTPLHGLHLELGAKMVEFGGYDMPVNYPGGIIAEHKHC
                     RESASLFDVSHMGQLRLVGDAAAQALETLVPVDVIDLGVGKQRYAFFTNAAGGILDDL
                     MITRRANDLFVVVNAACKATDTQHLITHIGHRCQVVPLPERALLALQGPKAVTALTRL
                     NPEVAGLTFMTGGSFALNGVECFITRSGYTGEDGFEISVPAEAAVALAKALLQQPEVK
                     PAGLGARDTLRLEAGLCLYGHDINTTTTPVEAGLTWAIQKVRRPGGARAGHYPGAAAI
                     EAQLGTTPPVKRVGLLGLERVPVREGAPLLDARGHKLGHVTSGTLSPSLGQPIAMAYL
                     AANHALPDHEVWAEVRGKGQPMRVRAMPFTPHRYHRG"
     gene            1868..2245
                     /gene="gcvH"
                     /locus_tag="VFV25_10235"
     CDS             1868..2245
                     /gene="gcvH"
                     /locus_tag="VFV25_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEY1393745.1"
                     /translation="MTTMYTPDHEWINIEDHEAAVVGITLHAQDALGDVVFVDLPEVG
                     KTYKKGEVCGVVESVKAAADVFCPVSGEVVEVNEALRNDPALANSDPMGEGWFFKIHV
                     TNMAEFDALMDGPSYDVLLKKSA"
     gene            2304..>3564
                     /locus_tag="VFV25_10240"
     CDS             2304..>3564
                     /locus_tag="VFV25_10240"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012838237.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HEY1393746.1"
                     /translation="MLMSTVQPLDALENSCEFVARHIGVDDADEQHMLSVIGAASRRA
                     MIEAIVPGSIARAQTMKLPAAVTEAGALAELKAIAAKNKLLKSFIGQGYYGTLTPAVI
                     LRNILENPAWYTAYTPYQAEISQGRMEALVNFQTMVCDLTGMPIANASMLDEATAAAE
                     AMTLAKRSVKSKSEVFVIGGDAHPQTIEVIQTRAKPLGIKLVVANSAAEWDAALSGEF
                     FGALIQYPASSGWLMDWSAEVKKIHDKQAAAIFATDLLALTLLVPPGEWNADIVVGST
                     QRFGMPMGAGGPHAAFLACRDDYKRSMPGRLVGVSVDAHGQPAYRLALQTREQHIRRE
                     KATSNICTAQVLPAVVASMYAVYHGPQGLKRIAQRVASYAAILARGLRQLGYGATHHD
                     TAFDTISLKTDAETAALQDKARKAGMNL"
BASE COUNT          627 a         1263 c         1146 g          528 t
ORIGIN      
        1 gccaggccaa gagcgccgca tccaggcgcg tgttgagcgt gttgagcacg gcattcagcg
       61 ccggcaccga gtagtgcgcc tccaccatct cgggcgtgtt gggcagcatc acgctgaccg
      121 tgctgccccg ccggatgccc agcgcgtgca gcgcggcggc caggcgcgcc gaacgctcgc
      181 gcgtctgggc ccagctgtag cggcgcgcac cgtggatcac ggccgcgagg tcgccgaaca
      241 cctcggcact gcgctccaca aagctgaccg gcgacaaggc gacgaagttg gccgcgttct
      301 tgtcgagctg ggtctcgtag atgttcgcca tcttgctcct ccagttttcc gcagcgctgc
      361 cgcaaggcga cggcccatca cggcattgtg cgaccgaaaa cgccgcccat gatgcgcccg
      421 gtcgaagctc ggataaactc cccgaagtca ggagagcgtg cgccggatgg cgtaccgccg
      481 aaggcgcagg gggttgcccc gaacgctcag gcaaaaggac tggcaggcgt ctcgacgaga
      541 gacgttcctc actggagaga ggttggccct tgagccgatc caccgaaggg gcaagcggaa
      601 tgccggcaca ccggatctgc gaatctctca ggtaccaagg acagcgaggg cttccccacc
      661 ggaaggtggt tgttcgatag ccctcggagc ctgatatgtc cgccgaattg ctcaagaccc
      721 cgctgcatgg cctgcacctc gaactcggcg ccaagatggt cgagttcggc ggctacgaca
      781 tgcccgtcaa ctaccccggc ggcatcatcg ccgagcacaa gcactgccgc gagtcggctt
      841 cgctgttcga cgtgtcgcac atgggccagt tgcgcctggt cggcgacgcc gcggcccagg
      901 cgctcgagac cttggtgccg gtggatgtaa tcgatctggg cgtgggcaag cagcgctacg
      961 ccttcttcac caatgccgcc ggcggcattc tcgacgacct gatgatcacg cggcgagcga
     1021 acgacctgtt cgtggtcgtc aacgctgcct gcaaggccac cgacactcag cacctgatca
     1081 cccacatcgg ccaccgctgc caggtcgtgc cgctgcccga gcgcgcgctg ctggcgctgc
     1141 agggtcccaa ggccgtcacg gcgctgacgc ggctgaaccc cgaggtcgcc gggctcacct
     1201 tcatgaccgg cggcagcttc gcgctcaacg gcgtcgagtg cttcatcacc cgctcaggct
     1261 ataccggcga ggacggcttc gagatctccg tacccgccga ggccgcggtg gcgctggcca
     1321 aggccctgct gcagcagccc gaggtcaagc ccgccggcct gggcgcgcgc gacacgttgc
     1381 gtctggaggc ggggctttgc ctctatggcc acgacatcaa caccaccacg acgccggtcg
     1441 aggccggcct cacctgggcg atccagaagg tgcgacgccc gggtggcgcc cgcgccggcc
     1501 attaccccgg cgccgcggcg atcgaagccc agcttggcac cacccctccc gtcaagcgcg
     1561 tgggcctgct gggcctggag cgcgtgccgg tgcgcgaagg cgcgccgctg ctcgatgcac
     1621 gcggccacaa gctgggccat gtcaccagcg gcacgctgtc gccttcgctc ggccagccga
     1681 tcgcgatggc ctacctggcc gccaaccatg ccctgcccga tcacgaggtc tgggccgagg
     1741 tgcgcggcaa aggccagccc atgcgtgtcc gcgccatgcc cttcacgccg caccgctatc
     1801 accgcggctg agcgcttccg cgcgaatcac cgtccctctt atcccgaatc cccccaagga
     1861 gctcccgatg accaccatgt acacccctga tcacgaatgg atcaacatcg aagaccacga
     1921 agcagcggtc gtcggcatca cgctgcacgc gcaggatgcg ctgggcgacg tggtcttcgt
     1981 cgatctgccc gaggttggca agacctacaa gaagggcgag gtctgcggcg tggtcgagtc
     2041 ggtcaaggcg gccgccgacg tgttctgccc ggtgagcggc gaggtcgtcg aggtcaacga
     2101 ggcgctgcgc aacgacccgg cgctggccaa cagcgacccg atgggcgaag gctggttctt
     2161 caagatccac gtcaccaaca tggccgagtt cgatgcgctg atggacggcc cctcctatga
     2221 cgtgctgctg aagaagagcg cctgaacgcg ctcctcccct ctcctctcgt tccaagatct
     2281 acctccgtcg tatcggagca accatgctca tgtccaccgt tcagccgcta gacgccctcg
     2341 agaactcttg cgaattcgtc gcacgccaca tcggcgtcga cgacgccgac gagcagcaca
     2401 tgctcagcgt gatcggcgcc gcgtcgcgcc gcgcgatgat cgaggccatc gtgccgggca
     2461 gcatcgcccg cgcccagacg atgaagctgc cggctgcggt gacggaggcc ggcgcgctgg
     2521 ccgagctcaa ggccatcgcg gcgaagaaca agctgctcaa gagcttcatc gggcagggtt
     2581 actacggcac cctgacgccg gccgtcatcc tgcgcaacat cctcgagaac cccgcctggt
     2641 acacggccta cacaccctat caggccgaga tctcgcaggg ccgcatggag gcgctggtca
     2701 acttccagac catggtgtgc gacctcaccg gcatgcccat cgccaacgcg tccatgctcg
     2761 acgaggccac ggccgccgcc gaggcgatga cgctcgccaa gcgcagcgtc aagagcaaga
     2821 gcgaggtctt cgtgatcgga ggcgacgcgc atccgcagac catcgaggtc atccaaacgc
     2881 gcgccaagcc gctgggcatc aagctggtgg tcgccaactc ggcagcggaa tgggatgcag
     2941 ccctgagcgg cgagttcttc ggcgcgctga tccagtaccc ggccagcagc ggctggctga
     3001 tggactggag cgccgaggtc aagaagatcc acgacaagca ggccgccgcc atcttcgcca
     3061 ccgacctgct ggcgctgacg ctgctcgtgc cgcccggcga gtggaacgcc gacatcgtgg
     3121 tcggcagcac gcagcgcttc ggcatgccca tgggcgcggg cggtccgcac gccgccttcc
     3181 tggcctgccg cgacgactac aagcgctcga tgccgggccg cctggtcggc gtcagcgtgg
     3241 acgcgcatgg ccagccggcc taccggctcg cgctgcagac gcgcgagcag cacatccgcc
     3301 gcgagaaggc cacctccaac atctgcacgg cgcaggtgct gccggccgtg gtggcgagca
     3361 tgtatgccgt atatcacggg ccgcaagggc ttaagcgcat cgcgcagcgg gtcgcctcct
     3421 acgcagcgat ccttgcgcgc ggactcaggc agctgggcta tggcgccacc caccacgaca
     3481 cggccttcga caccatcagc ctgaagaccg atgccgaaac ggcagcgctg caagacaagg
     3541 cccgcaaggc cggcatgaac ctgc
//
LOCUS       DASUCY010000520         2516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1393487, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000520 DASUCY010000000
VERSION     DASUCY010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1393487"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VFV25_10245"
     CDS             <1..849
                     /locus_tag="VFV25_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393747.1"
                     /translation="LKAAFGYAPLAVVLSAIAVSLTQTLLAVTDSFSDFMLNGYQAQV
                     AATIGSLAGVLAAGAAGTVFTVGASAAAVIAALVAMLAALAIVIELLARQAVIYAGVL
                     FLPLGFAAMVWPHLLRWAQRLVEVVVVAVVAKFLIISVLVLGAAAYTAAVPGGGPFDS
                     QAPPGSTLLVGLLLVALAALSPAALFWMLPTFEAAALAQFHASARAPASAAAHTVERS
                     VYHVGLRRLWRDRGRQSQSAGRVMVVRPGADVVVRHPRYTPPADGATPTQPARPDIEA
                     RHARAV"
     gene            833..2362
                     /locus_tag="VFV25_10250"
     CDS             833..2362
                     /locus_tag="VFV25_10250"
                     /inference="COORDINATES: protein motif:HMM:NF042935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO6880 family protein"
                     /protein_id="HEY1393748.1"
                     /translation="MPEPSEEARYQYGPLERRGVLLGLSAGQVATLGGAGALALLALH
                     VLPGAAAGLLSSVIAIVGATSALLPVAGRPPEAWLPLLAAYGLRRLLGRRYRSRAHLQ
                     GHLVVVREDGSGLEALPPEEKPRLLRDLRFLEVEAGDEAHPVGAVKDVRRHTYAGVLR
                     VRARTSFNLLEEAGQAAAVQAWASILAGYALASSPISRLQWIERTLPEDGQPLRRHFE
                     ERSAQGAPPAALRLYSEAVAGVRRAGIRHECLLVLQVDARRAWRQVRQVGRSSLDAGA
                     CGLLVRELAALGERLEAAGITVEAVLGPRAIAEVVRTAFEPDARPSLRVVHGDGADAG
                     PHPRNAWPQETAEGFAHHRAGPRAFHATYHVREWPRIQVGPGFLAPLLLGSEGLRTVS
                     ITMEPVPASQAVKELRRALASDASDDTLREKAGWLPSLRRQREQENVLRAERELADGH
                     ASYRFSAYVMVSASGAVELEAACAEVEQAASRSHLELERLLAQQELAFTYTLPLCEGL
                     A"
BASE COUNT          297 a          913 c          924 g          382 t
ORIGIN      
        1 ctcaaggccg ccttcggcta tgcgcccctg gccgtggtgc tgagcgcgat cgcggtgagc
       61 ctgacccaga cgctgctggc agttaccgac agcttcagcg acttcatgct caacggctac
      121 caggcacagg tggcggccac catcggcagc ctggccggcg tgctggcagc cggagcagcc
      181 ggcaccgtct tcacggtggg cgcctcagcg gccgccgtga tcgcggccct ggtggcgatg
      241 ctggccgcgc tcgccatcgt gatcgagctg ctggcccgcc aagcggtcat ctacgcgggc
      301 gtgctcttcc tgccgctggg cttcgcggcc atggtctggc ctcacctttt gcgctgggcg
      361 cagcgccttg tcgaagtggt ggtagtcgcc gtggtggcca agttcctgat catctcggtg
      421 ctggtgctgg gagcggcagc ctacacggcc gcggtgccgg gtggcgggcc cttcgacagc
      481 caggcgccgc cgggcagcac gctgctggtg gggctgctcc tggtcgccct ggcggcgctc
      541 tcgcccgccg cgctgttctg gatgctgccc acattcgagg cggcggcgct ggcccagttc
      601 cacgccagcg cgcgagcgcc tgcgtcggcg gccgcgcaca cggtcgagcg ctcggtctac
      661 cacgtgggcc tgcgtcggct gtggcgcgac cgagggcggc agtcgcagag cgccggccgg
      721 gtcatggtcg tccggccggg cgctgatgtg gtcgttcggc acccgcgcta cacccctcca
      781 gccgacggtg cgacgcccac ccagccggct aggccggaca tcgaggccag gcatgcccga
      841 gccgtctgag gaggcccgct accagtacgg cccgctggag cggcgcggcg ttctgcttgg
      901 gctgagcgcc ggccaggtgg ccacgctggg aggcgctggg gccctggcgc tgctggcgct
      961 ccacgtcctg cccggcgccg cggccgggct tctgtctagt gtgatcgcga tcgtaggcgc
     1021 cacctccgcg ctgctgccgg tcgccggccg gccgccggag gcgtggctgc cgctgctcgc
     1081 cgcctatggc ctgcgccggc tgctgggccg ccgctatcgc tcccgcgccc acctccaggg
     1141 ccacctggtc gtcgtgcgcg aggacggcag cggacttgag gccctgccgc ccgaagagaa
     1201 gccacggttg ttgcgcgacc tgcgcttcct ggaggttgag gcgggcgacg aggcgcatcc
     1261 cgtgggcgcg gtcaaggacg tccgtcgcca cacctacgcg ggcgtccttc gcgtccgcgc
     1321 gcgcacctcc ttcaacctgc tggaggaggc aggccaagcc gcggctgtcc aggcgtgggc
     1381 ctcgatcctg gccggctacg cgctggcctc gtcgccgatc agccggctgc agtggatcga
     1441 gcgcacgctg cccgaggacg gccagccgct gcggcggcac ttcgaggaac gatcggccca
     1501 gggcgcgcca ccggctgcgc tgcgccttta ctccgaggcc gtcgccggcg tgcgacgggc
     1561 aggcatccgc cacgagtgcc ttctggtcct ccaggtcgac gcccgccggg cctggcgtca
     1621 ggtccgccag gttggccgga gcagcctcga tgccggagcc tgcggcctgc tcgtccgcga
     1681 gctggcggcg ctgggcgagc gcctggaggc ggccgggatc acggtcgagg cggtgctcgg
     1741 cccgagggcg attgcggaag tggtgcgcac cgcattcgag ccggacgccc gcccgagcct
     1801 gcgggtagtc cacggcgacg gcgctgatgc cgggccacac cctcgcaacg cctggccgca
     1861 ggaaacggcg gagggcttcg cccaccaccg cgcaggcccg cgtgcgttcc acgccaccta
     1921 ccacgtgcgc gagtggccgc gcatccaggt ggggccgggc ttcctggccc cgctgctgct
     1981 gggctcggaa ggcctgcgca cggtctccat aacgatggag ccagtcccgg cttcgcaggc
     2041 cgtcaaagag ctgcgccggg cgctggccag cgacgcgtcc gacgacaccc tgcgcgagaa
     2101 ggccggctgg ctcccaagct tgcggcgtca gcgtgagcag gagaacgtgc tgcgcgccga
     2161 gcgggagctc gccgatggcc acgctagcta ccgcttcagt gcctatgtca tggtcagcgc
     2221 cagcggcgcg gtcgaactgg aggctgcatg cgcggaggtg gagcaggcgg ccagtcgctc
     2281 acacctggag ctggagcggc tcctggccca gcaggagctg gccttcacct acacgctgcc
     2341 gctctgcgag ggcctggcat gatcgctcgc tcccgccaga tcccgcaccg tgttacgacc
     2401 cgccacctgg gcgccgccta tcccttcatg gccgagggcg ggctgggcga tcgcggggtg
     2461 ctgctgggca gcgatgcgtt cggcggcccc ttcgtctacg acccctggga gcttta
//
LOCUS       DASUCY010000521         3765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_991488, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000521 DASUCY010000000
VERSION     DASUCY010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991488"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VFV25_10255"
     CDS             <1..205
                     /locus_tag="VFV25_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393749.1"
                     /translation="VLAGLARELWADAFRRMVAEATQRVEEDAALSPEAAATRMRFGV
                     YYYHEAVEPPAQDAASPQQEIKP"
     gene            202..1641
                     /locus_tag="VFV25_10260"
     CDS             202..1641
                     /locus_tag="VFV25_10260"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HEY1393750.1"
                     /translation="MKSPRMQRLLQAWRPTLAGAFGALVALLASCGGGVGSGGTGAPV
                     GYASGTVTGFGSVIVDGTHFDDVNAVVDTEVGPGQVVPAESRLGQHVEVAYQTLGVAT
                     AVHVAAQVSGPVSAVAAPNSLTVLGQAIRINADANAGPATVFAGGYAGISSIAVGDAV
                     EVHGLQRQDDSGAGFIHATRIEKLAALPSYLRVTGPVSRLDSSARSFMLGNQRIDYSA
                     ASLLPQGAVLANGQTVLVFGSALQTGNGGQPVLTAQLVRVKQVEGSGTPAYLGGTVSR
                     LDAASASFLLDGTTVHYSGAAVLPTGRSLASGVYVRVQGSFSADGSLAASQVFIRNQT
                     DEAQLELSGTLSGYNAATQSFQVRNVLVNAASATLVGCSAGLGNGLYVQVEGGIVNGA
                     AVATQVSCVSAPPGAVVDSFGVAGSVNIDARTFTLTPTGGAAIAVSWTGLTYFGDGLT
                     PGSLSGQTVEVEGVFSGSVLTATKVTKGG"
     gene            complement(1650..2099)
                     /locus_tag="VFV25_10265"
     CDS             complement(1650..2099)
                     /locus_tag="VFV25_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEY1393751.1"
                     /translation="MKKQAATGTEVAHETASMRLDKWLWAARFYKSRSLAVEAIDKGR
                     VQVNEQPAKRSRELRVGDCVALRQGERGELPRTVMVKALSEVRGPAPVAQRMYEETAQ
                     SIADREAAASARRYAAEPAQAIEQGRPTKRDRRQLADWQRWSVALDE"
     gene            complement(2137..3228)
                     /gene="hemH"
                     /locus_tag="VFV25_10270"
     CDS             complement(2137..3228)
                     /gene="hemH"
                     /locus_tag="VFV25_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEY1393752.1"
                     /translation="MKYRAEPAHEHGRAARTAVMLVNLGTPEAATPSALRRYLAEFLS
                     DPRVVEIPRLVWLPILHGIILRTRPAKSAAKYASIWTPEGSPLKLWTEKQAKLLRGHL
                     GQRGHDVLVSHAMRYGAPSIATQLTALKAEGASRVLVLPLYPQYSGTTTASACDAVAS
                     WVQQTRQVPELRFVNRYHDDPSYIEALAKRISTHWMAHGRPERLVLSFHGVPKRVLAL
                     GDPYHCECHKTARLLAQRLGLNPREVVVSFQSRFGKAAWLQPYTEPTLRQLAKSGVKR
                     VDVACPGFVSDCLETLEEIAHAGRTAFLTAGGSEFHYIECLNDLPEWIAALGQIALRH
                     LSGWPTQSAADAAMLAEQKARAMAMGARA"
     gene            3320..>3765
                     /locus_tag="VFV25_10275"
     CDS             3320..>3765
                     /locus_tag="VFV25_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEY1393753.1"
                     /translation="MTQTIRALTLDLDDTLWPIWPAIERAEAVLHAWLAAHAPATAGR
                     YGIAALRELCDAVGRSRGDWAHDLSALRRESLRLALADAGDDPALAEPAFECFFAERQ
                     KVDLFDDVLPALERLASRYPLLALSNGNADIARIGLARYFRGSLSAR"
BASE COUNT          525 a         1252 c         1358 g          630 t
ORIGIN      
        1 ggtgctggcc ggcttggcgc gcgagctctg ggccgatgcc ttccgccgca tggtggcgga
       61 ggcgacgcaa agggtagagg aggacgcggc gctgagcccc gaggcggcgg ccacgcgcat
      121 gcgttttggg gtgtattact accatgaggc cgtcgagccc cccgctcagg acgctgcgag
      181 cccccagcag gagatcaagc catgaaatcg ccacgcatgc agcgtctgct ccaggcctgg
      241 cgcccgacgc tggccggcgc tttcggcgcg ctggtcgcgt tgctggcgag ctgcggcggc
      301 ggtgtcggct ccggcggcac cggcgcgccg gtgggttatg cctcaggcac cgttaccggc
      361 ttcggcagcg tgatcgtcga cggcacgcat ttcgacgatg tcaacgctgt cgtcgacacc
      421 gaggtcggcc cgggacaagt ggtgccggcc gaatccaggc tgggccagca cgtggaggtg
      481 gcttatcaga cccttggcgt tgccacggcg gtgcatgtgg cggcgcaggt ctctgggccg
      541 gtgagcgccg tcgcggcacc caactcgctg accgtgctgg gccaggcgat caggatcaat
      601 gccgatgcca atgcggggcc ggcgaccgtc ttcgccggcg gctatgcggg catctccagc
      661 atcgccgtgg gcgacgccgt cgaggtgcat gggctgcaga ggcaggacga cagcggcgcc
      721 ggcttcatcc acgccacgcg catcgagaag ctggcggcct tgccgagcta tctgagggtc
      781 acgggtccgg tcagcaggct ggacagcagc gcccgcagct tcatgctcgg caatcagcgc
      841 atcgactaca gcgccgcgag cctgctgccg caaggcgccg tgcttgccaa tggccagacg
      901 gtgctcgtct tcggcagtgc gctgcagacc ggcaacggtg gccagccggt gctgaccgcg
      961 cagctggtgc gggtcaagca agtcgagggc tcgggcactc cggcttacct gggcggcacg
     1021 gtgagcaggc tggacgccgc gtctgcgagc ttcctgctcg acggcaccac ggtgcactac
     1081 agcggcgctg ccgtcctgcc gaccgggcgc agcctggcca gcggcgtcta tgtgcgggtt
     1141 caagggagct tttccgccga cggttcgctc gcggcgagcc aggtcttcat ccgcaaccag
     1201 accgacgagg cccagctcga gctgagcggc acgctgagcg gctacaacgc cgcgacccag
     1261 agcttccagg ttcggaatgt gctggtcaac gcggcgagtg cgacgctcgt cggctgctcg
     1321 gccgggctcg gcaacggcct ctacgtgcag gtggagggcg gcatcgtcaa cggcgccgcg
     1381 gtggcgacgc aggtgagttg cgtgagcgct ccgccgggcg cggtggtcga cagcttcggc
     1441 gtggcgggct cggtcaacat cgacgccagg acgttcacct tgaccccgac cggcggcgcc
     1501 gcgatcgcag tgagctggac cggcctgacc tatttcggcg acgggctcac gcccggcagc
     1561 ctgagcgggc agaccgtgga agtcgagggg gtgttttccg gcagcgtgct gacggcgacg
     1621 aaggtcacca agggcggata agcgcccgtt cactcgtcga gcgcgacgct ccaacgctgc
     1681 cagtcggcaa gctgccgcct gtcgcgcttg gtgggccggc cttgctcgat ggcttgtgcc
     1741 ggttcggcgg cgtagcggcg ggcgcttgcc gcggcctcgc ggtcggcaat gctctgcgcc
     1801 gtttcctcat acatccgctg ggccaccggc gctgggccgc gtacctcgct caatgccttg
     1861 accatcacgg tgcgcggcag ctcgccgcgc tcgccctggc gcagcgcgac gcagtcgccc
     1921 acgcgcaact cgcgcgagcg cttggccggc tgttcgttga cttgcacgcg gcccttgtcg
     1981 atcgcctcga ccgccaggct gcgcgacttg tagaagcgcg cggcccacag ccatttatcc
     2041 agccgcatgc tggcggtctc atgggcgact tcggtgccgg tcgcggcttg cttcttcatc
     2101 aggctgagga ttgtgcgctc gccgccgcag gcctgctcag gcgcgggcgc ccatagccat
     2161 ggcgcgggcc ttctgctccg ccagcatcgc ggcgtcggcc gcggactggg tcggccagcc
     2221 gctcagatgc ctgagcgcaa tctgacccag cgcggcgatc cattccggca ggtcgttgag
     2281 gcattcgatg tagtggaatt cgctgccgcc agcagtcagg aaggctgtgc gtcccgcgtg
     2341 agcgatttcc tccagcgtct ccaggcagtc gctgacgaag ccggggcagg ccacgtccac
     2401 gcgcttgacg ccggacttcg ccaattgcct cagcgtcggc tcggtatagg gctgcagcca
     2461 ggcggccttg ccgaagcggc tctggaagct gaccacgact tcccgggggt tcaggcccaa
     2521 gcgctgcgcc agcaggcgtg ccgtcttgtg gcattcgcag tggtaggggt cgcccagcgc
     2581 cagcacgcgc ttgggcacgc cgtgaaagct cagcaccagg cgctcggggc ggccatgggc
     2641 catccagtgc gtgctgatgc gcttggcgag cgcctcgatg tacgaggggt cgtcgtgata
     2701 gcggttgacg aagcgcagct ccggcacctg tcgcgtttgc tggacccagc ttgccaccgc
     2761 gtcgcaggcg ctggcggtgg tggtgcccga gtattgcggg tagagcggca gcaccagcac
     2821 gcggcttgca ccttcggcct tgagtgcggt cagctgggtg gcgatgctcg gtgcgccgta
     2881 gcgcatcgcg tggctcacca gcacatcgtg tccgcgctgg cccagatggc cgcgcagcag
     2941 cttggcctgc ttctcggtcc atagcttcag cggcgagccc tcgggcgtcc agatgctggc
     3001 gtacttggcc gccgacttgg ccgggcgcgt gcgcaggatg atgccgtgca ggatgggcag
     3061 ccataccagg cgcggaattt cgacgacgcg cggatcgctc aggaactcgg ccagatatcg
     3121 gcgcagggcg ctcggcgtcg cggcttcggg cgtgcccagg ttgacgagca tcaccgcggt
     3181 gcgcgcggcg cggccatgtt cgtgggcggg ttctgctcgg tatttcatgc gttgcgtgct
     3241 gacactgtgg cttcagcgcg ccgcaactgt agcggacgcg caaggggcat gcgggccagg
     3301 caggcacaat ccggcgtcca tgacgcaaac catacgcgct ctcacgctgg acctggacga
     3361 cacgctatgg ccgatctggc ccgccatcga gcgggcggaa gcggtgctgc atgcctggct
     3421 ggccgcgcat gcgcctgcaa cggcagggcg ctatggcatc gccgccttgc gcgagctgtg
     3481 cgatgctgtg gggcgttcac gcggcgactg ggcccatgat ctctcggcct tgcgccgcga
     3541 gagcctgcgc ctggcgctgg ctgatgccgg cgacgacccg gctctggcgg aaccggcctt
     3601 cgaatgcttc ttcgccgagc gccagaaggt cgacttgttc gatgatgtgc tgccggccct
     3661 ggagcgcctg gcgagccgct atccgctgct ggcgctgagc aacggcaacg cagatatcgc
     3721 acgtatcggc ctggcgcgct atttccgcgg cagcctcagc gcgcg
//
LOCUS       DASUCY010000522         3382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1377350, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000522 DASUCY010000000
VERSION     DASUCY010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1377350"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="VFV25_10280"
     CDS             complement(<1..726)
                     /locus_tag="VFV25_10280"
                     /inference="COORDINATES: protein motif:HMM:NF019365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HEY1393754.1"
                     /translation="MLDRRRLIAAAALLPLASRAAADGPHWQDQGDAAASNTAALNAD
                     ARLPLRHESAADYRVTSYRVIEGLAPGSYRLRAKARSSGGQPSVFAFARVAGYSLART
                     HPLASAEARELVIPGIPVEDGTLAIGLHSDALAGQWAELSNIELVRESAPRPFLAGGD
                     VSVLSWMEKAGAQYADRRGRVRDALQILKDSGHSIVRLRLYESPGSGHGVDGWYWPAH
                     SMDLGDVLKLARRAAALGLQIELT"
     gene            complement(719..1699)
                     /gene="araH"
                     /locus_tag="VFV25_10285"
     CDS             complement(719..1699)
                     /gene="araH"
                     /locus_tag="VFV25_10285"
                     /EC_number="7.5.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose ABC transporter permease AraH"
                     /protein_id="HEY1393755.1"
                     /translation="MNHQSTLASVPGTGGARRAAWQDYSMPIGYALLFVVLSLSVQGF
                     FSTVNIVGLLLSVAQIGMVACTMMLCLASRDFDLSVGSTVAFSGVFGAILLERTQSLP
                     LAVGGGLLAGALIGAINGALIAYLRINALIATLATMLMVRGLAFIVSHGQSVGIANDA
                     FIRFGDARLAGVPAPVLLAAACFVVFGVLLNHTVFGRNTLAIGGNPEAARLAGVKVER
                     LRVWIFALQGIVTAMAGLMLASRITSGQPNAAQGFELDVISACVLGGVSLQGGKARIF
                     GVLIGVLIMGTLANVMNLLNVDVFYQYLMRGLILLAAVLLDQMNAKGGRA"
     gene            complement(1696..3207)
                     /gene="araG"
                     /locus_tag="VFV25_10290"
     CDS             complement(1696..3207)
                     /gene="araG"
                     /locus_tag="VFV25_10290"
                     /EC_number="7.5.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015612 - ABC-type L-arabinose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042882 - L-arabinose transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HEY1393756.1"
                     /translation="MAAYLQFDNVSKQFPGVKALDGVSFEAHASQVHGLLGENGAGKS
                     TLLKILGGQYRPDGGRLLLAGRECHFASAGDAIRAGIAVIHQELQYVSDLTVTENMLL
                     GRLPRRFGFVDKRAARALVAQRLQEIGVDLDPDARLSELSIAQRQMVEICRAVLQDAQ
                     VIAFDEPTSSLSHRETEILFRLVKSLRAQGRTLIYISHRLEELYELCDACTIFRDGRK
                     VASHPVMAEVPRDRLISDMVGRELSDIYDYQARPLGAERLRVRALRGGPIRRAQDFSV
                     RAGEVLGIFGLVGAGRSELMRLLYGADKRAEGEVLLDGRQVPAGGPSAAIDAGIVLCP
                     EDRKEQGILAQASVAENINISVRRKELLGGLFLRRRREAERADGFIRRLRIKTPHRRQ
                     EIRLLSGGNQQKVILARWLAEPNLKVLILDEPTRGIDVGAKNEIYKLIHEVAASGCCV
                     IVVSSELPEVLGISDRVIVMRDGAVSGELPREAANEAAVLSLALPDLNPGATA"
     gene            complement(3210..>3382)
                     /locus_tag="VFV25_10295"
     CDS             complement(3210..>3382)
                     /locus_tag="VFV25_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006761975.1"
                     /note="with AraGH is involved in the transport of
                     arabinose into the cell; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY1393757.1"
                     /translation="GFYGTVLISPRRHGYETSLDMYRWITEGVAPPPLTLTTGTVVTR
                     ANLSEVRRKMGL"
BASE COUNT          545 a         1178 c         1168 g          491 t
ORIGIN      
        1 ggtcagttcg atctgcaggc ccagcgccgc ggcccgccgg gccagcttga gcacgtcgcc
       61 cagatccatg ctgtgcgcag gccaatacca gccgtccacg ccatggccgc tgccgggcga
      121 ttcgtagagc cgcagccgca cgatgctgtg gccgctgtcc ttgaggatct gcagcgcgtc
      181 gcggacacgg ccgcgccggt cggcatattg cgcgccggcc ttctccatcc agctcagcac
      241 cgacacatcg ccgccggcca ggaaggggcg cggtgccgat tcgcgcacga gttcgatgtt
      301 ggacagctcg gcccactgcc cggcaagcgc gtccgaatgc aggccgatgg cgagcgtgcc
      361 gtcttccacg gggatgccgg ggatgaccag ctcccgcgcc tcggcgctgg ccagcggatg
      421 ggtgcgggcc aggctgtatc cggctacccg cgcgaaggcg aacaccgagg gctggccgcc
      481 gctgctgcgt gccttggccc gcagacggta ggagcccggc gccaggcctt cgatcacgcg
      541 gtaagaggtg acgcggtagt cggccgcact ctcgtggcgc agcggcagcc gcgcatcggc
      601 gttcaatgcc gcggtgttgg atgcggcggc gtcgccctgg tcctgccagt gcggcccgtc
      661 tgcggcggct cgggacgcga gcggcagcag cgccgcggcg gcgatgagcc gcctgcgatc
      721 aagcacggcc gccctttgca ttcatctggt ccagcagcac cgcggccagc aggatcaggc
      781 cgcgcatcag gtactggtag aacacgtcca cgttcagcaa attcatcaca ttggccaggg
      841 tgcccatgat cagcacgccg atcagcacgc cgaagatgcg cgccttgccg ccttgcagtg
      901 agacgccgcc gagcacgcag gccgagatga cgtccagctc gaagccctgg gcggcattgg
      961 gctggccgct ggtgatgcgc gaggcgagca tcagcccagc catcgcggtg acgatgcctt
     1021 gcagcgcgaa gatccacacg cgcagccgct ccaccttcac gccggccagg cgcgccgctt
     1081 ccgggttgcc gccgatggcc agggtgttgc gcccgaagac cgtatggttg agcagcacgc
     1141 cgaacacgac gaagcaggcc gcggccagca gcaccggggc cggcaccccc gccagccgcg
     1201 cgtcgccgaa gcggatgaag gcatcgttgg caatgcccac cgactggcca tgcgagacga
     1261 tgaaggccag gccgcgcacc atcagcatag tcgccagggt ggcgatcagc gcgttgatgc
     1321 gcaggtaggc gatcagcgcg ccgttgatgg cgccgatcag cgcgccggcc agcaagccgc
     1381 cgcccacggc cagcggcagg ctttgcgtgc gctccagcag gatggcgccg aacacgccgg
     1441 agaaggcgac ggtagagccc accgagagat cgaagtcccg cgaggccagg cacagcatca
     1501 tggtgcaggc caccatgccg atctgcgcaa ccgagagcag caggccgacg atgttgacgg
     1561 tggagaagaa gccttgcacc gacagcgaaa gcacgacgaa gagcagggca tagccgatcg
     1621 gcatgctgta gtcctgccac gcggcgcggc gggcgccgcc ggtcccggga acgctcgcca
     1681 gagtcgattg atggttcatg cagtagctcc tggattcaag tcgggcaggg caaggctcag
     1741 caccgctgcc tcgttggcgg cttcgcgcgg cagctcgccg ctgacggcgc cgtcgcgcat
     1801 cacgatcacg cggtcggaga tgcccagcac ctcgggcaac tcgctggaca cgacaatgac
     1861 gcaacagccg ctggcggcga cctcgtgaat cagcttgtag atctcgttct tggcgccgac
     1921 gtcgatgccg cgcgtgggct cgtcgaggat cagcaccttg aggttcggct cggccagcca
     1981 gcgcgcgagg atgaccttct gctggttgcc gccggacagc aggcggatct cctggcggcg
     2041 atgcggcgtc ttgatgcgca ggcgccggat gaagccgtcg gcccgctcgg cctcgcgccg
     2101 gcggcgcagg aagaggccgc ccagcagctc cttgcggcgc acactgatgt tgatgttctc
     2161 ggcgacggag gcctgggcga ggatgccttg ctccttgcgg tcttccgggc agagcacgat
     2221 gcccgcatct atcgcggcgg acgggccgcc cgcaggcacc tgccggccgt ccagcagcac
     2281 ctcgccctcg gcgcgcttgt ccgcgccgta gagcaggcgc atcagctcgc tgcgccccgc
     2341 gccgaccagc ccgaagatgc ccagcacctc gcccgcgcgc acggaaaagt cctgcgcacg
     2401 ccgtatcggc ccgccgcgca gggcccgcac gcgcaggcgc tcggcgccca gggggcgggc
     2461 ctggtagtcg tagatgtcgc tgagctcgcg ccccaccatg tcggagatga gccggtcgcg
     2521 gggcacctcg gccatcaccg gatgcgaagc gaccttgcgg ccgtcccgga agatggtgca
     2581 ggcgtcgcac agctcgtaga gctcttccag ccggtgcgag atgtagatca gcgtgcggcc
     2641 ttgcgcgcgc aggctcttca ccaggcggaa gaggatctcc gtctcgcggt gcgacaggct
     2701 gctcgtgggc tcgtcgaagg cgatgacctg cgcatcctgc aggacggccc ggcagatctc
     2761 caccatctgg cgctgggcga tcgagagttc ggagaggcgc gcatcgggat ccaggtcaac
     2821 gccgatttcc tgcaggcgct gcgccaccag ggcgcgcgcc gcgcgcttgt cgacgaagcc
     2881 gaagcgccgc ggcagccgcc ccagcagcat gttctcggtc acggtcaggt cgctgacgta
     2941 ctgcagctcc tggtgaatga ccgcgatgcc ggcgcggatc gcgtcgccgg cgctcgcgaa
     3001 atggcattcg cgccccgcca gcagcagccg cccgccgtcg ggccggtact gcccgcccag
     3061 gatcttgagc agcgtggact tgcccgcgcc gttctctccg agcagaccgt gcacctggct
     3121 cgcatgcgcc tcgaagctca cgccgtccag cgccttcacg ccggggaatt gcttgctgac
     3181 gttgtcgaat tgcaaatagg ccgccatgct cacaacccca tctttctgcg cacttcgctc
     3241 aggttggcgc gcgtgaccac cgtgccagtg gtcagcgtca agggcggcgg cgcgacgccc
     3301 tcggtgatcc accggtacat gtccagcgag gtctcgtagc catggcgccg cgggctgatc
     3361 agcacggtgc cgtagaagcc tg
//
LOCUS       DASUCY010000523         2924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1569999, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000523 DASUCY010000000
VERSION     DASUCY010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1569999"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(361..1839)
                     /locus_tag="VFV25_10300"
     CDS             complement(361..1839)
                     /locus_tag="VFV25_10300"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEY1393758.1"
                     /translation="MDMKTSPLASLKDASLLKTDSLINGEWIKGTRRFGVFDPATGLK
                     LADVARLGAAETQAAIKAASAAWPAWAKKTAKERGGVLMKWFHLLHQHADDLARIMTA
                     EQGKPLPEAKGEVHYGASFIEWFAEEGKRVYGETIPTTDNNKRYLVLKQPIGVCAAIT
                     PWNFPIAMITRKVAPALAAGCPVVIKPAEATPLSALAVAELAQRAGMPAGVLNIVTSD
                     AEGSIEVGKVLCASDTVQHLSFTGSTEVGRILMQQCAPTIKKLSLELGGNAPFIVFND
                     ADIDSAVDGALASKYRNAGQTCVCANRLYVQEGIYETFVTKLAEKTKGIKVGNGFEAG
                     INQGPLIDDQAIAKVERHVADALAKGARLVSGGKKIGAHFYEPTVLADATPEMLCSRE
                     ETFGPVAPVFKFKTEAEAIALANDTEFGLASYFYSRDIGRIFRVGEALEYGMVGVNTG
                     LIATAEVPFGGVKQSGLGREGAQQGMDDYVEIKYLCLGDILK"
     gene            complement(1915..2868)
                     /gene="speB"
                     /locus_tag="VFV25_10305"
     CDS             complement(1915..2868)
                     /gene="speB"
                     /locus_tag="VFV25_10305"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014900442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEY1393759.1"
                     /translation="MTREPNQPLSGNAMPRFAGLATMMRLPAATSAQGLDAAFIGVPL
                     DTGTSNRAGARFGPRQIRAESALLRPYNMATGAAPFDTLQVADLGDVPINTYSLEKSL
                     PIITAFYDDVLAHGCKPLTLGGDHTIALPILRAIAGKHGPVALVHVDAHADINDEMFG
                     ERIAHGTPFRRAVEEGLLACDKVWQIGLRGTGYAADDFDWPRAQGFTVVQAHEVWYQS
                     LAPLMAQVRERIGPRHPVYITFDIDGIDPSFAGGTGTPEIGGLSVPQALEIVRGCRGL
                     NVVGADLVEVAPPYDPSGNTALLGANLLYEMLCVLPSVAHR"
BASE COUNT          463 a          916 c         1002 g          543 t
ORIGIN      
        1 gtcaagcaaa gccttccgtg cgcatgcagg gcatagtgag agttcgggtc gttgtgcgtg
       61 gccggagctc gtaacaagca gtcgacggac tccaactcgg agtcaggtcc gttgagctag
      121 cgcaggggct cgtagaaggt cgacgagctg acgcgttccg tacgaggcat gcatgaccgc
      181 ggcagggtac tcgctcacat gtttgagcag agattggcgc gccatctgcg tcaggggatg
      241 ccctggcggg cgaccaccca catcggctgg cggatcgcgg cacgcgaccg gatgccggga
      301 gtgacctgcg agttgaagac gccgccatct ctggcgccat ccctggcgct atttctggcg
      361 ctatttcagg atatcgccca ggcacaaata cttgatctcg acatagtcgt ccatgccttg
      421 ctgcgcccct tcgcggccca ggccggattg tttgacgccg ccaaagggca cttcggccgt
      481 cgcgatcagg cccgtgttga cgcccaccat gccgtactcc agcgcctcgc cgacgcggaa
      541 gatgcggccg atgtcgcggc tgtagaagta gctggccaag ccgaactcgg tgtcgttcgc
      601 gagcgcgatg gcttctgcct cggtcttgaa cttgaacacc ggcgccaccg ggccgaaggt
      661 ctcttcgcgc gagcacagca tctcgggcgt ggcgtcggcc agcacggtgg gctcgtagaa
      721 gtgcgcgccg attttcttgc cgccgctcac gagcctcgcg cccttggcca gggcatcggc
      781 cacatggcgc tccaccttgg cgatggcctg gtcgtcgatc agcgggcctt gattgatccc
      841 agcctcgaaa ccgttgccga ccttgatgcc tttggtcttt tccgccagct tggtgacgaa
      901 ggtctcgtag atgccctcct gcacatacaa gcgattggcg cagacgcaag tctggccggc
      961 attgcggtac ttgctcgcca gggcgccgtc caccgcgctg tcgatgtcgg catcgttgaa
     1021 gacgatgaaa ggcgcattgc cgccgagttc gagcgagagc ttcttgatcg ttggcgcgca
     1081 ttgctgcatc aggatgcgcc cgacttcggt ggagccggtg aaggacagat gctgcaccgt
     1141 gtcgctggcg cacagcacct tgccgacttc gatggagccc tcggcgtccg aggtgacgat
     1201 gttaagcacg ccggccggca tgccggcgcg ctgcgccagc tcggccactg ccagtgccga
     1261 cagcggcgtg gcttcagccg gcttgatcac cacggggcag ccagcggcca gcgccggggc
     1321 caccttgcgg gtgatcatcg cgatcggaaa attccagggc gtgatggccg cgcagacacc
     1381 gatgggctgc ttgaggacga ggtagcgctt gttgttgtcg gtggtcggga tcgtctcgcc
     1441 atagacgcgc ttgccttctt cggcgaacca ttcgatgaag ctcgccccat agtggacttc
     1501 gcccttggcc tcgggcagcg gcttgccttg ctcggcagtc atgatgcgtg ccagatcgtc
     1561 cgcatgctga tgcaggaggt gaaaccactt catcagcacg cccccgcgct ccttggcggt
     1621 cttcttggcc caggcgggcc aggcggcact ggcggccttg atggcggctt gggtctccgc
     1681 ggcgccgagc ctggccacgt ctgccagctt caggccggtg gccgggtcga agacgccaaa
     1741 gcgacgggtg cccttgatcc attcgccgtt gatgagcgaa tcggtcttca ggaggctggc
     1801 gtccttgagg ctggccagcg gcgaggtctt catgtccatg tcgtgtcttt cgtgaaggtc
     1861 gcgatgatgc atgaatctac cgcttggcgc gcaagcgttc ggcagctcgg gcattcatcg
     1921 atgtgcaacg ctgggcagga cgcacagcat ttcgtacagc aggttggcgc ccagcagcgc
     1981 ggtattgccc gaagggtcat agggcggcgc cacctcgacc aggtctgcgc ccaccacgtt
     2041 gaggccgcgg cagccgcgca cgatctccag ggcctgcggc acgctgaggc cgccgatctc
     2101 gggcgtgccg gtgccgccgg cgaacgaggg gtcgatgccg tcgatatcga aagtgatgta
     2161 gaccgggtgc ctggggccga tgcgttcgcg cacctgggcc atcagcggcg cgagcgactg
     2221 gtaccaaacc tcgtgcgcct gcaccacggt gaagccttga gcgcgcggcc agtcgaagtc
     2281 gtcagccgcg tagccagtgc cgcgcaagcc gatctgccag accttgtcgc aagccagcag
     2341 gccctcttcg accgcgcggc gaaacggcgt gccgtgcgcg atgcgctcgc cgaacatttc
     2401 gtcgttgatg tccgcatgcg cgtccacatg caccagcgcc accggaccgt gcttgccagc
     2461 gatcgcgcgc aggatcggca gcgcgatggt gtggtcgccg cccagtgtga gcggcttgca
     2521 gccgtgcgcc agcacatcgt cgtagaacgc ggtgatgatg ggcagcgatt tttcgagcga
     2581 gtaggtgttg attggcacgt cgcccaggtc agcgacctgc agggtgtcga agggtgcggc
     2641 tccggtggcc atgttgtagg ggcgcagcag ggctgattcg gcacggatct gtcgcggccc
     2701 gaagcgcgcg ccggcgcggt tggaggtgcc ggtatccagc ggcaccccga tgaaagcggc
     2761 atccaggccc tgcgcgctgg tggcggcggg cagccgcatc atggtggcca ggcctgcgaa
     2821 gcggggcatg gcattgccgc tcagaggctg gttcggttca cgtgtcatgt tgcaagctcg
     2881 aagagggcct gggcgtgccc ctcgcttcct cgcgggggaa ggcg
//
LOCUS       DASUCY010000524         6371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_783058, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000524 DASUCY010000000
VERSION     DASUCY010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 6371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6371
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783058"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1504
                     /locus_tag="VFV25_10310"
     CDS             <1..1504
                     /locus_tag="VFV25_10310"
                     /inference="COORDINATES: protein motif:HMM:NF024905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEY1393760.1"
                     /translation="ETSLSPRDERLAAMLYGVLEARDQLLACELDLDLVHADAMGTAV
                     LPHLQAVMDDTAAALDALADALLLGRQPEASGLDATERLSAALASIDAPDTPAAEATA
                     ATATATGQTRTLLRNIVRRVGRVVTEVAQLHELARGERRADLSAQAQHWGLYVTPTAW
                     PLQALRNQLNGRSPVLRYAVRLALALGVGFVVALHLPWASHKHWILLTIAVVMRINLA
                     QTLERRNARVGGTLLGCLVAAGLLALHPSVAMIYMVIAMSTAVAHAFVLPRYFVAAVA
                     ATITGLMQSHLLIAGNFVITERLGDTVLGALLAWGCSYVLPAWERRQLPGLVNRVLRA
                     QAKIASQALAMHEQQNSDTPWRLARREAYDSLSALTLAAQRTLAEPRAVQPPLQPLET
                     LQLRSYRMLTHLSSMKAMLTQRRGELDLERVKPALKAAAEDIEDALRPGSPAAAQRSG
                     TAEDKLRELVGDAAPPPSTTELTPWLLRRLHLTRLEAHELAQDAASLQGR"
     gene            1592..4165
                     /gene="mutS"
                     /locus_tag="VFV25_10315"
     CDS             1592..4165
                     /gene="mutS"
                     /locus_tag="VFV25_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEY1393761.1"
                     /translation="MSDFSQHTPMMQQYLRIKADFPDTLVFYRMGDFYEVFYDDARKA
                     NRLLDITLTQRGQSGGEPVVMAGVPVHALESYLAKLIKLGEAVAIAEQVGDVATAKGP
                     VERKVLRVVTPGTVTDSELLAERADTLLLAVAQQRQNYGLAWLALSSGQLGLTECGER
                     ELAGWLARLDPAEILVADRDSLPAALRQGHAALTQRPAWQFDSALGLRKLCEQLRVAS
                     LAGFNAQDLNAAHAAAAALLSFAEHTQGRALAHVHTLRVARASELLELPPATHRNLEL
                     TQTLRGEDAPTLLSLLDTCRSGMGSRALRHWLAHPARERGTAIARHGAIATLIDSGCE
                     PLREMLRHISDVERITARVALRQVRPRELAGLRATLQTLPALRATVPDGDELLAGLQA
                     DLQPPPEIEQRLSAAIAAEPAVLLRDGGVIAPGFDAELDELRAISQNCDAFLLDLEAR
                     ERTRTGIANLRVQYNKVHGFYIEVTTSGLDRVPMDYQRRQTLKNAERYITPELKAFED
                     KALSAQERSLAREKLLYEQLLDALQAHLPALTALARALASLDALATLAERATTLSWCR
                     PEFVREPCIEIEQGRHPVVEARLAENGGGSFMPNDCRLDAKRRMLVITGPNMGGKSTF
                     MRQVALIALLAAMGSYVPATSCRLGPIDAIHTRIGAADDLANAQSTFMLEMTEAAAII
                     HGASEHALVLMDEIGRGTSTFDGLALASAIASHLHDKNRAFTLFATHYFELTEFPAKH
                     ERAINVHVSAAESGDDIVFLHEIQPGPASRSYGVQVARLAGMPGGLIRQARATLEALE
                     QQQRNADAQIDLFAAPAPAPEAPEPSPVEAALAALQPDTLSPREALDALYRLKSLQGS
                     T"
     gene            4167..4997
                     /locus_tag="VFV25_10320"
     CDS             4167..4997
                     /locus_tag="VFV25_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome-type protease"
                     /protein_id="HEY1393762.1"
                     /translation="MTYCVAMRLNAGLVFLSDTRTNAGLDQISSFRKMTIYEKPGERV
                     MVMLAAGNLAITQAVRQELQTESIESGGETISIWNCKSMFEAARIVGSAVRKVFTRDA
                     AVLKEHGIEFNINLIFGGQIKGETMRLFNIYAAGNFVEAGIDGVCYFQIGESKYGKPV
                     IDRVVTPETPLDEAAKCALVSMDSTLKSNLSVGLPLDLLVYEAGTLKVDRLVNIDENN
                     AYFRMIRSSWGKRLREVFDSLPAPTWHGEASEYPLTTPAAAQQPQAMNPLTAVRAPDA
                     "
     gene            5197..>6371
                     /locus_tag="VFV25_10325"
     CDS             5197..>6371
                     /locus_tag="VFV25_10325"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEY1393763.1"
                     /translation="MTKFVFVTGGVVSSLGKGIASASLAAILESRGLRVTLIKLDPYL
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFINTRMKRTNNFTTGQIYKSVLEKE
                     RRGDYLGKTVQVIPHVTNEIQEFVKRGAGVGTPDAVDVAIVEIGGTVGDIESLPFLEA
                     VRQMSLKLGPNSTAFVHLTYVPYIAAAGELKTKPTQHTVQKMREIGIQPDALLCRADR
                     RIPAEEREKISLFTNVPEWGVISVWDADTIYKVPRILHEQGLDGLICDRLRLSTPPAN
                     LSRWDALVHEVEHPQAEARIAMVGKYTDLSDSYKSLNEALRHAGIKNHARVKIEYVDS
                     ETITPDNVSQLAKFDAILVPGGFGKRGIEGKITAARYAREQRIPYMGICLGMQVATI"
BASE COUNT         1058 a         2269 c         2118 g          926 t
ORIGIN      
        1 ggaaacctct ctgtcgccgc gcgacgagcg cctggccgcg atgctctacg gtgtgctcga
       61 ggcgcgcgac cagctgctgg cctgcgagct cgacctcgac ctggtgcacg ccgatgcgat
      121 gggcaccgcc gtgctgccgc acctgcaggc ggtgatggac gacacggccg ccgccctgga
      181 tgcgctggcc gacgcactgc tgctgggccg ccaacccgag gcgagcgggc tcgacgcgac
      241 ggagcgactc agtgccgcgc tggcgagcat cgatgcgccg gacacgcctg ccgccgaggc
      301 gacagccgca acagccaccg ccaccggcca gacccgcacg ctgctgcgca acatcgtgcg
      361 gcgcgtcggc cgcgtcgtca ccgaggtggc gcagctgcac gagctggcgc gcggcgagcg
      421 ccgcgccgac ctgagcgccc aggcccagca ttggggcctg tacgtgaccc cgaccgcctg
      481 gccgctgcag gccctgcgca accagttgaa cggccggtcg ccggtgctgc gctatgcggt
      541 gcgcctcgcg ctggcgctgg gcgttggctt cgtcgtcgcg ctgcacctgc cctgggcctc
      601 gcacaagcac tggatcctgc tcaccatcgc cgtcgtgatg cgcatcaacc tcgcgcagac
      661 actggagcgg cgcaatgcgc gggtgggcgg cacgctgctg ggctgcctcg tcgcggccgg
      721 cctgctggcg ctgcatccga gcgtggcgat gatctacatg gtgatcgcga tgagcaccgc
      781 ggtggcccat gctttcgtgc tgccgcgcta tttcgtggcg gcggtggctg cgaccatcac
      841 cggcctgatg cagagccacc tgctgatcgc cggcaacttc gtcattaccg aacgcctggg
      901 ggacaccgtg ctcggcgccc tgctcgcctg gggctgcagc tatgtgctgc cggcctggga
      961 acgccgccag ctgcccggcc tcgtgaaccg cgtgctgcgc gcccaggcca agatcgcgag
     1021 ccaggcgctg gcgatgcacg agcagcagaa ctccgacacg ccctggcgcc tggcccgccg
     1081 cgaggcctac gacagcctct cggccctcac gctggccgcc cagcgcacgc tggccgagcc
     1141 gcgcgccgtg cagccgccgc tgcagccgct ggaaaccctg cagctacgca gctaccgcat
     1201 gctgacccac ctgagctcca tgaaggcgat gctcacgcag cggcgcggcg agctggacct
     1261 cgagcgcgtc aagcccgcgc tcaaggccgc cgcggaagac atcgaagatg cgctgcggcc
     1321 cggcagcccg gccgcagcgc agcgctcggg cacggcggag gacaagctgc gcgaactggt
     1381 cggcgacgcg gcaccgccgc cctcgacgac cgagctgacg ccctggctgc tgaggcgttt
     1441 gcacctgacg cggctggaag cgcatgagct ggcgcaggat gccgcaagtt tgcagggccg
     1501 ctgaggcgta gcacgcgttg cttcgctatt tgtagtggct tttcatatag aaacacgagc
     1561 ggccgccgag aaactatcca attccgggct catgagcgac ttctcacagc acacgccgat
     1621 gatgcagcag tacctgcgca tcaaggccga cttccccgac acgctcgtct tctatcgcat
     1681 gggggacttc tacgaagtct tctacgacga cgcgcgcaag gccaaccgcc tgctcgacat
     1741 cacgctcacg cagcgcggcc agtcgggcgg cgagcctgtc gtgatggccg gcgtgccggt
     1801 gcatgcgctg gagagctatc tcgccaagct gatcaagctg ggcgaggcgg tggcgatcgc
     1861 cgagcaggtg ggcgacgtgg ccacggccaa ggggccggtg gaacgcaagg tgctgcgcgt
     1921 cgtcacgccg ggcaccgtga ccgacagcga actgctggcc gagcgcgccg acacgctgct
     1981 gctggccgtg gcacagcagc ggcagaacta cggcctggcc tggctggcgc tcagcagcgg
     2041 gcagctgggc ctcaccgaat gcggcgagcg cgagctggcc ggctggctgg cgcggctcga
     2101 tccggccgag atcctggtgg ccgaccgcga cagcctgccc gccgctctgc gccaaggcca
     2161 cgcggcgctc acgcagcggc cggcctggca gttcgacagc gccctcggcc tgcgcaagct
     2221 gtgcgagcag ctgcgcgtgg cgagcctggc cggcttcaac gcgcaggacc tgaatgctgc
     2281 gcatgctgca gccgccgcgc tgctgagctt cgccgagcac acgcagggcc gtgcgctggc
     2341 ccatgtgcac acgctgaggg tggcacgcgc cagcgagctg ctggagctgc caccggccac
     2401 gcaccgcaac ctggagctga cgcagacgct gcgcggcgag gacgcgccga cgctgctgtc
     2461 gctgctcgac acctgccgca gcggcatggg cagccgcgca ctgcggcact ggctggccca
     2521 cccggcgcgc gagcgcggca cggccatcgc gcggcatggc gccatcgcga cgctgatcga
     2581 cagcggctgc gagcctttgc gcgagatgct gcgccacatc agcgacgtgg agcgcatcac
     2641 ggcccgcgtg gccttgcgcc aggtgcgccc gcgcgagctg gccggcctgc gcgccacgct
     2701 gcagacgctg ccggccctgc gcgcaacggt ccccgacggt gacgaactgc tcgcgggcct
     2761 gcaggccgac ctgcagccgc cgcccgaaat cgagcagcgc ttgagcgcgg ccatcgcggc
     2821 cgagccggcc gtgctgctgc gcgacggcgg cgtgatcgcg cccggcttcg atgccgagct
     2881 cgacgagctg cgcgcgatca gccagaactg cgacgccttt ttgctcgatc tggaagcgcg
     2941 cgagcgcacc cgcaccggca ttgccaatct gcgcgtgcag tacaacaagg tgcacggctt
     3001 ctacatcgag gtgaccacca gcggcctcga ccgcgtgccg atggactacc agcgccgcca
     3061 gacgctgaag aacgccgagc gctacatcac gcccgagctc aaggccttcg aggacaaggc
     3121 gctgtccgcg caggagcgct cgctggcgcg cgagaagctg ctctacgagc agctgctgga
     3181 cgcgctgcag gcgcatctgc cggcactcac cgcgctggcc cgcgcgctcg cctcgctgga
     3241 tgcgctggcg accctggccg agcgcgccac gacgctgagc tggtgccggc ccgaattcgt
     3301 gcgcgagccc tgcatcgaga tcgagcaggg ccgccatccg gtggtcgagg cgcgcctggc
     3361 ggaaaacggc ggcggctcct tcatgcccaa cgactgccgg ctcgatgcca agcgccgcat
     3421 gctggtcatc actgggccca acatgggcgg caagtcgacc ttcatgcgcc aggtggcgct
     3481 gatcgcgctg ctggccgcga tgggctccta tgtgccggcc acgagctgcc gcctggggcc
     3541 catcgatgcg atccacacgc gcatcggcgc ggccgacgac ctggccaatg cgcaatcgac
     3601 cttcatgctg gagatgaccg aagccgccgc catcatccat ggcgcaagcg agcatgcgct
     3661 ggtgctgatg gacgagatcg gccgcggcac ctcgaccttc gatggcctgg cgctggccag
     3721 cgccatcgcc agccatctgc acgacaagaa ccgcgccttc acgctgttcg cgacgcatta
     3781 tttcgagctc accgagttcc cggccaagca cgagcgggcg atcaacgtgc atgtctcggc
     3841 ggccgagtcg ggcgacgaca tcgtcttcct gcacgagatc cagcccggcc cggcgagccg
     3901 cagctacggc gtgcaggtgg cgcgactggc cggcatgccg ggcggcctga tccgccaggc
     3961 gcgcgcgacg ctggaggcgc tggaacagca gcagcgcaat gccgatgcgc agatcgacct
     4021 gttcgcagct cccgcgcccg cccccgaggc gcccgagccc tcgccggtcg aggcggcact
     4081 cgctgcgctg caacctgata ctctttctcc tcgcgaggcg ctggacgcgc tctatcgcct
     4141 gaaatctctc caaggaagta cctgagatga cttactgtgt cgcgatgcgt ctgaacgccg
     4201 gcctggtgtt cctgtcggac acgcgcacca atgccggcct cgaccagatc agcagcttcc
     4261 gcaagatgac gatctacgag aagccgggcg agcgcgtgat ggtgatgctg gccgccggca
     4321 atctggcgat cacccaggcc gtgcgccagg agctgcagac cgaatccatc gagtccggcg
     4381 gcgagaccat cagcatctgg aactgcaaga gcatgttcga ggctgcccgc atcgtcggca
     4441 gcgcggtgcg caaggtgttc acccgcgatg ccgcggtact gaaggagcat ggcatcgagt
     4501 tcaacatcaa cctgatcttc ggcggccaga tcaagggcga gacgatgcgt ttgttcaata
     4561 tctatgcggc cggcaatttc gtcgaggccg gcatcgacgg ggtttgctat ttccagatcg
     4621 gcgaatcgaa atacggcaag ccggtgatcg accgcgtggt gacgcctgag accccgctgg
     4681 acgaagccgc caagtgcgcg ctggtatcga tggactccac gctcaagtcc aatctctcgg
     4741 tggggctgcc gctggacctg ctcgtctacg aggccggcac gctcaaggtg gaccgtctcg
     4801 tcaacatcga cgagaacaac gcctatttcc gcatgatccg cagctcctgg ggcaagcgcc
     4861 tgcgcgaggt cttcgactcg ctgcccgccc ccacctggca cggcgaggcg agcgaatacc
     4921 cgctcaccac gccggcagcc gcacagcagc cgcaggcgat gaacccgctg acggcggtgc
     4981 gcgcgcccga cgcctgagca gcgcccgcgc tggtctcttt cgtgccgtcc aagccgacgc
     5041 aggtcgactt ggagccgcgg cactcacccc ccaagcaagg cccgctcccc tcgggggagt
     5101 atccggcgta cccgctggac gaggggtcgt catcccaccg tcgatataat gccgctttac
     5161 caccacagcg tttgcgatga gcgggcgctg caagacatga ccaagttcgt ctttgtcacc
     5221 ggcggtgtgg tgtcctccct cggcaagggc attgcgtcgg catctctcgc cgcgatcctc
     5281 gaatcgcgcg gcctcagagt caccctcatc aagctagacc cctacctcaa cgtcgatccc
     5341 ggcacgatgt cgcccttcca gcatggcgaa gtcttcgtga ccgacgacgg ggccgagacc
     5401 gacctggacc tgggccacta cgagcgcttc atcaacacgc gcatgaagcg gaccaacaac
     5461 ttcaccaccg gccagatcta caagagcgtg ctcgagaagg agcgccgcgg cgactatctc
     5521 ggcaagacgg tgcaggtgat cccccacgtc accaacgaga ttcaggaatt cgtcaagcgc
     5581 ggcgccggcg tcggcacgcc tgacgcggtg gacgtggcga tcgtcgagat cggcggcacg
     5641 gtgggcgaca tcgagtcgct gcccttcctc gaggcggtgc gccagatgag cctgaagctc
     5701 gggcccaaca gtacggcctt cgtgcacctc acctacgtgc cctatattgc cgccgccggc
     5761 gagctcaaga ccaagccgac ccagcacacg gtgcagaaga tgcgcgagat cggcatccag
     5821 ccagacgcgc tgctgtgccg cgccgaccgc cgcatcccgg ccgaggagcg cgagaagatc
     5881 tcgctcttca ccaacgtgcc cgagtggggc gtgatctcgg tgtgggacgc cgacaccatc
     5941 tacaaggtgc cgcgcatact gcacgagcag ggcctggacg gcctgatctg cgaccggctg
     6001 cgcctgtcca cgccgccggc caacctgtcg cgctgggacg cgctggtgca cgaggtcgag
     6061 catccgcagg ccgaggcccg catcgcgatg gtcggcaagt acaccgacct gtcggactcc
     6121 tacaagtcgc tcaacgaggc gctgcgccac gccggcatca agaaccacgc gcgggtcaag
     6181 atcgagtacg tcgattccga gaccatcacg ccggacaacg tcagccagct cgcgaagttc
     6241 gacgccatcc tggtgccggg cggcttcggc aagcgcggca tcgagggcaa gatcaccgcg
     6301 gcgcgctacg cccgcgagca gcgcatcccc tacatgggca tctgcctggg catgcaggtc
     6361 gcgaccatcg a
//
LOCUS       DASUCY010000525         4665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_172679, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000525 DASUCY010000000
VERSION     DASUCY010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172679"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VFV25_10330"
     CDS             complement(<1..393)
                     /locus_tag="VFV25_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEY1393764.1"
                     /translation="MTSAAFDLVLRNAQVATASDTFHSDIGIKDGRIVQLGLGLAPGQ
                     REIDAAGRVVTPGGVDAHCHLDQPMEPPTRMADDFASGTRSAACGGTTTVIPFAAQAK
                     GQSLRAAVEDYHHRAEGRAHVDYAFHLIV"
     gene            complement(390..1118)
                     /locus_tag="VFV25_10335"
     CDS             complement(390..1118)
                     /locus_tag="VFV25_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEY1393765.1"
                     /translation="MAQRKLIGVLTPSSNTALEPLTSAMVSGLPGVSAHFARFTVTEI
                     SLREASLRQFDDSKILEAARLLADAHVGVICWSGTSASWLGFERDQQLCARITEATGI
                     PATTSVLALNEALAERGARTLGLVSPYMDEVQQRIVANYADIGIACVAERHLGISINF
                     AFSEVEPETLGGLLREVAAQRPDAMTTLCTNLRAAQLVDGLERELGIPIYDSVSTVVW
                     KALKTIGVDPREVKGWGRLFTESA"
     gene            complement(1138..2445)
                     /locus_tag="VFV25_10340"
     CDS             complement(1138..2445)
                     /locus_tag="VFV25_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1393766.1"
                     /translation="MQANAATLVSSSAIQPSRVRWKIFLMMLFLISINYIDRASLSVA
                     MPLIAKEFDIGPATQGLLLSAFFWTYAFMQIPGGMLADRFGPRVVIAGATLGWGFFQA
                     VAATCTGWVSLLVTRLGLGASEAPIYPAGGKLNGIWMTQNERGRGATLLDGGAPLGAA
                     LGALIIAGLISEFGSWRTSFVLAGLGTMVAGLFAWWYIRNHPRQHPAVNDAEAAYIEA
                     AHAADHAAEPANVSGKVLDFFKYRSVWGMFFGWMCFNALFYGLLTWMPTYLSKVHGLD
                     IKQMGGAVFIMFFSGFVGEMVGGWLADKWKAAGASQAKVLRTLFGIASVIATIAIYSV
                     ARFKDPVTVVILLSTTLFFLRWCGLFWCIPSILGTRNRVGFLGGTMNLGGNCAGIGVP
                     IVVGLIVQATGSYDMALTLFAVAGAGLFVCSTLLIDYSKKLPV"
     gene            complement(2551..2802)
                     /locus_tag="VFV25_10345"
     CDS             complement(2551..2802)
                     /locus_tag="VFV25_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393767.1"
                     /translation="MRLAESAQQRHEDAIAGIAVERILRAGIVEQRMAGGAHQHGRAL
                     ADIGRDQLEAAGLLTGLRKSNPAPLRHPILKKTSCQHHC"
     gene            2816..3478
                     /locus_tag="VFV25_10350"
     CDS             2816..3478
                     /locus_tag="VFV25_10350"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY1393768.1"
                     /translation="MLSHCDTDHVGGAAAVMKALRVDSQWSSLEPGHALLGQGIPATR
                     CEAGQGWEWDGVRFELLQPAPALYERPGVKPNALSCVLRVSGGGRSALLTGDIERDGE
                     AAMVAAYGAALSSDLLIVPHHGSKTSSSAAFLDAVQPRIAVMQAGYRNRYGHPSPAVL
                     ASYRERGIHVMLSASCGAWRWRPGVQSCQRDIARRYWQHVWHHPDLPQYEGSGLELAD
                     PD"
     gene            3507..>4665
                     /locus_tag="VFV25_10355"
     CDS             3507..>4665
                     /locus_tag="VFV25_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013342622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEY1393769.1"
                     /translation="MSIHVALNHVTHYRYDRRVGLSPQIVRLRPAPHCRTPILSYSLR
                     IEPVQHFINWQQDPFANYLARLVFPEETTEFKITVDLVAEMAVYNPFDFFLEPGAEHY
                     PFDYEPGLAHDLAPYLAKGPLTPRFKALLDSISTDKVRTIDFLVSLNQRLQQDIRYLI
                     RMEPGVQTPEETLERAAGSCRDSGWLLVQALRHLGLAARFVSGYLIQLKPDVKALDGP
                     SGAEADFTDLHAWCEVYLPGAGWIGLDPTSGLLAGEGHIPVACTPEPSSAAPVTGEVD
                     ESEVEFGHTMQVTRVFESPRVTKPYTEEQWQQVLALGHEVDRGLDKMDVRLTMGGEPT
                     FVSVDDRDGDEWNTTALGPTKRGYATELVQKLRAKYGAGGFLHFGQGKWYPG"
BASE COUNT          799 a         1545 c         1591 g          730 t
ORIGIN      
        1 cacgatcagg tggaaggcgt agtccacatg cgcgcgacct tcggcgcggt ggtggtagtc
       61 ctccaccgcg gcgcgcagcg actggccttt ggcctgggct gcgaagggga tcacggtggt
      121 ggtgccgccg catgccgcag aacgtgtgcc gctggcgaag tcgtcggcca tgcgcgtggg
      181 cggctccatc ggctggtcga gatggcagtg cgcatccacg ccaccgggcg tgaccacgcg
      241 gcccgcggcg tcgatctctc gctgcccggg cgcaaggccg aggccgagct gcacgatgcg
      301 gccgtccttg atgccgatgt cggaatggaa ggtgtcgctg gcggtggcta cctgcgcgtt
      361 gcgcagcacc aggtcgaagg cggcggaagt catgcggatt cggtgaagag tcggccccac
      421 cccttgacct cgcgcggatc gacgccgatg gtcttgagcg ccttccacac caccgtggag
      481 accgagtcgt agatcgggat gcccagctca cgctccagcc cgtccaccag ctgcgcggcg
      541 cgcaggttgg tgcacagcgt ggtcatcgcg tcggggcgct gggcggccac ttcgcgcagc
      601 aggccgccca gtgtctcagg ctcgacctcc gagaaggcaa agttgatcga gatgcccaga
      661 tggcgctcgg ccacgcaggc tatgccgatg tcggcatagt tggcgacgat gcgctgctgc
      721 acctcgtcca tgtagggcga gaccaggccc agcgtgcgcg cgccgcgctc ggccagtgcc
      781 tcgttgagcg ccagtaccga ggtggtggca gggatgccgg tggcctcggt aatgcgtgcg
      841 cagagctgct ggtcgcgctc gaagccgagc cagctggcgg acgtgccgct ccagcagatc
      901 acgccgacat gcgcatcggc cagcagccgc gccgcttcga ggatcttgct gtcgtcgaac
      961 tggcgcagcg aagcctcgcg cagcgagatc tcggtgacgg tgaagcgcgc gaagtgcgcc
     1021 gacacgcccg gcaagccgct gaccatggcg ctggtcagcg gttcgagcgc ggtgttcgac
     1081 gaaggggtga ggaccccgat cagtttgcgt tgtgccatgg tcggtgagga aggcgcttca
     1141 gacaggcagc ttcttgctgt agtcgatcag cagcgtcgag cagacgaaca ggcccgcgcc
     1201 ggcgacggcg aagagcgtca gcgccatgtc gtaggagccg gtcgcctgca cgatcaggcc
     1261 gaccacgatc ggcacgccga tgccggcgca gttgccgccc aggttcatgg tgccgcccag
     1321 gaagccgacg cggttgcgcg tgcccaggat ggagggaatg caccagaaca ggccgcacca
     1381 gcgcaggaag aacagcgtcg tcgacagcag gattaccact gtgaccgggt ccttgaagcg
     1441 cgccacgctg tagatggcga tggtggcgat caccgaggcg atgccgaaca gcgtgcgcag
     1501 caccttagcc tgcgacgcgc cggcggcctt ccacttgtcg gccagccagc cgcccaccat
     1561 ctcgccgacg aagccggaga agaacatgat gaacacggcg ccgcccatct gcttgatgtc
     1621 caggccgtgc accttggaca ggtaggtcgg catccaggtc agcaagccat agaacagcgc
     1681 gttgaagcac atccagccga agaacatgcc ccacaccgag cggtacttga agaagtccag
     1741 caccttgccg ctcacgttgg ccggctcggc cgcgtggtcg gcggcatggg ccgcctcgat
     1801 gtacgccgcc tcggcatcgt tgacggccgg gtgctggcgc gggtggttgc ggatgtacca
     1861 ccaggcgaag aggcccgcca ccatggtgcc gaggccggcc agcacaaagg aggtgcgcca
     1921 ggagccgaac tctgagatca gtccggcgat gatgagcgcg cccagcgccg cgcccagggg
     1981 cgcgccgcca tcgagcagcg tggcgccgcg gccgcgctcg ttctgcgtca tccagatgcc
     2041 gttgagcttg ccgccggcgg gataaatcgg cgcctcggaa gcccccagtc ccaggcgcgt
     2101 caccagcagg ctgacccagc cggtgcaggt cgcggcgaca gcctggaaga agccccagcc
     2161 cagcgtcgcg ccggcgatca ccacgcgcgg gccaaagcgg tcggccagca tgccgccggg
     2221 gatctgcatg aaggcatagg tccagaagaa ggcggacagc aacaggccct gcgtggccgg
     2281 tccgatgtcg aactccttcg cgatcaaggg catcgccacc gacagcgagg cgcggtcgat
     2341 gtagttgatc gagatgagga acagcatcat caggaagatc ttccatcgca cccgcgaggg
     2401 ctggatggct gagctgctga cgagcgtcgc cgcgttagct tgcatcgttt gtctcctagt
     2461 tgtgaacggc gccaaaacag cccttcgcac tgcagcaaag gaaccgtgcc gcaaatctag
     2521 gtgatgacga caataatgtc aaacatcttg tcaacaatga tgttgacaag atgttttctt
     2581 caggatcggg tggcgcagag gagcggggtt cgacttccga agaccagtta gcagcccggc
     2641 agcttcgagc tgatcgcggc cgatgtcggc cagggcacgg ccgtgctggt gcgcaccgcc
     2701 ggccatgcgc tgctctacga tgccggcccg cagtattcgc tcgacagcga tgccggcgat
     2761 cgcgtcctcg tgccgctgct gcgcggactc ggccagtcgc atctggaccg atttgatgct
     2821 gagccactgc gacaccgacc atgtgggcgg cgccgccgcc gtgatgaagg cgctgcgcgt
     2881 cgactcccag tggagctcgc tggagcccgg ccacgcgctg ctcggccagg gcatccccgc
     2941 cacgcgctgc gaggccggcc agggctggga atgggatggc gtgcgcttcg agctactgca
     3001 gccggcgccc gcgctctacg agcgcccagg cgtcaagccc aacgcgctga gctgcgtgct
     3061 gcgcgtgtcc ggcggcggcc gcagcgcctt gctgaccggc gatatcgagc gcgacggcga
     3121 agccgccatg gtggcggcct acggcgccgc gctgagcagc gacttgctga tcgtgccgca
     3181 ccatgggagc aagacttcct ccagcgccgc ctttctggat gcggtgcaac cccggattgc
     3241 tgtcatgcag gccggctacc gtaatcgcta cggccatccg tcgccggccg tgctcgcgag
     3301 ctatcgcgaa cgcggcattc atgtcatgct gagcgcgtct tgcggtgcct ggcgctggcg
     3361 gccgggtgtg cagagctgcc agcgcgacat cgcccggcgc tactggcagc acgtctggca
     3421 ccatccggat ctgccgcaat acgagggctc tggcctggaa cttgctgatc cagactaacg
     3481 actaggcgag aggcgaggag agcagcttgt ccatccatgt tgcgctgaat cacgtcacgc
     3541 actaccgtta cgaccggcgc gtgggcctgt caccccagat cgtgcgcctg cgccccgcgc
     3601 cgcattgccg cacccccatc ctgtcttact cgctgcgcat cgagccggtg cagcatttca
     3661 tcaactggca gcaggacccg ttcgcgaact acctggcgcg actggtgttc ccggaagaaa
     3721 ccaccgagtt caagatcacg gtggacctcg tggccgagat ggcggtctac aaccccttcg
     3781 acttcttcct cgaaccgggc gccgagcact atcctttcga ctacgagccg gggctggcgc
     3841 acgacctcgc gccctatctc gccaaggggc cgctgacgcc gcgcttcaag gccctgctcg
     3901 acagcatcag caccgacaag gtgcgcacca tcgacttcct ggtgagcctg aaccagcggc
     3961 tgcagcagga catccgctac ctcatccgca tggagcccgg cgtgcagacg ccggaagaga
     4021 cgctggagcg tgccgccggc tcgtgccgtg actccggctg gctgctggtg caggcgctgc
     4081 gccatctggg gctcgctgcc cgtttcgttt cgggctacct gatccaactc aagcccgatg
     4141 tgaaggcgct ggacggcccc agcggggcgg aagccgactt caccgacctg cacgcctggt
     4201 gcgaggtcta cctgcccggc gccggctgga tcgggctgga ccccacttcg ggcctgctgg
     4261 ccggcgaggg ccatatcccg gtggcctgca cgcccgagcc ttcgagcgcg gcgcctgtga
     4321 ccggcgaggt cgacgagagc gaggtcgagt tcggccacac gatgcaggtg acccgcgtct
     4381 tcgagtcgcc gcgcgtcacc aagccctata ccgaggagca atggcagcag gtgctggccc
     4441 tcggacatga ggtcgaccgc gggctcgaca agatggacgt gcggctcacg atgggcggcg
     4501 agcccacctt cgtttcggtg gacgaccgcg acggcgacga atggaacaca acagcgctcg
     4561 gccctaccaa gcgcggctac gcgacggagc tggtgcaaaa gctgcgcgcc aagtacgggg
     4621 ctggcggctt cctgcatttc ggccagggca agtggtatcc gggcg
//
LOCUS       DASUCY010000526         3434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_542375, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000526 DASUCY010000000
VERSION     DASUCY010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542375"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1282
                     /locus_tag="VFV25_10360"
     CDS             <1..1282
                     /locus_tag="VFV25_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY1393770.1"
                     /translation="TLATLAEAQPRGQWWQAFADPTLDDLVERAGRGNSSIQVAAARL
                     AQARALLRSAEADRAPQLDFAAGAERQDTPTTFNRPATLVSAGANLSYEVDLFGRLAR
                     ARDAAALDAQAREALLQSTRLLVQAEVAQTYLALRALDAERAIVRDTVEAYRNTLQLV
                     ERRNRAGDIAELDVVRVRTEVASTEAEALALDRQRATLEHALAVLVGEPASSFAVAEG
                     AWTTELPVVPAGVPGTVLARRPDVAAAQRQLEAAQARVGVARAAWFPSVSLTASGGYA
                     SPELSDLFKWSARAWSVGALMSLPVFDGGRRNAAVQSVGAELDAALASYREQVLVAFK
                     DVEDELSSLRLLAQQAEVQSSAVASAGRATTLSESRYRNGLVSQLDLLDAQRSELRNR
                     RQAVQVRGAQYQATVGLIRALGGGWGEPVSATGS"
     gene            1543..3264
                     /locus_tag="VFV25_10365"
     CDS             1543..3264
                     /locus_tag="VFV25_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010107534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEY1393771.1"
                     /translation="MKKTCTWVGIVSSALVLSACGSSNSDSNTSPLSSIGISHALLIS
                     IDGLHQQDVANCISSNTCPNIASLANTGVTYTGANTPGLSDSVPGLAALVTGGSPKTT
                     GLFYDDIYDRTLYSGVDLNCTGTQGVEVFLQEQVGIDGYNGGALAHLDGGGDFNPQQI
                     PRRKNASGACLPVYPHEFTQTNTIFEVVRQNIPGAFTAWADKHAWGTDWVNGPSGTGV
                     NDFARTEINSDYNTARGQTTPAGYDFTNSPTQTKVFDHYHTQIVINQIDGLDSTGVVS
                     AAMGGALQSNKIPVPTLFGTNYQTLSVSQKALNVNGGGYTDANFTPNTLTADAIKFLD
                     GEIGAIKTELANKNLLSSTVIIITAKHGQTPADRSKLRKIGDTIASTLTAANLGANSV
                     IGSGADAVTGNALGTGQFTEDDVGFIWLNSQSDAARASAVAALKANIGCPAVDPNTKI
                     IVAGQVNPGICAGVDAGSAVIDLKADGRFGDPTLGRTPDIMVQPNFGVIYSKSGKKDA
                     EHGGAASTDTGVGLIVSNPALAKQTVATQVLTTQVAPTIIQALKLDPNLLNSVKSEGT
                     AVLPNLF"
BASE COUNT          625 a         1192 c         1093 g          524 t
ORIGIN      
        1 gaccttggcg acgctggccg aggcacaacc gcgcggccag tggtggcagg ccttcgccga
       61 ccccacgctc gacgatctcg tcgagcgcgc cggccgcggc aacagcagca tccaggtggc
      121 cgccgcgcgg ctggcccagg cgcgcgcgct gctgcgcagc gccgaggccg accgcgcacc
      181 gcagctggac ttcgccgccg gcgcagagcg ccaggacacg cccaccacct tcaaccgccc
      241 cgccacgctg gtgagtgccg gggccaacct gtcttacgag gtcgacctct tcggccgcct
      301 ggcgcgcgcc cgcgacgcag ccgcgctgga cgcccaggcg cgcgaggccc tgctgcagag
      361 cacaaggctg ctggtgcagg ctgaggtggc gcagacctat ctcgcgctgc gcgcgctgga
      421 tgccgagcgc gccatcgtgc gcgacacggt ggaggcttat cgcaacacgc tgcagctcgt
      481 cgaacggcgc aaccgggccg gcgacatcgc cgagttggac gtggtgcgcg tgcgcaccga
      541 ggtggcctcc accgaggccg aggcgctcgc gctggaccgc cagcgcgcca cgctcgagca
      601 cgcgctggcg gtgctggtcg gcgagccggc atcaagcttc gccgtcgccg aaggagcctg
      661 gaccaccgag ctgccggtgg tgccggccgg cgtgcccggc accgtgctgg cgcgccgccc
      721 cgacgtggcg gccgcgcagc gtcagctgga ggcggcacag gcgcgcgtcg gcgtagcgcg
      781 cgcggcatgg ttccccagcg tgtcgctcac ggcctcgggc ggctatgcgt cgccggagct
      841 gtcggacctt ttcaaatggt cggcacgcgc gtggagcgtg ggggcgctga tgtcgctgcc
      901 ggtgttcgac ggcgggcgcc gcaacgccgc agtgcaaagc gtcggcgccg aattggacgc
      961 cgcgctggcc agctaccgcg agcaagtgct ggtggcgttc aaggatgtgg aagacgagct
     1021 gtcgtcgcta cgcctgctgg cccagcaggc cgaggtgcaa tcgagcgccg tggcctcggc
     1081 cgggcgcgcg accacgctgt cggaatcgcg ctaccgcaat ggcttggtga gccagctcga
     1141 cctgctggac gcgcagcgca gcgagctgcg caaccgccgc caggcggtgc aggtgcgcgg
     1201 ggcgcagtac caggccacgg tgggactgat ccgcgcgctc ggcggcggct ggggcgagcc
     1261 cgtctcggcc accgggtcct gatcgctccg gtccgagggc gggtagggcg tacctactcg
     1321 tcgaagaact tcggcttgcc gcggtcgtcg ccttatctgg cccggccaat tgctcctggc
     1381 ctatgtctgt tgccgttgac tccctgtcag cgagaggtca cgcacgggtc actgcaccga
     1441 aataacgtga gacgcccttt agcccacctg tgcggcatgc accacaggtg ggcgcaggca
     1501 ctgataagtc caacaaacag ccatagttag gagtctcaca gaatgaagaa gacctgcacc
     1561 tgggtcggca ttgtttccag cgcgcttgtg ctttccgctt gcggcagcag caacagcgat
     1621 agcaacacct ccccgctgtc ctcaatcggc atttcccacg cactgctgat cagcatcgac
     1681 ggtcttcacc agcaggacgt cgccaactgc atctcgagca atacctgccc gaacattgcc
     1741 agcttggcga acaccggcgt cacctatacg ggcgccaaca cgccgggcct gtcagattcc
     1801 gttcctggct tggccgctct ggtcaccggc gggagcccta agaccacggg tctgttctat
     1861 gacgacatct acgaccgcac gctgtattcc ggcgtggacc tgaattgcac cggcacgcaa
     1921 ggcgtcgagg tattcctgca agagcaggtc ggcatcgacg gctacaacgg cggtgccctt
     1981 gcgcatctgg acggcggcgg cgacttcaac ccgcagcaga ttccgcgcag gaagaacgcc
     2041 agcggcgcct gcctgccggt ctacccgcac gaattcaccc agaccaacac gatcttcgaa
     2101 gtggtgcgcc agaacatccc cggcgccttc accgcctggg cggacaagca cgcctggggc
     2161 acggattggg tcaacggccc ctcgggcaca ggcgtgaacg actttgcgcg caccgagatc
     2221 aattcggact acaacaccgc gcgcggccaa accacgccgg ccggctacga cttcaccaac
     2281 tcgccgaccc aaaccaaggt attcgaccac taccacaccc agatcgtcat caatcagatt
     2341 gacggcctgg attccacggg cgtcgtcagt gcggcaatgg ggggggcgct gcaaagcaac
     2401 aagatccccg tgcccacact tttcggcacc aactatcaaa ctctcagcgt gtcccagaaa
     2461 gcgctcaacg tgaatggcgg cggctacacc gacgccaatt tcacgcccaa tacgctgacc
     2521 gccgacgcca tcaagttcct cgatggcgag atcggcgcga tcaagactga gctagccaac
     2581 aagaatctgc tcagctcgac cgtcatcatc atcaccgcca agcacggcca gactccggcg
     2641 gaccggtcca agctccgcaa gatcggcgac accatcgcgt cgaccctgac ggcggccaat
     2701 ctgggtgcca acagcgtcat cggctccggc gccgacgccg tcacgggcaa cgcgctggga
     2761 accggccagt tcaccgagga cgacgtcggc ttcatctggc tgaacagcca gagcgatgcc
     2821 gcccgcgctt ctgcggttgc agcgctgaag gccaacattg gctgcccggc cgtggacccg
     2881 aacaccaaga tcatcgtggc gggccaagtc aatccgggca tctgcgccgg tgtcgatgca
     2941 ggaagcgccg tcatcgacct caaggctgac ggccgcttcg gcgaccccac cctgggacgc
     3001 acgccggaca tcatggtcca gcccaacttc ggcgtgatct acagcaagtc cggcaagaag
     3061 gatgcggagc acggcggcgc ggcgtcgacc gataccggcg tggggctgat cgtctccaat
     3121 ccggccctgg ccaagcaaac cgtcgcgacg caggtgctga ccactcaggt ggcgccgacg
     3181 atcatccagg cgctcaagct ggatcccaac ctgctgaact cggtcaagtc agaaggcacg
     3241 gccgtgctgc ccaacctttt ctgatcgcat cgacctctct ttgagagggc tcagagtcac
     3301 cttccaatcc ccgctcgacg gggattggtc atttgacgta ggtccaaagt gccagttgac
     3361 atgcaggtat tggcctgcat ataatcccct cacgcacaaa cgcaacggat ggacgcggac
     3421 aaggttttca aggc
//
LOCUS       DASUCY010000527         3390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_413546, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000527 DASUCY010000000
VERSION     DASUCY010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413546"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VFV25_10370"
     CDS             complement(<1..252)
                     /locus_tag="VFV25_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY1393772.1"
                     /translation="MIVLSDLPIGLAALTVLIIVLLAKSLRILREYERGVVFQLGRFW
                     KVKGPGLVILIPGVQQMVRIDLRVVVMDVPSQDVISRDNV"
     gene            complement(249..1700)
                     /locus_tag="VFV25_10375"
     CDS             complement(249..1700)
                     /locus_tag="VFV25_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HEY1393773.1"
                     /translation="MATSAFGAVAASLHRWAAMCLAAAAALLLPGAASSTEEAAAPPA
                     AAPVLVLRIDGAIGPATADYVRRGLSKAAREGAQLAVLEMDTPGGLDTAMRDIIKAVL
                     GSDVPVAGYVAPSGARAASAGTYILYASHVAAMAPASNLGAATPIAIGMPGGPSPAPE
                     EPSHPGAPASAASGAAQPEAPHDALTAKRVSDAAAYIRSLAQLRGRNADWAERAVREA
                     VSLPAADALAQHVVDLIAADLPDLLRQLDGRALKLADGRSVTLHTANAPTASYVPDWR
                     SRLLSVISDPSLALILMLIGVYGILFEFSNPGFVVPGVVGGLCLLLALYGLQTLPINY
                     VGLALILLGFAFFVAEAFVPSYGALGLGGVAAFGFGALMLFDTEAPGFGVPRALIFGL
                     SLVSAVFVIGIASMAARARRRPVVSGAAILVGATGELIESDAEGEGWATIAGERWRVR
                     TIGRLNPGQRLRVARVDGLTLEVQAIEVEGGQK"
     gene            complement(1741..1953)
                     /locus_tag="VFV25_10380"
     CDS             complement(1741..1953)
                     /locus_tag="VFV25_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin"
                     /protein_id="HEY1393774.1"
                     /translation="MSKHVYKLVELVGSSTTGTDDAIRNAIETAAQTLRKIDWFEVVE
                     TRGHVADGKVAHFQVTLKVGFRLEAS"
     gene            complement(2076..2546)
                     /locus_tag="VFV25_10385"
     CDS             complement(2076..2546)
                     /locus_tag="VFV25_10385"
                     /inference="COORDINATES: protein motif:HMM:NF020551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtaL-like protein"
                     /protein_id="HEY1393775.1"
                     /translation="MRFEHLVQINDAGNPLVPTMTRAELWRGLLARAWTPERFPLGPD
                     RCELRGGESVGLPLQRTVHFGGLALDDTVLAEPERSLRFEPAPREDMPKVVLTISIEE
                     PAPGDLFLRFLYESPEVAANKHELDLQGLRQQAWLANDLDMVRTLRQWLDEGLL"
     gene            complement(2678..>3390)
                     /locus_tag="VFV25_10390"
     CDS             complement(2678..>3390)
                     /locus_tag="VFV25_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1393776.1"
                     /translation="HVGWHAIFWFLTAVGLVLLAANWKVLPETLHDTHRQPFNLRHLV
                     RGYWELGSSARFLMLALASGIPFNGMFLYVLSAPVFLGEHLGLPPGQFFWFFTLTIAG
                     IMGGAWLSGRLAGRIKPRHQIRHGFLIMLVISLLNLGLNLAFKPEAWWALLPLAVYSF
                     GWALMVPVVTLMVLDLFPERRGMASSLQAFIGSVANGLVAGVIAPLVMHSTLALALAS
                     MVMMCVGLVAWLWVKPRL"
BASE COUNT          583 a         1166 c         1173 g          468 t
ORIGIN      
        1 gacgttgtcg cgcgagatca cgtcctgact gggcacgtcc atcaccacca cgcgcaggtc
       61 gatacgcacc atctgctgca cgccggggat gaggatcacg agcccggggc ctttgacctt
      121 ccagaagcgc ccgagctgga acaccacgcc gcgctcgtat tcgcgcagga tgcgcagcga
      181 cttggcgagc agcacgatga tcaacaccgt gagggccgcc aggccaatgg gcagatcgct
      241 cagcacaatc atttttgtcc tccttcgact tcgatggcct ggacttccag cgtgagcccg
      301 tccacgcgtg cgactcgcag gcgctggcct gggttcagcc tgccgatggt acgcaccctc
      361 cagcgctcgc ctgcgatcgt ggcccagccc tcgccttctg catcgctctc gatcagctcg
      421 ccggtcgcgc cgaccaggat ggccgcgccg gacaccacgg gccgccggcg cgcccgtgcg
      481 gccatgctgg cgatgccgat cacgaacacc gccgaaacca ggctcaggcc gaagatgagg
      541 gcgcgcggca cgccgaagcc cggcgcctcg gtgtcgaaaa gcatcagcgc gccgaagccg
      601 aaggcggcca cgccgcccag gcccagcgcg ccatagctgg gcacgaaggc ctcggcgacg
      661 aagaaggcga agccgagcag gatcagcgcc aggcccacgt agttgatcgg cagcgtctgc
      721 aggccgtaca aggccagcag caggcacagg ccgccgacca cgccgggcac gacgaagccc
      781 gggttggaaa actcgaagag gatgccgtag acgccgatca gcatcaggat cagcgcaagg
      841 ctggggtcgc tgatcaccga gagcaaacgg ctgcgccaat ccggcacgta gctcgcggtg
      901 ggcgcgttgg ccgtatgcag cgtgacgctg cggccgtccg ccagcttcag cgcgcgtcca
      961 tcgagctggc gcagcagatc gggcagatcg gcggcgatga ggtcgaccac atgctgggcc
     1021 agggcatcgg cggccggcag gctcaccgcc tcgcgcacgg cgcgctcggc ccagtcggca
     1081 ttacgcccgc gcagctgggc cagcgagcgg atgtaggccg ccgcgtcgct gacccgcttg
     1141 gcggtcagcg cgtcatgcgg cgcttctggc tgggcagccc cgctcgcggc cgaggccggc
     1201 gcgccggggt gcgacggctc ttcgggcgcg gggctgggcc ctccgggcat gccgatcgcg
     1261 atcggcgtcg cggcgcccag attgctggcc ggcgccatcg ccgcgacatg gctcgcatag
     1321 aggatgtaag tgccggcact ggccgcacgc gcgccgctgg gcgccacata gcccgccacc
     1381 ggcacgtcgg agccgagcac ggccttgatg atgtcgcgca tcgccgtgtc caggccgccc
     1441 ggcgtgtcca tctccagcac cgcgagctgg gcgccttccc gggcggcctt gctcaggccg
     1501 cggcgcacgt agtcggccgt ggccgggccg atcgcgccgt cgatgcgcag caccaacacg
     1561 ggcgcggcgg cgggaggcgc ggccgcctct tcggtgctcg atgccgcgcc gggcaacagc
     1621 aaggcggcag ccgccgccag gcacatcgca gcccagcgat gcagcgaggc tgcaacagcg
     1681 ccgaaagctg acgtggccat gttggcgccc gcggtcaagg tgcgctcagg ccccaggggc
     1741 ctagcttgcc tctagcctga atccaacctt tagcgtgacc tggaaatgcg cgaccttgcc
     1801 gtcggccaca tggccgcgcg tctcgactac ctcgaaccag tcgatcttgc gcaaggtttg
     1861 cgcggcggtc tcgatggcat tgcggatcgc atcgtcggtg cccgtggtcg acgagccgac
     1921 cagttcgacg agcttgtaga catgcttgga catgatcgcg tcctcctgcg gcctgccggt
     1981 agggcccggc gccctcagcg tacacccgcg agcgcgccgg gtcacgctgc atggcaggga
     2041 cgccgccgcc tattccgggg aggcgcggcg gcgcgtcaga gcagtccttc gtccagccat
     2101 tgccgcagcg tgcgcaccat gtccaggtcg ttggcgagcc aggcctgctg gcgcaggccc
     2161 tgcaggtcga gctcgtgctt attggccgcg acctcgggcg attcgtagag gaagcgcaga
     2221 aagagatcgc cgggcgccgg ctcttcgatg ctgatggtca gcaccacctt gggcatatct
     2281 tcgcgcggcg ccggttcgaa gcgcaatgag cgctcgggtt cggcaagcac cgtatcgtcc
     2341 agggccaggc caccgaaatg cacggtgcgt tgcagcggca gacccacgct ttcgccgccg
     2401 cgcagctcgc agcgatccgg gccgagcggg aagcgctcag gcgtccaggc gcgcgccagc
     2461 aggccgcgcc acagctcggc gcgcgtcatc gtgggcacca gcggattgcc ggcgtcgttg
     2521 atctggacca agtgttcgaa acgcatgggt tagcagctta ctcgcgaggc gctgcgacgg
     2581 ccgtgcagtt ctgtgcacta ctatttaagt agtgcatcgg cagaatattc aaggggccag
     2641 ggatgaattt cctgaaaatc atgcgggcgg tcgggcttca aagccgcggc ttcacccaca
     2701 gccaggcgac caggcccacg cacatcatca ccatcgaggc cagcgcgagc gccagggtgg
     2761 aatgcatcac cagcggcgcg atgacgccgg ccaccaggcc gttggcgacg ctgccgatga
     2821 aggcctgcag cgacgaggcc atgccgcgcc gctcggggaa gaggtccagc accatcagcg
     2881 tcaccaccgg caccatcagc gcccagccga aggagtacac cgccaggggc agcagcgccc
     2941 accacgcctc gggcttgaac gccaggttga ggccgaggtt gagcagcgag atcacgagca
     3001 tgatcaggaa gccgtggcgg atctggtgcc gcggcttgat gcggcccgcc agccgcccgg
     3061 acagccaggc gccgcccatg atgccggcga tggtgagcgt gaagaaccag aagaactggc
     3121 ccggcggcaa gccgaggtgc tcgccgagga acaccggcgc ggagagcacg tagaggaaca
     3181 tgccgttgaa gggtatgccg ctcgccagcg ccagcatcag gaagcgcgcg ctgctgccga
     3241 gctcccagta accgcgcacc aggtgccgca ggttgaacgg ctggcggtgg gtgtcgtgca
     3301 aggtctcggg cagcaccttc cagttcgcgg cgagcagcac gaggcccacc gcggtgagga
     3361 accagaagat cgcgtgccag cccacgtgca
//
LOCUS       DASUCY010000528         2710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_204862, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000528 DASUCY010000000
VERSION     DASUCY010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204862"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VFV25_10395"
     CDS             <1..314
                     /locus_tag="VFV25_10395"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEY1393777.1"
                     /translation="VVDEAGELVALQRMDKVQRASIDIAVGKARAAMLFLRPSAALEE
                     AAKARTNLLSVASDVPRVVFVQGGLPLIVGKDQVGAIGVSGLTSPQDEQVAKAGVDAI
                     K"
     gene            complement(333..854)
                     /locus_tag="VFV25_10400"
     CDS             complement(333..854)
                     /locus_tag="VFV25_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY1393778.1"
                     /translation="MEISAPCVVSLIWRLEDAQGQLIDELTEPVEFFYGGDDLLTKVE
                     EALAGQSEGFEAKLHLEPEQAFGDYDAQLVFFEDRKLFPEEVEPGMQFDGPPEGATTP
                     DLPSDLVYTVTEVYETHVVLDGNHPLAGIALRLALKVRAVREASETEIEQRSVAGSVV
                     TVLDTVPPPHRLH"
     gene            904..2085
                     /locus_tag="VFV25_10405"
     CDS             904..2085
                     /locus_tag="VFV25_10405"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY1393779.1"
                     /translation="MKKKAARTPVMDIDAVTPLLGGLSPRLFMRRHWQKKPLLIRQAL
                     PQVCSPVTRSELFALAAQEGVESRLVTQSAKGWMLRHGPLARRALPPLARAGWTLLVQ
                     GLEQHVPQARALLDRFRFVPEARLDDLMISYASDGGGVGPHFDSYDVFLLQVQGRRRW
                     RIGRLGDPQLLPDVPLKILSNFEAEEEWLLEPGDMLYLPPRWAHDGVAEGECMTCSIG
                     FRAPSRQELAREFLIRLAESLSEEEPLLYRDPAQLATVEPGLLPAELASFAQQALDLA
                     LRGRGGLLHTALGEYLSEPKRNVWFELGHPLSAGVGVTLDARTRMMFDDAHVYVNGES
                     WRAGGRDARLIRQLANARRLPAREVMRASQAARELLSEWAEDGWLHPLMEVEDGRDPS
                     R"
     gene            2063..2575
                     /locus_tag="VFV25_10410"
     CDS             2063..2575
                     /locus_tag="VFV25_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393780.1"
                     /translation="MDEIQAGELQPGNESFDTRSGFHAALKAAFAQAAQEGSRELWLC
                     DRDFADWPLGESAVVETLRQWADARRKLVLLAGTYDEFTRRHTRWLDWRLHWSHIADC
                     RMVDEQDTPVMPCILLSPNLVAVRRLDEFSSYRGRVYREGTDLARCREIIDAIAQRSE
                     PAFPATTLGL"
BASE COUNT          440 a          834 c          946 g          490 t
ORIGIN      
        1 cggtggtcga cgaggcgggc gaactggttg cactgcagcg catggacaag gtgcagcgcg
       61 cgagcatcga catcgcggtc ggcaaggccc gcgcggccat gctcttcctg cggccgagcg
      121 cggcgctgga agaggccgcg aaggcgcgca ccaatctgct gagcgtcgcg agcgatgtgc
      181 cgcgcgtcgt cttcgtgcag ggcggcttgc ccttgatagt gggcaaggac caggtcggcg
      241 cgatcggcgt cagcggcctc acgtcgccgc aggacgagca ggtcgcgaag gccggagtcg
      301 acgcaatcaa atagcgagct ctcggctgcg gatcaatgca ggcgatgggg cggcggtacc
      361 gtatcgagca ccgtcaccac cgagcccgcc accgagcgtt gctcgatctc ggtctcgctt
      421 gcctcgcgca cggcacgcac cttgagcgcc aggcgcagcg cgatgccggc cagcgggtgg
      481 ttgccgtcca gcaccacatg ggtctcgtag acctcggtga ctgtgtagac gaggtcgctg
      541 ggcaggtccg gcgtggttgc gccctccggc gggccgtcga actgcatgcc gggctcgacc
      601 tcctccggga agagcttgcg gtcttcaaaa aagaccagct gcgcgtcgta gtcgccgaag
      661 gcctgctcgg gctccaggtg cagcttggcc tcgaagccct cgctctggcc ggccagggct
      721 tcctcgacct tggtcagcaa atcgtcgccg ccgtagaaga actccaccgg ctcggtcagc
      781 tcgtcgatga gctggccttg ggcgtcttcg agccgccaga tgaggctgac gacgcaaggg
      841 gctgaaattt ccatgcgctg atcatctcat gagcgcagtg gggtgtcggc cacaatgcgc
      901 ggcatgaaga agaaggcagc ccgcacccct gtcatggaca ttgatgccgt cacgcccttg
      961 ctgggcggcc tctcgccgcg ccttttcatg cggcgtcact ggcagaagaa gccgctgctc
     1021 attcgccaag ccttgccgca ggtgtgctcg cccgtgacgc gcagcgagct gttcgcgctg
     1081 gccgcacagg aaggtgtgga gtcgcggctc gtgacgcaga gtgccaaggg ctggatgctg
     1141 cgtcacggtc ccttggcgcg gcgcgccctg ccgccgctag cgcgtgcggg ctggaccttg
     1201 ctggtgcagg gcctggagca gcatgtgccg caggcgcgtg cactgctgga tcgctttcgc
     1261 ttcgtgcccg aggcgcggct ggacgatctg atgatctcct atgcgagcga tggcggcggc
     1321 gtcgggccgc atttcgactc ctatgacgtc ttcctgctgc aagtgcaggg acgcagacgc
     1381 tggcgcatcg ggcgcctagg cgatccgcaa ttgctgccgg atgtgccgct gaagatcctc
     1441 agcaacttcg aggccgagga ggaatggctg ctggagccgg gcgacatgct ctacctgccc
     1501 ccgcgctggg cccacgatgg ggtcgccgag ggcgagtgca tgacctgctc gataggcttt
     1561 cgtgccccga gccgccagga gctggcgcgc gagttcctga tccggcttgc cgagtcgctg
     1621 agcgaagaag agccgctgct ttatcgcgac cctgcacagc tggcgacggt ggagcccggg
     1681 ctcctgccgg ctgaactggc gtccttcgcg cagcaggcgt tggatctggc gctgcgcggc
     1741 cgtggcggcc tgctgcacac cgccttgggc gaatatctca gcgagcccaa gcgcaatgtc
     1801 tggttcgagc tgggccaccc tctttcggcc ggggtgggtg tgaccctcga tgctcgcacg
     1861 cgcatgatgt tcgatgatgc gcatgtctat gtgaacggcg aatcctggcg cgccggcggg
     1921 cgcgatgcgc gactgatacg gcaactcgcc aacgcacgac gcctgccggc gcgcgaggtg
     1981 atgagagcga gccaggccgc gcgcgaactg ctgagcgaat gggccgagga cggctggctc
     2041 catccgctca tggaggtgga ggatggacga gatccaagca ggtgagttac agcccggtaa
     2101 cgagagcttc gatacgcgca gcggctttca cgcggcgctg aaggcggcat tcgcgcaggc
     2161 ggcgcaagag ggcagccgcg agctgtggct gtgcgatcgc gatttcgccg attggccctt
     2221 gggcgaaagc gcggtcgtgg aaacgctcag gcaatgggcc gacgctcgtc gcaagctggt
     2281 gctgctggcc ggcacttacg atgagttcac gcgacgccac acgcgctggc tggattggcg
     2341 gctgcactgg tcgcatatcg ccgactgccg catggtggac gagcaggaca cgccggtgat
     2401 gccttgcatt ttgctgtcac cgaatttggt ggcagtgcgt cgcctcgacg agttttcatc
     2461 gtatcgaggg cgcgtttaca gagagggaac ggatctggca cgctgccgcg aaatcattga
     2521 cgcgattgcg caacgctcgg agccggcttt tccagcgacc accttgggcc tgtaacgaac
     2581 ccggcaggtc agcacgtata atgttaggct aggcttggaa atgctcgagc gttcctcgtc
     2641 ttcgttggga ctcgaagatc tttcggagag ttccgtcaat taccggtaat tgtccgactc
     2701 tttgattgag
//
LOCUS       DASUCY010000529         3805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_879465, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000529 DASUCY010000000
VERSION     DASUCY010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879465"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..680)
                     /locus_tag="VFV25_10415"
     CDS             complement(171..680)
                     /locus_tag="VFV25_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEY1393781.1"
                     /translation="MITSLRSALLRMFGPVLAGLAFANAGAAVPSGVAPDFTLRTLGG
                     QNLRLQEQRGQVVLVNFWATWCGPCRQEMPHLNSLYEKYRTSGFVLLGVNVDDDARNA
                     VGVASKLGLKFPVLFDTDKKVSKLYELVTMPSTVLIDRDGRVRYVHRGYLDGYADTYD
                     KQIRELLKE"
     gene            complement(696..1358)
                     /locus_tag="VFV25_10420"
     CDS             complement(696..1358)
                     /locus_tag="VFV25_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel domain-containing
                     protein"
                     /protein_id="HEY1393782.1"
                     /translation="MRSSQLLPSTLVAACGAVLFIALPPARAAEQQQTTDEQQVIVPQ
                     VDRREVRLPHYPSNDFEAGLFTGTYATQNFGSSLVGGVRLGYHLTEDFFAEAVYAQTK
                     VSDSSFRQVLPGGVFPQPREWLKYYNLSVGYNLLPGEVFFGNKVAKASAFYVIGGLGS
                     TKFNDQSRQTINLGLGMRLFLRDWAAVQVDMRDHIFSLDLLGKRQNTQNLELTGGFTF
                     FF"
     gene            complement(1382..2167)
                     /locus_tag="VFV25_10425"
     CDS             complement(1382..2167)
                     /locus_tag="VFV25_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEY1393783.1"
                     /translation="MSFLAVSQSLRRGGRACVVAVATLAACAFAPIAQADEAVERVQI
                     TDPYIELHTGPGRGYPVLFVAAREEWIEILLRHTDWFKVRTAGGKEGWVQRQQLATTL
                     TEAGERKSFRDIALDDYLSRRLELGASWGHFKSEPMLKIWSAYRLSDTLSIEATVGQV
                     QGLFSGTDFWHFNLNEEPWSDQRLSPFFGIGVGKFKNFPNQSLVGATTTDAKLANAAI
                     GARYHLNERFVLRADYSIYTAFIADSRTGEYRALTLGLSFFFQ"
     gene            complement(2164..2775)
                     /locus_tag="VFV25_10430"
     CDS             complement(2164..2775)
                     /locus_tag="VFV25_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393784.1"
                     /translation="MTLQLRVLGALFGFATAALTGCGGGANPLGNPPLVSNPAGASGQ
                     SLSFIYYQKCINPIFLARLQINLNGVVSTNTCAGSGCHDNVTGTGGAFRIVPSAQPVD
                     LSNPANTPAVIRASDMYKNFYSAQGEVDFNAPAQSRLLIKPQLLGVLHGGGLIFASAQ
                     DPNLKLIEYWINHPMPQGQDEFSTAAYSMFTPADPNTGTCNTQ"
     gene            complement(2772..>3805)
                     /locus_tag="VFV25_10435"
     CDS             complement(2772..>3805)
                     /locus_tag="VFV25_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393785.1"
                     /translation="ETMAATRARLDPSVLSLVADMNYTDVWADTSQAGVTARPSIALR
                     CTGNPNPADDLATPVPTNGIVNYPDHIQPIWSRSRGANTCTNCHTSADTVLNLSATIA
                     GSGRMASYDRLMIGDPLLDPKTGLPQVQIEDGVPVIMRKPALVDTEANESMALGLARK
                     SRLIEIMFGQSLMAGADAIAAHPNPPSSAPNHASMLNAAEKRLVTEWIDLGGKYYNNP
                     FASGSTVRSVNTLNQATFVAQVYPILRTTCAAGCHQAIGSDKTTPAGTSFRENRLVLT
                     GDPVGDFNVTLTMISDTCHPANNYLLSKPSTIPHPAGATGQTTAVLPAGSAGYTAISN
                     WIASGCPTP"
BASE COUNT          611 a         1153 c         1356 g          685 t
ORIGIN      
        1 gcgatgcttc tcgggcagcg tgtcgcgcga gaacttcatg atgggatcgg cgaggcgctc
       61 gcgctcatag ggcttgaccc agggctcgac gccgcaggcc gagagcaatg cggcgcccgc
      121 cagcatggcg agcagcgtgg ccgaagagag tgatcgctgc atgggcttct tcattccttc
      181 agcaactccc ggatctgctt gtcataggtg tcggcgtagc cgtccagata gccgcggtgc
      241 acgtagcgca cccggccgtc gcggtcgatc agcacggtgg acggcatggt cacgagctcg
      301 tagagcttgc tgaccttctt atcggtgtcg aacagcaccg ggaacttcag gccgagcttg
      361 gaagccacgc cgactgcgtt gcgcgcgtcg tcgtccacgt tgacgcccag cagcacgaag
      421 cccgaggtgc ggtatttctc gtagaggctg ttcagatgcg gcatctcctg gcggcagggg
      481 ccgcaccagg tggcccagaa attgaccagc acgacctggc cgcgctgctc ctgcaggcgc
      541 aggttctggc cgcccagggt gcgcagcgtg aagtcgggcg cgacgccgct gggcacggcc
      601 gcgccagcgt tcgcaaaggc aaggccggcc agcaccgggc cgaacatgcg cagcaaggcg
      661 gatcgcaggg aggtgatcat cgtgtgagcc cctgttcaga agaagaaggt gaagccaccg
      721 gtcagctcga ggttctgcgt gttctggcgc ttgcccagca ggtccagcga gaagatgtgg
      781 tcgcgcatgt cgacctgcac cgcggcccag tccctcagga agagccgcat gcccaggccc
      841 agattgatgg tctggcggct ctggtcgttg aacttggtgc tgcccaggcc gccaatgaca
      901 tagaaggccg aggctttggc gaccttgttg ccgaagaaga cctcgcccgg cagcaggttg
      961 tagcccaccg aaaggttgta gtacttcagc cattccctgg gctgggggaa gacgccgccg
     1021 ggcagcacct ggcgaaacga ggaatcgctg accttggtct gggcatagac cgcctcggcg
     1081 aagaaatcct cggtgaggtg atagcccagg cgcacgccgc caaccaggct ggagccgaag
     1141 ttctgcgtgg cataggtgcc ggtgaagagc ccggcctcga agtcgttgga cgggtagtgc
     1201 ggcaggcgga cttcgcgtcg atccacctgc ggcacgatga cttgctgttc gtccgtggtc
     1261 tgctgctgct ctgctgcccg tgctggcggc agcgctatga acagcacggc gccgcaggcg
     1321 gcgaccaggg tcgaaggaag gagttggctt gagcgcattg ctttggtctc tggtgtcgtt
     1381 gttattggaa gaagaacgag agccccagcg tcagcgcccg gtactcgccg gtgcgcgagt
     1441 ccgcgatgaa ggcggtgtag atcgagtagt cggcgcgcag cacgaagcgc tcgttcaggt
     1501 ggtagcgcgc gccgatggcg gcattggcca gcttggcatc ggtggtggtc gcgccgacca
     1561 ggctctggtt cgggaagttc ttgaacttgc cgacgccgat cccgaagaag ggcgacaacc
     1621 gctggtcgga ccagggctcc tcgttgaggt tgaagtgcca gaagtcggtg cccgagaaaa
     1681 ggccctgcac ctggcccacg gtggcttcga tcgacaaggt gtccgagagc cggtaggcgc
     1741 tccagatctt gagcatgggc tcggacttga agtgccccca ggaggcgccc agctccagcc
     1801 gccggctcag atagtcgtcc agcgcaatat cgcgaaagct cttcctctcg cccgcttcgg
     1861 tcagcgtggt cgcgagctgc tggcgctgca cccagccttc cttgccgccg gctgtgcgga
     1921 ccttgaacca atcggtgtgg cgcagcagga tctctatcca ttcctcgcgc gccgcgacga
     1981 ataagaccgg atagccacgg cccgggccgg tgtgcagctc gatgtaggga tcggtgatct
     2041 gcacccgctc cacggcctcg tcggcctggg cgatgggcgc aaacgcgcat gcagccaagg
     2101 tcgcgacggc aacaacgcac gcgcgccccc cgcgccgcag tgactggctg acggcgagga
     2161 agctcattgc gtgttgcagg tgccggtgtt gggatcagca ggcgtgaaca tgctgtaggc
     2221 cgccgtgctg aattcgtcct gcccctgggg catcggatgg ttgatccagt actcgatcag
     2281 cttcaggttg gggtcctggg cactggcgaa gattaggccg ccgccgtgca gcacgcccag
     2341 cagctggggc ttgatcagca accggctctg cgccggcgca ttgaaatcca cctcgccttg
     2401 cgccgagtag aagttcttgt acatgtcgct ggcgcggatc acggccggcg tgttggcggg
     2461 gttgctgaga tcgaccggct gcgcgctcgg cacgatgcgg aaggcgccgc ccgtgccggt
     2521 cacgttgtcg tgacagcccg agccggcgca ggtgttggtc gacacgacgc cgttcagatt
     2581 gatctgcagg cgcgccagga agatagggtt gatgcacttc tggtaataga tgaaggacag
     2641 cgactgcccc gaggcgccgg ccggattgct gaccagcggc ggattgccga gcggattcgc
     2701 cccgccaccg cagccggtca aggccgctgt cgcgaagccg aacagcgcgc caagaactct
     2761 cagttgaagc gtcatggtgt ggggcaaccg ctcgcgatcc agttggagat ggccgtgtag
     2821 cccgcgctgc ccgctggcag caccgccgtg gtctgacccg tggcgccggc cggatgcggg
     2881 atcgtcgacg gcttgctgag caggtagttg ttggccgggt ggcaggtgtc ggaaatcatc
     2941 gtcagcgtga cgttgaagtc gcccaccgga tcgccggtca gcaccagccg gttctcgcgg
     3001 aaggaggtac ccgccggcgt cgtcttgtcg ctgccgatgg cctggtggca gccggccgcg
     3061 caggtggtgc gcaggatcgg gtagacctgg gccacgaagg tggcctggtt cagcgtgttg
     3121 acgctgcgca ccgtgctgcc gctggcgaag gggttgttgt agtacttgcc gcccaggtct
     3181 atccattcgg tgaccaggcg cttctcggcc gcgttgagca tgctcgcgtg gttgggcgcg
     3241 ctgctcggtg gattgggatg ggcggcgatc gcgtcggcgc cggccatcag gctctggccg
     3301 aacatgatct cgatcagccg gctcttgcgg gccaggccga gcgccatgct ctcgttggcc
     3361 tcggtatcca ccagcgccgg cttgcgcatg atgaccggca caccgtcctc gatctggact
     3421 tgcggcaggc cggtcttggg gtcgagcagg gggtcgccga tcatcagcct gtcgtaggag
     3481 gccatgcgcc cgctgccggc gatcgtggcg ctcaggttga gcacggtgtc cgcgctggtg
     3541 tggcagttgg tgcaggtgtt ggcgccgcgg ctgcgcgacc atatcggctg gatatggtcg
     3601 gggtagttga cgatgccgtt ggtcggcacc ggcgtggcga ggtcgtcggc cggattcggg
     3661 ttgccggtgc agcgcagagc aatcgacggg cgtgccgtaa cgccggcctg gctcgtgtcg
     3721 gcccagacat cggtgtagtt catgtcggcc accaggctca gcacgctggg gtcgaggcgg
     3781 gcgcgcgtcg cggccatggt ctcgc
//
LOCUS       DASUCY010000530         3007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_767318, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000530 DASUCY010000000
VERSION     DASUCY010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767318"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="VFV25_10440"
     CDS             <1..919
                     /locus_tag="VFV25_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY1393786.1"
                     /translation="KKLLSEQKVDAIIGPSGSPNALGMIQFAAEAHTPLLAPVGAVAV
                     VQPMDENKRWVFKPTQNDELIAQALVAHMRKTGVKTLGFIGLNDAYGESWHRVMLPLA
                     EQAGIKVVTDERYARTDQSVLGQAVKIVAARPDAVLVAAAGGPTVLPQTTLVERGYKG
                     AVYQTHGAATPDFIRLGGKAVEGTVMAASLMLVLDQVSDSHPAKKVALDYIAAYERRY
                     GTRPATFGANVYDAGLLLQHAVPEAAKKARPGTPEFRSALRDALERTRDLVGTQGVYN
                     MSALDHSGFDQRGRVLMTLKNGSWQILPE"
     gene            1009..2349
                     /locus_tag="VFV25_10445"
     CDS             1009..2349
                     /locus_tag="VFV25_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007839076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS family transporter"
                     /protein_id="HEY1393787.1"
                     /translation="MTDGTLAATTNNDDSPEERRRRIWAIVGGSSGNLVEWYDFYVYS
                     FTALYFAAEFFPKGDQTSQLLNTAGVFAAGFLMRPIGGWLFGRIADRHGRRTSMMISV
                     LMMCGGSLMVAVLPTYASIGTLAPVLLLLARLIQGVSVGGEYGTSATYMSEVAISGRR
                     GFYSSFQYVTLIGGQLLAVLVLVILQQFLSTEELKAWGWRIPFVIGALAAVVALFLRR
                     SLHETTSAQVRGEQHAGTIRGILRYPRSLLTVLGFTAGGSLAFYTFTTYMQKYLVNTA
                     GMSAKTASAVMTAVLLCYMLMQPVFGALSDRIGRRNSMLCFGTLSVLTTVPLLSALGQ
                     VSSPYAAFGLVLLALAVISFYTSISGLVKAELFPMEVRAMGVGLSYAVANALFGGSAE
                     YVALWFKSAGHESTFFWYVTVMTGIALIASITMPDTRRAGYMKAREGTLTPATS"
     gene            2409..>3007
                     /locus_tag="VFV25_10450"
     CDS             2409..>3007
                     /locus_tag="VFV25_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY1393788.1"
                     /translation="MKFKALAVACSILLAGAVTAAHASGPDVVIGDIDDLSGLYADVV
                     GAGGIEAAKMAITDFGGMVLGRKIVLLVADHQNKPDIGASKFREWADQNGVSMVLGGS
                     NTGVNIAMSKVAGAKKVPFISIGAAGASLTNQDCTPYSVHYAYDTTALANGTATAIVA
                     AGGKTWFYLTADYAFGTQLQTAASKVVETHGGKNLGAVRV"
BASE COUNT          499 a         1017 c          991 g          500 t
ORIGIN      
        1 gaagaagctg ctgagcgagc agaaggtcga cgcgatcatc ggcccttcgg gctcacccaa
       61 tgcgctgggc atgatccagt tcgcggccga ggcgcacacg ccgctgctgg cgccggtggg
      121 cgcggtggcc gtcgtgcagc ccatggacga gaacaagcgc tgggtcttca agcccacgca
      181 gaacgacgag ctgatcgccc aggccctggt ggcgcatatg cgcaagacgg gcgtgaagac
      241 cctgggcttc atcggcctca acgatgccta tggcgagtcc tggcatcgcg tgatgctgcc
      301 gctggccgag caggccggca tcaaggtcgt caccgacgag cgctatgccc gcaccgatca
      361 gagcgtgctg ggccaggccg tgaagatcgt cgcggcccgg cccgatgcgg tgctggtcgc
      421 ggcagccggc ggcccgacgg tgctgccgca gaccacgctg gtcgagcgcg gctacaaggg
      481 cgcggtctac cagacgcatg gcgccgcgac gccggatttc atacgcctgg gcggcaaggc
      541 tgtcgaaggc acggtcatgg cggcgagcct gatgctggtg ctggaccagg tgagcgacag
      601 ccaccccgcc aagaaggtcg cgctcgacta catcgcggcc tatgagcggc gctacggcac
      661 caggccggcc accttcggcg ccaatgtcta cgacgcaggc ctgctgctgc agcacgccgt
      721 gcctgaggca gctaaaaagg ccagacccgg cacgccggaa ttccgcagcg ccctgcgcga
      781 cgcgctggag cgcacccgtg atctggtcgg cacccagggc gtctacaaca tgagcgcgct
      841 ggatcattcc ggcttcgacc agcgcggccg cgtgctgatg acactgaaga acggcagctg
      901 gcagatcctg cccgaatagg tttcgctgcg aattgacgaa accccggctc cggctgatag
      961 tgcgtcgcac agcggggatg cccccgcgac atcacccgga gatcaagaat gacggatggg
     1021 acactagctg ccacgaccaa caatgacgac agccccgagg agcgccgccg gcgcatctgg
     1081 gccatcgtcg gcggctcctc gggcaacctg gtcgagtggt acgacttcta cgtctactcc
     1141 ttcaccgcgc tctacttcgc ggccgagttc ttccccaagg gcgaccagac cagccagttg
     1201 ctgaacactg ccggcgtctt cgcggccggc ttcctgatgc gcccgatcgg cggctggctg
     1261 ttcggccgca tcgccgaccg gcacggccgg cgcacttcga tgatgatttc ggtgctgatg
     1321 atgtgcggcg gctcgctgat ggtggccgtg ctgcccacct atgccagcat cggcaccctc
     1381 gccccggtgc tcctgctgct ggcccggctg atccagggcg tctcggtggg cggcgaatac
     1441 ggcaccagcg ccacctacat gagcgaggtg gcgatctcgg gccggcgcgg cttctattcg
     1501 tccttccagt acgtcacgct gatcggcggg cagctgctcg ccgtgctggt gctggtcatc
     1561 ctgcagcagt tcctctcgac cgaggaactc aaggcctggg gctggcgcat ccccttcgtg
     1621 attggagcgc tagccgcggt cgtggcgctc ttcctgcgcc gctcgctgca cgagaccacc
     1681 tcggcccagg tgcgcggcga gcagcatgcc ggcacgatac gcggcatcct gcgctacccg
     1741 cgctcgctgc tgaccgtgct gggcttcacc gcgggaggct cgctggcctt ctacaccttc
     1801 acgacctaca tgcagaagta tttggtcaac acggccggca tgagcgccaa gaccgccagc
     1861 gcggtgatga ctgcggtgct gctgtgctac atgctgatgc agcccgtgtt cggcgcgctg
     1921 tccgaccgca tcggccggcg caactcgatg ctgtgcttcg gcaccctgag cgtgctgacg
     1981 acggtgccgc tgctgagcgc gctcggccag gtcagcagcc cctatgcggc cttcggcctg
     2041 gtcttgctgg cgctggccgt gatcagcttc tacacctcga tcagcggcct ggtgaaggcc
     2101 gagctgttcc cgatggaggt gcgtgcaatg ggcgtggggc tgtcctatgc cgtggccaac
     2161 gccctcttcg gcggcagcgc tgaatatgtg gcgctgtggt tcaagtcggc cggccatgag
     2221 tcgaccttct tctggtacgt gacggtgatg acgggcatcg cgctgattgc atcgatcacg
     2281 atgccggaca ccaggcgggc cggctacatg aaggcacgcg aggggactct gacgccggcg
     2341 acgagctgag aagaagacag cactcgcgcc gccttctgga acgaccgacc atccaacgag
     2401 gagaacgcat gaagttcaag gccctggctg ttgcttgctc gatcctgctc gcgggtgccg
     2461 tcaccgcggc ccacgcatcc ggtcccgatg tggtgatcgg agacatcgac gatctctccg
     2521 gcctctatgc cgacgtggtc ggcgcaggcg gcatcgaggc cgcgaagatg gcgatcaccg
     2581 atttcggcgg catggtgctg ggccgcaaga tcgtgctgct cgtcgccgat caccagaaca
     2641 agcccgatat cggcgcctcc aagttccgcg aatgggccga ccagaacggc gtcagcatgg
     2701 tgctaggcgg ctccaacacc ggcgtcaaca tcgcgatgtc caaggtggcg ggcgccaaga
     2761 aggtgccctt catctcgatc ggcgcggcag gcgcctcgct caccaaccag gattgcacgc
     2821 cctactccgt ccactacgcc tacgacacga cggccctggc caacggcacc gccaccgcga
     2881 tcgtcgccgc cggcggcaag acctggttct atctgaccgc cgactacgcc ttcggtaccc
     2941 agctgcagac cgccgcctcg aaggtggtcg agacccatgg cggcaagaac ctcggcgcgg
     3001 tgcgcgt
//
LOCUS       DASUCY010000531         2280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1152941, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000531 DASUCY010000000
VERSION     DASUCY010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152941"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="VFV25_10455"
     CDS             <1..697
                     /locus_tag="VFV25_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEY1393789.1"
                     /translation="TIGYVKGRPYSPRGVEWEQAVAYWRTLQSDPDAHWDRVVELDAS
                     QIRPQVTWGTSPEMVLSIEDRVPDPEREKDPVKRNAMERALQYMALEPNKAIGDIRID
                     KVFIGSCTNSRIEDMREAAAVVRRLGGKVASNVKLAMVVPGSGLVKEQAEREGLDQVF
                     KAAGFEWREPGCSMCLAMNADRLEPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAA
                     AMQGHFVDVRRIA"
     gene            733..1380
                     /gene="leuD"
                     /locus_tag="VFV25_10460"
     CDS             733..1380
                     /gene="leuD"
                     /locus_tag="VFV25_10460"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEY1393790.1"
                     /translation="MQAFRIHTGLVAPIDRENVDTDAIIPKQFLKSIKRSGFGPNLFD
                     EWRYLDAGQPGQDPASRRPNPDFVLNQPRFQGASVLLARRNFGCGSSREHAPWAIEQY
                     GFRALIAPSYADIFFNNCFKNGLLPLTLPEHEVTKLFDEVAAFPGYKLTIDLERQVVV
                     KPDGSELGFDVQPFRKYCLLNGFDDVALTLRYADKIRAFEAERLARQPWLNQRIV"
     gene            1385..1663
                     /locus_tag="VFV25_10465"
     CDS             1385..1663
                     /locus_tag="VFV25_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393791.1"
                     /translation="MRQRLTHWLRRKPWALLLGLGLCLLGFGVVSLNLFFSLSANLTL
                     IREHGWMALRDGAARQLFELLGQGVLATLFYAGLKVFERLIVEWWLAR"
     gene            1689..>2280
                     /locus_tag="VFV25_10470"
     CDS             1689..>2280
                     /locus_tag="VFV25_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEY1393792.1"
                     /translation="MKIAVLPGDGIGTEIVAEAVKVLGALDLKFELEEAPVGGAAYEA
                     VGHPLPEATLKLAQAADAVLFGAAGDWKYDKLERALRPEQAILGLRKHLGLFANFRPA
                     ICYPELTHASSLKPELVAGLDILIIRELTGDIYFGQPRGRRTAVDGHFPGAEEAFDTM
                     RYSRPEIERIAHVAFQAARKRSKRVTSVDKANVLETF"
BASE COUNT          375 a          782 c          746 g          377 t
ORIGIN      
        1 caccatcggc tatgtgaaag ggcgccccta ttcgccgcgc ggcgtcgagt gggagcaggc
       61 cgtggcctat tggcgcacgc tgcagtccga ccccgatgcg cattgggacc gcgtggtcga
      121 gctcgatgcc tcgcagatcc gcccgcaggt cacctggggc acctcgcccg agatggtgct
      181 gtccatcgaa gatcgcgtgc ccgatcccga gcgcgagaag gatccggtca agcgcaacgc
      241 gatggagcgc gcgctgcagt acatggcgct ggagcccaac aaggcgatcg gcgacatccg
      301 catcgacaag gtcttcatcg gctcctgcac caattcgcgc atcgaggaca tgcgcgaggc
      361 cgccgcagtg gtgcgccgcc tgggcggcaa ggtggcgtcc aacgtgaagc tggcgatggt
      421 ggtgcccggc tccggcctcg tgaaggagca ggccgagcgc gaaggcctgg accaggtgtt
      481 caaggccgcc ggcttcgaat ggcgcgagcc cggctgttcg atgtgcctgg cgatgaacgc
      541 cgatcgcctg gagcccggcg agcgctgcgc ttcgacgagc aaccgcaatt tcgaaggccg
      601 ccagggtgcg ggcgggcgca cccacctcgt cagccccgcg atggccgctg ctgccgcgat
      661 gcagggccat ttcgtcgacg tgcgccgcat cgcctgaggc gagcaagcaa gacttaaacc
      721 tcgagttcac tcatgcaagc cttccgcata cacaccggcc tggtggcgcc catcgaccgc
      781 gagaacgtcg ataccgacgc catcatcccc aagcaattcc tgaagtccat caagcgcagc
      841 ggcttcggcc ccaatctctt cgacgagtgg cgctatctcg acgcgggtca gccggggcag
      901 gatccggcct cgcgccgccc caatcccgac ttcgtgctca accagccgcg ctttcagggc
      961 gcctcggtgc tgctggcgcg gcgcaatttc ggctgcggct ccagccgcga gcacgcgccc
     1021 tgggccatcg agcaatacgg cttccgcgcg ctgatcgcgc cgagctatgc cgacatcttc
     1081 ttcaacaact gcttcaagaa cggcctgctg ccgctcacgc tgcccgagca tgaagtgacc
     1141 aaactgttcg acgaggtcgc cgcctttccg ggctacaaac tcaccatcga cctggagcgc
     1201 caagtcgtgg tcaagcccga cggcagcgag ctgggcttcg acgtgcagcc cttccgcaag
     1261 tactgcctgc tcaacggctt cgacgacgtt gcgctgaccc tccgctatgc cgacaagatc
     1321 cgcgccttcg aggccgagcg gctggcgcgc cagccctggc tcaaccagcg catcgtctga
     1381 gcgcatgcgc cagcgcctga cgcattggct gcggcgcaag ccctgggcct tgctgttggg
     1441 cttggggctg tgcctgctcg gcttcggtgt cgtttcgctg aacctgttct tttcgctgtc
     1501 ggccaacctc acactgattc gcgagcacgg ctggatggcg ctgcgtgacg gcgccgcgcg
     1561 gcagctgttc gagctgctgg gccagggtgt gttggcgacg ctcttctatg caggcctcaa
     1621 ggtgttcgag cgcctgatcg tcgaatggtg gctggcccgc tgaggctgcc gccgcaacaa
     1681 ggggaatcat gaagatcgca gtcttgccgg gtgacggcat cggtaccgaa atcgtcgccg
     1741 aggccgtaaa ggtgctcggg gcgctggacc tgaagttcga gctggaagag gcgcccgtgg
     1801 gcggagctgc ctacgaggcc gtgggccacc ccctgcctga ggcgacgctc aagctggccc
     1861 aggcggccga cgcggtgctg ttcggcgccg cgggcgattg gaaatacgac aagctggagc
     1921 gcgcgctgcg ccccgagcag gccatcctgg gtttgcgcaa gcacctgggc ctgttcgcca
     1981 acttccgccc ggcgatctgt tatccggagc ttacgcacgc ctccagcctc aagcccgagc
     2041 tggtggccgg gctggacatc ctcatcatcc gcgagctaac cggcgacatc tacttcggcc
     2101 agccgcgcgg ccgccgcacc gcggtcgacg ggcacttccc cggcgcggag gaagccttcg
     2161 acaccatgcg ctactcgcgc ccggagatcg agcgcatcgc ccatgtggcc ttccaggcgg
     2221 cgcgcaagcg cagcaagcgc gtgaccagcg tcgacaaggc caatgtgctg gagaccttcc
//
LOCUS       DASUCY010000532         2431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_879513, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000532 DASUCY010000000
VERSION     DASUCY010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879513"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VFV25_10475"
     CDS             complement(<1..466)
                     /locus_tag="VFV25_10475"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY1393793.1"
                     /translation="MPDSPSDSASSAHPESHPEPTRIIAIRHGETAWNVEARIQGQLD
                     IPLNDTGRWQAHRLALALAEEDVAALYASDLLRAYETAQAASRGTSLAINTDTGLRER
                     GFGIFQGMTYEEVDQQWPEQAARWRRRDPDFGPEGGETLRDFYARSVATAERL"
     gene            complement(491..1120)
                     /locus_tag="VFV25_10480"
     CDS             complement(491..1120)
                     /locus_tag="VFV25_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEY1393794.1"
                     /translation="MKLTSSSWTDGQRIPAQYAAGKHDAQGGVGFSDNLNPHLAWNDV
                     PAGTMSFALICHDPDVPSRGDDVNKPDREVPASLPRVDFFHWVMVDLPPALREIAESE
                     FSRGFTARGKSGPATLHGARHGINDYTAWFAGDAQMGGQYYGYDGPFPPFNDTLLHHY
                     VFTLYALDLQRLAVEGEFTGPQVREAMRGHVLAEASFSGIYSLNKRLLG"
     gene            complement(1152..1478)
                     /locus_tag="VFV25_10485"
     CDS             complement(1152..1478)
                     /locus_tag="VFV25_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1840 domain-containing protein"
                     /protein_id="HEY1393795.1"
                     /translation="MLYKFKSKAAGDLIMLGASGAQLLGIIGKQPAAKGIIEPQAMPA
                     AMQAIKDAIAADEAVRAKAEAEALAAGKPPPHWEGVISLRQRAWPMVEMMKLALAADE
                     PIVWGV"
     gene            complement(1492..>2431)
                     /locus_tag="VFV25_10490"
     CDS             complement(1492..>2431)
                     /locus_tag="VFV25_10490"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY1393796.1"
                     /translation="GKSLARHTRERDAQQIQFHYDVSDDFYALWLDPRRVYSCAYYRD
                     PTMSLAQAQEAKLDHICRKLMLEPGMRFLDIGAGWGGLLLWAAEHYGVEAHGVTLSKN
                     QHAHVNRLIEEKGLQGRVRMELCDYRDIGEDEPYDRIASVGMFEHVGRARLVGYFEKI
                     RSLLKPGGLLMNHGITAGGLHNSQLGAGMGDFMERYIFPGGELVHVSEVMRAMAEAGL
                     EALDVENLRPHYARTLWAWSDALEAQLDRARQLTRESVVRAYRLYLAGSAMSFEQGWV
                     ALHQMLASRPSGVVTDGEALRGAQSAYPFNRGYIYS"
BASE COUNT          341 a          795 c          866 g          429 t
ORIGIN      
        1 ccaggcgctc ggcggtggcg acgctgcggg catagaagtc gcgcagggtc tcgccgcctt
       61 cggggccgaa gtcggggtcg cgccggcgcc agcgcgcggc ctgctcgggc cattgctggt
      121 cgacctcctc ataggtcatg ccctggaaga tgccgaagcc gcgctcgcgc aagccggtat
      181 cggtattgat cgccaagctg gtaccgcgcg aggcggcctg cgccgtctcg taggcgcgca
      241 gcaggtcgct ggcgtagagc gcggcgacgt cttcttcggc caaggcgagc gcgaggcgat
      301 gcgcctgcca gcggccggtg tcgttgagcg ggatgtcgag ctggccctgg atgcgcgcct
      361 ccacattcca ggccgtctcg ccatggcgga tcgcgatgat gcgcgtcggc tcggggtggg
      421 attcgggatg cgcgctgctc gcggaatcgc ttgggctgtc gggcatgggt cggtcggtgc
      481 agctggtctc tcagccgagc agtcgtttgt tgaggctgta aatcccgctg aaactggctt
      541 cggccagcac atggccgcgc atcgcttcgc gcacctgcgg tccggtgaac tcgccttcca
      601 cggccaggcg ctgcaggtcc agcgcgtaga gcgtgaacac gtagtggtgc agcagcgtgt
      661 cgttgaaggg cgggaagggg ccgtcgtagc cgtaatactg gccgcccatc tgcgcgtcgc
      721 cggcgaacca ggcggtgtag tcgttgatgc cgtggcgcgc gccgtgcaac gtggccgggc
      781 cgctcttgcc gcgcgctgtg aagccgcggc tgaattcgct ctcggcgatc tcgcgcagcg
      841 cgggcggcag atccaccatc acccagtgga agaagtccac gcgcggcagg ctggccggca
      901 cttcgcggtc gggcttgttg acgtcgtcgc cgcggctggg cacgtcgggg tcgtggcaga
      961 tcagcgcgaa gctcatcgtg ccggcgggca catcgttcca ggcgagatgc gggttgaggt
     1021 tgtcggagaa gccgacgcca ccctgggcgt cgtgcttgcc ggcggcgtat tgggcaggga
     1081 tgcgctggcc atcggtccag ctgctgctgg tgagcttcat tgctgttgcc tccttgttct
     1141 atcgagaaga ttcagacgcc ccatacgatg ggttcgtcgg cggccaatgc cagcttcatc
     1201 atctcgacca tgggccaggc gcgctggcgc aggctgatca cgccctccca atggggcggc
     1261 ggcttgccag cggcaagtgc ttcggcttcg gccttggcgc gcaccgcctc gtcggccgcg
     1321 atggcgtctt tgatcgcctg catcgccgcc ggcatggcct gcggctcgat gatgcccttg
     1381 gcggcgggct gcttgccgat gatgccgagc agctgggcac cgctcgcgcc cagcatgatc
     1441 aggtcgcctg cggccttgga cttgaatttg tagagcatcg cgccgtttcg gtcagctgta
     1501 gatgtagccg cgattgaagg gataggccga ttgtgcgccg cgcagcgcct cgccatcggt
     1561 cactacgccg ctgggccgcg aggccagcat ctggtgcagc gcgacccagc cctgctcgaa
     1621 gctcatcgcg ctgccggcga ggtagagccg gtaggcgcgc accacgctct cgcgggtgag
     1681 ctggcgggcg cggtcgagct gggcctccag ggcgtcgctc caggcccaca gcgtgcgggc
     1741 gtagtgcggg cgcaggttct cgacgtccag cgcctccagg ccggcctcgg ccatcgcgcg
     1801 catcacctcg gacacatgca ccagctcgcc gcccgggaag atgtagcgct ccatgaagtc
     1861 gcccatgccg gcgccgagct ggctgttgtg caggccgccc gccgtgatgc cgtggttcat
     1921 cagcagaccg ccgggcttga gcaggctgcg gatcttctcg aaatagccga ccagcctggc
     1981 gcgccccacg tgctcgaaca tgccgaccga ggcgatgcgg tcgtagggct cgtcttcgcc
     2041 aatgtcgcga tagtcgcaca gctccatgcg cacgcggccc tgcaggccct tctcctcgat
     2101 caggcggttg acatgggcgt gctgattctt ggacagcgtg acgccatgcg cctccacgcc
     2161 atagtgctcg gccgcccaca gcagcaggcc gccccagcca gcgccgatgt ccaggaagcg
     2221 catgcccggc tccagcatca gcttgcggca gatatggtcc agcttggcct cctgggcctg
     2281 cgccaggctc atggtggggt cgcggtaata ggcacaggaa tagacgcggc gcgggtccag
     2341 ccacagcgcg tagaagtcgt cggagacgtc gtagtggaac tggatctgct gggcatcgcg
     2401 ctcgcgcgtg tggcgcgcca gcgacttgcc c
//
LOCUS       DASUCY010000533         2327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1232991, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000533 DASUCY010000000
VERSION     DASUCY010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232991"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1214)
                     /locus_tag="VFV25_10495"
     CDS             complement(<1..1214)
                     /locus_tag="VFV25_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1393797.1"
                     /translation="MGIQKPRLSLWQVLNMNVGFFGIQFSFGLQQSSMSPIYKYLGAD
                     EASLPLLWLAGPVTGLVVQPIVGAMSDRTVSRWGRRTPYFLVGALLCSLGLLVMPFSP
                     ALWLAASLLWILDAANNVTMEPYRAYVSDRLDVSQHSIGFLTQSAFTGLAQTLAYLAP
                     SIMVWLGMNKDAVNTHNVPHVTIAAFLIGAVFSITTVWWSTRAVPELPLSRAEEAALR
                     ARPGGWTAALLEVWQAIKEMPPTMRRLWWMKLFQWYGMFCYWMYIVPALASAMYGTHD
                     AASEGFRDAGLLNGRIGGFYNAVACLAALAMVPFTRKFGAKWVHAACLCAAGLGMWCI
                     PGIREQGWLFVPMLGIGLAWASMMGNPYVLLAGSIPPQRAGVYMGIFNMFIVIPMILE
                     SLTLPLIYHHLLG"
     gene            complement(1222..1902)
                     /locus_tag="VFV25_10500"
     CDS             complement(1222..1902)
                     /locus_tag="VFV25_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEY1393798.1"
                     /translation="MSTLAQNPMVLAGPGRGKAFKPKSRFGPVGIVVMVGFHLLLGYA
                     LMSGLARKAVQIVKRPLDATIIEEVKLPPPPPPPPPPRKIVTETPKVDAPPPPAYVPP
                     PEVTPPATAQPAITAVQNTEALAPPLATPAMPIAPVPAGPIRQDIAVACQRQVKPVIP
                     QHALDEGIGGTVVAEIRIRGGRVAEVQITSGPRIYHAAVKAAVMQYQCTSDANDIVAT
                     QTFEFKVD"
     gene            complement(1899..2318)
                     /locus_tag="VFV25_10505"
     CDS             complement(1899..2318)
                     /locus_tag="VFV25_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY1393799.1"
                     /translation="MKRRESTSAGEPVVDINTTPLIDVMLVLLIMLIITIPVQLHAVK
                     LNMPAGNPPQSDVKPEVLRIDIDPRSVVLWNGEPVADRADLEARMERAAALPAQPEVH
                     LRPDRHARYDVVAGVMASAQRIGLSKIGIVGSEQFVQ"
BASE COUNT          402 a          747 c          792 g          386 t
ORIGIN      
        1 cccagcaggt ggtgatagat caggggcagg gtcaggctct cgaggatcat cgggatcacg
       61 atgaacatgt tgaagatgcc catgtagacg cccgcgcgct gcggcggaat gctgccggcc
      121 agcagcacat aagggttacc catcatgctg gcccaggcca ggcctatgcc cagcataggc
      181 acgaacagcc agccttgttc ccgtatgccc gggatgcacc acatgcccag gcccgctgcg
      241 cacaggcagg ccgcatgcac ccacttggcg ccgaacttgc gcgtgaaggg caccattgcg
      301 agcgcggcca ggcaggccac tgcgttgtag aagcctccga tgcggccatt gagcaggcca
      361 gcatcacgga agccctccga ggctgcgtcg tgcgtgccgt acatcgcact ggccagcgcc
      421 gggacgatgt acatccagta gcagaacatg ccgtaccact ggaagagctt catccaccac
      481 agccgtcgca tcgtcggcgg catctccttg atcgcctgcc agacctccag cagcgcggcc
      541 gtccagccgc cgggcctggc gcgcagcgcg gcctcctcgg cccgagacag cggcagctcc
      601 ggcacggcgc gcgtgctcca ccacacggtg gtgatcgaga acaccgcgcc gatcaggaag
      661 gccgcgatcg tgacgtgcgg gacgttgtgg gtgttcacgg catccttgtt catgcccagc
      721 cacaccatga tcgacggcgc aagataggcc agcgtctgcg ccaggccggt gaaggcgctt
      781 tgcgtcagga agccgatcga gtgctgggac acgtcgaggc ggtcgctgac ataggcgcga
      841 tagggctcca tcgtcacgtt gttggccgcg tcgaggatcc acagcagcga ggccgccagc
      901 cacagggccg ggctgaaggg catcaccagc aggcccaggc tgcacagcag cgctccgacc
      961 aggaagtagg gcgtgcggcg cccccagcgc gagaccgtgc ggtcgctcat cgcgccgacg
     1021 atgggctgca cgaccagacc cgtcaccggg ccggcgagcc acagcagcgg caggctcgct
     1081 tcatcggcgc ccagatattt gtagatcggg ctcatgctgc tttgctgcag gccgaagctg
     1141 aactggatgc cgaagaagcc gacattcatg ttcagcacct gccacagcga tagacggggt
     1201 ttctggatac ccatggacgg cttaatcgac tttgaattcg aaggtctgcg tcgccacgat
     1261 gtcgttggcg tcgctcgtgc attggtattg catcactgcc gccttgaccg ccgcgtggta
     1321 gatgcgcgga ccggacgtga tctgaacctc ggcgacgcgg ccaccgcgga tgcggatctc
     1381 ggccacgacg gtgccgccga tgccctcgtc gagagcgtgc tgcggaatca ccggtttgac
     1441 ctgcctctga caggcaacag caatatcctg gcggatcggg ccggcgggga caggcgcgat
     1501 tggcattgcg ggtgttgcta gcggcggggc cagcgcttcg gtgttctgca ctgcggtgat
     1561 ggcaggctgc gcggtcgcag gcggcgtcac ttcgggcggt ggcacgtagg ctggtggagg
     1621 cggcgcgtcg accttgggcg tttcggtaac gatcttcctt ggcggtggag gaggtggcgg
     1681 aggcggcggc agcttgacct cctcgatgat ggtggcgtcc agcggtcgct tgacgatctg
     1741 caccgccttg cgcgccagcc ccgacatcag ggcatagccg agcagcagat ggaagccgac
     1801 catcacgacg atgccgacag ggccgaagcg gctcttcggc ttgaaggcct tgccgcggcc
     1861 ggggccggcc aggaccatgg ggttttgcgc gagagtgctc attggacgaa ctgctccgag
     1921 ccgacaatgc cgatcttgct caggccgatg cgctgcgccg aggccatcac gccggccacc
     1981 acgtcgtagc gcgcatgccg atcggggcgc agatgcacct cgggctgcgc gggcagcgcg
     2041 gccgcgcgct ccatgcgcgc ctcgagatcg gcgcgatccg ccaccggctc gccgttccac
     2101 agcacgacgc tgcgcgggtc gatgtcgatg cgcagcacct caggcttgac gtcgctttga
     2161 ggcggattgc cggcgggcat gttgagcttg accgcgtgca gctgcaccgg gatcgtgatg
     2221 atcagcatga tcagcagcac cagcatcaca tcgatcagcg gcgtggtgtt gatgtccacc
     2281 accggttcgc cggccgaggt gctttcgcgc cgcttcagat atttgtg
//
LOCUS       DASUCY010000534         5360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_815187, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000534 DASUCY010000000
VERSION     DASUCY010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 5360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5360
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815187"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..141
                     /locus_tag="VFV25_10510"
     CDS             <1..141
                     /locus_tag="VFV25_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HEY1393800.1"
                     /translation="PSAFRLEPPGFLLHYWQQGSFVTHQAVIGDYPGPYPFFSPAGEL
                     ID"
     gene            complement(158..733)
                     /locus_tag="VFV25_10515"
     CDS             complement(158..733)
                     /locus_tag="VFV25_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018439175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEY1393801.1"
                     /translation="MDEPNEQRVAAHLARNLISLRHARALTQDALANAAALPRSTIAN
                     LESGDANPSLAVLLKVAAALGVPIDELLAAPRAKVRHWPAADLGSRQRGRGVTERPLV
                     PEPVPDELSSVMDFAPGAVMGGTPHLPGTREFFTCLDGRVTLFVAGDRHELATGDVLA
                     FPGNLPHSYQNPDAERPARGISLVVLAKAGV"
     gene            880..1638
                     /locus_tag="VFV25_10520"
     CDS             880..1638
                     /locus_tag="VFV25_10520"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY1393802.1"
                     /translation="MTTATKQEYRSAPPAAGGHADNGLRIAVTGGTSGLGLALLREWH
                     RRGARLAFVARHAERVAEVARELPGSHGIVGDVANKDDIHRIALQITGRLGGLDVLVN
                     NASSLGPVPLAPLADTDCEDLELALATNLLGPFRLTKALLGALAASARQGRGAVVLNI
                     SSDAALNAYPGWGAYGASKAALHHLSRIWHEELRGTGVQVLALDPGDMDTPLHALAIP
                     DADPASLKRPEQAARELSEAITQALAQATAGAHA"
     gene            1635..2756
                     /locus_tag="VFV25_10525"
     CDS             1635..2756
                     /locus_tag="VFV25_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018439173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HEY1393803.1"
                     /translation="MMIAADRPIQRPTGARLLALDAQGRINQLPRLRLVAELLRRGDL
                     VVANDAATLPASLAGVHLRSGAAIEVRLAGRASLAVDDIRCFSAVVFGEGDWRTLTEE
                     RPLPPPLLPGDVLQLGPLQARIERTLGHPRLVALRLAGTPDGIWAGIARHGRPIQYAH
                     LREPLALWDVWTPIAGEPVAFEPPSAGFVLDWQTLDEMRERGIGFATLTHAAGISSTG
                     DAALDARLPFDEPYRIPAATAQALAATRAQGGRVIAIGTTVVRALEHAASRDGRVHAG
                     EGLADQRITAGTRLRAADAIVSGTHEPGASHYELLRAFAREADLRRADAMLQAGGYRT
                     HEFGDSVWIERARTSASGTAARTRHRQAAVAAPALLRAV"
     gene            complement(2778..3950)
                     /locus_tag="VFV25_10530"
     CDS             complement(2778..3950)
                     /locus_tag="VFV25_10530"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEY1393804.1"
                     /translation="MPNPVIIEALRTPFARRNGAYAGTRPDTLLAHVLNGLLERCGAS
                     AAQIDDVVTGCVSQAGEQGANIGRQAVLLAGWPVQVPAVTLNRMCGSAQQAVHFAAQA
                     VAAGDARYAVGCGVEHMTRVPMFTDLTLGRGDYQGFGTLNPALLQRHALIHQVESAER
                     MAERWGLTRAELDAYAKESHRRAHVAGALQAELLPTPGIDAEGQAVTLAHDEGVRASI
                     DDARMAALQPAMRAPGQGVVTAGNASQISDGAAAVLVADEAAARADGLKPKARFLARV
                     VVGSDPVMQLDGVIPATQKALQRAGLALADIDWIEINEAFASVVLAWAREFKPDMARV
                     NPWGGAIAHGHPLGATGAGLMAKMLAGLAHTGGEFGLQVMCIGHGMATATVIQRLG"
     gene            complement(4029..4361)
                     /locus_tag="VFV25_10535"
     CDS             complement(4029..4361)
                     /locus_tag="VFV25_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393805.1"
                     /translation="MPSLVTDADRYDELQPWFVARLIADTKHHLEQAGLSGEELKSAC
                     ASVVFSFCTLLDNSASFEVDNEEYVPYLVFSKDENSMFYPGVNSYLHEYVYGVLDEVF
                     AEQGGSKG"
     gene            complement(4479..>5360)
                     /locus_tag="VFV25_10540"
     CDS             complement(4479..>5360)
                     /locus_tag="VFV25_10540"
                     /inference="COORDINATES: protein motif:HMM:NF016847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY1393806.1"
                     /translation="DPKHLGVVPGIVMALHTWGRTLNHHPHVHCLVSAGGLAPGDRWR
                     DARKGFLLPVEVVKRLFRGKLLHALSTHLRGHQLRLPPEQPLAHWMGCIRAQWRKNFN
                     VRLAEPYPYGDGVVRYLARYVKGGPINERSLRTVDAQQVAFTYTDHRDGQRKLMRLQP
                     GEFIDRVLWHAPPRGQHTVRHYGLYASGSGHLRALCAKQIAGVRSPSLPPLFSAPSCI
                     TLQPQCPTCHVALNVVDTLQPVHRTGEISSCTSPSPRLCPTPRSIGHATRAFNPTSHV
                     ATHRALFFGLACRLARR"
BASE COUNT          844 a         1891 c         1835 g          790 t
ORIGIN      
        1 cccagcgcgt tcaggctgga gccgccgggc ttcctgctgc attactggca gcagggcagc
       61 ttcgtgacgc atcaggccgt gataggcgac taccccgggc cctatccgtt cttctcgcct
      121 gccggagagc tgatcgactg agcagtaagg agcaccccta cacccccgcc ttcgccagca
      181 ccacgagcga gattccgcgc gccggccgct ccgcatcggg gttctgatag gaatgcggca
      241 ggttgcccgg gaaggccagc acgtcgccgg tcgccagctc gtggcggtcg ccggccacga
      301 agagggtgac gcggccgtcg aggcaggtga agaactcgcg cgtgccgggc agatgaggcg
      361 tgccgcccat cacggcgccg ggggcgaagt ccatcacgct ggagagctcg tcgggcaccg
      421 gctcgggcac cagcggccgc tcggtgacgc cgcgcccgcg ctggcgcgag cccaggtccg
      481 cggccggcca gtggcgcacc ttggcgcgcg gcgcggccag cagctcgtcg atcggcacgc
      541 ccagggccgc agccaccttc agcagcacgg ccagcgaagg attggcgtcg ccggactcca
      601 ggttggcgat cgtcgagcgc ggcagcgccg cggcgttggc caaggcgtcc tgcgtcagcg
      661 cacgggcatg gcgcaggctg atgagattgc gcgccaggtg tgcggcgacg cgttgttcgt
      721 tgggctcgtc cacggttcga cccataggag attggcaagg cgccacagag cgcatgtcca
      781 ccgcatcgac tatccgccaa gcggctggtc aggggcctta gcgcagcggc tgttcgtccc
      841 tactctgtgg ttcacatgaa cgaacacacg gagcaagaca tgacaacagc caccaagcaa
      901 gaatacagga gcgccccgcc agccgcaggc ggacatgcgg ataacggctt gcgcatcgcc
      961 gtcaccggcg gcacctcggg cctgggcctg gccctgctgc gcgaatggca tcggcgcggc
     1021 gcgcgcctgg ccttcgtggc gcgccacgcc gagcgcgtgg ccgaggtggc gcgcgagctg
     1081 ccgggcagcc acggcatcgt cggcgacgtg gcgaacaagg acgacatcca ccgcatcgcg
     1141 ctgcagatca ccggccgcct gggcgggttg gacgtgctcg tcaacaacgc gtccagcctg
     1201 gggccggtgc cgctcgcgcc actagccgac accgattgcg aggacctgga gctggcgctg
     1261 gccaccaatc tgctcgggcc cttccgcctc accaaggcgc tgctcggcgc gctggcggct
     1321 tcggcgcggc agggacgcgg cgccgtcgtg ctcaacatct ccagcgacgc ggcgctcaac
     1381 gcctaccctg gctggggcgc ctatggcgcg agcaaggccg cgctgcatca cctgagccgc
     1441 atctggcacg aggagctgcg cggcaccggc gtgcaggtgc tggccctgga ccccggcgac
     1501 atggacacgc ccttgcatgc gctggcgatc cccgatgccg atccggcgag cctgaagcgc
     1561 ccggagcagg cggcacgcga gctgtcggaa gcgatcacgc aggctcttgc tcaagccacc
     1621 gcaggagccc acgcatgatg atcgccgccg accgccccat ccaacggccg accggagcgc
     1681 gcctgctggc cctggacgcg cagggccgca tcaaccagct gccgcgcctg cgcttggtcg
     1741 ccgagctgct gcgccgcggc gatctggtcg tcgccaacga cgcagctacg ctgccggcca
     1801 gcctcgcggg cgtgcatctg cgcagcggcg ccgcgatcga ggtccggctg gccggccgcg
     1861 cgtcgctggc ggtggacgac atccggtgct tctcggccgt ggtcttcggc gaaggcgatt
     1921 ggcgcactct caccgaagag cggccgctgc ccccgcccct gctgccaggc gatgtgctcc
     1981 agctcggccc gctacaggcg cgcatcgagc gcaccctcgg ccacccgcgc ctggtggcgc
     2041 tgcgccttgc cggcacgccg gacgggatct gggccggcat cgcgcgacac gggcggccga
     2101 ttcaatacgc tcatctgcgc gagcccttgg ccttgtggga cgtgtggacg ccgatcgcgg
     2161 gcgaaccggt cgccttcgag ccgccctcgg ccggcttcgt gctcgactgg cagacgctgg
     2221 acgagatgcg cgagcgcggc atcggcttcg cgacactcac gcatgcggca ggcatctcgt
     2281 ccaccggtga cgcggcgctc gacgcgcgcc tgcccttcga cgagccctat cgcatcccgg
     2341 ccgcgactgc gcaggccctc gctgccacgc gcgcgcaagg cggccgcgtc atcgcgatcg
     2401 gcaccaccgt ggtccgcgcg ctggagcacg cggcctcgcg cgatgggcgg gtgcacgccg
     2461 gcgaaggcct cgccgatcag cgcatcacgg ccggcacgag gctgcgcgcg gcggatgcca
     2521 tcgtctcggg cacgcacgag cccggcgcca gccactacga gctgctgcgc gccttcgccc
     2581 gcgaggccga cctgcgccgc gccgacgcca tgctgcaggc cggcggctac cgcacccacg
     2641 agttcggcga ttcggtgtgg atagagcgag ctcggaccag cgcttccggc actgctgcgc
     2701 gcaccaggca taggcaagca gccgttgcgg cacccgcttt gctgcgtgcg gtgtagcaaa
     2761 gtggaaggct gtcgcgatca gcccaagcgt tggatcaccg ttgcggtagc catgccatgg
     2821 ccgatgcaca tgacctgcag cccgaactcg ccgccggtgt gcgcgaggcc agcgagcatc
     2881 ttggccatca gccccgcgcc ggtggcgccc agggggtgac catgcgcgat ggcgccgccc
     2941 caggggttga cgcgcgccat gtcaggcttg aactcgcgcg cccaggccag caccacgctc
     3001 gcaaaggcct cgttgatctc gatccagtcg atgtcggcca gcgccaggcc ggcgcgctgc
     3061 agggccttct gcgtcgcggg gatcacgccg tcgagctgca tcaccggatc ggagccgacc
     3121 accacgcgcg ccaggaagcg tgccttgggc ttgaggccat ccgcacgcgc ggccgcctcg
     3181 tcggccacca gcacggccgc agcgccgtcg gagatctggc tcgcattgcc ggccgtcacc
     3241 acgccctgcc ccggcgcgcg catcgcaggc tgcagcgcgg ccatgcgcgc atcgtcgatg
     3301 ctggcgcgca cgccctcgtc atgggccagc gtcaccgcct ggccttcggc atcgatgccc
     3361 ggcgtgggca gcagctcggc ctgcagcgcg cccgccacgt gggcgcggcg gtggctctcc
     3421 ttcgcatagg cgtctagctc ggcgcgcgtg aggccccagc gctcggccat gcgctcggcg
     3481 ctctccacct ggtggatcag cgcatggcgc tgcagcagcg ccgggttcag cgtgccgaag
     3541 ccctggtagt cgccgcggcc cagcgtgagg tcggtgaaca tcggcacccg cgtcatgtgc
     3601 tccacgccgc agcccaccgc ataacgcgca tcgcccgcgg ccaccgcctg cgccgcaaag
     3661 tgcaccgcct gctgcgccga gccgcacatg cgattgagcg tgacggccgg cacctgcacc
     3721 ggccagcccg ccagcagcac cgcctgacgg ccgatgttgg cgccctgctc gccggcctgg
     3781 ctcacgcaac cggtcaccac gtcgtcgatc tgcgctgcgc tcgcgccgca gcgctccaac
     3841 aggccgttca gcacatgggc cagcagcgtg tcgggccgcg tgccggcata ggcgccgttg
     3901 cggcgggcga agggcgtgcg cagggcttcg atgatgacgg ggttgggcat ggcggactcc
     3961 ggcgacgttt atggtgcggc ggctgcaatc taggcgcgat cgtgcgcagg tggaagtctc
     4021 ttcctcactc agcccttcga cccaccttgt tcggcgaaaa cttcgtcgag cacgccatac
     4081 acgtactcat gcaggtacga gttgacgcct ggataaaaca tgctgttctc gtctttggaa
     4141 aagacgaggt acgggacgta ctcctcgttg tcgacctcaa agctcgccga gttgtccaag
     4201 agcgtgcaga aggaaaagac gacagacgca catgccgact tgagttcctc gcccgacagc
     4261 ccggcctgct ccaaatgatg cttcgtgtca gcaatcagcc gagcgacaaa ccagggctga
     4321 agctcgtcgt acctgtcggc gtcagtaact agtgagggca cggctgaact cctgcattgg
     4381 tggaagtgac agggcgctgg agtttgccgc gcaccactca gcaactgctc gatatccgtc
     4441 ccgctgccac agccagttgc acgacacgac catgaggcct agcgtctagc taatcggcac
     4501 gccaggccga aaaaaagggc tcggtgtgtg gcgacatggg atgtggggtt gaacgcgcgc
     4561 gtggcgtgtc cgattgagcg aggggttggg caaagccgcg ggctcggtga tgtacaagag
     4621 gaaatctccc cagtacggtg cacgggctgc agtgtgtcca caacgttcaa cgcgacatga
     4681 caggtcgggc attggggctg cagtgtgatg caagacggcg cgctgaacaa tggaggcaac
     4741 gaggggctgc gcacgcccgc gatctgtttg gcacacaggg ctcgcagatg ccctgagccg
     4801 ctcgcgtaca ggccgtagtg gcgcaccgtg tgctgccccc tgggtggcgc gtgccacagg
     4861 acgcggtcga tgaactcgcc gggctgcagc cgcatgagct tgcgctgccc atcgcgatgg
     4921 tcggtgtagg tgaatgcaac ctgctgggca tcgaccgtac gcaggctgcg ctcattgatc
     4981 ggaccgccct tgacgtagcg ggcaaggtag cgcaccacgc catccccata tggatacggc
     5041 tcggccaggc ggacgttgaa attcttgcgc cactgcgcgc ggatgcagcc catccagtga
     5101 gccagcggct gctcgggtgg taaccgcagc tgatgtccgc gcaggtgggt gctcaacgcg
     5161 tgcaacagct tgccgcgaaa cagccgcttg accacctcca caggcagcaa gaaccccttg
     5221 cgcgcatcac gccaccggtc gcccggtgca agccccccgg ccgataccag acagtgcaca
     5281 tgcggatgat ggttcagcgt gcggccccag gtgtgcagcg ccatcacgat gcctggcacg
     5341 acgcccaggt gcttcgggtc
//
LOCUS       DASUCY010000535         2087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1570289, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000535 DASUCY010000000
VERSION     DASUCY010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1570289"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..990)
                     /locus_tag="VFV25_10545"
     CDS             complement(61..990)
                     /locus_tag="VFV25_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY1393807.1"
                     /translation="MGFFLETLFGGLMAGMLYSLIALGFVLIYKASGVFNFAQGAMVL
                     FAALAMARFAEWIPQWTGIESRLFANVAAFLLAMAVMVAVAWLVEKLCLSKLVNQEGI
                     TLLMATLGITYFLDGFGQTLFGSDIYKIDVGMPKDPVIVAESLFHGGILISKEDLYAA
                     LIAAALVLGLTMFFQKTATGRALRAVADDHQAAQSIGIPLSRIWVIVWSVAGFVALVA
                     GIIWGSKLGVQFSLSLVALKALPVVILGGLTSVPGAIFGGLIIGVGEKLSEIYIGPLF
                     GGGIEIWFAYVLALMFLLVRPQGLFGEKIIDRV"
     gene            complement(1007..1837)
                     /locus_tag="VFV25_10550"
     CDS             complement(1007..1837)
                     /locus_tag="VFV25_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEY1393808.1"
                     /translation="MPFHEAVDAANDAKTTTGRRVGDVVLEVQNISLAFGGVKALTDI
                     SFNVREHEIRAIIGPNGAGKSSMLNCINGVYQPQQGSITFRGRHFKHMNSRQVAEMGV
                     ARTFQNLALFKGMSVLDNIMTGRNLRMKSNLLLQALRFGPAEREEIAHREVVERIIDF
                     LEIQAHRKTPVGRLPYGLQKRVDLGRALAMEPQVLLLDEPMAGMNVEEKQDMCRFILD
                     VNDEFGTTIVLIEHDMGVVMDISDRVVVLDYGKKIGDGRPDEVRGNEDVIRAYLGTSH
                     "
     gene            complement(1867..>2087)
                     /locus_tag="VFV25_10555"
     CDS             complement(1867..>2087)
                     /locus_tag="VFV25_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEY1393809.1"
                     /translation="LDADDGELTRTRKVRRGFIAEKYAVLVDALYGGKGSQYIETQVK
                     FEDGRSGVVSAELSIVDVPTYPAVRRAA"
BASE COUNT          370 a          688 c          664 g          365 t
ORIGIN      
        1 agatctgctg gtcggcgcgg tagcttgcct tgaactggcc gttttcgcgg tagagcatga
       61 tcagacccga tcaatgattt tttcgccgaa caagccttgc ggccgcacca gcaggaacat
      121 tagtgccagc acataggcga accagatttc gatgccgccg ccgaacagcg gcccgatgta
      181 gatctccgag agcttctcgc ccacgccgat gatcaggccg ccgaagatgg cgccgggcac
      241 cgaggtcagg ccgccgagga tcacgaccgg cagcgccttc agcgcgacca gcgacagcga
      301 gaactgcacg cccagcttgc tgccccagat gatgcccgcc accagcgcga cgaagccggc
      361 caccgaccac acgatgaccc agatgcggct caaggggatg ccgatggact gcgcggcctg
      421 gtgatcgtcg gcaacggcgc gcagcgcgcg gccggtggcg gtcttctgga agaacatcgt
      481 gaggccgagc accagcgcgg cggcgatcag cgcggcgtag aggtcttcct tgctgatgag
      541 aatgccgccg tggaacaggc tctcggccac gatcaccggg tccttgggca tgccgacgtc
      601 gatcttgtag atgtcggagc cgaacagcgt ctggccgaag ccgtcgagga agtaggtgat
      661 gcccagcgtc gccatcaaca gcgtgattcc ttcctggttg acgagcttcg agaggcacag
      721 cttctcgacc agccaggcga cggcgaccat cactgccatc gcaagcagga aagccgccac
      781 attggcaaac agcctgctct cgatgccggt ccattgcggg atccattcgg cgaagcgcgc
      841 catcgccagc gcggcgaaca acaccatcgc gccctgggcg aagttgaaga cgccgctggc
      901 cttgtagatc agcacgaagc ccagcgcgat cagcgaatag agcataccgg ccatcaggcc
      961 gccgaacagt gtttcgagga agaagcccat cgcgcgccgt ccttcttcag tggctcgtgc
     1021 ccagataggc gcggatcacg tcctcgttgc cgcgcacttc gtcgggcctg ccgtcgccga
     1081 tcttcttgcc gtagtcgagc acgaccacgc ggtcgctgat gtccatcacc acgcccatgt
     1141 cgtgctcgat cagcacgatg gtggtgccga actcgtcgtt cacgtcgagg atgaagcggc
     1201 acatgtcctg cttttcctcg acattcatgc cggccatggg ctcgtccagc agcagcacct
     1261 ggggctccat cgcgagcgca cggcccaggt ccacgcgctt ttgcaggccg tagggcaggc
     1321 gccccaccgg cgtcttgcga tgcgcctgga tctcgaggaa gtcgatgatg cgctccacca
     1381 cctcgcggtg cgcgatctcc tcgcgctcgg ccggcccgaa gcgcagcgcc tgcagcagca
     1441 ggttgctctt catgcgcaga ttgcgcccgg tcatgatgtt gtccagcacg ctcatgccct
     1501 tgaacagggc taggttctgg aaggtgcgcg ccacgcccat ctcggccacc tggcgcgagt
     1561 tcatgtgctt gaagtgccgg ccgcggaagg tgatggagcc ctgctgcggc tggtagacgc
     1621 cgttgatgca gttcagcatc gagctcttgc ccgcgccgtt ggggccgatg atggcgcgga
     1681 tctcgtgctc gcggacattg aagctgatgt cggtcagcgc cttcacgccg ccgaaggcga
     1741 gcgagatgtt ttgcacctcg aggaccacgt cgccgacgcg gcgaccggtg gtcgttttcg
     1801 cgtcattggc cgcatcgacc gcctcgtgga agggcatagg cgctgcgcgc agggcggcag
     1861 tcgtcatcag gcggccctcc gcacggcggg ataggtcggc acgtcgacga tgctcaactc
     1921 ggccgagacg acaccgctgc gcccgtcctc gaacttcacc tgggtctcga tgtattgcga
     1981 gcccttgccg ccatagagcg catcgaccag cacggcgtac ttctcggcga tgaagccgcg
     2041 cctgaccttg cgcgtgcgcg tcaactcgcc gtcgtcggcg tcgagtt
//
LOCUS       DASUCY010000536         3727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_12430, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000536 DASUCY010000000
VERSION     DASUCY010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12430"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..149
                     /locus_tag="VFV25_10560"
     CDS             <1..149
                     /locus_tag="VFV25_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEY1393810.1"
                     /translation="DVNYVGSFAGAMTQSPSDSSPDEVPSGDLETARLFGKRVAEVAA
                     KLVN"
     gene            199..801
                     /locus_tag="VFV25_10565"
     CDS             199..801
                     /locus_tag="VFV25_10565"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEY1393811.1"
                     /translation="MNNDKPLRVLVAAGSSRHGAYSGQLARAAARIAEAGGAAVTRID
                     LRSLELPVYDGDLEAASGVPAGGYRLRDLFASHDALLFVTPEYNGFPTPLFINAMDWL
                     SRVPAQGDAPAGLAATAGKAAGLLSSSPGPLGGLRALNYTRQYLSMAFGMLVVPQQFA
                     LGQAHNAFDEQGVLKDAKHQQAVENVVRSLLKVGVALNRE"
     gene            823..1236
                     /locus_tag="VFV25_10570"
     CDS             823..1236
                     /locus_tag="VFV25_10570"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY1393812.1"
                     /translation="MTIQAQLVRNQPAPFGSLAQLSDGRHAWLADVPTDLGGGDLGAD
                     PHDLLDSALAGCTLLTLELYIRRKQLAVSALRVQIEHEETKAADGRVRYQLRRQLHIE
                     GELSAEERQRLLEIANKCPIHRVLTGEISVGTSLA"
     gene            complement(1332..2081)
                     /locus_tag="VFV25_10575"
     CDS             complement(1332..2081)
                     /locus_tag="VFV25_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEY1393813.1"
                     /translation="MPETLNIAAYKFARINERESLQASLRSHASAAGLKGTVLLAEEG
                     INLFLAGEAEPLRGFLRWLRGDARFADLEAKESWSAGQPFKRLLVKLKREIIRMDHPA
                     ITPTSGPASGRAPAVNAATLARWLAAGRDDAGREVVMLDTRNGFEVDVGAFEGAIDWR
                     LRKFSDFPQALLANREALKDKTVVSYCTGGIRCEKAAIFMREAGLEHALQLDGGILKY
                     FETVGARHWRGACFVFDERTALDPALKPVGA"
     gene            2248..3276
                     /locus_tag="VFV25_10580"
     CDS             2248..3276
                     /locus_tag="VFV25_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY1393814.1"
                     /translation="MFTRWWPRASPPALNLALQGGGAHGAFTWGVLEALLDEPRIRFE
                     GLSGSSAGAMNAVVLADGWMKGGREGARRGLEDFWTAVGRQLPAAAAQGEGEEIGLSA
                     AAKLFAKWAGNFAPSQLNPFDLNPLRDLLRRQVDFESLRKHSPFRLFIGTTRANTGKL
                     RLFREHELTLEALLASACLPTVHHTVEIDGEPYWDGGYCANPAVFPLFYDCKARDVLL
                     VLLNPLVREGTPRSRLEIEARIVELGFNVHFMREMRMFVMATEFANASFLTRGRLERR
                     LQQMRFHMVDSCDVASLQRNETKLLAYGPFLELLRGQGRERGEAWVAAHAGDVGRRSS
                     IDLKQWIA"
BASE COUNT          628 a         1246 c         1250 g          603 t
ORIGIN      
        1 acgacgtcaa ctatgtcggc tccttcgccg gcgcgatgac gcagtcgccc tcggactcca
       61 gccccgacga agtgccgtcc ggcgacctgg aaaccgcccg cctcttcggc aagcgcgttg
      121 ccgaggtggc ggccaagctg gtcaactgat tgaccaactg atcgcctcgc gctgcgggct
      181 acacgagagc gagcttccat gaacaacgac aagccattgc gagtgctggt cgcggcaggg
      241 agctcgcgcc atggggccta tagcggccag ctggcccggg ctgccgcgcg cattgccgag
      301 gcgggcggcg cggccgtcac gcgcatcgat ctccgcagcc tggagctgcc cgtctacgac
      361 ggcgacctcg aggcggccag cggcgtgcct gcgggcggct acaggctgcg cgatctcttc
      421 gccagccacg acgccttgct cttcgtcacg ccggaataca acggcttccc gacgccgctc
      481 ttcatcaatg cgatggactg gctctcgcgc gtgcccgcgc agggcgatgc gccggccggc
      541 ctcgcggcca cggcgggcaa ggcggcgggg ctcctgagca gctcgcccgg cccgctgggc
      601 ggcctgcgtg cgctcaacta cacgcgccaa tacctgtcga tggccttcgg catgctggtc
      661 gtgcctcagc agttcgcgct cggccaggcc cacaatgcct tcgacgagca aggcgtcctg
      721 aaggacgcca agcatcagca agcggtcgag aatgtggtgc gctcgctgct caaggtgggc
      781 gtcgcgctga accgcgaata acttcctctc ggagcccagc ccatgaccat ccaagcccaa
      841 ctcgtgcgca atcagccggc gcctttcggc tccctggccc agctcagcga tggccgccac
      901 gcctggctgg ccgatgtacc gaccgacctg ggcggcggcg atctcggcgc cgacccgcac
      961 gatcttctcg attccgcgct ggccggctgc accctgctca cgctggagct ctacatccgc
     1021 cgcaagcagc tcgccgtgag cgccttgcgc gtgcagatcg agcatgagga aaccaaggcc
     1081 gccgacggcc gcgtgcgcta ccagctgcgc cggcagctgc acatcgaggg cgagctgagc
     1141 gctgaagagc gccagcggct gctggagatc gccaacaaat gcccgatcca tcgggtgctg
     1201 accggtgaga tcagcgtggg gacgagcctg gcgtgaaagc ttgcgccgag tgctactgaa
     1261 tatgtttcag tagtcgatgg aattccacca gtaatcggcg gaaaagtcgt cgaaaccctg
     1321 ccgaagctcg atcaggcgcc gaccggctta agcgccggat ccagcgccgt gcgttcatcg
     1381 aagacgaaac aggcgccgcg ccagtgccgg gcgcccacgg tctcgaaata cttgaggatg
     1441 ccgccgtcga gctgcagcgc atgctcgagg ccggcttcgc gcatgaagat cgcggccttc
     1501 tcgcagcgta tgccgccggt gcaatagctc accaccgtct tgtccttcag cgcctcgcga
     1561 ttcgcgagca gggcctgcgg aaagtcgctg aacttgcgca gacgccagtc gatcgcgccc
     1621 tcgaaggcgc cgacatcgac ctcgaagccg ttgcgcgtgt ccagcatcac cacctcgcgg
     1681 ccggcatcgt cgcggcccgc ggccagccag cgcgccagcg tcgccgcatt gacggccggc
     1741 gcgcgtcccg atgcggggcc gcttgtggga gtgatggcgg ggtgatccat gcggatgatc
     1801 tcgcgcttga gcttgacgag caggcgcttg aagggctggc cggccgacca gctctccttg
     1861 gcttccaggt ccgcgaagcg cgcgtcgccg cgcagccagc gcaggaagcc gcgcagcggc
     1921 tctgcctcgc cggcgaggaa gagattgatg ccctcctcgg ccagcagcac cgtgcccttc
     1981 aggcccgcgg cactcgcatg tgagcgcagg cttgcctgca ggctctcgcg ctcgttgatc
     2041 cgggcgaact tgtaggcggc gatgttgagc gtctcgggca tccctgcatt ttagaaatgg
     2101 aaagccgtgg caaggcagac aggcatacgc attgctaccg tgatcgagcg actgccatgg
     2161 catagtgatc tgttgcttta gcgaaggaga gcagtggtca acagcagtgc aagtgaaaag
     2221 ccggcggaat cgcaagcagg gcgaggcgtg ttcacacgct ggtggccgcg cgcatcgccg
     2281 cccgcgctca acctcgcgct gcaaggcggc ggcgcccacg gcgccttcac ctggggcgtg
     2341 ctggaggccc tgctcgacga gccgcgcatc cgcttcgaag gcctgagcgg cagcagcgcc
     2401 ggggcgatga atgcggtggt tctcgccgac ggctggatga agggcggacg cgaaggggcg
     2461 cgccggggcc tggaagattt ctggaccgcg gtcggcaggc agctgccagc ggccgctgcc
     2521 cagggcgagg gcgaggagat cgggctgtcg gcggccgcca agctgttcgc gaaatgggcc
     2581 ggcaatttcg cgccgtccca gctcaatccc ttcgatctga acccattgcg tgacctgctg
     2641 cgccgacagg tcgacttcga gtctctgcgc aagcacagcc ccttcaggct cttcatcggc
     2701 accacgcggg ccaacaccgg caagctgcgc ctgttccgcg agcacgagct cacgctggag
     2761 gcgctgctcg cctcggcctg cctgcccacc gtccaccaca cggtggaaat cgacggcgag
     2821 ccctattggg acggcggcta ctgcgcgaac ccggcggtgt tcccgctttt ctacgattgc
     2881 aaggcgcgcg acgtgctgct ggtgctgctc aacccgctgg tgcgcgaagg cacgccgcgc
     2941 agccggctcg agatcgaagc gcgcatcgtg gagctgggct tcaacgtcca tttcatgcgc
     3001 gagatgcgca tgttcgtgat ggcaaccgaa ttcgcgaacg cctccttcct gacgcgaggt
     3061 aggctggagc ggcggctgca gcaaatgcgc ttccacatgg tcgattcctg cgatgtcgcg
     3121 agcttgcagc gcaacgagac caagctgctt gcctatgggc cgtttctcga gctgctgcgc
     3181 gggcagggaa gggagcgtgg cgaggcgtgg gtcgcggccc atgctgggga tgtggggcgg
     3241 aggtcgagca tcgacctcaa gcaatggatt gcttgaggac cttggttagg ggctttgtag
     3301 ttgctttgcc ttcgtgcggg gcaccccgcc gggattccgg cccggcagcc gggtaacttt
     3361 ggtgtccaca tcacttcgct gcgcgaaatg agtggccccc ggcttcgcgc actcttgcgt
     3421 gcccaagaga aagtcaccaa agagaagggc accccaactg tgccggtcgg cctgaacggc
     3481 cgactgctct gcggggctgg cagcccgagg gcgagcaaag aactcgccct tattgcggct
     3541 gcgccgcaaa cggagctcaa acagctttgc tcgaccgcgc tgcgcgcagc aaccctcggg
     3601 ccgctgcgct cctcgacggc ccagccatgg ggccccgtga attcgagtca gatcgacaag
     3661 ccggctaggg ccggcaaggc gttgcgagcg cggcgccccg cacgaaagta aagcaagctg
     3721 caaagtg
//
LOCUS       DASUCY010000537         4452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1104673, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000537 DASUCY010000000
VERSION     DASUCY010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4452
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104673"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1565)
                     /locus_tag="VFV25_10585"
     CDS             complement(<1..1565)
                     /locus_tag="VFV25_10585"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HEY1393815.1"
                     /translation="MNSSYQAFYRQSLDRPDAFWAEQARLIDWHRPYEQVCDYSNPPF
                     ARWFVGGQTNLCHNAVDRHLAGRAEQNALIWVSTEVDQERVYSFAELHAEVQRMAAVL
                     IGLGVERGDRVLIYMPMIPEAVFAMLACARIGAIHSVVFGGFASVSLASRIDNARPKL
                     IVSADAGSRSGKVVAYKPLLDEALRICAHPADKVLLVDRGLAPMERQAGRDLDYALLR
                     ERYLKAQVPCTWVDSDHPSYTLYTSGTTGTPKGVQRDTGGYAVALAASMKHIFCGQAG
                     ETYFSTSDIGWVVGHSYIVYGPLIAGMATVLYEGLPIRPDAAVWWSIVEKYKVTAMFS
                     APTAVRVLKKQDPAALTRHDLSSLRALFLAGEPLDEPTSNWIAQALGKPIIDNYWQTE
                     TGWPILTIANGVERQPSKPGSPGKPMYGYDVKLLDENSGEELHGADQKGVLMIEGPLP
                     PGCLQTVWGDDERFVKTYWSRIAGRWLYSTFDWGIRDADGYYFILGRTDDVINVAGHR
                     LGTREIEESISSHP"
     gene            1890..2516
                     /locus_tag="VFV25_10590"
     CDS             1890..2516
                     /locus_tag="VFV25_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEY1393816.1"
                     /translation="MSCAQAAPDADPVARFLSEKGLVNNSGSNATAAPASEPGLVQKV
                     RDRASDVASDLVLSAMNFLGVPYKRGGQTEEAGFDCSGFTRHIFEASVGLVLPRRSEE
                     QARAPGLLNVARSELKPGDLVFFNTLRHTFSHVGIYLGDNKFIHAPRSGAEVRIEDMR
                     VAYWSQRFNGARRVPQADAPVDAPPASLSAPALPQAGMVNGVAQSATN"
     gene            2584..3702
                     /gene="moaA"
                     /locus_tag="VFV25_10595"
     CDS             2584..3702
                     /gene="moaA"
                     /locus_tag="VFV25_10595"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEY1393817.1"
                     /translation="MAERSIPLVDHRSAGLVPTVPRHLTPPTGLLSDTLGRPLHDLRI
                     SVTDRCNFRCGYCMPKDVFGHDHAFLPHKDLLSFEEITRLAGIFVAHGVHKLRLTGGE
                     PLLRKDLEKLVAMLAGLRTPAGEPLDLTLTTNASLLARKADALKAAGLKRVTVSLDAL
                     DDALFRRMNDMDFPVAEVLAGIDAALAAGLAPLKVNMVVQRGINDHEILPMARHFREH
                     YAHRVVLRFIEFMDVGSTNGWRMDEVLPSAEVLQRIAAVYPIQPMEATAPGETAQRWR
                     YADGGGEIGVISSVTQAFCRDCNRARLSTEGKLYLCLFAGRGHDLRALLRQGASDAEI
                     AAAIGLIWHRRDDRYSELRGSAEASERSAKVEMSYIGG"
     gene            3704..4288
                     /gene="mobA"
                     /locus_tag="VFV25_10600"
     CDS             3704..4288
                     /gene="mobA"
                     /locus_tag="VFV25_10600"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HEY1393818.1"
                     /translation="MPRPSITGLVLCGGRGSRMGGVDKGLQPLAGKPLVEHALQRLRP
                     QVGALAVNANRHLEIYAGLGLPVWPDALAGYPGPLAGILAGLEACTTPWLMTVPCDAP
                     CFPLDLVARLADAAEKAAADIAVPLADGRTQPVFCLAKASLAGGLREFMRGEERRVEA
                     WLDRHPLVRLPFDDAAAFANVNTHQDLMSLEGTA"
BASE COUNT          691 a         1527 c         1494 g          740 t
ORIGIN      
        1 ggatggctgg aaatgctctc ctcgatctcg cgcgtgccca ggcggtggcc ggcgacgttg
       61 atcacgtcat cggtgcggcc gaggatgaag tagtagccgt cggcatcgcg tataccccag
      121 tcgaaggtgg aatagagcca gcgcccggct atccggctcc aataggtttt cacgaagcgc
      181 tcgtcgtcgc cccacaccgt ctgcagacag ccaggcggca aggggccttc gatcatcagc
      241 acgcctttct ggtcggcgcc atgcaattcc tcgccgctgt tctcgtccag cagcttcacg
      301 tcgtagccgt acatcggctt gcccggcgag ccgggcttgc tgggctggcg ctcgacgccg
      361 ttggcgatgg tgaggatggg ccagccggtc tcggtctgcc agtagttgtc gatgatgggc
      421 ttgcccaggg cctgggcgat ccagttcgag gtgggctcgt ccagcggctc gccggcgagg
      481 aagagcgcgc gcagcgagct caggtcgtgc cgcgtcagtg cggcgggatc ctgcttcttc
      541 agcacgcgca ccgcggtggg ggcactgaac atcgccgtga ccttgtattt ctcgacgatg
      601 gaccaccaga ccgccgcgtc cggccggatc ggcaagccct cgtagagcac ggtcgccatg
      661 ccggcgatca gcggtccgta gacgatgtag ctgtggccga cgacccagcc gatgtcgctg
      721 gtcgagaaat aggtctcgcc cgcctggcca cagaagatgt gcttcatgct cgcggccagc
      781 gccaccgcat agccgccggt gtcgcgctgc acgcccttgg gcgtgccggt ggtgccgctg
      841 gtgtagagcg tgtagctggg gtggtcggag tcgacccagg tgcagggcac ttgggccttc
      901 aggtagcgct cgcgcagcag cgcgtagtcc aggtcgcggc cggcttggcg ctccatgggc
      961 gccaggcccc gatcgaccag cagcaccttg tcggcgggat gggcgcagat gcgcagggcc
     1021 tcgtccagca gcggcttgta ggccacgacc ttgccgctgc gcgagccggc atcggcgctg
     1081 acgatgagct tgggcctggc gttgtcgatg cggctggcca gcgagacgct ggcgaagccg
     1141 ccgaacacca ccgaatggat cgcgccgatg cgcgcgcagg ccagcatcgc gaacaccgcc
     1201 tcgggaatca tcggcatgta gatcagcacg cggtcgccgc gctcgacgcc caggccgatc
     1261 agcacggcgg ccatgcgctg cacttctgcg tgcagctcgg cgaagctgta gacgcgctcc
     1321 tggtcgacct cggtcgagac ccagatcagc gcgttctgct cggcccggcc ggcgagatgg
     1381 cgatccaccg cgttgtggca cagattggtc tggccgccga cgaaccaccg ggcaaacggc
     1441 ggattcgagt agtcgcagac ctgctcataa gggcggtgcc agtcgatcag ccgcgcctgc
     1501 tcggcccaga acgcatcggg cctgtccagg gattgccggt agaaggcctg gtagctgctg
     1561 ttcatcgcgc tgtctcctcg ctctctggct tctctgcgcc ttgggtctta tataagaccg
     1621 gctgccaccc ggattgaagc ccagtccact gactatccgc tgactgggga tctggggcca
     1681 tgcgctgcgt cagtccgacg ccgagggtca tcccctagcg gtttaccgca gaaatcatgt
     1741 cgagctttca cgatgtgatc taagcgcttg taaacaaagg atttttttct tgacgcgcac
     1801 cggggtgccc gactactctt gcggccatga aaaggtcgtt gggtcggtgc cttgccgggc
     1861 gcctcgggtt tcttgtgctg ctggcgggcg tgagctgcgc ccaggcagcc ccggacgccg
     1921 acccggtggc ccgcttcctg agcgaaaagg gcttggtcaa caacagcggc agcaacgcta
     1981 ccgcggcacc ggccagcgaa cccggcctgg tgcagaaggt gcgtgatcgc gctagcgacg
     2041 tcgcctccga tctcgtgctg tcggccatga acttcctggg cgtgccctac aagcgcggcg
     2101 gccaaaccga agaggccggc ttcgactgca gcggcttcac gcgccacatc ttcgaggcca
     2161 gcgtcgggct ggtgctgccg cgccgctccg aggagcaggc ccgcgcaccc gggctgctga
     2221 acgtggcacg cagcgaactc aagcccggcg acctcgtgtt cttcaatacg ctgcgccata
     2281 ccttctcgca cgtgggcatc tacctgggcg acaacaagtt catccatgcg ccgcgcagcg
     2341 gcgccgaggt gcgcatcgag gacatgcgtg tcgcctactg gagccagcgc ttcaacggcg
     2401 cgcggcgcgt cccgcaggcc gatgcgccgg tggacgcccc gcctgcgtcg ttgtccgcac
     2461 cggccctgcc acaggccggc atggtcaacg gcgtcgccca gagcgccaca aactaatcct
     2521 cgccgtcgcg gcggctatcc ctcgcccgcc gccgggactg tgcccgcgag gcacaatcga
     2581 cccatggccg agcgcagtat tcctctagtc gaccaccggt ctgcaggcct ggtgccgacc
     2641 gtgccccggc atttgacccc gccgaccggc ctgctgagcg ataccctcgg gcggcccctg
     2701 catgacctgc gcatctcggt cacagaccgc tgcaacttcc gctgcggtta ttgcatgccc
     2761 aaggacgtgt tcggccacga tcacgccttc ctgccgcaca aggatttgct cagcttcgag
     2821 gagatcacgc ggctggcggg catcttcgtc gcccatggcg tgcacaaact gcgcctcacc
     2881 ggcggcgagc ccctgctgcg caaggacctg gagaagctgg tcgcgatgct ggccgggctg
     2941 cgcacgccgg ccggcgagcc gctggacctg acgctgacga ccaacgcctc gctgctggcc
     3001 cgcaaggccg acgctctcaa agcggcgggt ctcaagcgcg tcaccgtgag cctggacgcg
     3061 ctggacgacg ccctgttccg gcgcatgaac gacatggact ttccggtggc cgaggtgctg
     3121 gccggcatcg atgcggccct ggctgccggt ctcgcgccgc tcaaggtcaa catggtggtc
     3181 cagcgcggca tcaacgacca cgagatcctg ccgatggcac gccacttccg cgagcactac
     3241 gcgcatcgcg tcgtgctgcg cttcatcgaa ttcatggacg tgggcagcac caacggctgg
     3301 cgcatggacg aggtgctgcc ctcggccgaa gtgctgcagc gcattgctgc cgtctatccc
     3361 atccagccca tggaggcgac agcgccgggc gagaccgccc agcgctggcg ctatgccgac
     3421 ggcggcggcg agatcggcgt gatctccagc gtgacccagg ccttctgccg cgattgcaac
     3481 cgcgccaggt tgtccacgga aggcaagctc tatctctgcc tcttcgccgg ccgcggccat
     3541 gatctgcgcg ccctgctgcg ccagggcgcg agcgatgcgg agattgccgc ggcgataggc
     3601 ttgatctggc accggcgcga cgaccgctat tccgagctgc gcggcagcgc cgaggcgagc
     3661 gagcgcagcg ccaaggtgga aatgagctac atcggtggct gaaattccga gaccttcgat
     3721 cactggcctc gtgctgtgcg gcggccgcgg cagtcgcatg ggcggcgtcg acaaggggct
     3781 gcagccgctg gcaggcaagc cgctggtcga gcacgcgctg cagcgcctcc ggccccaggt
     3841 gggcgcgctc gcggtcaacg ccaatcgcca cctcgagatc tatgccgggc tgggcctgcc
     3901 ggtgtggccc gatgccctcg caggctatcc cggcccgctg gcgggcatcc tggccggcct
     3961 ggaagcctgc accacgccct ggctgatgac cgtgccctgc gacgcaccgt gcttcccgct
     4021 cgatttggtg gcgcgcctgg ccgatgcggc agagaaggcc gccgccgaca tcgcggtgcc
     4081 cttggccgac ggacgcaccc agccggtgtt ctgcctcgcg aaggcgagcc tggccggagg
     4141 gctgcgcgaa ttcatgcgag gcgaagagcg ccgcgtcgag gcctggctgg atcggcaccc
     4201 tctggtgcgg ctgcctttcg acgacgcagc ggccttcgcc aacgtcaaca cgcaccaaga
     4261 cttgatgagc ctggagggga cggcgtgaac gacgacatgc gcggcatcgc gatccgccgg
     4321 ttggggccgg ccgacctctc ggcctacaag cagttccgcg atcaggccct gctgcggcac
     4381 ccggatgcct tcacctccga ccacgagacc gagcgtgccc gctcgccgga aagctacctg
     4441 ggccgactgg gc
//
LOCUS       DASUCY010000538         7905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_301549, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000538 DASUCY010000000
VERSION     DASUCY010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 7905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7905
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301549"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VFV25_10605"
     CDS             complement(<1..284)
                     /locus_tag="VFV25_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393819.1"
                     /translation="MKRRDLVGLIACAPAAGMLAWPATAASGSPTALDAAGQMKALPM
                     EAGPEGFAWQRVAEGLQFRVGGLTKSVLFYGPGLVRVTAHQGQAYTTQPSL"
     gene            complement(314..2995)
                     /locus_tag="VFV25_10610"
     CDS             complement(314..2995)
                     /locus_tag="VFV25_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HEY1393820.1"
                     /translation="MNSCLIVASALALSLATCVAAAPLSPSMQRAQALVAQMTLAEKA
                     SQLQHDSPAIPRLGIPAYNWWSEGLHGVARADVATVFPQAIGLAATWDAPLVQRVGDV
                     ISTEFRAKFLNTVGADGSSGLYRGLTVWSPNVNIFRDPRWGRGQETYGEDPYLTSRMG
                     VAFIQGLQGPDANQPRVSAAVKHFAVHSGPEADRHREDVKVSPRDLVDTYLPAFHAAV
                     TEGRAESVMCAYNAVDGVPACANERLLQHYLRDEWKFQGHVVSDCGAVADIYMDWAHH
                     HAKTPEEAVALAVRAGTDLICDFGGNKTAAPATTVAAVQQGLLSEAELDRALHRLFDV
                     RLRLGLLASAGQRPFPEITTHDFDTPAHRALNLESARRSLVLLKNDGVLPLKAPPRRI
                     AVIGPNADSVDALVGNYNGTPSKPITLLAGLKARFPGARIDFVEGTGWVAPPLEDLPA
                     SSLCADAACSRAGLHFERFANTKLEGAPSSAALTPQAVFKWGWPDAYDRKESARWTGF
                     LRAGESGKHLFRLKGNRGYRIWIDGELVVDLWDIAWPTSTRGVMLKAGQTYALRVEAL
                     QTGWDGEQRLQWSRPGASDEAAVAAAREADLVVFASGLTWELEGEEMTVLAPGFAGGD
                     RTQIELPAPQLRMLERLTALGRPLVLVNFSGSPMTFGAATQKAAAIVQAWYPGGEGGQ
                     AVAELLAGEFSPSGRLPLTFYRSVEQLPPFKDYGMQGRTYRWFKGETLFPFGHGLSYA
                     RFAYTQAKLTRSRVTAGQGTRLSVLLKNKSSREAAEVVQVYAQRSGAMAPERTLVAFR
                     RVVLRAGESRRLSFELDAKAMSVVQADGRRVVDGGPAWLWVGGGQPVAAVTGVRASGQ
                     MLKLQVTGRAELPPFSERAPQGNAAWRAPLPGARRAP"
     gene            complement(3042..4619)
                     /locus_tag="VFV25_10615"
     CDS             complement(3042..4619)
                     /locus_tag="VFV25_10615"
                     /inference="COORDINATES: protein motif:HMM:NF013400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-xylosidase"
                     /protein_id="HEY1393821.1"
                     /translation="MPRSILRPLAVALLSLGVCAAAIAAPPAAREVTLDRAAARKPMD
                     RFFDLSVGADFPGTTGRPENLVQLKTAVDELGFRYVRFHDIFHDALGTVRKVDGRITF
                     DFSGIDKLYDGLLARGIKPFVELGFTPSAMKTSDQSIFYWKGNSSHPQPGPWGELIEA
                     FARHLVQRYGADEVHQWLFEFWNEPNLAGFFEGADQKAYFDLYALTARTLKRVDPALK
                     VGGPATAGAAWVPEFLAYAKANNVPVDFVTTHTYGVTEGFFDEFGKSDRILAIDPNSI
                     VGDVRRNRQEIEASAFPGLPLYVTEWSTSYSPRDKVHDSYVTAPWILTKLRATRGYAQ
                     AMSYWTYSDLFEEPGPPDAPFHGGFGLMTREGVRKPAWFAYKYLYALRGNEIPSADDK
                     VLAAAEGDKTAVVAWDWQHPDQKGNSNSIFFGKPLPNGPAPAVKLALRNLKPGAYRLE
                     LRRTGYKANDAHTAYLEMGSPKQLDAAQLTQLQSLTQDLPGVKRTVNVDKNGLLNFTV
                     PMRSNDVTLLTLEPVKR"
     gene            complement(4677..6107)
                     /locus_tag="VFV25_10620"
     CDS             complement(4677..6107)
                     /locus_tag="VFV25_10620"
                     /inference="COORDINATES: protein motif:HMM:NF027814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HEY1393822.1"
                     /translation="MSTTASTSARSSPDSLHRRGFVRAAVGAGLATIAGVPAAGAASA
                     FATASNTAEPGLPFHAWAPTPPMGWNSWDAFGASVTEAEYMDNARILAERFLPFGYDI
                     ATVDIQWYEAGAKSSWYRPFAPLVLDAHGRPQPASNRFPSAACGRGFAALAAQVHGLG
                     LRFGVHLMRGIPRQAVGLELPIAGSKWRCHEVANPGSTCAWNTDMCGVNPDHPGAFDW
                     YRAWFAQLAEWGVDFVKVDDIAAPNYHAREAELIRRAIDATGRSIVLSLSPGPALLEQ
                     AAHLQAHANQWRVCNDFWDTWPQLRDNLANLVKWAPHARPGAWPDADMLPVGKIGLRN
                     YDGDGHGERDTRFTRDEQVLMLTAWTCARSPLILGNDLRRLDDWTLRLVTNPEVLALL
                     RVPGTREWHSTAQGHRFVCSGPEGRWVAWLNTGEAPWSAPVPAWLPDARRDCWARADL
                     PSGTRNLTVPPHGARLVRCDPPLEKA"
     gene            complement(6451..7722)
                     /locus_tag="VFV25_10625"
     CDS             complement(6451..7722)
                     /locus_tag="VFV25_10625"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEY1393823.1"
                     /translation="MKTIRAMACGTALAAWACGAHSQSEVVVLPTASDAWRITAVDGE
                     TRAELTGDSLVAGQLGVIVHGRDGVRDAVLFDWKDRWEAVLRFESLQPLDLRPFVAHG
                     TLEFDLDVAELAHGAIRVKLSCGEGCEPSVNIFEQGRAWAGKSWQHVALPISCFVRDG
                     ADFSRVKLPFGLIGVGSGRASVTNVRLVREGKPNAGCPDYRTESVTPVVQNEWWALDW
                     WMPRHQEKLEEKRKLVAAGTPPEIAFIGDSITQNWETAGREVWQHHYAKYHALDLGFG
                     GDRTENVLWRLQHGEIDSIAPKVAVLMIGTNNTGQRAEDPRTIAAGIRRIVDEIHRRT
                     PGTRVLLLAIFPRGVKPDDFLRGINERVNRLIAGWSDVYFLDITAAFLSPDGTLSKDV
                     MPDLLHPNEKGYAIWQRAMEPALRKLLEASP"
BASE COUNT         1176 a         2657 c         2829 g         1243 t
ORIGIN      
        1 aggctgggct gcgtggtgta ggcctggccc tggtgggctg tcacgcgcac caagccgggc
       61 ccgtagaaca gcacgctctt agtcagcccg ccgacgcgga actgcaggcc ttcggccacg
      121 cgctgccagg cgaagccttc aggccctgct tccatgggca gggccttcat ctgcccggcg
      181 gcgtccaggg cggtgggcga cccggaggcc gcggtggctg gccaagccag catgcccgcg
      241 gcgggggcac aggcgatcag gccgacaagg tcacgacgtt tcattcacgg gcctccagat
      301 tcatcgcggt ctgtcacggg gcgcggcgcg cgccgggcag cggtgcgcgc caggccgcat
      361 tgccctgtgg cgccctctct gaaaacggcg gcagctccgc acgacccgtg acctgcagct
      421 tcagcatctg cccggaggcg cgcaccccgg tgaccgcagc caccggctga ccgccgccaa
      481 cccacagcca cgccggcccg ccatccacca cgcgccgccc gtcggcctgg accacgctca
      541 tcgccttggc gtctagctcg aagctgagcc gacgtgattc acccgcgcgc agcaccacac
      601 gccggaaggc caccagcgtg cgctccggcg ccatggcgcc gctgcgctgg gcatagacct
      661 gcaccacctc ggccgcctca cgcgagctct tgttcttcaa cagcaccgaa agccgcgtgc
      721 cttgcccggc ggtgacgcgg ctgcgagtca gcttggcctg cgtataagca aagcgcgcat
      781 aactcaggcc gtgaccgaac gggaacaagg tctcgccctt gaaccaacgg taagtgcgtc
      841 cctgcatgcc gtagtccttg aacggcggca actgctcgac cgaccggtag aaggtgagcg
      901 gcagccggcc cgaaggactg aattcgccgg ccagcagttc ggccaccgcc tggccgccct
      961 cgccacccgg gtaccaggct tgcacgatgg ctgccgcctt ctgggttgcg gcgccaaagg
     1021 tcatcgggct gccgctgaag ttcaccagca ccagcgggcg gcccagcgcc gtcaggcgct
     1081 ccagcatccg tagttgcgga gccggcagtt cgatctgggt gcggtcaccg ccggcaaagc
     1141 cgggggccag caccgtcatt tcttcccctt ccagctccca ggtcaggccg ctggcaaaca
     1201 ccaccaggtc ggcctcgcgc gcggccgcca cggcggcttc atcgctggcg ccggggcggc
     1261 tccactgcag gcgctgctca ccgtcccagc cggtctgcag cgcctccacg cgcagggcgt
     1321 aggtctggcc cgccttcagc atcacgccgc gggtgctggt gggccaggcg atgtcccaca
     1381 ggtccaccac cagttcaccg tcgatccaga tgcggtagcc gcggttgccc ttcagccgga
     1441 acaggtgctt gccgctttcg ccggcgcgca gaaagccggt ccagcgtgcc gattccttgc
     1501 ggtcgtaggc atccggccag ccccacttga acacggcctg cggcgtgagt gcagccgaac
     1561 tgggtgcccc ctcgagcttg gtgttggcga agcgctcgaa gtgcaggccg gcgcggctgc
     1621 aggcggcgtc ggcacacagg ctggacgccg gcaggtcttc cagtggcggc gccacccaac
     1681 ccgtgccctc gacgaaatcg atgcgcgcgc cgggaaagcg cgccttcagg ccagccagca
     1741 gcgtgatcgg cttggacggc gtgccgttgt agttgcccac cagcgcatcc acgctgtcgg
     1801 cattggggcc gatcacggca atgcgccggg gcggggcctt cagcggcagc acgccatcgt
     1861 tcttgaggag caccagcgag cggcgggccg actccaggtt gagcgcgcgg tgcgccgggg
     1921 tgtcgaagtc gtgcgtcgtg atctcgggaa aaggccgctg cccggcggag gcgagcaagc
     1981 ccaggcgcag gcgcacgtcg aacaggcggt gcagcgcccg gtcgagttcg gcttcgctca
     2041 gcagcccttg ctgcacggcc gccacggtgg tggcgggcgc ggcagtcttg ttgccgccaa
     2101 agtcacagat caggtcggtg ccggcgcgca cggccagggc cacggcctcc tcaggcgtct
     2161 tcgcatggtg gtgcgcccaa tccatgtaga tgtcggccac ggcgccgcag tcggacacca
     2221 catggccctg gaacttccac tcgtcgcgca gatagtgctg aagcaagcgc tcgttggcac
     2281 aggcgggcac gccgtccacg gcgttgtagg cgcacatcac cgattcggcc cggccttcgg
     2341 tgaccgccgc atggaaggcc ggaaggtagg tgtccaccag gtcgcggggc gagaccttca
     2401 cgtcctctcg gtggcggtcg gcctccgggc cgctgtgcac cgcgaagtgc ttcacggccg
     2461 ccgacacacg cggctggttc gcatccggcc cctgcagccc ctggatgaag gccacgccca
     2521 tgcgcgaggt caggtacggg tcttcgccat aggtttcctg gccgcggccc cagcgcgggt
     2581 cgcgaaagat gttgacgttg ggcgaccaca ccgtcaggcc gcggtacagc ccggacgagc
     2641 catcggcacc gaccgtgttc aggaacttgg cgcggaactc ggtgctgatg acatcgccca
     2701 cgcgctgcac gagcggcgcg tcccaggttg ccgccaggcc gatggcctgc gggaacacgg
     2761 tggccacgtc ggcgcgcgcc acgccgtgca gtccctcgct ccaccagttg tacgcaggga
     2821 tgcccaggcg cgggatggcc ggcgagtcgt gctgcaactg gctggccttc tcggccagcg
     2881 tcatctgcgc caccaaggct tgcgccctct gcatcgaggg gctcagggga gcggccgcca
     2941 cgcaggtggc cagcgacaag gccagggcgg acgccacgat aagacagctg ttcatgggaa
     3001 gtatcgtcat gcgagcggtg accgcagcgg ctgcgccttg gtcagcgctt caccggctcc
     3061 agcgtcagca gcgtgacgtc gttgctgcgc atcggcacgg tgaagttcag caggccgttc
     3121 ttgtcgacgt tgacggtgcg cttcaccccg ggcaggtcct gcgtcagcga ctgcagctgg
     3181 gtgagctggg cggcgtcgag ttgcttgggc gagcccatct cgaggtaggc ggtgtgggcg
     3241 tcgttggctt tgtagccggt gcggcgcaac tccagccggt aggcgccggg cttgaggttg
     3301 cgcagcgcca gcttgaccgc cggcgccggg ccgttgggca acggcttgcc gaagaagatg
     3361 ctgttgctgt tccccttctg gtcgggatgc tgccagtccc aggccacgac ggccgtcttg
     3421 tcgccttcgg cggcggccag caccttgtcg tcggcgctcg ggatctcgtt gccgcgcagc
     3481 gcgtagaggt acttgtaggc gaaccaggcc ggcttgcgca cgccctcacg tgtcatcagc
     3541 ccgaagccgc cgtggaaggg cgcgtcgggc ggccccggct cctcgaacag gtcgctgtag
     3601 gtccagtagc tcatcgcctg cgcgtagccg cgcgtggcgc gcagcttggt caggatccag
     3661 ggcgcggtga cgtagctgtc gtgcaccttg tcgcgcgggc tgtagctcgt gctccactcg
     3721 gtcacgtaga gcgggaggcc ggggaaggcc gaggcctcga tctcctgccg gttgcggcgc
     3781 acgtcgccga cgatcgagtt cgggtcgatc gcgaggatgc ggtcggactt gccgaattcg
     3841 tcgaagaagc cttcggtcac gccgtaggtg tgggtggtga cgaagtcgac cgggacgttg
     3901 ttggccttgg cgtaggccag gaactcgggc acccaggcgg cgccggcggt ggcggggccg
     3961 ccgaccttga gtgccggatc gacgcgcttg agagtgcgcg ccgtcagtgc gtagagatcg
     4021 aagtaggcct tctggtcggc accttcaaag aagccggcga ggttgggttc gttccagaac
     4081 tcgaacagcc attggtgcac ctcgtcggcg ccgtagcgct ggaccaggtg gcgcgcgaag
     4141 gcctcgatca gctcgcccca gggcccgggc tgcgggtgcg agctgttgcc cttccagtag
     4201 aagatcgact ggtccgaggt cttcatcgcc gacggcgtga agcccagttc gacgaagggc
     4261 ttgatgccgc gcgcgagcaa gccgtcgtag agcttgtcga tgccgctgaa gtcgaaggtg
     4321 atgcggccat cgaccttgcg cacggtgccg agcgcgtcgt ggaagatgtc gtggaagcgg
     4381 acgtagcgga accccagctc gtcgaccgcg gtcttgagct gcaccaggtt ctccggccgg
     4441 ccggtggtgc cggggaagtc ggcgccgacg gacaggtcga agaagcgatc catgggcttc
     4501 ctcgccgcgg cgcggtccag cgtcacctcc cgggcggcgg gtggcgcggc gatggccgcg
     4561 gcgcacacgc caagggaaag cagcgccacc gcgagcgggc gcaggatgga acgcggcacg
     4621 ggaagcaaag aggtggtcat ctgaggaggt cgggagggaa aggtgaaggg ttcatgtcag
     4681 gctttctcca gcggcggatc gcagcgcacg aggcgcgcac cgtgcggcgg cacggtgagg
     4741 ttgcgggtgc cggagggcag gtcggcgcgc gcccagcagt cgcggcgcgc gtcgggcagc
     4801 caggcgggca caggcgcgct ccacggggcc tcgccggtgt tcagccatgc cacccagcgg
     4861 ccctccgggc cgctgcagac gaagcggtgg ccttgtgcgg tgctgtgcca ctcgcgcgtg
     4921 ccgggcacgc gcagcagcgc cagcacttcg gggttggtga ccaggcgcag cgtccagtcg
     4981 tcgagccggc gcaggtcgtt gcccaggatc agcgggctgc gcgcgcaggt ccaggcggtg
     5041 agcatcagca cctgctcgtc gcgcgtgaag cgggtgtcgc gctcgccgtg gccgtcgccg
     5101 tcgtaattgc gcaggccgat cttgcccacc ggcagcatgt cggcatcggg ccaggcgccg
     5161 gggcgcgcgt gtggtgccca cttcaccagg ttcgccaggt tgtcgcgcag ctgcggccag
     5221 gtgtcccaga agtcattgca cacccgccat tggttggcgt gcgcctgcag gtgcgccgcc
     5281 tgttccagca gtgccgggcc gggcgacagg ctcagcacga tggagcggcc ggtggcgtcg
     5341 atggcgcggc ggatcagctc cgcctctcgt gcgtggtagt tgggcgcggc gatgtcgtcg
     5401 accttgacga agtccacgcc ccattccgcg agctgcgcga accaggcgcg gtaccagtcg
     5461 aaggcgccgg ggtggtcggg gttgacgccg cacatgtcgg tgttccacgc gcaggtcgag
     5521 cccgggttgg ccacttcatg gcagcgccat ttgctgccgg cgatgggcag ctccaggcct
     5581 accgcctggc gcgggatgcc gcgcatcagg tgcacgccga agcgcagccc caagccgtgc
     5641 acctgcgcgg ccagcgctgc gaacccgcgg ccgcaagcgg cgctagggaa gcggttggat
     5701 gctggctgcg ggcggccatg ggcgtccagc accagcggcg cgaagggtcg gtaccacgac
     5761 gatttggcgc ccgcctcgta ccactggatg tcgacggtgg cgatgtcgta gccgaagggc
     5821 aggaagcgct cggcgaggat gcgcgcgttg tccatgtact cggcctcggt gacgctcgcg
     5881 ccgaaggcgt cccagctgtt ccagcccatc ggcggcgtgg gcgcccaggc gtggaaaggc
     5941 aggcccggct cggccgtgtt ggaggctgta gcgaacgcgc tggcggcgcc ggccgcgggc
     6001 acgccggcaa tggtggccag cccggcgccg acggcagcgc gcacgaagcc gcggcggtgc
     6061 agcgagtctg gcgagctgcg tgcagaagtc gatgcagtgg tgctcattgg gcatcctccc
     6121 aggtattgac accggtgagg agaaccgccg atggtgacca ccttccgtgc cttgcggtga
     6181 ctgcctgtgg tgcttgcctt tcgaaggccc tcatgagacc cgcagcaccg tgaggtgccg
     6241 gaaggccatg acgggccacc ggatgtagcg ccacaccttg gaagtgcggc gagcatcttc
     6301 gctgctgtcg attcgcccat cgccctggca ttccgatcgg gccgaacatc acggccacga
     6361 cgtcgtttgc gccgggcggc ggctggatgc gcagccgcat cggcagcggg ctcgtgcgca
     6421 cctgggacgt gcccgcgcag gggagcgtgt tcatggggat gcttccagaa gcttccgcag
     6481 cgcgggttcc atcgcgcgct gccagatcgc gtagcccttc tcgttggggt gtagcagatc
     6541 gggcatcacg tccttcgaca gcgtgccgtc ggggctcagg aacgcggcgg tgatgtcgag
     6601 gaagtagaca tcgctccagc ctgcgatgag gcggttcaca cgctcgttga tgccgcgcag
     6661 gaagtcgtcg ggcttcacgc cgcgcgggaa gatggcaagc agcagcacgc gcgtgccggg
     6721 cgtgcggcga tggatctcgt cgacgatgcg gcggatgccg gcggcgatgg tccgcggatc
     6781 ctccgcgcgt tggccggtgt tgttggtgcc gatcatcagc accgcgacct tcggcgcgat
     6841 gctgtcgatc tcaccgtgct gcaggcgcca cagcacgttc tcggtacggt cgccaccgaa
     6901 gccgaggtcc agcgcgtggt acttcgcgta gtggtgctgc cagacttcgc ggccggcggt
     6961 ctcccagttc tgggtgatgg aatcaccgat gaaggcgatc tccggcggcg tgccggcggc
     7021 aacgagcttg cgcttttctt ccagcttctc ctggtggcgc ggcatccacc agtccagcgc
     7081 ccaccattcg ttctggacga caggcgtcac ggactcggtg cggtagtcag ggcagccggc
     7141 gttaggcttg ccctcgcgca cgaggcgcac gttggtgacg ctggcgcgcc cgctgccaac
     7201 gccgatcagc ccgaacggca gcttgacgcg cgagaagtcg gcgccatcgc gcacgaagca
     7261 actgatcggc agcgccacgt gctgccagct cttgcccgcc caggcgcgac cctgctcgaa
     7321 tatgttcacc gagggctcgc agccttcgcc gcaggacagc tttacccgga tcgcgccatg
     7381 cgcgagctcg gcgacgtcga ggtcgaactc gagcgtgccg tgggccacga aggggcgcag
     7441 gtccagcggc tgcaagctct cgaagcgcag cacggcctcc cagcggtcct tccagtcgaa
     7501 cagcacagca tcgcgcacgc cgtcgcggcc gtggacgatg acgccgagct gaccggcgac
     7561 gaggctgtcg ccggtcagct cggcccgggt ttccccgtcg acggcggtga tgcgccaggc
     7621 gtcggatgcg gtgggcagca caacgacctc ggactgggag tgcgcgccgc acgcccaggc
     7681 ggccagcgcg gtgccgcagg ccatggcacg gatcgtcttc atcgcggtgg actcaccgac
     7741 tcgcccttct cgtgacatcg ccatcacagc accggcgtga tgcgcaccat gaccaccccg
     7801 tgcgaggcca cggtgcggcc gtagctaccg tgcacgccct ttgtgacctt cttgctccac
     7861 aggtccttca cgtcggcctt caggccaacg ggcaggttga gcagc
//
LOCUS       DASUCY010000539         5329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1056641, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000539 DASUCY010000000
VERSION     DASUCY010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 5329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5329
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1056641"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VFV25_10630"
     CDS             <1..660
                     /locus_tag="VFV25_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEY1393824.1"
                     /translation="AARQALQAAFGDWKSPSAFLRVPQPATPEQPVRLVLATPDKQNA
                     TMLVRLSLPITDNDADYPALYIANWVLGAGGDSRLWKRIREKEGLSYGVGSYVRWNNF
                     EPNSSWNAYAIFAPQNRDKVETGFKEEVARALKDGYGSEEVEAAKKGALSARRLGRAQ
                     DANLAGALAGNLYLGRDFALSQRIDDAIAKLTADQVNAALRKYLKPESFVYGLGGDFK
                     N"
     gene            complement(672..1031)
                     /locus_tag="VFV25_10635"
     CDS             complement(672..1031)
                     /locus_tag="VFV25_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393825.1"
                     /translation="MGTGIEAALVKPIVDALVALYKKAKSTHLKSTAEAALSEAIREL
                     LLAPSDLRSAEAKIAVAKAAGIINADLVLAEEMVTKHKAAGKKVPAKKPAAKKAVAVK
                     KAAAGKPAAKKPAAKRA"
     gene            complement(1035..1661)
                     /locus_tag="VFV25_10640"
     CDS             complement(1035..1661)
                     /locus_tag="VFV25_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEY1393826.1"
                     /translation="MDRAGGHAMKSCSALLAEVRACRLCAEHLPLGPRPVLQFHPGAR
                     ILIAAQAPGRKVHESGLPFDDASGDRLRAWLGLDRKTFYDERRIAILPMAFCYPGKGR
                     TGDLPPRPECAPAWRAPLLSQLKHVELTLVIGRYALAYHLPATHGSVTEAVTAWRDHW
                     PAVVPLPHPSPRNQLWLRRHPWFEQYLLPLLRMRVAEVLGFAPEMSRR"
     gene            complement(1634..2197)
                     /locus_tag="VFV25_10645"
     CDS             complement(1634..2197)
                     /locus_tag="VFV25_10645"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY1393827.1"
                     /translation="MPEPVTLRSDRLTIRPLLETDLAGVFALYDNVDVGRFCKPVRWP
                     SMDYAHGWYARIEQRVAEGSTLQFVIELAQQQTLIGTCLIFQIDEENRRAEVGYALGR
                     DFWGRGYASEALQALIEHGFGTLGLHRLEAVIDPRNTASAKVLTRHGFRLEGQQRERW
                     IESDGSFGDSGLYGLLRQEWTGPAAMR"
     gene            complement(2261..2734)
                     /locus_tag="VFV25_10650"
     CDS             complement(2261..2734)
                     /locus_tag="VFV25_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY1393828.1"
                     /translation="MSETITLDRTLRRIIHELQHDGALSAQQLAERVGLSATPVWRRV
                     RELERTGVLRRHVALMDREKLGLAICVLAYVTLSGHRQGVVEDFERFVRACPEVMECA
                     SITGDADFVLKVVAADMKAYDRFLNERLFKQTGVASVRSNVVLREVKYETALPVA"
     gene            2843..>5329
                     /locus_tag="VFV25_10655"
     CDS             2843..>5329
                     /locus_tag="VFV25_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEY1393829.1"
                     /translation="MLDPNRAADSALRDYRLADNLAASSGQVFLTGTQALVRLLLMQR
                     RLDEAQGLRTAGFVSGYRGSPLGMVDQQLWKAQKLLDAAGVNFLPAINEDLAATACLG
                     TQRVALDPKRTVDGVYAMWYGKGPGVDRSGDALKHGNAYGSSAQGGVLVVAGDDHGCV
                     SSSMPHQSDLALQAWSMPLLHPANVAEYLEFGLYGWALSRFSGAWVGFKAISEVVESG
                     MTVDLDAIPLDFELPVDYTPAQPLHVRAVDLPSLAIEERLALKLDAVRAFARLNSVDK
                     HIVASPNATLGIVTVGKAHFDFMEVLRRLDLDPNALAAAGVRVYKVGLVFPLEPTRMR
                     EFAQGLDELLVIEEKAPVVERQLKELLYDLPDHQRPRIAGKADPRGAPLLSALGELRP
                     SRVMPVIAEWLARLNPALDRRHLVVDFTMAALLSNAADAVRRQPYFCSGCPHNTSTKV
                     PEGSRALAGIGCHFMASWMERETSGLIQMGAEGVDWAAHSRFTTEKHVFQNLGDGTYF
                     HSGLLAIRQAIAADATITYKLLYNDAVAMTGGQPVDGHISVPEIARQVEAEGAKRVAV
                     VSDDIAKYRGHEGEFPRGTSFHERAEMDAVQRELREVEGVTLLIYDQTCAAEKRRRRK
                     KGEMVDPAKRVFINERVCEGCGDCGVASNCLSIIPVETDWGRKRQIEQSSCNKDYSCV
                     NGFCPSFVSVHGAVLRKHAGSDFGTADLERELAAIAAPAPWQWTGPFDLLVTGVGGTG
                     VVTVGALVAMAAHLEGKQASVLDFMGFAQKGGAVLSFVRVAPTADQLNQVRIDTQQAD
                     VLLACDMVVGASADALGTVKHGRTVVLANTH"
BASE COUNT          861 a         1814 c         1798 g          856 t
ORIGIN      
        1 gccgcgcggc aagccttgca agccgccttc ggcgactgga agagcccaag cgccttcctg
       61 cgcgtgcccc agcccgccac gcctgagcag ccggtgcgcc tggtgctcgc cacgcccgac
      121 aagcagaacg cgacgatgct ggtgcgcctg agcctgccca tcaccgacaa cgatgccgat
      181 taccccgccc tctacatcgc caactgggtg ctgggcgcgg gcggcgactc ccggctgtgg
      241 aagcgcatcc gcgagaagga gggcctgtcc tacggcgtgg gcagctatgt gcgctggaac
      301 aacttcgaac ccaattccag ctggaacgcc tatgcgatct ttgcgccgca gaaccgtgac
      361 aaggtcgaga ccggcttcaa ggaggaagtc gctcgcgcgc tgaaggacgg ctacggcagc
      421 gaggaagtgg aggcggccaa gaagggcgct ttaagcgcgc gccggctcgg ccgcgcccag
      481 gacgcgaacc tcgcaggcgc gctggccggc aacctctacc tgggccgcga cttcgcgctg
      541 tcgcagcgca tcgacgacgc gatcgccaag ctcacggcgg atcaggtcaa cgcggctttg
      601 cgcaaatacc tgaagccgga gagcttcgtc tacggcttgg ggggcgactt caagaactga
      661 agcgaagagc cttaggcgcg cttggccgcc ggcttcttgg cggcaggctt gccggccgct
      721 gccttcttta cggcgacggc cttctttgcg gccggcttct tcgccgggac cttcttgccg
      781 gcggccttgt gcttggtgac catttcttcg gcgagcacga gatccgcgtt gatgatcccc
      841 gccgccttgg ccacggcgat cttcgcctcg gcggatcgca ggtcggacgg cgccagcagc
      901 aattcgcgta tggcttcgct gagcgctgcc tccgcggtcg acttcaggtg cgtgctcttg
      961 gccttcttgt acagagcgac caaggcatcg acgatgggct tgacgagcgc cgcctctatg
     1021 ccagttccca aggatcacct ccggctcatt tcgggtgcga agcctagcac ctcggcgacg
     1081 cgcatccgca gcaggggcag taggtattgc tcgaaccaag gatggcgcct gagccacagc
     1141 tggttgcgcg ggctggggtg gggcagcggc acgaccgcgg gccaatgatc gcgccaggcg
     1201 gtgacggctt cagtgacgga accatgggtg gcgggcaggt gataggctag cgcgtaacgc
     1261 ccgatcacca gcgtcagctc cacatgcttg agctggctca gcagcggcgc ccgccaggcc
     1321 ggcgcgcatt cggggcgggg cggcagatca ccggttcggc ccttgcctgg atagcagaag
     1381 gccatcggca ggatcgcgat gcggcgctcg tcatagaaag tcttgcgatc cagccccagc
     1441 caggcgcgca ggcggtcgcc gctcgcgtca tcgaagggga ggccgctttc atgcaccttg
     1501 cggcccgggg cctgggctgc gataaggatg cgggcgcctg ggtggaactg cagcacgggc
     1561 cgtgggccca gcggcagatg ctcggcgcag agccggcagg cgcgaacctc ggccagcagc
     1621 gccgagcagg acttcatcgc atggccgccg gccctgtcca ttcctgcctc agcaagccat
     1681 agagcccgga gtcgccgaag ctgccatcgc tctcgatcca gcgctcacgc tgctggcctt
     1741 cgagcctgaa gccatggcgc gtcagcacct tcgccgatgc cgtgttgcgt gggtcgatca
     1801 cggcttccag gcgatgcagg cccagcgtgc cgaagccatg ctcgatcagc gcttgcagcg
     1861 cctcgctcgc atagccgcgg ccccagaaat cgcgccccag cgcatagccg acctcggctc
     1921 gcctgttctc ctcgtcgatc tggaagatca aacaggtgcc gatcagcgtc tgctgctgcg
     1981 ccagctcgat gacaaactgc agcgtgctac cctcggccac gcgctgctca atgcgggcgt
     2041 accagccatg cgcatagtcc atcgacggcc agcgcaccgg cttgcagaag cggccgacgt
     2101 cgacgttgtc atagagcgcg aagacgccgg cgagatcggt ttcgagcagc gggcgtatgg
     2161 tcaggcggtc tgagcggagg gtgacgggtt caggcatagg gactggtggc caggaatgga
     2221 ccgcgcagtc tacggccagc cagccgcggg cagctgtctc tcacgccacc ggcagcgccg
     2281 tctcgtactt cacctcgcgc agcacgacat tgctgcgcac gctggccacg ccggtctgct
     2341 tgaacagccg ctcgttgagg aagcggtcgt aggccttcat gtccgcggcc accaccttaa
     2401 ggacgaagtc ggcgtcgccg gtgatgctgg cgcattccat gacttcggga caggcgcgca
     2461 cgaagcgctc gaagtcttcg acgacgccct ggcggtgccc ggacagggtc acataggcca
     2521 gcacgcagat cgcgagcccg agcttttcgc gatccatcag ggccacgtgc cgccgcagca
     2581 cgccggttcg ctccagctcc ctgacccgcc gccagacggg tgtcgccgac agccccacgc
     2641 gctcggcaag ctgctgggcg ctgagcgcac catcgtgctg cagctcgtgg atgattcgcc
     2701 gtagggtgcg gtcgagggtg attgtttcac tcatggcgag tagtggaaat gaacattctt
     2761 atgattggcg aaatggcgca atgtaaggaa cactcgtttt cccggcaagg cctaggattt
     2821 cgcgaacgcc gaggagcccg ccatgctcga tcccaaccgt gccgccgatt cggcactacg
     2881 tgactaccgt ctcgctgaca atctcgccgc gtcctccggc caggtcttcc tgaccggcac
     2941 ccaggcgctg gtgcgcctgc tgctgatgca gcgccgcctc gacgaggccc agggcctgcg
     3001 caccgccggc ttcgtgtccg gctatcgcgg cagcccgctc ggcatggtcg accagcagct
     3061 gtggaaggcc cagaagctgc tcgacgcggc cggcgtgaac ttcctgccgg cgatcaacga
     3121 agacctcgcc gccaccgcct gcctgggcac gcagcgcgtg gcgctggacc ccaagcgcac
     3181 ggtcgatggc gtctatgcga tgtggtacgg caaaggaccg ggcgtggacc gctccggcga
     3241 cgcgctcaag catggcaatg cctatggcag cagcgcccag ggcggcgtgc tggtggtagc
     3301 gggcgacgac cacggctgcg tctcctcgtc gatgccgcat cagtccgacc tcgcgctgca
     3361 ggcctggagc atgccgctgc tgcacccggc caatgtcgcc gagtacctgg aattcggcct
     3421 ctacggctgg gccttgtcgc gcttctcggg cgcctgggtg ggcttcaagg cgatctccga
     3481 ggtcgtcgag tcgggcatga cggtcgatct cgacgccatt ccgctcgact tcgaactgcc
     3541 ggttgactac acgccggccc agccgctgca tgtacgcgca gtggacctgc catcgctcgc
     3601 gatcgaggag aggctcgcgc tcaagctcga cgcggtgcgc gccttcgcgc ggctcaattc
     3661 ggtggacaag cacatcgtgg ctagcccgaa cgccacgctg ggcatcgtca cggtgggcaa
     3721 ggcgcatttc gacttcatgg aggtgctgcg ccggctcgat ctggacccca atgcgctggc
     3781 cgcggccggc gtgcgcgtct acaaggtcgg gctggtgttt ccgctggaac cgacacgcat
     3841 gcgcgagttc gcgcaggggc tggacgagct cctggtcatc gaggagaagg cgccggtcgt
     3901 cgagcgccag ctcaaggaac tgctctacga cctgcccgac caccagcgcc cgcgcatcgc
     3961 cggcaaggcc gacccgcgcg gcgcgccgct gctgtcggcc ctgggcgagc tgcggccctc
     4021 gcgcgtgatg ccggtgatcg ccgagtggct ggcgcggctg aacccggcgc tggaccgccg
     4081 tcatctggtg gtcgatttca cgatggcggc gctgctgtcc aacgcggccg acgcggtgcg
     4141 ccgccagccc tacttctgct ccggctgccc gcacaacacc agcaccaagg tgccggaggg
     4201 ttcgcgcgcg ctggccggca tcggctgcca cttcatggcg agctggatgg agcgcgagac
     4261 ctcgggcctg atccagatgg gcgccgaggg cgtcgactgg gccgcgcatt cgcgcttcac
     4321 gaccgagaag cacgtgttcc agaacctcgg cgacggcacc tacttccatt cggggctgct
     4381 ggcgattcgc caggccatcg ccgccgacgc cacgatcacc tacaagctgc tgtacaacga
     4441 cgcggtcgcg atgaccggcg gccagccggt ggacggccac atcagcgtgc ccgagatcgc
     4501 gcgccaggtg gaggccgaag gcgcgaagcg ggtcgccgtc gtctccgacg acatcgccaa
     4561 gtaccgcggc cacgaaggcg agttcccgcg cggcaccagc ttccacgagc gcgccgagat
     4621 ggacgccgtg cagcgcgaac tgcgcgaggt cgaaggcgtc acgctgctga tctacgacca
     4681 gacctgcgcc gccgagaagc gccgccgccg caagaagggc gagatggtgg atccggccaa
     4741 gcgcgtcttc atcaacgagc gcgtctgcga gggctgcggt gactgcggcg tggcctccaa
     4801 ctgcctgtcc attatcccgg tcgagaccga ttggggccgc aagcgccaga tcgagcagag
     4861 ctcctgcaac aaggactatt cctgcgtcaa cggcttctgc cccagcttcg tctcggtgca
     4921 cggtgcggtg ctgcgcaagc atgcgggctc ggacttcggc actgccgacc tcgagcgcga
     4981 gctggccgct atcgcggcgc ccgctccttg gcaatggacc ggccccttcg acctgctggt
     5041 caccggcgtg ggcggcacag gcgtcgtcac ggtgggcgcg ctggtcgcca tggccgcgca
     5101 cctggagggc aagcaggcct cggtgctcga cttcatgggc ttcgcgcaga aaggcggggc
     5161 ggtgctgagc ttcgtgcgcg tcgcgccaac ggcagaccag ctcaaccagg tgcgcatcga
     5221 cacccagcag gccgacgtgc tgttggcctg cgacatggtg gtcggcgcct cggccgatgc
     5281 gctgggcacg gtcaagcacg gccgcactgt ggtcctcgcc aacacgcac
//
LOCUS       DASUCY010000540         2508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1249350, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000540 DASUCY010000000
VERSION     DASUCY010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249350"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="VFV25_10660"
     CDS             <1..788
                     /locus_tag="VFV25_10660"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEY1393830.1"
                     /translation="IAIEDHYKPRFAGDALPRNHTGTVLALADKLETLVGLFGIGQLP
                     TGDKDPFALRRHALGVIRLLTERNLPLSLPALVDAAVPVFGERIADPSAALLDFFFDR
                     LAVSLREQGYSAQEVDAVLALRPERLGELPRRLAAVRAFAGLPESASLAAANKRVGNI
                     LKKAEGAVDARVDAELLKEPAEIALNDALKSVATKAENAWVTGNYTENLQVLAALKAP
                     VDSFFDDVMVNAEDPALRANRLGLLATLHAAMNRVADLSRLAA"
     gene            804..1439
                     /gene="gmhB"
                     /locus_tag="VFV25_10665"
     CDS             804..1439
                     /gene="gmhB"
                     /locus_tag="VFV25_10665"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEY1393831.1"
                     /translation="MADHTMKLVILDRDGTINEDRDDYVKSPEEWVPLPGALEAVARL
                     NHAGWHVVVASNQSGLGRGLFDMAALNAMHAKMHHALAKVGGRIDAVFFCPHEPDAHC
                     SCRKPLPGLFEQIAERYGIDLKGVPNVGDTPRDLQAGVPLGCEPHLLLTGKGARCDEA
                     QLTQLRQQLPELRIHKDLGAFADFLILRERRARAGAKGLPEADDSRPGELL"
     gene            1439..2221
                     /locus_tag="VFV25_10670"
     CDS             1439..2221
                     /locus_tag="VFV25_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY1393832.1"
                     /translation="MYRLRSLLFVLWMAVTVVPFALAAMLLSIFVRGDPVYWVCVTWL
                     RGCIWGARVICGVRHRVQGMEHLPSADRLAPVILAPKHQSTWETFALPTLMSHPLCYV
                     FKRELLLIPFFGWAIGRLDMIHIDRSKRTEAWNKVAEQGRRLLGQGNWVIMFPEGTRT
                     PRGSQGVYKSGATRLAVATGVPVVPIAVTSARCWPRKSFVLRPGVIDISIGRPIPSQG
                     CDAESLMREVETWIEAEMRRLDPEAYAAAEPALQQAGGQRAA"
     gene            2304..>2508
                     /locus_tag="VFV25_10675"
     CDS             2304..>2508
                     /locus_tag="VFV25_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007747697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY1393833.1"
                     /translation="MATDKIIAMQAFVRVVQTGTFTKAAESLGMEKSQVSRLVQLLET
                     QLSTKLLTRTTRRMMLTTEGAQYF"
BASE COUNT          388 a          863 c          862 g          395 t
ORIGIN      
        1 ccatcgccat cgaggaccac tacaagccgc gcttcgccgg cgacgcgctg ccgcgcaacc
       61 acaccggcac cgtactggcg ctggccgaca agctcgagac cttggtcggc ctgttcggca
      121 tcggccagct gcccaccggc gacaaggacc cgttcgcgct gcgccgccat gcgctgggcg
      181 tgatccgtct gctgaccgag cgcaacctgc cgctgtcact gccggctctc gtcgacgcgg
      241 ccgtgcccgt gttcggcgag cgcatcgccg atccgagcgc tgcactgctg gacttcttct
      301 tcgaccgcct cgcggtgtcg ctgcgcgagc agggctatag cgcgcaggaa gtggacgccg
      361 tgctggcgct gcgccccgag cgtctgggcg agttgccgcg ccggctggcg gcggtgcgcg
      421 ccttcgcagg cctgcccgag tcggcgtcgc tggcggccgc caacaagcgc gtcggcaaca
      481 tcctgaagaa ggccgagggc gcggtggatg ccagggtcga tgcggagctg ctcaaggagc
      541 cggccgagat cgcgctgaat gatgctctga aatctgtagc aaccaaggcc gaaaatgctt
      601 gggtaaccgg caattacacc gagaatctgc aagtcctggc ggcgctgaag gcgccggtgg
      661 acagcttctt cgacgatgtg atggtcaacg ccgaagaccc tgccctgcgc gccaaccgcc
      721 tgggcctgct cgcgaccctg catgccgcga tgaaccgcgt ggccgacctg tcccgcttgg
      781 cggcctgaag catgcgtctc aacatggccg atcacacgat gaagctcgtc atcctcgacc
      841 gcgacggcac gatcaacgag gatcgcgacg actacgtgaa gtcgcccgag gaatgggtgc
      901 cgctgcccgg cgcgctggag gccgtcgcgc ggctcaacca cgcgggctgg cacgtggtgg
      961 tggcgagcaa ccagtcgggc ctgggccgtg gcctgttcga catggccgcg ctcaacgcga
     1021 tgcacgccaa gatgcaccat gccctggcca aggtcggcgg gcgcatcgac gcggtcttct
     1081 tctgcccgca cgagccggat gcgcattgca gctgccgcaa gcccctgccg ggcctgttcg
     1141 agcagatcgc ggagcgctac ggcatcgatc tgaaaggcgt gcccaatgtc ggcgacacgc
     1201 cgcgcgacct gcaggccggc gttccgctgg gctgcgagcc gcatctgctg ctgaccggca
     1261 agggcgcgcg ctgcgacgag gcccagctga cccagctgcg gcagcagctg cccgaactgc
     1321 gcattcacaa ggatctgggt gcctttgccg atttcctgat cctgcgcgaa cgccgcgccc
     1381 gcgccggtgc caagggcctg cccgaggcag acgacagccg cccgggggag ctgctctgat
     1441 gtaccgcctg cgctcgctgc tcttcgtgct gtggatggcg gtcacggtcg tgcccttcgc
     1501 cctggccgcg atgctgcttt cgatcttcgt gcgcggcgat ccggtctact gggtctgcgt
     1561 gacctggctg cgcggctgca tctggggcgc gcgcgtgatc tgcggcgtgc gccatcgtgt
     1621 gcagggcatg gagcacctgc cgtcggccga tcgcctggcg ccggtgatcc tcgcgcccaa
     1681 gcaccagagc acctgggaga ccttcgcgct gccgacgctg atgtcccacc cgctgtgcta
     1741 tgtcttcaag cgcgagctgc tgctgatccc cttcttcggc tgggccatcg gccgcctgga
     1801 catgatccac atcgatcgca gcaagcgcac cgaggcctgg aacaaggtgg ccgagcaggg
     1861 gcgccggctg ctcggccagg gcaactgggt catcatgttc cccgagggca cgcggacgcc
     1921 gcgcggcagc cagggcgttt acaagagcgg ggccacgcgg ctcgcggtgg ccaccggcgt
     1981 gccggtggtg cccatcgccg ttacctcggc gcgctgctgg ccgcgcaaga gcttcgtgct
     2041 gcgcccgggc gtgatcgaca tctccatcgg ccggccgatc ccttcgcagg ggtgcgacgc
     2101 cgagtcgctg atgcgcgagg ttgagacctg gatcgaggcc gagatgcgcc gcctcgatcc
     2161 cgaggcctac gcggcggccg agccggcctt gcagcaagcc ggcggccagc gcgcggcctg
     2221 agcccgctgc gccctgcggg cagcagacaa gcgcaaaagg atagaaactc ctgcagctgc
     2281 acgggcagca atggagtcgc gtcatggcta cggacaagat catcgcgatg caggccttcg
     2341 tgcgggtggt ccagaccggc accttcacca aggccgccga gtccttgggg atggagaagt
     2401 cgcaggtgag ccgcctggtg cagttgctcg aaacgcagct gagcacgaag ctcttgaccc
     2461 gcacgacgcg ccgcatgatg ctgacgaccg aaggcgcgca gtatttcg
//
LOCUS       DASUCY010000541         2279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_494157, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000541 DASUCY010000000
VERSION     DASUCY010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494157"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VFV25_10680"
     CDS             complement(<1..544)
                     /locus_tag="VFV25_10680"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEY1393834.1"
                     /translation="MSIDTAGSQQLLKDINRMALVRQLCANPGLSRADLATAVGLTKS
                     TVSLLVRELMAEGWLVEREVVATGDLGRRPTPLFIDPSRLLLLGAEVGIESVRVVASS
                     LTGDVLARAVTSYGSSKSAKACVGTLASTLLKVRRQLDEATHRIVGIGIGLPGGVDEA
                     RGFLHFAPNLGWRDVPVGTLL"
     gene            733..>2279
                     /locus_tag="VFV25_10685"
     CDS             733..>2279
                     /locus_tag="VFV25_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEY1393835.1"
                     /translation="MNVKAIRRIVIVGGGTAGWMTAAPLAQMLGKHAHEVVLIESAEI
                     GTVGVGEATLPPIRAYNQTLGLDGADFVRKTQATFKLGIEFKDWGRLGHRFFHGFGDF
                     GPPIAHRSSFTHWLRLHRAGSMPSYEEWSTATMMARHKRFTPPSGDRPSAANAYSYAF
                     HFDAGLYAAYLRDYAMQRGAQRIEGTIVDVELRPDDGFVAAVKLRDGRRIEGELFIDC
                     SGFRGLLIAGAMKSAFEDWSRWLPCNSALAVPSAKDERLTPYTTSTAHSAGWQWRIPL
                     QHRTGNGHVYCNGFVSDDEAASTLLQNLDGAALDTPRQLRFTTGRRREAWVKNVVAIG
                     LSSGFLEPLESTSIQLIMDGVGRLIELFPDRDFQPGLAAEFNRRMARQYEAIRDFIIL
                     HYKLTERGDSEFWRYCAAMPIPDTLQQQIELFRASGRVAIFDPDSFAEPSWISIMFGL
                     GLAPQAYDPFVDLIEEDALRTHFARLREAIANTVAAMPDHAHFIARHVKAGPVVQPGA
                     REAKPAVA"
BASE COUNT          373 a          796 c          748 g          362 t
ORIGIN      
        1 tgagcagcgt gcccaccggc acgtcgcgcc agcccaggtt gggcgcgaaa tgcaggaagc
       61 cgcgcgcttc gtccacgccg cccggcaggc cgatgccgat gccgacgatc cggtgcgttg
      121 cctcgtcgag ctgcctgcgc accttgagga gcgtgctggc cagcgtgccg acgcaggcct
      181 tggcgctctt gctcgagccg taactggtta ccgcgcgtgc gaggacgtcg ccggtgagtg
      241 agctcgcgac cacgcggact gactcgatgc ccacctcggc gcccaacagc agcaagcggc
      301 tcgggtcgat gaacagcggt gtggggcgcc ggccgagatc gcccgtggcg accacttcgc
      361 gctccacgag ccagccctcg gccatcagct cgcgcacgag caggctgacg gtggacttgg
      421 tcaagcccac cgcggtggca aggtctgccc gagacaggcc cgggttggcg cacagctggc
      481 gcaccagggc catgcggttg atgtctttga gcagttgttg gctgccggcg gtgtcgatgg
      541 acatggcgga ccccccttcc atggatagcg gttgcgcgat gaccgccatg gtagttgggg
      601 cgtcgaacga atgattgcgg tgtcggcata ttagtttggg catcgaacga attaaaacgg
      661 tataaacacc tagccacatc cgcccgactc gggcgcagac ttcgcgccgc tgtgcaagag
      721 gtagaagcaa ccatgaacgt caaggccatc cgcaggatcg tcatcgtcgg cggcggaacg
      781 gccggttgga tgaccgctgc gccgctggcc cagatgctgg gcaagcatgc gcacgaggtg
      841 gtgctgatcg agtctgccga gatcggcacc gtgggcgtgg gcgaggcgac gctgccgccg
      901 atccgcgcct acaaccagac cctgggcctg gacggcgccg acttcgtccg gaagacgcag
      961 gccaccttca agctcggcat cgagttcaag gactggggcc ggctggggca tcgcttcttc
     1021 cacggcttcg gcgacttcgg cccgccgatc gcccaccgct cgtccttcac gcactggctg
     1081 cggctgcacc gagccggcag catgccgtcc tacgaggaat ggtccaccgc gaccatgatg
     1141 gcgcggcaca agcgcttcac gccgccgagc ggcgaccggc cttcggcggc caatgcctac
     1201 tcctatgcct tccacttcga cgccgggctg tacgccgcct atctgaggga ctacgcgatg
     1261 cagcgcggtg cgcagcgcat cgaaggcacg atcgtcgacg tcgagctgcg gccggacgac
     1321 ggcttcgtcg ccgcggtgaa gctgcgcgac gggcgccgca tcgaaggcga gctattcatc
     1381 gactgctcgg gcttccgcgg cctgctgata gccggcgcga tgaaatcggc attcgaggac
     1441 tggagccgct ggctgccctg caacagtgcg ctggccgtgc ccagcgccaa ggacgagcgg
     1501 ctcacgccct acaccacgtc caccgcgcac tcggcaggct ggcagtggcg cattccattg
     1561 cagcaccgca ccggcaacgg ccacgtgtac tgcaacggct tcgtctccga cgacgaggcc
     1621 gcatccaccc tgctgcagaa cctggacggc gcggcgctgg acacgccgcg gcagctgcgc
     1681 ttcaccaccg gccggcggcg cgaggcctgg gtgaagaacg tggtggccat cggcctgtct
     1741 tccggcttcc tggagccgct cgaatccacg agcatccagc tcatcatgga cggcgtgggc
     1801 cggctgatcg agctgttccc cgaccgtgac ttccagcccg gcctggcggc cgagttcaac
     1861 cgccggatgg cgcggcaata cgaggctatc cgcgacttca tcatcctgca ctacaagctc
     1921 accgagcgcg gcgactccga gttctggcgc tattgcgctg ccatgcccat ccccgacacg
     1981 ctgcagcagc agatcgagct gttccgcgcc agcggccgcg tggccatctt cgaccccgac
     2041 agcttcgccg agccgtcgtg gatctccatc atgttcggcc tcggcctggc gccgcaggct
     2101 tatgacccct tcgtcgacct gatcgaggag gatgccctgc gcacccactt cgcccgcctg
     2161 cgcgaagcca tcgccaacac ggtggccgcc atgccggatc acgcccattt catagcccgc
     2221 cacgtgaagg caggtcctgt cgtgcagccc ggcgcgcgcg aggccaagcc agcggtcgc
//
LOCUS       DASUCY010000542         3615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1040647, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000542 DASUCY010000000
VERSION     DASUCY010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040647"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1241)
                     /locus_tag="VFV25_10690"
     CDS             complement(<1..1241)
                     /locus_tag="VFV25_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEY1393836.1"
                     /translation="MTATTTTSTVAAAPAEAGAPAQAQSRSVRPLSALLPFLRPYRTR
                     IALAGVFLVLAAVSTLVFPAALKSLIDQGLVAADPGQRVMALRGHFFALFGVGAALGV
                     FSAARFYMVSWLGERVTADLRNAVYAHVLEQSPEFFETTQTGEVLSRLTTDTTLVQTV
                     VGSSLSMGLRNTVMGIGALLMLVITNPYVMTQVLGILVLVVLPSLYFGRRVRKLSRAS
                     QDRVADSSAIAAEVLNAIPVVQSYTQEPREARRFDGATENAFTTAVRRTRVRAGLVAF
                     IITATFGALLWGLYQGTQAVIQGHVTAGHLGQTIVYVVILVSSVAVLAEVYGDLLRAA
                     GATERLMELLTARSPIATPARPLALPAPAGGSTLSFERVSFHYPSRPQTAALRDFSLD
                     VRPGEMVALVGPSGAGKSTVL"
     gene            1345..1836
                     /locus_tag="VFV25_10695"
     CDS             1345..1836
                     /locus_tag="VFV25_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016453438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY1393837.1"
                     /translation="MNRRCPLPVNFYERDAYVPQESLGWMMHRVLQSIIVQADKRFAE
                     HDLTNAQWKPLFKLAKRPDKCATVAELARDLQIDAGAMTRLLDRLEKKGLVKRVRSTE
                     DRRIVNIELTPEGDAAAAHVPGVLCDVLNGHLAGFSKTEWQALKTYLARMIENGEAMR
                     EEA"
     gene            1905..3395
                     /locus_tag="VFV25_10700"
     CDS             1905..3395
                     /locus_tag="VFV25_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY1393838.1"
                     /translation="MKANNLLSAQSKAAARRGAVLTGLTLLTALAAALVLAGCASTGN
                     IKPHAASLNADAVGLNTGAAAPVVAADWWQGFGDAALNQLIDKALADSPSLKLAQSRI
                     EQAQAAAEGVGAAERPQLNAELDANRQHFSKTGIYPPPLGGATLNIGTLQANGSWELD
                     FFGRNRSALDAAVGAQRAAEADAQAARVLLAANVTHGYFQLARLVEQRDVAQRSLGQR
                     EEILALVRERVQAGLDTSVELRQSEGSLPETRQQLEALDEQIALARHALAALTVQPPQ
                     ALDGLTPHLQPVQAVPLPAAVPADLLGRRADIAAARWRVEAANKGVASARASFYPNIN
                     LVAFAGFNSIGFGNLLKSDSKQYGFGPAFSLPIFDGGRLRANLHGKSAELDAAVESYN
                     SSLIDAVRDVADQIASLQSIDRQQTEQAAAQTSAESAYDLALQRYKAGLGTYLTVLTA
                     EGNVLAQRRQATDLKARQLDTQVALIRSLGGGYAAGADMQTAQASN"
BASE COUNT          646 a         1275 c         1199 g          495 t
ORIGIN      
        1 tgcagcaccg tgctcttgcc ggcgccgctg gggcccacca aggccaccat ctcgccaggg
       61 cgcacgtcga ggctgaaatc gcgcagcgcc gccgtctgcg ggcgcgacgg atagtgaaag
      121 ctcacccgct cgaagctcag cgtcgagcca ccggccggcg cgggcagcgc cagtggccgg
      181 gccggcgtcg cgatcggcga gcgcgccgtg agcagctcca tcaggcgctc ggtggcgccg
      241 gcggcgcgca gcagatcgcc atagacctct gccagcaccg ccaccgagct gaccaggatc
      301 accacgtaga cgatggtctg ccccagatgg ccggccgtga cgtggccctg gatcaccgcc
      361 tgcgtgccct ggtacaggcc ccagagcagg gcgccgaagg tggccgtgat gatgaaggcc
      421 acgagcccgg cgcgcacgcg ggtgcggcgc acggcggttg tgaaggcgtt ctcggtcgcg
      481 ccatcgaagc gccgggcctc gcgcggctcc tgggtgtagc tctgcaccac ggggatcgcg
      541 ttcagcacct cggccgcgat ggcgctggaa tcggcgacac ggtcctggct ggcgcggctg
      601 agcttgcgca cgcgccgccc gaagtagagc gagggcagca ccaccagcac caggatgccc
      661 agcacctggg tcatcacata ggggttggtg atgaccagca tcagcagggc gccgatgccc
      721 atcaccgtgt tgcgcaggcc catcgagagg ctggagccga ccacggtctg caccagcgtc
      781 gtgtcggtgg tcagccggct caggacctcg ccggtctgcg tggtctcgaa gaactcgggg
      841 ctttgctcca gcacgtgggc ataaaccgcg ttgcgcagat cggccgtgac gcgctcgccc
      901 agccagctca ccatgtagaa gcgcgccgcc gagaacacgc ccaaggcggc gccgacgccg
      961 aacagcgcaa agaagtgtcc gcgcaaggcc atcacgcgct ggcccgggtc ggcggcgacg
     1021 agaccctggt cgatcaggga cttcagcgcc gccgggaaca ccagcgtgct gaccgccgcc
     1081 agtaccagga agacaccggc cagggcgatg cgggtccgat agggccgcag gaaggggagc
     1141 agggcagaca gcggccgcac cgagcggctc tgggcttgcg ctggcgcgcc ggcttcggcc
     1201 ggcgcagcgg caacggtgct ggtcgtggtg gtggcggtca tgctgggcag tgtaggtggc
     1261 ggcgcgaagc ttgcacagga agagagtgcg caagcaaaca tagacttgac attgtttgtc
     1321 taggcaatta tagttgagcc catcatgaat cgccgctgcc cccttcccgt caatttctac
     1381 gagcgcgatg cctacgttcc ccaggaaagc ctgggctgga tgatgcatcg cgttctgcag
     1441 tccatcatcg tgcaggccga caagcgcttc gccgagcatg acctgaccaa tgctcagtgg
     1501 aagccgctgt tcaagctggc caagcggccc gacaagtgcg cgacggtggc cgagctggcg
     1561 cgcgaccttc aaatcgacgc cggcgccatg acgcgcctgc tggatcgcct ggagaagaag
     1621 ggcctggtca agcgcgtgcg ctccaccgaa gaccgccgca tcgtcaatat cgagctcacg
     1681 cccgaaggcg acgcggccgc ggcccatgtg ccgggcgtgc tgtgcgacgt gctcaacggg
     1741 catctcgccg gcttctccaa gaccgagtgg caggcgctca agacctatct ggcgcgaatg
     1801 atcgagaacg gcgaggcgat gcgcgaagag gcctgagggc ctcgtcgcca acagggtctt
     1861 ttgctatccg aataattgcc taagcaaaaa aagactggac aaccatgaaa gccaacaacc
     1921 tgctttccgc acaatccaag gctgccgccc ggcgcggcgc agtgctgacc gggctgaccc
     1981 tgctgaccgc gctggccgcg gccctggtgc tcgccggctg tgccagcacc ggcaacatca
     2041 agccccatgc cgcgagcctc aatgccgacg cggtcggcct gaacaccggc gccgccgcgc
     2101 ctgttgttgc tgccgactgg tggcaaggct tcggcgatgc cgcgctgaac cagctgatcg
     2161 acaaggctct ggccgacagc cccagcctca agctggcgca aagccgcatc gagcaggccc
     2221 aggcggccgc cgaaggggtg ggcgcggccg agcgcccgca actcaacgcc gagctcgatg
     2281 ccaaccgcca gcacttcagc aagaccggga tctatccccc gcccctgggt ggcgcgacgc
     2341 tgaacatcgg cacgctgcag gccaacggct cctgggagct ggacttcttc ggccgtaacc
     2401 gctccgcgct ggacgcggca gtcggcgcgc agcgcgcggc cgaggccgac gcccaggcag
     2461 cccgcgtgct cctggccgcc aacgtgacgc acggctactt ccagcttgct cgcctcgtcg
     2521 agcagcgcga tgtcgcgcag cgttcgctgg gccagcgcga agaaatcctg gccctggtgc
     2581 gcgaacgcgt gcaggcgggc ctggacacca gcgtggagct gcgccagagc gagggctcgc
     2641 tgcccgaaac gcgccagcag ctcgaagccc tcgacgagca gatcgcactg gcccgccacg
     2701 ccctggccgc gctcacggtg cagccgccgc aggccctgga tggcctcact ccgcacttgc
     2761 agcctgtgca ggccgtgccg ctgccggccg ccgtgcccgc cgacctgctg ggccgccgtg
     2821 ccgacatcgc ggccgcgcgc tggcgcgtcg aggcggcgaa caagggcgtt gcctcggccc
     2881 gcgcctcgtt ctatcccaat atcaacctgg tcgccttcgc cggcttcaat tcgatcggct
     2941 tcggcaacct gctcaagtcc gacagcaagc agtacggctt cgggccggcc ttcagcctgc
     3001 cgatcttcga cggcggacgc ctgcgcgcca acctgcacgg caagtcggcc gagctcgacg
     3061 cggccgtgga gtcctacaac agcagcctca tcgacgcggt gcgcgacgtg gccgaccaga
     3121 tcgcctcgct gcagtcgatc gaccgccagc agactgagca ggcggccgcg cagacgagcg
     3181 ccgagagcgc ctacgacctg gcgctgcagc gctacaaggc cgggctgggc acctatctca
     3241 ccgtgctcac cgccgaaggc aatgtgctgg cgcagcgccg ccaggccacc gacctcaagg
     3301 cgcgccagct ggacacgcaa gtcgcactga tccgatccct gggcggcggc tacgccgccg
     3361 gcgccgacat gcagacggcg caggcctcga actgaagcca caacgctgaa caacatctga
     3421 ccgttccgga gaatccgtca tgaacgcacc ccaacaagcc gtccccgtca atagcaacaa
     3481 cgaaaagcgc aagaaggcgc tcaccgccat cgccgccgtc gtcggcgtcg gggccatcgc
     3541 ctacggggcc tactacgccc tggtgctcaa ccactacgag agcaccgaca acgcctatgt
     3601 acaaggcaat gtcgt
//
LOCUS       DASUCY010000543         6131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_671302, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000543 DASUCY010000000
VERSION     DASUCY010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 6131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6131
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671302"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1038)
                     /locus_tag="VFV25_10705"
     CDS             complement(<1..1038)
                     /locus_tag="VFV25_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY1393839.1"
                     /translation="MPSLAAAPLARDADATLTEQLAARYAERIRQRLLAPGARLPSVR
                     ECARRHGVSTYTVVAAYDQLLAQGLIEARKQRGFFVRETQPPTRSRAAANEDRPPAPV
                     DAAALIRGMFQQPGSPLMPGFGTLPPDWLDLSMLSTALRRVTAGERLGPLALRYGEPA
                     GDSRLRAALAMKLDGFGLKAETSQIVTTIGATQALDIVSRTLLQPGDAVLVDEPGWAV
                     EFARLGRLGLRIVPVPRGAEGPDLAAMRSLIAAHKPRLYATVSVLHNPTGAMLSLAAA
                     HQVLKLAEVHDFHIVEDDTYAHLAPAHAPRLSALDGLQRTVYVSGFSKILTPAWRVGF
                     IAAPPALVERCI"
     gene            1207..2166
                     /locus_tag="VFV25_10710"
     CDS             1207..2166
                     /locus_tag="VFV25_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY1393840.1"
                     /translation="MAHEPAQSASAEQRRLRAGYLYGLAGVLLFALTIPMTRLAGGSV
                     ENPQLPPDFVAIGRAAVAGLLALAWLAATRAPLPRRELRGLLALSAAGAVFGFPLFLG
                     WAVRHVEAVHAAVITGFLPLATAAVAVLWLRQRASAAFWACATLGCALVAAYAWLKGG
                     GRFDAADGLLLLAVLSAALSYVGGAQLSVAGLSGPQVISWMLVLCLPVTLPLTLTLAV
                     GHWSQIGGQVHAAAWGGFAYVSVVSMWLGFFAWYRGLALGGTMKVSQVQLIQPFASML
                     AAVPLLGERLDFATIAFALAVIATVFIGKRVPVSRAKPDLQGD"
     gene            2172..3350
                     /locus_tag="VFV25_10715"
     CDS             2172..3350
                     /locus_tag="VFV25_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY1393841.1"
                     /translation="MNWTLARRAQRMNPSIIREILKLTEKPGVISLAGGLPAPETFPV
                     DAVREAVARVLTEAPQAALQYAASEGFGPLREWVAAQLNRQGMKVEASQVLITTGSQQ
                     GLDLCAKLMLDPGSRVAVETPTYLGALQAFTPYEPAVESLDSDAEGPTPEALSQARGA
                     RFAYLLPSFQNPSGRTMSEARRDAIVATAQAAGLPLLEDNPYGELWFDAAPPAPLAAR
                     WPEGVIYLGSFSKVLAPGLRLGYAVAPKPVFPKLLQAKQAADLHTPVFNQRVVHEVIK
                     DGFLDRHVPEIRARYKAQRDAMAAALQRYMPAGCHWQIPDGGMFFWLTLPRGMDAMAL
                     LPQAVEQGMAYVPGAAFFAGEGQPNTLRLSYVTVSAELIEKGIAALGRVLAGSEKLAA
                     "
     gene            3366..4202
                     /locus_tag="VFV25_10720"
     CDS             3366..4202
                     /locus_tag="VFV25_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEY1393842.1"
                     /translation="MSEHAFKQIDVFSDEPLKGNPLAVVFQADSLSEAQLAAFARWTN
                     LSETTFLLKPTTAEADYRVRIFTPGGELMFAGHPTLGSCHAWLQGGGQPRQPECVVQE
                     CGVGLVRVRRDGTRSAFAAPPLKLQPVSADELATAIAALGLAREQVREARWLDMGTRW
                     LGLLLGGADEVLAVKPRSGAMRDFAKVGLIGPHPEGASCQYEVRGFAEPIGVPEDPVT
                     GSLNAGLARWLIDAGIAPPRYVAAQGQALGRAGRVHVERIGADIWIGGHSVTRIEGRL
                     SL"
     gene            4219..4932
                     /locus_tag="VFV25_10725"
     CDS             4219..4932
                     /locus_tag="VFV25_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HEY1393843.1"
                     /translation="MTEELFREDAQLRTCEARITAVADGGVLLDRTVFYPQGGGQAGD
                     AGELLLANGSAIAIADTRKAAAPGEILHLLADPADAARLAPGQAVQARIDWQRRHRHM
                     RFHTATHLLCALVPHPVDGCSITAGYARLDFHMNEPLDKEALNTGLARLVAEAHAVRH
                     RWISDAELDANPQLVRSMSVQPPRGSGRVRLLEIEGVDLQPCGGTHVSSTAEIGPVIV
                     TKIEKKSAMTRRVVLGLAE"
     gene            5035..5547
                     /locus_tag="VFV25_10730"
     CDS             5035..5547
                     /locus_tag="VFV25_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY1393844.1"
                     /translation="MYKHQVDIQFTSPTTPTALAAVRELMREYARSLDVDLCFQDFER
                     ELAGLPGDYAPPGGLLLLAHVDGELAGCGAFRPLANSEHLAACEMKRLYLRPDFRGLQ
                     LGRRLAQALVQRAREAGYASMLLDTLSDMKAARGLYAELGFRPVPPYYDNPLPGVLYM
                     KLDLGPAVAT"
     gene            5701..>6131
                     /locus_tag="VFV25_10735"
     CDS             5701..>6131
                     /locus_tag="VFV25_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEY1393845.1"
                     /translation="MNAFRHLLKAAGAHPPVGTWIMSASPLVAEAVGHAGFDWGVIDM
                     EHSPLDMMGVIHLLQAVAGTKMIPVVRVPWNDAVVIKRVLDAGATTLLVPFVQNAEEA
                     RRAVAATRYPPEGLRGMAGMSRASRYGLQSNYAQHANEGMGV"
BASE COUNT          877 a         2136 c         2159 g          959 t
ORIGIN      
        1 gatgcagcgc tccaccaggg ccggcggcgc ggcgatgaag cccacgcgcc aggccggcgt
       61 gaggatcttg gaaaagcccg agacgtagac ggtgcgctgc agcccgtcca gcgccgacag
      121 ccgcggcgca tgtgcgggtg ccaggtgggc ataggtgtcg tcctcgacga tgtggaagtc
      181 gtgcacctcg gccagcttca gcacctggtg cgctgcggcg agcgagagca tcgcgccggt
      241 tgggttgtgc agcaccgata cggtggcgta gaggcgcggc ttgtgcgcgg cgatgagcga
      301 gcgcatcgct gccaggtcgg gcccctcggc gccgcgcggc accggcacga tgcgcaggcc
      361 caggcggccc aggcgggcga actccaccgc ccagccgggc tcgtcgacca gcaccgcgtc
      421 gcccggctgc agcagcgtgc gcgagacgat gtccagcgcc tgggtagcgc ctatcgtcgt
      481 cacgatctgc gaggtctccg ccttcaggcc gaagccgtcc agcttcatcg ccagggccgc
      541 gcgcagccgg gagtcgccgg ccggctcgcc gtagcgcagc gccagcgggc ccaggcgctc
      601 gcccgctgtc acgcggcgca gcgccgtgct gagcatcgac aaatccagcc aatcaggcgg
      661 cagcgtgccg aagcctggca tgagtgggct gccgggctgc tggaacatgc cgcgtatcag
      721 cgctgcggcg tccaccggcg caggcgggcg atcctcgttc gcggcggcgc ggctgcgcgt
      781 tggcggctgg gtctcgcgca cgaagaagcc gcgctgcttg cgcgcctcga tcaagccttg
      841 ggccagcagt tggtcatagg ccgcgaccac cgtgtaggtg ctgacgccgt ggcgcctggc
      901 gcattcgcgc accgaaggca ggcgcgcgcc cggtgccagc aggcgctgac gtatgcgttc
      961 ggcgtagcgc gcggcgagct gctcggtgag cgtcgcgtcg gcgtcacggg caagcggtgc
     1021 tgcggcgagg ctgggcatgg cagggatgcg atgtgttgga gcggtgaccg atacagcaca
     1081 aagactgatc aggcgcagtg tattggttct gtgttggtcg agtccataca ctagctcatg
     1141 ctcggcggcc ggtcgagcca aggccccttt tcactcatgt atcggaccgc ttcccgcccg
     1201 ccattggtgg cgcatgaacc cgcgcagagc gccagcgcgg agcagcggcg tcttcgcgcg
     1261 ggctatctct acgggctggc tggcgtgctg ctgttcgcgc tgacgattcc gatgacgcgc
     1321 ctggccggcg gcagcgtcga gaacccgcaa ctaccgcccg atttcgttgc catcggccgt
     1381 gccgccgtcg cggggctgct ggcgctggcc tggctcgccg ccacgcgcgc gccgttaccg
     1441 cgccgcgagc tccggggcct gctggccttg agcgcggccg gtgccgtgtt cggctttccg
     1501 ctcttcctcg gctgggccgt gcgccatgtc gaggcggtgc atgcggccgt catcaccggc
     1561 ttcctgccgc tggccaccgc cgcggttgcg gtgctttggc tcaggcagcg cgcttcggcc
     1621 gccttctggg cctgcgccac gctcggctgc gcgctggtcg ccgcttatgc ctggctcaag
     1681 ggcggcgggc gtttcgacgc ggccgatggc ttgctgctgc tggcggtgct gagcgccgcg
     1741 ctcagctatg tcggcggcgc ccagctcagc gttgccggcc tgagcgggcc gcaggtgatc
     1801 tcctggatgc tggtgctgtg cctgccggtc accttgccgc tgacgctcac gctcgccgtc
     1861 gggcattgga gccagatcgg cggtcaggtg cacgcggccg cctggggcgg cttcgcctat
     1921 gtgtccgtcg tttcgatgtg gctgggcttc ttcgcctggt accgcggcct ggcactgggc
     1981 ggcaccatga aggtcagcca ggtgcagctg atccagccct tcgcctcgat gctggcggcc
     2041 gtgccgctgc tgggcgagcg cctcgacttc gcgaccatcg ccttcgcgct ggccgtcatc
     2101 gccaccgtct tcatcggcaa gcgcgtgccg gtgagccgag caaaacccga tctgcaagga
     2161 gactaaccag catgaactgg accctggcgc gccgcgccca acgcatgaat ccctcgatca
     2221 tccgcgagat cctcaagctc accgagaagc ccggcgtgat ctcgctggcc ggcggcctgc
     2281 cggcgcccga gaccttcccg gtcgatgcgg tgcgcgaggc ggtcgcccgc gtactgaccg
     2341 aggctccgca ggccgcgctg caatacgccg ccagtgaagg ctttggtcct ttgcgcgaat
     2401 gggtcgccgc gcagctgaac cggcagggca tgaaggtcga ggccagccag gtgctgatca
     2461 cgaccggctc gcagcagggc ctggaccttt gcgccaagct gatgctggac cccggctcgc
     2521 gcgtcgcggt ggagacgccc acttatctcg gcgcgctgca ggccttcacg ccctatgagc
     2581 cggcggtcga gtcgctggat tccgacgccg agggcccgac acccgaggcc ttgagccaag
     2641 cccgcggcgc gcgcttcgcc tatttgctgc cgagcttcca gaacccgagc ggccgcacga
     2701 tgagcgaggc gcgccgcgac gcgatcgttg ccaccgccca ggcggccggc ctgccgctgc
     2761 tcgaagacaa cccctacggc gagctgtggt tcgacgccgc gccgcccgcg ccgctggccg
     2821 cgcgctggcc cgaaggcgtg atctacctcg gctccttctc caaggtgctc gcgccgggcc
     2881 tgcgcctggg ctatgcggtg gcgcccaagc ccgtcttccc taagctgctg caggccaagc
     2941 aggcggccga cctacacacg ccggtgttca accagcgcgt cgtgcacgag gtcatcaagg
     3001 atggctttct ggatcgccac gtgcccgaga tccgcgcgcg ctacaaggcc cagcgcgatg
     3061 cgatggcggc ggcgctgcag cggtacatgc cggccggctg ccactggcag atcccggatg
     3121 gcggcatgtt cttctggctc acgctgccgc gcggcatgga tgccatggcg ctgctgcccc
     3181 aggccgtgga gcagggcatg gcctatgtgc caggcgcggc cttcttcgcc ggtgagggcc
     3241 agcccaacac gctgcggctg agctatgtca ccgtgtcggc cgagctgata gaaaagggca
     3301 tcgcggcgct gggccgcgtg ttggcaggca gcgaaaaact ggctgcatga agcaagggca
     3361 acacgatgag tgagcacgcc ttcaagcaga tcgatgtctt cagcgatgag ccgctgaagg
     3421 gcaacccgct ggccgtcgtg ttccaggccg acagcctgag cgaagcgcag ctggccgcct
     3481 tcgcgcgctg gaccaatctg tctgagacca cttttctgct gaagccgacc acggccgagg
     3541 ccgactaccg cgtacgcatc ttcacgcccg gcggcgagct gatgtttgcg ggccacccca
     3601 cgctcggcag ctgccatgcc tggctgcagg gcggcgggca gccgcgacag cctgagtgtg
     3661 tggtgcagga gtgcggcgtg ggcctggtgc gcgtgcgtcg cgatggcact cgctcggcct
     3721 ttgcggcgcc gccgctgaag ctgcagcctg tctcggccga cgagctggcg accgccatcg
     3781 ccgcgctcgg ccttgcccgc gagcaggtgc gcgaggcccg ctggctggac atgggcacgc
     3841 gctggctggg gctgctgctc ggcggcgcgg acgaggtgct ggccgtgaag ccgcgcagcg
     3901 gcgcgatgcg ggacttcgcc aaggtcggcc tgatcggccc ccatcccgag ggtgcgtcct
     3961 gccaatacga agtgcgtggc ttcgccgagc cgatcggcgt gcccgaagat ccggtgaccg
     4021 gcagcctcaa cgccggcctg gcgcgctggc tgatcgacgc gggaatcgcg cccccgcgct
     4081 atgtggcggc ccagggccag gcgctgggcc gcgccggccg cgtgcacgtg gagcgcatcg
     4141 gcgcagacat ctggattggc gggcacagcg tgacccgcat cgaaggccga ctcagcctct
     4201 aaccgccgcg gagccgtcat gaccgaagaa ctcttccgcg aggacgcgca gctgcgcacc
     4261 tgcgaggcgc gcatcacggc cgtggccgat ggcggcgtgc tgctggaccg cacggtcttc
     4321 tatccgcaag gcggcggcca ggccggcgac gccggcgagc tgctgctggc aaacggtagc
     4381 gcaattgcga tcgccgacac gcgcaaggcg gcagcgcccg gcgagatcct gcacctgctg
     4441 gccgacccgg ccgatgccgc aaggctcgcg cccggccaag cggtgcaggc ccgcatcgac
     4501 tggcagcgcc ggcatcgcca catgcgtttc cataccgcga cgcatctgct gtgcgcgcta
     4561 gtgccgcatc cggtcgacgg ctgctccatc acggcgggct atgcgcggct cgatttccac
     4621 atgaacgagc cgctggacaa ggaggcactc aacaccggcc tggcccgcct cgtggccgag
     4681 gcccatgcgg tgcgccaccg ctggatcagc gatgccgagc tcgacgccaa cccccagctc
     4741 gtgcgcagca tgagcgtgca gccgccgcgc ggcagcggcc gcgtgcgcct gctggagatc
     4801 gagggcgtcg atctgcagcc ctgcggcggc acgcatgtaa gctcgaccgc cgagatcggc
     4861 cccgtcatcg tcacgaagat agagaaaaaa agcgcgatga cccgcagggt cgtgctcggc
     4921 cttgcggagt aaacctgcgc ggcgcggcgc cgtgaagcca gtccgggcac agctggccgc
     4981 cggtccggac gaatgactac actgccatta cgtccgtgac agccgcagac gggcatgtac
     5041 aagcatcagg tcgacatcca attcacgagt cccacgacgc ccacggccct ggccgccgtg
     5101 cgcgaactca tgcgcgagta tgcgcgctca ctggacgtgg acttgtgctt ccaggacttc
     5161 gagcgcgagc tcgccgggct gccaggcgac tacgcgccgc ctggaggcct cctgctgctg
     5221 gctcatgtcg atggcgagct ggctggctgc ggcgctttcc gtccgcttgc gaattccgag
     5281 cacctggcgg cctgcgagat gaagcgcctc tatctgcgcc ccgactttcg cggcctgcag
     5341 ctgggccgcc ggctggcaca ggcccttgtg caacgcgccc gcgaggcggg ctacgcgtcg
     5401 atgctgctcg acacgctgtc cgacatgaag gcggcgcgcg gcctttacgc ggagctgggc
     5461 ttcaggccgg tgccgcccta ctacgacaat ccgctgccgg gcgtgctcta catgaagctc
     5521 gacctcggtc cagccgtcgc gacgtaacat gcactcgctc catcatgaat cgcgcaattc
     5581 ctgcgctggt gtgacccagc agacgccgcg gatccggctt tgccggttcg ctggcgtcgc
     5641 ccccttgagg gggtgcgccg aaggcgctcc gggggtgggg ctaccaacag gagacccgcg
     5701 atgaatgcat tcagacatct gctgaaggcc gccggcgccc atccgccggt gggcacctgg
     5761 atcatgtcgg ccagcccgct ggtcgccgag gccgtgggcc acgccggctt cgactggggc
     5821 gtgatcgaca tggagcattc gccgctggac atgatgggcg tgatccacct gctgcaggcg
     5881 gtggccggca ccaagatgat cccggtggtg cgcgtgccct ggaatgatgc ggtcgtcatc
     5941 aagcgcgtgc tcgatgccgg cgccaccacg ctcctggtgc ccttcgtgca aaacgccgag
     6001 gaggcgcgcc gcgcggttgc agccacacgc tatccgcccg aaggcctgcg cggcatggcc
     6061 ggcatgagcc gggcctcgcg ctacggcctg cagtcgaact acgcccagca cgccaacgag
     6121 ggcatgggcg t
//
LOCUS       DASUCY010000544         2458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1570576, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000544 DASUCY010000000
VERSION     DASUCY010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1570576"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(256..2124)
                     /gene="glmS"
                     /locus_tag="VFV25_10740"
     CDS             complement(256..2124)
                     /gene="glmS"
                     /locus_tag="VFV25_10740"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEY1393846.1"
                     /translation="MCGIVGAVSQRNIVPILIEGLKRLEYRGYDSCGVAVHQDGALRR
                     ARSTSRVAELDAQVEHEHVIGGTGIAHTRWATHGAPAVHNAHPHFSHGSGADAANAKP
                     GRIALVHNGIIENHDELRAELKARGYVFNSQTDTEVIAHLVDQHYEGDLFEAVKRATR
                     ELKGAYAIAVFCRDEPHRVVGAREGSPLVLGVKADSGENFLASDAMALAGVTDQIVYL
                     EEGDVVDLQLGKYWIESRQGGERFVRVERPVRTVHAHTGAAELGPYRHYMQKEIFEQP
                     RAVADTLEGVHSVTPELFGDGAYRIFKDVEQVLILACGTSYYSGSTAKYWLESIAKIP
                     TTVEIASEYRYRDSVPNPKTLVVTISQSGETADTLAALKHARALGMPHTLTICNVATS
                     AMVRECELAYITRAGVEIGVASTKAFTTQLAGLFLLTLALAQVRGQLSDAQEAEHLKA
                     MRHLPLALQAVLALEPQVIGWAEAFARKENALFLGRGLHYPIALEGALKLKEISYIHA
                     EAYPAGELKHGPLALVTSEMPVVTVAPNDTLLEKLKSNMQEVRARGGELFVFADADTR
                     IESGEGLHVIRMPEHYGALSPILHVVPLQLLAYHTACARGTDVDKPRNLAKSVTVE"
     gene            2280..>2458
                     /locus_tag="VFV25_10745"
     CDS             2280..>2458
                     /locus_tag="VFV25_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013390992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY1393847.1"
                     /translation="MTSPQLDALDWRILDQLQQDASLSNQALAERVHVSPATCLRRVK
                     RLVDSGVIEKRIAILS"
BASE COUNT          404 a          771 c          825 g          458 t
ORIGIN      
        1 agaccgcgcc tgaggaggct cggcatggcc ttaaacaatc ggcggcaatg gccagccgca
       61 ctcgcctagc gagtgcggcg ctgctaggtg ccctgcagag cagcggcgca gcagctggga
      121 tatggtggcc ggcggtcatt cgaatcgcgt gtcgtcacca tgcgaatccc acatggtgaa
      181 aacatcccga cggaccttat ttggttccga cggactttat ttggtgcaat catcttcctc
      241 cggagcccac caccgtcact caacagtaac cgacttcgcg aggttacgag gcttatccac
      301 gtcggtcccg cgcgcgcagg ccgtgtgata ggccagtagc tgcagcggca ccacgtgcag
      361 gatgggcgag agcgcgccgt agtgctcggg catgcggatc acgtgcaggc cttcgccgct
      421 ctcgatgcgg gtgtcggcat cggcaaagac gaagagttcg ccgccgcggg cgcgcacttc
      481 ctgcatgttg ctcttgagct tttcgagcag ggtgtcgttg ggcgccacgg tgacgaccgg
      541 catctcgctc gtcaccagcg ccagcggccc gtgcttgagc tcgccggccg ggtaggcctc
      601 ggcgtggatg tagctgatct ccttgagctt taaggcacct tcgagcgcga tggggtagtg
      661 caggccgcgc ccaaggaaga gcgcgttctc cttgcgcgca aaggcctcgg cccagccgat
      721 cacttggggc tccagcgcga gcacggcctg cagcgcgagc ggcaggtggc gcatggcctt
      781 gaggtgttcg gcctcctgcg catcgctcag ctgcccgcgc acctgggcca gggccagcgt
      841 gagcaggaag aggccggcga gctgggtcgt gaaggccttg gttgaagcga cgccgatctc
      901 cacgcctgcg cgcgtgatgt aggcgagctc gcattcgcgc accatcgcgc tggtggcgac
      961 gttgcagatg gtcagcgtgt ggggcatgcc cagcgcgcgc gcatgcttga gggcggccag
     1021 ggtgtcggcc gtttcgccgc tctggctgat cgtgacgacc agggtcttgg ggttgggcac
     1081 gctgtcgcgg tagcggtatt cgctggcgat ttccacagtg gtcgggatct tggcgatgct
     1141 ttccagccag tacttggcgg tcgagcccga gtagtagctg gtgccgcagg ccaggatcag
     1201 cacctgctcc acgtccttga agatgcggta ggcgccgtcg ccgaagagct cgggcgtcac
     1261 gctatgcacg ccttccagcg tgtcggcaac ggcacgcggc tgctcgaaga tctctttttg
     1321 catgtagtgg cgatagggcc cgagctcggc cgcgccggtg tgggcgtgca cggtgcgcac
     1381 aggacgctcg acgcggacga agcgctcgcc gccctggcgc gactcgatcc agtatttgcc
     1441 cagctgcagg tcgaccacgt cgccttcttc gaggtagacg atctggtcgg tcacgccggc
     1501 cagcgccatc gcatcggagg ccaggaagtt ctcgccgctg tcggccttga cgcccagcac
     1561 cagcggcgag ccttcgcggg ccccgacgac gcggtgcggc tcgtcgcggc agaacacggc
     1621 gatcgcatag gcgcccttga gctcgcgcgt tgcgcgcttg accgcctcga agagatcgcc
     1681 ttcgtaatgc tggtcgacca gatgcgcgat gacttcggtg tcggtctggc tgttgaagac
     1741 atagccgcgc gccttgagct cggcgcgcaa ctcgtcgtgg ttctcgatga tgccgttgtg
     1801 caccagcgcg atgcggccgg gcttggcatt ggcggcatcg gccccgctac cgtgcgagaa
     1861 atgcgggtgg gcgttgtgca cggccggcgc accatgcgtg gcccagcgcg tgtgggcgat
     1921 accagtcccg ccgatcacat gctcatgctc gacctgggcg tcgagctcgg ccacgcgcga
     1981 ggtgctgcgg gcgcggcgca gggcgccgtc ctgatgcacg gccacgccgc aggagtcgta
     2041 gccgcggtat tcgagacgct ttaggccctc gatcaggatg ggaacgatgt tgcgctggct
     2101 gacagcgccg acgatgccgc acatggaaga agtctccggc cgagcttgga atgccaggat
     2161 ggtagacagg gctgcatgaa attaaattgc aatctatcat ctgatgcgcg ctattcgatc
     2221 ttcattcatc attgccgatc caaaatttct aaatacagaa attcttgaaa taataagcca
     2281 tgacctcccc gcaactggat gccctcgatt ggcgcatcct cgaccagctg cagcaagacg
     2341 cctcgctgag caaccaggcc ctggccgaac gcgttcacgt ctcgccggcc acctgcctgc
     2401 ggcgcgtcaa gcggctggtg gactccggcg tgatcgagaa gcgcatcgcc atcctctc
//
LOCUS       DASUCY010000545         2517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1490161, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000545 DASUCY010000000
VERSION     DASUCY010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1490161"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VFV25_10750"
     CDS             <1..288
                     /locus_tag="VFV25_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEY1393848.1"
                     /translation="APQKTLFPVAGRNAIATIKGQLVNMRDGGFISAHDFHIASLIAD
                     VVCGGDVDAGSLVSEEYLMALERKHFCGLLDHPKTQERIMGMLQTGKPVRN"
     gene            316..1512
                     /locus_tag="VFV25_10755"
     CDS             316..1512
                     /locus_tag="VFV25_10755"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEY1393849.1"
                     /translation="MSKQVQDAYIVAATRTPIGRSGRGYFKNTRPDDLLVAAVKSALA
                     QVPSLDTKAIEDAIIGCSFPEGEQGMNMARIAMALAFPHPVGGVTVNRFCASGLTAIQ
                     MAADRIRVGEADVLIAGGAESMSLVPMGGNKPSFNPEVFAKDENVGIAYGMGLTAEKV
                     AQQWKVSREAQDAFAYESHMRALKAIAAGEFKDEITPIEVVARFPSLETGEQGVKTRA
                     VDIDEGPRPDTSLEGLAKLKPVFAAKGSVTAGNSSQTSDGAGALILASEKAIKQFDLK
                     PLARFVSFAARGVPPEIMGIGPIEAIPAALKYAGLTQDQIDWIELNEAFAAQSLAVIN
                     SVGLDPKKVNPMGGAIALGHPLGATGAIRSATVVHALRRHNLKYGMVTMCVGTGQGAA
                     GIFERV"
     gene            1585..2355
                     /locus_tag="VFV25_10760"
     CDS             1585..2355
                     /locus_tag="VFV25_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEY1393850.1"
                     /translation="MSDILVHLEASVLTLTFNRLDKKNAITQAMYAALADALTEAPGD
                     DKVRAVVIQGHEQIFTAGNDLQDFLSAPPSAEAREAAPVFRFLHTIQAFPKPIVAAVC
                     GAAVGIGTTLLLHCDLVYAGDNAAFSLPFVNLGLCPEAGSSFLLPQLLGYQRAAEKLL
                     LGEPFYAAEAAEIGIVNRVLPPREVNTYAQAQAAKLAAKPSASLKAAKALMKKSAQEA
                     AARMDEEGEIFARMLREPAAREAMTAFMEKRKPDFSQW"
     gene            2424..>2517
                     /locus_tag="VFV25_10765"
     CDS             2424..>2517
                     /locus_tag="VFV25_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010110572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short chain dehydrogenase"
                     /protein_id="HEY1393851.1"
                     /translation="MNLKGKTLFITGASRGIGLAIAKRAAADGAN"
BASE COUNT          463 a          871 c          809 g          374 t
ORIGIN      
        1 gcgccgcaga agacgctgtt cccggtggcc ggccgcaacg cgatcgccac catcaagggc
       61 cagctcgtca acatgcgcga cggcggcttc atcagcgcgc acgacttcca catcgcctcg
      121 ctgatcgccg acgtggtctg cggcggcgat gtcgatgccg gttcgctggt cagcgaggaa
      181 tacctgatgg cgctggagcg caagcatttc tgcggcctgc tggatcaccc caagacccag
      241 gaacggatca tgggcatgct gcaaacgggc aagcctgtcc gcaactgatc ggccggcacc
      301 tcaaggaaac acatcatgag caagcaagtt caagacgcct acatcgttgc cgccacgcgc
      361 acgccgatcg gccgctcggg ccgcggctat ttcaagaaca cccggcccga cgacctgctg
      421 gtcgccgccg tcaagagcgc gctggcgcag gtgccctcgc tggacaccaa ggccatcgag
      481 gacgccatca tcggctgctc cttccccgag ggtgagcagg gcatgaacat ggcgcgaatc
      541 gcgatggcgc tggccttccc gcatccagtg ggcggcgtca cggtcaatcg cttctgcgcc
      601 tcgggcctca ccgcgatcca gatggcggcc gaccgcatcc gcgtcggcga ggccgacgtg
      661 ctgatcgccg gcggcgccga gtcgatgagc ctggtgccga tgggcggcaa caagccctcg
      721 ttcaaccccg aggtgttcgc caaggacgag aacgtcggca tcgcctacgg catgggcctg
      781 accgccgaga aggtggctca gcaatggaag gtcagccgcg aggcgcagga cgccttcgcc
      841 tacgagtcgc acatgcgcgc gctcaaggcg atcgctgccg gcgagttcaa ggacgagatc
      901 acgccgatcg aggtggtcgc gcgcttcccc agcctcgaaa ccggcgagca gggcgtcaag
      961 acgcgcgcgg tcgacatcga cgagggcccg cgccccgaca cctcgctcga aggcctggcc
     1021 aagctgaagc cggtgttcgc cgccaagggt agcgtcaccg ccggcaacag ctcgcagacc
     1081 agcgacggcg ccggcgcgct gatcctggcc agcgaaaagg cgatcaaaca gttcgacctc
     1141 aagccgctgg cccgcttcgt cagcttcgcc gcccgcggcg tgccgcccga aatcatgggc
     1201 atcggcccga tcgaggcgat cccggcggcg ctcaagtacg ccggcctaac gcaggaccag
     1261 atcgactgga tcgagctgaa cgaggccttt gccgcgcagt cattggccgt gatcaacagc
     1321 gtcggcctcg acccaaagaa ggtgaacccg atgggcggcg ccatcgcgct cggccacccg
     1381 ctgggcgcca ccggcgcgat ccgctcggcc accgtggtgc atgcgctgcg ccgccacaac
     1441 ctgaagtacg gcatggtgac gatgtgcgtc ggcaccggcc agggcgcggc gggtatcttc
     1501 gagcgcgtct gaagctacga cagcaagccc gatacctggc gacaagctgg gcgcgggctt
     1561 tttctcatta cgctggagac aagcatgtcc gacatcctcg tgcacctcga agcctcggtg
     1621 ctcacgctca ctttcaaccg cctcgacaag aagaacgcca tcacgcaggc gatgtacgcc
     1681 gcgctggccg atgcgctgac cgaggctccc ggcgacgaca aggtgcgcgc ggtggtgatc
     1741 cagggtcacg agcagatctt caccgctggc aacgacctgc aggacttcct ctccgcgccg
     1801 ccatcggcag aggcacgcga ggcagcgccg gtgttccgct tcctgcacac catccaggcc
     1861 ttccccaagc ccatcgtcgc ggcggtgtgc ggcgcggcgg tgggcatcgg caccacgctg
     1921 ctgctgcatt gcgatctcgt ctacgctggc gacaacgcgg ccttcagcct gcctttcgtg
     1981 aacctggggc tgtgcccaga ggccggctcc agcttcctgt tgccgcagct gctgggctac
     2041 cagcgcgcag ccgaaaagct gctgctgggc gagcctttct acgctgccga ggcggccgag
     2101 atcggcatcg tgaaccgcgt gttgccgccg cgcgaggtga atacctatgc tcaggcgcag
     2161 gcagccaaac tggccgccaa gccttcggcc tcgctcaagg ccgccaaggc cttgatgaag
     2221 aaatccgcgc aggaggccgc cgcccgcatg gacgaggagg gcgagatctt cgcccgcatg
     2281 ctgcgcgagc cggcggcacg cgaggcgatg accgccttca tggagaagcg caagcccgac
     2341 ttctctcaat ggtgacctgt gactcgtgac tagtgaccag tcacgagtca ccagtcacga
     2401 gtcactgttc gctaggagaa gaaatgaatc tcaaaggcaa gaccctcttc atcaccggcg
     2461 catcgcgcgg catcggcctg gccatcgcca agcgcgcggc ggcggacggc gccaaca
//
LOCUS       DASUCY010000546         2646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1554801, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000546 DASUCY010000000
VERSION     DASUCY010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1554801"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1120
                     /locus_tag="VFV25_10770"
     CDS             <1..1120
                     /locus_tag="VFV25_10770"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEY1393852.1"
                     /translation="CASCHDPARAFSSDNGGRDGGAQGSVRGRLGTRNPPSLTYVRYV
                     PPLYFYKDDDAPSPQPFGGLFADGRVDTLARQAREPLLAPHEMNNGSGAAAARQLQRT
                     GYRAAFEAQFGPGVLQQPRRAVQAFGEAIAAYLGSDELSPFNSRFDEMLRGKPALTPQ
                     ELRGLALFKDPDRGNCASCHSVNDRSSNPARSLFTDFGYDALGVPRNRALPMNRDPRS
                     YDLGLCITARQRHWPDGDSWCGYFRTPSLRNVAVRKRYMHNGVFTSLREAVAFYATRS
                     TQPADWYRGGELFDDLPAAMRGNVNIVSTPLNRRAGSTPALSEDDIDALVAFLKTLTD
                     VRYAPLVEAAPPAPAATATSPAARCPPAPAPCRRPSRT"
     gene            complement(1050..1976)
                     /locus_tag="VFV25_10775"
     CDS             complement(1050..1976)
                     /locus_tag="VFV25_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY1393853.1"
                     /translation="MSSSATLATPRASSSRSPVWPGLALAMAGSIAFSGKAIIVKLAY
                     RYGVDAVTLIFYRMLFAAPLFALIAWWSGRGKPPLLRRDWLGILGLGVSGYYLASFLD
                     FLGLQYITANLERLILYLNPTLVLALSILLFKKQVSGRQLVAIGVSYAGVLLVFGHEL
                     GFQHARNVPLGAVLVFASAVSYAIYLVYSGELVQRLGSLRLVGWASLVACAFCIGQFL
                     VLRPLSAAAVAPQVIWLSVLNATACTVAPVLMVMMAIERIGATLAAQTGMIGPLSTIT
                     MSALILGEPFTSWTAAGTVLVLAGIWLLAKWR"
     gene            complement(2113..2247)
                     /locus_tag="VFV25_10780"
     CDS             complement(2113..2247)
                     /locus_tag="VFV25_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393854.1"
                     /translation="MHEWDTIEAFMDEVRMARIYDGVHYRNSTLVGNQIGTAVGALVQ
                     "
BASE COUNT          490 a          938 c          844 g          374 t
ORIGIN      
        1 ctgcgccagc tgccacgacc cagcccgagc cttcagcagc gacaacggcg ggcgcgacgg
       61 cggggcgcag ggcagcgtgc gtgggcgtct ggggacgcgc aacccgcctt cgctgaccta
      121 tgtgcgctac gtgccgccgc tctacttcta caaggacgac gacgcgccct cgccccagcc
      181 cttcggcggc ctgttcgccg atggccgcgt agacacattg gcccggcagg cgcgcgagcc
      241 gctgctggcg ccgcacgaga tgaacaacgg cagcggagca gcggccgcgc gccagttgca
      301 gcgcaccggc tatcgcgcag ccttcgaggc gcagttcggc cccggcgtgc tccagcagcc
      361 ccggcgcgcc gtgcaggcct tcggcgaggc gatcgccgcc tacctcggca gcgacgagct
      421 ctcgcccttc aactcgcgct tcgacgagat gctgcgcggc aagcctgcgc tcacgccgca
      481 ggagctgcgc ggcctggcgc tgttcaagga tccggaccgc ggcaattgcg cctcctgcca
      541 cagcgtcaac gaccgatcca gcaacccggc gcgctcgctc ttcacggatt tcggctacga
      601 cgccctgggc gtgccgcgca accgcgcgct gccgatgaac cgcgacccgc gcagctacga
      661 cctcgggctg tgcatcaccg cgcggcagcg ccactggccg gacggcgaca gctggtgcgg
      721 ctacttccgc acgcccagcc tgcgcaacgt ggcggtgcgc aagcgctaca tgcacaacgg
      781 cgtattcacg tcgctgcgtg aagccgtggc cttctacgcc acgcgctcga cgcagccggc
      841 cgactggtac cgcggcggcg agctcttcga cgatctgccc gcggcgatgc gcggcaatgt
      901 caacatcgtg tccaccccgc tcaaccgccg cgccggcagc acgccggcgc tgagcgagga
      961 cgacatcgat gcgctggtag ccttcctgaa gacgctgacc gatgtgcgct atgcaccttt
     1021 ggtcgaggcc gcgccgcctg cgccagcggc taccgccact tcgccagcag ccagatgccc
     1081 gccagcacca gcaccgtgcc ggcggccgtc caggacgtga agggctcgcc gaggatcagc
     1141 gcgctcatcg tgatggtgga caaggggccg atcatgccgg tctgcgccgc cagcgtggcg
     1201 ccgatgcgct cgatcgccat catcaccatc aacaccggcg ccaccgtgca ggctgtcgcg
     1261 ttcagcaccg acagccagat cacctgcggc gcgacggccg ccgccgagag cgggcgcagc
     1321 acgaggaact ggccgatgca gaaggcacaa gccacgaggc tggcccagcc gacgagccgc
     1381 agcgacccca gccgctgcac cagctcgccg ctgtagacga ggtagatggc gtaggagacg
     1441 gcactggcaa agaccagcac ggctcccagc ggcacattgc gcgcatgctg gaagcccagc
     1501 tcatggccga acacgagcag cacgccggca taactcacgc cgatggcgac gagctgcctg
     1561 ccgctgacct gcttcttgaa cagcaggatg ctcagcgcca gcaccagcgt cgggttgaga
     1621 tagaggatca gccgctccag gttcgcggtg atgtactgca agccgaggaa atcgagaaaa
     1681 ctcgccaggt agtagccgct cacgcccagg cccaggatgc ccagccagtc gcgccgcaaa
     1741 agcggcggct tgccgcgccc cgaccaccac gcgatcagcg cgaacaaggg cgcggcgaac
     1801 agcatccggt agaagatcag cgtcaccgcg tcgacgccgt agcgataggc cagcttgacg
     1861 atgatggcct tgcccgagaa ggcgatcgag cctgccatcg cgagcgccag gccgggccac
     1921 actggcgagc gggaagaaga cgcgcgcggc gtcgcgaggg tagcgctgct gctcatcggc
     1981 cgattctagg aagcctgcaa agacctgtgc cggcccgggc ttcgcgagtc gactagcccc
     2041 cttgctccgc cgcgctcctc tcacggcatg tactgctccc ctttggcgac tagcgaccgg
     2101 ttgagcgaaa cgctactgca ccaatgcccc cacggccgtg ccgatctggt tgcccacaag
     2161 cgtcgagttc cggtagtgca cgccatcgta gatgcgcgcc atgcgcactt cgtccatgaa
     2221 ggcttcgatc gtgtcccact cgtgcaaccc ctcacgtagg ggcccgcgaa cacggaggcg
     2281 aagctcaagg gaaatgcatc agcggtgacc gctcagatag gagcccaccg cacatgttgg
     2341 atatctgcac aagtgtcaaa aggtcacgaa acaccgagcg ttgcgaaatc cacaaagcac
     2401 ggtgtcaatc cgcatgcacc ttcaatagac tgcgaacttt ttcacgctta gagcctagcc
     2461 aactaggcaa gggaggaggg aaccatgcgg atcctgctga tcgccagcgc gttcaacagc
     2521 ctgactcaac gcgtctttgc agaactgggt gaccacgggc acagcgtcgc gcttgtgctg
     2581 gccttgggcg acgacgaagc cttgcgcgag gcggttcgcc gccatcggcc tgagctgatc
     2641 gtggca
//
LOCUS       DASUCY010000547         2208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_735286, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000547 DASUCY010000000
VERSION     DASUCY010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735286"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VFV25_10785"
     CDS             <1..579
                     /locus_tag="VFV25_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HEY1393855.1"
                     /translation="RMTVNRALRELAAEGYVERVQGVGTFAAQLHRISSTLTIRDLHE
                     EIEQRGHQHRAEVQLVRAEPAPAELATRLGLAEGDEVFHSLIVHRENGVAIQCEDRYV
                     NPACAPGYLSVDFTRTTPTQYLLQVAPLGEAEYSIEASLPTAREAKLLGIERGEPCLV
                     VVRRTTSRGRPVTLARLVHPGTRYCLEGQFKP"
     gene            584..1183
                     /locus_tag="VFV25_10790"
     CDS             584..1183
                     /locus_tag="VFV25_10790"
                     /inference="COORDINATES: protein motif:HMM:NF017747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HEY1393856.1"
                     /translation="MSGPQLIRVDAIKPEPWRNGGGSTRTLLAWPSAEHWDLRISVAE
                     IAMDGPFSAYPDIERWFAVIEGAGVVLSSEGSNLALDPDSPPHRFDGASAPQCRLVDG
                     ATRDLNLMLRQDRLSGSLERAGSGVRWQSTAQLQGLYADDAGRLSDDAGRQWPLPART
                     LCWWQAAAGEPRSWRFESAAAHPLRAWWWQAAPTKEASR"
     gene            1180..>2208
                     /gene="hutI"
                     /locus_tag="VFV25_10795"
     CDS             1180..>2208
                     /gene="hutI"
                     /locus_tag="VFV25_10795"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEY1393857.1"
                     /translation="MSLTLWRHAHLATLDGRAPYGWLEHGAMLSEGETLRWVGAEADL
                     PATLARQVTVEHDLGGACVSPGLIDCHTHLVYGGQRAQEFELRLQGASYEEIARAGGG
                     IRSTVAATRAASDAQLLDSATRRLQALCAEGLTTIEIKSGYGLSAEHEARCLRIARRL
                     GEKLPLSVRTTYLAAHALPPEFEGRADDYIDAVCAWLPQLHAQGLVDAVDAFCERIGF
                     TPAQTRRVFDAARRLALPVKLHAEQLSDQGGAALAAEFGALSCDHLEYLGETGVQAMA
                     RAGTVAVLLPGAYYFLRETQLPPVAALRRHGVPIAIASDHNPGSSPALSLLLMLNMAC
                     TLFRLTPEE"
BASE COUNT          343 a          788 c          761 g          316 t
ORIGIN      
        1 cgcatgaccg tgaaccgtgc gctgcgcgag ctggcggccg agggctatgt cgagcgcgtg
       61 cagggcgtgg gcactttcgc ggcccagctg caccgcatct cgtccacgct gaccatacgc
      121 gacctgcacg aggaaatcga acagcgcggc catcagcacc gcgctgaggt gcagctggtg
      181 cgcgccgagc ccgcgcccgc tgagctggcc acccggctgg gtctggcgga gggcgacgaa
      241 gtcttccaca gcctgatcgt gcaccgcgaa aacggcgttg cgatccaatg tgaggaccgc
      301 tacgtcaacc cggcctgcgc gccgggctac cttagcgtcg atttcacccg caccacgccg
      361 acccaatacc tgctgcaggt cgcccccttg ggcgaagccg aatacagcat cgaggccagc
      421 ctgccgaccg cgcgcgaggc caagctgctg ggcatagaac gcggcgagcc ctgcctggtg
      481 gtggtcaggc gcacaacgag ccgcggccgg ccggtcacgc tggcgcgcct cgtgcatccg
      541 ggcacgcgct actgcctgga ggggcaattc aagccctgag cacatgagcg gcccccagtt
      601 gatccgcgtc gatgccatca agcccgagcc ctggcgtaat ggcggcggca gcacgcgcac
      661 cctgctcgcc tggccctcgg ccgagcactg ggacttgcgc atcagcgtgg cggagatcgc
      721 gatggacggc cccttctcgg cctaccccga catcgagcgc tggttcgcgg tgatcgaagg
      781 ggcgggcgtc gtgctgagca gcgagggaag caatctcgcg ctcgatcccg acagcccgcc
      841 gcaccgcttc gacggcgcga gcgcgccgca atgccggctg gtggatggcg caactcgcga
      901 cttgaacctg atgctcaggc aagacaggct cagcggcagc ctggagcgcg cgggttccgg
      961 tgtgcgctgg cagtccacgg cgcaactgca agggctgtac gccgacgacg ctggtcgcct
     1021 gagcgatgac gcaggccggc aatggcccct gcccgcacgg accctgtgct ggtggcaagc
     1081 cgcagccggc gagcctcggt cttggcgctt cgagtcggcc gcggcacatc cgctgcgagc
     1141 ctggtggtgg caggccgcac cgaccaagga ggcaagcaga tgagcctcac cctgtggcgc
     1201 catgcccatc tcgccacgct ggacggccgc gcgccctacg gctggctgga acatggcgcg
     1261 atgctcagcg aaggcgagac cttgcgctgg gtgggcgccg aggccgatct gccggcaacc
     1321 ctggcccgcc aggtgacggt cgagcatgac ctgggtggcg cctgcgtctc gccgggcctg
     1381 atcgactgcc acacccatct cgtctacggc ggccagcgcg ctcaggaatt cgagctgcgc
     1441 ctgcaaggcg cgagctacga ggagatcgca cgcgccggtg gcggcatacg ctccaccgtg
     1501 gccgccacgc gcgcggccag cgatgcgcaa ctgctggatt cggcaacgcg gcgcctgcaa
     1561 gccctgtgtg ccgagggcct caccacgatc gagatcaagt ccggttacgg cctgagcgcc
     1621 gagcacgagg cgcgctgcct gcgcatcgcg cgcaggctgg gcgagaagct gccgctgagc
     1681 gtgcgcacca cctacctcgc agcccacgcg ctaccgccgg aattcgaagg ccgcgcggac
     1741 gactacatcg acgccgtctg cgcctggctg ccgcagctgc atgcgcaggg tctggtcgat
     1801 gcggtcgatg ccttctgcga gcgcataggc ttcacgcccg cgcagacacg gcgcgtcttc
     1861 gacgcggcac ggcgcctagc gctgcccgtc aagctgcatg ccgagcagct gagcgaccag
     1921 ggcggcgcgg cgctggccgc cgagttcggc gcgctgtcct gcgatcacct ggaatacctc
     1981 ggcgagaccg gcgtgcaggc catggcccgt gccggcacgg tggccgtctt gctgcccggg
     2041 gcctactact ttttgcgcga gacgcagctg ccgcccgttg ccgcgctgcg ccggcatggc
     2101 gtgcccatcg cgatcgcgag cgaccacaac cccggcagtt cgccagcgct gtcgctgctg
     2161 ctgatgctca acatggcctg cacgctgttt cgcctgacgc ccgaagag
//
LOCUS       DASUCY010000548         3104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1153404, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000548 DASUCY010000000
VERSION     DASUCY010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153404"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VFV25_10800"
     CDS             <1..234
                     /locus_tag="VFV25_10800"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEY1393858.1"
                     /translation="AFETLGAKLVGWRTDNFNFASQRAIERLGAKRDGVLRHHALRRD
                     GTVRDTVMYSLTAGEWPEVRAHLRWQLARPREV"
     gene            311..2341
                     /locus_tag="VFV25_10805"
     CDS             311..2341
                     /locus_tag="VFV25_10805"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEY1393859.1"
                     /translation="MANKERGFSVEELWQIERLSGASLAPDGAQTVVSATRYSMEDNK
                     SSASLWLLSTLGGAPRRLTSCGEKDGQAQWSPTGDAIAFVAKREQEGKKDETPQLYLI
                     APDGGEARRISDYAPGIEAFKWFPDGKRIAFVSWVWPELKGAKAQTKMHKAFKERKES
                     GYATSEAQYRYWDHFVPMGRVARLHVLDVASGAVADLFEGTRHELTRADPGANDFDVS
                     PDGKRIVFGYDPEPEQRVDHCRALVELSIKGIKPSGKARELVRDKAWDCHAPRYSPDG
                     KRIAFLASHQGKKHTMPSHLAVVEGGRWQVLSADWDHSVHAPLRWAADGSCVYFTAED
                     RARHHLWRFALAERRAEIAVRGGWLQGFDVVPGVDGEVIVSVADAMDHPARVHVHASG
                     AEPRRIERFNDALLEKFSFGKHEEIIVKGARGDAVQTWLIHPPGFDNKAGAKKKYPLL
                     HVIHGGPHAAAGDTWHYRWNNQVFAAQGYVVACVNYHGSSGFGYDFLDSITHHWGEYE
                     LQDVEAATDALVKRPYVDAKRVFATGGSYGGYMVAWMNGHARPGRYRAYICHAGCFDW
                     TAMFADDAYFWHSKELGGWYWDAPAQVNKQSPHQYASGMKTPTLVIHGQLDYRVPDAQ
                     GLAYYNTLKARGVDARLLWFPDENHWILKPRNSRLWYGEFFDWLRRHDLRDA"
     gene            complement(2367..2930)
                     /locus_tag="VFV25_10810"
     CDS             complement(2367..2930)
                     /locus_tag="VFV25_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013588330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HEY1393860.1"
                     /translation="MNKRSFLATCSLAALLCACTTTSGVGGDPAAKRRSIDADVDSAL
                     SKLYAQVSGSKELAAKARGVLVFPSVVSAGFVLGGSYGEGALRAGGHTEGYYSTVAGS
                     VGLLAGADSKAVYLLFMTQEALDKFRASGGWTAGADASVTMVTVGANAGVDTTTARQP
                     VVGYVLSNAGLMANLSLDGTKITKLQL"
BASE COUNT          561 a         1065 c         1020 g          458 t
ORIGIN      
        1 gccttcgaga cgctgggcgc caagctcgtg ggctggcgca ccgacaactt caatttcgcg
       61 agccagcgcg cgatcgagcg cctgggcgcg aagcgcgacg gtgtgctgcg ccatcacgcg
      121 ctgcgccgcg acggcacggt gcgcgacacg gtgatgtaca gcctcacggc cggcgaatgg
      181 cctgaggtgc gtgcccacct gcgctggcag ctcgcgcggc cccgggaggt ctaaccgcaa
      241 gtcctacaag aaattcacgg tccaccttga tatttagcca atatcaacaa caaataaggt
      301 aacacgcaag atggccaaca aggaacgcgg cttcagcgtc gaggagctgt ggcagatcga
      361 acgcctgtcc ggcgcgagtc tcgctcccga tggtgcccag accgtggtct ctgccacgcg
      421 ctacagcatg gaggacaaca agagcagcgc ctcgctgtgg ctgctgtcca cgctgggcgg
      481 cgcgccgcga cgcctcacga gctgcggcga gaaggacggc caggcgcaat ggtcgcccac
      541 cggcgatgcg atcgccttcg tcgccaagcg cgagcaggaa ggcaagaagg acgagacgcc
      601 gcagctctac ctcatcgccc ccgacggcgg cgaggcgcgg cgcatcagcg actacgcgcc
      661 cggcatcgag gccttcaagt ggttcccgga cggcaagcgc atcgccttcg tgtcctgggt
      721 ctggcccgag ctcaaaggcg ccaaggctca gacgaagatg cacaaggcgt tcaaggagcg
      781 caaggaaagc ggctacgcga ccagcgaggc gcagtaccgc tactgggacc atttcgtgcc
      841 gatgggccgc gtcgcgcggc tgcatgtact cgatgtggca agcggcgccg ttgccgatct
      901 cttcgaaggc acgcgccacg agctcacgcg cgccgacccg ggcgccaacg acttcgacgt
      961 ctcgcccgac ggtaagcgca tcgttttcgg ctacgacccc gagcccgagc agcgcgttga
     1021 ccattgccgc gcgctggtcg agctgagcat caagggcatc aagcccagcg gcaaggcgcg
     1081 cgagctggtg cgcgacaagg cctgggactg ccacgccccg cgctacagcc ccgacggcaa
     1141 gcgcatcgcc ttcctcgcca gccaccaggg caagaagcac accatgcctt cgcatctggc
     1201 tgtcgtggaa ggcggccgct ggcaggtgct gagcgccgac tgggaccatt cggtccacgc
     1261 gcctctgcgc tgggcggccg acggttcatg cgtctacttc acggccgagg atcgcgcgcg
     1321 ccaccacttg tggcgctttg cgctcgccga gcgccgcgcc gagatcgccg tgcgcggcgg
     1381 ctggctgcag ggcttcgatg tcgtgcccgg cgtggacggc gaggtcatcg tcagtgtggc
     1441 cgatgcgatg gaccacccgg cgcgcgtgca tgtgcacgcc tccggcgccg agccgcggcg
     1501 catcgagcgc ttcaacgatg cgctgctgga gaagttcagc ttcggcaagc acgaggaaat
     1561 catcgtcaag ggcgcgcgcg gcgacgcggt gcagacctgg ctcattcatc cgccgggctt
     1621 cgacaacaag gccggcgcca agaagaaata cccgctgctg cacgtgatcc acggcggccc
     1681 gcacgcggcc gcgggcgaca cctggcatta ccgctggaac aaccaggtct tcgcggccca
     1741 gggctatgtg gtggcctgcg tcaactacca cggctccagc ggcttcggct acgacttcct
     1801 cgacagcatc acccaccatt ggggcgagta cgagctgcag gacgtcgagg ccgcgaccga
     1861 cgcgctggtc aagcgccctt acgtcgatgc caagcgcgtc ttcgccaccg gcggcagcta
     1921 tggcggctac atggtcgcgt ggatgaacgg ccatgcgcgg cccggccgct accgcgctta
     1981 catctgccac gccggctgct tcgactggac cgcgatgttc gccgacgacg cctacttctg
     2041 gcactccaag gagctaggcg gctggtattg ggacgcaccg gcccaggtca acaagcagag
     2101 cccgcatcag tacgcgagcg gcatgaagac gccgacgctg gtgatccacg gccagctcga
     2161 ctaccgcgtg cccgacgccc agggcctggc ctactacaac acgctcaagg cccgaggcgt
     2221 cgacgcgcgg ctgctgtggt tccccgacga gaaccactgg atcctgaagc cgcgaaacag
     2281 ccggctctgg tacggcgagt tcttcgactg gctcaggcgc cacgacctgc gcgacgcctg
     2341 aggccagatc gcgcgcaggc gcgcgctcag agctgcagct tggtgatctt ggtgccgtcc
     2401 aggctcaggt tcgccatcag gccggcgttg gacaacacat agcccaccac cggctgccgg
     2461 gccgtggtgg tgtccacgcc ggcgttggcg ccgaccgtta ccatcgtgac cgaggcgtcg
     2521 gcgccggcgg tccagccgcc gctggcgcgg aatttgtcga gcgcttcctg cgtcatgaac
     2581 agcaggtaga ccgccttcga gtcggcgcct gccagcaggc ccaccgagcc ggccaccgtg
     2641 ctgtagtagc cctcggtatg gccgccggcg cgcagcgccc cttcaccata ggagccgccc
     2701 aggacgaaac cggccgacac cacggagggg aagaccagca cgcccctggc cttggccgcc
     2761 agctccttgg agccggacac ctgcgcatag agcttgctga gagcactgtc cacatcggca
     2821 tctatgctgc gccgcttggc cgccgggtcg ccgccgacac cgctggtggt cgtgcaggcg
     2881 cacagcagcg cggcaagaga gcaagtcgca agaaaacttc gcttgttcat gttcgcatct
     2941 cctcaagcag agcggcgccc gagcaagggc cttgcgggtg caccgcttaa ttccaggcta
     3001 agccaagcag catggcaaat ccagccggaa ccgttacaaa tgcgactcga tgcgacagcg
     3061 cattgacatg ctgattgtga ccggctggca tcgaggacgg agcg
//
LOCUS       DASUCY010000549         3744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_558702, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000549 DASUCY010000000
VERSION     DASUCY010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558702"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1140)
                     /gene="rho"
                     /locus_tag="VFV25_10815"
     CDS             complement(<1..1140)
                     /gene="rho"
                     /locus_tag="VFV25_10815"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEY1393861.1"
                     /translation="MHLSELKALHISALTKMAEELEIENTGRLRKQELMFAIMKKRAK
                     QGEQIFGDGVLEVLPDGFGFLRSPDASYLASTDDIYLSPSQIRRFNLHTGDAIEGEVR
                     VPKDGERYFALVKVDRVNGLASEESKHKIMFENLTPLFPKEQFKLERDIKGEENITGR
                     IIDLIAPIGKGQRALLVAQPKTGKTVMMQHLAHAIVANHPEVYLIVLLVDERPEEVTE
                     MLRTVRGEVISSTFDEPAARHVQVAEMVIERAKRLVELKKDVVILLDSITRLARAYNN
                     VLPSSGKVLTGGVDANALQRPKRFFGAARNVEEGGSLTIIGTALVDTGSRMDEVIYEE
                     FKGTGNCEIHLDRRMAEKRVYPSILLNKSGTRREELLLKPEILQKT"
     gene            complement(1367..1696)
                     /gene="trxA"
                     /locus_tag="VFV25_10820"
     CDS             complement(1367..1696)
                     /gene="trxA"
                     /locus_tag="VFV25_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEY1393862.1"
                     /translation="MSSELIKHISDASFDGDVLKSDKPVLVDYWAEWCGPCKMIAPIL
                     DEVAKDYGGKLQITKMNVDDNREVPARYGIRGIPTLMLFKGGQHTATKVGALSKAQLT
                     AFLDQHL"
     gene            1853..>3744
                     /locus_tag="VFV25_10825"
     CDS             1853..>3744
                     /locus_tag="VFV25_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393863.1"
                     /translation="MFEQQRIAPALVDAAWWPAFARRIAAWLDAHELRAGDVVVLLPF
                     AQHLPLARQAWAGLGRPWMPRFETTQTLARSLAPAPQAGAGELSFDTATDRLTAMGWL
                     RKQGWAPRDPRGLEQAAARVVELAHGLARSAADFAPMDRAAYYEQARALLSPGSGPGA
                     QEKALARLALEWAALSSAASSDALFEHRPAAWIALLAGGADELVHRLLERAAAAGQPC
                     LQLDADPPPDTPFAGLPAPGLRVCNDFEHEAQCTAAEVLARLSEIKEEAAQVALIALD
                     RVLVRRVRALLECRGVSLADETGWKLSTTRAAAAVMSLLRAARSRASGDELLDWLKSG
                     DLAWPGLDAAVPLEHRIDALERAMRRHGWVRAWDASIGSTVAEADPGAAELWQQAQQA
                     LAPLRALPRSTSLIEALRYLRDALHACGAWEALSADAAGRLVIEALNLDVEAVPCSAW
                     QTSAAQARVDLEGLIAWADAALEQVSFRPPAEEAHAQVVITPLARALLRPFAAIVLPG
                     CDESHLGAAPTPPPLIGDALATQLGLPGLAAQRARELAAFALLLRVPVVSLLRRESDS
                     ESALAPSPLLERLLLSLRREQREAAALPERRISRRLALQPVRRPLPRLGRQPELLPRQ
                     LSATAC"
BASE COUNT          508 a         1254 c         1306 g          676 t
ORIGIN      
        1 ggtcttttgc aggatttccg gcttgagcag cagttcctcg cggcgcgtac cgctcttgtt
       61 gagcaggatg gacgggtaga cgcgcttctc ggccatgcgg cggtcgaggt ggatctcgca
      121 gttgccggtg cccttgaact cctcgtagat cacctcgtcc atccggctgc ccgtgtcgac
      181 cagcgcggtg ccgatgatgg tcagcgaccc gccttcctcg acgttgcgcg ccgcgccgaa
      241 gaagcgcttg gggcgctgca gcgcattggc gtccacgccg ccggtcagca ccttgcccga
      301 cgacggcagg acgttgttgt aggcgcgcgc cagccgcgtg atggagtcga gcaggattac
      361 cacgtccttc ttcagctcga ccaggcgctt ggcgcgctcg atcaccatct cggcgacctg
      421 cacgtgccgc gccgccggct cgtcgaaggt cgagctgatg acttcgccgc gcacggtgcg
      481 cagcatctcg gtcacttcct cggggcgctc gtcgacgagc agcacgatca ggtagacctc
      541 gggatggttg gcgacgatgg cgtgggccag gtgctgcatc atcaccgtct tgccggtctt
      601 gggctgggct accagcaagg cacgctggcc tttgccgatg ggggcgatca ggtcgatgat
      661 gcgcccggtg atgttctctt cgcccttgat gtcgcgctcg agcttgaact gctccttggg
      721 gaagagcggc gtcaagtttt cgaacatgat cttgtgcttg ctctcctcgg acgccaggcc
      781 attgacgcgg tcgaccttga ccagcgcgaa atagcgctca ccgtccttgg gcacgcgcac
      841 ctcgccttcg atggcatcac cggtgtgcag gttgaagcgg cggatctggc tgggcgacag
      901 atagatgtcg tcggtgcttg ccagatagct cgcatcgggc gagcgcagga agccgaagcc
      961 gtcgggcagc acttcgagca cgccgtcgcc gaagatctgc tcgccctgct tggcgcgctt
     1021 cttcatgatc gcgaacatca gctcctgctt gcgcaggcgg ccggtgttct cgatctccag
     1081 ctcttcggcc atcttggtca gcgccgagat gtgcagtgct ttgagttctg acaaatgcat
     1141 gagggattac ccttcaaggg gaaccgtcgt cttgccagga ttgacggctg gagctgtgcc
     1201 ggccctgcag cgtgcaccgg taggggcgca cggcctcgcg ttgtgagttg tctgtatgga
     1261 acgcgggaga gggggccgac tcggcgcgct tggaacctgc gccgtggcta tcggggggcg
     1321 gccgggcggt ggcttgtggc tcacgcgccc ggtgcgtctg gcgggattat aggtgctggt
     1381 cgaggaaggc cgtcaactgg gccttggaaa gtgcgcccac tttcgtggcc gtgtgctggc
     1441 cgcccttgaa gagcatcagc gtcgggatgc cgcggatgcc gtagcgggcc ggcacttcgc
     1501 ggttgtcgtc gacgttcatc ttggtgatct gcagcttgcc gccgtagtcc ttggcgacct
     1561 cgtcgaggat cggcgcgatc atcttgcagg gaccgcacca ctcggcccag tagtcgacca
     1621 gcacaggctt gtcggacttc agcacgtcgc cgtcgaaaga ggcgtcggag atatgtttga
     1681 tcagttcgct gctcatcgtt gcgtcctcgg ggtccacggg ggcaaggcac aatgattttg
     1741 gcacaaagcc cgaaattgca agggtgcgtg ccggccctgt ttgatgccag tgcggctatt
     1801 gccgccatag tcgacgcggt cccccggcgc gatatcttcc tcccccgaat tcatgttcga
     1861 gcaacagcgc atcgctcccg cactcgtcga cgctgcatgg tggcccgcct tcgccaggcg
     1921 catcgccgct tggttggatg cgcatgagct gcgcgccggc gacgtcgtcg tgctgctgcc
     1981 ctttgcccag catctgccgc ttgcacgtca ggcctgggcc ggactggggc ggccctggat
     2041 gccgcgtttc gaaaccactc agacgctggc tcgctccctg gcgcccgcgc cccaggcagg
     2101 cgcgggagag ctgagcttcg acaccgccac cgacaggctc acggccatgg gctggctgcg
     2161 caagcaaggc tgggcgccgc gcgatccgcg cggcctggag caggccgcag cccgggtggt
     2221 cgagctggcc catggcctgg cgcgcagcgc tgccgacttt gccccgatgg atcgcgccgc
     2281 ctattacgag caggcgcgcg ccttgctgtc gccaggcagc ggccccggcg cacaggaaaa
     2341 agcgctggcc cgcctggcgc tggaatgggc cgcgctatct tcggcggcga gcagcgatgc
     2401 cttgttcgag cataggcccg cggcctggat cgccctgctg gcgggcggcg ccgacgagct
     2461 ggtgcatcgc ctgctggagc gcgccgctgc agcgggccag ccctgcctgc agctggatgc
     2521 ggacccgccg cctgacacgc ctttcgctgg gctgccggcg ccgggcttga gagtttgcaa
     2581 cgatttcgag cacgaggccc aatgcacggc ggccgaagtg ctggcccgcc tgtccgagat
     2641 caaggaggaa gccgcccagg ttgccctcat cgcgctggac cgcgtgctgg tgcgccgcgt
     2701 gcgggcactg ctggagtgcc gcggcgtcag cctggcggac gagaccggct ggaagctgtc
     2761 gaccacgcgc gctgccgccg ccgtgatgag cctgctgcgc gcggcccgct cgcgcgccag
     2821 cggcgacgaa ctgctcgact ggctcaagtc cggcgacttg gcctggccgg gtctcgacgc
     2881 tgcggttccg ctcgagcaca ggatcgatgc cctggagcga gcgatgcgac gccacggctg
     2941 ggtgcgggcc tgggatgcct ctatcggcag cacggtcgcc gaagcggacc cgggtgcggc
     3001 cgagctttgg caacaggcgc agcaggcgct ggcgccgttg cgcgcgctgc cgcgttcgac
     3061 ttcgctgatc gaggccctgc gctatctgcg cgatgcgctg cacgcatgcg gcgcctggga
     3121 ggcgctgtct gcagacgcgg ccggtcgcct ggtgatcgag gcactgaacc tggacgtgga
     3181 ggccgttccc tgctcggcct ggcaaaccag cgcggcccag gcccgtgtcg acctggaggg
     3241 cctgatcgcc tgggccgatg cggcgctgga gcaggtcagc tttcggccac ccgccgaaga
     3301 agcgcacgcg caggtggtga tcacgccgct ggcgcgcgcc ctgctgcggc ctttcgcggc
     3361 catcgtgctg ccgggctgcg acgagtcgca ccttggcgcg gcgccaacac cgccgccctt
     3421 gatcggcgat gcactggcca cccagctggg cctgcccggc ttggccgccc agcgcgcgcg
     3481 cgaattggct gccttcgcct tgctgctgcg cgtgcctgtg gtcagcctgc tgcgacgcga
     3541 atcggacagc gaatcggcgc ttgcccctag ccccttgctg gagcgcctgc tcctgagcct
     3601 gcgccgcgaa cagcgcgaag ccgccgcgct gcccgagcgg cgcatctcgc ggcgcttggc
     3661 cttgcagccg gtgcgccggc ctttgccgcg cctggggcgc cagcccgagc tgctgccgcg
     3721 ccagctcagc gccaccgcct gcga
//
LOCUS       DASUCY010000550         2260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1586684, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000550 DASUCY010000000
VERSION     DASUCY010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1586684"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..100
                     /locus_tag="VFV25_10830"
     CDS             <1..100
                     /locus_tag="VFV25_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEY1393864.1"
                     /translation="SYLDSRARLGFPMAPAAWGQEVMAQLVKKKAA"
     gene            102..>2260
                     /gene="glyS"
                     /locus_tag="VFV25_10835"
     CDS             102..>2260
                     /gene="glyS"
                     /locus_tag="VFV25_10835"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEY1393865.1"
                     /translation="MTTKKNLLVELFVEELPPKALKQLGEAFAVSIFNLLEKAQLVLR
                     AEVADVKTFASPRRLAVFIPNVQLRAIDRSESQKLMPVSVGLDAQGQATPALLKKLAA
                     LGAGPEAVGALKRQQDGKAEALYFDSHVAGATLAEGLQKALDEALAKLPIPKLMSYQL
                     EDGWTSVSFVRPAHGLVALHGADVVTIAALGLEAGRETHGHRFEAAVDPIALRDADSY
                     AAQLEEQGAVIASFEARKAEIARQLAAAADRVGKDDGSHGALRPIDDEALLDEVTALV
                     ERPNVLVCEFERDFLAVPQECLILTMKANQKYFPLLDAQGKLTNRFLIVSNIRPADAS
                     AVIGGNERVVRPRLADAKFFFDQDRKKTLAARVPGLDKVVYHGKLGSQGERALRVQAI
                     AHAIVNQLRMATVPYTAEAKDEFDVLDSKVKEAALLAKADLLTDMVGEFPELQGIMGG
                     YYARHEGLRDGVAIAIEDHYKPRFAGDALPRNHTGVVLALADKLETLVGLFGIGQLPT
                     GDKDPFALRRHALGVIRLLTERNLPLSLPALIDAAVPVFGERIADSSAALLDFFFDRL
                     AVSLREQGYSAQEVDAVLALRPERLGELPRRLAAVRAFAGLPESASLAAANKRVGNIL
                     KKAEGAVDAKVNAALLKEPAEIALNDALKSVATKAENAWVIGNYTENLQVLAALKAPV
                     DSFFDDVMVNAEDPALRANRLGLLATLHAAMNRVADLS"
BASE COUNT          392 a          726 c          782 g          360 t
ORIGIN      
        1 gagctatctg gacagccgcg cccgcctggg cttcccgatg gcgccggccg cctggggcca
       61 ggaagtgatg gcccaacttg taaagaaaaa ggcggcttga gatgacgacc aagaagaacc
      121 tgctggtcga gctcttcgtc gaggagctgc cgcccaaggc gctgaagcag ctgggcgagg
      181 cttttgctgt ttccatcttc aacttgcttg agaaagcgca gttggtgctt cgggcggaag
      241 tcgcagacgt caagaccttc gcaagtcccc gtcgtttggc agtgtttatt ccgaacgtgc
      301 agttgagagc aatagatcgc tccgagtctc agaagctgat gcccgtaagc gttggcctcg
      361 acgcccaggg ccaagccacg ccggcgctgc tgaagaagct ggccgcgctg ggcgcaggcc
      421 ccgaggcggt gggcgcactg aagcgccagc aggatggcaa ggccgaggcg ctgtacttcg
      481 acagccatgt tgcgggtgct acgctggccg agggcctgca gaaggcgctg gacgaagcgc
      541 tcgccaagct gcccatcccc aagctgatga gctaccaact cgaagacggc tggaccagcg
      601 tgagcttcgt gcgccccgcg catggcctgg tcgcgctgca cggcgccgac gtggtgacga
      661 tcgccgcgct gggcctggag gccgggcgcg agacgcatgg ccaccgcttc gaggctgctg
      721 tcgatcccat cgcgctgcgc gatgccgaca gctatgcggc gcagctagaa gagcagggcg
      781 cagtgatcgc gagcttcgag gcgcgcaagg ccgagattgc gcgccagttg gccgcggcgg
      841 cggaccgcgt gggcaaggat gacgggtcgc acggcgcgct ccggcccatc gacgacgagg
      901 ccctgctcga cgaggtgacg gcgctggtcg agcgacccaa tgtgctggtc tgcgagttcg
      961 agcgggactt cctcgcggtg ccgcaggaat gcctcatcct cacgatgaag gccaaccaga
     1021 aatacttccc gctgctcgac gcgcagggca agctcaccaa ccgcttcctc atcgtcagca
     1081 acatccggcc ggccgatgcg agcgctgtga tcggcggcaa cgagcgagtg gtgcgtccgc
     1141 gcctggcgga cgcgaaattc ttcttcgacc aggaccgcaa gaagacgctg gccgcgcgcg
     1201 tgccgggtct ggacaaggtg gtctatcacg gcaagctcgg cagccagggc gagcgggcgt
     1261 tgcgtgtgca ggccatcgcg cacgccatcg tcaaccagct gcggatggcg actgtgccct
     1321 acaccgcgga ggccaaggac gagttcgatg tgctcgacag caaggtcaag gaggccgcgc
     1381 tgctggccaa ggccgacctg ctgaccgaca tggtgggcga gttccccgag ctgcagggca
     1441 tcatgggcgg ctactacgcg cgccacgagg gcctgcgcga cggcgtggcc atcgccatcg
     1501 aggaccacta caagccgcgc ttcgccggcg acgcgctgcc gcgcaaccac accggcgtcg
     1561 tgctggcatt ggccgacaaa ctcgaaacgc tggtgggtct gttcggcatc ggccagctgc
     1621 ccaccggcga caaggatccg tttgcgctgc gccgccatgc gctcggcgtg atccgtctgc
     1681 tgaccgagcg caacctgccg ctgtcgctgc cggcgctcat agacgcggcc gtgcccgtgt
     1741 tcggcgagcg catcgccgat tcgagcgctg cgctgctgga cttcttcttc gaccgcttgg
     1801 cggtgtcgct gcgcgagcag ggttacagcg cgcaggaagt ggatgccgtg ctggcgctgc
     1861 gccccgagcg cctgggcgag ctgccgcgcc ggctggctgc ggtgcgcgcc ttcgcaggcc
     1921 tgcccgagtc ggcgtcgctg gcggccgcca acaagcgcgt gggcaacatc ctgaagaagg
     1981 ccgaaggcgc ggtggatgcc aaggtcaatg cggccttgct caaggagccg gccgagatcg
     2041 ccttgaatga cgctctgaaa tctgtagcga ctaaggccga aaatgcttgg gttataggca
     2101 attacaccga gaatctgcaa gtcctggcgg cgctgaaggc tccggtggac agcttcttcg
     2161 acgatgtgat ggtcaacgcc gaagaccccg ccctgcgcgc caaccgcctg ggcctgctgg
     2221 cgaccctgca tgccgcgatg aaccgcgtgg ccgacctgtc
//
LOCUS       DASUCY010000551         4434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_880093, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000551 DASUCY010000000
VERSION     DASUCY010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_880093"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VFV25_10840"
     CDS             <1..201
                     /locus_tag="VFV25_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HEY1393866.1"
                     /translation="LPWLGVVWWGLAAGRWVLAQRPGWIDGSLAAPARPLAMLGRWSL
                     SFYMLHQPILIGALMLWRYLVR"
     gene            225..1652
                     /gene="mdtD"
                     /locus_tag="VFV25_10845"
     CDS             225..1652
                     /gene="mdtD"
                     /locus_tag="VFV25_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HEY1393867.1"
                     /translation="MVTTTQLDAGTKRVLPWLVAIGFFMQTLDSTILNTALPRMALDL
                     HESPLRMQSVVTAYMLTVALLIPVSGWLADRLGTRRVFLSAIVLFSLGSLLCALSGSL
                     TLLIASRVLQGVGGALLLPVGRLAILRAFPREQLLPALAFVTVPGLVGPLVGPTLGGW
                     LVEVASWHWVFLINLPVGVVGLLATARYMPNLRSPAPPRLDLTGFALFGAGMVMMSMA
                     LQGLGERALGLGASMVLLFAGLASLSGYWLHAARAEAPLFSLQLFHIPSFSVGLLGNL
                     FARLGSGAMPYLTPLFLQVGLGYAPTMAGLSMIPTALGAMLSKTFATPLIRNLGYRRT
                     LVINTLLLGCMIASFALVRQGIQHWLLLVQLGLFGIVNSLQFTAMNTLTLRDLDERSA
                     SGGNSLLSVVMQLSMSLGVAAAAALLAAFGGREQLGGSEAVVDTFHATYICVGILSAL
                     AAFIFSQLERAKPAEEARASTLDTA"
     gene            complement(1743..2549)
                     /locus_tag="VFV25_10850"
     CDS             complement(1743..2549)
                     /locus_tag="VFV25_10850"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEY1393868.1"
                     /translation="MKKLLLPAVVGTVVLSTVLSFPLIAAAQNLAMVNGKPVPKSRVT
                     LLMSQVEKQGQAPTPQLEKQVKDEVVMREIFMQEAQKRGLQSSDTYKEQMELARQSIL
                     IRELFSDYAKKNPVTDDEIQAEYAKFKAKAGDKEYHARHILVDKEEDAKALIAKIKAG
                     AKFEDLAKANSKDPGSAQNGGDLEWASPSSYVPEFSQALTQLQKGQMTDAPVKTSFGW
                     HIIKLEDVRAATVPSLDEMKPQIQQFLGQQKLGAFRDELKAKAKTDYKFD"
     gene            complement(2665..2922)
                     /locus_tag="VFV25_10855"
     CDS             complement(2665..2922)
                     /locus_tag="VFV25_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HEY1393869.1"
                     /translation="MAVTAEQIDAVLRQAFNPQSLEVRDDSHLHAGHAGAREGRHFSV
                     HIVSASFTGLNRLARHRLVYDVLFDLVPQGIHALAIKADAP"
     gene            complement(2928..3584)
                     /locus_tag="VFV25_10860"
     CDS             complement(2928..3584)
                     /locus_tag="VFV25_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEY1393870.1"
                     /translation="MKLLFDFLPILLFFATFKLTEGHSDAAAAFATEHFGFLVSGGIV
                     GHTEAPVLLATLVVIAATFAQVIYLKARRRKVDTMLWVSLGLVTVLGGATVWFHNETF
                     IKWKPSVLYWVMGLALWLGQALFHKNLLQRLMGNQLQLPAPVWQRLSVMWIAFFGLMG
                     LLNLYVAYSYSTSTWASFKLFGTMGLMLLFTLAQGLYLARYLEADASAEATDEPAADA
                     "
     gene            complement(3651..4058)
                     /gene="msrB"
                     /locus_tag="VFV25_10865"
     CDS             complement(3651..4058)
                     /gene="msrB"
                     /locus_tag="VFV25_10865"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEY1393871.1"
                     /translation="MSADYKVKKTEAEWKAQLDKSQFEVTRHSATERPFTGEYWDHWK
                     DGVYHCVCCGTPLFSSATKFDAGCGWPSYFQPLQSEVVERLEDRSHGMVRVEVRCNNC
                     GAHLGHVFEDGPQPTGERFCINSAALDFKDRAE"
     gene            complement(4055..>4434)
                     /locus_tag="VFV25_10870"
     CDS             complement(4055..>4434)
                     /locus_tag="VFV25_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein adenylyltransferase SelO family protein"
                     /protein_id="HEY1393872.1"
                     /translation="VDFSIAFRRLSGFSTAEGAVNESVRDLFLDRAGFDAWAATYARR
                     LRAEASIDAERALRLKRVNPKFVLRNHLAELAIRQAQQGDFDEIHRLLKVLQHPYDEQ
                     PEHSAYAGFPPDWAQTIAVSCSS"
BASE COUNT          686 a         1411 c         1481 g          856 t
ORIGIN      
        1 ctgccctggc tgggcgtggt gtggtggggg ctggcggcgg gccgctgggt gctggcgcag
       61 cgtcccggct ggatcgacgg ctcgctcgcc gccccggcgc gcccgctggc catgttgggg
      121 cgctggagcc tgagcttcta catgctgcac cagcccatcc tgatcggcgc tctgatgctg
      181 tggcgttact tggtccgatg actcgtcccg acaaagcctg actcatggtg acaacaacgc
      241 aactcgatgc cggcaccaaa cgcgtgctgc cctggctggt tgccatcggc ttcttcatgc
      301 agacgctgga ctcgaccatc ctcaacaccg ccttgccgcg catggcgctg gacctgcacg
      361 agagcccgct gcgcatgcag tcggtggtga cggcctacat gctgacggtg gcgctgctga
      421 tcccggtctc gggctggctg gccgaccgcc tgggcacgcg ccgcgtgttc ttgagcgcca
      481 tcgtgctgtt cagcctgggc tcactgctgt gcgccctgtc cggctcgctg acactgctga
      541 ttgcctcgcg ggtgttgcag ggcgtcggcg gagccttgct gctgccggtg ggccggctgg
      601 cgatcctgcg cgcctttccc cgcgagcagc tgctgccggc cctggctttc gtcaccgtgc
      661 ccgggctggt cgggcccctg gtggggccga cgctgggtgg ctggctggtc gaagtggcga
      721 gctggcactg ggtcttcctg atcaacctgc cggtgggcgt cgtaggcctg ctcgccaccg
      781 cgcgctacat gcccaatctg cgcagccccg cgccgccgcg gctggatctc acgggcttcg
      841 cgctcttcgg cgccggcatg gtgatgatgt cgatggccct gcagggcctg ggcgagcgcg
      901 cgctgggact gggcgcctcg atggtgctgc tgttcgccgg cctggccagc ctgtccggct
      961 attggctgca tgccgcgcgc gccgaggcgc cgctgttcag cctccagctc tttcacatcc
     1021 ccagcttctc ggtcggcttg ctcggcaatc tgttcgcccg cctgggcagc ggtgccatgc
     1081 cgtatctcac gccgctcttc ctgcaggtgg gcctgggcta tgcgcccacg atggcgggct
     1141 tgagcatgat cccgacggcc ttgggcgcga tgctgtccaa gaccttcgcg acgccgctga
     1201 tccggaacct gggctatcgg cgcaccctgg tcatcaacac gctgctgctg ggctgcatga
     1261 tcgccagctt cgcgctggtg cgccagggca tccagcattg gctgctgctc gtgcagctgg
     1321 gcctcttcgg catcgtcaac tcgctgcagt tcacggcgat gaacacgctc acgctgcgcg
     1381 atctggacga acgcagcgcg agcggcggca acagcctgct gtcggtggtg atgcagctgt
     1441 cgatgagcct gggcgtcgcg gccgccgccg ccctgctggc cgccttcggc gggcgcgagc
     1501 agctgggcgg ttccgaggca gtagtggaca ccttccacgc aacctacatc tgcgtaggca
     1561 tcctgtcggc cctggcggcc ttcatcttct cgcagctcga acgcgcaaag cctgccgagg
     1621 aggcgcgcgc ttccaccttg gacacggctt gatcgtcatc ccgatcacgg ccgctgcaag
     1681 aaccggcaca cagcaatgaa aaaggcgccc cgtggggcgc ctttttcttc cgtgcaggcc
     1741 gatcagtcga acttgtagtc ggtcttggcc ttggccttca gctcgtcgcg gaaggcgccg
     1801 agcttctgct gacccaggaa ctgctggatc tggggcttca tttcgtcgag cgacggaacg
     1861 gtggcggcgc gcacgtcctc gagcttgatg atgtgccagc cgaaggaggt cttgaccggg
     1921 gcatcggtca tctggccctt ctgcagctgg gtcagtgcct gcgagaactc gggcacatag
     1981 ctgctggggg aagcccactc gagatcgccg ccgttctgcg cggagcccgg atccttgctg
     2041 ttggccttgg ccaggtcttc gaacttggcg ccggccttga tcttggcgat cagcgccttg
     2101 gcgtcctctt ccttgtcgac caggatgtgg cgggcgtggt attccttgtc gccggccttg
     2161 gccttgaact tggcgtactc ggcctggatc tcgtcatcgg tgaccgggtt cttcttggcg
     2221 tagtcggaga agagctcgcg gatcaggatg ctctggcgcg cgagctccat ctgctccttg
     2281 taggtatcgc tggactgcag gccgcgcttc tgcgcttcct gcatgaagat ctcgcgcatc
     2341 accacttcgt ccttgacctg cttttcgagc tgaggcgtgg gcgcctggcc ttgcttctcg
     2401 acttggctca tcagcaaggt gacgcgggac ttgggcaccg gcttgccgtt gaccatggcc
     2461 aagttctgcg ccgcggcgat caagggaaag ctcaacacgg tggacaggac cacggtgccg
     2521 acgaccgcgg ggagaagaag cttcttcatg tgaaggtcta acctttcgtg cgagaaaaat
     2581 aggggctgac gctctaggcc tcttgtgata ccagcgtacc ttgcgcggca cctgaagtcg
     2641 cgagacgcta aaacctagaa ccgctcaagg agcgtccgcc ttgatcgcca gggcatggat
     2701 gccttggggg accaagtcaa acagtacatc ataaacgagg cgatgacgcg ccaatctgtt
     2761 tagacctgta aagcttgcac tgacgatgtg cacgctgaag tgccgcccct cgcgggcccc
     2821 ggcatggcct gcgtgcagat ggctgtcgtc tcgcacctcg agcgactgcg ggttgaaagc
     2881 ctgccgcaag acagcatcga tttgttcagc ggtgacggcc atggcgctca ggcgtcggcg
     2941 gcgggttcgt cggtggcttc cgcggacgcg tccgcctcca gatagcgggc caaatacagc
     3001 ccctgggcca gcgtgaacag cagcatcagg cccatggtgc cgaagagctt gaagctggcc
     3061 caggtggaag tggaatagct gtaggccacg tagagattca gcaagcccat gaggccgaag
     3121 aaggcgatcc acatcacgct caggcgctgc caaacgggcg caggcagttg cagctggttg
     3181 cccatcagcc gctgcagcag gttcttgtgg aacagcgctt gccccagcca cagcgccaag
     3241 cccatgaccc agtagagcac gctgggcttc cacttgatga aggtctcgtt gtggaaccag
     3301 acggtggcgc cacccagcac cgtgacgagg ccgaggctca cccacagcat cgtgtccacc
     3361 ttgcgccggc gggccttgag atagatcacc tgggcgaagg tggcggctat caccaccagg
     3421 gtggcgagca gcaccggcgc ctcggtgtgg cccacgatgc cgcccgacac caggaagccg
     3481 aaatgctcgg tcgcgaaggc cgcagcggcg tcggaatgac cttcggtgag cttgaaggtc
     3541 gcgaagaaca gcaggatggg gaggaaatcg aaaaggagct tcatgcagga cggcaagggc
     3601 cgctgggtgg tgcatcagcc gggcactggt ctgggcctca ggctcaggtg ttattcggcg
     3661 cgatccttga agtctagcgc ggccgaattg atgcagaagc gctcacccgt cggttggggc
     3721 ccgtcctcga agacatggcc caggtgggcg ccgcagttgt tgcagcgcac ctcgacccgc
     3781 accatgccgt ggctgcggtc ttccaggcgc tcgacgactt cgctttgcag cggctggaag
     3841 tagctgggcc agccgcagcc ggcgtcgaac ttggtggccg agctgaatag tggcgtgccg
     3901 cagcacacgc agtggtagac gccgtccttc cagtggtccc agtactctcc tgtgaagggc
     3961 cgctcggtgg ccgagtggcg cgtcacctcg aactgcgact tgtcgagctg ggccttccac
     4021 tcggcctcgg tcttcttcac tttgtagtct gcgctcatga tgaacaggac accgctatgg
     4081 tttgcgccca atcgggcggg aacccggcat aggccgagtg ctcaggctgt tcgtcgtagg
     4141 gatgctgcaa aactttcagc aatcgatgga tttcgtcgaa atcgccctgc tgggcctggc
     4201 gtatcgccag ctcggccagg tggttgcgca gcacgaactt ggggttgacg cgcttcaggc
     4261 gcaaggcgcg ctcggcgtcg atgctggcct cggcgcgcag gcgccgggcg taagttgcgg
     4321 cccaggcgtc gaagcccgcg cggtcgagaa aaagatcgcg cacggactcg ttgaccgcgc
     4381 cctcggccgt gctgaagcct gacagccggc ggaaggcgat gctgaagtcc acgc
//
LOCUS       DASUCY010000552         3059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1570859, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000552 DASUCY010000000
VERSION     DASUCY010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1570859"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1984
                     /locus_tag="VFV25_10875"
     CDS             <1..1984
                     /locus_tag="VFV25_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013209139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY1393873.1"
                     /translation="SAVILVENIFRNFQKTPEERHALLDAYAGGLHPVSMPRAQGWTA
                     RLKLIFVSATQVDRAVLFSTAITVAAFIPLFTMEGVEGQIFGPMARTYGYALAGALLA
                     TFTVTPVLASFLLPHHVKETETWLVRKLREAYTPVLRWVLVHRNATVGLGLAFLLLAG
                     GLMLRTGTEFLPALEEGNLWIRATLPPTVSLEAGNRLVTRMREILKSHPEVITVVSQH
                     GRPDDGSDAAGFNNIELFCPLLPFDKFPAGSNKEKLLASLQAQFNDEFPGVELNFSQY
                     IQDNIEEGLSGIKGANSVKIVGPDLAVLERLADQVKHEMAQVPGVQDLGVFRVLGQPN
                     LDITVDRAQAARYGLNSGDVNAVVQAALGGTQASTIYEGDRQFGLVARLVPEARSSID
                     AVKRLQVSYTNASGGTSYVPLGDLARITLDTGASYIYHERNERFIPVKFSVRGRDLGS
                     TVAQAQARIAKNVALPQGYRLTWAGEFESLQLAKQRLMVILPIALIIILVLLYALFNS
                     LRDSLLTLAAIPFSIAGGVVALYISGLDLSVSAAIGFVSLFGVSVMNGILVITYFNHL
                     RAEGQAPMEAMFNAAEQRMRPMLMTALSACIGLLPAALSTGIGSQVQRPLATVVVGGM
                     LLGPVMLLLVAPALQMMFLGKTTRAQARAHGGVEEF"
     gene            1996..>3059
                     /locus_tag="VFV25_10880"
     CDS             1996..>3059
                     /locus_tag="VFV25_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEY1393874.1"
                     /translation="MRTANRREEEMSRHKPLVLAVLVLLGGCAVGPDYHRPALPESKA
                     YAPAPLPETSASAPLAGGEAQSFVAGRDIPFEWWKAYGSPALDALVERAFKANASLPA
                     AQAALRQAQELVYAQRGYFSPTVGLGYSFERQQLAGNQGGNSPGIQGNGSTIQTTQNP
                     GGPAPYNGPVIYNFHTAQLTVGYTPDVFGANRRQVESLEAQKDLAYYQLQAAYLTLAS
                     NVVAAAIQEASLRAQIAQTESIIAANEKSLQILQRQLATGYAMRLDVMAQETALAQAR
                     QTLPPLRKQLEQTRDLIRMLAGQPPDAEVEQRFELADLHLPQELPVSLPSKLVDHRPD
                     VRAAEEQLRSANAQVGAAVAA"
BASE COUNT          489 a         1071 c         1015 g          484 t
ORIGIN      
        1 ctcggcggtg atcctggtcg agaacatctt ccgcaacttc cagaagacgc ccgaggagcg
       61 ccatgcactg ctcgacgcct atgccggcgg cctgcatccg gtgtccatgc cgcgcgctca
      121 gggctggacg gcccggctca agctgatctt cgtcagcgcg acccaggtcg accgcgcggt
      181 gctgttctcc accgcgatca cggtggcggc cttcattccg ctgttcacga tggaaggcgt
      241 ggaaggccag atcttcgggc cgatggcgcg cacctacggc tatgcgctgg ccggggcgct
      301 gctggccacc ttcacggtga cgccggtgct ggcctccttc ctgttgccgc accacgtgaa
      361 ggagactgag acctggctgg tgcgcaagct gcgcgaggcc tacaccccag tgctgcgctg
      421 ggtgctggtg caccgcaacg ccaccgtggg gctgggcctg gccttcctgc tgctcgcagg
      481 aggtctgatg ctgcgcaccg gcaccgaatt cctgccggcg ctggaggagg gcaacctgtg
      541 gatacgcgcg acgttgccgc ccaccgtctc gctggaggca ggcaaccgtc tggtgacgcg
      601 catgcgcgag atcctgaaga gccaccccga ggtcatcacc gtggtctcgc agcatggccg
      661 gcccgacgac ggcagcgatg cggcgggctt caacaacatc gagcttttct gcccgctgct
      721 gcccttcgac aaattccccg ccggctcgaa caaggagaag ctgctggcca gcctgcaggc
      781 ccagttcaat gacgaattcc ccggcgtcga gctgaacttc tcgcaataca tccaggacaa
      841 catcgaagaa ggcctctcgg gaatcaaggg cgccaactcg gtgaagatcg tcggccctga
      901 tctcgcggtg ctggagaggc tggccgacca ggtcaagcac gagatggccc aagtgcccgg
      961 cgtgcaggac ctaggcgtgt tccgcgtgct gggccagccc aatctcgaca tcacggtcga
     1021 ccgcgcccag gcggcgcgct acgggctcaa ctccggcgac gtcaacgccg tcgtgcaggc
     1081 ggcgctgggc ggcacccagg ccagcacgat ctacgagggc gaccgccagt tcggcctggt
     1141 ggcgcggctg gtgcccgagg cgcgcagcag catcgatgcg gtcaagcggc tgcaggtgtc
     1201 ctacaccaac gccagcggcg gcacgtccta tgtgccgctc ggcgacctgg ccaggatcac
     1261 gctcgatacc ggcgcctcct acatctatca cgagcgcaac gagcgcttca tcccggtcaa
     1321 gttcagcgtg cgcgggcgcg acctgggcag caccgtggcg caggcccagg cgcgcatcgc
     1381 gaagaacgtc gcgctacccc aaggctaccg cctgacctgg gccggcgagt tcgagtcgct
     1441 gcagctcgcg aagcagcggc tgatggtgat cctgccgatc gcgctcatca tcatcctcgt
     1501 gctgctctat gcgctgttca actcgctgcg cgacagcctg ctcacgctgg cggccatccc
     1561 cttctcgatc gcgggcggcg tcgtcgcgct ctacatctct gggctggacc tgagcgtctc
     1621 cgcggcgatc ggcttcgtct cgctgtttgg cgtctcggtg atgaacggca tcctcgtcat
     1681 cacctacttc aatcacctgc gtgccgaagg gcaggcgccg atggaggcga tgttcaacgc
     1741 ggccgagcag cgcatgcggc cgatgctgat gacggcgctt tcggcctgca tcggtctgct
     1801 gccggcggcg ctgtccaccg gcatcggcag ccaggtgcag cggccgctgg ccaccgtggt
     1861 ggtgggcggc atgctgctcg ggccggtgat gctgctgctg gtggcgccgg ccctgcagat
     1921 gatgttcctc ggcaagacca cgcgggcgca ggcacgtgcg catggcgggg tcgaagagtt
     1981 ctaaggagac ggcctatgcg aaccgcgaat cgccgtgaag aagaaatgag ccggcacaag
     2041 ccgctcgtac tggcagtgct ggtcttgctg ggcggctgcg ccgtcggccc cgactaccac
     2101 cgccccgcgc tgcccgagag caaggcctat gcgcctgctc cgctgcccga aaccagcgcc
     2161 tccgcgccgc tggccggcgg cgaggcccag agcttcgtgg ccgggcgcga catccccttc
     2221 gagtggtgga aggcctatgg ctcgccggcc ctcgatgcgc tggtcgagcg cgccttcaag
     2281 gccaatgcca gcctgcccgc ggcgcaggcg gcgctgcgtc aggcgcagga gctggtctat
     2341 gcccagcgtg gctatttctc gccgaccgtg ggcctgggct atagcttcga acgccagcag
     2401 cttgcaggca accagggcgg caactcgccg ggcattcagg gcaacggcag cactatccag
     2461 acgacgcaga accccggcgg ccccgcgccc tacaacggcc ccgtcatcta caacttccac
     2521 acggcccagc tgaccgtggg ctacacgccc gatgtcttcg gcgccaaccg gcgccaggtc
     2581 gagtcgctgg aggcgcagaa ggatctggcc tattaccagc tgcaggccgc ttatctgacg
     2641 ctggcctcca acgtcgtcgc cgcggcgatc caggaggcct cgctgcgcgc gcagatagcg
     2701 cagaccgaaa gcatcatcgc cgccaacgag aagtcgctgc agatcctgca gcgccagctc
     2761 gcgaccggct atgcgatgcg cctggacgtg atggcccagg agacagcgct tgcccaggcg
     2821 cgtcagaccc tgccgccgct gcgcaagcag ctcgagcaga cgcgcgacct gatccgcatg
     2881 ctggccgggc agccgcccga cgccgaggtc gagcagcgct tcgagctggc ggacctgcac
     2941 ctgccgcagg agctgccggt gagcctgcct tccaagctgg tcgaccaccg ccccgacgtg
     3001 cgcgccgctg aagagcagct gcgctcggcc aatgcccagg tcggcgcggc cgtcgcggc
//
LOCUS       DASUCY010000553         2112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_269898, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000553 DASUCY010000000
VERSION     DASUCY010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269898"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VFV25_10885"
     CDS             <1..556
                     /locus_tag="VFV25_10885"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY1393875.1"
                     /translation="SGTVALYQITKQNVLTFDPVDTVFSIAVGEVQSKGVELDFSGEV
                     ATNLRLTAGYAFTDSKVTKSNAAAAGTGMAEGRRFPNVPRHSANLFAIYSLPVAGGSA
                     SIGAGLNFVGSRLGSVDVADNFTLPAYTTVKLVSSYDMSKHLRLSLDVDNLFNKACYA
                     SSYSDLWVFPGTERKFTLSALYKF"
     gene            576..1370
                     /locus_tag="VFV25_10890"
     CDS             576..1370
                     /locus_tag="VFV25_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393876.1"
                     /translation="MKRTLTAMAIGIASLLAPLAGQAHQIWFERDGSGITFRYGELDV
                     NMHEVSPGGLDRFKQLQATWLGARGEQPLALEKQRDRLTLPPGVKPAPGDSFIAVDKQ
                     YPMFDTQRDGKALRTWWTPATRWIGDFSARKPVLTLDVVPTGVKQEAGKVQFQVVYQG
                     EPLAGQKLNVATPAGWVRTVTSDTEGKFSVALPWRGNYAIGLYFVDDVSGERNNLDGK
                     AEKYQLEGYNTALSFYVARGLAPLPVADETLPASVRAERGLPPIKH"
     gene            1388..>2112
                     /locus_tag="VFV25_10895"
     CDS             1388..>2112
                     /locus_tag="VFV25_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HEY1393877.1"
                     /translation="MSSAAPIASPSRFANSVASTPAVAAHRSRITSIDALRGLVMLIM
                     LMDHVRETLYLHLQVTDPMDVAATPPQVFFSRLAAHFCAPVFVFLTGLGAWLYANPPS
                     GTPRPVRDFLLKRGLLMLVLEVTVVSFAWTGDMPLKVIYLQVIWAVGIAMIVLAFVHK
                     LSPRLLAAIGLVIVCGHNALAGINLPPDHPLYLPWTLLLHRGWALEDTAVKVRLTYPA
                     LPWVGVILLGWVAGPLYGAAFDSL"
BASE COUNT          339 a          734 c          689 g          350 t
ORIGIN      
        1 cagcggcacc gtggcgctct accagatcac caagcagaac gtgctcacct tcgacccggt
       61 cgatacggtg ttctccatcg cggtcggcga ggtccagagc aagggcgtcg agctggactt
      121 ctccggcgag gtggccacca acctgcggct gaccgcgggc tatgccttca ccgactcgaa
      181 ggtgaccaag agcaatgccg ccgcggccgg caccggcatg gcagagggcc ggcgcttccc
      241 caacgtgccg cgccacagcg ccaacctgtt cgcgatctat tcgctgccgg tcgcaggcgg
      301 cagcgcgagc atcggcgccg gcctgaactt cgtcggttcg cgcctgggct cggtggacgt
      361 ggccgacaac ttcacgctgc cggcctacac gacggtgaag ctggtgtctt cgtacgacat
      421 gagcaagcac ctgcgcctgt cgctcgacgt cgacaacctg ttcaacaagg cgtgctacgc
      481 cagctcctat agcgacctgt gggtgttccc cggcaccgag cgcaagttca cgctgagcgc
      541 gctgtacaag ttctgaacct cgaggagcac acaccatgaa acgcacactc accgccatgg
      601 ccatcggcat tgcatcgctg ctcgcgccct tggccggcca agcccatcag atctggttcg
      661 agcgcgacgg cagcggcatc accttccgct acggcgagct cgacgtcaac atgcacgagg
      721 tctcgcccgg cgggctcgat cgcttcaagc agctgcaggc cacgtggctc ggggcgcgcg
      781 gcgaacagcc gctcgcgctg gagaagcagc gcgaccgcct cacgctgccg cccggcgtga
      841 agcccgcgcc cggcgacagc ttcatcgccg tcgacaagca gtacccgatg ttcgacacgc
      901 aacgcgacgg caaggcgctg cgtacctggt ggaccccggc cacgcgctgg atcggagact
      961 tctcggcgcg caagccggtg ttgacactgg acgtcgtgcc caccggcgtg aagcaggagg
     1021 cgggaaaggt gcagtttcag gtggtctatc agggcgagcc gctggccggg cagaagctga
     1081 acgtggccac gcccgccggc tgggtgcgca ccgtaaccag cgacaccgaa ggcaagttca
     1141 gcgtcgcctt gccgtggcga ggcaactatg caatcggcct gtacttcgtg gacgacgtga
     1201 gcggcgagcg caacaacctc gacggcaagg ccgagaaata ccagctcgag ggctacaaca
     1261 ccgcgctgtc gttttacgtc gcgcgcgggc tggctccgct gccggtggcc gacgagacgc
     1321 tgccagcctc ggtgcgcgcc gagcgtgggt tgccgccgat caagcattga cccgacgagg
     1381 aggcctcatg tcttcagccg ctccaatcgc ctcgccatcg aggttcgcca actcagtggc
     1441 gagtacgccg gccgtggccg cgcaccgctc ccgcatcacg tcgatcgacg cgctgcgcgg
     1501 tctggtgatg ctgatcatgc tgatggacca cgtacgcgag acgctgtacc tgcacctgca
     1561 ggtcaccgac ccgatggacg tggccgccac gccgccgcag gtcttcttct cgcgcctagc
     1621 tgcgcatttc tgcgcgcctg tcttcgtgtt cctgaccggc ctgggcgcct ggctgtacgc
     1681 gaacccgccg agcggcacgc ccaggccggt gcgcgacttc ctgctcaagc gcggcctcct
     1741 gatgctggtg ctggaggtga cggtggtcag cttcgcctgg accggcgaca tgccgctgaa
     1801 ggtgatctac ctgcaggtga tctgggccgt cggcatcgcg atgatcgtgc tggccttcgt
     1861 gcacaagctg tcgcccaggc tgttggctgc gatcggcctc gtgatcgtgt gcggccacaa
     1921 cgcgctggcc ggcatcaacc tgccgcccga ccacccgctg tacctgccgt ggacgctgct
     1981 gctgcatcgc ggctgggcct tggaggacac ggccgtgaag gtgcgcctca cctatcccgc
     2041 gctgccctgg gtcggcgtga tcctgctggg ctgggtggcc ggccccctgt acggcgcggc
     2101 cttcgacagc ct
//
LOCUS       DASUCY010000554         3584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1121229, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000554 DASUCY010000000
VERSION     DASUCY010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1121229"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="VFV25_10900"
     CDS             complement(<1..813)
                     /locus_tag="VFV25_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEY1393878.1"
                     /translation="MTAPTTRVLTVLELLQARGSVGGAELAERLQVDRRTVRRYIEKL
                     GELGIPIAAERGRDGGYKLRPGFRLPPLMFTDDEALALSVGLLAARNLGLAEAAPAVA
                     SAQAKLERVTSNTLKRRIRALGDSIAIELTASPAGAGHDNAALVALSAAAQAQQRVQL
                     RYRSATGVDSLRDFDPYGLGYRGGRWYVVGHCHVRRGLRSFRLDRVLGVEALPASFLR
                     PEGFDILAYLANTIATLPRAFEVEVELEASLEQARAQLQATLGVLEALPQGGV"
     gene            913..1692
                     /locus_tag="VFV25_10905"
     CDS             913..1692
                     /locus_tag="VFV25_10905"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEY1393879.1"
                     /translation="MSSATDLALYFAIVFGIIVLPGMDMAFVLGNSLAGGRRAGMAAV
                     AGIIAGGLCHMGMGATGVSLLMRALPGLFTLMLLAGSLYVAWMGGSLLRASRGGATGG
                     SPALNSPAGAARPSSAFRGALLTSLLNPKAYLFTLAILPQFLHPQQGSIWLQVGALTL
                     ITSATQAGVYGTLALLASRSRAWLDANEAANRVVMRGVGVLLLAAAVFTGMEGLRALQ
                     GAASRPSAAAPGSNSAQATDEAPAGAPYGSRPGVRPWRKAS"
     gene            complement(1647..2141)
                     /locus_tag="VFV25_10910"
     CDS             complement(1647..2141)
                     /locus_tag="VFV25_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393880.1"
                     /translation="MPSALSLAARRVSQAWEPGLWIGALRLAFEAGPAVQQSDGGFAP
                     AYPAHYLVALWEPPASPLLPRWPAVAAIASPDADAALLELVSHLPAGQRVWLAREIID
                     WALVAEIVLRTDRNLAAYHRSELQGFIERCRASDAARIAAEYSDLDSGYEAFRQGLTP
                     GRDP"
     gene            complement(2180..>3584)
                     /locus_tag="VFV25_10915"
     CDS             complement(2180..>3584)
                     /locus_tag="VFV25_10915"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEY1393881.1"
                     /translation="LQGGQEQLLLSRYTGSRSSEWFLYLSAKGELKPLLGQGERNSYQ
                     LSFGPRADEYFVATSRFDDMTRLYRWHAARGTGEAAFERVAVGGTAAQRWEVEGFGID
                     FPKQRLYVSINEAGYTRSYALDARTLKPLALPFPKGLDHEALGAVSRDGRYVVLGYGW
                     PNTPRESVVYDWRSGKTTRWLMPSVPEQDLGRFVPATLESYPARDGTAIPMFVRRPPA
                     CAAAAAPCPVIVHFHGGPEGQSRPGFSPIWQFFVDAGFIIVEPNVRGSTGYGLAWLDA
                     DNGPKRLNIITDIEDAALYIRKAWAKNGAAPKIGVYGGSYGGYSTLYAMTRFAGAYDA
                     GVSNVGIANLLTFLQNTAPYRRALRASEYGDPVQDREALIQLSPTTYIDRLRSPLMVI
                     QGANDPRVPVGEALLMHDAAEKRGVPVELMIFADEGHGASSRANIALQYGHTLRFFQQ
                     HLMGGGANLAASAPAAQ"
BASE COUNT          544 a         1262 c         1239 g          539 t
ORIGIN      
        1 cacgccgccc tgcggcaggg cctcgagcac gcccagcgtg gcctgaagct gggcgcgcgc
       61 ctgctccagg ctggcttcca gctccacctc cacctcgaag gcgcgcggca gcgtggcgat
      121 ggtgttggcg agataggcca gtatgtcgaa gccctcgggc cgcagaaagc tggcgggcaa
      181 agcctccaca cccaacacgc ggtccagcct gaaggagcgc aagccgcggc gcacgtggca
      241 gtgtcccacc acataccagc gcccgccgcg gtagcccagc ccatagggat cgaagtcgcg
      301 cagcgagtca acgcccgtcg ccgagcggta gcgcagctgc acgcgctgct gagcctgcgc
      361 ggccgcgctc agcgccacca gcgcggcgtt gtcatggccg gcccccgcgg gcgacgcggt
      421 cagctcgatc gcaatgctgt cgcccagggc gcgtatgcgc cgcttcagcg tattcgacgt
      481 gacgcgctcc agcttggcct gggcgctggc cacggccggc gccgcctcgg ccagcccgag
      541 gttgcgcgcc gccagcaggc ccaccgacag ggccagggcc tcgtcgtcgg tgaacatcag
      601 cggcggcagc ctgaagccgg gcctcagctt gtagccgccg tcgcggccgc gctcggcggc
      661 gatcggtatg cccagttccc caagcttctc gatatagcga cgcaccgtgc ggcgatcgac
      721 ctgcagccgc tcggccagct ccgcaccgcc gacgctgccg cgcgcctgca gcagctccag
      781 cacggtcagc acgcgggtgg tgggggcggt catggcgctg cagtgtagat cgacgactat
      841 cgatataggt ctgtttctgc cctatatcac ctttaggctt gcgatcctct gcctccccca
      901 cagaagtccg caatgagcag cgcaaccgac ctcgccctct atttcgcgat cgtcttcggc
      961 atcatcgtgt tgccaggcat ggacatggcc ttcgtgctcg gcaactcgct ggccggcggc
     1021 cgccgcgccg gcatggccgc cgtggccggc atcatcgcgg gcggcctgtg ccacatgggc
     1081 atgggcgcca ccggcgtgag cctgctgatg cgcgcgctgc cggggctgtt cacgctgatg
     1141 ctgctggcgg gctcgctcta tgtcgcgtgg atgggcgggt cgctgctgcg cgccagccgc
     1201 ggtggtgcca ccggcggcag ccccgcgttg aacagccctg ctggcgcggc gcgccccagc
     1261 agcgccttcc gcggcgcctt gctgaccagc ctgctgaacc ccaaggccta tttgttcacg
     1321 ctggccatcc tgccgcaatt cctgcatccg cagcagggct cgatctggct gcaggtcggc
     1381 gcgctgacct tgatcaccag cgccacccag gccggcgtct acggcacgct ggcgctgctg
     1441 gcctcgcgca gccgggcctg gctcgatgcc aacgaagccg ccaaccgcgt cgtcatgcgt
     1501 ggcgtgggtg tcctgctgct ggctgccgcg gtcttcacgg gcatggaagg cttgcgcgcg
     1561 ctgcagggcg cggccagccg cccgtccgca gccgcgccgg gttcgaactc agcccaggca
     1621 acggacgaag cccctgctgg agcgccctat ggatcccgcc ccggcgtcag gccctggcga
     1681 aaagcctcgt agcccgagtc gagatcgctg tattcggccg cgatgcgtgc ggcgtcggag
     1741 gcccggcagc gctcgatgaa gccctgcagc tcgctgcggt ggtaggccgc cagattgcgg
     1801 tcggtgcgca gcacgatctc ggcgaccagg gcccaatcga tgatctccct ggcgagccag
     1861 acacgctgcc cggccgggag gtgcgaaacg agctccagca gggccgcatc ggcatcgggc
     1921 gatgcgatgg cggccaccgc cggccagcgc ggcagcagcg ggctggccgg cggctcccac
     1981 agtgccacga gatagtgcgc cgggtaggcg ggagcaaacc cgccgtcgct ctgctgcacc
     2041 gccggccccg cctcgaaggc caggcgcagc gcaccgatcc acaggcccgg ctcccaggcc
     2101 tgggagactc ggcgcgcggc aaggctcagc gcggatggca tgcttcgccc cagggctgca
     2161 gcctcctgcc tggatcgacc tattgagcgg ccggcgccga ggctgcgagg ttcgcgccgc
     2221 cacccatcag atgctgctgg aagaagcgca gcgtatgccc gtattgcagc gcaatgttgg
     2281 cgcggctgga agcgccatgc ccctcgtcgg cgaagatcat cagctccacc ggcacgccgc
     2341 gcttctccgc cgcatcatgc atcagcaagg cctcgccgac cggtacacgc ggatcgttgg
     2401 cgccctggat gaccatcaac ggcgagcgca gacgatcgat gtaggtggtc ggcgagagct
     2461 ggatcagtgc ctcgcgatcc tgcaccggat cgccgtactc gctggcgcgc aaggcgcggc
     2521 gatagggtgc cgtgttctgc aggaaggtca gcagattggc gatgcccaca ttggacacgc
     2581 ccgcgtcgta ggcgccggcg aagcgcgtca tcgcatacag cgtcgagtag ccgccatagc
     2641 tgccgccgta gacgccgatc ttgggcgcgg cgccgttctt ggcccaggcc ttgcgaatgt
     2701 agagcgccgc gtcctcgata tcggtgatga tgttcaggcg cttggggccg ttgtcggcat
     2761 cgagccaggc aaggccatag cccgtggagc cgcgcacatt gggctcgacg atgatgaagc
     2821 ccgcatcgac gaagaattgc cagatcggcg agaagccggg ccggctctgc ccctcgggcc
     2881 cgccgtggaa atgcacgatc accgggcaag gcgcggcggc tgcggcgcag gccggcgggc
     2941 ggcgcacgaa catcgggatg gccgtgccgt cgcgcgccgg atagctctcg agtgtggcag
     3001 gcacgaagcg gcccaggtcc tgctcgggca ccgagggcat cagccagcgc gtggtcttgc
     3061 cgctacgcca gtcgtagacg acgctctcgc gcggcgtatt gggccagccg tagcccagca
     3121 cgacatagcg accatcgcgc gagacggcgc cgagggcttc gtggtccaag cccttgggga
     3181 aaggcagcgc caggggcttg agcgtgcgcg catcgagcgc atagctgcgc gtgtagccgg
     3241 cctcgttgat gctcacgtag agccgctgct tggggaaatc gatgccgaag ccctcgacct
     3301 cccagcgctg ggccgcagtg ccgcctacag cgacgcgctc gaaagccgcc tcgcccgtgc
     3361 cgcgcgcggc atgccagcgg tatagccggg tcatgtcgtc gaagcgcgag gtcgcgacga
     3421 agtactcatc cgcgcgcggg ccgaagctca gttgatagct gttgcgttcg ccctggccca
     3481 gcaggggctt gagctcgccc ttggcggaca gatagaggaa ccactcggag ctgcgcgagc
     3541 ccgtgtacct gctcagcagc agttgctcct ggccgccttg caag
//
LOCUS       DASUCY010000555         3244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1041068, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000555 DASUCY010000000
VERSION     DASUCY010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041068"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VFV25_10920"
     CDS             <1..607
                     /locus_tag="VFV25_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEY1393882.1"
                     /translation="QFAGKNLLIVGGGDSALDWALNFAQEGPSKAESVILVHRRDGFR
                     AAPASVAKMKELCDAYEMQFIVGQVTGYEEKDGRLTGAKVTGGDGVTRVVPLDVLLVF
                     FGLSPKLGPIADWGLNLERKQIVVDTEKFETNVPGIFAVGDVNTYPGKKKLILSGFHE
                     AALAAFGAAPYIFPEKRIHLQYTTTSPKLHKVLGVETPVFD"
     regulatory      700..804
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            873..2984
                     /locus_tag="VFV25_10925"
     CDS             873..2984
                     /locus_tag="VFV25_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEY1393883.1"
                     /translation="MATPRSFAFKRIAFAAMQIAAAACATAQTTTLPTVTVTGRPANA
                     DVSGFGDVPPEKAPLQASVVSSEQLKDAGTQTLRALTRTDASVSDAYNSEGYWDNLTV
                     RGFVIDNRYNYRRDGLPINAETSIPLDNKARLELLKGTSGIQAGTSAPGGLVNLVVKR
                     PDTEVRSASLEWRSANTVTAAVDIGTRFGEGGAFGLRVNAAAAHLDSWLRSDKGERHL
                     FAVAGDWRLSPDTLLEAEFETSHRSQPSQPGFSMLGNVIPDARSIDPRINLNNQPWSQ
                     PVVLDGNSGSLRLQQRLSADWSARIHIGTQRLRSDDRVAFPFGCTAENNFTRYCSDGT
                     FDLYDYRSDGERRTTDALDMALQGRFDTGSVHHEFTGGVLLSRFTARFNPLIYNFAGT
                     GNIDGSLIVPPATSPTSDNTNRDERNTELYLRDAIRFTPEWSGWLGLRHTHLHRTSIG
                     TPSGQNPTDYGQSFSTPWLALSYEWQRGHMVYASWGEGVESQVVPGLSSYPNHGQALP
                     ALKSRQSEIGIKGKEELFSWTLTAFDIKQPAVTDTGTDFFIDGNEQHRGLEAGIDARF
                     GAWTWAAGAMLLRAERVGSTTIPDGYRPANVPAKALKLQTQYDVASLPGLALQAGLLA
                     QGDRMLRPDDNSLRVPGWTRIDLGERYEQHLTKGSLVWRVGVDNALNRRAWQEAPFQY
                     GHVYLFPVAPRTWRLSVEANL"
     gene            3021..3096
                     /locus_tag="VFV25_10930"
     tRNA            3021..3096
                     /locus_tag="VFV25_10930"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3054..3056,aa:Asn,seq:gtt)
BASE COUNT          616 a         1169 c          967 g          492 t
ORIGIN      
        1 gcagttcgcg ggcaagaacc tcttgatcgt cggcggcggc gactcggcac tcgactgggc
       61 gctcaacttc gctcaggaag gccccagcaa ggccgagagc gtgatcctcg tgcatcgccg
      121 cgacggcttc cgcgccgcgc ccgccagcgt ggccaagatg aaggagttgt gcgacgccta
      181 cgagatgcag ttcatcgtcg gccaggtcac cggctacgag gaaaaggacg gccgcctcac
      241 gggcgccaag gtcaccggcg gcgacggcgt gacccgcgtt gtgccgctcg acgtgctgct
      301 ggtcttcttc ggcctgagcc ccaagctcgg ccccatcgcc gactggggcc tgaacctgga
      361 gcgcaagcag atcgtcgtcg acaccgagaa gttcgagacc aacgtgccgg gcatcttcgc
      421 ggtgggcgac gtcaacactt accccggcaa gaagaagctc atcctctcgg gcttccacga
      481 ggccgcactg gccgccttcg gcgccgcgcc ctacatcttc ccggaaaagc gcatccacct
      541 gcagtacacg accaccagcc cgaagctgca caaggtgctg ggcgtcgaaa cccccgtctt
      601 cgactagccc ccgcctcctg tccgccaaga agcccgccct gcgcgggctt cttcgttggt
      661 ggctgcgcca taattcgctt gcaccgcgca gcccgctcgg tgcctcgcta ggggtgtcag
      721 gcctgtttct tcaggcccga ctgagagagt cccttcgaac ctgattgagg taatcctcgc
      781 gcagggaagc ggtctgaaag gcgcgcctcg tttccatccc tcttcttgag gccgcccacc
      841 gagagcgccc acctgccatc gggagcatca cgatggcaac accacgatca tttgcattca
      901 agcgcatcgc cttcgcggcc atgcagatcg ccgcggccgc atgcgccacg gcccagacca
      961 ccacgctgcc gaccgtcacc gtgacgggcc gcccggccaa cgcagacgtc tccggcttcg
     1021 gcgatgtccc gcccgagaag gcaccgctgc aggcgagcgt cgtttccagc gagcagctca
     1081 aggacgccgg cactcaaacg ctgcgcgcct tgacgcgcac cgatgccagc gtgagcgatg
     1141 cctacaacag cgaaggctat tgggacaacc tcacggtgcg cggcttcgtc atcgacaacc
     1201 gctacaacta ccggcgcgac ggcctgccca tcaacgccga gacctcgatc ccgctggaca
     1261 acaaggccag gctggaactg ctcaagggca cgagcggcat acaggccggc accagcgcgc
     1321 cgggcggcct cgtgaacctg gtcgtcaagc gccccgacac cgaggtgcgc agcgccagcc
     1381 tggaatggcg cagcgccaac accgtaaccg ccgcggtcga catcggcacc cgcttcggcg
     1441 agggcggcgc ctttggcctg cgcgtcaacg ccgcggcggc gcatctggac tcctggctcc
     1501 ggtccgacaa gggcgagcgc cacctgttcg ccgtcgccgg cgactggcgc ttgagccccg
     1561 acaccctgct cgaagccgag ttcgagacca gccatcgctc ccagcccagc cagcccggat
     1621 tcagcatgct gggcaacgtc atccccgatg cgcgcagcat cgatccgcgc atcaacctca
     1681 acaaccagcc ctggtcgcag cccgtggtgc tggacggcaa ctccggcagc ctgcgcttgc
     1741 agcagcgcct gagtgccgac tggagcgcca ggatccacat cggtacccag cgcctcagga
     1801 gcgacgatcg agttgccttc cccttcggct gcacagcgga gaacaacttc acgcgctact
     1861 gcagcgacgg cactttcgat ctctacgact accgcagcga cggcgagcgc agaaccaccg
     1921 atgcgctgga catggccctg cagggccgct tcgacaccgg cagcgtccat cacgagttca
     1981 ccggcggcgt gctgctgagc cgctttacgg cgcgcttcaa cccgctgatc tacaactttg
     2041 cgggcacagg caacatcgac ggcagcctca tcgttccgcc tgcaacctcg ccgacctcgg
     2101 acaacaccaa ccgcgacgaa cgcaacaccg agctctatct gcgcgatgcg atccgcttca
     2161 cgcccgaatg gagcggctgg ctgggactgc gtcacacgca tctgcatcgc accagcatcg
     2221 gcacgcccag cggccagaac cccaccgact acggacaaag cttcagcacg ccctggctgg
     2281 ccctgagcta cgaatggcag cgcgggcaca tggtctatgc gagctggggc gaaggcgtcg
     2341 aatcccaggt cgtgccgggc ttgagcagct accccaacca tggccaggcc ctgccggcac
     2401 tcaagagccg ccaaagcgaa atcggcatca agggcaagga agagttgttc agctggacgc
     2461 tgaccgcctt cgacatcaag cagcccgccg tcaccgacac cggcaccgat ttcttcattg
     2521 acggcaacga gcagcatcgc ggccttgaag ccggcatcga tgcgcgcttc ggcgcatgga
     2581 cctgggccgc cggcgcgatg ctgctgcgcg ccgagcgcgt cggcagcacc acgatcccag
     2641 acggctaccg ccccgccaac gtgcccgcga aggcgctcaa gctgcagacc cagtacgacg
     2701 ttgcaagcct gcccggcctg gccctgcagg ccggcctgct ggcccagggt gaccgcatgc
     2761 tcaggcccga cgacaacagc ctgcgcgttc ctggctggac acgcatcgac ctcggcgagc
     2821 gctacgaaca acacctgacc aaaggcagcc tcgtctggcg cgtcggcgtc gacaacgcgc
     2881 tcaaccgccg cgcctggcag gaagctccct tccagtacgg ccacgtctat ctcttcccgg
     2941 tggcgccgcg cacctggcgc ctgtcggtgg aggccaactt gtgaggcaac taaaaacccc
     3001 gctataatgt gaggctctgt tccccgatag ctcagtcggt agagcgccgg actgttaatc
     3061 cgtaggtccc tggttcgagc ccaggtcggg gagccaagcg aacagctaga agaatcaagg
     3121 ccttagcgcg atgcgccaag gccttttttc ttggccggat acccatagaa gttgtctttg
     3181 gcacaaatcc ggcacagtcg aagaatgcac gggccagaac ctgaaaggtc tgacctcatg
     3241 gcat
//
LOCUS       DASUCY010000556         2878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1313977, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000556 DASUCY010000000
VERSION     DASUCY010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1313977"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1490
                     /locus_tag="VFV25_10935"
     CDS             <1..1490
                     /locus_tag="VFV25_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEY1393884.1"
                     /translation="ASRALAGVDAPEVTVEVHLGPGLPAFHIVGLPDAEVREARDRVR
                     AALNHAHFEFPARRITVNLAPAELPKDSSRFDLPIALGILAASGQIDAAKLADHEFAG
                     ELSLAGDLRPVRGALALGLALRRSSSGGTPRALVLPEAALVQGLPLFGARHLLDVVRA
                     LQPGVAGEPLSRPRPQEPAARLNQLDLRDVKGQGAAKRALEIAAAGGHSVLMVGPPGG
                     GKSMLAQRFGGLLPPLSDDEALESAAVLSLTGGFDAATWKARTLRSPHHSASAAALVG
                     GGSPPRPGEISLAHHGVLFLDERPEFPRHALEALREPLETGRITISRAARQAEFPARF
                     QLLAAMNPCPCGHAGSPLRQCRCTPDAIARYQGKLSGPLLDRIDVQVEVPAVPPDALA
                     AAPKGESSTAVAERVARARERQQARQGHANAALDAAALDRHAALDTTASQFLQTAAAR
                     LGWSARGYHRVLRIARTVADLAGAPGVQLSHVAEAIQYRRVLSVQ"
     gene            complement(1539..2243)
                     /locus_tag="VFV25_10940"
     CDS             complement(1539..2243)
                     /locus_tag="VFV25_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HEY1393885.1"
                     /translation="MDSQQSNSTIPTAPQGLSALPRIVWVIGGALLVAIAGLASALVM
                     RSNNSEQSGQPEAAMQQQQQPGKAGTVAIAEPGHKAKSHNAGGYAGGGSANVPTTQVA
                     NACPNCGTVEGVQAVQQKGQGTGLGAVTGGVLGGVLGNQMGGGNGKTAMTVLGAVGGG
                     LAGNEVEKRARATTVYNVRVRMDDGSLRTMTLSHAPAPGERVAVEGQSLRVIGTSQGA
                     APQDAPRNYNTKGTGA"
     gene            complement(2419..>2878)
                     /locus_tag="VFV25_10945"
     CDS             complement(2419..>2878)
                     /locus_tag="VFV25_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009918375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HEY1393886.1"
                     /translation="EKKELQFVADLDDYSAITLKALADRLAEAFAERLHQRVRTELWG
                     YASDEQLSNEELIAEKYRGIRPAPGYPACPDHTVKREMFRVLACDEIGMGLTESLAMT
                     PAASVSGFYLAHPQAAYFNVGKIGADQLEDWARRTALSKAEAERALAPLL"
BASE COUNT          378 a         1027 c         1007 g          466 t
ORIGIN      
        1 tggcgagccg ggcgctcgcc ggcgtcgatg cgcccgaggt gaccgtcgaa gtccatctcg
       61 gtccaggact gccggcgttt catatcgtgg ggctgccgga tgcagaagtg cgcgaagcgc
      121 gcgatcgcgt gcgtgccgcg ctcaaccacg cgcacttcga gtttccggcg cgccgcatca
      181 cggtcaatct cgcgcccgcg gaactgccga aggattcgag ccgtttcgat ctgccgatcg
      241 cgctcggcat cctggctgcg agcgggcaga tcgatgcggc caagctggcc gaccacgaat
      301 tcgccggcga gctatcgctg gccggcgatc tgcgcccggt gcgcggcgca ctcgccctgg
      361 gcctggcgct gaggcgcagc agcagcggcg gcacgccgcg cgcgctggta ttgcccgagg
      421 ccgcgctggt ccagggcctg cccctcttcg gtgcacggca cctgctcgat gtggtgcgcg
      481 cactgcaacc gggcgtggca ggcgagccgc tgtcgcgccc ccggccacag gagccggccg
      541 cgcgcctgaa ccagctcgac ctgcgcgacg tcaagggcca gggtgccgcg aagcgggccc
      601 tggagatcgc cgcggcgggc ggccacagcg tgctgatggt ggggccgccc ggcggtggca
      661 agtccatgct ggcgcaacgc ttcggcggcc tgctgccgcc gctcagcgac gacgaggcgc
      721 tggaatcggc tgccgtgctc agcctgaccg gcggcttcga cgcggcaacc tggaaggcgc
      781 gcacactgcg ctcgcctcac cactcggcct cggccgcggc gctggtgggc ggaggctcgc
      841 cgccgcggcc aggcgaaatc tcgctggccc atcacggcgt gctcttcctc gacgaaaggc
      901 ccgagttccc tcgccatgcg ctggaggctc tgcgcgagcc gctggagacc ggccgcatca
      961 cgatctcgcg cgcggcgcgc caggctgaat tcccggcacg cttccagctc ctcgccgcaa
     1021 tgaacccctg cccttgcggc catgccggca gccccttgcg gcaatgccgc tgcacgcctg
     1081 acgcgatcgc gcgctaccag ggcaagctca gtgggccgct gctcgatcgc atcgatgtgc
     1141 aggtggaagt gccggccgtg ccgcccgacg ccctggccgc ggcgcccaag ggtgaatcca
     1201 gcaccgctgt ggccgagcgc gtcgcacggg cgcgcgagcg ccagcaggca cgccagggcc
     1261 atgccaatgc cgcgctggac gccgcggccc tggaccgcca tgcagccctg gacaccaccg
     1321 cgagccaatt cctgcagacc gccgccgcgc gcctgggctg gtcggcccgt ggctaccacc
     1381 gcgtgctgcg catcgctcgc accgtggcgg atctggctgg cgcaccgggc gtgcagctct
     1441 cgcatgtggc cgaggcgatc cagtaccggc gcgttctgag cgtccaatga aaatggcccg
     1501 ccgaagcggg ccatgcggga gagctaagtc ctgattggtc aggcgcccgt tcccttggtg
     1561 ttgtagttgc ggggcgcgtc ctgcggggcg gcgccctggg acgtgccgat cacgcgcagg
     1621 ctctggcctt cgaccgcgac gcgctcgccg ggcgccggcg catggctcag cgtcatggtg
     1681 cgcagcgaac cgtcgtccat gcgcacgcgc acgttgtaga ccgtggtggc gcgggcgcgc
     1741 ttctcgactt cgttaccggc caggccgccg cccacggcgc ccagcaccgt catcgcggtc
     1801 ttgccgttgc cgccgcccat ctggttgccc agcacgccgc ccagcacgcc gccggtgacg
     1861 gcgccgaggc cagtgccctg gcccttctgc tgcacggcct gcacgccttc aacggtgccg
     1921 cagttcgggc aggcgttggc gacctgggtg gtcggcacgt tcgcgctgcc gccacccgca
     1981 tagccacccg cgttatggga cttggccttg tggcccggct cggctatcgc aacggtgccc
     2041 gccttgcccg gctgctgttg ttgctgcatg gccgcctcgg gctggccgct ctgctcggaa
     2101 ttgttgctac gcatcaccag cgcggaggcc aggcctgcga tggcgaccag cagggcgccg
     2161 ccgatcaccc agacgatgcg cggcaaggcg ctgagaccct ggggcgcagt ggggatggtg
     2221 ctattggatt gttggctgtc catgctcgga ctcctttcct cgtgtgttta tcgggcatgc
     2281 gctcccgccg tggcgggcag cgcctacctc tattcaacgc tgcagaagcg aaatggtcgg
     2341 cattggcgtc gttggacttg taacagcagg ttagtgttcc ctgcccggtt cacgtcctga
     2401 aacggctttg cggcggcctc acaacagggg cgccagcgcg cgttccgcct ccgccttcga
     2461 taaggcggtg cggcgcgccc agtcctcgag ctggtcggcg ccgatcttgc cgacgttgaa
     2521 ataggccgcc tgcggatgcg caagataaaa gccgctgacg ctggcggccg gcgtcatcgc
     2581 caggctctcg gtcaggccca tgccgatctc gtcgcaggcc agcacgcgga acatctcgcg
     2641 cttgacggta tggtccgggc aggcggggta gccgggcgcc gggcggatgc cgcggtattt
     2701 ctcggcgatc agctcctcgt tgctgagttg ctcgtcactc gcatagcccc acagctcggt
     2761 gcgcacgcgc tggtgcagcc gttcggcgaa ggcctcggcc aggcggtcgg ccagggcctt
     2821 gagggtgatg gccgagtagt cgtccaggtc ggcaacgaac tgcagctcct tcttctcc
//
LOCUS       DASUCY010000557        12288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_398264, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000557 DASUCY010000000
VERSION     DASUCY010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 12288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12288
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398264"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /gene="rseP"
                     /locus_tag="VFV25_10950"
     CDS             complement(<1..1171)
                     /gene="rseP"
                     /locus_tag="VFV25_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEY1393887.1"
                     /translation="MIITVLAFLLTLGVLILVHEYGHYRVAVACGVKVQRFSIGFGRA
                     LWRGRRGETEFVLGLLPLGGYVKMLDEREGDVPAHELDRAFNRKPLWQRAAVVVAGPT
                     ANLLLAWLLFAAASWWGVSEPKALLGSPLPASLAERAGQRAGDLVQAVSTDGTSWTDV
                     ASYDDLIQQVAQAVLRGETLHLSLADAQGRGPRESELPLATLGAREVDAALVKRIGLG
                     QPYREPVMADVLPGGPAAKAGLRRGDRVLSVDGIAVADAGQLHEMVRASARDGQAHAM
                     NWHIERNEHVLDVSVTPEVAKDKERAVGRIEAYLGGPVEMVTVQRGPVEGLVRAGVRT
                     WDTAKLNLQLLGKMLIGKASLKNLSGPLTIADYAGQSVQRGLAYYLGFLAIVSVGL"
     gene            complement(1168..2382)
                     /gene="ispC"
                     /locus_tag="VFV25_10955"
     CDS             complement(1168..2382)
                     /gene="ispC"
                     /locus_tag="VFV25_10955"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEY1393888.1"
                     /translation="MSKPSSSPSERQVQRVCVLGSTGSIGANTLDVIARHPQRYEVFA
                     LTANSRVDLLLEQCLRWRPRHAVLPDAKLAADLQDRLRREGLTTEVRSGASALVEVAA
                     HPEVEILMAAIVGAAGLEPCLAAARAGKRLLLANKEALVVGGAVFMQAVHEGGATLLP
                     IDSEHSAIFQCLPEDRASWPQRIDHIVLTASGGPFRTRDPRTLAKVTPDEACAHPNWV
                     MGRKISVDSATMMNKALEVIEARWLFDLRPEQIRVVIHPQSIIHSMVVCRDNSVLAQL
                     GTPDMRVPIAYGLAWPERIESGASSLDFLSLAALSFEAADALRYPGLQLAWDTLRGPA
                     GSTAVLNAANEVAVAAFLAGTIGFDRIHTVNARTLDGFDFGTGHAASLEELLAVDARA
                     RQTAGQIVKALA"
     gene            complement(2386..3222)
                     /locus_tag="VFV25_10960"
     CDS             complement(2386..3222)
                     /locus_tag="VFV25_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEY1393889.1"
                     /translation="MLRQRVITAVVLLALLLPALLAHSLWPFAMLCLLAVGAAGWEWG
                     RLNGATSASALALGALLVIGGALAYSSHAAATSGWLWWAAVSVWVLGGVWALGRGVAA
                     WPRLGKGLRAVVGLAILLTAWLALVRAKAMGVNFLLSVFCLVWAADIAAYFGGRAFGR
                     HKLAPSISPGKSWEGVWSGMLGVLLLACAWLALDSRGAMVDSQSIYSLLRERAGWAGL
                     TVGVLFLTAMSVVGDLFESMVKRSAGAKDSSQLLPGHGGVLDRIDALLPVFPIALALA
                     SF"
     gene            complement(3226..3963)
                     /gene="uppS"
                     /locus_tag="VFV25_10965"
     CDS             complement(3226..3963)
                     /gene="uppS"
                     /locus_tag="VFV25_10965"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEY1393890.1"
                     /translation="MDQQQSAAVPRHIAIVMDGNGRWAKKRFMPRFFGHKHGVDALVR
                     IVKACADRGVEYLTVFAFSSENWKRPEDEVSGLMGLVLVAVANHLSKLASEGVRIRIV
                     GDRAAVSDKVRAAWDEAESATRHNTRITLSVAFNYGGRWDIVQACRCAIEAGVPGDQL
                     DEAKLASYMALSHAPDPDLFIRTGGEVRISNFLLWQLAYSELHFTDCLWPDFGEAEVD
                     AAIAAFAGRERRFGGVLSEQHAVANSR"
     gene            complement(3997..4557)
                     /gene="frr"
                     /locus_tag="VFV25_10970"
     CDS             complement(3997..4557)
                     /gene="frr"
                     /locus_tag="VFV25_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEY1393891.1"
                     /translation="MSTADIKKTTEQKMLKSVEAFKTELTKIRTGRAHAGLLDQVHLE
                     YYGSMVPISQVANVTLLDARTISVQPWEKGMGAKIEKAIRESDLGLNPASQGDLIRVP
                     MPPLTEERRRELTKVVKHTGEEAKVAVRNLRRDANEQVKKLLKDKQVTEDEERRAQDE
                     VQKLTDRMIAEIDRLIQAKDAEILAV"
     gene            complement(4591..5301)
                     /gene="pyrH"
                     /locus_tag="VFV25_10975"
     CDS             complement(4591..5301)
                     /gene="pyrH"
                     /locus_tag="VFV25_10975"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEY1393892.1"
                     /translation="MPAYKRILLKLSGEALMGDDAYGINRATMVRMVQEVQEVTRLGC
                     EVAVVIGGGNIFRGVAGGSVGMDRATADYMGMLATVMNALALADTMRQEGMVARVMSA
                     ISIEQIVEPYVRPKALQYLEEGKVIVFAAGTGNPFFTTDTAAALRGAEIGAEVVLKAT
                     KVDGVYTADPKKDPEARRYSRISFDEAIVRNLQVLDATAFALCRDQKLPIKVFSIFKP
                     GALKRVVMGEDEGTLVHV"
     gene            complement(5365..6255)
                     /gene="tsf"
                     /locus_tag="VFV25_10980"
     CDS             complement(5365..6255)
                     /gene="tsf"
                     /locus_tag="VFV25_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEY1393893.1"
                     /translation="MAAITASMVAELRAKTDAPMMECKKALTEAAGDMAKAEDLLRVR
                     LGSKASKAASRVTAEGIVAAYIDGNTGALIELNCETDFVSKNDAFLTLGKALAELVAK
                     HNPADVAALSALPLTQGDFGPTVEDVRKGLIGKIGENMSIRRFKRFAGDSKLVSYLHG
                     TRIGVVVEYSGDEVAAKDVAMHVAAMKPVSLSAAEVPAELIEKERSIATAKAAESGKP
                     ADIVAKMVEGSVQKYLKEVSLFNQAFVKNDKQTVEQMLKAVNTSVKGFTLYVVGEGIE
                     KKQDDFAAEVAAQVAAARGQ"
     gene            complement(6377..7120)
                     /gene="rpsB"
                     /locus_tag="VFV25_10985"
     CDS             complement(6377..7120)
                     /gene="rpsB"
                     /locus_tag="VFV25_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEY1393894.1"
                     /translation="MSFTMREMLEAGVHFGHQTRFWNPKMAPYIFGHRNKIHIINLEK
                     TLPLFQDAMKFARQLASKRGTILMVGTKRQAREVVAQEAQRAGMPYVDQRWLGGMLTN
                     FKTVKGSLKKLKDMQAQAEAGLDQLSKKEGLLFQREIAKLEKDIGGIQDMNALPDALF
                     VIDVGYHKIAVAEAQKLGIPVIGVVDSNHSPEGIDYVIPGNDDSAKAVALYARAIADA
                     VLEGKANAVNEVVHAAQSGDEFVEVSDAA"
     gene            7303..8664
                     /locus_tag="VFV25_10990"
     CDS             7303..8664
                     /locus_tag="VFV25_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEY1393895.1"
                     /translation="MADDLIAARELVRSGRSSATALLDDCLAAAASPEARHAFIELKP
                     DDARVAARSTDAARAAGHPLPPLAGLAVSVKDLFDVQGQVTRAGSKVLHDAPPALADA
                     PAVARLREAGAALIGRSNMTEFAFSGVGINPHYGTPANVAMGRLDAAAPRIPGGSTSG
                     GAVSVACGAAFAALGSDTGGSIRIPAALQGLVGYKNTAALTPTAGALPLSTTLDTACA
                     ITRSVRDAVLLHEVLAQRSVRLAGRPLKGTRFAVAGTLMQDALEPAVALAFERALKAL
                     AASGAAIEDLPLPMLGQLAAMQSRGSFSAAEAWTWHRSLLEARGADYDHRVAQRICTG
                     AAMTAADYIELQRARRDWIARMETALLGYDAVLSPTVPILAPPIAALEADDAAFFRAN
                     AALLRNTSVANMLDGCAISLPCQARGEPPVGLMLWAAGGRDDALLDAALAVEAALAAA
                     LDA"
     gene            8661..10037
                     /locus_tag="VFV25_10995"
     CDS             8661..10037
                     /locus_tag="VFV25_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY1393896.1"
                     /translation="MRVAVVGAGIAGVTTAYELAADGHEVAVFERRGSVAAEGSFANA
                     GVLAYHYAAQWLSAEPRSILLGRLFGNRTALSWCPSWRAAQWRRLSQLGRGDGRAAQQ
                     RAADLHALARFSVERLQTLTAQLQLEYEQGAGYLVLLRRERELARLRTELAALRELNL
                     SYRELDPAQCRALEPGLNPDQPLAGGIRFEQGQVANCRQFAHLLREAAERRGAEFHFG
                     AAATLLGADAGGPMLRVEQHALSTGFAATTLAHGKDAVQVRRQVMQRRSAAAARYLDP
                     VSEQRYDAVVLCAGAASATMLRTIGLRLPLVPVHGYSMTAPLRAPERAPCSALMDEQY
                     KVVISRMGQRVRVAGGAEIGGHPAKHDRAAIATLYRVLGDWFPGAAHLARPQLWKGAR
                     AMLPGGLPLIGASGAPGVWLNLGHGAGGWTLSCGSARLLADLMGGHPTAVDARGYSFE
                     RYDKRQGR"
     gene            10100..11644
                     /locus_tag="VFV25_11000"
     CDS             10100..11644
                     /locus_tag="VFV25_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEY1393897.1"
                     /translation="MSPLQLVRVLPELAQRVPSLGAWPLHDAAATRELETRAAAALPS
                     HVLMQRAGLAVARLAQAVAPHARRVWIAAGPGNNGGDGLEAAMHLHLAGREVSVSLAA
                     DGARLPADAAASLQRAKAAGVRITDAIEAPPPTPDALAIDALLGLGASRPPGGNIAQA
                     TDALNRGALPVLAIDLPSGLNASTGQAFEACVRARWTLSLLTLKPGLFTSAGRDHAGE
                     VWFDSLGVAGNADAGCAWLPCRETLLGLGPRRAHSQHKGSFGDVWIVGGAPSMVGAAL
                     LAARASLYAGAGRVYLHLLDASAARFDPAQPELMLRDTLPDAAALPGCTVVCGCGGGE
                     AVAAHLPGLLVRAGRLLLDADALNAVAADAALAQRLSQRAAHGLATLLTPHPLEAARL
                     LKQGAADVQADRLAAARALAQRYQSVVVLKGSGSVICAPGAKPSVNPSGNAALASPGT
                     GDVLAGWLGGLWSQGLSAHDAARLGTYQHGAAADGWLAERGAALALPAGALVQRLALL
                     PQPTAT"
     gene            complement(11670..>12288)
                     /locus_tag="VFV25_11005"
     CDS             complement(11670..>12288)
                     /locus_tag="VFV25_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEY1393898.1"
                     /translation="EAAQWETAGIHCSVNERRADEASRDVEAWLKCRFMREHLGEEFS
                     GTVSAVTSFGLFVQLDNLYVEGLVHITELGGEYFRFDEARQELRGERTGVRYAVGTRV
                     RVQVSRVDLDGRKIDFRLVREGGAAPRSHRGAADKAGSAVDALKGVQHADRALKQSAK
                     AKKSSAGKKQAAAKTQVSAVQPDKVEAAQRAQVLRAERKGKRARR"
BASE COUNT         1836 a         4320 c         4134 g         1998 t
ORIGIN      
        1 ccagcccgac gctgacgatc gcgagaaagc cgagatagta ggccaggccg cgctgcaccg
       61 attggccggc gtagtcggca atcgtcagcg gcccgctcag attcttgagc gaggccttgc
      121 cgatcagcat cttgcccagc agctgcaggt tgagcttggc ggtgtcccag gtccgcaccc
      181 cggcgcggac tagcccctcg acaggaccgc gctgcaccgt gaccatctcg accggcccgc
      241 ccagataggc ctcgatgcgc ccgacggcgc gctccttgtc cttcgcgacc tctggggtga
      301 cgctcacgtc aagcacatgc tcattacgct cgatgtgcca gttcatcgca tgggcctggc
      361 cgtcgcgcgc cgaggcccgc accatctcgt gcaattgccc ggcatcggcg acggcgatgc
      421 catccacgct cagcacccga tcgccgcgtc gcagcccggc cttggcagct gggccgccag
      481 gcagtacatc ggccatcacc ggctcgcgat aaggctgccc caggccgatg cgcttgacga
      541 gcgcggcatc gacttcgcgc gcgcccagag tagcgagcgg cagctcggac tcgcgcgggc
      601 ccctgccctg cgcatcggcc agcgacaggt gcagcgtctc gccgcgcagc acggcctgcg
      661 cgacctgctg gatcaggtcg tcgtacgacg caacatctgt ccagctcgtg ccatccgtcg
      721 acaccgcctg caccagatcg ccggcgcgct ggccggcgcg ctcggccagg ctggccggaa
      781 gcggactgcc aagcaaagcc ttgggttcac tgacgcccca ccaggaggcg gcggcaaaca
      841 gcagccaggc aagcagcagg ttggccgtcg gcccggccac cacgacagcg gcgcgttgcc
      901 acagcggctt gcggttgaag gcgcggtcga gctcgtgcgc cggcacgtcg ccctcgcgct
      961 cgtcgagcat cttgacgtag ccgcccagcg gcagcagccc cagcacgaat tcggtttcgc
     1021 cgcgccggcc gcgccacagc gcgcggccga agccgatcga gaagcgctgc accttgacgc
     1081 cgcaggccac ggcgacgcgg tagtggccgt attcgtgcac caggatgagc acgcccaggg
     1141 tcagcagaaa ggcgaggacg gtgatgatca tgcgagggcc ttaacaatct gcccggcggt
     1201 ctggcgagcc cgcgcatcga cggcaagcag ctcttccaga ctggcggcgt ggcccgtacc
     1261 gaagtcgaag ccgtccaggg tgcgggcgtt gaccgtgtga atgcggtcga agcctatggt
     1321 tcccgccagg aaggccgcca ccgcgacctc attggctgca ttcagcacgg ccgtgctgcc
     1381 ggccggtccg cgcagggtgt cccaggccag ctgcaggccg ggatagcgca gggcatcggc
     1441 cgcctcgaag ctcagcgcgg ccagcgacaa aaaatccagg ctcgaggcgc ccgactcgat
     1501 gcgctcgggc caggccaggc cgtaggcgat gggcacgcgc atgtcgggcg tgccgagctg
     1561 ggccagcacc gagttgtcgc ggcacacgac catcgagtgg atgatgctct gcggatggat
     1621 cacgacgcgg atctgctcgg gccggaggtc gaacagccag cgcgcctcga tcacctccag
     1681 cgccttgttc atcatggtgg ccgaatcgac cgagatcttg cgccccatga cccagttggg
     1741 atgcgcgcag gcctcgtccg gcgtcacctt ggcgagcgtg cgcggatcgc gtgtgcggaa
     1801 gggcccgccc gaggcggtca gcacgatatg gtcgatacgc tgcggccagc tcgcgcgatc
     1861 ctcgggcagg cattggaaga ttgccgaatg ctcgctgtcg atgggcagca gcgtcgcgcc
     1921 gccctcgtgc actgcctgca tgaacaccgc gccgcccacc accagcgcct ccttgttggc
     1981 cagcagcagg cgcttgccgg cgcgagccgc ggccaggcag ggctcgagcc cggcggcgcc
     2041 gacgatggcg gccatcagga tctcgacttc ggggtgggcc gccacctcca ccagggcgct
     2101 ggcgccgctg cgcacttcgg tggtcaagcc ttcgcggcgc aggcggtcct gcagatccgc
     2161 cgccagcttg gcatcaggca ggacggcgtg gcgcggccgc cagcgcaggc actgctcgag
     2221 caggaggtcg acgcggctgt tggccgtgag cgcgaagact tcgtagcgct gcgggtggcg
     2281 cgcgatgacg tccagcgtgt tggcgccgat cgagccggtg gagcccagca cgcagacacg
     2341 ctgaacctgg cgctcggaag gtgatgatga aggcttgctc atcgctcaga aggatgcgag
     2401 cgccagtgcg atcgggaaca ccggcagcaa ggcatcgatg cggtccagca cgccgccatg
     2461 gccgggcagc agctggctgc tgtccttggc cccggcgctg cgcttgacca tggattcaaa
     2521 cagatcgccc accacgctca tcgcggtaag gaagagtaca ccgacagtca gcccggccca
     2581 gccggcccgc tcgcgcagaa ggctgtagat gctctgcgag tcgaccatgg cgccgcggct
     2641 gtccagcgcc agccaggcgc aggcgagcag cagcacgccc agcatgccgc tccacacccc
     2701 ttcccagctc ttgccggggc tgatggaagg cgccagcttg tgccggccga aggcgcgccc
     2761 gccgaagtag gcggcgatgt cggcggccca cacgaggcag aacaccgaca gcaggaagtt
     2821 cacgcccatc gccttggccc gcaccagggc cagccaggcc gtgagcagga tggccaggcc
     2881 gacgacggca cgcagcccct ttcccagcct cggccaggca gcaactccgc ggcccagggc
     2941 ccagacgcca cccagcaccc agaccgaaac ggcggcccac cacagccagc ctgacgtcgc
     3001 ggcggcgtga ctcgaatagg ccagcgcccc gccgatcacg agcagagccc ccagcgccag
     3061 cgcggaagca ctggtcgcac cgttgagccg gccccattcc cagccggccg cgccgaccgc
     3121 cagcaggcaa agcattgcaa agggccacag gctgtgggct aacagggccg gcagcagcaa
     3181 ggccagcagc acgaccgctg tgatgacgcg ctgccttagc atgattcaac ggctgttcgc
     3241 caccgcgtgc tgctcggaca gcaccccgcc gaagcgccgc tcgcgccccg caaacgcggc
     3301 gatggcggcg tcgacctcgg cctcgccgaa atcgggccac aggcagtcgg tgaaatgcag
     3361 ctcgctatag gccagctgcc acagcaggaa gttggagatc cgcacctcgc caccggtgcg
     3421 gatgaagaga tcgggatcgg gcgcgtgact gagcgccatg tagctggcga gtttggcctc
     3481 gtcgagctgg tcgccgggca cgccggcctc gatggcgcag cggcaagcct gcacgatgtc
     3541 ccagcggccg ccgtagttga aggcgaccga cagcgtgatg cgggtgttgt ggcgcgtggc
     3601 gctttctgcc tcgtcccagg cggcgcgcac cttgtccgac accgcggcac ggtcgccgac
     3661 gatgcgtatg cgcacgccct cgctggcgag cttgctcagg tggttggcca ccgcgaccag
     3721 cacgagcccc atcaggcccg agacctcgtc ctccggccgc ttccagttct cggaggaaaa
     3781 ggcgaacacg gtgaggtatt ccacgccgcg atcggcgcag gccttgacga tgcgcaccag
     3841 ggcatcgacg ccatgcttgt ggccgaagaa gcgcggcatg aagcgcttct tggcccaacg
     3901 gccgttgccg tccatcacga tcgcgatatg gcgcggcacg gccgcactct gctgctgatc
     3961 cacagccttg ccccgctgaa gaaaactgag cacgactcag acggccagga tctcggcgtc
     4021 cttggcctgg atcagccgat cgatctcggc aatcatgcga tcggtcagct tctgcacctc
     4081 gtcctgagcg cggcgctcct cgtcctcggt gacctgcttg tccttgagca gcttcttgac
     4141 ctgctcgttg gcgtcgcggc gcagattgcg caccgccacc ttggcctcct cgccggtgtg
     4201 cttcacgacc ttggtcagct cccggcggcg ctcttcggtc agcggcggca tcggcacgcg
     4261 gatcaggtcg ccctgcgaag ccggattgag tccgaggtcg gattcgcgga tcgccttctc
     4321 gatcttggcg cccatgccct tctcccaggg ctgcacgctg atggtgcgcg cgtcgagcag
     4381 ggttacgttg gcgacctggc tgatcggcac catggagccg tagtactcga ggtgcacctg
     4441 gtcgagcagg ccggcgtggg cgcggccggt gcggatcttg gtcagctcgg tcttgaacgc
     4501 ctcgaccgac ttgagcatct tctgctcggt cgtcttcttg atgtctgcag tgctcatggc
     4561 atcttcctca acaaggctgc tcgcacgcga tcagacgtgg accagcgtac cttcatcttc
     4621 gcccatcacg acgcgcttca gcgcgcccgg cttgaagatg ctgaagacct tgatcggcag
     4681 cttctggtcg cggcacagcg caaaggccgt cgcgtccagc acctggagat tcctgacgat
     4741 ggcctcgtcg aagctgatgc ggctgtagcg cctggcctcc gggtccttct tggggtcggc
     4801 ggtgtagacg ccatccacct tggtcgcctt cagcaccacc tcggcgccga tctccgcgcc
     4861 gcgcagcgcg gccgccgtgt cggtcgtgaa gaagggatta ccggtgccgg ccgcgaacac
     4921 gatcaccttg ccctcttcga gatactgcag ggccttgggc cgcacatagg gctcgacgat
     4981 ctgctcgatg ctgatggccg acatgacgcg tgcgaccatg ccttcctgcc gcatggtgtc
     5041 ggccagggcc agcgcgttca tcacggtggc cagcataccc atgtagtcgg ctgtggcccg
     5101 gtccatgccg accgagccac cggccacacc gcggaagatg ttgcccccgc cgatcaccac
     5161 cgccacttcg cagccgagcc gggtcacttc ctgcacttcc tgcaccatgc gcaccatggt
     5221 ggcgcggttg atgccgtagg catcgtcgcc catcagcgcc tcgccggaga gtttgagcag
     5281 gatgcgtttg tacgccggca tgcgggggtc tcctggggtc aaggcggtgc ggaagtgtag
     5341 gtgatccggg taaaggactt ttgcttactg gccccgggcg gcggccacct gggcagccac
     5401 ttcggcggcg aagtcgtcct gcttcttctc gatgccctcg cccaccacgt agagcgtgaa
     5461 gcccttcacg ctcgtgttga cggccttgag catctgctcg acggtctgct tgtcgttctt
     5521 cacgaaggcc tggttgaaca gcgagacctc cttcaggtac ttctgcaccg agccttcgac
     5581 catcttggcg acgatgtccg ccggcttgcc cgactcggcc gccttcgcgg tcgcgatcga
     5641 gcgttccttt tcgatcagct cggccggcac ttcggcggcc gacagcgaca ccggcttcat
     5701 cgcggccacg tgcatggcca cgtccttggc cgcgacttcg tcgccgctgt actccaccac
     5761 cacgccgatg cgcgtgccgt gcagatagct caccagcttg ctgtcgccgg cgaagcgctt
     5821 gaagcggcga atgctcatgt tctcgccgat cttgccgatc aggcccttgc gcacgtcctc
     5881 gacggtgggg ccgaagtcgc cctgcgtcag cggcagggcc gacagcgccg ccacgtcggc
     5941 cgggttgtgc ttggcgacca gctcggccag cgccttgccc agcgtaagga aagcgtcgtt
     6001 cttggagacg aaatcggtct cgcagttcag ctcgatcaag gcgccggtgt tgccgtcgat
     6061 gtaggcggca acgatgccct cggcggtcac gcgcgaggcg gccttgctgg ccttgctgcc
     6121 gagcctgaca cgcagcaggt cctcggcctt ggccatgtcg cctgcggcct cggtcaaggc
     6181 cttcttgcat tccatcatcg gggcgtcggt cttggcgcgc agttcggcga ccatgctggc
     6241 ggtgattgca gccattgctc tttctccgtt cgttccatcg gctgcctcgg ggcagccgtc
     6301 attgaagctg tgttgaaaaa aaggggctga atcagccccc ttctccttgc gctgtgccgg
     6361 cgcgtgctct cgaggttcag gcggcgtcgc tgacttccac gaactcgtcg ccgctctggg
     6421 cggcatgcac gacctcgttc acggcgttgg ccttgccttc cagcacggcg tcggcaatgg
     6481 cgcgcgcata cagggccacg gccttggccg agtcgtcatt gccggggatc acgtagtcga
     6541 tgccttcggg cgaatggttg gaatccacta cgccgatcac cggaatgccc agcttctggg
     6601 cctcggcaac ggcgatcttg tgatagccga cgtcgatcac gaacagcgca tcgggcagcg
     6661 cgttcatgtc ctggatgccg ccgatgtcct tctcgagctt ggcgatctcg cgctggaaca
     6721 gcaggccttc cttcttgctc agctgatcca ggccggcttc ggcctgcgcc tgcatgtcct
     6781 tgagcttctt cagcgagccc ttgaccgtct tgaagttggt cagcatgccg cccagccagc
     6841 gctggtcgac atagggcatg ccggcgcgct gcgcttcctg tgccacgact tcacgggcct
     6901 ggcgcttggt accgaccatc aggatggtgc cgcgcttgga cgccagctgg cgggcgaact
     6961 tcatcgcatc ctggaagagc ggcagcgtct tctcgaggtt gatgatgtgg atcttgttgc
     7021 gatggccgaa gatatagggg gccatcttgg ggttccagaa gcgggtctgg tgtccgaaat
     7081 ggacgcccgc ttccagcatt tcacgcatgg tgaaggacat ggatgctcca aaaaggttgt
     7141 gtctagaatc cggcctgaat tgcaagcggc tgctttcttt gcaagccact gcaacacctt
     7201 cgggtggccg gattcgcgtc tgtgtctacc cgcagcaggc gagccaccgg gaaacccagg
     7261 cgcgcgccgc atcgagataa accgaaaaat tatagcacgc caatggccga cgacctgatc
     7321 gctgcccgtg aactcgtgcg cagcggccga agctcggcga ctgcgctgct cgacgactgc
     7381 ctcgcagcgg ccgcctctcc cgaagcgcgc cacgccttca tcgagctgaa gcccgacgac
     7441 gcgcgcgtgg ctgcgcgcag caccgacgcc gccagggcgg caggtcatcc cctgcccccg
     7501 ctcgccggcc tggccgtcag cgtgaaggac ttgttcgacg tgcagggaca ggttacgcgc
     7561 gccggatcca aggtgttgca tgacgcgccg cccgcgctcg ccgatgcccc ggcggtggcg
     7621 cggttgcgcg aagccggtgc cgcgctgatc ggccgcagca acatgaccga attcgcgttc
     7681 tcgggcgtcg gcatcaatcc gcactacggc acgcccgcca acgtcgcgat gggccgtctg
     7741 gacgctgccg caccgcgcat cccgggcggg tcgacttcgg gcggcgccgt ttcggtggcc
     7801 tgcggcgctg cattcgccgc gctgggctcc gacacgggcg gctccatacg cataccggcg
     7861 gcgctgcagg gcctggtcgg ctacaagaac acggcggcgc tcacgcccac ggcgggcgcc
     7921 ctgcccctgt cgaccacgct ggacaccgct tgcgcgatca cgcgcagcgt gcgtgatgcc
     7981 gtgctgctgc acgaggtgct ggcgcagcgc agcgtgcggc tcgccggccg cccgctcaag
     8041 ggcacgcgct tcgcagtggc cggcacgctg atgcaggacg cactcgaacc ggccgtggcc
     8101 ctcgctttcg agcgcgccct gaaggcgctt gcggcctcag gcgccgcgat cgaagacttg
     8161 ccgctgccca tgctcggcca actggccgcg atgcaatcgc gcggcagctt ctccgcagcc
     8221 gaggcctgga cctggcatcg cagcttgctg gaagcacggg gcgccgacta cgaccaccgc
     8281 gtcgcgcagc gcatatgcac cggcgctgcg atgacggcgg ccgactacat cgaactgcag
     8341 cgtgcccgtc gcgactggat cgcgcgcatg gagaccgccc tgctgggcta tgacgctgtg
     8401 ctgtcgccca cggtgcctat cctggccccg cccatcgctg cgctggaggc cgatgacgcc
     8461 gccttctttc gggccaacgc cgcgctgctg cgcaacacct cggtggccaa catgctcgac
     8521 ggctgcgcca tcagcctacc ctgccaggcc cgcggcgaac cgccggtcgg gctgatgctg
     8581 tgggcggccg gcggccgtga tgatgccctg ctcgatgcgg cgctcgccgt ggaggccgca
     8641 ctcgccgcgg cgctggacgc atgagggtcg cggtggtggg cgccggcatt gccggcgtga
     8701 ccacggctta tgagctcgcc gctgacgggc acgaggtggc cgttttcgag cgccgcggca
     8761 gcgtcgccgc cgaaggcagc tttgccaatg cgggcgtgct cgcctaccac tacgctgcgc
     8821 aatggctctc ggccgagccg cgaagcatcc tgctggggcg cttgttcggc aaccgcacgg
     8881 ccctgagttg gtgtccctcg tggcgtgccg cgcagtggcg caggctttcg cagctcgggc
     8941 gaggcgatgg gcgagctgca cagcagcgtg ccgccgatct gcatgcgctg gcgcgattca
     9001 gcgtcgaacg cctgcagacc ctgacggccc agttgcaact ggagtacgag cagggcgccg
     9061 gctatctggt gctgctgcgc cgcgagcgcg agttggccag gctcaggact gagctggccg
     9121 cgctgcgcga gttgaatctg tcctatcggg agctagaccc cgcccagtgc cgcgcactgg
     9181 agcccggcct caacccggat cagcccctgg ccggtgggat ccgcttcgag caaggccaag
     9241 tggccaattg ccggcagttc gcccatctgc tgcgcgaggc ggcggagcgc cgcggcgccg
     9301 aattccattt cggcgccgcg gccacgctgc tgggtgccga cgccgggggc cccatgctgc
     9361 gcgtggaaca gcatgccctc tcgaccggct tcgcggcgac caccttggcc cacggcaagg
     9421 atgccgtgca ggttcggcgt caggtcatgc agcgccgcag cgcggccgcg gcgcgctatc
     9481 tggaccccgt gagcgagcag cgctacgacg ccgtggtgct gtgcgcgggc gccgcatcgg
     9541 ccaccatgct gcgcactatt ggcctgcgcc tgcctttggt gccggttcac ggctattcga
     9601 tgaccgcgcc gctgcgggcg ccggaacgcg cgccatgctc ggccctgatg gacgagcagt
     9661 acaaggtcgt gatcagccgc atgggccagc gcgtgcgggt agccggcggc gccgaaatcg
     9721 gcggccatcc ggcaaagcac gaccgggcgg ctattgccac gctgtaccgg gtcttgggcg
     9781 actggttccc aggcgccgcc catttggcgc ggccccagct atggaagggt gcccgggcca
     9841 tgctgccagg cggattgccg ctcataggcg cgagcggtgc gcccggcgta tggctcaacc
     9901 tcggccatgg cgccggcggt tggaccctga gctgcggcag tgcccgcctg ctggccgact
     9961 tgatgggcgg ccaccccacc gccgtcgacg cacgcggcta cagcttcgag cgatatgaca
    10021 agcgccaagg ccgctgaagc ggacgaagcg ttgatggcac cgcttgcgcg ccgcggatgc
    10081 ggccggtacc gtgtgcggca tgagccccct ccagcttgtg cgcgtgctgc ccgaacttgc
    10141 gcagcgagtt ccttccctgg gcgcctggcc tttgcacgac gccgccgcga cgcgcgagct
    10201 cgaaacccgg gccgctgcgg ccttgccgag ccacgtgctg atgcagcggg ccgggctcgc
    10261 cgtggccagg ctggcgcagg ctgtcgcgcc ccacgcgcgg cgcgtctgga tcgccgcagg
    10321 ccccggcaac aacggcggag acgggctgga ggcggccatg catctgcatc tggccggccg
    10381 cgaggtgagc gtcagcctgg ctgccgacgg ggcgaggctc cccgccgacg cggcggcgtc
    10441 cctgcagcgt gccaaggccg ccggcgtcag gatcacagac gccatcgagg cgccgcctcc
    10501 gacaccggat gcgctcgcaa tagacgctct gcttggcctg ggcgccagcc ggccgcctgg
    10561 cggcaacatc gcgcaggcga cagacgcgct caaccgtggg gcattacccg tgctggccat
    10621 cgacctgccc tccggcctga atgcgagcac cggccaagcc ttcgaagcct gcgtgcgcgc
    10681 gcgctggaca ctcagcctgc tcacgctcaa gcccggcctc ttcaccagcg cggggcgcga
    10741 ccatgccggt gaagtctggt tcgattcgct cggcgtggcc ggcaatgccg acgcgggctg
    10801 cgcctggtta ccctgtcgcg aaacgctgct cggcctaggc ccgcggcgag cccatagcca
    10861 gcacaagggc agcttcggtg acgtatggat cgtcggcggt gcgccttcga tggttggagc
    10921 ggctttgctg gcggcgcgcg ccagtctgta cgccggcgca ggccgcgtct atctgcatct
    10981 gctcgacgca agcgctgccc gattcgatcc agcccagccc gagctgatgc tgcgcgacac
    11041 gctgccggac gccgccgcct tgcccggctg caccgtcgtc tgcggctgcg gcggcggcga
    11101 ggccgtggcg gcccatctgc ccggcttgct ggtccgcgcc gggcgcctgc tgctcgatgc
    11161 cgatgccctc aacgccgtgg cggccgacgc ggcgctcgcg cagcgcctga gccagcgcgc
    11221 cgcccacggc ctggccacgc tgttgacccc gcatccgctg gaagcggcgc ggctgctcaa
    11281 gcagggcgct gcagacgtgc aagccgaccg cctggcggcc gcacgtgcac tggcacagcg
    11341 ctatcagtcc gtcgtggtgt tgaagggatc aggcagcgtg atctgcgcgc cgggtgcaaa
    11401 acccagcgtc aatcccagcg ggaatgccgc gcttgccagc cccggcaccg gcgatgtgct
    11461 ggcgggctgg ctcggcgggc tgtggagcca gggcttgagc gcccatgacg cggcccggct
    11521 gggcacttat cagcatggcg cggcggcgga tggctggttg gctgaacgcg gcgcggcgct
    11581 cgccttgccg gccggcgcct tggtgcaaag gctcgcgctg ctgccccaac ccacggccac
    11641 ctgacgcagc ccccagccgg cgtgcaggat caacgccgag cccgtttgcc cttgcgctcc
    11701 gcgcgcagga cctgggcacg ctgagctgcc tcgaccttgt cgggttgcac agccgaaacc
    11761 tgcgtcttgg ccgccgcctg cttcttgcct gcgctcgact tcttcgcctt ggcggactgc
    11821 ttgagcgctc ggtctgcatg ctgcacgcct ttgagcgcgt cgaccgccga gccggccttg
    11881 tccgccgcgc cgcgatggga gcgcggcgcg gcgccgcctt cgcgcacgag ccggaagtcg
    11941 atcttgcggc cgtccaggtc gacgcggctg acctgcacac gcacgcgggt tccgactgcg
    12001 tagcgcacgc cggtgcgctc gccgcgcaac tcctggcgcg cctcgtcgaa ccggaaatat
    12061 tcgccgccca gttcggtgat gtggaccagg ccctcgacat agaggttgtc gagctgcacg
    12121 aacaggccga agctggtgac ggcgctgacg gtgccactga actcctcgcc caggtgctcg
    12181 cgcatgaacc tgcacttgag ccaggcctcg acgtcgcgcg aggcctcgtc ggcgcgacgc
    12241 tcgttgacgc tgcagtggat ccccgccgtc tcccattgcg cggcctct
//
LOCUS       DASUCY010000558         3795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1570977, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000558 DASUCY010000000
VERSION     DASUCY010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1570977"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..457)
                     /locus_tag="VFV25_11010"
     CDS             complement(32..457)
                     /locus_tag="VFV25_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006295980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 domain-containing protein"
                     /protein_id="HEY1393899.1"
                     /translation="MTISTTAGYSGTPLAKKLGYKASHRAVVVGAPEGYRDVLEPLPE
                     GVVFERVATAKTDLVHIFATEREVLSRHLNALRKKLKPQAAVWVSWPKKASKVPTDIT
                     EDVIRQVALPLGFVDVKVCAVDDVWSGLKLVVRKGLRAP"
     gene            561..1529
                     /locus_tag="VFV25_11015"
     CDS             561..1529
                     /locus_tag="VFV25_11015"
                     /inference="COORDINATES: protein motif:HMM:NF023354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-dependent oxidative N-demethylase subunit
                     alpha family protein"
                     /protein_id="HEY1393900.1"
                     /translation="MHAASVLARMNFDFDSVTAPFRIQPGLRKLPAGARQLTALSPTS
                     PVFAEKLAVLKNQAAGALLCEAGFDARPALRAVAAQAAAEDPAAFGWEGDSFTAWHLG
                     WRADIETGAVERAGDRALAELDACLEPLPAASRAAALLSLALHEDLAIVDGASVRIPW
                     LAVCLPSRWAPLEKLGRHFAEIHAPVADNALLLAAGEHLMHLVCQPQRWERFVWTITP
                     HAKHDAHPQRHPRGPWPRDADAQALAASAWLRSERQSFIPLPERQQAVFLIHVEIQRL
                     SDAVTTHEQAARLYAALASMSDAVLEYRGLVDARDRLLHWLGGLAS"
     gene            1526..3511
                     /gene="mnmC"
                     /locus_tag="VFV25_11020"
     CDS             1526..3511
                     /gene="mnmC"
                     /locus_tag="VFV25_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HEY1393901.1"
                     /translation="MKTEPIVPARIEFAADNAPRAPDFGDVYHAEAGALEQARHVFLA
                     GNGLPARWGGRQRFVILETGFGLGNNFLATWAAWRAASEAERCGRLVFVSIEKHPATR
                     EDLARAHAVSPLRELARQLIAAWPPATPNLHSLSFEQGRMQLLLAFGDVAALLPEIQA
                     EVDAFYLDGFSPALNPQMWQPRVIKRIGRLAAPSATAATWSAAGAVRGALAEAGFEVE
                     RAPGFAGKRDMTIARYAPRHVPQRAAAALRTPGGPREALVVGAGLAGCAAAWALSRQG
                     WRCRVFEREGEPAAANSGQAGGLMHGTFQGDDGLHARWFRAAALHAERVAKPWVEGGR
                     VAGQLRGALRLEQRLDETSASAQLAASGLPRDYVQWLTREAASELCGVTLPSGAWFYP
                     GGGWLVPRELARAFLAEGEASLEANNDVAALKRAGEDWQLLDAAGRVLAQAPVVVLAN
                     AGTAHALALSACGERPAELWPQQAIRGQTTLLLADTPGLRAPLLPIAGAGYVLPAHRG
                     LVLCGATTQADDREAGLRAEDHRHNLERFGALSGSRPQIDEHQLEKLAGRVGWRITTP
                     DKLPLIGAVPDLAAAQEAARLDQARLVPRLTGAQGGLYVFTALASRGLTSAALGAEVL
                     ASWVSGAPCPVEADLRDALDPARFLVRTWRRAQRPQV"
     gene            complement(3502..>3795)
                     /locus_tag="VFV25_11025"
     CDS             complement(3502..>3795)
                     /locus_tag="VFV25_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1393902.1"
                     /translation="ACAVGLGLALGSVQPMVMSMLHQITPDDRHGEVLGLRMMLLSVS
                     STLMPLLFGGAGTLVGAAPVFWTMALFVGGSSTVARKLEHDLVAAPGHAVHQT"
BASE COUNT          537 a         1359 c         1323 g          576 t
ORIGIN      
        1 tgctgcttcg gccgaagcga agcgccaggg ctcacggggc ccggagcccc ttgcgcacca
       61 cgagcttgag cccggaccag acatcatcga ccgcacacac cttgacatcg acaaagccga
      121 gcggcagcgc gacctggcgt atgacgtctt cggtgatgtc ggtcggcact ttggaagcct
      181 tcttcggcca ggaaacccag acggcggctt gcggcttcag cttcttgcgc agggcgttca
      241 ggtgccggga caacacctcg cgctcggtgg cgaagatgtg gacgagatcg gtcttggcag
      301 tggcaacgcg ctcgaagacg acgccttccg gcagcggctc cagcacgtcc cgatagccct
      361 cgggcgcgcc gaccacgacg gcgcgatgcg aggccttgta cccgagcttc ttcgccagag
      421 gcgtgccgga gtagcccgcc gttgttgaga tagtcatgcg gccccatgcg ctgtaggaag
      481 aaggctcaat gctgccatca gccgccagcc cgcggtcgcc cggcagagcc ctacgccccg
      541 cggctgctca gcagggcatg atgcacgctg catccgtcct tgcgcgcatg aacttcgact
      601 tcgattccgt caccgccccg ttccgcatac agcccgggct gcgcaagctg ccggctggcg
      661 cgcgtcaact cacggccctg agtcccacga gcccggtgtt cgccgagaag cttgccgtgc
      721 tcaagaacca ggccgccggc gccctgcttt gcgaagccgg cttcgatgcc cggcccgcgt
      781 tgcgcgccgt cgccgcccag gccgctgcag aagacccggc ggccttcggc tgggagggag
      841 acagtttcac cgcatggcat ctgggctggc gcgccgatat cgaaacgggc gcggtcgaac
      901 gtgcgggaga tcgcgcccta gccgagctcg atgcctgcct agaacccttg cctgcagctt
      961 cgcgcgccgc cgccttgctg agcctcgcgc tgcacgaaga cctcgccatc gtcgatggcg
     1021 cctcggttcg catcccttgg ctggccgtct gcctgccgtc gcgctgggcg ccgctcgaaa
     1081 agctcggccg ccatttcgcc gagatccatg caccggtggc cgacaacgcg ctgctgctgg
     1141 ccgcgggcga gcatctgatg catctggtct gccagcccca acgctgggag cgtttcgtct
     1201 ggacgatcac gccgcacgca aagcacgacg cgcacccgca gcgccatccg cgcgggccct
     1261 ggccgcgcga cgccgatgcg caggcgctgg ccgcgtcggc ctggctgcgc agcgagcgcc
     1321 agagtttcat ccccctgccc gagcgacagc aggcggtctt cctgatccat gtcgagatcc
     1381 agcgcctgag tgatgcggtg acgacgcatg aacaggcggc gcgcctgtac gcggcgctgg
     1441 cgagtatgag cgacgctgtg ctggagtacc gtggcctcgt cgatgcccgc gatcgcttgc
     1501 tgcattggct cgggggcctt gcgtcgtgaa gaccgagccc atcgtacctg cccgcatcga
     1561 gttcgctgcc gacaatgcgc cgcgcgcgcc cgacttcggc gacgtctatc acgccgaggc
     1621 cggcgcgctg gagcaggcgc gacacgtctt cctggcgggc aacgggctgc ccgcgcgctg
     1681 gggcgggcgc cagcgcttcg tgatcctgga aaccggcttc ggcctgggca acaacttcct
     1741 cgccacctgg gccgcctggc gcgcggcaag cgaggccgag cgctgcgggc ggctggtctt
     1801 cgtctccatc gagaagcatc cggccacacg cgaagatctg gcccgggcgc atgccgtctc
     1861 gccgctgcgc gagctcgcgc ggcagttgat cgccgcgtgg ccgccggcaa cgccgaatct
     1921 ccattcgctc tccttcgagc aaggccgcat gcaattgctg ttggccttcg gcgacgtggc
     1981 cgcgctgctg cccgagatcc aagccgaggt cgacgccttc tatctcgacg gcttctcgcc
     2041 cgccctcaat ccgcagatgt ggcagccgcg cgtgatcaag cgcatcggcc ggctggccgc
     2101 gccgagtgcc accgcggcga cctggagcgc ggcaggcgcg gtgcgcggtg ccctggcgga
     2161 ggccggcttc gaggtcgagc gcgcaccggg ctttgccggt aagcgcgaca tgacgatcgc
     2221 gcggtatgcg ccgcgccatg tgccccagcg cgctgccgcg gccctgcgca cgcccggcgg
     2281 cccgcgcgag gcgctagtcg tcggcgccgg cctggccggc tgcgcggcgg cctgggcctt
     2341 gagccggcag ggctggcgct gccgcgtctt cgagcgcgaa ggcgaaccgg cagcggccaa
     2401 ttccggccag gcaggcgggc tgatgcacgg caccttccag ggtgacgatg gcctgcacgc
     2461 gcgctggttc cgcgccgccg ccttgcacgc cgagcgcgtc gcgaagccct gggtcgaggg
     2521 cggccgcgtt gccgggcaat tgcgtggcgc tttgaggctg gagcagcgtc tcgatgaaac
     2581 gagcgcaagc gcgcagttgg ccgcgtcagg gctgccgcgc gactatgtgc aatggctcac
     2641 gcgcgaggcg gcgagcgagc tgtgcggcgt caccttgccg agtggtgcat ggttctaccc
     2701 gggcggcggc tggctggtgc cgcgcgagct ggcgcgcgcc tttctggcgg aaggcgaggc
     2761 cagcctggag gcgaacaatg acgtcgctgc cttgaaacgc gccggcgagg actggcaatt
     2821 gctcgacgct gccggccgcg tgctggccca ggctcctgtc gtggtactgg caaatgccgg
     2881 caccgcccat gcgctggcgc tcagcgcgtg cggtgagcgc ccagccgagt tgtggccgca
     2941 gcaggccatc cgcggccaga ccacgctgct gctggccgat acgccgggcc tgcgcgctcc
     3001 tctgttgccc atcgccggcg cgggctatgt gctgcccgcg catcgcggcc tggtgctatg
     3061 cggtgcgacc acgcaggccg atgatcgcga ggccggtttg cgagcggagg atcatcggca
     3121 taacctggag cgcttcggcg ctctcagcgg cagccggccg cagatcgacg agcaccagtt
     3181 ggagaaactg gccggccgcg tcggctggcg catcactacg cccgacaagc tgccgctcat
     3241 cggcgccgtg cccgacctgg ctgcagccca ggaggcggcc cgcctcgatc aagcccgcct
     3301 cgtgccgcgg ctcacgggcg cacagggcgg gctctacgtg ttcacggccc tggcctcgcg
     3361 cggcctgacc tcggcggcgc tgggcgccga agtgttggcc tcctgggtca gcggcgcgcc
     3421 ctgccccgtg gaagccgatc tgcgcgatgc gctggaccca gcgcgcttcc tggtacgaac
     3481 ttggcggagg gcacagcggc ctcaggtctg atgaacggcg tgccctggtg ccgccaccag
     3541 atcatgctcg agcttgcgcg ccacggtgct gctgccgccg acgaagagcg ccatggtcca
     3601 gaacaccggc gccgcgccca ccagcgtgcc ggcgccgcca aagagcagcg gcatcagcgt
     3661 gctggagacg ctgagcagca tcatgcgcag gcccagcacc tcgccgtggc gatcgtccgg
     3721 cgtgatctgg tgcagcatgg acatcaccat cggctgcacc gagcccagcg ccaggcccag
     3781 gcccaccgcg caggc
//
LOCUS       DASUCY010000559         3875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_13137, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000559 DASUCY010000000
VERSION     DASUCY010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13137"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="VFV25_11030"
     CDS             complement(<1..893)
                     /locus_tag="VFV25_11030"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEY1393903.1"
                     /translation="MTPIPTPGLRRPFPLALALARALLLCGLALTGGIAQAAAPRTAD
                     YIVAVVNRELVTNAEVEQRMERIEQDAKRSGARLPPHDELRKQIVDGLIEERAQLSYA
                     KDVGIKVDESELDRAVASVAANNQVTMDELRARLRKDNVSYERFRSNLRDQMMLERVR
                     EREVQSKVKVTDAEIDAWLAKQRSAGTAPAQYNIAQILIATPDGATMVQTAVLLARAQ
                     EALKRAQAGESFDALVREYSNGPKDHGGEMGLRPADRLPDLFVEAVRNLKPGEMAQQV
                     LRSGAGFHVLKLVDKSEADAM"
     gene            complement(916..3288)
                     /gene="lptD"
                     /locus_tag="VFV25_11035"
     CDS             complement(916..3288)
                     /gene="lptD"
                     /locus_tag="VFV25_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HEY1393904.1"
                     /translation="MLALAAWSLAASAQSSDVPPAPAAASAPAAAASVPDRTKLPISY
                     QADSVQGRPDQETVLEGHVKLRQDGMSLGADRVEYNAPDDLARATGRVRIVRDGNVYA
                     GSELSIKLPQFEGYFLKPSYYFGLTQTGGRADRIDFADPRHITASGATYTSCPASDDG
                     TPDWVLSTGSIKLDFDTGEGVAEHAVLRFLGVPILALPSMSFPLTDERKSGWLPPDIN
                     LSNTNGFELGVPYYWNIAPNYDATLTPLLMTRRGFGVDGEFRYLEPNWRGTLVVDTLP
                     ADQVARRNRWALRSIQLGELPNNAYYSWRVQRTSDDGYWKDFPGKLESITPRMLASNG
                     QLQRDWLGEDLVTSAYARVEAWQVLQDPSNPIVPPYQREPQIGVRQRGAKAGWQWSWE
                     TEINRFTLEDSTLTGSSAPNGLPNGTRVHALGALSRPLAMGGWTLTPKVSLNAASYDL
                     SQPMANGQTRASRLIPSFSLDSAWVFERATTLFGKDTTQTLEPRLFYVNTPYRAQSFL
                     PTFDSAAKDLNFTSVYTENAFSGVDRVSDANQITAGVATRYLDTATGAEVLRLGAAQR
                     YLLRDQLITPDDGPPVTTRLSDLLLLASTTLVPKWKLDSSLQYSYDQHKLERSTTSVR
                     YSPGPFRTVSATYRLTRGLTEQVELGWQWPISGLTPLEKPVTKPEPDAMPASMLGAAF
                     SGSSGKCKGSWYTVGRISYSLLDRRITDSIVGLEYDAGCWIGRIVAERLSTGTSESTT
                     RLMLQLELVGFSRLGSNPLKTLKDNIPGYRLLRDDALPGTPSGTSTATYE"
     gene            3411..>3875
                     /locus_tag="VFV25_11040"
     CDS             3411..>3875
                     /locus_tag="VFV25_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEY1393905.1"
                     /translation="MGIRLGNVSHNPEQTQATISWQDEARRAAFEQWLATLTPARGLD
                     AASLRPASADASFRRYFRTDAAAGPLVIMDAPPSQIACRPFVQMAELLRGAGLNAPEV
                     LDWDEALGFMLLSDLGETTYLSHLGRLDPAQPQAARELYLDALAALVKLQGIA"
BASE COUNT          545 a         1252 c         1420 g          658 t
ORIGIN      
        1 ctcatcgcat cggcctcgct cttgtcgacc agcttgagga cgtggaagcc cgcgcccgaa
       61 cgcagcacct gctgcgccat ctcgcctggc ttgagattgc gcaccgcttc aacgaagaga
      121 tcgggcagcc tgtcggccgg ccgcagcccc atttcgccgc catggtcctt ggggccgttg
      181 gaatattcgc gaaccagggc gtcgaaggac tcgccggcct gggcgcgctt gagcgcctcc
      241 tgggcgcgcg ccagcagcac tgcggtttgc accatggtcg cgccgtccgg tgtggcgatc
      301 aggatctgcg cgatgttgta ctgggcgggt gccgtgccgg cgctgcgctg cttggccagc
      361 caggcgtcga tctcggcgtc ggtcaccttg accttggact gcacctcgcg ctcgcgcacc
      421 cgctcgagca tcatctggtc gcgcagattg ctgcggaagc gctcgtagct cacgttgtcc
      481 ttgcgcaggc gcgcgcgcag ctcgtccatc gtgacctggt tgttggcggc cacgctggcg
      541 acggcgcggt ccagctcgga ctcgtcgacc ttgatgccga cgtccttggc atagctgagc
      601 tgggcgcgct cctcgatcag gccgtcgacg atctgcttgc gcagctcgtc atgcggcggc
      661 aggcgggcgc cgctgcgctt ggcgtcctgc tcgatgcgct ccatgcgctg ctcgacctcg
      721 gcattggtga ccagctcgcg gttgacgacg gcgacgatgt agtcggcggt gcgcggcgcg
      781 gcggcctgcg cgatgccgcc cgtcagtgcc aggccgcaca gcagcagcgc gcgcgccagc
      841 gccaatgcca atgggaaagg ccggcgcagg ccgggcgtag ggatgggcgt cacggcttgg
      901 tgatggtgct gtcgttcatt cataggtggc agtggacgtg ccggagggcg tgccgggcag
      961 cgcatcgtcg cgcagcaggc ggtagccggg gatattgtcc ttcaaggtct tgagcgggtt
     1021 gctgcccagg cgcgagaagc ccaccagttc gagctgcagc atcaggcgcg tggtcgattc
     1081 cgaggtgccg gtggacagcc gctcggcaac gatgcgcccg atccagcagc cggcgtcgta
     1141 ttcgaggccg acgatggaat cggtgatgcg cctgtcgagc aggctgtagc tgatgcggcc
     1201 cacggtgtac cagctgccct tgcacttgcc gctgctgccc gagaacgccg cgcccagcat
     1261 cgaggccggc atggcgtccg gctcaggctt cgtgacgggc ttctccaaag gggtgaggcc
     1321 tgagatcggc cattgccagc cgagttccac ctgctcggtc aggccgcgcg tcaggcggta
     1381 ggtggcgctg acggtgcgga aagggccagg cgaatagcgc acgctggtcg tggagcgctc
     1441 cagcttgtgc tggtcgtagc tgtactgcag cgagctgtcg agcttccatt tcggcaccag
     1501 cgtcgtcgag gccagcagca gcaggtccga caggcgcgtg gtcacgggcg gcccgtcgtc
     1561 gggcgtgatg agctggtcgc gcagcaggta gcgctgcgcg gcgcccaggc gcagcacctc
     1621 ggcgccagtg gccgtgtcga ggtagcgcgt ggccacgccg gcagtgatct ggttggcgtc
     1681 cgagacacgg tccacgcccg agaaggcgtt ctcggtgtag acgctggtga agttgaggtc
     1741 cttcgcggcg ctgtcgaaag tcggcaggaa ggattgggcg cggtagggcg tgttgacgta
     1801 gaacagccgc ggctccaggg tctgggtcgt gtccttgccg aacagcgtcg tcgcgcgctc
     1861 gaacacccag gcactgtcca ggctgaagct cgggatcagg cgcgaagccc gcgtctggcc
     1921 gttggccatg ggctgcgaga ggtcgtagga ggcggcgttg agggagacct tgggcgtgag
     1981 cgtccaaccg cccatggcca gcgggcggct gagcgcgccc agggcatgca cgcgtgtgcc
     2041 gttcggcagt ccgttgggcg cgctgctgcc ggtcagcgtg gagtcttcga gcgtgaagcg
     2101 gttgatctcg gtctcccagc tccattgcca gccggctttc gcgccgcgct ggcgcacgcc
     2161 gatctgcggc tcgcgctggt agggcggaac gatgggattg ctcgggtcct gcagcacctg
     2221 ccaggcctcg acgcgcgcat aggcgcttgt cacgagatcc tcgcccagcc agtcgcgctg
     2281 cagctggccg ttggaggcga gcatgcgcgg cgtgatgctt tccagcttgc cggggaagtc
     2341 cttccagtag ccgtcgtccg aggtgcgctg cacgcgccag gagtagtagg cgttgttggg
     2401 cagctcgccc agctggatcg agcgcagggc ccagcggttg cgcctggcga cttggtcggc
     2461 gggcagggtg tcgacgacca gcgtgccgcg ccagttgggc tcgaggtagc ggaactcgcc
     2521 gtccacgccg aagccccggc gcgtcatcaa caggggcgtg agggtcgcgt cgtagttggg
     2581 cgcgatgttc cagtaatagg gcaccccaag ctcgaagcca ttggtgttgc tcaggttgat
     2641 gtcgggcggc agccaaccgg acttgcgctc gtcggtgagc gggaagctca tcgacggcag
     2701 ggccaggatg ggcacgccca ggaagcgcag cacggcatgc tcggcgacgc cctctcccgt
     2761 gtcgaagtcc agcttgatgc tgccggtgct cagcacccag tcgggcgtgc cgtcgtcgct
     2821 ggcggggcag ctcgtgtagg tggcgccgct ggccgtgatg tgacggggat cggcgaagtc
     2881 gatgcgatcg gcccgcccgc cggtctgggt gagcccgaag taatagctgg gcttgaggaa
     2941 atagccttcg aactgcggca gcttgatgct gagctcgctg ccggcataga cattgccgtc
     3001 gcgcacgatg cgcacgcggc cggtggcacg cgccaggtcg tccggcgcgt tgtactcgac
     3061 gcggtccgcg cccagcgaca tgccgtcctg gcgcagcttc acatggcctt cgagcacggt
     3121 ttcctggtcg gggcggccct gcacgctgtc ggcctggtag gagatgggca gcttggtgcg
     3181 atccgggacg ctggcagccg ctgcgggcgc gctggccgcc gccggcgcgg gggggacgtc
     3241 actgctctgc gcggatgctg ccaggctcca ggcggccagc gccagcacgc aggccaaccg
     3301 gctgcatgca ggcacaagag cgggcgggca acgcttcagc gggacgggca aggcaggcaa
     3361 ggcggcgctg aggcgcgcga gggtgatttg tagaatcgat cgattatttc atgggcatcc
     3421 gcctcggcaa cgtgagtcac aaccctgagc agacgcaggc gaccatctcc tggcaggacg
     3481 aagcccgtcg tgcggccttc gagcagtggc tggccaccct gacaccggcg cggggcctcg
     3541 atgccgcgag cctgcgcccg gccagcgccg atgcgagctt tcgccgctac ttccgcaccg
     3601 acgccgcggc cggcccgctc gtcatcatgg acgccccgcc gtcccagatc gcctgccgcc
     3661 ccttcgtgca gatggccgag ctgctgcgcg gcgccggcct caatgcgccc gaggtgctgg
     3721 actgggacga ggcgctgggc ttcatgctgt tgtccgacct cggcgagacg acctatctgt
     3781 cgcacctggg gcgcctggac ccggcccagc ctcaggccgc gcgcgagctc tatctcgacg
     3841 cgctcgcggc cctggtcaag ctgcagggca tcgcg
//
LOCUS       DASUCY010000560         4193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1570998, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000560 DASUCY010000000
VERSION     DASUCY010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4193
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1570998"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /locus_tag="VFV25_11045"
     CDS             <1..968
                     /locus_tag="VFV25_11045"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEY1393906.1"
                     /translation="LDRNSVTLIPQAPGIARNAKGEPLASDELVVAANLPLRSGGPDE
                     EGSVQLRGIGEQAWAVRPQVKVIEGRRFEPGLRELIVGRGAARQFAGMVPGQEIRLGT
                     QQWKVAGIFASGDAMESEIWADAPVVADTYRRGSSRGSVTVKLTDAQAFSAFKTSLEA
                     NPQLKVDVTTTLDYFSKQSEGVTKVLRVIGITVGAIMAIGAMFGALNTMFAAVAARAR
                     EIATLRAIGFAGLPVVVAVMLETMLLALIGGVIGGLLAWLLFNGDNASTLAAGTVGQL
                     AFQLHVTPELLWTGLKWALAIGFIGGLFPAMRAARLPVTTALREA"
     gene            complement(1021..2157)
                     /gene="mnmA"
                     /locus_tag="VFV25_11050"
     CDS             complement(1021..2157)
                     /gene="mnmA"
                     /locus_tag="VFV25_11050"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEY1393907.1"
                     /translation="MSKTRVVVGLSGGVDSAVSAHLLKQQGYEVVGIFMKNWEDDDDD
                     EYCSSRQDFLDAASVADLLGIEIEHVNFAAEYKDRVFAEFLREYQAGRTPNPDVLCNA
                     EIKFKAFLDHAMRLGAEKIATGHYARVRYNEATGLHELLKGLDHSKDQSYFLHRLNQA
                     QLAKTLFPVGELKKTEVRRIAAEIGLPVAAKKDSTGICFIGERPFREFLNRYLANQPG
                     PIKDERGRVIGEHVGLSFYTLGQRKGIGIGGLKDKGGKPGATPGQASRGGGDHDPWYV
                     ARKDLAANTLWIVQGHDHPWLQSHELSADEASWVAGAAPSPGAPAAKTRYRQADAACR
                     LEAGANGEFALAFSEAQWAVTPGQSAVLYDGEVCLGGGFIAGSR"
     gene            2369..2722
                     /locus_tag="VFV25_11055"
     CDS             2369..2722
                     /locus_tag="VFV25_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393908.1"
                     /translation="MSSNVVFFAWNRSIPGRERLSAEHFGEFLQYLGELQKKGTIQAF
                     DPVFLDPHGGDLNGFILLRGDSAKLDGLLASKEWVTHVTRGLLHLEGSGAVRGVTGDE
                     LMERMKLWTGLLPAP"
     gene            2779..3249
                     /locus_tag="VFV25_11060"
     CDS             2779..3249
                     /locus_tag="VFV25_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018425186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEY1393909.1"
                     /translation="MAMLDKQEQVVAVLNRLLEQELAGVVRYTHYSFLVFGFGRIPIV
                     AWLREQADESLRHAQALGEWITTLGAYPSLAIGPLLDSHQAEVGAMLRESLEAEGQAL
                     RLYRELLGLVEGRSVALEEFARQMIHTEELHAAEVDKMLRKPGQLTPFSALSDK"
     gene            complement(3267..3767)
                     /locus_tag="VFV25_11065"
     CDS             complement(3267..3767)
                     /locus_tag="VFV25_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEY1393910.1"
                     /translation="MSKSRWKPSVTVAAVIERNGRFLLVEEHTPEGLMLNNPAGHLDP
                     GESPEQGVIREAREETGRVFEPEALLGVYLSRFRRPAAGEDVTYVRFAFCGTAGEPAP
                     DWVLEPGIVRALWLTPEEIRASAMRHRSPLVLRCVEDHLAGRRYPLELLNTDPTVFDP
                     QIKRVG"
     gene            3918..>4193
                     /locus_tag="VFV25_11070"
     CDS             3918..>4193
                     /locus_tag="VFV25_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HEY1393911.1"
                     /translation="MLIGVPRETAAGETRVAVTPETAKKLKAQGHTLRIQSGAGVAAS
                     APDAAYEAVGAEICDEAGAFGAELVLKVRTPNAEERALMKPGTALVGM"
BASE COUNT          656 a         1375 c         1439 g          723 t
ORIGIN      
        1 tgctggaccg caacagcgtc accctcattc cccaggcgcc cggcatcgcg cgcaatgcca
       61 agggcgagcc gctcgcgtcg gacgagctgg tggtcgcagc caacctgccc ctgaggagcg
      121 gcggccccga cgaagagggc agcgtgcaac tgcgcggcat cggcgagcag gcctgggctg
      181 tgaggccgca ggtcaaggtc atcgagggcc ggcgcttcga gcccggattg cgcgagctga
      241 tcgtgggccg cggtgcggcg cgtcagttcg ccggcatggt gccgggccag gagatccgcc
      301 tgggcacaca gcagtggaag gtggccggca tcttcgcgtc cggcgacgcg atggagtcgg
      361 agatctgggc cgacgctccc gtggtcgcag acacctaccg ccgcggcagc agccgcggct
      421 cggtgactgt caagctcacc gacgcgcagg ccttcagcgc cttcaagacc tcgctggaag
      481 ccaaccccca gctcaaggtc gacgtgacca ccacgctgga ttacttcagc aagcagtccg
      541 aaggcgtgac caaggtgctg cgtgtcatcg gcatcaccgt gggcgcgatc atggcgatcg
      601 gcgcgatgtt cggcgcgctg aacacgatgt tcgccgcggt ggcggcgcgc gcgcgcgaga
      661 ttgccacgct gcgtgcgatc ggcttcgcgg gcctgccggt ggtggtggcg gtgatgctgg
      721 agaccatgct gcttgccctg atcggcggcg tcatcggagg cctcctggcg tggctgctct
      781 tcaacggcga caacgcctcg acgctggcgg ccggcacggt cggccagctc gctttccagc
      841 tgcatgtcac gcccgagctg ctgtggaccg gcctgaaatg ggcgctggcg atcggcttca
      901 tcggcgggct cttccccgcg atgcgcgccg ccaggctgcc ggtgacaaca gcgctgcgcg
      961 aggcctgagc ggccttgccg caaacgatgg gcggccgtgc gctagcgcgg cctgccccag
     1021 ctagcggctg ccggcgataa aaccaccgcc caggcagacc tcgccgtcgt agagcacggc
     1081 cgactgcccc ggcgtcaccg cccactgcgc ctcggagaaa gccagcgcga attcgccgtt
     1141 ggcccccgcc tcgagccggc atgcggcatc ggcctggcgg tagcgcgtct tggctgccgg
     1201 tgcaccgggc gaaggtgccg cgccggccac ccagctggct tcgtcggcgc tcagctcatg
     1261 gctttgcagc caggggtggt cgtgaccctg cacgatccac agcgtgttgg ccgccagatc
     1321 cttgcgcgcg acgtaccagg gatcgtggtc gccgccgccg cgcgatgcct ggccgggcgt
     1381 tgcgccgggt tttccgcctt tgtccttcag cccgccgatg cctatgccct tgcgctggcc
     1441 cagcgtgtag aagctcaggc ccacgtgctc gccgatcacg cggccgcgct cgtccttgat
     1501 tggccccggc tggttggcga gatagcggtt gaggaattcg cgaaacggcc gctcgccgat
     1561 gaagcagatg ccggtggaat ctttcttcgc cgccaccggc aggccgatct cggccgcgat
     1621 gcggcgcacc tcggtcttct tgagttcgcc gaccgggaac agcgtcttgg ccagctgcgc
     1681 ctggttgagg cggtgcagga aatagctttg gtccttgctg tggtccaggc ccttgagcag
     1741 ttcgtggagc cctgtggcct cgttgtagcg cacgcgggca tagtgacctg tagcaatctt
     1801 ctccgcgccc aggcgcatcg catggtcgag gaaggccttg aacttgatct cggcgttgca
     1861 caacacgtcg gggttgggcg tgcgcccggc ctggtactcg cgcaggaact cggcaaacac
     1921 gcggtccttg tactcggccg cgaagttcac atgctcgatc tcgatgccca gcaggtcggc
     1981 caccgaggct gcgtccagaa agtcctgacg cgacgagcag tattcgtcgt catcgtcatc
     2041 ttcccagttc ttcatgaaga tgccgacgac ttcatagcct tgctgtttga gcaggtgcgc
     2101 gctgaccgcg gaatcgacac caccgctcaa gcccaccact actcgcgtct tgctcatgat
     2161 gcaaatttta tggggcgccg cttcagcggg ccttgtgccg cctgcaagcc cttgcgcctt
     2221 agctgccgat cggccttgag tgttgaaagg cgagcactaa actgatttgg tcggccggct
     2281 gagcccagcg gtcggctctg gctctcgagt tccgcggctc ggcggggacg ccacgggacc
     2341 ccatcggcag ccccgctgag gagacgcgat gagttccaac gtcgttttct tcgcctggaa
     2401 ccgctccatt ccggggcgcg agcgcctgag cgcagagcat ttcggcgagt tcctgcagta
     2461 cctgggcgag ctgcagaaga aaggcacgat ccaggccttt gatcccgttt tcctcgatcc
     2521 gcatggcggc gacttgaacg gcttcatcct tctgcgaggc gacagcgcca agctggatgg
     2581 cctgctggcc agcaaggaat gggtcaccca tgtcacgcgc gggctgctgc acctcgaagg
     2641 ctcgggcgcg gtgcgcggcg tcaccggtga cgagttgatg gagcgcatga agctgtggac
     2701 cgggctgttg ccggccccat agcgttgacg gtcccgaccc ggcggcgccg ggtcggcaca
     2761 gatttggagg ctgctgccat ggcgatgctc gacaagcagg agcaagtggt ggccgtgctg
     2821 aaccgcctgc tcgaacagga gctggctggc gtggtgcgct acacgcatta ctccttcctc
     2881 gtgttcggct tcggccgcat ccccatcgtg gcctggctgc gcgagcaggc cgacgaatcg
     2941 ctgcggcacg cgcaagcgct gggcgaatgg atcacgacct tgggtgccta tccgtcgctc
     3001 gcgatcgggc cgctgctcga ttcgcatcag gccgaggtgg gtgcgatgtt gcgcgaatcg
     3061 ctggaagccg agggccaggc gctcaggctc taccgcgaac tcctcggcct ggtggagggc
     3121 cgctcggtgg cgctggaaga gtttgcgcgt cagatgatcc acaccgagga attgcatgcc
     3181 gccgaggtcg acaagatgct gcgcaagccc ggccagctga cgccgttttc agcgctgtcg
     3241 gacaagtagg gcggctcgta gatccgtcat cccacacgct tgatctgcgg atcgaacacg
     3301 gtcgggtcgg tgttcagcag ctcgagcggg taacgcctgc ccgccaggtg gtcctcgacg
     3361 cagcgtagta ccagggggct gcggtggcgc atggccgagg cgcggatctc ctcgggcgtc
     3421 agccacagcg cgcggacgat gcccggctcc agcacccagt ccggcgccgg ctcgcccgcg
     3481 gtgccgcaga aggcaaagcg cacataggtc acgtcctcgc cggcagcggg acgccgaaag
     3541 cgcgacagat agacgcccag cagggcctcg ggctcgaaga cgcggccggt ttcctcccgc
     3601 gcctcgcgta tcacgccttg ctcgggcgac tcgccggggt ctagatggcc ggccgggttg
     3661 ttgagcatca ggccctccgg cgtgtgctcc tcgaccagca ggaagcggcc gttgcgctcg
     3721 atcaccgcag ccaccgtcac gctgggcttc catcggcttt ttgacatggg ggcaatgata
     3781 ggcaggtgga ttcccatcag gcagggtgca agtgtccgcc acaatccagg tttggccggg
     3841 tttgtcagtt tgtgtaagct gcgcccaggc ttaccttgca aaccaaccaa cgatccgagt
     3901 caagctagga gggttagatg ctcatcggag tcccgcgaga gacggcggcg ggggagacgc
     3961 gcgtagccgt cacgcccgag acggccaaga agctcaaggc ccagggccat accctgcgca
     4021 tccagtcggg cgccggcgtg gcggccagtg cgccggacgc ggcctatgag gccgtgggcg
     4081 ccgagatctg tgacgaagcc ggggccttcg gggccgaact ggtgctcaag gtgcgcacgc
     4141 ccaatgccga agagcgcgca ttgatgaagc ccggcacggc cctggtcggc atg
//
LOCUS       DASUCY010000561         2248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1426934, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000561 DASUCY010000000
VERSION     DASUCY010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1426934"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="VFV25_11075"
     CDS             complement(<1..665)
                     /locus_tag="VFV25_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY1393912.1"
                     /translation="MKLKLKIPLYFVGALLLSLAAGVGGLLVVSHSLNTFQQDVMQQV
                     DDERAIASLASHFKTQVQEWKDTLLRGSDSALLDKHWTAFGAQQKAVADGAQALRGKL
                     GEDSLNTQLDQFIAAHQKMAAGYADGFEKFKNTGFDSSVGDMAVRGIDREPAKLLEGI
                     GRQIAERRVKVAQAAFADGHHAFWLAIAGMFAACGIGLGVGVLLGRRVVAPLVEATHL
                     ARDV"
     gene            complement(823..2103)
                     /locus_tag="VFV25_11080"
     CDS             complement(823..2103)
                     /locus_tag="VFV25_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393913.1"
                     /translation="MSHRTALAAGLAALSFTAAATAADQTAPGAGNPYADRIAAASPR
                     VREAHVFLVEQAMKIRNVALRAATLDLLRNRGFCITSRAGVNDAVKSSMLAELGAAGL
                     INAGDDASFPGGLKAGVFPAMLDEASACPHMPMSFDAAPGSSFGSHHGYPGGLPIHEA
                     NNLRAALGLAEGYRTSYRAAPGQALQDEHGDEDEASGWMKSPFFIDQDIVIAAPIWHD
                     WAKTLVFQWTAEGQEFKELNFGGTPANGSATGGHHIISVGESMKRGLPAAFVIAQASA
                     HSNPTLGNEFKVVNWLRAAGILAQVDPVKAGYLMQDAKGGFHLPALRKLAEGFDLAGA
                     GQTNLLPEYTIHNLSDGDFTFSIPAAGDAALLVAKLAPQFGFDPADTARYNNAYRNPV
                     FANLSQERILILYANGGLAAVQAELEALRARRVF"
BASE COUNT          358 a          747 c          778 g          365 t
ORIGIN      
        1 acatccctcg cgagatgcgt cgcctcgacc agcggcgcga cgacgcgccg gccgagcagg
       61 accccgaccc cgaggccgat gccgcatgcg gcgaacatgc ccgcaatggc gagccagaag
      121 gcgtgatggc catcggcgaa agcggcctgc gcgaccttga cgcggcgctc ggcgatctgg
      181 cggccgatgc cctccaggag cttcgccggc tcgcgatcga tgccgcgtac ggccatgtcc
      241 cccacggacg aatcgaagcc cgtgttcttg aacttctcga agccgtcggc atagccggcg
      301 gccatcttct ggtgcgccgc gatgaactgg tcgagctggg tgttcaggct gtcttcaccc
      361 agcttgccgc gcagcgcctg cgcgccgtcg gcgaccgcct tctgctgcgc gccgaacgcg
      421 gtccagtgct tgtcgagcaa ggcgctgtcc gagccccgaa gcagcgtgtc cttccactcc
      481 tgcacctgcg tcttgaagtg gctagccagg ctggcaatgg cgcgctcgtc atcgacctgc
      541 tgcatcacgt cctgctggaa ggtgttgagc gaatggctga cgaccagcag gccgccgaca
      601 cccgcggcca gcgacagcag caacgcgccg acaaagtaga ggggaatctt gagcttgagt
      661 ttcacgacca ctgtccttgg gcggacacga aggccgcact actcaagcgc tttatcggcc
      721 ggccgccggg ttccttgagg cattcaaatc gatgcagcct cgccctgcgc agcgctgatc
      781 ggtgcgcagg gctgcatcgg ccgtgcttgc ccgctacaac aatcagaaca cgcggcgagc
      841 ccgcagcgct tcgagctctg cctgaaccgc ggccaggccg ccgtttgcgt acaggatcag
      901 gatccgctcc tggctgaggt tggcaaacac cggattgcgg taggcgttgt tgtagcgcgc
      961 ggtgtcggcc gggtcgaagc cgaactgcgg cgccagcttg gcgaccagca atgcagcatc
     1021 gcccgcggcc gggatcgaga acgtgaaatc gccgtccgac aggttgtgga tggtgtattc
     1081 cggcagcagg ttggtctgcc cggcgcccgc cagatcgaag ccctcggcca gcttgcgcag
     1141 cgcggggagg tggaagccgc ccttcgcatc ttgcatcaga tagccggcct tgaccggatc
     1201 gacctgggcc aggatgccgg ccgcgcgcag ccagttcacc accttgaact cgttgcccag
     1261 cgtcgggttc gagtgggccg aggcctgggc gatcacgaag gccgccggca ggccgcgctt
     1321 catcgattcg cccacgctga tgatgtgatg gccgccggtg gccgagccgt tcgcaggcgt
     1381 gccgccgaag ttcagctcct tgaactcctg gccctcggcg gtccactgga acaccagggt
     1441 cttggcccag tcgtgccaga tcggggccgc gatgacgatg tcctggtcga tgaagaaggg
     1501 cgacttcatc cagccgctgg cttcgtcctc gtcgccatgc tcgtcctgca gcgcttggcc
     1561 cggtgccgcg cgatagctcg tccgatagcc ctcagccagg ccgagcgcgg cgcgcagatt
     1621 gttggcctcg tggatcggca gcccgcccgg atagccgtgg tggctgccga agctgctgcc
     1681 cggcgcggcg tcgaaggaca tcggcatatg cgggcaggcg ctggcttcgt ccagcatggc
     1741 cgggaagacg ccggccttca gcccgcccgg gaagctggcg tcgtcaccgg cgttgatcaa
     1801 cccggccgcg ccgagctcgg ccagcatgct gctcttgacg gcgtcgttca cgccggctcg
     1861 ggaagtgatg caaaagccgc ggttgcgcag caggtcgaga gtcgcggcac gcagagccac
     1921 attgcggatc ttcatggcct gctcgaccag gaagacgtgg gcctcgcgca cgcgcggcga
     1981 ggcggcggcg atgcggtcgg catacgggtt gccggcgccg ggcgccgtct ggtcggcggc
     2041 agtcgccgcg gccgtgaacg aaagcgctgc gagacctgca gccagggcgg tgcggtgaga
     2101 cacggtgggt tctccttgtt gaatggcccc agggctcggc aaagcgagcc cgccccgagg
     2161 gggtcaagga aacggggcgg cccatcgttt ccttgagaga cggccgcagt gtcagtcgca
     2221 cagatgacag ctcggtggca aggccggg
//
LOCUS       DASUCY010000562         2120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1474945, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000562 DASUCY010000000
VERSION     DASUCY010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1474945"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2120)
                     /locus_tag="VFV25_11085"
     CDS             complement(<1..>2120)
                     /locus_tag="VFV25_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEY1393914.1"
                     /translation="EPGTSPDLALVDVQNRLSRASPRLPSAVTQQGVRVDKARSNFLL
                     FAILSSDNPAYDPIALGDYASRNLLPEIQRVPGVGQAQLFGTERAMRIWIDPAKLTGF
                     RLSAAEVNNAIRGQNAQVSAGGIGNLPLQPGQAISATVTVGGQLSTIEQFGNIVLRAN
                     ADGSSVRLRDVARIELGAQTYATQARLDGRPSTGIGVQLSPTGNALATATAVRQKVED
                     LSRYFPPGVKATIPYDSSRFVRISIRQVVETLFEAVVLVFLVMYLFLQDIRYTLIPTM
                     VVPVTLMGTFGVLLALGFSINVLTMFGMVLVIGIVVDDAIVVVENVERIMSEEGLPPR
                     AATRKAMGQIQGAIVGVTVVLASVFVPLAFFGGSVGNIYRQFSAVMVAAILLSAFMAL
                     SLTPALCATLLKPVEAGHHHAKTGFFGWFNRGFARTAKNYEGWVARVLKRTGRAMIIY
                     AAVLGAVGLLYLRLPSSFLPNEDQGYAIMNVQLPPGATQGRTLSVMQQVEGFILKQPE
                     IDHIVSVLGFSFSGTGQNAALAFLPLKDWDERKGAQHTAQAVAGRAFGALMGVRDAFI
                     FALSPPPIPELGTSTGFNFRLEDRGGNGHDALVAARNQMLGMASQSKILAGVRPDGLE
                     DAPQLRVDIDRDQANALGVSFDSINATISTALGSAYVNDFPSQGRLQRVIVQADAAAR
                     MQPADLLKLNATNSQGQPVPLSSFA"
BASE COUNT          346 a          693 c          767 g          314 t
ORIGIN      
        1 gcgaaggagg acagcggcac cggctggccc tggctgttgg tcgcgttcag cttcagcaga
       61 tcggccggct gcatgcgagc cgccgcgtcg gcctggacga tgacgcgctg cagccgtccc
      121 tggctcggga agtcgttgac ataggccgag cccagcgcgg tggagatcgt cgcgttgatc
      181 gagtcgaagg acacgccgag cgcgttggcc tggtcgcggt cgatgtcgac gcgcagctgg
      241 ggcgcgtcct cgaggccatc cggccgcacg cccgcgagga tcttgctttg cgaggccatg
      301 cccagcatct ggttgcgtgc tgcgaccagc gcgtcatggc cattgccgcc gcgatcctcg
      361 agccggaagt tgaagccggt ggaggtgccg agctcgggaa tgggcggcgg actcagtgcg
      421 aagatgaagg catcgcgcac gcccatcagc gcgccgaagg cgcggcctgc cacggcctgg
      481 gccgtgtgct gcgcgccctt gcgctcgtcc cagtccttca gcggcaggaa ggcgagcgcc
      541 gcgttctggc cggtgcccga gaagctgaag cccagcacgc tgacgatgtg gtcgatctcg
      601 ggctgcttga ggatgaagcc ctcgacctgc tgcatcacgc tcaaggtgcg cccctgcgtg
      661 gcgccgggcg gcagctgcac gttcatgatc gcgtagccct ggtcctcgtt gggcaagaag
      721 gaggacggca gccgcagata gagcagcccg acggcgccga gcacggccgc gtagatgatc
      781 atggcccgcc cggtgcgctt gagaactcgc gccacccagc cctcgtagtt cttcgcggtg
      841 cgcgcgaaac cgcggttgaa ccagccgaag aagccggtct tggcgtggtg atggccggcc
      901 tccacgggct tgagcagcgt tgcgcacaag gccggcgtga gcgacagcgc catgaaggcc
      961 gacagcagga tggcggccac catcacggcc gagaactggc ggtagatgtt gcccaccgag
     1021 ccgccgaaga aggccagcgg cacgaacacc gaggccagca cgacggtgac gccgacgatg
     1081 gcgccctgga tctggcccat cgccttgcgc gtggccgcgc gcggcggcag gccttcctcg
     1141 ctcatgatgc gctcgacgtt ctccaccacg acgatggcgt cgtccaccac gatgccgatg
     1201 accagcacca tgccgaacat ggtgagcacg ttgatcgaga agcccagggc gagcagcacg
     1261 ccgaaggtgc ccatcagcgt gaccggcacg accatggtcg ggatcagggt gtagcggatg
     1321 tcctgcagga acaggtacat caccaggaac accagcacca cggcctcgaa cagggtctcg
     1381 accacttgcc tgatcgagat tctcacgaag cgcgagctgt cgtaagggat cgtggccttc
     1441 acgcccggcg ggaagtagcg cgacagatcc tccaccttct ggcgcaccgc cgtggccgtg
     1501 gccagggcgt tgccggtggg cgagagctgc acgccgatgc cggtggaggg gcggccgtcc
     1561 aggcgcgcct gggtggcata ggtctgggcg ccgagctcga tgcgcgccac gtcgcgcagg
     1621 cgcaccgagg agccgtcggc attggcgcgc agcacgatgt tgccgaactg ctcgatcgtg
     1681 ctgagctggc cacccaccgt cacggtggcg gagatggcct ggcccggctg cagcggcagg
     1741 ttgccgatgc cgccggcgct gacctgggcg ttctggccgc ggatcgcgtt gttgacctcg
     1801 gccgccgaga gtctgaagcc ggtcagcttg gccggatcga tccagatgcg catcgcgcgc
     1861 tcggtgccga acagctgggc ctggcccacg ccgggcacgc gctggatctc gggcagcagg
     1921 ttgcgcgagg cgtagtcgcc caaagcgatc gggtcatagg ccgggttgtc cgaggacagg
     1981 atcgcgaaca acaggaagtt ggagcgcgcc ttgtccacgc gcacgccctg ctgcgtcacg
     2041 gcggagggca ggcgcggcga ggcgcggctc agccggttct gcacgtccac cagggccagg
     2101 tcgggactgg tgcccggctc
//
LOCUS       DASUCY010000563         4975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1362226, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000563 DASUCY010000000
VERSION     DASUCY010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4975
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1362226"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="VFV25_11090"
     CDS             complement(<1..566)
                     /locus_tag="VFV25_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY1393915.1"
                     /translation="MRSTHPITGLVLTGGGARAAYQAGVLQAIVRIRRDCRAGRVRNP
                     FPVITGTSAGAINAAALACGADHFERAVERLAAVWRDFHAERIYRADSFGVIRSGAKW
                     LTMFSVGWALARWRRARPRSLLDNRPLEHLLNELVPLQRLPALLRGGQLRALAITASS
                     YSSGEHVIFYDAGDGIEPWTRSLRVAVR"
     gene            complement(644..1075)
                     /locus_tag="VFV25_11095"
     CDS             complement(644..1075)
                     /locus_tag="VFV25_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393916.1"
                     /translation="MKKLIACALFAAVAAPAMAADVGVSVSVSQPGLYGRIDIGNVPA
                     PAVILPQPVIIAPSPVAVYQQPIYLHVPPGHEKHWEKHCAQYNACGQPVYFVQEGWYQ
                     REYAPRFRNGRGDEDGDRGRGRGHGDRGDHGRKHGHGHEDD"
     gene            complement(1224..4781)
                     /gene="dnaE"
                     /locus_tag="VFV25_11100"
     CDS             complement(1224..4781)
                     /gene="dnaE"
                     /locus_tag="VFV25_11100"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEY1393917.1"
                     /translation="MSFVHLRTHTEYSVVDGTLRVDDVVAAAAADRQPALALTDLSNL
                     FGTVKFYREARGAGVKPIIGADVWLEPEGNDKQPSRLLLLIQNHAGYLNLCELLARGW
                     TQGVQRAQACLQWDWLAQLSGGLIALSGADQGAVGRALLAGDAARAESVAQRLASIFP
                     QRFFIELQRAGQASNEAHVRAAVPLAAKLKLPVVATHPVQFQTPEDFEAHEARVCIAE
                     GETLTNPRRVKRFTREQYFKSQAQMQALFADIPSAIANTELIAQACNLTLSLGKPQLP
                     DFPTPLADGQPMPMADYFRQLSHEGLNERLAHLYPDAAERERQRPAYVERLDFEIGTI
                     LKMGFPGYFLIVADFINWAKSNGCPVGPGRGSGAGSLVAYALKITDLDPLRYNLLFER
                     FLNPERVSMPDFDVDFCQGNRDRVIDYVKDKYGRDAVSQIATFGTMAAKAALRDVGRV
                     LGMGYGHVDSIAKLIPAPPGKTVTLKRPGDHPDPGLIYARKEAPEIEARERNEEEVAE
                     LLALAERVEGMVRNVGMHAGGVLIAPGKITDFCPLYMQPGSDSAVSQYDKDDVEAIGL
                     VKFDFLGLATLTILELAKDFIVARHPERAGFAFETLPLDDPQVYKLFSEGRTESVFQF
                     ESSGMQRMLRDAKPSRIEDLFALNAMFRPGPMENIPSFCARKHGREKVEYPHPLLETV
                     LGETYGVMVYQEQVMQAAQVLGGYSLGGADLLRRAMGKKKPEEMAKHRSIFREGAAKK
                     GIAEAKADEVFDLMEKFAGYGFNKSHAAAYSLLAYHTAWLKVHYPAEFFAATMTIELD
                     DADKLRVLINDAKQFGVNFDAPDVNAGVWRFEPVGDQRVPSPGGERGRVRYGLGAIKG
                     TGQGAIEAIVEARREGGAFKSFFDFCARVDRSRVNKRVAEALIKAGAFDALEPNRAAL
                     LASVGLGFEYAETQAAHADQGGLFDFGDGGGDAHGSSTQEPELVAAEPWSIRQRLTLE
                     KQALGFYLSGHLFDESEAEVRLFAKRKISDLIDSREPQLLAGIVGDLRIVNGQRGRVA
                     IFKLDDKSEAIEAVINEELLDASRELLKDDELLVVQGKLQPDRFSGGMRLNVQQLWDL
                     AGARCRFGRYLRVAVNGRTPPVADLLREFPPRREATEHGERVQGMSVRLALQRESPVA
                     GEVRGEIDLGEDARFYPSDSALARWRAEAPGRQAAIVYE"
BASE COUNT          777 a         1721 c         1652 g          825 t
ORIGIN      
        1 tggcgcacgg cgacgcgcag cgagcgcgtc cagggttcga tgccgtcgcc cgcgtcgtag
       61 aagatcacat gttcgcccga gctgtagctc gaggcggtga tcgccagcgc gcgcagctgg
      121 ccgccccgca gcaaggcagg caggcgctgc agcggcacca gctcgttcag caggtgctcc
      181 agcggtctgt tgtccagcag cgagcgcggc ctcgcgcgac gccagcgcgc cagcgcccag
      241 cccaccgaaa acatggtgag ccatttggcg ccactgcgga tcacgccgaa ggaatcggcg
      301 cgatagatcc gctcggcatg gaagtcgcgc cagaccgccg cgagccgctc gacggcccgc
      361 tcgaagtggt cggcgccgca agccagggcc gccgcattga tggcccccgc tgaagtgccg
      421 gtgatgaccg gaaatgggtt gcgcacccgc ccggcgcggc agtcgcggcg tatgcggacg
      481 atggcttgca gtacaccagc ctggtaggcc gcgcgagccc caccgccggt caggacgagg
      541 ccggtgatgg ggtgcgtgct gcgcatgcag cgcagtttag ggtcggccga caacctggac
      601 gatcaggatc ggccccgagg ctagttgaag cagccgacct cgctcagtca tcctcgtgac
      661 catggccatg cttgcgccca tggtcgcccc ggtcgccatg cccgcggcca cggccgcgat
      721 cgccatcttc gtcgccgcgg ccgttcctga agcgcggcgc gtactcgcgc tggtaccagc
      781 cttcctggac gaagtagacc ggctggccac aggcgttgta ctgagcgcag tgcttttccc
      841 aatgcttctc atgcccgggc ggcacatgca gatagatggg ctgctggtac accgcgaccg
      901 ggctcggcgc gatgatcacc ggctgcggca agatcaccgc cggcgcgggt acgttgccga
      961 tgtcgatgcg gccgtacaga ccgggctggc tgacgctgac cgaaacgccc acgtcggcgg
     1021 ccatggcagg cgctgcgacg gcggcaaaca aagcgcaggc aatcaatttc ttcatgcgat
     1081 cctccaagaa gttgtcggcc tactctcaac gtctcggtca ggcacgcgga cgacgccgcg
     1141 gctcaggccc aagatgaact ttcatcaagg actgtaaagc cctgcggcgg cacggattgt
     1201 cacagcttgt agcaaccgtg cggctattcg tagacgatag ccgcctggcg ccccggggcc
     1261 tcggcgcgcc agcgcgccag cgcgctgtcg ctggggtaga agcgcgcgtc ctcgccgagg
     1321 tcgatctcgc cgcgcacctc gcccgccacc gggctctcgc gctgcagggc cagcctcacg
     1381 ctcatgccct gcaccctctc accatgctcg gtagcctcgc gccgcggcgg aaactcgcgc
     1441 agcagatccg ccaccggcgg ggtgcggccg ttgaccgcca ccctcagata gcgcccgaag
     1501 cggcagcgcg cgccagccag atcccagagc tgctgcacgt tgaggcgcat gccgccgctg
     1561 aagcggtccg gctgcagctt gccctgcacg accagcagct cgtcgtcctt cagcagctcg
     1621 cggctggcgt cgagcaattc ctcgttgatc accgcctcga tggcttcgct cttgtcgtcc
     1681 agcttgaaga tcgccacgcg gccgcgctgg ccattgacga tgcgcagatc gccgacgatg
     1741 ccggccagca gctggggctc gcggctgtcg atcaggtcgc tgatcttgcg tttggcaaac
     1801 agcctcacct cggcctcgct ctcgtcgaag agatggcccg acaagtagaa gcccagggct
     1861 tgcttttcca gcgtcagccg ctgccggatg ctccagggct cggccgccac cagctcgggc
     1921 tcctgggtcg acgagccatg cgcatcgccg ccgccgtcgc cgaagtcgaa caggccgccc
     1981 tggtcggcat gggcggcctg cgtctcggca tattcgaagc ccaggcccac gctggcgagc
     2041 agcgccgcgc ggttgggctc cagcgcgtcg aaggcgcctg ccttgatcag cgcctcggcc
     2101 acgcgcttgt tgacgcggct gcggtccacg cgggcgcaga agtcgaagaa gctcttgaag
     2161 gcgccgccct cgcgcctggc ctcgacgatg gcctcgatcg cgccctgccc cgtgcccttg
     2221 atcgcgccca ggccatagcg aacgcggccg cgttcgccgc ccggtgacgg cacgcgctgg
     2281 tcgcccaccg gctcgaagcg ccacacgccg gcgttgacgt ccggcgcatc gaagttcacg
     2341 ccgaactgct tggcgtcgtt gatcagcacg cgcagcttgt cggcgtcgtc cagctcgatg
     2401 gtcatcgtcg cggcgaagaa ttccgccggg taatgcacct tgagccaggc cgtgtgatag
     2461 gcgagcagcg agtaggccgc cgcatggctc ttgttgaagc catagccggc gaacttctcc
     2521 atcaggtcga agacctcgtc ggccttggcc tcggctatgc ccttcttggc cgcaccctcg
     2581 cggaagatgc tgcggtgctt ggccatctcc tcgggcttct tcttgcccat cgcacggcgc
     2641 agcaggtcgg cgccgcccag cgaatagccg cccagcacct gcgccgcctg catcacctgc
     2701 tcctggtaga ccatcacgcc ataggtctcg cccagcacgg tctcgagcag gggatgcggg
     2761 tattccacct tctcgcgccc atgcttgcgc gcgcagaagc tcgggatgtt ctccatcggc
     2821 ccgggccgga acatcgcgtt cagcgcgaac aagtcttcga tgcggctggg cttggcatcg
     2881 cgcagcatgc gctgcatgcc gctggattcg aactggaaca ccgactcggt acgcccctcc
     2941 gagaacagct tgtagacctg cggatcgtcc agcggcagcg tctcgaaggc gaagcccgcg
     3001 cgctcggggt ggcgcgcgac gatgaagtcc ttggccagct ccaggatggt cagcgtggcc
     3061 aggcccagga agtcgaactt caccaggccg atcgcctcga catcgtcctt gtcgtactgg
     3121 ctcaccgccg agtcgctgcc cggctgcatg tagagcgggc agaagtcggt gatcttgccc
     3181 ggcgcgatca gcacgccgcc ggcatgcatg ccgacgttgc gcaccatgcc ctcgaccctc
     3241 tcggccagcg ccagcagctc ggccacctct tcttcgttcc tctcgcgcgc ctcgatctca
     3301 ggcgcctcct tgcgtgcata gatcaggccg ggatcggggt ggtcgccggg gcgcttgagc
     3361 gtcaccgtct tgccgggcgg cgcggggatc agcttggcga tgctgtccac atgcccgtag
     3421 cccatgccca gcacgcggcc cacgtcgcgc agggccgctt tggcggccat ggtgccgaag
     3481 gtggcgatct ggctcaccgc gtcgcggccg tatttgtcct tgacgtagtc gatcacgcgg
     3541 tcgcggttgc cctggcagaa gtcgacgtcg aagtcgggca tcgacacgcg ctcgggattc
     3601 aggaagcgct cgaacagcag gttgtagcgc agcgggtcga ggtcggtgat cttcagcgca
     3661 taggccacca gtgagccggc acccgagccg cgtcccggcc ccaccgggca gccgttgctc
     3721 ttggcccagt tgatgaagtc cgccacgatc aagaagtagc ccgggaagcc catcttgagg
     3781 atggtgccga tctcgaagtc caggcgctcc acataggccg ggcgctggcg ctcgcgctcg
     3841 gccgcatcgg gatagagatg ggcgagccgc tcgttcagcc cttcatgcga gagctggcgg
     3901 aaatagtccg ccatcggcat cggctggcca tcggcgagcg gggtcgggaa atccggcagc
     3961 tgaggcttgc ccaggctgag cgtgagattg caggcttgag cgatcagctc ggtgttggcg
     4021 atcgctgagg ggatgtcggc gaacaaggcc tgcatctgcg cctggctctt gaagtactgc
     4081 tcacgcgtga agcgcttgac acggcgcggg ttggtcagcg tttcgccctc ggcgatgcac
     4141 acccgcgcct catgcgcctc gaaatcctca ggcgtctgga actgcaccgg atgcgtcgcg
     4201 acgaccggca gcttgagctt ggcggccagc ggcacagcgg cgcgcacgtg ggcctcgttg
     4261 cttgcctgcc ccgcgcgttg cagctcgatg aagaagcgct gcgggaagat gctcgccagc
     4321 cgctgcgcca cgctctcggc acgcgccgcg tcacccgcca gcagcgcgcg gccgaccgcc
     4381 ccctggtcgg cgcccgacag ggcgatcaag ccgccgctca attgcgccag ccagtcccat
     4441 tgcaggcagg cctgggcacg ctgcacgccc tgcgtccagc cgcgcgccag cagctcgcag
     4501 aggttgagat agccggcatg gttctggatc agcagcagca gccggctcgg ctgcttgtcg
     4561 ttgccctccg gctccagcca gacatcggcg ccgatgatgg gcttgacacc tgcaccgcgt
     4621 gcctcgcggt agaacttgac ggtgccgaag agattgctga gatccgtcaa cgccagcgcc
     4681 ggctgccggt ccgccgccgc ggcggccacg acatcgtcga cgcgtagcgt gccgtcgacg
     4741 acggagtatt cggtgtgggt gcggaggtgg acgaaggaca taggcgggat tgtaggaagc
     4801 ggccttcccg ctgagcgaaa tgcaggtcgg ttcgatgcgt gggtggtttg ctcttatggc
     4861 tgctttatcg caccgagtgg cgccccgccg ggtccggccc ggctgccggg taagcccgcc
     4921 aggtgccgcg cctacggctc ggcctctcaa gcaaaccacg tcttgagatc aatgc
//
LOCUS       DASUCY010000564         2327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1555295, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000564 DASUCY010000000
VERSION     DASUCY010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1555295"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..791)
                     /locus_tag="VFV25_11105"
     CDS             complement(186..791)
                     /locus_tag="VFV25_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HEY1393918.1"
                     /translation="MPSYSLEGVVPVVDPSAYVHPTAVLIGDVIVGPGCYVGPNACLR
                     GDFGRIVLKAGSNVQDTCVIHGFPDADTVVEENGHIGHGAVLHSCVVRRDALVGMNAV
                     VMDEAEVGEQAIVAACAFVPAGMKIAPRSLVAGVPGKVLRELKDEEIAWKLEGTLTYQ
                     DLARRCLASLQEVTPLTAPEADRPQVKAPKVKPLIATRRGG"
     gene            972..1772
                     /locus_tag="VFV25_11110"
     CDS             972..1772
                     /locus_tag="VFV25_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEY1393919.1"
                     /translation="MSDESFVLVSQSGPVRSLTLNRPAALNSFTGPMHEQLLAALDEA
                     ATDNDVRCLVITGAGRGFCAGQDLSDPMAAPDPSGAPKDIGALIEKYYRPLALRLRTM
                     PVPVIAAVNGVAAGAGANIALGCDLVIAARSASFIQAFSKIGLLPDAGGTWLLPRLVG
                     RAQALGLALLGDKLPAEEAQRIGLIWKCVDDAALADEAQALAQRLAAMPVKALVSTRE
                     ALDAAEHLSFAQALDEEARRQRILGSAPDYQEGASAFLAKRAPRFTER"
     gene            1786..>2327
                     /locus_tag="VFV25_11115"
     CDS             1786..>2327
                     /locus_tag="VFV25_11115"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEY1393920.1"
                     /translation="MTSYDLNRDTRVAVIGAGSMGAGIAQLAAQAGHAVQLHDAQPGA
                     AARGLERIAADLDVAVKKGKLQSDERAVILGRIRIAERIEELEGAGLAVEAIVEKAEA
                     KQALFRALEAVLSPRAVFATNTSSISVTAVAQGLKHPQRLIGLHFFNPATRMKLVEII
                     RGVETDAALVPAMHALSKAWG"
BASE COUNT          359 a          803 c          796 g          369 t
ORIGIN      
        1 ctggctggcg aacgagtaca tcggacagac ctgcacgacg gcgcagtacc tcgccagcgg
       61 cggcagctgc tcggcgaccc gcaccgcgct cggcaactgg ggaacacgca tcagcaaggt
      121 cacgccctag gtgcactcgc atcgcgtcga gcaagcccgg cttggccggg cctgtttgtt
      181 cctgctcagc cgccccgcct ggtcgcgatc agcggcttca ctttcggtgc cttcacctgc
      241 ggccggtcgg cctcgggcgc ggtcagcggc gtgacctcct gcagcgaggc gaggcagcgc
      301 cgcgcgaggt cctgataggt cagggtgcct tcgagcttcc aggcgatctc ctcgtctttc
      361 agttcgcgca gcaccttgcc gggcacgccg gcgacgaggc tgcgcggcgc gatcttcatg
      421 ccggccggga cgaaggcgca ggccgcgacg atggcctgct cgcccacctc ggcctcgtcc
      481 atcacgaccg cgttcatgcc caccagcgcg tcgcgcctga cgacgcagct gtgcagcacg
      541 gcgccgtggc cgatgtggcc gttctcctcg accacggtgt cggcgtcggg aaagccgtgg
      601 atcacgcagg tgtcctgcac attggagccg gccttgagca cgatgcggcc gaagtcgccg
      661 cgcaggcagg cgttggggcc gacatagcag ccggggccga cgatcacgtc gccgatcagc
      721 accgcggtgg gatgcacata ggcgctgggg tcgaccacgg gcaccacgcc ctcgagggaa
      781 tagctgggca tctcgcttgt ctccggttcg ttcgttcggg tttttgctga gtcgctggaa
      841 cttgcgctgg atcaaacggg accataatat ctaccgaccg gtcggtcaat agtagttttg
      901 agccggatca agtgggccga gctacgcaaa ccctagcgcc agcccgcgcc gatctcccga
      961 ggagcccgtg tatgtccgat gaatcattcg tgctggtctc gcagtccggg ccagtgcgca
     1021 gcctcacgct gaaccggcct gccgcactga acagctttac tggccccatg catgagcagc
     1081 tgctcgctgc gctcgatgag gctgctacag ataatgatgt gcgctgcctc gtaataactg
     1141 gggcaggtcg cggtttttgt gccggacagg acttgtccga cccgatggct gcgcccgacc
     1201 ccagcggcgc gcccaaggac atcggcgcgc tgatcgagaa gtactaccgc ccgttggctc
     1261 tgcgcctgcg gaccatgccg gtgcctgtga tcgccgcggt caacggtgtg gcggccgggg
     1321 ccggggccaa catcgcgctc ggttgcgatc tcgtgatcgc ggcgcgctcg gccagcttca
     1381 tccaggcctt ctccaagatc ggcctgctgc ccgatgccgg cggcacctgg ctgctgccgc
     1441 gcctggtcgg ccgcgcccag gcgctgggcc tggcgctgct gggcgacaag ctgcccgccg
     1501 aggaagcgca gcgcatcggc ctgatctgga aatgcgtgga cgacgcggcg ctggccgacg
     1561 aggcccaggc cctggcgcag cgcctggccg cgatgccggt gaaggcgctg gtgtccacgc
     1621 gcgaggccct ggacgccgcc gagcatctga gcttcgcaca ggcgctcgac gaagaagcgc
     1681 ggcgccagcg catcctcggc tccgcgcccg actaccagga gggcgcgagc gccttcctcg
     1741 ccaagcgcgc cccgcgtttc accgagcgct aaggcaccgc atgtcatgac cagctacgac
     1801 ttgaatcgag acacccgggt cgccgtgatc ggcgccggca gcatgggcgc cggcatcgcc
     1861 cagctcgccg cgcaggcggg gcacgcggtg cagctgcacg atgcccaacc cggcgccgcg
     1921 gcgcgcggcc tggagcgcat cgcggccgac ctggatgtgg ccgtcaagaa gggcaagctg
     1981 cagtccgacg agcgtgcggt catcctgggc cgcatccgca tcgccgagcg catcgaggag
     2041 ctcgaaggcg cgggcctcgc ggtggaagcg atcgtcgaga aggccgaggc caagcaggcg
     2101 ctgttccgcg cactcgaagc ggtgttgagc ccgcgggcgg tgttcgccac caacacctca
     2161 tcgatctcag ttacggcagt ggctcagggc ctcaagcatc cgcagcggct gatcggcctg
     2221 catttcttca acccggccac gcgcatgaag ctggtcgaaa tcatccgcgg cgtcgagacc
     2281 gatgcggcgc tggtgccggc gatgcacgcg ctgtccaagg cctgggg
//
LOCUS       DASUCY010000565         3366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_526991, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000565 DASUCY010000000
VERSION     DASUCY010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526991"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1393
                     /gene="gspD"
                     /locus_tag="VFV25_11120"
     CDS             <1..1393
                     /gene="gspD"
                     /locus_tag="VFV25_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HEY1393921.1"
                     /translation="DQPGQNGAGGSNIFVVYLKNADATRLAQVLRAAYSATGSGGGAA
                     GGASTLGGAQSANYPATGVAAAGATSAIGGATGTGGTASVQATAPVQAAAQPSTGGFI
                     QADPATNSLIITAPEPVYRQLRAVIDQLDSRRAQVYIESLIVEMTVEKAAEFGFQWQG
                     LLGKKGDKTGVFGGTNFGTGGSNIVNLSLAAAGINTSGSSSSSTSITLPTGLNVGLIR
                     NYGGVYTLGALANFLNSQSDTNVLSTPNLIALDNEEAKITIGQNVPFVTGQFTSTGTG
                     GNLNPFQTIERKDVGVTLRVKPAIGENGTIRMTIYQENSSVVASTSSNTNGPTTNKSS
                     VETSVTLDDGQIMVLGGMMQDSVTTGVDKVPGLGDIPLVGNLFRNESRKHTKTTQLVF
                     LRPVVLRDADASSNITLDRYEAIRAEQAKQQPPHSVIMPINEVPVLPPLPAKPGATPA
                     APAPQPAASAPAN"
     gene            1431..2849
                     /gene="gspE"
                     /locus_tag="VFV25_11125"
     CDS             1431..2849
                     /gene="gspE"
                     /locus_tag="VFV25_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HEY1393922.1"
                     /translation="MRHPLPYSFAKANSLLLEDDGERLTLWADAGVSPAALSEVLRLY
                     AVDTVERESAATLAQRISAVYAGGESSAATVVGEVESAVDLSRMMQDLPAVEDLLEAA
                     NDAPIIRMLNALLTQAAKDGASDIHIEPYERSSSVRFRVDGTLREVVQPNKALHAALI
                     SRLKIMAELDIAEKRLPQDGRISLRIGGRAIDVRVSTLPSAHGERAVLRLLDKGDSSK
                     FSLESLGMSGDTLARFNRLIQQPHGIVLVTGPTGSGKTTTLYAALGRIDASTTNVLTV
                     EDPVEYELPGIGQTQVNPKIDLSFAKALRAILRQDPDVIMIGEIRDFETAQIAIQASL
                     TGHLVLATLHTNDAPSAVTRLTDMGIEPFLLSSSLLGVLAQRLVRKLCPACKRQDEAG
                     RWHPVGCPECLMSGYKGRTGVYELMVADSATQTLIHNRAAESELFTAAEAAGMKTMRA
                     DGERLVASGATSLEELLRVTRD"
     gene            2937..>3366
                     /locus_tag="VFV25_11130"
     CDS             2937..>3366
                     /locus_tag="VFV25_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEY1393923.1"
                     /translation="MPAYRFEALDANGKASTGLLEADNAKAARAQLRAQALVPLEVTA
                     VAAQPSDTGFSFGKRRVFTATGLAVWTRQLAGLVGSGLPLERALTALADEAEDPRQRE
                     LLAQLRAEVNAGSPFARALASAPREFDDVYRAVIGAGEQSG"
BASE COUNT          599 a         1228 c         1093 g          446 t
ORIGIN      
        1 agaccagccg ggccagaacg gcgccggcgg cagcaacatc ttcgtggtct acctgaagaa
       61 cgccgacgcg acccggctcg cccaggtgct gcgcgccgcc tacagcgcca ccggcagcgg
      121 cggcggcgcg gcgggcggtg cgtcgacgct gggcggtgcg cagagcgcca actacccggc
      181 gacgggtgtc gcagcagccg gcgcgacgag cgccatcggc ggcgccacgg gcaccggcgg
      241 caccgcctcg gtccaggcca cggcgccggt gcaggccgcg gcccagccgt cgaccggcgg
      301 cttcatccag gccgacccgg ccaccaattc gctgatcatc acggcccccg agccggtgta
      361 tcgccagctg cgtgccgtca tcgaccagct cgattcgcgg cgcgcccagg tctatatcga
      421 gtcgctgatc gtcgagatga cggtggaaaa ggcggccgag ttcggcttcc agtggcaggg
      481 cctcctgggc aagaagggcg acaagaccgg cgtgttcggc ggcaccaact tcggcaccgg
      541 cggcagcaac atcgtcaacc tgtcgctggc ggccgccggc atcaacacca gcggcagcag
      601 cagctcgagc acctccatca cgctgcccac cggcctcaac gtcggcctga tccgcaacta
      661 cggcggcgtc tacacgctgg gcgcgctggc caacttcctc aattcgcaga gcgacaccaa
      721 cgtgctgtcc acgcccaacc tgatcgcgct ggacaacgag gaagccaaga tcacgatcgg
      781 ccagaacgtg cccttcgtca ccggccagtt caccagcacc ggcaccggcg gcaacctcaa
      841 ccccttccag accatagagc gcaaggacgt gggcgtgacg ctgcgcgtga agccggcgat
      901 cggcgagaac ggcacgatac gcatgacgat ctaccaggag aactcctccg tggtggcgtc
      961 cacctcgagc aacaccaacg gccccacgac caacaagagc tcggtggaga ccagcgtcac
     1021 gctggacgac ggccagatca tggtgctggg cggcatgatg caggacagcg tcaccaccgg
     1081 cgtcgacaag gtgcccggcc tgggcgacat cccgctggta ggcaacctgt tccgcaacga
     1141 gagccgcaag cacaccaaga ccacgcagct ggtcttcctg cgcccggtcg tgctgcgcga
     1201 cgcggacgcc agcagcaaca tcacgctgga ccgctacgaa gcgattcgcg ccgaacaggc
     1261 caagcagcag cctccacaca gcgtgatcat gccgatcaac gaggtgccgg tgctgccgcc
     1321 tttgccggcc aagccgggcg cgacccccgc cgcgcctgcg ccccagcccg ccgcttcggc
     1381 gccggccaac tgaacgagcg cggccgggac aaaggagtac aaagatgggc atgcgacatc
     1441 ccctgcccta tagcttcgcc aaggccaaca gcctgctgct ggaggacgac ggcgagcggc
     1501 tcacgctgtg ggccgacgcc ggcgtctcgc ccgccgccct gtccgaggtc ttgcgcctct
     1561 acgccgtcga cacagtggag cgcgaatccg cggcgacgct tgcgcaacgc atctcggcgg
     1621 tctatgccgg cggcgaatcg agcgccgcga ccgtggtcgg cgaagtggaa agcgcggtcg
     1681 acctgtcgcg catgatgcag gacctgccgg cggtcgagga cctgctggaa gcggccaacg
     1741 acgcgcccat catccgcatg ctcaacgcgc tgctgacgca ggcggcgaag gacggcgcct
     1801 cggacatcca catcgagccc tacgagcgca gctcctcggt gcgcttccgc gtcgatggca
     1861 ccttgcgcga ggtggtgcag cccaacaagg ccctgcacgc cgcgctgatc tcgcgcctga
     1921 agatcatggc cgagcttgac atcgccgaga agcgcctgcc gcaggacggc cgcatctcgc
     1981 tgcgcatcgg cgggcgcgcg atcgacgtgc gcgtgtccac gctgccctcg gcgcacggcg
     2041 agcgcgccgt gctgcgcctg ctggacaagg gtgacagcag caagttctcg ctcgagtcgc
     2101 tgggcatgag cggcgacacg ctggcgcgct tcaacaggct gatccagcag ccgcacggca
     2161 tcgtgctcgt caccggcccc accggctcgg gcaagacgac cacgttgtat gccgcgctgg
     2221 gccgcatcga cgcctcgacc accaatgtgt tgacggtgga agacccggtc gaatacgagc
     2281 tgcccggcat cggccagacc caggtcaacc ccaagatcga cctcagcttc gcgaaggcgc
     2341 tgcgcgccat cctgcgccag gaccccgacg tcatcatgat cggcgagatc cgcgacttcg
     2401 agaccgcgca gatcgccatc caggcctcgc tgaccggcca cctggtgctc gccacgctgc
     2461 acaccaacga tgcgccctcg gccgtcacgc gcctcacgga catgggcatc gagcccttcc
     2521 tgctcagctc ctcgctgctg ggcgtgctgg cccagcgcct ggtgcgcaaa ctatgccccg
     2581 cgtgcaagag gcaggacgag gccggccgct ggcacccggt gggctgccca gaatgcctga
     2641 tgtcgggcta caagggccgc accggcgtct acgaactgat ggtggccgac tcggccacgc
     2701 agacactgat ccacaaccgc gctgccgaat ccgaactctt caccgccgcc gaagccgccg
     2761 gcatgaagac catgcgcgcc gacggcgaac ggctggtcgc cagcggcgcc acttcgctcg
     2821 aggaactgct gcgcgtcacc cgggattagc agtgaccagt gacctgtgac tggtgacttg
     2881 taaagaccaa ccacgcccag tcactagtca caagtcgcca gtcactaccc aaaaaaatgc
     2941 ctgcataccg cttcgaagcc ctggacgcca acggcaaggc cagcaccggc ctgctggagg
     3001 ccgacaacgc caaggcggcg cgtgcgcagt tgcgcgccca ggccctggtg ccgctggagg
     3061 tgacagcggt cgccgctcag ccgagcgata cgggcttctc cttcggcaag cgacgtgtat
     3121 tcaccgccac tggcctggcg gtgtggacgc gccagctggc tggcctggtc ggctcgggcc
     3181 tgccgctgga gcgcgccctg acggccctgg ccgacgaggc cgaggacccg cgccagcgcg
     3241 agctgctggc ccagctgcgc gccgaggtca atgccggttc gcccttcgcg cgcgcgctgg
     3301 ccagcgcgcc gcgcgagttc gacgatgtct atcgtgccgt gatcggcgcc ggcgagcaga
     3361 gcggcc
//
LOCUS       DASUCY010000566         2515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_800318, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000566 DASUCY010000000
VERSION     DASUCY010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800318"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VFV25_11135"
     CDS             complement(<1..644)
                     /locus_tag="VFV25_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEY1393924.1"
                     /translation="MSMNLVTLLYLVASVCFIQALKGLSHPTSSIRGNLFGMVGMAIA
                     MLTTAALIVEISGGKALGLGWVLLGLVVGGGYGAWRAKTVEMTKMPELVAFFHSMIGL
                     AAVFIAVAAVAEPYAFGIVAKGSPIPSGNRLELFLGAAIGAITFSGSVIAFGKLSGTY
                     KFRLFQGAPVQFAGQHKLNLILGLLTIGLGGVFMFTGNWPAFFAMLALAFVMGVL"
     gene            complement(641..979)
                     /locus_tag="VFV25_11140"
     CDS             complement(641..979)
                     /locus_tag="VFV25_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEY1393925.1"
                     /translation="MDAVSPTLINLIIFVLAIYVGYHVVWTVTPALHTPLMAVTNAIS
                     AIVIVGAMLAAALTETPLGKAMGVLAVALAAVNIFGGFLVTRRMLEMFRKKEKKVPAV
                     TPVAAEGAAK"
     gene            complement(1051..2166)
                     /locus_tag="VFV25_11145"
     CDS             complement(1051..2166)
                     /locus_tag="VFV25_11145"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEY1393926.1"
                     /translation="MLIGVPAEITAGETRVAITPETAKKLKSQGHTIRVQSGAGVAAS
                     VPDEAYTAVGAEITDAAGAYAADIVLKVRSPLDGEIAQARPGSVLVGMLNPFDAAGLQ
                     RLAAAGLTAFALEAAPRTSRAQSMDVLSSQANIAGYKAVMLAAEQYQRFFPMLMTAAG
                     TVKAARVVILGVGVAGLQAIATARRLGAVIEASDVRPSVKEQVESLGAKFIDVPYETD
                     EEREAAVGVGGYARPMPPSWLERQKAEVAKRVAQADIVITTALIPGRAAPVLVTEDMV
                     KSMKPGSVIVDLAAPQGGNCPLTEPNKIVIKHGVTLVGQTNVPALVAADASALYARNL
                     LDFLKLIISKEGALTIDLADDIVAACLMTQGGEVKRQ"
     gene            complement(2198..>2515)
                     /locus_tag="VFV25_11150"
     CDS             complement(2198..>2515)
                     /locus_tag="VFV25_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY1393927.1"
                     /translation="LFEASGNERALRGAFDALRPRGIIVQVGLGGEMNLPVNTIVAKE
                     FDLRGAFRFHEEFAIAVDLLNKGLVDVKPLISATMPYRDAGRAFALAADRSQAMKVLL
                     SFD"
BASE COUNT          413 a          824 c          848 g          430 t
ORIGIN      
        1 agcacgccca tcacgaaggc cagcgccagc atcgcgaaga acgcgggcca gttgccggtg
       61 aacatgaaga ccccgcccag gcctatggtc agcaggccca ggatcaggtt cagcttgtgc
      121 tggccggcaa actgcaccgg cgcgccctgg aacaggcgga acttgtaggt gcccgacagc
      181 ttgccgaagg cgatgaccga accgctgaag gtgatggcgc cgatggccgc gcccaggaac
      241 agctcgagcc ggttgccgct ggggatgggg ctgcccttgg ccacgatgcc aaaggcgtag
      301 ggctcggcga ccgccgccac ggcgatgaac accgcggcca ggccgatcat gctgtggaag
      361 aaggcgacca gctcgggcat cttggtcatt tcgaccgtct tggcgcgcca ggcgccatag
      421 cccccgccga ccaccaggcc cagcagcacc cagcccagcc ccaaggcctt gccgccggag
      481 atttcgacga tcagcgccgc cgtggtcagc atggcgatgg ccatgcccac catgccgaac
      541 aggttgccgc ggatcgagct ggtggggtgc gacaggccct tgagcgcctg aatgaagcag
      601 accgaggcca ccaggtacag cagcgtgacg aggttcatgc tcatttggcc gctccttcgg
      661 ccgcaaccgg ggtcaccgcc ggcactttct tttctttctt cctgaacatt tccagcatcc
      721 ggcgcgtcac cagaaagccg ccgaagatgt tgacggccgc cagcgccacg gccagcacgc
      781 ccatggcttt gcccagcggc gtctcggtca gcgcggcggc cagcatggcg cccacgatca
      841 cgatggccga gatcgcgttg gtcacggcca tcagcggcgt gtgcagcgcc ggcgtgacgg
      901 tccagaccac gtggtagccc acgtagatgg cgaggacaaa aataatcagg ttgatgaggg
      961 tgggtgacac ggcatccatg gttttctcct gtgagggcgg cccttgggcc agcccaaaaa
     1021 tgacgggttg gtgttgactt cgccgcaggc ttattggcgt ttgacttcgc cgccctgcgt
     1081 catcaggcag gccgccacaa tatcgtccgc caggtcgata gtgagcgcgc cttccttgct
     1141 gatgatgagc ttgaggaagt cgagcaggtt gcgggcatag agcgcgctgg catcggccgc
     1201 gacgagggcc ggcacattgg tctggccgac cagcgtcacg ccgtgtttga tcacgatttt
     1261 gttgggctcg gtcaaggggc agttgccgcc ctgcggtgcg gccaggtcga cgatcacgct
     1321 gccgggcttc atggacttga ccatgtcttc ggtgaccagc acgggcgcgg cgcggcccgg
     1381 gatcagggcg gtggtgatga cgatgtcggc ctgcgccacg cgcttggcga cctcggcctt
     1441 ctggcgctcc agccagctcg gcggcatggg cctggcgtaa ccgcccacgc cgacggcggc
     1501 ttcgcgctct tcgtcggttt cgtagggcac gtcaatgaac ttggcgccca gcgattcgac
     1561 ctgctccttg acgctggggc gcacgtcgct ggcttcgatc acggcgccca ggcgcctggc
     1621 ggtggcgatg gcctgcaggc ccgccacccc cacgcccagg atcaccacgc gggccgcttt
     1681 gacggtgccg gcggccgtca tcagcatggg gaagaagcgc tggtactgct cggcggccag
     1741 catcacggcc ttgtagccgg caatattggc ctgcgaggag agcacgtcca tgctttgcgc
     1801 gcggctggtg cgcggcgcgg cttccagcgc aaacgcggtc agtccggccg ccgccagccg
     1861 ctgcaggccc gcggcatcaa aggggttgag catgccgacc agcacgctgc ccggcctggc
     1921 ctgcgcgatt tcgccgtcca gtgggctgcg caccttgagc acgatgtccg cggcataagc
     1981 gccggccgcg tcggtgatct cggcgccgac ggcggtgtag gcctcgtcgg gcacgctggc
     2041 cgccacgccg gcgcccgact ggacgcggat ggtgtggccc tggcttttaa gctttttggc
     2101 cgtttccggc gtgatggcaa cgcgtgtttc tccggccgtg atttcggcgg gtaccccaat
     2161 cagcatgatg atttcctttg aaagactttg ggggtattta atcgaagctg agcaacactt
     2221 tcatggcctg cgagcggtcg gcggccaagg cgaaagcgcg gcctgcgtcg cggtagggca
     2281 tggtggcgga gatcagcggc ttcacatcca ccagcccctt gttgagcaag tccacggcga
     2341 tggcgaactc ctcatggaag cgaaacgccc cgcgcaggtc gaactccttg gccacgatgg
     2401 tgttgaccgg caggttcatt tcgcccccca gccccacctg cacgatgatg ccgcgcgggc
     2461 gcagcgcgtc gaacgccccg cgcagcgcgc gctcgttgcc gctggcctcg aagag
//
LOCUS       DASUCY010000567         3930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_591447, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000567 DASUCY010000000
VERSION     DASUCY010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591447"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..488)
                     /locus_tag="VFV25_11155"
     CDS             complement(171..488)
                     /locus_tag="VFV25_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEY1393928.1"
                     /translation="MKQLEVTIMGQSYILGCPDGGETALLQAVGEVDREMSAIRDAGK
                     VKARERIAVLAALNLAYALAERPAAPAPATAGPQDPQAAAAEVDALIRKLDAALGEDG
                     RLL"
     gene            complement(485..697)
                     /gene="zapB"
                     /locus_tag="VFV25_11160"
     CDS             complement(485..697)
                     /gene="zapB"
                     /locus_tag="VFV25_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HEY1393929.1"
                     /translation="MSRIAELAERVERLLLRHEELKRTNALLEQELAAVKEERDSLRS
                     RLAAARARIDALLERLPADPPKEERA"
     regulatory      759..1011
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1083..3002
                     /locus_tag="VFV25_11165"
     CDS             1083..3002
                     /locus_tag="VFV25_11165"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY1393930.1"
                     /translation="MFNSPAVRRAPVALAAAAACLSLGLLPSLARAQAESSLPQVVVT
                     ATRTPTPIDRVVADVVVIDEAQIRDAAGSSVEELLRQQAGVQILRNGGPGQNASVLIR
                     GASAQNTVVLVDGVRVGSATLGLAEFEALSLAQIERIEILRGPGSSLYGADAVGGVVQ
                     IFTRRGASGAPRLQASVLAGRYGSREASAGVSGASDAFDYAASYSREGSNGVSAISNP
                     ANPAYNPDTDGYGRRSASLQLGYAPVEGQRIGLKWLSSRLLSHFDSDEYPPPNFTPDP
                     SPDFRNWLNTDVLALDYSARFTKAWQSQLSWSDSKDSLRSGGNTISQYDTKRQQLGWQ
                     NTFTLAPGQTLVGLAEHLEETVDAASISGSELKRRNNALALSYTGSAESHRWQADIRH
                     DGNSAYGSQTTGKLGYAYQISRPLSVRASAGTAFRAPTFNDLYYPGYGVTTIRPERSR
                     SIELGANLREADWNAQFTWYRNRVSDMIGYQPVATQCPDPVTYAFGCAANIGKARLQG
                     ASLAVQGHVQAWQWSLGAEFLDAKDLVTGQRLLRRAAHTETASLGYRTETWGANAAVS
                     SVGARPDVDSNFSQVQLPSYALVDLSAYYAFARQWRFETKLLNAFDKRYEPVKDYGSL
                     GRSFWIGLRYDTLGF"
     gene            3010..>3930
                     /locus_tag="VFV25_11170"
     CDS             3010..>3930
                     /locus_tag="VFV25_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEY1393931.1"
                     /translation="MRPPPRLALWLTMAVATAFAALALGLAVGSSGWGWADAQIVWQI
                     RAPRVLAGFGAGAALALAGALMQLLTRNALADPYVLGVSGGASVGALLALLLAPLAWS
                     FWAPALGAALGALLATALLFALAWRALGRANVLGSAGEGSASLLLIGVMLGAAASALI
                     SLLLTLAPDLQLRGMVFWLLGDLNGATEWRPVWLALALGLALVWPRAHELDLLARGEA
                     WAASLGVPVAQRRRLALLAAALMTGAAVATTGAVGFVGLVMPHLMRLAGLRAAAVLLP
                     ASALAGGIFLCLADTAARTIVAPLQLPVGVL"
BASE COUNT          551 a         1350 c         1345 g          684 t
ORIGIN      
        1 ggggattcag gtgggcgagg tcagtaaggc ttcgggttca tctaacgcga gacgatcaca
       61 cctgccagac gatttccagg cccttgctca atgagcatcg gttcaaggaa atataagact
      121 cacgcgaacg cgacggacga gtggattcta gacctgcggc gtatcgggga tcacaacagt
      181 cggccatcct cgccgagcgc ggcgtcgagc ttgcggatca gcgcgtcgac ctcggctgcg
      241 gcggcctggg gatcctgcgg cccggccgtt gccggggctg gcgcggcggg ccgctcggcc
      301 aatgcatagg ccaggttgag cgcggccagc accgcgatgc gctcgcgcgc cttcaccttg
      361 ccggcatcgc gtatcgcaga catctcgcga tcgacttcgc cgaccgcctg gagcagggcc
      421 gtctcgccgc cgtcggggca gccgaggatg tagctctgcc ccatgatcgt gacctcaagc
      481 tgtttcacgc gcgctcctct ttcggcgggt cggccggcag gcgctccagc aatgcatcga
      541 tgcgcgcgcg cgccgcggcc aggcgcgagc gcagcgagtc gcgctcctcc ttcacggcgg
      601 cgagttcctg ttccagcagg gcgttggtgc gcttgagttc ttcatgccgc agcagcaggc
      661 gttccacgcg ttcggcaagc tcagcgattc tcgacatcga tcccccaggc cttggccatc
      721 aaggcttgtc gttcgattgt aggggggctg ttgcccgcct ttacaatcaa agcgttggtg
      781 ccccgtgccg gtgttcacgc cggcgcagtt aaacgggaag cagggcggtc gctgtttcgc
      841 atcaaagaga caacgcgacc caacctgcgc tgcccccgca acggtaagcg gacgggattg
      901 tttccagtcc tcgctcgtgt gcacaagcca ctggaggcct cttgagtctc tgggaaggtc
      961 gcacgggtcg ctccgccagc ccggataccg gccaacgggt ggcgcgaggc ccatgggtct
     1021 tggcgccgta ttgcatgcgc ctgcggggaa gcacggcgct gcgccgttct cgcttcattc
     1081 ccatgttcaa ttcccctgcg gtgcgtcgag cacctgtcgc gctggctgct gctgcggcct
     1141 gcctttccct tggtcttctt ccgtccctcg ctcgcgcgca agccgagtcc agcctgcccc
     1201 aggtcgtggt gacggctacg cgcacgccca cgcccatcga ccgcgtggtg gccgatgtcg
     1261 tcgtcatcga tgaggcgcag atccgcgatg ccgccggcag cagcgtcgag gagctgctgc
     1321 gccagcaggc cggcgtgcag atcctgcgca acggcggccc cggccagaac gcttcggtgc
     1381 tgatacgcgg cgcatcggcg cagaacacgg tggtgctggt cgacggcgtg cgggtcggtt
     1441 cggccacgct gggcctggcc gagttcgagg cgctgagcct cgcgcagatc gagcgcatcg
     1501 aaatcctgcg cggcccgggc agcagccttt atggtgcgga cgccgtgggc ggtgtggtgc
     1561 agatcttcac gcgccgtggc gccagcggcg cgccgcgcct gcaggcctcc gtgctcgccg
     1621 ggcgctacgg ttcgcgcgag gccagcgccg gcgtgagcgg cgcgagcgat gccttcgact
     1681 atgcagcctc ttacagccgc gaaggcagca atggcgtctc ggccatcagc aaccccgcca
     1741 acccggccta caaccccgac accgacggct acggtcggcg cagcgcttcg ctgcagctgg
     1801 gctatgcgcc ggtggagggc cagcgcatcg gcctcaagtg gctgagcagc cgtttgcttt
     1861 cgcacttcga ttccgacgaa tatccgccgc ccaacttcac ccccgacccg agcccggact
     1921 tccgcaactg gttgaacact gacgtgctgg cactcgacta cagcgcgcgc ttcaccaagg
     1981 cttggcaaag ccagctgagc tggtccgaca gcaaggacag cctgcgctcg ggcggcaaca
     2041 cgatcagcca gtacgacacc aagcgtcaac agctcggctg gcagaacacc ttcacgctgg
     2101 cgccaggcca gaccctggtc ggcctggccg agcatctgga ggagaccgtc gatgccgcct
     2161 ccatcagcgg cagcgaactg aagcgccgca acaacgcgct ggcgctgagc tacaccggca
     2221 gcgccgagtc gcaccgctgg caggccgaca tccgccatga cggcaacagc gcctatggca
     2281 gccagaccac gggcaagctc ggctacgcct accagatcag ccggcccttg tcggtgcgcg
     2341 cttctgcggg cacggccttc cgtgccccga ccttcaatga tctctactac cccggctacg
     2401 gcgtgacgac gatccgcccg gagagatcgc gcagcatcga gctcggcgcg aacttgcgcg
     2461 aggccgattg gaacgcgcag ttcacgtggt accgcaatcg cgtcagcgac atgatcggct
     2521 accagccggt cgccacgcaa tgcccggacc cggtgaccta tgccttcggc tgcgccgcca
     2581 acatcggcaa ggcgaggctg cagggcgcat cgctggcggt gcagggccat gtacaggcat
     2641 ggcaatggag cctgggtgcc gagttcctcg acgccaagga tctcgtcacc ggccagcgcc
     2701 tgctgcgccg cgccgcgcac accgagaccg cgagcctggg ctaccgcacc gagacctggg
     2761 gtgcgaacgc cgccgtcagt tcggtcggcg cgcggcccga cgtggactcc aacttcagcc
     2821 aggtgcagct gccttcctat gcgctggtcg acttgagcgc ctattacgcc ttcgcgcgtc
     2881 aatggcggtt cgagaccaag ttgctgaatg cgttcgacaa gcgctatgag ccggtcaagg
     2941 actatggttc gttgggccgc agcttctgga tcggcctgcg ctacgacacc ctgggcttct
     3001 gagtctgcaa tgcgtccgcc gccgcgcctc gccctctggc tgactatggc cgtcgcgacg
     3061 gccttcgcag cgctggcgct gggcctggcg gtgggctctt ctggctgggg ctgggcggat
     3121 gcgcagatcg tctggcagat ccgcgcgccg cgcgtgctgg ccggcttcgg cgctggcgcg
     3181 gcgctcgcgc tggccggcgc gctgatgcag ctgctgacgc gcaatgccct ggccgaccct
     3241 tacgtgctcg gcgtgtcggg cggcgcctcg gtaggcgcgc tgctggcctt gttgctcgcg
     3301 ccgctggcct ggagtttctg ggcgcctgcc ctcggtgccg cgctgggcgc tttgctggcc
     3361 accgcgctgc tgtttgcgct cgcctggcgt gcgttgggtc gagccaatgt gctgggctct
     3421 gcaggcgagg gcagcgcgag cttgctgctg atcggcgtga tgctgggcgc cgccgcttcg
     3481 gcgctgattt cgctgctgct cacgctggcg ccggacttgc agctgcgcgg catggtgttc
     3541 tggctgctgg gcgatttgaa cggcgcgacc gaatggcggc ctgtctggct cgcgctggcg
     3601 ctgggcttgg cgctggtctg gccgcgtgcg cacgagctgg acctgctcgc gcgcggcgag
     3661 gcctgggccg cgagcctggg cgtgccggtg gcgcagcggc ggcgcttggc gctgctggcg
     3721 gcggccttga tgaccggagc ggccgtcgcg actacgggcg ccgtcggctt cgtcggcctc
     3781 gtgatgccgc atctgatgcg gctcgccggg ctgcgtgccg ccgccgtgct cctacctgca
     3841 agcgcattgg ccggcggcat cttcctatgc ctggccgaca cggcggcgcg caccatcgtc
     3901 gcgccgctgc agctaccggt gggcgtcttg
//
LOCUS       DASUCY010000568         2855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_45572, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000568 DASUCY010000000
VERSION     DASUCY010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45572"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..525
                     /locus_tag="VFV25_11175"
     CDS             118..525
                     /locus_tag="VFV25_11175"
                     /inference="COORDINATES: protein motif:HMM:NF022592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2917 domain-containing protein"
                     /protein_id="HEY1393932.1"
                     /translation="MNTLTMNELQQSGQASWRLAPGQSSTLKAAGDTRWLRVCEGKLW
                     VTTAGRLGVEIPDDAWLQQGEVLELPAGVSAVLEGWPQASFQVLEAAPGTAPRQDFDL
                     LRGAARLTADFLAGLAARARSAASSPSRAHGAI"
     gene            complement(408..806)
                     /locus_tag="VFV25_11180"
     CDS             complement(408..806)
                     /locus_tag="VFV25_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEY1393933.1"
                     /translation="MKKPQDNEYVAYCLELLSSLGPCNARSMFGGYGIYLDGLMFALI
                     AADRLYLKADADSEAQWRSAAGEPFVYQSPKGPMAMSYWTPPQEAMDSPAQMAPWARL
                     GLEAALRARAAKPAKKSAVKRAAPRSKSKS"
     gene            927..1541
                     /locus_tag="VFV25_11185"
     CDS             927..1541
                     /locus_tag="VFV25_11185"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEY1393934.1"
                     /translation="MPLPFDASLPSRDKPTLRRLLQAERLGLVDRHARAMQLQDVLRI
                     WLTQRPDVTIGAYWPIKGEFDPLPALYRWTEGAGKGEEGAGPPRRIGLPVINRETKQL
                     RFHAWYPGCPMENDAYDIPKPKGTDVLLPTLLLVPCVGFGPRGVRLGYGGGFYDRTLA
                     ALKPRPYTVGLGYAHGFVPWLEAEPHDVPLDAMLTEEGVDWERT"
     gene            1628..2587
                     /locus_tag="VFV25_11190"
     CDS             1628..2587
                     /locus_tag="VFV25_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY1393935.1"
                     /translation="MSFSFLRRALLAFGLAAVALSAQAGAPLQKSQVPGWYRVMVGDF
                     EVTALYDGYIDLDPKALLKFTHPGEIERGMARAYEPGPQVQTSVNAFLVNTGSQLILV
                     DTGAAKAFGPALGFLVDNLKASGYDPAQVDLILLTHLHGDHVNGVLSPEGQIAFPNAK
                     VMVSAPESAFWLSDAMKQQAPKEAQPFFDMAQKAAAPYQAAGRWTLFEPNAQIAPGVK
                     AVATGHTPGHSSYLFESKGQQLLVMGDMIHVGAVQFARPEVAMSFDTDNKPAIASRKL
                     WFAKAAKDKMLLAGAHLSFPGLGHVRAEGKGYVWVPVEYSPLR"
     gene            complement(2641..>2855)
                     /locus_tag="VFV25_11195"
     CDS             complement(2641..>2855)
                     /locus_tag="VFV25_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HEY1393936.1"
                     /translation="VAALNPSIKKDSWGLAVGAGVDIPFGNEGWLFNVDLKKVQIGTD
                     VYSSGTKVGSFKVDPVLFSIGIGKRF"
BASE COUNT          467 a          996 c          929 g          463 t
ORIGIN      
        1 ttctattaat gcgattccgt catcaatcat gcctccgatc tcattggacc gccagtgtct
       61 tttcaccgaa cattcactgc acaacgagat gatcactgcg ccacaaggag gccaatcatg
      121 aacaccttga ccatgaatga attgcaacaa tccgggcagg catcctggcg tctggcgccg
      181 ggccaatcga gcacgctgaa ggccgcgggc gacacgcgct ggctgcgcgt atgcgaaggc
      241 aagctgtggg tcaccacggc cggtcgcctg ggcgtcgaga tccccgacga tgcttggctg
      301 cagcagggcg aagtgctgga gctgccggcc ggcgtcagcg ccgtgctgga aggctggccg
      361 caggcgagct tccaggtgct ggaggcggcc cccggtaccg cgccgcgtca ggacttcgac
      421 ttgctacgcg gtgctgcgcg cttgacggcc gactttttgg ccggcttggc ggcccgcgcc
      481 cgcagcgcgg cctccagccc cagccgcgcc cacggcgcca tctgagccgg cgaatccatg
      541 gcctcctgcg gcggcgtcca gtagctcatc gccatcggcc ccttagggct ctggtagacg
      601 aagggctcgc cggccgcgga gcgccactgc gcttcgctgt ccgcatcggc cttgagataa
      661 agccgatcgg cggcgatgag cgcgaacatc aggccgtcga gatagatgcc gtagccgccg
      721 aacatgctgc gcgcattgca cgggccgagc gaggacagca gctccaggca ataggcgacg
      781 tattcgttgt cctgcggctt cttcatatcg gctccggctt ctgctgacga cgctctttgc
      841 ttgaaggcaa ggctcgcacg ataaggcatg cgcgcgccgc aggcacaatc gacccacgcc
      901 ttccggcgct gagaaccgag gccgcgatgc ccctgccctt cgatgccagc ctcccttccc
      961 gcgacaagcc cacgctgcgc cgcctgctgc aggccgagcg cctggggctg gtcgaccgcc
     1021 acgcgcgcgc gatgcagctg caggacgtgc tgcgcatctg gctcacccag cgccccgacg
     1081 tgaccatcgg ggcctattgg ccgatcaagg gcgagttcga tccgctgccg gcgctgtacc
     1141 gctggaccga gggcgccggc aagggcgagg aaggcgccgg cccgccacga cgcatcgggc
     1201 tgccggtcat caaccgcgag accaagcagc tgcgctttca cgcctggtat cccggctgcc
     1261 cgatggagaa cgacgcctac gacatcccca agcccaaggg caccgacgtg ctgctgccca
     1321 cgctgctgct ggtgccctgc gtgggcttcg gccccagggg cgtgcgcctg ggctatggcg
     1381 gcggcttcta cgaccgcacg ctggccgcgc tcaagccccg cccctacacc gtgggcctgg
     1441 gctatgcgca tggcttcgtg ccctggctgg aggccgagcc gcacgacgtc ccgctggacg
     1501 cgatgctgac cgaggaaggg gtggactggg agcggacctg agcgcgcaga ggctgaacta
     1561 acggggcttc tgcaaggccg gcccaaaccg gctatcgtgc gcgctctcaa aacgagagga
     1621 ggtcctcatg tccttttcct tcctgcgccg cgcgctgctc gcgttcggcc tcgccgctgt
     1681 tgccttgtcc gcccaggccg gcgcgccgct gcagaagtcc caggtgccgg gctggtaccg
     1741 cgtcatggtc ggcgacttcg aagtcaccgc gctgtacgac ggctacatcg atctcgatcc
     1801 caaggcactg ctgaaattca ctcaccccgg cgagatcgag cgcggcatgg cgcgcgccta
     1861 tgagccgggg ccgcaggtgc agacctcggt caacgccttt ctggtcaaca ccggcagcca
     1921 gctgatcctg gtcgacaccg gcgcggccaa ggccttcggc ccggcgctgg gcttcctcgt
     1981 cgacaatctc aaggcctcgg gctacgaccc ggcgcaagtc gacctgatcc tgctcacgca
     2041 tctgcacggc gaccacgtca acggcgtgct gagccccgag ggccagatcg ccttcccgaa
     2101 cgcgaaggtc atggtcagcg cgcccgaaag cgcgttctgg ctcagcgatg cgatgaagca
     2161 gcaggcgccg aaagaggcgc agcccttctt cgacatggcc cagaaggccg ccgcgcccta
     2221 ccaggcggcc ggccgctgga cgctgttcga gcccaatgcg cagatcgcgc cgggcgtgaa
     2281 ggcggtcgcc accggccaca cgccgggcca cagcagctat ctgttcgagt ccaagggcca
     2341 gcagctgctg gtgatgggcg acatgatcca cgtcggcgcc gtgcagttcg cgcgccccga
     2401 ggtggccatg agcttcgaca ccgacaacaa gccggcgatc gcctcgcgca agctgtggtt
     2461 cgcgaaggcg gcgaaggaca agatgctgct cgccggcgcc caccttagct tccccggcct
     2521 cggccatgtg cgggccgagg gcaagggcta tgtctgggtg ccggtggagt attcgccgct
     2581 gcgctgaagc ccggctactc tgtggcgggc tcggcttagc gagcccgccc acgctttagc
     2641 tcagaagcgc ttgccgatgc cgatgctgaa cagcaccggg tccaccttga agctgccgac
     2701 cttggtgccc gacgaataga cgtcggtgcc gatctgcacc ttcttcaagt cgacattgaa
     2761 cagccagccc tcgttgccga aggggatgtc gacgcccgcg cccacggcga ggccccagct
     2821 gtccttcttg atgctcgggt tgagtgccgc tacca
//
LOCUS       DASUCY010000569         2022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1507409, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000569 DASUCY010000000
VERSION     DASUCY010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1507409"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="VFV25_11200"
     CDS             <1..551
                     /locus_tag="VFV25_11200"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEY1393937.1"
                     /translation="VAMLRGFVAGQADAPASIAGALAQGDAATAERLAHTLKGLAGNI
                     GAAGLQQQAAALEQAIREAAATQAPQAEVEAHLKILIAAIQAALPPERREEAAPQAAV
                     DPAQRERVFKQLAAMLADDDAQAERYLGEQAGLLRAALGTRFAALEAAVKRYEYEQAL
                     ALLNEPQPDSSSSHKQASEVRA"
     gene            548..1705
                     /locus_tag="VFV25_11205"
     CDS             548..1705
                     /locus_tag="VFV25_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator"
                     /protein_id="HEY1393938.1"
                     /translation="MNAITCLTAKPIILVVDDTPENLSLVGDLLAEDYRVKVAPSGAR
                     ALKIAYGETPPDLILLDIMMPEMDGYEVCRRLKADPATAEVPIIFLTAKGETEDERLG
                     FELGAVDYITKPISPPILLARVKTHLTLKASADFLRDKNAFLEQEVAKRTEEVRLVQD
                     VTILALASLAETRDNETGNHILRTQHYVRVLAQKLRDHPRFKDFLTERNIELLFKSAP
                     LHDIGKVGIPDRILLKPGHLEAPEFEIMKTHTTQGRDAIEHAEKRLGKSVDFLHFAKE
                     IAYSHQEKWDGSGYPQGLAGDAIPIAARLMAIADVYDALISRRVYKEPMPHEQAIVLI
                     EEGRGKHFDPDVVDAFLSIQDECRAIAARYLDTHADLDAEIDRLEHIAERM"
     gene            1748..>2022
                     /locus_tag="VFV25_11210"
     CDS             1748..>2022
                     /locus_tag="VFV25_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393939.1"
                     /translation="MDASAAEPAEPVDAPPAEPAESAAAEPGPAEPAFAEPPTLEPAA
                     ADLAALEPAEAAPRRSRKALVLKSALAVLLAGLLVQLVSWELSTSTL"
BASE COUNT          374 a          687 c          673 g          288 t
ORIGIN      
        1 atgtggcgat gctgcgcggc ttcgtcgccg gccaggccga tgcgcccgct agcatcgccg
       61 gggccctggc ccaaggcgac gcggcaacgg ccgagcggct ggcccacacc ttgaagggcc
      121 tggccggcaa catcggcgcc gccggcctgc agcagcaggc cgccgcgctg gagcaggcga
      181 tacgcgaagc cgccgccacg caggcgccgc aggccgaagt cgaggcgcat ctgaagatct
      241 tgatcgccgc catccaggcg gcgctgccgc ccgagcggcg ggaagaggcc gcgccgcagg
      301 cggcggtaga ccccgcgcag cgcgaacgcg tcttcaagca gctggccgcg atgctggcag
      361 acgacgatgc acaggccgaa cgctacctgg gcgagcaggc cggcctgctg cgggccgcgc
      421 tgggcacgcg cttcgcggcc ctggaggcgg ccgtcaagcg ctacgagtat gagcaggccc
      481 tggcgctgct gaatgaaccc cagcccgact cttcttcttc gcacaagcaa gcaagcgagg
      541 tgcgcgcatg aatgccatca cctgcctcac cgccaagccc atcatcctcg tggtggacga
      601 cacgcccgaa aacctgtcgc tggtaggcga tctgctggcc gaggactacc gcgtcaaggt
      661 cgcgcccagc ggcgcgcggg ccctgaagat tgcctacggc gagacgccgc ccgatctgat
      721 cctgctcgac atcatgatgc cggagatgga cggctacgag gtctgcaggc ggctgaaggc
      781 cgacccggcc acggccgagg tccccatcat ctttctcacc gccaagggcg aaaccgagga
      841 cgagcggctc ggcttcgagc tgggcgcggt ggactacatc acgaagccga tcagcccgcc
      901 catcctgctg gcgcgggtca agacccatct gacgctgaag gcctcggccg acttcctgcg
      961 cgacaagaac gcctttctgg aacaggaggt ggccaagcgc accgaggaag tgaggctggt
     1021 gcaggacgtg accatactcg cgctggcctc gctggccgag acgcgcgaca acgagacggg
     1081 caaccacatc ctgcgcaccc agcactacgt gagggtgctg gcccaaaagc tgcgcgatca
     1141 tccgcgtttc aaggacttcc tgaccgaacg caacatcgag ctgctgttca agtcggcccc
     1201 tttgcacgac atcggcaagg taggcatccc ggatcgcatc ctgctcaagc cggggcatct
     1261 ggaagcgcct gagttcgaga tcatgaagac tcacacgacg cagggccggg acgcgatcga
     1321 gcatgccgag aagcgcctgg gcaagagcgt ggacttcctg cacttcgcca aggagatcgc
     1381 ctattcgcac caggagaagt gggacggcag cggctatccg caaggcctgg ccggcgacgc
     1441 cattccgatc gcggcgcgcc tgatggcgat tgccgatgtc tacgacgccc tcataagccg
     1501 gcgtgtctac aaggagccga tgccgcacga gcaggccatc gtgctcatcg aagagggacg
     1561 cggcaagcat ttcgatcccg atgtcgtcga tgccttcctg agcatccagg acgagtgccg
     1621 ggccattgcc gcgcgttacc tggacacgca tgcggatctc gatgcggaga tcgacaggct
     1681 ggagcacatc gcggagcgga tgtaaggcag tagaggacga ctaagagcga tagatcaggg
     1741 agcgctcgtg gacgcatctg ccgccgaacc cgccgaaccc gttgatgcgc cgcccgccga
     1801 gccggcggag tccgccgcag ctgagcctgg ccctgccgag cctgccttcg ccgaaccccc
     1861 gacgctggaa cctgccgcgg ccgatcttgc agcgcttgag cctgccgagg ccgcgccgcg
     1921 ccgcagccgc aaggcgctgg tgctcaagtc tgcgctcgct gtgttgcttg ccggtctcct
     1981 ggtgcaactc gtgagctggg agctgagcac ctcgacgctg ca
//
LOCUS       DASUCY010000570         2291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1410872, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000570 DASUCY010000000
VERSION     DASUCY010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410872"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VFV25_11215"
     CDS             <1..430
                     /locus_tag="VFV25_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HEY1393940.1"
                     /translation="TNIPRNDLVTTFLTGIAGLNQPRNVVASEMLRLNTAVPAVPFAQ
                     QNRLGIVGNILAGGNDNAGFPNGRRPKDDVVDISLVAVMGGLCVANGNNNALGFGVAC
                     NPSAVPLGKTSFNLNDGVDQAQLTLLPGFPYLNTPFGGTK"
     gene            441..686
                     /locus_tag="VFV25_11220"
     CDS             441..686
                     /locus_tag="VFV25_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393941.1"
                     /translation="MRIKLTLTAAAALLAGCGGGSDEALQPPQAAGDMVPSSATASVA
                     AYVQYVGSLPASEAAEPLRLDSVVAAPISDNAEPFAI"
     gene            748..1938
                     /locus_tag="VFV25_11225"
     CDS             748..1938
                     /locus_tag="VFV25_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393942.1"
                     /translation="MSLAPVLAALLGLLCLTTASAAPYVPASDAQIVETLRERPLDAT
                     DRAARRLRQEARRQPRNLALALAAARDAIGLARRDGDPRQLGLAQAALAPWWALAEPP
                     PAVLLLRATVLQSQHAFAAALADLDRLLADPATPLALRAQAELTRASLLQVQGRYAEA
                     AAGCERLASARYFSLGAGAAWIAEVCSAELRSLQGHARQAKTELVKLADRAVSPDQVA
                     WLALVRAELAGRMADDADAQALYRQALAASGRPDVYLLGSYADWLLDHGRASEAASLL
                     AGREAADALLLRLALAYQSARDPRAAGAIATLRERFEAAKLRGDALHRREEARMRLQL
                     LGEPQRALSLATENWGLQKEPADTRLLLEAAAAANQASAAQPVLRFIRETGYVDARLA
                     RWLP"
     gene            1935..>2291
                     /locus_tag="VFV25_11230"
     CDS             1935..>2291
                     /locus_tag="VFV25_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HEY1393943.1"
                     /translation="MTRRWLLRVMLPALLCAMAPAWAHKPSDSYLVLQAQGGRIELRW
                     DIALRDLDRELALDADDDGRLSWGEVRGRWSEIERYAFERVSLQVEGRSCAIAASGKP
                     QLDRHSDGSYVVLTRSL"
BASE COUNT          335 a          838 c          781 g          337 t
ORIGIN      
        1 gaccaacatc ccgcgcaatg acctagtcac gaccttcctc acaggcattg ctggcctcaa
       61 ccagcccagg aacgttgttg cctccgagat gctgaggctc aacaccgcgg tccccgccgt
      121 gcccttcgcg cagcagaacc ggctgggcat cgttggcaac atcctcgcgg gcggcaacga
      181 caatgccggc ttccccaacg gccgccggcc caaggacgat gtggtcgaca tctcgctggt
      241 cgcagtcatg ggcgggctgt gcgtggccaa cggcaacaac aacgcgctgg gcttcggcgt
      301 ggcctgcaac ccctcggccg tgccgctggg caagacgtca ttcaacctga acgacggcgt
      361 ggaccaggct cagctgacgc tgttgccggg cttcccttat ctcaacacgc ccttcggcgg
      421 cacgaagtga ggagcccatc atgagaatca agctcaccct cacggccgca gccgcgctgt
      481 tggctggctg cggcggcggc agcgatgaag cactgcagcc gccacaggcc gccggcgata
      541 tggtgccaag ctccgccacc gccagcgtcg cggcctatgt gcaatacgtg ggctcgctac
      601 cggccagcga ggcggccgag ccgctgcgcc tcgacagcgt cgtcgccgcg ccgatcagcg
      661 acaacgctga gccgttcgcc atctgagcaa gggcggccgg caacagccgg ccgctcttgc
      721 gcgcaggcca gcgcacccgc caccatcatg agtctcgctc ctgtccttgc agcgttgctt
      781 ggcctgttgt gcctcacaac ggccagcgcg gcgccctatg tgcccgccag cgatgcgcag
      841 atcgtggaga ccctgcgcga gcggccgctc gatgccactg atcgcgccgc acgtcgcttg
      901 cggcaggagg cacgccgcca gccgcgcaac ctcgcgctcg cgcttgctgc tgcacgcgac
      961 gccatcggtc tcgcgcgacg cgacggcgac ccgcgccagt tggggctggc ccaggctgcg
     1021 ctcgctccct ggtgggcact ggccgagccg ccgccagcgg tcctcctgct gcgcgccact
     1081 gtgctgcaga gccagcatgc cttcgcagcg gccctggcgg acttggaccg cctgctggcc
     1141 gaccccgcaa cgccgctcgc cctgcgggcc caagccgagc tgacccgcgc ttcgctgctg
     1201 caggttcaag gtcgctatgc cgaggcggcg gccggttgcg agcgcctggc gagcgcacgc
     1261 tatttctcgc tgggcgctgg cgcggcctgg atcgccgagg tctgcagcgc ggagctgcgc
     1321 agcctgcaag gccacgcgcg ccaggccaag actgagctgg tcaagcttgc cgaccgcgcg
     1381 gtctcgcccg accaggtggc ttggctggcc ctggtgcgcg ccgagctggc cgggcgcatg
     1441 gccgatgacg ccgatgccca ggctctgtac cgccaggcgc tggccgccag cgggcgcccc
     1501 gatgtctacc tgctcggcag ctatgccgac tggctgctcg accatggccg cgcgagtgag
     1561 gcggccagtc tgctggccgg gcgcgaggcc gccgatgcct tgctgctgcg actggccctg
     1621 gcttatcaaa gcgcgcgcga cccgcgcgcc gccggcgcga tcgccacatt gcgcgaacgc
     1681 ttcgaggcgg caaagctgcg cggcgatgcg ctgcatcggc gcgaagaggc gcgcatgcgg
     1741 ctgcaactgc tgggcgagcc gcagcgcgcc ctgagcctcg ccaccgagaa ctgggggctc
     1801 cagaaagagc cggccgatac gcgcctgctg ctcgaagctg ctgccgctgc aaaccaagcg
     1861 tcggcagccc agccggtgct gcgcttcata cgcgagacgg gctatgtcga cgcgcggctg
     1921 gcgaggtggt tgccatgacg cgccgctggc tcctccgggt gatgctgccc gcgctgcttt
     1981 gtgcgatggc gcccgcctgg gcgcacaagc ccagcgacag ctacctcgtg ctgcaggcgc
     2041 agggcggccg catcgagctg cgctgggaca tcgcgctgcg cgacctcgac cgcgagctcg
     2101 cgctcgacgc cgacgacgat ggtcggctca gctggggcga ggtgcgcggc cgctggagcg
     2161 agatcgagcg ctatgccttc gagcgagtga gcctgcaggt cgagggccga tcctgcgcta
     2221 tcgccgcctc gggcaaaccg cagctcgatc gccacagcga cggcagctat gtggtgctga
     2281 cacgcagcct c
//
LOCUS       DASUCY010000571         3734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_318527, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000571 DASUCY010000000
VERSION     DASUCY010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318527"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VFV25_11235"
     CDS             complement(<1..348)
                     /locus_tag="VFV25_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEY1393944.1"
                     /translation="MFTGIVQGIATVVAVTDRPGLRSFRLQFPAGFAADLAIGASVAV
                     DGVCLTVTALHGEQAADFDVMQQSLALTTLGTLAVGSRINVERAAKDGAEIGGHPLSG
                     HVDFQARLAAIRKP"
     gene            495..947
                     /locus_tag="VFV25_11240"
     CDS             495..947
                     /locus_tag="VFV25_11240"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEY1393945.1"
                     /translation="MSIHFEHSIDVEQSPERVFAVLDDLSQTPKWLARCTGIQILTPG
                     PLAVGSKLRYGYREGGRSGEMDGELVARDPGERLGYKYWDKMMEVSVNFRMGSSPQPG
                     AVTRLVHTIEIAPKTFLAKLFSPMIRRQLPKQTITAMESLRALLKQQT"
     gene            complement(944..1549)
                     /locus_tag="VFV25_11245"
     CDS             complement(944..1549)
                     /locus_tag="VFV25_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HEY1393946.1"
                     /translation="MEAATHLLYLHGFRSSPQSNKARRMAVQVESLRARGRDLSWCCP
                     QLPPSPAAAMQLIRERVADWPKERIAVIGSSLGGFYATVLAEEITSCRAVLLNPAVDP
                     ARDLAGYIGEQTAWHDPSEHFYFRAEYVDELKALTPTAITRPERYFAIIAKGDELLDW
                     REMMARYAGCRIKLLEGGDHALSDFDTGHLPAVIAFLGLGA"
     gene            complement(1556..2908)
                     /gene="mpl"
                     /locus_tag="VFV25_11250"
     CDS             complement(1556..2908)
                     /gene="mpl"
                     /locus_tag="VFV25_11250"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEY1393947.1"
                     /translation="MHIHILGICGTFMGGLAALAREAGHRVTGCDAGVYPPMSDQLRA
                     LDIELIEGYKADQLAIRPDLYVIGNVVTRGNELMEAILDAGLPYTSGPQWLAEHVLQG
                     RHVLAVAGTHGKTTTTAMLAWVLEHAGLQPGFLVGGVPQNFGVSARLGQGKCFVIEAD
                     EYDTAFFDKRSKFVHYHARTAVLNNLEYDHADIFPDLAAIETQFHHLVRTVPHAGRLV
                     VNAREEILDRVLQRGCWSEVARFGTRGDAPGWRARGEPHAFDVLKSGVLAARVEWDLL
                     GEHNQMNALAAIAAAEHVGITPAAAAKGLSEFRNVRRRLELRGEAGGVKVYDDFAHHP
                     TAMRTTVDGLRRKVGLERILAVFEPRSNTMKLGTMKAQLPWALEEADLAFCHSGGLGW
                     DAAESLAPMGDKVVVSDKIDTLVQRIVKAARPGDHILCMSNGGFGGIHLKLLEALGHR
                     "
     gene            3018..>3734
                     /locus_tag="VFV25_11255"
     CDS             3018..>3734
                     /locus_tag="VFV25_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M3"
                     /protein_id="HEY1393948.1"
                     /translation="MTTTMTTADNPLLQPWNTLYGLPPFDRITPAHFAPAFEQALAAH
                     LAEIDAIAASPQAPGFDNTVAALDRSGRALRRIEGVFHNLCASESTPALRAVEREMAP
                     KLAAHESAIYLNARLFARLEALHEQRELLGLAPEQQRLLERIHLDFVMAGARLAPEAQ
                     ARYAQVMQRLAELNTQFGQHVLGDEAAWQLALNGEADLAGLPEWVRNAAREVATQRGS
                     DGHMVSLSRSLVMPFLTFSRR"
BASE COUNT          588 a         1268 c         1273 g          605 t
ORIGIN      
        1 gggcttgcga atggcagcga gccttgcctg aaagtccaca tggcccgaca gcgggtgacc
       61 gccgatctcg gcgccgtcct tcgcggcgcg ctcgacgttg atgcggctgc ccacggccag
      121 cgtgcccagc gtcgtgagcg caaggctttg ctgcatcacg tcgaagtcgg cggcctgctc
      181 gccatgcagc gcggtgaccg tgaggcacac gccgtcgacg gcgacgctgg cgccgatcgc
      241 gagatcagcg gcgaagcctg caggaaattg cagcctgaag ctgcgcagcc cgggccggtc
      301 ggtgacggcg accacggtgg cgatgccttg gacgatgcct gtgaacatgc tgaggagatc
      361 tggaatagag gagttttgga gcttgacggg gagcttagca gtcggcgaaa aatgtcgcgt
      421 cttcaggctt gcctcgttta gcatgcggcc ccattgcccc attgcagtag tcggctgcga
      481 agcaggagat ggccatgtcc attcatttcg aacacagcat cgatgtcgag cagagcccag
      541 agcgggtttt tgcggtgctc gacgatctct cgcagacgcc caagtggctg gcccgctgca
      601 ccggcatcca gatcctcacg ccggggcctc tcgccgtggg cagcaagctg cgctacggct
      661 atcgcgaggg cggccgcagc ggcgagatgg acggcgagct cgtggcgcgc gaccccggcg
      721 agcggctggg ctacaagtac tgggacaaga tgatggaggt ctcggtgaac ttccgcatgg
      781 gctcgagccc gcagcccggc gcggtcaccc ggctggtgca caccatcgag atcgcgccca
      841 agaccttcct cgccaagctc ttctcgccga tgatccgccg gcaactgccc aagcagacca
      901 tcacggcgat ggagagtctg cgcgccttgc tgaagcagca gacctaggcg cctaagccca
      961 ggaaggcgat tacggccggc aggtggcccg tgtcgaagtc gctcaaggcg tggtcgccgc
     1021 cttcgagcag cttgatgcgg cagccggcat aacgcgccat catctcgcgc cagtccagca
     1081 gctcgtcgcc cttggcgatg atcgcgaaat agcgttcggg ccgcgtgatc gcggtgggcg
     1141 tgagcgcctt cagctcgtcg acgtattcgg cacggaagta gaagtgctcg ctagggtcgt
     1201 gccaagcggt ctgctcaccg atgtagcctg cgagatcgcg cgccgggtcg accgccgggt
     1261 tcagcagcac ggcgcggcaa ctggtgattt cctcggccag caccgtcgca tagaagccgc
     1321 ccagcgagct gccgatcacg gctatgcgct cctttggcca gtccgcgacg cgttcgcgga
     1381 tcagctgcat ggccgcggcg ggcgagggcg gcagctgagg gcagcaccag ctgaggtccc
     1441 ggccccgcgc gcgcaggctt tccacctgca ccgccatgcg gcgcgccttg ttcgattgcg
     1501 gcgacgagcg gaagccgtgc aggtagagca gatgggtcgc tgcttccatc tcgggttagc
     1561 ggtggcccag agcttcgagc aacttgaggt ggataccgcc gaagcccccg ttgctcatgc
     1621 acaggatgtg gtcgcccggc cgcgccgcct tgacgatgcg ttgcaccagc gtgtcaatct
     1681 tgtcgctcac gaccaccttg tcgcccatcg gcgccagcga ttcggcggcg tcccagccga
     1741 gcccgccgct gtggcagaag gcgaggtcgg cctcttcgag cgcccagggc agctgggcct
     1801 tcatcgtgcc cagcttcatc gtgttggagc gcggctcgaa gacggccagg atgcgctcca
     1861 gcccgacctt gcggcgcagg ccgtcgaccg tggtgcgcat cgcggtcggg tggtgggcga
     1921 agtcgtcgta gaccttgacg ccgcccgcct cgccgcgcag ctccaggcgg cgcctgacat
     1981 tgcggaactc gctcaagccc ttggccgccg cggccggcgt gatgcccaca tgctcggccg
     2041 cggcgatggc ggccagcgca ttcatctggt tgtgctcgcc cagcagatcc cactccaccc
     2101 gggccgccag cacgccggac ttcagcacgt cgaaggcgtg gggttcgccg cgggcgcgcc
     2161 agcccggcgc gtcgccgcgc gtgccgaagc gcgccacctc gctccagcag ccgcgctgca
     2221 gcacgcggtc caggatttcc tcgcgcgcgt tgaccaccag gcggccggcg tgcggcacgg
     2281 tgcgcacgag gtgatggaac tgggtctcga tcgcggccag gtcggggaag atgtcggcgt
     2341 ggtcgtattc gaggttgttg agcaccgcgg tgcgcgcgtg gtagtgcacg aacttgctgc
     2401 gcttgtcgaa gaaggccgtg tcgtattcat cggcctcgat gacgaagcat ttgccttggc
     2461 ccagccgtgc cgaaacgccg aagttctgcg gcacgccgcc caccaggaag cccggctgca
     2521 gtcccgcatg ctccagcacc caggccagca tcgccgtggt ggtggtcttg ccatgcgtgc
     2581 cggccacggc cagcacgtgg cggccttgca gcacatgctc ggccagccat tgcgggccgc
     2641 tcgtataggg caggcccgcg tcgaggatgg cctccatcag ctcgttgccg cgcgtcacga
     2701 cattgccgat cacgtagagg tcgggccgga tcgcgagctg gtcggccttg tagccctcga
     2761 tgagttcgat gtcgagcgcg cgcagctgat cgctcatcgg cggatagacg cctgcatcgc
     2821 agccggtgac gcgatgtccg gcttcgcggg ccagcgcggc gaggcccccc atgaaggtgc
     2881 cgcagatgcc aaggatgtgg atgtgcatcg cgcgattgta gaaagccgct ccgtaacgat
     2941 cggctacctg caggcggcta ccatgcgcct tccgccctac cgggcgccca tatcagccac
     3001 cggctcaaga ggccggcatg acgacgacga tgacgaccgc cgacaacccg ctgctgcagc
     3061 cctggaacac gctctacggc ctgcctccct tcgaccgcat cacccccgcg catttcgcgc
     3121 cggccttcga gcaagcgctg gccgcgcacc tggccgagat cgatgccatt gccgcctcgc
     3181 cgcaggcgcc tggcttcgac aacacagtgg cggcgctcga ccgcagcggc cgcgcgctgc
     3241 gccgcatcga aggcgtgttc cacaacctct gcgcatccga gagcacgccg gccttgcgtg
     3301 ccgtggagcg cgagatggca cccaagctgg ccgcgcatga aagcgcgatc tacctcaatg
     3361 cccggctgtt cgcgcgcctc gaggcgctgc acgaacagcg cgagctgctc ggcctcgcgc
     3421 ccgagcagca gcgcctgctg gagcgcatac atctggactt cgtgatggcc ggcgcgcgcc
     3481 tcgcgcccga ggcccaggcg cgctatgcgc aggtgatgca gcggctggcc gagctcaaca
     3541 cgcaattcgg ccagcatgtg ctgggcgacg aggccgcctg gcagctggcg ctgaacggtg
     3601 aagccgatct ggcaggcctg cccgagtggg tgcgcaatgc ggcgcgcgag gtggccacgc
     3661 agcgcggcag cgacggccac atggtgtcgc tgtcgcgctc gctcgtgatg cctttcctca
     3721 cctttagccg gcgg
//
LOCUS       DASUCY010000572         8968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1089557, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000572 DASUCY010000000
VERSION     DASUCY010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 8968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8968
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1089557"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VFV25_11260"
     CDS             <1..343
                     /locus_tag="VFV25_11260"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEY1393949.1"
                     /translation="RALEMAWLMAEAGLVVITSLISPFRAERGAARVRFGAGRFIEVF
                     VDAPLSVCEARDPKGLYRRARHGEVRGLTGIDSPYEVPLDPELRVDTTRLSPEQGAAA
                     IAECLQARSMW"
     gene            complement(515..838)
                     /locus_tag="VFV25_11265"
     CDS             complement(515..838)
                     /locus_tag="VFV25_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393950.1"
                     /translation="MDAVVLLNKWLATQCRLLGDGLVQLAGSPLAMMTPWLGLQGELT
                     QQFLTLAAQTMQQMREADRDDKATDANAWAASSAALSQPWQRTLGDWWPQWSAFWMRG
                     MEQLA"
     gene            1064..4099
                     /locus_tag="VFV25_11270"
     CDS             1064..4099
                     /locus_tag="VFV25_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEY1393951.1"
                     /translation="MEHFTQLLASPGFMPHGHCFLWTPTLLWAYVLSDSLIALAYYSI
                     PVALWYFVRRRSDLPFGWVFIMFAVFIFACGTTHLMAIWNIWQPVYWLDAAIKAATAT
                     ASVATATLLWPLMPRALALPSQRQLTRVNEELRQQVLARRLAEERLQELNEALERRVA
                     ERTAALEAANMELRALAAERERASRSLQESQQLLQAIVDNVTAIVYVKRLDGRYLLVN
                     RQYQQLFHVTQQEMSAKTDFDLFDAEHAQAFRSVDEEVAASDRAIETEEVAPLDDGPH
                     VYLSIKFPVRDELGRPYAVAGISTDITERKRNEQALRENEERTRAILDAALDAVITMD
                     AQGTITGWNPQAEATFGWPREEVLGLALGETIIPPQHRSAHANGLRRYLSTGHAVVLR
                     KRLELTALHRDGHEFPVELTITPIGSGAQVRFSGFARDISDRKRSEAALKAQIERLNL
                     LDRITRAIGERQDLHSIFQVVVRALEDQLPLDFVCMCRHEDGAKTLEVTAIGARSQPL
                     AQQLTMTEQSSIVVDENGLSQCIRGRLVYEPDLHSVPQPFAQRLARAGLRSLVAVPLM
                     AQGQAFSALIASRHQTDGFSSGECEFLRQLCEHVALAAQQAQLYAALQEAYQELRQTQ
                     QAVLQQERLRALGQMASGIAHDINNALSPVTLYTESLLDQEAGLSERARGYLMTIEQA
                     VADISATVARMREFYREREPQLSLVPLLLNQLVQQVVELTRARWSDMAQQRGVVIEVH
                     TELAESLPYVSGMESEIREALINLIFNAVDAMPQGGRLTLRTGLVVPPPGSAPVPTKV
                     KVQVVDDGMGMDEETRRRCLEPFFTTKGERGTGLGLAMVYGMAQRHGADIDIASAVSQ
                     GTTVSLTFPSTANGEAAAPGSAKQPPVPAGLRLLVVDDDPVVLQSLGDVLRGDGHVVV
                     AVSGGQAGIDAFTDAFHQGGAFSAVITDLGMPYVDGRRVASAVKRIAPSTPVLLITGW
                     GQRMIAEGDIPEHVDRVLSKPARLGELREALALCRPVED"
     gene            4129..5262
                     /locus_tag="VFV25_11275"
     CDS             4129..5262
                     /locus_tag="VFV25_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY1393952.1"
                     /translation="MSTDAPRLLVVDDEPAQMAALCNTLRDQGYEPLGFTSARDALAS
                     LQERPCELLLTDLRMSEIDGIALLRAALEIDPQIVGIVMTGEGTIASAVEAMRAGAFD
                     YILKPFRLSGILPVLQRALAVRQLRVDNARLQRYVEERTAELEVSNRELAAANKALAA
                     FSSSVSHDLRAPLRAISGFTQILLEEHAAALPEEALQLLHKVSAGGERMHSLIDGLLR
                     LAQLGRQPLEKRPVQLAALAREVLRDLGGQLAGRDIDVRIAELPACVADAALMRQVLV
                     NLLSNALKFTRHHQPAVVELGSVTQRGDTVYFVRDNGAGFDMVHAERLFEAFTRLHRP
                     AEFEGTGIGLSIVQRIIERHGGRIWADAQPGQGATFYFALPPD"
     gene            5593..6825
                     /locus_tag="VFV25_11280"
     CDS             5593..6825
                     /locus_tag="VFV25_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY1393953.1"
                     /translation="MPKTFAMTLVPLAGALLLATAAGKAAGTEGDTIVLGAAVSLTGK
                     YSVNGKNTKDGYDLAVKRINERGGVKVGGRTYLIKVVYYDDESTPARGSQLAERLIVQ
                     DGVKFVLGAYSSGLTKAIAPVTEKYKIPHVEGNGAARELFTEGYRYLFAVLSTSDYYL
                     RDAVNLAAEVARQHGRDPRTLKIAMAFENDPFSLDVRDGVMEDAARHGMRVVIDDKLP
                     PELNDMAATLTKVRAVKPDLLIVSGHEKGATLVVRQTAEMKVDVPMIATTHCDSAQIA
                     ENLGALAESLLCASQWDRTLSYQDKWFGTAEQYAQLFKKEFAYEAPYQAAESTASVLV
                     FADAFERAQSLQPEKVRDAIASTDMMTFYGPIKFDSTGKNTAKPMVLYQVQGGHYRVV
                     APTKWAEAKLVWPRKAAK"
     gene            6881..7780
                     /locus_tag="VFV25_11285"
     CDS             6881..7780
                     /locus_tag="VFV25_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY1393954.1"
                     /translation="MDNLRLLFDAPALSVQLLFDGLLIGAIFALPAYGMALVWGVMDL
                     VNIAQGEFVMLGGYVALFLYTAGIHPLLGVPLAALLMFVLGWGLYRGIIFRLVDKDRF
                     ISILATFGLSISLQQLANALFGANVRTAESGFGTLVLFGGSVTVAEIKLVSFAAALAV
                     GAVLVWFLKRSRMGRAIRATAQNARAARIMGVDTDRVYAWAFGINAAICGAAGALVAM
                     TWVVHPYLGLPYTVRSFMIVVVAGLGNVVAVLVAALGLGVAENFAGFILGAEYQTAFV
                     FGFLVAILVWRRFSLGRQRRVLR"
     gene            7777..8766
                     /locus_tag="VFV25_11290"
     CDS             7777..8766
                     /locus_tag="VFV25_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY1393955.1"
                     /translation="MKPVLPRRLYWLGDLILVAAALLLPMLLPGYRTQLAFLWVMVLF
                     ALTWDLQGGQMGYNSFGNILFFGIGMYACAVVQRDSGLGYFTALWLGMGAGALLAVAV
                     AACLGSAMLGLRGHYFAIGTLGLGIAAGEAAGGWQFIGAGAGLVPPLFPGALGAREGF
                     FYYLCFALAAITFALLRWLYAGRFGLAINAIRDDEDKAEAMGLRTTQYKTTAWCISAF
                     FLALAGGAVGNMTGFIDPREVAFASTTFGVWMVLMAILGGKGTLWGPIVGAVIFHVTK
                     ELFWTYLLGWQRVALGLLIMIIVVFFPRGMLGWAHERWPGLSGQRAEGLEPRR"
     gene            8763..>8968
                     /locus_tag="VFV25_11295"
     CDS             8763..>8968
                     /locus_tag="VFV25_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY1393956.1"
                     /translation="MTTILSVRRLRKTFGGVVAVNDVSLDVSEGQIVGLIGPNGSGKT
                     TLFNSIVGHHPIDAGSVRFQGRELS"
BASE COUNT         1426 a         2865 c         3077 g         1600 t
ORIGIN      
        1 tcgcgccctg gagatggcct ggctgatggc ggaggcgggt ctggtggtga tcacgtcgct
       61 gatatccccg ttccgcgctg aacgcggcgc cgcccgcgtg cgttttggtg cgggacgctt
      121 catcgaggtg ttcgtcgacg cccccctgtc ggtctgtgag gcacgggatc ccaaaggttt
      181 gtaccggcgg gcgcgccacg gcgaggtgcg cgggctgacc ggcatcgatt cgccttacga
      241 agtgcccctt gatcccgagc tgagagttga cacgacgcgg ctcagccccg agcagggtgc
      301 cgcagccatc gccgagtgcc tgcaggcgcg ctccatgtgg tgatcgacgt ggcaacgctg
      361 cggctactgg gcaacgagac ccaagtgctg tcgcacagcg aggcgtctgt gctggtatcg
      421 agatcgaacc aagctgagca ccgacgaggc attcacggcc gaggctgcgg accctgggtt
      481 cgcacggtgc gcagcaactc gtccgaggcg tgcgtcaggc gagttgctcc atgcctcgca
      541 tccagaaagc tgaccattgc ggccaccagt cgccgagggt gcgttgccag ggctggctca
      601 gcgctgcgga ggaagctgcc cacgcattgg cgtcggtggc tttgtcatcg cgatctgcct
      661 ctcgcatttg ctgcatcgtc tgcgcggcca acgtcaggaa ctgctgcgtc aattcgccct
      721 gaagccccag ccacggagtc atcatcgcca gtgggcttcc agcgagctgg acaaggccgt
      781 cgccgagcag gcggcactgc gtcgctagcc atttgttcag caagaccacg gcatccaccg
      841 cggcgtcgtc cccagacgtg agcacagaac cgccgggaac tgacttacga gccataacgc
      901 cccctcatca ggtacgagcc ttcgacactc ttgtgcccag gggttggtat gtttgcatct
      961 gtcgcgtggc gatgcaagcc attcaccgac gggcggcgct tgaggatcat cggcttcagc
     1021 gtgatactgc gcacgaccat ggacgccagg gaggaactgc accatggaac acttcaccca
     1081 gctgctggcc tcgcccggct ttatgccgca cgggcattgt ttcctctgga cgccgacgct
     1141 gctgtgggcc tatgtgctgt ccgactcgtt gattgcgctg gcctattact cgatcccggt
     1201 ggcgctgtgg tacttcgtcc ggcgccggtc ggatctgccg ttcggctggg tcttcatcat
     1261 gttcgcggtg ttcatcttcg cctgcggcac gacccatttg atggccatct ggaacatctg
     1321 gcagccggtg tactggctgg atgctgccat caaggcggcc actgcgacgg cctcggtcgc
     1381 caccgccacc ctgctttggc ctctgatgcc gcgggcactg gcgctgccca gccagaggca
     1441 gctgacccgt gtgaacgagg agctgcggca gcaggtcctg gcgcggcgcc tcgccgaaga
     1501 gcgattgcag gagctcaacg aagcgctgga acggcgcgtg gccgagcgca cagccgcgct
     1561 cgaggcagcc aacatggagc tgcgcgccct cgcggccgag cgcgagcggg cgagccgttc
     1621 gctgcaggaa agccagcagc tgctgcaggc gatcgtcgac aacgtcacgg ccatcgtcta
     1681 tgtcaagcgc ctggacggcc gctacttgct ggtcaaccgc cagtaccagc agctgttcca
     1741 tgtgacgcag caggagatgt ccgcaaagac tgacttcgac ttgttcgatg cggaacatgc
     1801 acaggccttc cgttcggtgg acgaggaagt ggccgccagc gaccgggcca tcgagaccga
     1861 agaagtggct ccgctggacg acgggccgca cgtctacctg tcgatcaagt tccccgtgcg
     1921 cgacgaactg ggacggccct acgccgtcgc gggcatctcg accgacatta ccgagcgcaa
     1981 gcgcaacgag caggccttgc gcgagaacga ggagcgcaca cgcgcgattc tcgacgccgc
     2041 gctggacgcg gtgatcacga tggatgcgca agggaccata accggctgga atccgcaggc
     2101 cgaggcgact ttcggctggc cgagagagga ggtgctcggc ctcgccttgg gtgaaacgat
     2161 catcccgcca cagcatcggt ccgcccacgc gaacggcctg cggcgctatc tcagcaccgg
     2221 ccacgccgtg gtcctgcgca agcggctgga gctcacggcc ttgcaccgcg acggccacga
     2281 gttccccgtc gagctgacca tcacgccgat cggcagcggc gcgcaggtgc gcttcagcgg
     2341 cttcgcgcgc gacatctcgg atcgcaagcg ttccgaggcc gcactgaagg cccagatcga
     2401 gcggctgaat ctgctcgacc gcatcacccg cgcgatcggc gaacgacagg atctgcatag
     2461 catcttccag gtggtggtgc gtgccctcga ggaccaactg ccgctcgact tcgtgtgcat
     2521 gtgccggcac gaggacggcg ccaaaacgct cgaagtcacc gcgatcggcg cccgcagcca
     2581 gccgctcgcg cagcagctga cgatgaccga gcagtcctcg atcgtggtcg acgagaacgg
     2641 cctttctcag tgcatacgtg gccgcctcgt ctacgagccg gacctccact cggtgccgca
     2701 gccattcgca cagcgcctgg cgcgcgccgg ccttcgatcg ctggtcgcgg tgccgctgat
     2761 ggctcaggga caggctttca gcgcgctcat cgcctccagg caccagaccg acggcttcag
     2821 cagcggcgag tgcgagttcc tgcggcagct atgcgagcac gtggcgctgg cggcccagca
     2881 ggcacagctg tacgccgcct tgcaggaggc ttatcaggaa ttgcgccaga cccagcaggc
     2941 cgtgctgcag caggagcggc tgcgcgcgct ggggcagatg gccagcggta tcgcccacga
     3001 catcaacaac gcgctgtcgc cagtgacgct gtacaccgag tcgctgctgg accaggaagc
     3061 cggtctgagc gagcgtgcgc gcggctactt gatgactatc gagcaggccg tcgccgatat
     3121 cagcgccact gtggcgagga tgagggagtt ctaccgcgag cgcgagccgc agctgagcct
     3181 cgtgccgcta ctgctgaacc agctggtgca gcaggtcgtc gagctgacgc gtgcgcgctg
     3241 gagcgacatg gcacaacagc gcggcgtcgt gatcgaagtt cacaccgagc tggcggaatc
     3301 cctgccatat gtgtcgggca tggagagcga aatccgcgag gccttgatca acctcatttt
     3361 caacgcggtc gatgccatgc cgcagggagg ccgactgacg ctgaggaccg gcttggttgt
     3421 gccgcccccg gggagcgcgc cggtcccgac gaaggtcaag gtgcaggtgg ttgatgacgg
     3481 catgggcatg gatgaggaaa cgcgccgtcg ctgcctggag ccgttcttca cgaccaaggg
     3541 cgagcgcggc accggcttgg gcttggccat ggtctatggc atggcgcagc ggcatggggc
     3601 cgacatcgac atcgccagcg cagtcagtca ggggacgacg gtctccctga ccttcccctc
     3661 gactgccaac ggggaagcgg ccgcgccggg ctccgccaag cagccgccgg tgccggcggg
     3721 tttgcgcctg ctggtggttg acgacgatcc ggtggtgctg cagtcgttgg gcgacgtgct
     3781 gcgcggcgac ggccatgtgg tggtagccgt cagtggcggg caggccggca tcgacgcctt
     3841 cacggacgcc ttccatcagg gaggcgcctt ctcggccgtc atcacagacc tgggtatgcc
     3901 ctatgtggac ggccgccggg tagccagcgc ggtcaaacgg atcgcgccat cgacgccagt
     3961 gctgctgatc accggctggg ggcagcgcat gattgccgaa ggcgacatcc ccgagcatgt
     4021 ggaccgggtg ctgagcaagc cggccaggct cggcgagctg cgcgaggcgt tggcgctgtg
     4081 ccgcccggtc gaggactgat cgatacctgc cgtcagaggg agtggtccat gtcaaccgat
     4141 gcccccaggc ttctcgtcgt ggacgacgag cctgcccaga tggcagcgct gtgcaacacg
     4201 ctgcgcgacc agggctatga gccactcggc tttacgtccg cgcgggacgc gctggcgtca
     4261 ctgcaagagc gcccctgcga gctgctgctg accgacctgc ggatgtccga gatagacggc
     4321 atcgcgctgc tgcgtgccgc gctggagatc gatccgcaga tcgtgggcat cgtgatgacc
     4381 ggcgaaggca ctatcgcttc cgcggtggag gccatgcggg ccggggcctt cgactacatc
     4441 ttgaagccct tcaggctcag cggcatccta cccgtgctgc agcgcgcact ggccgtgcgc
     4501 cagctgcggg tcgacaatgc tcgcctgcag cgttacgtcg aagagcgcac ggccgagctg
     4561 gaggtcagca atcgcgagct ggcggcggcc aacaaggcgc tggccgcctt ctcgtcgtcg
     4621 gtgtcccatg acctgcgcgc cccgctgcgc gccatcagcg ggttcacgca gatcctgctg
     4681 gaggagcacg cggcggcgct gccggaggaa gcgctgcagc tcctgcacaa ggttagcgca
     4741 ggcggcgagc gcatgcactc gctgatcgat ggcctgcttc gcctggcgca gttgggtcgc
     4801 cagccactcg aaaagcggcc ggtacagctc gcggcgctgg cgcgcgaggt tcttcgcgac
     4861 ctgggcgggc aactggcggg ccgcgacatc gatgtccgca tcgccgagct gccggcctgc
     4921 gtagccgatg cggcgctgat gcggcaggtg ctggtcaacc tgctgtccaa tgcgctgaag
     4981 ttcacgcggc accatcagcc agccgttgtc gagttgggct cggtgaccca gcgtggcgac
     5041 acggtgtact tcgtccgcga caacggcgcg ggcttcgaca tggtgcatgc cgagaggctg
     5101 tttgaggcgt tcacccgcct gcatcgcccg gccgagttcg aaggcaccgg catcggactt
     5161 tccatcgtcc agcggatcat cgagcgccat ggcgggcgca tctgggccga tgcccagcca
     5221 ggacagggcg cgacgttcta cttcgcgctg ccgcccgact aagagtcgtt ggtttcaacg
     5281 aggaagggta gcgcgagcct cagcgccacc ttcacgagct ccttagtgcg cctaatgaac
     5341 cgcagccgcc gcgactgctt caggagcacc ctgcggggat gcctgggagc tctcgcgttg
     5401 aaggtctgct tctacgagga ctgccttgtc aaggcatgac gacggtgttt acggcggcgc
     5461 gcgttcgcca cgcgggcaga agtgagatca ggtgagagca gggttggtca atgggctcca
     5521 tctttctggt gaaccgcttg tcagtgccgg tgccaagggt catagtagct tcggacgcac
     5581 aaggagagag tcatgccgaa gacgttcgca atgacgctcg tgcctctcgc aggcgcgctg
     5641 ctgctggcga cggcggccgg caaagccgcc gggaccgaag gcgacaccat cgttctcggc
     5701 gcggccgtgt cgctcaccgg caagtattcg gtcaacggca agaacacgaa ggacggctac
     5761 gatctcgcgg tcaagcgcat caacgaaaga ggcggggtca aggttggcgg caggacctat
     5821 ctgatcaagg tcgtttacta cgacgacgag tccacgcccg cgcgcggatc gcagctcgcc
     5881 gagcgcctga tcgtgcagga cggcgtgaag ttcgtgctcg gcgcctacag ctcggggctc
     5941 accaaggcga tcgcgcctgt caccgagaag tacaagattc cgcatgtcga aggcaatggc
     6001 gccgcgcgcg agctcttcac cgagggctac cgctacctct ttgccgtgct gtccacctcg
     6061 gactactacc tgcgcgatgc ggtgaacctg gccgccgaag tcgccaggca gcatggccgc
     6121 gacccaagga cgctcaagat cgcgatggcg ttcgagaacg atccgttcag cctggacgtg
     6181 cgcgacggcg tgatggagga tgctgcgcga cacggcatga gggtggtcat cgacgacaag
     6241 ctgccgcccg agctgaacga catggcggcc acactcacca aggtgcgggc cgtgaagccg
     6301 gacctgctga tcgtgtccgg gcatgagaag ggggcgactc ttgtagtgcg gcagacggcg
     6361 gagatgaagg tggatgtgcc gatgatcgcc accacgcact gcgactccgc acagatcgcg
     6421 gagaacctgg gcgcgctcgc cgagtctctg ttgtgcgcgt cgcagtggga ccgcaccctg
     6481 agctatcagg acaagtggtt cggcactgcc gagcagtatg cgcagctctt caagaaggag
     6541 ttcgcctacg aggcgcccta tcaggcggcg gagtcgacgg cttccgtgct ggtctttgcg
     6601 gatgcgttcg agcgtgcgca gtcgctccag cccgagaagg tgcgcgatgc catcgccagc
     6661 accgacatga tgaccttcta cgggccgatc aagttcgact cgacgggcaa gaacaccgcc
     6721 aagcccatgg ttctctacca ggtgcaaggt gggcactaca gggtggtggc tccgacgaaa
     6781 tgggccgagg ccaagctggt ctggccgcgc aaggccgcga agtagcggtc tcgtcgcggc
     6841 gcccgttccg gcggccgggg gccgaaggga gtagggcgag atggacaacc tgcggttgct
     6901 tttcgatgca cccgcgctgt cggtgcagtt gctgttcgac ggcctgctga tcggcgcgat
     6961 cttcgcgctg ccggcctacg gcatggcgct cgtctggggc gtgatggacc tggtcaacat
     7021 cgcgcagggc gagttcgtca tgctcggggg ctatgtcgcg ctcttcctgt acaccgccgg
     7081 gattcacccg ctgctgggcg tcccgctggc cgccctgctg atgttcgtgc tgggttgggg
     7141 gctgtaccgc ggcatcatct tccgcttggt ggacaaggac cgcttcattt ctatcctcgc
     7201 caccttcggc ctcagcattt cgctgcagca actcgccaat gcgctgttcg gcgcgaacgt
     7261 gcgcacggcc gagtccgggt tcggcacctt ggtcttgttc ggcggctcgg tcacggtcgc
     7321 agagatcaag ctggtgtctt tcgcggcggc gctggccgtc ggcgcggtcc tggtgtggtt
     7381 cctcaagcgc tcccgcatgg gccgcgcgat ccgtgccacg gcccagaacg cgcgggctgc
     7441 gcgcatcatg ggcgtggaca cggaccgggt ctatgcctgg gccttcggta tcaacgcggc
     7501 catctgcgga gcggccgggg ccttggtggc gatgacctgg gtggtgcatc cgtacctcgg
     7561 cctgccgtac acggtgcgct cgttcatgat cgtggtcgtc gccgggctcg gcaacgtggt
     7621 ggccgtgctc gtggccgcac tgggattggg cgtggcggag aacttcgcgg gcttcattct
     7681 tggtgcggaa taccagaccg cgttcgtctt cggcttcctc gtcgccattc tggtgtggcg
     7741 gcgcttctcg ctcggccgcc agcgcagagt cctgcgatga agcctgtctt gccgcgccgc
     7801 ttgtactggt tgggcgatct catcctcgtg gccgcggcgc tgctgctgcc tatgctcctg
     7861 cccggctacc gcacgcagct ggcgttcctg tgggtgatgg tgttgttcgc gctcacctgg
     7921 gacctgcagg gcggccagat gggctacaac tccttcggga acatactgtt cttcggcatc
     7981 ggtatgtatg cctgcgcggt cgtgcagcgc gactctggcc ttggctactt cactgcgctg
     8041 tggctgggta tgggggcggg ggccttgctc gccgtggccg tggccgcctg cctcggttcc
     8101 gcgatgctcg gcctgcgcgg ccactacttc gcgatcggaa cgctggggtt gggcatcgcc
     8161 gcgggcgagg cggccggtgg ctggcagttt atcggcgctg gtgcggggct ggtgccaccg
     8221 ctctttccgg gagcgctcgg cgcgcgcgag ggcttcttct actacctgtg cttcgcgctg
     8281 gcagccatca ccttcgcgct gttgcgctgg ctgtacgccg ggcgcttcgg actcgcgatc
     8341 aacgcgatcc gcgatgacga ggacaaggcc gaggccatgg gcctgcgcac cacgcagtac
     8401 aaaaccacgg cgtggtgcat ctcggccttc ttcctcgcac tggccggcgg cgccgtgggc
     8461 aacatgaccg gcttcatcga tccgcgcgag gtggcattcg ccagcacgac attcggcgtg
     8521 tggatggtgc tgatggcgat cctgggcggc aagggcacgc tatgggggcc gatcgttgga
     8581 gccgtgattt tccacgtgac caaggagctc ttctggacct acttgctcgg ctggcagcgc
     8641 gtggcgctgg gcttgctgat catgatcatc gtggtgttct tcccgcgcgg gatgctcggc
     8701 tgggcgcatg agcgctggcc gggtctgtcc gggcagcggg ccgaggggct ggagccgcgc
     8761 cgatgacgac aatcctcagc gtgcgccgtc tgcgcaagac tttcggcggc gtggtcgccg
     8821 tgaacgacgt cagcctggac gtgagcgagg gccagatcgt gggtttgatc ggccccaacg
     8881 gctcgggtaa gaccacgctg ttcaattcga tcgttgggca ccaccccatc gatgccggct
     8941 cggtgcgctt ccagggccgc gagctttc
//
LOCUS       DASUCY010000573         6386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1266306, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000573 DASUCY010000000
VERSION     DASUCY010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 6386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6386
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266306"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VFV25_11300"
     CDS             complement(<1..253)
                     /locus_tag="VFV25_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY1393957.1"
                     /translation="MKRRHLLLGAAAAGGALLVGWAVLPPRSRLGSQDLLSAAPGEIS
                     LNGWIKIVADGAVVLAMHRSEMGQGVHTALAQLVAEELDI"
     gene            complement(250..762)
                     /locus_tag="VFV25_11305"
     CDS             complement(250..762)
                     /locus_tag="VFV25_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY1393958.1"
                     /translation="MPSLTVNGQVHELPPGSTDPAMPLLWVLRDVLQLTGTKFGCGVA
                     ACGACTIHLGGQPVRSCVTPLSAAQGQSISTIEALADAAPLGPALQQAWVQHQVPQCG
                     YCQSGMLMAAAALLQHNLRPSDADIDAAMTNLCRCGSYPRVRAAIHAAAALLAGKPQG
                     GDASHTRAVA"
     gene            888..1868
                     /locus_tag="VFV25_11310"
     CDS             888..1868
                     /locus_tag="VFV25_11310"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEY1393959.1"
                     /translation="MKHFVRAGAALALAALACGARAQAANENNVYGLFDLAVGAVKPR
                     GGVSDKGVESGNMSTSYLGLRGVEDLGGGLSTVFALESFLRANNGAVGRYDGDTFWSR
                     NAYAGFSGAFGSTTLGRNTTPLYVSTLTFNAFGDSYGYSPSIRHYFSSGTVTGDSGWN
                     SSMLFTSPVGDNGLRISMAVAAHQGGEGRKFGGNLLYAKGPVGASFAWQAVKSGPTQN
                     GTGTWQLAGSYDFTLAKLYAQYGEVKNGDTGNKYKLFDLSASVPLGAGRLLGAYGHMS
                     PAVGNKLVTYSLGYDYDLSKRTDAYAAVMSDKQDTLSSGNAYSLGLRHRF"
     gene            complement(2014..3735)
                     /locus_tag="VFV25_11315"
     CDS             complement(2014..3735)
                     /locus_tag="VFV25_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HEY1393960.1"
                     /translation="MPTTPPPRPQPQFDRFYKYGELTELLQAYVAAHPQLLKLGSLGK
                     SHEGRDIWLLTITNQATGADTDKPAFWVDGNIHAAELTASTAVLYWLHQLVSGYGGDR
                     EITQLLDTRAVYLVPRLNPDGAELALSDTPRHIRSSTRPYPYDEDPIDGLTVQDMDGD
                     GRVLFMRVRDPHGSYKKHPTEPRLMVPREPGEFGGEYYRLMPEGRLENYDGLSLRVNP
                     DKEGLDLNRNFPSLWRQEHEQTGAGPYPTSEPEVRAMVDFIVRHPNIGAGVSFHTHSG
                     VILRPMGTEPDENMAPEDLWMYQRLSKLGEQLTGYPAVSIYHEFKYHPKEVITGTQDW
                     VYEHLGSLFWVVEIWAPNREAGVTDYKWIDWYRDHPVEDDLKLLKWSDEQCGGRAHVD
                     WYPFEHPQLGKVELGGWDKMNFWRNPPPHLREREAARFPKWLNTLALSLPRLELLSAR
                     AEHLGGSGGESRWRVTLDVGNAGYLPAYVSKRALAREQVRGLIFEIDLPPDASLVSGK
                     PRISEGVQLEGHAPKASLQAFLPTREITADRARAEWLVRSPAGAELTLSAKADRAGAI
                     RTRLKLG"
     gene            complement(3810..4568)
                     /locus_tag="VFV25_11320"
     CDS             complement(3810..4568)
                     /locus_tag="VFV25_11320"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEY1393961.1"
                     /translation="MTSTSVTALVADDEEAAREQLVEALQRCWPELQVVAEADNGVDA
                     WDAVLEHEPQICFLDVRMPGLTGIEVARRIDGCAHVVFVAAPGDRAVAAFESAAVDHV
                     IKPVDDAHLGHALEKLKRRLRHSPPADMHALLTQLLGQVRRPELPEVIQASLGREHRL
                     IRVDDVVYFESDARYTRVVLSDGDELIRMSLKELLPQLDTEQFWQIHRSVIVNHRHIA
                     GAQHQDDGNMVLMLVGRDEKLPVSRHFQGLFKAA"
     gene            complement(4783..5541)
                     /locus_tag="VFV25_11325"
     CDS             complement(4783..5541)
                     /locus_tag="VFV25_11325"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEY1393962.1"
                     /translation="MCQLLGMNCNTPTDVMFSFTGFASRSAEHKDGFGIAFFEHKGVR
                     LFVDDQAASASPVADMVKAYPIRSQNVIAHIRKATQGRVGLENCHPFVRELWGRYWVF
                     AHNGDLKGYSPRLHAAFHPVGDTDSEQAFCWLLQELAKNHATVPKVEALTQTLRELVP
                     GIAAHGNFNFMLSNGQALWAHCSTQLHWLVRQHPFRHAQLQDEDLTVDFAAVTTPSDR
                     VAVVVTEPLTRDEQWTAFAPGELRAFVNGAALAH"
     gene            complement(5553..>6386)
                     /locus_tag="VFV25_11330"
     CDS             complement(5553..>6386)
                     /locus_tag="VFV25_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase"
                     /protein_id="HEY1393963.1"
                     /translation="AIGAKLKLGGAVHEIHADWLILATGAVPQALIAAGLCTRRTPSG
                     VALRGYVRNEAMSDRLKALDVVWHRKLRPGYGWIFPCRDGVFNIGVGLAHSHSKFNDG
                     RRTMQDVNLRETFAAFCEFYEPARELTQGGTLLGELKGAPLRCSLEGAKYSRPGLIAT
                     GEAVGSTYAFTGEGIGKAMETGMLAAEAVLQGRHEKLDEVVVRQRYESELLALKPRFD
                     LYEKANRVNEHPWLADLLIWRAKRSERILRRMSGVLEETNNPGNLVTLKGLARLLLPI
                     S"
BASE COUNT          988 a         2123 c         2197 g         1078 t
ORIGIN      
        1 gaatgtccag ctcctcggcc accagttggg ccagcgcggt gtgcacgccc tggcccatct
       61 cgctgcgatg catcgccagc accacggcgc catctgcgac gatcttgatc cagccgttga
      121 ggctgatctc gcccggcgcg gcgctcagca agtcctggct gcccaggcgc gagcgcggcg
      181 gcagaacagc ccagcccacc agcagcgcac cgcccgctgc ggccgcgccc agtagtagat
      241 ggcgccgctt cacgccaccg cccgcgtgtg gctggcgtca ccgccttgcg gcttgccggc
      301 cagcagggcc gccgcagcat ggatggcggc gcgcacgcgc ggatagctgc cgcagcggca
      361 gaggttggtc atcgccgcgt cgatgtcggc atcgctgggc cttaagttgt gctgcagcag
      421 cgccgcggcg gccatcagca tgccgctctg gcaatagccg cattgaggca cctgatgctg
      481 gacccaggcc tgctgcagcg ccgggcctag gggcgcggcg tccgccaggg cttcgatggt
      541 ggagatgctc tgcccctgcg cggccgacag cggcgtgacg caggagcgca ccggctggcc
      601 gcccagatga atggtgcagg caccgcaggc ggccacgccg cagccgaact tggtaccggt
      661 gagctgcagt acgtcgcgca gtacccacaa cagcggcatc gccggatcgg tgctgcctgg
      721 aggcagttcg tggacttggc cgttgactgt caggctgggc atgggcgcta ccgtgcgggg
      781 gcggatgggc gattctgtac ccagacgtgt cgctcgggcg cgacgatttc cggcttcgag
      841 caaaatcgcg gcccatgcca tcgatcaatg aataaatgtc cgcacctatg aaacacttcg
      901 ttcgcgccgg ggccgcgctg gccctggccg ctcttgcctg cggcgcccgg gcgcaagcgg
      961 ccaatgaaaa caatgtttac ggcctgttcg atttagcagt gggtgccgtc aagccgcgcg
     1021 gcggcgtcag cgacaagggc gtggagagcg gcaacatgtc gaccagctac ctgggcctgc
     1081 gcggcgtcga agacctgggc ggcggcctga gcacggtgtt cgcgctggag agcttcctgc
     1141 gcgccaacaa tggcgctgtg ggccgctacg acggcgacac cttctggtcg cgtaacgcct
     1201 atgcaggctt cagcggcgcc ttcggcagca ccacgctggg ccgcaatacg acgccgctct
     1261 acgtttcgac gctgacgttc aatgccttcg gcgactccta tggctattcg cccagcattc
     1321 gccactactt cagcagcggc accgtcaccg gcgacagcgg ctggaacagc tccatgctct
     1381 tcacgtcgcc ggtcggcgac aacggcctgc gcatcagcat ggccgtggcc gcccaccagg
     1441 gcggcgaggg tcggaagttc ggcggcaatc tgctctatgc caaggggccg gtaggcgcca
     1501 gcttcgcctg gcaggccgtc aagtcggggc cgacccagaa cggcaccgga acctggcagc
     1561 tcgccggctc gtatgacttc acgctggcca agctctacgc ccagtacggc gaggtcaaga
     1621 acggcgacac cggcaacaag tacaagctgt tcgacctcag cgcctcggtc ccgctgggcg
     1681 ccggccggct gctcggcgcc tatggccaca tgagcccggc cgtcggcaac aagctcgtca
     1741 cctacagcct gggctacgac tacgacctgt ccaagcgtac cgatgcctat gcagcggtga
     1801 tgagcgacaa gcaggacacg ctgtccagcg gcaacgccta ctcgctgggc ctgcgccaca
     1861 ggttctgagc gctcccaggg ctcggcacgc cgagcccgac tcttgagcac ccccagggcc
     1921 gtgctgcgcc cagcccgccc cccgcggggg tcaaagaaac ttggggcggc ctggcgtttc
     1981 cttgagaggc gtagcgtggg ccgccgccgc gcttcagccc agcttgagcc gcgtgcggat
     2041 cgcgcccgcc cggtccgcct tggcggacag ggtcagctcg gcgcccgcgg gtgagcgcac
     2101 cagccattcg gctcgcgcac ggtcggccgt gatctcgcgg gtgggcagga aggcctgcag
     2161 cgaggccttg ggcgcatggc cttcgagctg cacgccctcg ctgatgcggg gcttgccgct
     2221 gacgaggctg gcgtccggtg gcagatcgat ctcgaagatc agcccacgca cctgttcgcg
     2281 tgccagggcc cgcttgctga cgtaggcggg caggtagccg gcgttgccga cgtcgagcgt
     2341 gacgcgccag cggctttcgc cgccgctgcc gcccagatgt tcggcgcgcg ccgacagcag
     2401 ctccaacctg ggcagcgaga gcgccagcgt gttgagccat ttcgggaagc gcgctgcctc
     2461 gcgctcgcgc agatgcggtg gcgggttgcg ccagaagttc atcttgtccc agccgcctag
     2521 ctcgaccttg ccgagctggg gatgctcgaa ggggtaccag tcgacatggg cccggccgcc
     2581 gcactgctcg tcgctccatt tcagcagctt gaggtcgtcc tccacggggt ggtcgcggta
     2641 ccagtcgatc cacttgtagt cggtgacgcc ggcctccctg ttgggcgccc agatttccac
     2701 cacccagaac agcgagccca ggtgctcgta gacccagtcc tgggtgccgg tgatcacttc
     2761 cttggggtgg tacttgaact cgtggtagat gctgaccgcc ggatagccgg tgagctgctc
     2821 gcccagcttc gagaggcgct ggtacatcca caggtcctcg ggcgccatgt tctcgtcggg
     2881 ctcagttccc atgggtcgca ggatcacgcc gctgtgggtg tgaaagctca cgcccgcgcc
     2941 gatgttgggg tgcctgacga tgaagtccac catggccctc acctccggct cgctggtggg
     3001 ataggggccg gcgccggtct gctcgtgctc ctggcgccac aggctgggga agtttcggtt
     3061 gaggtccagg ccttccttgt cggggttcac gcgcaagctc aggccgtcgt agttctccag
     3121 ccgaccttcg ggcatcagcc ggtagtactc gccgccgaac tcgcccggct cgcgcggcac
     3181 catgaggcgc ggctccgtgg ggtgcttctt gtagctgccg tgcggatcgc gcacgcgcat
     3241 gaagagcacg cggccgtcgc cgtccatgtc ctgcaccgtg aggccgtcga tcgggtcctc
     3301 gtcataggga taggggcggg tggagctgcg gatgtggcgc ggcgtgtccg acagggccag
     3361 ctcggcgccg tcggggttga gccgcggcac cagatagacg gcacgggtat cgagcagctg
     3421 cgtgatctcg cggtcgccgc cgtagccgct caccagctga tgcagccagt acagcaccgc
     3481 ggtgctggcg gtcaattcgg ccgcgtggat gttgccgtcg acccagaagg cgggcttgtc
     3541 ggtgtcggcg cctgtggcct ggttggtgat cgtcaacagc cagatgtcgc ggccttcgtg
     3601 gctcttgccc aggctgccca gcttgagcag ctgaggatgg gcggcgacat aggcctgaag
     3661 cagctcggtg agctcgccgt acttgtagaa gcggtcgaat tgcggctggg gtcgcggcgg
     3721 gggtgtcgtc ggcatggcgg gggggctcaa gcaaagccaa aaggatagcc cagcatgccg
     3781 cgccggccgg gcgcgtttcg cgtagcgctt caggctgcct tgaacaggcc ctggaaatgc
     3841 cgcgacaccg gcagcttctc gtcgcgcccc accagcatca gcaccatgtt gccgtcgtcc
     3901 tggtgctgcg cccccgcgat atggcgatgg ttgacgatca ccgagcggtg gatctgccag
     3961 aactgctcgg tgtcgagctg cggcagcagc tctttcagcg acatgcggat cagctcatcg
     4021 ccatcggaaa gcacgacgcg cgtatagcgg gcatcggact cgaagtagac gacgtcatcg
     4081 acgcgaatca agcgatgctc gcggcccagg ctggcctgga tcacttcggg cagctcgggc
     4141 ctgcgcacct ggcccagcaa ctgggtcagc aaggcatgca tatcggcggg cgggctgtgg
     4201 cgcagccggc gcttgagctt ctccagtgca tggccgagat gggcgtcgtc gacgggcttg
     4261 atcacatggt ccactgccgc ggattcgaag gcggccacgg cgcgatcgcc gggcgcagca
     4321 acgaagacca catgagcaca accgtcgatg cgcctggcca cctcgatgcc ggtcaggccg
     4381 ggcatgcgca catccagaaa gcagatctgc ggctcatgct ccaggacggc atcccaggcg
     4441 tcgacgccgt tgtcggcctc ggccacgact tgcagctcgg gccagcaccg ctgcaaggct
     4501 tcgacgagct gctcgcgcgc ggcctcttcg tcgtcggcga ccagggcggt gacggatgtg
     4561 ctcgtcatgg aagaggctcg gcgatgcggc ggtttccgca aagtgttggg tgacccatga
     4621 attgttggtg catgcgcgcc gcggcgccac tggcaagact gacaggcgcg ccacccattt
     4681 cggggggacg accaaggtca cgaccccgac tgagtgcata tggacaaatt gcccatttga
     4741 gtgcggggtc ctgagggctc gcctgggccg gccggcgagg cctcaatgtg ccaacgccgc
     4801 cccgttgacg aaggccctga gctcgccggg cgcaaaggcc gtccactgct cgtcgcgggt
     4861 cagcggctcg gtgacgacca cggcgacgcg gtcgctgggc gttgtgacgg ccgcaaagtc
     4921 gaccgtcagg tcttcgtcct gcagctgcgc atggcggaag gggtgctggc gcaccagcca
     4981 atgcagctgc gtcgagcaat gggcccatag cgcctggccg ttgctcagca tgaagttgaa
     5041 gttgccatgc gccgcgattc ccggcaccag ctcgcgcagc gtttgcgtca gcgcttccac
     5101 cttgggcacc gtcgcgtggt tcttggccag ctcctgcagc agccagcaga aggcctgctc
     5161 actgtcggtg tcgcccaccg gatggaaggc ggcatgcagc ctggggctgt agcccttgag
     5221 atcgccgttg tgcgcgaaca cccagtagcg tccccagagc tcgcgcacga aggggtggca
     5281 gttttccagg ccgacgcggc cctgggtggc cttgcggata tgcgcgatca cgttctggct
     5341 gcggatcgga taggccttga ccatgtcggc caccggcgag gcgctggcgg cctggtcgtc
     5401 gacgaagagg cgcacgccct tgtgctcgaa gaaggcgatg ccgaagccgt ccttgtgctc
     5461 ggccgagcgc gacgcgaaac cggtgaagct gaacatcacg tcggtaggcg tgttgcagtt
     5521 catgccgagc agctggcaca tcgccgtgac ctctagctga tgggcaacaa gagccgcgcc
     5581 aggcccttca gggtaaccag gttgcccgga ttgttggtct cttcgagcac gccgctcatg
     5641 cgcctgagga tgcgctcgct gcgcttggcg cgccagatca gcagatcggc cagccagggg
     5701 tgctcgttga cgcggttggc tttctcatac aggtcgaagc gcggcttgag cgcgagcagc
     5761 tcgctttcgt agcgctgcct gaccaccacc tcgtccagct tctcgtgccg cccctgcagc
     5821 acggcctcgg cggccagcat gccggtctcc attgccttgc cgatgccctc gccggtgaag
     5881 gcataagtgc tgcccacggc ctcgccggtc gcgatcaggc cggggcgcga gtacttggcg
     5941 ccttccagcg agcagcgcag cggcgcgccc ttgagctcgc ccagcagcgt gccgccttgc
     6001 gtcagctcgc gcgccggttc gtagaactcg cagaaggcgg cgaaggtctc gcgcaggttc
     6061 acgtcctgca tcgtgcggcg gccgtcgttg aacttgctgt gactgtgcgc gaggccgacg
     6121 ccgatgttga acaccccgtc gcggcaggga aagatccagc cgtagccggg gcgcagcttg
     6181 cggtgccaga ccacgtccaa cgccttgagc ctgtcgctca tggcctcgtt gcgcacatag
     6241 ccgcgcagcg cgacaccgct cggcgtgcgg cgcgtgcaca ggccggcggc gatcagggcc
     6301 tgggggaccg cgccggtggc aaggatgagc cagtcggcgt gaatttcatg cacggcaccg
     6361 ccgagcttca gcttcgcgcc gatcgc
//
LOCUS       DASUCY010000574         2947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_125622, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000574 DASUCY010000000
VERSION     DASUCY010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125622"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="VFV25_11335"
     CDS             complement(<1..770)
                     /locus_tag="VFV25_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393964.1"
                     /translation="MRRRFIALCISLLCAATAAPAHAFVSVSIGINVPVYPQMVVVPG
                     YPVYYAPTLGANFFFYDGMYWVLEDDNWYMSSWYNGPWVIVEPDYVPYYVLRVPVRYY
                     MAPPPYFRGWQPSAPPRWGNRWGPRWEQQHHDWDRWNRGAAPRPAPLPAYQRGYSGDR
                     YPSPAQQTRLQTGNYHYQPRTEVARQHVQQQQVERRGPAVAQQRPQMERPQIQRAPQM
                     APQRQQEWPTQQEPRGGGQPRYEAGPRMQPQQMSPRQAP"
     gene            complement(888..1019)
                     /locus_tag="VFV25_11340"
     CDS             complement(888..1019)
                     /locus_tag="VFV25_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393965.1"
                     /translation="MSTSRLRYDEQVLAFGNRARLDLIPGMAEHALPLKTVGDALHG"
     gene            1308..1421
                     /locus_tag="VFV25_11345"
                     /pseudo
     CDS             1308..1421
                     /locus_tag="VFV25_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653084.1"
                     /note="converts acetoacetate to acetone and carbon
                     dioxide; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase"
     gene            1418..2572
                     /locus_tag="VFV25_11350"
     CDS             1418..2572
                     /locus_tag="VFV25_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY1393966.1"
                     /translation="MNAAAYRAAVRDKKARGRAQELPGQVVLVLQGGGALGAYQVGVY
                     EALHQADIEPDWVIGTSIGAINGALIAGNPPARRLERLHEFWDRIERASPTQEALRLW
                     PEAQNFFANLDTLLRGVPGFFAPNAAAWLGAQAPVGVEAASYYSTAQLHGSLVSLVDF
                     ELLARCETRLTVGAVNVRSGSMRYFDSRDTTLRAEHVMASGALPPAFPAIRVDGEPYW
                     DGGIYSNTPIEAVLDDKPRRDSVIFSVNVWQPDGQEPESIWQVMGRQKDIQYASRADS
                     HIARQQQIHHLRHVIRELARHLPPKERESAAMKELVSWGCGTTMHVVRLLAPALAGED
                     HTKDIDFSPTGMRARWHAGYTDTLKVIERAPWREPVDPIEGVIVHQVHGA"
BASE COUNT          472 a          962 c         1016 g          497 t
ORIGIN      
        1 ggcgcttgcc gcggcgacat ctgctgcggc tgcatgcgcg ggccggcctc gtaccgcggc
       61 tggccgccgc ctctgggctc ctgctgcgtc ggccactctt gctgacgctg cggcgccatt
      121 tgcggcgcgc gctggatctg cggccgttcc atctgcggcc gctgctgtgc gaccgcgggg
      181 cctctgcgtt cgacctgttg ctgctgcacg tgctgccgcg cgacctcggt gcgcggctgg
      241 tagtggtagt tgccagtctg cagcctggtc tgctgcgcag ggctgggata gcggtcgcct
      301 gaatagccgc gctggtaggc gggcagcggg gccggtcttg gtgcggcacc gcggttccag
      361 cggtcccagt cgtggtgctg ctgttcccat cgcgggcccc agcggttgcc ccagcgtggt
      421 ggggcgctgg gctgccagcc tcggaaatac ggtggcgggg ccatgtagta gcgcaccggc
      481 acgcgcagca cgtagtaggg cacgtagtcg ggctccacga tgacccatgg gccgttgtac
      541 caggagctca tgtaccagtt gtcgtcttcg agcacccagt acatgccgtc gtagaagaag
      601 aagttggcgc cgagggtggg cgcgtagtag accgggtagc ccggcacgac caccatctgc
      661 ggatagaccg gaacgttgat gccgatgctc acgctgacga aggcatgggc gggggccgca
      721 gtcgcggcgc aaagaagtga aatgcacaag gcaatgaatc tgcggcgcat ggcctgcctc
      781 catttctgtc cttggagcgg aagtgcggct cgcggcgggc gccgcggcca cggggcttcc
      841 ccaattctgc tcgctgccca tggaggcatg gctagaagat gtatccgtca gccgtgcagc
      901 gcgtcgccca ccgtcttcaa gggcagcgca tgctcggcca tgccggggat cagatcgagc
      961 cgcgcgcggt tgccgaaggc cagcacttgt tcgtcgtagc gcagccttga agtggacatg
     1021 ggcatctcct tgaacctcgt ggtagtcggt ggattggcgc ccggcctaca ccgcgccaga
     1081 tttctcgggg gcgagcgtaa ggaagcccgc actggtgcga cagtcgctac gacccctcag
     1141 acaggagcag ccacatgttc aatggaaaga ccgccttgct cactggctcg acctcgggca
     1201 tcggactcgg catgcacact ggccgcgcaa agcgcgaccc tcgtgctcat tggcttcgga
     1261 gacgcggccg aaatcgagtc cttgcgcgcc tcgctggcca agtcaacgcg ccggtggccg
     1321 atctgccggt gaagcgcgtg atcggtgtgc tgcatttcat tgccgacctg acgctgccct
     1381 acggccgcgt gctgcacgac taccgcaagg tgatggcatg aacgcggccg cctaccgcgc
     1441 cgcggtgcgc gacaagaagg cgcggggccg cgcgcaagag ctgcccggcc aggtggtgct
     1501 ggtgctacag ggcggtggcg cgctgggcgc ctaccaggtg ggcgtgtacg aggcgctgca
     1561 ccaggcggac atcgagcccg actgggtgat cggcacgtcg atcggcgcga tcaatggcgc
     1621 actgatcgcg ggcaacccgc ccgcgcgccg cctcgagcgc ctccacgagt tctgggaccg
     1681 catcgaaagg gcctcgccca cgcaagaggc tctgcggctg tggcccgagg cgcagaactt
     1741 cttcgccaac ctcgacacgc tgctgcgcgg cgtgcccggc ttcttcgcgc ccaacgccgc
     1801 ggcgtggctg ggcgcgcagg cgccggtggg cgtggaagcg gcgtcctact acagcaccgc
     1861 gcagttgcac gggagcctgg tttcgctggt cgacttcgaa ttgctcgccc gctgcgagac
     1921 gcggctgacg gtcggtgcgg tcaacgtgcg cagcggcagc atgcgctact tcgacagccg
     1981 cgacacgacc ttgcgtgccg agcatgtgat ggcctcgggc gcgctgccgc cggccttccc
     2041 ggcgatccgc gtcgacggcg aaccctactg ggacggtggc atctactcca acacgccgat
     2101 cgaggctgtg ctcgacgaca agccgcgccg agactcggtg atcttctcgg tcaatgtctg
     2161 gcagcccgac ggccaggagc ccgaatcgat ctggcaggtg atgggccggc agaaggatat
     2221 ccagtacgcg agccgcgcag acagccacat cgcgcgccag cagcagatcc accatctgcg
     2281 ccacgtgatc cgcgagctgg cgcgccacct gcccccgaag gaacgcgaat cggcagcgat
     2341 gaaggagctc gtctcctggg gctgcggcac gacgatgcac gtggtacgcc tgctcgcccc
     2401 cgcgctggcg ggcgaggatc acaccaaaga catcgacttc agcccaaccg gcatgcgagc
     2461 ccgctggcac gccggataca ccgacacgct gaaggtcatc gaacgcgcgc cgtggcgtga
     2521 gccggtggat ccgatcgagg gggtcatcgt gcaccaggtg cacggcgctt gatcagatcg
     2581 gctcgccgat caggtcgtgc ccccgggtgc cgacgatgcg cgccttcgtg aactcgacgc
     2641 cgtgaagagc tgacaatcgg cgccctcaat gccggttgcg tcggcagcga tcggctactt
     2701 ccgcttcgct ttggaacccg tccttgggac tgcgcttcgc aatgctcttg aacgtcgaca
     2761 atgtggccaa gaccgtgagt acaggctggg accgcgaaca gccggaaccg ctgctgagct
     2821 gccggccaag ctatcgccat gggcacggtt gttagccgga gaacaggcca gcactgatca
     2881 gggttccata cggtgcaggt tgcatttgcg gttcagcttg ctggacactt ggttcgacgt
     2941 cacagtt
//
LOCUS       DASUCY010000575         2151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_125632, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000575 DASUCY010000000
VERSION     DASUCY010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125632"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(297..1121)
                     /gene="prmC"
                     /locus_tag="VFV25_11355"
     CDS             complement(297..1121)
                     /gene="prmC"
                     /locus_tag="VFV25_11355"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEY1393967.1"
                     /translation="MSLSVATALQAARAAGLERLDAQLLLAHVLSCDRAWLLAHGEAI
                     LTAEQRAAWQALLARRSTGEPLAYLLGEKEFHGLMLAVTPDVLVPRPDTETLVEWALD
                     AMRGLVAPRVLDLGTGSGAIALAIKAARPDARLTAVDASAEALAVAAANGRRLGLAVE
                     WLHSDWWSALEGRRFELVVGNPPYIAENDPHLAALQHEPERALTSGADGLDAIRVIVT
                     QAPGHLQPGGWLLLEHGYDQSGQLQALLRERGFVETGTRHDLAGQPRCSGGCWPGA"
     gene            complement(1118..1762)
                     /locus_tag="VFV25_11360"
                     /pseudo
     CDS             complement(1118..1762)
                     /locus_tag="VFV25_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
     gene            1746..2151
                     /locus_tag="VFV25_11365"
                     /pseudo
     CDS             1746..2151
                     /locus_tag="VFV25_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
BASE COUNT          317 a          745 c          756 g          333 t
ORIGIN      
        1 ccctgcacgc gattcggcga aagcaggccg ggcggggacg cgaccaggcc ttgatagtca
       61 tcaaccgggc cggcgattgc gcttgtgctc agcgctgcca gggcggccgc cgtcatcatc
      121 acaagggctt gcgtacgcat ggtcttcttc cttgttgggc caatgttcga aatcatgcta
      181 ctcccttgcc atcggggcgg ggagtgcaaa gtcgccacaa gagctgacgg gcggcattca
      241 tcccccgatg aagggtcggg gttgcggcgc cagcgcaaca gcgcagtcgc cgcgggtcag
      301 gcgccgggcc agcagccccc gctgcagcgg ggctggccgg ccaagtcgtg gcgcgtgccg
      361 gtttccacaa agccgcgctc ccgcagtagg gcttgcagct ggccggactg atcgtagcca
      421 tgctctagca gcagccagcc gcccggctgc aggtgtcccg gggcttgggt gacgatgacg
      481 cggatggcgt caaggccatc ggccccggat gtgagcgccc gctcgggttc gtgctgcagt
      541 gcggcaagat gggggtcgtt ctcggcgatg tagggcgggt tgccgactac cagctcgaag
      601 cgccgcccct caagcgccga ccaccagtcg ctgtgcagcc actcgactgc cagccccaac
      661 cggcggccgt tggctgcggc caccgccaga gcttccgcgc tggcatcgac cgccgtcagc
      721 ctggcatcag ggcgcgccgc cttgatggcg agcgcaatgg cgccgctgcc ggtgcccaga
      781 tcgagcacgc gcggcgcgac cagccctcgc atcgcatcca gcgcccattc gaccagggtc
      841 tcggtgtccg ggcgcggcac cagcacgtcc ggcgtgacgg ccagcatcag cccgtggaat
      901 tccttctcgc ccagcagata ggcgagcggc tcgcctgtgc tgcggcgggc gagcagagct
      961 tgccacgcgg cccgctgctc ggcggtgagg atggcctcgc cgtgggcgag cagccaagca
     1021 cggtcgcagc tcaggacatg ggccagcagc agctgggcgt ccaggcgctc caggcccgca
     1081 gcgcgggcgg cctgcaatgc agtggcaacg ctcaggctca tgccgtggcg ccggtttcca
     1141 gctctgccag ctgctcggcc gcgcgggccg cctgcaaggc tgccaccacc tcgccgaggt
     1201 cgccctccat gatgctggcg agcttgtaca gcgtgaggtt gatgcggtgg tcggtgagcc
     1261 ggccctgcgg gaagttgtag gtgcggatgc ggtcgctgcg gtcgccgctg ccgatcaggc
     1321 tcttgcgcgt agcggcttcc ttggcctggc gctcgctgcg ctccttgtcg cgcaggcgtg
     1381 cggccagcac ggccatggcc ttggctttgt tgcggtgctg cgagcggtcg tcctggcatt
     1441 cggccaccag cccggtgggc aagtgcgtga tgcgcacggc cgagtcggtc ttgttgatgt
     1501 gctggccgcc ggcgcccgag gcgcggaagg tgtcgatgcg cagctctgcc ggattgagat
     1561 gcacctcctc ggcctcgtcc ggctcgggca gcacggccac cgtgcaggcg ctggtatgga
     1621 tgcggccctg cgtctcggtg gccggcacgc gctgcacgcg gtggccgccc gactcgaact
     1681 tcagcttggc atacacctcg ttgccctcga agcgcagcac cacttccttg tagccgccca
     1741 gctccgagct gctctcggaa agcctctccg acctcggggg ctacaaggag gtggtgttcc
     1801 ggatcgaggg cgaggacgcg tacgcgcgcc tgaagttcga gtccggcggg caccgcgtgc
     1861 agcgcgtgcc cgccacggaa agccagggcc gcatccacac cagcgccgcc acgatcgcgg
     1921 tgatgcccga gcccgacgaa gccgaagacg tgcagatcaa cccggccgac ctgcgcatcg
     1981 acaccttccg cgcttcgggc gcgggcggcc agcacgtgaa caagaccgat tcggcggtgc
     2041 gcatcacgca catccccacc ggcatcgtcg ccgagtgcca ggacgaccgc tcgcagcacc
     2101 gcaacaaggc caaggcgatg caggtgctgg ccgcgcgcat ccaggaaaag g
//
LOCUS       DASUCY010000576         3565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1041490, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000576 DASUCY010000000
VERSION     DASUCY010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041490"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..125
                     /locus_tag="VFV25_11370"
     CDS             6..125
                     /locus_tag="VFV25_11370"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA domain-containing protein"
                     /protein_id="HEY1393968.1"
                     /translation="MRLLRRGPGGREPLAVQIGDTLFALRKVEAQCVQVEVAS"
     gene            226..2112
                     /locus_tag="VFV25_11375"
     CDS             226..2112
                     /locus_tag="VFV25_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HEY1393969.1"
                     /translation="MSQAAMDLSACRIALVGNPNCGKTALFNLLTGARQKVANYAGVT
                     VERKLGLARTAAGHTVSIIDLPGTYSLTPATPDEQVTLEVVLGQREGEAAPDLIVAVV
                     DATNLLMNLRLVLELKALGRPVIVSLNMADVARDRGLAIDIKRLEQELGVPVVETVAV
                     RHNGHAQLLARLDQMLPRVAGQQASEQEDAAPLPLRGTAELQREVRRILALAAPGAAQ
                     FERFDHRIDAVVMHPRWGLLLLAVLLFFVFQAVFSWANLPMDAIKNGVAASGQWLSEH
                     MAEGPLRSLLADGVIAGAGSVLVFLPQILILFFFILVLEDSGYLPRAAFLLDNLMGKV
                     GLSGRAFIPLLSSFACAIPGIMATRTIANWRDRLATIMIAPLMTCSARLPVYALLIGA
                     FVPNRAVGPFNLRGLTLFALYVAGVVSAMCVAWLLKRVWMKSSYQPLMLELPPYRVPS
                     VRNLAMGLWERARIFVRRVGTIIFSLTVILWFLASYPAPPADASGPAIQYSFAGWLGQ
                     ALQHVFAPIGFNWQISIALVPGLAAREVAVGALGTVYALSSAKDAVADALMPVIAQSW
                     PLATAYSLLAWYVFAPQCLSTLAVVKRETNSWRYPLLMAGYLFALAYVASFVTYRVAL
                     WFGG"
     gene            2112..2378
                     /locus_tag="VFV25_11380"
     CDS             2112..2378
                     /locus_tag="VFV25_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393970.1"
                     /translation="MMMQSLIVALLVTGCGLYAAWTLAPKTAQRALAASLLRLKLPNV
                     IATKLSRVARSSGGCSCDGCDAGSAAPRKPQGGAAPLRFHPRRR"
     gene            complement(2514..3500)
                     /gene="ispH"
                     /locus_tag="VFV25_11385"
                     /gene_synonym="lytB"
     CDS             complement(2514..3500)
                     /gene="ispH"
                     /locus_tag="VFV25_11385"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEY1393971.1"
                     /translation="MTSESVLPRVDDVLLAEPRGFCAGVDRAIEIVERAILKFGAPIY
                     VRHEIVHNTYVVNELKAKGAVFIEDLAEVPPGATLVFSAHGVSKAVRVEAAQRGFSVF
                     DATCPLVTKVHVEVAKLHKEGYEFIMIGHKGHPEVEGTMGQLTEGIYLVEDVADVERV
                     QVQRPDRLAVVTQTTLSVDDAAEILAAVKRRFPQVREPKKQDICYATQNRQDAVKVLA
                     PQVDVVIVVGSPTSSNSNRLRELAERLGTPAYMIDAPEDLQPAWFAGRPRVGLTAGAS
                     APDILVQQVIARLKSLGAVSVRKMSGVAETVHFPLPKGLKSEPGDQPLQHLA"
BASE COUNT          501 a         1230 c         1213 g          621 t
ORIGIN      
        1 agccggtgcg cctgctgcgc cgcggcccgg gcggacgcga gccgctggcg gtgcagatcg
       61 gcgacacgct gttcgcgctg cgcaaggtcg aggcgcagtg cgtgcaggtt gaagtcgcat
      121 catgagacat cccctatcgc gggccgccgc gccgggccgc tcccaagcaa ggcccgccct
      181 cccctcgggg gagtggacgg cgtactcgcc ggacgagggg tggtcatgag tcaagccgcg
      241 atggacctga gcgcttgccg catcgcgctg gtgggcaatc cgaactgcgg caagacggcg
      301 ctgttcaatc tgctgaccgg cgcgcgccag aaggtcgcca actacgccgg cgtcacagtg
      361 gagcgcaagc tgggcctggc gcgcacggcg gcagggcata cggtgtccat catcgacctg
      421 cccggcacct acagcctcac gccggccacg ccggacgagc aggtgacgct ggaagtcgtg
      481 ctcggccagc gcgaaggcga ggcggcgccc gacctgatcg tcgcggtggt cgatgcgacc
      541 aatctgctga tgaatctgcg cctcgtgctg gagctcaagg ccctgggccg gccggtcatc
      601 gtttcgctga acatggccga tgtcgcgcgc gatcgcggcc tcgccatcga tatcaagcgg
      661 ctggaacaag agctgggcgt cccggtggtc gagacggtcg ccgtgcgcca caacgggcac
      721 gcgcagctgc tggcccgtct cgatcagatg ctgccgcggg tcgccggcca gcaagccagc
      781 gagcaagaag atgcagcgcc gctgccgctg cgcggcacgg ccgagttgca gcgcgaagtg
      841 cgtcgcatcc tcgccctggc cgcgcccggc gccgcgcagt tcgagcgctt cgaccaccgc
      901 atcgacgccg tcgtgatgca cccgcgctgg ggtttgctgc tgctggcggt gctgctcttc
      961 ttcgtgttcc aggcggtatt ctcctgggcc aatctgccga tggacgcgat caagaacggc
     1021 gtcgcggcca gcggccaatg gttgagcgag cacatggccg agggcccgct gcgcagcctg
     1081 ctggccgacg gcgtgatcgc cggcgccggc agcgtgctgg tgttcctccc gcagatcctg
     1141 atcctgttct tcttcatcct ggtgctcgag gactcgggct atctgccgcg tgccgccttc
     1201 ctgctcgaca acctgatggg caaggtgggg ctgtcgggcc gcgccttcat tccgctgctg
     1261 tccagcttcg cctgcgcgat ccccggcatc atggccacgc gcaccatcgc caactggcgc
     1321 gaccggctcg ccaccatcat gatcgcgccg ctgatgacct gctcggcgcg gctgccggtc
     1381 tatgccctcc tgattggcgc cttcgtgccc aatcgtgcgg ttggcccctt caacctgcgc
     1441 gggctcacgc tgtttgcgct ctatgtggcc ggcgtcgtct ccgcgatgtg cgtggcctgg
     1501 ctgctcaagc gggtgtggat gaagagcagc taccagccgc tgatgctgga gctgccgccc
     1561 taccgcgtgc cgagtgtgcg caatctcgcg atggggctat gggagcgggc gcgcatcttc
     1621 gtgcgccgcg tgggcaccat catcttctcg ctcaccgtga tcctgtggtt cctggcgagc
     1681 tacccggcgc cgcccgccga tgcgagcggc ccggcgatcc agtacagctt cgccggctgg
     1741 ctgggccagg cgctgcagca tgtgtttgcg cccatcggct tcaactggca gatctcgatc
     1801 gcgctggtac ccgggctcgc ggcgcgcgag gtggcggtgg gcgcgctggg cacggtctat
     1861 gcgctgtcct cggccaagga cgcggtggcc gatgcgctga tgccggtgat cgcgcagagc
     1921 tggccgctcg ccacagccta ttcgctgctg gcctggtacg tgttcgcgcc gcaatgcctg
     1981 tcgacgctgg ccgtggtcaa gcgcgagacg aattcctggc gctatccgct gctcatggcg
     2041 ggctatcttt ttgcgctggc ctatgtggcg tccttcgtga cctaccgggt cgcactctgg
     2101 ttcggaggct gatgatgatg caaagcctga tcgtcgccct gctggtcacc ggctgcggcc
     2161 tgtacgccgc gtggacactc gcgcccaaga ccgcgcagcg cgcactggcc gccagcctgc
     2221 tgcgcttaaa gctgcctaac gtgatagcaa cgaaattgag ccgcgtggcg cgctcgagcg
     2281 gtggctgctc ctgcgatggc tgcgatgccg gcagtgcggc gccccgcaag ccccagggcg
     2341 gcgcagcgcc gctgcgcttc catccgcgcc gccgctgatg ccgccagctt tagaaggtgt
     2401 gaagatttcg ttgcgagcgg gccgaggtca ggtcgagtat cgcagccgta ctggcgtacg
     2461 gcgaggagcg caggcctgag atcggcccgc gcagcggaaa gatccgcagc ttctcaggcg
     2521 aggtgctgca agggctgatc gcccggctcg ctcttgagcc ccttgggcag cgggaaatgc
     2581 acggtctccg ccacgccgct catcttgcgc accgagaccg cgcccagtga cttcagccgc
     2641 gcgatcacct gttgcaccag gatgtcgggc gccgaggcgc cagccgtgag gcccacgcgc
     2701 ggacggcccg cgaaccaggc cggctgcagg tcctcaggcg catcgatcat gtaggccggc
     2761 gtgcccaagc gctcggccag ctcgcgcagg cggttgctgt tggagctggt cgggctgccg
     2821 accacgatca cgacgtcgac ctgcggcgcc agcaccttca ccgcgtcctg gcggttctgc
     2881 gtggcatagc agatgtcctg cttcttgggt tcgcgcacct gcgggaagcg ccgcttcacg
     2941 gcggccagga tctcggccgc gtcgtccacg ctcagcgtgg tctgcgtcac gacggccagg
     3001 cggtcggggc gctgcacctg cacgcgctcg acgtcggcga cgtcctcgac caggtagatg
     3061 ccctcggtga gctggcccat cgtgccttcg acctcggggt gtcccttgtg gccgatcatg
     3121 atgaactcgt agccctcctt gtgcagcttg gccacctcga catgcacctt ggtgaccagc
     3181 ggacaggtcg cgtcgaagac ggaaaagccg cgctgtgccg cctccacccg caccgccttg
     3241 ctcacgccgt gcgcgctgaa gaccagcgtc gcgcccggcg gcacctcggc cagatcctcg
     3301 atgaagaccg cgcccttggc cttgagctcg ttgaccacat aggtgttgtg cacgatctcg
     3361 tggcgcacgt agatgggggc gccgaacttg aggatcgccc gctcgacgat ctcgatcgct
     3421 cgatccacgc cggcgcagaa gccgcgtggc tcggccagca gcacatcgtc gacgcgcggc
     3481 agcacgctct cgctcgtcat cacagcaccg cgatcacttg cacttcgaac ctcaccggct
     3541 ggccggccag cgggtggttg aagtc
//
LOCUS       DASUCY010000577         2066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_816302, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000577 DASUCY010000000
VERSION     DASUCY010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816302"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VFV25_11390"
     CDS             <1..595
                     /locus_tag="VFV25_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEY1393972.1"
                     /translation="IVFLASGSICMSTMYAASFEALYSAFSRMHVQKTWDGIMNAPVA
                     LDDVVFAEMLWAALKSLFTATAIACVLLALGISRQPTLLLALPLLFVVGITFSSIALV
                     FNALAKGYDFFTYYFTLVLTPMTFLSGVYFPLEQMPGWLQTVSAVLPLTAAIELVRPL
                     VIGHAPAHWLQPLAVLLLYAVVGYYFALVLTRRRFFR"
     gene            692..1057
                     /gene="cutA"
                     /locus_tag="VFV25_11395"
     CDS             692..1057
                     /gene="cutA"
                     /locus_tag="VFV25_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008334169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HEY1393973.1"
                     /translation="MSETAEPFPGANARPLLVLSTAPDEASARKLARGLLEAHLAACV
                     NLLPAVQSIYWWQGQLQEASEWLLLIKTTSARFSELQARLREIHPYEVPECIALDISA
                     GLPDYLRWLNQETDKPPSR"
     gene            1099..>2066
                     /locus_tag="VFV25_11400"
     CDS             1099..>2066
                     /locus_tag="VFV25_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HEY1393974.1"
                     /translation="MIKCFALAMAAALLLAPALARAAGADDLLPPEQAFRFAARAVDE
                     QRVEVRYDIADGYYLYRQQFKFKAQAADGSDIALGEPEIPKGKVKFDETFQKELETYR
                     HAVAIRLPLAAPATAPYKLAVTSQGCADLGVCYPPMTSFAEVDPKSGAVRSLSAEQAA
                     AWGAAVPAAAALAPAAAGATTAGPAAPPTDAATAALRSGSFWLIVGVFFVAGLLLAFT
                     PCMLPMLPILSSLIVGSQAQQSVSRKRGFSLALSYSLGMALLYTLLGIAAGLAGEGLA
                     AALQKPWVLAGFAILLVLLALSMFGVYELQLPAALQGRLSASANKMP"
BASE COUNT          297 a          743 c          642 g          384 t
ORIGIN      
        1 catcgtcttc ctcgcctcgg gcagcatctg catgagcacc atgtacgccg cctccttcga
       61 ggcgctgtat tcggccttct cgcgcatgca tgtgcagaag acctgggacg gcatcatgaa
      121 cgcgccggtc gcgctggacg atgtggtctt cgccgagatg ctgtgggcgg cgctgaagtc
      181 gctcttcacg gccacggcga tcgcctgcgt gctgctcgcg ctgggcatca gccgccagcc
      241 cacgctgctg ctcgcgctgc cgctgctctt cgtggtcggc atcaccttct cgtcgatcgc
      301 gctggtcttc aacgcactgg ccaagggcta cgacttcttc acctactatt tcacgctggt
      361 gctcacgccg atgaccttcc tgtcgggcgt ctactttccg ctggagcaga tgccgggctg
      421 gctgcagacc gtgtcggccg tgctgccgct caccgccgcg atcgaactgg tgcggccgct
      481 ggtgatcggg catgcgcccg cgcattggct gcagcccttg gcggtgctgt tgctctatgc
      541 ggtggtcggc tactacttcg cgctagtgct gacgcggcgg cgcttcttcc gctaggcgcg
      601 gatatgtccg gaaacgatgg tatttcgaat cgagactccc aattacccca atgtttttgg
      661 ccgctgcagc gcctcgatcg tgctctgaaa aatgtccgaa actgctgaac ccttccccgg
      721 tgccaacgcg cggcccctgc tggtgttgag caccgcgccc gacgaggcga gcgcccgcaa
      781 gcttgcgcgg ggcttgctgg aggcacactt ggccgcctgc gtgaacctcc tgcctgcggt
      841 gcaatccatc tactggtggc aggggcagct gcaggaggcc agcgaatggc tgctgctgat
      901 caagaccacg agcgcgcgct ttagcgaact gcaggccagg ctgcgcgaga tccatcccta
      961 cgaagtcccc gaatgcatcg cgctcgacat cagcgccggc ctgcctgact atctgcgctg
     1021 gctgaaccag gaaacagaca agcccccttc acgatgagca cttccttttt cggtcccgtc
     1081 ctatcccggc cgacgcgcat gatcaagtgt ttcgcactcg cgatggccgc cgcgctgctg
     1141 ctggcgcccg cccttgcgcg agccgccggc gccgacgacc tgctgccgcc cgagcaggcc
     1201 ttccgcttcg ccgcgcgggc cgtcgacgag cagcgcgtcg aagtgcgcta cgacatcgcc
     1261 gacggctact acctctatcg ccagcagttc aagttcaagg cccaggccgc agacggcagc
     1321 gacatcgcgc tcggcgagcc cgagatcccc aagggcaagg tcaagttcga cgagaccttc
     1381 cagaaggaac tggagaccta ccgccacgcc gtggcgatcc ggctgccgct ggccgccccc
     1441 gccaccgcgc cctacaagct cgccgtcacg agccagggct gcgccgatct cggcgtctgc
     1501 tatccgccga tgaccagctt tgccgaggtc gaccccaaga gcggtgcggt tcgcagcctg
     1561 agcgccgagc aggcggcagc ctggggcgcg gcggtgcctg ctgcggccgc gctggctccg
     1621 gctgccgcag gcgctacaac cgccggaccg gccgcgccgc ccacggacgc tgccaccgcc
     1681 gcgctgcgct caggcagctt ctggctcatc gtcggcgtgt tctttgtcgc cggcctgttg
     1741 ctcgccttca cgccctgcat gctgcccatg ctgcccatcc tgtcctcgct gatcgtcggc
     1801 agccaggcgc agcaaagcgt cagccgcaag cgcggcttct cgcttgcatt gagctattcg
     1861 ctcggcatgg cgctgctcta caccctgctc ggcatcgccg ctggcctggc aggcgagggc
     1921 ctggccgcgg cgctgcaaaa gccctgggtg ctggccggct tcgcgatcct gctggtgttg
     1981 ctagccctca gcatgttcgg cgtctacgag ctgcagctgc ccgccgcgct gcaaggccgg
     2041 ctcagcgcct cggccaacaa gatgcc
//
LOCUS       DASUCY010000578         4733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1298696, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000578 DASUCY010000000
VERSION     DASUCY010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4733
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1298696"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..137
                     /locus_tag="VFV25_11405"
     CDS             <1..137
                     /locus_tag="VFV25_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEY1393975.1"
                     /translation="VVVIVSADAMTAHQSVINVMDAARRVGLQQLTFATQSSHSGAGN
                     "
     gene            151..1197
                     /gene="lpxK"
                     /locus_tag="VFV25_11410"
     CDS             151..1197
                     /gene="lpxK"
                     /locus_tag="VFV25_11410"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEY1393976.1"
                     /translation="MAASSWSSHLQQAWLSRRDPLAQALWPLSLLFAALVAGRRALHR
                     SGLLHVERVGLPVIVVGNRIVGGAGKTPTVLALLQHLRERGARPGVISRGYGRQGAGL
                     TEVTRNSSAQEVGDEPLLIHLRGGVPVMVGRDRVAAAEALRTAHPEITVIVSDDGLQH
                     LRLARDIEVLVFDERGAGNGWLLPAGPLREPLQATSTAHTQLVLYNAAAPSTALAGKL
                     ALRRLAGIAPLTQWWAGQAPQGFEVLGSRKLLALAGMARPERFFASLREAGLSFDALP
                     LPDHYDYRRLPWPQDATDVLITEKDAVKLDPARLASESPSTRVWVVTLDFDPGAAFFA
                     ALDAALNNAPTPSA"
     gene            1239..1454
                     /locus_tag="VFV25_11415"
     CDS             1239..1454
                     /locus_tag="VFV25_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEY1393977.1"
                     /translation="MDTRLLELLVCPICKGPLSLDRDRQELICPADHLAFPVRDGIPV
                     MLESAARALDEASSAGGSEPAGSTTTS"
     gene            1451..2212
                     /gene="kdsB"
                     /locus_tag="VFV25_11420"
     CDS             1451..2212
                     /gene="kdsB"
                     /locus_tag="VFV25_11420"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEY1393978.1"
                     /translation="MSYTVLIPARLASTRLPHKPLADIAGLPMIVRVAQRAALSRAAA
                     VVVAADDAQIVEACEAHGVHVLLTRADHATGSDRLAEACSLLGLDGDAVVVNVQGDEP
                     LIEPSMIDACAELLAQRSDCVMSTAAHAIAEVAEFTNPNVVKVVCDAQGRALYFSRAP
                     LPWWRDGYAAGVQRLSEPKPLRHIGLYAYRAGFLRRFPSLSAAPLEALESLEQLRVLW
                     HGERIAVHVSAERPGPGVDTPEDLERVRRILAQPA"
     gene            2323..2976
                     /gene="adk"
                     /locus_tag="VFV25_11425"
     CDS             2323..2976
                     /gene="adk"
                     /locus_tag="VFV25_11425"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEY1393979.1"
                     /translation="MRLILLGAPGAGKGTQAAFICQKYGIPQISTGDMLRAAVQAGTP
                     LGQAAKKVMDSGALVSDDIIIGLVKERIAQPDCAKGFLFDGFPRTIPQADAMKAAGVK
                     LDYVLEIDVPDEAIIERMSGRRVHVASGRSYHVKYNPPKQPGKDDLTGEDLIQREDDK
                     EETVKKRLAVYQAQTRPLVDYYSAWAATGDSNAPKYRKILGTGSVEEITARALAALA"
     gene            3075..3836
                     /locus_tag="VFV25_11430"
     CDS             3075..3836
                     /locus_tag="VFV25_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEY1393980.1"
                     /translation="MDIQGKVFIVTGGASGLGEGTARMLAREGGKVVIADLQVEKGEA
                     LAKEIGGVFVKCDVASEDDGRAVVAKAVSLGKLMGLVNCAGIAPAVKTVGKEGAHPLA
                     IYTKVITVNLIGSFNMIRLAAEAMCSNAPEAATGERGVLISTASVAAFDGQIGQAAYA
                     ASKGGVVGMTLPIARDLARNGIRNMTIAPGIFGTPMLFSMPQEVQDALAASVPFPSRL
                     GRPEDYAKLVHQIVTNDMLNGEVIRLDGAIRLAPR"
     gene            complement(3890..4666)
                     /locus_tag="VFV25_11435"
     CDS             complement(3890..4666)
                     /locus_tag="VFV25_11435"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEY1393981.1"
                     /translation="MYGLVAGRDEDLHFNSAAAQPRGVRSAGLASKSARWGLVICLSL
                     LASWAPAQQATSGSTELTPEQRAQRDADKVFHWITIHSDKPRKAKPEAKPAAVAAATT
                     RPTSSEAAAPAAKQVATASASAGRPVAGGGAAATSAAASPAAVAAGSVAPAATQLASI
                     APSAAAASAPEDDDEDENLVPVQQAAPEFPASLLRALHKGSVQVRFEVQPDGSVGKAE
                     IVKTSSVRLTSIATSTVTQWRFKPIHKPQSAIVELGFDID"
BASE COUNT          757 a         1641 c         1613 g          722 t
ORIGIN      
        1 acgtggtcgt catcgtctcg gccgacgcca tgacggcgca ccagagcgtc atcaatgtga
       61 tggacgcggc acgccgcgtg ggcctgcagc agctcacctt cgcgacccaa tcctcgcatt
      121 ccggcgcggg caactagcac cgccctagcc ttggccgcct cgagctggtc atcccacctg
      181 cagcaggcct ggctgagccg cagagacccg ctggcacagg ccttgtggcc tctgtcgctg
      241 ctgttcgcag ccctggtcgc cggtcgtcgc gcgctgcacc gcagcggctt gctgcacgtg
      301 gagcgggtgg gcctgcccgt catcgtggtg ggcaaccgca tcgtcggtgg cgccggcaag
      361 acgcccacgg tactggcgct gctgcagcat ctgcgtgaac gcggagcgcg ccccggcgtg
      421 atctcgcgcg gctatggtcg ccagggcgcc ggcctgaccg aggtcacccg caacagctcg
      481 gcgcaggagg tgggcgacga gcccctgctg atccatctgc gcggcggcgt gcccgtgatg
      541 gtcggccgcg accgcgtcgc ggcagccgaa gccctgcgta cggctcatcc cgagattacg
      601 gtcatcgtca gcgacgatgg cctgcagcac cttcggctgg cgcgcgacat cgaggtcctg
      661 gtcttcgacg agcgcggcgc cggcaacgga tggctgctgc cggccgggcc actacgcgaa
      721 ccgctgcagg ccaccagcac cgcacacacg caactcgtgc tttacaacgc cgccgcacca
      781 agcaccgcgc tggccggcaa gctggcgctg cgccggctgg ccggcatagc gccgctgacg
      841 cagtggtggg ccgggcaggc gccgcagggc ttcgaggtgc tgggttcgcg caagctgctg
      901 gcgctggccg ggatggcccg tcctgagcgc ttcttcgctt ccctgcggga ggccgggctg
      961 tccttcgatg cgctgccgct gccggatcac tacgattacc ggcggctgcc ttggccgcaa
     1021 gacgcgacgg atgtgctcat cacggagaag gatgcggtca agctcgatcc ggcccgcctt
     1081 gcgagcgaga gtccgagcac gagagtctgg gtcgtgacgc tagacttcga tcccggtgca
     1141 gccttcttcg cagcgctgga cgccgccctg aacaacgcac cgacgccgtc ggcatgacgg
     1201 cgccggccct gacggccctg acgcccctgt gaatcgccat ggacactcgc ttgctcgaac
     1261 ttctggtctg cccgatctgc aaaggcccgc tcagcctcga ccgcgacagg caggagctga
     1321 tctgccctgc cgaccacctt gccttcccgg tgcgcgacgg tatccccgtg atgctggaga
     1381 gcgccgcgcg cgcgctggat gaagcctcgt cggccggcgg ctcggagccg gcgggcagca
     1441 cgacgacgtc gtgagctaca ccgtcctgat cccggcgcgg ctcgcttcga cccgcctgcc
     1501 gcacaagccg ctggccgaca tcgccggcct gccgatgatc gtgcgcgtgg cgcagcgcgc
     1561 cgccctgagc cgcgcggccg cggtcgtcgt ggcggcggac gatgcgcaga tcgttgaggc
     1621 ctgcgaagcc cacggcgtgc acgtgctgct cacacgcgcc gaccacgcga ccggcagcga
     1681 tcgcctggcc gaggcctgca gcctgctggg cttggacggc gatgcagtgg tcgtcaacgt
     1741 gcagggcgac gaaccgctga tcgaaccttc gatgatcgat gcctgcgccg agctgctcgc
     1801 gcagcgcagc gactgcgtga tgagcaccgc cgcgcacgcc atagccgagg tggccgagtt
     1861 caccaacccc aatgtcgtca aggtggtctg cgatgcgcag ggccgcgcgc tctacttctc
     1921 gcgtgcgccg ctgccctggt ggcgcgatgg ctacgcggcc ggcgtgcagc gcctgagcga
     1981 gccgaaaccg ctgcgccaca tcggcctgta tgcctatcgg gccggtttcc tgcgccgctt
     2041 ccccagcctg agcgcggcgc cgctcgaggc cttggaatcg ctggagcagt tgcgcgtgct
     2101 gtggcatggc gagcgcatcg cggttcacgt cagcgccgag cggcccgggc ccggcgtgga
     2161 cacgccggaa gacctggagc gcgtgcgcag gatcctggcg cagccggcct gaccccgggc
     2221 aggcgccgtc agtttgacga aggcctgcgt gttatcctcg ttcgaaacaa gccggggctc
     2281 tcgatccact ctcacccggc acgatcgaaa acgaggagtt ccatgagact gatcctgttg
     2341 ggcgcccccg gcgccggcaa gggcacgcag gccgccttca tctgccagaa atacggcatc
     2401 ccgcagattt ccacgggcga catgctgcgc gccgccgtcc aggccggcac gccgctgggg
     2461 caggccgcca agaaggtcat ggattcgggc gcgctggtaa gcgacgacat catcatcggc
     2521 ctggtcaagg agcgcatcgc gcagcccgac tgcgccaagg gcttcctctt cgacggtttt
     2581 ccgcgcacca tcccccaggc cgacgcgatg aaggccgccg gcgtcaagct ggactatgtg
     2641 ctggagatcg acgtgcccga cgaggccatc atcgagcgca tgagcggacg ccgcgtgcat
     2701 gtggcctcgg gccgcagcta tcacgtcaag tacaacccgc ccaagcagcc cggcaaggac
     2761 gacctcaccg gcgaggacct gatccagcgc gaggacgaca aggaagagac cgtcaagaag
     2821 cgcctggccg tctatcaggc gcagacgcgc cccctggtcg actactactc cgcctgggcg
     2881 gccaccggcg acagcaacgc gcccaagtac cgcaagatac tgggcaccgg atcggtcgaa
     2941 gagatcacgg cccgcgcact ggcggcactg gcctgatccc gacatgatga gccgctagag
     3001 ccgcccaaga ggcggctctt tttgggattt caattgacgt ttacgtcaac gtcaaacagc
     3061 aacaggagct agcaatggac atacaaggca aggtcttcat cgtcaccggc ggcgcctcgg
     3121 gcctgggtga aggcacagcg cgcatgctgg cgcgcgaagg cggcaaggtc gtgatcgccg
     3181 acctgcaggt cgagaagggc gaagccctgg ccaaggagat cggcggcgtc ttcgtcaagt
     3241 gcgacgtcgc gagcgaggac gacggcaggg cggtcgtagc caaggcggtt tcgctcggta
     3301 agctgatggg cctggtgaac tgcgcgggca tcgcgccagc cgtcaagacc gtgggcaagg
     3361 aaggcgccca cccgctggcc atttacacca aggtcatcac ggtgaatctg atcggcagct
     3421 tcaacatgat ccgcctggcc gccgaggcca tgtgcagcaa cgccccggaa gcggccaccg
     3481 gcgagcgcgg cgtgctgatc tccacggcca gcgtagcggc cttcgacggc cagatcggcc
     3541 aggccgccta tgccgcgtcc aagggcggcg tggtcggcat gacactgccc atcgcacgcg
     3601 acctggcgcg caatggcatc cgcaacatga cgatcgcgcc cggcatcttc ggcacgccca
     3661 tgctgttctc gatgccgcag gaggtgcagg acgccctggc cgccagcgtg cccttcccca
     3721 gccgcctggg ccgacccgag gactacgcca aactggtgca ccagatcgtc accaacgaca
     3781 tgctcaatgg cgaggtgatc cgcctggacg gcgcgatacg cctggcgccg cgctaaatcc
     3841 gcaaggacgg tggcctgtgc tccgacaggc cgcctggggg agagccggct cagtcgatgt
     3901 cgaagccgag ctcgacgatg gcgctctgag gcttatggat gggcttgaag cgccactgcg
     3961 taacggtgga cgtggcgatg ctggtcaggc gcacgctgct ggtcttcacg atctcggcct
     4021 tgcccacgct gccatcgggc tgcacctcga agcgcacctg gacgctgccc ttgtgcaagg
     4081 cgcgcagcag cgaggccggg aactccggag ccgcctgctg gacggggacg aggttctcgt
     4141 cctcgtcgtc atcctccgga gccgaggccg ccgcagcact gggcgcgatg gaggccaact
     4201 gggttgctgc gggagcgacg ctgcccgcag ccacggccgc ggggctcgca gcagccgaag
     4261 ttgccgcagc gcccccaccg gcgacgggcc ggccggccga agcagacgcg gttgcgacct
     4321 gcttcgccgc cggggcagcg gcttcactcg atgtcgggcg agtagtggcc gccgccacgg
     4381 cggccggctt cgcttcgggc ttggccttgc gcggcttgtc ggagtggatc gtgatccagt
     4441 ggaagacctt gtcggcgtcg cgctgggcgc gctgctcggg cgtgagctcc gtgctgcccg
     4501 acgtcgcctg ctgagccggc gcccagctcg ccagcaggct caggcaaatg accagacccc
     4561 accgagcgct cttgctcgca aggcctgcag atcgcactcc acgcggttga gcggcagcgg
     4621 aattgaagtg caggtcttca tctcttcctg cgacaaggcc atacatggct gagctcccaa
     4681 caaggtccgg tgtggccgcg atagcggctt gtttgcaagt tgcgtgcctt gcc
//
LOCUS       DASUCY010000579         4051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_559420, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000579 DASUCY010000000
VERSION     DASUCY010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559420"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VFV25_11440"
     CDS             complement(<1..329)
                     /locus_tag="VFV25_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY1393982.1"
                     /translation="MPAPRPSTSQQAPVVIIGAGLAGLTVALHLAEQHPVVVLAKRNL
                     DEGATAWAQGGIVGVLGDDDSIESHVRDTQDAGAGLVDEETARFIAEHSAAAIEWLVA
                     RGVPFSP"
     gene            645..1964
                     /locus_tag="VFV25_11445"
     CDS             645..1964
                     /locus_tag="VFV25_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1393983.1"
                     /translation="MAAHPFERSAWPSSNPPASRVGTEVWQSTRVDETSAPTQILQDP
                     SDYLESVESQPDIGRAVGDDTRELFVSCDPALALEQQFQHLAPQYIALHDIGASVSRK
                     LLVSIAVAAGRKPQKLLIRRQGYGTTLATVEFVDCPASNGKSVRLYSTEVDADMQARQ
                     AMARTLLAHSTLGVLMVGDLPAHALSAAFKPLREAMVGPGWTCRSILVLPLTASSAVS
                     SQAAEMTLGTGVDARTTPQIARPADAWGFMGSTWNRLQDQAHPGGGGIMLGRLAASTP
                     VSAPASTPANTRPGDLRPASPATPMPIPGASPRSHTPLDRYVHELIEIAGMVSCCVFD
                     VAAGRAMAHAGAKPAADALARQGSEILLATMGAGRSLGLGGLAVPDLTVTLAQHHLLL
                     RAVPGHPGLALHAVLDKLHVNLAVARTQIQKLEQEFGGRGATSGWVV"
     gene            complement(2089..>4051)
                     /locus_tag="VFV25_11450"
     CDS             complement(2089..>4051)
                     /locus_tag="VFV25_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEY1393984.1"
                     /translation="VEGLAQRIVNGEVPESLKDKRVLVLDMAGLLAGAKYRGEFEERL
                     KAVLKEVAADEGRIILFIDELHTMVGAGKAEGAIDAGNMLKPALARGELHCIGATTLD
                     EYRKYVEKDAALERRFQKVLVDEPSVEATIAILRGLQEKYEVHHGVEITDPAIVAAAE
                     LSHRYITDRFLPDKAIDLIDEAAAKIKIEIDSKPEAMDKLDRRLIQLKIEREAVKKER
                     DEASQKRLGLIEEEIAKLGREYADLEEIWKAEKAAAQGSAQVKEEIDKLKFQIEEWKR
                     KGDFNKVAELQYGRMPELEKRLKEAQAKEAGKGESGKPHLLRTQVGAEEIAEVVARAT
                     GIPTAKLMQGERQKLLEMEAKLHQRVVGQDEAITAVSDAIRRSRAGLSDPNRPYGSFL
                     FLGPTGVGKTELCKALAGFLFDSEDHLIRIDMSEFMEKHSVSRLIGAPPGYVGYDEGG
                     YLTEAVRRKPYSVVLFDEVEKAHPDVFNVLLQVLDDGRLTDGQGRTVDFKNTVIVMTS
                     NLGSHLIMQMAGQDAELIREAVWAEVKQHFRPEFLNRIDETVVFHALDAKHIEAIAKI
                     QLKALEQRLEKLEIKLDISPQALAELAKVGFDPVFGARPLKRAIQHRIENPLSKLLLE
                     GRFGPKDVIPVDVDPVRAPGEFRFERALH"
BASE COUNT          601 a         1451 c         1301 g          698 t
ORIGIN      
        1 tcgggcgaga aaggcacgcc gcgcgcgacc agccattcga tcgccgcggc gctgtgctcg
       61 gcgatgaagc gcgcggtctc ctcgtccacc aggccggcgc cggcatcctg cgtgtcgcgc
      121 acgtgggact cgatgctgtc gtcgtcgccc agtacgccga cgatgccgcc ctgggcccag
      181 gccgtggcac cttcgtcgag attgcgcttg gccagcacca ccacgggatg ctgttcggcc
      241 agatgcaggg ccacggtgag gccggccaga ccggcaccga tgatgacgac gggggcttgc
      301 tgcgaagtcg agggacgcgg agcgggcata aggggctgcg atcctaggca tgtcgccggc
      361 cgcagatgca gcagcgatcg atcggtcgat tctatcgacc gcctcggagg caacctttgt
      421 ctgcaacgcc gcaggggttc aggcaagacc gcccccctga atgagggggg tggctgttgc
      481 aaaccctcct cagggggtaa cgacggcggg cacctgcgtg aaatcatgcc cagcaccctt
      541 cgcgacgtcc tgaaacagcg gcttacactg tccaggtcgc ccgccttctg gacccaagca
      601 ccgtgaacgc cgcaacagac ggcggcacct cagaggaccg actcatggca gcccaccctt
      661 tcgaacgctc ggcctggccg agctccaatc cgcccgcatc ccgggtgggc accgaggtct
      721 ggcaatccac gcgtgtcgac gaaacatcgg cgccgacgca gatcctgcag gacccgagcg
      781 actacctgga gtccgtggag tcccaaccgg atatcggccg cgccgttggc gacgacaccc
      841 gcgaactctt cgtctcctgc gaccccgctt tggcgctgga gcagcagttc cagcatctcg
      901 caccgcaata catcgcgctc catgacatcg gggcctcggt ctcgcgcaag ctgctggtga
      961 gcatcgccgt cgcggccggc cgcaagccgc agaagctcct gatccggcgc cagggctacg
     1021 gcaccacgct ggcgaccgtg gagttcgtcg actgcccggc cagcaacggc aagagcgtgc
     1081 gcctgtattc gaccgaggtg gatgccgaca tgcaggcgcg ccaggccatg gcgcgcaccc
     1141 tgctggcgca cagcacgctg ggcgtgctga tggtgggcga tctgccggcc catgccctga
     1201 gcgccgcctt caagccgctg cgcgaggcca tggtgggccc ggggtggacc tgccgcagca
     1261 tcctggtgct gccgctcacc gcaagcagcg ccgtgtcttc ccaggccgcc gagatgacgc
     1321 tcggcaccgg cgtcgatgcg cgcaccacgc cgcagatcgc gcgccctgcc gatgcctggg
     1381 gcttcatggg ctcgacctgg aatcgcctgc aggatcaggc ccatccggga ggcggcggca
     1441 tcatgctcgg acgcctggcc gcctcaacac ctgtttcggc gcctgcctcg acgcccgcca
     1501 acactcgccc gggcgacttg cgcccggcct cgcccgcaac cccgatgccg attcccggcg
     1561 cctcgccgcg ctcgcacacg ccgctcgacc gctatgtgca cgagctgatc gaaatcgccg
     1621 gcatggtcag ctgctgcgtg ttcgacgtgg cggccggccg ggcgatggcc cacgccggcg
     1681 ccaagccggc cgccgatgcc ctggcccgcc aaggcagcga aatcctgctc gccacgatgg
     1741 gcgccggccg cagcctgggc ctgggcggcc tggccgtgcc cgatctgacc gtcacgctgg
     1801 cgcagcatca cctgctgctg cgcgccgtgc cgggccaccc cggtctcgcg ctgcacgccg
     1861 tgctcgacaa gctgcacgtc aacctagcgg tcgcgcgcac ccagatccag aagctggaac
     1921 aggaattcgg cggacgcggg gcgaccagcg gctgggtcgt ctaggcctcg accgcgctcc
     1981 accgccgcat acatccggta ttgccaagca gacgccgcgg acccggcttt gccggtccgc
     2041 tggcgtcgcc cccttgaggg ggagcgctga aggcgctacg ggggtgggtc agtgcaaggc
     2101 ccgctcgaag cggaactccc cgggcgcgcg gacgggatcg acgtcgaccg ggatcacgtc
     2161 cttgggcccg aacctgcctt ccagcagcag cttggacagc gggttctcga tgcggtgctg
     2221 gatcgctcgc ttgagcggac gcgcaccgaa caccgggtcg aagcccacct tcgccagctc
     2281 ggccagggcc tgcggcgaga tatccagctt gatctccagc ttctccagcc gctgctccag
     2341 ggccttgagc tggatcttcg cgatcgcctc gatgtgcttg gcgtccagcg cgtggaagac
     2401 caccgtctcg tcgatgcggt tcaggaactc gggccggaaa tgctgcttca cctcggccca
     2461 caccgcctcg cgaatcagct ccgcgtcctg gcccgccatc tgcatgatga ggtgcgagcc
     2521 caggttgctg gtcatcacga tcacggtgtt cttgaagtcc accgtgcggc cctggccgtc
     2581 ggtcaggcgt ccatcgtcca gcacctgcag cagcacgttg aagacgtcgg ggtgggcctt
     2641 ctcgacctcg tcgaacagca ccacgctata gggcttgcgg cgcacggcct cggtgagata
     2701 gccaccttcg tcgtagccga catagcccgg cggcgcgccg atcagccggc tcacggagtg
     2761 cttctccatg aactcgctca tgtcgatgcg gatcaggtga tcctcgctgt cgaacaaaaa
     2821 gcccgccaag gccttgcaca gctcggtctt gcccacgccg gtggggccaa ggaagaggaa
     2881 ggacccatag ggccggttcg ggtcggacag gcccgcgcgc gagcggcgga tcgcatcgct
     2941 gacggcggtg atggcctcgt cctgccccac cacgcgctgg tgcagcttgg cttccatctc
     3001 gagcagcttc tgccgctcgc cctgcatcag cttggctgta ggaatgccgg tggcgcgcgc
     3061 cacgacctca gcgatctctt cggcgccgac ttgcgtgcgc agcagatggg gcttgccgct
     3121 ttcccctttg ccggcctcct tggcttgcgc ttccttgagc cgcttctcca gctcgggcat
     3181 gcgcccgtac tgcagctcgg cgaccttgtt gaagtcgccc ttgcgcttcc attcctcgat
     3241 ctggaacttg agcttgtcga tctcttcctt cacctgggcc gagccttggg ccgcggcctt
     3301 ctcggccttc cagatctcct ccaggtcggc atactcgcgc cccagcttgg cgatctcttc
     3361 ctcgatcagc cccaggcgct tctgcgaagc ctcgtcgcgc tccttcttga ccgcctcgcg
     3421 ctcgatcttg agctggatca gacggcggtc gagcttgtcc atcgcctcgg gcttggagtc
     3481 gatctcgatc ttgatcttgg cggccgcctc gtcgatcagg tcgatggcct tgtcgggcag
     3541 gaagcggtcc gtgatgtagc ggtggctgag ctcggccgcg gccacgatgg ccgggtcggt
     3601 gatctccacg ccatggtgca cctcgtactt ctcctgcagg ccgcgcagga tggcgatggt
     3661 ggcttccaca ctgggctcgt cgaccagcac cttctggaag cggcgctcca gcgcggcatc
     3721 cttctcgacg tacttgcggt attcgtccag cgtggtcgcg ccgatgcaat gcagctcgcc
     3781 gcgcgccagc gcgggcttga gcatgttgcc ggcatcgatg gcgccctcgg ccttgccggc
     3841 tcccaccatg gtgtgcaact cgtcgatgaa gaggatgatg cggccttcgt cggccgccac
     3901 ctccttcagc acggccttca ggcgttcctc gaactcgccg cggtacttgg cgccggcgag
     3961 cagacccgcc atgtccagca ccagcacgcg cttgtccttc agcgactccg gcacctcgcc
     4021 gttgacgatg cgctgcgcca gcccttcgac g
//
LOCUS       DASUCY010000580         4240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_414853, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000580 DASUCY010000000
VERSION     DASUCY010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414853"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..772
                     /locus_tag="VFV25_11455"
     CDS             98..772
                     /locus_tag="VFV25_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HEY1393985.1"
                     /translation="MKKYLTAITAPLLITLLTGCGHLSPRVDISGPTSARPQVAPAPA
                     VNNGSIFQAASYRPLFEDYRARLVGDTLTVQIVEKVSASQTSTSSVDKSGKLDAGITA
                     LPGISANSFALASASGSSSSTFAGKGSTASSNDFSGTITATVIEVLPNGHLIVAGEKQ
                     IGVNENVDVLRFSGQVDPRAIQPGNQVASAQIANVRIEHKGRGQQAEAQGIGWLSRFF
                     LNVLPI"
     gene            843..1976
                     /locus_tag="VFV25_11460"
     CDS             843..1976
                     /locus_tag="VFV25_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HEY1393986.1"
                     /translation="MDTRLDRLLRLLIGLGVVAASAALWWPAQADAARIKEVASVQGV
                     RSNQLIGYGLVVGLDGTGDQTTQTPFTTQSLAAMLQQMGVTVPPGTSMQLRNVAAVMV
                     TTQLPPFAQPGQLLDINVSSLGNAKSLRGGTLIATPLKGADGQIYALAQGNLVIGGAG
                     ASQGGSKVQINTLNAGRVPGGATVERAVPTPLAQGDSLQLDIDSDDYNTAREVERAIN
                     RRMGAGVAQALDGRVVRVRMPASPSERVAFLADIENLPVELAAPAAKVVINARTGSIV
                     LNQAVTLGPCAVAHGNLSVTISSMPVVSQPAPFGQGQTVVTAKTDIQIRQEANSLINV
                     PAGSKLTDVVKALNALGATPQDLLAILQAMKAAGALNAELEVI"
     gene            1976..2812
                     /gene="flgJ"
                     /locus_tag="VFV25_11465"
     CDS             1976..2812
                     /gene="flgJ"
                     /locus_tag="VFV25_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HEY1393987.1"
                     /translation="MSTGGYSAIAPLAGSGSLDTLRLGAAKDPKAAIRETAKQFEAMF
                     MQELLKSMRAGTLGDGWLDNAGSKMGTEMLDGQFAVKMSGLPGGLGEVIARQLERQLG
                     AVTTPGTAAASQPLQQQAPASAQDFVQQHQGAAKAAEAETGIPAAYILGQAAHESAWG
                     RREIRNADGSSSHNLFGIKAGPNWNGKVAEVTTTEYVGGQPRKVVAKFRAYESYEAAF
                     RDYAKLIKGSPRYAQVVAQGDSVQGFAQGLQRAGYASDPAYADKLSRVINTALRLQRT
                     LT"
     gene            2829..>4240
                     /gene="flgK"
                     /locus_tag="VFV25_11470"
     CDS             2829..>4240
                     /gene="flgK"
                     /locus_tag="VFV25_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HEY1393988.1"
                     /translation="MSGMISIGQSALFASYAALQTTSNNISNVNTPGYSRQQVDLETA
                     GGQFTGAGFFGKGVQVASVTRSYNQFLTTQANVSQAQASADSARLTQLQQLESALPTG
                     QAGIGYAAGDMLNALVDVGNNPQDPSARQVVLARAQDLVSRFNAASSQITALQAGTAT
                     DIKNNVATVNGLTAQVATLNQEIAKTQGLGQSPNDLLDQRDQLIAQINKYVQVTTIPA
                     SDGTVGLFVGGGQQLVLGTRALTLSTVADSYDPSQVRLAVNEPGGSQQVPTSALSGGA
                     IAGLMRFQDHDLVDARNLVGQMAAALSGRVNAQQALGLTLGQPPSAGPPIFATGAPQV
                     LPNTNNARAANGNFAASVSLTVSDPTQLQASDYELRSDPSNPGSYLLKRLSDGLTRQV
                     SPGATVDGFTISVSSPPPGPNDRFLLQPVGGAASGMKTVLADPNGIAAASPVVATVGA
                     ANTGTASVASLSVTSPTINPA"
BASE COUNT          741 a         1532 c         1405 g          562 t
ORIGIN      
        1 aggccatcca gacggccgac cagatgctgc agcgcctggg ccaactctcc tgagtggagt
       61 ggctggtgac caggcgtccg cgaatcacga gtcactcatg aagaaatacc tcacggcgat
      121 caccgccccg ctgctgataa cgctgctgac cggttgcggc caccttagcc cgcgcgtgga
      181 catcagcggg ccgaccagcg cgcgcccgca ggtcgccccc gcgccggccg tcaacaacgg
      241 ctccatcttc caggccgcca gctaccggcc cttgttcgag gattaccgcg cccgcctggt
      301 gggcgacacg ctgacggtgc agatcgtgga aaaagtctcg gcctcgcaga cctccaccag
      361 cagcgtggac aagagcggca agctcgatgc cggcatcacc gcgctgccgg gcatcagcgc
      421 caactccttc gcgctcgcct cggccagcgg ctcctcgtcc agcaccttcg ccggcaaggg
      481 cagcaccgcg agcagcaacg acttctccgg caccatcacc gccaccgtga tcgaggtgct
      541 gcccaacggc cacctcatcg tcgcgggcga gaagcagatc ggcgtgaacg agaacgtcga
      601 cgtgctgcgc ttctcgggtc aggtcgatcc gcgcgcgatc cagccgggca atcaggtcgc
      661 ctcggcccag atcgccaacg tccgcatcga gcacaagggg cgcggccagc aggccgaagc
      721 gcagggaata ggctggctgt cccggttctt tttgaacgtt ctgcccatct gaggacttcc
      781 agaatgaagc aactggcgac tcaccggtca ccagtcacaa gtcaccagtc attgcttcca
      841 agatggacac ccgcctagat cgcctgcttc gactgctcat cggcctcggc gtcgtcgccg
      901 ccagcgccgc gctgtggtgg cctgcgcagg ccgatgccgc gcgcatcaag gaggtggcga
      961 gcgtgcaggg cgtgcgcagc aaccagctga tcggctatgg cctcgtcgtg ggcctggacg
     1021 gtaccggcga ccagaccact cagaccccct tcacgaccca gagtctcgcg gccatgctgc
     1081 agcagatggg cgtgaccgtg ccgccgggca ccagcatgca gctgcgcaat gtcgccgccg
     1141 tgatggtgac gacccagttg ccacctttcg cccagccggg ccagctgctg gacatcaacg
     1201 tttcctcgct gggcaatgcc aagtcgctgc gcggcggcac gctgatcgcg acgccgctga
     1261 agggcgccga cgggcagatc tacgcgctcg cccagggcaa tctcgtgatc ggcggcgccg
     1321 gcgcatcgca gggcggctcc aaggtgcaga tcaacaccct caatgccggg cgcgtgccgg
     1381 gtggcgccac ggtggagcgc gccgtgccca cgccgctggc ccagggcgac agcctgcagc
     1441 ttgacatcga ttcggacgac tacaacaccg cccgcgaagt ggagcgcgcc atcaaccgcc
     1501 gcatgggtgc gggcgtggcc caggccctgg acggccgcgt ggtgcgcgtg cgcatgccgg
     1561 cctcgcccag cgagcgcgtg gccttcctgg ccgacatcga gaacctgccg gtggagctcg
     1621 cggccccggc cgccaaggtg gtgatcaatg cgcgcaccgg ctccatcgtg ctgaaccagg
     1681 cggtgacgct cgggccctgc gccgtggcgc acggcaatct gtcggtgacg atcagctcca
     1741 tgcccgtcgt cagccagccc gccccgttcg ggcaaggcca gaccgtggtc accgcgaaga
     1801 ccgacatcca gatcaggcag gaggccaact cgctcatcaa cgtgccggcc ggcagcaagc
     1861 tcaccgacgt ggtgaaggcg ctcaacgcgc tgggcgcgac gccgcaggac ctgctcgcca
     1921 tcctgcaagc gatgaaggcc gccggcgccc tcaacgccga gcttgaggtg atctgatgag
     1981 tacggggggc tacagcgcca tcgcgccgct ggctggctcc ggctcgctcg acacgctgcg
     2041 gctgggcgcg gccaaggacc ccaaggccgc catccgggag acggccaagc agttcgaggc
     2101 gatgttcatg caggagctgc tgaagagcat gcgcgccggc acgctgggcg acggctggct
     2161 ggacaacgcc ggctcgaaga tgggcaccga gatgctcgac ggccagttcg cggtcaagat
     2221 gagcgggctg ccgggcgggc tgggcgaggt catcgccagg cagctggagc gccagctcgg
     2281 ggccgtcacg acgcccggga ccgccgcagc ctcgcagccg cttcaacagc aggcgccggc
     2341 cagcgcgcag gacttcgtgc agcagcacca gggcgccgcc aaggcggccg aggccgagac
     2401 gggcatcccg gcggcctaca tcctcggcca ggcggcgcat gaatcggcct ggggccgccg
     2461 cgaaatccgc aatgccgacg gctccagctc gcacaacctg ttcggcatca aggccggccc
     2521 caactggaac ggcaaggtgg ccgaagtgac gaccaccgag tacgtcggcg gacagccgcg
     2581 caaggtggtg gcgaagttcc gcgcctacga gtcctatgag gccgccttcc gcgactacgc
     2641 gaaactcatc aagggcagtc cgcgctatgc gcaggtcgtg gcgcagggcg acagcgtgca
     2701 gggcttcgcg caaggcctgc agcgcgccgg ctatgcgagc gacccggcct atgccgacaa
     2761 gctgagccgg gtcatcaaca cagcgctgcg gctgcagcgc acgctgacct aggccgcgga
     2821 ggaacgccat gtccggaatg atctccatcg gccagagcgc gctattcgcc agctacgcgg
     2881 cgctgcagac cacgagcaac aacatctcca acgtcaacac gccgggctat tcgcgccagc
     2941 aggtcgatct ggagaccgcg ggcgggcagt tcaccggcgc cggcttcttc ggcaagggcg
     3001 tgcaggtggc ctcggtgacc cgctcctaca accagttcct caccacgcag gccaatgtgt
     3061 cgcaggccca ggcctcggcc gactcggccc ggctcacgca gctgcagcag ctcgaaagcg
     3121 cgctgcccac cggccaagcg ggcatcggct atgcggcagg cgacatgctc aacgcccttg
     3181 tcgacgtcgg caacaacccg caagatccct ccgcgcgcca ggtggtgctc gcaagggcgc
     3241 aggacctggt ctcgcgcttc aatgccgcca gcagccagat cacggcgctg caggcgggca
     3301 ccgcgaccga catcaagaac aatgtcgcga cggtcaacgg gctcacggcg caggtcgcca
     3361 cgctgaacca ggaaatcgcc aagacccagg gcctgggcca gtcgcccaac gacctgctcg
     3421 accagcgcga ccagctgatc gcccagatca acaaatacgt ccaggtcacc acgattccgg
     3481 caagcgacgg cacggtgggc ctcttcgtcg gcggcggcca gcagctggtg ctgggcacgc
     3541 gggcgctgac gctgtcgacg gtggcggata gctacgaccc atcgcaggtg cgcctggccg
     3601 tcaacgagcc gggcggcagc cagcaggtgc cgacgagcgc gctctcgggc ggcgccattg
     3661 ccggcctgat gcgcttccag gaccatgacc tcgtcgacgc gcgcaacctc gtcgggcaga
     3721 tggccgcggc gctgagcggc cgcgtcaatg cccaacaggc gctgggcctg acgctcgggc
     3781 agccgcccag cgcgggcccg cccatcttcg ccactggcgc gccccaggtg ctgcccaaca
     3841 ccaacaacgc acgcgcggcc aacgggaact ttgccgcatc ggtgtcgctg accgtgagcg
     3901 acccgacgca gctgcaggcc agcgactacg agctgcgcag cgacccgtcc aatcccggca
     3961 gctacctgct caagcggctg tccgacggcc tcacgcgcca ggtgagcccg ggcgccacgg
     4021 tggacggctt cacgatcagc gtgtcctcgc cgccgcccgg ccccaacgac cgcttcctgc
     4081 tgcagccggt gggcggcgcc gcctcgggca tgaagacagt gctcgccgat cccaacggca
     4141 tcgccgccgc gtcgccggtg gtggcgacgg tgggcgcggc caacaccggt accgcgagcg
     4201 tcgcctcgct gagcgtgacg agcccaacga tcaatccggc
//
LOCUS       DASUCY010000581         3266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_479363, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000581 DASUCY010000000
VERSION     DASUCY010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479363"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..811
                     /locus_tag="VFV25_11475"
     CDS             23..811
                     /locus_tag="VFV25_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY1393989.1"
                     /translation="MSPRPVVLVTGAAKRLGREIALELARAGCDLALHYRHSEAEARS
                     LASEAQGLGARVCTLAADLSDEAACEALLPAAVEALGRVDAVVNNAAAFEYDDVASFS
                     AAAMDRHWRANTAPAVLLARALHAHVSAHPGRQGCVVNLLDQKLWNPNPDYFSYTLSK
                     AALHAATELLARALAPAVRVVGVAPGVTLQSGPMTEAELAASQRMTPLQRSSSPADVA
                     RAVRFLIESPAVTGTTLLVDGGQHLQAQPRDVLFLARDANQKEA"
     gene            816..1211
                     /locus_tag="VFV25_11480"
     CDS             816..1211
                     /locus_tag="VFV25_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEY1393990.1"
                     /translation="MHSMLSHPHLLDCRRLFLKNYEVWINIGVHDFEKKGEQRVLINV
                     DLYVPLASTTPQHDQLHEVVDYDFMRRTIAQRIKQGHIHLQETLCDDIAALMLAHPQV
                     KAVKVSTEKPDVYPDCEAVGVEVFRLKDV"
     gene            1243..2190
                     /gene="ttcA"
                     /locus_tag="VFV25_11485"
     CDS             1243..2190
                     /gene="ttcA"
                     /locus_tag="VFV25_11485"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEY1393991.1"
                     /translation="MPASRDKNAAKTAFEANKLSKRLHRLVGQAIGDYNMIEPGDKVM
                     VCLSGGKDSYGLLDILLNLKARAPLHFDIVAVNLDQKQPGFPEHVLPDYLTKLGVPFH
                     IENQDTYSIVKRLVPEGKTMCSLCSRLRRGILYRVASELGATKIALGHHRDDMLQTFF
                     LNMFFGAKLKGMPPKLVSDDGKHVVIRPLAYVPEHDLEAWAAHRAFPIIPCTLCGSQE
                     NLQRKQIKAMLRDWEKKHPGRIENMFSALQSVVPSHLMDRNLYPFGTVQANGLADADG
                     DKAFDDEEICAPEPQAPAGAQTVIRLQADGAAPLYDEDI"
     gene            2202..2798
                     /locus_tag="VFV25_11490"
     CDS             2202..2798
                     /locus_tag="VFV25_11490"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HEY1393992.1"
                     /translation="MTTLRREILMGGLLAAACAALAGCAGVQNIVTQVSSYGTWTPAH
                     KPVSYAFDRLPSQDAQAAEQDRVESAAMPALEGAGFVAAPRDKADVLIQVGAQLTEYN
                     DRWADPFYWRGDWWYYRRHAFFSPGFGGYYSSPLYQREVGVLVRDRKTNAALYETHAR
                     YTNSWSSDNLLPAMFEAAMKDFPQPAISPRQVVVAMPR"
     gene            complement(2761..>3266)
                     /locus_tag="VFV25_11495"
     CDS             complement(2761..>3266)
                     /locus_tag="VFV25_11495"
                     /inference="COORDINATES: protein motif:HMM:NF039714.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6279 family lipoprotein"
                     /protein_id="HEY1393993.1"
                     /translation="ARTNRKFRDEYLQSDLAERREAFIKRVAERAELLYGSLTDSQRE
                     LVGREVDGSPFDPQLWDTQRRHNQQALLQALRQIKADRASPEQSITVLRPVFRRWADP
                     PDAEYRRYWQRLIAYNCEFAARLHNATTPAQRARALEKLRGWEQDLRILAGPEANAAS
                     PPRPGAG"
BASE COUNT          570 a         1105 c         1061 g          530 t
ORIGIN      
        1 tttcaccgcg ccattgtagg aagtgagccc ccgccccgtc gtccttgtca ccggtgccgc
       61 caagcggctc gggcgcgaga tcgcgctcga actggcgcgc gccggctgcg acctggcctt
      121 gcactatcgg cattccgagg ctgaggcgcg atcgctggcg agcgaggcgc agggcctggg
      181 tgcgcgggtg tgcacgctcg cagccgacct gtccgacgaa gccgcctgcg aggccttgct
      241 gcccgcggcg gtcgaggcac tggggcgggt cgatgcggtg gtcaacaacg cggcggcctt
      301 cgagtacgac gatgtcgcaa gcttcagcgc cgccgcgatg gacaggcatt ggcgcgccaa
      361 cacggcaccg gccgtgctgc tcgcgcgggc tctgcatgct cacgtgagcg cgcatcccgg
      421 gcgccaaggc tgcgtggtga acctgctcga ccagaagctg tggaatccca acccggacta
      481 tttctcctac acgctgtcca aggcggcgct gcacgcggcc accgaattgc tggcgcgggc
      541 gctcgcaccg gccgtgcgcg tggtcggcgt ggcgccgggc gtcacgctgc agtccggccc
      601 gatgaccgag gccgagctgg ccgcctcgca gcgcatgacg ccgctgcagc gctcgtccag
      661 cccggccgac gtggcgcggg ccgtgcgctt cctgatcgaa tcccccgccg tcaccggcac
      721 cacgctgctg gtggacggcg gacaacacct ccaagcccag ccgcgcgacg tgctcttcct
      781 cgcacgcgac gcgaaccaga aggaagcctg aagccatgca cagcatgctg tcccacccgc
      841 acctgctgga ctgccggcgg ctcttcctga agaactacga ggtctggatc aacataggcg
      901 tgcacgattt cgagaagaag ggcgagcagc gtgtgctgat caacgtcgac ctttacgtgc
      961 cgctggccag caccacgccg cagcacgatc agctgcacga ggtggtggac tacgacttca
     1021 tgcgccgcac catcgcgcag cgcatcaagc agggccatat ccacctgcag gaaacgctgt
     1081 gcgacgatat cgccgcgctg atgctggcgc atccgcaggt caaagcggtg aaggtttcga
     1141 ccgagaagcc cgatgtctac ccggattgcg aagcggtggg cgtcgaggtg tttcgtctaa
     1201 aggacgtctg aagtgaatac cgaagttctc gctgaagaag tagtgccggc gagccgcgac
     1261 aagaacgccg ccaagacggc attcgaggcc aacaagctca gcaagcgcct gcaccgcctg
     1321 gtgggccagg cgatcggcga ctacaacatg atcgagccgg gcgacaaggt gatggtctgc
     1381 ctgtccggcg gcaaggacag ctatggcctg ctcgacatcc tgctcaactt gaaggcccgc
     1441 gcgccgctgc acttcgacat cgtcgcggtc aatctcgacc agaagcagcc cggctttccc
     1501 gagcatgtgc tgcccgacta cctgacgaag ctcggcgtgc ccttccacat cgagaaccag
     1561 gacacctatt ccatcgtcaa gcgcctcgtg cccgagggca agaccatgtg cagcctgtgt
     1621 tcgcggctgc gccgcggcat tctctaccgc gtggcgagcg aactgggggc gaccaagatc
     1681 gcgctgggcc accaccgcga cgacatgctg cagaccttct tcctcaacat gttcttcggt
     1741 gccaagctga agggcatgcc gcccaagctg gtcagcgacg acggcaagca tgtggtgatc
     1801 cgcccgctgg cctatgtgcc cgagcacgac ctggaagcct gggccgcgca ccgcgccttc
     1861 cctatcattc cctgcaccct gtgcggcagc caggagaacc tgcagcgcaa gcagatcaag
     1921 gccatgctgc gcgactggga gaagaagcat ccgggccgca tcgagaacat gttttccgct
     1981 ttgcaaagcg tagtgccctc gcacctgatg gatcggaacc tctatccttt cgggactgtc
     2041 caagctaacg gactggccga tgccgacggc gacaaggctt tcgatgacga ggagatctgc
     2101 gcgcctgaac cccaagcgcc cgccggcgca cagacagtga tcaggctgca agcggacggt
     2161 gccgcgccgc tgtacgacga agacatttga ggagtccaac catgacaacg ctcagacgtg
     2221 aaatcctgat gggcggcctg ctggccgcgg cctgcgcagc cttggccggc tgtgcaggcg
     2281 tgcagaacat cgtcacccag gtcagcagct acggcacctg gacgccggcg cacaagccgg
     2341 tcagctacgc cttcgaccgc ctgccttcgc aggacgcgca agcggccgag caggatcgcg
     2401 tcgagtcggc ggccatgccg gcgctggagg gggcgggctt tgtggccgcc ccgcgtgaca
     2461 aggccgatgt gctgatccag gtgggcgcgc agctgaccga gtacaacgac cgctgggccg
     2521 accccttcta ctggcgtggc gactggtggt actaccgccg ccatgccttc ttctcgcccg
     2581 gcttcggcgg ctactacagc tcgccgctct accagcgcga agtgggcgtt ctggtgcgcg
     2641 accgcaagac caatgcggcc ctgtatgaaa cccatgcccg ctacaccaac tcctggagct
     2701 cggacaacct cctgccggcc atgttcgagg cggcgatgaa ggacttcccg cagcccgcga
     2761 tcagcccgcg ccaggtcgtg gtggcgatgc cgcgttagcc tccggccccg ccaagatacg
     2821 caaatcctgc tcccagcccc tcagcttttc caacgcgcgg gcgcgttgag cgggcgtggt
     2881 cgcgttgtgc agccgggcgg cgaactcgca gttgtaggcg atcaaccgct gccagtagcg
     2941 gcgatattcg gcatcgggcg gatcggccca gcggcggaac accggccgca gcaccgtgat
     3001 ggactgctcg ggtgaggcgc ggtcggcctt gatctgccgc agcgcctgca gcagggcctg
     3061 ctgattgtgg cggcgctgcg tatcccagag ctgagggtcg aagggcgagc cgtcgacttc
     3121 ccggccgacg agctcgcgct ggctgtccgt caggcttccg taaagcagtt ccgcgcgttc
     3181 ggccacgcgc ttgatgaagg cttcgcgccg ctccgccagg tcactctgca ggtattcgtc
     3241 gcggaacttg cggttggtcc ttgcat
//
LOCUS       DASUCY010000582         5307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_479371, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000582 DASUCY010000000
VERSION     DASUCY010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 5307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5307
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479371"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1384
                     /locus_tag="VFV25_11500"
     CDS             <1..1384
                     /locus_tag="VFV25_11500"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEY1393994.1"
                     /translation="RSWAVQRRVNAASLLAGGKVVASYAKRELPNYQVFDERRYFVSG
                     RDAGLQPLVFEVEGLRFGVLVCEDAWFDEPAAVTKAAGAQALLVLNASPFHLGKGAER
                     EERMAERARAVGLPLLYAHLVGGQDEFVFDGASFALDARGELCARAASFEPEELLVDV
                     QADGRVQGPVNSMPSYEAQAWSALVLGVRDYLGKNGFPGAIIGLSGGVDSALVLAVAV
                     DALGADKVRTVMMPSPYTADISWIDAREMARRLGVHYEEISITPMFEAFKASLEKNFA
                     GLAEDATEENIQARIRGTLLMALSNKSGRIVLTTGNKSEMATGYCTLYGDMAGGFAVI
                     KDVAKTLVYRLALWRNAQAAARGEEPPIPERIITRPPSAELRPDQTDQDSLPPYEVLD
                     AILARYMEDDQSIEQIVAAGYSAADVERVTRLIKINEYKRRQAPVGIRITHRGFGRDW
                     RYPITSKFRA"
     gene            1515..1853
                     /locus_tag="VFV25_11505"
     CDS             1515..1853
                     /locus_tag="VFV25_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEY1393995.1"
                     /translation="MKQITAIVKPFKLEEVREALADVGVTGLTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKVEVVVKDTDVERCIEAIIKAAKTGKIGDGKIFVTAVEQVVRI
                     RTGETDEAAV"
     gene            complement(1876..2460)
                     /locus_tag="VFV25_11510"
     CDS             complement(1876..2460)
                     /locus_tag="VFV25_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEY1393996.1"
                     /translation="MKPLSICVYCGSRLGERPEYEAGARELGRLIAEQGWQLVYGGGN
                     VGLMGILADAALAGGARVVGVIPESLMKREVGHRALSELHVVQTMHQRKQMMAERADA
                     FLALPGGIGTFEELFEAWTWRQLGYHDKPLGLLNVAGYYDGFVQFMRHTTEQGFLSAA
                     QLQTLEIDSDARQLLQRLASLAPKARAADDYTRI"
     gene            complement(2457..2837)
                     /locus_tag="VFV25_11515"
     CDS             complement(2457..2837)
                     /locus_tag="VFV25_11515"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEY1393997.1"
                     /translation="MSNPYKGRTGLDRVVRATGYSIAGLKIAYHGESAFRQEFWLAVL
                     MIPTAFWVGRSWLEVAVLVGSVLLVLIVELLNSGIEAAIDRVSYELHDLSKRAKDLAS
                     AAVFLSLILCGGIWAAALTHRFWP"
     gene            complement(2957..3433)
                     /locus_tag="VFV25_11520"
     CDS             complement(2957..3433)
                     /locus_tag="VFV25_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEY1393998.1"
                     /translation="MGRRMAAFVYEAIMLFGVSLVPGVLATLIHRFLGDTRSSLLLGQ
                     ACAFLTYGVYFVWLWTKNGQTLPMQTWKIRLVTAEGSPLSWQRAALRYLACWIWIAPA
                     AGLALLAGWSKWSALLAVLVWMLLYAASALLQAQRQFWHDILCGTRLVDWQPPAKK"
     gene            complement(3496..4293)
                     /locus_tag="VFV25_11525"
     CDS             complement(3496..4293)
                     /locus_tag="VFV25_11525"
                     /inference="COORDINATES: protein motif:HMM:NF022748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HEY1393999.1"
                     /translation="MLMHKLPATLLCAAALTALGSFGLGSQYALAAGAAHAAVRAGGP
                     SWAQLTPSQREALKPLEQDWNSIEPDRKQKWLEISSRFPRMGAAERERVQARMADWAR
                     LTPSQRGQARLNYQEAKVQASREERQARWEQYKALPEDKRQELAARADARRRAQEAAP
                     PRAAAHSNDVASGPAGQLKSNVVPNPLHSAQHPKRVAPTVVQVKPGATTILMSKRPVP
                     PVHQQTGLPKIAATPEFVDSATLLPQRGPQGAAVEPVRKPTRSSSNN"
     gene            complement(4304..4783)
                     /locus_tag="VFV25_11530"
     CDS             complement(4304..4783)
                     /locus_tag="VFV25_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3619 family protein"
                     /protein_id="HEY1394000.1"
                     /translation="MKNSKISSSSLLQPERDALEARFALRLTARLSQGSADLPHDVTE
                     RLRFAREQALERARTAKRVQTAAASTVVSTNGGAAALGASPSWWWRLAAVLPLVALVG
                     GLAIIQQVQQQEQIDAAAEIDAALLADDLPPSAYSDPGFKEFLAAAPASEANATVIQ"
     gene            complement(4780..>5307)
                     /locus_tag="VFV25_11535"
     CDS             complement(4780..>5307)
                     /locus_tag="VFV25_11535"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEY1394001.1"
                     /translation="VEKRAFKRTVYAVRDDDGALDIVQDAMIRLAEKYADRPAAELPL
                     LFQRILSNATMDWFRRQKVRNAVMQTFSDFESADSEGDFDLLETLEAADDSTGSESAA
                     DSVARAQILLAIEEEIGKLPARQREAFLMRYWEELDVAETAAAMGCSEGSVKTHCSRA
                     VHSLAKTLKAKGITL"
BASE COUNT          875 a         1714 c         1832 g          886 t
ORIGIN      
        1 gcggtcctgg gccgtgcagc gcagggtgaa tgccgcttct ctgctggccg gcggcaaggt
       61 ggtcgccagc tatgccaagc gcgagctgcc gaactatcag gtcttcgacg agcggcgata
      121 tttcgtctcc ggtcgcgacg cgggtttgca gccgctggtg ttcgaggtcg agggcctgcg
      181 cttcggcgtg ctggtctgcg aggacgcctg gttcgacgag cctgccgccg tgaccaaggc
      241 cgccggcgcc caggccctgc tggtgctcaa tgcctcgccc ttccatctgg gcaagggcgc
      301 cgagcgcgag gagcgcatgg ccgagcgcgc gcgcgccgtc ggcttgccgt tgctttatgc
      361 gcatctggtg ggcgggcagg acgaattcgt gttcgacggg gcttccttcg cgctggatgc
      421 acgcggcgag ctgtgcgccc gcgcggccag cttcgagccg gaagagctgc tggtcgacgt
      481 gcaggccgac gggcgcgtgc agggccctgt gaactcgatg ccgagctacg aggcgcaggc
      541 ctggagcgcg ctggtgctgg gcgtgcgcga ctacctcggc aagaacggct ttccgggcgc
      601 catcatcggc ctgtcgggtg gcgtcgattc ggcgctggtg ctcgcggtgg ccgtcgacgc
      661 gctgggcgcc gacaaggtga ggacggtgat gatgccctca ccctatacgg ccgatatttc
      721 gtggatcgac gcccgcgaga tggcccggcg cctgggcgtg cactacgaag agatttcgat
      781 cacgccgatg ttcgaggcct tcaaggccag tcttgagaag aatttcgccg gcctggccga
      841 ggacgccacc gaggagaaca tccaggcccg catacgcggc accttgctga tggcgctcag
      901 caacaagagc ggtcgcatcg tactgaccac cggcaacaag agcgagatgg cgaccggcta
      961 ctgcacgctc tatggtgaca tggccggcgg tttcgcggtc atcaaggacg tggccaagac
     1021 cctggtctac cgcctggcgc tatggcgcaa tgcccaggcc gcagcacggg gtgaggagcc
     1081 accgatcccc gagcgcatca tcacgcggcc gccctcggca gaattgcgcc ccgaccagac
     1141 cgaccaggac agcctgccgc cttatgaggt gctcgatgcc atcctggccc gctatatgga
     1201 agacgaccag tccatcgagc agatcgtggc ggccggctac agcgctgccg acgtggagcg
     1261 cgtcacgcgg ctcatcaaga tcaacgaata caagcgccgc caggcaccgg tgggcatacg
     1321 catcacgcat cgcggcttcg ggcgcgactg gcgctacccc atcacttcga agtttcgcgc
     1381 ctgaaactgg tgcatccgag ggcgcggcgt gcgcgacgat ggtgcgcgcg ggacttgtcg
     1441 cgcttgcgct gcacaaaggc acgaagttcc atgcttaaat tcactcagtt gccatctacc
     1501 gttcggagtg cgccatgaag cagatcactg ccatcgtcaa acccttcaag ctcgaggagg
     1561 tccgcgaggc gctggccgat gtgggcgtga ccgggctcac agtgaccgag gtcaagggct
     1621 tcggccggca gaagggccac accgagctct atcgcggcgc cgagtacgtg gtggacttcc
     1681 tgcccaaggt gaaggtcgaa gtggtggtca aggacaccga cgtcgagcgc tgcatcgagg
     1741 ccatcatcaa ggccgccaag accggcaaga tcggcgacgg caagatcttc gtcaccgcgg
     1801 tcgagcaggt ggtgcgcatc cgcaccggcg agaccgacga agcggcggtt tgatcctgca
     1861 ttcgccagtg cgcgctcaga tgcgcgtgta gtcgtcggcg gccctggctt tcggcgccag
     1921 tgatgcgagc cgctgcagca gctgccgggc gtcgctgtcg atctccagcg tctgcaactg
     1981 cgccgccgac aggaagccct gctcggtggt gtgacgcatg aattggacga agccatcgta
     2041 gtagcccgcc acgttgagca ggcccagcgg cttgtcgtgg tagccgagct ggcgccaggt
     2101 ccaggcttcg aagagttcct cgaaggtgcc gatgccgccc ggtagggcga ggaaggcgtc
     2161 ggcccgctcg gccatcatct gcttgcgctg gtgcatggtc tgcaccacgt gcagttcgct
     2221 taaagcccga tggccgacct cgcgcttcat cagcgactcg ggaatcacgc cgaccacgcg
     2281 cgcaccgccg gccaatgcgg catctgccaa gatgcccatt aggccgacat tgccgccgcc
     2341 atagacgagc tgccagccct gttcggcaat caaacggccg agctcgcggg cgccggcctc
     2401 gtattccggc cgctcgccga ggcgcgaacc gcagtagacg cagatcgaaa gcggcttcat
     2461 ggccagaagc ggtgtgtgag ggccgcggcc cagatgccgc cgcagaggat cagcgacagg
     2521 aagacggcgg cgctggcgag atccttggca cgcttggaca agtcgtgcag ttcgtagctc
     2581 acgcggtcga tcgccgcctc gatgccggaa ttgagcagct ccacgatcag caccagcagc
     2641 accgagccca cgagcacggc gacttccagc cagctgcggc cgacccagaa ggcggtggga
     2701 atcatcagca ccgcgagcca gaattcctgg cggaaagcgc tttcgccgtg ataggcgatc
     2761 ttcaggccgg caatggaata gccggtggct cgcaccacgc ggtccaggcc ggtgcggccc
     2821 ttgtaaggat tgctcatgag ccgcgattct gccgcggcct tgtgacgtgc gagagcctct
     2881 caaggaaacg ccgggccgcc gcaagttcct tgcccccaag gggggcgggc tgggcatagc
     2941 ccggctctgg gggtactcat ttcttggcgg gtggctgcca gtcgactaga cgcgtgccgc
     3001 acaggatgtc gtgccagaac tggcgctggg cctgcagcag ggcggaggca gcgtagagca
     3061 gcatccagac gaggacggcc agcagcgccg accatttgga ccagccggcc agcagcgcca
     3121 aacccgccgc gggcgcgatc cagatccagc aggccagata gcggagcgcg gcgcgctgcc
     3181 agctaagcgg cgacccctcg gcggtgacca ggcggatttt ccaggtctgc atcggcagcg
     3241 tctggccgtt cttggtccac agccaaacga aatagacccc gtaggtgagg aaggcgcagg
     3301 cctggccgag taagagactc gagcgggtgt ctcccaggaa ccggtgtatc agggtggcga
     3361 gcacgccggg gacaaggctg accccaaaca gcatgatggc ttcgtagacg aaggccgcca
     3421 tgcggcggcc catgctgggt gcttgcaggg cttgcggctg tgccgccacg gcctgcgccg
     3481 atgtacccga cgacatcagt tattgctgct gctgcgcgtc ggcttgcgca ccggctcgac
     3541 tgcggcgcct tgcgggcctc gctgcggcag cagcgtcgcg ctatcgacga actcgggggt
     3601 cgcagcaatc ttgggcaggc cggtttgctg gtgaacaggc ggcaccggcc gcttggacat
     3661 caggatcgtc gtcgcgccgg gtttgacctg caccacggta ggcgccactc gcttcgggtg
     3721 ctgcgcgctg tgcagagggt tgggcacgac attggatttc agctgacccg cagggccgga
     3781 cgcaacgtcg ttgctatgcg cggcggcgcg cggcggggcc gcctcctggg cccgcctgcg
     3841 ggcatctgcg cgtgccgcga gctcctggcg cttgtcttcg ggcaaggcct tgtactgctc
     3901 ccagcgcgcc tggcgttcct cgcggctggc ctggacttta gcttcctggt agttcagcct
     3961 ggcctggccg cgctggctgg gcgtgagtcg tgcccaatcc gccatgcgag cctgcactcg
     4021 ctcgcgctct gccgcgccca tgcgcgggaa gcgggacgag atttccaacc atttctgctt
     4081 gcgatcgggc tcgatgctgt tccagtcttg ctcgagcggc ttgagcgcct cgcgctggct
     4141 gggggtcaat tgggcccagg aaggaccgcc cgcccgaacg gccgcgtggg cagcgccggc
     4201 tgccagggcg tattggctgc ccaggccgaa gctgcccagc gccgtcaaag cagccgcgca
     4261 cagcagggtg gccgggagct tgtgcatcag catgggcccc ggtttactgg atcaccgtgg
     4321 cattggcctc ggacgcgggc gctgcggcca ggaactcctt gaagcccggg tcgctgtagg
     4381 cgctgggcgg gagatcgtcg gccagcaggg cagcgtctat ctcggcagcg gcatcgatct
     4441 gctcctgctg ctgcacttgc tggatgatcg ccagaccgcc gaccagggcg accagcggca
     4501 ggacggcagc aaggcgccac caccacgacg gcgaggcgcc cagcgcggcg gcaccgccat
     4561 tcgtggagac cacggtgctg gctgcagccg tctggacgcg cttggcggtg cgggcccgtt
     4621 ccagggcctg ctcgcgggcg aagcgcaggc gctcggtcac gtcatgggga agatcggcgc
     4681 tgccctggct caggcgggcg gtcaggcgca aggcgaagcg ggcctccagg gcatcgcgct
     4741 ccggctgcag cagggaagag gacgagattt tcgagttttt cacagcgtga ttcccttcgc
     4801 cttcagagtc ttggccaggc tgtgcaccgc gcgggagcaa tgcgtcttga cgctgccctc
     4861 cgagcagccc atggcggcgg ctgtctctgc gacatcgagt tcttcccagt aacgcatcag
     4921 gaaggcctcc cgttgacggg cgggcaattt gccgatttcc tcttcgatgg caagcagaat
     4981 ctgggcccgc gccaccgagt cagcagcgct ttcggagccg gtagagtcgt ctgccgcctc
     5041 cagagtttcc aaaagatcga aatcgccttc ggagtcggcc gactcgaagt ccgaaaacgt
     5101 ctgcatcacc gcattgcgca ccttctggcg gcgaaaccag tccatcgtgg cgttcgatag
     5161 gatgcgctgg aacagcagcg gcagctccgc cgccgggcgg tctgcgtatt tctccgccag
     5221 gcggatcatc gcgtcctgca cgatgtcgag tgccccgtcg tcgtcgcgca ccgcgtagac
     5281 ggtgcgcttg aaggcgcgct tttcgac
//
LOCUS       DASUCY010000583         2844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_302739, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000583 DASUCY010000000
VERSION     DASUCY010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302739"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..2152
                     /gene="metG"
                     /locus_tag="VFV25_11540"
     CDS             17..2152
                     /gene="metG"
                     /locus_tag="VFV25_11540"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEY1394002.1"
                     /translation="MPRKLFVTTALPYANAAFHIGHMMEYIQADIWVRFQRMQGSEVH
                     FVAADDAHGAPIMIAAEKAGKTPQAFVAEIAAGRWQYLDGFHISFDNWHSTDSPENTE
                     LAQDIYRRLRREGLIFTRDIEQFFDPVKNMFLPDRYIKGECPRCGAKDQYGDSCEVCG
                     AVYSPTELKNPYSTLTGATPVLKTSEHHFFQLSSSRCHEFLKTWTHAPGRLQPEVLNK
                     IKEWFTADEQGHGGLSDWDISRDAPYFGIEIPDAPGKYFYVWLDAPIGYLASLKNYFD
                     KGGAKAKYGETRSFDEFMADPKVEQYHFIGKDITYFHTLFWPAMLKFSGRKLPNNVFV
                     HGFITVSGEKMSKSRGTGISPLKYLELGMNAEWLRYYIAAKLNARVEDVDFNPDDFVA
                     RVNADLVGKYVNIASRAAGFVHKHFDGLLSRSDAAPKAGSPQAELQAAAQEIATLYDE
                     REYGKAVRRIMELADLVNGYWDASRPWLLAKSDVEADRQTLHTVCSQTLECFRLLSLY
                     IKPVLPALTSQAERFLNLAPQQWSDAPRSLPAGHRIQPYQHLMQRVDPKLLDALFEPP
                     AQAAAPKPEGNGGAEGAMPGGEALAPTIAIDDFAKIDLRIARIVNAEHVDGSSKLLRL
                     TLDVGEGTDPQGSPILRYVFSGIASAYKPEDLIGKLTVMVANLAPRKMKFGVSEGMVL
                     AASHADEKANPGLYVLEPWPGAMPGLRVR"
     gene            complement(2159..>2844)
                     /locus_tag="VFV25_11545"
     CDS             complement(2159..>2844)
                     /locus_tag="VFV25_11545"
                     /inference="COORDINATES: protein motif:HMM:NF017079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="spore coat U domain-containing protein"
                     /protein_id="HEY1394003.1"
                     /translation="TFHAYTDPARTAGWGNTTVSSINAQFSTSTKGQWTLNPAVIYGR
                     VDAGQTSAVPGSYSYASNAITMAVNFVKSTAGSPSDCQGLALNPTTQTLTATASVVQT
                     CTVSTTTLNFGTPGLLNANVDGTSTITTQCASGTAYQIGLDNGLHASGTTRQMAGGSS
                     ELIAYELYRDSSRTQRWGNTLNSDTVSISGTANTGAALTTTVYGRVAAQSTPSPSSYS
                     DTITVTVTY"
BASE COUNT          511 a          946 c          916 g          471 t
ORIGIN      
        1 atatttaacg caagccatgc cgcgcaagct cttcgtcacc acagccctgc cctacgccaa
       61 cgccgccttc cacatcggtc acatgatgga gtacatccag gccgacatct gggtgcgttt
      121 ccagcgcatg cagggcagcg aggtgcattt cgtggcggcc gacgacgccc acggcgcgcc
      181 gatcatgatc gccgccgaga aggccggcaa gacgccgcag gccttcgtgg ccgagatcgc
      241 ggccggtcgc tggcagtacc tggacggctt ccacatcagc ttcgataact ggcactccac
      301 cgacagcccc gagaacaccg agctggccca agacatctac cgccggctgc gccgcgaggg
      361 cttgatcttc acgcgcgaca tcgagcagtt cttcgatccg gtgaagaaca tgttcctgcc
      421 cgaccgctac atcaagggcg aatgcccgcg ctgcggcgcc aaggaccagt acggcgattc
      481 ctgcgaggtc tgcggcgcgg tgtactcgcc caccgagctg aagaacccct attccacgct
      541 gaccggcgcg acgccggtgc tcaagaccag cgagcaccac ttcttccagc tttcgtcctc
      601 gcgctgccac gaattcctca agacctggac gcacgcgccg ggccgtttgc agcccgaggt
      661 gctgaacaag atcaaggaat ggttcacggc cgatgagcag ggccatggcg gcctgtcgga
      721 ctgggacatc agccgcgacg cgccctactt cggcatcgag atcccggatg cacctggcaa
      781 atacttttac gtgtggctgg acgcgccgat cggctacctc gcgtcgctga agaactattt
      841 cgacaagggc ggcgccaagg ccaagtacgg cgagacgcgc agcttcgacg aattcatggc
      901 cgaccccaag gtcgagcagt accacttcat cggcaaggac atcacctatt tccacacgct
      961 gttctggccg gccatgctga agttctcggg gcgcaagctg ccgaacaacg tcttcgtgca
     1021 cggattcatc acagtcagcg gcgagaagat gagcaagagc cgcggcaccg gcatctcgcc
     1081 gctgaagtac ctcgagctgg gcatgaatgc cgaatggctg cgctactaca tcgccgccaa
     1141 gctcaacgcc cgggtcgaag acgtggactt caaccccgac gacttcgtcg cgcgcgtcaa
     1201 cgccgacctg gtcggcaagt acgtcaacat cgccagccgc gcagccggct tcgtgcacaa
     1261 gcatttcgac ggcctgctga gccgcagcga tgccgcaccc aaggccggca gcccgcaagc
     1321 cgagctgcag gcggccgcgc aggagatcgc cacgctctac gacgagcgcg aatacggcaa
     1381 ggccgtgcgc cgcatcatgg aactggccga cctcgtcaac ggctactggg acgcctcgcg
     1441 tccctggctg ctggccaagt ccgatgtgga ggccgaccgg cagaccctgc acacggtgtg
     1501 cagccagacg ctggagtgct tccgcctgct gagcctgtac atcaagcccg tcctgccggc
     1561 gctgacgagc caggccgagc gtttcctgaa cctcgcgccg cagcagtgga gcgatgcccc
     1621 gcgcagcctg cctgccgggc accgcatcca gccataccag cacctgatgc agcgcgtcga
     1681 ccccaagctg ctcgacgcgc tgttcgagcc gccggcgcaa gccgccgcgc ccaagcccga
     1741 gggcaatggc ggcgccgaag gcgcgatgcc gggcggcgag gcgctcgccc ccacgatagc
     1801 catcgacgac ttcgccaaga tcgacctgcg catcgccagg atcgtcaacg cagagcatgt
     1861 cgacggctcg agcaagctgc tgcgcctgac gctggacgtc ggcgagggta ccgacccgca
     1921 ggggagcccg atcctgcgct atgtcttcag cggcatcgcg tccgcctaca agcccgagga
     1981 cctgatcggc aagctcaccg tgatggtcgc caatcttgcg ccgcgcaaga tgaagttcgg
     2041 cgtcagcgaa ggcatggtgc tggccgccag ccatgccgac gagaaggcca accccgggct
     2101 ctatgtgctg gagccctggc ccggagcgat gccgggcctg cgggtgcgct gaggcgattc
     2161 agtaggtgac ggtcaccgta atggtgtcgc tgtagctgct cggcgaagga gtcgattgcg
     2221 cagcaacgcg gccatagacg gtggtggtca atgcggcgcc ggtgttggcg gtgccgctga
     2281 tcgaaaccgt gtcgctgttc aaggtattgc cccagcgctg cgtgcggctg ctgtcccggt
     2341 agagctcgta ggcgatgagc tcgctgctgc cgccggccat ctggcgcgta gtgccgctgg
     2401 catgcaagcc gttgtccaga ccgatctggt aggccgtgcc gctggcgcac tgggtcgtga
     2461 tcgtcgacgt gccgtcgacg tttgcgttga gcaggcccgg agtgccgaaa ttgagcgtcg
     2521 tcgtgctgac cgtgcaggtc tgcacgacgc ttgccgtggc tgtgagtgtc tgcgtggtgg
     2581 ggttgagcgc gaggccctgg caatccgacg ggctgccggc agtgctcttg acaaagttca
     2641 ccgccatcgt gatcgcgttg ctcgcgtaac tgtagctgcc cgggacggcc gaggtctgtc
     2701 ccgcatcgac gcggccgtag atgacagccg ggttcagggt ccactggccc ttggtggagg
     2761 tactgaactg cgcgttgatg gagctcaccg tcgtattgcc ccagcccgca gtccgggcgg
     2821 ggtcggtgta ggcgtggaag gtca
//
LOCUS       DASUCY010000584         2208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_479386, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000584 DASUCY010000000
VERSION     DASUCY010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479386"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VFV25_11550"
     CDS             <1..397
                     /locus_tag="VFV25_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HEY1394004.1"
                     /translation="ALMAAVGPVKAKFKGKLRLSDLQPPTSYTIQFEGQGGPAGFGKG
                     SASVRLEPNGPNQTTLHYSADAQVGGKIAQIGSRLIDMAAQKMAGEFFESFTRALSER
                     YGVAAPTPAAPAGMLARFWAWLRRLFGGG"
     gene            complement(430..1557)
                     /locus_tag="VFV25_11555"
     CDS             complement(430..1557)
                     /locus_tag="VFV25_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEY1394005.1"
                     /translation="MKSILLLGGGKIGGVVADLLAHASTGGQPDYRVTVADRSQTLLA
                     GLPHHAQLDTLVLDVTDGAALRAALKGRYAVLSALPYMLTLKVAQAARELGVHYLDLT
                     EDVAATRGIRELAQGANTAFMPQCGLAPGFISIVAHDLARRFETLDSVRMRVGALPAY
                     PSNALNYNLTWSTEGVINEYCEPCEAIVDGKPTEVPALEEREEFSLDGVLYEAFNTSG
                     GLGTLCETLAGKVRTLNYRTIRYPGHAAIMKALLNDLRLRDRRELLKDILENAVPATM
                     QDVVIVFVTVSGIRADESGQKRFLQDTYARKIYSHVLHGTMRSAIQITTASSLCSMLD
                     LLAQGKLPQRGFVMQEDLALADFLGNRFGQVYAARELAQAA"
     gene            1674..2114
                     /locus_tag="VFV25_11560"
     CDS             1674..2114
                     /locus_tag="VFV25_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEY1394006.1"
                     /translation="MPKTQLDNTDRALLALLRENAREPVAELARRLGLSRTTVQSRIE
                     RLERARVITGYTVKVANEIEAALVRAHVMITVAPKQAGAIEAACIKIAEVRSLHSVSG
                     SFDLLAVVAAPSIGELDALIDRIGALDGVERTTSAIVLSTRIER"
BASE COUNT          360 a          707 c          770 g          371 t
ORIGIN      
        1 ggcgctgatg gcggccgtcg gcccagtgaa ggccaagttc aagggcaagc tgcgcctctc
       61 ggacctgcag cccccgactt cctacacgat ccagttcgag ggccagggcg gcccggccgg
      121 cttcggcaag ggctcggcga gcgtgaggct ggagcccaat ggccccaacc agaccacgct
      181 gcactacagc gccgacgccc aggtgggcgg caagatcgcg cagatcggct cgcgcctgat
      241 cgacatggcc gcgcagaaga tggccggcga attcttcgag agcttcacgc gcgccttgag
      301 cgagcgctac ggcgtggccg cgccgacgcc ggccgccccg gccggcatgc tggccaggtt
      361 ctgggcctgg ctgcgccgcc tgttcggcgg cggctgatgc ggccggtgtc cacgccggcc
      421 gcttgagcct caggctgcct gagccagctc gcgcgccgcg tagacctggc cgaaacggtt
      481 gccgaggaag tccgccaggg ccaggtcttc ctgcatcacg aagccgcgct gcggcagctt
      541 gccttgagcc agcaggtcca gcatcgagca cagactggaa gccgtggtga tctggattgc
      601 gctgcgcatc gtgccgtgca gcacatggct gtagatcttg cgggcatagg tgtcttgcag
      661 gaagcgcttc tggccgctct cgtcggcacg gatgccgctg accgtgacga acacgatcac
      721 cacgtcctgc atcgtggccg gcaccgcgtt ctcgaggatg tccttcagca gctcgcggcg
      781 gtcgcgcagg cgcaggtcgt tgagcagcgc cttcatgatg gccgcatggc cggggtagcg
      841 gatggtgcgg tagttgagcg tacgcacctt gcccgccagc gtctcgcata gcgtgcccag
      901 gccgcccgag gtgttgaagg cctcgtagag cacaccatcg agcgagaact cctcgcgctc
      961 ctccagagct ggcacctcgg tgggcttgcc gtcgacgatg gcctcgcagg gctcgcagta
     1021 ttcgttgatg acgccctcgg tgctccaggt gaggttgtag ttcagcgcat tgctcgggta
     1081 ggccggcaag gcgccgacgc gcatgcgcac cgagtccagg gtttcgaagc ggcgcgccag
     1141 gtcgtgcgca acgatggaga tgaagcccgg cgccagaccg cactgcggca tgaaggcggt
     1201 gttcgcgccc tgggccagct cgcggatgcc gcgcgtggcg gccacgtctt cggtgagatc
     1261 gaggtagtgc acgcccagct cgcgcgcagc ttgcgcgacc ttgagcgtca gcatataggg
     1321 cagggcgctg agcacagcgt agcggccctt cagggcggcg cgcagggcgg cgccgtcggt
     1381 cacgtccagc accagcgtgt cgagctgggc atggtgtggc aggccggcta gcagcgtctg
     1441 ggaacggtcg gccaccgtga cgcggtagtc gggctggccg ccagtgcttg cgtgagccag
     1501 cagatcggcc acgacgccgc cgatcttgcc gccgccgagc aggaggatgg atttcatttg
     1561 ggtgtttcct tcaaagacca tggaattgtc ggcacagacc atacaaagcg cagctgtact
     1621 atggacgaga tgtcggcaat aattcggcga aacgtagtag ctaatagttc agtatgccga
     1681 aaacccaact cgacaacacc gaccgcgccc tactggccct gctgcgcgag aacgcccgcg
     1741 agcccgtcgc cgagctggcg cggcgcctgg gtctgtcgcg caccacggtg caaagccgca
     1801 tagagcggct ggagcgcgcg cgcgtcatca cgggctacac cgtcaaggtg gccaacgaga
     1861 tcgaggcggc gctggtgcgc gcccacgtga tgatcaccgt ggcacccaag caggccggcg
     1921 cgatcgaggc ggcctgcatc aagatcgccg aagtgcgcag cctgcactcg gtgagcgggt
     1981 ccttcgacct gctggccgtg gtggcggcgc catcgatcgg cgagctggac gcgctgatcg
     2041 accgcatcgg cgcgttggac ggcgtcgagc gcactacctc tgccatcgtg ctctccacgc
     2101 ggatcgagcg ctgaacttga tttccgcagg ggcttggcgc tcgcgtcatg tttgcttgtt
     2161 gctgttttgg gtagagtgat taatctcgta gcgtcgagga ggccttga
//
LOCUS       DASUCY010000585         2229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1330381, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000585 DASUCY010000000
VERSION     DASUCY010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1330381"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..159)
                     /locus_tag="VFV25_11565"
     CDS             complement(<1..159)
                     /locus_tag="VFV25_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011614930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEY1394007.1"
                     /translation="MTQSSPHEGLQASPWVQRWSGLVGAGARVLDVACGRGRHTRWFS
                     ARGCKVSAV"
     gene            complement(156..962)
                     /locus_tag="VFV25_11570"
     CDS             complement(156..962)
                     /locus_tag="VFV25_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY1394008.1"
                     /translation="MQVMVITGASEGIGAELARQLSDVHKNELALVLAARSLDKLEQV
                     ATQCRVHGTQVLVQRTDVSIEADCKALIAATVERFGRIDVLVNNAGMSAHALFEEVED
                     LAWYEQLMRINLWGSIWCTQAALPQLKASKGRIVAVSSLAGLIGVPGRTAYSATKFAM
                     TGFFEALRTEVEPQGVSVTIAYPGVVATEIRRHGFNAKGKPAGKSGLDERGAMSVEEC
                     ARLIAQGMERRQRDIVMSARGKLGRWLKLISPARVDAMARRALARDAAAR"
     gene            complement(963..1094)
                     /locus_tag="VFV25_11575"
     CDS             complement(963..1094)
                     /locus_tag="VFV25_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1394009.1"
                     /translation="MNILRTKRIAAWAAALLALAGVFIAYLRPALIVDLATRVWSCF"
     gene            complement(1091..>2229)
                     /locus_tag="VFV25_11580"
     CDS             complement(1091..>2229)
                     /locus_tag="VFV25_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1394010.1"
                     /translation="RHGFGLWLQPITMERGWSRETFAFAIAIQNLMWGLSGPFTGMLA
                     DRFGAFRVLIAGSLLYALGLVLMALSASGLAFTGSAGLLLGLAQSGTTYAVVYGVIGR
                     NVAPEKRSWAMGVTAAAGSFGQFLMIPVESWLIGGFGWQQALFILGCVALAIAPLAFG
                     LKEPAQPAHAAAHHQSIGQAVREAFGHPSFLLLMAGYFVCGFQVVFIGVHMPSYLRDK
                     GLSPQVATMALALIGLFNVFGTYTAGVLGQRLAKRHILSAIYLLRAVAIGIFLLAPLT
                     PLHVYIFSAVMGFLWLSTVPPTNAVVAQIFGVRYLSMLGGFVFLSHQIGSFLGVWLGG
                     KLYDLTGSYDIVWYISIALGVFAALINLPVNEGAIRRPAAMAAT"
BASE COUNT          408 a          765 c          731 g          325 t
ORIGIN      
        1 caccgcgctc accttgcagc cgcgcgccga gaaccagcgc gtatgccggc cgcggccgca
       61 ggccacgtcg agcacccggg ctcctgcgcc cacgagcccc gaccagcgct gcacccaagg
      121 cgatgcctgc aggccttcgt gcggcgagct ctgtgtcatc gcgcagcggc gtccctggcg
      181 agcgcccggc gcgccattgc atcgacgcgc gccgggctga tgagcttgag ccagcgccca
      241 agtttgcctc ttgcgctcat cacaatgtcg cgctgccggc gctccatgcc ctgggcgatc
      301 agccgcgcgc attcctccac gctcatcgcg ccgcgctcgt cgaggccgct cttgcccgct
      361 ggcttgccct tcgcattgaa tccgtggcgg cggatctcgg tcgcgacgac gccggggtaa
      421 gcgatggtca cgctcacgcc ctggggctcg acctcggtgc gtagtgcctc gaagaagccc
      481 gtcatcgcga acttggtggc gctataggcc gtgcgccccg gcacgccgat caggccggcc
      541 aggctggaca ccgcgacgat gcggcccttg ctggccttca gctgtggcaa ggcggcctgc
      601 gtgcaccaga tgctgcccca gagattgatg cgcatcagct gctcatacca ggccagatcc
      661 tcgacttcct cgaacagcgc atgggccgac atgccggcat tgttgacgag cacgtcgatg
      721 cggccgaagc gctcaacagt cgcggcgatc agcgctttgc agtcggcttc gatgctcacg
      781 tcggtgcgct gcaccagcac ctgcgtgccg tgcacccggc attgcgtggc cacctgctcc
      841 agcttgtcga ggctgcgcgc ggcgagcacg agtgcgagct cattcttgtg cacgtcgctg
      901 agctgccgcg ccagctcggc gcctatgcct tccgaggcgc cggtaataac catgacttgc
      961 atttaaaagc agctccagac gcgggtggcg agatcgacga tgagggcggg gcgcagatag
     1021 gcgatgaaga ccccagccag cgccagcaaa gctgcagccc aggcggcaat gcgcttcgtg
     1081 cgaaggatgt tcatgtcgcc gccattgccg cgggccgccg aatggcgccc tcgttcaccg
     1141 gtagattgat cagcgccgcg aacacgccca gcgcgatcga gatgtaccag acgatgtcgt
     1201 agctgccggt caggtcgtag agcttgccgc ccagccacac gcccaggaag ctgccgatct
     1261 ggtggctgag gaagacgaag ccacccagca tcgacagata gcgcacgccg aagatctgcg
     1321 ccaccacggc attggtcggc ggcaccgtgg acagccacag gaagcccatc acggccgaga
     1381 agatgtagac atgcaggggc gtgagcggcg ccagcaggaa gatgccgatc gcgacggcgc
     1441 gcagcagata gatcgccgac aggatgtgcc gcttggccag ccgctgcccg agcacgccgg
     1501 ccgtgtaggt gccaaagacg ttgaacaggc cgatcagcgc cagcgccatc gttgccacct
     1561 gcggagacag ccccttgtcg cgcagatagc tcggcatgtg cacgccgatg aagacgacct
     1621 ggaagccgca gacgaaatag ccggccatca gcagcaggaa gctcggatgg ccaaaggctt
     1681 cgcgcaccgc ctggccgatg ctctggtgat gcgcagcggc atgggcgggc tgggccggct
     1741 ccttcaatcc aaaggccagc ggcgcgatgg ccagggccac gcagccgagg atgaacaagg
     1801 cctgctgcca gccgaagccg ccgatcagcc agctttccac cgggatcatc aggaactggc
     1861 cgaaggagcc tgctgccgca gtcacgccca tggcccagga gcgcttctcg ggcgccacat
     1921 tgcggccgat gacgccatag accacggcat aggtcgtgcc cgactgggcc aggcccagca
     1981 gcagcccggc gctgccggtg aaggccaggc ccgaggccga caaggccatc agcaccaggc
     2041 ccagcgcata gagcaggctg cctgcgatga gtacgcggaa ggcgccgaaa cggtcggcca
     2101 gcataccggt gaaggggccg ctcagacccc acatgaggtt ctgtatcgcg atcgcgaagg
     2161 cgaaggtctc gcggctccag ccacgctcca tcgtgatcgg ttgcagccac agcccgaagc
     2221 cgtggcgta
//
LOCUS       DASUCY010000586         2217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1138025, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000586 DASUCY010000000
VERSION     DASUCY010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1138025"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1414)
                     /locus_tag="VFV25_11585"
     CDS             complement(<1..1414)
                     /locus_tag="VFV25_11585"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HEY1394011.1"
                     /translation="MAVSVFDLFKIGIGPSSSHTVGPMRAARLFALRLQHESLLPCTS
                     RVLCELYGSLGATGKGHGSDKAVLLGLAGHEPDTVDVEAVPALLAQIRDTRRLKLLGE
                     HEIAFDEGADLTFLRRQTLPFHANGMRFVAYDAQGAEIEARIYYSVGGGFVVSDEVAA
                     DGERQKRIAPDTTVLPHPFRTGAELLALAAKEACSIAEIMRRNERHWRSDDEIDAGLL
                     KIWHVMQDCVARGCRTEGVLPGGFKVKRRAAALYRDLTANPEAALRDPLQVMDWVNLY
                     ALAVNEENAAGGRVVTAPTNGASGIIPAVLHYYTRFVPGATDQGVIDFMLTAGAIGIL
                     YKENASISGAEVGCQGEVGVACSMAAGALAAVMGGTPAQAENAAGGRVVTAPTNGAAG
                     IVPAVLHYYHRFVPGANEQGVFDFLLTAAAIAILYKENASISGAEVGCQGEVGSASSM
                     AAAGLAEVMGGTPAQVENAAE"
     gene            complement(1445..>2217)
                     /locus_tag="VFV25_11590"
     CDS             complement(1445..>2217)
                     /locus_tag="VFV25_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014617654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEY1394012.1"
                     /translation="PEFFSNGSKLLVTENTSAAAQLGKLTPDAVEQLVIKRDDIHYPK
                     PKVVSLTQATELGTVYTVEEVRAIAAIAKRRQLKLHMDGARFANAVATLGCHPSDITW
                     RAGVDVLCFGGTKNGLPVGEAVVFFDRELSQDFAWRVKQAGQLASKMRFISAPWVGML
                     ENEVWLRNARHANAMAQRLYAAIKDVPGVRVMHPPQANAVFAYLPRPVIDGLHAQGWK
                     FYDFIAGGGCRLMCAWDTSTEAVDAFAADIATACARVS"
BASE COUNT          340 a          741 c          780 g          356 t
ORIGIN      
        1 tctcggccgc gttctcgact tgggcagggg tgccgcccat gacctcggcg aggcccgcgg
       61 ccgccatgga cgacgccgag cccacctcgc cctggcagcc cacctcggcg ccggagatgg
      121 aggcgttttc cttgtagagg atcgcgatgg ccgcggcagt gagcaggaaa tcgaacacgc
      181 cctgctcgtt cgcgcccggc acgaagcggt ggtagtagtg caggaccgcc ggcacgatgc
      241 ccgccgcgcc gttggtgggc gccgtgacca cgcggccgcc cgcggcgttc tcggcctgcg
      301 cgggcgtgcc gcccatcaca gctgcgagcg cgcccgcagc catcgagcag gccacgccga
      361 cctcgccctg gcagccgact tcggcgccgg agatcgaggc gttttccttg tagaggatgc
      421 cgatcgcgcc ggccgtaagc atgaagtcga tcacgccctg atcggtggcg ccgggcacga
      481 agcgcgtgta gtagtgcagc acggccggga tgatgccgga cgcgccgttg gtcggtgccg
      541 tcaccacgcg gccaccggca gcgttttcct cgttgacggc cagcgcatag aggttgaccc
      601 agtccatgac ctgcagcgga tcgcgcagcg ccgcttcggg gttggctgtc aggtcacggt
      661 agagcgctgc tgcgcggcgc ttgaccttga agccgccggg cagcacgccc tcggtgcggc
      721 aaccgcgcgc gacgcagtcc tgcatcacgt gccagatctt cagcaggccg gcgtcgatct
      781 cgtcatcgct gcgccagtgc cgctcgttgc gccgcatgat ctcggcgatg ctgcaggctt
      841 cctttgcggc cagcgcgagc agttcggcgc cggtgcggaa gggatggggc agcacggtgg
      901 tatcgggggc gatgcgcttt tgccgctcgc cgtcggccgc cacctcgtcg ctgacgacga
      961 agccgccgcc caccgagtag tagatgcgcg cctcgatctc ggcgccttgt gcgtcgtagg
     1021 ctacgaagcg catgccgttg gcgtggaagg gcaaggtttg gcggcgcagg aaagtgaggt
     1081 cggctccctc gtcgaaggcg atctcgtgct cacccagcag cttgaggcgc cgcgtgtcgc
     1141 ggatctgcgc cagcagcgcc ggcacggcct ccacgtccac cgtgtccggc tcgtgcccgg
     1201 ccaggcccag cagcacggcc ttgtcgctgc catggccctt gccggtggca cccagcgagc
     1261 catacagctc gcacagcacg cggctggtac acggcagcag gctttcatgc tgcaggcgca
     1321 gggcgaacag gcgcgcggcc cgcatcgggc ccacggtatg cgagctcgat ggcccgatgc
     1381 cgatcttgaa gaggtcgaaa acggagaccg ccatgggtcc tccacggtta ctgatactag
     1441 cgaatcaaga gacccttgcg caggccgtgg ctatgtcggc agcgaaggca tcgacggcct
     1501 cggtgctggt gtcccaggcg cacatcaggc ggcagccacc gccggcgatg aaatcgtaga
     1561 acttccagcc ctgggcgtgc aggccgtcga tgacggggcg gggcagataa gcgaagaccg
     1621 cattggcctg cggcgggtgc atcacgcgca cgccggggac gtccttgatc gccgcgtaga
     1681 gacgctgagc catcgcgttg gcgtgacgcg cgttgcgcag ccagacctcg ttctccagca
     1741 tgccgaccca gggcgccgag atgaagcgca tcttggaggc gagctggccg gcctgcttga
     1801 cgcgccaggc aaagtcctgc gacagctcgc gatcgaagaa gaccaccgcc tcgcccaccg
     1861 gcaggccgtt cttggtgccg ccgaagcaca gcacgtcgac gccggcgcgc caggtgatgt
     1921 cgctcgggtg gcaacccagc gtcgcgacgg cgttggcaaa gcgcgcgcca tccatgtgca
     1981 gcttgagctg gcggcgcttg gcgatggcgg cgatcgctcg tacctcctcc acggtgtaga
     2041 ccgtgcctag ctcggtggcc tgggtcaggc tcacgacctt gggcttggga tagtggatgt
     2101 cgtcgcgttt gataacgagc tgctcgaccg catcgggcgt cagcttgccg agctgggccg
     2161 cggcgctggt gttctctgtg acgagcagct tggagccgtt ggagaagaac tccggcc
//
LOCUS       DASUCY010000587         6042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_222577, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000587 DASUCY010000000
VERSION     DASUCY010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 6042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6042
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222577"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            261..1430
                     /locus_tag="VFV25_11595"
     CDS             261..1430
                     /locus_tag="VFV25_11595"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HEY1394013.1"
                     /translation="MACILATPGLVRADVITDWNDKACVIVGKVGPGSTGHRIMAIVQ
                     VSVYDAVSSIDRRHKPYLAKIAAPTGASVEAAVAAANHAVLAELVPGEKSAIEAVYQS
                     ALAAIPDGQAKADGISVGEKAASAILARAAADGSNVPDTYQPRTTPGVYVPTTIPVFA
                     TWTQRKPWIMARPDQFRPGPPPELSSDTWVKDYNEVKALGGKNSSQRTPQQTEIARFW
                     EETRPLIYHPLLRSVAIQSGRTVAQNARLYAAGAVAIDDALIAVYDAKYIYQFWRPIT
                     AIRNGGGNKATAQEIGWTPLIPTPMHPEYPCAHCISSSALAAVIQAEVGGGPMPVLAT
                     SSPTAGGLTHQWTNLSDLTEEVKMARIYDGVHYRNSADVGIAMGQRIGKLVVEKF"
     gene            1456..1782
                     /locus_tag="VFV25_11600"
     CDS             1456..1782
                     /locus_tag="VFV25_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014898658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HEY1394014.1"
                     /translation="MAKFLLIESRDPFDNNIVARQYDLAVNLVKEGNEVTLFLVQNGV
                     FPARPSVHSVLLTNTARSGVEVLADDFSLRERGIAGNQLAEGVKAAPLSVIVDQLADG
                     RKAIWH"
     gene            1793..2200
                     /locus_tag="VFV25_11605"
     CDS             1793..2200
                     /locus_tag="VFV25_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014898659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEY1394015.1"
                     /translation="MSKTLTFALMDPPFENARSTTAMRLIDLAVKRGFNVNVFAYEGA
                     VYLPFAKQAQHPNAVHGRSLEEENHPLPRAWIAALMKEAAAKGTKVDWINCGLCVDER
                     GAGETIEGVRRGSPVDFWKFAESSDNTLVIGTK"
     gene            2210..2590
                     /locus_tag="VFV25_11610"
     CDS             2210..2590
                     /locus_tag="VFV25_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014898660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEY1394016.1"
                     /translation="MKVLNIVEAAYRATLEEQDDPIIWICSAMKGANADIDVLLRGNA
                     VNYAIKAQDASGLSFGGQAQTQPPRIADDLVRLKGKGVKIYVVDDALRERGIEPGELI
                     DGLDFVPRSKLAKLFGGYDQVWHW"
     gene            complement(2695..3939)
                     /locus_tag="VFV25_11615"
     CDS             complement(2695..3939)
                     /locus_tag="VFV25_11615"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEY1394017.1"
                     /translation="MSSPFAALGLSPALVRAVAERGYDRPTPIQVATIPAALQGRDLV
                     GCAHTGSGKTAAFALALLQRLDNGPRPARRRTDALVLVPTRELAVQVGETIGALARHL
                     AAPLKVAVVFGGVAINPQMLALRGGAEVIVATPGRLLDLAERHALGLGDVGLLVLDEA
                     DRLLDEGFADEFARVTALLPARRQSLFFSATFPSAVQALAAALLREPLRIDVAAGPGA
                     GVPDIEQRAVEVDAAQRTPLLRHLIAQGRWQRVLVFVATRYASEHVAEKLRRAGLSAA
                     ALHGELSQGARQRVLADLNSSALRVVLATDLAARGLHVPGLPVVVNYDLPRSAVDYLH
                     RIGRTARAGANGLAVSFVSTATEAHFRLIEKRQGRRVPRERIAGFEPRESALPASPPE
                     GGVKGRRKSRKDKLREAAARGD"
     gene            complement(3951..5381)
                     /gene="dbpA"
                     /locus_tag="VFV25_11620"
     CDS             complement(3951..5381)
                     /gene="dbpA"
                     /locus_tag="VFV25_11620"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HEY1394018.1"
                     /translation="MTATSDVAPRTGPAFSTLPLASHVLENLQRLGYEHMTPIQAASL
                     PLALAGKDLIAQAKTGSGKTAAFALPLLANLNPRRFAVQALVLCPTRELAEQVTQEIR
                     RLARAEDNVKVLTLCGGATMRPQLASLAHGAHVVVGTPGRILDHVVRESLVLDALNTL
                     VLDEADRMLDMGFAEDIAAIAQHCPPTSQRQTLLFSATYPDDVARLAARYLREPQEIR
                     LNERHAASLIHQRFYEVTESERLHAVGLLLNHFRPESTLAFCNTKQQCRDLVAVLQAQ
                     GIVALELHGDLEQRERDQVLVRFANRSCSVLVATDVAARGLDIARLEAVINVEITPDA
                     QLHTHRAGRTGRAGESGLVFNLASVDEMGRVGRIDEMQGSESVWHPLAELQASGAQHA
                     APGEPLRPPMVTLQILGGRKEKIRPGDVLGALTKDLGLDAAAIGKIDVNDFSTYVAVQ
                     RDIAERACRGLNDGKVKGRSVKVRRL"
     gene            complement(5416..5574)
                     /locus_tag="VFV25_11625"
     CDS             complement(5416..5574)
                     /locus_tag="VFV25_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1394019.1"
                     /translation="MSTTSAGLCGRRRGRGDGGRCATAQRLGASHAQRDLLELTLPAE
                     HWRARVAR"
BASE COUNT         1016 a         1999 c         2059 g          968 t
ORIGIN      
        1 caggtgaatt tcggctcttg gccgggtcac tcgcccagag agccgagccg gagtcgaggc
       61 cactaccagc gcccagacac cagcaagcgc tgtccctgtt ttgcaggaaa tccgtcccga
      121 tgcctcgttt gagcgtagaa acccatcgca ccgggaacta gactgcttgc agacagggag
      181 gccgtgccca tctcggtggc cccaccattg ggagacagcc aatgaggaac gcgtcaaggg
      241 caggaatcgc gggcctcacc ttggcctgca tactcgccac gccaggtctc gtgcgggccg
      301 atgtgatcac cgattggaac gacaaggcct gtgtcatcgt tggcaaggtc ggtcctggct
      361 ccacggggca tcggatcatg gccattgtcc aggtctcggt gtacgacgcg gtcagttcca
      421 tcgaccggcg acacaagccc tacctcgcaa aaatcgcggc gcccacgggc gcatcggtcg
      481 aagccgcggt ggctgcggcg aaccacgccg tgctcgccga actcgtgccg ggtgaaaaat
      541 ccgctattga ggcggtctat caatcggccc tggccgcgat ccccgacggg caggcgaagg
      601 cggacggcat ttcggtgggc gagaaggcgg cgtcggccat cctcgcgcgc gcggcggccg
      661 acggtagcaa tgtgccggac acataccagc cgcgaacgac gcccggcgtc tatgtgccca
      721 ccaccatccc ggtcttcgcc acctggaccc agcgcaagcc atggatcatg gcacgccctg
      781 accagttccg ccccggcccg ccgccggaac tcagcagcga tacctgggtg aaggactaca
      841 acgaggtgaa ggcgttgggg ggcaagaaca gctcgcaacg caccccgcag cagaccgaga
      901 tcgcgcgctt ctgggaagaa acgcggccgc tcatctatca ccccttgctg cgctcagtcg
      961 cgatccagtc ggggcggacc gtggctcaga acgcgcgcct ctatgccgcg ggggcggtgg
     1021 ccattgacga tgcgctgatt gccgtctacg acgccaagta catctatcag ttttggcgcc
     1081 cgatcaccgc gatccgcaac ggcgggggta acaaagccac tgcgcaggag atcggctgga
     1141 cgccgctcat cccgacgccc atgcaccctg aatatccctg cgcccactgc atctcctcga
     1201 gcgcgctcgc tgcggtcatc caagcggagg ttggcggcgg cccaatgcca gtgctagcca
     1261 cctcgagccc aacggcgggc gggctgaccc atcaatggac gaacctttcg gacctcaccg
     1321 aagaagtgaa gatggctcgc atctacgacg gcgttcatta ccgcaattcc gcagacgttg
     1381 gcatcgcgat gggtcagcga attgggaagc ttgtggtaga gaagttttga aacgggagaa
     1441 cgaaaggaga tagacatggc gaagttcttg ctcatcgaat cccgcgaccc gttcgacaac
     1501 aacatagtgg cgcggcaata cgatttggca gtaaacctgg tcaaggaagg caatgaggtg
     1561 accttgttcc tggttcagaa cggtgtgttc ccggcgcgcc ccagcgtgca ctcggtactc
     1621 ttgaccaata cggcgcgctc cggcgtggaa gtgctggcgg acgatttttc gctgcgcgag
     1681 cgcgggattg caggtaacca gctggcggag ggtgtgaagg cagcgccact ttccgttatc
     1741 gtcgatcagc tcgccgacgg ccgcaaggcc atctggcact gaggaggccg ccatgtcaaa
     1801 gacactcacc tttgctctga tggatccccc gttcgagaac gcgcgcagca ccaccgcgat
     1861 gcggttgatc gatctcgcgg tgaagcgtgg tttcaacgtc aacgtgttcg cgtatgaagg
     1921 cgcggtctat ctgccttttg cgaaacaggc gcaacatccg aatgcggtgc acggccgcag
     1981 cttggaagaa gagaaccacc cgctgccgcg cgcctggatc gccgcgctca tgaaagaggc
     2041 cgcagccaag ggcacgaaag tggactggat caattgcggg ctgtgtgtcg acgagcgcgg
     2101 cgcaggcgaa accatcgagg gtgtgcgccg gggcagtccc gtggatttct ggaagttcgc
     2161 cgaaagctcc gacaacacgc tggtcatcgg taccaaatag gaggccatca tgaaagtgct
     2221 gaatattgtc gaggccgcct atcgcgccac tctggaggaa caggacgatc ccatcatctg
     2281 gatctgctcg gcgatgaagg gcgccaacgc cgacatcgac gtgctcttgc gcggtaacgc
     2341 agtgaactat gcgatcaagg cgcaggacgc aagcggctta tcgttcggcg ggcaggcgca
     2401 aactcagcca cccaggatcg ccgacgatct ggtccggcta aagggcaagg gcgtgaaaat
     2461 ctacgtggtc gacgatgctc tgcgcgaacg cggcatcgag cctggcgagt tgatcgacgg
     2521 actggacttc gttccccgca gcaagctcgc caagctcttc ggtgggtacg atcaagtgtg
     2581 gcattggtaa gtcaggcgcc gtcgctttta tgacgcggac atcaacgaca tctgtcgcgc
     2641 gtggtggcga gcacgcggct tcgcgcgagg ttgctcatcg ggggcgtcat tctgtcaatc
     2701 gccccgcgcg gccgcttcgc gcagcttgtc cttgcggctc ttgcgccggc ccttgacgcc
     2761 gccttcgggc ggcgacgccg gcagcgcgct ctcgcgcggc tcgaagccgg cgatgcgctc
     2821 gcgcggtacg cgccggccct ggcgcttctc gatcagccgg aagtgcgcct cggtcgccgt
     2881 gctgacgaag ctgacggcca gtccgttggc gccggcgcgc gcggtgcggc cgatgcggtg
     2941 caggtagtcg accgccgagc gcggcaggtc gtagttgacg accaccggca gccccggtac
     3001 gtgcagcccg cgtgcggcga ggtcggtggc gagcaccacg cgcagcgcgc tgctgttcag
     3061 atcggcgagc acacgctggc gcgcgccttg gctgagctcg ccgtgcaagg ccgcggcgga
     3121 gaggccggcg cggcgcagct tctcggcaac gtgctcgctg gcgtagcgcg tcgcgacgaa
     3181 gaccagcaca cgctgccagc ggccctgtgc gatcaggtgg cgcagcagcg gcgtgcgctg
     3241 cgccgcgtcg acctccacgg cccgctgctc gatgtcgggc acgccggcgc ccgggccggc
     3301 cgcgacgtct atccgcagcg gctcgcgcag cagcgccgcc gccagcgcct gcacggccga
     3361 cgggaaggtg gccgagaaga acaggctctg ccggcgcgcc ggtagcagcg ccgttacgcg
     3421 ggcgaactca tcggcgaagc cctcgtcgag cagccggtca gcttcgtcga gcaccagcag
     3481 gccgacgtcg ccgagcccga gcgcatgccg ctcggctaga tcgagcagcc ggcccggcgt
     3541 cgcgacgatc acctcggcgc cgccgcgcag cgcgagcatc tgcgggttga tcgcgacgcc
     3601 gccgaagacg acggccacct tgagcggcgc ggccaggtgg cgggccagcg caccgatcgt
     3661 ctcgccgacc tgcacggcga gctcgcgtgt gggcaccagc accagggcgt cggtgcgccg
     3721 ccgggcgggg cgcgggccgt tgtccaggcg ctgcagcagc gccagcgcga acgcggccgt
     3781 cttgcccgag ccggtgtgcg cgcagccgac cagatcgcgg ccctgcagcg ccgccgggat
     3841 cgtcgccacc tggatcggcg tgggccgatc gtagccgcgc tcggcgacgg cgcgcaccag
     3901 ggccggcgac aggcccagcg cggcgaaagg tgaactcaca agcgcctcac tcataagcgc
     3961 ctcaccttga ccgaacggcc cttgaccttg ccgtcgttca ggccgcggca ggcccgctcg
     4021 gcgatgtcgc gctgcacggc gacgtaggtg gagaagtcgt tgacgtcgat cttgccgatc
     4081 gcggcggcgt cgaggccgag gtccttggtg agagcgccca gcacgtcccc tgggcggatc
     4141 ttctccttgc gccccccgag gatctgcaga gtgaccatcg gcgggcgcag cggctcgccg
     4201 ggcgcggcgt gctgggcgcc cgaggcctgc agttcggcca gcggatgcca cacgctttcg
     4261 ctgccctgca tctcgtcgat gcggccgacg cggcccatct cgtcgacgct ggccaggttg
     4321 aacaccagcc cggattcacc cgcccggccg gtgcggccgg cccggtgcgt gtgcagctgc
     4381 gcgtcgggcg tgatctcgac gttgatcacc gcctccagcc gcgcgatgtc cagcccgcgc
     4441 gcggcgacgt cggtggccac cagcacgctg cagctgcggt tggcaaagcg caccagcacc
     4501 tggtcgcgct cgcgctgctc gaggtcgccg tgcagttcga gcgcgacgat gccctgggcc
     4561 tgcagcaccg cgacgagatc gcggcactgc tgtttggtgt tgcaaaaggc cagcgtgctc
     4621 tcggggcgaa agtggttcag cagcaggccg acggcatgca gccgctcgct ctccgtcact
     4681 tcgtagaagc gctggtggat caggctcgcg gcatgccgct cgttgagcct gatctcctgc
     4741 ggctcgcgca ggtagcgggc cgcgagccgg gccacgtcgt cggggtaggt ggccgagaac
     4801 agcagcgtct ggcgctgcga cgtcggcggg cagtgctgcg cgatggcggc gatgtcttcg
     4861 gcgaagccca tgtcgagcat gcggtcggct tcgtcgagca ccagcgtgtt cagcgcgtcg
     4921 agcaccagcg actcgcgcac gacgtgatcg aggatgcgcc cgggcgtgcc gaccaccacg
     4981 tgtgcgccgt gtgccaggct ggccagctgc ggccgcatcg tcgcgccgcc gcacaacgtc
     5041 agcaccttga cgttgtcctc ggcgcgcgcg agacggcgga tctcctgcgt cacctgctcg
     5101 gccagctcgc gggtggggca cagcaccaga gcctgcacag cgaagcgccg cgggttcagg
     5161 ttcgccagca gcggcagcgc gaaggcggcc gtcttgccgc tgccggtctt ggcctgtgcg
     5221 atcaggtctt tgcccgcaag cgcgagcggc aggctggcgg cctggatcgg ggtcatgtgc
     5281 tcgtagccca ggcgctggag gttctccagc acgtggcttg cgaggggaag agtcgaaaag
     5341 gcggggccgg tgcgcggcgc gacgtcggaa gtggcggtca tggtcgattg tcaaccagcg
     5401 ccgcctctgg ggctctcagc gcgccacgcg ggcccgccag tgctcggccg gcagcgtcag
     5461 ctcgagcaga tcgcgctgcg cgtggctggc gcccagtcgc tgcgcggtcg cgcagcggcc
     5521 cccgtcgcct cggccgcgtc gccggccgca aaggccagca gatgtggtgc tcaagcagcg
     5581 aaaggcgaag cggccgccgg gaggctgcca gccataaact gggggggctg cgacctgcag
     5641 caagagttga tctgcaacgc agcggttgct gtccgtggcc atcgaggccg gtaaccacgg
     5701 tctcggacac cttgcagaca ttcggcaaga aaacggcacg tcgccctcaa tgaccggtat
     5761 ccaagggact gctgaccctg cccgtagctc aggcgattcg ggctgcgagt gaccgcttgc
     5821 ggacggcatc ttgcacatcg cccggtcaca cgtcgttgcc gatcgacata ctgcattact
     5881 tcagctttgt gcggcacgcc taatttgcat ttaacggccg aatctcttcc gctaggcacg
     5941 gagtaggcga tgaacacgtc cgcgatgata tcgccccgtt tgcatcgtta tctgctgcgc
     6001 tttcggtcct tgttccatga agcgcgcggc tacagcttcc ca
//
LOCUS       DASUCY010000588         2645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_687968, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000588 DASUCY010000000
VERSION     DASUCY010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687968"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..175)
                     /locus_tag="VFV25_11630"
     CDS             complement(<1..175)
                     /locus_tag="VFV25_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HEY1394020.1"
                     /translation="MKFLIRAGLFVLALAGAAGTALAQQPQIVIKFSHVVAPDTPKGR
                     GADYFKKLAEERTK"
     gene            complement(220..1413)
                     /locus_tag="VFV25_11635"
     CDS             complement(220..1413)
                     /locus_tag="VFV25_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY1394021.1"
                     /translation="MRPLDGVTVVTLEHAIAAPFCTRQLADLGARVIKVERPGVGDFA
                     RAYDERVRGLASHFVWTNRSKESLTLDVKHEAAQQILERLLSQADVLVQNLAPGAAAR
                     LGLSFEALHDRHPRLIVCDISGYGDDPARPGPYRDKKAYDLLIQSESGFLSITGSPDE
                     PAKAGCSIADIAAGMYAYTNVLAALIERGKTGRGRRIDVSMLESMVEWMGYPLYYSFD
                     GATPPPRAGAAHATIYPYGPFPTGDGHTVMLGLQNEREWQVFCQKVLQQPELIADERF
                     STNSRRTAARQALREIIVQAFAGLTADQVVVRLDEAQIANARMNNMREVWEHPQLKAR
                     RRWVQVDTPSGTVPALLPPGTPDEPGASRMDAVPALGQHTEAILAELGYEPADIASLR
                     EKRTI"
     gene            complement(1425..2588)
                     /locus_tag="VFV25_11640"
     CDS             complement(1425..2588)
                     /locus_tag="VFV25_11640"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY1394022.1"
                     /translation="MLKTACDRYQDIRDAVRALCAEFPDEYHRKIDEQRAYPEAFVDA
                     LTRAGWLAAMIPQDYGGSGLGLAEATVIMEEVNRAGGNSGACHGQMYNMGTLLRHGSQ
                     AQKRLYLPKIASGEWRLQSMGVTEPTTGTDTTRIKTPAVKKGDRYVVNGQKVWISRVQ
                     HSDWMILLARTTPLAEVEKKSEGMSIFMVDLRQAIGAGMTVRPIPNMVNHETNELFFE
                     NLEIPAENLIGEEGQGFKYILDGLNAERTLIAAECIGDGYWFVDRVTKYVGERVVFGR
                     PIGQNQGVQFPIAESYIEVEAANLMRWKACELFDAHQPCGAQANMAKYLAAKASWEAA
                     NACIQFHGGFGFATEYDVERKFRETRLYQVAPISTNLILSYVAEHVLGLPRSF"
BASE COUNT          377 a          865 c          927 g          476 t
ORIGIN      
        1 ccttggtgcg ctcctcggcc agcttcttga agtagtcggc gccccggccc ttgggcgtgt
       61 cgggcgctac gacgtggctg aacttgatga cgatctgcgg ctgctgggcc agtgcagtgc
      121 cggcggcgcc cgccagcgcc aagacaaaga gtccagcgcg aatgaggaat ttcatgtctt
      181 gtctccgtgg tggcgttgcc actgtgttgt cgaaacaaac tagatagtcc gcttctcgcg
      241 caggctggcg atgtcggccg gctcatagcc cagctcggcc aggatggcct cggtgtgctg
      301 gcccagggcc ggcaccgcat ccatgcgcga tgcgccgggc tcgtcgggcg tgccaggcgg
      361 cagcagtgcg ggcaccgtcc cgctcggcgt gtcgacctgc acccagcgcc ggcgcgcctt
      421 gagctgggga tgctcccaga cctcgcgcat gttgttcatt cgtgcgttgg cgatctgcgc
      481 ctcgtccagc cgcaccacca cctggtcggc cgtgaggccg gcgaaggcct gcacgatgat
      541 ctcgcgcagc gcctggcgcg ccgccgtgcg ccggctgttg gtggagaagc gctcgtcggc
      601 gatcagctcg ggctgctgca gcaccttctg gcagaacacc tgccattcgc gctcgttctg
      661 caggcccagc atcacggtgt ggccgtcgcc ggtcgggaag gggccatagg ggtagatggt
      721 ggcgtgcgcg gcgccggcgc gcggcggcgg ggtggcgccg tcgaaggagt agtacagcgg
      781 gtagcccatc cactccacca tgctctccag catcgacacg tcgatgcgcc gcccgcggcc
      841 ggtcttgccc cgctcgatca gcgcggccag cacattggtg taggcataca tgccggcggc
      901 gatgtcggcg atcgagcagc cggccttggc gggctcgtcg ggcgagccgg tgatggagag
      961 gaagcccgat tcgctctgga tcaagagatc gtaggccttc ttgtcgcgat acggcccggg
     1021 ccgcgcggga tcgtcgccat agcccgaaat gtcgcagacg atgaggcgcg ggtgcctgtc
     1081 gtgcaaggcc tcgaaggaca ggccgagccg tgccgcggcg ccgggcgcga ggttttgcac
     1141 cagcacgtcg gcctgggaca gcaggcgctc gaggatctgc tgcgctgctt cgtgcttcac
     1201 gtccagcgtc aggctctcct tggagcgatt ggtccacacg aaatgcgagg ccaggccgcg
     1261 cacgcgctcg tcgtaggcac gcgcgaaatc gcccacgccg ggccgctcga ccttgatgac
     1321 gcgcgcgccc aggtcggcca gctggcgcgt gcagaagggc gcggcgatgg cgtgctcgag
     1381 cgtgacgacc gtgacaccgt ccaggggtct catcggcggc ctcctcaaaa ggagcgtggc
     1441 aggccgagca catgctcggc cacgtagctc aggatcaggt tggtcgagat cggcgccacc
     1501 tggtagagcc gcgtctcgcg gaacttgcgc tcgacgtcgt attcggtggc gaagccgaag
     1561 ccgccgtgga actggatgca ggcattggcc gcctcccagc tcgccttggc cgccaaatac
     1621 ttggccatgt tggcctgggc gccgcagggt tggtgggcgt cgaacagctc gcaggccttc
     1681 cagcgcatca ggttggcggc ctcgacctcg atgtagctct cggcgatcgg gaactgcacg
     1741 ccctggttct ggccgatggg ccggccgaac accacgcgct cgcccacgta cttcgtgacg
     1801 cggtcgacga accagtagcc gtcgccgatg cattcggccg cgatcagcgt gcgctcggcg
     1861 ttcaggccat cgaggatgta cttgaagccc tggccctcct cgccgatcag gttctcggcc
     1921 gggatctcga ggttctcaaa gaagagctcg ttggtctcgt ggttgaccat gttggggatg
     1981 ggccgcaccg tcatccccgc cccgatggcc tggcgcaggt cgaccatgaa gatcgacatg
     2041 ccctcggact tcttctccac ctcggccagc ggcgtcgtgc gagccagcag gatcatccag
     2101 tcggagtgct gcacgcgcga gatccacacc ttctggccgt tgacgacgta gcggtcgccc
     2161 ttcttcaccg cgggggtctt gatcctggtg gtgtcggtgc cggtggttgg ctcggtcacg
     2221 cccatcgatt gcagccgcca ttcgccgctg gcgatcttgg gcaggtagag gcgcttctgc
     2281 gcctgcgagc catggcgcag cagcgtgccc atgttgtaca tctggccgtg gcaggcgcct
     2341 gagttgccgc cggcgcggtt gacctcttcc atgatcaccg tggcctcggc caggcccagg
     2401 cccgagccgc cgtagtcctg cgggatcatc gcggccagcc agccggcgcg ggtcagcgca
     2461 tcgacgaagg cctcgggata ggcacgctgc tcgtcgatct tgcggtggta ttcgtcgggg
     2521 aactcggcgc acagcgcgcg cacggcgtcg cggatgtcct ggtagcggtc gcaggcggtc
     2581 ttcagcatgg tggggggtgt tgttgtctgc tgctgcagct caggcgatct cggcgctgcc
     2641 ctgca
//
LOCUS       DASUCY010000589         2385 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1427551, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000589 DASUCY010000000
VERSION     DASUCY010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1427551"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1529
                     /locus_tag="VFV25_11645"
     CDS             <1..1529
                     /locus_tag="VFV25_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEY1394023.1"
                     /translation="DRDFIEKRASNFEALRENVKGYSPEAMAPICGIPAQTLRDVARE
                     FARAKGAMILWGMGVSQHVHGTDNARCLIALATVTGQIGKPGSGLHPLRGQNNVQGAS
                     DAGLIPMMFPNYQRVDDAGAHQWFENFWGMPLDDQPGYTVVEIMDKALAPDSDPHKIR
                     GMYIMGENPAMSDPDLHHARHALASLEHLVVQDIFMTETAWLADVVLPATAWPEKTGT
                     VSNTDRMVQLGKQAITPPGEAKPDLWIIQQIAQRIGPHAPHFVSSLPPEGAVPALGQP
                     GGGGMGLSWNYEGEHAGVAAVYEEMRQAMHAAIAGITWQRLERESSVTYPCLSADDPG
                     QPTVFIDRFPTDDGRVKLVPADIIPANERPDADYPFVLITGRQLEHWHTGSMTRRATV
                     LDAIEPMATASLCGADLAALGLQAGDVITVQSRRGEVAIHVRRDDGTPQGAVFIPFAY
                     YEAAANLMTNAALDPFGKIPEFKYCAVAVRRGGQPAVAAGYGTGGAQPGPRTPAAVSA
                     "
     gene            complement(1526..1879)
                     /locus_tag="VFV25_11650"
                     /pseudo
     CDS             complement(1526..1879)
                     /locus_tag="VFV25_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831926.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(2062..>2385)
                     /locus_tag="VFV25_11655"
     CDS             complement(2062..>2385)
                     /locus_tag="VFV25_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEY1394024.1"
                     /translation="GKLPDLNLGTANGESCDPALAQTLLGIGEAATAYTAVLNGRFKG
                     GHITRQYGQPGQNVHAIQLEIAQCSYMQEQLPFDYLPEPAARLQPHLRAMLEAVLRFA
                     ETRGR"
BASE COUNT          393 a          822 c          805 g          365 t
ORIGIN      
        1 ctgaccgcga tttcattgaa aagcgtgcca gcaactttga agcgctgcgt gaaaacgtca
       61 agggctacag ccccgaagcc atggcaccta tctgcggcat tccggcgcaa acgctgcgcg
      121 acgtcgcgcg tgagtttgcc cgggccaagg gcgcgatgat tctctggggc atgggcgtga
      181 gccagcatgt gcacggcacc gacaacgcgc gctgcctgat tgcgctggcc acggtgaccg
      241 ggcagatagg caaacccggc tcgggcctgc atcccctgcg cggccagaac aatgtgcagg
      301 gcgccagcga cgcgggcctc attcccatga tgtttcccaa ctaccagcgc gtggacgacg
      361 ccggcgcgca ccagtggttt gaaaacttct ggggcatgcc gctggacgac cagcccggct
      421 ataccgtggt ggaaatcatg gacaaggcac tcgcccccga cagcgacccg cacaagattc
      481 gcggcatgta catcatgggc gagaaccccg ccatgagcga ccccgacctg caccatgcac
      541 gccatgcgct ggccagcctg gagcacctgg tggtgcagga catcttcatg accgaaaccg
      601 cctggctggc cgatgtggtg ctgcctgcca ccgcctggcc cgagaaaacc ggcacggtga
      661 gcaacaccga ccgcatggtg cagctgggca agcaggccat cacgccgccc ggcgaggcca
      721 agcccgatct ctggatcatc cagcagatcg cgcaacgcat aggcccccac gctccgcact
      781 tcgtgtcgtc gctgcccccc gagggggccg tccccgcctt gggtcagccc ggcggcggag
      841 gcatgggcct ctcgtggaac tatgaaggtg agcatgcggg cgtcgcggcc gtgtatgagg
      901 aaatgcgcca ggccatgcat gccgcgattg cgggcatcac ctggcaacgg ctggagcgcg
      961 aatccagtgt gacctacccc tgcctgagtg ccgatgatcc gggccagccc accgtcttta
     1021 ttgaccgctt tcccacggac gatggccgcg tgaagctggt gccggccgac atcattccgg
     1081 ccaacgagcg tcccgacgcc gactacccgt tcgtgctgat caccgggcgg cagctggaac
     1141 actggcacac cggcagcatg acgcgccgcg ccacggtgct cgatgccatc gaacccatgg
     1201 cgacggcctc gctgtgcggt gccgacctgg cggcgctggg gctgcaggcg ggcgacgtga
     1261 tcacggtgca gtcccgccgc ggcgaggtcg ccattcatgt gcgccgcgat gacggcacgc
     1321 cgcagggcgc cgtcttcatc ccctttgcct attacgaagc ggcggccaac ctgatgacca
     1381 atgccgcgct ggacccgttc ggaaagatcc cggagttcaa gtactgcgcc gtcgctgtcc
     1441 ggcgtggcgg ccagcccgcc gtggcggcgg gctatggcac gggcggcgcg cagcccggac
     1501 cccggacccc ggccgcagtg agcgcctagg gcgccaggcc gagcacgtcc ctgaaggcct
     1561 gcaggaacag cgcgttgtcg ccggcgctgc cgatcgaaac gcggatgaaa tgcccgtagc
     1621 cggcttcctt ccagggcttg acgatgacac cgcgcgccag cagcccttcg gcgatgggcc
     1681 cattgggacg ctgcaggttc aggaacagga agttggccgc cgagggcgcc atcaccacgc
     1741 ctagtgcgcg caactgaccg gcgagcgcgt cgcgttgtgc cacggtttca cgcacgctca
     1801 tctccatgtg gcccgggtcg ccccaggcgg ccagcgcggc cgtctgcgcg gccaggttca
     1861 cattgaacgg cgtgcgcaag gtgcagggcc gccgccacgt cgccggtgct ggcggggcgc
     1921 agcagcgcga gcggacggac cggcgcggca ccgcttcagt cggcctggta acgcgcatca
     1981 acggcgccgc ctcagcgtac cgcttcggcg ccgagcgcgg cgacgacttc gccgatcagt
     2041 ggaccgcttg ggctgcccat gtcaacgccc gcgggtttca gcgaacctga gcaccgcttc
     2101 gagcatggcg cgcaggtgcg gctgcagccg tgccgccggc tcgggcaggt agtcgaacgg
     2161 cagttgctcc tgcatgtagc tgcactgggc gatctcgagc tggatcgcat gcacgttctg
     2221 cccgggctgg ccgtactggc gcgtgatgtg accgcccttg aagcggccat tgagcacggc
     2281 ggtgtaggcc gttgccgcct cgccgatgcc cagcagcgtt tgcgccagcg ccggatcgca
     2341 gctctcgccg ttggcggtgc cgaggttgag gtcgggcagc ttgcc
//
LOCUS       DASUCY010000590         2548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1186245, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000590 DASUCY010000000
VERSION     DASUCY010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186245"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1779)
                     /gene="rpoD"
                     /locus_tag="VFV25_11660"
     CDS             complement(<1..1779)
                     /gene="rpoD"
                     /locus_tag="VFV25_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEY1394025.1"
                     /translation="MTAKKPAPAQAAKAAAKPAAKTAAGKTAAAKPAAKAGKAAELEA
                     KKPATTAKTAVGKPTAKAEETKGKRGRKPKAETASKKTELVADEDFSDVEADFAEAEG
                     LPAEGSDDKPKAKPLRMKVSRAKERALMREFGLDETALTEEEVNKRRQELKTLIKMGK
                     TRGFLTHQEINDHLPEKLVEAEILEAIVSMLNDMGIAVYEQAPDAATLLIAGATTSTA
                     TEEEAEEEAEAALSTVDSEFGRTTDPVRMYMREMGTVELLTREGEIEIAKRIEGGLQA
                     MMLAISESPTTIAEILLLSDKMRAGELPISDVVDGFVSDDEADDYVAEEDFDEFDEEE
                     DDDGNGGSKALTKKLEELKAAALVRFDELRTHFERMRRAYEKEGYKSVAYNKAQLAVS
                     AEITSIRFTVKTIERLCQILRSQVDDVRRYERELRKIVVDKCGMPQEHFIKTFPPNAL
                     NLKWAEKEAAAGKPYSAVIGRNLPPIQDLQQKLVDLQARAVVPIEDLKLINKKMNQGE
                     KASRDAKKEMIEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKF
                     STYATWWIRQAITRSIADQARTIRIPV"
     gene            complement(2129..>2548)
                     /locus_tag="VFV25_11665"
     CDS             complement(2129..>2548)
                     /locus_tag="VFV25_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEY1394026.1"
                     /translation="RATWLLLHRCESWDGLSAEVQALLAEQPAPYGAAFALIERQLHE
                     HGPLGFDALAAELERNDPSVAGLATRVAQLHGLSPEIDVAAELRIVADRLQLQSVEEE
                     LQLLFESGALSEEASRRGKELIQRRAQLKSQAGAAGS"
BASE COUNT          359 a          802 c          853 g          534 t
ORIGIN      
        1 caccggaatg cggatggtgc gcgcctggtc ggcgatcgag cgcgtgatgg cctggcggat
       61 ccaccacgtg gcgtaggtcg agaacttgta gccgcggcgg tattcgaact tgtccaccgc
      121 cttcatcagg ccgatgttgc cctcctgaat caggtcgagg aactgcaggc cgcggttggt
      181 gtacttcttg gcgatcgaga tcacgaggcg caggttggcc tcgatcatct ccttcttagc
      241 gtcgcgcgag gccttctcgc cctggttcat cttcttgttg atgagcttca ggtcttcgat
      301 gggcaccacg gcgcgggcct gcaggtccac cagcttttgc tgcaggtctt gaatgggcgg
      361 caggttgcgt ccgatcacgg cgctgtaggg cttgccggcg gcggcttcct tctcggccca
      421 cttgaggttc agcgcattgg gcgggaaggt cttgatgaag tgctcctgcg gcatgccgca
      481 cttgtcgacc acgatcttgc gcagctcgcg ctcgtagcgg cgcacatcgt cgacctgcga
      541 gcgcaggatc tggcacaggc gctcgatggt cttgaccgtg aagcggatgc tggtgatctc
      601 ggcgctgacc gcgagctgag ccttgttgta ggccaccgac ttgtagcctt ccttctcgta
      661 ggcgcggcgc atgcgctcga aatgggtgcg cagctcgtcg aagcgcacca gggcggcggc
      721 cttgagctct tcgagcttct tggtgagcgc cttggagccg ccgttgccgt cgtcatcctc
      781 ttcttcgtcg aactcgtcga agtcttcttc ggcgacatag tcgtcagcct cgtcgtcgga
      841 gacgaagccg tcgaccacat cggagatcgg tagctcgccg gcgcgcatct tgtcggacag
      901 cagcaggatt tcggcgatcg tggtcggcga ttcggagatc gccaacatca tggcctgcaa
      961 gccgccctcg atgcgcttgg cgatctcgat ctcgccttcg cgggtcagca gctccaccgt
     1021 gcccatctcg cgcatgtaca tgcgcaccgg atcggtggtg cggccgaact cggaatccac
     1081 cgtggacagg gcggcctcgg cttcttcttc ggcctcttcc tcggtggcgg tcgaggtggt
     1141 ggcgccggcg atcagcaggg tcgcggcgtc gggcgcctgc tcgtagaccg cgatgcccat
     1201 gtcgttcagc atggacacga tggcctcgag gatctcggcc tcgaccaact tttcgggcag
     1261 gtggtcgttg atttcctggt gcgtcaggaa accacgtgtc ttgcccatct tgatgagggt
     1321 cttgagctcc tggcggcgct tgttgacttc ctcttcggtg agcgccgtct cgtccagccc
     1381 gaactcgcgc atcagcgcgc gctccttggc gcgcgacacc ttcatgcgca gcggcttggc
     1441 cttgggcttg tcgtcgctgc cctcggcggg cagtccctcg gcttcggcga aatcggcttc
     1501 gacgtcgctg aaatcctcgt cggcaaccag ctcggtcttc ttggaggccg tctcggcctt
     1561 gggcttgcgg ccgcgcttgc ccttggtctc ttcggccttg gcggtcggct tgccaactgc
     1621 ggtcttggct gtcgtggcag gcttcttggc ctcaagctcg gcggccttgc ctgccttggc
     1681 agcaggtttc gcggcggcgg tcttgcccgc agccgtcttg gcggcaggct tggcggcggc
     1741 cttggcggcc tgggcggggg cgggcttctt cgcggtcatg cgcttcctct agtgatttgg
     1801 ggctcgataa ctctcaggct tgcaagcagc ttggaccatg cttagatgcg tccagttcat
     1861 ggcagcgcaa ggccggcagg cctgcgcaac acagggacga aacaaagcac cgtgcacggc
     1921 aaagaccggc ttggggcaag caaagagcgg gcaagatcgc gttgacattt cagaggtgca
     1981 gcccttgcgg gtgaggttgg cctggtgtct gacccgtgtc gcgggtggcc ggggcccgga
     2041 ggcgctgctg tcactcttgc cgctgtaaca aactctcaat tatcggcgaa gatgcccggt
     2101 ccgtcaaagt tcggagcttt ttacggctct aagaaccggc cgcgccggcc tgggacttga
     2161 gctgggcccg gcgctggatc agctccttgc cgcgccgcga ggcttcctcg gacagcgcgc
     2221 ccgactcgaa cagaagctgc aactcctcct cgaccgactg caactgcagg cgatcggcga
     2281 cgatgcgcag ctcggcggcg acgtcgattt ccggcgagag cccatgtagc tgcgccaccc
     2341 tggtggccaa gcccgcgacg ctggggtcat tgcgctccaa ctcggcggcc agggcgtcga
     2401 agcccagcgg accatgctcg tgcagctggc gctcgatcag cgcgaaggcg gctccgtagg
     2461 gagcgggctg ttcggccagc agcgcctgga cttcggcgct caggccgtcc caggactcgc
     2521 agcggtgcag cagcagccag gtggcgcg
//
LOCUS       DASUCY010000591         2722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1089892, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000591 DASUCY010000000
VERSION     DASUCY010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1089892"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..880)
                     /locus_tag="VFV25_11670"
     CDS             complement(47..880)
                     /locus_tag="VFV25_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY1394027.1"
                     /translation="MKLQVLGREAYAYTGGKAFDANLPCAVFIHGALNDHSVWALQSR
                     YLAHHGHGVLAVDLPGHGRSAGPALTSVGALADWLIALLDAAGVQRAALVGHSMGSLI
                     ALEAASRLGERASRLVMVGTAYPMKVSAALLDTARTAPLKAIDMVTSFSIATLAAKPS
                     SPGPGSWLHGGARALMRKLQADYAREHGGANLFHLDFSACDAYTGGMEAAARLHCPAS
                     MILGEKDAMTMPRAAAELARATHAHITYLSAGHTLMGEAPDGVLNALKDSLRPQAPQP
                     S"
     gene            complement(877..2184)
                     /locus_tag="VFV25_11675"
     CDS             complement(877..2184)
                     /locus_tag="VFV25_11675"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HEY1394028.1"
                     /translation="MPGHADPGFDTLALHAGAAPDPATGARATPIHLSTSFVFQDSDH
                     AAALFNMERAGHVYSRISNPTTAVFEERMAALEGGVGAIATASGQAALHLAIATLAGA
                     GSHIVASSALYGGSHNLLTYTLARFGITTSFVKPGDIDGWRAAIRPDTRLLFGETLGN
                     PGLDVLDIPTLSALAHEHGLPLLVDSTFTTPYLMRPFEHGADLIYHSATKFLSGHGTV
                     IGGVLVDSGRFDWEASGRFPELTQPYAGFHDMVFTEESTVGAFLLRARREGLRDFGAC
                     MSPHTAWLILQGLETLSLRMEKHVSNARKIVDYLARHSFVESVAYPELESHPDHALAR
                     KLLPRGCGAVFSFNLKGDRAQGRRFIETLKIFSHLANVGDCRSLVIHPASTTHFRMDD
                     AALAQAGITQGTIRLSIGLEDADDLIDDLGRALKAAEKAGSGK"
     gene            2272..>2722
                     /locus_tag="VFV25_11680"
     CDS             2272..>2722
                     /locus_tag="VFV25_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEY1394029.1"
                     /translation="MKVSDILRVKGGTLFTVVPDQAMADAVKTMAEHDIGSLVVMEHG
                     DLVGMLTFREVIQAVVANGGTVGSTIVRTVMDDHPLTCTPETEIDEVRRMMLSRHARY
                     MPVMSQKTLMGVISFYDVAKAVVESQDFENRMLKAYIRDWPVEEEQGA"
BASE COUNT          446 a          847 c          967 g          462 t
ORIGIN      
        1 aagctcttga aggaccgagg gtcgagatcg ggtgaggtgg tggccgtcat gatggttgcg
       61 gcgcctgcgg gcgcaagctg tccttcaacg cattcaacac cccgtcgggc gcttcaccca
      121 tcagggtatg cccggcgctc aagtaagtga tgtgagcatg cgtggcccgg gccaactcgg
      181 cggcggccct gggcatcgtc atggcatcct tttcgccgag gatcatgctg gcggggcagt
      241 gcagcctggc tgcggcctcc atcccgcccg tgtaggcatc gcaggccgag aagtccaggt
      301 gaaagagatt ggcgccgccg tgctcacgcg cgtagtcggc ctgcagcttg cgcatcagcg
      361 cccgagcccc gccatgcagc cagcttcccg ggcccggcga cgagggcttg gccgccagcg
      421 tggcgatgga aaaggaggtc accatgtcga tcgccttcag cggcgctgtg cgcgccgtgt
      481 ccagcagtgc cgccgagacc ttcatcggat aggccgtgcc taccatgacg aggcggctgg
      541 cgcgctcgcc cagccggctc gcggcctcca gcgcgatcag cgagcccatg ctgtggccga
      601 ccagtgccgc gcgctgcacg ccggcggcgt cgagcagggc gatcagccaa tcggccagcg
      661 ctccgacgct cgtcaaggcc ggcccggcgc tgcggccatg gccgggcagg tcgacggcca
      721 gcacgccatg gccgtgatgc gccagatagc ggctctgcaa ggcccagaca ctgtggtcgt
      781 tcagcgcgcc gtggatgaag actgcgcagg gcaggttggc gtcgaaagct ttgccgcccg
      841 tgtaggcata agcctcgcgg ccgaggactt gcagcttcat ttgccgctcc cggctttctc
      901 tgcggccttg agcgctcgcc cgaggtcgtc gatcaggtcg tcggcatctt ccagtccgat
      961 cgacaagcgt atcgttcctt gcgtgatgcc ggcctgtgcg agggccgcgt cgtccatgcg
     1021 gaaatgcgtg gtcgaggcag ggtggatcac gagcgagcgg cagtcgccca cattggccag
     1081 gtgcgagaag atcttcagcg tttcgatgaa gcggcgtccc tgggcccggt cgcccttgag
     1141 gttgaagctg aacacggcac cgcagccgcg cggcagcagc ttcctggcga gcgcgtggtc
     1201 cgggtggctc tcgagttccg gataggcgac gctctcgacg aaggaatggc gggccaggta
     1261 gtcgacgatc ttgcgtgcat tggaaacatg cttttccata cgcaaggaaa gtgtctccag
     1321 gccctgcagg atcagccagg ccgtgtgcgg gctcatgcag gcgccgaagt cgcgcaggcc
     1381 ctctcggcgc gcgcgcaaga ggaaggcgcc gaccgtgctc tcttccgtga acaccatgtc
     1441 gtggaagccg gcatagggct gggtcagttc tgggaagcgg ccggaagcct cccagtcgaa
     1501 gcggccgctg tcgaccagca cgccgccgat cacggtgccg tggccggaca ggaacttggt
     1561 ggccgagtga tagatgaggt ccgcgccatg ctcgaagggc cgcatcagat agggggtcgt
     1621 gaaggtcgag tcgaccagca gcggcaggcc gtgctcatgg gcgagggcgc tgagcgtcgg
     1681 gatgtcgagc acgtcaagcc ccgggttgcc cagggtctcg ccgaacagca gccgggtgtc
     1741 ggggcggatc gcggcgcgcc agccgtcgat gtcgccgggt ttcacgaagc tggtcgtgat
     1801 gccgaagcgc gccagcgtgt aggtcagtag gttgtgcgag cccccgtaga gcgccgaact
     1861 ggccacgatg tgcgagcctg cacccgccag cgtggcgatc gccagatgca gagcggcctg
     1921 gccgctggcc gtcgcgatgg cgccgacgcc gccctcgagc gctgccatgc gttcttcgaa
     1981 cacggctgtg gtcggattgg agatgcgcga atagacgtga cctgcgcgct ccatgttgaa
     2041 cagggccgcg gcgtggtcgc tgtcctggaa cacgaaggag gtcgacaagt ggatgggcgt
     2101 ggcccgcgca ccggtggcgg ggtccggggc ggcgcccgcg tgcagcgcaa gcgtgtcgaa
     2161 gccggggtcg gcgtggcctg gcatatgttg actcctctga gctttacctg tggtgttcgc
     2221 cgggcattgt gggctatatt gctttcgagt ttggccacca ggagaccctc catgaaagtc
     2281 agcgacatcc tgcgcgtcaa gggcggcacg ctgttcactg tggtgcccga tcaagccatg
     2341 gccgatgccg tcaagaccat ggccgagcac gacatcggct cgctcgtggt catggagcat
     2401 ggcgacctgg tggggatgct gaccttccgc gaagtgatcc aggccgtggt cgccaatggc
     2461 ggcacggtgg gttccaccat cgtgcgaacg gtgatggacg atcatccgct cacctgcacg
     2521 cccgagaccg agatcgacga ggtgcgccgc atgatgctct cccgtcacgc gcgctacatg
     2581 cccgtgatga gccagaagac gctgatgggc gtgatctcct tctacgatgt cgccaaggcc
     2641 gtggtcgaga gccaggactt tgaaaaccgc atgctcaagg cctatatccg cgactggccg
     2701 gtcgaggagg agcagggcgc gc
//
LOCUS       DASUCY010000592         2311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_335003, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000592 DASUCY010000000
VERSION     DASUCY010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335003"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="VFV25_11685"
     CDS             complement(<1..713)
                     /locus_tag="VFV25_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY1394030.1"
                     /translation="MAGHSKWANIQHRKGRQDEKRGKIWTRVIREISVAARQGGGDPA
                     MNPRLRLAIDKAKAANMPADTVKKNIDKATGNLEGVNYEEIRYEGYGIAGAAIIVDCM
                     TDNRVRTVAEVRHAFSKYGGNLGTEGSVAFQFKHVGQLVFAPGTSEDKVMEVALEAGA
                     DDVVSDEYGAIEVLCAPADFEAVKQALEAAGLKPDVAEVTMRAENTVELSGEDAQRMQ
                     KLLDVIEDLDDVQDVFHNAV"
     gene            848..1360
                     /locus_tag="VFV25_11690"
     CDS             848..1360
                     /locus_tag="VFV25_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011549119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEY1394031.1"
                     /translation="MPDTPSVEELLALDLLTLRAHTERAGDKLEEEPLRIRLNASLAV
                     SETCTVRRGGALVAYAMLRPESANSWFVTGFNTHPAHRNSGVLFDLFVAFAEIVRRHG
                     IAELRSHVYKTNLLSMAFHRRLGFRVTRENAKGVEFFGTVADLMASPAFEKTARRLAN
                     AASARLDCAT"
     gene            complement(1353..>2311)
                     /locus_tag="VFV25_11695"
     CDS             complement(1353..>2311)
                     /locus_tag="VFV25_11695"
                     /inference="COORDINATES: protein motif:HMM:NF042682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6537 domain-containing protein"
                     /protein_id="HEY1394032.1"
                     /translation="AQNISQTLLGDTMPSNIVMLGACYQRGLIPLSEAALMRAIELNA
                     VAVAGNKAAFALGRLAVAAPEALRRLAGSRHTNQAADALDSEQGLIARRATFLAGYQD
                     AAYAQRYRALVERVRAAELGLGEPGQALKLTTAVARYYAKLLAIKDEYEVARLYTDGS
                     FEAALKEQFESWEHLSFHMAPPLLAKPRSDGRIDKIEFGAWWMRALKLLAPLRRLRGT
                     PLDIFGYTHERRMERQLIADYEGLVDELLASLKPGAGADKLELAVRLASLPEGIRGYG
                     HVKLANVASVKARWKDLLDRFHGRAADVPAVPVRMVERQVLK"
BASE COUNT          343 a          796 c          742 g          430 t
ORIGIN      
        1 actgcgttgt ggaatacgtc ctgcacgtcg tcgagatcct cgatgacgtc cagcagcttc
       61 tgcatgcgct gcgcgtcctc gccgctgagc tcgacggtgt tttcggcgcg catcgtcacc
      121 tcggccacgt cgggcttgag gccggccgcc tccagcgcct gcttcacggc ctcgaagtcg
      181 gccggcgcgc acagcacctc gatggcgccg tactcgtcgc tcaccacatc gtcggcgccg
      241 gcttccagcg ccacctccat caccttgtcc tcgctggtgc cgggcgcgaa caccaactgg
      301 cccacatgct tgaactggaa ggccaccgag ccttcggtgc cgaggttgcc gccgtacttg
      361 ctgaaggcat gccgcacctc ggccacggta cgcacccggt tgtcggtcat gcagtcgacg
      421 atgatggccg caccggctat gccatagccc tcgtagcgga tttcttcata gttcacgccc
      481 tcgaggttgc cggtggcctt gtcgatgttc ttcttgaccg tgtcggccgg catgttggcg
      541 gctttggcct tgtcgatggc caggcgcagc cgcggattca tcgcggggtc gcccccgccc
      601 tggcgcgccg ccacgctgat ttcgcggatc acgcgggtcc agatcttgcc ccgcttctcg
      661 tcctggcgcc ctttgcggtg ctggatattg gcccacttgg aatgaccggc catgctgtgc
      721 tttcgttcta ggtaatggct gtacgatggc cgcgatttta ccggggggaa tccatggccg
      781 aagccatccg gttcgccaag tgcagggaga tcatccccgc cggccgcccc atctgccaaa
      841 cgtggtcatg cccgatacgc cctccgtcga agaactgctc gccttggatc tgctcacgct
      901 acgcgctcac actgagcgtg ccggggacaa gctggaagag gagcctttgc gaatcaggct
      961 gaacgcctcc ttggctgtct cggaaacctg cacggtgcgg cgcggcggtg cgctcgtcgc
     1021 ctacgcgatg ctgcggcccg aatcagccaa cagctggttc gtcaccggct tcaacacaca
     1081 cccggcgcat cgcaactcgg gcgtgctgtt cgatctcttc gtcgcctttg cagagatcgt
     1141 gcgccgccac ggcattgccg agctgcgtag ccatgtctac aagaccaatc tgctgtcgat
     1201 ggcctttcat cgccggctgg gctttcgcgt gacgcgcgag aacgccaagg gcgtggaatt
     1261 cttcggcacg gtggccgatc tgatggctag ccccgctttc gagaagacgg ccaggcggct
     1321 tgcaaacgca gcctccgcaa ggctggactg cgctacttga gtacctgccg ctcgaccata
     1381 cgcaccggca ccgccggcac gtcggccgct cggccatgga agcgatcgag caggtccttc
     1441 cagcgcgcct tgacgctcgc cacgttggcc agcttcacgt gaccgtagcc gcgtatgcct
     1501 tcgggcaggc tggccaggcg cacggcgagc tccagcttgt cggcgcccgc gccgggcttc
     1561 agagacgcca gcagctcgtc gacgaggccc tcgtagtcgg cgatcagctg gcgctccatg
     1621 cggcgttcat gcgtgtagcc gaagatatcc agcggcgtgc cgcgcaggcg gcgcagcggg
     1681 gccagcagct tcagggcccg catccaccag gcgccgaact cgatcttgtc gatgcggccg
     1741 tcggagcgcg gcttggcgag cagcggcggc gccatgtgga agctcaaatg ctcccagctc
     1801 tcgaactgct ccttgagcgc cgcctcgaag ctgccgtccg tatagaggcg agccacttcg
     1861 tactcgtcct tgatcgccag cagcttggcg tagtagcgag ccaccgccgt ggtcagcttc
     1921 agagcctggc cgggctcgcc taagcccagt tcagcggcgc gcacgcgctc gaccagggcg
     1981 cggtaacgct gggcataggc ggcgtcctgg tagccggcca ggaaggtggc gcggcgcgcg
     2041 atcaggcctt gctcgctgtc gagcgcgtcg gccgcttgat tcgtgtgccg gctgcctgcc
     2101 aggcgccgca acgcctcggg cgcggccacc gcgagccggc ccagcgcgaa ggcggccttg
     2161 ttgccggcca ccgcgacggc attgagctcg atcgcgcgca tcagcgccgc ctcgctcaag
     2221 gggatcagcc cgcgctgata gcaggcgccc agcatcacga tgttggacgg catggtgtcg
     2281 ccgagcaggg tctgcgaaat gttctgcgcg t
//
LOCUS       DASUCY010000593         5087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_607698, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000593 DASUCY010000000
VERSION     DASUCY010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5087
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607698"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3101)
                     /gene="purL"
                     /locus_tag="VFV25_11700"
                     /gene_synonym="purI"
     CDS             complement(<1..3101)
                     /gene="purL"
                     /locus_tag="VFV25_11700"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEY1394033.1"
                     /translation="MTSSSSQILSFEGGNALSAFRAQALLPRLQAISGRVTAIHARHV
                     HWVWTDHGLDRPALDKLEALLTYGDPYAGPTEGELIVVTPRFGTVSPWASKATDIAHN
                     CGLAVHRMERVTEYRLTLKSGLLGAAKALTQEERQAAAVLLHDRMTESAAFERQAAAH
                     LFDEQPGLALEHVDVLGRGRAALEAANTEFGLALSDDEIDYLVSAFQGLKRNPSDVEL
                     MMFAQANSEHCRHKIFNAQFTIDGQAQPLSMFGMIRNTEKLAPRHTVVAYSDNAAVME
                     GGPAERWLPQSFTNAPLYGARQATAHVLMKVETHNHPTAISPHPGASTGAGGEIRDEG
                     ATGRGAKPKAGLTGFSVSNLLLPGTNEPWEQLRYGKPEHIASPLQIMTEGPLGGAAFN
                     NEFGRPNLGGYFRVYEQTVAGVRRGYHKPIMIAGGVGTIDAGQTHKLPFPAGTLLIQL
                     GGPGMRIGMGGGAASSMAAGANTAALDFDSVQRGNPEIQRRAQEVINHCWALGEANPV
                     LAIHDVGAGGISNAFPELVDGAHKGATFNIRKVPLEETGLAPKEIWCNESQERYVLAI
                     SPESLPIFTAMAERERCPFAVIGSATDDKELILEEGPGGERAIDMPMEVLLGKPPKMQ
                     RDVERVAREERPLDLTGVKLEQVAVDVLRHPTVASKRFLVTIGDRTVGGLNHRDQMVG
                     PWQVPVADCAVTLADYAGFRGEAMSMGERTPLAALDAPASGRMAVGEAITNLLAAPIE
                     LPRVKLSCNWMAACGEPGEDAALYDTVKAVGMELCPALGIGVPVGKDSLSMRTRWSEG
                     GESKQVTAPVSLIVSAFATLDEVRGTLTPQLQAGDTTLILIDLGQGRQRMGGSMLAQV
                     LNQFGDVVPDLDDAAQLKALVGAINKLRSEDKLLAYHDRSDGGLWAAACEMAFAGHLG
                     MSLNVDMLVTESDGIADSRAEYGDSKNWAQQVGERRAELTLKALFNEELGALIQVRTG
                     ERDAVMATLREFGLSRHSHVVGKPNDKGVVEVWRDAKAVFSAPLASLHQAWDEVSWRI
                     AQQR"
     gene            3212..4018
                     /gene="map"
                     /locus_tag="VFV25_11705"
     CDS             3212..4018
                     /gene="map"
                     /locus_tag="VFV25_11705"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEY1394034.1"
                     /translation="MTITIKTGADIDGMRVAGKLASEVLDYLTPHVKPGSTTEQLDKL
                     AHDYIVNVQQAVPAPLNYAPPGYTPYPKSICTSINHQVCHGIPNDRPLKNGDILNIDV
                     TVIKDGWHGDTSRMFVVGEGSIAAKRLCTLTYEAMWRGIVKVKPGIRLGDIGHSIQVF
                     AENQGFSVVREFCGHGIGRKFHEEPQVLHYGRPGTLEELVPGMTFTIEPMINAGRREI
                     KETGDGWTIVTKDRSLSAQWEHTVLVTETGYEVLTLSAGSPPPPAFVAAA"
     gene            4044..>5087
                     /locus_tag="VFV25_11710"
     CDS             4044..>5087
                     /locus_tag="VFV25_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEY1394035.1"
                     /translation="MSAVVVGEATPSALQVLRSRFRDGKEQLLGQFRDVRASAPAAAR
                     LIRGLAKHVDLSLQDLWDHAFMPSGAALLAVGGYGRGELFPYSDVDVLVLLPTPVHDG
                     NGRANEALKSAVEGFITACWDIGLEIGSSVRTVDECLAEAQGDVTIQTALLESRPVCG
                     SRRLWSEFRRASAAAMNPSSFLRAKTLEMRQRHTKYEDTPYSLEPNCKESPGGLRDLQ
                     TLIWVARAAGLGKTWGELAANGLATPFEVKQLQRNEGLLKLIRARLHVVAGRREDRLV
                     FDLQTAVAESFGYQATKAHRASEVLMRRYYWAAKAVTQLTQILLLNIEERINGSQGAR
                     MRPINEKFFDRGGM"
BASE COUNT          771 a         1751 c         1730 g          835 t
ORIGIN      
        1 cgctgctgcg cgatgcgcca gctcacctca tcccaggcct ggtgcagaga tgccagcggc
       61 gccgagaaca cggccttggc gtcgcgccac acctcgacca cacccttgtc attgggcttg
      121 ccgaccacgt ggctatgcct ggacaggccg aattcgcgca gcgtcgccat cacggcgtcg
      181 cgctcgcccg tgcgcacctg gatgagggcg cccagctcct cgttgaacag cgccttcagc
      241 gtcagctcgg cacggcgctc gccgacctgc tgcgcccagt tcttggaatc gccatattcg
      301 gcgcggctgt cggcgatgcc gtcgctctcg gtgaccagca tgtcgacgtt caggctcatg
      361 cccaggtggc cggcgaaggc catttcgcag gccgccgccc acaggccgcc gtcgctgcgg
      421 tcgtgatagg cgagcagttt gtcctcgctg cgcagcttgt tgatggcgcc gaccagcgcc
      481 ttcagctgag ctgcgtcatc gagatcgggc acaacatcgc caaactggtt cagcacctgg
      541 gccagcatcg agccgcccat gcgctgcctg ccctgcccca ggtcgataag gatcagcgtg
      601 gtgtcaccag cttgcaattg cggcgtcagc gtgccgcgca cctcgtccag cgtggcgaag
      661 gccgagacga tgagcgatac cggtgcggtg acctgcttgc tttcaccgcc ctcgctccag
      721 cgcgtacgca tcgacaagga atccttgccg accggcacgc cgatgcccag cgccgggcac
      781 agctccatgc cgaccgcctt gaccgtgtcg tacagcgccg catcctcgcc cggctcgccg
      841 caggcggcca tccagttgca gctgagcttc acgcgcggca gctcgatagg cgcggccagc
      901 aggttggtga tggcctcgcc caccgccatg cggcctgagg ccggtgcatc gagcgccgcc
      961 agcggcgtgc gctcgcccat gctcatcgcc tcgccgcgga agcccgcgta atcggccagc
     1021 gtcacggcgc aatcggccac cggcacctgc caggggccga ccatctggtc gcggtggttg
     1081 agcccgccga cggtgcgatc gccgatggtg acgagaaagc gcttggaggc cacggtggga
     1141 tggcgcagca cgtccacggc cacctgctcc agcttcacgc cggtgaggtc gagcggcctc
     1201 tcctcgcgcg ccacgcgctc gacgtcgcgc tgcatcttgg gcggcttgcc cagcagcacc
     1261 tccatcggca tgtcgatcgc gcgctcgccg cccgggcctt cttcgaggat cagctccttg
     1321 tcgtcggtgg cggagccgat cacggcgaac gggcagcgct cgcgctcggc catcgccgtg
     1381 aagataggca ggctttcggg cgagatggcc agcacgtagc gctcctggct ctcgttgcac
     1441 cagatctcct tgggcgccag gccggtctct tccagcggca ccttgcggat gttgaaggtc
     1501 gcgcccttgt gcgccccgtc caccagctcg ggaaaagcgt tgctgatgcc gccggccccc
     1561 acgtcatgga tcgcgaggac agggttggct tcacccagcg cccagcagtg gttgatgacc
     1621 tcctgcgcgc gccgctggat ctccgggttg ccgcgctgca ccgagtcgaa atcgagcgcg
     1681 gccgtgttgg cgccggccgc catcgagctg gccgcgccgc cgcccatgcc gatgcgcatg
     1741 ccggggccgc cgagctggat cagcagcgtg cctgcaggga acggcagctt gtgcgtctgc
     1801 ccggcatcga tggtgccgac tccgccggcg atcatgatgg gcttgtggta gccacggcgc
     1861 acgccggcca cggtctgctc gtagacgcgg aagtagccgc ccagattcgg tcggccaaac
     1921 tcattgttga aggccgcgcc gcccagcggg ccttcggtca tgatctgcaa ggggctcgcg
     1981 atgtgctcgg gcttgccgta gcgcagctgc tcccagggtt cattggtgcc gggcagcagc
     2041 aggttgctca ccgagaagcc ggtgagcccc gccttgggct tggcgccgcg gccggtggca
     2101 ccctcgtcgc ggatctcacc gccggcgccg gtagaagcgc ccgggtgagg agagatcgcc
     2161 gtggggtggt tgtgcgtctc caccttcatc agcacgtggg ccgtggcctg gcgggcgccg
     2221 tagagcgggg cattggtgaa gctctgcggc agccagcgct cggccggccc gccctccatg
     2281 accgctgcgt tgtcgctgta ggccaccacg gtgtgccggg gcgccagctt ctcggtgttg
     2341 cggatcatgc cgaacatcga cagcggctgc gcctggccat cgatggtgaa ctgcgcgttg
     2401 aagatcttgt ggcggcagtg ctcgctgttg gcctgggcga acatcatcag ctcgacatcg
     2461 ctggggttgc gcttgaggcc ctggaaggcg ctcaccaggt agtcgatctc gtcgtcgctg
     2521 agcgcgaggc cgaattctgt gttggccgcc tccagcgcgg cgcggccgcg gcccagcacg
     2581 tccacgtgct ccagggccaa gcccggctgc tcgtcgaaca gatgggcggc ggcctggcgc
     2641 tcgaaggccg cgctctcggt catgcggtcg tgcagcagca ccgccgcggc ctggcgctcc
     2701 tcctgagtca gtgccttggc cgcgcccagc aggccgctct tgagcgtgag gcggtattcg
     2761 gtgacccgct ccatgcggtg cacggccagc ccgcagttgt gggcgatgtc ggtggccttg
     2821 gaggcccatg gcgagacggt gccgaagcgc ggcgtcacga cgatcagctc gccctccgtc
     2881 ggtccggcat aggggtcgcc ataggtgagc agggcctcca gcttgtcgag ggccggcctg
     2941 tccaggccgt ggtcggtcca gacccaatgc acgtggcgtg catgaatcgc cgtgacgcgg
     3001 ccggatatcg cctgcagcct gggtagcagg gcctgggcgc ggaaagcgga cagggcgttg
     3061 ccgccctcga aggaaagaat ctgggagctg ctggaggtca cagggggctc gcttgggggc
     3121 tttgcagtgg ggcggcggga cgtgttccgg gttgggcgct ctatcccgcg aagcccgtga
     3181 ttttacgggc cgggtgagat aattccggcc catgactatc acgatcaaga ccggtgccga
     3241 tatcgacggc atgcgcgtcg ccggcaagct ggcctccgag gtgctggact acctcacccc
     3301 gcatgtgaag cccggctcca ccaccgagca gctggacaag ctggcgcacg actacatcgt
     3361 caacgtgcag caggccgtgc cggcgccgct caactacgcg ccgccgggct acacgcccta
     3421 tcccaagtcg atctgcacct cgatcaacca ccaggtctgc cacggcatcc cgaacgaccg
     3481 ccccctgaag aacggcgaca tcctcaacat cgatgtcacc gtgatcaagg acggctggca
     3541 tggcgacacg agccgcatgt tcgtggtcgg cgagggctcg atcgcggcca agcgcctgtg
     3601 caccctcacc tacgaggcga tgtggcgcgg catcgtcaag gtcaagcccg gcatacgcct
     3661 gggcgacatc ggccattcga tccaggtctt cgccgagaac cagggctttt ccgtcgtgcg
     3721 cgagttctgc ggccacggca tcggccgcaa gttccacgaa gagccgcagg tgctgcacta
     3781 cggccgtccc ggcacgctgg aagagctggt gcccggcatg accttcacga tcgagccgat
     3841 gatcaacgcc ggccggcgcg agatcaagga aaccggcgac ggctggacca tcgtcaccaa
     3901 ggaccgctcg ctgtcggcgc aatgggagca cacggtgctg gtcaccgaga ccggttatga
     3961 ggtgctgacc ctgtcggccg gcagcccgcc gccgccggct ttcgtcgccg cggcctgaag
     4021 ccgcggacgc gcgcgggatc gccatgtctg ctgtcgtcgt gggtgaagcc acgccctctg
     4081 cgctgcaggt tctgcgctcg cgatttcgcg acggcaagga acagctgctt gggcaattcc
     4141 gcgacgtgcg cgcctcggcg ccggccgctg cgcggctgat ccgcggcctg gccaagcatg
     4201 tcgacctgag cctgcaggat ctctgggacc atgccttcat gcccagcggc gccgcgctgc
     4261 tggccgtcgg cggctatggc cgcggggagc tgttccccta ctccgatgtc gacgtgctgg
     4321 tgctgctgcc cacgccggtg cacgacggta atggccgcgc caacgaggcc ctgaagtcgg
     4381 ccgtcgaggg cttcatcacc gcctgctggg acatcggcct ggaaatcggc tccagcgtgc
     4441 gcaccgtgga cgaatgcctg gccgaagccc agggcgacgt gacgatacag actgcgctgc
     4501 tggagtcgcg cccggtgtgc ggatcgcgcc gactgtggag cgagttccgg cgcgcctcgg
     4561 cggcggcaat gaatccctca tccttcctgc gcgccaagac gctggagatg cgccagcgcc
     4621 acacgaaata cgaggacacg ccctactccc tggagcccaa ctgcaaggag agcccggggg
     4681 gcttgcgcga tctgcagacc ctgatctggg tggcgcgcgc cgcgggcctc ggcaagacct
     4741 ggggcgagct ggccgccaat ggcctggcca cgcccttcga agtcaagcag ctgcagcgca
     4801 acgagggcct gctcaagctg atccgcgccc gcctgcatgt ggtggccggc cggcgcgagg
     4861 accgcctcgt gttcgacctg cagacggccg tggccgagag cttcggctac caggccacca
     4921 aggcacaccg cgcatcggaa gtgctgatgc gccgctacta ctgggcggcc aaggcggtga
     4981 cccagctgac ccagatcctg ctgctcaata tcgaggagcg catcaatggt tcgcagggcg
     5041 cgcggatgcg gccgatcaac gagaagttct tcgaccgcgg cggcatg
//
LOCUS       DASUCY010000594         2005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_158261, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000594 DASUCY010000000
VERSION     DASUCY010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158261"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VFV25_11715"
     CDS             <1..516
                     /locus_tag="VFV25_11715"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEY1394036.1"
                     /translation="LLGAMTIKGIATGPQLMASEPRLFWGLIASAWAGNLMLVLLSLP
                     LAGIWGRVLRLPYRLLVPVVVVASCLGCYSLKHSGSDVYMAAACAAVAYALHKLDCEA
                     APLLLGFVLAPATQDSLRHALLLSHGDWGVFLNHPLSAALLLGAVLVMMLMVLLPSSR
                     KGRGSVALEED"
     gene            540..>2005
                     /locus_tag="VFV25_11720"
     CDS             540..>2005
                     /locus_tag="VFV25_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEY1394037.1"
                     /translation="MSLAFDYASPYRSTRLPLFARNVVATSQPLAAQAGLRMLLKGGN
                     AVDAAIAAAAALTVVEPVSNGLGSDAFCILWDGQSLQGLNASGRAPQAWTREYFHAKY
                     GADALHPPKRGWDSVTVPGAVAGWVALSERHGRLPFADLLEPAIELAERGHAVSVVVQ
                     QKWAVAAEELAAQPGFREVFLPWGRAPQVGELFRNPGAAKALRLIAETKGAAYYQGEI
                     AAAAATHARQHGGAITAEDFAAYQAEWVAPIAQDYRGYTLHEIPPNGQGIAALIALGI
                     LSRFDLAALPPDSVEAQHLQIEAMKLAFADVYRYVAEPRSMELTPAQMLDAGYLAARA
                     SSIDMKRAQDFGAGNPAKGGTVYLTTADESGMMVSFIQSNYMGFGSGVVVPQFGLSMQ
                     NRGHAFSLAAAGPNVVAPGKRPFHTIIPAFLTRGGEPVMSYGVMGANMQPQGHVQTLV
                     RMLDYRQNPQAACDAPRWRYNAGLELNVEASMDPAVVQG"
BASE COUNT          285 a          698 c          685 g          337 t
ORIGIN      
        1 ctgctgggtg ccatgacgat caagggcatc gcgaccggcc cccagctcat ggcgagcgag
       61 ccccgcctgt tctggggcct catcgcatcg gcctgggcgg gcaacctgat gctggtgttg
      121 ctcagcctgc cgctggccgg catctggggc cgcgtgctgc gcctgcccta tcgcctcctc
      181 gttcccgtcg tcgtcgtggc ttcctgcctg ggctgctaca gcctcaagca cagcggttcc
      241 gacgtctata tggccgccgc ctgcgcggct gtggcctatg ccttgcacaa gctggactgc
      301 gaagcggcgc ccttgctgct gggcttcgtc ctcgcgccgg cgacgcagga cagcctgcgc
      361 cacgccttgt tgctgtcgca cggcgattgg ggcgtcttcc tcaaccaccc gctgtccgcg
      421 gccctgctgc tgggggcggt gctggtcatg atgctgatgg tgctgctgcc ctcgtcgaga
      481 aaagggcgcg gctcggtggc gctggaagaa gactgatact cgtcggacga ggggttgtca
      541 tgagcctagc tttcgactac gccagccctt atcgcagtac gcgcctgccg ctgttcgcgc
      601 gcaacgtggt cgccacctcg cagccgctag ctgcccaggc cgggctgcgc atgctgctca
      661 agggcggcaa cgcggtggac gcggccatcg cagcggcggc agcgctcacc gtggtcgagc
      721 cggtgagcaa tggcctcggc tcggatgcct tctgcatcct ttgggacggc cagtcgctgc
      781 aggggctcaa tgcctctggc cgcgcgccgc aggcttggac gcgcgagtac ttccacgcca
      841 agtacggcgc ggatgccctg cacccgccca agcgcggctg ggacagcgtg accgtgccgg
      901 gcgcggtggc cggctgggtg gccttgagcg agcgccatgg ccggctgcct ttcgccgatc
      961 tgctcgagcc ggccatcgaa ctagccgagc gcggccatgc ggtgtctgtc gtggtgcagc
     1021 agaaatgggc cgtcgcggct gaggagctcg cagcccagcc gggctttcgc gaggtcttcc
     1081 tgccctgggg ccgcgcgccg caggtgggcg agctgttccg caatccgggc gccgcgaagg
     1141 cgctgcgcct catcgcggaa accaaggggg cggcctacta ccagggcgag atcgccgccg
     1201 ccgcggccac ccacgcccgc cagcatggcg gggcgatcac ggccgaggac ttcgccgcct
     1261 accaagccga atgggtggcg cccatcgcac aggactaccg cggctacacg ctgcacgaga
     1321 tcccgcccaa cggccagggc attgccgcgc tgatcgcttt gggcatcctg tcccgcttcg
     1381 acctggccgc cttgccgccc gacagcgtcg aggcccagca tctgcagatc gaggcgatga
     1441 agctggcctt tgcggacgtg taccgctacg tcgccgagcc gcgcagcatg gagctcacgc
     1501 cggcgcagat gctcgatgcc ggctatctcg cggcgcgcgc gagctcgatc gacatgaagc
     1561 gcgctcagga cttcggcgcc ggcaaccctg ccaagggcgg caccgtatat ctgaccacgg
     1621 ccgacgagag cggcatgatg gtgagcttca tccagagtaa ctacatgggc tttggctccg
     1681 gcgtcgtggt gccacagttc ggcctgtcga tgcagaaccg cggtcatgcc ttctcgctgg
     1741 ccgcggctgg ccccaatgtc gtggcgccgg gcaagcggcc cttccacacc atcatcccgg
     1801 ccttcctcac tcgtggcggc gagccggtca tgtcctacgg cgtgatgggc gccaacatgc
     1861 agccgcaggg gcatgtgcag accctggtgc gcatgctcga ctaccggcag aacccgcagg
     1921 ctgcctgcga cgcgccgcgc tggcgctaca acgcagggct ggaactcaat gtcgaggcat
     1981 cgatggaccc ggcagtggtg caagg
//
LOCUS       DASUCY010000595         5177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_110303, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000595 DASUCY010000000
VERSION     DASUCY010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 5177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5177
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110303"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(236..311)
                     /locus_tag="VFV25_11725"
     tRNA            complement(236..311)
                     /locus_tag="VFV25_11725"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(276..278),aa:Lys,seq:ctt)
     gene            complement(320..395)
                     /locus_tag="VFV25_11730"
     tRNA            complement(320..395)
                     /locus_tag="VFV25_11730"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(360..362),aa:Lys,seq:ttt)
     gene            complement(447..1259)
                     /locus_tag="VFV25_11735"
     CDS             complement(447..1259)
                     /locus_tag="VFV25_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HEY1394038.1"
                     /translation="MQPLAPAAIPAQTVPDDAQDTERRFGGLRRLYGDAGYARLRAAR
                     IAVVGLGGVGSWAAEALARSGVAALTLIDLDHIAESNVNRQVHALDATLGQAKALALK
                     ERIAQIHAQCEVDAVEEFVDETNVETLLDGDRLDGVIDCCDQVRAKAALAAWSLHCGV
                     PLVTVGAAGGKRLAQAVELADLAEASHDPLLASLRQRLRQRHGAARKGAMGLRCVFSR
                     EPVQMPAGDANCEVDGSLNCHGYGSVVTVTAAFGLAAAGEMLNALAARERNL"
     gene            complement(1305..2039)
                     /gene="ybgF"
                     /locus_tag="VFV25_11740"
     CDS             complement(1305..2039)
                     /gene="ybgF"
                     /locus_tag="VFV25_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEY1394039.1"
                     /translation="MLALGACLLLLPAAHAGLFDDDEARKAILDLRQKQDQAAARQAE
                     TGDQIKQLRGSLLDLNTQLEQLRGEIAKLRGQNEQLARDLSEVQRRQQDLKQGFDDRV
                     RKLEPQKVTVDGKEFLTDPEEKRQYDEAFATLRKGDFAAGAAALSTFKARYPASGYNE
                     SVTFWLGNAQYGQKAYKEAIASFRALVTMAPDNPHASEALLSIANCQLELKDRVSARR
                     TLNELLKNYPKSEAAQAGRERLAALK"
     gene            complement(2104..2634)
                     /gene="pal"
                     /locus_tag="VFV25_11745"
     CDS             complement(2104..2634)
                     /gene="pal"
                     /locus_tag="VFV25_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEY1394040.1"
                     /translation="MMTAFRSFAPLALAILALAGCASGVNLDEPKVESRTPTLTSNDA
                     KASQGGQSQVANVDLGKQGSSAVGLQRIVYFDFDSYVVKEDYRPVIEGNAKLLNTDRK
                     KRMTIEGHTDERGGSEYNLALGQKRAEAVLKSLTLLGAQDAQLEAVSYGKERPAVSGS
                     DEAAWAKNRRAELRDR"
     gene            complement(2714..3937)
                     /gene="tolB"
                     /locus_tag="VFV25_11750"
     CDS             complement(2714..3937)
                     /gene="tolB"
                     /locus_tag="VFV25_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HEY1394041.1"
                     /translation="MAPARAQFRVEISGVGATQVPIAVARFRDEDKAPQQISAIIRAD
                     LERSGLFRNYDTSAIVLDELARPTMADWRAKAVDALVGGSVTRLADGRYDVRYRLWDV
                     VKGVDQGGQSLAVPVQDLRLAAHRIADQVYEKLTGEKGVFSTRIAYVSKTGGRSTLWV
                     ADADGEAAQSALGSPEPIISPDWSPDGKELAYVSFESRKPVVYVQEVASGRRKAVANF
                     RGSNSAPAWSPDGQRLVVTLTRDGISQLYVMNRDGSNLRRLTNSGAIDTEAVWSHDGR
                     SIYFVSDRGGGPQIYRTGVDGGSAERVTFSGAYNISPDISPDGRNLAYVTRQGNAFQL
                     QLMDLGSGTVAPLTDTTYDESPSFAPNGRLIIYATRVQGREVLMTTTLDGKIKARLQS
                     AQSDVREPTWGPYGR"
     gene            complement(4011..4811)
                     /locus_tag="VFV25_11755"
     CDS             complement(4011..4811)
                     /locus_tag="VFV25_11755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEY1394042.1"
                     /translation="MSLSVIVITRNEAANIEACLASASFADEWVVVDSNSSDGTREIA
                     ERFGARVIETHDWPGFGPQKNRALDEARCDWVLSLDADERVTPELAQQIQAAMRASEG
                     GGTAGYTLSRLSSFCGQWMRHGDWYPDHVLRLFRRGSARFSNDLVHERLECRGTIAHL
                     SAHLLHDSMPSLDACLDKMNRYSTGRARDQHRVGKKGGLGRAIAHGLWAFLRSYLLRG
                     GLLDGRMGFVVAFSIAEGTYYRYLKLWMLARDASSSPTIAIGKGIDAG"
     gene            4942..>5177
                     /locus_tag="VFV25_11760"
     CDS             4942..>5177
                     /locus_tag="VFV25_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="involved in the transport of lipid A across the
                     inner membrane; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid ABC transporter permease/ATP-binding
                     protein"
                     /protein_id="HEY1394043.1"
                     /translation="MSFFKNPTLARLRALQPYFKGTGWAVALTLVGAAVGASTESTMA
                     LLMRPLMDQGFQHRGLSLWQIPLAIIGLFFVRGIA"
BASE COUNT          833 a         1736 c         1687 g          921 t
ORIGIN      
        1 gaagcaagac ttccagttcc gcgacctgcg cgcaaaggct gcgaccgaga cggacgaggc
       61 cgctggcacg cgcagtgcgc aagccctgct gggccacacc accgagacga tgacggccac
      121 ctacatccgc cacaaagcgg gcaagaaagt gaggccgctc aagtgatttt gcggaacatg
      181 ttccgcaaaa acgaaggccg gttagcgggc actttcgcta accggccttg tattctggtg
      241 ggccctgagt gactcgaaca ctcgacctac ggattaagag tccgctgctc taccaactga
      301 gctaagagcc ccacttgtct ggtgggttgt gcaggattcg aacctgcgac caacggatta
      361 aaagtccgct gctctaccga ctgagctaac aacccgcgaa gcccaatatt atagctaggc
      421 ctgtacgaat tgcgcaaacg ggtattctac agattgcgct ccctggccgc aagcgcgttg
      481 agcatctcgc ccgccgcggc gaggccgaag gccgccgtca cggtcaccac cgaaccatag
      541 ccgtggcagt tgaggctgcc gtcgacttcg cagttggcgt caccggcagg catttgcacc
      601 ggctcgcgcg agaacacgca gcgcaggccc atcgcgccct tgcgcgcggc gccatggcgc
      661 tggcgcaggc gttgtcgcag cgaggcgagc aaggggtcat ggctggcctc ggccagatcg
      721 gccagctcga ccgcctgggc gaggcgcttg ccgccggcag cacccaccgt cacgagcgga
      781 acgccgcagt gcaggctcca tgccgcaagg gctgccttgg cgcgcacctg gtcgcagcaa
      841 tcgatcacgc cgtcgagcct gtcgccatcg agcagggttt cgacattggt ttcgtcgacg
      901 aactcctcga ccgcatcgac ctcgcattgc gcatggatct gcgcgatgcg ctccttgagc
      961 gccagcgcct tggcttgccc cagcgtggcg tccagcgcgt gcacttggcg gttgacgttg
     1021 gactcggcga tatggtccag atcgatcagc gtcagcgcag ccacgccgct gcgcgccagc
     1081 gcctcggccg cccaggagcc gacgccgccc agccccacca cggcaatgcg tgccgcgcgc
     1141 agccgggcat acccggcgtc cccatagagg cgccgcaagc cgccgaagcg gcgctcggta
     1201 tcctgcgcgt cgtcgggaac agtctgggcc ggtatcgcgg ccggggctag cggttgcagt
     1261 ttgtgcacgc ctgcattcta ggcaggcgtg caccggcgcc tttgttactt cagcgcggcc
     1321 agtcgctcgc ggccggcctg agccgcctcg gacttggggt agttcttcag cagctcgttc
     1381 aaggtacggc gcgccgagac gcgatccttg agttcgagct ggcagttggc gatcgacagc
     1441 agggcctcgg aggcatgcgg gttgtcgggc gccatcgtca ccagggcgcg gaaggaagcg
     1501 atagcctcct tgtaggcttt ctggccgtat tgcgcattgc ccagccagaa ggtcacggac
     1561 tcgttgtagc cgctggccgg atagcgtgcc ttgaaggtcg acagcgccgc cgcgccggcg
     1621 gcgaaatcgc ccttgcgcaa ggttgcaaag gcttcgtcgt attggcgctt ttcttccgga
     1681 tcggtgagga attccttgcc gtcgaccgtc actttctgcg gctccagctt gcgcacacgg
     1741 tcgtcgaagc cctgcttcag gtcctgctgg cggcgctgca cctcggacag atcgcgcgcc
     1801 agctgctcgt tctggccgcg cagcttggcg atttcgccgc gcagctgctc gagctgggtg
     1861 ttgaggtcca gcaggctgcc gcgcagctgc ttgatctggt cgcccgtctc ggcctggcgc
     1921 gcagcggcct ggtcctgctt ctggcgcagg tcgaggatcg ccttgcgcgc ctcgtcgtcg
     1981 tcgaacaggc ctgcgtgtgc ggccggcagc agtagcaggc aggcgcccag cgcaagcatc
     2041 gcggcgcggg cgcgctcggc agtcatcatc gtcttcatcg ttcgatcaac ctcactcacg
     2101 gtttcagcga tctcttagtt cggcgcggcg attcttggcc caggccgctt cgtcgctccc
     2161 gctcaccgcg gggcgctcct tgccgtagct cacggcttcg agctgggcgt cctgcgcacc
     2221 cagcagcgtg agcgatttca gcactgcctc ggcgcgcttc tgtcccagcg caaggttgta
     2281 ttcgctgccg ccgcgctcgt cggtgtgacc ttcgatggtc atgcgcttct tgcggtcggt
     2341 gttcaggagc ttggcattgc cctcaatcac cgggcgatag tcttccttca ccacatagct
     2401 gtcgaaatcg aagtagacga tgcgctgcag gcccaccgcg ctgctgccct gcttgccgag
     2461 gtccacgttg gcaacctggc tctggccgcc ctgcgatgcc ttggcatcat tggaggtcaa
     2521 ggtcggcgtg cggctttcca ccttgggttc gtcaaggttg acacccgagg cgcagcccgc
     2581 cagggcgagg attgcgagcg ccagcggcgc aaagctgcga aatgccgtca tcatggttca
     2641 actccaatct tgttcatcga gcttgttgat tgagcaggcc ggagccgctt gagggtggtc
     2701 gttccggaaa ggttcagcgg ccgtaagggc cccaggtcgg ctcgcgcaca tcgctctggg
     2761 cggactgcaa gcgcgccttg atcttgccat ccagcgtagt ggtcatcaat acttcgcgtc
     2821 cctggacccg ggtcgcgtag atgatgaggc ggccgttggg cgcgaagctc gggctctcgt
     2881 cataagtggt gtcagtcagc ggcgcgacgg tgccgctgcc gagatccatc agctggagct
     2941 ggaaggcatt gccctggcgc gtgacatagg ccaggttgcg cccatcgggg ctgatatcgg
     3001 ggctgatgtt gtaggcgccc gagaaggtga cgcgctccgc actgccgccg tcgacgcctg
     3061 tgcgatagat ttgcgggccg ccgccgcggt cgctgacgaa atagatcgag cggccgtcgt
     3121 gcgaccacac cgcttcggtg tcgatcgctc cgctgttggt gagccggcgc agattgctgc
     3181 cgtcgcggtt catcacatag agctgcgaaa tgccgtcgcg cgtcagcgtc acgacaagcc
     3241 gctggccgtc cggcgaccag gctggcgcgc tattgctgcc gcggaaattg gccaccgcct
     3301 tgcggcgtcc gctggccact tcctgcacgt agacaacagg tttgcggctc tcgaaggaga
     3361 cataggccag ctccttgcca tcgggcgacc agtccggcga aatgatgggc tcggggctgc
     3421 ccagcgccga ctgtgcggcc tcgccgtctg catcggccac ccacagcgtg gagcgcccgc
     3481 ctgtcttgct cacataggcg atgcgcgtgg agaacacgcc cttctcgccg gtgagcttct
     3541 catagacctg atcggcgatg cggtgcgccg caaggcgcag gtcttgcacc ggcaccgcga
     3601 ggctctgacc gccctggtcc acccccttga ccacgtccca cagccggtag cgcacgtcat
     3661 agcggccatc ggccaggcgt gtcaccgagc cgccgaccag cgcatcgacg gccttggcgc
     3721 gccagtcggc cattgtcggc cgagccagct cgtcgaggac gatggcgctg gtgtcgtagt
     3781 tgcgaaacag gccgctgcgc tccaggtcgg cgcgaatgat ggccgagatc tgctgcgggg
     3841 ccttgtcctc gtcgcggaag cgggccacgg cgatcggcac ctgcgtcgca ccgacgcctg
     3901 agatctccac gcggaactgc gcccgcgccg gtgccatgcc cgcgaacccg agggacaggc
     3961 ccaggggcac acccaaaaac gagcgccggc tccacgccgg ctgcaagcgg tcatcctgca
     4021 tcgataccct ttcctattgc gatggtcggc gatgatgacg catcgcgcgc cagcatccac
     4081 aacttgagat aacggtaata agtgccttcg gcgatgctga aggcgacgac gaagcccatg
     4141 cggccgtcga gcagcccgcc gcgcagcaga taagagcgca ggaaggccca caggccgtgc
     4201 gcgatcgcgc gccccaggcc ccccttcttg cccacgcggt gctgatcgcg cgcccgcccg
     4261 gtcgagtagc ggttcatctt gtccaggcag gcgtccaggc tgggcatgct gtcatgcaga
     4321 agatgagcgc tcagatgcgc aatggttcca cggcactcca ggcgctcgtg taccagatcg
     4381 ttgctgaagc gggcgctgcc gcggcggaag aggcgcagca cgtgatcagg ataccaatcg
     4441 ccgtggcgca tccattgccc gcagaaggag gacaggcgcg acagcgtata gccggctgtg
     4501 ccgccgcctt ccgatgctcg catcgcggcc tggatctgct gtgcgagctc gggcgtgacc
     4561 cgctcgtcag catccaggct cagcacccag tcgcagcgag cctcgtccag cgcgcggttc
     4621 ttctgcgggc cgaagccggg ccagtcatgg gtctcgatca cgcgggcgcc gaagcgctcg
     4681 gcgatctcgc gcgtaccgtc gctgctgttc gagtcgacca ccacccactc gtccgcgaag
     4741 gacgcagagg caaggcaggc ctcgatattg gccgcctcgt tgcgggtgat gacgatgacc
     4801 gatagagaca tgagcagtgc atagcgcctg agcaggcgac gcaggattgt aggcagcccg
     4861 gcgccgaagc cgtccgccgc ggcactgccg cgcataatcc ggcttcgcgc ggccccaggc
     4921 cgcgcccctg ttcgcgctcg catgtccttc ttcaagaacc cgaccctggc ccgcctacgc
     4981 gccctgcagc cctacttcaa gggcacgggc tgggccgtcg ccctgaccct ggttggagcc
     5041 gccgtaggcg ccagcaccga gtcgacgatg gctctgctga tgaggccgct gatggaccaa
     5101 ggcttccagc accgggggct gtcgctatgg cagatcccgc tggccatcat cggcctcttc
     5161 ttcgtgcgcg gcatcgc
//
LOCUS       DASUCY010000596         2426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_624210, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000596 DASUCY010000000
VERSION     DASUCY010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624210"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..>2426)
                     /gene="xdhB"
                     /locus_tag="VFV25_11765"
     CDS             complement(169..>2426)
                     /gene="xdhB"
                     /locus_tag="VFV25_11765"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdopterin binding
                     subunit"
                     /protein_id="HEY1394044.1"
                     /translation="HVAGAAAYIDDLPELAGTLHAALGLSPVAHGRIKAIDADKIRAL
                     PGVVAVLTAADIPGVNDCGPVIHDDPILAESEVHFLGQPVFAVIATDRELARRAAAQA
                     KQVLDIEPLPALLTPQAAHAAQSYVLPPMHMIRGDAKTAIAKAPHRLKGKLSVGGQEQ
                     FYLEGQISYAIPTENDGLHVHCSTQHPSEMQHAVAHVLGLHAHHVHVECRRMGGGFGG
                     KESQSAIFACVASIAAKKLGRPVKLRVDRDDDFMITGRRHGFEYEYEVGYDDEGRMLG
                     AEIEMVSNAGFSADLSGPVMTRALCHFDNAYWLPDVAIHGYSARTNTQSNTAFRGFGG
                     PQGAIASEVILDSIARALGKDALEVRRANFYGAGERNVTPYGQRVEDNVIRELVDELT
                     TKADYAARRTQIAAFNAASPVLKKGLALTPVKFGISFNVAHFNQAGALVHVYSDGSML
                     VNHGGTEMGQGLNTKVAQVVAHELGVSFETVRVTATDTQKVANTSATAASTGSDLNGK
                     AAQDAARQIRERLTAFAALRHGGQAKDVRFANDVVTVNGKQIPFPEFARSAYEARVQL
                     WSDGFYATPGLHWDRDKLQGRPFYYFAYGAALSEVVVDTLTGEWKLLRADVLHDVGRS
                     LNPAVDIGQIEGAFIQGMGWLTMEELVWHPQTGLLMTHAPSTYKIPTANDCPPVFNVR
                     LYENDNVEDSIHRSKAVGEPPLLLPFSVFLAIRDAVSSVGDHKVDPPLRAPATCEAIL
                     DAIEAVARGGA"
BASE COUNT          386 a          791 c          828 g          421 t
ORIGIN      
        1 gcgagcaccg cagagcagtc cgcgcgtcca gcgcggaccg agcacggagg gtcgcctctc
       61 ttttggttcc ttttctttgg cgaagcaaag aaaagggact cgcccgccgg ggcgaactcc
      121 cggcgcagcc tcacgcagtg agatcaagca ggccacaaca cacaaccctc aggcgccgcc
      181 tcgcgctacc gcctcaatgg catccaatat cgcctcacaa gtcgccggcg cccgcaacgg
      241 cggatccacc ttgtgatctc ctacagaaga aaccgcatcg cgtatcgcga gaaacaccga
      301 gaacggcagc agcagcggcg gctcgccgac ggccttggag cggtggatgc tgtcctcgac
      361 gttgtcgttc tcgtacagcc tcacgttgaa caccggcggg cagtcgttgg cggtggggat
      421 cttgtaggtg ctgggcgcgt gcgtcatcag caggccggtc tgcgggtgcc acaccagctc
      481 ttccatcgtc agccagccca tgccctggat gaaggcgcct tcgatctggc cgatgtcgac
      541 ggccgggttc agcgagcgac cgacgtcgtg cagcacgtcg gcgcgcagca gcttccattc
      601 gccggtcagc gtgtccacca ccacctcgct cagggccgcg ccataggcga agtaatagaa
      661 gggccggccc tgcagcttgt cgcggtccca gtgcaggcct ggggtcgcat aaaagccgtc
      721 cgaccacagc tgcacgcggg cctcataggc gctgcgcgcg aattcgggga aggggatctg
      781 cttgccgttc accgtcacca cgtcgttggc gaagcgcaca tccttcgcct ggccgccgtg
      841 gcgcagtgcg gcgaaggccg tcagccgctc gcggatctgg cgtgccgcgt cctgcgccgc
      901 cttgccgttc agatcgctgc cggtggaggc cgcagtggcc gaggtgttgg cgaccttttg
      961 ggtgtcggtg gccgtgacgc gcacggtctc gaagctcacg cccagttcgt gggcgaccac
     1021 ctgcgcgacc ttggtgttga ggccctggcc catctcggtg ccgccgtggt tcacgagcat
     1081 ggagccgtcg ctgtagacat gcaccagcgc gccggcctgg ttgaagtgcg ccacgttgaa
     1141 ggagatgccg aacttcaccg gcgtcagcgc cagtcccttc ttcagcaccg ggctggcggc
     1201 attgaaggcc gcgatctggg tgcgccgcgc cgcatagtcg gccttggtgg tgagctcgtc
     1261 caccagctcg cggatcacgt tgtcttcgac gcgctggcca tagggcgtga cattgcgctc
     1321 gcccgcgcca tagaagttcg cgcgacgtac ttccagcgca tccttgccca gcgcacgcgc
     1381 gatggaatcg aggatgactt cggacgcgat cgcgccctgc gggccaccga agccgcggaa
     1441 ggcggtgttg ctctgggtgt tggtgcgcgc gctgtagccg tggatggcca catcgggcag
     1501 ccaataggca ttgtcgaaat ggcacagcgc ccgcgtcatc accgggccgg acagatcggc
     1561 cgagaagccg gcgttggaca ccatctcgat ctcggcgccg agcatgcggc cttcatcgtc
     1621 atagccgacc tcgtactcgt actcgaagcc gtgccgccgg ccggtgatca tgaaatcgtc
     1681 gtcgcggtcc acgcgcagtt tgacggggcg gccgagcttc ttcgcggcga tggaggcgac
     1741 gcaggcgaag atcgccgatt gcgactcctt gccgccgaag ccgccgccca tgcgccggca
     1801 ttccacgtgc acgtggtggg catgcaggcc caggacatgg gccaccgcat gctgcatctc
     1861 gctcgggtgc tgggtagagc aatgcacgtg caggccgtcg ttctcggtcg ggatcgcgta
     1921 ggagatctgg ccttccaggt agaactgctc ctggccgccg acgctgagct tgcccttgag
     1981 ccggtgcggc gccttggcga tggcagtttt tgcgtcccca cgaatcatgt gcatgggcgg
     2041 cagcacatag ctttgcgcgg cgtgggctgc ctggggcgtc agcagggctg gcaggggctc
     2101 aatgtccagc acctgtttgg cttgcgcggc ggcgcgccgc gcaagctcgc gatcggtggc
     2161 gatcactgcg aacaccggct ggccgaggaa atggacctcg ctctcggcga ggatgggatc
     2221 gtcgtgaatg acgggtccgc agtcgttgac gccggggatg tcggccgccg tcaacacggc
     2281 gaccacgcca ggcagggcgc ggatcttgtc ggcatcgatg gccttgatgc gaccatgggc
     2341 cacgggcgag aggcccagcg ccgcgtgcag cgtgccggcg agttcgggca gatcgtcgat
     2401 ataggcggct gcgccggcca cgtgca
//
LOCUS       DASUCY010000597         2154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1106084, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000597 DASUCY010000000
VERSION     DASUCY010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106084"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VFV25_11770"
     CDS             complement(<1..551)
                     /locus_tag="VFV25_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1394045.1"
                     /translation="MAKQLVAVTPPVRQGALAKVAIDFSEFKLGWRILVVALLGVGVN
                     ANASMLYAFGAMVIPLQKALGWTRPELQTAIAFLFGGAVIGSQLVGWLNRRYGMRVVT
                     MVSLIALSVVFLAHTLIRGSIAWLYLCFFILPIASMGTMQVTWTQIVNLWFERNRGLG
                     LAIVLSGTGLAAALVPIGVTWAVG"
     gene            complement(558..1748)
                     /locus_tag="VFV25_11775"
     CDS             complement(558..1748)
                     /locus_tag="VFV25_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005666934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEY1394046.1"
                     /translation="MAERLFEKNVAITGIGQSEVGRPSSRSPMQLTLDAALEAIADAG
                     LKREDIDGIACWPGDNQNGSPFSPVGPNALMSALGLQVNWYGGGYEGPGPLTGVINGA
                     MAIAAGLCRHVLVFRTITEASGRLLDKSASALTHKTSGRDSSYFWQWYTPFNVLSAIN
                     LVAMYAQRHFHEYGTTPEQLAQIALTCRRHAAANPKAVFREPMTLDDYFASKMISTPL
                     RMFDCDVHCDASTALVLSRADAAKDGPNVPIRIEAVGSALHQPWSWDQISLTGMAAHD
                     VGRMMWSRTDLKPKDVGSAQLYDGFSILTMIWLEGLGLCPQGESGRFVEGGGNIARDG
                     VLPLNTNGGQLSAGRTHGLGYVHEACTQLWGRGGDRQIAPHRVAVTAAGGGPLGGSLL
                     LVKE"
     gene            complement(1748..>2154)
                     /locus_tag="VFV25_11780"
     CDS             complement(1748..>2154)
                     /locus_tag="VFV25_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEY1394047.1"
                     /translation="RKLPALTPDTAPFWQGGANGTLLMHHCGGCNRWFHPPAPACPRC
                     GSFDVAPRAVSGRGTVATFTVNHQPWTKDLRLPYVVAIVELAEQAGLRLVTNVVGCAP
                     EDVCIDMPVRVRFEPHEEVWLPLFEPDPREGA"
BASE COUNT          368 a          725 c          737 g          324 t
ORIGIN      
        1 ccgaccgccc aggtgacgcc gatcgggacc agcgcggccg cgaggccggt gccagaaagc
       61 acgatcgcca ggcccaggcc gcgattgcgt tcgaaccaga ggttgacgat ctgcgtccag
      121 gtcacttgca tcgtgcccat actcgcgatg ggcaggatga aaaagcagag gtagagccag
      181 gcgatgctgc cacggatcag ggtgtgcgcc aagaacacga cggacagcgc aatcaacgac
      241 accatggtga cgacgcgcat gccatagcgc cgattgagcc agcccacgag ttgcgagccg
      301 atcactgcgc cgccgaagag gaacgcgatg gccgtctgca gttccgggcg cgtccagccc
      361 aacgccttct gcagcgggat gaccatagcg ccgaaggcgt agagcatcga cgcgttggcg
      421 ttgacgccga cacccagcag cgccaccacg agaatgcgcc agccgagctt gaactcgctg
      481 aagtcgatgg cgaccttggc aagcgcgcct tgccggaccg gcggggtgac cgcgacgagt
      541 tgcttggcca tggtcagtta ttccttcacc agcagaagac ttccgccgag cggcccaccc
      601 ccggcggccg tcaccgcgac acggtgcggc gcgatctgcc gatccccgcc acgaccccag
      661 agctgggtgc aggcctcgtg gacgtagccc aggccgtgcg tgcggccggc ggacagctgg
      721 ccgccattgg tgttgagcgg cagcacgcca tcgcgagcga tgtttccgcc gccttcgacg
      781 aagcggccgc tctcgccttg cgggcacagg ccgaggcctt cgagccagat catcgtcagg
      841 atggaaaagc cgtcgtacag ctgcgcgctg ccgacgtctt tgggcttgag gtccgtgcgc
      901 gaccacatca tgcggcccac gtcgtgcgcc gccatgccgg tcagcgagat ctggtcccac
      961 gaccacggct ggtgcagcgc cgagccaacg gcctcgatgc ggatcggcac gttcgggccg
     1021 tccttcgcgg cgtcggcccg cgacaggaca agcgcggtgg aggcatcgca gtgcacgtcg
     1081 cagtcgaaca tgcgcagcgg cgtagagatc atcttcgacg cgaagtagtc atccagcgtc
     1141 atcggctctc ggaacacggc cttcgggttc gcggcggcat ggcgccggca ggtgagcgca
     1201 atctgcgcga gctgctcggg cgtcgtgccg tactcgtgga aatggcgctg ggcatacatc
     1261 gcgaccaggt tgatcgctga caggacgttg aagggcgtgt accactgcca gaagtagctg
     1321 ctgtcgcgcc cgctggtctt gtgggtgagg gcgcttgccg acttgtccag cagccgcccg
     1381 gaagcctcgg tgatggtgcg aaacaccagc acgtgccggc acagcccggc agcgatcgcc
     1441 atcgcaccgt tgatgacacc ggtgagcgga ccgggccctt cgtagccgcc gccgtaccag
     1501 ttcacctgta ggcctagcgc gctcatgagc gcgttgggtc cgaccggaga aaaggggctg
     1561 ccgttctggt tgtcgcccgg ccagcaggcg atgccgtcga tgtcctcgcg cttcagcccc
     1621 gcatcggcga tcgcttcgag cgccgcatcg agcgtcaatt gcattggcga gcgcgacgac
     1681 gggcgcccga cctccgactg gccgatgccc gtgatggcga cgttcttctc gaacaggcgc
     1741 tcggccatca cgcaccttcc cgcgggtcgg gctcgaacag gggcagccag acttcctcgt
     1801 gcggctcgaa gcgtacccgc accggcatgt cgatgcacac gtcctcgggc gcgcaaccca
     1861 cgacgttggt gaccaaccgc aggcccgcct gctcggcgag ctcgacgatg gcgacgacat
     1921 agggcagccg caggtccttc gtccacggct ggtggttgac ggtgaaggtg gcgacggtgc
     1981 cccggcccga caccgcccgc ggcgccacgt cgaagctgcc gcagcgcggg catgccggcg
     2041 ccggcgggtg gaaccagcgg ttgcagccac cgcagtgatg catgagcagc gtgccgttcg
     2101 cgccgccctg ccagaacggc gctgtgtcgg gcgtgagggc cggaagcttg cgcg
//
LOCUS       DASUCY010000598         2670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1507891, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000598 DASUCY010000000
VERSION     DASUCY010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1507891"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VFV25_11785"
     CDS             complement(<1..655)
                     /locus_tag="VFV25_11785"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEY1394048.1"
                     /translation="MALRINDLAPDFTAQTTQGSINFHQWIGDSWAILFSHPKDFTPV
                     CTTELGYMAKIEPEFAKRNCKLIGLSVDPVASHQSWSKDIEETQGYLPKYPMIGDPDL
                     AVSKLYNMLPAEEPGTSEGRTPAANATVRSVFMIGPDKRIKLMLTYPMTTGRNFDEIL
                     RVLDSMQLTAKHRVATPVNWKQGEDVIIAGSVSDDEAKTLFPQGWKAPKPYLRIVKQP
                     "
     gene            838..1431
                     /locus_tag="VFV25_11790"
     CDS             838..1431
                     /locus_tag="VFV25_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEY1394049.1"
                     /translation="MTVTQIVTLAVLAVLAFWAVGAYNRLVQLRNAIGNAFAQIDVQL
                     KRRYDLIPNLVETAKRYLQHEHETLEAVTAARSQALAAADAVRSRPGAAGPVKSLAMA
                     EQVLGGAMGRLMALVEAYPELKADQTQRELSEELTSTENKVAFSRQLFNDAAMDYNVA
                     AQQFPTSLLAGVFGFRQAAMLQATTSDAERAAVKVQF"
     gene            1481..>2670
                     /locus_tag="VFV25_11795"
     CDS             1481..>2670
                     /locus_tag="VFV25_11795"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEY1394050.1"
                     /translation="MRFRRQQDEARAATLRLLLLFALLLLAVVVAVNLALAGLYSLLS
                     LGGLAYPPLFFETNTAVVLLFVLGGCAVEAGRLRDAGGARVALAVGGREIVEARDLAE
                     RRLLNVLDEMALASGLPRPTPFVLEHEPGINAFVAGWGHGDDGRERRGPIVLGVTRGA
                     LECLTRDELQGLVAHELGHIKAGDLRLQMWLLALVWGLSLVHGYGRRLGEPDDQGRPN
                     LPGLLFGAVFVVLGWLGWLAGRVLQAAVSRQREFGADAYAVQFTRSRDGLGGTLRKIR
                     FQSEIEHTGLQHPQAQLLAAMLLHAPARSRWLATHPPLAERIKRVCGRELPPLPAQPI
                     DLQAEAGQLGNTQASGQSTLDSRVAGGEDAPDHPWAESPAATPATPLLQAAAMTLDVN
                     SRPP"
BASE COUNT          403 a          869 c          952 g          446 t
ORIGIN      
        1 ctggctgctt gacgatgcgc aggtaaggct tgggcgcctt ccaaccctgc gggaagagcg
       61 tcttcgcctc gtcgtccgac accgagccgg cgatgatcac gtcttcgccc tgcttccagt
      121 tgactggcgt cgccacgcgg tgcttggccg tgagctgcat ggagtcgagc acgcgcagga
      181 tctcgtcgaa gttgcgaccg gtggtcatcg gataggtcag catcagcttg atgcgcttgt
      241 ccgggccgat catgaacacc gagcgcaccg tcgcattggc ggccggcgtg cggccttcgg
      301 aggtgccggg ctcctcggca ggcagcatgt tgtagagctt ggacaccgcg aggtcagggt
      361 cgccgatcat cgggtacttg ggcagatagc cctgggtctc ctcgatgtcc ttggaccagg
      421 actggtggct ggccaccgga tcgacgctca ggccgatcag cttgcagttg cgcttggcga
      481 actccggctc gatcttggcc atgtagccca gctcggtggt gcacaccggc gtgaagtcct
      541 tggggtgcga gaacagaatg gcccagctgt cgcctatcca ctggtggaag ttgatgctgc
      601 cttgcgtggt ctgggccgtg aaatcgggcg caaggtcatt gatgcgcaat gccatgatga
      661 ctccttcggt cagcaagcta gccaaacaac gcgggcggcg atcaccgtgg ggggcgaccg
      721 ccgtctgccg cgggggaaga acactgtgaa gtattgcccc agcggggcgc atttccaagg
      781 ggattggccg catgccgtgc agtagagtcc ccgccaacgc aaaggagaaa gccgccgatg
      841 accgtcaccc aaatcgtcac cctggccgtg ttggccgtgc tcgccttctg ggccgtgggc
      901 gcctacaacc gcctggtgca gctgcgcaat gcgataggca acgcctttgc gcagatcgac
      961 gtgcagctca agcgccgcta cgacttgatt cccaacctgg tcgagacggc caagcgctat
     1021 ctgcagcacg agcacgagac gctggaagcg gtgacggctg cgcgcagcca ggcgctggcg
     1081 gccgccgatg cggtgcgcag ccggcccggc gccgccggcc cggtgaagag cctggccatg
     1141 gccgagcagg tgctgggcgg agcgatgggg cggctgatgg ccctggtcga ggcctacccc
     1201 gagctcaagg ccgaccagac ccagcgcgag ctgtccgaag agctgaccag caccgagaac
     1261 aaggtggcct tttcgcgcca gctcttcaac gacgcggcga tggactacaa cgtcgcggcc
     1321 cagcagtttc cgacctcgct gctggccggg gtgttcggtt tccgccaggc agcgatgctg
     1381 caggcgacga ccagcgacgc cgaacgcgcc gcagtgaagg tgcagttcta gtgtcctgtc
     1441 cgcgtactgc gggagaggac gccaggcagg cgcacccggc atgaggtttc gccggcagca
     1501 ggacgaggcg cgcgcggcca cgctgcggct tctgctgctg ttcgccctgc tgctgctggc
     1561 ggtggtggtg gcggtcaatc tcgctttggc cgggctctac agcctactga gcttaggcgg
     1621 cctggcctat ccgccgctct tcttcgagac caataccgcc gtggtgctgc tcttcgtgct
     1681 gggcggctgc gccgtcgagg ccggccggct gcgcgatgcc ggcggcgcgc gcgtcgcgct
     1741 ggccgtgggc gggcgcgaga tcgtcgaggc gcgcgacctg gccgagcggc gcctcctcaa
     1801 cgtgctggac gagatggcgc tggcctcggg cctgccgcgc cccacgccat ttgtcctgga
     1861 gcatgagccc ggcatcaatg ccttcgtcgc gggctggggg cacggcgatg acggacggga
     1921 aaggcgcggt ccgatcgtgc tgggcgtcac gcgcggcgcg ctcgaatgcc tgacgcgcga
     1981 cgagctgcag gggctggtgg cgcacgagct cggccatatc aaggccggtg acctgcggct
     2041 gcagatgtgg ctgctggcgc tggtctgggg cctgtcgctg gtgcacggct acggacgcag
     2101 gctcggcgag cccgacgacc aagggcggcc caatctgcct ggcctgctgt tcggcgcggt
     2161 cttcgtcgtc ctcggctggc tgggctggct cgccggccgg gtgctgcagg cggccgtgtc
     2221 gcgccagcgc gaattcgggg ccgacgccta tgcggtgcaa ttcacgcgca gccgcgacgg
     2281 cctgggcggc acgctacgca agatccggtt ccagagcgag atcgagcata cggggctgca
     2341 gcatccgcag gcgcagctgc tggccgccat gctgctgcat gcgccggcgc gctcgcgctg
     2401 gctggccacc catccgccgc tcgcggagcg cattaagcgc gtctgcgggc gcgaactgcc
     2461 gccgctgccg gcgcagccaa tcgacttgca ggcagaggcc gggcagcttg gcaacacgca
     2521 agcaagtgga cagtccactc tggacagccg ggtcgcggga ggcgaggatg cgccggatca
     2581 tccctgggcc gaatcgcctg cggccactcc cgctacacct ttgctgcaag ccgctgccat
     2641 gactctggac gtgaattccc ggccgccgga
//
LOCUS       DASUCY010000599         7537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1523795, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000599 DASUCY010000000
VERSION     DASUCY010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1523795"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2486)
                     /locus_tag="VFV25_11800"
     CDS             complement(<1..2486)
                     /locus_tag="VFV25_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEY1394051.1"
                     /translation="MTDLSAEFKALAEYRPTHKVRFVTAASLFDGHDASINIMRRILM
                     SMGAEVIHLGHNRSVDEVVTAALQEDAQGIAVSSYQGGHVEYFKYMVELLKARGGENI
                     QVFGGGGGVIVPAEIRDLNEHGVRIFSPEDGQRMGLAGMIGEMLMRCDRDLGAQAPKK
                     LAAIEGQTESHWRALAQLITALENGQADAKLKAALHEAAKNGSGSKTPVLGITGTGGA
                     GKSSLTDELIRRLRLDQNDALHVAVISIDPSRRKSGGALLGDRIRMNAIQPWQQGPRV
                     YMRSLATREAGSEISAALPDVVAACRCAGFDLVIVETSGIGQGDAAIVPHVDVPMYVM
                     TPEYGAASQLEKIDMLDFAEFVAINKFDRKGAADALRDVSKQVQRNKEAFSKKPDQMP
                     VFGTVASRFNDDGVTALYQALLPRLAGLGLKLGESRLPQVHTRHSTHQTPIVPSARVR
                     YLAEISDTVRGYKARARQQAALAREVQQLRASTRMLTEAKQPTIPALEDLAAQRETEL
                     GTDAKKLLAQWPQMQQAYAGDEYVVKIRDKEIRTALVHTSLSGTKIRKVVLPHYEDHG
                     ELLKWLMLENVPGSYPYTAGVFAFKREGEDPTRMFAGEGDAFRTNRRFKMVSEGMPAK
                     RLSTAFDSVTLYGADPAPRPDIYGKVGNSGVSIATLDDMKVLYSGFDLTNPSTSVSMT
                     INGPAPTILAMFMNTAIDQNLEKFRADNGREPTDTEGEKIRAWVLENVRGTVQADILK
                     EDQGQNTCIFSTEFSLKVMGDIAEYFVHHNVRNFYSVSISGYHIAEAGANPISQLAFT
                     LSNGFTFVEAYLARGMHIDDFAPNLSFFFS"
     gene            2716..3258
                     /locus_tag="VFV25_11805"
     CDS             2716..3258
                     /locus_tag="VFV25_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1394052.1"
                     /translation="MPIIPTRSAEGQDELNRRARGLSQRHRTVLLLVDGRRSVEQVRQ
                     MAQQAGAPASCFDELVELGLVGLVQPAAAQAVDIALPAVAAEPPELPAADDVAAVTTR
                     DSALEAARELLVQALRAEAPVAGSITIMKVKRAASRREMADLLDDVQAKLSKARTGMA
                     HVTRRARDLLDGVDAAVSGT"
     gene            3331..4098
                     /locus_tag="VFV25_11810"
     CDS             3331..4098
                     /locus_tag="VFV25_11810"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEY1394053.1"
                     /translation="MSFLAIDVGNTRLKWAQYAAPHPGAELLSHGAVFLENIDQLSEG
                     EWAQLAPPSSMLGCCVAGDAVRHRVEEQLDMWELSPSWVVSSAAEGGVTNGYDHPARL
                     GTDRWVALIGAHGRMRAAGHAGPLLVAMVGTAVTVDALDAAGRFLGGFILPGHGIMLR
                     ALESGTAGLRVPTGEVTEFPTNTSDALTSGGTYAITGAIERMHRSIALRCGAAPRCII
                     TGGAGWKVAPYLAVPYELVESLIFDGLLYTAQRRNLK"
     gene            complement(4138..5481)
                     /gene="hslU"
                     /locus_tag="VFV25_11815"
     CDS             complement(4138..5481)
                     /gene="hslU"
                     /locus_tag="VFV25_11815"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEY1394054.1"
                     /translation="MSMTPQEIVSELDRHIVGQQDAKRAVAIAMRNRWRRQQVDEKLR
                     AEITPKNILMIGPTGVGKTEIARRLARLADAPFIKVEATKFTEVGYVGKDVDSIVRDL
                     VDMAVKQAREAAVRAHHTRAEDAAEERILDALVPPPRAPTLGFGSAAETAPAPDSTAR
                     QVMRKRLREGALDDKEIEIELAEAKPALEIMSPPGMEEMAEQLRGMFANLGSPRRKTR
                     KLKIAEAQKLLVDEEAAKLVNEDELKTQALHNAEQNGIVFIDEIDKVASRSEVGGADV
                     SRQGVQRDLLPLVEGTTINTKYGMVKTDHILFIASGAFHLSKPSDLIPELQGRFPIRV
                     ELGSLSVADFEAILTQTHASLVKQYQALLATEGVSLEFKPDAIRRLAQIAYDVNERTE
                     NIGARRLSTVLERLLEEVSFDAPKLEGRSVAIDAAYVDAKLDSLAKNEDLSRYIL"
     gene            complement(5545..6084)
                     /gene="hslV"
                     /locus_tag="VFV25_11820"
     CDS             complement(5545..6084)
                     /gene="hslV"
                     /locus_tag="VFV25_11820"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEY1394055.1"
                     /translation="METYHGTTIVSVRRGRQVALGGDGQVTLGNIVVKASARKVRKLY
                     RDQVLAGFAGATADAFTLFERFEAKLEKHQGHLVRAAIELTRDWRTDRVLRRLEAMLV
                     VADADASLIISGNGDVLEPEHGIVAIGSGGPYAQAAARALLDHTELSPQEIVKKSLEI
                     AGDLCIYTNQHHTIETLGD"
     gene            complement(6146..>7537)
                     /locus_tag="VFV25_11825"
     CDS             complement(6146..>7537)
                     /locus_tag="VFV25_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEY1394056.1"
                     /translation="KAKIDEIEQQMVGEGLNARGHAPARPSSFYNAPTQPAAIGDAPT
                     TPMHIGQRGEPRPAAPAAAAMPPAAVVMGAGMSPLSPFAAALGANDFANPFAVEVNEI
                     AHDPELDEAVIAFANADFELCEQSLSSLIGMGGSRAQHAETWLVLFDLYRAIGQQHKF
                     ESLALDYAQQFGWSAPQWFSLPLLVAEAAAAAEDKVKRSGARVDGSIGWVAPDMVDIE
                     AVAKLRAQTLQMPLPWVLDWNAVRSIDAEACHELSLLMRGWAGQNLDMRWLGGEHLLG
                     VLAECAPAGVRDADPAFWLLRLDALRLTNRADQFDEVAIDYCVTYEVSPPSWEPARCV
                     ARVSEGGRSTTAPVSVLTDVSTGFDETQQQDDGSMVPTASVELSGQLVGDIGQTLDRL
                     QGQLGAAAIVSVSCSRLIRVDFIAAGDLLNWVLARRGENRTVSFDDAHRLVALFFGAM
                     GINEHARVKVRNI"
BASE COUNT         1088 a         2519 c         2591 g         1339 t
ORIGIN      
        1 ttggagaaga agaacgacag gttgggcgcg aagtcgtcga tgtgcatgcc gcgcgcgaga
       61 tacgcctcga cgaacgtgaa gccgttggac agcgtgaagg cgagctgcga gatcgggttc
      121 gcgcccgcct cggcgatgtg gtagccgctg atcgacaccg agtagaagtt gcgcacgttg
      181 tggtgcacga agtactcggc gatgtcgccc atcaccttga gcgagaactc ggtcgagaag
      241 atgcaggtgt tctggccctg gtcttccttc aggatgtcgg cctgcaccgt gccgcgcacg
      301 ttctccagca cccaggcgcg gatcttctcg ccttccgtgt cggtcggctc gcggccgttg
      361 tcggcgcgga acttctccag gttctggtcg atcgcggtgt tcatgaacat cgcgaggatg
      421 gtgggcgccg ggccgttgat ggtcatcgac actgaggtgc tggggttggt caggtcgaag
      481 cccgaataga gcaccttcat gtcgtccagc gtcgcgatcg agacgccgga attgccgacc
      541 ttgccgtaga tgtcgggccg cggcgccggg tcggcgccat agagcgtcac cgagtcgaag
      601 gcggtggaca gccgcttggc aggcatgccc tcgctcacca tcttgaagcg gcggttggtg
      661 cggaaggcat cgccttcgcc ggcgaacatg cgcgtcgggt cctcgccctc gcgcttgaat
      721 gcaaaaacgc cggccgtgta ggggtagctg cccggcacgt tctccagcat cagccacttc
      781 agcagctcgc cgtggtcttc gtagtgcggc agcacgacct tgcggatctt ggtgcccgac
      841 agcgaggtgt ggaccagcgc ggtgcggatc tccttgtcgc ggatcttcac cacgtattca
      901 tcaccggcgt aggcctgctg catctgcggc cattgggcca gcagcttctt cgcgtcggtg
      961 ccgagctccg tttcgcgctg cgcggccaga tcctccagcg cggggatagt cggctgcttg
     1021 gcctcggtca gcatgcgtgt ggaggcgcgc agctgctgca cctcgcgtgc cagcgccgcc
     1081 tgctgccgag cacgtgcctt gtagccgcgc accgtgtcgc tgatctcggc caggtagcgc
     1141 acgcgcgcgg acggcacgat gggcgtctgg tgcgtgctgt ggcgggtgtg cacctggggc
     1201 agccggcttt cgcccagctt caggcccagc ccggccaggc gcggcagcag ggcctggtaa
     1261 agcgccgtca cgccgtcgtc gttgaagcgg ctggccacgg tgccgaacac cggcatctgg
     1321 tcgggcttct tggagaacgc ctccttgttg cgctgcactt gcttgctgac gtcgcgcagc
     1381 gcgtcggccg cgcccttgcg gtcgaacttg ttgatggcga cgaattcggc gaaatccagc
     1441 atgtcgatct tctcaagctg gctggcggcg ccgtattcgg gcgtcatcac atacatcggc
     1501 acgtccacat gcggcacgat ggctgcgtcg ccctggccga tgcccgaggt ctcgacgatc
     1561 acgaggtcga agccggcgca cctgcaggcg gcgaccacgt cgggcagggc ggcgctgatc
     1621 tcgctgccgg cctcgcgcgt ggcgaggctg cgcatataga ccctgggccc ttgctgccac
     1681 ggctggatgg cattcatgcg gatgcggtcg cccagcagcg cgccgccgct cttgcgccgc
     1741 gagggatcga tggagatcac cgcgacatgc agcgcatcgt tctggtccag gcgcaggcgg
     1801 cggatcagct cgtcggtgag cgagctcttg cccgcgccac cggtgccggt gatgcccagc
     1861 accggcgtct tcgagccact gccgttcttg gcggcctcgt gcagcgctgc cttgagcttg
     1921 gcatccgcct ggccgttttc cagcgcggtg atcaactggg ccagggcgcg ccagtggctt
     1981 tccgtctggc cctcgatggc ggcgagcttc ttgggcgcct gggcgccgag gtcgcggtcg
     2041 cagcgcatca gcatctcgcc gatcatgcct gccaagccca tgcgctggcc gtcctctggg
     2101 ctgaagatgc gcacgccgtg ctcgttcagg tcgcggatct cggccggcac gatcacgccg
     2161 ccgccgccgc cgaagacctg gatgttctcg ccgccgcgcg ccttcagcag ctcgaccatg
     2221 tacttgaagt attcgacgtg cccaccctgg taggagctca ccgcgatgcc ctgggcgtct
     2281 tcctgcaggg ccgctgtcac gacctcgtcg accgagcggt tgtggcccag gtggatcacc
     2341 tcggcgccca tgctcatcag gatgcgccgc atgatgttga tcgaggcgtc gtggccgtcg
     2401 aacaggctgg ccgcggtaac gaagcggacc ttgtgggtcg gccggtattc ggccagggct
     2461 ttgaattcgg cggacaggtc ggtcatgcat gtctccttgg ccggtgcata ccagctcggc
     2521 ctcgtttcga attgacgttt acgtaaacgt caatgatagt tcggcattat gacgctttgc
     2581 gctgccagct tcccgtcagc gtcgcggact gcgtactttg cacgccaagg ccgcctggcg
     2641 taaagccgga gggctttgcg ctggccgaac ggcctcacaa tgcggcccca gcctgaacga
     2701 gcgttcgctg ctgccatgcc tatcattccg acaagatccg ccgagggaca ggacgaactg
     2761 aaccgccgcg cgcgcggcct cagccagcgc catcgcacgg tgctgttgct ggtggacggg
     2821 cggcgcagcg tcgagcaggt gcggcagatg gcccagcagg ccggtgcgcc cgcctcctgt
     2881 ttcgatgagc tcgtggagct gggcctggtc ggcttggtgc agcctgccgc ggcgcaagcc
     2941 gtggacattg ccttgcccgc tgtcgcggcc gagccgcccg aactccctgc cgccgacgac
     3001 gtggccgccg tcaccactcg tgacagcgcg ctggaggccg cccgtgagct gctcgtgcag
     3061 gccctgcggg ccgaggcgcc ggtggcaggc tcgattacga tcatgaaggt caagcgcgcc
     3121 gccagccgcc gcgagatggc cgatctactc gacgatgtcc aggccaagct ttccaaggcg
     3181 cgaaccggca tggcgcatgt cacgcgccgg gcacgcgacc tgctggatgg ggtcgatgcc
     3241 gccgtctccg gaacctagcc acctcttgcg aagcgcggtg gggggttagc ccggctgcag
     3301 acccggcggc gttgacgcag actcgcgccc atgagctttc tcgccatcga cgtcggcaac
     3361 acccgcctca agtgggccca gtacgcggcg ccgcaccctg gtgccgagct gctgagccat
     3421 ggcgcggttt ttctcgagaa catcgaccag ctgagcgagg gcgaatgggc gcagctcgca
     3481 ccgccttcca gcatgctggg ctgctgcgtg gccggcgacg cggtccgcca ccgcgtcgag
     3541 gagcagctcg atatgtggga gctctcgccc agctgggtcg tctccagtgc cgccgaggga
     3601 ggcgtcacca acggctacga ccatcccgcc cggctcggca ccgaccgctg ggtcgcgctg
     3661 attggtgccc atgggcgcat gcgcgccgcc ggccatgccg gtccgctgct ggtggcgatg
     3721 gtgggtacgg ccgtcacggt ggacgccctg gacgccgccg gccgcttcct gggcggcttc
     3781 atcctgcccg gccacggcat catgctgcgc gcgctggaaa gcggcaccgc cgggttgcgc
     3841 gtgccgaccg gcgaggtgac cgaattcccc accaacacca gcgatgcgct gaccagcggc
     3901 ggcacctatg ccatcaccgg cgccatcgaa cgcatgcacc gcagcatagc tctgcgctgc
     3961 ggcgcggcgc cgcgctgcat catcacgggc ggcgcgggct ggaaggtggc gccctatctg
     4021 gccgtgccct acgagctggt cgagagcctg atcttcgatg ggctgctcta caccgcccag
     4081 cgtcggaatc tgaagtaaaa acccggcgtt accctgctta attcgccgcg cgctacatca
     4141 aagaatgtag cgcgacaaat cctcgttctt cgccaggctg tcgagcttgg catccacata
     4201 ggcggcgtcg atcgccacgc tccggccctc cagcttgggc gcgtcgaagc tcacttcctc
     4261 gagcaggcgc tccagcaccg tcgacagccg ccgcgcgccg atgttctcgg tgcgctcgtt
     4321 cacgtcgtaa gcgatctgcg ccaggcggcg gatcgcgtcg ggcttgaact ccaggctcac
     4381 gccctcggtc gccagcaggg cctggtactg cttgaccagg ctggcgtggg tctgggtcag
     4441 gatggcctcg aagtcagcca cgctgagcga gcccagctcg acgcggatcg ggaagcggcc
     4501 ctgcagctcg gggatcaggt cgctcggctt gctcaggtgg aaggcgcccg aggcgataaa
     4561 gaggatgtgg tcggtcttga ccatgccgta cttggtgttg atcgtcgtgc cctccaccag
     4621 cggcagcagg tcgcgctgca cgccctggcg cgagacatcg gcgccgccca cctccgagcg
     4681 cgaggccacc ttgtcgatct cgtcgatgaa gacgatgccg ttctgctcgg cgttgtgcag
     4741 ggcctgggtc ttgagctcgt cctcgttgac cagcttggcg gcctcctcgt cgaccagcag
     4801 cttctgcgcc tcggctatct tgagcttgcg cgtcttgcgc cgcggcgagc ccaggttggc
     4861 gaacatgccg cgcagctgct ctgccatctc ctccatgccg ggcgggctca tgatctccag
     4921 cgccggtttg gcttccgcga gctcgatctc gatttccttg tcgtcgagcg cgccttcgcg
     4981 caggcgcttg cgcatcacct ggcgcgcggt gctgtcgggc gccggtgccg tctcggcggc
     5041 cgagccgaag ccgagcgttg gcgcgcgcgg cggcggcacg agtgcgtcga ggatgcgctc
     5101 ctcggccgca tcctctgcgc gggtgtggtg ggcccgcacg gcggcctcgc gtgcctgctt
     5161 gacggccatg tcgaccaggt cgcgcacgat ggagtccacg tccttgccga catagccgac
     5221 ctcggtgaac ttggtcgcct cgaccttgat gaagggcgca tcggccagcc ttgccaggcg
     5281 gcgcgcgatc tcggtcttgc cgacgccggt ggggccgatc atcaggatgt tcttgggcgt
     5341 gatctcggcg cgcagcttct cgtccacctg ctggcgccgc cagcggttgc gcatcgcgat
     5401 cgccacggcg cgcttggcat cctgctggcc gacgatgtgg cggtcgagct cggagacgat
     5461 ctcctggggg gtcatgctca tgtcgtagtg acttgtgact ggtgactcgt gaccggtgag
     5521 tttcaagtca ccagtcacaa gtcactagtc acccaaagtc tcaatggtgt gatgctgatt
     5581 ggtgtagatg cacaggtcgc cggcgatctc cagcgacttc ttcacgatct cctgcgggct
     5641 cagctcggtg tggtcgagca gggcgcgcgc cgctgcctgg gcataggggc cgccggagcc
     5701 aattgcgacg atgccgtgct cgggctccag cacgtcgcca ttgccgctaa tgatgagcga
     5761 ggcatcggca tcggccacca ccagcatggc ttccaggcgg cgcagtacgc ggtcggtgcg
     5821 ccagtcgcgg gtcagctcga tcgccgcgcg caccagatgg ccctggtgct tctccagctt
     5881 ggcctcgaag cgttcgaaca gcgtgaaggc atcggccgtg gcaccggcga agcccgcgag
     5941 cacctggtcg cggtagagct tgcgcacctt gcgcgccgag gccttgacga cgatgttgcc
     6001 gagcgtgacc tggccgtcgc cgccgagggc gacctggcga ccgcggcgca cgctcacgat
     6061 ggtggtgccg tgataggttt ccatggggga gggaggtaag gccgcggctc gagtcttcaa
     6121 ccgggctgca gcgggtcagt ccaggctaaa tgttccggac tttgacccgg gcatgctcgt
     6181 tgatgcccat cgcgccgaag aagagcgcca ccaggcggtg ggcatcgtcg aagctgaccg
     6241 tgcggttctc gccgcgccgc gccagcaccc agttgagcag gtcgcccgca gcgatgaagt
     6301 ccacgcggat cagccgcgag caggagacac tgacgatggc ggcggcgccc agctggccct
     6361 gcagtctgtc tagcgtctgg ccgatgtcgc cgaccagctg cccggacagc tccacgctgg
     6421 cggtgggcac catgctgccg tcgtcctgct gctgggtttc gtcgaagccc gtcgacacgt
     6481 cggtgaggac ggaaacgggc gccgtcgtgg agcggccgcc ttcgctgacg cgcgccacgc
     6541 acctggcggg ctcccaggac ggcggcgaga cctcgtaggt cacgcagtag tcgatcgcca
     6601 cctcgtcgaa ctggtcggcc cggttggtca ggcgcagcgc gtccaggcgc agcagccaga
     6661 aggccgggtc ggcgtcgcgc acgccggcgg gggcgcactc ggccagtacc ccgagcaggt
     6721 gctcgccgcc cagccagcgc atgtccaggt tctgcccggc ccagccgcgc atcagcaggc
     6781 tcagctcatg gcaggcctcg gcgtcgatgc tgcgcacggc gttccagtcg agcacccagg
     6841 gcagcggcat ctgcagcgtc tgggcgcgca gtttggccac ggcctcgata tccaccatgt
     6901 cgggggcgac ccagcctatc gagccgtcga ccctggcgcc gctgcgcttg accttgtctt
     6961 ccgccgctgc tgcggcttcg gcgaccagta ggggcagcga gaaccattgc ggagccgacc
     7021 agccgaactg ctgggcatag tccagcgcca ggctctcgaa cttgtgctgc tggccgatgg
     7081 cccgatagag gtcgaacagc accagccagg tctcggcatg ctgggcgcgg ctgccgccca
     7141 tgccgatcag cgaggacagc gattgctcgc acagctcgaa gtccgcgttg gcaaaggcga
     7201 tcacggcctc gtccaactcc ggatcgtggg cgatctcgtt gacctcgacc gcgaagggat
     7261 tggcgaagtc gttggctccc agggcggcag cgaagggcga cagcggggac atgcctgcgc
     7321 ccatcacgac ggcggcgggc ggcatcgccg ccgcagcagg cgctgccggc ctcggctcgc
     7381 cacgctgccc tatatgcatg ggcgtggtgg gtgcatcgcc gatcgccgcg ggctgggtcg
     7441 gcgcgttgta gaaggaggag ggccgggccg gcgcgtggcc gcgcgcgttg aggccctcgc
     7501 cgaccatctg ctgctcgatc tcgtcgatct tggcctt
//
LOCUS       DASUCY010000600         3373 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1362923, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000600 DASUCY010000000
VERSION     DASUCY010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1362923"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VFV25_11830"
     CDS             <1..523
                     /locus_tag="VFV25_11830"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY1394057.1"
                     /translation="VGGENRVPGKSDGSSKIALWLEIAPEVKAVQHLLREAAQEEQVD
                     VELLKAMVAVESGFNAKAQSPRGALGLMQITPETAQRYATPAEARRPVRELLLDPRSN
                     LLIGARMLHDLLQRYSKIDVALAAWNAGEGTVRRAGGKMPAIAETQAHVQMVLELYWA
                     LMQRGTPHGMPNG"
     gene            567..818
                     /locus_tag="VFV25_11835"
     CDS             567..818
                     /locus_tag="VFV25_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HEY1394058.1"
                     /translation="MSTMNISLPEALKAFVDEQVAQGGYGTSSEYVRELIRKDQDRLQ
                     LRGLLLAGASSAPAAVADAGYFERLRQRVHKSAKAGTRR"
     gene            815..1147
                     /locus_tag="VFV25_11840"
     CDS             815..1147
                     /locus_tag="VFV25_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEY1394059.1"
                     /translation="MKAKPVIPREQALQDVDEAVAYYLGEGSVQAALGFIDAVEQAYT
                     LIGRHPAAGSPRYAHELNLPGLRSWSLARYPHLVFYAEREQHIDIWRVLHGHRDIPAW
                     MQAPEGES"
     gene            1213..>3373
                     /gene="parC"
                     /locus_tag="VFV25_11845"
     CDS             1213..>3373
                     /gene="parC"
                     /locus_tag="VFV25_11845"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEY1394060.1"
                     /translation="MEPDLFDTPSAPADGGDSLTLAHYAERAYLEYALSVVKGRALPD
                     VCDGQKPVQRRILYAMERMGLAFTTASGPKAVKSARVVGDVLGRFHPHGDQAAYDALV
                     RMAQDFSQRYPLIDGQGNFGSRDGDGAAAMRYTEARLSPIARLLLDEIDEGTVDFVPN
                     YDGSTQEPRQLPARLPFVLLNGASGIAVGLATEVPSHNLREVAAAAVALIRNDKLSDD
                     ELFSLLPGPDFPGGGQIISSEADIREAYATGRGSLKVRARWKIEDLARGQWQLVVTEL
                     PPGTSAQKVLEEIEELTNPKLKAGKKTLTAEQTQLKASVLAVLDAARDESNKDAPVRL
                     VFEPKSRTVEQQDFINTLLAHTSLESSAPVNLTMVGADGRPTQKSLRQILGEWIAYRL
                     ATVQRRTQHRLGKVLDRIHILEGRQLVLLNIDEVIHIIRNADEPKPALVARFKLSDRQ
                     AEDILEIRLRQLARLEAIKIEQELSALRAEQGKLEDILGSPASLKRTVVKEIEADAKL
                     HGDERRTLIQAEKKAVAEVRIVDEPVTVVVSQKGWVRARQNHGHDAASFAFKAGDGLY
                     GTFECRTVDTLLAFGSNGRVYSIAVASLPGARGDGAPVTTMIELETGTTLAHYFAGTA
                     DTTLLLANTGGFGLLAKLGDMTSRQKGGKSFLSLEPADKLLPPSAVLPAHSQVACLSL
                     GGRLLVFPLDELKLQPNGGRGLTLMDVDAKDPLVSVAS"
BASE COUNT          597 a         1131 c         1158 g          487 t
ORIGIN      
        1 tgtcggcggc gagaaccgcg tgccgggcaa gagcgacggc tcgtccaaga tcgcgctgtg
       61 gctggaaatc gcgcccgagg tcaaggcagt gcagcatctg ctgcgcgagg ccgcccagga
      121 agagcaggtg gacgtggaac tgctgaaggc aatggtggcg gtcgagtcgg gcttcaacgc
      181 caaggcgcag tcgccgcgcg gtgcgttggg cctgatgcag atcacgcccg agacggcgca
      241 gcgctacgcc acgccggcgg aggccaggcg cccggtgcgg gagctcctgc tcgatccgcg
      301 cagcaatttg ctgatcggcg cacgcatgct gcatgacctg ctgcagcgct actcgaagat
      361 cgatgttgcg ctggcggcct ggaacgccgg cgaaggcacg gtgcgccgcg ccggcggcaa
      421 gatgcctgcg attgccgaaa cccaggccca tgtgcagatg gtgctcgagc tgtactgggc
      481 cctgatgcag cggggtacgc cgcatggtat gccgaacggg tgaggagcat ttgccaaaat
      541 ttgccagaga acgtagcctt caatccatga gcacgatgaa catctccttg cccgaggccc
      601 tcaaggcctt cgtcgacgag caggtcgcgc agggcggcta tggaaccagc agcgagtacg
      661 tgcgtgagct catccgcaag gatcaggacc gactgcagct gcgcggtttg ctgcttgcgg
      721 gtgcgtcatc cgcgccggca gcggtggccg atgccggcta cttcgagcgc ctgcgtcagc
      781 gtgtgcacaa gtcggccaag gccggcacgc ggaggtgaag gccaagcctg tcatcccgcg
      841 cgagcaggcg ctgcaggacg tggatgaggc cgttgcgtac tacctcggcg aaggctcggt
      901 gcaggctgca ttgggcttca tcgatgccgt cgagcaggcg tacaccctga ttggccgcca
      961 tccagccgcc ggttcgccgc gctacgcgca tgagctgaac ctcccggggt tgcgctcctg
     1021 gtcgcttgcg cgctaccccc atctggtctt ctacgcggag cgagagcaac acatcgacat
     1081 ttggcgcgtg ctacacggac accgcgacat cccggcgtgg atgcaggcgc ccgaaggcga
     1141 gtcgtgacgc ctccgccgag cattcaacat caatagtcgg cgcgcgcagc gcgccgaaac
     1201 cgcatgcccc ccatggaacc cgatctcttt gacacgcctt cagcgcccgc cgatggcggc
     1261 gattcgctca cgctcgccca ctacgccgag cgcgcctatc tcgaatacgc gctgagcgtg
     1321 gtcaagggcc gtgcgttgcc cgatgtctgc gatggccaga agccggtgca gcgccgcatc
     1381 ctctacgcga tggagcgcat gggcctggcc ttcacgacgg cgagcggccc caaggcagtg
     1441 aaaagcgcgc gcgtggtcgg cgatgtgctg ggccgcttcc acccgcatgg cgaccaggcc
     1501 gcctacgacg cgctggtacg catggcgcag gacttctcgc agcgctaccc gctgatcgac
     1561 ggccagggca acttcggcag ccgcgacggc gacggcgccg cggcgatgcg ctacaccgag
     1621 gcgcggctga gccccatcgc gcggctgctg ctcgacgaga tcgacgaagg cacggtggac
     1681 ttcgtgccca actacgacgg ctccacgcag gagcccaggc agctgcccgc gcgtctgccc
     1741 ttcgtgctgc tcaacggcgc gtccggcatt gccgtgggcc tggcgaccga agtgcccagc
     1801 cacaacctgc gcgaggtggc cgccgccgcg gtggcgctga tcaggaacga caagctgtca
     1861 gacgacgagc tgttctcgtt gctgcccggc cccgacttcc cgggcggcgg ccagatcatc
     1921 agcagcgagg ccgacatccg cgaggcttat gcgaccggcc gcggcagcct caaggtgcgc
     1981 gcgcgctgga agatcgaaga cctcgcgcgc ggccagtggc agctcgtcgt caccgagctg
     2041 ccgcccggca ccagcgcaca gaaggtgctg gaagagatcg aggagctcac caaccccaag
     2101 ctcaaggccg gcaagaagac gctcaccgcc gagcagaccc agctcaaggc cagcgtgctg
     2161 gcggtgctgg acgccgcgcg cgacgaatcg aacaaggacg ccccggtgcg cctggtgttc
     2221 gagcccaaga gccgcactgt cgagcagcaa gacttcatca acacgctgct ggcgcacacc
     2281 agcctggaaa gctcggcgcc ggtcaatcta acgatggtgg gtgcggatgg ccggcccacg
     2341 cagaagagcc tgcgccagat cttgggcgag tggatcgcct atcggctggc caccgtgcag
     2401 cggcgcaccc agcaccggct gggcaaggtg ctggaccgca tccacatcct cgaaggccgg
     2461 cagctggtgc tgctgaacat cgacgaggtg atccacatca tccgcaatgc cgatgagccc
     2521 aagccggcgc tggtcgcgcg cttcaagctc agcgatcggc aggccgagga catcctcgag
     2581 attcgtctgc gtcagctggc ccggctggag gctatcaaga tcgagcagga gctttcagcc
     2641 ctgcgcgccg agcagggcaa gctcgaagac atcctcggca gcccggcgag cctgaagcgc
     2701 acggtggtca aggagatcga ggccgatgcc aagctgcacg gcgacgagcg ccgcacgctg
     2761 atccaggccg agaagaaggc ggtggccgag gtccggatcg tcgacgagcc ggtcaccgtg
     2821 gtggtgagcc agaagggctg ggtgcgcgca cgccagaacc acggccacga tgcggcctcg
     2881 tttgccttca aggccggcga cggcctctac ggcaccttcg aatgccgcac ggtcgacacg
     2941 ctgctggcct tcggctccaa cggccgggtc tacagcatcg cggtggccag cctgcccgga
     3001 gcgcgcggcg acggcgcgcc cgtcacgacg atgatcgagc tggaaaccgg tacgacgctg
     3061 gcccattact tcgccggaac ggccgatacg acgctgctgc tcgccaacac cggcggcttc
     3121 ggcctcctgg ccaagttggg cgacatgacg agccggcaaa aggggggcaa gagcttcctc
     3181 agcctggagc cggccgacaa gctgttgccg ccgtccgccg tgctgcccgc gcacagccag
     3241 gtggcttgcc tgagcctggg cgggcggctg ctcgtgttcc cgctcgacga gctcaagctg
     3301 cagcccaacg gcgggcgcgg cctgacgctg atggacgtgg acgccaagga cccgctggtg
     3361 tcggtggcga gca
//
LOCUS       DASUCY010000601         4687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1475665, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000601 DASUCY010000000
VERSION     DASUCY010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4687
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1475665"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /gene="panB"
                     /locus_tag="VFV25_11850"
     CDS             <1..739
                     /gene="panB"
                     /locus_tag="VFV25_11850"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEY1394061.1"
                     /translation="LTSYDASFAALADEAGVDSLLVGDSLGMVLQGRPSTVPVTLDEM
                     VYHTRSVARGNKAAWLIGDLPFGSYHEGRKQALRSSVALMQAGAQMVKLEGGGWTTDI
                     VHFLTERGIPVCAHLGLTPQSVHALGGYRIQGRDEAGAATLRRHAKELAEAGAAMLVL
                     ELMPSSVAAAVQADNPQLITIGIGAGPGTAGQVLVLHDMLGVTRGKLPRFVRNFMAEG
                     GDIEAALRRYIAAVKDGSFPDNKIHSY"
     gene            829..1683
                     /gene="panC"
                     /locus_tag="VFV25_11855"
     CDS             829..1683
                     /gene="panC"
                     /locus_tag="VFV25_11855"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEY1394062.1"
                     /translation="MRIVHTIADLRSALAGAARTAFVPTMGNLHEGHLSLLKLAREHG
                     GPVVVSIFVNRLQFAPHEDFDSYPRTLERDCELLLAVGCNVVFAPSEAELYPEPQTYK
                     VQPPSELADILEGQFRPGFFTGVCTVVTKLLNCVSPKVAVFGKKDYQQLMVVRRLVQQ
                     LALPTEVVAGDTARAEDGLALSSRNGYLSEAERAEAVQLSMALKDLIAHHKAALMIDQ
                     TPDLPALEAKAMDKLRARGWQPDYLSLRRRSDLQWPTAEQLRGGEPLVALGAAKLGKT
                     RLIDNLEC"
     gene            1704..2609
                     /gene="xerD"
                     /locus_tag="VFV25_11860"
     CDS             1704..2609
                     /gene="xerD"
                     /locus_tag="VFV25_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEY1394063.1"
                     /translation="MTTPREASQSSIDRFLDALWLEDGLSANTLAAYRRDLSLYADWL
                     ASKQGRAIDSSTEPDLLGYSLHRHKDTRATTSNRRLTVFKRYFRWALREHLLSADPTL
                     KLQSAKQPLRVPKTLSEAQVEALLAAPDTETPLGLRDRAMLELMYASGLRVSELVTLK
                     SVHLSLDEGALRVTGKGGKERLVPFGEEANAWLRRYLTEARAAILAGQASDAYFVTAR
                     GGGMTRQMFWTLIKKHARAADIHTPLSPHTLRHAFATHLLNHGADLRAVQMLLGHADI
                     STTQIYTHVARERLKALHAQHHPRG"
     gene            3021..3497
                     /locus_tag="VFV25_11865"
     CDS             3021..3497
                     /locus_tag="VFV25_11865"
                     /inference="COORDINATES: protein motif:HMM:NF016178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEY1394064.1"
                     /translation="MQPLPSVSAILERLITRGLQLPPGRVLVGRYGDSPELSAKLLAH
                     IREGRKRAGASLLWAWEAENESVPGVGDIEVVVDHQNEPVIVTRIAQVDVLPYGAVSA
                     EYAAIEGEGDSSLRYWREAHWAFFSRECTRLGYEPSENMPVVCCVFEVLHVSTAAN"
     gene            3764..4030
                     /locus_tag="VFV25_11870"
     CDS             3764..4030
                     /locus_tag="VFV25_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HEY1394065.1"
                     /translation="MGMNVNLTPQLEEMVRHKVDSGLYTSASEVVREALRLMEERDRL
                     REAKLEQLRQDIREGLESGPAQVRDPERVKREGRARRAAKARKA"
     gene            4133..4525
                     /locus_tag="VFV25_11875"
     CDS             4133..4525
                     /locus_tag="VFV25_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY1394066.1"
                     /translation="MSNTPIALWHQLVRTRDAAGLDDLLAEDAVLYSPVVHTPQRGKA
                     ITKKYLAAAFVVFFDPSFRYVRELVGERDAMLEFEVEIEGISVNGVDMIKWNDAGRIT
                     EFKVMLRPLKAVNLIHQKMAAMLQAGAA"
     gene            complement(4563..>4687)
                     /locus_tag="VFV25_11880"
     CDS             complement(4563..>4687)
                     /locus_tag="VFV25_11880"
                     /inference="COORDINATES: protein motif:HMM:NF013377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HEY1394067.1"
                     /translation="KYDVAQDRPGGGGEYLAQDGFGWTNGVFVAFKEGLLCPLA"
BASE COUNT          846 a         1565 c         1522 g          754 t
ORIGIN      
        1 gctgacgtcc tacgacgcca gcttcgcggc gctggccgac gaggcgggcg tcgacagcct
       61 cttggtcggc gactcgctgg gcatggtgct gcaggggcgg cccagcacgg tgccggtgac
      121 gctggacgag atggtctatc acacgcgcag cgtggcgcgc ggcaacaagg cggcctggct
      181 gatcggcgac ctgcccttcg gcagctacca cgaagggcgc aagcaggccc tgcgctccag
      241 cgttgcgctg atgcaggccg gcgcgcagat ggtcaagctc gaaggcggcg gctggaccac
      301 cgacatcgtg cacttcctca ccgagcgcgg catcccggtc tgcgcccacc tgggcctcac
      361 gccgcagagc gtgcatgcgc tgggcggcta ccgcatccag ggcagggacg aagccggcgc
      421 cgcgacgctg cgccgccatg ccaaggagct ggccgaagcg ggcgctgcga tgctggtgct
      481 ggagctgatg ccctcgagcg tcgccgccgc ggtgcaggcc gacaacccgc agctgatcac
      541 aataggcata ggcgccgggc caggcacggc cggccaggtg ctggtgttgc acgacatgct
      601 cggcgtcacg cgcggcaagc tgccgcgctt cgtgcgcaac ttcatggccg aaggcggcga
      661 tatcgaggcc gcactgcgcc gctatattgc cgccgtcaag gacggcagtt tccccgacaa
      721 caagattcac agctactagg tactactaaa aacccgccgc ttcgcttcag gccccagaga
      781 gggccaggaa atcccgggag cggccttaga ttcttgagag gctcgctctt gcgtatcgtc
      841 cacaccatcg ccgacctccg gtccgccctc gccggcgcgg ccaggaccgc ctttgtgccc
      901 accatgggca atctgcacga gggccatctg tcgctgctca agctcgccag ggagcatggc
      961 gggccggtgg tggtcagcat cttcgtcaac cgcctgcagt tcgcgccgca cgaggacttc
     1021 gacagctacc cgcgcaccct ggagcgcgac tgcgaattgc tgctcgccgt gggctgcaat
     1081 gtcgtgttcg cgcccagcga ggccgagctc tatcccgagc cgcagaccta caaggtgcag
     1141 ccgccctctg agctggccga catcctggaa ggccagttcc gccccggctt cttcaccggc
     1201 gtgtgcacgg tggtgaccaa gctcctcaac tgcgttagcc ccaaggtcgc ggtgttcggc
     1261 aaaaaggact accagcagct gatggtggta cgccgcttgg tgcagcagct cgccctgccc
     1321 accgaggtgg tggccggcga caccgcgcgc gccgaggacg gcctggccct gtcctcgcgc
     1381 aacggctacc tgagcgaggc cgagcgcgcc gaggcggtcc agctttcgat ggccctgaag
     1441 gatctgatcg cccaccacaa ggcggccctg atgatcgacc agacgccaga cctgcccgcg
     1501 ctggaggcca aggcgatgga caagctgcgc gcgcgcggct ggcagcccga ctatctcagc
     1561 ctgcgccgcc gcagcgacct gcagtggccc accgccgagc agctgcgcgg cggcgaaccg
     1621 ctggtggcgc tgggcgccgc caagctcggc aagacgcggc tgatcgacaa cctggaatgc
     1681 tgatcggcta gcgaccagtt cggatgacga cgccgcgcga agccagccag tcgtcgatcg
     1741 accgcttcct cgacgcgctc tggctggaag acggcctgag cgccaacacc ctggccgcct
     1801 accggcgcga cctgagcctc tatgccgact ggctggcaag caagcaaggc cgcgccatcg
     1861 acagcagcac cgagcccgac ctgctcggct acagcctgca tcgccacaag gacacgcgcg
     1921 ccaccacctc caaccgccgc ctcacggtct tcaagcgcta cttccgctgg gccctgcgcg
     1981 agcacctgct gagtgccgac cccacgctca agctgcaatc ggccaagcag ccgctgcgcg
     2041 tgcccaagac cctgagcgag gcccaggtcg aggccctgct cgctgcaccg gataccgaga
     2101 cgccgctggg cctgcgcgac cgcgcgatgc tggaactgat gtatgcgagc ggcctgcgcg
     2161 tgagcgaact cgtcacgctc aagagcgtgc acctgagcct ggacgaaggc gcgctgcgcg
     2221 tgaccggcaa gggcggcaag gaacgcctcg tgcccttcgg cgaggaagcc aacgcctggc
     2281 tgcgccgcta cctgaccgaa gcacgtgccg ccatcctcgc cggccaggcc agcgatgcct
     2341 acttcgtgac cgcacgcggc ggcggcatga cgcggcaaat gttctggacc ctgatcaaga
     2401 agcacgcccg cgccgccgac atccacacgc cgctgtcgcc gcacacgctg cgccacgcct
     2461 tcgccaccca tctgctgaac cacggcgccg acctgcgcgc ggtgcagatg ctgctcggcc
     2521 atgcggacat ctcgaccacg cagatctaca cccacgtcgc gcgtgagcgc ttgaaggcgc
     2581 tgcatgcgca gcatcatccg cgggggtgat gtgggcgcct cgcgccttcg gcgcgggata
     2641 caggcatgag cggtcgcgtg gtgagatggg gaaatgcccg tggaatggag cgactagacc
     2701 tggccgcaat cggaccacgg tgactgtccg cagacggaca cctaagatgg tgcgcgtgaa
     2761 atccgcaaca gcttttggag atctaatgcg cttctcgctg caaacgcgcc aacgattcgc
     2821 cgttgttttg ggcttcagct tgttcatcgc ggtttcgctc gttgcgggtt actttgcggc
     2881 cggggttgcc acacccgaac aggcgtgtga aaggcgctgc gcaaaggtca atagggtggg
     2941 gcaactcgtg tatcacggtc cgcggacgcc gaaggacacg cgatacgtca gcgaatgcga
     3001 atgccgatag gcagctccaa atgcagccac tcccttctgt gtccgcaata cttgagcggc
     3061 tcattacccg cgggcttcag ctgccgcctg gtagggtact cgttggtagg tacggcgact
     3121 ccccggaact ctcggcgaag cttctcgcgc acattcgcga aggccgaaaa cgcgccggag
     3181 ccagcctcct ttgggcctgg gaggcggaga acgaatcggt tccgggagtc ggcgacattg
     3241 aagtggtcgt tgatcaccag aacgagcccg tcatcgttac gcgcatagcg caggtcgacg
     3301 tgctgcctta tggcgctgtc agcgccgagt acgctgccat tgaaggggaa ggtgacagct
     3361 cgcttaggta ctggcgcgag gcgcattggg cgttcttctc gcgcgagtgc acgcgcctcg
     3421 gttatgagcc gtccgaaaac atgccagtcg tgtgttgcgt gtttgaggtc cttcacgtgt
     3481 caacggctgc caactgattc gtatcaatca cgaagagcgc tagagggccc cttctcactc
     3541 gccacggggg cgaaagacgc ggtctccact cgcccgtcgt ccacacgccg tagcgcggca
     3601 aagcgatcac gaagaaatac ctggcggcgg ccttcgccgt gttcttcaat ccgtccttcc
     3661 gctatgtttg agcccgatca aatgtcaatc tttgacatct tttgatagga taaacaaggg
     3721 caggcagtgc ctgcaaagcg atgtggcgaa gcgaggtgcc cccatgggta tgaacgtcaa
     3781 tctcacgccg cagctggagg aaatggtgcg gcacaaggtc gactcgggtc tgtacacgtc
     3841 ggccagcgag gtcgttcgtg aggcgctgcg cctcatggaa gagcgggatc gcctgcgcga
     3901 ggccaagctc gagcagctga ggcaagacat ccgtgagggc ctggaaagcg gcccagccca
     3961 ggtgcgggac ccagagcgtg tcaagcgcga agggcgcgcc aggcgcgccg ctaaggcgcg
     4021 caaggcttga gatgccgcgc atcgatcatg gcctcgcgtt cgtccatggc gcagtagcct
     4081 tccggcacct cggcaatgtc tccaagccgg acttctcgta cgggagcaca tcttgagcaa
     4141 cacacccatc gccctttggc accaactggt ccgcactcgc gacgccgccg gccttgatga
     4201 cttgctggcc gaagacgcgg tcttgtactc gcccgtcgtc cacacgccgc aacgcggcaa
     4261 ggcgatcacg aagaagtacc tggcggcggc cttcgtcgtg ttcttcgatc cgtctttccg
     4321 ctatgtacgc gagctggtcg gcgaacgcga tgcgatgctg gaattcgagg tcgagatcga
     4381 gggtatcagc gtcaacggcg tcgacatgat caagtggaac gacgcgggcc gcatcacgga
     4441 gttcaaggtc atgctgcgcc cgctcaaggc agtgaacctg atccaccaga agatggcggc
     4501 gatgctgcag gccggcgcgg cctagcgccg ggcagtgcgc aacgcaaagg cgggcggcgc
     4561 gctcaggcga gcgggcacag caggccctcc ttgaatgcaa cgaagacgcc gttggtccag
     4621 ccgaagccgt cctgggccag atactcgccg ccgccgccgg ggcgatcctg ggcgacatcg
     4681 tatttct
//
LOCUS       DASUCY010000602         5711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1106179, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000602 DASUCY010000000
VERSION     DASUCY010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 5711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5711
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106179"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..123
                     /locus_tag="VFV25_11885"
     CDS             <1..123
                     /locus_tag="VFV25_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY1394068.1"
                     /translation="APPNGIYAVFPQRKHLPLRVRLWIDFLKHSYGDADYWSAH"
     gene            complement(205..537)
                     /locus_tag="VFV25_11890"
     CDS             complement(205..537)
                     /locus_tag="VFV25_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HEY1394069.1"
                     /translation="MKQLLTALALTVAFAGSAFAADAASAPAEKPMSSQQEKMKMCNK
                     DATGKKGDERKAFMKDCLSKEHEAKAADAKADAKMNQQEKMKMCNKDATGKKGDERKA
                     FMKECLSK"
     gene            complement(775..2109)
                     /locus_tag="VFV25_11895"
     CDS             complement(775..2109)
                     /locus_tag="VFV25_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEY1394070.1"
                     /translation="MNAPIPPPTPEQIGKYRVIRRLGEGATSEVFLCRDDFHTRDVAL
                     KRVRAGALDDSQEGHAHARFFAAEAALVGRLQHPNVVQIFDAVADPVEPYVVMEYVAG
                     TTLRPYCRPDQLLTLELIVEIGFKCAMALGYVFRQGLIHRDVKPANVLAVLINGSITD
                     IKLTDFGSALNLASDATQVFRVGSLAYMSPEQLDGGLISAQADIYSLGAVLYHLIAGR
                     APFEAPQQSAMMNQIYTQAPAPLTGLRAGVTEALDAVIQKALAKQPQARYANWDEFAQ
                     ALSGLIANHQVPRGQLQEVLDSERFNLLRKLDFFFSFGDVELWEVVHRAKWQRFPLNF
                     AIYRRGEEGNNFHIIAEGSVEVIRDGTRVAQLGAGTSVGEMAYLAPSPDLKRHSTDVL
                     VTEPVTAVSFTPDSLAHLSPNCRHLFDEAFIRVLVRRLHVAHEALAHPRRIL"
     gene            2265..3950
                     /gene="argS"
                     /locus_tag="VFV25_11900"
     CDS             2265..3950
                     /gene="argS"
                     /locus_tag="VFV25_11900"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEY1394071.1"
                     /translation="MIRAKQELLAALGEALAELAPESQISPSFENPKQAAHGDLAITA
                     AMQLAKPMKRNPRELAQGLIDALQRKPAAQRWVDAMEIAGPGFINLRLKAAAKQAIVA
                     DVLAAADAFGRRPAHGGKLMVEFVSANPTGPLHVGHGRQGALGDSISHLFETQGWQVT
                     REFYYNDAGVQIATLAASTQARIKGLKPGDAGWPENAYNGDYIADIAADFLAKKTVKA
                     DDREFTASGDPEDLDGIRQFAVAYLRHEQDLDLQAFGVSFDNYYLESSLYTGGRVERT
                     VSSLAAAGKTYESDGALWLRSTDYGDDKDRVMRKSDGSYTYFVPDVAYHVSKWERGYT
                     KVINCQGTDHHGTIARVRAGLQGLGLGIPEGYPDYVLNTMVRVMKGGEEVKISKRTGG
                     YVTLRDLIEWTSRDAVRFFLISRKADTEFTFDVDLALKRNDENPVYYVQYAHARICSV
                     LQQYVEQKQGDLAALANADLSRLVAPAEAALMLKLAEYPDMLSSAARELAPHDVAFYL
                     RELASAFHTYYNAERFLVDDAALACARLALLQATAQVLRNGLALLGVSAPEKM"
     gene            4002..4622
                     /locus_tag="VFV25_11905"
     CDS             4002..4622
                     /locus_tag="VFV25_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEY1394072.1"
                     /translation="MKSQRGGFFVGMIVGLLIGLALALAVALYVTKAPVPFVNKVPQR
                     SPEQDAAEAAKNKNWDPNAPLAGKNPARPASVPEAAPSSDAAPAAQPSTAPSVTAGKP
                     ANKEAAAAAPADKSAAPSTAAAPDAFVYFVQAGAYSHTEEAEAQRAKLAMLGLQAKIT
                     EREQNGRTVYRVRVGPYEQRTDAEAAKDKIDGNGIGDTALVRVQKP"
     gene            4706..5362
                     /locus_tag="VFV25_11910"
     CDS             4706..5362
                     /locus_tag="VFV25_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEY1394073.1"
                     /translation="MKRREFSSLAMGLGVAGLGLASPVARAQGAAVPVEGKNYVRLSQ
                     PQPTLAPPGKIEVVEFFWYGCPHCFHFDPTLQAWVAKLPADVTFRRVHVGFQGIHKIH
                     QRLFYALEIMGREAELHGKIFNAIHVQRNMLDKPETIADFVAANGIDRTKFLEVFNSF
                     GAQTRCQQASRLVEGYKIDGVPTMGVAGRYFTEGSLAGTNEAALQIVDFLLDRIRKGH
                     "
     gene            5506..>5711
                     /locus_tag="VFV25_11915"
     CDS             5506..>5711
                     /locus_tag="VFV25_11915"
                     /inference="COORDINATES: protein motif:HMM:NF015901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HEY1394074.1"
                     /translation="MRGRLAALLGALLLAAALPAAQAEKGDRDEQLNYAGDSGHADLA
                     KQVYVLKGNVVLTKGSIVIKADEA"
BASE COUNT          993 a         1874 c         1858 g          986 t
ORIGIN      
        1 gcgccgccca acggcatcta cgcggtgttt ccgcagcgca agcatctgcc gctgcgcgtg
       61 cggctatgga tcgacttcct gaagcacagc tacggcgacg cggactactg gtcggcgcac
      121 tgagccgcga agaaaagaaa agcccggcgg tgccgggctg tatcgcggag ttcaacctgc
      181 ggccgcgctc cctttttctc attcttactt gctcaggcat tccttcatga aggccttgcg
      241 ctcgtcgccc ttcttgccgg tggcgtcctt gttgcacatc ttcatcttct cctgctgatt
      301 catcttggca tcagccttgg catcggcggc cttggcctcg tgctccttgc tcaggcaatc
      361 cttcatgaag gccttgcgct cgtcgccctt cttgccggtg gcgtccttgt tgcacatctt
      421 catcttttcc tgctgcgagc tcatgggctt ttcggcagga gcgctggcag cgtcagcggc
      481 gaaggcggag ccggcaaaag cgacggtgag agccagggca gtcaacagtt gtttcatgga
      541 gtctccttgg gtggcaagat cgccggccag cattgaagca cggcgcttct agcctcggca
      601 agccgtcaat gcccgagggc gccgcgcggt tgacaggcag attagggatc gttgcgctgc
      661 cggcttgcgc gacccgggta ttgccaagcg gccgccgcgg attccggctt tgccgggccg
      721 ctggcggcgc ccccttctat gaggaccggt ttgtcaaggc gatccggggg tggtctacaa
      781 aatgcggcgg ggatgggcga gtgcttcatg agccacgtgc aggcgccgca ccagcacgcg
      841 gatgaaggcc tcgtcgaaga gatggcggca attggggctg aggtgggcca ggctgtcggg
      901 cgtgaaggac acggccgtca ccggctcggt gaccagcaca tcggtgctgt gcctcttgag
      961 atccgggctg ggcgccaggt aggccatctc tcccaccgag gtgccggcgc cgagctgtgc
     1021 gacccgcgtg ccgtcgcgga tgacttccac cgaaccctcg gcgatgatgt ggaagttgtt
     1081 gccctcctcg ccgcgcctgt agatcgcgaa attgaggggg aagcgttgcc atttcgcgcg
     1141 gtgtacgact tcccacaact ccacgtcgcc gaagctgaag aagaaatcga gcttgcgcag
     1201 caggttgaag cgctccgaat cgagcacctc ctgcaactgc ccgcgcggca cttggtggtt
     1261 ggcgatcagt cccgacaagg cctgcgcgaa ctcgtcccag ttcgcgtagc gcgcctgcgg
     1321 ctgcttggcc aaggccttct ggatcactgc gtccagcgcc tcagtgacgc cggcgcgcag
     1381 cccggtgagc ggtgccggtg cctgggtgta gatctggttc atcatcgccg actgctgcgg
     1441 cgcctcgaag ggcgcgcgtc cggcgatcag gtgatagagc acggcgccca gcgaatagat
     1501 gtcggcctgg gcagagatca ggccgccgtc gagctgttcg ggcgacatat aggccagcga
     1561 gccgacgcgg aatacctggg tcgcgtcgga ggccagattg agcgcgctgc cgaagtcggt
     1621 cagcttgatg tcggtgatgc tgccgttgat cagcacggcc agcacattgg ccggcttcac
     1681 gtcgcggtgg atcaggccct ggcggaacac atagcccagc gccatggcgc acttgaagcc
     1741 gatttcgacg atcagctcca gcgtcagcag ctgatccggc ctgcagtaag gccgcagcgt
     1801 ggtgccggcc acatactcca tcacgacata gggctcgacc ggatcggcca cggcatcgaa
     1861 gatctgcacc acattggggt gctgcagcct gccgaccagg gccgcctcgg cagcgaagaa
     1921 gcgggcgtgg gcgtgcccct cctgcgagtc gtccagcgct ccagcgcgca cccgcttgag
     1981 tgccacgtcg cgcgtgtgga agtcatcgcg gcagagaaag acctcgctgg tggctccctc
     2041 gccgagacgg cggatcacgc ggtacttgcc gatctgctcc ggggtgggcg ggggaatggg
     2101 ggcattcatt ctgttgtatg ggctgcgagc cggcctatct ttccatcgac ccggcccgtg
     2161 cacaagctga agtccgcgag tatggcttgc agagatgtca ggcccgtgca cagatggcgg
     2221 tgcggcgccg ggcagcaaga cccccaacct tagaatcgcc gaccatgatc cgcgccaagc
     2281 aagagctcct ggcggccctg ggcgaggccc tggccgaact ggcccccgag agccagattt
     2341 ccccctcctt cgagaacccc aagcaggcgg cccatggcga tctggccatc accgccgcga
     2401 tgcaactggc caagcccatg aagcgcaatc cgcgcgaact ggcgcagggc ctgatcgatg
     2461 ccctgcagcg caagcccgcc gcgcagcgct gggtcgacgc gatggaaatc gccggccccg
     2521 gcttcatcaa cctgcgcctg aaggccgcgg ccaagcaggc catcgtggcc gatgtgctgg
     2581 ccgccgctga tgccttcggc cgcaggcctg cgcatggcgg caagctgatg gtcgagttcg
     2641 tgtcggccaa tcccacgggc ccgctgcatg tgggccatgg tcgccagggc gctttaggcg
     2701 actcgatcag ccacctgttc gagacccagg gctggcaggt cacgcgcgag ttctattaca
     2761 acgatgccgg cgtgcagatc gccacgctgg ccgcctccac acaagcacgc atcaagggtc
     2821 tgaagccggg cgatgccggc tggcccgaga acgcctacaa cggcgactac attgccgaca
     2881 tcgcggccga cttcctcgcg aagaagactg tgaaagccga cgaccgcgag ttcaccgcct
     2941 cgggcgatcc ggaagacctg gacggcatcc gccagttcgc ggtggcctat ctgcgccacg
     3001 agcaggacct ggacctgcag gccttcggcg tcagcttcga caactactac ctcgagtcca
     3061 gcctctacac cggcgggcgc gtcgagcgga ccgtgtcttc gctggcggcg gcaggcaaga
     3121 cctacgagag cgatggcgca ctgtggctga gatccaccga ctacggcgac gacaaggacc
     3181 gcgtgatgcg caagtccgac ggctcctaca cttatttcgt gcctgacgtg gcctatcacg
     3241 tcagcaagtg ggagcgcggc tacaccaagg ttatcaactg ccagggtacc gatcaccacg
     3301 gcaccatcgc ccgcgtgcgc gccggcctgc agggcctggg cctgggcatc cccgagggct
     3361 accccgacta cgtgctcaat accatggtgc gcgtgatgaa gggcggcgag gaggtcaaga
     3421 tctccaagcg caccggcggc tatgtcacgc tgcgcgacct gatcgagtgg accagccgcg
     3481 acgcagtgcg cttcttcctt atcagccgca aggccgacac cgaattcacc ttcgacgtgg
     3541 acctggcgct caagcgcaac gacgagaacc cggtctacta cgtgcagtac gcccatgcgc
     3601 gcatctgctc ggtgctgcag caatacgtcg agcagaagca aggcgatctc gccgcgctgg
     3661 cgaacgccga cctgagccgg ctcgtcgcgc cggccgaggc cgcgctgatg ctcaagctgg
     3721 ccgagtatcc cgacatgctg agcagcgccg cgcgcgagct ggcgccgcat gacgtggcct
     3781 tctatctgcg cgagctggcc agcgcctttc acacttatta caacgccgag cgctttctcg
     3841 tcgatgatgc agccctggca tgcgcgcggc tggcgctgct gcaggccacg gcccaggtgc
     3901 tgcgcaatgg cctggcactg ctgggcgtga gcgcgcccga gaagatgtag ggcttgtcga
     3961 aatcaccagc cctgaagctt ttgttcggag acttttccca catgaaatct caacgcggtg
     4021 gattcttcgt cggcatgatc gtcggcctac tgatcggctt ggcgctggcg ctggcggtcg
     4081 ctctgtacgt gacgaaggcg ccggtgccct tcgtcaacaa ggtgccccag cgcagtcccg
     4141 agcaggatgc ggccgaggcc gcgaagaaca agaactggga ccccaatgcg ccgctggccg
     4201 gcaagaaccc ggcgcgcccg gcctcggtgc ccgaggcagc gccgtcatcg gatgccgccc
     4261 cagcggccca gccctcaacc gcgcccagcg tcaccgcggg caagcccgcg aacaaggagg
     4321 ccgcggctgc tgcgccagct gacaaatcgg ccgccccgtc caccgccgcg gcccccgatg
     4381 cgtttgtcta tttcgtacag gccggcgcct attcgcatac cgaggaggcc gaggcccagc
     4441 gcgccaagct ggcgatgctg gggctgcagg ccaagatcac tgagcgcgag cagaacgggc
     4501 gtacggtcta tcgggtgcga gtggggcctt atgagcagcg tacggacgcc gaggcggcga
     4561 aggacaagat cgacggcaat ggcatcggcg ataccgcgct ggtgcgcgtc cagaagccgt
     4621 aagcctcagg cttgaacttt tgccgcgatt gcaggctcca ccacggcttc gcaatggaat
     4681 gaagatcaac acggaaggaa gatcgatgaa gcgtcgcgag ttttcgtccc tggcgatggg
     4741 cctgggcgtt gcgggcctcg gccttgcgag cccggtggcg cgcgcccagg gagccgccgt
     4801 gccggtggaa ggcaagaact atgtgcgcct gtcccagccc cagccgacgc tggcaccgcc
     4861 cggcaagatc gaggtcgtcg agttcttctg gtacggctgc ccgcattgct tccacttcga
     4921 tccgacgctg caggcctggg tggcgaagct gccggccgat gtcaccttcc gccgcgtgca
     4981 cgtaggcttc cagggcatac acaagatcca ccagcgcctg ttttacgcgc tggagatcat
     5041 gggccgcgag gccgagctgc acggcaagat cttcaatgcc atccacgtcc agcgcaacat
     5101 gctggacaag cccgagacca ttgccgattt cgtcgccgcc aacggcatcg atcgaaccaa
     5161 gttcctcgag gtcttcaatt ccttcggcgc acagacccgc tgccagcagg cttcgcgcct
     5221 cgtcgagggc tacaagatcg acggcgtgcc gaccatgggc gtggcgggcc gctatttcac
     5281 cgagggctcg ctggccggca ccaacgaggc ggcgctgcag atagtcgatt tcctgctcga
     5341 tcgcatacgc aagggacatt gagcaggcgt aggctgacat cgatgtaaag gcgcccgcgg
     5401 gcgccttttt tcgtggctag aatcctctgc aatttccatg ccgccatgtc tttgcaccac
     5461 ccacgcctcc catccgcttc ccctgtaagt cctgggagcc ggagtttgcg gggccggttg
     5521 gcggcgctgc tcggcgcgct cctgctggct gcggccttgc cggctgcgca ggccgagaag
     5581 ggcgatcgcg acgagcagct gaactatgcc ggcgactccg gccatgccga cctcgccaag
     5641 caggtctacg tgctcaaggg caatgtggtg ctgaccaagg gcagcatcgt catcaaggcc
     5701 gacgaggccg a
//
LOCUS       DASUCY010000603         3927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_752677, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000603 DASUCY010000000
VERSION     DASUCY010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752677"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="VFV25_11920"
     CDS             complement(<1..631)
                     /locus_tag="VFV25_11920"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY1394075.1"
                     /translation="MKHLLLLGDGPAHLHVLKSLGQERLAVGRVSLVAPSAEPLCAAM
                     LPRWVAGRASEERCRLPLAALARAAGADFIEASVQSLDLAARSLQLSSGSAIGYDALS
                     LDIAPEIAPEALPGARELGLFVHPLQHFSQLWPRLRALAEARPLCIVVIGGDVLAVEL
                     AFALERALGRDSRISLVSGSGGLLPDFAERARHRVQDKLRQRRITLFGED"
     gene            complement(652..1404)
                     /locus_tag="VFV25_11925"
     CDS             complement(652..1404)
                     /locus_tag="VFV25_11925"
                     /inference="COORDINATES: protein motif:HMM:NF022694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HEY1394076.1"
                     /translation="MSAARIVPGWRHAVLGVLCALGWQGAAAAGNALAPFAMVGGEPV
                     PPWRWSLLPHQTKPVTRFSVGDFEGKHALRVEADKSYGNLAQDLHFTAGAAPPHLLSW
                     QWRVEELNMASDLRKRSGDDTTLKLCAMFELPLDRVPFVERQLLRLMRARSSEPMPSA
                     SVCYVWDAHLSAGTELPNAFTKRVRYIVLESGAAHLHQWVAERRDVAADFLRLFGDES
                     QVVPALVGVAVGADADNTQQRSLAYVAELALE"
     gene            complement(1436..1966)
                     /locus_tag="VFV25_11930"
     CDS             complement(1436..1966)
                     /locus_tag="VFV25_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY1394077.1"
                     /translation="MLEHYRLTARYNRWMNERLYAAASGLSDAERKRDRGAFFGSLHN
                     TLAHLVLADKVWLNRFAALDKRFAALTPAVLAMPSFTGLDQVLFDDFEAMRAHRAEMD
                     AAIEAWTAELTPDDVTRLMRYATSKGVPREHEAWYAFSHFFNHQTHHRGQATTLLMQA
                     GVDPGVTDLIAMLPQQ"
     gene            complement(2003..2461)
                     /locus_tag="VFV25_11935"
     CDS             complement(2003..2461)
                     /locus_tag="VFV25_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEY1394078.1"
                     /translation="MMSRLLRQALGVTVWIDRLQPVALLAMRLYVAQVFFNSGLAKLG
                     NWHGTLALFHYEYHVPLLPPDVAAYSGAFGELVFPVLIALGLAGRFAAAALFVVNLVA
                     VVSYPQLFEFECPAPVNSHFYWGSLLALLTVFGPGKLALDTLVLRRLSTR"
     gene            complement(2458..3258)
                     /locus_tag="VFV25_11940"
     CDS             complement(2458..3258)
                     /locus_tag="VFV25_11940"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HEY1394079.1"
                     /translation="MAERPTALLQGQRAFAAALRDAAQTEPALHLFKGEQALVQRRLA
                     IYRGNAVGAVAKALAAAYPVIVQIVGEEFFGGLARAHLRECPSTSGDLYDYGAGFAQF
                     LAQFPPVAEFAYLPDLARLEWAAHRAYAAADAPLFDLQALARVPAAQQAGLRFQLAPG
                     TALIRSAHPIVRLWTIHQDGYDGEFSVDWDRAETALVTRRGLDVLVSAVGPGMAMFLA
                     GLLGGASLERAARLASDADAGFELAAALGAAVRANLICGFASPEGANT"
     gene            complement(3251..>3927)
                     /locus_tag="VFV25_11945"
     CDS             complement(3251..>3927)
                     /locus_tag="VFV25_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HEY1394080.1"
                     /translation="AYPISLHGVGLSLGSADALAQRHLAKLKQLVDRVEPALVSEHLC
                     WGALDGVHFNDLLPLPYTEEALRLLADRVARVQDTLRRTILVENLSAYVEYRGAEMSE
                     TSFLAELSRRTGCGVLFDVNNLYVNAVNFGGDALALMAELPGSAIGQIHLAGHLRTDD
                     GLIDHHGSRVSPDVWRLYEQACARFGPKPTLIEWDTDLPALSVLLEEARLARELQSAL
                     LEPAHG"
BASE COUNT          693 a         1357 c         1345 g          532 t
ORIGIN      
        1 agtcctcgcc gaacagcgtg atgcggcgct ggcgcagctt gtcctgcacg cgatgccggg
       61 cacgctccgc gaagtctggc agcagcccgc cgctcccgct caccaggctg atgcggctgt
      121 cgcggcccag cgcacgctcc agcgcaaagg ccagttcgac ggccagcacg tcgccgccga
      181 tcacgacgat gcacagcggc cgcgcctcgg ccagcgcgcg cagccgcggc cacagctggg
      241 agaaatgctg cagcggatgc acgaagaggc ccagctcgcg cgcgcccggc aaagcctcgg
      301 gggctatctc aggcgcaatg tccaggctca gcgcgtcata gccgattgcg ctgccgctgc
      361 tcagctgcag gctgcgcgcg gcgaggtcca gcgattgcac cgaggcctcg atgaaatccg
      421 ccccggccgc acgcgccagc gcagccaggg gcaggcggca gcgctcctcg ctcgcccgcc
      481 cggccaccca gcgcggcagc atcgcggcgc acaagggctc agcgctcggc gcgacgagac
      541 tgacgcggcc cacggccagg cgctcctggc ccaggctctt gaggacgtgc agatgggccg
      601 ggccatcgcc cagcagcagc aggtgtttca tcgttcgcgt tcagtgcatc gtcattcgag
      661 cgccagctcg gccacatagg ccaggctgcg ctgctgggta ttgtccgcgt ctgcgcccac
      721 ggccacgccg accagcgcag gcacgacctg gctctcatcg ccgaagaggc gcaggaaatc
      781 cgccgcaacg tcgcgccgct cggccaccca ttgatgcagg tgcgccgccc cgctctccag
      841 cacgatatag cgcacgcgct tggtgaaggc attgggcagc tcggtgccgg cgctcaaatg
      901 cgcatcccag acatagcaga cgctggccga gggcatgggc tcgctgctgc gcgcacgcat
      961 caggcgcagc agctggcgct cgacgaaagg cacccggtcc agcggcagct cgaacatcgc
     1021 gcacagcttg agcgtggtgt cgtcgcccga gcgcttgcgc aggtcggacg ccatgttgag
     1081 ctcctcgacg cgccattgcc agctcagcag atgcggcggt gcggcgccgg ccgtgaaatg
     1141 caggtcctgc gccagattgc cgtaggactt gtcggcctcg acgcgcagcg catgcttgcc
     1201 ttcgaagtcg ccgacggaaa agcgcgtcac gggcttggtc tggtgcggca gcagcgacca
     1261 gcgccagggc gggaccggct cgccacccac catcgcgaac ggcgccaggg cattgccggc
     1321 cgccgccgcg ccttgccagc ccagcgcgca caaaacgccc agcactgcgt ggcgccagcc
     1381 cggcacgatc cgcgctgcac tcatcgatcg cccccacacc gccgaatacc tgccgtcatt
     1441 gctgcggcag cattgcgatg agatccgtca cgccgggatc gacgccggct tgcatcagca
     1501 gcgtcgtcgc ctggccgcgg tggtgcgtct ggtggttgaa gaaatggctg aatgcatacc
     1561 aggcttcatg ctcgcgcggc acgcccttgc tggtggcgta gcgcatgagg cgcgtcacgt
     1621 catccggcgt cagttcggca gtccaggcct cgatcgcagc atccatctcg gcgcgatgcg
     1681 cgcgcatggc ctcgaagtcg tcgaacaaga cctgatcgag gccggtaaag gacggcatcg
     1741 cgagcacggc gggcgtcagc gccgcgaagc gcttatctag ggccgcaaaa cggttcagcc
     1801 agaccttgtc ggccagcacc agatgagcga gcgtgttgtg cagcgagccg aagaaggcgc
     1861 cgcgatcgcg cttgcgttcc gcgtcgctca ggccggatgc cgccgcatag aggcgctcgt
     1921 tcatccagcg gttgtaacgc gccgtgagac ggtagtgttc gagcatggcg caagtgtaga
     1981 caagcgccct gctcggcctg tatcaccgtg tgctgagccg gcgcaagacc agggtatcga
     2041 gcgccagctt gcccggcccg aagaccgtga gcagggccag cagcgagccc cagtaaaagt
     2101 gcgagttcac cggagcgggg cattcgaact cgaacagctg cggataggag acgacggcaa
     2161 ccaggttgac cacgaacagg gccgccgcgg cgaagcggcc ggccaggccc agcgcgatca
     2221 gcaccgggaa cacgagttcg ccgaaagcgc cgctgtaggc ggccacgtcc gggggcagca
     2281 gcggcacgtg gtactcgtag tggaacagcg cgagcgtgcc atgccaattg cccagcttgg
     2341 ccaggccgga attgaagaac acctgggcca cataaaggcg catcgccagc agcgccacgg
     2401 gctgcaggcg atcgatccag accgtcacgc ccagggcctg ccgcaagagg cgggacatca
     2461 tgtgtttgct ccttcgggcg acgcgaagcc gcagatcagg ttagcccgga ctgctgcgcc
     2521 gagcgccgcc gcgagctcga agcccgcatc ggcatccgag gccagcctgg cggcccgctc
     2581 caaggacgcg ccgccgagca gcccggcaag aaacattgcc atgccaggcc ccacggccga
     2641 gaccagcaca tcgaggcctc ggcgcgtcac cagggccgtc tcggcgcggt cccaatcgac
     2701 gctgaattcg ccgtcgtagc cgtcctgatg gatggtccac aggcgcacga tcgggtgtgc
     2761 cgagcggatc agcgcggtgc ccggcgccaa ctggaagcgc aggcccgcct gctgcgcggc
     2821 cggcacccgg gccagggcct gcagatcgaa caaaggcgca tcggccgcgg cgtaggcgcg
     2881 atgcgccgcc cattcgagcc gcgccaggtc cggcagatag gcaaactccg caaccggcgg
     2941 aaactgcgcc agaaactgcg cgaagcctgc gccgtagtcg tacagatcgc cgctggtgga
     3001 cgggcactcg cgcaagtggg cgcgtgccaa gccgccgaaa aactcctcgc cgacgatctg
     3061 gacgatgacc ggataggcgg ccgccagcgc cttggcgacg gcaccgacgg cattgccgcg
     3121 gtagatcgcc agccgccgct gcacgagcgc ctgttcgccc ttgaacaggt gcagcgcggg
     3181 ttccgtttgc gccgcatcgc gcagcgcggc ggcgaaggcg cgctggccct gcagcagcgc
     3241 agtgggccgc tcagccatgg gccggctcca gcagggctga ctgcagctcg cgcgctaggc
     3301 gcgcttcctc cagcaggacc gacagggccg gcaagtcggt gtcccactcg atcagcgtag
     3361 gcttggggcc gaagcgcgcg caggcctgct cgtacaagcg ccagacgtcc ggcgacacgc
     3421 gcgagccgtg gtggtcgatc aggccgtcat cggtgcgcaa atgtccagcc aggtgaatct
     3481 ggccgatggc ggagcccggc aactcggcca tcagcgcaag cgcatcgccg ccgaagttga
     3541 cggcgttgac gtagagattg ttgacgtcga acaggacgcc gcagccagtg cggcgcgaca
     3601 gctcggcgag gaagctcgtc tcgctcattt cggcgcctcg gtattcgaca taggccgaga
     3661 ggttctcgac caggatggtg cggcgcagcg tgtcctgcac gcgggcgacg cgatcggcca
     3721 gcaagcgcag cgcttcttcg gtatagggca agggcaggag gtcgttgaag tggaccccgt
     3781 cgagcgcgcc ccagcacaaa tgctcggaca ccagcgcggg ctcgacccgg tcgaccaatt
     3841 gcttgagctt ggccagatgg cgctgcgcca aggcgtcggc cgaacccagc gacaagccga
     3901 cgccgtgcag gctgatcggg taggcgt
//
LOCUS       DASUCY010000604         2237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_688239, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000604 DASUCY010000000
VERSION     DASUCY010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688239"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..1184)
                     /gene="gap"
                     /locus_tag="VFV25_11950"
     CDS             complement(186..1184)
                     /gene="gap"
                     /locus_tag="VFV25_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEY1394081.1"
                     /translation="MTVKIGINGFGRIGRMVFRAALANFQDIEVVAINDLLEPDYLAY
                     MLQYDSVHGRFKGEIAVDGSTLIVGGKKIRLTAIKDPAELNWGAVGADIVVESTGLFL
                     TKETAEKHLKAGAKKVILSAPSKDDTPMFVYGVNHESYKGEAIISNASCTTNCLAPLA
                     KVLNDKWGIKRGLMTTVHATTATQKTVDGPSNKDWRGGRGILENIIPSSTGAAKAVGV
                     VIPELNKKLTGMSFRVPTSDVSVVDLTVELVKEASYKDICAEMKAQSQGALKGVLGYT
                     EDKVVATDFRGDPRTSIFDAEAGIALDGTFVKLVSWYDNEWGYSNKVLEMARVIAK"
     gene            complement(1259..>2237)
                     /locus_tag="VFV25_11955"
     CDS             complement(1259..>2237)
                     /locus_tag="VFV25_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEY1394082.1"
                     /translation="AHTKAETVASRKASQIALEAFTAALPELLGGSADLTGSNLTNTK
                     STPALRFDAATGASNGGRHINYGVREFGMAAIMNGVALHGGYIPYGGTFLTFSDYSRN
                     AIRMAALMKERVIHVFTHDSIGLGEDGPTHQSIEHAASLRLIPGLDVWRPGDTAETAV
                     AWACAIENRQRPSALLLSRQNLPYAPKSGLDDIAKGAYVLAEPSEVGLAKKAQAVIIA
                     TGSELQLAMHAQQMLALQGIAVRVVSMPSTTVFDRQDADYKTSVLPQGLPRVAVEAGV
                     TGFWWKYGVAAVVGIDSYGESAPANVLFKHFGFTAENVVDTVKVVLGRH"
BASE COUNT          336 a          736 c          752 g          413 t
ORIGIN      
        1 ctcctgtggc tgctgggcgc tcgtcgctgc agtggcggcg gcgagccatt caaggcagtt
       61 tatggctgtg cgagcgctgc gggcgcgcct tacgttgctt tacaagatgt agcgtcagcg
      121 cggcgcaagc cccaccaaga aaaaggcccc gcagtgcggg gccctgagcg gaaaccgggc
      181 agcgtttact tggcgatgac gcgcgccatc tccagcacct tgttcgaata gccccattcg
      241 ttgtcgtacc aggacacgag cttcacgaag gtgccgtcca gcgcgatgcc ggcctcggcg
      301 tcgaagatcg aggtgcgggg gtcgccgcgg aaatcggtgg cgaccacctt gtcctcggtg
      361 tagcccagca cgcccttgag cgcgccttgg ctctgcgcct tcatctcggc acagatgtcc
      421 ttgtagctcg cttccttgac cagctccacc gtcaggtcga ccacggacac gtccgaggtc
      481 ggcacgcgga agctcatgcc ggtgagcttc ttgttcagct cggggatcac cacgcccacc
      541 gccttggcgg cgccggtgct gctggggatg atgttctcga ggatgccgcg gccgccgcgc
      601 cagtccttgt tgctggggcc gtccacggtc ttctgcgtcg ccgtggtggc gtgaacggtg
      661 gtcatcaggc cgcgcttgat gccccacttg tcgttcagca ccttggccag cggggccagg
      721 cagttggtcg tgcacgaggc attggagatg atggcctcgc ccttgtagct ttcgtgattg
      781 acaccgtaga cgaacatcgg cgtgtcgtcc ttcgaggggg ccgacaggat gaccttcttg
      841 gcgccggcct tcaggtgctt ctcggccgtt tccttggtga ggaagaggcc agtcgattcg
      901 accacgatgt cggcgccgac ggcgccccag ttcagctcgg ccgggtcctt gatcgcggtg
      961 aggcggatct tcttgccgcc cacgatcagc gtgctgccgt cgaccgcgat ctcgcccttg
     1021 aagcggccgt gcaccgagtc gtactgcagc atgtaggcca ggtagtcggg ctccagcagg
     1081 tcgttgatgg cgacgacttc gatgtcctga aagttcgcca gcgccgcgcg gaacaccatg
     1141 cgcccgatgc gcccgaagcc gttgatgccg atcttcaccg tcatgttcac tctcctagaa
     1201 aaagaaaacc tgattcgcaa agcaagcggg agcccggcag gctcccgtca cgcatgtcct
     1261 agtgccggcc cagcaccacc ttcaccgtgt ccacgacatt ctcggccgtg aacccgaagt
     1321 gcttgaacag cacattggcc ggcgccgact cgccgtagct gtcgatgccg accacggcgg
     1381 caacgccgta cttccaccag aagccggtca cgccggcctc caccgcgacg cgcggcaggc
     1441 cctgcggcag cacgctcgtc ttgtagtcgg cgtcctgccg gtcgaacacc gtggtcgagg
     1501 gcatggacac cacgcgcacc gcgatgccct gcagcgccag catctgctgc gcatgcatcg
     1561 ccagctgcag ctcggagccg gtcgcgatga tcaccgcttg cgccttcttc gccaggccca
     1621 cctcgctcgg ctcggccagc acataggcgc ccttggcgat gtcgtccagc ccgctcttgg
     1681 gcgcataggg caggttctgg cgggagagga gcagcgcgct cgggcgctgc ctgttctcga
     1741 tcgcgcaggc ccaggccacc gcggtctcgg cggtatcgcc ggggcgccag acgtcgaggc
     1801 cgggaatgag ccgcagcgat gcggcatgct cgatggactg gtgggtcggc ccgtcttcgc
     1861 ccaggccgat ggagtcgtgc gtgaagacat ggatcacgcg ctccttcatc agcgcggcca
     1921 tgcggatggc gttgcggctg tagtcgctga aggtcaggaa ggtgccgccg taggggatgt
     1981 agccgccgtg cagcgccacg ccgttcatga tggcggccat gccgaactcg cgcacgccgt
     2041 agttgatgtg gcgcccgccg ttggaggcgc cggtggccgc atcgaagcgc agcgcaggcg
     2101 tgctcttggt gttggtgagg ttggagccgg tgaggtcggc cgagccgccc agcagctcgg
     2161 gcagcgcggc ggtgaaggcc tccagcgcga tctggctggc cttgcggctg gccaccgtct
     2221 cggccttggt gtgcgcg
//
LOCUS       DASUCY010000605         8223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1218949, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000605 DASUCY010000000
VERSION     DASUCY010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 8223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8223
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218949"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..961)
                     /gene="hflC"
                     /locus_tag="VFV25_11960"
     CDS             complement(68..961)
                     /gene="hflC"
                     /locus_tag="VFV25_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HEY1394083.1"
                     /translation="MNKIGMWVIGALLALLILSSTLFVVDQRQFAVVYALGEIKEVVS
                     EPGLKFKLPPPFQNVVFLDRRIQSLDSPETRPMFTAEKKSLVVDWLVKWRIVDPKQFI
                     RNNGVDVRNVENRLSPIVQAAVNEEVTKRDVRAVLSTEREKVMQGVLQRLSDDARSFG
                     IEIVDVRIKRVDFSANITDAVYRRMQSERQRVANELRSQGAAEKEKIQADADRQREII
                     VAEAYRDAQKIKGEGDAKASSLYADAFGRDPAFAKFYRSLEAYRASFRSKSDVMVIDP
                     SSEFFKAMRGSDSGAADGKRK"
     gene            complement(982..2229)
                     /gene="hflK"
                     /locus_tag="VFV25_11965"
     CDS             complement(982..2229)
                     /gene="hflK"
                     /locus_tag="VFV25_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HEY1394084.1"
                     /translation="MASRAAWTLARILPGPARPQRLESNGRNEGPPDLDELWRDLNRK
                     LSGLFGGKGGGADSGGGGSNFQPNMKSTGIGAGLIGGVVALIWLGSGFFIVQEGQQAV
                     VMSFGKFSHIADAGFQWRWPYPLQSHEIVNVTQLQTREVGRSQIVQATGLRDSSMLTQ
                     DENIVDIRFTVQYSLKDAKDYLFENRNPDEAVTLAAESAVREIVGKSNMNSVLYEQRD
                     AIAAELVKSIQAQLDRLKAGIVIRNVNVQGVQPPEQVQAAFDDAVKAGADRERFKNEG
                     QAYANDVIPKAQGTASRLHEDAQGYRASVVATAEGDAQRFRSVLAEYQRAPAVTRDRM
                     YIDAMQQVYANVSKVMVESRSGSNLLYLPLDKLIQQAAAASVTAPAASAPTPAGTQPD
                     TGSLVSGDSRSRDGLRSRDRETR"
     gene            complement(2313..3575)
                     /gene="hflX"
                     /locus_tag="VFV25_11970"
     CDS             complement(2313..3575)
                     /gene="hflX"
                     /locus_tag="VFV25_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEY1394085.1"
                     /translation="MTELHSSPAGASGGVEARAILVGVDLGPAAAFDPTLDELALLAE
                     SAGDTPVARLTAKRKAPDAALFVGSGKADEIKLLVDAHQAHCVIFDQALSPAQQRNLE
                     RHIGVEVLDRTMLILEIFAARAQSHEGKLQVELARLEYLSTRLVRRWSHLERQRGGVG
                     HRGGPGETQIELDRRMIAQRIKSLKERLARVKRQRGTQRRSRERNGTFRVSLVGYTNA
                     GKSTLFNALVKARAYAADQLFATLDTTTRQLYLEEAQRSVSLSDTVGFIRDLPHTLVD
                     AFEATLLEASDADLLLHVVDAASPVLLEQMEQVQKVLHEIGAGDLPQLLVFNKLDCLE
                     ADRRPREMVDAIEIDAGRRVPRVFISARDGEGLDRLRRLIAAAALGRADLKRGDWASA
                     RAEEPASSDAMIAAVPAWPATGTFPSNT"
     gene            complement(3646..3891)
                     /gene="hfq"
                     /locus_tag="VFV25_11975"
     CDS             complement(3646..3891)
                     /gene="hfq"
                     /locus_tag="VFV25_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEY1394086.1"
                     /translation="MSNKGQLLQDPFLNLLRKEHVPVSIYLVNGIKLQGHIESFDQYV
                     VLLRNTVTQMVYKHAISTVVPGRAVNFHAAPESPEAN"
     gene            complement(3963..5303)
                     /gene="der"
                     /locus_tag="VFV25_11980"
     CDS             complement(3963..5303)
                     /gene="der"
                     /locus_tag="VFV25_11980"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEY1394087.1"
                     /translation="MKPVLALVGRPNVGKSTLFNRLTKSRDAIVADFAGLTRDRHYGD
                     GRLGEREFIVIDTGGFEPTAESGIYKEMAKQTRQAVAEADAVVFVVDVRDGLSAQDFD
                     IARYLRSVNKKVLLAANKAEGMSEGLQLAEFHELGIGMPHPVSAAHGQGIRSLVEEAL
                     DGFDYGEQEEPALDDPERPIRLAVAGRPNVGKSTLINAWLGEERLVAFDLPGTTRDAI
                     SVPFERNGQRFELIDTAGLRRKGKVFEAIEKFSVVKTLQAIADANVVLLLLDATQGVT
                     DQDAHIAGYILDSGRAVVLALNKWDAVDSYQREMLQRSIEQRLAFLKFAPVLHISAIK
                     RQGLGPVWKAIAEAWASATKKMPTPVLTRLLYEAVEFQQPKRAGAFRPKLRYAHQGGM
                     NPPLIVVHGNSLEHVTDAYKRYLEGRFREHFKLSGTPLRIELRSSRNPFREKDS"
     gene            complement(5335..6468)
                     /gene="bamB"
                     /locus_tag="VFV25_11985"
     CDS             complement(5335..6468)
                     /gene="bamB"
                     /locus_tag="VFV25_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HEY1394088.1"
                     /translation="MSWAFLRRRQRSLAVVALGLALAACSSTTKPKPTPLEAFEPKLQ
                     GHVVWSQHLGGRILPSQPSTKGEVFTVASSNGTVAALDVESGREVWRADAGDKLSAGA
                     GSDGRYASVVTIDNELVTFDQGKPIWRLRLGSKVVTPPLVAGERVFVQGVDRAVLAFD
                     AQDGRKLWVMQRQGDPLALSQPGVLTSYRDTLLAGQGAKLVGIDPLLGTQRFELGLAS
                     PRGTNEVERLADLVGPAARADDTVCARAFQAAVACVNAERASVMWSKTVSGYQGVSAD
                     TQFVFGADANDRITAWQRASGEVAWTSERLLHRGLSAPLALGRAVVFGDAEGYVHFLS
                     REDGQTLMRVPTDGSAIVGAPLRSGATVAVLTANGAVYAIRPE"
     gene            complement(6465..7142)
                     /locus_tag="VFV25_11990"
     CDS             complement(6465..7142)
                     /locus_tag="VFV25_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEY1394089.1"
                     /translation="MATHLDLEEQEQLDQLKHFWNQYGNLITWVLILALAGFAAFNGW
                     NWYKREQGAKAAALYEELDKAVQGGDVDKATLVFSDMKQRYAGTAYAEQAGLLAAKLQ
                     FEKNKPDAAQASLEWVAGNAMENEYRIIARLRLAGALLDQKKYDDALKQLPSDAPKEF
                     EALVADRRGDILMAQGKKEEAKAAYQQAWKSMDAGIDYRRLIEAKLTALGAAPAAPDA
                     AASGAAK"
     gene            complement(7171..>8223)
                     /gene="hisS"
                     /locus_tag="VFV25_11995"
     CDS             complement(7171..>8223)
                     /gene="hisS"
                     /locus_tag="VFV25_11995"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEY1394090.1"
                     /translation="LRPEATAGIVRAMVEHNALYNGPLRVWTMGAVFRHERPQKGRYR
                     QFHQIDVEALGYAGPDVDAEQILMCRALWRELGLVEGEDVRLEINSLGQPDERRKHRE
                     ALVAHFQAHAELLDEDARRRLHSNPLRILDSKNPAMQAMIEATPKLMDFLGEASLAHL
                     NAVRAILDAAGLAYRVNPRLVRGMDYYNLTVFEWVTDKLGSQGTVCGGGRYDGLFEQL
                     GGKPTPAIGWGLGVERLLLLLEELGKPLPDDAPHAYAVVPTAAAAAPAMKLIEVLRAA
                     GVAVLMHAAGKDGPGSMKSQFKRADASGARYALVFGEAELAQGMVALKALRNEGEQVL
                     RPLDRPTDWAVELLRA"
BASE COUNT         1200 a         2785 c         2829 g         1409 t
ORIGIN      
        1 gcagaccctc gatcaccagc accagcgcga aggcgctgaa caaggtttgc cacatggcgc
       61 gccgcgccta tttccgcttg ccgtctgcag cgccgctgtc gctgccgcgc atggccttga
      121 agaattcgct gctcggatcg atcaccatca cgtcggactt gctgcggaag ctcgcgcgat
      181 aggcctcaag gctgcggtag aacttcgcga aggccgggtc gcggccgaag gcgtcggcat
      241 acaggctgga agccttggca tcgccctcgc ccttgatctt ctgcgcatcg cgataggcct
      301 cggcgacgat gatttcgcgc tggcggtcag catcggcctg gatcttctcc ttctcggcgg
      361 cgccctgcga acgcagttcg ttggccacgc gctggcgttc ggactgcatg cgccgataga
      421 cggcatcggt gatgttggcc gagaagtcca cgcgcttgat gcgcacgtcg acgatctcga
      481 tgccgaagga gcgcgcgtcg tcgctcaggc gctgcaggac gccctgcatc accttctcgc
      541 gctcggtgga gagcacggcg cgcacgtcgc gcttggtcac ttcctcgttc accgccgcct
      601 gcacgatggg gctgagccgg ttctcgacgt tgcgcacgtc cacgccgttg ttgcggatga
      661 actgcttggg gtcgacgatg cgccatttga ccagccagtc caccaccagg ctcttcttct
      721 cggccgtgaa catgggccgc gtctcggggc tgtccaggct ttgtatgcgc cggtcgagga
      781 agaccacgtt ctggaagggc ggcggcagct tgaacttcag cccgggctcg gagacgacct
      841 ccttgatctc gcccagcgca tacaccaccg cgaactgccg ctgatccacc acgaacaggg
      901 tggaggaaag aatcaacagc gccagcagcg caccgatgac ccacatgccg atcttgttca
      961 tatcgttctc gtcgtgcgca ttcagcgggt ttcgcggtcg cggctgcgca gcccgtcgcg
     1021 cgagcgggag tcgcccgaaa cgagggaacc ggtatcaggc tgcgtgcccg ccggtgtcgg
     1081 tgccgacgcg gcgggcgccg tcacgctggc cgcggccgcc tgctgaatca gcttgtccag
     1141 cggcaggtac agcaggttcg aaccgctgcg cgactccacc atcaccttgc tgacgttggc
     1201 atagacctgc tgcatggcgt cgatgtacat gcggtcgcgc gtcacggcgg gcgcgcgctg
     1261 gtactcggcg agcacgctgc ggaagcgctg cgcatcgccc tcggcggtgg cgaccacgga
     1321 ggcgcggtag ccctgggcgt cctcgtgtag ccgcgaggcc gtgccctgcg ccttggggat
     1381 cacgtcgttg gcataggcct ggccttcgtt cttgaagcgc tcgcggtcgg cgccggcctt
     1441 caccgcgtcg tcgaaggctg cctgcacctg ctcgggcggc tgtacgccct gcacgttgac
     1501 attgcggatc acgatgccgg ccttgaggcg gtcaagctgg gcctggatcg acttgaccag
     1561 ttcggcggcg atggcgtcgc gctgctcgta gagcaccgag ttcatgttgc tcttgccgac
     1621 gatctcgcgc acggccgact cggccgccag cgtgacggcc tcgtcggggt tgcggttctc
     1681 gaacaggtag tccttggcgt ccttgagcga gtactgcacc gtgaagcgga tgtcgacgat
     1741 gttctcgtcc tgggtcagca tcgacgagtc gcgcagcccg gtggcctgca cgatctggct
     1801 gcgccccact tcgcgcgtct gcagctgggt cacgttgacg atctcgtgcg actgcagcgg
     1861 atagggccag cgccactgga agccggcgtc cgcgatatgg ctgaacttgc cgaaggacat
     1921 cacgaccgcc tgctgcccct cctgcacgat gaagaagccg ctgcccagcc agatcagcgc
     1981 cacgacgccg ccaatcaggc cggcgcctat gccggtgctc ttcatgttgg gctggaaatt
     2041 ggagcctccg ccgccgctat cggccccgcc gcccttgccg ccgaacaggc cgctgagctt
     2101 gcggttgagg tcgcgccaca gctcgtccag atcgggcgga ccttcattgc ggccattgct
     2161 ttcgaggcgc tggggccgcg caggtccggg caggatgcgg gccagcgtcc aggcggcacg
     2221 gctcgccatc cccgctccca aggcggcgat cgccgccatc aaggccggcc agcggtgcgc
     2281 tggcaaggcc gcgtcatttg cacttcttct gttcatgtgt tgctaggaaa agtccctgtg
     2341 gcaggccatg ccggcacggc cgcgatcatc gcatcacttg atgccggttc ctcggctcga
     2401 gctgaggccc aatcgccgcg cttcaagtcc gctcgcccca gcgccgcagc ggcgatcagc
     2461 cggcgcagcc gatccaggcc ctcgccgtcc ctggcgctga tgaagacccg gggcacgcgc
     2521 cggccggcgt cgatctcgat cgcatcgacc atctcgcgcg gcctgcgatc ggcctccagg
     2581 cagtccagct tgttgaagac gagcagctga ggcaggtcgc cggcgccgat ctcgtgcagc
     2641 accttctgca cctgctccat ctgctccagc agcaccgggc tggcggcatc gaccacatgc
     2701 agcagcaggt cggcatcgga cgcctccagc agcgtggcct cgaaggcgtc gaccagcgtg
     2761 tgcggcaggt cgcggatgaa acccaccgtg tcggacagcg aaaccgagcg ctgagcctct
     2821 tcgaggtaga gctggcgcgt cgtcgtgtcc agcgtcgcga acagctggtc ggccgcgtag
     2881 gcgcgcgcct tcaccagggc attgaacagc gtcgacttgc cggcattggt gtagccgacc
     2941 agcgagacgc ggaaggtgcc gttgcgctcg cgcgagcgcc gctgcgtgcc gcgctggcgc
     3001 ttgacgcgcg cgagcctttc cttgagcgac ttgatgcgct gcgcgatcat gcgccggtcc
     3061 agctcgatct gggtctcgcc cgggccgccg cgatggccga cgccgccgcg ctggcgctcc
     3121 aggtgcgacc agcgccgcac caggcgggtg gagaggtatt cgagccgcgc cagttcgacc
     3181 tgcagcttgc cctcgtggct ctgggcgcgg gccgcgaaga tttccaggat cagcatggtg
     3241 cggtcgagca cttcgacgcc gatgtgccgc tcgaggttgc gctgctgggc cggcgagagc
     3301 gcctggtcga agatgacgca atgcgcctgg tgcgcgtcga ccagcagctt gatctcgtcg
     3361 gccttgcccg agccgacgaa gagggccgcg tcgggcgcct tgcgcttggc tgtgagcctg
     3421 gccaccggcg tgtcgccggc cgactcggcc agcagcgcca gctcgtccag cgtcgggtcg
     3481 aaggcggccg ccggaccgag atcgacgccg accaggatcg ccctggcctc gacgccgccg
     3541 ctggcgccgg cggggctgga atggagttcg gtcaaggctg aatccggcgc tgaagccgca
     3601 gaccgccgcg cggcgcagag gagggagctg cggcgcggcg gacgctcaat tcgcctcggg
     3661 gctctcgggg gccgcgtgga agttcaccgc gcggccgggc acgacggtgg agatcgcgtg
     3721 cttgtagacc atctgggtca cggtgttgcg cagcaggaca acgtactggt cgaaggattc
     3781 gatgtgcccc tggagcttga tgccgttgac gaggtagatg gagaccggca catgctcctt
     3841 gcgcagcagg ttcaggaacg ggtcttgtag gagttgccct ttgttactca cgatatgctc
     3901 cgtgttgaaa agttgttgga gattgggacc ttaacacagc ctcatgctgc acagcgcaaa
     3961 agtcaggaat ccttttccct gaaggggttg cgcgaagagc gcagctcgat gcgcagcggg
     4021 gtgcccgaga gcttgaaatg ctcgcggaag cgcccttcca ggtagcgctt gtaggcatcg
     4081 gtcacgtgct cgagcgaatt gccgtggacc acgatcagcg gcgggttcat gccgccctgg
     4141 tgcgcgtagc gcagcttggg acggaaagca cccgcccgct tgggctgctg gaactccacc
     4201 gcctcgtaca agagccgcgt gagcaccggc gtgggcatct tcttggtggc cgaggcccag
     4261 gcctcggcga tcgccttcca caccggcccc aggccctggc gcttgatggc cgagatgtgc
     4321 agcaccggcg cgaacttcag gaaggccagc cgctgctcga tcgagcgctg cagcatctcg
     4381 cgctggtagc tgtccaccgc atcccatttg ttcagcgcca gcacgacggc gcggccggaa
     4441 tcgaggatgt agccggcgat gtgcgcgtcc tggtcggtca cgccctgagt cgcgtcgagc
     4501 agcagcagca cgacgttggc atcggcgatg gcctgcaggg tcttgacgac cgagaacttc
     4561 tcgatcgcct cgaagacctt gcccttgcgg cgcagccccg cggtgtcgat cagctcgaag
     4621 cgctggccgt tgcgctcgaa gggcacgctg atggcgtcgc gtgtggtgcc gggcaggtcg
     4681 aaggccacca ggcgctcctc gcccagccag gcattgatca gcgtggactt gccgacattg
     4741 gggcggcccg ccaccgcgag gcgaatcggc cgctcgggat cgtcgagcgc gggttcctct
     4801 tgctcgccgt agtcgaagcc gtcgagcgcc tcctcgacca ggctgcggat gccctgcccg
     4861 tgcgcggccg acaccggatg cggcatgccg atgcccagct cgtggaactc ggccagctgc
     4921 aggccctcgg acatgccctc ggccttgttg gccgccagca gcaccttctt gttgacgctg
     4981 cgcaggtagc gcgcgatgtc gaagtcctgc gccgacaggc cgtcgcgcac gtcgaccacg
     5041 aagaccactg cgtcggcctc ggccacggcc tggcgcgtct gcttggccat ctccttgtag
     5101 atgccgctct ctgccgtcgg ctcgaagccg ccggtgtcga tgacgatgaa ctcgcgctcg
     5161 cccaggcggc cgtcgccgta gtggcggtcg cgcgtgagcc cggcgaaatc ggccacgatg
     5221 gcgtcgcggc tcttggtgag ccggttgaac agcgtggatt tgccgacgtt ggggcggccg
     5281 accagcgcca gcacgggttt catgatggga ccttggcgta ctgcggtgct ggcttcactc
     5341 ggggcggatg gcgtagaccg cgccgttggc ggtcagcacc gcgaccgtgg cgcccgagcg
     5401 caacggcgcg ccgacgatgg cgctgccgtc ggtcggcacg cgcatcaggg tctgcccgtc
     5461 ctcgcgcgac aggaaatgca catagccctc tgcgtcgccg aagaccacgg cgcggcccag
     5521 cgccagcggt gcgctcaggc cccggtgcag caggcgctcg gaagtccagg ccacctcgcc
     5581 gctggcccgc tgccaggcgg tgatgcggtc gttggcatcg gcgccgaaga cgaactgggt
     5641 gtcggcgctg acgccctgat agccgctcac ggtcttggac cacatcacgc tggcccgctc
     5701 ggcgttgacg caggccacgg cggcctggaa ggcgcgggcg cagaccgtat cgtcggcgcg
     5761 cgccgcgggg cccaccaggt cggccaggcg ctccacctcg ttggtgccgc gcggcgaagc
     5821 gaggccgagc tcgaagcgtt gcgtgcccag cagcggatcg atgccgacca gcttggcgcc
     5881 ctgcccggcc agcagcgtat ccctgtagga cgtgagcacg ccgggctggg acaaggccag
     5941 cggatcgccc tggcgctgca tgacccagag cttgcggccg tcctgcgcgt cgaaggcgag
     6001 cacggcgcga tcgacgccct gcacgaagac gcgctctccg gccaccagcg gcggcgtcac
     6061 gaccttggag cccaggcgca ggcgccagat cggcttgccc tggtcgaagg tgacgagttc
     6121 gttgtcgatg gtgacgacgc tggcatagcg gccgtcactg cctgcgccgg cgctcagctt
     6181 gtcgcccgcg tcggcacgcc agacttcgcg gccggactcg acgtccagcg cggcgaccgt
     6241 gccgttggag ctagccaccg tgaagacctc gcccttggtg gagggctgcg aaggcaggat
     6301 gcgcccgccc agatgctggc tccagaccac atggccctgg agcttgggct cgaaggcttc
     6361 cagcggcgtc ggcttgggct tggtggtgct ggagcaggcg gcgagcgcca ggcccagcgc
     6421 gaccacggcc aggctgcgct gccggcgccg caggaaggcc cagctcattt ggcggctccg
     6481 gaggcggccg cgtcgggtgc cgccggcgcg gcgcccagcg cagtgagctt ggcctcgatc
     6541 aggcggcggt agtcaatgcc cgcgtccatg ctcttccagg cctgctggta ggcggccttg
     6601 gcttcttcct tcttgccctg ggccatcagg atgtcgccgc ggcggtcggc gaccagggcc
     6661 tcgaactcct tgggcgcatc gctgggcagc tgcttgagcg cgtcgtcgta cttcttctgg
     6721 tccagtagcg cgccggccag ccgcaggcgg gcaatgatgc ggtattcgtt ttccatcgca
     6781 ttgccggcca cccactcgag gctggcctgg gccgcgtcgg gcttgttctt ctcgaactgg
     6841 agcttggccg ccagcaggcc ggcctgctcg gcataggcgg tgccggcata gcgctgcttc
     6901 atgtcgctga agaccagcgt ggccttgtcg acgtccccgc cctgcacggc cttgtccagc
     6961 tcttcgtaca gcgcggcggc cttggccccc tgctcgcgct tgtaccagtt ccagccgttg
     7021 aaggccgcga agccggccag cgccaggatc agcacccagg tgatcagatt gccgtactgg
     7081 ttccagaaat gcttgagctg gtcgagctgt tcttgttctt cgaggtcgag atgcgtggcc
     7141 atgtgtcttc ggtatacgcg aaagcgtgga ttatgcgcgg agcagttcta cggcccagtc
     7201 ggtggggcgg tccagcgggc gcagcacctg ctcgccctca ttgcgcaggg ctttcagtgc
     7261 caccatgccc tgggccagct cggcctcgcc gaagaccagc gcgtagcgcg cgccgctcgc
     7321 gtcggcgcgc ttgaactgcg acttcatgct gcccggcccg tccttgcccg cggcgtgcat
     7381 cagcaccgcc acaccggcag cacgcagcac ttcgatcagc ttcatcgccg gcgcagcggc
     7441 agcagccgtt ggcacgacgg cataggcatg cggcgcgtca tcaggcagcg gcttgccgag
     7501 ttcctcgagc agcagcagca gccgctcgac gcccaggccc cagccgatcg ccggcgtggg
     7561 cttgccgccc agctgctcga acaggccgtc atagcgcccg ccgccgcaga ccgtgccctg
     7621 ggagccgagc ttgtcggtga cccactcgaa gacggtgagg ttgtagtagt ccatgccgcg
     7681 caccaggcgc ggattgacgc ggtaggcaag ccccgccgca tcgaggatgg cgcgcaccgc
     7741 attcaggtgc gcgagcgagg cctcgccgag gaaatccatc agcttgggcg tcgcctcgat
     7801 catggcctgc atcgccgggt ttttgctgtc gaggatgcgc agagggttgc tgtgcagacg
     7861 gcgcctggcg tcctcgtcga gcagctcggc atgggcctgg aaatgcgcga ccagcgcctc
     7921 gcggtgcttg cggcgctcgt cgggctggcc gaggctgttg atctccagtc gcacgtcctc
     7981 gccttcgacc aggcccagct cgcgccacag cgcgcggcac atcaggatct gctcggcgtc
     8041 cacgtcgggc ccggcatagc ccagggcctc gacgtcgatc tggtggaact ggcggtagcg
     8101 gcccttctgc ggccgctcgt ggcggaacac cgcgcccatg gtccagacgc gcaacgggcc
     8161 gttgtagagc gcgttgtgct cgaccatcgc gcgcacgatg ccggccgtgg cctcggggcg
     8221 cag
//
LOCUS       DASUCY010000606         5767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_913100, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000606 DASUCY010000000
VERSION     DASUCY010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 5767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5767
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913100"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="VFV25_12000"
     CDS             complement(<1..580)
                     /locus_tag="VFV25_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HEY1394091.1"
                     /translation="MRWSQILFSQGFGTRRVCTGLIDAGLVRCQGRVVQADDEEFEPE
                     EGLLFEVEGQPWAYHRSALVMLYKPAGYECSQKPRHHPSVLSLLPTPLRQRGVQPIGR
                     LDEDTTGLLLLTDDGALLHKLTSPKHHVPKVYEVGAKHPITPQQVDKLLAGVVLDDDP
                     RPVRADAAEQTGELALRLTLTQGKYHQVKRMLA"
     gene            complement(784..1911)
                     /locus_tag="VFV25_12005"
     CDS             complement(784..1911)
                     /locus_tag="VFV25_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY1394092.1"
                     /translation="MDFDFTEDQESLRDAVRRWVDKGFSFERRHALARAGGATRKVYG
                     ELAELGLTGLAVPEAHGGMGFGPVEAMVVMEELGRGLVNAPYAQGALIAPALLAAAPA
                     EIQAAWLPRVTDASALVVLAHQERKARYRLHHVTTKATKHGGAWALNGAKSIVPAGDE
                     ADAFIVPARISGAGEDRGGIGLFLVEKAAAQVRGYPTQDGARAAEVTFTNVAATLIAE
                     DGLAALEHAVDVGIAAVCAEGVGLMDKLVALTVEYMNTRKQFGVPIASFQALRHRIAD
                     VKMQLELGRSMSYFASLKLGEPAEARRRALAQAKLQLGNSMRYVGQQCIQLHGGIGVT
                     DEYIGSHYFKRLTVLEMTFGDTMHQLGEVSARMQDTAGVFA"
     gene            complement(1914..3095)
                     /locus_tag="VFV25_12010"
     CDS             complement(1914..3095)
                     /locus_tag="VFV25_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEY1394093.1"
                     /translation="MDLNFTAEELAFRDEIRAWVRANLPQDISHKVHNALRLSKDDMQ
                     RWAKILGKKGWHGWAWPKEFGGPGWNAVQRHLFEEECALAGAPRVVPFGPVMVAPVIM
                     AFGSPEQHRRFLPGIMSGEVWWSQGYSEPGSGSDLASLKTRAERKGDYYIVNGQKTWT
                     TLGQYGDWIFCLVRTSNEGKPQTGISFLLIDMKSPGITVRPIITMDGEHEVNEVFFDN
                     VEVPADNLVGEENKGWTYAKYLLAHERTNIADVNRAKRELERLKRIAKAEGVYEDSRL
                     RDQIALLEVDIVALEMMVLRVLSAEKGGKKALDIAGLLKIRGSEIQQRYTELMMLAAG
                     PYSLPFIHEAMEAGWQGDYVGAMHCAPLAATYFNVRKTTIYGGSNEVQRNIISQTVLG
                     A"
     gene            complement(3236..3877)
                     /locus_tag="VFV25_12015"
     CDS             complement(3236..3877)
                     /locus_tag="VFV25_12015"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HEY1394094.1"
                     /translation="MRALLCGALLAAAAPVFAQSVALSGSMGNKALLVIDGGAPRALA
                     PGESAQGVKLVSVSGGMAVIEVAGQRQSLVLGGAPVSVGGKGTSGGGGTKIVLSAGPG
                     GHFVAGGSINGRAVQFLVDTGATMVAMSFADAQRIGLKYEGGQRVGINTANGQAIGYR
                     VSLDSVRVNDVTVYNVDAVVQPADMPVVLLGNSFLGRFQMKRENDVLTLDKRY"
     gene            complement(3889..4377)
                     /locus_tag="VFV25_12020"
     CDS             complement(3889..4377)
                     /locus_tag="VFV25_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEY1394095.1"
                     /translation="MPSFDTVLEPNLVEVRNAIDQSNKEIGTRFDFKGSDARIEQKDK
                     ELTIYADSEFQLGQVMDILTAKLSKRNVDVRFLDTTPKIEKIGGDKVKQLVKVKAGIE
                     SELAKKIQKLIKESKMKLQAAIQGDTVRITGAKRDDLQAAMALIRKEVAEAPLSFNNF
                     RD"
     gene            4446..5462
                     /gene="murB"
                     /locus_tag="VFV25_12025"
     CDS             4446..5462
                     /gene="murB"
                     /locus_tag="VFV25_12025"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEY1394096.1"
                     /translation="MQIETGVSLKPYNTFGLNAVAEKLVRITSEADVRRVVDHPELGR
                     APKFILGGGSNIVLTRDVAACVLKIEVKGRRLLGQRDSALIAEVGAGENWHDTVAWTL
                     EQGWPGLENLALIPGTVGAAPVQNIGAYGVELKDRFESLDAVDLVTGRTVTLTREMCH
                     FGYRDSVFKQGLAGKSVITRVRLRLPQPWLAQLSYLELDRKRHETGIGNPSPQQVFDW
                     ICAIRRAKLPDPAEIGNVGSFFKNPVVTEEQCRDIIGRDPAIVHYPMPDGSVKLAAGW
                     LIDACGWKGKSIGGAGVYEKQALVLVNRGNASGGEVVTLARAIQESVYGRFGIRLEPE
                     PVVV"
     gene            complement(5473..>5767)
                     /locus_tag="VFV25_12030"
     CDS             complement(5473..>5767)
                     /locus_tag="VFV25_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY1394097.1"
                     /translation="GTSLGLTRVQTLRYVIGPQALRSAVPSLSNSLISLIKDTSLVSV
                     IAVTELMLATKEAISTTFQPFPLYIAAAGVYWVLSLSFEQVQKLLEKRLAFPH"
BASE COUNT          901 a         1910 c         1929 g         1027 t
ORIGIN      
        1 cggccagcat gcgcttgacc tggtggtact tgccctgggt cagcgtgagg cgcagagcga
       61 gttcaccggt ttgctcggcg gcgtccgcac gcacgggtcg gggatcgtcg tccagcacca
      121 cgccggcgag cagcttgtcg acctgctggg gtgtgatcgg atgcttggcg ccgacctcgt
      181 agaccttggg cacatggtgc ttgggcgagg tcagtttgtg cagcagcgcg ccgtcgtcgg
      241 taagcagcag caggcccgtc gtgtcctcat ccagcctgcc gatcggctgc acgccgcgct
      301 ggcgcagcgg cgtcggcagc aggctcagca cgctcgggtg atggcgcggc ttctgcgaac
      361 attcatagcc ggccggcttg tacagcatca cgagcgcgct gcgatgatag gcccagggct
      421 ggccctcgac ttcgaagagc aggccttcct cgggctcgaa ctcttcgtca tcggcctgga
      481 cgacgcgacc ctggcagcgc accaggccgg cgtcgatcag ccccgtgcag acgcgacggg
      541 tgccgaagcc ttggctgaac aggatctgtg accagcgcat tccttgcctc aaagaatacg
      601 gccgccatga tggcggccgc gcagcaagtt tagcctgggc actgcataac aatcgcccct
      661 cgcagcaccc cgcgtcgcat gaaccaagcc ttgccaagca gacgccgcgg aaccggcttt
      721 gccgggccgc tggcgtcgcc ccattgaggg ggagcgccga aggcgctacg ggggtgggtt
      781 atttcaggcg aagacgcccg ccgtgtcctg catgcgcgcc gacacctcgc cgagctggtg
      841 catggtgtcg ccgaaagtca tctcgagcac cgtcagccgc ttgaagtaat ggctgccgat
      901 gtattcgtcg gtcacgccga tgccgccgtg caactggatg cattgctggc cgacatagcg
      961 catcgaattg cccagctgca gcttggcctg ggccagggcc cggcggcgcg cctcggcggg
     1021 ttcgcccagc ttgaggctcg cgaagtagct catcgagcgg cccagctcca gctgcatctt
     1081 cacgtcggcg atgcggtggc gcagcgcctg gaagctcgcg atgggcacgc cgaattgctt
     1141 gcgcgtgttc atgtattcga cggtcagcgc gaccagcttg tccatcaggc cgacgccctc
     1201 ggcgcagact gccgcgatgc cgacgtcgac cgcatgctcc agcgctgcga ggccgtcctc
     1261 ggcgatcagc gtcgccgcta cattggtgaa cgtcacttcg gcggcgcgcg cgccgtcttg
     1321 cgtgggatag ccgcgcacct gcgcagccgc cttctcgacc aggaagaggc cgatgccgcc
     1381 gcgatcctcg ccggcgcccg agatgcgcgc cggcacgatg aaggcatcgg cctcgtcgcc
     1441 ggcgggcacg atgctcttcg cgccattgag ggcccaggcg ccgccatgct tcgtggcctt
     1501 ggtcgtcaca tggtggagcc ggtagcgagc cttgcgctcc tgatgcgcaa ggacgaccag
     1561 cgccgaggcg tcggtgaccc tgggcagcca ggcggcctgg atctcggccg gcgcggcggc
     1621 gagcagtgcc ggggcgatca aggcgccttg cgcatagggt gcattgacga ggcctcggcc
     1681 gagttcctcc atcaccacca ttgcctcgac cggtccgaaa cccatgccgc catgtgcctc
     1741 gggcaccgcg aggccggtca ggcccagctc ggccagctcg ccatagacct tgcgggtggc
     1801 gccgccggcc cgggcgagcg cgtgccgccg ctcgaaagag aagcccttgt ccacccagcg
     1861 gcgcaccgcg tcgcgcagag actcctggtc ttcggtgaaa tcgaaatcca tggtcaggct
     1921 cccagcacag tttgcgagat gatgttgcgt tggacttcgt tgctgccgcc gtagatcgtc
     1981 gtcttgcgga cgttgaaata ggtagcggcc aggggcgcgc aatgcatcgc gccgacatag
     2041 tcgccctgcc agccggcctc catcgcctcg tggatgaagg gcagcgaata gggcccggcg
     2101 gccagcatca tcagctcggt gtagcgctgc tggatctcgc tgccgcgtat cttcaggagg
     2161 cccgcgatgt ccagcgcttt cttgccgccc ttttcggccg acagcacgcg cagcaccatc
     2221 atctccagcg cgacgatgtc gacttcgagc agcgcgatct ggtcgcgcaa gcgcgagtcc
     2281 tcgtagacgc cttcggcctt cgcgatgcgc ttcaagcgct ccagctcgcg cttggcgcgg
     2341 ttcacgtcgg cgatgttggt gcgctcgtgg gccagcaggt atttggcata ggtccaaccc
     2401 ttgttctcct cgccgaccag gttgtcggcc ggcacctcga cgttgtcgaa gaagacctcg
     2461 ttgacctcat gctcgccgtc catcgtgatg atgggccgca ccgtgatgcc gggcgatttc
     2521 atgtcgatca gcaggaagct gatgccggtc tggggcttgc cttcattgct ggtgcgcacc
     2581 aggcagaaga tccagtcgcc gtactggccc agcgtggtcc aggtcttctg gccgttgacg
     2641 atgtagtagt cgcctttgcg ctcggcgcgc gtcttgagcg acgcgaggtc cgagcccgag
     2701 cctggctcgc tgtagccctg gctccaccac acctcgccgc tcatgatgcc gggcaggaag
     2761 cgcctgtgct gctcaggaga gccgaaggcc atgatcaccg gcgcgaccat cacgggcccg
     2821 aagggaacca cgcgtggcgc cccggccagc gcgcattcct cctcgaacag atggcgctgc
     2881 actgcattcc agcccgggcc accgaattcc ttgggccagg cccagccgtg ccagcccttc
     2941 ttgccgagga tcttggccca gcgctgcata tcgtccttgg acaagcgcag cgcgttgtgc
     3001 accttgtgcg agatgtcctg cggcagattg gcgcgcaccc aggcgcgaat ctcgtcgcgg
     3061 aaggcgagtt cctcggcggt gaagttcagg tccatgcgtg tgtctccttc acgcctgcgc
     3121 tgcgcactcc atgcgggcgt ggctgtcgag tctacgcagg gcggggctgt ttcgcctgtc
     3181 gctgttgcga caagggtcat cgcgagaccc ttgggagcgc agaggcggcg gcggatcagt
     3241 accttttgtc cagcgtcagc acatcgttct cgcgcttcat ctggaagcgg ccgagaaagc
     3301 tgttgcctag cagcacgacc ggcatgtcgg ccggctgcac cacggcgtcg acgttgtaga
     3361 ccgtgacgtc gttgacgcgc acgctgtcca gcgagacgcg atagccgatg gcctggccgt
     3421 tggcggtgtt gatgcccacg cgctggccgc cctcgtattt caggccgatg cgctgcgcgt
     3481 cggcgaagct catcgcgacc atcgtggccc cggtgtcgac caggaactgc acggcccggc
     3541 cgttgatgct gccgccggcg acgaaatgcc cgcccgggcc ggcgctcagc acgatcttcg
     3601 ttcccccgcc gccgctggtg cccttgccgc ccacgctgac gggcgcgccg cccagtacga
     3661 ggctttggcg ctggcccgca acctcgatca ccgccatgcc gccgctcacg ctgacgagct
     3721 tgacgccctg ggccgattcg ccgggcgcca gcgccctggg cgcgccaccg tcgatgacca
     3781 gcaaggcctt gttgcccata ctgccgctca aggcgacgct ttgggcaaac accggcgcag
     3841 ccgccgccag caatgcaccg cagagcaggg cgcgcatgag gggcctgctc agtcgcggaa
     3901 attgttgaag ctcaacgggg cttccgcgac ttccttgcgg atcagcgcca tcgcggcctg
     3961 caggtcgtct cgcttggcgc ccgtgatgcg caccgtgtcg ccctggatcg cggcctgcag
     4021 cttcatcttg ctctccttga tcagcttctg gatcttcttg gccagctcgc tttcgatgcc
     4081 ggccttgacc ttcacgagct gcttgacctt gtcgccgcca atcttctcga tcttgggggt
     4141 ggtgtcaagg aagcgcacgt cgacattgcg cttgctcagc ttggccgtca ggatatccat
     4201 cacctggccg agctggaact cgctgtcggc gtagatcgtc agctccttgt ccttctgctc
     4261 gatgcgggcg tccgagccct tgaaatcgaa gcgcgtgccg atttccttgt tgctctggtc
     4321 gatggcgttg cgcacctcga cgagattggg ttcgagcacg gtatcgaagc tgggcatggc
     4381 ggtctcgtgt ttaaggcccg ttcgtgtcaa caggtcttgg gaaggcgatc ggcgaaaatt
     4441 ctgccatgca gatcgaaaca ggcgtgagcc tgaagcctta caacaccttc ggcctcaatg
     4501 cagtggccga gaagctggtg cgcatcacga gcgaggccga cgtgcgccgc gtggtcgacc
     4561 accccgagct gggccgtgcg ccgaagttca tcctcggcgg gggcagcaac atcgtgctga
     4621 cgcgcgatgt ggcggcctgc gtgctgaaga tcgaggtcaa gggccggcgg ctgctggggc
     4681 agcgcgacag cgccctcatc gcggaagtcg gcgccggcga gaactggcat gacacggtgg
     4741 cctggacgct ggagcagggt tggcccgggc tggaaaacct ggccctcatc cccggcaccg
     4801 tgggcgccgc gccggtgcag aacatcggtg cctatggcgt cgagctgaag gaccgcttcg
     4861 agtcgctgga tgcggtggac ctcgtcacag gccgcaccgt gacgctgacg cgcgagatgt
     4921 gccacttcgg ctaccgcgac agcgtcttca agcagggcct ggccggcaag agcgtgatca
     4981 cccgcgtgcg cttgcgcctg ccgcagcctt ggctggccca gctgagctat ctggagctgg
     5041 accgcaagcg ccacgagacc ggcatcggca accccagccc gcagcaggtc ttcgactgga
     5101 tctgcgcgat acgccgcgcc aagctgcccg acccggccga gatcggcaac gtcggcagct
     5161 tcttcaagaa cccggtcgtc accgaggagc agtgccgcga catcatcggg cgcgatccgg
     5221 cgatcgtgca ctacccgatg cccgacggca gcgtgaagct ggccgccggc tggctgatcg
     5281 acgcctgcgg ctggaagggc aagtcgatcg gcggggccgg cgtctacgag aagcaggcgc
     5341 tggtgctggt caaccgcggc aatgcctcgg gcggcgaggt ggtgacgctg gcgcgggcga
     5401 tccaggagag cgtctacggc cgcttcggca tacggctgga gcccgagccg gtggtggtgt
     5461 gacgagcgcg gatcaatgtg ggaaggcgag ccgcttttct aacaatttct gcacctgctc
     5521 gaagctcagg ctcaggaccc agtagacgcc ggccgccgcg atgtagagcg gaaagggctg
     5581 gaaggtggtc gaaatcgcct ccttggtcgc cagcatcagc tcggtgacgg cgatcaccga
     5641 gaccagcgag gtgtccttga tcaggctgat cagactgttc gacaggctgg gcaccgcgct
     5701 gcgcaaggcc tgcgggccga tgacatagcg cagcgtctgc acgcgcgtca ggcccaggct
     5761 ggtgccg
//
LOCUS       DASUCY010000607         6989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1395447, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000607 DASUCY010000000
VERSION     DASUCY010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 6989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6989
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395447"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..345
                     /locus_tag="VFV25_12035"
     CDS             82..345
                     /locus_tag="VFV25_12035"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY1394098.1"
                     /translation="MGPRLARWLLMSEDRAHSDGFHLIHEFLAYMLGVRRVGITGAAG
                     DLQHRGLIRYHRGSITVLIRAGLESAACGCYAADRHAYEPLLG"
     gene            complement(683..6904)
                     /locus_tag="VFV25_12040"
     CDS             complement(683..6904)
                     /locus_tag="VFV25_12040"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEY1394099.1"
                     /translation="MKNPLSPLSRGKIFDNLRRSLTPAYLTALLLLGWTVLQPAWLWT
                     LWGVAILFVPPLAALLVDLLRKPASLRAGQHVTATVPSALRQFGQATLTLACLPYEAA
                     FSLDAVLRTLGRLWITRRRLLEWQPSADVALRATPGSAQDLLQTLKTMWVAPALALAT
                     AVALSVVRPEALTAAAPVLALWLLSPVTVWWLGRPLQRRQSTLSAAQHLFLRLLARRT
                     WAFFDTFVGPADHWLPPDNMQEHPVPRIAHRTSPTNIGFSLLANLTAHDFGYLTLGQL
                     IARTSATLDTMEALEKYESHLYNWYDTQSLQPLHPAYVSTVDSGNLAGLLLTLRGGLQ
                     ALAVETPQLSRLFAGFADTVQLLRQALAKPAVGSPLVRFEALLSNAQAAPPQEPHMLW
                     SAFDGLARCAAELLASMGPPPPAPVAGDAADLPAVDEARRWADALDRQCRAALAELEV
                     LSPAAAQPAGPAEAAWDRPALLTRCSVLLHLAARAGALAEMDYGFLYDPARNLMTIGY
                     NVDERRRDSGYYDLLASEIRLCSFVAVAQGKVPQESWFALGRLLTTVDGEPILLSWSG
                     SMFEYLMPMLVMPSYENTLLDQTMRAAVERQIHYGRQRGVPWGMSESGYHATDTALNY
                     QYRAFGVPGLGLKRGLAEDLVVAPYATVMALMVEPRAAYQNLVRLAEEGLAGTYGYYE
                     SIDYTPARLPRGRPGAVVRSFMAHHQGMSLLALVHVLLGQPMQRRFEADPQLQATLLL
                     LQERIPKAVAFHPHTADRAELRPTFGTAETPLRLITTPDTPTPQVQLLSNGRYHVMVT
                     NAGSGYSRWKDLAVTRWREDGTCDPWGSFCYLRDTETGTCWSSAHQPAQSKPDSYEAI
                     FSEGRAEFRRRDQGIDAYTEIVVSPEDDIELRRLRLTNLSRTRRSIELTSYTEVVLAP
                     QAADVQHPAFSKLFVQTELVDDPPALLCTRRPRSADEPAPWMCHLMAVHGAKASAASH
                     ETDRARFIGRGRTLSAPQAMRAASDLSDSAGSVLDPIAATRRVVALEPGQTATVDIVY
                     GMAESRDGCLALLAKYADRRLADRVFELAWTHSQVVLRQLNATEADTELYARLASAVI
                     YSQPTLRADSALLLRNRRSQSGLWGYAISGDLPIVLLQIGDAANIELVRQMVQAHAWW
                     RLKGLAVDLVIWNEERDVYRQRLHEQILGLIAAGVEAHVVDRPGGIFVRHAEQISNED
                     RVLLQAVARVLISDRHGTLAQQVLRKPVGERRAPPFVATRAPEAERNPAPSTSNAVRG
                     LLLFNGTGGFTPNGREYVIAPPAGLCPPAPWVNVLANPRFGCVVSEAGSAYTWCENAH
                     ELRLTPWHNDPVCDSGGEAIYLRDEASGQVWLPTALPTTQHERDDDNPSPYVTRHGFG
                     YSVFEHDEAGIHSELTVYVAMDAAIKFSVLRLRNDGTRERRLSATGYVEWVLGDLRAK
                     TAPHITTDVASDNGALYARNSYSNDFGDWVGFFDVDEPDRLFGTFTCDRAEFIGRNGS
                     LHRPAALARTRLSGRTGAALDPCSAIQVPFDLLPGQTREIVFRLGMGRSADEAGQMVR
                     RFRGSAAAREALDAVHRHWEHALGNVQVHTPDAALNVLANGWLVYQTLACRMWARSGF
                     YQSGGAFGFRDQLQDAMALVHSRPHLLREHLLLCASRQFVEGDVQHWWHPPSGRGVRT
                     HISDDYLWLPLALCRYVHATNDTGVLIEGVPFIDGRPVNPGDDSYYDLPGHSIHTASL
                     YQHAVRALLHALRFGAHGLPLMGGGDWNDGMNLVGHEGRGESVWLGFFLCEVLRQFAT
                     LARGHDDEAFAQRCDDECLQLRERLETHAWDGAWYRRAYFDDGTPLGSAANTECQIDS
                     IAQSWSVLSGVADPERARHAMDALATRLVRAEHGLVQLLDPPFDGHGPNPGYVSGYVP
                     GVRENGGQYTHSAIWAAMAFAALGDHEHAWQLMDIINPLHHGRTASEVEVYKVEPYVV
                     AADVYSVAPHTGRGGWSWYTGSAGWLYRLVLESLLGVQLETTHDGAQLHLAPCLPHDW
                     PGFTLDYRYRDTPYRIEVTQGSAEPGVTLDEVVQVGMALPLVDDGRPHLASVRLASP"
BASE COUNT         1094 a         2319 c         2482 g         1094 t
ORIGIN      
        1 ctagcgtcga tgcacaggat atgtttgtgg ccgccaccga aaatcggctg atcgggctat
       61 gccgcgtcga gcttccacgg catcggacca cgactcgctc gctggttgct gatgagcgag
      121 gatcgggcac attccgacgg cttccacctg atccacgagt ttctggccta catgctgggc
      181 gttcgtcgcg ttggcatcac cggcgccgcc ggcgatctgc agcatcgtgg cctcatccgc
      241 tatcaccgag gcagtatcac cgtgctcatt cgggccggcc tggagtccgc cgcatgtggc
      301 tgctacgcgg cggatcgaca cgcctacgag ccgttgctcg gctgatcgca tgcaacacga
      361 aacccgcacg aggcgggttt cgcagcgagg ctggaagaag gggcagaacc ccttggagca
      421 aggctcccgt ggcgtgatga gccactgagt ccaccagtcc atcgactcat atgtttgacc
      481 ccacgctgca cgcaagatcc catgcctatt agctatcgat gatcgagatg gcggcggcaa
      541 gtccaatggc gcgaacgcga ccatctgtgc gcgcggatgt gcgctacctc taccggtggt
      601 gatcgtgcga acgcagcggt tgcggtcgat gcgctagcac acgatcgcgg ggaacgcgcg
      661 ggtgccgaag gtgatgccgt cgtcacggcg aggcaagccg cacgctcgcc aggtgcggcc
      721 tgccgtcgtc caccaacggc agggccatgc ccacctgcac cacctcatcc agcgtcacgc
      781 caggctcggc ggagccttgc gtcacctcga tgcggtaggg ggtatcgcgg tagcgatagt
      841 cgagtgtgaa gcccggccag tcgtgaggca ggcacggcgc gaggtgcaac tgtgccccgt
      901 cgtgtgtggt ctccagctgc acgcccagca gcgactcgag caccagccgg tacagccagc
      961 ccgccgaccc ggtgtaccag ctccagccgc cacggccggt gtgcggcgct acgctgtaaa
     1021 cgtcggccgc gacgacgtag ggctcgacct tgtagacctc gacttcgctc gccgtgcgcc
     1081 cgtggtgcaa cgggttgatg atgtccatca gctgccatgc gtgctcgtgg tcgcccagtg
     1141 cggcgaaggc catcgcggcc cagatcgcgc tgtgggtgta ctggccgccg ttctcgcgca
     1201 cgcccggcac atagccgctg acgtagcccg ggttggggcc gtggccgtcg aaaggcgggt
     1261 cgagcaattg caccaggccg tgctcggcgc gtaccagccg ggtcgccagc gcgtccatcg
     1321 catggcgcgc ccgctcgggg tcggccacac ccgacagcac cgaccagctc tgcgcgatcg
     1381 agtcgatctg gcactccgtg ttcgccgccg agccgagcgg cgtgccgtcg tcaaagtaag
     1441 cgcggcgata ccaggcaccg tcccacgcgt gcgtttcgag gcgctcgcgc agctggaggc
     1501 actcgtcgtc gcagcgctgc gcgaatgcct cgtcgtcatg gccgcgtgcc agcgtggcga
     1561 actggcgcaa cacctcacac aggaaaaagc cgagccacac gctctcgccg cgaccctcgt
     1621 ggccgaccag gttcatgccg tcgttccaat cgccgccgcc catcagcggc aggccgtgtg
     1681 cgccgaagcg cagcgcgtgc agcaatgcgc gcactgcgtg ctggtagagg ctcgccgtat
     1741 ggatggagtg gccgggcagg tcgtagtagg agtcgtctcc ggggtttacc ggccggccgt
     1801 cgatgaaggg cacgccttcg atcagcactc cggtgtcgtt ggtggcatgc acatagcggc
     1861 acagcgcaag aggcagccac aggtagtcgt cggaaatgtg cgtgcgcacg cctcgtcccg
     1921 acggcgggtg ccaccagtgc tgcacgtcgc cctcgacgaa ctggcggctt gcgcacagga
     1981 gcaggtgttc gcgcagcagg tgcggccggc tgtgcaccag ggccatcgcg tcttgcagct
     2041 gatcacgaaa accgaaggca ccgcccgact gatagaagcc gctgcgggcc cacatgcggc
     2101 aggccagcgt ctggtatacg agccagccgt tggccagcac gttcagcgcg gcgtcggggg
     2161 tgtggacctg cacgttgccg agcgcgtgct cccagtgccg gtgtaccgcg tcaagcgctt
     2221 cgcgcgccgc ggcgctgccg cgaaagcgcc gcaccatctg gccggcctcg tcggcgctgc
     2281 ggcccatgcc gaggcgaaaa acgatctcgc gggtctggcc gggcaacaga tcgaagggca
     2341 cctgaattgc actgcacggg tcgagcgcgg caccggtgcg gcccgacagc cgcgtgcgcg
     2401 caagcgctgc cggccgatgt agcgagccgt tgcgtccgat gaattcggcg cggtcgcagg
     2461 tgaaggtgcc gaacaggcgg tcgggttcgt cgacgtcgaa gaatccaacc cagtcgccga
     2521 agtcgttgct gtaggaattg cgtgcgtaca acgcgccgtt gtcggaggcg acgtcggtgg
     2581 tgatgtgcgg cgccgtcttg gcgcgcaggt cgcccaacac ccactccaca tagccggtgg
     2641 ccgacaggcg ccgctctcgc gtgccgtcgt tgcgcagccg cagcaccgag aatttgatgg
     2701 ctgcgtccat ggccacgtag acagtgagtt cgctatggat gcccgcctcg tcgtgctcga
     2761 acacgctgta accgaagcca tggcgcgtga catagggcga tgggttgtcg tcgtcgcgtt
     2821 catgctgggt cgtgggcagc gcggtgggga gccagacctg cccgctcgct tcgtcgcgca
     2881 ggtagatggc ttcgccgccg ctgtcgcaca ccgggtcgtt gtgccacggc gtgaggcgca
     2941 gctcgtgggc gttttcgcac caggtgtagg cgctgcccgc ctccgaaacc acgcagccga
     3001 agcgtgggtt ggcgagcacg ttgacccacg gcgccggcgg gcaaaggccc gcgggcgggg
     3061 cgatgacgta ctcgcgcccg ttgggcgtga agccgccagt gccgttgaag agcagcagcc
     3121 cgcgcacagc gtttgacgtg ctcggcgcag ggttgcgctc cgcctcgggg gcacgcgtgg
     3181 caacgaaagg tggggcgcgg cgctcgccca ccggcttacg cagcacctgt tgcgcaagcg
     3241 tgccgtggcg gtcactgatg agcacgcgcg ccaccgcctg cagcagcacg cggtcttcgt
     3301 tcgagatttg ttccgcatgg cgcacgaaga tgccgcccgg tcggtcgacc acatgcgcct
     3361 cgacgccggc cgcgatgagc ccgaggatct gctcgtgcag ccgctggcgg tatacgtcgc
     3421 gttcttcgtt ccagatcacc aggtcgaccg ccaggccctt caatcgccac cacgcatggg
     3481 cctgcaccat ctgccgcacc agctcgatgt tggcggcgtc gccgatctgc aggagcacga
     3541 tcggcaggtc gcccgagatg gcgtagcccc acaggcccga ctggctgcgc cggttgcgca
     3601 gcagcagcgc gctgtcggcg cgcagcgtcg gctgcgaata gatcacggcg ctggccagcc
     3661 gcgcatacag ctcggtgtcg gcttcggtcg cgttgagttg gcgcagcacg acctggctgt
     3721 gcgtccacgc gagttcgaac acgcggtcgg cgaggcgtcg gtcagcgtac ttggcgagta
     3781 gcgcaaggca gccgtcgcgc gactcggcca tgccgtagac gatgtcgacg gtggcggttt
     3841 gtcccggctc gagcgcgacg acgcggcgcg tggcggcgat ggggtcgagc accgagccgg
     3901 cactgtccga caagtctgag gcagcgcgca tcgcctgcgg cgcgctgagg gtgcggccgc
     3961 ggccgatgaa gcgtgcgcgg tcggtctcgt gcgatgccgc gctcgctttc gcgccgtgca
     4021 ccgccatcag gtggcacatc cacggcgccg gctcgtcggc cgagcgcgga cggcgcgtgc
     4081 acagcagggc gggcggatcg tcgaccagct cggtctgcac gaacagcttg ctgaacgccg
     4141 ggtgttgaac gtcggccgct tgcggcgcca ggactacctc ggtgtagctg gtgagctcga
     4201 tgctgcgccg tgtgcggctc aggttggtca ggcgcaggcg gcgcaactcg atgtcgtctt
     4261 ccggcgagac gacgatctcg gtgtaggcgt cgatgccttg gtcacggcgc cggaactcgg
     4321 cgcggccctc ggagaagatg gcttcgtagg agtcgggctt cgattgggcg ggttggtgcg
     4381 cgctcgacca gcaggtgccg gtttcggtgt cgcgcaggta gcagaagctg ccccaggggt
     4441 cgcaggtgcc gtcttcacgc cagcgcgtca cagccaggtc tttccagcgg ctgtagccgc
     4501 tgcccgcatt ggtgaccatc acgtggtagc gtccgttgga cagcagctgc acctgcggcg
     4561 tcggcgtgtc gggcgtggtg atcaggcgca gcggcgtctc ggccgtgccg aaggtcgggc
     4621 gcagttcggc gcggtcggcg gtgtgtggat ggaaggccac ggccttcggg atgcgctcct
     4681 gcagcagcag cagcgtggcc tgcaactgcg ggtcggcctc gaagcggcgc tgcattggct
     4741 ggccgagcag cacgtgcacc agcgccagca gactcatgcc ctgatggtgg gccatgaacg
     4801 aacgcaccac cgcgccgggg cgcccacgcg gcaaccgcgc cggcgtgtag tcgatggatt
     4861 cgtagtagcc gtaggtgccc gccagcccct cttcggccag ccgcacgagg ttctggtagg
     4921 cggccctggg ctcgaccatc agggccatca cggtcgcata aggcgccaca accaggtctt
     4981 cggccagccc gcgcttcagg cccaggccgg gcacgccgaa ggcgcggtac tggtagttca
     5041 gcgcggtgtc ggtggcgtgg tagcccgact ccgacatgcc ccacggaacg ccacgctggc
     5101 ggccgtagtg gatctgccgc tccaccgccg cgcgcatggt ctggtcgagc agcgtgttct
     5161 cgtagctggg catcaccagc atcggcatca ggtactcgaa catcgaaccg ctccacgaca
     5221 gcaggatcgg ctcgccgtcg acggtggtga gcaggcgtcc gagcgcgaac cagctctcct
     5281 gcggcacctt gccctgggcc acggcgacga agctgcacag gcgaatctcc gaggccagca
     5341 ggtcgtagta gcccgagtcg cggcgccgtt cgtcgacgtt gtagcctatg gtcatcaggt
     5401 tacgcgccgg gtcgtagagg aagccgtagt ccatctcagc cagcgcgccg gcgcgagcgg
     5461 ccaggtgcag cagcaccgag cagcgcgtga gcaacgccgg cctgtcccag gccgcttcgg
     5521 cggggccggc gggctgcgct gccgcgggcg acagcacctc tagctcggcc agcgcggcgc
     5581 ggcattggcg gtcgagcgcg tcggcccagc gccgcgcttc gtcgaccgct ggcagatcgg
     5641 cggcgtcgcc ggccaccggt gcagggggtg gcggccccat cgatgccagc agctcggccg
     5701 cgcagcgtgc caagccgtcg aacgccgacc agagcatatg cggttcctgc ggtggcgcgg
     5761 cttgtgcatt cgacagcagc gcctcgaagc ggaccagcgg gctaccgacc gcaggcttgg
     5821 ccaaagcttg ccgcagcaac tgcacggtgt ctgcgaaccc cgcgaacagg cgcgacaact
     5881 gcggcgtctc gacagccagt gcctgcagcc cgccgcgcag cgtcaacagc aggccggcga
     5941 ggttgccgct gtccaccgtc gatacgtagg ctgggtgcag cggctgcagc gactgcgtgt
     6001 cgtaccagtt gtagagatgg ctctcgtatt tttccagcgc ctccatcgtg tcgagcgtgg
     6061 cgctggtgcg tgcaatcaac tgcccgagcg tgaggtagcc gaagtcgtgc gccgtcaggt
     6121 tggcgagcag cgagaagccg atgttggtcg gcgaggtgcg gtgcgcgatg cgtggcaccg
     6181 gatgctcctg catattgtcg ggcggcagcc agtggtcggc gggcccgacg aaggtgtcga
     6241 agaaggccca ggtgcggcgc gccagcaggc gcaggaacag gtgctgtgcg gcgctgaggg
     6301 tcgattgcct gcgctgcagc gggcggccca gccaccacac ggtgaccggc gacagcagcc
     6361 acagcgccag caccggtgcg gcggcggtga gcgcctcggg ccggaccacg cttaaagcca
     6421 ccgccgtggc cagtgccagc gcgggcgcta cccacatcgt cttcagcgtc tgcagcaggt
     6481 cctgcgcgct gcccggtgtg gcgcgcaggg ccacatcggc cgacggttgc cactcgagca
     6541 gccggcgccg tgtgatccac agccggccca gcgtgcgcag cacggcgtcg aggctgaacg
     6601 ccgcttcgta aggcaggcag gcgagggtga gagtggcctg gccgaactgc cgcaaggccg
     6661 acggcaccgt ggccgtcaca tgctggcccg cgcgcagcga cgcgggcttg cgcaacaagt
     6721 caaccagcaa cgcagcgagc ggcggcacga acaggatggc gacgccccac agcgtccaca
     6781 gccaggctgg ttgcagcacc gtccaaccga gaagcagcag cgcggtgagg taggcaggcg
     6841 tgaggctgcg ccgcaggttg tcgaagatct tcccgcgcga cagcggcgac agcgggttct
     6901 tcacggttac cgcatgccca ctgccttgcg cggcatcgtg cggcaggcgc agcttgggcc
     6961 agagccacgc gatgatctgc cagtcgccg
//
LOCUS       DASUCY010000608         2074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_752820, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000608 DASUCY010000000
VERSION     DASUCY010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752820"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="VFV25_12045"
     CDS             <1..676
                     /locus_tag="VFV25_12045"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEY1394100.1"
                     /translation="KKVHLKSILLELLWFLRGDGNARWLQERGVTIWDEWASADGDLG
                     PVYGVQWRSWPKPDGGHIDQIAEVVKQLKTNPDSRRIIVSAWNVADLPKMALMPCHAF
                     FQFYVAPAESPAARGRLSCQLYQRSADIFLGVPFNIASYALLTHMLAQQCDLDVGDFI
                     WTGGDCHIYGNHFEQVETQLARTPYPYPVLNIQRRPASIFDYEYEDFEVLDYQHHAAI
                     KAPVAV"
     gene            691..1197
                     /locus_tag="VFV25_12050"
     CDS             691..1197
                     /locus_tag="VFV25_12050"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEY1394101.1"
                     /translation="MKFALIAAVARNGVIGKDNNLLWRLPEDMRHFKATTLGCPVLMG
                     RKTFDSIGKPLPGRRNLVITRNVEWLAPGVEVATSLHDALAVLHDATEVFVIGGAQIY
                     AEMLPYADELVLTEIDADFVGDTHFPDWDRSAFEEVSRETHHAAPPNDFDFAFVTYRR
                     KEPRSFDV"
     gene            1190..1768
                     /locus_tag="VFV25_12055"
     CDS             1190..1768
                     /locus_tag="VFV25_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HEY1394102.1"
                     /translation="MSENGFHVHGPHDHAVEHAAEHGGGDSFSGQIAVMTAILATVGA
                     LFSYMGGATQADAGLYKNNAAIKKTEAADQWNYYQSKGNKQNLAELAAAIVPAEKQEA
                     YRKEVERYKSEKEAIKKDADKLEAESKEWDEKSEAKMHLHHRWAQATTALQISIAMAA
                     IALLTRRKWLEWGMFGMSGIGLLLGIAALLHW"
     gene            1782..>2074
                     /locus_tag="VFV25_12060"
     CDS             1782..>2074
                     /locus_tag="VFV25_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEY1394103.1"
                     /translation="MKLATWNVNSLAVRLPQLIEWLAANPVDAIVLQETKLTDDKFPA
                     AELQAAGYEAQWFGQKTYNGVALLTRAGQVDGVVRNIPGFADEQARLIAGSVAG"
BASE COUNT          393 a          694 c          668 g          319 t
ORIGIN      
        1 caagaaggtc caccttaagt ccatcctcct cgagctcttg tggttcctgc gcggcgacgg
       61 caacgcccgc tggctgcagg agcgcggcgt gacgatctgg gacgaatggg cgagcgccga
      121 cggcgacctg gggccggtct acggcgtgca atggcgcagc tggcccaagc ccgatggcgg
      181 ccacatcgac cagatcgccg aggtcgtcaa gcagctcaag accaaccccg actcgcgccg
      241 catcatcgtg agcgcctgga acgtggccga cctgccgaag atggcactga tgccctgcca
      301 cgccttcttc cagttctatg tggcgcccgc cgaatcgcct gcggctcggg gccgcctctc
      361 gtgccagctc taccagcgca gcgcagacat cttcctcggc gtacccttca acatcgcgag
      421 ctatgcgctg ctgacccata tgctggccca gcaatgcgac ctggacgtcg gcgacttcat
      481 ctggaccggc ggcgactgcc acatctacgg caaccacttc gagcaggtcg agacccagct
      541 cgcgcgcacg ccctacccct atccggtgct gaacatccag cgccgtccgg catccatctt
      601 cgactacgaa tacgaggact tcgaggtgct ggactaccag caccatgcgg ccatcaaggc
      661 gccggtcgcc gtatgaggaa ggcggccggc atgaagttcg ccctgatcgc ggcagtggcg
      721 cgcaacggcg tcatcggcaa ggacaacaac ctgctgtggc gcctgcccga agacatgagg
      781 cacttcaagg cgacgaccct gggctgcccg gtgctgatgg ggcgcaagac cttcgactcg
      841 atcggcaagc cgctgccggg gcggcgcaac ctcgtgatca cgcgcaatgt cgagtggctg
      901 gcgcccggcg tcgaggtggc gacctcgctg cacgacgcgc tggccgtgct gcacgacgcc
      961 accgaggtct tcgtgatcgg cggcgcgcag atctatgccg agatgctgcc ctatgccgac
     1021 gagctggtgc tgaccgagat cgatgccgat ttcgtcggcg acacgcactt cccggactgg
     1081 gaccgcagcg ccttcgagga ggtctcgcgc gagacccatc acgccgcgcc gcccaatgat
     1141 ttcgatttcg ccttcgtcac ttaccgaaga aaagaaccaa ggagcttcga tgtctgagaa
     1201 cggtttccac gtccacggcc cgcacgacca cgcggtggag catgccgccg agcacggcgg
     1261 gggcgattcc ttctccggtc agatcgccgt gatgacggcc atcctggcca cggtaggcgc
     1321 gctcttctcc tacatgggcg gcgccaccca ggccgacgcg gggctctaca agaacaacgc
     1381 ggcgatcaag aagaccgagg ccgccgacca gtggaactac taccagtcca agggcaacaa
     1441 gcagaacctg gccgaactgg ccgcggccat cgtgcccgcc gagaagcagg aggcctatcg
     1501 caaggaggtc gagcgctaca agagcgagaa ggaagcgatc aagaaggacg ccgacaagct
     1561 cgaggccgag tccaaggaat gggacgagaa gtctgaggcc aagatgcatc tgcaccaccg
     1621 ctgggctcag gccaccacgg ccctgcagat ctccatcgcg atggccgcga tcgcgctgct
     1681 gacgcgccgc aagtggctgg agtggggcat gttcggcatg agcggcatcg gcctgctgct
     1741 gggcattgcg gcgctgctgc attggtgagc tgcgagggca catgaagctc gccacctgga
     1801 acgtcaattc gctcgccgta cggctgcccc agctgatcga atggctcgcc gccaacccgg
     1861 tcgatgcgat cgtgttgcag gagaccaagc tcaccgatga caagtttcct gccgccgagc
     1921 tgcaggcggc aggctacgag gcgcagtggt tcggccagaa gacctacaac ggcgtggcct
     1981 tgctgacgcg cgcggggcag gtcgacggcg tggtgcgcaa cattcccggc ttcgccgacg
     2041 aacaggcgcg cctgatcgcc ggcagcgtgg ccgg
//
LOCUS       DASUCY010000609         7675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1202710, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000609 DASUCY010000000
VERSION     DASUCY010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 7675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7675
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202710"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /gene="rapZ"
                     /locus_tag="VFV25_12065"
     CDS             complement(<1..918)
                     /gene="rapZ"
                     /locus_tag="VFV25_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEY1394104.1"
                     /translation="MTATARKPREATAPKDVREVVLVSGISGSGKSVALAALEDAGFF
                     CVDNLPPQLLREFIKLEHERSARRVAVGVDVHTSGSMAHLLPVLEQLRRDGVAVRSIF
                     LDASTDTLVRRFSETRRRHPLSDDFSEAGIETSSEDAHRVLTEAIRLERELLADLREA
                     STVIDTSQLRPAQLRAWMRNLVQTSASRLTLVFESFAFKNGVPLDADYVFDVRMLPNP
                     HYVRELKPQTGRDPDVIAYLEAQPEVFEMLAQIESFLARWLPAIEVDQRNYLTVAIGC
                     TGGQHRSVYFAEQLARRFAARGITLVRHRE"
     gene            complement(915..2618)
                     /gene="recN"
                     /locus_tag="VFV25_12070"
     CDS             complement(915..2618)
                     /gene="recN"
                     /locus_tag="VFV25_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEY1394105.1"
                     /translation="MLRRIALRDFVIVTELEVELGDGFSVLTGETGAGKSILVDALQL
                     ALGSRGDAGVVREGAQRAEITAEFDLAAPLADWLEAAGFERNGEDSLLLRRSLDAQGK
                     SRAWINGSAATVSQLRELGELLVDIHGQHAWQSLTRADSVRGLLDAYAGVDLAPLSAA
                     WSTWRAARQKLDDARGRQADLERERERLQWQIAELDKLAPAEGEWPELNAEHARLAHA
                     QAILDAAQAALDAVSEGEAAADGLVGRAIEALQPVAGYDATLGNALEVLQAAQAQLQD
                     AAHSLNAALRHTDLDPERLQSLDERLAAWMGAARRYRRLPEELPATLQAWRGELKSLE
                     AATDLDALERELAAAQQAYETAAKSVSKLRRAAAPKLSQAVTAAMQQLGMAGGRFEAA
                     LAAAPEPQAHGLESAEFLVAGHAGSTPRPLVKVASGGELSRIALAIAVTTSELGEAGT
                     LIFDEIDAGVGGAVAETVGRLMKQLGRDRQVLAVTHLPQVAACADHHHVVAKTSKPAG
                     TTSDVQSVAGEARVAEIARMLGGQQLSGAGLAHAKEMLATAAATRSAAGGSKAGRRSS
                     S"
     gene            complement(2618..3496)
                     /locus_tag="VFV25_12075"
     CDS             complement(2618..3496)
                     /locus_tag="VFV25_12075"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEY1394106.1"
                     /translation="MSTRFRHAALVGKYQAQGSRSGLDEIAQFLARQGLEVSLERETA
                     LNTGLTDYPALSPAELGAQCDLAVVVGGDGTMLAIARELARYDVPLVGINQGRLGFIT
                     DIPVSEVAATLAPMIAGDFEEEQRTMLEGAVERDGRVIYEGFAFNDVVVSRGATASMV
                     ELKVEVDGQFVANLRADGVIIASPTGSTAYSLSAGGPILHPSIPGWLLVPIAPHTLSN
                     RPIVLPDAGSIRIEVVAGRDASVNFDMQSLASLLHGDQITVRRGAHRVRFLHPRGWSY
                     YATLRRKLHWNAGVVG"
     gene            3567..4595
                     /gene="hrcA"
                     /locus_tag="VFV25_12080"
     CDS             3567..4595
                     /gene="hrcA"
                     /locus_tag="VFV25_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEY1394107.1"
                     /translation="MLDERAKSLLKTLVERYIADGQPVGSRTLSRASELELSPATIRN
                     VMADLEELGLIASPHTSAGRIPTARGYRLFVDTMLTARALQHLDEASLPPDARGQLQP
                     DQPQRVIANAAHVLSELSQFVGVVTAPRKPSVFHHIEFMRLGERRILLILVAPDGDVQ
                     NRVIFTAQDYTQTQLIEASNFLNAHYAGLTIESVRERLKGEIDVLRGEIAVLMQAAVQ
                     IGSEALAESSEQVVVSGERNLLTVQDFGNDMGNLRKLFELFEQKTQLMRLLDISSRAE
                     GVRIYIGGESQVVPFEELSVVSAPYVVDGQVIGTVGVIGPTRMAYDRMIQIVDITARL
                     VGNALSQK"
     gene            4659..4735
                     /locus_tag="VFV25_12085"
     tRNA            4659..4735
                     /locus_tag="VFV25_12085"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4693..4695,aa:Ile,seq:cat)
     regulatory      4908..4994
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            5221..5568
                     /locus_tag="VFV25_12090"
     CDS             5221..5568
                     /locus_tag="VFV25_12090"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEY1394108.1"
                     /translation="MNQIIPSTYKRRVGPRRALRSALVLTLPGNKLLKGEAWDLGEDG
                     VGLVCSKPVSPGTACRVTLDLPYEGQSIAVKAAVKIVYSSYLGPEKFKLGAVFSRLDE
                     ESSRLIREFVSGG"
     gene            complement(5615..6535)
                     /locus_tag="VFV25_12095"
     CDS             complement(5615..6535)
                     /locus_tag="VFV25_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY1394109.1"
                     /translation="MSDSEPNWEWYRTFLQVLETGSLSAAGRAMGLTQPTVGRHIDSL
                     EAALGLKLFTRSFDGFAPTDAAHELRPYAAGIAATAAALRRVASSHGSGVRGTVRLTA
                     SEVIGVEVLPPILAALRNEHPELIVELVLSNRVDDLLHREADIAVRMFRPVQDALVAK
                     RVGGIEVGLHAHESYLAVHGVPKSMSELSRHALIGFDQENAFIRSLQDKFKAFSRDSL
                     AFRADSDLAQLGAIRAGFGIGACQSALAARDKRLVRVLEGQFSLTMETWVAMHEDLRE
                     SARCAATFAALAAGLRSYIQQEGAGARGRP"
     gene            6644..7612
                     /locus_tag="VFV25_12100"
     CDS             6644..7612
                     /locus_tag="VFV25_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEY1394110.1"
                     /translation="MSKVALFGAAGAIGQSIAAALRSKGRPYRVVGRNEQGLRKAFGA
                     DPLAEIVTWNPDSPASVQAAAGGIETLIYLVGVNYWQFELHPELMRKTLDGAVAVGVK
                     NIILIGTVYPYGRPLSKPVREDHPRAPHTFKGQMRKAQEDVLMQAHADGRINATVLRL
                     PDFYGPGVEASLLHRAAQAAVNGGTADMIGPIDRPHEFVFVPDVGPVVARLIETPAAF
                     GKIWHLAGAGVTTQRDLVSEMERQTGHKLKLRVAGKTMLRLVGLFNPFVRELVEMNYL
                     MTEPVIMDDSALQRLIGPVHKTPYAEGIRQTLAALQKTQSLAAATA"
BASE COUNT         1243 a         2677 c         2542 g         1213 t
ORIGIN      
        1 ctcgcgatgg cgcacgagag tgatgccgcg cgccgcgaag cggcgcgcga gctgttcggc
       61 gaaatagacg gaacggtgct ggccgccggt gcagcctatg gccacggtga gatagttgcg
      121 ctggtccacc tcgatggcgg gcagccagcg cgccaggaag ctctcgatct gggccagcat
      181 ctcgaacacc tcgggctggg cttcgagata ggcgatcacg tcggggtcgc ggccggtctg
      241 gggcttgagc tcgcgcacat agtgcgggtt gggcagcatg cgcacgtcga agacatagtc
      301 ggcgtccagc ggcacgccgt tcttgaaggc gaaggactcg aacaccagcg tgagccggga
      361 tgcgctggtc tgcaccaggt tgcgcatcca ggcgcgcagc tgggccgggc gcagctggct
      421 ggtgtcgatc acggtcgagg cctcgcgcag gtcggccagc aactcgcgct ccagcctgat
      481 ggcctcggtc agcacgcgat gggcgtcctc gctgctggtc tcgatgcccg cttcgctgaa
      541 atcatcggac agcggatggc ggcggcgcgt ctctgaaaag cgccgcacca gcgtgtcggt
      601 gctggcatcc aggaagatgg agcgcacggc cacgccgtcg cgccgcagct gctccagcac
      661 cggcagcaga tgggccatgg agccggaggt gtgcacgtcc acgcccaccg ccacgcggcg
      721 cgccgagcgc tcgtgctcca gcttgatgaa ttcgcgcagc agctgcggcg ggaggttgtc
      781 gacgcagaag aagccggcat cctccagcgc cgccagcgcc accgacttgc ccgagcccga
      841 gatgcccgag acgagcacga cctcgcgcac gtccttgggc gcagtggcct ccctgggttt
      901 cctggctgta gctgtcatga ggaggaacgt cgccccgcct tggacccgcc ggcggcggat
      961 cgggttgcgg cggcagtggc cagcatctcc ttggcgtgcg cgaggcccgc gcccgagagc
     1021 tgctggccgc ccagcatgcg tgcgatctcg gccacgcgcg cctcgccggc cacggactgg
     1081 acgtcgctcg tcgtgcccgc gggcttgctg gtcttggcga cgacatgatg gtgatcggcg
     1141 caggccgcca cctggggcag atgcgtcacg gcgagcacct ggcggtcgcg cccgagctgc
     1201 ttcatcaggc ggcccaccgt ctcggccacc gcgccgccca cgccggcgtc gatctcgtcg
     1261 aagatcaacg tgcccgcctc gcccagctcg ctggtggtga cggcgatggc cagcgcgatg
     1321 cgcgacagct cgccgcccga ggccaccttg actagcgggc gcggcgtgct gcccgcatgg
     1381 ccggcgacca ggaattcggc cgactccagg ccatgggcct ggggctcggg cgccgcggcg
     1441 agcgcggcct cgaagcgtcc gccagccatg ccgagctgct gcatcgccgc cgtcacggcc
     1501 tggctcagct tgggcgctgc cgcgcggcgc agcttggaaa ccgacttggc cgcggtctcg
     1561 taggcctgct gtgcggcggc cagctcacgc tccagcgcgt cgagatcggt cgccgcctcc
     1621 agggacttca gctcgcctcg ccaggcctgc agcgtggccg gcagttcctc gggcaggcgg
     1681 cggtagcggc gcgcggcgcc catccacgcg gcgaggcgct cgtcgaggct ttgcaggcgc
     1741 tcaggatcga gatcggtgtg gcgcagcgcc gcattgaggc tgtgcgcggc gtcctgcagc
     1801 tgggcctggg cggcctgcag cacttccagc gcattgccca gcgtcgcgtc atagcccgcc
     1861 accggctgca aggcctcgat ggcccggccg accaggccat cggccgcggc ctcgccctcg
     1921 gacacggcat ccagcgccgc ctgcgcagca tcgaggatgg cctgggcatg agcgaggcgg
     1981 gcatgctcgg cattgagttc cggccactcg ccctctgccg gcgcgagctt gtccagctcg
     2041 gcgatctgcc attgcaggcg ttcgcgttcg cgctccagat cggcctgcct gccgcgggca
     2101 tcgtccagct tctggcgggc cgcgcgccac gtgctccagg cggcgctcaa gggggcgaga
     2161 tcgacgccgg cataggcatc gagcaggccg cgcaccgagt cggcgcgcgt cagactctgc
     2221 caggcgtgct ggccgtggat gtcgaccagc agttcgccca gctcgcgcaa ctgagacacg
     2281 gtcgccgcac tgccgttgat ccaggcgcgg ctcttgccct gggcatccag cgagcgacgc
     2341 agcagcaggc tgtcttcgcc gttgcgctcg aagcccgccg cttccagcca gtccgccagc
     2401 ggcgcggcga ggtcgaactc ggccgtgatc tcggcgcgct gcgcgccttc gcgcaccacg
     2461 ccggcgtcgc cacggctgcc cagggcgagc tgtagcgcgt cgacgaggat ggacttgccc
     2521 gcgccggtct cgccggtcag caccgagaag ccatcgccca gctccacctc gagctcggtg
     2581 acgatgacga agtcgcgaag ggcgatgcgg cgcagcatca gcccaccacc ccggcgttcc
     2641 agtgcagctt gcggcgcagc gtggcgtagt agctccaccc gcgcgggtgc aggaagcgca
     2701 cgcgatgcgc gccgcgccgc accgtgatct ggtcgccatg cagcaggctg gcgaggcttt
     2761 gcatgtcgaa attgacgctg gcatcgcgcc cggccacgac ctcgatcctg atgctgccgg
     2821 cgtccggcag cacgatgggg cggttggaca gggtatgcgg tgcaatgggc accagcagcc
     2881 agccgggaat cgacgggtgc aggatgggcc cgccggcgga cagcgaatag gccgtggagc
     2941 cggtgggcga cgcgatgatc acgccgtcgg cccgcagatt ggcgacgaac tgcccgtcga
     3001 cctccacctt gagctcgacc atgctggccg tggcgccgcg gctcacgacc acgtcgttga
     3061 acgcgaagcc ctcgtagatc acgcgcccgt cccgctccac cgcgccttcg agcatggtgc
     3121 gctgctcttc ctcgaaatcg ccggcgatca tcggcgccag cgtggcggcc acctcgctga
     3181 ccgggatgtc ggtgatgaaa cccagacggc cctgattgat gcccaccagc ggcacgtcgt
     3241 agcgcgccag ctcacgggcg atggcgagca tggtgccgtc gccgcccacg acgacggcca
     3301 agtcgcattg ggcgccaagc tcggccgggc tcaacgccgg gtaatcggtg aggccggtgt
     3361 tgagcgccgt ctcgcgctcc agcgacactt ccaacccctg ccgggccagg aattgcgcaa
     3421 tctcgtccag ccccgagcgg ctgccctggg cctggtattt acctacgagt gcggcgtgac
     3481 gaaagcgcgt cgacatagtg aaggaattac accatagcga ccacgcggga aattgcggca
     3541 ccttcagcac aaatacaatg attttcatgc tggacgagcg tgccaaatcg ctgctgaaga
     3601 ccctggtcga gcgctatatc gccgacggcc agccggtggg ctcgcgcacg ctgtcgcgcg
     3661 cctccgagct ggagctgtcg ccggccacca tacgcaacgt gatggccgac ctggaagaac
     3721 tcggcctgat cgcgagcccc cacacctcgg ccggccgcat ccccacggcg cgcggctatc
     3781 gcctcttcgt cgacaccatg ctgacggcgc gcgcgctgca gcacctggac gaggccagcc
     3841 tgccgcccga cgcacgcggc cagctgcagc ccgaccagcc gcagcgcgtg atcgccaatg
     3901 cggcgcacgt cctgtccgag ctgtcgcagt tcgtcggcgt ggtcaccgcg ccgcgcaagc
     3961 cctcggtctt ccaccacatc gagttcatgc gcctgggcga gcgccgcatc ctgctcatct
     4021 tggtcgcccc ggacggcgac gtgcagaacc gcgtgatctt cacggcccag gactacacgc
     4081 agacccagct catcgaggcc agcaacttcc tgaacgccca ttacgccggc ctcaccatcg
     4141 agtctgtgcg cgagcggctc aagggcgaga tcgatgtgct gcgcggcgag atcgcagtcc
     4201 tgatgcaggc tgccgtgcag atcggctccg aggccctggc cgagagcagc gagcaggtcg
     4261 tcgtctcggg cgagcgcaac ctgctgaccg tgcaggactt cggcaacgac atgggcaacc
     4321 tgcgcaagct cttcgagctc ttcgagcaga agactcagct gatgcgcctg ctcgacatct
     4381 ccagccgcgc cgagggcgtg cgcatctaca tcggcggcga gagccaggtc gtgcccttcg
     4441 aagagctgtc ggtcgtctcg gcgccctatg tcgtggacgg ccaggtcatc ggcacggtcg
     4501 gcgtcatcgg ccccacccgc atggcctacg accgcatgat ccagatcgtc gacatcacgg
     4561 cacggctggt gggcaacgcg ctgagccaga agtaggcgcc ctgccgcgcc tacaatgcct
     4621 gccctgcgcc gcgtggccgg tgcaaaaagc aaacccgagg gccgttagct cagttggtta
     4681 gagcagagga ctcataatcc tttggtcgca ggttcaagtc ctgcacggcc taccatccaa
     4741 atcaacggct taggtactgc ctaggccgtt ggcttttctg gcgtagcccc cgcgtagccg
     4801 ccgaacacat cgagccgatt cgcggccctt gcagtgcttg accagccact cgaaccctgt
     4861 ggcgtcaacg agcgcagcgc tgcgtaactt gcctatacaa tcggcgcgct tgacaaaggc
     4921 acacctgtgc gaaagccggg gtcgcaaagc ttccggtcta cggcccatag ggtcacgata
     4981 gcggggttgc ccacagacga tcggccctgc aagccggtcg tcgtcgcccc tcgggtgact
     5041 cggcaagacg gatatgccgt ccggcgactc caccgcacgc tgtgttcctg cgttcgatca
     5101 gaagctcagg agtcgccacg tgtcagatca tcccagagaa gttgacttcc agcgtcgagg
     5161 ccggcttggt ctaggcctgc gactcggtgc gtcccggcgc gacagcgtga ggcgaccgtg
     5221 atgaatcaga tcatcccgag tacttacaag cggcgtgtgg ggccacgccg ggccctgcgc
     5281 tcggcgctcg tcttgacgct gcccggcaat aagctcctga agggcgaagc ctgggacctg
     5341 ggcgaggacg gcgtcgggct ggtgtgttcg aaaccggtct ctcccgggac ggcatgccgg
     5401 gtcacgctcg atctgcccta tgagggccaa agcatcgccg tgaaggctgc cgtcaagatc
     5461 gtgtactcgt cctacctggg ccctgaaaaa ttcaagctgg gcgcggtctt ctcacggctg
     5521 gacgaagaga gctcgcgcct catccgggag tttgtgtccg gcggctgagg ccatgtggcc
     5581 cgccaccttg cgctcttgct tgctttagga ccgcctaggg ccggccacgg gcccctgccc
     5641 cttcctgttg aatgtaggaa cgcagccccg cagcgagcgc tgcgaacgtc gctgcgcagc
     5701 gggcgctctc gcgcaagtcc tcgtgcatgg ccacccaggt ctccatcgtc aacgagaact
     5761 ggccctccaa gacccggacc agccgcttgt cccgcgcggc cagcgccgac tggcatgcac
     5821 cgatgccgaa accggctcgt atcgctccca gttgggcgag gtcgctgtcg gcccgaaacg
     5881 cgaggctgtc ccgcgagaac gccttgaact tgtcctgcaa gctgcgtatg aacgcgttct
     5941 cctgatcgaa gccgatcaac gcgtggcgcg acagttcgct catcgacttc ggcacgccgt
     6001 gcaccgccag gtaactttca tgggcatgca ggccgacttc gatcccgccg acgcgcttgg
     6061 cgaccaacgc gtcctgcacc ggcctgaaca tccggaccgc gatgtccgcc tcgcggtgga
     6121 gcaggtcgtc cacccggttc gacaagacca gctcgacgat cagctccggg tgctcgttgc
     6181 gcagcgccgc gaggatgggc ggcagcacct cgacgccgat gacttcgctc gccgtcagcc
     6241 gcaccgtgcc gcgaacaccc gatccgtggc tgctggccac gcggcgaagc gcggctgccg
     6301 tggccgcgat gcccgcggca tagggtctca gctcatgcgc agcgtcggta ggcgcaaagc
     6361 cgtcaaacga gcgggtgaag agcttcagcc ccagggcagc ctcgagactg tcgatatgac
     6421 ggccgacggt cggctgcgtc aggcccatgg cgcgcccggc cgccgacagc gagcctgtct
     6481 ccagcacctg caggaaggtc cggtaccact cccagtttgg ctcgctgtcg ctcatggtat
     6541 gcaaagtctt ttagcggcta actaattcta ggcaattttc tttttgccta cgggcctgca
     6601 aactgagctc actctcagct cccacctgca aaggggaacc gccatgtcca aagtcgcact
     6661 gttcggcgcc gccggcgcca tcggtcaaag catcgcagcc gctctgcgca gcaagggccg
     6721 cccgtatcgc gtggtcggcc gcaatgagca gggcctgcgc aaggcctttg gcgccgaccc
     6781 gcttgccgag atcgtcacct ggaacccgga ctcgccggcc tcggtgcaag ccgctgccgg
     6841 cgggatcgag acgctgatct acctggtcgg cgtcaactac tggcagttcg aactgcaccc
     6901 cgagctgatg cgcaagacgc tggatggcgc cgtggccgtc ggcgtcaaga acatcatcct
     6961 gatcggcacc gtctacccct acggtcgccc attgagcaag ccggttcgcg aagaccatcc
     7021 gcgcgcgcca cacaccttca agggccagat gcgcaaggcc caggaggatg tgctgatgca
     7081 ggcccatgcg gacggccgca tcaacgccac cgtgttgcgg ctgccggact tctacggccc
     7141 cggtgtggaa gccagcctgc tgcatcgcgc tgcacaggcg gcggtgaacg gcggcacggc
     7201 cgacatgatc gggcccatcg accgcccgca cgagttcgtc ttcgtgcccg acgtggggcc
     7261 ggtggttgcg cggctgatcg aaacgccggc agccttcggc aagatctggc acctcgccgg
     7321 cgccggcgtc acgacccagc gcgacctggt ctcggaaatg gagcgccaga ccgggcacaa
     7381 gctcaagctg cgcgtcgccg gcaagacgat gctgcgcctg gtcggcctgt tcaatccttt
     7441 cgtgcgcgag ctggtcgaaa tgaactacct gatgaccgag ccggtaatca tggacgactc
     7501 agctctgcag cggctgatcg ggccggtcca caagacaccc tacgccgagg gcatccgcca
     7561 gacgctggcc gcattgcaga agacgcagtc cttggccgcc gctaccgcgt gagagacatc
     7621 gccgcggccg gccggcttac agcacgggat cgcgcctcac ccgcgaatag accct
//
LOCUS       DASUCY010000610         3379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_142502, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000610 DASUCY010000000
VERSION     DASUCY010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142502"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="VFV25_12105"
     CDS             complement(<1..706)
                     /locus_tag="VFV25_12105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEY1394111.1"
                     /translation="MNALMGHWPWIDPSWVHPVWVRALSWLFIGYFAGLNFVYMALNL
                     VALRLIGRESVYRRLAALPSHSASLEPGIAIIVPAYNEAKTICAAVRSMLQLDYPDFE
                     VIVVNDGSKDETLEVLRREFGMHLFPEAYRISIPVQTVRGVWRSPAYPQLRVLDKANG
                     GRSDAVNAGINAAHHGLVCVVDADSVLQRDSLRRVARPFMEDNRVIASGGTVRISNGC
                     TISQGFIERVAVPKSWL"
     gene            complement(816..1982)
                     /locus_tag="VFV25_12110"
     CDS             complement(816..1982)
                     /locus_tag="VFV25_12110"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEY1394112.1"
                     /translation="MRSTSVFSTTSETDLQLAFTAGAVALMVAFAIAARVMWMRVRDA
                     RRMRELAQVHERWRPLLARAALDGADELSLPPLRKRGELSDVLLLWNQLQEGLRGSAH
                     HNLNRLAQRLGLRDMALRWSQKGDKTDRILGLATLGHLGRAEDAAQVRAALTDRHALV
                     SLAAARALLQIDAKAAVPLVLDEYLSRDNWSAPRVGTLLRDAGADAVGPPLIERLLAA
                     SNDDQLVLLRLLRFAESPRVDSVLHQLLEHSRDPLVLSVALRQLHGPGSLPRVRALAS
                     HKDALVRSAAAVALGRIGTGADRGLLASLMADGDWWVRYRAAQAQLALPGTDATVVIA
                     LRRQLADRYARDILHHVLAEHALAQGFQAGSSATQGDPRTASPQVVPVPQGVRS"
     gene            complement(1948..2769)
                     /locus_tag="VFV25_12115"
     CDS             complement(1948..2769)
                     /locus_tag="VFV25_12115"
                     /inference="COORDINATES: protein motif:HMM:TIGR04390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HEY1394113.1"
                     /translation="MNKHSRCLALVLALAAAGAAADEKPWQVEAGAGAEYLTSNGQQW
                     TQEDLALRSRFAPHSVAELATRHTRRYGQNDDEFGGSVVLPLGTDWLFGASAATSPTH
                     RLLAKDSGSVDLGRRFSGGWLLTGGLSRSLFEIPTGASGGTTFRLNGERYVGDWRFAA
                     GFSHIRLDGGGAADNGGRIQADHYFAERGRIGAVLAVGRELDIEPDLQGVLSIRVESA
                     ALLGVLPLSPDWGLSWSLGSTRNSDAMVRTGPNIGRPVGFTYRRNAVHIGVQHDF"
     gene            complement(2766..3140)
                     /locus_tag="VFV25_12120"
     CDS             complement(2766..3140)
                     /locus_tag="VFV25_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY1394114.1"
                     /translation="MPVVLVIEDDDNIAQLLEFMLEREGYAVKTLADGQVASRHIASE
                     PAPALVLLDSMLPYRDGVALLGEIRARAEWAATPVLMLTAKSLERDIVRALDAGANDY
                     VVKPFQPQELMARVRRLVRETS"
     gene            3255..>3379
                     /locus_tag="VFV25_12125"
     CDS             3255..>3379
                     /locus_tag="VFV25_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEY1394115.1"
                     /translation="MPRKLFVTTALPYANAAFHIGHMMEYIQADIWVRFQRMQGS"
BASE COUNT          570 a         1173 c         1146 g          490 t
ORIGIN      
        1 ccagccagct cttcggcacc gccacccgct cgatgaagcc ctgagagatc gtgcagccgt
       61 tggagatccg aaccgtgccg ccggaggcga tgacgcggtt gtcctccatg aagggccgcg
      121 ccacacggcg caggctgtcg cgctgcagca ccgagtcggc gtcgaccacg cagaccaggc
      181 cgtggtgggc ggcgttgatg cccgcattga cggcatcgga gcgtccgccg ttggccttgt
      241 cgagcacgcg cagctgagga taggcagggc tgcgccacac gcctcgcacg gtctgcaccg
      301 ggatcgaaat gcgataagcc tctggaaaga ggtgcatgcc gaactcgcgc cgcagcactt
      361 ccagggtttc gtccttggag ccgtcgttga cgacgatgac ctcgaagtcc gggtagtcca
      421 gctgcagcat cgagcgcacg gccgcgcaga tggtcttggc ctcgttgtag gccggcacga
      481 tgatggcgat gcccggctcc aggctggcac tgtggctggg gagcgcagcc agccggcggt
      541 acacgctctc gcgtccgatc aggcgcagcg ccaccaggtt gagcgccatg tagacgaagt
      601 tgaggccggc gaagtagccg atgaagagcc aggacagcgc cctgacccat accggatgga
      661 cccaagaggg atcgatccat ggccaatggc ccatgagcgc attcatgacc acccctcgtc
      721 cgacgagtac gtcggccgat cccccgaggg gatgcgggcc ggcttgggag cggcccggcg
      781 cccggcccgc gaagcacagg ttgaaaccgg atgtctcatg atcggactcc ctgcggcacc
      841 ggaacgacct gcggcgacgc ggtgcgtgga tcgccttggg tcgcggatga gccggcctga
      901 aagccctggg ccagggcgtg ctccgccagc acatgatgaa gaatgtcccg cgcatagcgg
      961 tcggccagtt ggcggcgcag cgcgatgacg actgtggcat cggtgccggg cagcgcgagt
     1021 tgcgcctggg ccgcgcgata gcgcacccac cagtcgccat ccgccatcag gctcgccagc
     1081 aggccgcggt cggcgcccgt gccgatgcgg cccagcgcca ccgccgccgc cgagcgcacc
     1141 agggcatcct tgtggctggc cagtgcgcgc acgcgaggca aggagccggg cccgtgcagc
     1201 tggcgcagcg ccaccgacag caccagcgga tcgcggctgt gctccagcag ctggtgcagc
     1261 acgctgtcga cgcgcggcga ctcggcaaag cgcagcagcc tcagcagcac cagctggtcg
     1321 tcgttgctcg cggcgagcag gcgctcgatc agcggcgggc ccaccgcgtc ggctccggcg
     1381 tcgcgcagca gcgtccccac gcgcggggcc gaccagttgt cgcggctcag atattcgtcc
     1441 agcaccagcg gcacggcagc cttggcatcg atttgcagca gcgcccgggc cgccgccaga
     1501 ctgaccagcg catgccgatc agtcaaggcg gctctgacct gggcagcgtc ctcggcacga
     1561 cccaaatgcc ccagcgtcgc cagcccgagg atgcgatcgg tcttgtcgcc cttctgggac
     1621 caacgcagcg ccatgtcgcg caggcccagc cgctgcgcca gccggttcag gttgtggtgc
     1681 gcgctgccgc gcaagccctc ctgcaactgg ttccatagca gcagcacatc ggatagctcg
     1741 ccccgcttgc gaagcggcgg caggctgagc tcgtcggccc cgtccagtgc cgcccgcgcg
     1801 agcaaggggc gccagcgctc gtgcacctgg gccagctccc gcatgcgcct ggcatcgcgc
     1861 acgcgcatcc acatcacccg cgcggcgatc gcaaaagcca ccatcagggc gaccgctccc
     1921 gcggtgaacg ccagttgcag gtcggtttca gaagtcgtgc tgaacaccga tgtggaccgc
     1981 attgcggcgg taagtgaagc ccaccggcct gccgatattc ggtccggtgc gcaccatggc
     2041 gtcgctgttg cgtgtgctgc ccagcgacca gctcagcccc cagtcgggcg agagcggcaa
     2101 gacgccgagc agggcggccg attcgacgcg aattgacagc acgccctgca gatcgggctc
     2161 gatgtcgagc tcgcgcccca ccgccaggac ggcgccgatg cggccgcgct ccgcgaaata
     2221 gtggtcggcc tggatgcgcc cgccgttgtc ggccgcgccg ccgccatcga gacggatgtg
     2281 gctgaagccg gcggcaaagc gccagtctcc gacatagcgc tcgccgttca ggcgaaaggt
     2341 ggtgccgcca ctggcaccag tcggtatctc gaacaggctg cgcgacaggc cacctgtcag
     2401 cagccagccg ccgctgaagc gacggccgag atccacggag cccgagtcct tggccaacaa
     2461 ccgatgcgtc gggctcgtcg cggccgaggc accgaacagc cagtcggtgc cgagcggcaa
     2521 cacgacgctg ccgccgaact cgtcgtcgtt ctgcccgtag cggcgggtgt gccgcgtggc
     2581 gagctcggcg acgctatgcg gcgcaaagcg cgagcgcaag gcgagatcct cctgcgtcca
     2641 ctgctggcca ttgctggtca gatactcggc accggctccg gcttccacct gccacggctt
     2701 ttcgtcggcc gccgcgccgg cagcggcgag cgcaaggact agggcaaggc agcgggaatg
     2761 cttgttcatg aggtctcgcg cacgaggcgc cgcacgcgcg ccatcagttc ctggggctgg
     2821 aaaggcttta cgacatagtc gttggcgccc gcgtccagcg cgcgcacgat gtcacgctcc
     2881 agcgacttgg cggtcagcat cagcaccggc gtggcggccc attccgcgcg ggcgcggatc
     2941 tcgccgagca gggctacgcc gtcgcggtag ggaagcatgg agtcgagcag caccagcgcc
     3001 ggcgccggct cggaggcgat gtggcggctg gccacctgtc cgtcggccag cgtcttcacg
     3061 gcatagccct cccgctcgag catgaactcg aggagctggg caatgttgtc atcgtcctcg
     3121 atgaccagaa cgacgggcat gatgcaggcc gctgggacag cgccggtaca caaagatgtc
     3181 cgcgattcta tggggggtca cacctacaat cgggggcagt ttctcgcctg taccgggcat
     3241 atttaacgca agccatgccg cgcaagctct tcgtcaccac agccctgccc tacgccaacg
     3301 ccgccttcca catcggtcac atgatggagt acatccaggc cgacatctgg gtgcgtttcc
     3361 agcgcatgca gggcagcga
//
LOCUS       DASUCY010000611         4962 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_576160, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000611 DASUCY010000000
VERSION     DASUCY010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4962
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576160"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VFV25_12130"
     CDS             <1..455
                     /locus_tag="VFV25_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003077604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HEY1394116.1"
                     /translation="LFPGNKVRLKYGYVVECTGCEKDADGRITAVLANIVPDTKSGTP
                     GADAVKVKGAITWVGVHDGLQAELRLYDRLFTEAQPDAGGRDFMAALNPGSKQVVTGY
                     LEPSLAKAEADERFQFERHGYFVADRKDHKLGRPVINKIVGLKDNWAK"
     gene            complement(457..1668)
                     /locus_tag="VFV25_12135"
     CDS             complement(457..1668)
                     /locus_tag="VFV25_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEY1394117.1"
                     /translation="MRRRQLLQGGIAITAATLGPSTRLFAAPSGAAAGGAKFLFVFLR
                     GGYDAASLLVPVSSDFYYASRPNIAVPRPSDAANSALPLSSDWGLHPALEDNVALLAS
                     RGQAVFIPFAGTDDLTRSHFETQDSIELGQPLSGHRDYGSGFMNRLAEVLGAGDAIAF
                     TDQLPLCFRGPAQIGNVSLKSVGKPAIDNRTRDLLASMYRNQALAAQVQEGFAVRDEV
                     SREMAGEMEAANRNAITAKGFALEARRIARLMRDKYSLGFVDVGGWDTHVNQGAGTGY
                     LASRFQELGRSLTGFADEMGEDAWRKTVVVVISEFGRTFRENGNRGTDHGHGSVHWVL
                     GGGLSSANDKLAGEQVTVEQRSLFQNRDFPVLNEYRAVLGGLLQRMYGLRAGALERVF
                     PGAQPRDLRLV"
     gene            complement(1696..3282)
                     /locus_tag="VFV25_12140"
     CDS             complement(1696..3282)
                     /locus_tag="VFV25_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019651933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HEY1394118.1"
                     /translation="MTSASRRRWLALAATAPLASSSWLLSGCATTRADTVATVAPSKA
                     RSRAEVYRAVNAMTWGATAKTVDHALQIGYERYVDEQLRAQGGSTLPPTVQAQIDALR
                     IQSTPMATLVTELEAARKSGEAADTEDARKAAQQTFQQDLSRLAREAAARDLLRAVYS
                     NDQLLEHMSWFWFNHFNLHQYKANIRAMLGDYQEHAIRAHALGRFRDLLGAVAHHPAM
                     LIYLDNAQNAVNHINENYARELLELHTLGVDGGYTQRDVQELARVLTGFGVNPSDKQP
                     NVKREWAGLYVRNDLFEFNPNRHDFGDKLVLGRRIKGRGPAELDEVLDLIALHPSTAR
                     FVSRKLALYFLGDDPPQALVARMAGAYTASGGQIAATLRMLLLSSEFTAPRPLAAQKF
                     KDPMHFVVSAVRASYEDKAILNTLPMQNWLNRMGEGLYNHQTPDGYPLTAADWTSSGQ
                     MSTRFEIAKAIGNGSAGLFKTDEANPKEQPAFPQLANALYYETLRGVLGPDTRQALDQ
                     AASPQEWNALLLSSPEFMYR"
     gene            complement(3413..3880)
                     /locus_tag="VFV25_12145"
     CDS             complement(3413..3880)
                     /locus_tag="VFV25_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016721786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1394119.1"
                     /translation="MKSLKTVLIPTLLALAAGVAFAQTAATPAPATNPVQQVDKDNDK
                     IAHDNKDVRKDRRDVRNDKRDIRKDQATLAGDRAQRNADQRRENADIAKGDVKGAEYW
                     NKQRQTENREVANDKRDLRKDSRDLNKDRRDLRADKGERRHDVRKRNHDAGKI"
     gene            4019..4639
                     /locus_tag="VFV25_12150"
     CDS             4019..4639
                     /locus_tag="VFV25_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HEY1394120.1"
                     /translation="MEDARLHSPACERNRQPILDVLQRVLPARGLALEIASGSGQHAA
                     HFAAGLPGWIWQPSEADAQAFASITAWCAGLANVRAPLLLDVTTSPWPVATQFDAIFC
                     ANMLHIAPWSACAGLMRGAAGHLAPQGLLITYGPYLVDGEVPAPSNLAFNADLRARNP
                     AWGLRRLQDVARTAQEAGLQLLQRIAMPANNLVLVFGRRNAAEAAA"
     gene            4651..4959
                     /locus_tag="VFV25_12155"
     CDS             4651..4959
                     /locus_tag="VFV25_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEY1394121.1"
                     /translation="MESRVFTATLQPAKLSFEAPADLPLMQAARRAGIDMPASCRNGS
                     CRTCMCRLLSGRITYRIEWPGLLAEEKAEGYILPCVAHAASDVVIETAGATVIEGRQA
                     "
BASE COUNT          781 a         1636 c         1687 g          858 t
ORIGIN      
        1 gcctcttccc cggcaacaag gtgaggctga agtacggcta tgtggtcgag tgcaccggct
       61 gcgagaagga tgccgacggg cgcatcacgg ccgtgcttgc caatatcgtg cccgacacca
      121 agagcggcac gcccggcgcc gatgcagtca aggtcaaggg tgccatcacc tgggtcggcg
      181 tgcacgacgg cctgcaggcc gagctgcggc tctacgaccg gctcttcacc gaggcccagc
      241 ccgacgccgg cgggcgcgac ttcatggcgg ccctcaaccc cggcagcaag caggtcgtga
      301 ccggctatct ggaaccgtca ttggccaagg ccgaggcgga cgagaggttc cagttcgagc
      361 ggcacggcta tttcgtcgca gatcgcaagg accacaagct ggggcggccg gtgatcaaca
      421 agatcgtggg attgaaggac aactgggcca agtagttcag accagccgca gatcccgcgg
      481 ctgcgcccca ggaaacaccc gctccagcgc ccccgcccgc agcccataca tccgctgcaa
      541 caagccaccc agcacggccc ggtactcgtt gagcaccggg aagtcgcggt tctggaacaa
      601 gctgcgctgc tcgacggtga cctgctcgcc ggccagcttg tcgttggcgc tggacaagcc
      661 gccgcccagc acccagtgca cgctgccatg cccatggtcg gtgccgcggt tgccgttctc
      721 gcggaaggtg cggccgaact cgctgatcac gacgaccacg gttttgcgcc aggcgtcctc
      781 gcccatctcg tcggcgaagc cggtcaggct gcggcccagc tcctggaaac gcgaggcgag
      841 atagccggtg ccggcgcctt ggttcacgtg ggtgtcccag ccgccgacgt cgacgaagcc
      901 caggctgtac ttgtcgcgca tcagcctggc gatgcgacgc gcttccagcg cgaagccctt
      961 ggccgtaatg gcgttgcgat tggcggcctc catctcgccg gccatctcgc gcgagacctc
     1021 gtcgcgcacc gcaaagccct cctgcacctg ggcagccagg gcttggttgc ggtacatgga
     1081 cgccagcaga tcgcgcgtgc ggttgtcgat ggcaggcttg ccgaccgact tcagcgagac
     1141 attgccgatc tgcgccgggc cgcggaagca cagcggcagt tggtcggtga aggcgatcgc
     1201 atcgccggct cccagcactt cggccaggcg attcatgaag cctgagccgt agtcgcgatg
     1261 gccgctcaga ggctggccca gctcgatgga gtcctgggtc tcgaaatggc tgcgcgtcag
     1321 gtcgtcggtg ccggcgaagg gaatgaaaac cgcctggccg cgcgaagcca gcagcgcgac
     1381 gttgtcttcc agcgccggat gcaggcccca gtcgctgctg agcggcagcg cgctgttggc
     1441 cgcatccgag gggcgcggca cggcgatgtt ggggcgcgag gcatagtaga agtcgctgga
     1501 cactggcacc agcaggctgg ccgcgtcata gccgccgcgc aggaagacga acaggaactt
     1561 ggcgccgccc gccgctgcgc cgcttggcgc ggcaaagagc cgcgtgctcg ggcctaacgt
     1621 ggctgcggtg attgcgatgc cgccttggag cagttgtcgt cttcgcatgg cgatgacctc
     1681 ctctagggcc tagccctagc gatacatgaa ctcgggcgag gacagcagca aggcgttcca
     1741 ctcctgaggc gaggcggcct ggtccagcgc ctggcgcgta tcagggccga gcacgccgcg
     1801 cagcgtctcg tagtagagcg cattggcgag ctggggaaaa gccggctgtt ccttgggatt
     1861 ggcctcgtcg gtcttgaaca ggccggcgct gccgttgccg atcgccttgg cgatctcgaa
     1921 gcgggtgctc atctggccgg agctggtcca gtccgccgcc gtcagcggat agccgtctgg
     1981 cgtctggtgg ttgtagagcc cctcgcccat gcggttgagc cagttctgca tcggcagcgt
     2041 gttgaggatg gccttgtcct cgtagctggc gcgcacggcc gagacgacga agtgcatcgg
     2101 atccttgaac ttctgcgcag ccagcggacg cggcgccgtg aactcggacg agagcagcag
     2161 catgcgcaag gtggcggcga tctgcccgcc gctggcggtg taggcgccag ccatgcgggc
     2221 caccagcgct tgcggaggat catcgcccag gaaatacaag gccagcttgc gcgaaacgaa
     2281 gcgcgcggtc gagggatgca gggcgatcag gtccagcacc tcgtccagct cggcaggccc
     2341 gcgccctttg atgcgccgcc cgagcaccag cttgtcgccg aaatcgtggc ggttgggatt
     2401 gaattcgaac aggtcgttgc gaacgtagag ccccgcccat tcgcgcttga cgttcggctg
     2461 cttgtcgctc gggttgacgc cgaagccggt cagcacgcgc gccagctcct gcacatcgcg
     2521 ctgcgtgtag ccgccgtcga cgcccaaggt gtgcagctcc agcagctcgc gggcgtagtt
     2581 ctcgttgatg tgattgaccg cgttctgcgc attgtcgaga tagatcagca tcgccgggtg
     2641 atgcgccacg gcgcccagca gatcgcggaa gcggccgagc gcatgggcgc ggatcgcatg
     2701 ctcctggtag tcgcccagca tcgcgcggat gttggccttg tactggtgca gattgaagtg
     2761 gttgaaccag aaccagctca tgtgttcgag cagctggtcg ttggagtaca cggcgcgcag
     2821 caggtcgcgc gccgccgcct cgcgcgccag gcggctcaag tcctgctgga aggtttgctg
     2881 cgcggccttg cgcgcatcct cggtgtcggc cgcctcgccg ctcttgcgtg ccgcctccag
     2941 ctcggtcacc agcgtggcca tgggcgtgct ttgtatgcgc agcgcgtcga tctgcgcctg
     3001 gaccgtgggc ggcagggtcg agccgccctg ggcgcgcaac tgctcgtcca catagcgctc
     3061 gtagccgatc tgcagcgcgt gatccaccgt cttggcggtg gcgccccagg tcatcgcatt
     3121 gacggcgcgg tagacctcgg cgcgcgatct cgccttggat ggggcgaccg tcgccaccgt
     3181 gtcggcacgc gtcgtggcgc agcccgacag cagccaggaa ctggacgcca aaggcgccgt
     3241 ggcggccagc gcgagccagc ggcggcgcga tgccgatgtc actgccgatg ccagtgccga
     3301 tgcgggctga agctccttct tgttcatcgc cggaagcccc gatggatctg caaaaacaaa
     3361 acaggccgat cgagaaacct tcgatcagcc tgtcgttggc gtcaaccccg gatcagatct
     3421 tgccggcgtc gtgattgcgc ttgcgcacgt cgtggcggcg ctcgcccttg tcggcccgca
     3481 gatcgcggcg atccttgttc agatcgcgcg agtccttgcg cagatcgcgc ttatcgttgg
     3541 cgacctcgcg gttctcggtc tggcgctgct tgttccagta ctcggcgccc ttcacatcgc
     3601 ccttggcgat gtcagcgttc tcgcggcgct gatcggcatt gcgctgggcg cggtcgccgg
     3661 cgagcgtggc ttgatccttg cggatgtcgc gcttgtcatt gcgcacatcg cggcggtcct
     3721 tgcggacgtc cttgttgtcg tgcgcgatct tgtcgttgtc cttgtccacc tgctgcaccg
     3781 gattggtggc aggcgcgggc gtcgcagcgg tctgcgcgaa cgcgacaccg gcagccagcg
     3841 ccagcagcgt ggggatcaac acagtcttga ggctcttcat ataccgtctc ctcggtaata
     3901 gttgctcggt actgccacaa cgccatcccc ttcgagcgag ttgacggccc gaaacgctgc
     3961 gtcagtcttg cgtcagtggc cggagagtac ggtggccccc ttcatccgcc ggacataaat
     4021 ggaagacgcc cgactgcaca gccccgcctg cgagcgcaat cgccagccca tcctcgatgt
     4081 gctgcagcgc gtgctgccgg ctcgcgggct ggcgctggag atcgccagcg gcagcggcca
     4141 gcacgcggcg catttcgcgg ccggcctgcc gggctggatc tggcagccga gcgaggcgga
     4201 tgcacaggcc tttgcatcca tcacggcctg gtgcgccgga ctcgccaatg tgcgtgcacc
     4261 tttgctgctg gacgtgacca cctcaccctg gccagtggcg acgcagttcg atgccatctt
     4321 ctgcgccaac atgctgcaca tcgcgccgtg gtctgcctgt gcaggcttga tgcgcggcgc
     4381 ggccgggcac ttggcgccgc aaggcctgct gatcacttat gggccttatc tggtggacgg
     4441 cgaggtgccc gcgccgagca acctcgcctt caacgccgat ctgcgcgcgc gcaacccggc
     4501 ctggggcctg cgccggctgc aggatgtcgc gcgaacggct caagaagccg ggctgcaatt
     4561 gctgcagcgc atcgcgatgc cggccaacaa tctcgtgctg gtgttcggcc ggcgaaacgc
     4621 ggcggaggct gcggcatgag cgaggcggac atggagagcc gcgtgttcac tgcaacgctg
     4681 cagcctgcca agctgagctt tgaggctccc gccgatctgc ccctgatgca ggccgcacgg
     4741 cgcgccggca tcgacatgcc ggcatcgtgc cgcaacggca gttgccgcac ctgcatgtgc
     4801 cggctgctca gtggccgcat cacctatcgc atcgaatggc cggggctgct ggccgaagag
     4861 aaggcggaag gctacatcct gccctgcgtg gcacacgcgg ccagcgatgt tgtcatcgag
     4921 accgcgggcg ctaccgtcat cgagggcagg caggcctgag gc
//
LOCUS       DASUCY010000612         3468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_608099, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000612 DASUCY010000000
VERSION     DASUCY010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608099"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VFV25_12160"
     CDS             <1..307
                     /locus_tag="VFV25_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005014373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEY1394122.1"
                     /translation="TRKIAAIRSLVEHTASYVRERLPKIYSRELVDVIFEQPYCRIGN
                     LVDKRVAQRQAASRYLKELSALGVLKELQAGKEKLFVHPKLMQLLVRDDNESAPYAT"
     gene            complement(328..2646)
                     /locus_tag="VFV25_12165"
     CDS             complement(328..2646)
                     /locus_tag="VFV25_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEY1394123.1"
                     /translation="MHLRTALRRLRPAAPFLAGLSLFLLLCRAQAAEPATPLPAVAAA
                     ASAPAAPRPVTPPNRPRIGLVLSGGGARGIAHVGVLKVLEREHIPVDVIAGTSMGAII
                     GGLYASGMSAEDIERELLKVNWDGIFASRVARQELSQRRKEEDYEVTPALEIGLRNGE
                     IKVPLGTVSSRGLEMLLRRYTLPVRDVRNFDELPIPFRAVATDMESGEARVLGVGDLA
                     LALRSSMSIPGVFTPTDVNERVLGDGGLVDNLPVDVARAMGADIIIAVNIGTPLAGRE
                     SLDSLLGVTRQMISILTEQNVRRSLALLTPRDLLIAPHLGNLTSADFDKTPEFINAGE
                     LQALAQLNELRRLALSDEGYAQWTAARPHTEPPPAAVLKFVEYEGSQLTNPKRFEAQM
                     ESQPGQPFDAARAERDARRLAGTGDYLRSDYQLVSTPQGDGLVFELEDKPWGPNYFRV
                     GLDMATDFRGRGSFNIKIAHNRHWLDDYGGEWRNNVQIGSEPRWYSEWYHPLNWTTGV
                     SNDWFVSTYGEVSRREFQIYSGNNEVGRYRHTLGRVGVDLGQPWGRWGELRMGLVEQV
                     ISDTPEILASTDPALNTTTSSREGGARLGLVVDQLDYAYFPQAGYRFNLAAVLGRQQP
                     VNSETRDFKSLEGEGLVAGTWGSHTLTLLGRWQQANAPNTLGYGRYTLGGFQQLSGYQ
                     PGQIEGNYLAFGRLSYYARLTPPALTRGFFAGATLEAGNAWGSRSQVSLHDLRYGMSL
                     FLGADTGIGPLYLGLGYAPKGTAGVYLVLGRP"
     gene            complement(2794..>3468)
                     /locus_tag="VFV25_12170"
     CDS             complement(2794..>3468)
                     /locus_tag="VFV25_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HEY1394124.1"
                     /translation="EVGKENTTIIDGAGAAADIEARVKQVRVQIEEATSDYDREKLQE
                     RVAKLAGGVAVIKVGAATEVEMKEKKARVEDALHATRAAVEEGIVAGGGVALLRAKQG
                     VGEIKGDNHDQDAGIKIVLRAIEEPMRIIVANAGDEPSVVVNAVAAGKGNYGYNAQNG
                     QYGDMIEMGILDPTKVTRTALQNAASVAALMLTTEAMVAEAPKEEAPAMPGGMGGMGG
                     MGGMDM"
BASE COUNT          498 a         1169 c         1226 g          575 t
ORIGIN      
        1 gacgcgcaag atcgcggcaa tccgtagcct ggtcgagcac acggcgagct atgtgcgcga
       61 gcgcctgccc aagatctaca gccgcgaact ggtggacgtt atcttcgagc agccctattg
      121 ccgcatcggc aacctggtgg acaagcgggt ggcgcagcgc caggccgcgt cgcgctacct
      181 caaggagtta agcgctctgg gcgtgctgaa ggagctacag gccggcaagg aaaagctctt
      241 cgtccatccc aagctgatgc agttgctggt ccgcgacgac aacgagtctg cgccctacgc
      301 cacctgagca tcctgcagcg tgccggttca aggccgcccc agcaccagat acaccccggc
      361 cgtgcccttg ggcgcgtagc ccagccccag gtagagcggg ccgatgccgg tgtccgcgcc
      421 gaggaagagg ctcatgccgt agcgcaggtc gtgcaggctc acctggctgc ggctgcccca
      481 ggcgttgccg gcctccagcg tggcgccggc gaagaagccg cgcgtcagcg cagggggcgt
      541 gaggcgtgcg tagtagctca ggcggccgaa ggcgaggtag ttgccctcga tctgcccggg
      601 ctggtagccg gacagctgct ggaagccgcc cagcgtgtag cggccgtagc ccagcgtgtt
      661 gggcgcattg gcctgctgcc agcggccgag cagggtcagc gtgtggctgc cccaggtgcc
      721 ggccactagg ccctcgccct ccaggctctt gaagtcgcgc gtctcgctgt tcaccggctg
      781 ctgccggccc agcacggcgg cgaggttgaa gcggtagccg gcctgcggga aataggcgta
      841 gtcgagctgg tcgaccacca ggccgagccg ggcgccgcct tcgcggctgg aggtggtggt
      901 gttcagcgcc gggtcggtgc tggcgaggat ctcgggcgtg tcgctgatca cctgctcgac
      961 caagcccatg cgcaattcgc cccagcggcc ccagggctgg ccgaggtcga cgccgacgcg
     1021 gcccagggta tggcgatagc ggcccacttc gttgttgccg gaatagatct ggaactcgcg
     1081 gcggctcacc tcgccgtagg tggagacgaa ccagtcgttg gagacgccgg tcgtccagtt
     1141 cagggggtgg taccactcgc tgtaccagcg cggctcgctg ccgatctgga cgttgttgcg
     1201 ccattcgccg ccgtagtcgt cgagccagtg gcggttgtgc gcgatcttga tgttgaagct
     1261 gccgcggccg cggaagtcgg tggccatgtc caggccgacg cggaaatagt tgggtcccca
     1321 gggcttgtcc tccagctcga acaccaggcc atcgccctgc ggcgtgctga cgagctggta
     1381 gtccgagcgc aggtagtcgc cggtgccggc caggcggcgc gcgtcgcgct cggcgcgcgc
     1441 ggcgtcgaag ggctggccgg gctggctttc catctgggcc tcgaagcgct tggggttggt
     1501 gagctggctg ccctcgtatt cgacgaactt gagcacggcc gcgggcggcg gctcggtatg
     1561 cgggcgcgcg gcggtccatt gcgcatagcc ctcgtcgctg agcgccaggc gccgcagctc
     1621 gttgagctgg gccagggcct gcagctcgcc ggcgttgatg aactcgggcg tcttgtcgaa
     1681 gtcggccgag gtgaggttgc ccaggtgggg cgcgatcagc aggtcgcgcg gcgtgagcag
     1741 ggccaggctg cgcctgacgt tctgctcggt caggatgctg atcatctggc gcgtcacgcc
     1801 cagcagcgag tccagcgatt cgcgccccgc cagcggcgtg ccgatgttga ccgcgatgat
     1861 gatgtcggcg cccatcgcgc gggccacgtc caccggcagg ttgtcgacca ggccgccgtc
     1921 gcccagcacg cgctcgttca cgtcggtggg cgtgaacacg ccggggatgg acatgctgga
     1981 gcgcagcgcc agcgccaagt cgccaacgcc cagcacgcgc gcctcgccgc tttccatgtc
     2041 ggtggcgacg gcgcggaagg gaatcggcag ctcgtcgaag ttgcgcacgt cgcgcaccgg
     2101 cagcgtgtag cggcgcagca gcatttccag gccgcggctg gaaaccgtgc ccaacggcac
     2161 cttgatttcg ccgttgcgaa ggccgatctc cagcgccgga gtgacctcgt agtcctcctc
     2221 cttgcggcgc tgcgacagct cctggcgcgc cacccggctg gcgaagatgc catcccagtt
     2281 gaccttcagc agctcgcgct cgatgtcctc ggcgctcatg ccgctcgcgt aaaggccgcc
     2341 gatgatggcg cccatcgagg taccggcgat cacgtccacc gggatgtgct cgcgctccag
     2401 caccttgagc acgcccacat gggcgatgcc gcgtgcgccg ccgccggaaa gcaccaggcc
     2461 gatgcgcggg cggttgggtg gcgtgacggg gcgcggggca gccggcgccg aggcggctgc
     2521 ggccaccgcg ggcaaaggcg tggccggctc ggcggcttgt gcccggcaga gcaggaggaa
     2581 cagggagagg ccggcgagga agggcgctgc aggccgcagc cgccgcagag cagtacgaag
     2641 atgcatggcc gcgattctgc ggtctggccg gccgcccgca ggcccagggc attccccgtc
     2701 gcgaagatca gcgcgacgtc aggccgcgta ataaaaagaa agggccgcaa tatgcggccc
     2761 ttgttcgcga gcaagaggct cagcgcgcgg acattacatg tccatgccgc ccatgccgcc
     2821 catgccaccc atgccgccgg gcatggccgg ggcttcttcc ttgggcgcct cggcgaccat
     2881 ggcttcggtg gtgagcatca acgcagcgac cgacgcggcg ttctgcagcg ccgtgcgggt
     2941 caccttggtc gggtccagga tgcccatctc gatcatgtcg ccgtactggc cgttctgggc
     3001 gttgtagccg tagttgccct tgccggccgc cacggcgttc accaccacgc tcggctcgtc
     3061 gcccgcgttg gcgacgatga tgcgcatcgg ctcctcgatc gcacgcagca cgatcttgat
     3121 gccggcgtcc tggtcgtggt tgtcgccctt gatctcgccg acgccttgct tggcgcgcag
     3181 cagcgcgacg ccgccaccgg ccacgatgcc ctcttcgacg gcagcgcgcg tggcgtgcag
     3241 ggcgtcctcg acgcgggcct tcttctcctt catctcgact tcggtggcgg caccgacctt
     3301 gatcaccgcc acgccgccgg ccagcttggc cacgcgctct tgcagcttct cgcggtcgta
     3361 gtcgctggtg gcttcctcga tctgcacgcg cacttgcttg acgcgagcct cgatatcggc
     3421 cgcagcgccg gcgccgtcga tgatggtggt gttttccttg cccacttc
//
LOCUS       DASUCY010000613         2686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_383647, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000613 DASUCY010000000
VERSION     DASUCY010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383647"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VFV25_12175"
     CDS             complement(<1..214)
                     /locus_tag="VFV25_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEY1394125.1"
                     /translation="MSDSDQQHRQFEAAALPHLNAAYNLARWLLRDEHDAEDVVQEAY
                     LRAFRFFAALRGADAKPWLLGIVRNAC"
     gene            352..756
                     /locus_tag="VFV25_12180"
     CDS             352..756
                     /locus_tag="VFV25_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY1394126.1"
                     /translation="MAGVKIIAAAAVLLLQSAASHAEGDAQRGQQLYQQLCAACHSPD
                     YNGVGPAHRGVFGARAATARGYSYSTALAASKLRWDAQNLDRWLAGPEALVPGQKMGV
                     SVPGAQDRADLIAYLATLSVPANAANALRPRE"
     gene            833..1705
                     /locus_tag="VFV25_12185"
     CDS             833..1705
                     /locus_tag="VFV25_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEY1394127.1"
                     /translation="MLDRRRFLELTALGGGAVFASGLPGCATRPPEQDFYFVQLSDAH
                     WGYRGPDNPEAATTLKRAVASVNGLSVPPDFVVFTGDLTHTTDDPGERRRRMGEFRDI
                     VSALKVKDVHFMPGEHDASLDRGAAFQEFFGATHYSFDHKGVHFIALDNVSDPGARIG
                     DEQLAWLKADLATQKEDARIVVLTHRPLFDLAPAWDWATRDGAKAVALLLPYRNVTVF
                     YGHIHQEHHHMTEHIAHHSAKSLIFPLPIPGSQVKRTPIPWDASQPFRGLGFREIEAE
                     AGRYQLTEFPVEKT"
     gene            1716..2084
                     /locus_tag="VFV25_12190"
     CDS             1716..2084
                     /locus_tag="VFV25_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEY1394128.1"
                     /translation="MKDESIERSRLQLLAMLLASAVAPLVRGAEPAEQVIEMTARRFE
                     YTPSEITVKAGVPVLLKIRSIDFVHGFSLPALQLRADLPPGQVTEVRFTAPQVGVLPF
                     LCDNFCGEGHETMNGRIVVT"
     gene            complement(2198..>2686)
                     /locus_tag="VFV25_12195"
     CDS             complement(2198..>2686)
                     /locus_tag="VFV25_12195"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY1394129.1"
                     /translation="EELRLTIDSMDQHGNDLGTLLAMVRYRLGDRLRWAGLDLQWEVG
                     DMPPLPVLAHDGALHAMRIVQEAINNILKHAQASTIRVSTAVDPARSAAVLRICDDGR
                     GFAPSAIDPLHGHGLGSMHRRADKLGATLNLVSRPGETVVELLLPLPQEWVAAGESRT
                     IQ"
BASE COUNT          438 a          917 c          894 g          437 t
ORIGIN      
        1 agcaggcatt gcgcacgatg cccagcagcc agggcttggc atcggcgccg cgcagcgcgg
       61 cgaagaagcg aaaggcgcgc aaataggcct cctgcaccac gtcctcggcg tcgtgctcgt
      121 cgcgcagcag ccagcgcgcc aagttgtagg ccgcgttcag gtgcggcagc gcggcggctt
      181 cgaattgacg gtgttgttga tcgctgtcgc tcaagagaga cgccttgtac agaagacaga
      241 cactggggac atccacttta ttcccgcacc gattcagcgc aatgacaggg aatagagcgg
      301 cagcgtgcgc cgtctagcgt ttcgggttct caagccaaga cggaggtgcc tgtggcagga
      361 gtcaagatca tcgccgccgc tgccgtgctc ctgctgcagt cggcggcatc ccatgccgaa
      421 ggcgacgcgc agcgcggcca gcagctctat caacagctgt gcgcggcctg ccattcgccg
      481 gactacaacg gcgtcggccc ggcgcatcgc ggcgtgttcg gcgcgcgtgc cgccacggcc
      541 cgcggctaca gctattcgac ggcgctggcc gcctccaagc tgcgctggga cgcgcagaac
      601 ctggaccgct ggctggccgg ccccgaggcc ctggtgccgg ggcagaagat gggtgtctcg
      661 gtgccgggcg cccaggaccg cgccgacctg atcgcctatc tggcgacgct gtcggtgccg
      721 gccaatgccg ccaatgcctt gcggcccagg gaataaaccg cgccgcggcc acgtcatgca
      781 gatgcggccc gggcattccg ggttcggttt cacctcgatg caggaggttc atatgctgga
      841 tcgacgacgc tttctggaac tgaccgcgct gggcggcggg gccgtgttcg catcgggact
      901 gccgggctgc gccacgcgcc cgcccgagca ggacttctat ttcgtgcagc tcagcgacgc
      961 gcactggggc taccgcggcc cggacaaccc cgaagctgcc accacgctga agcgcgcggt
     1021 ggcgagcgtc aacggcttga gcgtgccgcc ggacttcgtc gtcttcaccg gcgacctgac
     1081 ccacaccacc gacgaccccg gcgagcggcg tcggcgcatg ggcgaattcc gcgacatcgt
     1141 ctcggcgctc aaggtcaagg atgtgcactt catgccgggc gagcacgatg cctcgctcga
     1201 tcgcggcgca gccttccagg aattcttcgg tgcgacgcat tacagcttcg accacaaggg
     1261 cgtgcatttc atcgcgctgg acaacgtctc cgaccccggc gcgcgcatcg gagacgagca
     1321 actggcctgg ctcaaggccg acctggccac gcagaaggaa gacgcacgca tcgtcgtgct
     1381 gacgcaccgc ccgctgttcg acctcgcgcc ggcctgggac tgggccacgc gcgacggcgc
     1441 caaggccgtg gccttgctgc tgccctatcg caatgtcacg gtgttctacg gccacatcca
     1501 ccaggagcac caccacatga ccgagcacat cgcccaccat tcggcgaaat cgctgatctt
     1561 cccgctgccg atcccgggct cgcaggtgaa gcgcacgccc atcccctggg atgcgagcca
     1621 gcccttccgc ggcctgggct ttcgcgagat cgaggccgag gcggggcgct atcaattgac
     1681 cgaattcccg gtagagaaaa cgtgagggcg ctcgcatgaa ggacgagtcc atcgagcgtt
     1741 cgcgcctgca actgctcgcc atgctgctgg cgagcgccgt ggccccgctg gtgcgcggcg
     1801 ccgagccggc ggagcaggtg atcgaaatga ccgcgcggcg cttcgagtac acgcccagcg
     1861 agatcacggt caaggcgggc gtgccggtgc tgctgaagat ccgctcgatc gacttcgtgc
     1921 atggcttctc gctgcccgcg ctgcagctgc gcgccgacct gccgcccggt caggtgaccg
     1981 aagtgcgttt caccgcgccg caggtcggcg tgctgccctt cctgtgcgac aacttctgcg
     2041 gcgagggcca cgaaaccatg aacgggcgca tcgtggtgac ctgagtgcgc ggctgccacg
     2101 ctcggcgctg gcggaagagg tttgcagctg gtctagtcca gctgctacga aaattgcagc
     2161 gaaatttgtt aaaaatacac cagtattctg gtgattttta ttgaatggtg cgcgactcgc
     2221 ccgccgccac ccattcctgc ggcagcggca gcagcagctc caccaccgtc tcgcccggcc
     2281 gcgataccag gttcagcgtt gcgcccagct tgtcggcgcg gcggtgcatg ctgcccaggc
     2341 catggccatg cagcgggtcg atcgccgacg gcgcgaagcc gcgcccgtca tcgcagatgc
     2401 gcagcacggc ggcgctgcgc gcagggtcca ccgcggtgct gacgcggatc gtgctggcct
     2461 gcgcgtgctt gaggatgttg ttgatcgcct cctgcacgat gcgcatcgca tgcagcgcgc
     2521 cgtcgtgggc caggaccggc agcggcggca tgtcgcccac ctcccattgc aggtccaggc
     2581 cggcccagcg caggcggtcg cccaggcggt agcgcaccat cgcgagcagc gtgccgaggt
     2641 cgttgccgtg ctggtccatc gagtcgatgg tcaggcgcag ctcttc
//
LOCUS       DASUCY010000614         3916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1476006, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000614 DASUCY010000000
VERSION     DASUCY010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1476006"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(333..1307)
                     /locus_tag="VFV25_12200"
     CDS             complement(333..1307)
                     /locus_tag="VFV25_12200"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEY1394130.1"
                     /translation="MNRRDLGRAWGAWGKKSLTVACALATALLASCGGGGSSVNPFYP
                     DHIIAFGDESSVINSDGSKYTINALNTSNQLDCASNPIWVQYLAANFGIVFSQCNPNA
                     DPNPKGVMFAKVGAKVADVVSQVQQYQAGNSIGSKDLFTILVGQNDILSAFLLYGAQS
                     ETDLIAAVEAQGVALGQLINSIATAGGKVIVVTVPDMGLTPYAIVQESRNPGSAAVLS
                     HLTDRFNVAMRTTIINDGSMIGLVLGDELTQSIAQFPQNFGYTNVTLAACSVALPACT
                     TSTLITDPSNGNPATGTTFLWASDRVLGPGAQLRIGQSAVLRARNNPF"
     gene            complement(1354..2358)
                     /locus_tag="VFV25_12205"
     CDS             complement(1354..2358)
                     /locus_tag="VFV25_12205"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEY1394131.1"
                     /translation="MARLLDGHAPAALELAAQRLAEGELVAFPTETVYGLGARADDDA
                     AVAKIFAAKGRPSDHPLIVHVRSQQEAETFAAEITPPARRLMAAFWPGPLTVILPRRA
                     DMAAAAAGRQDSIGLRCPDHAVARALLDAAARHGVTGVAAPSANRFGRISPTRAAHVL
                     EEFEADDGVWVLDGGACPVGIESSIVDCTRGDPVLLRPGRLTSAEIEAAAGAPLHARD
                     AAAPRASGTLAAHYAPRARLRLMSTEQLRGALQLLADTVEKQKPPTLAVYSRSLRPVP
                     GVVHRTMPQRAAAAAQELFAALRELDASGVQLIWVEEPPASSEWDGVRDRLQRAAAAS
                     "
     gene            complement(2363..3547)
                     /locus_tag="VFV25_12210"
     CDS             complement(2363..3547)
                     /locus_tag="VFV25_12210"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEY1394132.1"
                     /translation="MTAVILPGVLDALGRPATLGVVGGGQLGRMFVHAAQGLGYETAV
                     LDPDPQSPAGLVAHHHIHADYLDERGLAELARLSEAITTEFENVPARALEALAQHRPV
                     APGAAAVAVCQDRAAEKAHFQRCGVPCAPHALIETAEDLALVNQALLPGILKTARLGY
                     DGKGQRRVSSRAELVAAWEALGSVPCVLEQMLPVAFEVSVIVARNADGAIVHFPLQQN
                     LHRDGILAVTSVPAPDVTPALQEQACAAAETIAASMGYVGVLCVEFFVLKDGGLVANE
                     MAPRPHNSGHYTIDACDVSQFELQVRAMAGLPLVAPRLHSPAVMLNLLGDLWFNAQGA
                     VRVPPWDRVLALPGTHLHLYGKAAARPGRKMGHLTLTAASPEAARDAALQAAELLGIA
                     PF"
     gene            complement(3544..>3916)
                     /locus_tag="VFV25_12215"
     CDS             complement(3544..>3916)
                     /locus_tag="VFV25_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Kdo hydroxylase family protein"
                     /protein_id="HEY1394133.1"
                     /translation="VGESFEGVARRFLPRVGRYRPWLAGLLYRLGITKRRRTHYDHLM
                     LGLHDAMKADADYQQNGVQTTVEFAAGTAWVCYSDQTPHAAMSGQHMLEQTLFVALSA
                     LSHPELSPLAVLERLSGWILL"
BASE COUNT          629 a         1320 c         1340 g          627 t
ORIGIN      
        1 acgcagcgga tttctgggct gaaaggcggg tcgcattcag gtgcttctat tcttttcagt
       61 agcccgttcc caggacaatg gccagtggga acgggcgacg gaaacggccc tgacgaacaa
      121 tgaacaacag ccagccaaga cggcagccaa gagtgccaag cactcggcgc ctagcttcgg
      181 tgcgcacatg ccgctgatga gcagcgcctt cgacacggcg atgtggttgg ccgcagccta
      241 agcgctgcgt gtcgggaaaa gaaacgggcg acttcggtcg cccgtttttt gcttttggga
      301 gagctaaaca ctcaccccgt ctaagaacca gcctagaagg ggttgttcct cgctcgtagc
      361 accgcagact ggccgatgcg cagttgcgcg cccgggccca gcacgcggtc gctggcccac
      421 aggaaggtgg tgccggtggc agggttgcca ttgctggggt cggtgatgag ggtggaagtc
      481 gtgcaggcgg gcagcgccac gctgcaggcc gccagcgtca cattggtgta gccgaagttc
      541 tgcgggaact gggcgatgga ttgggtcagc tcatcgccca gcaccaggcc gatcatgctg
      601 ccgtcgttga tgatggtggt gcgcatcgcg acattgaagc ggtcggtgag gtgcgacagc
      661 acggccgcgc ttcctgggtt gcggctctcc tggacgatcg cgtaaggcgt caggcccatg
      721 tcgggcacgg tcacgacgat gaccttgccg ccggccgtcg cgatcgagtt gatcagctgc
      781 cccagtgcca cgccttgggc ctccacggcg gcgatcaggt cagtctcgct ttgcgctcca
      841 tagagcagga aggcggacag gatgtcgttc tggccgacca ggatagtgaa tagatccttg
      901 gagcctatgc tgttgccggc ctggtactgc tggacctgcg acacgacatc ggctaccttg
      961 gcgccgacct tggcgaacat cacgcccttg gggttggggt ccgcattcgg gttgcactgc
     1021 gaaaacacga taccgaagtt cgccgcgagg tactgcaccc agatcgggtt gctggcgcaa
     1081 tccagctggt tgctcgtgtt cagcgcattg atcgtgtact tgctgccatc gctattgatc
     1141 acgctgcttt cgtcgccaaa agcgatgatg tgatcaggat agaaggggtt cacgctgctg
     1201 ccgccgcctc cacaggaggc aagcaaggcg gttgccagcg cgcaggcgac ggtcagcgac
     1261 ttcttgcccc atgcgcccca tgcgcgccct agatcacgac gattcattca atctccactc
     1321 ttgcgattcg gtcgggtgcc agatgccgct ctctcagctg gcggcggccg cgcgctgcag
     1381 cctgtcccgc actccgtccc attccgacga tgcgggcggc tcctcgaccc agatgagctg
     1441 cacgcccgaa gcatcgagct cgcgcagcgc agcgaacagt tcctgggcgg ctgccgccgc
     1501 ccgctgcggc atggtcctgt gcacgacacc cggcacgggc ctcaagctgc gggaatatac
     1561 agccaaggtc ggcggctttt gtttctccac ggtgtccgcc agcaattgaa gcgcgccgcg
     1621 cagctgctcg gtgctcatca gtctgagcct ggcacgcggc gcgtagtgcg cggccagagt
     1681 gccggacgcg cgcggcgcgg cggcatcgcg tgcgtgaagc ggcgcgcccg cggcagcctc
     1741 gatttcggca ctggtcagcc gccccgggcg cagcagcacc gggtcgccgc gcgtgcagtc
     1801 gacgatgctc gattcgatgc ccaccgggca ggcgccgccg tcgagcaccc agaccccgtc
     1861 gtcggcttcg aactcctcca gcacatgggc ggcccgcgtc gggctgatgc ggccgaagcg
     1921 gttggcgctg ggcgctgcca cgcccgtcac gccgtgcctg gccgccgcat cgagcagcgc
     1981 gcgcgccacc gcgtggtccg ggcagcgcag gccgatgctg tcctgccggc ctgcagccgc
     2041 tgcggccatg tcggcgcggc gcggcaggat cacggtcaat ggcccgggcc agaaagccgc
     2101 catcagccgg cgagccggcg gcgtgatctc agccgcgaag gtctcggcct cctgctgcga
     2161 gcgtacatgg acgatcagcg ggtggtcgct gggccggcct ttggcggcga agatctttgc
     2221 aaccgccgca tcgtcgtcgg cccgcgcgcc caggccatag acagtctcgg tcgggaaggc
     2281 gaccagctcg ccctcggcca gccgctgcgc ggctagctcc agggcggcgg gcgcatgtcc
     2341 gtcgagcaat cgggccatcg ctctagaacg gtgcgatgcc gagcagttcc gctgcctgca
     2401 gcgcggcatc acgcgccgcc tcggggctcg cggcggtcag cgtcaggtgc cccatcttgc
     2461 gtcccggtcg ggcagcggcc ttgccataca gatgcagatg ggtacccggc agcgcaagca
     2521 cacggtccca gggcggcacg cgtaccgcgc cttgggcatt gaaccagaga tcgcccagca
     2581 gattcagcat cacggccggc gaatgcaggc gcggtgcgac cagcggcagc cccgccatcg
     2641 cgcgcacctg cagctcgaac tgggacacat cgcaggcgtc gatcgtgtaa tggcccgagt
     2701 tgtgcgggcg cggcgccatc tcgttggcga cgaggccgcc gtccttcagc acgaagaact
     2761 ccacgcacag cacgcccaca tagcccatgg acgccgcgat cgtctcggcc gcagcgcaag
     2821 cctgctcctg caaggcgggc gttacatcgg gcgccggcac gctggtgacg gccaggatgc
     2881 cgtcgcgatg caggttctgc tgcagcggga agtgcacgat cgcgccgtcc gcattgcgcg
     2941 cgacgatgac gctgacctcg aaggccaccg gcagcatctg ctccagcacg cagggcacgc
     3001 tcccaagcgc ctcccaggcc gcgaccagct cggcgcgact gctcacgcgc cgctgcccct
     3061 tgccgtcata gcccaggcgc gcggtcttga ggatgccggg cagcagggcc tggttgacca
     3121 gggccaggtc ctcggccgtc tcgatcaagg cgtgcggcgc gcaaggcacg ccgcagcgct
     3181 ggaaatgcgc cttctcggcg gcgcggtcct ggcagaccgc gaccgccgcc gcgccgggcg
     3241 ccaccggccg gtgctgggcc aaggcttcca gcgcgcgggc cggcacgttc tcgaactcgg
     3301 tcgtgatggc ctcgctcagg cgcgccagct cggccaggcc gcgctcgtcc aggtagtcgg
     3361 catggatgtg atggtgcgcg accaggccgg ccgggctctg cgggtcgggg tcgagcacgg
     3421 ccgtctcgta gcccaggccc tgggccgcgt gcacgaacat gcgcccgagc tgaccgccgc
     3481 caaccacacc cagcgtggcg ggccggccga gcgcatcgag gacgccgggg aggatgacgg
     3541 cagtcatagc aggatccaac cgctcaggcg ttcgagcacg gccagcggcg agagttcggg
     3601 atgcgacagt gccgacagcg cgacgaacag cgtctgctcc agcatgtgct gaccgctcat
     3661 cgcggcgtgc ggagtctggt cgctgtagca gacccaagcc gtgccggcgg cgaattccac
     3721 cgtcgtctgc acgccatttt gctggtagtc ggcatcggcc ttcatcgcat cgtgcagtcc
     3781 gagcatcagg tgatcgtaat gggtgcggcg gcgcttggtg ataccaaggc gatacagcag
     3841 gccggccagc catgggcgat agcgccccac gcgcggcagg aagcgccttg ccacgccttc
     3901 gaaggattcg ccgacc
//
LOCUS       DASUCY010000615         2790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_704937, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000615 DASUCY010000000
VERSION     DASUCY010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704937"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..126
                     /locus_tag="VFV25_12220"
     CDS             1..126
                     /locus_tag="VFV25_12220"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1394134.1"
                     /translation="MGQKFRITGTPAIFFEDGSRVPGAMGAADVEKQFEAVGKKS"
     gene            complement(146..946)
                     /locus_tag="VFV25_12225"
                     /pseudo
     CDS             complement(146..946)
                     /locus_tag="VFV25_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167478.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
     gene            complement(995..2071)
                     /locus_tag="VFV25_12230"
     CDS             complement(995..2071)
                     /locus_tag="VFV25_12230"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEY1394135.1"
                     /translation="MMGRRSIALALLALAVLLVHGLLLRRVGDQLASIRDIHPGPPHI
                     KVNFVRKLALAAPPRAKPTPPQPAPRREARHTVHGPAPGASRPASQPEEAVSAPEPVT
                     SIASASAASEAASGAIAAAASASAVEAAASSPGELFRWPPSTRLSYTLNGYYRGEVQG
                     TAQVQWLHEEDHYQVLIDIYIGPRFAPLMSRHMSSDGLITPDGLAPRRYDQDTRVGFS
                     SRRTSMELGADLVKLANGSRAWRPVGMQDSASQFVQLTYEFTVRPEGLRAGNRIAMPL
                     ALPRQFSVWIYEVVGEEMLRTPFGEVSAFHVKPLREPGGGDLVAEAWFAPQLQYLPVR
                     FHIVQDAQSYVDLMIQRLPEQAAR"
     gene            complement(2085..>2790)
                     /locus_tag="VFV25_12235"
     CDS             complement(2085..>2790)
                     /locus_tag="VFV25_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase M61"
                     /protein_id="HEY1394136.1"
                     /translation="TPGRAVQSVAQSSFDTWTKYYRPDENTPNATVSYYTKGALVAMA
                     LDLTLRREGKGNLDAVMQRLWTQSGGGPITEADIAAALQAVGKRSYAKELAAWVHGKG
                     ELPLAELLGELGVSWTQDKPTLGQRLGARTAESNGSLKVQAALRGGAAEQAGLAAGDE
                     LIALDGWRLKKLDDLGALRAFEQPLPLLAARDGRLLQLTLPTTTGSAQGTVSLASDEK
                     AKRAVQQRRASWLACG"
BASE COUNT          383 a          927 c         1013 g          467 t
ORIGIN      
        1 ctgggccaga agttccgcat cactggcacg ccggccatct tcttcgagga cggcagccgc
       61 gtgccgggcg cgatgggcgc ggcggatgtg gagaagcagt ttgaggccgt cggtaaaaag
      121 tcctgaactg ccctgccgag cctgatcatg agtgtttgaa gtcgggcttg cgcttgttca
      181 ggaaggcgtc catgccttcc ttctggtcct gggtcgcgaa cagcgagtgg aagacgcggc
      241 gctcgaacag caggccttag ttgagcggtg attcgtaggc gcggttcacg cattccttgg
      301 cggccatcag gctgaccagc gagaactcgc acatcgcgcc ggccgcggcg gcggcctcct
      361 cgagcagctt gtcggccggc accacgcgtg acacgaggcc ggcgcgctcg gcctcggccg
      421 cgtccatcat gcgggcggtg agcgccagat ccatggcctt ggacttgccg accgcgcgcg
      481 gcaggcgctg cgtgccgccg gcgccgggga tgatgccgat cttgatttcg ggctggccga
      541 accgggcgct gtcggccgcg atgatgaggt cgcacatcat cgccagctcg cagccgccgc
      601 ccagggcata gccggctacc gcggcgatca ccggcttgcg cacgcgcttc atggtctccc
      661 agttgcgcgt gatgaaatcg gtcttgtaga cgtccatgaa gccgcgcggt gccatcgcgg
      721 cgatgtcggc gccggccgcg aaggccttgt cgctgccggt gatcacgatg cagccaatgc
      781 cgtcgtcggc gtcgaaggcg agcagcgcgg cgcccagctc gtccatcagg ccgtcgttca
      841 gcgcattgag ctgcttgacg cggttgagcg tgatccagcc ggcgcgcagg gcgttggcgg
      901 cttgcgagac gctgcgggtt tcggtgagga tgtattcgta ggccatggcg ggtgtctcct
      961 tgcagcctgc actctaaccg cgctgactcg aggctcatcg agcggcctgc tccggcagcc
     1021 gctggatcat caggtcgacg tagctctgcg cgtcctgcac gatgtggaag cgcaccggca
     1081 ggtattgcaa ttgaggcgcg aaccaggcct cggcaaccag gtcgccgccg cccggctcgc
     1141 gcagcggctt cacgtggaag gccgagacct cgccgaaggg cgtgcgcagc atctcctcgc
     1201 cgacgacctc gtagatccag acgctgaact ggcgcggcag cgcaagcggc atcgcgatgc
     1261 ggttgccggc gcgcaggccc tccgggcgca cggtgaactc gtaggtcagc tgcacgaact
     1321 ggctggccga gtcctgcatg ccgacggggc gccaggcgcg gctgccgttg gccagcttca
     1381 ccaggtcggc gccgagctcc atgctggtgc ggcggctgct gaagccgacg cgcgtgtcct
     1441 ggtcgtagcg gcgcggtgcc aggccatcgg gcgtgatgag gccgtcgctg ctcatgtggc
     1501 gcgacatcag cggcgcgaag cgcgggccga tgtagatgtc gatcagcacc tggtaatggt
     1561 cttcctcgtg cagccattgg acctgggccg tgccctgcac ctcgccgcgg tagtagccgt
     1621 tgagcgtgta gctcagacgc gtggagggtg gccagcggaa gagctcgccg ggtgagctgg
     1681 cggctgcctc cacggcgctg gcggacgccg cggcagcgat ggcgccggat gccgcctcag
     1741 acgcagcaga ggcagaggcg atgctcgtta ccggctcggg cgctgagacg gcttcttcag
     1801 gctgagaggc cggccgcgaa gcccctggcg cggggccgtg cacggtatgc ctggcctcgc
     1861 gacgcggcgc aggctgggga ggtgtaggtt tggcccgcgg tggagctgcc agggccaact
     1921 tgcgtacgaa attgaccttg atgtgcggcg gcccggggtg gatgtccctg atgctggcga
     1981 gctggtcgcc aacgcgcctg agcagcagcc cgtggaccag cagcacggcc agcgccagca
     2041 gcgcaagcgc gatgctgcgg cggcccatca tcaccggcgt ggcttcagcc gcaggccagc
     2101 cagctcgcgc gccgctgctg gacggcccgc ttggccttct cgtcgctcgc aaggctgacc
     2161 gtaccctggg cgctgccggt ggtggtgggc agggtgagct gcagtaggcg gccgtcgcgc
     2221 gcggccagta gcggcagcgg ctgctcgaag gcgcgcaaag cgccgaggtc gtcgagtttc
     2281 ttgagccgcc agccgtccag cgcgatcagc tcgtcgcccg cggccagccc ggcctgctcg
     2341 gctgcgccgc cgcgcagcgc ggcctgcacc ttaaggctgc cgttgctttc cgcggtgcgc
     2401 gcgcccaggc gctgcccgag cgtgggcttg tcctgggtcc agctcacgcc cagctcgccc
     2461 agcagctcgg ccagcggcag ctcgcccttg ccgtgcaccc aggcggccag ctccttggcg
     2521 tagctgcgct tgcctacggc ctgcagtgcg gcggcaatgt cggcctcggt gatggggccg
     2581 ccgccgctct gcgtccacag gcgctgcatc acggcgtcca ggttgccctt gccttcgcgg
     2641 cgcagtgtca ggtccagcgc catggccacc aacgcgccct tggtgtagta gctcaccgtg
     2701 gcgttaggcg tgttctcgtc cgggcggtag tacttggtcc aggtgtcgaa gctggactgg
     2761 gccacactct gcaccgctcg gcccggtgtg
//
LOCUS       DASUCY010000616         3750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_993734, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000616 DASUCY010000000
VERSION     DASUCY010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993734"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="VFV25_12240"
     CDS             complement(<1..734)
                     /locus_tag="VFV25_12240"
                     /inference="COORDINATES: protein motif:HMM:NF014919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HEY1394137.1"
                     /translation="MTGAISEADVEALLGARHADPFGVLGLHAAEDGRLWLRALLPGA
                     RAVWAVDAPSGQRLAPLAVRHPDGLFEGLIPRRRKHFDYRLDVLWDDGREERLADAYA
                     YGTQLAEADLQALRAGEHLRPYTVLGAHPLRHNGVAGVRFAVWAPNASRVSVVGDFNR
                     WDGRRHAMRLRHEAGVWEIFVPGAALGHLYKFELLDAAGQLLPLKADPYARAAQLRPE
                     TASRVAGLPPVRALSPQRGLANVRSAA"
     gene            complement(756..3203)
                     /locus_tag="VFV25_12245"
     CDS             complement(756..3203)
                     /locus_tag="VFV25_12245"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HEY1394138.1"
                     /translation="MPNLMTFSPETTAARDPRTAFRAAFARELGQCSEGVGSEACMRA
                     AAQACRELLAERWARTQAEDAKSTGSSRRVHYLSMEFLMGRALHNALTALGLEADVRQ
                     QLQALGQQLPDVLEHEHDAALGNGGLGRLAACFLDSFAELGLPSFGYGLRYQYGMFAQ
                     AIQQGRQVEAADDWMRLGNAWETPRPDVRFSVGFGGRVEGDGGGRRWVPAERLVAQAY
                     DFIVPAHHSERVSTLRQWHASAQEPIDFAAFCRGDYAAASSHRVAADALNWVLYPDDS
                     TEAGRELRLKQEAFLVSASLQDLIARHLREQGSLQTLGRRNAIHLNDTHPALAPAELM
                     RLLLDEHGLPWDEAWAITREAVSYTNHTLMSEALETWALRMFEALLPRHLEIIYEINH
                     RFLEEVRRRFPGDDAMLSRVSLIDETGERRVRMAALAIVASHKVNGVSALHSDLMVQT
                     IFADYAKLYPERFLNVTNGVTPRRWLMQANPALSRLIDDSIGRGWRRDLDRLAELKPL
                     ADDPALGGEFLAAKRQNKERLAALIRRELGIAVDPGSLFDVQIKRVHEYKRQLLNILQ
                     VVARYQAIIDKPGAAWAPRTVVIAGKAASAYQTAKLIIQLAHDVARVVNIDPRTEGRL
                     KLVFLPNYGVSLAETILPAADLSEQISTAGTEASGTGNMKFALNGALTIGTWDGANIE
                     MAQAMGEENMFIFGLRTQAVRQMKALGYDPRLYVEQDLQLRRVIDAVAGGEFSPGDPQ
                     RYRGLIDNLLSRDTYMLMADFADYVATQGRVDALFATPTAWVRRALLNVAAMGSFSSD
                     RTIREYVDRVWSATAPR"
     gene            3533..>3750
                     /locus_tag="VFV25_12250"
     CDS             3533..>3750
                     /locus_tag="VFV25_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY1394139.1"
                     /translation="MADVKLRAVSKAYGETSILKGIDLEIRDGEFMVFVGPSGCGKST
                     LLRVIAGLEDITSGELTIAGRVVNEVPPA"
BASE COUNT          589 a         1257 c         1312 g          592 t
ORIGIN      
        1 gccgctgagc ggacattggc cagcccgcgc tgcgggctca gcgcccgcac gggcggcagc
       61 ccggccacgc ggctggcggt ctcgggccgc agctgggccg ccctggcgta gggatcggcc
      121 ttgagcggca gcagctggcc ggccgcgtcg agcagctcga acttgtagag atggccgagc
      181 gcggcgccgg ggacgaagat ctcccagacc cccgcttcgt gccgcaggcg catcgcatgg
      241 cgccggccgt cccagcgatt gaagtcgccg accacgctga cccggctggc attcggcgcc
      301 cagaccgcga agcgcacgcc ggcaacgccg ttgtgccgca gcggatgcgc gcccagcacg
      361 gtgtaggggc gcaaatgctc gcccgccctc aaagcctgta gatcggcctc ggcgagctgg
      421 gtgccgtagg cataggcgtc ggccaggcgc tcctcgcggc cgtcgtccca gagcacgtcg
      481 agccggtagt cgaagtgctt gcgtcgccgc ggaatcaggc cttcgaacag accatccggg
      541 tggcgcaccg cgaggggcgc aaggcgctgc ccgctgggcg catccaccgc ccagacggcg
      601 cgggccccgg gcagcagcgc cctcagccag aggcgaccgt cctctgcggc atgcaggccc
      661 agcacgccga aaggatcggc gtggcgggcg ccgagcaggg cctcgacatc ggcctcggag
      721 atcgctccag tcaccgggcg caggcctctt ggttctcagc gcggagcagt ggccgaccag
      781 accctgtcga catactcacg tatcgtgcga tcggaagaga agctccccat cgccgccacg
      841 ttcagcagcg cgcgccgcac ccaggcggtg ggcgtagcga acagcgcgtc gacgcggcct
      901 tgcgtggcca catagtcggc gaagtcggcc atcagcatgt aggtgtcgcg gctcagcaga
      961 ttgtcgatca ggcctcgata gcgctgggga tcgccgggcg agaactcgcc gccggcgacg
     1021 gcgtcgatga cgcgcctgag ctgcaggtcc tgctccacat agaggcgcgg gtcgtagccc
     1081 agcgccttca tctgccgcac ggcctgggtg cgcaggccga agatgaacat gttctcctcg
     1141 cccatcgcct gcgccatctc gatgttggcg ccgtcccagg tgcctatcgt cagcgcgccg
     1201 ttgagggcga acttcatgtt gccggtgccc gaggcctcgg tgccggcggt ggagatctgc
     1261 tcgctcaggt cggcggcggg caggatggtc tcggccaggc tcacgccgta gttgggcagg
     1321 aagaccagct tcaggcgccc ttcggtgcgc gggtcgatgt tgaccacgcg cgccacgtcg
     1381 tgcgcgagct ggatgatgag cttggccgtc tggtaggccg aggctgcctt gccggcgatc
     1441 acgacggtgc gcggcgccca ggcggctccc ggcttgtcga tgatggcctg gtagcgcgcc
     1501 accacctgca ggatgttgag cagctggcgc ttgtactcgt gcacgcgctt gatctgcacg
     1561 tcgaacaggc tgcccggatc gaccgcgatg cccagctcgc ggcggatcag cgccgccagg
     1621 cgctccttgt tctgccgctt ggcggcgagg aattcgccgc ccagcgcggg atcgtccgcc
     1681 agcggcttga gctcggccag ccggtccagg tcgcggcgcc agccgcggcc gatgctgtcg
     1741 tcgatgagcc tggacagcgc ggggttggcc tgcatcagcc agcgccgcgg cgtcacgcca
     1801 ttggtgacgt tgaggaagcg ctcgggataa agcttggcgt agtccgcgaa gatggtctgc
     1861 accatcaggt ccgaatgcag cgccgacacg ccattgacct tgtgcgaggc gacgatggcc
     1921 agcgcggcca tgcgcacgcg ccgctcgccg gtctcgtcga tcagcgacac gcgcgacagc
     1981 attgcgtcgt cgccggggaa gcggcgcctc acctcttcca gaaagcggtg gttgatctcg
     2041 tagatgattt ccaggtgccg cggcagtagc gcctcgaaca tgcgcaaggc ccaagtctcc
     2101 agcgcctcgg acatcagcgt gtggttggtg taggacaccg cctcgcgcgt gatcgcccag
     2161 gcttcgtccc agggcaggcc atgctcgtcc agcagcagac gcatcagctc ggccggggcc
     2221 agcgcagggt gcgtgtcgtt caggtggatc gcgttgcggc ggcccagcgt ctgcaggctg
     2281 ccttgctcgc gcaggtggcg ggcgatcaga tcctgcagcg aggcgctgac caggaaggcc
     2341 tcctgcttca ggcgcagctc gcggccggct tcggtgctgt cgtcgggata gagcacccag
     2401 ttcagcgcgt cggccgcaac gcggtggctg gaagcggcgg catagtcgcc gcggcagaag
     2461 gcggcgaagt cgatcggctc ctgcgccgag gcatgccatt ggcgcagcgt ggacacgcgc
     2521 tcgctgtggt gcgccggcac gatgaagtca taggcctggg ccaccaggcg ctcggccggc
     2581 acccagcgcc gcccgccgcc gtcgccctcc acgcggccgc cgaagccgac cgagaagcgc
     2641 acatcgggac gcggcgtttc ccaggcattg cccaggcgca tccagtcgtc ggccgcctcg
     2701 acttggcgcc cctgctggat cgcttgcgcg aacatgccgt attggtagcg caggccgtag
     2761 ccgaaggaag gcagtcccag ctcggcaaag gaatccagga agcaggcggc caggcggccc
     2821 aggccgccgt tgcccagggc cgcgtcgtgc tcgtgctcca gcacatcggg cagctgctgg
     2881 cccagcgcct gcagctgctg gcgcacatcg gcttccaggc ccagcgcggt cagcgcgttg
     2941 tgcagcgcgc ggcccatcag gaattccatc gacaggtagt gcacgcgacg cgagctgccg
     3001 gtcgacttgg cgtcctcggc ctgggtgcgg gcccagcgtt cggcgagcag ttcgcggcag
     3061 gcctgcgccg cggcgcgcat gcaggcctcg ctcccgaccc cctcgctaca ctggcccagc
     3121 tcccttgcaa aggcggcgcg gaaggcggtg cgcggatctc tcgcggcggt ggtttcgggc
     3181 gagaaagtca tgagatttgg catggtgtga cgttgtaggg acgcgctgcg atgcatcgcc
     3241 ggtccgcgtc caggcaaccg gctggcgcaa tgctagtgcg agacgatgcc caagtgaagc
     3301 agaaatttgt agtttgacta cacaaccgaa cgcacaactt tatctaaccc attgattttc
     3361 aatggtgttg tcgctcaatc taagtgatat cccttgttga gcgcattcct attgtttgta
     3421 tattgtctac attcgccaac ctgatggcgc agcctgtgcg cggcgcgtca gccggcaacc
     3481 atcacctaca aacgagacaa gccggcctga tcgggcggcg cggaggtaag acgtggcaga
     3541 cgtgaagctg cgcgcggtca gcaaggccta cggcgagacc agcatcctca agggcatcga
     3601 cctcgaaata cgcgacggcg agttcatggt cttcgtcggg ccctcgggct gcggcaagtc
     3661 cacgctgctg cgcgtgatcg ccgggctgga ggacatcacc agcggcgagc tgaccattgc
     3721 cgggcgcgtg gtcaacgagg tgccgccggc
//
LOCUS       DASUCY010000617         2302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_447768, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000617 DASUCY010000000
VERSION     DASUCY010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447768"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VFV25_12255"
     CDS             complement(<1..483)
                     /locus_tag="VFV25_12255"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY1394140.1"
                     /translation="MNLDDRPPQLDDVARLLAEPKRLRMLWALGDGRAYTATELAQVA
                     ELAPSAASNHLARLVDAGVVALRVQGRHRYFALQREDVARAVEALGAINLQQPDASLP
                     QAGVPRRLPRPALRHARRCYGHLAGELGVALWNHCVDHGLLREVGAGGWQLTPPGAAA
                     L"
     gene            complement(503..835)
                     /locus_tag="VFV25_12260"
     CDS             complement(503..835)
                     /locus_tag="VFV25_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HEY1394141.1"
                     /translation="MTIVCFIRYQIDPFQREAFKTYAENWGRIIPRCGGRLLGYFLPH
                     EGSNDIAWGLIAFDSLAAYESYRARLKADPEGRENFAMAQSKRLILREERSFVEAVDG
                     THAIPLLV"
     gene            complement(852..1424)
                     /locus_tag="VFV25_12265"
     CDS             complement(852..1424)
                     /locus_tag="VFV25_12265"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEY1394142.1"
                     /translation="MWAVIFEVQPYAAHEAAYLDTAASLLPRLQQMPGFVSVERFRSE
                     TRPGRLLSLSKWQDEAALRGWREMPEHRAAQRKGRERLFEDYRLRVGELRSLEAGEAQ
                     GWCAVLEWAGDGLPWPDALAGLERYRSLKQEHGRAAIGSVQGTQALAACRLLLGDERA
                     IACGLKLSLCTVNRDYGLNDRAQAPHENRT"
     gene            1536..>2302
                     /gene="gluQRS"
                     /locus_tag="VFV25_12270"
     CDS             1536..>2302
                     /gene="gluQRS"
                     /locus_tag="VFV25_12270"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEY1394143.1"
                     /translation="MVSSTAPTPPGYIGRFAPSPTGPLHAGSLVAALASWLDARAHGG
                     RWLVRIEDVDIPRCVPGADQEILRQLNALGLRPDEPPLWQSQRSALYQAALNQLVASG
                     WAYACGCTRKDIELALAAKGGGRHRHGELVYPGTCRPSEGGLKGKAPRSMRLAAANRP
                     ADRMGAAPLVVDWTDRRLGPQRQDVTHEAGDFVLHRADGLWAYQLAVVVDDAEQGITD
                     VVRGEDLADNTPRQIHLQRCLGLPTPRYMHTPLVLGA"
BASE COUNT          342 a          837 c          783 g          340 t
ORIGIN      
        1 cagcgctgcg gcacccggag gcgtgagctg ccatcccccc gcgcccacct cacgcagtag
       61 gccgtggtcc acacagtgat tccacagcgc gacccccagc tcgccggcca gatggccgta
      121 gcaacgccgc gcatggcgca gggccggtcg cgggagcctg cgcggcacac cggcctgggg
      181 caggcttgca tcgggctgct gcaggttgat ggccccgagt gcctcgacgg cgcgggccac
      241 gtcctcgcgc tgcagcgcga agtagcgatg ccggccctgc acgcgcaacg cgaccacgcc
      301 ggcatccacc agccgcgcca ggtgattgct cgccgccgac ggcgccagct cggctacttg
      361 cgccagctcg gtggccgtgt aggcacgacc gtcgcccaag gcccacagca tgcgcaggcg
      421 cttgggctcg gcgagcaggc gggccacgtc gtcgagctgg ggcgggcgat catcaaggtt
      481 cattcaggca tctccggacg cactacacaa gcaaaggaat cgcatgcgtg ccgtccaccg
      541 cttcgacaaa gcttctttcc tcgcgcagga tcaggcgctt gctctgtgcc atcgcgaaat
      601 tctcgcgccc ttccgggtcg gccttcaggc gggcgcgata gctttcgtag gccgccaggc
      661 tgtcgaaggc gatcaggccc caggcgatgt cgttgctgcc ttcgtgcggc aggaaatagc
      721 cgagcagccg cccgccgcag cgcgggatga tgcggcccca gttctcggca taggtcttga
      781 aggcctcgcg ctggaagggg tcgatctggt agcggatgaa gcagacgatg gtcatgggtt
      841 ctcctgttcg ttcaggttcg gttttcatgg ggtgcctggg cgcggtcgtt caggccgtag
      901 tcgcggttga cggtgcacag gctcagcttc aggccgcagg cgatggcacg ctcgtcgccg
      961 agcagtagcc tgcaggcggc cagcgcctgg gtcccctgca cgctgccgat ggctgcacgc
     1021 ccgtgctctt gcttgaggct gcgatagcgc tccagtcccg cgagagcgtc gggccagggc
     1081 aggccgtcgc ccgcccattc caggacagcg caccagcctt gcgcctcgcc cgcctccagg
     1141 ctgcgcaact cgccgacccg cagccggtag tcctcgaaca ggcgctcccg ccccttgcgc
     1201 tgggcggcgc ggtgctccgg catctcgcgc cagccgcgca gcgcggcctc gtcctgccat
     1261 ttcgacagcg acagcaggcg gccgggccgc gtctcgctgc gaaagcgctc caccgacacg
     1321 aagccgggca tctgctgcag gcgcggcagc aatgaggccg cggtgtcgag gtaggccgcc
     1381 tcgtgagcgg cgtagggctg cacttcaaag atcacggccc acatggggtc tccttagcaa
     1441 gaatcacagg ccgccatcct gcgccgccgg gctggcctga tgattcattc ccgattgaag
     1501 cgtcgcgcga cccgccacaa tgcgctcctg ccttcatggt ttcctcgacc gccccgacac
     1561 ctcccggcta catcggccgc ttcgcgccct cgcccaccgg accgctgcac gccggctcgc
     1621 tggtcgcggc gctggcgagc tggctcgacg cgcgagccca tggcgggcgc tggctggtgc
     1681 gcatcgagga cgtggacata ccgcgctgcg tgcccggcgc cgaccaggaa atcctgcgcc
     1741 agctgaacgc cctgggctta cggccggacg agccgccgct gtggcaaagc cagcgcagcg
     1801 cgctctatca ggctgcgctg aaccagctcg tcgccagcgg ctgggcctat gcctgcggct
     1861 gcacgcgcaa agacatcgag ctggcgctgg ccgccaaagg cgggggacgc caccgccacg
     1921 gcgaactggt ctaccccggc acctgccggc ccagtgaggg cgggctcaag ggcaaagcgc
     1981 ctcgctccat gcggctcgcg gcggcgaacc ggccagcgga ccgcatgggc gcggccccgc
     2041 tcgtcgtcga ctggaccgac cgccggcttg ggccccagcg gcaggacgtg acgcacgagg
     2101 cgggcgactt cgtgctgcat cgcgccgacg ggctctgggc ctatcagctc gcggtcgtgg
     2161 tcgacgacgc cgagcaaggc atcaccgacg tggtgcgcgg cgaagacctg gccgacaaca
     2221 cgccgcgcca gatccatctg cagcgctgcc tgggcctgcc cacgccgcgc tacatgcaca
     2281 cgccgctggt gctgggcgcc aa
//
LOCUS       DASUCY010000618         2830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1154828, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000618 DASUCY010000000
VERSION     DASUCY010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154828"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VFV25_12275"
     CDS             complement(<1..384)
                     /locus_tag="VFV25_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013209157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY1394144.1"
                     /translation="MSSASAHTDSFARDRLPPPELMPELIFTLPELQFPERLNCATEL
                     LGRWVAAGEGGRACVIGAGLRWTYAELQAQANRIANVLIHEMGLQPGNRVLLRAPNTP
                     MLAACWFAVVKAGGIAVGTMPLLRSR"
     gene            complement(381..899)
                     /locus_tag="VFV25_12280"
     CDS             complement(381..899)
                     /locus_tag="VFV25_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003274487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEY1394145.1"
                     /translation="MQNELLDAGRIGAEARAAQDAHLALRLWLRLLASSTQIETEIRR
                     RLRTRFGITLARFDYLAQLHRHPEGLRMNALSRYLMVTGGNVTSLTDELEKEGLVQRE
                     SDPADRRSWRLLLTDAGRSSFERMAGEHEAWVIEIFQGLSPSELDGLYELLGRLRLQQ
                     AHRQTDQDRENA"
     gene            1115..2053
                     /gene="rpoH"
                     /locus_tag="VFV25_12285"
     CDS             1115..2053
                     /gene="rpoH"
                     /locus_tag="VFV25_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEY1394146.1"
                     /translation="MTSSSGASPATALALRDPWALVPSLGNLDAYISAVNRIPLLTQE
                     EEVSLAKRLHEHNDLEAAGRLVLSHLRLVVSVSRQYLGYGLPHGDLIQEGNVGLMKAV
                     KRFDPAQGVRLVSYALHWIKAEIHEYVLKNWRMVKVATTKAQRKLFFNLRSMKQGFKG
                     EAADSDLHRSTLTDSEIDIVARQLNVKREEVIEMETRLSGGDVALEPVGSDDGEESFA
                     PIAYLADESREPTRVIEAQRRDALTADGINQALEVLDPRSRRIVEERWLKVNDDGSGG
                     MTLHELAAEYGVSAERIRQIEVGAMKKMRKALTAYA"
     gene            complement(2117..2716)
                     /locus_tag="VFV25_12290"
     CDS             complement(2117..2716)
                     /locus_tag="VFV25_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEY1394147.1"
                     /translation="MKSIRRHLLLAAVVALGLAACKPAPQVAFKGVDITGADYARELS
                     LPDVDGKPRSLADFKGKAVLVFFGYTQCPDVCPTTMAELAEVRRQLGPDGERVQGVFV
                     TLDPERDTAALLKNYVAGFDPSFVALRGTPEQTQAAAKAFKVFYEKVPSKDGSGNYTL
                     DHTAAAYLFDPQGRVRVFERYGTGPQDLAADFKLLLAGS"
     gene            complement(2713..>2830)
                     /locus_tag="VFV25_12295"
     CDS             complement(2713..>2830)
                     /locus_tag="VFV25_12295"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830749.1"
                     /note="converts protoheme IX and farnesyl diphosphate to
                     heme O; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HEY1394148.1"
                     /translation="SDALARKTFRFSILHLSLLFAALLLDHYLDPILKGWFA"
BASE COUNT          512 a          903 c          939 g          476 t
ORIGIN      
        1 gcgcgagcgc agcagcggca tcgtgcccac cgcgatgccg ccggccttga ccaccgcgaa
       61 ccagcaggcc gccagcatcg gcgtgttggg cgcgcgcagc agcacgcggt tgcccggctg
      121 caagcccatt tcgtggatca agacgttggc gatgcggttg gcctgggcct gcagctccgc
      181 ataggtccag cgcaggccgg cgccgatcac gcaggcccgc ccgccttcgc cggcggcaac
      241 ccagcggccc agcagctccg tcgcgcaatt caggcgctcg gggaactgca gctcgggcag
      301 cgtgaagatc agctcgggca tcagctcagg cggtggtaga cggtcgcgcg cgaagctgtc
      361 ggtatgtgcg gaagcggaac tcatgcgttc tcccggtctt gatcggtttg gcgatgggcc
      421 tgttgcaggc gcaggcgccc cagcaattcg tacaagccgt cgagctcgga cgggctcagg
      481 ccctggaaga tctcgatcac ccaggcctcg tgttcgccgg ccatgcgctc gaagctggat
      541 cggcccgcat cggtgagcaa gagccgccag gagcgccggt cggcgggatc gctttcgcgc
      601 tgcaccaagc cttccttctc cagctcgtcg gtgaggctag tgacgttgcc gccggtcacc
      661 atgaggtagc gcgacaaggc gttcatgcgc aggccttcgg gatggcggtg caactgcgcc
      721 aggtagtcga aacgagccag cgtgatgccg aagcgtgtgc gcagccggcg gcggatctcg
      781 gtctcgatct gggtgctgct ggccagcagc ctcagccaca ggcgcaaggc caggtgggcg
      841 tcttgcgcgg cgcgcgcctc ggcaccgatg cggccggcat ccagcagctc gttttgcaag
      901 ggcaatggag agtgctcgct cacatcgatc ctggatacgg agattcagtt cacttaacga
      961 taatttataa atgaactatt tatctggtat tcgcgttttc ccggatcact aatgaacttt
     1021 cctgatcaca gcgttcaaat gcttgcctta gcactcgctc ataccgagtg ctaatatttg
     1081 cccaaacgga ggttcatgca tgtccctgac tgcgatgacg agttcttctg gcgcttcccc
     1141 ggcgacggct ctggccctgc gcgacccctg ggcgctggtg ccctcgctgg gcaacctgga
     1201 cgcctatatc tcggcggtga accgcatccc cctgctgacg caggaggaag aagtctcgct
     1261 ggccaagcgc ctgcacgagc acaacgatct ggaagccgcg gggcggctgg tgctgtcgca
     1321 cctgcgcctg gtggtctcgg tgtcgcgcca atacctgggc tatggcctgc cgcatggcga
     1381 cctgatccag gaaggcaacg tcggcctgat gaaggccgtc aagcgcttcg acccggccca
     1441 gggcgtgcgc ctggtcagct atgcgctgca ctggatcaag gccgagatcc acgaatacgt
     1501 gctgaagaac tggcgcatgg tcaaggtcgc gacgaccaag gcccagcgca aactgttctt
     1561 caatctgcgc tcgatgaagc agggcttcaa gggcgaggcg gccgatagcg atctgcaccg
     1621 cagcacgctg accgactccg agatcgacat cgtcgcgcgc cagctcaatg tcaagcgcga
     1681 ggaagtgatc gagatggaga cgcgcctgtc gggcggcgac gtggcgctcg agccggtcgg
     1741 cagcgacgac ggcgaggaaa gcttcgcgcc gatcgcctac ctggccgacg agagccgcga
     1801 gccgacgcgg gtcatcgaag cgcagcgccg cgacgcgctg accgccgacg gcatcaatca
     1861 ggctctggag gtgctggacc cgcgcagccg ccgcatcgtc gaggagcgct ggctcaaggt
     1921 caacgacgac ggctccggcg gcatgacgct gcacgagctg gcggccgaat acggcgtcag
     1981 cgccgagcgc atccgccaga tcgaagtggg cgcgatgaag aagatgcgca aggcgctcac
     2041 tgcctacgcg tgaacagcgc tacaaaatca aaaatgcctt tcggcaaaat cacccgggct
     2101 tccgggtgat tttttatcag cttcccgcca gcagcagctt gaagtcggcc gccaggtcct
     2161 gcggtcccgt gccatagcgc tcgaacaccc gaacccgccc ctgcgggtcg aagaggtagg
     2221 ccgcggccgt gtggtccagc gtgtagttgc cgctgccgtc cttgctcggc accttttcgt
     2281 agaagacctt gaaggccttg gccgccgctt gcgtctgctc cggcgtgccg cgcagtgcga
     2341 cgaagctcgg gtcgaagccg gccacatagt tcttgagcag tgccgccgtg tcgcgctcgg
     2401 gatcgagcgt cacgaaaacg ccctgcacgc gctcgccgtc ggggcccagc tggcgcctga
     2461 cctcggccag ttcggccatc gtggtcgggc agacatcggg acattgcgtg tagccgaaga
     2521 agaccagcac ggccttgcct ttgaagtcgg ccaggctgcg cggcttgccg tccacgtcgg
     2581 gcaggctgag ctcgcgggcg tagtcggcgc cggtgatgtc cacgcccttg aaagccacct
     2641 gcggcgcagg cttgcaggcc gcaagcccca gtgcaacgac ggcagccagc agaaggtggc
     2701 ggcgaatgga cttcatgcga accagccctt cagaatggga tcgagatagt ggtccagcag
     2761 cagcgcggcg aagagcagcg acagatgcag gatggaaaat cggaaagtct tgcgtgccag
     2821 cgcatcgctg
//
LOCUS       DASUCY010000619         5550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1026176, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000619 DASUCY010000000
VERSION     DASUCY010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 5550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5550
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026176"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="VFV25_12300"
     CDS             complement(<1..808)
                     /locus_tag="VFV25_12300"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY1394149.1"
                     /translation="MFIVGNDGVIACDAPPTLGLSYPAAIAEVTPLPVTHLIYSHEHV
                     DHIGASTVFPAGIEIIASQATARLLRERNDPLRRLPTRTFQDRLDVTISGQRLELAHR
                     GANHSVDNSFIYAPRQKVLMFVDVIYPGWVPYKNLGVAVDIPGFVAAHRQALAYPFKT
                     MVAGHVSRPGTRADVLIQMELLRDLADAARRAYESLSFADFLAANPPAAGQTAWDRHN
                     DYEQLLVDKMANALLSAWSTRLQGAATYLRDNCWAMLESFVVQGRPNPQAL"
     gene            complement(1012..1587)
                     /locus_tag="VFV25_12305"
     CDS             complement(1012..1587)
                     /locus_tag="VFV25_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1394150.1"
                     /translation="MSHHLIAVAALLSMADFAFGAPAREDGLEQWRRIESVLTHARCL
                     NCHTSTDYPRQGDDRHRHAFEVVRGADGHGAAAAQCAMCHHDVNADAAGVPGAKGWRL
                     APPAMSWERAPGIPMTSHELCRTLTDKRRNGGRSAAQLIEHHADEPLVRWAWSPGHHP
                     DGTPRATPPLSQVDFVTATRIWAAAGAPCPQ"
     gene            complement(1584..2045)
                     /locus_tag="VFV25_12310"
     CDS             complement(1584..2045)
                     /locus_tag="VFV25_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEY1394151.1"
                     /translation="MKFEVNGRGVEVDVPGEEPLLWVLREELGLTGTKFGCGIAACGA
                     CTVHVDGQAVRSCSFPAQLAEGRRITTIEGLAKDRSHPVQVAWIEHQVPQCGYCQSGM
                     IMAAAALLEVIPKPTDQDIDRSITNICRCGTYSRVRAAIHSAALRAAGGKA"
     gene            complement(2066..4258)
                     /locus_tag="VFV25_12315"
     CDS             complement(2066..4258)
                     /locus_tag="VFV25_12315"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEY1394152.1"
                     /translation="MSLSVVCEVSGWSRRRFLKSSALAAGGFMLSATWRVDAAQLVGA
                     AAASPREGEIGLNAWVRIQSDNTVTVVVSQAEIGQGISTTLPAVLVDELGADWSTVRL
                     ETAPYDPAYANPRYKWMFTGNSESIQAFYDLMRSMGASARAMLLAAASERFAVPVSEL
                     KAERSFVEHAASGRRASFGELATAAARQLPPDKPQLKSDSSLTLIGKPLAKVDVPSKV
                     DGSAIFGIDFRVPGMLHAAVRTAPAFGARLELRNRDALLKEKGVRAVLPITGGFAVVA
                     DSYWHARSAMLKAEIDTVGAPQPLADSAALREAYRERLEHGPFATPVHEGEAARLLAD
                     GSQVIEHDYENPFLAHATMEPMNCVAQVSAERCIVWGPLQGQNLAWYALQGALGLKGE
                     QVEVHRTPYAGGGFGRRLLPDFVVQAALLSKAVQAPVKLIWDREEDLRRDFYRPASMQ
                     RLKAALGLDGLPQALAARVVSPTIITPVAPFLAETIQKSGVDPSAMEGMTEWPYAIEH
                     RQVEFHLMQVPMPTSVLRTTGYGPNTFALESFVDELAHAAGQDPIAYRRRLLQHQPRS
                     LRVLDRAAQLAGWGGRLRAGEGRGVAFTIAFGTVLAQVVEVHVSGAEVKVLRVVSVAD
                     PGRVLDPRISAAGIEGGVVFGLAGCKAEVSFADGAIVQNNFHQLAMPYLAESPQLVTE
                     FMQSDAPLGGIGEVSPVTLPAALANAIFSATGRRLRSMPLPRHGLQLA"
     gene            4343..5278
                     /locus_tag="VFV25_12320"
     CDS             4343..5278
                     /locus_tag="VFV25_12320"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY1394153.1"
                     /translation="MITIDINHLRRVDLNLLPVFVALMHERNLTRAAARLNLGQPAVS
                     AALKRLRDAVGDRLFVRGRDGMAPTDRALALMQSLEPALGVIHSALRNKPVFDARQEE
                     RIFRLGMSDNHEHFLMPALMERLRAEAPHVRMVVRPVGWSQAAAALDSGEVELLCGYL
                     PQLASWHCSEPLFEAGFLCMFDRKRVGFTGAMTLARYVALPHLLVSSRGDLEGAVDDE
                     LARVGKARRVVMVTAHFSVLPAVLKRIPAVATLPEPTARHLAREFKLEVCDPPIELRG
                     YQSSLAWLRSREDDPAHGWLREMIKSTAQSVAASR"
     gene            complement(5321..5434)
                     /locus_tag="VFV25_12325"
                     /pseudo
     CDS             complement(5321..5434)
                     /locus_tag="VFV25_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT          888 a         1842 c         1847 g          973 t
ORIGIN      
        1 cgagcgcctg cggatttggc cgcccctgta ccacgaagct ttccagcatg gcccagcagt
       61 tgtcgcgcaa ataggtcgcc gcgccttgca agcgcgtgct ccacgcggaa agcagcgcgt
      121 tcgccatctt gtcgaccagc agttgctcgt agtcgttgtg gcgatcccat gcggtttgcc
      181 ccgcggcggg tgggttggcc gcgagaaagt ccgcgaagct gaggctctca taggcgcgcc
      241 gcgcggcgtc tgccaaatct ctcagcagct ccatctgaat cagcacatct gcccgcgtgc
      301 cgggccggct cacatgcccc gccaccatgg tcttgaacgg ataggccagc gcttgtctgt
      361 gggcagcgac aaagccgggg atatcgacag ctacgcccag gttcttgtac ggaacccagc
      421 cgggatagat gacgtcgaca aacatcagca ccttctgtct cggcgcatag atgaagctgt
      481 tatcgacgct gtggttggcc cctcgatgcg ccagctccag tcgttgcccg ctgatggtca
      541 cgtcgagccg gtcctggaaa gtccgcgtcg gcagccggcg caggggatca ttgcgctctc
      601 gcagaagacg cgccgttgct tgactggcga tgatctcgat ccccgctggg aagacggtcg
      661 aggcaccaat atggtcgaca tgctcgtggc tgtagatcag gtgcgtcaca ggcagcggcg
      721 tcacttcggc gattgccgcc ggatagctca agcccagcgt gggtggcgcg tcgcaggcga
      781 tcacgccatc gttgccgacg atgaacatcg tgctgtaggc gccgtcgctg agccagtaga
      841 tctgctcggc gatcggcttg acgagatagc gtttgccctt gggtagcgcg aagtcgctgt
      901 ccgagggcgt ttctgcacaa gcgcaccagg gcgcaagcag ggcgctcgtg gcggctaggc
      961 tgatgccacc gccgagcagc cagcgccttg gcttcgaagc cggtgccctg gtcattgtgg
     1021 gcagggcgct ccagcagcgg cccagattcg cgtggcggtg acgaagtcga cctggctcag
     1081 gggcggcgtt gcgcgcggcg tgccatcggg gtgatgcccg ggagaccagg cccagcgcac
     1141 cagcggttcg tccgcgtggt gttcaatgag ctgcgcggcg ctgcgcccgc cgttgcggcg
     1201 cttgtcggtg agcgttcggc acagctcgtg gctcgtcatg ggtattcctg gtgcccgctc
     1261 ccaggacatt gccggcggtg cgaggcgcca gcctttcgcg cccggcacgc cggccgcatc
     1321 ggcgttgacg tcgtggtgac acatcgcgca ttgcgccgcg gccgcaccgt gaccatcggc
     1381 gccccgcaca acttcgaagg catggcgatg gcggtcgtcg ccttggcgcg gatagtcggt
     1441 gctggtgtgg cagttcaggc agcgggcatg ggtcagcacc gattcgatcc ggcgccattg
     1501 ctccaggcca tcttcgcgcg ccggagcgcc gaacgcgaaa tccgccatcg acagcaaggc
     1561 tgccacagca atcaagtggt ggctcatgcc ttgcccccgg cagctcgcag tgccgcgctg
     1621 tggatcgccg cgcggacgcg cgaataggtg ccgcagcggc agatgttggt aatcgagcga
     1681 tcgatgtcct gatcggtggg ctttgggatg acctcgagca gcgcggcggc ggccatgatc
     1741 atccccgact ggcagtagcc gcactgcggc acctgatgct cgatccaggc cacctgcacc
     1801 ggatgcgagc gatccttggc caagccctcg atcgtcgtga ttcgcctgcc ctcagccagc
     1861 tgcgccggga agctgcacga acgcaccgct tgtccatcga catgcacagt gcaggcaccg
     1921 caggccgcga tgccacagcc gaacttggtg ccggtgagcc caagctcctc gcgcagcacc
     1981 cacagcaaag gctcttcccc ggggacgtcg acctcgacgc cgcggccgtt gacttcgaat
     2041 ttcacgatgt gtcctccttc cggactcaag ccaattgcag cccgtggcgc ggcagcggca
     2101 tggaccgcag ccgtcgcccg gtggcgctga agatcgcatt ggccagcgcc gccggcaggg
     2161 tcacggggct gacctccccg atgccaccca gcggcgcgtc gctctgcatg aactcggtga
     2221 ccaactgcgg gctctctgcg aggtagggca tggcgagttg gtgaaagttg ttctgcacga
     2281 tggcgccatc ggcaaagctc acctccgcct tgcagcctgc gaggccgaac accacgccac
     2341 cctcgatgcc ggcggcgctg atgcgcgggt cgagcacgcg gccggggtcg gccactgaga
     2401 ccacgcgcag caccttgact tcggcgccgc tgacgtgcac ctcgaccact tgcgccaaca
     2461 ccgtgccaaa ggcgatcgtg aatgcgacgc cgcgaccttc gccggcccgc agacggccac
     2521 cccagcctgc caactgcgcc gcccggtcga gcacgcgcag cgaccgcggc tggtgctgca
     2581 gcagtcgtcg gcggtaggcg atcgggtctt gcccggccgc atgggcgagc tcatcgacga
     2641 agctttcgag cgcgaatgta ttggggccgt agccggtggt gcgcaacacc gaggtcggca
     2701 tcggcacctg catcagatgg aactcgacct ggcggtgctc gatggcatag ggccactctg
     2761 tcatgccctc catggcgctc gggtcgacgc ccgacttctg gatggtttcg gccaggaacg
     2821 gtgccaccgg cgtgatgatg gtcggcgaca ccacgcgcgc tgccagtgcc tgtggcagcc
     2881 catcgagacc gagcgccgcc ttcaggcgct gcatggaggc cggccgatag aagtcgcgcc
     2941 gcaggtcttc ttcacggtcc cagatgagct tgaccggggc ttgcaccgcc ttcgacagca
     3001 gcgcagcctg aaccacgaag tccggcagca ggcggcggcc gaagccgccg cctgcatagg
     3061 gcgtgcggtg cacctcgacc tgctcgccct tcaggcccag tgcgccctgc agggcgtacc
     3121 acgcgaggtt ctggccttgc agcggccccc agacgatgca gcgctcggcg ctgacctgag
     3181 cgacgcagtt catcggctcc atcgtcgcgt gagccaggaa ggggttctcg tagtcgtgct
     3241 cgatcacttg gctgccgtcg gcgagcaagc gcgcagcctc gccctcgtgc accggtgtgg
     3301 cgaaggggcc gtgctcgagg cgctcgcgat aggcctcgcg cagtgccgcg ctgtctgcca
     3361 agggctgcgg agcgccgacg gtgtcgatct cggccttcag catggcgctg cgcgcgtgcc
     3421 agtagctgtc ggccacgaca gcgaagccac ccgtgatcgg cagcactgcg cgtacgcctt
     3481 tctccttgag cagcgcgtcg cgattgcgca gctcgagccg cgcgccgaat gcaggggccg
     3541 tgcgcacggc ggcgtgcagc atgcccggca ccctgaaatc aatgccgaag atcgccgagc
     3601 cgtcgacctt ggacggcacg tcgaccttgg ccagcggctt gccgatcagc gtcagcgagc
     3661 tgtcgctctt gagctgcggc ttgtccggtg gcagctgacg cgcggccgcc gtggccagct
     3721 caccgaagct cgcgcgccgc cccgacgcgg catgctcgac gaagctgcgt tcggccttca
     3781 gttcgctcac cggcacggcg aatcgctcac tggccgctgc cagcaacatg gcacgcgcac
     3841 tggcgcccat cgagcgcatc aggtcataga aggcttggat gctctcgctg ttgccggtga
     3901 acatccactt gtagcgcgga ttggcgtacg cggggtcgta gggggcagtc tccagtctta
     3961 cggtcgacca atcggcgccg agttcgtcga cgagcaccgc cggcaaggtg gtgctgatac
     4021 cttgcccgat ctcggcctgg ctcacgacga cggtcaccgt gttgtcgctc tggatgcgga
     4081 cccatgcgtt gaggccgatt tctccctcgc gcggacttgc ggcggcagcg ccgaccaact
     4141 gcgcagcatc gacccgccaa gtggcgctga gcatgaatcc gccggcggcc aaggcgctgc
     4201 tcttcagaaa gcggcgccgg ctccagcccg agacctcgca gaccaccgac aagctcatgg
     4261 gtcactccta acacaggaac gcttggacaa gctgtcgccg attatttaag ctgcctcagt
     4321 gcaaatcaaa tcgattgcag cgatgatcac tatcgatatc aatcatcttc ggcgggtgga
     4381 cctaaacctg ctgccggtgt tcgtggcgct gatgcacgag cgcaacctga cgcgcgccgc
     4441 cgcgcgcttg aatctgggcc agcctgccgt gagcgccgcg ctgaagcgcc tgcgcgacgc
     4501 agtgggcgat cgacttttcg tgcggggccg cgacggcatg gcgccgaccg atcgcgccct
     4561 cgccctcatg cagagcctgg agcctgcgct cggagtcatt cattcggcgc tgcgcaacaa
     4621 gcctgttttc gatgcccgac aggaggagcg catttttcgg ctcggcatgt cggacaacca
     4681 cgagcacttt ctcatgcccg cgctgatgga gcgcttgcgc gccgaggcac cgcatgtgcg
     4741 catggtggtg cggccggtcg ggtggagcca ggcggctgct gcgctcgaca gcggcgaggt
     4801 cgagttgctc tgcggctatc taccgcagct cgcatcctgg cattgcagcg agcctttgtt
     4861 cgaggctggc tttctgtgca tgttcgatcg caagcgagtc ggcttcaccg gtgcgatgac
     4921 attggcgcgc tatgttgcgc tgccgcatct gctggtgtcg tcccgcggag atttggaggg
     4981 agcggtggac gatgagttgg ccagggtggg caaggcgcgc cgcgtcgtga tggtgacggc
     5041 gcatttctcg gtgctgccgg ccgtgctcaa gcgcatcccg gcagtggcca ccctgccgga
     5101 gcccactgcg cgccatctgg cgcgcgagtt caagctcgag gtctgcgacc cgccgattga
     5161 attgcgcggc taccaaagct ctctggcctg gctgcgctcc cgcgaagatg atccggcaca
     5221 cggctggctg cgcgagatga tcaaaagcac cgcgcaaagc gtcgcggctt cgcgctaggc
     5281 ttgccctgtg aagcccgcct aagccgccag ccccggaaca aaaaacaagt gggcgttgag
     5341 catgtagcgt cttgtgctgc attaccaccg ctcgagaggc cgcctggtcg ccgtggcaga
     5401 tatcccggac tttgaggccg accgggtcac agccgacgag ggccagatcg tgggcttcta
     5461 cgtggtcttt atcggtcggg gttccatcgc cgtccatggg ttcgcacgca gcgccgacgg
     5521 gagtttcaac acgatcgact tccccggcgc
//
LOCUS       DASUCY010000620         2312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_158788, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000620 DASUCY010000000
VERSION     DASUCY010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158788"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            259..1014
                     /locus_tag="VFV25_12330"
                     /pseudo
     CDS             259..1014
                     /locus_tag="VFV25_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003265184.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
     gene            1027..1482
                     /gene="aroQ"
                     /locus_tag="VFV25_12335"
     CDS             1027..1482
                     /gene="aroQ"
                     /locus_tag="VFV25_12335"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma subclass chorismate mutase AroQ"
                     /protein_id="HEY1394154.1"
                     /translation="MRQLIDQRLLLAQDVARAKWNTQGAIEDLPRETQIIRGLGQQAK
                     ALGLPAPWVESFFRAQIEASKTVQRELFARWERERQGRFDDAADLATVIRPKLDVLTA
                     KLMRALADDQAVLKDPARRADVAHALRPLQAAALSGAAADQALAPLVSD"
     gene            complement(1500..>2312)
                     /locus_tag="VFV25_12340"
     CDS             complement(1500..>2312)
                     /locus_tag="VFV25_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HEY1394155.1"
                     /translation="EAHDVSVRGFDGAQPQVRWKKDGREEVLDCDFIAGCDGFHGVCR
                     ASVPAQAIRTFERVYPFGWLGLLSETPPVSHELIYSNHPRGFSLCSMRSTHRSRYYLQ
                     CPLDDKPENWSDEAFWEELRRRLDPQAREHLVTGPALEKSIAPLRSFVAEPMRFGRLF
                     LAGDAAHIVPPTGAKGLNLAASDVGYLSRALAEYFKEKSSAGIERYSEHCLRRVWRAE
                     RFSWWMTSLLHRFPEAGEFGHKIQQAELDYLVGSRAASTVLAENYVGLPLEA"
BASE COUNT          369 a          793 c          802 g          348 t
ORIGIN      
        1 ccagtcctgt cagcccgcta gtgccagcca atgcggcggc ggcggccagc ggggccacgt
       61 cgcgctattc cttcatggtc ggcctgcgcc atcggttctg agccaagccg atacgagcca
      121 aggggcacct cagggtgccc ctttttcatc gcccctgcgg ccggcaccgc acaatggcac
      181 gatcccccgc cgaaggagac gcttgtgaac ctctgctcgt cacgcccgcg cagcttgctg
      241 gccggcctgg ccgcttcgct gagcctgctg gtggccgctc cgctcgtctt cgccgccgac
      301 gccgccagcc gcctcgacga gatcgtcgcg cgcggcagcc tgcgcgtggg cacgacgggc
      361 gactacaagc cgttcagctt ccgaccagcg ccgaatgccg acttcgtcgg cctcgacatc
      421 gaactggccg gcgacctggc ccgttcgctg ggcgtcaagc tggagatcgt gccgaccagc
      481 tggcccgcgc tgatgaagga cttcggcgag gaccgcttcg acctcgcgat cggcggcgtc
      541 tcggtcacgc tggagcggca gaagaagggc ctgttctcgc tgccctatat gcgcgacggc
      601 aagacgccga tcacgcgctg cgagaaccag gccaagtacc agaccctggc gcagatcgac
      661 caggcgaacg tgcggctgat cgtcaacccg ggcggcacca acgagcgctt cgcgcgcgcc
      721 tatgtgcagc atgcccagct ggcggtctat ccggacaacg tgagcatctt cgagcagatc
      781 gtcgacggca aggccgacct gatgatcacc gatgcgatcg aggcgcggct gcagtcgcgc
      841 atccatccgc agctgtgcgc cgtgcatccc gatgcgccgt tcgacttctc ggagaaggcc
      901 atcctgctgc cgcgcgacgg ggtgttcaag gcctatgtcg accaatggct gcaccaggac
      961 agcgccagcg gcgcgctgca gaagcgcatc gatcgctggc tggcctatcc ctgggcgctg
     1021 gacggcctgc gccagctgat cgaccagcgg ctgctgctgg cccaggacgt ggcgcgtgcc
     1081 aaatggaaca cccagggcgc gatcgaggac ctgccgcgcg agacgcagat catccgcggc
     1141 ctggggcagc aggctaaggc gctaggcctg cccgctccct gggtcgagag cttcttccgc
     1201 gcgcagatcg aggcctcgaa gacggtgcag cgcgagctct tcgcgcgctg ggagcgcgag
     1261 cggcaaggca ggttcgacga cgcggcggac ttggccacgg tgatccgccc caagctggat
     1321 gtgctgactg ccaagctaat gcgcgccctg gccgatgacc aggccgtgct gaaggatccg
     1381 gcgcgccgcg ccgacgtggc gcatgccttg cggccgctgc aggccgcagc gctcagtggc
     1441 gcggccgcgg atcaggcgct ggcgcccttg gtcagcgact gatatgagag cgcagcggct
     1501 caggcttcga gcggcaatcc cacgtagttc tcggccagca cggtcgaggc cgcgcgggag
     1561 ccgaccagat agtccagctc ggcctgctgg atcttgtgtc cgaactcgcc ggcttcggga
     1621 aagcggtgca gcagcgaggt catccaccac gagaagcgct cggcgcgcca gacgcggcgc
     1681 aggcagtgtt cggagtaacg ctcgatgccg gcgctgcttt tctccttgaa gtattcggcc
     1741 agcgcgcgcg aaagatagcc cacgtccgag gccgcgaggt tgaggccctt ggcgccggtg
     1801 ggcggcacga tgtgggcggc gtcgccggcg aggaagaggc ggccgaagcg catcggctcg
     1861 gcgacgaaac tgcgcagcgg cgcgatgctc ttctccagtg cagggccggt cacgagatgc
     1921 tcgcgggcct gcgggtccaa gcggcgccgc aactcttccc agaaggcctc gtccgaccag
     1981 ttctcgggct tgtcatccag agggcactgc aggtagtagc ggctgcggtg cgtggagcgc
     2041 atgctgcaca gcgagaagcc gcgcggatgg ttggagtaga tgagctcgtg cgacaccggc
     2101 ggcgtctcgg acagcagccc gagccagccg aacgggtaca cgcgctcgaa ggtgcggatg
     2161 gcctgcgccg gcacgctcgc gcggcacacg ccgtggaagc cgtcgcagcc ggcgatgaag
     2221 tcgcagtcga ggacctcctc gcggccgtcc ttcttccagc gcacctgcgg ctgcgcgccg
     2281 tcgaagccgc gcacgctcac gtcgtgcgcc tc
//
LOCUS       DASUCY010000621         2020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_110865, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000621 DASUCY010000000
VERSION     DASUCY010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110865"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..427
                     /gene="tolR"
                     /locus_tag="VFV25_12345"
     CDS             38..427
                     /gene="tolR"
                     /locus_tag="VFV25_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HEY1394156.1"
                     /translation="MSEINMVPFIDVMLVLLIIFMVSAPLITTGLVDLPSVGQAKRQP
                     EHVIEVVLGSDESLKLRVDGKDAGERATLKTLAARVNDVQGGSRNTPVIISADRSVKY
                     EAVVKVMDTLNRAGVQRVGLAVKTTGA"
     gene            458..1255
                     /gene="tolA"
                     /locus_tag="VFV25_12350"
     CDS             458..1255
                     /gene="tolA"
                     /locus_tag="VFV25_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HEY1394157.1"
                     /translation="MNAVTLTHDALRPQPPDRMGSGLLLAFAVHALLLLALALGVHWH
                     NAEPAGVEAELWAAVPQVAAPRAVAPEPAPTPEPPKPAVQTPPPPPVQREADIAIEKA
                     KKLQEQQKAREEEEKKIQQEKEKKAEAARKEADQKRQDKLMADLRAQEMARINNALQG
                     TGGPTSTGSAARSAGPSASYAGRIKARIKPNIVYTDAIVGDPAADVEVKVAPDGTIIG
                     RRLIKSSGVKEWDEAVLRAIDRTEVLPRDVDGRVPPTIEILFRARDL"
     gene            complement(1283..>2020)
                     /locus_tag="VFV25_12355"
     CDS             complement(1283..>2020)
                     /locus_tag="VFV25_12355"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY1394158.1"
                     /translation="QHFSQLWPRLRALAEARPLCIVVIGGDVLAVELAFALERALGRD
                     SRISLVSGSGGLLPGFADSAQRRVRDKLRQRRITLFDEDCVRIEAQQILLASGLKLAC
                     DAPVIAAGAGAPAWLRDSGLALAADGQVDVNELLQSRSHPEVFAALGGLAGREAAGAG
                     EGLAINLRRHIAGGVLQPQRTGASTVRWLSFSDGKALALRGRHSATARWASWWKDYAD
                     RRYMAAWRVKAAGLPKKLPAVDKSEER"
BASE COUNT          359 a          733 c          677 g          251 t
ORIGIN      
        1 cgcgctcgta caacgccacg gctcgcgccg ccgcaccatg agcgagatca acatggtgcc
       61 cttcatcgac gtgatgctgg tgctgctcat catcttcatg gtcagcgccc cgctcatcac
      121 caccggcctc gtggacctgc cctcggtggg ccaggccaag cgccagcccg agcatgtgat
      181 cgaggtggtg ctgggcagcg acgagagcct caagctgcgc gtggacggca aggacgccgg
      241 cgagcgcgcc acgctcaaga ccctggccgc gcgcgtcaac gacgtccagg gcggcagccg
      301 caacacgccg gtgatcatct cggccgaccg cagcgtcaag tacgaggcgg tggtcaaggt
      361 gatggacacg ctcaaccgtg ccggcgtgca gcgcgtcggc ctggcggtca aaaccacggg
      421 cgcctgagcc catagcgcgc cgccctctga tcgagccgtg aacgccgtca ccctgaccca
      481 cgacgcattg cgcccccagc cgccggaccg catgggcagt ggcctgctgc tggcctttgc
      541 ggtgcacgcc ctgctgctgc tcgcgcttgc cttgggcgtg cattggcaca acgccgagcc
      601 ggccggcgtg gaggccgagc tctgggccgc cgtgccgcaa gtcgctgcgc ctcgcgccgt
      661 agcgcccgaa cctgcgccga cgcccgagcc gcccaagccc gccgtgcaga ccccgccccc
      721 gccgcccgtg cagcgcgaag ccgacatcgc catcgagaag gcgaagaagc tgcaggagca
      781 acagaaggcg cgcgaggaag aagagaagaa gatccagcag gaaaaggaga agaaggccga
      841 ggcagcgcgc aaggaggccg atcagaaacg gcaggacaag ctgatggcgg acctgcgggc
      901 ccaggaaatg gcgcgcatca acaacgcgct gcagggcacg ggcggcccga ccagcacagg
      961 ctccgcggca cgcagcgccg ggccgtcggc cagctatgcg gggcgcatca aggcgcgcat
     1021 caagcccaat atcgtttaca ccgacgcaat cgtcggcgac ccggccgccg acgtggaagt
     1081 gaaggtcgcg ccggacggca ccatcatcgg ccgccgcctg atcaagtcca gcggcgtcaa
     1141 ggaatgggac gaggccgtgt tgcgtgcgat cgaccgcacc gaggtgctgc cgcgcgatgt
     1201 ggacggccgc gtgccaccga cgatcgagat cctctttcgc gccagggacc tctgagcctg
     1261 atccccttcc ggggcggcag cctcaacgct cctcggactt gtcgacggca ggcagcttct
     1321 tgggcaggcc cgcggccttg actcgccacg cggccatgta gcggcgatcg gcatagtcct
     1381 tccaccagct ggcccagcgc gccgtagctg aatgccggcc gcgcaacgcc agagctttgc
     1441 catcgctgaa ggacagccag cgcaccgtgc tggcgccggt gcgctgcggc tgcagcacgc
     1501 cgccggcgat atggcgccgc aaattgatcg ccagaccctc gccggccccg gcagcctcgc
     1561 gccccgccaa gccgccaagg gccgcaaaga cctcgggatg cgagcggctc tgcagcagct
     1621 cgttgacgtc cacctgccca tcggccgcca aggccaagcc gctgtcgcgc agccatgccg
     1681 gagccccggc gccagcggcg atcacgggcg cgtcgcaggc cagcttaagg ccgctcgcca
     1741 gcaggatctg ctgggcttcg atccgaacgc agtcctcgtc gaacaacgtg atgcggcgct
     1801 ggcgcagttt gtcccgcacg cgacgctggg cactgtccgc aaagccaggc aggagaccac
     1861 cgctgccgct caccaggctg atgcgactgt cgcggcccag cgcgcgctcc agtgcaaagg
     1921 ccagctcgac ggcgagcacg tcgccgccga tcacgacgat gcagagcggt cgcgcctcgg
     1981 ccagcgcgcg cagccgcggc cacagctgtg agaaatgctg
//
LOCUS       DASUCY010000622         4371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1267186, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000622 DASUCY010000000
VERSION     DASUCY010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1267186"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..3403)
                     /locus_tag="VFV25_12360"
     CDS             complement(173..3403)
                     /locus_tag="VFV25_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HEY1394159.1"
                     /translation="MPEQRFPPKLRPERPPGTLLPLAPGLPGYAELHCRSNFSFLTGA
                     SHPEELVARALELGYAGLGLTDECSVAGVVRAHTAIKGWSDEQKKSFRFIVGSEFDVQ
                     PAGDTPGCRLVLLAQTRQGYGNLSELITLARLRAGKGEYRLDVRDLDSITPGSEELCG
                     MPECWALLIPRREDPPEVLQAQAHWLKATFGERARIAIELLMWADDASLAERLKALSA
                     TTGIPLAAAGDVLMHVRSRKPLQDVLTATRLKQPVAECGFALARNAEQHLRSRLKLAQ
                     LYRQEWLEETVRMAEACRFSLAELGYEYPDEIVPEGATAIGHLRKLTEQGMHERYPDG
                     VPDQVEAQIEKELELIAYKRYEAFFLTVHDIVRFARSKEILCQGRGSAANSAVCYCLG
                     ITAVNPMDTRLLFERFISRERDEAPDIDVDFEHERREEVIQYVYGKYGQHRTALAAAL
                     ATYRTRGAVRDVGKALGLDPQLIDAMAKSFQWFDDKDEMLRRFVELGIDPAASVTRHW
                     VELIGTLQGFPRHLSQHTGGFVIARDKLSRMVPIETAAMENRRVIQWDKDDLESLGLL
                     KVDVLALGMLTVLRRSLDFYRQWRGREMQLYGVPQDDEPTYDMICKADTVGVFQIESR
                     AQQSMLPRLRPRKLYDLVIEVAIVRPGPIQGGMVHPYLKRRDAQRAGKLEPSPYPQLD
                     VALERTLGIPIFQEQVMQICVIAAGFTPGEADELRRAMAAWKRKGGVHKYRDRILEGM
                     KAKGYDEAFAEAIFKQIMGFGDYGFPESHAYSFALLAYVSSWLKCHEPAIFLAAMLNS
                     QPMGFYGPSQLVQDAKRHGVEVRPVDVVHSDHDCTLEPRADSEHPAVRLGLCMISAVS
                     KESTERLMAARREHPFVSIEDLALRARIDTRELQALARADALASLAGHRREQTWIASG
                     QELPPALLAEAPTGEAALALPQAPEGEEILLDYAATGLSLRRHPLALLRPRLARRGWR
                     SADELKQARDGQSIWACGIVTMRQQPQTAKGTIFVTLEDETGSVNVIVWERVRKQHRH
                     ALLRSRLLAVAGEWQNRENVPHLIARRLVDVTPWLGRLATTSRDFH"
     gene            complement(3424..>4371)
                     /locus_tag="VFV25_12365"
     CDS             complement(3424..>4371)
                     /locus_tag="VFV25_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HEY1394160.1"
                     /translation="QPPRLAELLQAIGCRRLGDVRALPRAGLQRRGGMALLQALDRAY
                     GDAPDPRDWFAAPPEFHAELELMHRVDQAEALVFAAQRLLQPLCGWLSRQWLAATRVR
                     LVLRHERGRHAAPDTLLRIELGQATRDPQQLQTLLRERLHRLTLQAPVYWLRLVLEEA
                     APCPGAEGQLLPDPGREAQDFLALLDRLRARLGAEQVQHPALRADHRPELAHVLLSRQ
                     PDRLAQQCADVPQLPRPAWLLPVPLRLREQGERPVHGSTVLNLLTRAERIEAGWFDGK
                     LVCRDYHVAEGSDHRLRWVYCERRGHGGERAWFLHGLFA"
BASE COUNT          675 a         1467 c         1523 g          706 t
ORIGIN      
        1 acatcgatgc ggggcgggtc ttgttgatcc tcgacgtacc cgtgaagcgc gtcgacgaga
       61 tacgcgcctt gctggaggct cggcatcccg aggctcgttg gggcgggctg gagacgagca
      121 tcccagcgtt tccctgagcg caaaacaaag cactcgcgcg gctcgttgct tgtcagtgaa
      181 agtcgcgcga agtcgtcgcc aggcgcccga gccagggcgt cacatcgacc aggcggcggg
      241 cgatcaggtg gggcacgttt tcgcggttct gccattcgcc ggccacggcc agcaggcgtg
      301 agcgcaatag cgcatggcgg tgctgcttgc gtacccgctc ccatacgatc acattgactg
      361 aaccggtttc gtcctccagt gtgacgaaga tcgtgccttt ggcggtctgg ggctgctggc
      421 gcatggtaac gatgccgcag gcccagatgg actggccgtc acgtgcctgc ttcagttcgt
      481 cggcgctacg ccagccgcgg cgcgcgaggc gtgggcgcag cagcgccagc ggatggcggc
      541 gcaggctcaa gcccgtggcg gcgtagtcca gcaggatttc ttcgccttcc ggcgcctggg
      601 gcaaggccag cgcggcctcg ccggtaggcg cctcggcgag caaggccggg ggcagctcct
      661 ggcccgaggc gatccaggtc tgctcgcggc gatggccggc gaggctcgcg agcgcgtcgg
      721 cgcgggccag ggcctgcagc tcgcgcgtgt cgatgcgggc gcgcagcgcg aggtcttcga
      781 tgctgacgaa agggtgctcg cgccgtgccg ccatcaggcg ctccgtgctc tccttggaaa
      841 ccgctgaaat catgcacagg cccaggcgca cggcgggatg ctcgctgtcg gcgcgcggct
      901 ccagcgtgca atcgtggtcg ctgtgcacca cgtcgaccgg gcgcacctcc acgccatggc
      961 gtttagcgtc ctgcaccagc tgcgacggcc catagaagcc catcggctgc gagttcagca
     1021 tcgccgccag gaaaatcgcg ggctcgtggc acttcagcca gctgctcaca taggcgagca
     1081 gcgcgaagct gtaggcgtgg ctttcgggga agccgtagtc gccgaagccc atgatctgct
     1141 tgaagatggc ttcggcgaag gcttcgtcat agcccttggc cttcatgccc tcgaggatgc
     1201 gatcgcggta tttgtgcacg ccgcccttgc gcttccaggc agccatggcc cggcgcagct
     1261 cgtcggcttc tcctggcgtg aagccggcgg cgatcacgca gatctgcatc acctgttcct
     1321 ggaagatggg gatgcccagc gtgcgctcga gggcgacgtc gagctgcggg tagggcgagg
     1381 gttcgagctt gcccgcgcgc tgtgcgtcgc ggcgcttcag ataaggatgg accatgccgc
     1441 cctggatcgg ccccggccgc acgatggcga cctcgatcac caggtcgtag agctttctgg
     1501 ggcgcagccg cggcagcatg ctttgctggg cccggctttc gatctggaac acgccgacgg
     1561 tgtcggcctt gcagatcatg tcgtaggtcg gctcgtcgtc ctgcggcacc ccgtagagct
     1621 gcatctcgcg gccgcgccac tgccggtaga aatcgagcga acggcgcagc acggtcagca
     1681 tgcccagtgc cagcacgtcc accttgagca ggcccaacga ctcgagatca tccttgtccc
     1741 attgaatgac gcggcggttt tccatcgccg cggtctcgat gggcaccatg cgcgagagct
     1801 tgtcgcgcgc gatcacgaag ccgccggtgt gctgcgacag atggcgcgga aagccctgca
     1861 gcgtgccgat cagctccacc caatgccggg tgacgctggc cgccggatcg atgccgagct
     1921 cgacgaaacg cctgagcatt tcgtccttgt cgtcgaacca ttggaaggac ttggccatcg
     1981 catcgatgag ctgcgggtcc agtcccaggg ccttgcccac gtcgcgcacc gcgccgcgcg
     2041 tgcggtaggt ggccagcgcc gccgccaggg ccgtgcggtg ctggccgtac ttgccgtaga
     2101 cgtattggat gacctcttcg cggcgctcat gctcgaaatc cacgtcgatg tcgggcgcct
     2161 cgtcccgctc gcgggagatg aagcgctcga acagcaggcg cgtgtccatc gggttcactg
     2221 ccgtgatgcc caggcagtag cacacggcgg aattggccgc ggagccgcgc ccctggcaaa
     2281 ggatctcttt ggagcgggcg aaacggacga tgtcgtgcac cgtcaggaag aaggcctcgt
     2341 agcgcttgta ggcgatgagc tcgagttcct tctcgatctg tgcctcgacc tggtcgggca
     2401 ctccgtcggg gtagcgctca tgcatgccct gctcggtgag cttgcgcaga tggccgatcg
     2461 ccgttgcgcc ctccggcacg atctcgtcgg ggtattcgta gcccagctcg gccagcgaaa
     2521 acctgcaggc ctcggccatg cgcacggtct cttccagcca ctcctgccga tacagctgcg
     2581 ccagcttcag ccgcgagcgc aaatgctgct cggcattgcg cgccagcgcg aagccgcatt
     2641 cggccaccgg ctgcttgagg cgggtagcgg tcagcacgtc ctgcagcggc ttgcgcgagc
     2701 gcacgtgcat cagcacgtct cccgcggcgg ccagcggaat gccggtggtt gcggacaagg
     2761 ccttcagccg ctcggcgagc gaggcatcgt cggcccacat cagcagctcg atcgcgatgc
     2821 gggcacgctc gccgaaggtg gccttcagcc aatgggcctg ggcctgcagc acctcgggcg
     2881 ggtcttcgcg gcgcgggatc agcagcgccc agcattcggg catgccgcac agttcttcgg
     2941 agccgggcgt gatgctgtcc aggtcgcgca cgtcgaggcg gtattcgccc ttgccggcgc
     3001 gcaggcgggc cagcgtgatc agctcgctca ggttgccata gccctggcgc gtctgcgcca
     3061 gcagcaccag ccggcagccg ggcgtgtcgc cggccggctg cacgtcgaac tcgctgccga
     3121 cgatgaagcg gaagcttttc ttctgctcgt cgctccagcc cttgatcgcg gtatgcgccc
     3181 gcaccacgcc ggccaccgag cattcgtcgg ttaggcctaa gcccgcatag cccagctcca
     3241 gcgcgcgggc gaccagctct tcgggatggg aggcgccggt gaggaagctg aagttcgagc
     3301 ggcagtgcaa ttccgcgtag ccgggcaagc ccggcgcaag cggcaacaag gtgccaggag
     3361 gccgttcggg ccgcagtttc ggcgggaagc gttgctcggg catggcatcg atccatccat
     3421 cattcaggca aacaagccat gcaggaacca ggcgcgttcc ccgccatggc cgcgcctttc
     3481 gcaatagacc cagcgcagac ggtgatcgct gccctcggcg acgtggtagt cgcggcacac
     3541 cagcttgccg tcgaaccagc cggcctcgat gcgctcggcc cgcgtcagca gattcagcac
     3601 cgtgctgccg tgcaccgggc gctccccctg ttcgcgcagg cgcagcggaa ccggcagcag
     3661 ccaggcgggg cgcggcagct gcggtacatc ggcgcactgc tgggccaatc gatcgggttg
     3721 gcgcgacaac agcacatgcg ccagctccgg tctatggtcg gcacgcaggg ccgggtgctg
     3781 cacctgctcg gcgcccaggc gggcgcgcag gcggtccagc aaggccagga aatcctgcgc
     3841 ctcgcgaccg ggatcgggca gcagttggcc ttcggcgccg gggcagggcg cggcttcttc
     3901 gagcaccagg cgcagccagt acaccggagc ctggagcgtg aggcgatgca ggcgttcgcg
     3961 cagcagtgtc tgcagctgct gcgggtcacg cgtggcctgg cccagctcga tcctgagcag
     4021 ggtgtccggc gcggcgtggc ggccgcgctc atggcgcagc acgagccgca cccgcgtggc
     4081 ggccagccat tgccgtgaca gccagccgca cagaggctgc agcaggcgct gggcagcgaa
     4141 aaccagcgcc tcggcctggt cgacgcggtg catcagctcc agctcggcgt ggaattcagg
     4201 cggagcggcg aaccaatcgc gcgggtccgg cgcgtcgcca taggcacgat ccagcgcctg
     4261 caacagggcc atgccgccgc ggcgctgcag gcccgcgcgc ggcagggccc gcacatcgcc
     4321 gagacggcgg cagcctatgg cctgcagcag ctcggccagg cgcggcggct g
//
LOCUS       DASUCY010000623         2130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_801372, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000623 DASUCY010000000
VERSION     DASUCY010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801372"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VFV25_12370"
     CDS             complement(<1..342)
                     /locus_tag="VFV25_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEY1394161.1"
                     /translation="MNSNNRNLRNIGVIAHVDAGKTTTTERILYYTGENYRIGEVDDG
                     NTRMDFDPQERQRGITINSAAITVHWKGAQINLIDTPGHIDFNIEVNRSLRVLDGAVV
                     VFDGVAGVEPQT"
     gene            complement(737..1225)
                     /locus_tag="VFV25_12375"
     CDS             complement(737..1225)
                     /locus_tag="VFV25_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1394162.1"
                     /translation="MSRRRIHRFCTLSFTLSVGTLGGALLLALPAITHAQRPPVEIST
                     QTGLPRHDVHAVCPGIDQSLQQSLDYAWQRVRQPGLVSMQFRLKGQQITEVTPTGGTF
                     DYFRYVRRAVGKLACNSDSDDEQLFAFDVRFVEVYADSGAPAMALRIEEPESMVERVA
                     SH"
     gene            1414..>2130
                     /locus_tag="VFV25_12380"
     CDS             1414..>2130
                     /locus_tag="VFV25_12380"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1394163.1"
                     /translation="MSHIRVLADRRHFLAFTSGCLAVTAGVVLHLPMFLMGRDNGFRL
                     ADMPMDVDMWWGMLLIVAGIALAAYGLLPRRPAHEPPHHLRISAPEDAPLTWAHWQLM
                     LVLVVALVIDVMKPASLGFVLPGMKSEYAVSGTMVAWLPLSALTGTVIGSMLWGLLAD
                     VYGRRASILLSAVMFVGTSICGAMPSFWWNVGMCALMGAAAGGMLPVTYALLAETMPS
                     RHRGWALVLVGGLGSVGGYLA"
BASE COUNT          331 a          675 c          729 g          395 t
ORIGIN      
        1 ggtctgcggc tccacgccgg ccacgccgtc gaagaccacg accgcgccgt cgagcacgcg
       61 cagcgagcgg ttcacctcga tgttgaagtc gatgtggccg ggcgtgtcga tcaggttgat
      121 ctgcgcgccc ttccaatgca ccgtaatcgc cgcgctgttg atcgtaatgc cccgctggcg
      181 ctcctgcggg tcgaagtcca tgcgcgtgtt gccgtcatcc acctcgccga tgcgatagtt
      241 ctcgcccgtg tagtacagga tgcgttcggt ggtcgtggtc ttgccggcgt cgacatgagc
      301 gatgacgccg atgttgcgga ggtttctgtt gttcgagttc atggcgaatc tttcgggcac
      361 gacgccgctc cttgcgcgcg tcgatgcagc ctggtggcag ggcaaacccc aggcgcgatt
      421 cgcctgaggg ctagcggcgg atatgcggtc ggcggccgga gcccgggcgc acgcggtcca
      481 cgctccggcg agcggaaatc gtggcgatca acttgtcgta ggtgcgcata gaaatctcag
      541 aaacaaaaac cccggaggac aggacctacc ggggtttatg ggccggaagg cgggcagcct
      601 tccgaaacgc gatcgaaagg cgcagtcagg tggcgaaagt cgccttgatc acattggtga
      661 gcttcatctt gaggcaaatt gtctcaagcc aagctgcgcc atgccaggag tgtgggttct
      721 gtgtcgccgc caccgttcag tgcgacgcga cccgctccac catcgactcc ggctcctcga
      781 tgcgcagggc catggcggga gcgccactgt cggcatagac ctcgacgaag cggacatcga
      841 aggcgaacag ctgctcgtca tcgctatcgc tattgcaggc cagcttgccg accgcccgcc
      901 gcacgtaacg gaagtagtcg aacgtgccac cagtcggcgt cacctcggtg atctgctgcc
      961 ccttgagcct gaactgcatg ctgaccaagc caggctggcg cacgcgctgc caggcgtagt
     1021 ccagcgactg ctgcaggctc tggtcgatgc cggggcagac ggcatggacg tcgtggcgcg
     1081 gcaacccggt ctgcgttgag atctcgaccg gcggccgttg cgcgtgcgtg atcgcgggca
     1141 gcgccagcag caatgcgccg cccagtgttc cgactgacag cgtgaaagac agcgtgcaaa
     1201 agcggtgaat tcttcttcgc gacatgggca gcctccttgg gtgttcgggc cgtgtgcgcc
     1261 aggagcggcc tgcgtggccc agcagcaagg ctaggcaccg ggaggcggca caggtagcgc
     1321 ggcgcgacga actgcagaaa tggccgacga agcgacaaaa ggcgagaaaa tccgggcctt
     1381 ccttccagcc ttccgcggca cctgccgctt gcgatgagcc atatccgcgt ccttgccgac
     1441 cgccggcatt tcctcgcctt cacatccggc tgcctggccg tgacggccgg cgtcgtgctg
     1501 catctgccga tgttcctgat gggccgcgac aacggctttc gcctggccga catgccgatg
     1561 gacgtcgaca tgtggtgggg catgctgctc atcgtggccg gcatcgcgct ggccgcttac
     1621 ggcctgttgc cgcgccgccc cgcccacgag ccgcctcacc acctgaggat cagcgcgccg
     1681 gaagacgcgc cgctcacctg ggcgcattgg cagctgatgc tggtgctggt ggtggcgctg
     1741 gtgatcgacg tgatgaagcc ggccagcctg ggtttcgtgc tgccggggat gaaatccgaa
     1801 tacgcggtgt ctggaacgat ggtggcctgg ctgcccttgt ccgcgctgac cggcaccgtg
     1861 atcggctcca tgctctgggg cctgctggcc gacgtctacg gccggcgcgc gtccatcctg
     1921 ttgtcggcgg tgatgttcgt cggcacctcg atctgcggcg ccatgccttc gttctggtgg
     1981 aacgtcggta tgtgcgctct gatgggggcc gcggcgggcg gcatgctgcc ggtcacatat
     2041 gccctgctgg ccgaaaccat gccctcgcgc caccgcggct gggcgctggt gctggtgggc
     2101 gggctgggct cggtgggggg ctatctggcg
//
LOCUS       DASUCY010000624         4744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_126598, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000624 DASUCY010000000
VERSION     DASUCY010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126598"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VFV25_12385"
     CDS             complement(<1..576)
                     /locus_tag="VFV25_12385"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEY1394164.1"
                     /translation="MGPGAVKAKAWLRKLRWWIALALAIVAWQAWSRATAPAPPSPPL
                     GEVKLADIVQTVQAAGTLQAKTRVDVGAQVSGQVKDIPVVLGHAVRKGDLLVLLDPEL
                     AQSDVARAEASVAQQRAQLEARRVDLEAARRELERQRRLLKGEATAAAEAEKAENDVA
                     KLEADVRGQDANLAQLQAQLERSKLNLAYTRI"
     gene            899..1108
                     /locus_tag="VFV25_12390"
     CDS             899..1108
                     /locus_tag="VFV25_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011546457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEY1394165.1"
                     /translation="MLCGSSHSLDLSVSEAAQQLGVSRVALSRVINGRAGISPEMALR
                     LQAWLGVERGGDARVWLAQQIAYDL"
     gene            complement(1242..1329)
                     /locus_tag="VFV25_12395"
     tRNA            complement(1242..1329)
                     /locus_tag="VFV25_12395"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1293..1295),aa:Ser,seq:tga)
     gene            complement(1362..2186)
                     /locus_tag="VFV25_12400"
     CDS             complement(1362..2186)
                     /locus_tag="VFV25_12400"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HEY1394166.1"
                     /translation="MNYLIGDIQGCCDALDRLLAEIAFSPSRDRIYVLGDMVNRGPAS
                     LKVLKLLRGFDDAAVCLLGNHDLHLLAVAAGVRKPHRKDTTEDILTSPEREQWLAWLR
                     RRRLAVHEHGWLMLHAGVVPQWDLAQTLSLAAEVEALLQGPELGNFINRMYGNEPARW
                     SEDLAGIDRWRFIVNVLTRTRFCSADGVLEFDTKEGADQAPSGYLPWFEVPGRKTADT
                     PIAFGHWSSLGLVNRPKLLAIDTGCVWGGQLTAVRVDGGEQREVIQVECHQAQGHG"
     gene            2309..3607
                     /locus_tag="VFV25_12405"
     CDS             2309..3607
                     /locus_tag="VFV25_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEY1394167.1"
                     /translation="MDVALLIFLILLNGAFAMSEMALAASRKARLQVMVESGEAGASA
                     AMELHDNPTQFLSTVQIGITSIGVLNGIIGEAAFTAPLAAWLKAALHVPDRAAEIGSL
                     VLVVVVITFLTIIFGELVPKRIGQMYPEAVAGVVARPMQWLSRLARPFVKLLAASTVA
                     TLKLLGVVENPSRAVTEEEIAASLEEGLDAGVIEAHEHQMVRNVFRLDERPIGSMMIP
                     RDDIAWLDLNAPLEASIKRIVEHDHSRYPVCRGGLDDVVGVLAAQHLLQQLTQGRSLS
                     LEERLQPPVFVPETLTGMELLEQFRGSSAQMVFVVDEYGEVQGMITVRDVLEAITGEF
                     TTPSAEDAWAVQREDGSWLIDGLIPVTELKDRLELKELPDEDRGRYNTLAGMVMLLLG
                     RLPRTTDVVEWGGWRFEVVDLDGKRVDKLLVSRLPSGDIE"
     gene            3604..4389
                     /locus_tag="VFV25_12410"
     CDS             3604..4389
                     /locus_tag="VFV25_12410"
                     /inference="COORDINATES: protein motif:HMM:NF018928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapC family protein"
                     /protein_id="HEY1394168.1"
                     /translation="MSFPSALYKNPVLLDRNEHRGKRIKQVADFSAAREMNAVFVNVV
                     EFADCAKEFPVAFLASPGPDADSKPVVNPVVLLGLREAENLFVEGTRWDARYIPAFVR
                     RYPLAYAKSAEDRVSVVVDAAWPGFNDREGELLVDDKGEATEYLKKYMAFLDEFEREA
                     GRTRTFCEQLVELDLLRGAEVNGQLAEGVPLKVEGFFAVDEQRLLALPDAKLLELVKS
                     GALALIHAHMLSMGNVQRLFERLAPRVQDLAAAAPAAATPAAA"
     gene            4617..>4744
                     /locus_tag="VFV25_12415"
     CDS             4617..>4744
                     /locus_tag="VFV25_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEY1394169.1"
                     /translation="MASSRILVAQGGGPTAVINQSLVGAVLEARRFAGIERVYGAR"
BASE COUNT          729 a         1523 c         1649 g          843 t
ORIGIN      
        1 gatgcgggtg taggcaaggt tgagcttgct gcgttcgagc tgggcctgca gctgggccag
       61 gttggcgtcc tggccgcgca catcggcctc gagcttggcg acgtcgttct cggccttctc
      121 ggcttcggcg gcggcggtgg cctcaccctt gagcaggcgg cgctgacgct caagttcgcg
      181 acgcgcggcc tcgagatcga cccgacgcgc ctcgagctgc gcgcgctgct gcgccaccga
      241 ggcctcggcg cgagccacat cgctctgcgc gagttcgggg tcgagcaaca ccagcaggtc
      301 gcccttcctg accgcatggc ccagcaccac cgggatgtcc ttgacctggc cgctgacctg
      361 ggcgcccacg tccacgcggg tcttggcttg cagggtaccg gcggcctgta cggtctggac
      421 gatgtcggcc agcttgacct cgccgagggg cggcgatggg ggcgctgggg cggtggcgcg
      481 gctccaggcc tgccaggcga cgatcgcaag cgcgagcgcg atccaccaac gcagcttgcg
      541 cagccaagcc ttggccttta cagcacctgg acccattccg tttcctcctg attgttgttg
      601 ggggagcaag gcgagggagt gccgggcgcg agcatcgctg cgcttggctc gccccttccg
      661 tcagtccggg gacagcaggg gctcgcatct caaggcctac ctggcggtga ttgagaagtt
      721 ctgcgagtag aagttcatgc cgccagccga cgaggtgatc tcgaagccgt actgcacttc
      781 gcccatcgtc acgtcgctca tcgatcccag gctggtggag tctggggctg cctactgtca
      841 ctgtgcttag gtgtttcctc gcgatttact tcgttcgtcg atcgaactaa tatccgcaat
      901 gctctgcggg tcatcgcatt cactcgatct gtccgtcagc gaagcggcgc agcagctcgg
      961 tgtgtcgcgg gtggcgctgt cccgcgtgat caacggccgt gctgggattt cacccgagat
     1021 ggcgctgcgc cttcaggctt ggctgggggt cgagcgcggc ggtgatgccc gtgtctggct
     1081 tgcacagcag atcgcctacg acctgtagca agccgagcaa gggctcaaga aggcacccct
     1141 gcgtgtacgg cgcgcgcctg cgcatgcttg agcggcgggg cgtggttttc ctgtcccgcc
     1201 ctcagaagat atgttcgcga ggccggcatt catgccggtc ctggcggaag aggtgggatt
     1261 cgaacccacg aacggttgcc cgttgccggt tttcaagacc ggtgcaatcg accactctgc
     1321 cactcttcca gaggggagag gcattgtatt cggccgcccc ttcagccatg cccctgcgcc
     1381 tggtggcatt ccacctggat cacctcgcgc tgctcgccgc cgtccacgcg cacggcggtg
     1441 agctggccgc cccagacgca gccggtatcg atggcgagca gcttgggccg gttgacgagg
     1501 ccgagcgagg accagtggcc gaaggcgatg ggcgtgtccg ccgtcttgcg gcccggcact
     1561 tcgaaccagg gcaggtagcc ggacggggcc tggtcggcac cttccttggt gtcgaactcc
     1621 agcacgccgt cggcgctgca gaagcgggtg cgcgtgagca cgttgacgat gaagcgccag
     1681 cggtcgatgc cggcgaggtc ctcgctccag cgcgcgggct cgttgccgta catccggttg
     1741 atgaagttgc ccagttcggg gccctgtagc agggcctcga cttcggcggc caggctcagg
     1801 gtctgggcca ggtcccattg cggcaccacg ccggcgtgca gcatcagcca gccgtgctcg
     1861 tgcacggcca ggcggcgccg gcgcagccag gcgagccatt gctcgcgttc tggcgaggtg
     1921 aggatgtcct cggtggtgtc cttgcggtgc ggcttgcgca cgccggcggc cacggcgagc
     1981 aggtgcaggt cgtggttgcc gagcaggcag accgcggcat cgtcgaagcc gcgcagcagc
     2041 ttgagcacct tcagcgaggc ggggccgcgg ttgaccatgt cgcccagcac gtagatgcgg
     2101 tcgcgcgagg gcgagaaggc gatttcggcc agcaggcgat ccagcgcatc gcagcagcct
     2161 tggatgtcgc cgatcagata gttcatcgcc agggagaatc cgtgagattt gtggagcggt
     2221 tgagccggcc gattctgcta cgcttggcgc cctttgaaat cacgctaggt tcggcgccgc
     2281 ggctcccgcc tgcgagctcg cctcggtaat ggacgttgcg ctcctgattt tcctgatcct
     2341 cctcaacggc gccttcgcga tgtcggagat ggcgctggcc gccagccgca aggcgcggct
     2401 gcaggtgatg gtggaaagcg gcgaggcggg cgccagcgcg gccatggagc tgcacgacaa
     2461 cccgacgcag ttcctgtcca ccgtgcagat cggcatcacg tccatcgggg tgctcaacgg
     2521 catcatcggc gaggcggcct tcaccgcgcc gctggcagcc tggctcaagg cggcgctgca
     2581 cgtgcccgac cgcgcggccg agatcggctc gctggtgctg gtggtggtgg tcatcacttt
     2641 cctgaccatc atcttcggcg aactcgtgcc caagcgcatc ggccagatgt atccggaagc
     2701 cgtggccggc gtcgtggccc ggcccatgca gtggctgtcg cgcctggcgc ggcccttcgt
     2761 gaagctgctg gccgccagca ccgtggcgac gctgaagctg ctgggcgtgg tcgagaaccc
     2821 cagccgcgcc gtcaccgaag aggaaatcgc cgccagcctg gaagaaggcc tggacgccgg
     2881 cgtgatcgag gctcacgagc accagatggt gcgcaatgtg ttccgtctgg acgagcgccc
     2941 gatcggctcg atgatgatcc cgcgcgacga catcgcctgg ctcgatctga atgcgccgct
     3001 ggaggcatct atcaagcgaa tcgtcgagca tgaccactcg cgctacccgg tgtgccgcgg
     3061 cgggctggac gacgtggtgg gcgtgctggc ggcccagcac ctgctgcagc agctgaccca
     3121 ggggcgcagc ctgtcgctgg aggagcggct gcagccgccg gtcttcgtgc cggagacgct
     3181 caccggcatg gagctgctgg agcagttccg cggctccagc gcgcagatgg tctttgtggt
     3241 cgacgaatac ggcgaagtcc agggcatgat cacggtgcgc gacgtgctgg aggccatcac
     3301 cggagaattc acgacgccca gcgccgagga cgcctgggcc gtgcagcgcg aggacggctc
     3361 ctggctgatc gacgggctga tcccggtcac cgagctgaag gatcgccttg aattgaaaga
     3421 actgcccgac gaagatcgtg ggcgctacaa caccctggcc ggcatggtga tgctgttgct
     3481 gggccggctg cctcgcacca ccgatgtggt ggagtggggc ggctggcgct tcgaagtcgt
     3541 cgacctcgac ggcaagcgtg tcgataagct gctggtcagc aggcttccct ctggagatat
     3601 tgaatgagct ttccttccgc cctctacaag aaccccgtgc tgctggaccg caacgagcat
     3661 cgcggcaagc gcatcaagca ggttgccgat ttctcggcgg cgcgcgagat gaacgcggtc
     3721 ttcgtcaacg tggtcgagtt cgccgactgc gcgaaggaat ttccggtcgc cttcctcgct
     3781 tcgccggggc cggatgccga tagcaagccg gtggtcaatc ccgtggtgct gctgggcctg
     3841 cgcgaggccg agaacctctt cgtcgagggc acgcgctggg acgcccgcta tatccccgcc
     3901 ttcgtccgcc gctatccgct ggcctatgcc aagagcgctg aagaccgcgt cagtgtcgtg
     3961 gtcgatgccg cctggcccgg cttcaacgac cgcgaaggcg agctgctggt cgatgacaag
     4021 ggcgaggcca ccgagtacct caagaagtac atggccttcc tcgacgagtt cgagcgcgag
     4081 gcaggccgca cccgcacctt ctgcgagcag ctggtggagc tggacttgct gcgcggcgcc
     4141 gaggtcaacg gccagttggc cgaaggcgtg ccgctcaagg tcgagggctt cttcgccgtc
     4201 gacgagcagc ggctgctggc cctgccggac gccaagctgc tggagctggt gaagagcggc
     4261 gcgctggcgc tgatccacgc ccacatgctc tccatgggca atgtgcagcg cctgttcgaa
     4321 cggctcgcgc cgcgggtgca ggatctggcg gccgccgcac cggccgcggc tacacctgcc
     4381 gcagcctgat ttgctacata ttttgtagga gcaactggcg tatttattcc ggagattgac
     4441 ggaaatctct tgaatttctg ctcagccggg atctgaacgc ccagccgggc agacgccgcc
     4501 gagccagcca gaccgctgat tcggcggcgt catcacttgc cgggctcgct tctggcacca
     4561 taagctgcga gcagcgggcg ctttgtgctc ggctcgcaac ggtcaaggag gcggcaatgg
     4621 catccagcag gatcctggtg gctcagggcg gcggcccgac ggcggtgatc aaccaatcgc
     4681 tggtcggcgc ggtgctggag gcgcggcgct tcgccggcat cgagcgcgtc tacggcgcgc
     4741 ggca
//
LOCUS       DASUCY010000625         2588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_785454, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000625 DASUCY010000000
VERSION     DASUCY010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785454"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1330
                     /locus_tag="VFV25_12420"
     CDS             <1..1330
                     /locus_tag="VFV25_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEY1394170.1"
                     /translation="QRCSTAQAVREQHGRDESPFDVPPPDAVVYCESNEDVAFVVALA
                     SEHAVPVIPYGAGSSLEGHLLAVQGGVSIDLSRMNAVLRVNAEGLTVSVQAGVTRKQL
                     NEAVKHEGLFFPIDPGADASIGGMCATRASGTNAVRYGTMRENVLGLTVVTASGEIIH
                     TGTRARKSSAGYDLTRLFVGSEGTLGVMTEITLKLYPLPEAVSAAICFFPSVDAAVRT
                     TIQIIQLGVPIARCELLDAHAVRAVNAHDKLGLRESPMLLMEFHGSPAGVKEQAETVQ
                     EIAREQGGEDFAWATTPEERTRLWTARHHAYLSALQTKPGCRCVTTDTCVPISRLAEC
                     VGETVAEADAAGLPYFIVGHVGDGNFHVGYLIDPQLPAERELAESLNQRLVARALRME
                     GTCTGEHGIGLHKMDFLLAEAGAGAVGLMRSVKRSLDPKNIMNPGKIFTF"
     gene            1398..2453
                     /locus_tag="VFV25_12425"
     CDS             1398..2453
                     /locus_tag="VFV25_12425"
                     /inference="COORDINATES: protein motif:HMM:NF015724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HEY1394171.1"
                     /translation="MQQKLSFAAARRPGAISLLLFLLGAAALAGLSACATSQETTIGG
                     KPDVFIANGDGGEISRCRNRIAAVAISESDSNAQALSSAGLPRSMAPLVRQLLMQTRC
                     FAVVDRSSTFAALENEVKIREQQGQARAAQMAAMTPADYLIRAEIVFVEQTDGRKGTI
                     GALFGNVIGGLSPEMRKREALVMLSAVDTRTSEIVASSFGRGTSETSTMGGLVLGGGL
                     IAIEGGWLDTPQAKPVAAALVDSWNQLLPRLVAAHEKNAATAAAARKDKPQAAAAAQP
                     TASAALPAASAPATAASAPAAPAAPVIPAAPAQPASGAAMAITITPLPPLQPASAPEP
                     AASAVVPPVASAASAPR"
BASE COUNT          411 a          903 c          892 g          382 t
ORIGIN      
        1 gcagcgctgt tccactgcac aggccgtgcg cgagcagcat gggcgcgacg agtcgccctt
       61 cgatgtgccg ccgcccgatg cggtggtgta ttgcgagagc aatgaggatg tggccttcgt
      121 ggtcgcgctg gccagcgagc atgcggtgcc ggtgatccct tatggcgcag gctcgtcgct
      181 cgaaggccat ctgctcgcgg tgcaaggcgg cgtgagcatc gacctgtcgc gcatgaacgc
      241 agtgttgcgc gtcaacgccg agggtctgac cgtgtccgtg caggccggcg tgacacgcaa
      301 gcagctcaac gaggcggtca agcacgaagg gctgttcttc cccatcgacc ccggcgccga
      361 tgcgtcgatc ggcggcatgt gcgcgacgcg cgccagcggc acgaacgcgg tgcgctacgg
      421 gacgatgcgt gagaacgtgc tggggctcac ggtcgtcacc gccagcggcg agatcatcca
      481 caccggcacg cgggcgcgca agtccagcgc gggctatgac ctgacgcggc tcttcgtcgg
      541 cagtgaaggc acgctgggcg tgatgaccga gatcacgctc aagctctatc cgctgcccga
      601 ggcggtgtcg gccgcgatct gcttctttcc cagcgtcgac gcggcggtac ggaccacgat
      661 acagatcatc cagctgggcg tgccgatcgc gcgctgcgag ctgctcgacg ctcacgcggt
      721 gcgcgccgtc aatgcacacg acaagctggg cctgcgcgag tcgccgatgc tgctgatgga
      781 gttccacggc agcccggctg gggtcaagga gcaggccgag accgtgcagg agatcgcccg
      841 cgagcaaggc ggggaggact tcgcctgggc caccacgccc gaggagcgca cgaggctgtg
      901 gacggcgcgt caccatgcct atttgtcggc gctgcagacc aagcctggct gccgctgcgt
      961 caccaccgac acctgcgtgc cgatctcgcg cctggccgag tgcgtgggcg agacggtggc
     1021 cgaggccgat gcggccggcc tgccctattt catcgtcggc cacgtgggcg acggcaactt
     1081 ccacgtgggc tatctgatcg acccgcagct gcctgccgag cgcgagctgg ccgagagcct
     1141 gaaccagcgc ctcgttgcgc gtgcgctgcg catggagggc acctgcactg gcgagcacgg
     1201 catcggcctg cacaagatgg acttcctgct ggccgaagcg ggtgccggcg cggtggggtt
     1261 gatgcgcagc gtgaaacgct cgctggaccc caagaacatc atgaacccgg gcaagatctt
     1321 cacattctga gaatccaccg gctgcgcgcc aacgtttggg aacaggagcc cagggctcct
     1381 gagttgtaat agaaggcatg cagcagaaac tttcctttgc cgccgcgcgc cgcccaggcg
     1441 cgatttccct cctcctgttc ctcctcggcg ccgccgccct ggccgggttg tccgcctgcg
     1501 ccacgagcca ggagaccacg ataggcggca agcccgacgt cttcatcgcc aacggcgacg
     1561 gcggcgaaat ctcgcgctgc cgcaaccgca ttgccgcggt ggcgatcagc gagtcggaca
     1621 gcaatgccca ggcgctcagc tcggccggcc tgccgcgctc gatggcgccg ctggtgcgcc
     1681 agctgctgat gcaaacgcgc tgcttcgcgg tggtggaccg cagctcgacc ttcgcggcgc
     1741 tggagaacga ggtcaagatc cgcgagcagc agggccaagc gcgcgcggcc cagatggcag
     1801 cgatgacgcc ggccgactac ctgatccgcg ccgagatcgt cttcgtcgag cagaccgacg
     1861 gccgcaaggg cacgataggc gcgctgttcg gcaacgtgat cggcggcctc agccccgaga
     1921 tgcgcaagcg cgaggccctg gtgatgctga gcgccgtcga cacgcgcacc agcgagatcg
     1981 tcgcctcctc cttcggccgc gggaccagcg aaaccagcac catgggcggc ctggtgctgg
     2041 gcggcgggct gatcgcgatc gaaggcggct ggctggacac gccccaggcc aagccggtgg
     2101 ccgcggccct ggtcgattcc tggaaccagc tgctgccgcg gctggtggcg gcccacgaga
     2161 agaatgcagc cacggcggcg gcagcgcgca aggacaagcc gcaagcggca gccgcggccc
     2221 aaccgacggc atcggcggct ctgccggctg cctcggcgcc ggcgacggcg gcatccgctc
     2281 ccgcggctcc cgccgctccc gtcattcccg ctgctccggc tcagccggct tcgggggcgg
     2341 ccatggccat cacgatcacc cccttgccgc cgctgcagcc ggcctcggcc cccgagccgg
     2401 ctgcttcggc cgtcgtgccg cccgtggcct ccgcggcctc cgccccgcgc tgaagcggcc
     2461 tggagaggcg gatgcaagcg gcaacagccg tttgccaacg ctcactgata cgccggcgtc
     2521 ttgggcgtat acctgagact ccgctgagct ctctccctcg gacaaaccaa aatgaacgcc
     2581 gccgcctt
//
LOCUS       DASUCY010000626         2863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1411960, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000626 DASUCY010000000
VERSION     DASUCY010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1411960"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VFV25_12430"
     CDS             <1..292
                     /locus_tag="VFV25_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HEY1394172.1"
                     /translation="KDGESAIFTLADGSHRVAGDAPIAEVNGAFNTALPDQDFDTIGG
                     LVAHELGRVPRRSEAVEVGGLRFVVMLTRGGAVRWFKVAPAHEASADTAAAA"
     gene            289..1857
                     /gene="lnt"
                     /locus_tag="VFV25_12435"
     CDS             289..1857
                     /gene="lnt"
                     /locus_tag="VFV25_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEY1394173.1"
                     /translation="MKPGLASTLGRLPGYLAPAWAELGQGGALGLLHALAFARPGLWL
                     LELAALALLAWRAGRTSAPGPGFWLGLGFGTGWLLGGVWWLFISMYRYGGLPAWLSAL
                     AVLALCGFLALYYAAALALFVRWRRGRPWADASLFAALWLLAELARGLIFTGFPWVAS
                     GYAHVEGPLGALAPWIGVYGIAACAPWLAAFVVAAPRAGKPVWRAPLLVLAVLALLYA
                     LPVPDFTQATGTLRLSLLQGNVPQDEKFAEEHLPQTLAWDARSLVTARGDLVVGPETV
                     IPLLPDQLPDGYWDALTAHFREGHRAALIGVPLGSFEKGYTNSAAGLSLDTAPLLGGF
                     YRYDKHHLVPFGEFIPLGFHWFVDMMNMPLGDFNRGPLNPLSFEVKGERIAPNICYED
                     LFGEELATRFVDAAKAPTMFANIGNIGWFGDTVAVSQHLEISRMRALEFQRPMLRATN
                     TGATAVIDHRGRVTARLEPFTQGVLEASVQGRSGITPYAWWVGRLGLWPLILAALACV
                     GWAALQPGPGRAAP"
     gene            1916..2821
                     /gene="glyQ"
                     /locus_tag="VFV25_12440"
     CDS             1916..2821
                     /gene="glyQ"
                     /locus_tag="VFV25_12440"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEY1394174.1"
                     /translation="MLSFQQIIQRLQQYWSDQGCALLQPYDMEVGAGTSHTATFLRAI
                     GPEPWKAAYVQPSRRPKDGRYGENPNRLQHYYQYQVVLKPAPSNILELYLGSLQALGF
                     DLKKNDIRFVEDDWENPTLGCWGLGWEVWLNGMEVTQFTYFQQVGGIECKPITGEITY
                     GLERLAMYLQGVDNVYNLQWTKGLTYGDVYKQNEVEQSAYNFEHSDVEFLLGAFGAHE
                     KQAKHLMEQQLALPAYEQVLKAAHTFNLLDARGAISVTERAAYIGRIRNLARAVAQSY
                     YDSRERLDFPMAPREWVAEMQKKAA"
BASE COUNT          423 a          990 c          989 g          461 t
ORIGIN      
        1 gaaggacggc gaaagcgcca tcttcacgct ggccgacggc agccatcgcg tggccggcga
       61 cgcgcccatc gcagaggtca acggcgcctt caacacggcc ctgcccgacc aggatttcga
      121 caccatcggc ggcctggtcg cccatgagct gggccgcgtg ccgcggcgca gcgaggccgt
      181 cgaggtcggc ggcctgcgct tcgtcgtgat gctgacgcgc ggcggcgccg tgcgctggtt
      241 caaggtggcg ccggcccacg aagccagcgc cgacacggcg gccgcggctt gaagcccggt
      301 ctcgccagca cgctcggccg cctgcccgga tacctggcgc cggcctgggc cgagctaggc
      361 cagggcggcg ctctggggct gttgcatgct ctggcattcg cgcgacccgg gctttggctg
      421 ctggagctgg cggccttggc gctgctggcc tggcgcgccg gccggacgag cgctcctggg
      481 ccgggcttct ggctgggcct gggcttcggc accggctggc tgctgggcgg cgtgtggtgg
      541 ctcttcatca gcatgtaccg ctacggcggc ctgccggcct ggctgtccgc gctggcggtg
      601 ctggccttgt gcggcttcct ggccctctat tacgccgcgg ccctggcgct cttcgtgcgc
      661 tggcgccgcg ggcggccctg ggccgatgcg agcctgttcg ccgcgctatg gctgctggcc
      721 gagctggcgc gcggcctcat cttcaccggc tttccctggg tggcgagcgg ctatgcccat
      781 gtcgaggggc cgctgggcgc gctggcgccc tggatcgggg tctatggcat cgcggcctgt
      841 gcgccctggc tggcggcctt cgtcgtggcc gcgccgcgcg ccggcaagcc ggtctggcgg
      901 gcgccgctgc tggtgctggc ggtgctcgcg ctcctgtacg cgctcccagt gccggacttc
      961 acgcaggcca ccggcacgct caggctcagc ctgctgcaag gcaatgtgcc gcaggacgag
     1021 aaattcgccg aggaacacct gccccagacg ctggcctggg atgcgcgcag cctggtgacg
     1081 gcgcgcggcg atctcgtcgt ggggccggaa accgtgatcc cgctgctgcc cgatcaactg
     1141 cccgatggct actgggacgc gctgaccgcg catttccgcg agggccatcg cgcggccctg
     1201 atcggcgtgc cgctgggcag cttcgagaag ggttacacca attcggccgc cggcctgtcg
     1261 ctggatacgg cccccttgct gggcggcttc tatcgctacg acaagcatca cctggtgccc
     1321 ttcggcgagt tcattccgct gggcttccac tggttcgtcg acatgatgaa catgccgctg
     1381 ggcgacttca accgcggccc gctgaacccg ctgtccttcg aagtgaaggg cgagcgcatc
     1441 gcgcccaaca tctgctacga agacctgttc ggtgaggaac tggcgacgcg cttcgtcgac
     1501 gcggcgaagg cgccgaccat gttcgccaac atcggcaaca tcggttggtt cggcgacacg
     1561 gtggcggtga gccagcacct ggagatctcg cgcatgcgcg cgctggagtt ccagcgcccc
     1621 atgctgcgcg ccaccaacac cggcgccacc gcggtgatcg accaccgggg ccgcgtgacg
     1681 gcgcggctcg agcccttcac ccagggcgtg ctcgaagcga gcgtgcaggg gcgcagcggc
     1741 atcacgcctt atgcctggtg ggtaggccgc ctggggctgt ggccgctgat cctggccgcg
     1801 ctggcctgcg tgggctgggc ggcactgcag cctggtccgg ggcgggccgc cccgtaaaat
     1861 caagcgttgc gccgcgccgc ccgcgcgtgg cgccctcaca ctagacaccc cacaaatgct
     1921 gagcttccag caaatcatcc agcgcttgca gcaatactgg tccgaccagg gctgcgccct
     1981 gctgcaaccc tatgacatgg aagtgggcgc cggcaccagc cacaccgcga ccttcctgcg
     2041 cgcgatcggc cccgagccct ggaaggccgc ctacgtgcag cccagtcgtc gccccaagga
     2101 cggccgctac ggcgagaacc cgaaccgact gcagcactac taccagtacc aggtggtgct
     2161 caagcccgcg cccagcaata tcctggagct ctacctcggc tcgctgcagg ccctgggctt
     2221 cgacctcaag aagaacgaca tccgcttcgt cgaggacgat tgggagaacc cgacgctcgg
     2281 ctgctggggc ctgggctggg aggtctggct caacggcatg gaggtgacgc agttcaccta
     2341 cttccagcag gtgggcggca tcgagtgcaa gcccatcacc ggcgagatca cctacggcct
     2401 ggagcgcctg gcgatgtatc tgcagggcgt ggacaacgtc tacaacctgc agtggactaa
     2461 agggctgact tatggagacg tctacaagca gaacgaggtc gagcagtcgg cctacaactt
     2521 cgagcacagc gacgtggagt tcctgctggg cgcgttcggc gcccacgaga agcaggccaa
     2581 gcacctgatg gagcagcagc tcgcgctgcc ggcctacgag caggtgctca aggccgccca
     2641 caccttcaac ctgctcgatg cgcgcggcgc gatcagcgtc accgagcgcg cggcgtacat
     2701 cggccgcatc cgcaacctcg cgcgtgccgt cgcgcagagc tactacgaca gccgcgagcg
     2761 cctggatttt ccgatggcgc cgcgcgagtg ggtggccgag atgcagaaga aggcggcgtg
     2821 acgatgaaaa accttctcgt cgaactgttt gtcgaggaac tgc
//
LOCUS       DASUCY010000627         3018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1106724, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000627 DASUCY010000000
VERSION     DASUCY010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106724"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VFV25_12445"
     CDS             <1..243
                     /locus_tag="VFV25_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEY1394175.1"
                     /translation="GVQKLKDATPAGVLLGSGKLATELDRLDLIDEYKFLVHPRIAGH
                     GPTLYESGLPSTRRLELISAKPLRSGAVAMHYRRAR"
     gene            complement(465..821)
                     /locus_tag="VFV25_12450"
     CDS             complement(465..821)
                     /locus_tag="VFV25_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HEY1394176.1"
                     /translation="MTSNIKLYGISNCDTVKKARAWLQAEGVAHDFHDFKKLGLAAER
                     LDAWIATLGWERLLNRQGTTWRKLDPEQQAAVVDAASAKALMLNNLSVIKRPVVETGD
                     GAVLVGFDVERYAGLR"
     gene            complement(818..1339)
                     /locus_tag="VFV25_12455"
     CDS             complement(818..1339)
                     /locus_tag="VFV25_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1394177.1"
                     /translation="MNPADFRASYAAAALAAGPVVALVAGCTGYSPAGFTAGATREQV
                     AQAMGQPTGAYPLPGGGSRVEYARGPFGKQTYMLDFDAQGRLQQWQQVLTEKNFDALP
                     AGIDQNEVLMRLGRPSSRFGIHYQNETVWAYRYESPFCKWFQVGIGPDGKVLDTAYGT
                     DPLCDHDGREPRR"
     gene            1540..>3018
                     /locus_tag="VFV25_12460"
     CDS             1540..>3018
                     /locus_tag="VFV25_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003262850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY1394178.1"
                     /translation="MKIVCIGGGPAGLYFALLMKQADAAHEITVVERNRPYDTFGWGV
                     VFSDQTLGNLRSADPVTAAQILDAFNHWDDIEVNIRGHKIRSGGHGFCGIGRKRLLNI
                     LQKRCEELGVKLVFETDVSSEEAYADADLVIASDGLNSRIRSKYAAVYQPDIDTRRCR
                     FVWLGTHKLFEAFTFAFEETEHGWFQAHAYRFDETTSTFIVEAPEEVWLKAGLDEMEK
                     EEAIAFCERLFAKYLDGQKLMSNATHLRGSAQWIKFPRVVCQRWVHHNGRAPVVLMGD
                     AAHTAHFSIGSGTKLALEDAIELARAINARPGELMQALQDYEAVRSVEVLRIQNAARN
                     STEWFENVARYVNLPPEQFAYSLLTRSQRISHENLRLRDKGYVEAYEDWIAQRAGVQR
                     TSTQSSVPPMFTPFNVRSVTLKNRVIVSPMAQYSCVDGQPADYHLAHLGARAMGGAGL
                     VVTEMTCPSPDARITPGCPGLWSEAQRDGWKRIVEFVHANSDA"
BASE COUNT          505 a         1004 c          998 g          511 t
ORIGIN      
        1 ggcgtgcaga agctcaagga cgcgacccca gccggcgtgc tcctcggtag cggcaagctc
       61 gcgaccgagc tggaccggct ggatctgatc gacgagtaca agttcctcgt ccaccccagg
      121 atcgccggcc acggcccaac cctgtacgag agcgggctgc ccagcacgcg acggctcgag
      181 ttgatctcgg cgaagccgct ccgcagcggc gcggtcgcca tgcactaccg gcgcgcgcgc
      241 tgactcggag gtgggtcctg tcgcctaaca atgcgctgca ctcggacagg cctcgcgttg
      301 ctcggcccgc cggtgagcgc gggcgttaaa tgaccccttt gaggctccga tttcagctac
      361 ggctttgacc gcttcgggtc gcgtgtcgcc ggtcgagcgt gaaaggcgaa tgaccggagt
      421 cagcctgaag caggctcacg acgtggtcag tgtggaacag cggctcagcg caagcccgcg
      481 tagcgctcca catcaaagcc caccagcacc gcgccgtcgc ccgtctccac caccggccgc
      541 ttgatgacgc tcaggttgtt gagcatcaag gccttggccg aggccgcatc gacgacggcg
      601 gcctgctgct cgggatccaa cttgcgccag gtcgtgccct ggcgattgag caggcgctcc
      661 cagcccaggg tggcgatcca ggcgtcgagc cgctcggcgg ccagccccag cttcttgaag
      721 tcatggaagt cgtgagccac gccctcggcc tgcagccagg cgcgcgcctt cttcacggtg
      781 tcgcagttgg aaattccgta gagcttgatg ttgctggtca tcttcggggt tcgcgtccgt
      841 cgtggtcgca cagtgggtcg gtaccgtagg cggtgtcgag caccttgccg tcggggccga
      901 tgccgacctg gaaccactta cagaaggggc tctcgtagcg gtaggcccag acggtctcgt
      961 tctggtagtg gatgccgaag cgcgacgagg gccgccccag ccgcatcagg acctcgttct
     1021 ggtcgatgcc ggcgggcagc gcatcgaagt tcttctcggt cagcacctgc tgccattgtt
     1081 gcaaacgccc ctgggcgtcg aagtccagca tgtaggtctg cttgccgaag gggccgcgcg
     1141 cgtattcgac gcggcttcct ccgccgggca agggataggc gcccgtgggt tggcccatgg
     1201 cctgggccac ctgctcgcgc gtggcgccgg ccgtgaagcc agccggcgaa tagccggtgc
     1261 agccggcgac cagagccacg actgggccgg cggccagggc ggcggctgcg tagctcgctc
     1321 tgaaatcggc agggttcatg gacgtctcct gaagacgcgc gacgccattg tcgttcgcgt
     1381 gcgggcttgg accccggcgc cgcgcaaaga gtccgtgtat gccgcgccgg gccgcagcgg
     1441 ttgcgtttcg gcttgcaaag tccggcctcc ttgcctacac tagtttagac ttaaattatt
     1501 tagcttccat cttgtctgga tggaagggag accgcgtcca tgaagatcgt gtgcataggc
     1561 ggcggcccgg cgggcctgta tttcgcgctg ctgatgaagc aggccgacgc ggcgcacgag
     1621 atcacggtcg tcgaacgcaa tcgcccctat gacaccttcg gctggggcgt ggtgttctcc
     1681 gatcagacgc tgggcaatct gcgctcggcc gacccggtga cggcggcgca gattctcgat
     1741 gccttcaacc attgggacga catcgaggtc aacatccgcg gccacaagat ccgctcgggc
     1801 ggccatggct tctgcggcat cgggcgcaag cgcctgctca acatcctgca gaagcgctgc
     1861 gaggagctgg gcgtcaagct ggtcttcgag accgatgtga gcagcgaaga ggcctatgcc
     1921 gacgccgacc tggtcatcgc cagcgacggg ctgaacagcc gcatccgcag caagtacgcc
     1981 gccgtctacc agcccgacat cgacacgcgg cgctgccgct tcgtctggct gggcacccac
     2041 aagctcttcg aggccttcac cttcgccttc gaagagaccg agcatggctg gttccaggcc
     2101 cacgcctacc gcttcgacga gacgacctcg accttcatcg tcgaagcgcc cgaggaagtc
     2161 tggctcaagg ccggcctaga cgagatggag aaggaggagg ccatcgcctt ctgcgagcgg
     2221 ctgttcgcca agtacctgga cggccagaag ctgatgtcca acgccacgca tctgcgcggc
     2281 tcggcgcaat ggatcaagtt cccgcgcgtg gtctgccagc gctgggtgca ccacaacggt
     2341 cgcgccccgg tggtgctgat gggcgacgcc gcgcacactg cgcatttctc gatcggctcg
     2401 ggcaccaagc tcgcgctgga agatgcgatc gagctggcgc gcgccatcaa tgcccgcccc
     2461 ggcgagctga tgcaggcgct gcaggactac gaggcggtgc gcagcgtcga ggtgctgcgc
     2521 atccagaatg ccgcgcgcaa ctccaccgaa tggttcgaga acgtggcgcg ctatgtgaac
     2581 ctgccgcccg agcagttcgc ctattcgctg ctcacgcgca gccagcgcat cagccacgaa
     2641 aacctgcggc tgcgcgacaa gggctacgtc gaggcctacg aggattggat tgcgcagcgc
     2701 gcgggcgtgc agcgcacttc gacgcagtcg tcggttccgc cgatgttcac gccgttcaac
     2761 gtacgcagtg tcacgctcaa gaaccgcgtc atcgtctcgc cgatggcgca gtactcctgc
     2821 gtcgatggac agccggccga ctatcacctc gcacacctgg gcgcgcgcgc catgggtggc
     2881 gccggcctcg tcgtgacgga aatgacctgc ccgtcgcccg atgcgcgcat cacgcccggc
     2941 tgccccgggc tgtggagcga agcgcagcgc gacggctgga agcgcatcgt cgagttcgtc
     3001 catgccaaca gcgacgcg
//
LOCUS       DASUCY010000628         3451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1186996, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000628 DASUCY010000000
VERSION     DASUCY010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186996"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(230..1183)
                     /locus_tag="VFV25_12465"
     CDS             complement(230..1183)
                     /locus_tag="VFV25_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY1394179.1"
                     /translation="MTGTTSSSTVASPAPFLAPAALPVLALLFNAFVWGISWWPFRQL
                     QARGLHPLWATALIYAMTAVFICLWRPAALRQLLGTPVLWVLVLASGMTNAAFNWAVS
                     IGDVVRVVLLFYLMPLWALLLARLLLHEPLTKLAGLRIALALTGAMLVLEPAGGAGWP
                     LPSSLPDWLGVAGGMSFALNNVMLRREARRPEAGRALAMFMGGVLVAGSMAALLASAG
                     RLPWPPVPAPGWMLPALALGAAFLVSNLALQYGAARLRASVTAVVMLSEILFASASAA
                     LLGSGVLSSQSLLGGGLIMGAAALSVMERHAGSGSQSQIGT"
     gene            1312..2181
                     /locus_tag="VFV25_12470"
     CDS             1312..2181
                     /locus_tag="VFV25_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1394180.1"
                     /translation="MQPLIVFQPLQVLNDRVDMAPYARKFLAQGDSWFSFGSLNPAKN
                     SNLLFEMAFAQSFCAIDCASPGDTLRHMVSMSQEPQFAGLLQGRVAWPWDGLILSAGG
                     NDLIAALDVAPDVADPALRLLRVEAEWGPESDGAARYVSDAGWQTFCTYLLANLDVLI
                     RMREAGPSAGKPIFMHTYAYPVPRPAGPGLGLHPWLMPALQAYGVPDADWTAVAHELV
                     SRFVQLLQSAAANAARFPNLNVFDTTAIAITPADPASTGESGDWLNEIHLTWQGYEKL
                     ALPWAQAIEAAVA"
     gene            complement(2213..>3451)
                     /gene="lplT"
                     /locus_tag="VFV25_12475"
     CDS             complement(2213..>3451)
                     /gene="lplT"
                     /locus_tag="VFV25_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HEY1394181.1"
                     /translation="SSLADNALLVAAIDLLKTQGSPAWQVPALTPMFALFYVVLAPFV
                     GAFADAVPKGKVMFASNTIKVVGCFMMLFGTHPLLAYAVVGLGAAAYSPAKYGILTEL
                     LPPSQLVKANGWIEGLTIASIILGVVLGGQLIGPRLAPMLLGIDLPWMGDYIDTPPEA
                     AIAAISTLYVIAAIFNLFIPRTDAPLQPVPRSPLALVRDFSTCNARLWSDKLGQISLA
                     TTTLFWGISGNLRIIVFAWAAAALSYTTTQASNLQGVVAIGMAVGVVFASLRMRLDRA
                     PSVIPLGIAMGLMLIVMNAIHNVYLAAPFLVVLGAIGGFLVVPMNALLQHRGHNLMGA
                     GRSIAVQNFNEQAAILGLGALYTALTRVGLSAFGAITIFGLMVAATMWLIGRWHKHNS
                     RVDREEVEHLLMLARSDVQH"
BASE COUNT          604 a         1183 c         1177 g          487 t
ORIGIN      
        1 ggtcggtgct cggtgtcctg tggggcggcg tgcggccggc ttcgaagacc ggcgccatcc
       61 atgctgctgc ggcgagcggg ggcatgggag cgaaccatac accagcgagg ccggggcttg
      121 tgacgaatcg ctccgggcgg attgctatgt cctgaagtga tcctctgtac caagagagct
      181 cgacaaagcc gacaagaatt ggccactgac agtggagcgc gtggaggagc tatgtcccga
      241 tttgcgactg gctgcccgag cccgcgtgcc gctccatcac cgacaaggcc gccgcgccca
      301 tgatcagtcc gccgccgagc agcgactgag agctcaagac cccgctgccc agcaaagcgg
      361 ccgaggcgct ggcgaacagg atttcgctca gcatcaccac tgccgtgacg ctggcgcgca
      421 ggcgcgccgc gccgtattgc agcgccagat tggagaccag gaaggccgcg cccagggcca
      481 gggccggcag catccagccc ggcgccggga cgggcggcca gggcaggcgg ccggcgctgg
      541 ccaacagcgc cgccatgctg ccggccacca gcacgccgcc catgaacatc gccagcgcgc
      601 ggcccgcctc gggccggcgc gcctcgcgcc tgagcatcac gttgttgagc gcgaagctca
      661 tgccgccggc cacacccagc cagtccggca ggctgctcgg cagcggccag ccggcgccgc
      721 cggccggctc gagcaccagc attgcgcccg tcagcgccag cgcgatgcgc agccccgcca
      781 gcttggtcag cggttcatgc agcagcagcc gtgccagcag cagggcccat agcggcatca
      841 ggtagaaaag cagcaccacg cgcaccacgt cgccgatgct gaccgcccag ttgaaggccg
      901 cattggtcat gcccgaggcg agcaccagca cccacagcac cggcgtgccc agcagctgcc
      961 gcagcgccgc cggccgccag aggcagatga agaccgccgt catcgcatag atcagcgccg
     1021 tggcccacag cgggtgcagg ccccgcgcct gcaattgccg aaagggccac caggagatgc
     1081 cccacacgaa ggcgttgaac agcagcgcca gcacgggcag ggcggcgggg gcgaggaaag
     1141 gtgcgggcga ggcgacggtg gaggaagacg tggtgccggt catgagcctc gcattgtccc
     1201 agggcccgtt gagcggggct cagccctccc gcacccgcag ggtggcgagg caggggcgcc
     1261 gcgcctaagt ttgtcgctgt cgcccggccg tcgccgggcg gggaggacag catgcaaccc
     1321 ctgattgttt tccagcccct ccaggtcttg aacgaccggg tggacatggc gccttatgcg
     1381 cgcaaattcc tggcccaggg cgattcctgg ttctccttcg gctcgctcaa cccggccaag
     1441 aactccaatc tgctcttcga aatggccttc gcgcagagct tctgtgccat cgactgcgcc
     1501 tcgcccggcg acacgctgcg ccacatggtg tcgatgtcgc aggagccgca gttcgccggc
     1561 ttgctgcagg ggcgcgtcgc ctggccatgg gacgggctga tcctgtcggc cggcggcaac
     1621 gatctgatcg cggcgctcga cgtggccccc gacgtcgccg acccggcgct gcgcctgctg
     1681 cgcgtcgagg ccgaatgggg gccggagagc gatggcgcgg cacgctatgt cagcgacgcc
     1741 gggtggcaga ccttctgcac ctatctgctc gccaacctcg atgtgctgat ccggatgcgc
     1801 gaagccggac cctcggcggg caagccgatc ttcatgcaca cctatgccta tccggtgccg
     1861 cgcccggccg gacccggtct cggcctgcac ccctggttga tgcctgcgct tcaggcctac
     1921 ggcgttcccg atgccgactg gaccgccgtc gcgcacgaac tggtctcgcg ctttgtccag
     1981 ctgctgcaga gtgcggcggc caatgcggcg cgctttccga acctcaatgt cttcgacacg
     2041 acggccattg ccatcacgcc cgccgatccg gcctcgaccg gcgaaagcgg cgactggctc
     2101 aacgagatcc acctgacttg gcaaggctac gagaagcttg cgctgccctg ggcgcaggcg
     2161 atcgaggcgg cagtggcttg aatgaaggga agaagggtga gcgcggtgcc gctcaatgct
     2221 gcacgtcgct gcgcgccagc atcagcagat gctcgacctc ttcgcggtcg actctgctgt
     2281 tgtgcttgtg ccagcggccg atcagccaca tcgtggcggc caccatcagg ccgaagatcg
     2341 tgatcgcgcc gaaagccgac aggcccacgc gggtcagcgc cgtatagagc gcgcccaggc
     2401 ccaggatggc ggcctgctcg ttgaagttct gtaccgcgat cgagcggccg gcgcccatca
     2461 ggttgtggcc gcggtgctgc agcagcgcgt tcatcggcac cacaaggaag ccgccgatcg
     2521 cgcccaacac cacgaggaag ggcgcggcca gatagacgtt gtggatcgcg ttcatgacga
     2581 tcagcatcaa gcccatcgcg atgccgagcg gaatcacact cggcgcgcgg tccagccgca
     2641 tgcgcaacga cgcgaacacc acgcccaccg ccatgccgat cgccaccacg ccttgcaggt
     2701 tggaggcctg cgtggtggtg taggacagcg ccgctgccgc ccaggcgaag acgatgatgc
     2761 gcaggttgcc cgagatgccc cagaacagcg tcgtcgtggc gagcgagatc tggcccagct
     2821 tgtcgctcca cagccgcgca ttgcaggtcg agaagtcgcg caccagggcc agcgggctgc
     2881 gcggcaccgg ctgcagcggc gcgtcggtgc gcgggatgaa gagattgaag atggccgcga
     2941 tgacgtagag cgtgctgatc gccgcgatcg ccgcctcggg cggcgtgtcg atgtagtcgc
     3001 ccatccacgg caggtcgatg cctagcagca tgggggcgag ccgggggccg atgagctggc
     3061 cgccgagcac cacgccgagg atgatagagg cgatcgtcag cccctcgatc cagccgttgg
     3121 ccttgaccag ctgcgaaggc ggcagcagct cggtgaggat gccgtacttg gccggcgaat
     3181 aggccgccgc acccaggccc acgacggcat aggccagcaa cggatgggtg ccgaacagca
     3241 tcatgaagca gcccaccacc ttgatggtgt tggacgcgaa catgaccttg cccttgggaa
     3301 cggcgtcagc gaaggcgccg acgaagggcg ctagcaccac ataaaacagc gcgaacatcg
     3361 gcgtcagcgc cggcacctgc cacgccggtg agccctgggt cttgagcagg tcgatcgcag
     3421 ccaccagcaa ggcgttgtct gccagcgagc t
//
LOCUS       DASUCY010000629         3975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_14653, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000629 DASUCY010000000
VERSION     DASUCY010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14653"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /locus_tag="VFV25_12480"
     CDS             complement(<1..994)
                     /locus_tag="VFV25_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEY1394182.1"
                     /translation="MALVEVKVPQLSESVAEATLLQWKKKPGEAVAMDEILIEIETDK
                     VVLEVPAPQAGVLSEIVKKDGETVVADELIARIDTEAKAGAAAPAAAQPQVQAAAPAA
                     APAVAAAAVAAAASGSKSSVAMPAAAKLLADHQLSVGQVAGTGKDGRVTKGDVLGALE
                     AGATKVIVQSPVSTPVTAKKPALQQVMVPIAAELGERPEQRVPMSRLRARIAERLLQS
                     QATNAILTTFNEVNMAPVMEMRKKFQEKFEKEHGVKLGFMSFFVKAAVAALKKYPVIN
                     ASVDGNDIVYHGYFDIGIAVGSPRGLVVPVLRNADQMSFADIEKKISEYGKKAAD"
     gene            complement(1094..3952)
                     /locus_tag="VFV25_12485"
     CDS             complement(1094..3952)
                     /locus_tag="VFV25_12485"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEY1394183.1"
                     /translation="MMQQYRSNSYLFGGNAPYVEELYEAYLDNPGSVPDKWRAYFDAL
                     QHVPAVDGSEARDVAHAPVVESFAQRAKANAFALRASASELAVARKQVHVQSLIAAYR
                     SLGARWADLDPLKRAERPRIPELEPAFYDLSESDMDITFSATNTYFTTAEQQTLREIL
                     QALRETYCGSIGAEFMHITEPVEKRWWQEKLESVRAKPSFSPVQKIHILGRLTAAEGL
                     ERYLHTKYVGQKRFSLEGGESFIASMDELVQRGGERGVQEFVIGMAHRGRLNVLVNTL
                     GKAPKDLFSEFDHTAREDLPAGDVKYHQGFSSDVTTPGGPVHLSLAFNPSHLEIVNPV
                     VEGSVKARLDRRGDKEGDQVLPVLVHGDAAFAGQGVIMETLALAQTRGYYTGGTLHIV
                     INNQIGFTTSDPRDTRSTLYCTDVVKMIEAPVLHVNGDDPEAVVLCTQLALDYRQQFK
                     KDVVVDIICFRKLGHNEQDTPALTQPLMYKKIAAHPGTRKLYGEKLVAQGLLPAEGLD
                     EMVKAFRAAMDAGKHTVDPVLTNYKSKYAVDWAPFLGKKWTDAADTALPLAEIKRLAE
                     RITTVPPSFKAHPLVEKVLADRAAMGRGEVNVDWGMGEHLAFASLVASGYPVRLSGED
                     SGRGTFTHRHAVLHDQNREKWDIGTYIPLQNVAENQAPFVVIDSILSEEAVLGFEYGY
                     ASADPHTLTIWEAQFGDFVNGAQVVIDQFIASGEVKWGRANGLTLMLPHGYEGQGPEH
                     SSARLERFMQLAADNNIQVVQPTTASQIFHVLRRQQVRAFRKPLVIMTPKSLLRNKDA
                     TSPLSEFTRGEFRTVIPELNAEIDAAKVKRVIACSGKVYYDLVKKREEKKAPDVAIIR
                     VEQLYPFPHKAFGAELKKYPNLSDIVWCQDEPQNQGAWFFVQHYIHENMLEGQKLGYA
                     GRPASASPAVGYAHLHQEQQKALLDQAYAKLKGFVLTK"
BASE COUNT          592 a         1288 c         1329 g          766 t
ORIGIN      
        1 cgtcggccgc cttcttgccg tactcggaga tcttcttctc gatgtcggcg aagctcatct
       61 ggtcggcatt gcgcagcacc ggcaccacca ggccgcgcgg cgaacccacc gcgatgccga
      121 tgtcgaagta gccgtggtag acgatgtcgt tgccgtccac gctggcattg atcaccgggt
      181 acttcttgag cgcggcgacc gcagccttga cgaagaagct catgaagccg agcttgacgc
      241 catgctcctt ctcgaacttc tcctggaact tcttacgcat ctccatcacc ggtgccatgt
      301 tcacctcgtt gaaggtggtc aggatggcgt tggtggcctg ggactgcagc aggcgctcgg
      361 cgatgcgggc gcgcagccgg ctcatcggca cacgctgttc ggggcgctcg cccagctcag
      421 ccgcgatcgg caccatcacc tgctgcaatg caggtttttt cgcagttacc ggtgtactta
      481 caggcgattg cactatcact ttggtagcgc ccgcctcgag cgcgccgagc acgtcgccct
      541 tggtgacgcg gccgtccttg ccggtgccgg cgacttggcc cacgctcagc tggtggtcgg
      601 ccagcagctt ggcggcagca ggcatcgcga ccgaggactt cgagcctgag gcagctgctg
      661 cgacggccgc tgcggcaacg gccggtgcag cggcgggggc agcagcctgc acttgcggct
      721 gcgcggcagc aggcgcggcg gcgccggcct tggcttcggt gtcgatgcgc gcgatcagct
      781 cgtcggcgac cacggtctcg ccgtctttct tgacgatctc ggacagcacg ccggcctggg
      841 gtgccggcac ttcgagcacg accttgtcgg tctcgatctc gatgaggatt tcatccatcg
      901 ccacggcttc gccgggcttc ttcttccact gcagcagggt ggcctcggcc accgattcgg
      961 acaactgggg aaccttgact tcaacaagcg ccatgatgcg tgtcttcctg ggtgattctt
     1021 cgcgggtgcg tgcgtgattc ttgtgcgcac aggcgcgcgc actcatcgct gagcacgcgt
     1081 cgcctggctt gccttatttc gtgagaacga agcccttgag cttggcgtag gcctggtcga
     1141 gcaaggcctt ctgctgctcc tggtgcagat gggcatagcc caccgccggc gaggccgagg
     1201 ccggacggcc ggcatagccg agcttctggc cctcgagcat gttctcgtgg atgtagtgct
     1261 gcacgaagaa ccaggcgccc tggttttgcg gctcgtcctg acaccacacg atgtcgctca
     1321 agttcgggta cttcttgagc tcggcgccga aggccttgtg cgggaaggga tagagctgct
     1381 cgacgcggat gatggccacg tcgggtgcct tcttctcctc gcgcttcttg acgaggtcgt
     1441 aatagacctt gcccgagcag gcgatcacgc gcttgacctt ggccgcgtcg atctcggcgt
     1501 tcagttccgg gatcacggtg cggaactcgc cgcgggtgaa ctccgacagc ggcgaggtcg
     1561 cgtccttgtt gcgcagcagc gacttgggcg tcatgatcac cagcggcttg cggaaggcgc
     1621 gcacctgctg gcggcgcagc acgtggaaga tctggctggc cgtcgtcggc tgcacgacct
     1681 ggatattgtt gtcggccgcc agctgcatga agcgctccag gcgagcggac gaatgctcag
     1741 gcccctggcc ctcgtagccg tgcgggagca tcagcgtcag gccgttggcg cggccccact
     1801 tcacttcgcc ggaagcgatg aactggtcga tgaccacctg ggcgccgttg acgaagtcgc
     1861 cgaactgggc ttcccagatc gtcagcgtgt gcggatcggc gctggcatag ccgtattcga
     1921 agccgagcac cgcctcctcg gacaggatgg agtcgatgac gacgaagggt gcctggttct
     1981 ccgccacgtt ctgcagcggg atataggtgc cgatgtccca cttctcgcgg ttctggtcgt
     2041 gcagcacggc gtggcggtgc gtgaacgtgc cccgccccga gtcctcgccc gacagccgca
     2101 ccggatagcc gctggccacc agcgaggcga aggccaggtg ctcgcccatg ccccagtcga
     2161 cgttgacttc gccgcggccc atcgcggcgc gatcggccag caccttctcg accagcgggt
     2221 gcgccttgaa gctgggcggc accgtggtga tgcgctcggc caggcgcttg atctcggcca
     2281 gcggcagcgc ggtgtcggcc gcatcggtcc acttcttgcc gaggaagggc gcccagtcga
     2341 ccgcgtactt gctcttgtag ttggtcagca ccggatcgac cgtgtgcttg ccggcgtcca
     2401 tcgcggcacg gaaggccttt accatctcgt cgagaccctc ggccggcagc aggccctggg
     2461 ccaccagctt ctcgccgtag agcttgcgcg tgccgggatg ggcggcaatc ttcttgtaca
     2521 tcagcggctg ggtcagcgcc ggcgtgtcct gctcgttgtg gcccagcttg cggaagcaga
     2581 tgatgtcgac caccacgtcc ttcttgaact gctggcggta gtccagcgcg agctgggtgc
     2641 acagcacgac cgcctcgggg tcgtcgccgt tcacgtgcag caccggcgcc tcgatcatct
     2701 tgacgacgtc ggtgcagtac agcgtcgagc gcgtgtcgcg cggatcgctg gtggtgaaac
     2761 cgatctggtt gttgatgacg atgtgcagcg tgccgccggt gtagtagccg cgcgtctgcg
     2821 ccagcgccag cgtctccatg atgacgccct ggccggcgaa ggccgcgtcg ccgtgcacca
     2881 gcacgggcag cacctggtcg ccctccttgt cgccgcggcg gtcgaggcgg gccttgaccg
     2941 agccctccac caccgggttg acgatttcca gatgcgaggg gttgaaagcc aggctcaagt
     3001 gcaccgggcc gccaggcgtc gtgacgtcgc tggaaaagcc ttggtggtat ttcacgtcgc
     3061 cggcgggcag gtcctcgcgg gcggtgtgat cgaactcgga gaacaggtcc ttgggcgcct
     3121 tgcccagggt gttgaccagc acattgaggc ggccgcggtg ggccatgccg atcacgaact
     3181 cctgcacgcc ccgctcgccg ccgcgctgca ccagctcgtc catcgaggcg atgaagctct
     3241 cgccgccttc cagcgagaag cgcttctggc cgacgtactt ggtgtgcaga tagcgctcca
     3301 ggccctcggc ggcggtcagg cgccccagga tgtggatctt ctgcacgggc gagaagctcg
     3361 gcttggcgcg cacgctttcc agcttctctt gccaccagcg cttttcaacc ggctcggtga
     3421 tgtgcatgaa ctccgcgcct atgctgccgc aataggtctc gcgcagggct tgcaggatct
     3481 cgcgcagcgt ctgctgctcg gcggtcgtga aatacgtgtt cgtcgcgctg aacgtgatgt
     3541 ccatgtcgga ctcgctcagg tcgtagaacg ccggctccag ctcgggaatc cgggggcgct
     3601 cggcgcgctt gagcggatcg aggtcggccc agcgcgcacc cagcgagcgg taggccgcga
     3661 tcagcgactg gacatggacc tgcttgcggg cgaccgcgag ttcgctggcc gaggcgcgca
     3721 gagcgaaggc attggccttg gcacgctggg cgaaggattc gacgaccggt gcatgggcca
     3781 cgtcgcgggc ttcggagccg tccaccgccg gcacatgctg gagcgcgtcg aaataggcgc
     3841 gccatttgtc gggcaccgag cccgggttgt cgaggtaggc ctcgtacaac tcttccacat
     3901 agggcgcatt gcccccgaac aggtacgagt tcgacctgta ttgctgcatc atctttcgct
     3961 caccttttgc gctgg
//
LOCUS       DASUCY010000630         3642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_576563, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000630 DASUCY010000000
VERSION     DASUCY010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576563"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1221)
                     /locus_tag="VFV25_12490"
     CDS             complement(<1..1221)
                     /locus_tag="VFV25_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1394184.1"
                     /translation="MASLALTACNLVSPTSGAIRQIARAQRLASDTSDPKALPPADAE
                     HGWQIVQLPDGWPGEQPAGTTAYWYRVSFQARAPLADEALHVRVLTPAALYAVNGRVV
                     SITPEMQSRSGAAKPVQVPLPRDALREGDNELLVRLSVLPDRAAYLGSLRIGPAAEVE
                     AEYERFIFFKRTLRQAVVVAALLLAVVLASIWSARRGELLYLLFALVCLGWAHQTHNY
                     YASAPPWPEPYWGISIALGDYLTESLMCLFVLRYTDLLRRWHVLALAAYVLLSFGITI
                     RGGLSDTHWWWDWYSQPWEYTPFAISALYMALLVWHAWRTRRGADALMALACVLMIAA
                     SWRDTAPFSTAPVSEELALTPYAYGIVIAVFSARLVRRFIDSVAEAEALNHQLEGRIA
                     ERTQEIRRSLEALAV"
     gene            complement(1266..2300)
                     /locus_tag="VFV25_12495"
     CDS             complement(1266..2300)
                     /locus_tag="VFV25_12495"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEY1394185.1"
                     /translation="MPLVPYPLARPFLFGLDPEQAHELTLGSIAALQNTPLQCLWAQA
                     RVEAPLTIAGLKFPNRIGLAAGLDKNGRCIDGLGAMGFGFIEVGTVTPKAQPGNPRPR
                     MFRLPQADALINRLGFNNEGLAAFIANVERSRSFRQRGGILGLNIGKNAATPIENAAD
                     DYLTALSGVYPHADYVTVNISSPNTKNLRALQSDEALDALLGALQQRRQQLVIEHQRA
                     VPMFVKIAPDLDEAQIEVIAQTLSKNAIDGVIATTTTITREAVRGLMHAEEAGGLSGR
                     PVFEASNRVIRQLRAHLGKGFPIIGVGGVLSGEDARAKLEAGANLVQVYTGLIYRGPA
                     LVRECALALR"
     gene            2372..3031
                     /gene="rpiA"
                     /locus_tag="VFV25_12500"
     CDS             2372..3031
                     /gene="rpiA"
                     /locus_tag="VFV25_12500"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEY1394186.1"
                     /translation="MTQDELKALVGRAAVDYVLPGSIVGVGTGSTVNCFIDELAKIKD
                     RIKGAVSSSLKSTERLRAHGIQVFDANEVETLPVYIDGADEIDHGGNMIKGGGAALTR
                     EKIVADMAERFVCIADESKLVKLLGKFPLPVEVIPMAQALVSRRLRALGGEPRLRAGV
                     TTDNGNLILDVTGLAIADPAAVEAQINQWPGVVTVGIFARNRASVCLLGTSSGVRTLT
                     F"
     gene            complement(3109..>3642)
                     /locus_tag="VFV25_12505"
     CDS             complement(3109..>3642)
                     /locus_tag="VFV25_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEY1394187.1"
                     /translation="HALYKIVGPDGKVTYTDRPPVPPGGKVQSLTPSGAPVSDEALPY
                     ELRQVASRYPVTLYTAEKCAPCDSGRNYLRQRGIPFAERTVSTNADIDAMQRVEGGAE
                     IPTLRIGGQQLKGFSDAEWGSYLDAAGYPKQSALPAGYVFPPAQPMVSKDVIPAPAAA
                     PRPAPAPAPAASGGIRF"
BASE COUNT          597 a         1170 c         1312 g          563 t
ORIGIN      
        1 cacggccagg gcctcgagcg agcggcggat ctcctgcgtg cgctcggcga tgcggccttc
       61 gagctgatgg ttgagcgcct cggcctcggc cacgctgtcg atgaagcggc gcaccaggcg
      121 ggccgagaac actgcgatca cgatgccgta ggcataaggc gtcagcgcga gctcctcgct
      181 aacgggcgcg gtgctgaagg gggccgtatc ccgccacgag gcggcgatca tcagcacgca
      241 ggcgagcgcc atcagcgcat ccgcgccgcg gcgcgtgcgc caggcgtgcc acaccagcaa
      301 cgccatgtag agcgccgaga tcgcgaaggg cgtgtattcc caaggctggc tgtaccaatc
      361 ccaccaccaa tgcgtatcgc tgagcccgcc ccttatcgtg atgccgaagg acagcagcac
      421 ataggcggcc agcgccagca cgtgccagcg cctgagcagg tcggtatagc gcagcacgaa
      481 gaggcacatc agcgactcgg tgagatagtc gcccagcgcg atgctgatgc cccaataggg
      541 ctccggccag ggcggcgcgc tggcgtagta gttgtgcgtc tgatgggccc agcccaggca
      601 gaccagggcg aacagcagat acagcagctc gccgcgccgc gccgaccaga tcgaggcgag
      661 caccacggcc agcagcagcg ccgccacgac cactgcttgc cgcagcgtcc gcttgaagaa
      721 gatgaagcgc tcgtactcgg cttcgacctc ggcggccggg ccgatgcgca ggctgcccag
      781 ataggcggca cgatcgggca gcacgctcag gcgcaccagc agctcgttgt cgccttcgcg
      841 cagcgcatcg cgcggaagcg gcacctgcac cggtttggcc gcacccgaac ggctttgcat
      901 ctcgggcgtg atcgagacga cgcggccgtt gacggcatag agcgcggctg gcgtcagcac
      961 gcgaacgtgc agggcctcgt cggccagcgg cgcacgtgcc tggaagctca cgcggtacca
     1021 gtaggccgtg gtgccggccg gctgctcgcc gggccagccg tcgggcagct gcacgatctg
     1081 ccagccatgt tcggcgtctg ccggcggcag ggccttgggg tcgctcgtgt ccgaggccag
     1141 gcgctgggcc cgtgcgattt gccggatggc gccgctagtc ggcgagacga ggttgcaggc
     1201 cgtgagcgcg aggctggcca agagccaggc agccaggccg gccgcccgcc gcgccaccga
     1261 caaccttagc gcagcgcaag cgcgcattcg cgcaccaggg ccgggccgcg gtagatcagc
     1321 ccggtgtaga cctgcaccag gttggcgccg gcctcgagct tggcgcgcgc gtcttcaccg
     1381 ctcaacacgc cgcccacgcc gatgatgggg aagcccttgc ccaagtgcgc gcgcagttgg
     1441 cggatcacgc ggttgctggc ctcgaacacc gggcgcccgc tgaggccgcc ggcctcctcg
     1501 gcatgcatca ggccgcggac ggcctcgcgc gtgatcgtcg tggtggtggc gatcacgccg
     1561 tcgatcgcgt tcttgcttag cgtctgtgcg atcacctcga tctgcgcttc gtccaggtcg
     1621 ggtgcgatct tgacgaacat gggcaccgcg cgctggtgtt cgatcacgag ctgctggcgc
     1681 cgctgttgca gcgcgccgag cagggcatcc agcgcctcgt cgctctgcag cgcacgcagg
     1741 ttcttggtgt tggggctgga gatgttgacc gtcacatagt cggcatgcgg atagacgccg
     1801 ctcaaggccg tcaggtagtc gtcggcggcg ttctcgatcg gcgtggccgc gttcttgccg
     1861 atgttgagac cgaggatgcc gccgcgctgc cggaaggagc gcgagcgctc gacgttggcg
     1921 atgaaggcgg ccagcccctc gttgttgaag cccaggcggt tgatcagcgc atcggcctgc
     1981 ggcaggcgga acatgcgcgg cctggggttg cccggctggg ccttgggcgt gaccgtgccg
     2041 acctcgatga agccgaagcc catcgcgccc agcccgtcga tgcagcggcc gttcttgtcc
     2101 agacccgcgg ccaggccgat gcggttgggg aacttcaggc ccgcaatcgt gagtggcgcc
     2161 tccacccgcg cctgggccca caggcattgc agcggcgtgt tctgcagcgc ggcgatcgag
     2221 cccagtgtca gctcatgcgc ctgctcggga tcgaggccga agaggaaggg tcgggcaagc
     2281 gggtagggga cgagaggcat ggaggctgtc ttggataatc gcgggattgt ctcaaacgca
     2341 ggcgcaggcc ccacccgaca ggatgacgac gatgacgcaa gacgaactca aggccctggt
     2401 cggccgcgcc gccgtggact atgtgctgcc gggcagcatc gtcggtgtgg gcaccggctc
     2461 cacggtgaac tgcttcatcg acgagctggc gaagatcaag gacaggatca agggcgccgt
     2521 gtccagctcg ctcaagagca ccgagcggct gcgggctcat ggcatccagg tgttcgacgc
     2581 caacgaggtc gagacgctgc cggtctacat cgacggcgcc gacgagattg atcacggcgg
     2641 caacatgatc aagggcggcg gcgccgcgct cacgcgcgag aagatcgtgg ccgacatggc
     2701 cgagcgcttc gtctgcatcg cggacgaatc caagctggtg aaactgctcg gcaagttccc
     2761 gctgccggtc gaggtgatcc cgatggccca ggccctggtc tcgcggcgcc tgcgagccct
     2821 gggtggcgag cccaggctgc gtgccggcgt gacgacggac aacggcaacc tgatcctcga
     2881 tgtgaccggc cttgccatcg ccgatcctgc ggcggtggag gcgcagatca atcagtggcc
     2941 aggcgtcgtg acggtgggga tttttgcgcg caacagggcg agcgtgtgcc tgttggggac
     3001 gagctcgggg gtgaggacgt tgaccttttg aggcgcggtc ggcgccttat tgcttgctga
     3061 ccgcgcgggt tgcttcgtaa agcaaaccag caaaggacag ctagagcctc agaacctgat
     3121 tccgccggat gctgcaggcg caggagccgg tgcaggtcgc ggcgccgcgg caggcgccgg
     3181 gatcacgtcc ttcgagacca tgggctgggc cggcggaaag acatagcctg ccggcagcgc
     3241 agactgcttg ggatagccgg ccgcgtcgag atagctaccc cattcggcat cggagaagcc
     3301 cttgagctgc tggccgccaa tgcgcagcgt cgggatctcg gcgccgccct cgacgcgctg
     3361 catcgcatcg atatctgcat tcgtcgagac ggtgcgttcg gcaaagggga tgccgcgctg
     3421 gcgcaggtaa ttgcgaccag agtcgcaagg tgcgcatttt tcggccgtgt agagcgtgac
     3481 cgggtagcgg ctcgcgacct ggcgcagctc gtaaggcagg gcctcgtcgg agaccggggc
     3541 gcctgagggt gtgagcgact gcaccttgcc gcccgggggg acgggcggtc ggtcggtata
     3601 ggtgaccttg ccgtcggggc cgacgatctt gtacagcgcg tg
//
LOCUS       DASUCY010000631         2719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_817366, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000631 DASUCY010000000
VERSION     DASUCY010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817366"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VFV25_12510"
     CDS             complement(<1..230)
                     /locus_tag="VFV25_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1394188.1"
                     /translation="MNSSQEGAPRRFVRAMGAVACMLLAGPLAGAEPGVSAQKLVLGM
                     SAPLTGPLAAYGGDLAQGLRLGFAEAHAAGGI"
     gene            complement(248..2641)
                     /locus_tag="VFV25_12515"
     CDS             complement(248..2641)
                     /locus_tag="VFV25_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY1394189.1"
                     /translation="MSPNPPLLDLDRFDRRSGSAIERLLFNHRWIVVVLCALVTMLLG
                     WQCATRLQINASFDKTIPARHPYIRNFLQHQNDLSGLGNAVRIAVENPQGSIYDAKYL
                     DTLRRLSDEVFLVPGVARTQMKSLWTPTTRWVGVTEEGLEGGPVIPDGYDGSARGLEQ
                     LRANVARSGEIGQIVALDARSSVIYVPLLARDAEGRALDYRAFAQRLEQLRVKYEAQG
                     VRLHITGFARIVGELIDGVRAVLLFFALAVVIATAMVFWFTRCVRSTALVVTASLVAV
                     VWQLGLLPTLGFSLDPYSILVPFLVFAIGMSHGAQKMNGIMQDVGRGLHKLVAARLTF
                     RRLFLAGLTALLADAVGFAVLLVIDIQAIRELAIAASLGVAVLIFTNLILLPVLLSYT
                     GVSAKAAVRSLRAEHAEDRHALWALLDRFTRRPAAVAAVAVALVMGVAGFAASTRLKI
                     GDLDPGAPELRADSRYNRDVAFMTGAYGASSDVLAVMVRTPEGQCAQYATLNKVDALE
                     WQLRQLDGVESTSTLALLNRRVLAGLNEGNPKWYEFLPNQAMLNTVTAGAPRGLYNDA
                     CSLLTVYVFLRDHKADTLSRVVGHVEAFARDNDSADAKFLLAAGSAGIEAATNIVVKQ
                     AWHQMLFLVYGAVVVLCFITFRSWRAVLVAVLPLVLTSMLAEALMVGLGMGVKVATLP
                     VIALGVGIGVDYALYILSVTLAQLREGRSLSQAYHRALLFTGKVVLLTGVTLAIGVIT
                     WAASPIKFQADMGVLLAFMFLWNMLGALVLLPALAHFLLKPVAVPAAAAAGTVLAAR"
BASE COUNT          474 a          967 c          906 g          372 t
ORIGIN      
        1 tggataccgc cagccgcatg ggcctccgcg aagcccagcc gcagcccctg cgccagatcg
       61 cctccatagg ccgcgagcgg tccggtgagt ggcgccgaca tgccgagcac gagcttctgc
      121 gcgctcacgc ccggctcggc cccggcgagc gggccggcca ggagcatgca ggccactgct
      181 cccattgcgc gaacgaagcg gcgcggcgcc ccttcttgcg atgagttcat cgtcagctcc
      241 atgtcggtca gcgggccgcg agcacggtcc cggctgcggc ggcggcaggg acggcgaccg
      301 gcttcaacag gaaatgcgcc agcgccggca gcagcaccag cgcgcccagc atgttccaga
      361 ggaacatgaa ggccagcagc actcccatgt cggcctggaa cttgatcggg ctggccgccc
      421 aggtgatcac gccgatggcc agcgtgacgc cggtcagcag caccaccttg ccggtgaaca
      481 gcagcgcacg gtggtaggcc tgcgacaggc tgcgcccctc gcgcagttgc gccagcgtga
      541 cgctgaggat gtagagcgcg tagtcgacgc cgatgcccac gcccagcgca atcaccggca
      601 gcgtggcgac cttcacgccc atgcccaggc ccaccatcag cgcctcggcc agcatggagg
      661 tcagcaccag cggcagcacg gccaccagca ccgcgcgcca ggagcggaag gtgatgaagc
      721 acagcaccac caccgcgcca tacaccagga acagcatctg atgccaggcc tgcttgacga
      781 cgatgttggt cgcggcctcg atgccggcgg agccggcggc gagcaggaac ttcgcgtcgg
      841 cgctgtcgtt gtccctcgcg aaagcctcca cgtggcccac cacgcgcgac agcgtgtcgg
      901 ccttgtggtc gcgcaggaac acgtagaccg tgagcaggct gcaggcgtcg ttgtacaggc
      961 cgcgcggggc gccggccgtc accgtgttga gcatggcctg gttgggcagg aactcgtacc
     1021 acttcgggtt gccctcgttc agcccggcca gcacgcggcg gttcagcagc gccagcgtgc
     1081 tggtgctctc cacgccgtcg agctggcgca gctgccactc cagcgcgtcc accttgttga
     1141 gcgttgcgta ctgggcgcac tggccctcag gtgtgcgcac catcaccgcc aggacgtcgc
     1201 tggaggcgcc gtaggccccg gtcatgaagg cgacgtcgcg gttgtagcgg ctgtcggcgc
     1261 gcaactccgg cgcgccgggg tcgaggtcgc cgatcttgag ccgcgtgctc gcggcaaagc
     1321 cggccacgcc catgaccagg gccaccgcca cggcggccac cgccgcgggc cggcgcgtga
     1381 accggtccag cagcgcccaa agcgcatgcc ggtcctcggc gtgctcggca cgcaagctgc
     1441 gtacggctgc cttggcgctc acgccggtgt agctgagcag caccggcagc aggatcaggt
     1501 tggtgaagat gagaacggcc acacccagac tcgcagcgat ggccagctcg cggatggcct
     1561 ggatgtcgat caccaggagc accgcgaagc ccaccgcatc ggccagcagc gcggtgagcc
     1621 cagcaaggaa caggcgccga aaggtcaggc gcgccgccac cagcttgtga aggccgcgac
     1681 ccacgtcctg catgatgccg ttcatcttct gcgcaccgtg gctcatgccg atcgcgaata
     1741 cgaggaaggg cacgaggatc gagtacgggt cgagcgagaa gccgagtgtc ggcagcaggc
     1801 ccagctgcca caccaccgcc accagcgagg cggtgacgac cagcgcggtc gagcgcacgc
     1861 agcgcgtgaa ccagaacacc atggccgtcg cgattacgac cgccagggcg aagaacagca
     1921 acaccgcccg cacgccgtcg atcagctcgc ccacgatcct ggcgaagccg gtgatgtgca
     1981 ggcgcacgcc ctgcgcttcg tacttcacgc gcagttgctc cagccgctgc gcgaaggccc
     2041 ggtagtccag cgcccggccc tcggcgtcac gcgccagcag cggcacgtag atcacgctcg
     2101 atctcgcatc cagcgcgaca atctggccga tctcgcccga gcgcgcgacg ttcgcgcgca
     2161 gttgctccag gccgcgcgcc gagccgtcgt agccgtccgg gatcaccggg ccgccttcca
     2221 gcccttcctc cgtaacgccg acccaacgcg tggtcggcgt ccacaacgac ttcatctgcg
     2281 tgcgcgccac tcccggcacg aggaacacct cgtcgctcag gcggcgcagc gtgtcgaggt
     2341 acttcgcgtc gtagatcgag ccctgcgggt tctcgaccgc gatgcgcacc gcgttgccga
     2401 ggccgctaag gtcgttctgg tgctgcagga aattgcggat gtacggatgc cgcgcaggga
     2461 tcgtcttgtc gaaactggcg ttgatctgaa gccgggtcgc gcactgccaa cccagcagca
     2521 tggtgaccag cgcgcacagc actacgacga tccagcggtg gttgaacaac agccgctcga
     2581 tcgccgaccc ggagcgccgg tcgaaacgat cgaggtcgag caagggaggg ttcgggctca
     2641 tcgcgcagct cctttggctg gcatcgcaat ggcaccctgc acgctgagcg caagcagccc
     2701 ttgcgcaccc agaggcagc
//
LOCUS       DASUCY010000632         2158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_191370, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000632 DASUCY010000000
VERSION     DASUCY010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191370"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VFV25_12520"
     CDS             <1..807
                     /locus_tag="VFV25_12520"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEY1394190.1"
                     /translation="SPDVCKGAIDLGFSSVMMDGSLQADGKTIASYDYNVDVTRKVVD
                     MAHKLGVTVEGELGCLGSLETMRGDKEDGHGTEATMTHDQLLTDPEQAADFVKRTQLD
                     ALAIAIGTSHGAYKFTRKPTGDILAIGRVKEIHARIPNTHLVMHGSSSVPQDLLAEIR
                     KYGGDMKETYGVPVEEIQEAIKHGVRKINIDTDVRLAMTAAIRKFMVENPSKFDPREY
                     LKPAREAAKLICKQRYQQFGCEGQASKIKARPLVEVAQAYASGALAQTVN"
     gene            812..1753
                     /locus_tag="VFV25_12525"
     CDS             812..1753
                     /locus_tag="VFV25_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HEY1394191.1"
                     /translation="MAGAALQAGAHEEATAGPCRNCGAELASLAPPADFCPRCGQETA
                     QHPPTLGEFLHEFVGHYVALEGALWRTLGLLLLKPGRLTREYLAGRRRRYVLPLRLYL
                     TASFLFFLVLKLLPSAVNETEVLEAAARPGATVAEVQAAASAAARSTNPVIVLSNEQR
                     CGGVGEPACNAWQRLIRRVGERFRQDPRSFTEHMQAHMMGAAPYAIFLMLPAFAGIVM
                     LAYRSRRMTYGEHFVFSLHLHTFWFLALLAIELLPKGLGDLVQLAVPVYGVLALHEVY
                     RGRWGPTLARSLVISLLYGAVLLAAMAVLIGGLLAAA"
     gene            1779..>2158
                     /locus_tag="VFV25_12530"
     CDS             1779..>2158
                     /locus_tag="VFV25_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEY1394192.1"
                     /translation="MTSALLSSALHSLPLLARGKVRDNYAVGDDRILMIASDRLSAFD
                     VVMGEPIPGKGELLTQMALFWFDKLAGIVPNHLTGEAPESVVGANERAQVAGRSMLVK
                     RLKPIPVEAVVRGYLAGSGWKEYQ"
BASE COUNT          360 a          722 c          734 g          342 t
ORIGIN      
        1 agcccggacg tatgcaaggg cgcgatcgac ctgggctttt cgtcggtgat gatggacggc
       61 tcgttgcagg ccgacggcaa gaccatcgcc agctacgact acaacgtcga cgtgacgcgc
      121 aaggtggtcg acatggcgca caaactgggc gtcacggtcg aaggcgagct gggctgcctg
      181 ggctcgctcg agaccatgcg cggcgacaag gaagacgggc acggcaccga agcgacgatg
      241 acgcacgacc agctgctgac cgatcccgag caggcggccg acttcgtcaa gcgcacccag
      301 ctcgatgcgc tggcgatcgc aatcggcacc agccacggcg cctacaagtt cacgcgcaag
      361 cccaccggcg acatcctggc catcggccgc gtcaaggaga tccacgcgcg catccccaac
      421 acccacctgg tgatgcacgg cagttccagc gtgccgcagg acctgctggc cgagatccgc
      481 aagtacggcg gcgacatgaa ggagacctat ggcgtgccgg tcgaggagat ccaggaggcg
      541 atcaagcacg gcgtgcgcaa gatcaacatc gacaccgacg tgcgtctggc gatgacggcg
      601 gcgatccgca agttcatggt cgagaacccg agcaagttcg acccgcgcga atacctcaag
      661 ccggcgcgcg aggccgccaa gctcatctgc aagcagcgct accagcagtt cggctgcgag
      721 ggccaggcga gcaagatcaa ggccaggccg ctggtcgagg tcgcgcaggc ctatgcctcc
      781 ggggccctgg cgcagacggt gaactgaagc ggtggctggc gcggcgctgc aggcgggagc
      841 tcacgaggag gctaccgcag gtccttgtcg caattgcggc gccgagctgg cctcgctcgc
      901 gccgccggcc gacttctgcc cgcgctgcgg gcaggaaacc gcgcagcacc cgccgacgct
      961 gggcgagttc ctccacgagt tcgtcggcca ctatgtcgcg ctcgagggcg cgctgtggcg
     1021 cacgctgggc ctgctgctgc tcaagcccgg ccgcctgacg cgcgaatacc tcgcggggcg
     1081 ccgtcggcgc tatgtgctgc cgctgcggct ttatctcact gcgagcttcc tgttcttcct
     1141 ggtgctcaag ctgctgccgt cggcagtaaa cgagaccgag gtgctcgagg ccgcggcacg
     1201 cccgggcgcc acggttgccg aggtacaggc agcggccagc gcggcagcac gcagcaccaa
     1261 cccggtgatc gtgctcagca acgagcagcg ctgcggcggt gtgggcgagc cagcgtgcaa
     1321 cgcctggcag cgcctcatcc gtcgcgtcgg cgaacgcttc cgccaggacc cgcggagctt
     1381 taccgagcac atgcaggcac acatgatggg cgcggcacct tatgccatct ttctgatgct
     1441 gccggccttc gccggcatcg tgatgctggc ctaccgcagc cggcgcatga cctatggcga
     1501 gcatttcgtc ttcagcctgc atctgcacac gttctggttc ctcgcgctgc tagcaatcga
     1561 actgctgccg aaggggctgg gcgatctcgt ccagcttgca gtcccggtct acggcgtctt
     1621 ggcgctgcat gaggtctatc gaggccgctg ggggccgacg ttggcgcgct cgctcgtgat
     1681 cagcctgctc tacggcgcgg tgctgctggc ggcgatggcc gtgctgatcg gcggtctgct
     1741 ggcggcagcc tgagggggct gcctctacaa tcgccggcgt gacttctgca ttgctttcca
     1801 gcgccctcca ttccctgccc ttgctcgcgc gcggcaaggt gcgcgacaac tatgcggtcg
     1861 gtgacgatcg catcctgatg atcgcgagcg atcggctctc ggccttcgac gtcgtcatgg
     1921 gcgagcccat ccccggcaag ggcgagctgc tcacgcagat ggcgctgttc tggttcgaca
     1981 agctggccgg catcgtgccc aaccacctga ccggcgaagc gcccgaaagt gtggtcggcg
     2041 cgaacgagcg cgcgcaggtc gcgggccgct ccatgctggt caagcgcctg aagccgattc
     2101 ccgtcgaggc ggtggtgcgc ggctacctgg ccggcagcgg ctggaaggaa taccagga
//
LOCUS       DASUCY010000633         2589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_737177, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000633 DASUCY010000000
VERSION     DASUCY010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737177"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VFV25_12535"
     CDS             complement(<1..239)
                     /locus_tag="VFV25_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1394193.1"
                     /translation="MSCRTPVLHPLAAAVALAYFCATPGHAQQAPAPEAAASAPSGTT
                     SGAKLETVTVTAERRSESILDVPVSVSTLSGEKLDV"
     gene            complement(342..1397)
                     /locus_tag="VFV25_12540"
     CDS             complement(342..1397)
                     /locus_tag="VFV25_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010098704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside permease"
                     /protein_id="HEY1394194.1"
                     /translation="MNLQPTLLRLARALCTALAGLAATFANAQAADAPRPVKVLIITM
                     FGPEAAPWIEPFKLTQEIAVPGLSTDYPALKCNSDDVCLLTTGMGHANAAASTLAVAL
                     DPRFDLRQSYVLISGIAGIDPAQGTLGTAAWARWLIDFGIAHEIDARETPRHWKSGHF
                     GILTKGPGEKPKFDYRTEAFRLDEALLQQALALSRDVQLADSKEAKAYRAHYRQAPAS
                     EPPRVTQCDTLAGDTWFHGHTLGRQARDWAKLLSDGQASYCTTQQEDNATYNALARAT
                     AAGRADVKRLAVLRTASNFDRPYPGQTAYASLMASTSGATGGFVPATENLVRAGGPLV
                     RDIVARWALWKDGVPPR"
     gene            complement(1412..2458)
                     /locus_tag="VFV25_12545"
     CDS             complement(1412..2458)
                     /locus_tag="VFV25_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEY1394195.1"
                     /translation="MPFHRLRAAGRALTAALTLATALAVPSAHAAPRCLSECTPRIGI
                     VSAFGAEADILLAQTTAKREWSINGNRYTTGRLRGNRVVITLSGVSMINATMATQLML
                     DHFRIERLVMSGIAGGVNPARHVGDVLAPERWAMPMEVYWNADERIPAPCGKETDVSC
                     LGLRLAMDAAGKPWPDFRVNGSASPVSTGLFMRENFVMNSANAPQGEFRFDYPADARM
                     LAVARALQPALQRCGPKDATLCVSTTPELVVGGRGISGTAFLANPQYRRYLYDTLQAE
                     AVDMETAALGHVAYANRVPYIGFRSLSDLAGGDHTQDVGAFFGSGLAEANASAVTLAF
                     LEAWKKQAAGLAQH"
BASE COUNT          356 a          833 c          978 g          422 t
ORIGIN      
        1 acatccagct tctcgccgct cagcgtgctc accgagaccg gcacgtcgag gatgctctcg
       61 ctgcggcgct cggctgtcac cgtcacggtc tcgagtttgg ctcctgaagt ggtcccagaa
      121 ggggcggagg ccgcggcctc aggcgccggt gcctgttggg cgtggccggg cgtggcgcag
      181 aaataggcga gtgcgacggc cgccgccagc gggtgcagca cgggtgtccg acaggacatg
      241 ggaatccctc catatgtagg tatcgagggc catgtccgca attcccatgc cacacggcta
      301 tgccgcgtgg cagtgcgtgg gcgagccgag tgggtggctg gttagcgcgg cggcacgccg
      361 tctttccaca aggcccagcg cgccacgatg tcgcgcacca gcgggccgcc ggcgcgcacc
      421 aggttctcgg tggcgggcac gaagccgccg gtggcgccgc tggtggaggc catcagcgag
      481 gcataggccg tctgccccgg ataggggcgg tcgaagttcg aggcggtgcg cagcaccgcc
      541 aggcgcttca catcggcgcg gccggccgcg gtggcgcgcg ccagggcgtt gtaggtggcg
      601 ttgtcttcct gctgcgtggt gcagtagctg gcctggccgt cgctcagcag cttggcccag
      661 tcgcgcgcct gccgcccgag ggtgtggccg tggaaccagg tgtcgccggc cagcgtgtcg
      721 cactgggtca cgcgcggcgg ctcgctggct ggtgcctggc ggtaatgggc gcggtaggcc
      781 ttcgcctcct tgctgtccgc caactgcacg tcgcggctca aggccagcgc ctgctgcagc
      841 agggcttcgt cgagtcggaa ggcctcggtg cggtagtcga acttgggctt ctcgcctggg
      901 cctttggtga ggatgccgaa atggccgctc ttccagtgcc gcggcgtctc gcgcgcatcg
      961 atctcgtgcg cgataccgaa gtcgatcagc cagcgcgccc aggcggccgt gcccagcgtg
     1021 ccctgggccg ggtcgatgcc ggcgatgccg ctgatgagca cgtagctctg gcgcaggtcg
     1081 aagcgcggat ccagcgcaac ggccagcgtg gaggccgccg cgttggcgtg acccatgccg
     1141 gtggtgagca ggcacacatc atcgctgttg cacttgagcg ccgggtagtc ggtgctcagg
     1201 cccggcacgg cgatctcctg ggtgagcttg aagggctcga tccatggcgc ggcctcgggg
     1261 ccgaacatcg tgatgatcag caccttgacc gggcgcggcg cgtccgcggc ctgggcgttg
     1321 gcgaaggtgg ctgccaggcc ggcgagggcg gtgcacagcg ctcgggcgag gcgaaggagg
     1381 gtcggttgca ggttcatgag ggcatcgagg ttcagtgctg ggcaaggcct gcggcctgct
     1441 tcttccaggc ctccaggaag gccagcgtca ccgccgacgc attcgcctcg gcgaggccgc
     1501 tgccgaagaa ggcgccgacg tcttgcgtgt ggtcgccgcc ggcgaggtcg gacaggctgc
     1561 gaaagccgat gtagggcacg cggttcgcat aggccacgtg gccgagcgcg gccgtttcca
     1621 tatccaccgc ctcggcctgc agggtgtcgt agaggtagcg gcggtactgc gggttggcga
     1681 ggaaggcggt gcccgagatg ccgcgcccgc ccaccaccag ttcgggcgtg gtgctcacgc
     1741 acagcgtcgc gtccttgggg ccgcagcgct gcagcgccgg ctgcagcgcg cgtgccaccg
     1801 ccagcatgcg cgcgtctgcg ggatagtcga agcggaattc gccttgcggc gcgttggcgc
     1861 tgttcatcac gaagttctct cgcatgaaga gccccgtgga taccgggctt gcggagccgt
     1921 tgacgcgaaa gtcgggccag ggcttgccgg cggcatccat ggcgagcctg agcccgaggc
     1981 aggacacgtc ggtctccttg ccgcagggtg cgggaatgcg ttcgtcggcg ttccagtaca
     2041 cctccatcgg catggcccag cgctccggcg ccagcacgtc gcccacgtgg cgcgccggat
     2101 tcacgccgcc ggcgatgccg ctcatcacca accgctcgat gcggaaatgg tcgagcatca
     2161 gctgcgtggc catcgtggcg ttgatcatgc tgacgccgct cagcgtgatg acgacgcggt
     2221 tgccgcgcag cctgccggtg gtgtagcggt tgccgttgat gctccactcg cgcttggccg
     2281 tcgtctgcgc cagcaggatg tcggcctcgg cgccgaaggc cgagacgatg ccgatgcgcg
     2341 gcgtgcactc gctcagacag cggggagcgg cgtgggcgga tggaacggca agggcggtgg
     2401 ccagcgtcag tgcggccgtg agggcgcgcc cggcggcgcg aaggcgatga aagggcatga
     2461 gcggtcgtct ccctggtgtg aaagcgggtg cagcatgcca gcaatcgaca tgccagcggc
     2521 tgcctccgca gtggcggtct ggggagccct ttacgctggc ggtcggcccc ccggacttcg
     2581 ctgcacgtc
//
LOCUS       DASUCY010000634         2096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_962106, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000634 DASUCY010000000
VERSION     DASUCY010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962106"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VFV25_12550"
     CDS             complement(<1..425)
                     /locus_tag="VFV25_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HEY1394196.1"
                     /translation="MATEPAAQRVYRAACPNCGAPVEFRSAASPMAVCSFCRSTLLRE
                     GEALRRIGVSAELFDDHSPLQLGAGGKYQGAGFTLVGRLQMRYAEGSWNEWHAVFDSG
                     KSGWLSEDNAQFVFSFEAPAPGPLPNAEALRVGAQQVLGR"
     gene            complement(446..1489)
                     /locus_tag="VFV25_12555"
     CDS             complement(446..1489)
                     /locus_tag="VFV25_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEY1394197.1"
                     /translation="MALMDFIKKQFIDILEWVEDGDGTLAFRYPMADREIQYGGSLTV
                     RESQMAVFVNEGKLADVFGPGRYTLTTQTIPILTYLKNWDKLFESPFKSDVYFFSTRQ
                     QIDQRWGTTQPVTIRDKDFGAVRLRAFGNYAYRIADPKLFHTEISGTRDRYTLADLDG
                     QLRALMLQYISDAVAGSGLPFLDLAANQVAFAAQLQKAAAPAFEKLGLKLEAVTVQNV
                     SLPEELQKILDQKIGMGMVGGDMGKFMQYQTAQAVPTLAAGVGEGGGGGVMGSAVGLG
                     AGVALGQVMAQNLQQGLQGAAAASPAAAAPAGVRPDEVMATIEKLAELKAKGILTEEE
                     FNAKKAELLKKLA"
     gene            1634..>2096
                     /locus_tag="VFV25_12560"
     CDS             1634..>2096
                     /locus_tag="VFV25_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HEY1394198.1"
                     /translation="MKPSTELAIDLAVVGAGPVGLALALHAARALPEARISLFDARPA
                     ELDVSADPRTLALSLGSVQELERLGVWGAIAPASAAITSVHVSQQQPALMGLAPEPEV
                     LIRAIDEAVPQLGAVASYGAIVAPLQAAWLAAAAREPARLLSRFGTKVTALK"
BASE COUNT          312 a          705 c          733 g          346 t
ORIGIN      
        1 cgccccagca cctgctgcgc gccgacgcgc aacgcttcgg cgttaggcag cgggcccggc
       61 gcgggcgcct cgaagctgaa gacgaactgg gcgttgtcct cgctcagcca gccgctcttg
      121 ccgctgtcga agacggcgtg ccattcgttc cagctgccct cggcatagcg catctgcagc
      181 cggccgacga gcgtgaagcc cgcgccctgg tacttgccac cggcaccgag ctgcagcggg
      241 ctgtggtcat cgaagagctc ggcgctgacg ccgatgcgcc tgagcgcctc gccttcgcgg
      301 agcagcgtgc tgcggcagaa gctgcacacc gccatcggcg atgcggccga gcggaactcg
      361 accggcgcgc cgcagttagg gcaggccgcg cgatagacgc gctgtgcggc gggttcggtg
      421 gccatgccct tttagtcgtc tgggctcagg ccagtttttt cagcagctcc gccttcttgg
      481 cgttgaactc ttcctcggtc aggatgccct tggccttgag ctcggcgagc ttctcgatcg
      541 tcgccatgac ctcgtcgggc ctgacaccgg ctggagccgc agcggcaggc gacgcggcag
      601 cggcaccctg caagccttgc tgcaggttct gcgccatcac ctggcccagc gccacaccgg
      661 cgcccaggcc gaccgcgctg cccatcacgc cgccgccccc gccttcgccg acgccggccg
      721 ccagcgtcgg cacggcctgc gcggtctggt actgcatgaa cttgcccatg tcgccgccga
      781 ccatgcccat gccgatcttc tggtcgagga tcttctgcag ctcctcgggc agcgagacgt
      841 tctgcaccgt gacggcttcg agcttgagcc ccagcttctc gaaggcgggc gccgcggcct
      901 tttgcagctg cgcggcgaag gcgacctggt tggccgcgag atcgaggaag ggcaggccgc
      961 tgcccgccac cgcgtcggag atgtattgca gcatcagcgc gcgcagctgg ccgtccaggt
     1021 cggccagcgt gtagcggtcg cgggtgcccg agatctcggt gtggaagagc ttgggatcgg
     1081 cgatgcgata ggcgtagttg ccgaaggcgc gcaggcgcac ggcgccgaag tccttgtcgc
     1141 gtatcgtgac gggctgggtc gtgccccagc gctgatcgat ctgctggcgg gtgctgaaga
     1201 aatagacgtc gctcttgaag ggcgactcga acagcttgtc ccagttcttc aggtaggtga
     1261 ggatggggat ggtctgggtg gtcagcgtgt agcggccggg gccgaaaaca tcggccagct
     1321 tgccctcgtt gacgaacacg gccatctgcg attcgcgcac cgtgagcgag ccgccgtact
     1381 ggatctcgcg atcggccatc gggtagcgaa aagccagggt gccgtcgccg tcctcgaccc
     1441 attccaggat gtcgatgaac tgcttcttga tgaaatccat cagggccatg gcgtcctccg
     1501 tgaacggaaa tgtggcgagc gggatgatag aggagggatg cgacgcgacc atccccaaca
     1561 agtgtgagag tgcagcggaa atgctcaagg gtgagggcta aactccgcga gaacgcatgg
     1621 gaataccgct cgcatgaagc catcgaccga gctcgccatt gatctggccg tggtcggcgc
     1681 cggcccggtc ggcctggcgc tggccctgca tgcggctcgc gcgctgcccg aggcccgcat
     1741 cagcctgttc gatgcgcgcc cggccgagct cgacgtctcg gccgacccgc gcacgctggc
     1801 gctgtcgctg ggcagcgtgc aggagctgga gcgactgggc gtgtggggcg cgatcgcgcc
     1861 ggcgagcgcc gccatcacca gcgtgcatgt ctcgcagcag cagccggcgc tgatggggct
     1921 ggcacccgag cccgaggtgc tgatccgcgc catcgacgag gccgtgcccc agctcggcgc
     1981 ggtggcgagc tatggcgcca tcgtcgcgcc gctgcaggcg gcctggctcg ccgctgcggc
     2041 gcgcgagccg gcgcggctgc tcagccgttt cggcaccaag gtcacggcgc tcaagc
//
LOCUS       DASUCY010000635         2509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1299760, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000635 DASUCY010000000
VERSION     DASUCY010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1299760"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="VFV25_12565"
     CDS             complement(<1..805)
                     /locus_tag="VFV25_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEY1394199.1"
                     /translation="MFTINPDSPTPLVSQIVDGFRRLIANQSLKPGAKLPSIRSFAAS
                     HGVSVFTVVEAYDRLVAQGWLASRAHAGFFVKRRAGDAPLPAEALAPDVRFDARWYLK
                     QIFENRGLPVKAGCGWLPDDWLFEEGVRRSLRQLATDGAQIGGYGLPHGHMALRLLIA
                     ESLAEHQIAVGAEQVLLTQGSSQALDLLARRLVKPGDAVLVDAPGYPNLMFALRFLGA
                     RLIGVPRRPGGYDLAALESLMAEHEPKAFFTQPRLQSPTNSVTPLGQLYR"
     gene            946..2262
                     /gene="gabT"
                     /locus_tag="VFV25_12570"
     CDS             946..2262
                     /gene="gabT"
                     /locus_tag="VFV25_12570"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007827897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HEY1394200.1"
                     /translation="MTEPTMQRDAQAAQNTNAALMARRRAAVPRGVGQAHEIFIARGK
                     NAELWDVEGKRYIDFAGGIAVLNTGHCHPEVVAAVKAQLDLYTHTCFQVIAYEPYVEL
                     AERLNALAPGPFEKKTLFLSTGAEAVENAVKIARAYTRRSGLIAFTGGYHGRTLMTLG
                     LTGKVAPYKSGFGPFPGEVFHALFPNELHGVSVEDALQSVELIFKNDIEADRVAAFIV
                     EPVQGEGGFYVAPREFMTGLKRLADQHGILLIADEVQTGAGRTGTWFACEQWPVAPDL
                     IATAKSLAGGFPISGVVGRAEVMDAPGPGGLGGTYAGSPIGCAAALAVLKVFETEKLL
                     ARAQAVGERLIGGLQRIAASVPSIRDVRGLGAMVAVELFKDGDLTQPDAELTKRLVTE
                     AARRGLILLSCGSYGNVIRMLVPLTAADALLDEGLQILADSFHAVK"
     gene            2262..>2509
                     /locus_tag="VFV25_12575"
     CDS             2262..>2509
                     /locus_tag="VFV25_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY1394201.1"
                     /translation="MATSPAASSSASSSATSLATRPAPEPLVRFRGVQKTYDGVQLVV
                     RQLDLDIRHGEFLTLLGPSGSGKTTTLMMLAGFESPTAG"
BASE COUNT          421 a          863 c          826 g          399 t
ORIGIN      
        1 ctcggtagag ctgacccagc ggcgtcaccg aattggtcgg gctttgaagg cgcggttgag
       61 tgaagaaggc cttcggctcg tgctcggcca tcagcgactc cagcgccgcc aggtcgtagc
      121 ccccgggccg gcgcggcaca ccgatcaggc gcgccccaag aaagcgcagc gcgaacatca
      181 ggttcggata gcctggggca tcgaccagca ccgcgtcgcc cggcttcacc agtcggcgcg
      241 ccagcagatc cagcgcctgg ctcgagcctt gggtcagcag cacttgctcg gcgcccacgg
      301 cgatctgatg ctcggccagc gattcggcaa tcagcaagcg cagcgccatg tggccgtgcg
      361 gcagtccgta gccgccgatc tgggcgccgt cggtagccag ctggcgcagg ctgcggcgca
      421 cgccttcctc gaacagccaa tcgtcgggca gccaaccgca gccggccttg acgggcaggc
      481 ccctgttctc gaagatctgc ttcagatacc agcgcgcgtc gaagcgcacg tcgggcgcca
      541 gcgcttcggc cggcagcggc gcgtcgccgg cgcggcgctt gacgaagaag cccgcatggg
      601 cacgcgacgc cagccagccc tgagccacca gtcggtcata ggcctccaca acggtaaaca
      661 cgctgacgcc gtgcgaggcc gcgaagctgc ggatcgaggg cagcttggcg cccggcttga
      721 gcgactggtt ggcgatcagg cggcgaaagc cgtcgacgat ctggctgacc agcggcgtgg
      781 gggagtcggg attgatggtg aacatgcggc ggtcgatagc ggtgcggtac gcaccggatc
      841 agggcttcca tgtacaggac gaacgacctg cacagtacac catgcgcaaa gcctggctgt
      901 acatggtatg tgcccggccg cctgtctaaa gttgatgcgt tgacgatgac ggagccgacc
      961 atgcagcgcg atgcccaagc cgcccagaac accaatgccg cattgatggc acgccgccgt
     1021 gccgccgtgc cgcgcggcgt gggccaggct cacgagatat tcatcgcgcg cggcaagaac
     1081 gccgaactgt gggacgtcga aggcaagcgc tacatcgact tcgccggcgg cattgccgtg
     1141 ctcaacaccg gccactgtca tcctgaggtc gtcgccgccg tcaaggccca gctcgatctc
     1201 tacacccaca cctgcttcca ggtcatcgct tatgagcctt acgtggagct ggccgagcgc
     1261 ctgaacgcac tggctccggg gccgttcgaa aagaagacgc tgttcctgtc gaccggcgcc
     1321 gaggcggtcg agaatgccgt caagatcgcg cgcgcctaca cccgccgaag tgggttgatc
     1381 gccttcaccg gcggctacca cggccgcacg ctcatgacgc tcggcctgac cggcaaagtg
     1441 gcgccctaca aaagcggctt tggtccgttt ccaggggagg tgttccacgc gcttttcccc
     1501 aatgagttgc atggcgtaag cgtcgaggac gcgctgcagt cggtcgagct gatcttcaag
     1561 aacgacatcg aagccgaccg cgtggcggcc ttcatcgtcg agccggtgca aggcgaaggc
     1621 ggcttctacg tcgcgcccag ggaattcatg accggcctca aacgcttggc cgaccagcac
     1681 ggcatcctgc tcatcgccga cgaggtgcag accggcgccg gccgaaccgg aacctggttt
     1741 gcctgcgagc agtggccggt ggcgcccgat ctcatcgcca ctgccaaatc gctggccggt
     1801 ggcttcccca tctccggggt ggtgggccgc gccgaggtca tggacgcacc cgggcccggc
     1861 ggcttgggcg gcacctacgc aggctcgccg atcggctgcg cggccgccct cgccgtgctc
     1921 aaggtgttcg agaccgaaaa gctgctcgca cgcgcccagg cggtcggcga gcggctcatc
     1981 ggcgggctgc agcgcatcgc cgcctcggtg ccgtcgattc gcgatgtgcg cggtcttggg
     2041 gcgatggtgg cggtcgagct cttcaaggac ggcgacctca cccagcccga tgccgagctc
     2101 accaagcgcc tggtcaccga agccgctagg cgcggcctga tcctgctctc gtgcggcagc
     2161 tacggcaatg tgatccgcat gctggtgccg ctcacggccg cggatgcgct gctggacgaa
     2221 ggcctgcaga tcctggccga cagcttccac gcggtgaagt gatggcaacg agccccgcag
     2281 cctcttcttc ggcctcttcc tcggccacct ccctcgcaac tcggcccgca cccgaaccgc
     2341 tggtgcgctt tcgcggcgtg cagaagacct atgacggcgt gcagctggtc gtgcgccagc
     2401 tcgatctgga catccgccac ggcgagttcc ttaccctgct cggcccctcc ggttcaggca
     2461 agaccaccac gctcatgatg ctggccggct tcgagtcgcc cacagctgg
//
LOCUS       DASUCY010000636        10569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1315440, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000636 DASUCY010000000
VERSION     DASUCY010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 10569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10569
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1315440"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /gene="glk"
                     /locus_tag="VFV25_12580"
     CDS             complement(<1..861)
                     /gene="glk"
                     /locus_tag="VFV25_12580"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEY1394202.1"
                     /translation="MTPPLMFDGRLAQSFPWLVADIGGSNARFGWLSGPQAGVQHVRT
                     LPVAGHAHLADAVRAYLGGLASVLGDAFRPPRRAAFAVATAVAGDLVDLTNSHWSFSR
                     AALRDELGLDSLAVLNDFEALALALPRLDESQLRAFGPAAGAHQGTLAVIGPGTGLGV
                     AGVVSTRAGWVALAGEGGHATLSAADDFESELLARVRKHYEHVSAERLLSGIGLPVLH
                     SAVADVLALAAQPLSAEAIVDRGVAASDPVCVRTLDVFCALLGSFAGNVALTLGARGG
                     VYIGGGIVPRL"
     gene            1421..3343
                     /locus_tag="VFV25_12585"
     CDS             1421..3343
                     /locus_tag="VFV25_12585"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEY1394203.1"
                     /translation="MLPFDSPRLIADIGGTYARFALETAQGQFTHLASLRCAEHADFH
                     AAVKAYLASVPGTHIEHAAIAIANPVDGDRVQMTNYHWQFSIEEMRQQLGLETLVVVN
                     DFTALAMALPRLGSKDVRQIGGGEPVKQSVIGLLGSGTGLGVSGLIPTNGGWISLGSE
                     GGHTSFSPRDEREIAILRHAWQHFDHVSFERLLSGPGIELMYRALAERAQAPVEPLSA
                     PEITQRAVEGRDPVCTDTVDVFCSLLGTAAANLAVTLGAHGGIYVGGGIVPRLGAYFD
                     RSPFRARFEDKGRFRAYVAAIPTYVITAENATFIGASAILDAQLRALASAPGSAILEQ
                     IRRARSELSPAELRVAEQVLAHPRTVLNNPIADIARAAQVSQPTVIRFCRSMGCEGLS
                     DFKLRLASGLTGTVPLTHTQVTNDDSMLELGSKVLDNTASSILQVRSQLNREMIDRAI
                     ELLLRANRVEFYAVGHYGVVAQDAQFKFLRLGLSCAAYTDTRLQQLAAAVLQPGDVAV
                     IISAGGRLPELLDVVDKVHERGASVVAITASQSPLARKADATLVVDHVEDVSTHLPMV
                     SRILHLLVIDILSVGVAMRRDPESMAQLDGDATAGLDESDTASQPKRAAVAGARSAAP
                     GVSMASPLARLTSHSR"
     gene            3571..5268
                     /locus_tag="VFV25_12590"
     CDS             3571..5268
                     /locus_tag="VFV25_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY1394204.1"
                     /translation="MRLIRDLKIGTRLGGAFAAVLALLIAITGVAIVNLAKLNAGAEL
                     ITHTEFPKTELETSALDNARGSIARVFQIVTDDDKERSGKARERFQANVKIYADALAQ
                     LDARLTRPQARELLAKAKRSSDAYGASTAKVFALLDEDKREEAQRLAYADTYDALHAF
                     ANDLRALLDFGQKVVDDTGAESAKTYESTRNLMIALGLLAVALGAACAWLVTRSITRP
                     IAQAVAVAKTVASGDLSSKVEINSKDELGELLLALGHMNASLVAIVGNVRNAADSIAT
                     GSSQIATGNADLSQRTEEQASNLQQTAASMEQLTAAVKHNADTARQATQLAGDASSAA
                     EQGGQVVGRVVQTMGEIAASSKKIADIIGVIDGIAFQTNILALNAAVEAARAGEQGRG
                     FAVVAGEVRGLAQRSAQAAKEIKGLIGESVDKVESGYRLVDDAGRTMGGIVAQVKRVS
                     DLIGEISNASVEQSAGIGQVGDAVAQLDQVTQQNAALVEESAAAAESLKHQAGQLAQT
                     VAVFKLQARAEGAAPSGAHARPAAAPSGPGSTKLTPIKAQAEASLAEAPADGDWTSF"
     gene            5390..8281
                     /locus_tag="VFV25_12595"
     CDS             5390..8281
                     /locus_tag="VFV25_12595"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEY1394205.1"
                     /translation="MKLLSQRRAPLPAAAPPIAQSLLVWSTLLASFALVLSIVAAQLL
                     VYEPARRSLAAAELTDAADRVEAQLRTIVQRVEAIATLRRDWGSSGLIDLDRVPELVR
                     FLGPLLTRGPNVSSMAIANDAGREILLLHGSDGRYMTRHTDPDHLQGKAVMTTWLPGG
                     GLASAETVVSDYDARKRPWFIAAQAEKDPTAVVWTEPFVFRSTQMPGMSAVVRWTSPQ
                     GQRYTSTTDITLLDLSRFTGRLAVGRHGFATVLTPDGRVVGLPRDARFASEAALKSAV
                     LQHVPALGMPVLTAGYALWRANGLHGDSAERFEVAGTPWLGTFRRITLGSQVLWVGAF
                     APESDFAVTNAAQLTTLAAIVLFTLGLVWLVTRSIAKRFASPLQALAAESERIGRLEL
                     DEPVRVEAAWQEVAASAQAHEVMRTKLLQATRGLEAAVQQRTAELTQARDAAEAGTRA
                     KAMFLANMSHEIRTPMNAISGLTQLLQRTPLSGTQKDYVGKIEDAAALLLHIVNDVLD
                     YSKIEAGKLSVEHTEFMLDDLLRRVTQIIAPSAHERSLELVVRRAPQLPNVLVGDPVR
                     LEQILVNLAGNAVKFTLSGEVFIGAELLDQAGDSTRIRFTVRDSGIGMTEEQIQGLFQ
                     SFNQGDASMARRFGGTGLGLAISKKLVELMHGDIEVESRPGSGSSFRITLPLERAKGA
                     LETLARRAHSLAGMRALVVDDNETARMTLAEILRSFDMEVDVAGDGPSAIAAFGEACR
                     NGLAPQLVLVDWRMPVMDGFEVIQALRSEFPDAQTLFIMVTAAERLALEERITAERLA
                     GYVLKPATPSSLLDAIISSLGASAPAAPTSGAAARGAVPHAGQRVLIVEDNEVNRIVA
                     KELLKAFGVDAMTASSGGEAITRIRGGERFDLIFMDVQMGGMDGLQTTRILRSMPEGK
                     DLVIVALTAHAMPADRAHCIAAGMDDYVAKPISADDLAACLRRWLPAGP"
     gene            complement(8440..9429)
                     /locus_tag="VFV25_12600"
     CDS             complement(8440..9429)
                     /locus_tag="VFV25_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY1394206.1"
                     /translation="MNEARPVRLPTSREDTVAVVAFDGISPFHLSVPCLVFGEDRSDA
                     GVPRYRLLVCAAEHGPLRTTAGFQIEAPHGLKAIDKAGMVIVPSWRDDLATPPAALLQ
                     ALRRAHRRGARIVGLCLGAFVLAEAGLLDGLAATTHWHLAPAFAERYPQVRLEPMVLY
                     VDEGDIVTSAGTAAAIDCCLHLMRQQHGAEVANRAARRMVVAPHRQGGQAQYIERPVP
                     ESGADHRLTKVLAWITRHLDQPHSLDALAERAAMSRRSFTRHFRQATGTTVVKWLLNQ
                     RLARAQRLLETTDHSIDAVACEAGFGSAVSLRQHFGAALNTSPLAYRREFRGA"
     gene            9541..10173
                     /locus_tag="VFV25_12605"
     CDS             9541..10173
                     /locus_tag="VFV25_12605"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HEY1394207.1"
                     /translation="MSQAPSRALVVIDVQNEYFTGQLPIEYPPVEQTLPNIVRAMDAA
                     RAAGIPVVVVQHSAPAASPLFAKGSPGWQLHEEVGQRPRDHYIEKAWPSVYTGTDFAD
                     WLAAHDIDTLTVAGYMTHNCDASTIFEAMHHGLKVEFLADATGALPYANAAGRASAEE
                     IHRVFSVVFHSNFAAVASTRQWLDAIKDRVPLARDSVAASNRRARVDALV"
BASE COUNT         1791 a         3682 c         3546 g         1550 t
ORIGIN      
        1 cagccgcggc acgatgccgc cgccgatgta cacaccgccg cgcgcgccga gcgtcagggc
       61 cacattgccg gcgaagctgc ccagcagtgc gcagaacacg tcgagcgtcc ggacgcagac
      121 cgggtcgctg gcagccacgc cccggtcgac gatggcctcc gcgctcagcg gctgggccgc
      181 cagggcgagc acgtcggcca ccgcgctatg cagcaccggc aggccgatgc ccgacagcag
      241 ccgctccgcg gagacatgct cgtagtgctt gcgcacgcgg gccagcagtt cgctttcgaa
      301 gtcgtcggca gcggacaggg ttgcatgccc gccctcgccc gccagcgcca cccagccggc
      361 gcgggtcgat acgacgcccg ccacgcccag gcccgtgccg gggccgatga cggcgagcgt
      421 gccctgatgc gcgccggcgg ccggcccgaa ggcgcgcagc tgagactcgt ccaggcgcgg
      481 cagcgccagg gccagcgctt cgaaatcgtt gagcacggcc aggctatcga gccccagctc
      541 gtcgcgcagg gccgcgcgcg aaaagctcca atggctgttg gtcaggtcga cgaggtcgcc
      601 cgccaccgca gtggccactg cgaaggccgc gcgccgaggc ggacggaacg cgtcacccag
      661 cacggacgcg agccccccca gataggcacg cactgcatcc gccagatggg catgaccggc
      721 cacgggcagc gtgcgcacgt gctgcactcc ggcctgcggc ccgctcagcc aaccgaagcg
      781 cgcgttggag ccgccgatat cggcgaccag ccagggaaag gactgcgcga ggcgcccgtc
      841 gaacatcaag ggcggcgtca cgacaggcag cttctcttga ttcggatgcg tgagccgccg
      901 ccttcgcgga gcgttccggc ggcaatgtcc gccaaacgcg ggcctgtact gagtcgcacg
      961 tggtttcttc ctcgcctgtg tcctgctccg cccgcgtcac tgctgcgggc ccggccgacc
     1021 ccgcccaccc cggctgaatg acagcccctc cggcgatgct gaagtctcga atgccggaaa
     1081 ggtagcaaaa ctacatatca aagacaagag caagaacccc gctgcccact attccagccc
     1141 gcttcggcct tcgctcccca agaaatcctt gtgcatcctt gattgttacg gcgccacatc
     1201 gccaagatcc gcgccgccat cacgatggaa gctggacagt gtcatgtagt ttcactactg
     1261 atttggccgt tcatggtttc cgcgcctgcc ctcccggcac ccggcaggca aagaggcgaa
     1321 cgactccata atgggcattt cagtgcgcag cgcccactgg cggcctggct tcccgtcatt
     1381 ccgtcgctta cacggccctc ccgcatcagc agctgacgac atgcttcctt tcgacagccc
     1441 caggctcatc gccgacatcg gcggcaccta cgcccgtttc gcgctcgaaa cggcccaggg
     1501 gcagttcacc cacctcgcct ccctgcgctg cgcggagcac gccgacttcc atgccgcggt
     1561 gaaggcctac ctggcctcgg tgccgggcac gcacatcgag catgcggcga tcgcgatcgc
     1621 aaacccggtg gacggcgatc gggtgcagat gaccaactac cactggcagt tctcgatcga
     1681 ggagatgcgc cagcagctgg ggctggagac cctggtcgtc gtcaacgact tcaccgcgct
     1741 ggcgatggcc ctgcctcgcc tgggcagcaa ggacgtgcgc cagatcggcg gcggcgagcc
     1801 ggtcaagcag agcgtgatcg gcctgctggg ctcgggcacc ggcctgggcg tgtcggggct
     1861 gatcccgacc aatggcggct ggatctcgct gggctccgag ggcggccaca cgagcttctc
     1921 gccgcgcgac gagcgcgaga tcgcgatcct gcgccatgcc tggcagcatt tcgaccatgt
     1981 gtccttcgag cgcctgctgt ccggccccgg catcgagctg atgtaccggg ccctggccga
     2041 gcgcgcgcag gcgccggtcg agcctttgtc cgcacccgag atcacgcagc gcgcggtcga
     2101 ggggcgcgac ccggtgtgca ccgacaccgt cgacgtgttc tgcagcctct tgggcacggc
     2161 ggccgccaat ctggcggtga cgctgggcgc gcacggcggc atctatgtcg gcggcggcat
     2221 cgtgccgcgg ctgggtgctt atttcgaccg ctcgccgttt cgcgcccgct tcgaggacaa
     2281 ggggcggttc agggcctatg tggccgccat cccgacctat gtcatcacgg ccgagaacgc
     2341 gaccttcatc ggcgcctcgg ccatcctgga tgcgcagctg cgcgcactcg cgtccgcccc
     2401 cggctcggcc atcctcgaac agatccgccg cgcgcgcagc gagctgtcgc cggccgagct
     2461 gcgcgtggcc gagcaggtgc tggcgcatcc gcgcacggtg ctcaacaacc cgatcgccga
     2521 catcgcccgc gctgcccagg tcagccaacc gacggtgatc cgcttctgcc gctcgatggg
     2581 ctgcgagggc ctgtccgact tcaagctgcg cctggcctcc ggcctcaccg gcaccgtgcc
     2641 gctgacgcac acccaggtga cgaacgacga ctccatgctg gagctcggct ccaaggtgct
     2701 ggacaacacg gcctcctcga tcctgcaggt gcgcagccag ctcaaccgcg agatgatcga
     2761 tcgcgccatc gagctgctgc tacgagcgaa tcgggtcgag ttctatgcgg tcggccatta
     2821 cggcgtcgtg gcccaggacg cgcagttcaa gttcctgcgc ctggggctgt cctgcgcggc
     2881 ctataccgac acgcgcctgc agcagctggc ggccgccgtg ctgcagccgg gcgacgtggc
     2941 cgtgatcatc agcgccggcg ggcggctgcc cgagctgctg gatgtcgtcg acaaggtgca
     3001 tgaacgcggc gcctcggtgg tcgccatcac ggccagccag tcaccgctgg cgcgcaaggc
     3061 cgacgcgacg ctggtcgtcg atcatgtcga agacgtgtcg acccacctgc ccatggtgag
     3121 ccgcatcctg cacctgttgg tgatcgacat cctctcggtg ggcgtcgcca tgcgccgcga
     3181 tcccgagagc atggcgcagc tcgatggcga cgcgacagcc ggcctggatg agtccgacac
     3241 cgcgagccag ccgaagcggg ccgcagttgc cggcgcacgc agcgcggcgc ccggcgtcag
     3301 catggcctcg ccgttggcgc ggctgacctc gcacagccgc tgacgctctg ggcgctcggc
     3361 gcagcaatcc cttcacaact ctcgccgccg ccgggcaggg ttcggttgcg ggctgggccc
     3421 gcttgcgcat tgcgggcgcg atcgcagcgg tgctgtcggc agaagccgcc gcaactgaac
     3481 gaaaagtcca tggcttttcg agctttcgga gcgactgcgt cggccgaaaa tccgccaggg
     3541 gagccgaaga agttgtggct ggagcagtgc gtgagactta tcagagacct gaaaatcggc
     3601 acccgactgg gcggcgcttt cgccgcagtg ctggcgctgc tgatcgcgat caccggcgtg
     3661 gccatcgtga acctcgccaa gctgaatgcc ggcgccgagc tgatcaccca cacggaattc
     3721 cccaagaccg aactcgagac ctcggcgctg gacaacgcgc gcggcagcat cgcgcgcgtg
     3781 ttccagatcg tgaccgacga cgacaaagag cgctcaggca aggcgcggga acgcttccag
     3841 gccaacgtca agatctatgc cgacgccctc gcccagctcg acgcacgcct cacccggccc
     3901 caggcccgcg agctgctggc caaggccaag cgatcgagcg acgcctacgg tgcctcgacc
     3961 gccaaggtct tcgccctgct ggacgaggac aagcgcgaag aggcccagcg gctggcctac
     4021 gcagacacct atgacgccct gcatgccttc gcgaacgatc tgcgcgccct gctcgatttc
     4081 gggcagaaag tcgtggacga cactggtgcg gaaagcgcca agacttacga atcgacgcgc
     4141 aatctgatga ttgcgctggg actgctggcg gtggcgctgg gcgccgcctg cgcctggctg
     4201 gtcacgcgct cgatcacgcg ccccattgcc caggccgttg cggtggccaa gacggtggcc
     4261 agcggcgacc tcagctccaa ggtcgaaatc aactcgaagg acgaactcgg cgagctgctg
     4321 ctcgcgctgg gccacatgaa cgcgagcctg gtcgcgatcg tcggcaacgt gcgcaatgcc
     4381 gcggattcga tcgccaccgg ctcatcgcag atcgccacag gcaacgcgga cctgagccag
     4441 cgcaccgagg agcaagcttc gaacctgcag cagacggcgg cctccatgga gcagctcacg
     4501 gccgcggtca agcacaacgc cgacacggcg cgccaggcta cgcagctggc cggtgacgca
     4561 tcctcggccg cggagcaagg cggccaggtc gtcggtcggg tcgtgcagac catgggcgag
     4621 atcgccgcca gctcgaagaa gatcgccgac atcatcggcg tgatcgacgg cattgccttc
     4681 cagaccaaca tcctcgcctt gaacgcagca gtggaggcgg cgcgcgcggg cgagcagggc
     4741 cgaggctttg cggtggtggc cggcgaggtg cgcggcctgg cgcagcgcag cgcccaggcg
     4801 gccaaggaga tcaaggggct catcggcgag agcgtcgaca aggtcgaaag cggctaccgg
     4861 ctggtcgacg atgccggacg cacgatgggc gggatcgtcg cgcaggtcaa gcgcgtgtcc
     4921 gacctgatcg gcgagatcag caacgccagc gtggagcaaa gcgccggcat cggccaggtc
     4981 ggcgatgccg tggcgcagct cgatcaggtg acgcagcaga acgccgcgct ggtcgaagag
     5041 agcgcggcgg cagcggaaag cctcaagcac caggccggcc agttggcaca gacggtagcc
     5101 gtgttcaagc tgcaagcccg cgcagaagga gcggcaccgt ccggcgccca tgcgcgtcct
     5161 gcagcggctc ccagcgggcc gggctcgacg aaattgacgc caatcaaggc gcaggccgag
     5221 gcctccctag cggaagcgcc ggctgacggc gactggacga gcttctgatg aaaggctgtt
     5281 gtcaaaccgc gggcgaaggc cggtatctcg ttcggtggag aatccacgcc gaggctgccg
     5341 agtcgcgagc gcatcactgc atcgccagac accaaccccc cggcactgca tgaagctcct
     5401 gagccagcgc cgcgcccctc ttccagcggc cgcaccgccg atcgcgcaga gcctgctcgt
     5461 ctggtcgacg ctgctcgcga gcttcgctct cgtcctctcg atcgtcgctg ctcaactgct
     5521 ggtgtacgaa ccggcacgcc gctcgctggc cgccgccgag ctgaccgacg cggcagaccg
     5581 ggtcgaggcg cagttacgca cgatcgtcca gcgcgtcgaa gccatcgcga cgctgcgccg
     5641 cgactggggc agcagtggac tgatcgacct ggatcgcgtt ccggagctgg tgcgcttcct
     5701 gggaccgctg ctgacgcgcg gacccaatgt gtcgtccatg gccattgcca acgatgcggg
     5761 acgggaaatc ctgctgctgc acggatcgga tggccgctac atgactcgcc acaccgaccc
     5821 cgatcacctg caaggcaagg cggtgatgac gacctggctg ccgggaggcg ggctcgccag
     5881 cgcggaaacg gttgtcagcg actacgacgc ccgcaagcgg ccgtggttca tagccgcaca
     5941 ggcggagaaa gaccccaccg cagtggtctg gaccgagccc ttcgtgttcc gctcgaccca
     6001 gatgcccggc atgtcggcgg tcgtgcgctg gacttcgccg cagggccagc gctataccag
     6061 caccaccgac attactctgc tcgacctgtc gcgcttcaca ggccgtcttg cagtggggcg
     6121 gcacggcttc gccacggtgc tcacgcccga cggccgggtc gtcggcctgc ctcgcgacgc
     6181 acgcttcgca tccgaagcgg ccctgaagtc ggccgtgctc cagcacgtgc cggcactggg
     6241 catgcctgtg ctcaccgccg gctacgcgct gtggcgggcc aacggcctgc atggcgacag
     6301 cgccgagcgc ttcgaggttg caggcacgcc ctggctgggg acctttcgcc gcatcacgct
     6361 gggcagccag gtgctgtggg tcggcgcctt cgcgcccgaa tccgactttg ccgtgaccaa
     6421 cgcggcgcaa ttgaccacgc tggcggccat cgtgctgttc acgctcggcc tggtgtggct
     6481 tgtgacccgc agcatcgcaa agcgcttcgc ttcgcccctg caggccttgg ctgccgagag
     6541 cgagcgcatc ggccggttgg agctcgacga acccgtccgg gtggaagccg cctggcagga
     6601 ggtggccgcc agcgctcaag cccacgaggt gatgcgcacg aaactgctgc aagccacgcg
     6661 cggtctcgag gctgccgttc aacagcgcac cgcggagctg acgcaggcac gcgatgccgc
     6721 ggaagcgggc acgcgcgcca aggcaatgtt tctggccaac atgagccatg agatacgcac
     6781 ccccatgaat gcgatctcgg ggctaacgca gctgctgcag agaacgccgc tgtcgggcac
     6841 gcagaaggac tatgtgggca agatcgaaga cgccgcggca ctcctgctgc acatcgtcaa
     6901 cgacgtcctg gactattcga agatcgaggc gggcaagctc tccgtcgagc acaccgagtt
     6961 catgctcgac gacctgctgc gcagggtcac gcagatcatc gccccgtcgg cccacgaaag
     7021 gagcctggag ctggtggtgc gtcgcgcccc gcagctgccc aatgtgctgg tgggcgaccc
     7081 ggtgcggctg gagcagatcc tggtcaacct cgccggcaat gcggtgaagt tcacgctgtc
     7141 cggcgaggtg ttcatcggag ccgagctgct ggaccaggca ggcgactcga cgcggatccg
     7201 gttcacggtg cgcgactccg gcatcggcat gaccgaggag cagatccagg gcctcttcca
     7261 gtcgttcaac cagggtgatg catcgatggc ccggcgcttc gggggcacgg gcctgggcct
     7321 ggccatcagc aagaagctcg tcgagctgat gcacggtgac atcgaggtcg agagccggcc
     7381 cggctcgggc agcagcttcc ggatcacgct cccgctggag cgcgccaagg gcgctctgga
     7441 aacgctggcc agacgcgccc actcgctggc gggaatgcgc gcgctggtcg tcgacgacaa
     7501 cgagaccgcg cgcatgacct tggccgaaat cctgcgctcc ttcgacatgg aggtcgatgt
     7561 cgcgggcgac ggtccatcgg ccatcgccgc cttcggcgag gcctgccgga acggcctggc
     7621 gccgcaactg gtcctggtgg actggcgcat gccggtgatg gacggcttcg aggtcatcca
     7681 ggccctgcgg tcggaattcc cggatgcgca aaccctgttc atcatggtga ccgccgccga
     7741 gcggctcgct ctggaggaac gcatcaccgc cgagcgcctg gcgggctacg tgctcaagcc
     7801 cgcgacaccg tcctccttgc tggacgccat catctccagc ctgggggcat ccgcacccgc
     7861 agcgccgaca tccggcgcag ccgcgagggg ggccgtcccg catgcagggc agcgcgtcct
     7921 gatcgtggaa gacaacgagg tgaacaggat agtcgcgaag gaattgctca aggccttcgg
     7981 cgtggacgcc atgacggcct ccagcggcgg cgaggccatc acccggatac ggggaggcga
     8041 gcgcttcgac ctgatcttca tggacgtcca gatgggaggc atggatggcc tgcagacgac
     8101 gcgcatcctg aggtccatgc ccgagggcaa ggacctggtc atcgtcgcgc tgacggccca
     8161 cgcgatgccc gccgaccgcg cgcattgcat cgccgccggc atggacgact acgtcgccaa
     8221 gccgatatcg gcggatgatc tggcggcctg cctgcggcgc tggctgccgg ccggcccata
     8281 ggccgccgcg agtcatatca gttgctccac cgcgcccaga cgcggcccgc gacgcatgaa
     8341 ccagcgcaac caagcgcacg ccgcggatcc gcacttgccg gtccgccagc gtggccccct
     8401 cgaagggggc gcgcgtcagc gcgtagggga cggttcactt caagcgccgc ggaactcccg
     8461 ccgataggcg agcggtgatg tgttgagcgc cgcgccgaaa tgctggcgca gcgagaccgc
     8521 cgagccgaag cccgcctcgc aggcgacggc atcgatggag tggtcggtgg tctcgagcag
     8581 gcgctgcgcg cgcgccaggc gctggttaag cagccacttc accaccgtcg tgccggtggc
     8641 ctggcggaaa tggcgcgtga agctgcgccg gctcatcgcg gcgcgctcgg ctagcgcgtc
     8701 caggctgtgc ggctggtcga ggtgccgggt gatccaggcg agcaccttgg tcagccgatg
     8761 gtcggcgccg gactccggca cgggccgctc gatgtactgc gcctgcccgc cctggcggtg
     8821 cggcgccacc accatgcggc gcgcggcgcg attggcaacc tcggcgccgt gctgctggcg
     8881 catcaggtgc aggcagcagt cgatggcggc ggcggtgccg gccgaggtga cgatgtcgcc
     8941 ttcgtcgacg tacagcacca tgggctccag ccgcacctgg gggtagcgct cggcgaaggc
     9001 cggcgccaga tgccaatgcg tggtggccgc caggccatcc agcaggccgg cctcggccag
     9061 cacgaaggcg cccaggcaca ggccgacgat gcgcgcgcca cggcgatgag ccctgcgcaa
     9121 agcctgcaac agtgctgcgg ggggcgtcgc gagatcgtcg cgccacgagg gcacgatcac
     9181 catgccggcc ttgtcgatgg ctttcaggcc gtgcggcgcc tcgatctgga aaccggccgt
     9241 ggtgcgcaag ggcccatgct cggccgcgca caccagcagc cggtagcgcg gcacgcccgc
     9301 atcgctgcgg tcctcgccga acaccaggca gggcacggac agatggaagg ggctgatgcc
     9361 gtcgaatgcg accaccgcaa ccgtgtcttc gcggctcgtg gggagtcgga caggccgggc
     9421 ttcattcatg gcccgattct atcgaatatt gtcacttggg ccacttccgc gctccgccgc
     9481 tcacccgaac aattccagcc atggacggcg cagtgccgtc tgtcgcaaag gagaacccag
     9541 atgtcacaag cccctagccg cgcgctcgtc gtgatcgatg tgcagaacga atacttcact
     9601 ggccagttgc cgatcgagta cccgccggtg gagcagacgc tgcccaacat agtccgcgcg
     9661 atggatgccg ctcgcgccgc cggcatcccg gtggtggtgg tgcaacacag cgcgccggcc
     9721 gcctcgccgc tcttcgccaa gggctcgccg ggttggcagc tccacgagga agtgggacag
     9781 cgcccgcgcg accactacat cgagaaggcc tggcccagcg tctacaccgg caccgacttc
     9841 gccgactggc tggccgccca cgacatcgac accctcacgg tggcgggcta catgacgcac
     9901 aactgcgatg catccaccat ctttgaggcc atgcaccacg gtctgaaggt ggagttcctc
     9961 gcggatgcca ccggtgccct gccctatgcc aacgccgccg gtcgcgcaag cgccgaggag
    10021 atccatcgcg tgttcagcgt ggtgttccac agtaacttcg ccgccgtggc cagcacgcgg
    10081 caatggctgg acgcgatcaa ggaccgcgtg ccgctggcgc gcgacagcgt ggctgcctcc
    10141 aaccggcgcg cccgtgtcga tgcgctggtc tagggcgtcc tggacagcag catgcggaca
    10201 ccggcatagc cgaagacgac cgccagcgtc ccctcggaga cccgccgcct tgaaggccag
    10261 ccagagcaag taagcgcctg cgatcaaact tgaccacgag caaggcgccc gaccaggctg
    10321 ccagagcgcc ctgaacagtc gaggacggga cacaaaaata ctgtgtcgcg ccactgggat
    10381 gtaaggcttc cttcagtttg gatcacaatg ctgcatagca gcatgagcgc aaccaaacca
    10441 cggctgagtg ccgatctcgc aacggaactc gaggacatcc tcgggagcct gcgctttgcc
    10501 cggcgaacgg aagatctcgg tcgcctcgcg cttctgacct actgggaagt ccgccgctgg
    10561 gcgcgcacg
//
LOCUS       DASUCY010000637         3278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1508660, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000637 DASUCY010000000
VERSION     DASUCY010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1508660"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            268..882
                     /locus_tag="VFV25_12610"
     CDS             268..882
                     /locus_tag="VFV25_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006026571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like protein"
                     /protein_id="HEY1394208.1"
                     /translation="MQAAEHHHLIDVELGKLRPTQITVGFAEVTKKRAEWAQLSKKAR
                     RELLDSHWFPAVLGPRQRYHIVDHHHLGLALIREGVKRVRLMVLADLSYLVPGIFWRV
                     MEQHGWAHPFDHAGERRNYDDIPTRIPQLVDDPYRSLAGFLRASGGYAKDTSPFAEFL
                     WADYLRQHIAREQIEQSIDTAVREALGLAKSPLARYLPGWAGAH"
     gene            882..2528
                     /locus_tag="VFV25_12615"
     CDS             882..2528
                     /locus_tag="VFV25_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEY1394209.1"
                     /translation="MDAGVDEDGARRGGSVWLADLIAGVSMAGLLLPEAVAYSGIASM
                     PPQAGVIGLFAGLLVYGLIGRSRFAIVSATSSSAAVLGAATLSMAGADAALRATLAAG
                     LVLATGGAFLLAGTARLGTICNFISKPVLRGFAFGLALSIVIKQLPKMVGVHPQAGEL
                     LPFSVQLLLQAAHWNWAGVALGLGALLALRLLARFKKLPGALLVIAVGIAIGQLLDVK
                     AHGIDLVGNIALSLAVPALPELTRSEWLRLGELSLALLLLLFAESYGAIRSMALRHGD
                     EVTPNRDLLAFGGANLLSGLFHGMPVGAGYSATSANEAAGAQSGKSAWVAALVVLLLV
                     LAFLPWIERTPEPVLAAVVIHAVSHTLSLVVFRPYFNWRRDRFVVVAAALAVVVLGVL
                     DGLLAGIAISLAMMLRRLSQPRVSELGRLAGGHDFLSCEAHPDVHRIAGLLVLRPEEP
                     LFFANVERVLADVRARVAEAKPSGLVLSLEETPDLDGTSIEALSEFATDQARAGVALV
                     LARLKDPVLALLKGSTVGMGQAVLEPGSVDDAVGRLLPPE"
     gene            2757..>3278
                     /locus_tag="VFV25_12620"
     CDS             2757..>3278
                     /locus_tag="VFV25_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY1394210.1"
                     /translation="MSGKVLLETHELSKEFKGFVAVSKVNLRVQEGTIHALIGPNGAG
                     KTTCFNLLTKFLTPSSGRIVFDGADITAESPAHIARRGVIRSFQISAVFPHLSVLENV
                     RVALQRKLGTSYHFWRSERSLHQLNDEAIALLREVDLDKFAALPAVELSYGRKRALEI
                     ATTLAMDPKLMLLD"
BASE COUNT          472 a         1088 c         1156 g          562 t
ORIGIN      
        1 gaagggctgc tcgaattcgg ccgccgcggc agctgcgatg ccgctgaaag cggccaggcc
       61 ggtgccggcg aggctgccgg ccgccgccgc gacgagcttg cggcgcatgg agtcggtgct
      121 ggacatggcg ttctcgcttg ctgggttgct ggggtcgggg agcaactgtg cgccgccggc
      181 cgccggcgat gcgcctagaa caaaccctag cccaggcgtg gcagcgcggg catactcgct
      241 cgctcatcag cgcagaagga ggcggccatg caagcggcgg agcaccatca tctgatcgat
      301 gtcgagctgg gcaagctcag gcccacgcag atcaccgtgg gttttgccga ggtgaccaag
      361 aagcgcgccg aatgggcgca gttgagcaag aaggccaggc gcgagctgct tgattcgcac
      421 tggttccccg ccgtgctggg gccgcgccag cgctatcaca tcgtcgacca ccatcacctg
      481 ggtctggcgc tgatccgcga gggcgtcaag cgggtcaggc tgatggtgct ggcggacttg
      541 tcctatctgg tgccggggat cttctggcgc gtgatggagc agcacggctg ggcccatccc
      601 ttcgaccatg ccggcgagcg gcgcaactac gacgacattc ccacgcgcat cccccagctc
      661 gtcgacgatc cctaccgcag cctggccggc ttcctgcgcg cctcaggcgg ctatgccaag
      721 gacacctcgc cgtttgccga gttcctgtgg gccgactatt tgcgccagca catcgcccgc
      781 gagcagatcg agcaatccat cgacaccgca gtgcgcgagg ccctgggcct ggccaagagt
      841 ccgctggctc gctacttgcc tggctgggcc ggggcccact aatggatgca ggggtcgacg
      901 aggacggcgc gcgccgcggc ggctcggtct ggctggccga tctgatcgcc ggcgtttcga
      961 tggccgggtt gctgctgccc gaggcggtgg cctattccgg catcgccagc atgccgccgc
     1021 aggcgggggt gatcggcctc ttcgccgggc tgctggtcta tggcttgatc gggagatcgc
     1081 gcttcgccat cgtctcggcc acctcgtcct cggcggccgt gctgggcgcc gcgacgctga
     1141 gcatggccgg ggcggatgcg gcgctgcgcg cgaccttggc cgcgggcctg gtgctcgcca
     1201 cgggaggtgc cttcctgctc gccggcacgg cgcggctcgg cacgatctgc aacttcattt
     1261 ccaagccagt gctgcgcggc ttcgccttcg ggctggcctt gtccatcgtc atcaagcagc
     1321 tgcccaagat ggtaggcgtg cacccccagg ccggcgagct gctgcccttc agcgtccagc
     1381 tgctcttgca agccgcgcac tggaactggg ccggcgtggc gctggggctg ggcgccctgc
     1441 tggcgctcag gctgctggcg cgcttcaaga agctgccggg ggcgctgttg gtgatcgcgg
     1501 tgggcatcgc gatcggccag ctcctggatg tcaaggcgca tggcatcgat ctggtcggca
     1561 acatcgcctt gtcgctggcc gtgccggcgc tgcccgagct gacgcgcagc gaatggctgc
     1621 gcctgggcga gttgtcgctg gccttgctgc tgctgctgtt cgccgagagc tatggggcca
     1681 tacgcagcat ggcgctgcgg catggcgacg aggtcacgcc caaccgcgac ctgctggcct
     1741 tcggcggcgc caatctgctg tccggcctgt tccacggcat gccggtgggc gcgggctatt
     1801 cggccacctc ggccaacgag gcggcggggg cgcaaagcgg caagtcggcc tgggtggcgg
     1861 cgctggtcgt gctgctgctg gtgctggcct tcctgccctg gatcgagcgc acgccggagc
     1921 cggtgctggc cgccgtcgtc atccacgccg tttcgcatac gctgtcgctg gtcgtgttcc
     1981 ggccctattt caattggcgg cgcgaccgct tcgtcgtcgt cgcggcggcg ctggcggtgg
     2041 tcgtgctggg cgtgctcgac ggcctgctgg ccggcatcgc gatcagcctg gcgatgatgc
     2101 tcaggcgcct ttcgcagccg cgggtgagcg aactgggccg cctggcaggc gggcacgact
     2161 tcctgagttg cgaggctcac ccggacgtgc atcgcattgc cggcctgctc gtgctgcgcc
     2221 ccgaggagcc gctgttcttc gcgaatgtcg agcgtgtgct ggccgatgtc cgcgcgcgcg
     2281 tggccgaggc caagccgagc ggcctggtgc tgagcctgga ggaaacgccg gatctggacg
     2341 gcacctcgat cgaggcgctg agcgaattcg ccaccgacca ggcgcgcgcc ggcgttgcgc
     2401 tggtgctggc gcggctgaag gatcccgtgc tggccttgct gaagggctcg acggtcggca
     2461 tgggccaggc cgtgctggag ccgggcagcg tcgacgatgc ggtgggccgc ttgctgccac
     2521 cggaataagt catgcagatg gccgagcgca ccggataagt aagcttacct agtgcaaagc
     2581 ctgacgcccg ccgcgagcga acttggctag gatgccgctt ctatcggcga ggagctgggt
     2641 cttgcttgag cgagcctctc gacgatctgg gtcttaggga agagcaacaa ggctcgcgac
     2701 ccaccccttg ctacagactc cgaccaagca acaacgagcg tctgccaaaa cacagcatga
     2761 gcgggaaagt cctcctcgaa acgcacgagc tcagcaagga gttcaagggc ttcgtggccg
     2821 tcagcaaggt caatctgcgc gtgcaggagg gcaccatcca tgcgctgatc gggccgaacg
     2881 gcgccggcaa gacgacctgc ttcaatctgc tcaccaagtt cctgacgccg agctcgggcc
     2941 gtatcgtctt cgacggcgcc gacatcaccg cggaatcgcc ggcccacatc gcgcgccgcg
     3001 gcgtcatccg ctccttccag atctcggccg tgttcccgca cttgtcggtg ctggagaacg
     3061 tgcgcgtcgc gctgcagcgc aagctgggca cgagctatca cttctggcgc agcgagcgca
     3121 gcctgcacca gctcaacgac gaggcgatcg cgctgctgcg cgaggtcgac ctcgacaagt
     3181 tcgcggcgct gcccgcggtg gagctgagct acggccgcaa gcgcgcgctg gagatcgcca
     3241 ccacgctggc gatggacccc aagctgatgc tgctcgac
//
LOCUS       DASUCY010000638         2374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_399785, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000638 DASUCY010000000
VERSION     DASUCY010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399785"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="VFV25_12625"
     CDS             complement(<1..713)
                     /locus_tag="VFV25_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEY1394211.1"
                     /translation="MPRRLLGLAGIGVALVLAGCASSLHRAPVEDRSNSAARAASPTS
                     NAGAELPSAEVKPPPPGAENAGKPGYYTVKPGDTLIRIGLETGQNWRDIGKWNNIDNP
                     NLIEVGQVVRVLPPGADPNTAAARGVGSGNKLEGRPLDAKPGNAASAPAAVAQANPPA
                     QPASGADDDVSWVWPAPGPVIAGFDEARNKGVSISGKAGDPVYAAADGRVVYAGSGLR
                     GYGNLVIIKHNSTYLTAYA"
     gene            complement(840..1709)
                     /locus_tag="VFV25_12630"
     CDS             complement(840..1709)
                     /locus_tag="VFV25_12630"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEY1394212.1"
                     /translation="MSGDAESDRASKFPLRLDSLTKPAQRASSTHKAAQPIAPATPQS
                     TLRPQRPVHEAVQERARRATPAGLGLDSEGVRQRMVERLRAQGLAGVQVLAAMAAVPR
                     HLFVDTALANQAYEDTSLPIGLSQTISKPSVVARMIDLMLAGRTAPLGRVLEVGTGCG
                     YQAAVLARLARQVISVERLRPLHDKAREHLAQLPPLGLRNDGLRLVYGDGMLGHAPNA
                     PYDGIIAAAGGGALPAAWLDQLGPGGRLVAPMHDAASGGHVLVVVDRDLAGNLQRSFH
                     EAVHFVPLKSGVN"
     gene            complement(1706..>2374)
                     /gene="surE"
                     /locus_tag="VFV25_12635"
     CDS             complement(1706..>2374)
                     /gene="surE"
                     /locus_tag="VFV25_12635"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041929570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEY1394213.1"
                     /translation="PEQNASGTSNSLTLHRPLSVYAAGNGFRYVNGTPSDCVHVALTG
                     LLGYRPDLVVSGINNGANMGDDTLYSGTVAAAMEGYLFGVPAIAFSLVEKGWQHLDTA
                     ARVARSMIEQVIAMPPAGGAWLLNVNIPSRPYSELAGHRITRLGRRHASEPVIRQTNP
                     RGEAIYWIGPAGDAREAGEGTDFHAAAQGCVSVTPLQVDLTDHRALPAWGAWLEGAAS
                     EVQG"
BASE COUNT          379 a          806 c          819 g          370 t
ORIGIN      
        1 tgagcatacg ccgttaagta ggtgctgttg tgcttgatga tgacgagatt tccgtaaccg
       61 cgcagcccgg agccggcata gacgacgcgg ccatccgccg ctgcgtagac cgggtcgccg
      121 gccttgccgg aaatggacac acccttgttg cgcgcctcgt cgaagccggc gatcaccggg
      181 ccgggggccg gccagaccca gctcacgtcg tcgtcggcgc cgctggccgg ctgcgcgggc
      241 gggttcgcct gggcaacagc ggcgggcgcg ctcgccgcat tgcccggctt ggcgtccagg
      301 ggcctgccct ccagcttgtt gccgctgccg acaccgcgcg cggccgccgt gttgggatcg
      361 gctcccggcg gcagcacacg cacgacctgg cccacttcga tcaggttggg gttgtcgatg
      421 ttgttccact tgccgatgtc gcgccagttc tggccggtct ccaggccgat gcggatcagc
      481 gtgtcaccgg gcttgaccgt gtagtagccc ggcttgccgg cgttctcggc gccgggtggc
      541 ggcggcttga cctccgccga cggcagctcg gcgccggcgt ttgaggtcgg cgaggctgct
      601 cgcgcggcac tgttgctgcg atcctcgacc ggtgcccgat gcaaggaaga tgcgcaacca
      661 gccaagacca gcgcgacacc gatgccggcc aggcccagca agcgccgcgg catcaggaaa
      721 gacaacagtt cgatgaggta caggtgcatg tgtgcggttc ggtatctctc aaggaaacga
      781 aagaccaccc cggatcccta gcccgatcaa gggcggggcc agcttacttg ggggggaact
      841 caattaacgc cggattttaa ggggacgaaa tgcacggctt cgtgaaaaga gcgctgcaag
      901 ttgccagcca agtcgcggtc gacgacgacc agcacatggc cgccgcttgc ggcgtcgtgc
      961 atcggggcca caaggcgccc gcctggaccg agttgatcga gccaggcagc cggcagggcc
     1021 ccgcctccgg ctgccgcgat gatgccatcg taaggcgcat tgggagcgtg gccgagcatg
     1081 ccgtcgccgt agacgaggcg cagcccatcg ttgcgcaggc ccagcggcgg caactgcgcg
     1141 agatgctcgc gcgccttgtc gtgcaagggc cgcaggcgct ccacgctgat gacctggcgc
     1201 gccaggcgag ccagcacggc ggcctggtag ccgcagccgg tgccgacttc cagcacgcgg
     1261 ccgagcggcg ccgtgcggcc ggccagcatc agatcgatca tgcgtgcaac gaccgagggc
     1321 ttggaaatgg tctgcgacaa gccgatcggc aggctggtgt cctcgtaggc ttggttcgcc
     1381 agggccgtgt cgacgaaaag atggcgcggc accgccgcca tcgccgccag cacctgtacc
     1441 ccggccagtc cttgggcacg caagcgctcg accatgcgct ggcgtacgcc ctccgaatcc
     1501 aggcccagcc cggctggcgt agcgcggcgg gcgcgctcct gcacggcctc atgtaccggg
     1561 cgctgcggcc tcagcgtgct ttgcggcgta gcaggcgcga taggctgggc agccttgtgc
     1621 gtcgacgaag cgcgctgcgc cggcttggtc aggctgtcca gccgcagcgg aaacttcgat
     1681 gcgcggtcgg actccgcgtc accgctcatc cctgcacctc ggaggctgca ccttccagcc
     1741 aagcgcccca ggccggcaag gcgcggtgat ccgtcagatc cacttgcaaa ggcgtcaccg
     1801 agacgcaacc ctgagcggcg gcgtggaagt cggtgccctc gcccgcttcg cgcgcatcgc
     1861 cggccgggcc gatccagtag atggcctcgc cgcgcggatt ggtctgcctg atcaccggct
     1921 cgctcgcatg gcggcgcccc agacgggtaa tgcgatggcc cgccagctcg ctgtaaggtc
     1981 ggctcggaat attcacattg agcagccagg caccgcccgc cggcggcatc gcgatgacct
     2041 gctcgatcat cgagcgcgcc acgcgcgcag cagtgtccag atgctgccag cccttttcca
     2101 ccagcgaaaa ggcgatggcc ggaacgccga acagatagcc ttccatcgcg gccgccaccg
     2161 tgcctgagta cagcgtgtcg tcgcccatgt tggcgccgtt gttgatgccg gagaccacca
     2221 ggtcgggccg gtagccgagc agcccggtca aggccacgtg cacgcagtcc gaaggcgtgc
     2281 cgttgacata gcgaaagccg ttgcctgccg catagacgct cagcgggcga tgcagcgtca
     2341 gcgagttgga ggtgccgctg gcgttctgct cggg
//
LOCUS       DASUCY010000639         3198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_255475, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000639 DASUCY010000000
VERSION     DASUCY010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255475"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1389)
                     /gene="purH"
                     /locus_tag="VFV25_12640"
     CDS             complement(<1..1389)
                     /gene="purH"
                     /locus_tag="VFV25_12640"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEY1394214.1"
                     /translation="MTTALLSVSDKTGIVEFAQALHAAGVKLLSTGGTAKLLADQGLP
                     VTEVAEVTGFPEMLDGRVKTLHPRIHGGLLARRDVPAHMAALKEHGIATIDILVVNLY
                     PFAQATARPDCTLEDAIENIDIGGPAMLRAAAKNWQDVAVVIDPTDYERVLAELKAGS
                     IQRNTKFMLAKKVYAHTAAYDGMITNYLTALEAGAEGHTADVPKRGEYPAVFNLQLVK
                     AQDMRYGENPHQSAAFYRDAKPVAGSLAHYTQLQGKELSYNNIADADAAWECVKTFSA
                     PACVIIKHANPCGVAVGADAAEAYAKAFKTDPTSAFGGIIAVNRPLDKAAAELVAKQF
                     VEVLIAPSITAEARAVFAAKQNVRLLEVPLGDTPNALDFKRVGGGMLLQSADARNVAA
                     GELRIVTKKQPTPEQMQDLLFAWKVAKYVKSNAIVFCAGGMTLGVGAGQMSRIDSARI
                     ASIKAENAGLSLK"
     gene            complement(1466..1702)
                     /locus_tag="VFV25_12645"
     CDS             complement(1466..1702)
                     /locus_tag="VFV25_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HEY1394215.1"
                     /translation="MSKKHIEECLRDSLETYFKDLRGAEPHSMYDMILKTVEKPLLDV
                     VMQHADGNQSRAADWLGINRNTLRRKLVEHKLVK"
     gene            complement(1699..2742)
                     /gene="dusB"
                     /locus_tag="VFV25_12650"
     CDS             complement(1699..2742)
                     /gene="dusB"
                     /locus_tag="VFV25_12650"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEY1394216.1"
                     /translation="MRIGHIELPNRLFVAPMAGVTDRPFRRLCRRLGAGYAVSEMVTS
                     RKDLWASLKTSRRANHEGEAAPIAVQIAGTEPIMMAEAAAYNIERGAQIIDINMGCPA
                     KKVCNKWAGSALMQDEELALRIVEAVVTACEPQRVPVTLKMRTGWCRQHTNAVSIARG
                     AQDAGVAMITVHGRTREQGYKGEAEYETIAAVKRAVSVPVVANGDITSPEKAQAVLAA
                     TGADAVMIGRAAQGRPWIFREITHYLATGEHLPPPRVSEVKALLIEHLHDHYDLYGEF
                     TGVRSARKHIGWYVRALPRGELFRARMNTLDNSEQQLAAVVEYFDALAEQYERLPTTQ
                     ALEQDELLDQEAA"
     gene            2855..3198
                     /locus_tag="VFV25_12655"
                     /pseudo
     CDS             2855..3198
                     /locus_tag="VFV25_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543594.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
BASE COUNT          474 a         1032 c         1069 g          623 t
ORIGIN      
        1 cttcagcgac aggccggcgt tctccgcctt gatcgacgcg atgcgggcgc tgtcgatgcg
       61 gctcatctgg ccggcgccga cgcccagcgt catgccgccg gcgcagaaga cgatggcgtt
      121 gctcttgacg tatttcgcga ccttccaggc gaacagcagg tcctgcatct gttcgggcgt
      181 gggctgcttc ttggtgacga tgcgcaactc gcccgccgcc acgttcctgg cgtcggccga
      241 ttgcaggagc atgccgcccc ccactcgctt gaaatccaac gcgttcggcg tatccccgag
      301 cggcacctca agcagacgca cgttctgctt ggcagcgaac accgcacgcg cctcggccgt
      361 gatgctgggc gcgatcagca cctcgacgaa ctgcttggcc accagctcgg cggccgcctt
      421 gtccagcggc cggttgacgg cgatgatgcc gccgaaggcc gaggtcggat cagtcttgaa
      481 ggccttggca taggcctcgg ccgcgtcagc gcccacggcc acgccgcaag ggttggcatg
      541 cttgatgatc acgcaagccg gtgcgctgaa ggtcttcacg cattcccagg ccgcatcggc
      601 atcggcgatg ttgttgtacg agagctcctt gccctgcagc tgcgtgtaat gcgcaagact
      661 gccggccacc ggcttcgcgt cgcgatagaa agccgcgctt tggtgcgggt tctcgccgta
      721 gcgcatgtcc tgcgccttga cgagctgcag gttgaacacc gcgggatatt cgccgcgctt
      781 gggcacatcg gcggtatggc cctcggcgcc tgcttcgagt gcggtgaggt agttggtgat
      841 catgccgtcg taagcggcgg tgtgggcata gaccttcttg gccagcatga acttggtgtt
      901 gcgctggatg ctgccggcct tgagctcggc gagcacgcgc tcgtaatcgg tggggtcgat
      961 gacgaccgcc acgtcctgcc agttcttggc ggctgcgcgc agcatcgctg ggccgccgat
     1021 atcgatgttc tcgatcgcgt cttcgagcgt gcagtcgggc cgcgcggtgg cctgcgcgaa
     1081 cgggtagagg ttcaccacca ggatgtcgat ggtggctatg ccgtgctcct tgagcgcggc
     1141 catgtgcgcc ggcacatcac gccgcgccag caggccgccg tggatgcgcg ggtgcagcgt
     1201 cttgacgcgg ccgtccagca tctcaggaaa gccggtgacc tcggcgacct cggtgaccgg
     1261 caggccctgg tcggccagca gcttggcggt gccgccggtg gacagcagtt tcacgccggc
     1321 cgcgtgcagg gcctgggcga attcgacgat gccggtcttg tcggaaacgg aaaggagggc
     1381 ggtggtcatt tttcagcgat ccgtgaatgt gactggtgac gggttcgtaa tcagcgcctg
     1441 gtcaccagtc accgatcacc agtcactatt tgacaagttt gtgctcgacg agcttgcgtc
     1501 gcaaggtatt gcggttgatg cccagccagt cggcggcgcg cgactggttg ccgtcggcat
     1561 gctgcatcac gacgtcgagc aagggcttct cgaccgtctt gaggatcatg tcgtacatgg
     1621 aatggggctc ggcgcctcgc aggtccttga aataggtctc gaggctgtcg cgcaagcact
     1681 cttcgatatg tttcttgctc atgctgcttc ttgatcgaga agttcgtctt gttcaagcgc
     1741 ctgcgtcgtg ggcaagcgct catattgctc tgccagcgcg tcgaagtact ccaccaccgc
     1801 ggcgagttgc tgctcgctgt tgtccagcgt gttcatgcga gcgcggaaca actcgccgcg
     1861 cggcagagca cgcacatacc agccgatgtg cttgcgcgcg ctgcgcacgc cggtgaactc
     1921 gccgtacaag tcgtagtggt catgcagatg ctcgatcaac agggccttga cttcgctcac
     1981 gcgcggcggc ggcaggtgtt cgccggtggc caggtaatgc gtgatctcgc ggaagatcca
     2041 gggccggccc tgggcggcgc ggccgatcat gacggcatcg gccccggtgg cggcaagcac
     2101 ggcctgcgcc ttctcgggcg acgtgatgtc gccgttggcc accaccggca cgctcactgc
     2161 gcgtttcacg gcggcgatgg tctcgtactc ggcctcgccc ttgtagccct gctcgcgcgt
     2221 gcggccgtgc actgtgatca tcgccacgcc ggcatcctgc gcgccgcgcg cgatgctcac
     2281 ggcgttcgtg tgctgccggc accagccggt gcgcatcttg agcgtcaccg gcacgcgctg
     2341 cggttcgcag gccgtcacca cggcctcgac gatgcgcagc gccagctcct cgtcctgcat
     2401 caaggcagag ccggcccatt tgttgcacac cttcttggcc gggcagccca tgttgatgtc
     2461 gatgatctgc gcgccgcgct cgatgttgta ggcggcggcc tcggccatca tgatgggttc
     2521 ggtgccggcg atctgcaccg cgatgggcgc cgcctcgcct tcatgattgg cgcgccgcga
     2581 ggtcttgagg ctggcccaca gatccttgcg cgaggtcacc atctcgctca ccgcatagcc
     2641 cgcgcccagg cgcctgcaca gccggcgaaa cggccggtcc gtcacccctg ccatcggcgc
     2701 gacgaacagg cggttgggca gttcgatgtg gcctatgcgc atgggttggg gggagctgaa
     2761 ggaatttgcg actgctgaaa aaggaggcag agtatagcgt cgccgcgacc ccatcccacc
     2821 gctctgacgg gccgcgcggc aataatccgc agccatggat gcctggcttc aatcgctgct
     2881 gaacctgctc gccctgcccc acttcggcct gagcacggtc ttcgtgggtc gctgatttcg
     2941 gcgacgctgt tgccgctggg ttcggagccg gcggtgttcg ggctgatcaa gctcaacccc
     3001 gagttgttct ggccgacgat cctggtggcc accgccggca acacgctggg ggggtgcgat
     3061 cacctggtgg atgggctatg gcgccgagcg cgcctacgag cacatgacgc acaagcatcc
     3121 cgaaggccgc gccctgcaat ggctgcggcg cttcggcccc agggcctgtt actgtcctgg
     3181 ctgccggtgg tcggcgat
//
LOCUS       DASUCY010000640         4838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1299817, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000640 DASUCY010000000
VERSION     DASUCY010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4838
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1299817"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..874)
                     /locus_tag="VFV25_12660"
     CDS             complement(104..874)
                     /locus_tag="VFV25_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEY1394217.1"
                     /translation="MPTALIADDEPILREHLRSLLAKLWPELQIVAEARNGREAIELF
                     DTHAPQVLFLDIHMPGLNGIEAARSVARRGHVVFVTAYEQYAVQAFEAGAIDYVVKPF
                     DAERLEDTVQRLRGRLASGRDAGADDKLDAVIEQLSARLRSRQTGQAWLQWIKASIGQ
                     SLRLIPVEQVHYMRADEKYTLVVWDGGEALIRKSIRELADELDPERFVQIHRSTIVNL
                     HSVSQVTRGPNETAEVHLKDRSEVLPVSRSYVHLFRQM"
     gene            complement(884..1957)
                     /locus_tag="VFV25_12665"
     CDS             complement(884..1957)
                     /locus_tag="VFV25_12665"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEY1394218.1"
                     /translation="MSTQQATPPTTPPPMRWLAGLSAQRMAVALGLGVLGALLISPLF
                     ATSFPIVLGRMLVIALVLVLAYTLAGNWPQTLVPRWVAQVLAVGLAAPLTTFAIYLLF
                     VGGDFAQFLRHEGIQSGFLIISSISLFCGLVLALGALYRERDAQARAQALRFELERST
                     LERQALDAQLRALHAQIEPHFLFNTLANVQALVEAGSPRAAPVLRSLIAYLRAAMPRL
                     REQNSTLGAEVDLVHAYLEVMHLRMPDRLSYGIAIPAELQAQRFPSMALLTLVENAVR
                     HGVDPSEQGGRIDVGAERKGRELHVWVRDTGVGFSDKARPGAGLANLRERLSGFFGPG
                     AQLELSEALPHGVRAEIVFPADD"
     gene            complement(2117..>4838)
                     /locus_tag="VFV25_12670"
     CDS             complement(2117..>4838)
                     /locus_tag="VFV25_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEY1394219.1"
                     /translation="SAAATPETSAVQAGSAPAAAAPAASGAPAAASAPGAAASAPAAG
                     TPPAFANVIKGAKKIDGLFTLWQKDDKVWIELKPEDLGKPLLLSPKLATGIGENGIFG
                     GSMSLRFAAMEEQLVEFRRIHNQVQLIARNTEFFAKAGSPEGRAVAAAFSPSLLGSTA
                     VASQPHPTAKSILIEANALFVGDMLGLGQALQRSYRQSYAFDARNSAILRVRGKADSV
                     VFETQNHYATASIALPQPNTPPGTPVPTAPRTLPDARSLFLQLHYSLARLPETTMHPR
                     AADPRVGYFVTRVQDFSDDLARTPSVRYVNRWRLDKKDPSAELSEPVKPIIYWLDRTI
                     PEKYRGAITAGVLEWNKAFEKIGFKNAIVVKVQPEDADFDTLDVDVASIRWMTNASPQ
                     FGAIGPSHVDPRSGEILDADIAIESLSSRNLRAARSQILDARTAAAELPALMQLPDVA
                     APAAAGDAAAMHNPLQCDQAEMAAEQLGYMLDVLEARGELEPDSLEAQQFVLDYLKDV
                     TMHEVGHTLGLRHNFRASRLYTEAQLSDPEFTRTHALTGSVMEYAPINLARAGERGGT
                     PFQTTLGPYDYWAIEYAYKPIPADQEAAELKRIASHSAERQFAYGTDEDNFLGIDPEA
                     LQFDLGADPLAFARKRLDIARELLSRQEGRELKHGEDYSVLRRSVSYALRDVARATGV
                     AARQIGGVLTLRDFPGSGRDPLEPLPAATQRETLELLARGLLAADSFKISPALQRRLA
                     PDFQERTDAIFGGEPMVATDFSPAQQVLEVQRALLAQLMSDGVAVRILDSEAKAARGT
                     DAFRLSELYSRLDREVWSELAGGGDIPSLRRELQRDYVNRLSGLLLRPAALSRADARS
                     LLRAQAQSLLARINAAGRRGGLSAEARAHLQDSAETLQQALAAKLQRTGA"
BASE COUNT          746 a         1618 c         1730 g          744 t
ORIGIN      
        1 ggggcacaag gcggacaagg ccatggacgg cacgcgcatc gcgcacagcc atgcagccgc
       61 cgccgaggtc gtctccgaat aagttgttgc cgggctgcgg cggctacatc tgcctgaaca
      121 gatggacgta gctgcggctc acgggcagca cttcgctgcg atctttcaga tgcacttcgg
      181 ccgtctcgtt aggcccgcgc gtgacctggc tcacgctgtg caggttgacg atggtcgagc
      241 gatggatctg cacgaagcgc tccgggtcca gttcgtcggc cagttcgcgt atcgacttgc
      301 gtatcagggc ctcgccaccg tcccacacca ccagcgtgta cttctcatcg gcgcgcatgt
      361 agtgcacctg ctccaccggg atcagcctca ggctctggcc gatcgaggcc ttgatccatt
      421 gcagccaggc ctgccctgtc tgcctgctgc gcagccgcgc tgagagctgc tcgatcacgg
      481 catcgagctt gtcgtccgcg cccgcatcgc gccccgaggc caggcgcccg cggaggcgct
      541 gcacggtgtc ctccagccgc tcggcgtcga agggcttgac cacatagtcg atcgccccgg
      601 cctcgaaggc ctgcacggcg tattgctcat aagccgtcac gaacaccacg tggccgcgcc
      661 gcgccaccga gcgcgccgcc tcgatgccgt tgaggccggg catgtggatg tcgaggaaga
      721 gtacctgcgg cgcatgggtg tcgaagagct ctatcgcctc gcggccgttg cgcgcctcgg
      781 cgacgatctg cagctccggc cacagcttgg caagcaggct gcgcagatgc tcgcgcagga
      841 tgggttcgtc gtcggcgatc aatgcggtgg gcatgcgcgg gtcctagtca tcggccggga
      901 acacgatctc ggccctcacg ccatgcggca gcgcctcgct cagctccagc tgcgcgccgg
      961 ggccgaagaa accgctgagg cgctcgcgca aattcgccaa gcccgcaccg ggccgggcct
     1021 tgtcgctgaa gccgacgccc gtgtcgcgca cccacacatg cagctccctg cctttgcgct
     1081 cggcgccgac atcgatgcgc ccgccctgct cgctcgggtc gacaccgtgg cgcactgcat
     1141 tctcgaccag cgtcagcagg gccatcgacg ggaagcgctg cgcttgcaac tccgctggga
     1201 tcgcgatgcc atagctcaag cggtcgggca tgcgcagatg catcacttcg agataggcat
     1261 gcacgaggtc gacctcggca cccagtgtgg agttctgctc gcgcaggcgc ggcatcgcgg
     1321 cacgcagata ggcaatcagg ctgcgcagca ccggcgcggc gcgcggcgag ccggcctcga
     1381 ccagggcctg cacattggcc agggtgttga acaggaaatg aggctcgatc tgggcatgca
     1441 gcgcacgcag ctgtgcgtcc agagcctggc gctccagcgt gctgcgctcc agctcgaacc
     1501 gcagcgcctg ggcccgcgcc tgggcgtcgc gctcgcgata gagggcgccc agcgcgagca
     1561 ccagcccgca gaacaggctg atgctggaga tgatgaggaa gccagattgg atgccctcgt
     1621 ggcgcaggaa ctgagcgaag tcgccgccga cgaagagcag atagatcgcg aaggtggtga
     1681 gcggcgccgc gagtcccacg gccagcacct gcgccaccca gcgcggcacc aaggtctgcg
     1741 gccagttgcc ggccagggtg taggccagca ccagcaccag ggcgatgacc agcatgcgcc
     1801 ccagcacgat gggaaaggac gtcgcgaaga gcggactgat cagcagcgca ccgagcacgc
     1861 ccaggcccag cgcgaccgcc atgcgctggg cgctgaggcc ggccagccag cgcatgggcg
     1921 gcggggtggt aggaggggtg gcttgctgcg tggacatcgg ctcaacgata ccggcacggg
     1981 gtgcgctcta ctagccccgg cccgtccgga tgcgatgagc gacggaaagc agggcctagg
     2041 tggcggcccg catcagcaga gggagagccg gctgctgcct ggcaagcggc agccggcttc
     2101 atgggccgtt gcggcctcag gctccggtgc gctggagctt cgcagccagg gcctgctgca
     2161 gggtctcggc gctgtcctgc agatgggcgc gcgcctcggc gctcaggccg ccgcgccggc
     2221 cggcggcgtt gatgcgggcg agcaggctct gcgcctgggc gcgcagcagg ctgcgcgcat
     2281 cggcccggct cagcgccgca ggccgcagca gcaggccgga gaggcggttc acatagtcgc
     2341 gctgcagttc gcggcgcagc gaggggatat cgccgccgcc ggccagctcg ctccagacct
     2401 cgcgatccag ccggctataa agctcggaca ggcggaaggc atcggtaccg cgtgccgcct
     2461 tggcctcgct gtcaagaatg cgcactgcca cgccgtcgct catcagctgg gccagcaggg
     2521 cgcgctggac ttccagcacc tgctgggccg gcgagaagtc ggtggcgacc atgggttcgc
     2581 cgccaaagat ggcgtcggtg cgctcctgga aatcgggcgc caggcgccgc tgcagggccg
     2641 gcgagatctt gaagctgtcg gcggccagca ggccgcgcgc cagcagttcc agggtctcgc
     2701 gctgcgtagc ggcgggcaga ggctcaagcg ggtcgcgtcc gctgccgggg aagtcgcgca
     2761 gcgtcagcac gccgccgatc tggcgcgccg ccacgccggt ggcgcgcgcc acgtcgcgca
     2821 aggcatagct gaccgagcgg cgcagcactg aatagtcttc gccatgcttg agctctcggc
     2881 cctcctgacg cgacagcagc tcgcgcgcga tgtccagccg cttgcgcgcg aacgccagcg
     2941 ggtccgcgcc gaggtcgaac tgcagcgcct ctgggtcgat gccgaggaag ttgtcctcgt
     3001 cggtgccgta ggcgaactgg cgctcggcgc tgtgcgaggc gatgcgcttc agctcggcgg
     3061 cctcctggtc tgccgggatg ggcttgtagg cgtattcgat cgcccagtag tcataggggc
     3121 cgagcgtggt ctggaacggc gtgccgcccc gctcgccggc gcgcgccaga ttgatgggcg
     3181 cgtattccat caccgagccg gtgagcgcat gggtgcgcgt gaattccggg tcggacagct
     3241 gggcctcggt atacaggcgc gaggcgcgga agttgtgcct caggcccagc gtgtggccca
     3301 cctcgtgcat cgtgacgtcc ttcaggtagt cgagcacgaa ctgctgggcc tcgaggctgt
     3361 cgggctcgag ctcgccgcgc gcctcgagca cgtcgagcat gtagccgagc tgctcggccg
     3421 ccatctcggc ctggtcgcac tgcaaggggt tgtgcatcgc agccgcgtcg cctgccgcgg
     3481 ccggcgctgc aacatccggc agctgcatca gcgccggcag ctcggcggcc gcagtgcggg
     3541 catcgaggat ctgcgagcgc gccgcgcgca ggttgcgcga ggacaggctc tcgatggcta
     3601 tgtcggcatc gaggatctcg ccgctgcgcg gatcgacatg gctggggccg atggcgccga
     3661 actgcgggct ggcgttggtc atccagcgga tcgaggcgac atccacgtcc agcgtgtcga
     3721 agtcggcatc ttcaggctgc accttgacaa cgatggcgtt cttgaagccg atcttctcga
     3781 aggccttgtt ccactccagc acgccggccg tgatggcgcc gcggtatttc tcgggaatgg
     3841 tgcggtccag ccagtagatg atgggcttga ccggctcgga cagctcggcg ctcgggtcct
     3901 tcttatccag gcgccagcgg ttcacgtagc gcacgctggg cgtgcgcgcc aggtcgtcgc
     3961 tgaagtcctg cacacgcgtc acgaagtagc ccacacgcgg atcggcggca cgcggatgca
     4021 tagtcgtctc gggcagcctg gccagcgaat agtgcagctg caggaacagg ctgcgcgcat
     4081 cgggcagcgt gcgcggcgcg gtcggcaccg gcgtgccggg cggcgtgttg ggctggggca
     4141 gcgcgatgct ggcggtggcg tagtggttct gcgtctcgaa caccaccgag tcggccttgc
     4201 cgcgcacgcg caggatggcc gagttgcggg catcgaaagc atagctctgg cgatagctgc
     4261 gctgcagcgc ctggcccagg cccagcatat cgcccacgaa caaggcattg gcctcgatca
     4321 ggatggactt ggcggtcgga tgcggctggc ttgccaccgc cgtgctgccc agcaggctgg
     4381 gcgaaaaggc agccgcgacg gcgcggccct ccggcgagcc ggccttggca aagaactcgg
     4441 tgttgcgcgc gatcagctgc acctggttgt ggatgcggcg gaactcgacc agctgctcct
     4501 ccatggcggc gaagcgcagc gacatgctgc cgccgaagat gccgttctcg ccgatgccgg
     4561 tggccagctt cggcgacagc agcagcggct tgcccaggtc ttcgggcttg agctcgatcc
     4621 agaccttgtc gtccttctgc cagagcgtga acaggccgtc gatcttcttg gcgcccttga
     4681 tgacattggc gaaggcgggt ggcgtgccag cggcaggtgc cgaagccgcg gccccgggcg
     4741 ccgatgcggc ggcaggcgca ccagaggcag cgggcgccgc agcagccggc gcactgccgg
     4801 cctgcacggc cgacgtctcc ggcgtggcgg cggcagaa
//
LOCUS       DASUCY010000641         4524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1251456, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000641 DASUCY010000000
VERSION     DASUCY010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4524
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251456"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2068
                     /locus_tag="VFV25_12675"
     CDS             <1..2068
                     /locus_tag="VFV25_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HEY1394220.1"
                     /translation="MLMLCGCIGQSGGGWAHYVGQEKLRPQTGWTALAFALDWIRPPR
                     QQNSTSFFYAHTDQWRYEKLAVDEVLSPLADRKRHRGSMIDYNVRAERMGWLPSAPQL
                     RTNPLQVVRDAEKTRPGGDVKQYVVDALKSGALRMSCEDPDDPANWPRNMFVWRSNIL
                     GSSGKGHEYFLKHLLGTSHGVQGKDLGPQDTKPEEVAWHAQAPEGKLDLLVTLDFRMS
                     TTCLYSDIVLPTATWYEKNDLNTSDMHPFIHPLSTAVDPAWQARSDWEIYKGFAKKFS
                     EVCAGHLGVEKEVVLTPLMHDTPAELAQPFGVADWKRGEIDLVPGKTAPQIALVERDY
                     PNVYKRFTALGPLMGKVGNGGKGIAWNTQDEVRQLGELNGVVGESGATQGLPKIETDI
                     DAAEVVMMLAPETNGHVAVKAWEALSRQTGREHAHLALHREDEKIRFRDIQAQPRKII
                     SSPTWSGIESESVSYNAGWTNVHELIPWRTLTGRQQFYQDHPWMRDFGEGFTSYRPPV
                     DLKTIAQVQGKKPNGEKEVVLNFITPHQKWGIHSTYSDNLLMLTLNRGGPVVWISETD
                     ALAAGIEDNDWIELYNVNGAIAARAVVSQRVNPGMVLMYHAQEKIVNTPGSEITGQRG
                     GIHNSVTRIVLKPTHMIGGYAQLSYGFNYYGTIGTNRDEFVVVRKMRKIDWLDDEAAV
                     AEGVQA"
     gene            2065..3588
                     /gene="narH"
                     /locus_tag="VFV25_12680"
     CDS             2065..3588
                     /gene="narH"
                     /locus_tag="VFV25_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HEY1394221.1"
                     /translation="MRVRAQIGMVLNLDKCIGCHTCSVTCKNVWTSRPGMEYAWFNNV
                     ETKPGIGYPKEWENQDKWNGGWVRNADGSIVPRQGGKWKLLMKIFANPNLPQIDDYYE
                     PFTFDYDHLQSSPESKAAPTARPRSLVTGQRMEKIEWGPNWEEILGGEFSKRSKDKNF
                     EDIQKDIYGQFENTFMMYLPRLCEHCLNPACVASCPSGSIYKREEDGIVLIDQDKCRG
                     WRMCVSGCPYKKIYYNWNTGKAEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEAAASVEHDRDLYQAQLDVFLDPNDPAVIVQARADGIPEAWLDAARKSPVYKMAM
                     DWKIALPLHPEYRTLPMVWYVPPLSPITAAAQAGHMGVNGAIPDVNQLRIPVQYLANL
                     LTAGDTGPVVRSLERMLAMRAYQRERHVEGRENLAALQQVGLTQAEVQQMYQVMAIAN
                     YEDRFVIPSTHREYAENAFDMKGGCGFSFGNGCSEGSSETSLFGAKKRRTIPIKATL"
     gene            3612..4313
                     /gene="narJ"
                     /locus_tag="VFV25_12685"
     CDS             3612..4313
                     /gene="narJ"
                     /locus_tag="VFV25_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HEY1394222.1"
                     /translation="MTTNPGFAHRNACRALALLLCYPDDEMRALLPAIKAERPLEPAL
                     SGAACEALYRLADELLAADALEVQAQYVDCFDRGRRTSLHLFEHVHGDSRERGPAMID
                     LLQTYEKAGLYLTGGELPDYLPAVLEFASTQPADVAQAFLAEMAHILNALHAALQRKG
                     SPYAAVIAAVLELAGEPLRPIAVAEEEPVDDSWAEPEAFDGCSSAGSQRPAQPQPVHV
                     VRRAAHAAANPGAAA"
     gene            4310..>4524
                     /locus_tag="VFV25_12690"
     CDS             4310..>4524
                     /locus_tag="VFV25_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HEY1394223.1"
                     /translation="MNAFLFGIYPYVCLTVFLVGSLLRFDRDQYTWKSDSSQLLRAGQ
                     LRWGSNLFHFGILFLLFGHTVGLLTPH"
BASE COUNT          866 a         1520 c         1458 g          680 t
ORIGIN      
        1 catgctgatg ctgtgcggct gcatcgggca gtccggcggc ggttgggcgc actatgtggg
       61 gcaggaaaag ctgcgccccc agaccggctg gaccgcgctg gccttcgcgc tggactggat
      121 ccgaccgccg cgccaacaga actccaccag cttcttctat gcccacaccg accagtggcg
      181 ctacgagaag ctcgcggtcg acgaggtgct ttcgccgctg gccgaccgca agcgccaccg
      241 cggcagcatg atcgactaca acgtgcgcgc cgaacgcatg ggttggctgc ccagcgcgcc
      301 gcagctgcgg accaacccgc tgcaggtggt gcgcgacgcc gagaagaccc gcccaggcgg
      361 cgacgtgaag caatacgtgg tcgacgcgct gaagagcggc gcgctgcgga tgagctgcga
      421 ggacccggac gatccggcca actggccgcg caacatgttc gtgtggcgct ccaacatcct
      481 gggctcctct ggcaaggggc acgagtactt cctgaagcat ctgctgggca ccagtcatgg
      541 tgtgcaaggc aaggacctgg ggcctcagga cacgaagccc gaggaagtgg cctggcacgc
      601 ccaggccccc gagggcaagc tcgacctgct cgtgacgctc gacttccgca tgagcaccac
      661 ctgcctgtac tccgacatcg tgctgcccac ggcgacctgg tacgagaaga acgatctcaa
      721 caccagcgac atgcatccct tcatccaccc gctgtccacc gccgtggatc cggcttggca
      781 ggccaggagc gactgggaga tctacaaggg cttcgcgaag aagttcagcg aggtctgcgc
      841 cggccacctg ggcgtcgaga aggaagtggt gctcacgccg ctcatgcacg acacgccggc
      901 cgagctggcc cagcccttcg gcgtggccga ctggaagcgc ggcgagatcg acctcgtccc
      961 cggcaagacc gcgccgcaga tcgcgctggt cgagcgcgac tatcccaacg tctacaagcg
     1021 cttcacggcg ctcgggccgc tgatgggcaa ggtgggaaac ggcggcaagg gcatcgcctg
     1081 gaacacgcag gacgaggtgc ggcagctcgg cgagctcaac ggcgtggtcg gcgaatccgg
     1141 cgcgacccaa ggcttgccga agatcgagac cgacatcgat gccgccgagg tcgtgatgat
     1201 gctggcgccc gagaccaacg gtcacgtcgc ggtgaaggcc tgggaggcac tgtcgaggca
     1261 gaccggccgc gagcatgccc atctggcgct gcaccgcgag gacgagaaga tccgcttccg
     1321 cgacatccag gcgcagccgc gcaagatcat cagctcgccc acctggagcg gcatcgagag
     1381 cgagtcggtc tcgtacaacg ccggctggac caacgtgcac gagctcatcc cctggcgcac
     1441 gctcaccggg cgccagcagt tctaccagga ccacccctgg atgcgcgact tcggcgaagg
     1501 cttcacgtct tatcgcccgc cggtggacct gaagaccatc gcccaggtac agggcaagaa
     1561 gcccaacggc gagaaggagg tggtgctcaa cttcatcacg ccgcaccaga agtggggcat
     1621 ccattccacc tacagcgaca acctgctgat gctcacgctc aaccgcggcg ggccggtggt
     1681 gtggatcagc gagaccgacg cgctggcggc cggcatcgag gacaacgact ggatcgagct
     1741 ctacaacgtg aacggcgcca tcgccgcgcg agcggtggtg agccagcgcg tgaaccccgg
     1801 catggtcctc atgtaccacg cgcaggaaaa gatcgtgaac acgccggggt cggagatcac
     1861 cggccagcgc ggcggcatcc acaactcggt gacccgcatc gtgctcaagc ccactcacat
     1921 gatcggcggc tacgcgcaat tgagctacgg cttcaactac tacggaacca tcggcaccaa
     1981 tcgcgacgag ttcgtggtgg tgcgcaagat gaggaagatc gattggttgg atgacgaggc
     2041 tgctgtcgct gaaggagtgc aagcatgaga gtccgcgcgc agatcggcat ggtgcttaac
     2101 ctcgacaaat gcatcggttg tcacacctgt tcggtgacct gcaagaacgt gtggaccagc
     2161 cgacccggca tggagtacgc ctggttcaac aacgtcgaga ccaagccggg catcggctac
     2221 ccgaaggaat gggagaacca ggacaagtgg aatggcggct gggtgcgcaa cgccgacggc
     2281 tccatcgtgc cgcgccaggg cggcaagtgg aagctcctga tgaagatctt cgccaacccc
     2341 aacttgccgc agatcgacga ctactacgag cctttcacct tcgactacga ccacctgcag
     2401 tcgtcacccg aatccaaggc cgcaccgact gcgcgtccgc gcagcctcgt caccggccaa
     2461 cgcatggaga agatcgagtg gggcccgaac tgggaggaga tcctcggcgg cgagttcagc
     2521 aagcgctcga aggacaagaa cttcgaagac atacagaagg acatctacgg ccagttcgag
     2581 aacaccttca tgatgtacct gccgaggctg tgcgaacact gcctcaatcc ggcgtgcgtg
     2641 gcctcatgcc cctccggctc gatctacaag cgcgaggaag acggaatcgt cctcatcgac
     2701 caggacaagt gtcgcggctg gcgcatgtgc gtctcgggct gcccctacaa gaagatctac
     2761 tacaactgga acaccggcaa ggccgagaag tgcatcttct gctacccgcg catcgaggcc
     2821 ggccagccca cggtctgctc ggagacctgc gtgggccgca tccgctacct cggcgtgctg
     2881 ctgtacgacg ccgaccgcat cgaggcggcc gccagcgtcg agcatgaccg cgacctctac
     2941 caagcgcagc ttgacgtctt tctggacccg aacgacccag ccgtgatcgt gcaggcgcgt
     3001 gccgacggca tccccgaggc ctggctcgat gccgccagga agagcccggt ctacaagatg
     3061 gcgatggact ggaagatcgc gctgccgctg caccccgagt accgcacgct gccgatggtg
     3121 tggtacgtgc cgccgctgtc gcccatcacc gcggcggcgc aggctggcca catgggtgtg
     3181 aacggtgcca ttccagacgt caaccagctg cgcatcccgg tgcagtacct ggccaacctg
     3241 ctgaccgccg gcgacaccgg cccggtggtg cgctcgctgg agcgcatgct ggccatgcgc
     3301 gcctaccagc gcgagcgtca tgtcgagggc cgcgagaacc tggcggcgct gcagcaggtg
     3361 ggcctcacgc aggccgaggt gcagcagatg taccaggtga tggcgatcgc caactacgag
     3421 gaccgcttcg tcatccccag cactcaccgc gaatacgccg agaacgcctt cgacatgaag
     3481 ggcggctgcg gcttctcctt cggcaacggc tgctccgagg gttcgagtga aaccagcctg
     3541 ttcggcgcca agaagcgccg caccatcccg atcaaggcga cgctgtgagc gagaagaagg
     3601 agtcgaacac catgacgacg aatccaggct tcgcgcaccg caatgcctgc cgcgcgctgg
     3661 cgctgctgct gtgctatcca gacgacgaga tgcgcgcgct gctgcctgcg atcaaggccg
     3721 agcgcccgct cgaaccggcg ctttcgggcg ccgcctgtga agcgctgtac cggcttgccg
     3781 acgagctgct ggccgccgat gcgctggagg tgcaggcgca atacgtcgat tgcttcgacc
     3841 gcgggcgccg cacctcgctg cacctgttcg agcatgtgca cggtgactcg cgcgagcgcg
     3901 gcccagccat gatcgacctg ctgcaaactt atgagaaagc gggcttgtac ctgaccggcg
     3961 gcgaactgcc cgactacctg ccggccgtgc tggagttcgc ctccacgcag ccggccgatg
     4021 tagcgcaggc cttcctggcc gagatggcgc acatcctcaa tgcactgcat gcggcgctgc
     4081 agcgcaaggg cagcccgtac gctgcggtaa tcgccgcggt tttggagctg gccggcgagc
     4141 cgctgcggcc gatagcggtg gccgaagaag agccggtcga tgacagttgg gccgagccgg
     4201 aggccttcga cggctgcagc agcgccggca gccagagacc cgcccagcct cagcccgtgc
     4261 acgttgtgcg ccgcgccgcg cacgccgccg cgaacccggg agccgccgca tgaacgcctt
     4321 cctcttcggc atctacccct acgtctgcct caccgtcttt ctggtgggca gcctgctgcg
     4381 cttcgaccgc gaccagtaca cctggaagag cgactcgtcc cagctgctgc gcgccggcca
     4441 gctgcgctgg ggcagcaacc tgtttcactt cggcatcctg ttcctgctgt tcggccacac
     4501 ggtcgggctg ctgacgccgc actt
//
LOCUS       DASUCY010000642         6867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_30876, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000642 DASUCY010000000
VERSION     DASUCY010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 6867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6867
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30876"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2259)
                     /locus_tag="VFV25_12695"
     CDS             complement(<1..2259)
                     /locus_tag="VFV25_12695"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEY1394224.1"
                     /translation="MKQLLRLAATVCIGIGVFAHAALAASRSSEPINRAWTFTLGDVP
                     DGQAVERDTKGWQHVDVPHSFSQPYFLGTGFYVGHGWYRKTLELPASSAGKRISLEFD
                     GVFQDAEIFVNGQKAARHVGGYTGFTVDITSLVKPGRNLLAVHVNNEWNARVAPRAGE
                     HVFSGGIYRNVRLVVTDPVHVAWYGTFVTTPKVSEQQASVRVQTEVRNDRTGEARVAL
                     VSEVLDATGKRVSMQRSERTVPAAGTLNYDQSLPDIAQPRLWSPDSPTMYKLVSSLYV
                     DGKRVDRFETPFGIRAVRFTADQGFFLNGKHLYLIGANVHQDQAGWGDGVTDGAARRD
                     VKMVKDAGFNFIRGSHYPHSPAFTKATDELGMLFWSEAPFWGIGGFGADGNWLSSAYP
                     PDPADRPDFEASVLQQVEEMVRIHRNRPSIVIWSASNEPFFTTGEAMGAMRDFLKREV
                     DFFHRIDPTRPVAIGGAQRGEIDKLGDVAGYNGDGATLFLNPGVASVVSEYGSTMTDR
                     PGTYEPGFGLMPDTPDQKGSANPYSWRYRWRSGEALWAGFDHGSIASIEFGAMGFIDY
                     FRLPKRQYHWYRNTYRGIAPPMWPVEGKPARLQLAASQKLIRGTQGLDDAHVVVTVHD
                     EAGRALSNSPDVTLTIVSGPGQFPTGRSIRFGNQSLVAIRDGQAAITFRSYFAGKTVI
                     EATSPGLKPARITIATTGPDPFIPGRSPLAPDQPVIAYPPINRTALASDPINVTTNRP
                     TSTSGAEAGHTAP"
     gene            complement(2298..4727)
                     /locus_tag="VFV25_12700"
     CDS             complement(2298..4727)
                     /locus_tag="VFV25_12700"
                     /inference="COORDINATES: protein motif:HMM:NF025851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEY1394225.1"
                     /translation="MHGRRHFIASAGLAFAFPGLAHAKVLATEREGRKDLSLWYDLPA
                     GPWIEALPVGNGRLGAMVFGRPAQERLQFNIDTLYGGGPYVPDNPGFREALPKVRELI
                     DQERWKEANDLVNEALMGRPIKQMPFGAAGDLLLDFPGLAGAADYRRSLDLDSAVAAT
                     TFGNGKVRHRREVFSSVPDQVVVIRLEAQHGTIDLDLSYRHPDRPEYGAPRRDANGAV
                     FLGAAWDHREPLQADKRPPSLAIRADGRDALLVEGRNIDAFGIAGVLRYALRVRAVGD
                     GRIEAEGEHLRVRGARQLMLVVAGETSYVNFQDTSGDPVAAVRARTDAAARKPWSKLR
                     ADHLQAHRALFRRLHIDLGGHEAHDRPTHQRIAAVPRQPDAALAALYVQYARYLLLSS
                     SRPGSQPANLQGLWNEGVHPPWEGKYTININTQMNYWPAGPANLGECVEPLLKMVEEL
                     AVNGARTARNSYGARGWVAHHNTDLWRAAAPIDGALWGMWPTGGAWLCTTLWGHYQYH
                     PSPALLHRLVPLFSGASLFFLDTLVEDPKGRGLVTSPSLSPENEHHPKVALCAGPAMD
                     SQILRDLFDATLEAQALLGERDPAFTEALRKARARLPADRIGAQGQLQEWLEDWDAVA
                     PEQQHRHVSHLYAVYPSGQINVRDTPKLAQAARVTLNVRGDESTGWATAWRLALWARL
                     GDGERAYRILQGLLGPRRTYPNMFDAHPPFQIDGNFGGAAGILEMIVQSWGGEVRLLP
                     ALPAAWPRGEVRGVRVRGGIELDLAWSRGRPTRLQLRGRPKTEVKLRHGERLLTLKLD
                     AAGRASLTEFG"
     gene            complement(4745..6046)
                     /locus_tag="VFV25_12705"
     CDS             complement(4745..6046)
                     /locus_tag="VFV25_12705"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEY1394226.1"
                     /translation="MTCAPLRSLGSLPLLGALLFHPLQPASGAEVTAPTPLPTVAAQA
                     AGFSPQRLDALHRLMGREVDAGRYSGYIALLARGGKIVDWRAHGWQNIDSRTPLRTDS
                     IVRIFSMSKIVTTVSILVLLEEGRLKLDDPVERYLPALKDRQVVVGGTPDAPLLEPAK
                     RSVTIRDLLTHTSGYSYGDPGSDDAVPAALMQRAKMWEAANLDEFVARLAPVPLNEQP
                     GTRFRYGVSTDILGAVVEKASGQRLDTFLRERIFSPLRMVDTGFWVPASKLARVATIY
                     ERDPQGRLVPTGWANIAGAGPDHGILSGGGGLYSTAADYLRFAQMLLNGGQLDGVRIL
                     GRKTVALMTSNHLKHLADPHPSGMRSQGFGLGVRVLVDPGEGSTLGSPGAFGWEGAAT
                     TFVQIDPKERTVALLLLQHMPFNQDDIISTFTNGYYAALVD"
     gene            complement(6043..>6867)
                     /locus_tag="VFV25_12710"
     CDS             complement(6043..>6867)
                     /locus_tag="VFV25_12710"
                     /inference="COORDINATES: protein motif:HMM:NF022039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 catalytic
                     domain-containing protein"
                     /protein_id="HEY1394227.1"
                     /translation="LLWAHWKHVDARMDEVLDTYARWGIKGVKVDFMNRDDQDVVDFF
                     HRIAAATAKRRLLLDLHGAYVPAGLQRSFPNFITQEGVLGAEWSKMDRRVTPSHNLML
                     PYTRMLAGPMDYTPGGFRNATPATFEVRAEMPLVQNTRGQTLAMYVVYDSPLQMVSDD
                     PAAYRGEPGFEFIKRVPTAWDETRFIGGEPGRDIVLARRNGKAWYIGAMTSDEARPER
                     VPLRFLPSGRWRATIWEDGDTTRALRRSERIVTRADALVLKLAVAGGSAVILEPMP"
BASE COUNT          995 a         2295 c         2487 g         1090 t
ORIGIN      
        1 cggcgccgtg tggccggcct cggcgccgct ggtcgacgtc ggccggttgg tggtgacgtt
       61 gatcggatcg ctggcgagag cggtacggtt gatcggcggg taggcgatga cgggctggtc
      121 gggcgccagc ggcgaccggc cgggaatgaa cgggtcgggc ccggtcgtcg cgatcgtgat
      181 gcgcgccggc ttcaggccgg gcgacgtggc ctcgatgacg gtcttgccgg caaagtacga
      241 gcggaacgtg atcgccgcct ggccgtcgcg gatggcgacc agggactggt tgccgaagcg
      301 gatgctgcgg ccggtcggga actggcccgg accgctgacg atggtgagcg tcacgtcggg
      361 cgagttgctc agcgcgcgcc ctgcttcgtc gtgcacggtc acgaccacat gggcgtcatc
      421 cagcccctgg gtgccgcgga tcagtttctg gctggccgcc agctgcagcc gcgccggctt
      481 gccttcgacc ggccacatgg gcggtgcgat gccgcggtag gtgttgcggt accagtggta
      541 ctggcgcttg ggcagccgga agtagtcgat gaagcccata gcgccgaact cgatggacgc
      601 gatcgagcca tgatcgaagc cggcccacag ggcctcgccg gagcgccagc ggtagcgcca
      661 tgaatagggg ttggcgctgc ccttctggtc cggcgtgtcg ggcatcagcc cgaagccggg
      721 ctcgtaggtg ccgggccggt cggtcatcgt ggagccgtat tccgacacca ccgaggcgac
      781 gcccgggttc aggaacagcg tcgcgccgtc gccgttgtag ccggccacgt cgcccagctt
      841 gtcgatctcg ccgcgctggg cgccgccgat ggccaccggc cgtgtcgggt cgatgcggtg
      901 gaagaagtcg acctcgcgct tcaggaagtc gcgcatggca cccatcgcct cacccgtcgt
      961 gaagaagggc tcgttggagg cgctccagat gacgatggac ggacggttgc ggtggatgcg
     1021 caccatctcc tcgacttgct gcagcacgct ggcctcgaag tcggggcggt ccgccgggtc
     1081 gggcgggtag gcgctggaca gccagttgcc atcggcgccg aagccgccga tgccccagaa
     1141 cggcgcctcc gaccagaaca gcatgccgag ctcgtcggtg gccttcgtga aggccggcga
     1201 atgcgggtag tgcgagccgc ggatgaagtt gaagccggcg tccttgacca tcttcacgtc
     1261 gcgccgcgcg gcgccgtcgg tcacgccgtc gccccagccg gcctgatcct ggtgtacgtt
     1321 agcgccgatc aggtagaggt gcttgccgtt gaggaagaag ccctggtcgg cagtgaagcg
     1381 caccgcgcgg atgccgaaag gcgtttcgaa gcggtcgacg cgcttgccgt cgacgtacag
     1441 gctgctgacg agcttgtaca tcgtgggact gtccggcgac cacagccgcg gctgcgcgat
     1501 gtcgggcagc gactggtcgt agttgagcgt gcctgcggcg ggtaccgtgc gctcactgcg
     1561 ctgcatcgac acccgcttgc cggtcgcgtc gagcacctcg gagacgagag ccactcgtgc
     1621 ttcccctgtg cggtcattgc ggacctcggt ctgcacgcgc accgaggcct gctgctcaga
     1681 gaccttgggc gtcgtcacga aggtgccgta ccaggccaca tgcacggggt cggtgacgac
     1741 caggcgcacg ttgcggtaga tgccgcccga gaacacatgc tcgccagcgc gcggcgccac
     1801 gcgggcgttc cactcgttgt tgacgtgcac ggccagcagg ttgcggcccg gcttcaccaa
     1861 cgatgtgatg tcgacagtga agcccgtgta gccgccgacg tggcgggcgg ccttctggcc
     1921 gttgacgaag atctccgcgt cctggaagac gccgtcgaac tcgagcgaga tgcgcttgcc
     1981 ggccgaggag gccggcagct ccagcgtctt gcgataccag ccatgaccga catagaagcc
     2041 ggtgccgagg aaatagggct gcgagaacga atgcggcacg tccacatgct gccagccctt
     2101 ggtgtcacgc tcgacggcct gcccgtcggg tacatcgccg agcgtgaacg tccaggcgcg
     2161 gttgatgggc tcgctgctgc gggacgccgc caaggccgca tgggcgaaca cgccgatacc
     2221 gatgcacacg gtcgccgcaa ggcggagaag ttgcttcatc gcgatgtcat tgttctgtcg
     2281 ggcttcaatc aaggcggtca gccgaactcg gtgaggctgg cgcggcccgc ggcgtcgagc
     2341 ttcagcgtga gcagcctctc gccgtggcgc agcttcacct cggttttcgg ccggccgcgc
     2401 agttgcagcc gcgtcgggcg cccccggctc catgcaaggt cgagttcgat gccgccgcgc
     2461 acgcgcaccc cacgcacctc gccgcgcggc caggccgcgg gcagcgccgg cagcaggcga
     2521 acctcgccgc cccacgactg cacgatcatc tccaggatgc cggccgcgcc gccgaagttg
     2581 ccgtcgatct ggaagggcgg gtgcgcgtcg aacatgttgg ggtaggtgcg ccgtggcccc
     2641 agcagcccct gcaggatgcg gtaggcgcgc tcgccgtcgc caagccgggc ccacagcgcc
     2701 aggcgccagg cggtggccca gccggtgctc tcgtcgccgc gcacattgag cgtgacgcgt
     2761 gcagcctgcg ccagcttggg ggtgtcgcgc acgttgatct ggccgctggg gtagacggcg
     2821 tacaggtgcg agacgtggcg gtgctgctgc tcgggtgcga cggcgtccca gtcttccagc
     2881 cactcctgca gctggccctg tgcgccgatg cggtcggccg gcaggcgcgc acgcgccttg
     2941 cgcagcgcct cggtgaacgc ggggtcgcgc tcacccagta gcgcctgcgc ttcgagcgtc
     3001 gcgtcgaaca ggtcgcgcag gatctggctg tccatcgccg gcccggcgca cagcgcgacc
     3061 ttcgggtggt gctcgttctc gggcgagagc gacggcgagg tgaccaggcc ccggcctttc
     3121 gggtcttcga ccagcgtgtc gaggaagaac agcgacgcgc cgctgaacag cggcaccagg
     3181 cggtgcagca gcgcggggct ggggtggtac tggtagtggc cccagagcgt ggtgcacagc
     3241 caggcgccac cggtgggcca catgccccac agcgccccgt cgatcggcgc cgcggcgcgc
     3301 cagaggtcgg tgttgtggtg cgccacccag ccccgcgcgc cgtagctgtt gcgcgcggtg
     3361 cgggcgccgt tgacggccag ctcctccacc atcttcagca gcggctcgac gcactcgccc
     3421 aggttggccg gcccggcggg ccagtagttc atctgggtgt tgatgttgat cgtgtacttg
     3481 ccctcccagg gcgggtgcac gccttcgttc cacaggccct gcaggttcgc cggttggctg
     3541 cccggccgcg acgacgacag cagcaggtag cgtgcgtact gcacgtacag cgcggcgagc
     3601 gccgcatcgg gctgccgggg cacggcggcg atgcgctggt gggtcggccg gtcgtgggcc
     3661 tcgtggccac ccaggtcgat gtgcaggcgg cggaacagcg cgcggtgcgc ctgcagatgg
     3721 tcggcgcgca gcttcgacca gggtttgcgc gcggcggcgt cggtgcgcgc gcgtaccgcg
     3781 gccaccgggt cgccgctggt gtcctggaag ttcacgtagc tggtctcgcc ggcgacgacc
     3841 agcatcagct ggcgggcccc gcgcacgcgc aggtgctcgc cttcggcctc gatgcggccg
     3901 tcgcccaccg cgcgcacgcg cagcgcataa cgcagcacgc cggcgatgcc gaaggcgtcg
     3961 atgttgcgcc cttcgaccag cagcgcgtcg cggccgtcgg cgcggatggc gagcgacggc
     4021 gggcgcttgt cggcctgcag cggctcgcgg tggtcccacg cggcgccgag gaacaccgcg
     4081 ccgttggcgt cgcgcctcgg cgcgccgtac tcggggcggt cggggtggcg gtacgacagg
     4141 tcgaggtcga tcgtgccgtg ctgggcctcc aggcggatca ccaccacctg gtcgggcacg
     4201 ctgctgaaca cctcgcgccg gtggcgcact ttgccattgc cgaaggtggt ggcggcgacc
     4261 gcgctgtcga ggtcgaggct gcggcggtag tccgcggcgc ccgcgaggcc gggaaagtcc
     4321 agcagcaggt caccggcggc gccgaaaggc atctgcttga tcggccggcc catcagggcc
     4381 tcgttgacga gatcgttcgc ttccttccag cgttcctggt cgatcagctc gcgcaccttg
     4441 ggcaacgctt cccggaaccc ggggttgtcc ggcacgtagg gcccgccgcc gtacagcgtg
     4501 tcgatgttga actgcaggcg ctcctgcgcg gggcggccga acaccatggc ccccaggcgg
     4561 ccgttaccga ccggcagcgc ctcgatccag ggcccggcgg gcaggtcgta ccacagcgac
     4621 aggtccttgc ggccttcgcg ctcggtggcc agcaccttgg cgtgtgccag accggggaag
     4681 gcgaaggcca gcccagccga tgcgatgaag tgacgtctac cgtgcacgtt ctgtctccgg
     4741 gtgttcaatc gacgagcgcg gcgtagtagc cgttcgtgaa cgtcgagatg atgtcgtcct
     4801 ggttgaacgg catgtgctga agcaggagca gtgcgaccgt gcgttccttc gggtcgatct
     4861 gcacgaaggt cgtggccgcc ccttcccagc cgaatgcgcc gggcgagccg agcgtggagc
     4921 cttctccggg atcgaccagc acgcgcacgc cgaggccgaa gccctgactt cgcatcccgg
     4981 acggatgcgg gtccgcgagg tgcttcaggt ggttcgacgt catcagtgcc accgtcttgc
     5041 ggccaagaat tcgaacacca tcgagctgcc cgccgttgag cagcatctgt gcgaaacgca
     5101 gatagtcggc cgccgtcgag tagagcccgc cgccaccgct caggatgccg tggtcagggc
     5161 cggctccagc gatattggcc catccagtcg ggaccagcct gccctgcggg tcgcgttcgt
     5221 agatggtcgc cacgcgcgcg agcttgcttg ctggcaccca gaaacccgtg tcgaccatgc
     5281 gcagcgggct gaagatgcgc tcgcgaagga aggtgtcgag acgctgcccc gaggccttct
     5341 cgacgaccgc gccgaggatg tcggtgctga cgccatagcg aaagcgcgtg ccgggctgct
     5401 cgttcagcgg caccggcgcg agccgcgcaa cgaactcgtc gagattggca gcctcccaca
     5461 tcttggcgcg ctgcatcagc gccgcgggca cggcgtcgtc agacccgggg tcgccgtagg
     5521 aatagcccga ggtgtgggtg agcagatccc ggatcgtgac cgatcgcttt gcgggctcga
     5581 gcagcggagc atcgggcgtc cccccgacaa cgacctggcg atccttcagc gctggcagat
     5641 acctttccac cggatcgtcc agcttcaggc gcccttcctc gagcaggacc aggatggaca
     5701 cggtggtgac gatcttcgac atcgagaaga tgcggacgat cgagtccgtc ctcagcggcg
     5761 tcctgctgtc gatgttctgc cagccgtggg cgcgccagtc cacgatcttc ccgccacgag
     5821 ccagcaaggc gatgtagccg gagtacctgc ccgcgtcgac ttcgcggccc atcaggcggt
     5881 gaagtgcatc gagtcgctgc ggtgaaaatc ccgccgcctg cgctgcgacc gtcggcaacg
     5941 gcgtcggggc cgtgacctcg gcgccgcttg ccggctgcaa gggatggaac agcaaggcgc
     6001 caaggagggg cagcgatccc agggagcgaa gtggggcgca ggtcatggca tcggttcgag
     6061 gatcacggcg ctaccgccgg ccacggccag cttcagcacc agtgcgtcgg cgcgcgtcac
     6121 gatgcgttcg ctgcgccgca gcgcgcgcgt cgtgtcgccg tcctcccaga tcgtggcgcg
     6181 ccagcgcccg gagggcagga agcgcagcgg cacgcgctcg gggcgtgcct cgtcggaggt
     6241 catcgcgccg atgtaccagg ctttgccgtt gcgccgcgcg agcacgatgt cgcgccccgg
     6301 ctcgccgccg atgaagcgcg tctcgtccca ggccgtcggc acgcgcttga tgaactcgaa
     6361 gccgggctcg ccgcggtagg ccgcggggtc gtcggacacc atctgcagcg ggctgtcgta
     6421 gaccacgtac atggccagcg tctggccgcg cgtgttctgc accagcggca tctcggcgcg
     6481 cacctcgaag gtggccggcg tcgcgttgcg gaagcccccg ggcgtgtagt ccatcgggcc
     6541 ggccagcatg cgcgtgtagg gcagcatcag gttgtggctg ggcgtgacgc gcctgtccat
     6601 cttgctccac tcggcgccca gcacgccttc ctgggtgatg aagttgggga agctgcgctg
     6661 caggccggcc ggcacatagg cgccgtgcag gtccagcagc agcctgcgct tggcggtggc
     6721 ggcggcgatg cggtggaaga agtccaccac gtcctggtcg tcgcgattca tgaagtcgac
     6781 cttgacgccc ttgatgcccc agcgggcata ggtgtccagc acctcgtcca tgcgtgcatc
     6841 gacgtgcttc cagtgcgccc acagcag
//
LOCUS       DASUCY010000643         5385 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_962240, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000643 DASUCY010000000
VERSION     DASUCY010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 5385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5385
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962240"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VFV25_12715"
     CDS             <1..211
                     /locus_tag="VFV25_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="band-7 C-terminal domain-containing protein"
                     /protein_id="HEY1394228.1"
                     /translation="IRKIAASIQQPGGEQAVQLKVAEKAVDAYAQLAQKNNTMIVPGN
                     MGEVSGLIGTAMALMKRLQPAAAAK"
     gene            complement(306..845)
                     /gene="def"
                     /locus_tag="VFV25_12720"
     CDS             complement(306..845)
                     /gene="def"
                     /locus_tag="VFV25_12720"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEY1394229.1"
                     /translation="MAVKEILKMGDWRLLRVAQPVTEFDTPELHILIDDMFDTMAAAD
                     GAGLAAPQIGVDLQVVIFGASKRNPRYPDAPIVPRTVLLNPVITPLGEEMEEGWEGCL
                     SVPGLRGLVPRHARIRYSGYDPKGKRIEREVEGFHARVVQHECDHLIGKLYPMRVRDF
                     TQFGFTEVLFPELDPPMDE"
     gene            994..1608
                     /locus_tag="VFV25_12725"
     CDS             994..1608
                     /locus_tag="VFV25_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEY1394230.1"
                     /translation="MQAAATQHESKTRFLDAALRVIRTKGYEATTVDDLCAAAGLTKG
                     SFFHHFDGKEALALEAARHFGAHAQAVFASAPYQSLADPLARLLGYVDFRRALLKGEL
                     PEFTCLLGTMVQETYETHPAIRAACDEQLKEHVVTLAGLVAEAKKRYTPRAKWSAQSI
                     ANFTQATIQGAFVLAKAQHDAAVADECLLHLRRYLALLFTKPKE"
     gene            1614..2390
                     /locus_tag="VFV25_12730"
     CDS             1614..2390
                     /locus_tag="VFV25_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY1394231.1"
                     /translation="MATKIPGFFWYDVTTRDPKAAAAFYSSVMGWAAQDSGVPGQTYT
                     IFSKGPNPIGGLMSFPEGSPPTGVQSCWTGYIGVDDVDAYTKRVKAAGGAIHREPWEI
                     PGVGRFAIVADPQGAVFMLMRGFSDQPPPQVAANTPGHVAWHELHADDPAKAFAFYAD
                     LFGWTKGEAMDMGPGGIYQLFKFGGGEPVGGMMTRTPDAPGSFWLYYFQVEALGAALA
                     RVTAGGGKICSEPHQVPGDAWIAPCLDPQGGAFALVADKR"
     gene            complement(2450..2526)
                     /locus_tag="VFV25_12735"
     tRNA            complement(2450..2526)
                     /locus_tag="VFV25_12735"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2490..2492),aa:Val,seq:gac)
     gene            complement(2560..4827)
                     /locus_tag="VFV25_12740"
     CDS             complement(2560..4827)
                     /locus_tag="VFV25_12740"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013803154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEY1394232.1"
                     /translation="MKTGLQETAAQAAPIVQLAGEAQAEQAPAAEEALLAKAAAFSQP
                     LLAGQRLDTGEDALAHADGVAAILRGIGAAPSMRAAAYLVYAADFLNKPEEMVAKAFG
                     ESYASLVAHTRKLVQVQRSARDALADEAAERDGAVQAEQLERVRKMLLAFSRDLRVVL
                     MRLASRLQTLRYYAASKRPCPGVLARESLQVFAPLANRLGIWQIKWELEDLSFRFLQP
                     ERYMAIARQLDEKRVEREQVVEAARARLEVELRRAGVQAQVQGRPKHLYSIWKKMQGK
                     GLTLERVFDVRALRVIVPDVRACYAALSRVHELWRSLPEEFDDYIAKPKPNGYQSLHT
                     VVLDEEGRAIEIQIRTREMHEHAESGVAAHWAYKEAGARGYRGVSAAGDFEEQVAQAR
                     KAVLRQLLAWEHDLATAGAPEPRAGEQGLFDDRIYVFTPQGAIVELPAGATPVDFAYT
                     VHTDLGHRCRGAKVDGAMVPLNTPLASGQTVEVIALKEGGPSLDWLNPELGYLQSTRA
                     RAKVRAWFNALAQQQTIARGREAVEKLLQREGRTAVKLEELAAQLGFRNADALFEVIG
                     KDEYSLKNIEHLFKPAEPLPSPDEALAQRLSRGGGEAAAPKGGVLVVGVESLLTTLAR
                     CCKPAPPDAISGYVTRGKGVAIHRSDCSNFRQMSGRNPERVIAVAWGGARTDKPALYP
                     VDVAVEANDRQGLLRDISEVFAKEKMNVIGVNTVTVKGMAWMTFTVEVTDSARLSSVL
                     GIVRQVNGVRNARRK"
     gene            complement(4824..>5385)
                     /locus_tag="VFV25_12745"
     CDS             complement(4824..>5385)
                     /locus_tag="VFV25_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEY1394233.1"
                     /translation="WVGTSMGGLIGIGMASLPDTPIRRLVLNDVGPTIEPNSIKRIGD
                     YLGKPLRYASVDEAADYLWSISKGFGPHTREQWLALTQPQIKPDGDGWVSRYDPRIAL
                     AFNGVTPELAAAGEAALWQRYDAISCPTLLIRGEQSDLLSRDTAQAMTRRGPKAKLVE
                     LPGIGHAPTLLAADQVALVKEFLTGP"
BASE COUNT          888 a         1866 c         1748 g          883 t
ORIGIN      
        1 gatccgcaag atcgccgcct ccatccagca gccgggcggc gagcaggccg tgcagctcaa
       61 ggtggccgag aaggcggtgg acgcctatgc ccaactcgcg cagaagaaca acacgatgat
      121 cgtgccgggc aacatgggcg aggtctcggg cctgatcggc acggcgatgg cgctgatgaa
      181 gcgactgcag ccagcggcgg ccgccaagta aggcctaggc cttacgctat cttttttgta
      241 ttactagccc gcaaatgtcg ctgggcagct ggcagaaaag ttgcaaaaac ttatcgaggc
      301 cgggctcact cgtccatggg cggatcgagc tcggggaaca gcacctcggt gaagccgaac
      361 tgcgtaaagt cgcgcacccg catcggatag agcttgccga tgaggtgatc gcattcgtgc
      421 tgcacgacgc gagcgtggaa gccctccacc tcgcgctcga tgcgcttgcc cttggggtcg
      481 tagccgctgt agcggatgcg cgcgtggcgc ggcaccaggc cgcgcagacc gggcaccgag
      541 aggcagcctt cccagccctc ctccatctcc tcgcccagcg gcgtgatcac cgggttgagc
      601 agcaccgtgc gcggcacgat gggcgcgtcg gggtagcgcg ggttgcgctt gctggcgccg
      661 aagatcacca cctgcagatc gacgccgatc tgcggcgcag ccaggccggc gccgtctgcg
      721 gcggccatgg tgtcgaacat gtcgtcgatc aggatgtgca gctcgggcgt atcgaactcg
      781 gtcaccggct gggccacgcg cagcaggcgc caatcgccca tctttaggat ctctttgacg
      841 gccatggtgc gtttcgtctt cgcgctggag tgatgcgatg gcgtgatttt cgccgctgcg
      901 gcgctcgccg gcaggcgatc ccaggacgcg ccgcgtggac ttgacacgga tagtttccgc
      961 gcttaaaaca taccaactgg ttggtataag catatgcaag cggcagcgac tcagcacgaa
     1021 tccaagaccc gcttcctcga cgcagccctg cgcgtgatcc gcaccaaggg ttacgaggcg
     1081 acgacggtgg acgacctctg cgcagccgcc gggctcacca agggcagctt cttccaccac
     1141 ttcgatggca aggaggcgct ggccctcgag gcggcgcggc atttcggtgc gcatgcccag
     1201 gccgtattcg ccagcgcgcc ctatcagtcg ctggccgacc cgctggcacg cctgctcggc
     1261 tatgtcgatt tccgccgcgc gctgctgaaa ggcgaactcc ccgagttcac ctgtttgctc
     1321 ggcaccatgg tgcaggagac ctacgagact cacccagcga tccgcgcagc ctgcgacgag
     1381 cagctcaagg aacatgtggt gacgctggcc ggcctcgtcg ctgaggccaa gaagcgctat
     1441 acgccgcgcg cgaaatggag cgcccaaagc atcgccaact tcacccaggc cacgatacaa
     1501 ggtgccttcg tgctcgccaa ggcccagcac gacgccgccg tcgccgacga atgcctgctt
     1561 cacttgcggc gctatctggc gcttttattc accaaaccga aggagtagac agcatggcca
     1621 ccaagatccc tggtttcttc tggtacgacg tcaccacccg cgaccccaag gccgccgcgg
     1681 ccttctacag cagcgtgatg ggctgggccg cccaggactc gggcgtgccc ggacagacct
     1741 atacgatctt ctccaaaggg ccgaacccca taggcggtct catgtccttc cctgaaggtt
     1801 cgcccccgac aggcgtgcag tcctgctgga ccggctacat cggcgtcgac gatgtcgatg
     1861 cctataccaa gcgcgtcaag gccgccggcg gtgcgatcca ccgcgaaccg tgggagatcc
     1921 ccggcgtggg gcgcttcgca atcgtggccg acccgcaagg cgcggtcttc atgctgatgc
     1981 gcggcttcag cgaccagccg cctccgcaag tcgcagccaa cacgcccggc cacgtcgcct
     2041 ggcatgagct gcacgccgac gacccggcaa aggcgtttgc cttctacgcc gacctcttcg
     2101 gctggaccaa gggcgaagcg atggacatgg ggcccggcgg catctatcag ttgttcaagt
     2161 tcggcggcgg cgaaccggtg ggcggcatga tgaccaggac gcccgatgcg ccaggatcgt
     2221 tctggctgta ctacttccaa gtcgaagctc tgggcgccgc gctggcacgc gtcaccgccg
     2281 gcggcggcaa gatctgcagc gagccgcatc aggtgccggg tgacgcgtgg atcgctcctt
     2341 gcctcgatcc tcagggcggc gccttcgcac tggttgccga caagcgctga cggcaagccc
     2401 caatcaaagc aaaaagcctc gactccgcaa ggaatcgagg cttcggtatt ggtaggcgcg
     2461 attggactcg aaccaacgac ccccaccatg tcaaggtggt gctctaacca gctgagctac
     2521 gcgcctgaaa agctgacatt gtagcagcag ttctcgtgct cacttgcgcc gcgcgttgcg
     2581 cacgccgttg acctggcgca cgatgccgag cactgaggac agcctggccg aatcggtgac
     2641 ctctaccgtg aaggtcatcc aggccatgcc cttgacggtc acggtattga cgccgatcac
     2701 gttcatcttc tccttcgcga acacttcgga gatgtcgcgc agcaggccct ggcggtcgtt
     2761 ggcctccaca gccacgtcca ccggatagag cgccggcttg tcggtccgcg cgccccccca
     2821 ggcgacggcg atcacgcgct cggggttgcg ccccgacatc tggcggaagt tggagcaatc
     2881 gctgcgatgg atcgccacgc ccttgccgcg cgtcacgtag ccgctgatgg cgtcgggcgg
     2941 cgccggcttg cagcagcgcg ccagcgtggt gagcagcgat tcgacgccca ccaccagcac
     3001 accgcccttg ggtgccgctg cctcgccgcc gccgcgcgag aggcgctggg ccaatgcctc
     3061 gtccggactg ggcaagggct cggccggctt gaacagatgc tcgatgttct tgagcgagta
     3121 ctcgtccttg ccgatcacct cgaacaaggc atcggcattg cgaaagccga gctgggcggc
     3181 cagctcctcg agcttgaccg cggtgcggcc ctcgcgctgc agcagcttct cgaccgcctc
     3241 gcgcccgcgc gcgatggtct gctgctgcgc cagcgcattg aaccaggccc gcaccttggc
     3301 cctggcgcgc gtgctctgca gatagcccag ctcaggattc agccaatcga gcgaaggccc
     3361 gccctccttc agcgcgatga cttccacggt ctgcccgctg gcgagcggcg tattgagcgg
     3421 caccatcgcg ccgtccacct tcgcaccgcg gcagcggtgg ccgaggtcgg tgtgcaccgt
     3481 gtaggcgaag tccaccggcg ttgcgccggc gggcagctcc acgattgcgc cttgcggtgt
     3541 gaagacatag atgcggtcgt cgaacaggcc ctgctcgccg gcgcgaggct cgggcgcgcc
     3601 ggccgtcgcc aggtcatgct cccaggccag cagctggcgc agcacggcct tgcgcgcctg
     3661 agccacctgc tcctcaaagt cgcctgcggc gctcacgccg cgatagccgc gcgcgccggc
     3721 ctccttgtag gcccagtgcg cggcgacgcc gctctccgca tgctcgtgca tctcgcgcgt
     3781 gcggatctgg atctcgatcg cgcggccctc ctcgtcgagc accacggtgt gcagcgactg
     3841 gtagccgttg ggcttgggtt tggcgatgta gtcgtcgaac tcctcgggca gcgagcgcca
     3901 cagctcgtgc acgcggctga gcgctgcata gcaggcccgc acgtcgggca cgatgacgcg
     3961 cagcgcacgc acgtcgaaca cgcgctccag ggtcaggccc ttgccctgca tcttcttcca
     4021 gatgctgtac agatgcttgg ggcgcccctg cacttgcgcc tgcacgccgg cgcgccgcag
     4081 ctcgacctcc agcctcgcac gcgccgcttc caccacctgt tcacgctcga cccgcttctc
     4141 gtcgagctgg cgggcgatcg ccatgtaccg ctcgggctgc aggaagcgga aggacaggtc
     4201 ctccagctcc cacttgatct gccagatgcc aaggcggttg gccagcggcg cgaagacctg
     4261 cagcgactcg cgggcgagca cgccggggca aggccgcttg ctcgcggcgt agtagcgcaa
     4321 ggtctgcagg cgcgaagcca ggcgcatcag cacgacacgc aggtcgcgcg agaaggcgag
     4381 cagcatcttg cgcacccgct cgagctgctc ggcctgcacg gcgccgtcgc gctcggcagc
     4441 ctcgtcggcc agcgcgtcgc gggcactgcg ctgcacctgg accagcttgc gcgtgtgggc
     4501 gacgaggctc gcatagctct cgccgaaggc cttggcgacc atctcctccg gcttgttgag
     4561 aaagtcggcc gcatagacca gataggcggc cgcacgcatc gaaggcgcag ccccgatgcc
     4621 tcgcagaatg gccgcgacgc catcggcatg ggccagcgcg tcctcgccgg tgtccagccg
     4681 ctggccagcc agcagcggct gcgagaaggc cgccgccttg gccagcaagg cctcctccgc
     4741 ggcgggagcc tgctccgcct gcgcctcgcc cgccagttga acgatgggag cggcttgtgc
     4801 tgccgtctcc tgcaagcccg tcttcatggc ccggtcagga actccttgac gagtgccacc
     4861 tggtcggcag caagcagcgt cggcgcatgg ccgatgcccg gcagctccac cagcttggcc
     4921 ttggggccgc gccgcgtcat ggcctgcgcc gtgtcgcgcg acagcagatc ggattgctcg
     4981 ccgcggatca gcagcgtcgg gcagctgatc gcgtcatagc gctgccacag cgctgcctcg
     5041 ccggcggccg ccagctccgg cgtcacaccg ttgaaggcca atgcaatgcg cgggtcgtag
     5101 cgactgaccc agccgtcgcc atcgggcttg atctgcggct gcgtcagcgc cagccattgc
     5161 tcccgggtgt gcgggccgaa gcccttggag atgctccaca gatagtctgc cgcctcgtcg
     5221 acgctggcat agcgcaaagg cttgccgagg tagtcgccga tgcgcttgat cgaattgggc
     5281 tcgatcgtgg ggccgacgtc gttgagcacc aggcgccgga tcggcgtgtc gggcagcgag
     5341 gccatgccga tgccgatcag gccgcccatc gaggtaccga cccaa
//
LOCUS       DASUCY010000644         3534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_898105, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000644 DASUCY010000000
VERSION     DASUCY010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898105"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..120)
                     /locus_tag="VFV25_12750"
     CDS             complement(<1..120)
                     /locus_tag="VFV25_12750"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561263.1"
                     /note="catalyzes the formation of
                     3-deoxy-D-arabino-hept-2-ulosonate 7 phosphate from
                     phosphoenolpyruvate and D-erythrose 4-phosphate,
                     phenylalanine sensitive; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEY1394234.1"
                     /translation="MNAAAKSSTASEGWYALSEKTSQTDDERIQDVTPLPPPEH"
     gene            585..1724
                     /locus_tag="VFV25_12755"
     CDS             585..1724
                     /locus_tag="VFV25_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY1394235.1"
                     /translation="MLHPYCRKLLLIVVRALLPALFAGLWVLPAMAAATRVAFVNPGK
                     SDETYWVTATEAMQQAARSLGLELEVRYAERDHQRMLELVREIAQRPVERRPDYLVLV
                     NERRMGGEMLKLASAAGIKCLFAYNTLLPDDRAEYGAPRGRYPLWLGSIVPHAEDAGY
                     LTAKALIERGLREHRYAADGKLHMLALAGDHSTESSMLRDKGMRRAVGEHPKVVLDQL
                     VYADWRRDSARLAAQELFARYPNAALVWAGNDRMAFGAMDALQAAGGRPGVDRWFSGI
                     NTSSEAMQEVIAGRMEALAGGHFMAGAWALVMIYDHAHGRDFVADEGLELDKPMFALF
                     DAELAHRYLARFASGVPALDFRPYSKVLNPRLRRYDFGFAGLLHD"
     gene            1717..3054
                     /locus_tag="VFV25_12760"
     CDS             1717..3054
                     /locus_tag="VFV25_12760"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEY1394236.1"
                     /translation="MTDQPASDRPFRSLASRLVRGVVLVCLGCALLVMVLRGWLVWHV
                     ESESLARTTQDIAQSRLPLLSGALWDIQTQTAQAQVDEIARHPQVAGVRLVTVGGLSF
                     KAGAPLPAGKADVELAVPRPQGKGAPLGHLTIAFNHDFARAELARSFLRTLVAGSIIT
                     LIAYLALVRFLRREVSEPLRRIADYAGRLQPGALAPPLDYVRAERPWRDDIDLVAEGF
                     RTLHDGLQRYVSERDAALHALAQERDQLEATVLERTADLARLNQELLRLSRSDALTGL
                     PNRRAFDEHKLEELRRARRQRHPLTVIVADIDFFKLYNDRYGHSAGDDCLVRVAQCLA
                     GLFKRPGECVARLGGEEFAVLLPETELNEAGQQGERIRTAVADLGLVHEGTAPGVVSV
                     SVGVATLADHSQEGHSEEALASAFDLLFGRADAALYMAKGAGRNIVRVHPAPP"
     gene            complement(3149..>3534)
                     /locus_tag="VFV25_12765"
     CDS             complement(3149..>3534)
                     /locus_tag="VFV25_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEY1394237.1"
                     /translation="YAHVPMPRMTNTYMLAGDRDPAEIIASVKNGVFAANFGGGQVDI
                     TSGKYVFQCTEAYRIENGKIQYPVKGATIVGNGPDALTRVTLIGKDMRLDSGVGTCGK
                     EGQSVPVGVGQPTMRIDGLTVGGTA"
BASE COUNT          530 a         1192 c         1222 g          590 t
ORIGIN      
        1 gtgctccggc ggcggtagcg gcgtcacgtc ctggatcctc tcgtcgtcgg tctgcgaggt
       61 cttctcggac agcgcatacc aaccctcgct ggcggtgctg gacttggcgg cggcgttcat
      121 ggtttgcgtc tctccttgcg ggggcatgcg tcgatttcga tgaggcataa aaaaaaccgc
      181 cgggggaggc ccggcggttt tgggattcgg tcttggcgct ttgcttgctg ctcaggccct
      241 ggcctctcct ccgccggtgc ggtgcgagaa ccaaaagaag ccaaagtaaa agcgggaagc
      301 ggcgctcaga agcatgggcg caaatgtagc acgaggcttg tgcactgcgt caagcgggcg
      361 gggcctgagc ttcttgacga ttcccgagac aaccgatcgg gtaagcgccg gtatggcgcc
      421 ccgcaagcgg gacgatgcgg cggtgccgcc agacggtgcg agcccgacac gaaagcgtgc
      481 agattgccca gcgacgggga cgggattgcc gcagcgcaca agccatctgt tacaaacccc
      541 ggcgaggtgc cgataactcc tttacgccta ctcggttgcg atccatgctc cacccgtatt
      601 gccgcaagct gctgctcatc gtcgtccgcg ctctgttgcc ggccctgttc gccggcctct
      661 gggtgctgcc ggccatggcg gccgcgacgc gggtggcctt cgtcaacccc ggcaagtcgg
      721 acgaaaccta ctgggtcacc gcgaccgagg ccatgcagca ggccgcccgc agcctggggc
      781 tggagctgga ggtacgctat gccgagcgcg accatcagcg catgctggag ctggtgcgcg
      841 aaatcgcgca acgccccgtc gagcgccgcc cggactatct ggtgctggtg aacgagcggc
      901 gcatgggcgg cgagatgctc aagctcgcca gcgcagccgg catcaagtgc ctgttcgcct
      961 acaacaccct cctgcccgat gaccgcgccg agtacggtgc accgcgcggc cgctacccgt
     1021 tgtggctggg ctccatcgtg ccgcatgcgg aggacgcggg ctatctgacc gccaaggcgc
     1081 tgatcgagcg cggcctgcgc gagcaccgct acgccgcaga cggcaagctg cacatgctgg
     1141 cgctggccgg cgaccactcg accgagtcgt cgatgctgcg cgacaagggc atgaggcggg
     1201 ccgtgggcga gcatcccaag gtggtgctgg accagctcgt ctacgccgac tggcgtcgcg
     1261 actcggcccg gctggcggcc caggaactgt ttgcccgcta ccccaatgcg gcgctggtct
     1321 gggccggcaa cgacaggatg gccttcggcg ccatggatgc gctgcaggca gccggcggcc
     1381 gccccggcgt ggatcgctgg ttcagcggca tcaacacctc tagcgaggca atgcaggagg
     1441 tcatcgccgg gcgcatggag gcgctggccg gcggccattt catggccggt gcctgggccc
     1501 tggtgatgat ctatgaccat gcccatggcc gcgatttcgt ggccgatgag gggctggagt
     1561 tggacaagcc catgttcgcg ctcttcgacg ccgagttggc gcaccgctac ctcgcgcgct
     1621 ttgcgtccgg cgtgccggcg ctggacttcc ggccttacag caaggtcctc aaccccaggc
     1681 tgcgccgcta cgacttcggc tttgcaggct tgctgcatga ctgaccagcc ggccagcgac
     1741 agaccctttc gctcgctcgc ctcgcggctg gtgcgaggcg tggtgctggt ctgcctcggc
     1801 tgcgcgctgc tggtgatggt gctgcgcggc tggctggtct ggcatgtcga gagcgagagc
     1861 ctggcgcgca cgacgcagga catcgcgcag tcccgcctgc ctttgctgtc tggcgcgctg
     1921 tgggacatcc agacgcagac cgcccaggcc caggtcgacg agatcgcgcg ccacccgcag
     1981 gtggccggcg tgcgtctcgt cacggtgggc gggctgagct tcaaggccgg cgcgccgctg
     2041 cctgccggta aggcggatgt ggagctggcc gtgccccgcc cgcaaggcaa gggcgcgccg
     2101 ctggggcatc tgacgatcgc cttcaatcac gatttcgccc gtgccgagtt ggcgcgcagc
     2161 ttcctgcgca ccctggtcgc cggttccatc atcactctga tcgcctacct ggccctggtg
     2221 cgcttcttgc gccgcgaagt gagcgagccc ttgcgtcgga tcgccgatta cgcaggcagg
     2281 ctccagccgg gcgcactggc gccgccgctc gattacgtac gcgccgagcg gccctggcgc
     2341 gacgacatcg atctcgtcgc cgagggcttt cgcacgctgc acgatggctt gcagcgctac
     2401 gtcagcgagc gcgacgcggc gctgcacgcg ctggcgcaag agcgcgacca gctcgaagcc
     2461 acggtgctcg agcgcaccgc agacctggcc aggctaaacc aagagctgtt gcgcctgtcg
     2521 cgcagcgacg cgctgaccgg cctgcccaat cgccgcgcct tcgacgagca caagctggag
     2581 gaactgcggc gcgcccggcg ccagcggcat ccgctgaccg tgatcgtcgc cgacatcgac
     2641 tttttcaagc tctacaacga ccgctacggc cacagcgcgg gcgacgactg cctggtgcgc
     2701 gtcgctcaat gcctggccgg cttgttcaag cgccccggcg aatgcgtcgc ccgcctgggc
     2761 ggtgaggagt tcgccgtgct gctgcccgag accgagctga acgaagcggg gcagcagggt
     2821 gagcgtatcc gcacagcggt ggcggacctg ggactcgtcc acgagggcac ggcaccgggg
     2881 gtggtcagcg tcagcgtcgg cgtcgcgacc ctggccgacc actcacagga aggccacagc
     2941 gaggaagccc tggccagcgc cttcgacctg ctgtttggcc gggccgatgc ggcgctctac
     3001 atggccaagg gtgccgggcg caacatcgtg agggtgcatc cggcaccgcc ctagcgatag
     3061 ctcaggccgg cgctgcgccg ggccgctccc aagcaaaccg acaccctctc ggagggtggt
     3121 cgcggagtcc gcgaccgggt gtcgttgtct aagccgtgcc gcccaccgtc aggccgtcga
     3181 tgcgcatcgt cggctggccc acgcccaccg gcacgctctg gccttccttg ccgcaggtgc
     3241 ccacgccgct gtccaggcgc atgtccttgc cgatcagcgt gacgcgggtc agcgcgtccg
     3301 ggccgttgcc gacgatggtg gcgcccttga cggggtactg gatcttgccg ttctcgatgc
     3361 gataggcctc ggtgcattgg aacacatatt tgcccgaggt gatgtcgacc tggccgccgc
     3421 cgaaattggc ggcgaacacg ccgttcttca cggacgcaat gatttcggcg gggtcgcggt
     3481 cgcctgcgag catgtaggtg ttggtcatgc gcggcatcgg cacatgggca tagc
//
LOCUS       DASUCY010000645         2287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1010764, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000645 DASUCY010000000
VERSION     DASUCY010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010764"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VFV25_12770"
     CDS             complement(<1..423)
                     /locus_tag="VFV25_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEY1394238.1"
                     /translation="MEIFGIPLQALFGQLLLGLVNGSFYAMLSLGLAVIFGLLGIVNF
                     AHGALYMIGAYVAWLSLEKFGVNYWLALLLAPLVVGALGVIIERSLLKRLYKLDPLYG
                     LLLTFGLAQIAEGVFRDQFGVSGQSYSVPDLLTGATDLG"
     gene            complement(493..1695)
                     /locus_tag="VFV25_12775"
     CDS             complement(493..1695)
                     /locus_tag="VFV25_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY1394239.1"
                     /translation="MKKTLISMACALTLGFAVPAQAQISGDVVRIGFITDMSGLYSDI
                     DGQGGAEAIKMAIAEFGGAINGKKIELLVADHQNKADVAAAKAREWFDQQGVDVLIGG
                     TNSGANLAMAKVAGEKKKPFIVVGAASSALTNEQCSPYTVHYAYDTVALAKGTGNAVV
                     KAGGKSWFFLTADYAFGAALQNDTANVVKAAGGTVVGSVKHPLSASDFSSFLLQAQSS
                     KAQILGLANAGGDTINAVKAANEFGITKTMKLAGLLVFINDIHSLGLKTTQGMYLTDS
                     WYWNQNAETRTWARNFFDKMKRMPSSLQAGDYSAARTYLTAVKAIGSDDADKVLAQMH
                     KTKINDVFAKDGYVRPDGRMVHDMYLMQVKTPAQSTEPWDYYKVVETIKGDAAWTTKA
                     ESKCALWK"
     gene            complement(1751..>2287)
                     /locus_tag="VFV25_12780"
     CDS             complement(1751..>2287)
                     /locus_tag="VFV25_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY1394240.1"
                     /translation="SRKGSIKLHGQEAIDLPTHRIARLGVGYCPEERGIFSSLSTEEN
                     LMLPPVLTAGGMAVDRIYAMFPNLKERAASQGTRLSGGEQQMLAVARILRTGAKLLLL
                     DEISEGLAPVIVQKLAEMVVMLRQQGYTVVMVEQNFRFAAPLADRFMVMEHGRVIQEF
                     SQAELPQRINQLHEYLGV"
BASE COUNT          378 a          750 c          738 g          421 t
ORIGIN      
        1 gccgaggtcg gtcgcgcccg tcagcaggtc aggcaccgaa taggactggc ccgacacccc
       61 gaactggtcg cggaacacgc cttcggcgat ctgcgccagc ccgaaggtca gcagcaggcc
      121 atagagcgga tcgagcttgt acagccgctt gaggaggctg cgctcgatga tcacgcccag
      181 agcgccgacc accagcggcg ccagcagaag cgcgagccag tagttgacgc cgaacttctc
      241 caggctcagc caggccacgt aggcgccgat catgtagagc gcgccgtggg cgaagttcac
      301 gatgcccagc agcccgaaga tcaccgcaag gcccaggctg agcatcgcgt agaacgagcc
      361 gttgaccagc ccgagcagca gttgcccgaa aagagcctgc agcggtatgc cgaagatttc
      421 catcttgtgc ttgtttggcg caagcgcgcg gccgcagact tgaagctggc ggccgcgcag
      481 ctcaggctgg catcacttcc acagtgcgca tttcgattcc gccttggtcg tccacgcggc
      541 atcgcccttg atggtctcga ccaccttgta gtagtcccag ggctcggtgg actgcgccgg
      601 cgtcttcacc tgcatcaggt acatgtcgtg caccatgcgg ccgtcggggc ggacatagcc
      661 gtccttggcg aacacatcgt tgatcttggt cttgtgcatc tgcgccagca ccttgtcggc
      721 gtcgtcgctg ccgatcgcct tgacggcagt gaggtaggtg cgcgcggccg agtagtcgcc
      781 ggcctgcagc gacgagggca tgcgcttcat cttgtcgaag aagttgcgcg cccaggtgcg
      841 cgtctcggcg ttctggttcc agtaccagct gtcggtcagg tacatgccct gcgtggtctt
      901 caggcccaac gaatggatgt cgttgatgaa gaccagcagg ccggccagct tcatcgtctt
      961 ggtgatgccg aactcgttgg cagccttgac cgcgttgatg gtgtcgccgc cggcgttcgc
     1021 gaggccgagg atctgcgcct tgctcgattg cgcctgcagc aggaatgacg agaagtcgct
     1081 cgccgacagc ggatgcttga ccgaaccgac caccgtgccg cccgcggcct tcacgacgtt
     1141 ggccgtgtcg ttctgcagcg cggcgccgaa cgcatagtcc gcggtgagga agaaccagct
     1201 cttgccgccg gccttcacca ccgcattgcc ggtgcccttg gccagcgcga cggtgtcgta
     1261 ggcatagtgc acggtgtacg gcgagcactg ctcgttggtc agcgccgacg aggccgcgcc
     1321 gaccacgatg aagggcttct tcttctcgcc cgccaccttg gccattgcaa gattggcgcc
     1381 ggagttggtg ccgccgatca gcacgtccac gccctgctgg tcgaaccact cgcgcgcctt
     1441 ggcggcggcc acgtcggcct tgttctggtg gtcggccacc agcagctcga tcttcttgcc
     1501 gttgatcgcg ccgccgaatt cggcgatcgc catcttgatc gcctcggcgc cgccctggcc
     1561 gtcgatgtcc gaatacagcc cagacatgtc ggtgatgaag ccgatgcgga ccacgtcgcc
     1621 cgagatctgc gcctgggctg gcaccgcgaa gcccagcgtc agcgcgcagg ccatggagat
     1681 gagggtcttc ttcattgcta cctccttgtg tggatgttgc gagagactgt tgcgagagac
     1741 tgcgagcgcc tcagacgccc aggtattcgt gcagctggtt gatgcgctgg ggcagctccg
     1801 cctggctgaa ctcctggatc acgcggccat gctccatcac catgaagcgg tcggccagcg
     1861 gcgcggcgaa gcggaagttc tgctccacca tcacgacggt gtagccctgc tggcgcagca
     1921 tcacgaccat ctcggccagc ttctgcacga tgaccggggc caggccctcg gaaatctcgt
     1981 ccagcagcag cagcttggcg ccggtacgca ggatgcgcgc cactgcgagc atctgctgct
     2041 cgccgcccga cagccgcgtg ccctggctgg cggcgcgctc cttgaggttg gggaacatcg
     2101 catagatgcg gtctaccgcc atgccgccgg cggtgagcac aggcggcagc atcaggttct
     2161 cttcggtcga caggctggag aagatgccgc gctcttcggg gcaatagccc acgcccagcc
     2221 tcgcgatgcg atgcgtcgga aggtcgatcg cttcctggcc atgcagcttg atcgagccct
     2281 tgcggct
//
LOCUS       DASUCY010000646         2919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_271605, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000646 DASUCY010000000
VERSION     DASUCY010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271605"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VFV25_12785"
     CDS             <1..612
                     /locus_tag="VFV25_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein"
                     /protein_id="HEY1394241.1"
                     /translation="ASLIEALEPLSPQLSYLSARGLPLRHVDANGGTAGFLQAVADAA
                     PFAEVVLVHAPASELARMFAQRPPGVGAPRAVLLCDERPEAMTHAYAALKLLAARSGV
                     MVHDLLLGAAPRSARASLIAQRLARCADDFLGAVQHSWVRIDPAEPVGTMTSPALRRL
                     VEALLPAAWLPSQSGAFVDSAFDALMNSHPAAALPVQRVPLMN"
     gene            639..1352
                     /locus_tag="VFV25_12790"
     CDS             639..1352
                     /locus_tag="VFV25_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HEY1394242.1"
                     /translation="MYTAKGRLDINAMLQQYSPLVRRLAHQMIAKLPANVEIDDLIQV
                     GMIGLSDALSRFDTAQGVQFETFATQRIRGAMLDELRGSDYLSRGTRKQQRSIETAVH
                     KLEQKLGRAPQESEIAKEMGVTLAEYQDLLGKVRGTQLVYLEDMSGEDGDADYLDRHV
                     ADEGENPLSLLQDRRMREALVEAIKGLPEREQYVMSMYYEHDMNLKEIAAVLGVTESR
                     VCQLHSQTIARLRVKLREW"
     gene            1432..2787
                     /locus_tag="VFV25_12795"
     CDS             1432..2787
                     /locus_tag="VFV25_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEY1394243.1"
                     /translation="MFALFRQDRAESATSSAEPASGSNGADALAAVQAIAKHASLLGR
                     DAADVRGLIDDTTKVSARQAQSMAALAGQVGEVQRSQQMIAATTTGSLTAVSRAREAV
                     LSVGSEVAGIVDTLRQVAQAAGQITQIALQTRLVAFNASVEAKRAGTAGLGFGVVADA
                     VKDLSAKVEASSKEIMGTVSQLDTRIAALAREIQADAAQGRQSSFHHALADVEAGVAS
                     ITAAAEQSRSICGGLNQQMGTLESEMQQTTKALDTAMTRSEAFLKVSEHLIELVAECG
                     IETEDTPYIRAAQHAAAQIARLLEDSLRTDTVTQAELFDEQYKPLPNTAPQQHTTRYV
                     ELADRLFPQVQEKALELSPKVVFCVATDRNGYIATHNKKYCLPQRPGDGAWNTANSRY
                     RRIFNDRTGLASARNRRPFLLQTYRRDMGGGNFVLLKEAAVPISVAGRHWGCIRLAFQ
                     F"
BASE COUNT          499 a         1017 c         1005 g          398 t
ORIGIN      
        1 gcgagcctca ttgaagcgct ggagcccttg tcgccccagc tgagctacct gagcgcgcgc
       61 ggcctgccgc tgcgccacgt ggatgccaac ggcggcaccg ccggctttct gcaggccgtg
      121 gccgatgcgg cgcctttcgc cgaggtcgtg ctggtgcatg ccccggcttc cgagctggcg
      181 cgcatgttcg cgcagcgccc gccgggcgtg ggtgcgccgc gcgccgtgct gctgtgcgac
      241 gagcggcccg aggccatgac ccatgcctat gcggcgctca agctgctggc cgcgcgcagc
      301 ggcgtcatgg tgcacgacct gctgctgggc gccgcgccgc ggtcggcacg cgcctcgctc
      361 atcgcccaac gcctggcgcg ctgcgccgac gatttcctgg gcgcggtcca gcacagctgg
      421 gtgcgcatcg acccggccga gccggtcggc accatgacca gcccggcgct gcgccgcctc
      481 gtcgaggcgc tgctgccggc ggcctggctg ccctcgcaga gcggcgcctt cgtcgattcc
      541 gcgttcgacg cgctgatgaa cagccatccc gcggcggcgc tgccggtgca gcgcgtgccg
      601 ctcatgaatt gagacgacaa gacggaagag agccgagcat gtacaccgcc aagggccgcc
      661 tcgacatcaa tgccatgctg cagcagtaca gccccctggt gcgccgcctg gcgcaccaga
      721 tgatcgccaa gctgccggcc aatgtggaga tcgacgacct gatccaggtc ggcatgatcg
      781 gcctgagcga cgctttaagc cgcttcgata ccgcgcaggg cgtgcagttc gagaccttcg
      841 cgacgcagcg catccgcggc gccatgctcg acgagctgcg cggctccgac tacctgtcgc
      901 gcggcacgcg caagcagcag cgctccatcg agacggcggt gcacaagctg gagcagaagc
      961 tcggccgcgc cccgcaggaa agcgagatcg ccaaggaaat gggcgtcacg ctggccgaat
     1021 accaggatct gctcggcaag gtgcgcggca cccagctcgt ctacctcgag gacatgagcg
     1081 gcgaggacgg cgatgcggac tacctggacc gccatgtcgc cgacgaaggc gagaacccgc
     1141 tgtcgctgct gcaggaccgc cgcatgcgcg aggccctggt ggaggcgatc aagggcctgc
     1201 cggagcgcga gcagtacgtg atgagcatgt actacgagca cgacatgaat ctcaaggaga
     1261 tcgcggcggt gctgggcgtg accgaatccc gggtttgcca gctgcacagc cagaccatcg
     1321 cgcgcctgag ggtcaagctg cgcgaatggt gagccgataa caggacatag cccggagccc
     1381 ggcgccggat tgccgagtgc gccgcggcca gccaaggagg cacataccgc gatgtttgcc
     1441 ttgttccgtc aagaccgcgc cgaatccgcg acgtcctccg ccgagcccgc atcgggcagc
     1501 aacggcgccg acgcgctggc ggcggtgcag gccattgcca agcacgcatc gctgctgggc
     1561 cgcgacgcgg ccgacgtgcg cgggctgatc gacgacacca ccaaggtctc ggcccgccag
     1621 gcccagtcca tggcggcgct ggccggccag gtgggcgaag tgcagcgctc gcagcagatg
     1681 atcgcggcca ccacgacggg cagcctcacg gccgtctcgc gggctcgcga ggcggtgctc
     1741 agcgtcggca gcgaagtggc cggcatcgtc gatacgctgc gccaggtggc gcaagccgcc
     1801 ggccagatca cgcagatcgc cttgcagacg cgcttggtgg ccttcaatgc ctcggtggag
     1861 gccaagcgcg ccggcacggc cggcctcggc ttcggcgtgg tggccgacgc ggtgaaggac
     1921 ctctccgcca aggtcgaagc ctcgtcgaag gagatcatgg gcacggtgag ccagctcgac
     1981 acccgcatcg ctgcactggc ccgcgagatc caggccgacg cggcacaggg ccggcaaagc
     2041 agcttccatc atgcgctggc cgatgtggag gccggggtcg caagcatcac ggccgctgca
     2101 gagcagagcc gcagcatctg cggcgggctc aatcagcaga tgggcacgct ggaatccgag
     2161 atgcagcaga ccacgaaggc gctcgacacc gcgatgacgc gcagcgaggc cttcctgaag
     2221 gtgagcgagc acctcatcga gctggtggcc gaatgcggca tcgagaccga ggacacgccc
     2281 tacatccgcg cggcacaaca cgcagctgcg cagatcgccc ggctgctcga agattccctg
     2341 cgcaccgaca ccgtcacgca ggccgagctg ttcgacgagc agtacaagcc gctgccgaat
     2401 accgctccgc agcagcacac cacgcgctat gtcgagctgg ccgaccggct ctttccgcag
     2461 gtgcaggaga aggcgctgga gctctcgccc aaggtggtgt tctgcgtcgc gaccgaccgc
     2521 aacggctaca tcgccacgca caacaagaaa tactgcctgc cgcagcgccc gggcgacggg
     2581 gcctggaaca cggccaacag ccgctaccgc cgcatcttca acgaccgcac cggactcgct
     2641 tcggcgcgca accggcggcc tttcctgctg cagacctatc gccgcgacat gggcggcggc
     2701 aacttcgtgc tgctgaagga ggccgccgtg ccgatcagcg tcgccggccg ccattggggc
     2761 tgcatccgcc tggctttcca gttctgaagc agacagcgca gcgccggctc cttctcccgc
     2821 tcgcgggaga aggcggggga tgaggggtcg cgaggcctcg cttcatgtga gtcgcgcgaa
     2881 gcgcggcgga gcaaaggtca ggcctggatg gatccgccg
//
LOCUS       DASUCY010000647         4953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_351705, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000647 DASUCY010000000
VERSION     DASUCY010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4953
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351705"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="VFV25_12800"
     CDS             <1..876
                     /locus_tag="VFV25_12800"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY1394244.1"
                     /translation="EEAIALVSSLAADVGVKVGISSSPQGAAAADGYWVLADRVRLRQ
                     ILVNLLNNAVKYNRPGGQVLLSHECRNDWCQIVVADTGKGMTTEQVNRLYEPFNRLGA
                     ELSGIEGTGIGLVLTRHLVELMGGDITIESTPGVGTVATVQLQCASTPANTMPAPYVP
                     SQHGTLEESTLHVLYAEDNEVNVELVRQVAALRPAVDLRVAANGTLALEMARLDPPDL
                     MLVDMHLGDMTGMDLARALRREPATASIHLVALSADALPEQINAALSWGFDGYLTKPI
                     DFRELLRVLDNHISA"
     gene            902..1351
                     /locus_tag="VFV25_12805"
     CDS             902..1351
                     /locus_tag="VFV25_12805"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEY1394245.1"
                     /translation="MFVRALLAFLALPGIVAFAIPVAWLLASSHTRLTQPLGLLPLIC
                     GTAALLWCVRDFYVAGRGTLAPWAPPQHLVVVGLYRYTRNPMYVAVTLILLGWAAAFE
                     LPGLFFYALVVAVAFHLRVVFGEEPWLAHTHGAQWHEYARRVPRWLI"
     gene            complement(1427..2608)
                     /locus_tag="VFV25_12810"
     CDS             complement(1427..2608)
                     /locus_tag="VFV25_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEY1394246.1"
                     /translation="MAAPAVSTVPGMPPVVDANNLYSEIGATHLSAAVQGQLERVYVP
                     NLRSNDVYVIDPATAKVVDRFKVGVGPQHVVPSWDLKTLWVANNAERRTDGSLTPIDP
                     RSGKPGKAISVDDPYNMYFTPDGKSAIVVAEARRRLDFRDPQTMAPQYSIDTPQCGGI
                     NHADFSIDGRYALFTCEFDGSVTKIDLVNRKVLGYLKLTMPSTRYKEAPRRATAAAAQ
                     PFEPGASEVCSVTKGMPQDIRISPDGKRFYIADMEADGVHIVDGESFKQVGFISTGLG
                     THGLYPSRDGKSLYVANRGTHKIHGAPKGPGGVTVVDFATEKVTAQWPIPGGGSPDMG
                     NVSADGKTLWLSGRYDDVVYRIDTSNGAVTQIKVGQEPHGLTVWPQPGRYSLGHTGNL
                     R"
     gene            complement(2763..3611)
                     /locus_tag="VFV25_12815"
     CDS             complement(2763..3611)
                     /locus_tag="VFV25_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEY1394247.1"
                     /translation="MRSDGKASATTAWLARAAAACLFVATFLLPPAQAAAPSNRIEDL
                     TWTELRERIAAGATTVLVPIGGTEQNGPYMVLGKHNTRARILADEIAQRLGNAVVAPV
                     VAYVPEGSIHPPVAHMRFTGTISIPESTFEALLEATARSFKQHGFRDIVFLGDHGGYQ
                     KSEERLASKLNREWAADPACRVHALLDYYRVTQTAYVAALKSRGHSEAEIGTHAGLAD
                     TSLALAVDPALVRGDALAHSGKLSEREGVYGDPRRASAELGQLGVQQIIQTSVAAIQA
                     MTRERR"
     gene            complement(3699..4271)
                     /locus_tag="VFV25_12820"
     CDS             complement(3699..4271)
                     /locus_tag="VFV25_12820"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEY1394248.1"
                     /translation="MPTTLSLLNQVRTQARANRLANHRLHEAMAALSDADFHAPRVSF
                     FPSLAQTLNHLLAVDRYYLAALRREADMEAQYRSFQPAVTLAELAPRQRAFDEELIAY
                     CDAIDEAQLGDEIVLDRGGDEKPRERVAHVLAHLFMHQTHHRGQVHAMLAGTGVEPPQ
                     LDEFLMTSDAPLRSRDIAALGWSESQLFGS"
     gene            4485..4832
                     /locus_tag="VFV25_12825"
     CDS             4485..4832
                     /locus_tag="VFV25_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014896139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEY1394249.1"
                     /translation="MLYKGSCHCGRIAFQVEGTLDGAMSCNCSICQRKGSLLWFVPRE
                     RLQLLTPDENASTYTFNKHVIKHRFCPNCGMHPYGEGVDRKGNAMAAINIRCLEDVEI
                     EKVPVTHYDGRSH"
BASE COUNT          771 a         1655 c         1672 g          855 t
ORIGIN      
        1 gaagaggcga ttgccctggt ttcgtccctc gccgccgacg tcggcgtcaa ggtcggcatc
       61 tcttcgtcgc cgcagggggc agcagcagcc gacggatact gggtcctggc cgaccgcgtg
      121 cgcctgcgcc agatcctggt caacctgctg aacaacgcgg tcaagtacaa ccgcccgggc
      181 ggccaggtat tgctgagcca cgagtgccgc aacgactggt gccagatcgt cgtcgccgat
      241 acgggcaagg gcatgacgac cgagcaggtg aaccgcctct acgaaccctt caatcgcctc
      301 ggcgccgagc tgtcggggat cgaaggcacg ggcatcggcc tggtgctcac gcgccatctg
      361 gtcgagctga tgggcggcga catcaccata gaaagcacgc ctggtgtggg aaccgtcgcc
      421 accgtccagc tgcaatgcgc gagcacgccc gccaacacca tgcccgctcc ctatgtcccc
      481 agccagcatg gcacgctgga agaaagcacg ctgcacgtgc tctatgccga ggacaacgaa
      541 gtcaacgtcg agctggtgcg gcaggtggcg gcgctacggc cggccgtcga cctgcgcgtg
      601 gccgcgaacg gcacgctggc gctcgagatg gcgcggctgg acccgcccga tctcatgctc
      661 gtcgacatgc atctgggcga catgaccggc atggacctgg ctcgcgcact gcgccgcgag
      721 ccggcgacgg ccagcatcca cctggtggcc ttgtcggccg acgcgctgcc ggagcagatc
      781 aacgccgcgc tgtcctgggg ctttgacggc tatctgacga agccgatcga ctttcgcgag
      841 ctgctgcggg tgttggacaa ccacatctcg gcgtgactgg ctatcgaccg gagttgagcc
      901 tatgttcgtg cgcgccttgc tcgcgttcct cgcgctgccg ggcatcgtgg ccttcgcgat
      961 ccccgtcgcc tggctgctcg cttcctcgca cacccggctc acgcagccgc tgggcctgct
     1021 gcctctgatc tgcggcaccg cggcgctgct gtggtgcgtg cgggatttct atgtggcggg
     1081 gcgcggcacg ctggcgccct gggcgccgcc tcagcacctg gttgtcgtcg gcctgtatcg
     1141 ctacacgcgc aaccccatgt acgtcgccgt caccctcatc ctgctcggct gggcagcagc
     1201 gttcgaactg cccggcctgt tcttctacgc cctcgttgtc gccgtcgcgt tccacctgcg
     1261 cgtcgtgttc ggcgaggagc cctggctggc gcacactcac ggcgcccagt ggcacgaata
     1321 tgcgcgccgc gtcccgcggt ggctcatttg actgtgcacc ggctgcctgt tcggcaacgc
     1381 aaacgaaggc cttgcgcctg cgtcggccgt aggcaagtag gaaggctcag cgcagattgc
     1441 ccgtatgccc cagcgaatag cgcccgggct gcggccatac ggtcaggcca tgcggctcct
     1501 gaccgacctt gatctgcgtg acggcaccgt tgctcgtgtc gatgcggtag accacatcgt
     1561 cgtagcggcc cgacagccac agcgtcttgc cgtcggcgct gacattgccc atgtcggggc
     1621 tgccgccgcc cgggatgggc cattgcgccg tgaccttctc ggtggcgaag tcgaccaccg
     1681 tgacgccgcc cggccccttc ggagcgccat ggatcttgtg cgtgccgcgg ttggccacgt
     1741 agaggctctt gccgtcgcgg ctcggataga ggccgtgggt gccgaggccg gtggagatga
     1801 agccgacctg cttgaaggac tcgccgtcga cgatgtggac gccgtcggcc tccatgtcgg
     1861 cgatgtagaa gcgcttgcca tccggcgaga tgcggatgtc ctgcggcatg cccttggtga
     1921 cggagcagac ctcgctcgca ccgggctcga agggttgcgc ggccgcggca gtagcgcggc
     1981 gcggcgcctc cttgtagcgc gtgctgggca tcgtcagctt gaggtagccc agcaccttgc
     2041 ggttgaccag gtcgatcttg gtcacgctgc cgtcgaactc gcaggtgaag agcgcgtagc
     2101 ggccgtcgat ggagaagtcg gcgtggttga tgccgccgca ttgcggcgtg tcgatcgaat
     2161 actgcggcgc catggtctgc gggtcgcgga agtcgagccg gcggcgcgct tcggccacga
     2221 cgatggcgga cttgccgtcc ggcgtgaaat acatgttgta ggggtcgtcg accgaaattg
     2281 ccttgccggg cttgccgctg cgtggatcga tgggcgtcag gctgccgtcg gtgcgccgct
     2341 cggcattgtt ggccacccac agcgtcttca gatcccagga cggcaccacg tgctgcgggc
     2401 cgacgccgac cttgaagcgg tcgaccacct tggcggtggc cgggtcgatc acgtagacgt
     2461 cgttggagcg caggttgggc acgtagacgc gctccagctg gccctgcacc gccgcgctca
     2521 ggtgcgtggc gccgatctcg ctgtagaggt tgttggcgtc caccaccggc ggcatgccgg
     2581 gcacggtgct cacggcgggg gctgccatcg gcgcggaagc cgaggtcgcc gccagaaccg
     2641 cagtggcaac acaggcgcca ccagcggccg ccagcgccag gctgcggccc aggacaggct
     2701 tgatcttttc cttgatgctc atgatgtggt ctctgttcct ctgacggaca tccgtgattg
     2761 cttcagcggc gctcgcgcgt catcgcctgt atcgcagcca cggaggtctg aatgatctgc
     2821 tgcacgccca attgtcccag ctcggccgag gcgcggcggg ggtcaccata cacgccttcg
     2881 cgctcgctca gcttgccgga atgggccagc gcatcgccgc gcacgagggc cgggtcgacg
     2941 gccagcgcca gcgaggtgtc ggccaggccg gcatgggtac cgatctcggc ctcgctgtgg
     3001 ccgcggctct tcaaggcggc gacataggcg gtctgcgtga cgcgatagta gtcgagcagg
     3061 gcatgcacgc ggcaggccgg atcggctgcc cattcgcggt tgagcttgct cgccaggcgc
     3121 tcttcgcttt tctggtaacc gccatggtca ccaagaaaca cgatgtcacg aaatccatgc
     3181 tgcttgaagc tgcgcgcggt ggcctcgagc agcgcctcga aggtcgactc cggaatcgag
     3241 atcgtgccgg tgaaccgcat gtgcgccact ggcgggtgga tggacccctc gggcacataa
     3301 gccacgaccg gcgcgacgac cgcattgccc aggcgctggg cgatctcgtc ggccaggatg
     3361 cgcgcgcgcg tgttgtgctt gcccagcacc atgtagggcc cgttctgctc ggtgccgccg
     3421 atgggcacca gcaccgtggt cgcgccggcg gcgatgcgct cgcgcagctc ggtccaggtg
     3481 aggtcttcga tgcggttgct cggcgcagcc gcttgcgcag gcgggagcag gaaagtcgct
     3541 acgaacagac aggccgccgc agcccgggcc agccaagccg tcgtcgcgct ggccttgcca
     3601 tccgatctca tcgctgaatt catcgccatc cttgcaggct ggttccgcgc ccgctcgcgc
     3661 gagtatgtcc cagcccggag agcccgcctt gatggcgatc aggagccgaa cagctgcgac
     3721 tccgaccagc ccagggccgc aatgtcgcgg ctgcgcaggg gcgcgtcgct agtcatcagg
     3781 aattcatcga gctgaggcgg ctcgacgccg gtgcccgcca gcatcgcgtg gacctggccg
     3841 cggtgatggg tctggtgcat gaacaaatgc gccagcacat gggcgacacg ctcacgcggc
     3901 ttctcgtcgc cgccgcggtc cagcacgatc tcgtcgccga gctgtgcctc gtcgatcgca
     3961 tcgcaatagg cgatcagctc ctcgtcgaag gcgcgttgcc gcggcgccag ctccgccagc
     4021 gtcaccgccg gctggaagct ccggtactgc gcctccatgt cggcctcacg gcgcagcgcc
     4081 gcgaggtagt agcggtccac cgcgaggagg tggttgagcg tttgcgccag gctggggaag
     4141 aagctcacgc gcggcgcatg gaagtcggca tcgctcaagg ccgccatcgc ctcgtgcagg
     4201 cgatggttgg ccaggcgatt ggcgcgcgcc tgggtgcgga cttggttcaa caggctgagg
     4261 gtcgtcggca tgctgggatt cccgtgtcgt ttcgagcagg cttgccggcg gagccgcggc
     4321 aaccacaacc agacgcccgc atcgcctgcg caccatggta gccatctcgg gcgcggccat
     4381 gcaacagcgc cgttggccag tagctgccgc cctttgaatg gggaaggcct gcgggtagag
     4441 tccatgatgc cctcgacgcc accccagttc atcggagacg acacatgctc tacaagggaa
     4501 gctgccactg cgggcgcatc gcgttccaag tcgaagggac gctcgacggt gcgatgtcat
     4561 gcaactgttc catctgccag cgcaagggct cgctgctgtg gttcgttccc cgcgagcgcc
     4621 ttcagctcct cacgcccgac gagaacgcga gcacctacac gttcaacaag catgtcatca
     4681 agcaccgctt ctgcccgaac tgcgggatgc acccttacgg cgagggcgtc gaccgcaagg
     4741 gcaatgccat ggcggccatc aacattcgct gcctcgagga cgtcgaaatc gaaaaggttc
     4801 cggtcactca ctacgacggc cggtcacact gaatgcgata acacccagat cgctcacgca
     4861 gcgggtctga cacccgcact gagcgagcgc ggagagcgtg agttccaaag cggccgacgc
     4921 gatcccttcc gggggggcga ttgacatgcc agg
//
LOCUS       DASUCY010000648         2180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_705442, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000648 DASUCY010000000
VERSION     DASUCY010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705442"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..1296
                     /gene="clsB"
                     /locus_tag="VFV25_12830"
     CDS             4..1296
                     /gene="clsB"
                     /locus_tag="VFV25_12830"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HEY1394250.1"
                     /translation="MREIDATLARREALSWYSLPRAVFSGGNDVELLRGGDALFPAMH
                     RAIAQATRDIWLATYIFHDDEAALGLADALVAAAARGVRVRVVIDGFGSGQSLATVHA
                     RLSKGGVAVAVFRPVQGWWSWLLPGQLRRLHQKLCVVDGREAFVGGINLIDDRLDLHH
                     GWQATPRLDYAVRLLGAVVRPVEQTARAMWTRAWLGRDWRDEIAALARRPHRLLRARK
                     LLRRLRTSKWAELPAGSADSNPVRVAFVVRDNLRQRRTIERSYVDAIHAARESVDIVC
                     PYFYPGHAFRRALREAARRGVRVRLLLQGRWDYRMAELAAHALYEELLGRGVYIHEYQ
                     PAYLHAKVAVVDADWATVGSSNIDPLSLLVNLEANVVVRDRAFTADLRAEIEAALADA
                     RRITAADGESGRFGLLRRALVAWCARVYLRVAGASGQY"
     gene            complement(1310..1693)
                     /locus_tag="VFV25_12835"
     CDS             complement(1310..1693)
                     /locus_tag="VFV25_12835"
                     /inference="COORDINATES: protein motif:HMM:NF017470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HEY1394251.1"
                     /translation="MTTPAPNRFDEPLSPAQILFSFQGRIPRKIFWLYGVLGSLLFAL
                     FLGLVLGIAGFEDYTIDAIINLLLIWPGLAIMVKRWHDRDKSGWWVLINLIPVIGFVW
                     TLVEGGFLRGSNGPNRYGEDLTGRV"
     gene            1919..>2180
                     /locus_tag="VFV25_12840"
     CDS             1919..>2180
                     /locus_tag="VFV25_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1394252.1"
                     /translation="MNFFALRAAISGAVLLAWSAAALAQAPSSAVPAASEPATAYSGH
                     GAASVAPEVIEKFRPKPLPEDISRRIQSLLDLRAPGGGRLTPD"
BASE COUNT          323 a          749 c          755 g          353 t
ORIGIN      
        1 cgcatgcgcg agatcgacgc caccctggcc cgacgtgaag ccctgagctg gtattcgctg
       61 ccgcgcgccg tgttcagcgg cggcaacgac gtggagctgc tgcgcggcgg cgacgcgctg
      121 tttccggcca tgcaccgggc catcgcgcag gcgacgcgcg acatctggct ggcgacctac
      181 atctttcacg acgacgaggc ggcgctggga ttggccgacg ccctggtggc cgcagcggcg
      241 cgcggcgtgc gcgtgagggt cgtgatcgac ggcttcggct ccgggcagtc actcgccacc
      301 gtgcatgccc gcctgagcaa gggcggcgtc gcggtggccg tgttccgccc cgtgcagggc
      361 tggtggagct ggctgctgcc cggccagctg cgccgcctgc atcaaaagct ttgcgtggtc
      421 gacgggcgcg aggccttcgt cggcggcatc aacctcatcg acgacaggct ggatctgcat
      481 cacggctggc aggcgacgcc tcgcctcgac tatgccgtgc gactgctcgg cgccgtcgtg
      541 aggccggtgg agcagaccgc ccgcgcgatg tggacccggg cctggctcgg ccgcgactgg
      601 cgcgacgaga tcgccgcact ggcgcgccgc ccgcatcggc tgctgcgcgc gcgcaagctg
      661 ctgcggcgcc tgcgcacctc gaagtgggcc gagctgccgg ccggcagcgc cgattcgaac
      721 ccggtgcgcg tcgccttcgt ggtacgcgac aacctgcgcc agcgccgcac catcgagcgc
      781 agctatgtcg acgcgataca tgcggcgcgc gagagcgtgg acatcgtctg cccctatttc
      841 tatcccggcc atgccttccg tcgtgccttg cgcgaggcgg cgcggcgcgg cgtgcgcgtg
      901 cggctgctgc tgcaagggcg ctgggactac cggatggctg aactggccgc ccatgcgctc
      961 tatgaagagc tgctgggtcg cggcgtgtac atccacgaat accagccggc ctacttgcat
     1021 gcgaaggtgg cagtggtgga tgccgactgg gccaccgtgg gcagctccaa catcgatccg
     1081 ctgtcgctgc tcgtcaacct ggaagccaat gtggtggtgc gcgaccgcgc cttcaccgcc
     1141 gacctgcgcg cagagatcga ggcggccctc gccgatgcgc gccgtatcac agctgccgac
     1201 ggcgagagcg gccgcttcgg cctgctgcgc cgcgccctcg tggcctggtg cgcgcgggtg
     1261 tacctgcgcg tggccggagc ctctgggcag tattgaaccc ggcgggcgat cagacgcgtc
     1321 cggtcagatc ttcgccgtag cgattggggc cgttgctgcc gcgaaggaag ccgccttcga
     1381 ccagggtcca gacgaagccg atgacgggga tcaggttgat cagcacccac cagcccgact
     1441 tgtcgcggtc gtgccagcgc ttgaccatga tcgcaagccc cggccatatc agcagcaggt
     1501 tgatgatcgc gtcgatcgtg tagtcctcga agccggcaat gcccagcacc aggccgagaa
     1561 agagcgcgaa cagcaggctg cccagcacgc catagagcca gaagatcttg cgcggtatgc
     1621 ggccctggaa gctgaacagg atctgcgcag ggctcagcgg ctcgtcgaag cggtttgggg
     1681 caggcgtggt cattcttgtt cctggcgatg gccgcacgcg cggtggtgct tgtcgaaaac
     1741 ggcggcgcgg cgcgcaagga tacgccggcc ggcggggctt gttcgcgctg gcgtccctcg
     1801 cacgactatc cctcgaggca ggtcaagcgc atcaatacct agactgacaa caagcttgca
     1861 atagcagaat gcatcggcgc acacccgcgc tgtcatcgcc aaagcctgga gaccctcctt
     1921 gaacttcttt gccctgcgcg ccgccatcag cggcgctgtc ttgcttgcct ggtcggccgc
     1981 tgcgctggcc caggctccgt cgtctgccgt gccggcggca tccgagcctg caactgccta
     2041 cagcggccac ggcgccgctt cggttgcgcc cgaagtgatc gagaaattcc gccccaagcc
     2101 gctgccggag gacatttcgc ggcgcatcca atcgctgctg gacttgcgcg cccccggcgg
     2161 cggccggctc acgccggacg
//
LOCUS       DASUCY010000649         4071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_304006, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000649 DASUCY010000000
VERSION     DASUCY010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304006"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..1195)
                     /locus_tag="VFV25_12845"
     CDS             complement(44..1195)
                     /locus_tag="VFV25_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY1394253.1"
                     /translation="MTTPVLQSRLPRVGTTIFSVMSALAQQHGAVNLGQGFPDFDCDP
                     RLVDEVTRAMRAGHNQYPPMPGVPGLREAIAAKIAALYGHGYDPGTEITVTAGATQAI
                     ITAVLAVVHPGDEVIVLEPCYDSYVPNIELAGGKAIHVPLTPGSFRPDFERIAAALSP
                     RTRAIIINTPHNPSATAWSEAEMDRLAQLLAPTDVLVISDEVYEHMVYDGRLHQSVAR
                     HAALAERSLLVSSFGKTYHVTGWKVGYVAAPAPLMAEFRKVHQFNVFTVNTPVQHALA
                     AFMADPAPYLQLPAFYAAKRELFREGLKRTRLRLLPCEGSYFQCVDYSAVSDMSEADF
                     CRWLTSEIGVAAIPLSAFYEGGYEQRIVRFCFAKREDTLHSALDRLARL"
     gene            complement(1200..2153)
                     /locus_tag="VFV25_12850"
     CDS             complement(1200..2153)
                     /locus_tag="VFV25_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HEY1394254.1"
                     /translation="MNLHQFRFVQEAARRNLNLTETAKALYTSQPGISKAILELEEEL
                     GVDIFARHGKRLKRITEPGQQVLKCIEIIMREVGNLKRIGEEFSKQDAGTLSIATTHT
                     QARYVLPTPVAALRKRYPKVSVSLHQGTPEQVARMLLDESAEIGMATQSLLNFDELVT
                     LPCYEWQHVVVMPAQHPLAQVERLTLDQLAAEPLISYHPSFTGRPRIDAAFEHRRLKP
                     SFVLEAIDSDVIKTYVRLGLGVGIVAEMAVREDPSFGPDGDLIWRPVGHLFGQNVTHV
                     AFKRGAYLRNFVYAFAELVSDRLTRNLIVRAMGGEADSYEL"
     gene            complement(2158..2562)
                     /locus_tag="VFV25_12855"
     CDS             complement(2158..2562)
                     /locus_tag="VFV25_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HEY1394255.1"
                     /translation="MMSQAGVLLFAHGARDPAWAIPFEAVATRLREHAPDVEVRLAFL
                     EFMQPELRAAAEELVRSGCRRVCIVPLFLGAGGHVRKDLPELLASLQADHPQVRWTLA
                     GAIGEQPQVIAAMAEAALALSTTTTTTNNSKA"
     gene            complement(2559..3665)
                     /gene="lptG"
                     /locus_tag="VFV25_12860"
     CDS             complement(2559..3665)
                     /gene="lptG"
                     /locus_tag="VFV25_12860"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEY1394256.1"
                     /translation="MRTVRRLLYTDVLSAVFFVAAAFLSLFFFIDIVDALENVGDHGY
                     TVYHALLYCLLELPGHIYELLPIAILIGAIYAMARLAQSSEYTILRTGGLSPGRALSL
                     LASLGLCFGIFTFVVGDFIAPAAEREATLLQARFKGGLSLERTGAWLKDSRHMSDGEH
                     NFSINVGRATADGEFHDIRIFEFGPDGRLIQRIAAQSGRVGGAGSWQLQNVDVTHWKL
                     DEAQPAPRGEALAQMDWPTGLTMRVVSAAVLPLNTMTTVSLFSYMSHLSTQEQAAQRY
                     EIQFWKKALYPFACLVMMSLALPFAYLHSRSGGLSFKVFGGIMLGISFVLLNNISNHL
                     GLLQNWTPWIAAAAPSAIYLLLSLAAFSWLVRYR"
     gene            complement(3671..>4071)
                     /locus_tag="VFV25_12865"
     CDS             complement(3671..>4071)
                     /locus_tag="VFV25_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEY1394257.1"
                     /translation="VGENVSARAEELLPRARSTLDLMSQPSPRAQGELVWRLGLAMGG
                     INLLLLGIGLSAGNPRRGGSWNLLYALMAFIVYYNLLNLSQAWVASGRLSLGACLALV
                     HGGTFVLALALLWWRDSGSARFAPRLRMGS"
BASE COUNT          685 a         1294 c         1447 g          645 t
ORIGIN      
        1 agaagctggg gttgagtcag attgtgtcac tgggccgggt gcattacagg cgcgccaatc
       61 ggtccagggc actgtgcagc gtgtcctcgc gcttggcgaa gcagaagcgc acgatgcgct
      121 gttcgtagcc gccctcgtag aaggccgaca gcggaatggc cgcgacgccg atctcgctcg
      181 tcagccagcg gcagaaatcg gcctcgctca tgtcgctcac ggccgagtag tccacgcatt
      241 ggaagtagct gccttcgcag ggcagcaggc gcagtcgcgt gcgcttcaag ccctcgcgga
      301 acagctcgcg cttcgcggcg tagaaggccg gcagctgcag atagggtgca gggtcggcca
      361 taaaggccgc cagcgcgtgc tgcaccggcg tgttgaccgt gaagacgttg aactggtgca
      421 ccttgcggaa ctcggccatc aagggagccg gtgcggccac gtagccgacc ttccagcccg
      481 tcacgtgata ggtcttgccg aagctggaca cgagcaggct gcgctcggcc agggccgcat
      541 ggcgggccac gctctggtgc aggcggccgt cgtagaccat gtgctcgtag acctcgtcgg
      601 agatcacgag cacgtcggtg ggcgcgagca gctgcgccag ccggtccatt tccgcctcgc
      661 tccacgcggt ggcgctgggg ttgtgcggcg tgttgatgat gatggcgcgc gtgcgcggcg
      721 acagcgcagc ggcgatgcgc tcgaaatcgg gccggaagct gccgggcgtc agcggcacgt
      781 ggatcgcctt gccgccggcc agctcgatgt tgggcacata gctgtcgtag cagggctcca
      841 gcacgatgac ttcgtcgccg ggatgcacca ccgcgagcac cgctgtgatg atggcctggg
      901 tcgcgccggc ggtgacagtg atctcggtgc ccgggtcgta gccgtggcca tagagcgcgg
      961 cgatcttggc cgcgatggct tcgcgcaagc ccggcacacc gggcatcggc ggatattggt
     1021 tgtggccggc gcgcatcgca cgcgtcactt catcgaccag gcgcgggtcg caatcgaaat
     1081 ccggaaagcc ctggcccaga ttgacggcgc catgctgctg ggcgagggcc gacatcacgc
     1141 tgaagatggt cgtgcccacg cgcggcaggc ggctttgcag gacgggggta gtcattggct
     1201 cagagttcgt aggagtccgc ctcgccgccc atggcccgca cgatgaggtt gcgggtcagc
     1261 cggtcggaca ccagctcggc gaaggcgtag acgaaattgc gcaggtaggc gccgcgcttg
     1321 aaggcgacgt gggtcacgtt ttgtccgaac aggtggccca ccggccgcca gatgagatcg
     1381 ccgtcggggc cgaagctcgg gtcttcgcgc accgccattt cggccacgat gccaacgccc
     1441 agccccaggc gcacataggt cttgatgacg tcggagtcga tggcctccag cacgaagctg
     1501 ggcttgaggc gcctgtgctc gaaagccgcg tcgatgcgcg ggcggccggt aaaggaaggg
     1561 tggtaggaaa tgagcggctc ggcggcgagc tgatcgagcg tgagccgctc cacctgcgcc
     1621 agcgggtgct gggccggcat caccaccacg tgttgccact cgtagcaagg cagggtcacg
     1681 agctcgtcga agttcagcag cgattgggtg gccatgccga tttcggccga ttcgtcgagc
     1741 agcatgcgcg cgacctgctc cggcgtgccc tggtgcaggc tgaccgacac cttgggatag
     1801 cgcttgcgca gcgccgccac gggtgtcggc agtacgtagc gcgcctgggt gtgcgtggtg
     1861 gcgatggaca gcgtgccggc gtcctgcttg gagaactcct cgccgatgcg cttgaggttg
     1921 ccgacctcgc gcatgatgat ctcgatgcac ttgagcacct gctggccggg ctcggtgatg
     1981 cgcttgagcc gcttgccgtg gcgcgcgaag atgtcgacgc ccagctcctc ctcgagctcc
     2041 aggatggcct tggagatgcc gggctgcgag gtgtagagcg ccttggccgt ctcggtcagg
     2101 ttcaggttgc ggcgggcggc ctcctggacg aagcggaact ggtgcagatt catgggccta
     2161 agccttgctg ttgttcgttg ttgtcgtcgt cgtgctcagc gccaaggcgg cctcggccat
     2221 cgcggctatc acctgaggct gctcgccgat ggcgcccgcg agcgtccacc tgacctgcgg
     2281 gtgatccgcc tgcaacgaag ccagcagctc gggcaggtcc ttgcgcacat ggccgccggc
     2341 accgaggaag agcggaacga tgcagacgcg gcggcagccc gagcgcacga gctcttcggc
     2401 ggcggcccgc aattctggtt gcataaattc gaggaaagcg agcctgacct ccacatcggg
     2461 ggcgtgctcg cgcagacgcg tggcgacggc ctcgaagggg atcgcccagg ccgggtcgcg
     2521 cgcgccgtgc gcgaacagca gcacgcccgc ctggctcatc atcggtacct caccagccag
     2581 ctgaaggcgg ccagcgacag cagcagatag atcgcgctgg gcgcggccgc ggcgatccag
     2641 ggcgtccagt tctgcagcag gcccaggtgg ttggagatgt tgttgagcag cacgaagctg
     2701 atgcccagca tgatgccgcc gaagaccttg aagctcaggc cgccgctgcg cgaatgcaga
     2761 tacgcgaagg gcagggccag gctcatcatc acgaggcagg cgaagggata gagcgccttc
     2821 ttccagaact ggatctcgta gcgctgcgcg gcctgctcct gcgtggacaa atggctcatg
     2881 tagctgaaga gcgagaccgt cgtcatggtg ttcagcggca gcacggccgc cgagaccacg
     2941 cgcatcgtca ggccagtggg ccagtccatc tgggcgagcg cttccccgcg cggtgcgggc
     3001 tgggcctcgt ccagcttcca atgcgtgaca tccacgttct gcagctgcca gctcccggcg
     3061 ccgccgaccc ggccgctctg cgccgcgatg cgctggatca gtcggccgtc gggaccgaac
     3121 tcgaagatgc ggatgtcgtg gaattcgccg tcggcggtgg cgcgtccgac attgatcgag
     3181 aagttgtgct cgccgtcgct catgtggcgg ctgtccttga gccaggcgcc ggtgcgctcc
     3241 agcgagaggc cgcccttgaa gcgtgcctgc agcagcgtgg cctcgcgctc ggcggccggg
     3301 gcgatgaagt cgccgaccac gaaggtgaag atgccgaagc acagccccag cgaagccagc
     3361 aggctcaagg cgcgcccggg gcttagtccg cccgtgcgca ggatggtgta ttcggaggac
     3421 tgcgccagcc gggccattgc gtagatggcg ccgatcagga tcgcgatcgg cagcagctcg
     3481 tagatatggc cgggcagctc cagcaggcag tagagcaggg cgtggtagac cgtgtagccg
     3541 tggtcgccga cgttttccag cgcatcgacg atgtcgatga agaagaacag cgacagaaag
     3601 gcggcagcca cgaagaagac ggcgctcagc acgtcggtgt acagcaggcg tctgacggtg
     3661 cgcatggcgc tcagctcccc atgcgcagcc gcggcgcaaa tctcgccgag ccgctgtcgc
     3721 gccaccaaag cagcgccaag gcgagcacga aggtgccgcc gtgcaccagc gccaggcagg
     3781 cgccgaggct caatcggccc gaggcgaccc aggcctgcga caggttcagc aggttgtagt
     3841 agacgatgaa ggccatcagc gcatacagca ggttccagct gccgccgcgg cgcgggttgc
     3901 ctgcggacag gccgatgccc agcagcagca ggttgatgcc gcccatcgcc agccccaggc
     3961 gccacaccag ctcaccctgg gcccgcggcg agggctggct catcaggtcc agcgtcgagc
     4021 gcgcgcgcgg cagcagctcc tcggcgcgcg ccgagacgtt ctctcccacc a
//
LOCUS       DASUCY010000650         2176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_207495, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000650 DASUCY010000000
VERSION     DASUCY010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207495"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1192
                     /gene="amt"
                     /locus_tag="VFV25_12870"
     CDS             <1..1192
                     /gene="amt"
                     /locus_tag="VFV25_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEY1394258.1"
                     /translation="FVTFSMIVVLWVLYGYSLAFTEGNAFVGGFDRLFLKGVFDPATG
                     NFANAATFSKGVVLPEIVYVVFQATFAAITCCLIVGAFAERMKFSAVLLFTALWFTFS
                     YAPIAHMVWFWMGPDAYTGKEVVDAVNAKAGLIWQWGALDFAGGTVVHINSAVAGLVG
                     AFMVGKRVGYGKESFTPHSLTLTMVGAALLWVGWFGFNAGSALEANGSAALAFLNTFS
                     ATAVAVLAWCLGEALMKGKASMLGAASGAVAGLVAITPAAGNVGIVGALVVGAVAGFA
                     CLWGVNGLKKIHGADDSLDVFGVHGVGGIVGALLTGVLNTQVLGGPGQVTDWVTATVG
                     SNGIGAQFWIQLKAVLVTIVWSGVVSLIAYKIVDMTIGLRVTEDEEREGLDISSHGET
                     AYVK"
     gene            1402..>2176
                     /gene="gshA"
                     /locus_tag="VFV25_12875"
     CDS             1402..>2176
                     /gene="gshA"
                     /locus_tag="VFV25_12875"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEY1394259.1"
                     /translation="MVPHLITALTGPINELEQRIIESMPAIERWFRLEWMEHTPPFYT
                     SVDVRNAGFKLAPVDTNLYPGGFNNLTPEMLPLAVQAAMAAIEKICPEAKNLLLIPEK
                     HTRNTFYLTNVARLMQIFNQAGLNVRLGTLDENITEPTSLALPDGNSITVEPLMRSKR
                     RLGLKDFDPCTILLNNDLSAGIPKVLEDIHEQYLLPPLHAGWAVRRKSQHFAAYEEVA
                     KKFAKLLGMDPWLINPMFAQCGEVDFTEGGGLECLQSNVD"
BASE COUNT          357 a          721 c          699 g          399 t
ORIGIN      
        1 cttcgtgacc ttctcgatga tcgtcgtgct gtgggtcctc tatggctaca gcttggcctt
       61 caccgagggc aatgccttcg tcggcggctt cgaccggctg ttcctcaagg gcgtgttcga
      121 tcccgccacc ggcaacttcg ccaacgccgc gaccttcagc aagggcgtgg tgctgcccga
      181 gattgtctac gtggtgttcc aggccacctt cgcggccatc acctgctgcc tgatcgtggg
      241 cgccttcgcc gagcgcatga aattctcggc ggtgctgctc ttcacggcgc tgtggttcac
      301 cttcagctat gcaccgatcg cgcacatggt ctggttctgg atgggcccgg atgcctacac
      361 cggcaaggaa gtggtcgatg cagtgaacgc caaggccggc ctgatctggc agtggggcgc
      421 actggacttc gccggcggca ccgtggtgca catcaactcc gcggtggctg gcctggttgg
      481 cgccttcatg gtcggcaagc gcgtgggcta cggcaaggaa tccttcacgc cgcactcgct
      541 gacgctcacg atggtcggcg ccgccctgct gtgggtgggc tggttcggct tcaacgcggg
      601 ctctgccctg gaggccaacg gcagcgccgc gctcgccttc ctcaacacct tctccgccac
      661 tgcggtcgcc gtgctggcct ggtgcctggg tgaagcgctg atgaagggca aggcctcgat
      721 gctgggcgcc gcttcgggtg ccgtcgcagg cctggtggcg atcacgccgg cagccggcaa
      781 cgtcggcatc gtcggcgcgt tggtcgtcgg tgcggtcgcg ggcttcgcct gcctgtgggg
      841 cgtcaacggc ctgaagaaga tccacggcgc cgatgactcg ctggacgtgt tcggcgtgca
      901 cggcgtgggc ggcatcgtcg gcgcgctgct gaccggcgtg ctcaacaccc aggtgctggg
      961 cggtccgggc caggtgaccg actgggtcac ggccacggtc ggctccaacg gcatcggcgc
     1021 gcagttctgg atccagctca aggctgtgct ggtgaccatc gtgtggtccg gcgtcgtctc
     1081 gctgatcgcc tacaagatcg tcgacatgac gatcggcctg cgcgtgactg aagacgaaga
     1141 gcgcgagggc ctggacatca gctcgcacgg cgaaaccgcc tatgtgaagt gattcaagtc
     1201 cgaatctgat tgcgtggcac ggccagcccg tgcatttcaa gggccgctac catggcggcc
     1261 cttgtctttt tttgcgcggc gaaaaagggc acaacgggag ctgcgcggct ggcttgagtc
     1321 cggcgcgctc gccctcaaat ccctagaatt ctcgattcgc cgctgccccg agccggcgcc
     1381 ctcaccgcaa ggcatccatc catggttccc cacctgatca ccgcactcac cggccccatc
     1441 aacgaactcg agcagcgcat catcgagtcg atgccggcta tcgagcgctg gttccgactc
     1501 gaatggatgg agcacacgcc gcctttctat acctcggtgg acgtgcgcaa tgccggcttc
     1561 aagctggcgc cggtggacac caatctctac cccggcggct tcaacaacct cacgcccgag
     1621 atgctgccgc tggcggtgca ggccgcaatg gcggcgatcg agaagatctg ccccgaggcc
     1681 aagaacctgc tgctgattcc cgagaagcac acgcgcaaca ccttctacct gaccaatgtc
     1741 gcgcggctga tgcagatctt caaccaggcc ggcctcaacg tgaggctggg cacgctggac
     1801 gagaacatca ccgagccgac ctccctcgcg ctgcccgacg gcaacagcat cacggtcgag
     1861 ccgctgatgc gcagcaagcg ccgcctgggg ctcaaggatt tcgacccctg caccatcctg
     1921 ctcaacaacg acctgtcggc cggcattccc aaggtgctgg aggacatcca cgagcaatac
     1981 ctgctgccgc cgctgcacgc cggctgggcg gtgcggcgca agagccagca tttcgccgcc
     2041 tacgaggagg tggccaagaa gttcgccaag ctgctgggca tggacccctg gctgatcaac
     2101 ccgatgttcg cgcaatgcgg cgaggtcgac ttcaccgagg gcggcggact ggagtgtctg
     2161 cagagcaatg tcgatg
//
LOCUS       DASUCY010000651         3098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1058971, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000651 DASUCY010000000
VERSION     DASUCY010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1058971"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="VFV25_12880"
     CDS             <1..713
                     /locus_tag="VFV25_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1394260.1"
                     /translation="TRADAMLMEAEALARRKGFSHHAIAAGQGMLRFHEGRLDEAEEL
                     FRQARALCKSAGDHVNEYQANEFLVMLDLQRGALIQAREQCAELLALGDKLRGGSEEP
                     FARAMAGLCRLAIEDQADSLDAALSDLRLADAKHRLAYVLTRAALLDCERGRVARATE
                     RATEALSYATLLERATEMLIANAVLAHECAKSGDPAGAALHAREAERALAAGAAAWTR
                     SIVSRLAKPRRAARASST"
     gene            727..1254
                     /locus_tag="VFV25_12885"
     CDS             727..1254
                     /locus_tag="VFV25_12885"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-binding protein"
                     /protein_id="HEY1394261.1"
                     /translation="MTDILVERHWDQALSDADLQAALASAGECLGIHRVNWHSSRLSA
                     DGHELFCHFSAADAESVRIALSQSGSPRGQVWAGTVHDAPGVADADLARVNVLACRRF
                     DSPTSFEAADTPEQAGASCLLTHRVTRVRSYLSADRKRMVSLYQAPDAESVRLALQAA
                     GVAAERVWAFRQFRP"
     gene            1380..1952
                     /locus_tag="VFV25_12890"
     CDS             1380..1952
                     /locus_tag="VFV25_12890"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEY1394262.1"
                     /translation="MSNTTAIPAADNGVNVAALLGAREALGNAPQAAQFKWRASNEWI
                     KGTHSRSTVDGFYGLGGEQKHREARQFCTDHPEVFASEDRGITPTEMILVGLAGCLSA
                     GIASVAQHRNIQLHSVKATVEAGMDLQGILGIDTNVRNGFDGIQVHYEIDADATAEQI
                     AALVAQSQKRSAVFDIIANPTDISVTSRKA"
     gene            1963..>3098
                     /locus_tag="VFV25_12895"
     CDS             1963..>3098
                     /locus_tag="VFV25_12895"
                     /inference="COORDINATES: protein motif:HMM:NF025114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY1394263.1"
                     /translation="MSGTVWAEPAGSVDVVVIGAGHAGLAMSHTLGQRDIAHVVLERG
                     QVANSWRTERWDSLRLLTPNWMTRLPGCAYEGDDPDGYMSAAEVADFIAGYAASTGAP
                     VLTRTTVTRVAQRNGGHLVETDRGHWTCRAVVLATGACSRPVVPRMADAVPATVEQLT
                     PLQYRNPGQLRQGGVLVVGASATGLQLAQEIRRSGRPVTLAVGEHVRLPRMYRGRDIQ
                     WWMHAAGVLDQRIEDADDAERARRVPSPQLVGTPAHATMDLNALRSEGVELVGRLAGV
                     RDGRALFSGSLRNVCALADLKMNRLLDSIDGWAVQNSLDGCVGRVERFAPTDVGTSPR
                     LSLLFGQDVRTLIWATGFRPDYSWLDVPVFDPRGGLKHDRGVVAA"
BASE COUNT          504 a         1063 c         1080 g          451 t
ORIGIN      
        1 tgacacgcgc cgacgccatg ctgatggagg ccgaggcact ggcccgacgc aagggcttca
       61 gccaccacgc catcgcggcc ggccagggca tgctgcgatt tcacgaaggc cggctggacg
      121 aggccgagga gctttttcgc caggcgcggg ccctgtgcaa gtcggcgggc gaccacgtca
      181 acgagtacca ggccaacgag ttcctggtca tgctggacct ccagcgcggc gcactcatcc
      241 aggcccgcga gcagtgcgcc gaactgctgg cgctgggcga caagctgcgc ggcggcagtg
      301 aagaaccctt tgcccgcgcc atggccgggc tgtgtcgcct ggccatcgag gaccaagccg
      361 actcattgga tgccgccttg tccgatctgc gactggccga cgccaagcac cgactggcct
      421 acgtattgac acgcgcggcg ttgctggact gcgaacgcgg ccgcgtggcc cgtgccaccg
      481 agcgggccac agaggcgctg agctacgcca cgctgctgga acgcgccacc gagatgctca
      541 tcgccaatgc cgtgctggcg cacgaatgtg caaagtccgg cgaccccgcc ggcgcggccc
      601 tgcacgcacg agaagcagag cgggccctcg cggcgggtgc cgcggcttgg acgcgtagca
      661 tcgtgtccag attggccaag ccccggcgcg ccgcacgcgc gtcatccacc tgagtaagag
      721 cagaccatga cagacatcct ggtcgaacgc cactgggacc aggcgctgag cgacgccgac
      781 ctgcaggccg cactggccag cgccggtgag tgcctgggca tccatcgcgt caactggcat
      841 agcagcaggc tgtccgccga cggccatgag ctcttctgcc acttctcggc ggcggatgcc
      901 gagtcggtgc gcatcgcact gagccagtcc ggctccccgc ggggccaggt gtgggcgggc
      961 accgtgcacg acgcgccggg tgtcgccgat gcggacctgg cccgggtcaa cgtgctggcc
     1021 tgccggcgct tcgactcgcc gacgtccttc gaggccgccg atacgcctga gcaggcgggc
     1081 gcaagctgcc tgctgaccca ccgtgtgacg cgtgtgcgca gctacctgtc ggccgatcgc
     1141 aagcgcatgg tgagcctgta tcaggcgccg gatgccgaat cagttcggct ggcgctgcag
     1201 gcggcgggcg tggcggcgga gcgggtctgg gcgttccggc agttcaggcc atgagcccag
     1261 cgcgctcgaa ggcgagataa cacgctggct accacgctgg gttccacgcg cggcgcacac
     1321 agtcactcct gtccggcgat cgatccgggc tcgttcatca accgacagga gcattgacca
     1381 tgagcaacac caccgcaatc ccggccgccg acaacggcgt caacgtcgcc gccctgctgg
     1441 gcgcccggga ggccctgggc aacgcgccgc aagccgccca gttcaaatgg cgcgccagca
     1501 acgagtggat caagggcacg cacagccgca gcaccgtgga tggcttctac ggcctgggag
     1561 gcgagcagaa gcatcgcgag gcgcgccagt tctgcaccga ccaccccgaa gtcttcgcct
     1621 ccgaggacag gggcatcacg cccacggaga tgatcctcgt gggcctggcc ggctgcctga
     1681 gcgccggcat cgcctcggtc gcccagcacc gcaacatcca gctgcactcg gtcaaggcca
     1741 ccgtcgaggc gggcatggat ctgcagggca tcctgggcat tgacaccaac gtgcgcaacg
     1801 gcttcgacgg catccaggtg cactacgaga tcgacgccga cgccacggcc gagcagatcg
     1861 cggcgctggt ggcacagtcg cagaagcgat cggcggtgtt cgatatcatc gccaacccga
     1921 ccgacatcag cgtcaccagc cgcaaggcct gagaggtccg gcgtgagcgg gacagtctgg
     1981 gccgagcccg cgggcagcgt ggacgtggtc gtcatcggcg ccggccatgc cggcctggcc
     2041 atgagccata cgctgggaca acgcgacatc gcccatgtgg tgctggagcg cggccaggtg
     2101 gccaacagct ggcgcaccga acgctgggat tcgctgcgcc tgctcactcc caactggatg
     2161 acgcggctgc ccggctgcgc ctatgaaggt gacgaccctg acggctacat gagtgccgcc
     2221 gaggtggccg acttcatcgc gggctacgcc gcatcgaccg gtgcgccggt gttgacccgc
     2281 accacggtga cgcgcgtggc tcagcgcaac ggcggccatc tcgtggagac cgaccgcggc
     2341 cactggacct gccgcgccgt ggtgctggcc actggcgcct gcagccggcc cgtggtgcca
     2401 cgcatggccg acgctgtgcc ggcgaccgtc gaacaactga cgccgctgca gtaccgcaac
     2461 cccgggcagt tgcgccaggg cggcgtgctg gtggtcggtg cctccgccac gggcctgcag
     2521 ctggcgcagg agatccgccg cagtggccgc cccgtcacgt tggccgtcgg cgagcatgtg
     2581 cgtctgccgc gcatgtatcg cggccgggac atccaatggt ggatgcacgc cgcgggtgtg
     2641 ctggaccagc gtatcgaaga cgcggacgat gccgagcggg cgcgccgcgt gccctcgcca
     2701 cagctggtgg gaacccccgc gcacgccacg atggacttga acgcgctgcg cagcgagggc
     2761 gtggaactgg tcggccgcct ggcgggggtg cgcgatggac gggccttatt ttcaggttca
     2821 ctgcgcaacg tctgtgcgct ggcggacctg aagatgaacc gcttgctcga cagcatagac
     2881 ggctgggcgg tgcagaactc gctggacgga tgtgtggggc gcgtcgaacg ctttgcgccg
     2941 accgacgtcg gcacgagccc ccggctctcg ttgttgttcg gccaggatgt ccgcacgttg
     3001 atctgggcca ccggatttcg tccagactac agttggctcg acgtgccggt gttcgacccg
     3061 cgtggtgggc tcaaacatga ccgtggtgtc gtggcagc
//
LOCUS       DASUCY010000652         5731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1460527, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000652 DASUCY010000000
VERSION     DASUCY010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 5731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5731
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1460527"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="VFV25_12900"
     CDS             complement(<1..667)
                     /locus_tag="VFV25_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1394264.1"
                     /translation="MDTNNSSARTRWQRFSTTAVNAYHAYGSWLVSISWKRFFVLSML
                     LMIIAAVLQDLPPFSWRFTETVDLVPSVSPPSPPKPPSVKLDRKPTISFDKPASGGDK
                     GGVDITIDENGVRIKHRSREGAAAASGAASATAPAASQASAAASDEGLQIHLPPEIAN
                     NPDVKDAIEEARKAVADAAADAENTRREAEDAKHEAEAIRIEAEKQASRALKRVHTWS
                     VGDM"
     gene            complement(846..1382)
                     /locus_tag="VFV25_12905"
     CDS             complement(846..1382)
                     /locus_tag="VFV25_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEY1394265.1"
                     /translation="MLARLTLNTLALAVGAAFLAGSALAQQTAAPTAQHHHHQAAAST
                     IEVEAAWARPTVEGQQVGGGFLTLKNTGPGADKLIGGSTPVAERLELHMMRMDGNVMR
                     MDEVPAIELPAGQSVEFKPGGYHLMFRGLKAPLKLGSSVPVTLKFEKAGELKLQMKVL
                     RAPAAHMHEDAGQQHEHH"
     gene            complement(1507..2439)
                     /locus_tag="VFV25_12910"
     CDS             complement(1507..2439)
                     /locus_tag="VFV25_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEY1394266.1"
                     /translation="MSSPALTHLALRGLAVFERGWLSSNNVLMFDDDGGATLIDSGHC
                     VHAKQTLALVRHALAGRALTRVVNTHLHSDHCGGNAALERAFGVHAHIPPGLWQAVQR
                     WDEDALSYRATGQRCERFTPAGRIVPGEPLCAGGRRWEVIAAPGHDPHSVILFDAAQG
                     VLISADALWENGFGVIFPELEGASGFDEAAAVLDALAGLPVRVVIPGHGAPFEDVAGA
                     LARARARLAALRTDPRKHARHAAKVLLKYHLMEEHSQPLEGVLAWAEATPLFATIWAS
                     LAPAQPLREGALLLLRELADSGALLLDEGLVSDR"
     gene            complement(2436..3035)
                     /locus_tag="VFV25_12915"
     CDS             complement(2436..3035)
                     /locus_tag="VFV25_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY1394267.1"
                     /translation="MIWHMPRTLSCCTALLLAALAPAVRAEAVSYGLDPEHTYVNWEV
                     LHLGTSMQRGRFDKLTGNVSFDAKARQLEIAITVDTSSISSGSASFDRILRGGELLDV
                     AQFPQAWFVASHARFDGETPSEIRGEITLRGKSQPLALHALRWRCGLNPVFKREVCGG
                     DFEATLKRSDFGMTLVLPLVADEVKLLIEVEGIRGGLPQ"
     gene            3162..4937
                     /locus_tag="VFV25_12920"
     CDS             3162..4937
                     /locus_tag="VFV25_12920"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEY1394268.1"
                     /translation="MAERLLAAQARLLHERTSGIVGAGLASSLLVVWLLWSHDAHAGL
                     LAWLGLRLAMIVLLARQRSRYGREPDRRWRRRLTAITTLDGALWSVLVVVFSPAPQDP
                     IALILLATMVGVAAVAMVAMAVHAPLHIAYSTAVLAPCALWQLLRLDAAGAYIGLSLL
                     LYLAFIVHEGRISGRHLKEMLSLRLRLEDEAQARAQALALAERHSAVKSQFLATMSHE
                     MRTPLHGILGLTRELMRKPQPDPAGQRDAHRQLALIEQSGQHLLELISDLLDFSRIEA
                     GQMKMAKRPFDLAAVVSEVAALATAGARDKGLSFAVEMAGGEAGWRAGEAHWVLGDAS
                     RVRQVLLNLAGNAVKFTDRGGVKLKVWPDTGDGLVHIDVQDSGIGIAPAELAHIFDAF
                     HQVESSFGRRYGGTGLGLTISRELARAMGGELSCISRAGQGSTFSFSAHLPRCAAPEL
                     APPGPPHVTPLRGRVLLAEDNEVNALVAEAVLKQAGLEFETVGDGAAAVHRCIQSDAA
                     LPAIDLVLMDCHMPTLDGFAATRLIRQHEAAVQGPRLPIIALTANAYDSDRERCLIAG
                     MDAHLPKPFRAEELRRLLETYLPRA"
     gene            complement(4945..>5731)
                     /locus_tag="VFV25_12925"
     CDS             complement(4945..>5731)
                     /locus_tag="VFV25_12925"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEY1394269.1"
                     /translation="RSGIAIDPPGGFFFWMPAHGDEALGLKIVSFYPPNAGRGLPTHT
                     ATIFLLDPQTGAPLAVMDGRLITEMRTAAVSAVATRLLARPDAQVLALLGSGVQARSH
                     YEALKLVRPFTQIRVWSRTPEHARRFADEIGAIATTAEDAVRGADVVVTATSAVEPVL
                     QGAWLKPGCHVNAVGAPRPNWRELDDAAMSNLLFVDSRAGALKESGDVILSKAPIYAE
                     IGEALAGKLPHRSDETTIFKTLGMAVEDLAAALLVYRKVSRAA"
BASE COUNT          840 a         1930 c         2019 g          942 t
ORIGIN      
        1 gcatgtcgcc cacgctccag gtatgcaccc gcttgagcgc ccgcgaggcc tgcttctcgg
       61 cttcgatacg gatggcttcg gcttcgtgct tggcgtcttc ggcttcgcgc cgggtgttct
      121 cggcgtctgc cgctgcatcg gccacggcct tgcgcgcctc ttcgatcgcg tccttcacgt
      181 cggggttgtt ggcgatctcg gggggcagat ggatctgcag accctcgtcg ctggcggcgg
      241 ccgaggcctg ggacgcggcg ggcgcggtcg ccgatgcggc gcccgaggcc gcagccgcac
      301 cttcccttga gcggtgcttg atgcgcacgc cgttttcgtc gatggtgatg tccacgccgc
      361 ccttgtcgcc gcccgaggcc ggcttgtcga acgagatcgt gggcttgcgg tcgagcttca
      421 ccgacggcgg tttgggcggg ctcggcgggc tgacgctggg cacaaggtcc actgtctcgg
      481 tgaagcgcca gctgaagggc ggcagatcct gcaggacggc cgctatgatc atcagcagca
      541 tcgagagcac gaagaagcgc ttccagctga tgctgaccag ccagctgccg taggcgtgat
      601 acgcgttgac cgccgtggtc gagaagcgct gccagcgggt acgcgcagag ctgttgttgg
      661 tatccatggc gccactgtaa gcggcgcccc gccggccccg aagcgccgcg cgatgcgctg
      721 tcgtcggctc atgacaggct gcagcgccgg gcgacggagc gcgggctggc tgccggcagg
      781 gattcaggcc ccgggcagtc cgatgaggaa aagttcacgc ccgggccttg aaacccggcg
      841 ctgaatcagt ggtgctcgtg ctgctggccg gcatcctcgt gcatgtgtgc ggccggcgcg
      901 cgcaacacct tcatctgcag cttcagctcg ccggccttct cgaacttgag cgtcacgggc
      961 acgctgctgc cgagcttgag cggagccttc aggccgcgga acatcaggtg gtagccgccg
     1021 ggcttgaact ccacgctctg gccggccggc agctcgatgg ccggcacctc gtccatgcgc
     1081 atcacgttgc cgtccatgcg catcatgtgc agttccagcc gctcggccac tggcgtgctg
     1141 ccgccgatca gcttgtcggc gccggggccg gtgttcttca gtgtcaggaa gccgccgccc
     1201 acctgctggc cctcgacggt cgggcgggcc caggccgcct cgacctcgat cgtgctagcc
     1261 gccgcttgat gatggtgatg ttgagcggtg ggcgcagctg tttgctgagc gagggccgat
     1321 ccggccagga acgccgctcc aacggccagg gcgagggtat tgagcgtcag gcgagcaagc
     1381 atggggcgaa ctccggggaa tgcgatgtga cgaagccgag gctcggcgcc tccgggcggg
     1441 ttggatggca tgcgggcgcc ggtggttcga tgacagcggc gcgccgcgca agccaacgat
     1501 tgtgcttcag cggtcgctga ccaggccctc atccaggaga agcgcgccgc tgtcggccaa
     1561 ttcccgcagc aggagcaggg ctccctcgcg cagcggctgc gcgggcgcca ggctggccca
     1621 gatggtggcg aagagcggcg tcgcctcggc ccaggcgaga actccttcaa gcggctggct
     1681 gtgctcttcc atcaggtggt acttgagcag caccttggct gcgtggcggg cgtgcttgcg
     1741 agggtcggtg cgaagcgcgg ccaggcgggc gcgggcgcgc gccagtgcgc cggccacgtc
     1801 ttcgaaggga gcgccatggc cggggatcac gacccgcacc ggcagcccgg cgagcgcatc
     1861 gagcacggcg gctgcttcat cgaagccgga cgctccttcc agctccggaa agatcacgcc
     1921 gaagccgttc tcccacagcg cgtcggccga gatcagcacc ccctgcgccg cgtcgaagag
     1981 gatcaccgaa tgcgggtcgt ggccgggcgc ggcgatcact tcccagcgcc gcccacccgc
     2041 gcacagaggc tcgcccggca cgatgcggcc ggccggcgtg aagcgctcgc agcgctggcc
     2101 ggtggcgcgg taactgagcg cgtcctcgtc ccaacgctgc accgcctgcc acagccccgg
     2161 cgggatgtgg gcgtgcacgc cgaaggcgcg ctccagcgcc gcattgccgc cgcagtggtc
     2221 ggaatgcaga tgcgtgttga cgacgcgcgt cagcgcgcgc ccggccaatg catgacgcac
     2281 gagcgcgagc gtctgctttg catgcacgca atgtccgctg tcgatcagcg tggcgccgcc
     2341 gtcatcgtcg aacatcagca cgttgttgga cgacagccag ccccgctcga acaccgccag
     2401 gcctcgcagg gccaggtggg tcagagcagg gctgctcact gcggcaagcc cccgcgtatg
     2461 ccctctacct cgatcagcag tttgacctcg tcggccacca gcggcagcac cagcgtcatg
     2521 ccgaagtcgc tgcgtttgag cgtggcctcg aagtcgccgc cacagacctc gcgcttgaac
     2581 acaggattga gcccgcagcg ccagcgcagg gcgtgcagcg cgagcggctg gctcttgccc
     2641 cgcagcgtga tctcgccccg gatctcgctc ggcgtctcgc cgtcgaagcg ggcatgggac
     2701 gcgacgaacc aggcctgcgg aaattgagcc acgtcaagca gctcgccgcc gcgaagtatg
     2761 cggtcgaaac tcgccgagcc cgagctgatc gagctggtgt ccaccgtgat ggcgatctcg
     2821 agctgccgag ccttggcatc gaagctgaca ttgcccgtca gcttgtcgaa gcgaccgcgc
     2881 tgcatcgagg ttcccaagtg caggacttcc cagttcacat aggtgtgctc ggggtccaag
     2941 ccatagctca cggcttcggc acgcacggcc ggggccagtg cggccagcag cagcgccgtg
     3001 cagcaggaca gggtgcgggg catgtgccag atcatcggcg cattctgcag tgggcattag
     3061 agtcaggggt attccgtgta ttcgaccctt gcgcgcccgc tgggctgctg ttggaatgcc
     3121 tgcgggagga gtatcggttg agcgccgagg tatctgtttc catggctgag cgcctgctgg
     3181 cggcccaagc gaggctgctg cacgagcgca ccagcggcat cgtcggggcg ggcctggctt
     3241 cctccctgct cgtggtctgg ctgctgtggt cccacgatgc ccatgccggg ctgctggcct
     3301 ggctggggct gaggctggcc atgatcgtgc tgctggcccg gcaacgctcg cgctatggcc
     3361 gcgaaccgga tcggcgttgg cgtcgcaggt tgaccgccat caccacgctg gacggcgcgc
     3421 tgtggagcgt gctggtcgtc gtcttctctc cggcgccgca agacccgatc gcgctgatcc
     3481 tgctggccac catggtgggc gtggcggccg tggccatggt ggcgatggct gtccatgcgc
     3541 cgctgcacat cgcctacagc acggctgtgc tggcgccttg cgccctgtgg cagctgctga
     3601 ggctggacgc ggccggcgcc tatatcggcc tgagcctgct gctctacctc gccttcatcg
     3661 tccacgaggg caggatctcg gggcggcatc tgaaggagat gctgagcctg cgcctgcgct
     3721 tggaagatga ggcccaggcg cgcgcccagg cactggcgct ggccgagcgg cacagcgcgg
     3781 ttaagagcca gttcctcgcc accatgagcc acgagatgcg cacgcccttg cacggaatcc
     3841 tggggctgac acgcgagctg atgcgcaaac cgcagccgga ccccgcaggc cagcgtgatg
     3901 cgcaccggca gctggcgctg atcgagcagt cgggccagca tctgctcgag ctgatcagcg
     3961 acctgctcga cttctcgagg atcgaggccg gccagatgaa gatggcgaag cggcctttcg
     4021 acctggccgc ggtggtgtcc gaggtggcgg cgcttgcgac cgcaggcgcg cgcgacaagg
     4081 gcttgagttt cgcagtggag atggcaggcg gcgaggctgg ctggcgcgcc ggcgaggcgc
     4141 attgggtgct gggcgatgcc tcgcgcgtgc gccaggtact gctcaacctg gccggcaatg
     4201 ccgtcaagtt caccgaccgc ggcggcgtca agctcaaggt ctggccggac accggcgacg
     4261 gcctggtgca catcgacgtg caggattcgg gcataggcat cgcgcctgcc gagctggcgc
     4321 atatcttcga tgccttccac caggtcgaga gcagcttcgg gcgccgctac ggcggcaccg
     4381 gcctgggtct cacgatttcg cgcgagctgg cgcgcgccat gggcggcgag ctcagctgca
     4441 tcagtcgagc gggccagggc tcgaccttca gcttcagcgc gcacttgccg cgctgcgcgg
     4501 cgcctgagct tgcgccaccg ggacccccgc acgtgacccc tttgcgcggc cgcgtgctgc
     4561 tggcggaaga caacgaagtg aacgctctgg tcgccgaggc cgtgctcaag caggcggggc
     4621 tggagttcga gaccgtgggc gacggtgcgg ccgccgtgca tcgctgtatt cagtctgacg
     4681 ccgcgctgcc ggcgatcgac ctcgtgctga tggactgcca catgccgacc ctggacggct
     4741 tcgccgccac gcgcctgatc cgccagcacg aggcggcggt gcaggggccg cgcctgccca
     4801 tcatcgcgtt gaccgccaat gcctatgaca gcgatcgcga gcgctgcctg atcgccggca
     4861 tggacgccca tttgcccaag ccctttcgcg ccgaggaact gcgccggctg ctcgagacct
     4921 atctgccgcg tgcctgagcc tgcttcaagc ggcccttgac actttgcgat agaccagcag
     4981 ggcggcggcc agatcctcga cggccatgcc cagcgtcttg aagatcgtcg tctcgtccga
     5041 cctgtgcggc agcttgcccg ccagcgcctc gccgatctcc gcgtagatcg gggccttcga
     5101 gaggatgacg tcgcccgatt ccttcaacgc gcccgcgcgg gaatcgacga agagcaggtt
     5161 ggacatcgcc gcgtcgtcga gttcgcgcca gttcggccga ggggcgccca cggcgttgac
     5221 atggcagccg ggcttgagcc atgcgccctg gagcacgggc tcgacggcgc tggtcgcggt
     5281 gacgacgaca tcggcccccc gcaccgcatc ctccgcggtg gtcgctatgg ccccgatctc
     5341 atcggcgaag cggcgcgcat gctcgggcgt gcggctccag acgcgaatct gcgtgaacgg
     5401 cctgacgagc ttcagggcct cgtagtggct gcgtgcctgg acgccgctgc cgagtagtgc
     5461 cagcacctgg gcatcgggcc gcgccagcag cctggtcgca acggcggaca ccgcggccgt
     5521 gcgcatctcg gtgatcaggc gcccgtccat caccgcgagc ggagcgcccg tctgcgggtc
     5581 gagcaggaag atggtcgccg tgtgcgtcgg cagtccgcgc ccggcattgg gcggatagaa
     5641 gctgacgatt ttcagcccga gcgcctcgtc gccatgcgcg ggcatccaga agaagaatcc
     5701 gccgggtgga tcgatcgcaa tccctgatcg g
//
LOCUS       DASUCY010000653         3427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1267858, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000653 DASUCY010000000
VERSION     DASUCY010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1267858"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /gene="dusA"
                     /locus_tag="VFV25_12930"
     CDS             complement(<1..524)
                     /gene="dusA"
                     /locus_tag="VFV25_12930"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEY1394270.1"
                     /translation="MSESLRARHDPWRLSVAPMMDWTDRHCRYFHRQITQRTRLYTEM
                     VTTGALLHGDQPRHLDFDAAEHPLALQLGGSEPVDLAACAKLAARWGYDEINLNCGCP
                     SERVQRGAFGACLMAEPALVADCLKAMRDAVDILVTVKHRVGIDKGESYEFVRDFVGT
                     LAAKAGCEVFIVHAR"
     gene            complement(517..756)
                     /locus_tag="VFV25_12935"
     CDS             complement(517..756)
                     /locus_tag="VFV25_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1394271.1"
                     /translation="MQSPAKYLVIIEAGGPMVARLFDAKRSQVAEFDAASEEVAVMTA
                     GLQPVLGADAKTWSLALAGHNEAERAAALVYTLDV"
     gene            complement(814..1926)
                     /locus_tag="VFV25_12940"
     CDS             complement(814..1926)
                     /locus_tag="VFV25_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEY1394272.1"
                     /translation="MKPLPQLDHAGHVQAEVRAGLGLITLNRANALNALSLGMIRDLT
                     AILLHWADDAAVQAVVIRGAGREGKPPAFCAGGDIRFFHQAALDGDPRIEDFFTEEYR
                     LNHLIHGYAKPYIALMDGIVMGGGMGITQGAALRVVTEHSKLAMPETHIGLFPDVGGG
                     YFLSRCPGHVGEYLALAGHVVGAGDAIALGLADGYLSSSELPALVEALTALPPGQASE
                     YLAAAKSRLQAAPAPKLPAHREQIDRHFGAADMQAIVASLAADDSAWAGETLAALRKR
                     SPLMLAVTLEQIRRARHMTLADDLRMERDLVRHCFALRPGRESETVEGVRALAVDKDH
                     APRWNPPRIEDVTPEQVAEFFASPWQAHAHPLRDLR"
     gene            complement(1960..2826)
                     /locus_tag="VFV25_12945"
     CDS             complement(1960..2826)
                     /locus_tag="VFV25_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEY1394273.1"
                     /translation="MIIVTLMWSTAGVVTRHLDAARSFEVTFWRSAFNALALLVALSA
                     MRGAALWRGMAAAPWSLWVSGVCWAVMYTAFMVALTLTTVANVLITMAIGPLLTALFA
                     RLFLGTRLAARTWVAIAIAGLGIAWMFGKEIGGAGDHQVLGTLVALCIPLAAATNWTL
                     LQHVGQRASRRGEAVQDMLPAVLIGAALSALVSLPLAWPLQASGHDLGLLAMLGVFQL
                     AIPCLLVVRLTQVLPAAEISLLGLLEVIFGVLWAWLGAGEQPGATALSGGALVIGALV
                     GNELLALRKQRY"
     gene            complement(2979..>3427)
                     /locus_tag="VFV25_12950"
     CDS             complement(2979..>3427)
                     /locus_tag="VFV25_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEY1394274.1"
                     /translation="NLTRGSGLISNIFDSYGPHKGEIGSRKNGVLISMDDGEIFTYAL
                     FNLQERGRLMAAHGDEVYEGQIIGIHSRDNDLVVNATRTKQLTNFRVSGKEDAIKITP
                     PIELTLEYAVEFIEDDELVEITPKSIRLRKRYLKEHERKRAQREAA"
BASE COUNT          585 a         1190 c         1121 g          531 t
ORIGIN      
        1 cgcgcatgca cgatgaagac ttcgcagccg gctttcgccg ccagcgtgcc gacgaaatcg
       61 cgcacgaact cgtagctctc gcccttgtcg atgccgacgc ggtgcttcac cgtcaccaga
      121 atgtccacgg cgtcgcgcat agccttcagg caatcggcca ccagcgccgg ctcggccatc
      181 aggcaggcac cgaaagcgcc gcgctgcacg cgctcgctgg ggcagccgca gttgaggttg
      241 atctcgtcat agccccagcg cgccgcgagc ttcgcgcagg cggccagatc gaccggctcg
      301 ctgccgccca gctgcagcgc cagcggatgc tcggcggcgt cgaaatccag atggcgcggc
      361 tgatcgccat gcagcagcgc gcccgtggtc accatctcgg tgtagagccg cgtgcgctgc
      421 gtgatctggc gatggaagta gcggcaatgg cggtcggtcc agtccatcat cggcgccacg
      481 ctgaggcgcc aagggtcgtg ccgcgctcgc agggattcag acatccagcg tgtagaccag
      541 tgccgccgcg cgctcggcct cgttgtggcc agccagggcc aggctccaag tcttggcgtc
      601 cgcgcccagc acgggctgca ggccggcggt catcaccgcg acctcctccg aggcggcgtc
      661 gaactcggca acctggctgc gcttggcatc gaagagtctt gccaccatgg ggccgcccgc
      721 ctcgatgatg accaggtatt tcgcggggct ttgcatgatc ggtcgtcgtt tttctgctga
      781 tccggcctca gtctaatccc gcgcgctgcc tgctcagcgc aggtcccgca gcggatgggc
      841 atgggcctgc caggggctgg cgaagaactc ggccacctgc tcgggcgtca catcttcgat
      901 gcgcggcggg ttccaccgcg gcgcatggtc cttgtcgacg gcgagcgccc gtacaccctc
      961 caccgtctca ctctcgcggc cgggccgcag cgcaaagcag tggcgcacca gatcgcgctc
     1021 catgcgcagg tcgtcggcca gcgtcatgtg ccgcgcacgc cggatctgtt ccaaggtgac
     1081 ggccagcatc agcggcgagc gcttgcgcaa ggcggccagc gtctcgccag cccaggcgct
     1141 gtcgtcggcg gccagcgagg cgacgatcgc ctgcatgtcg gccgcgccga aatggcggtc
     1201 aatctgctcg cgatgtgccg gcagcttggg tgccggcgcg gcctgcagac gggacttggc
     1261 ggccgccagg tattcgctgg cctggccagg cggcagcgcc gtcagcgcct cgaccagggc
     1321 gggcagttcc gagctggaaa gatagccgtc ggccagaccg agggcaatcg catcgcctgc
     1381 gccgacgaca tgcccggcca aggccaggta ttcgcccaca tggccggggc aacgcgacag
     1441 gaagtagcca ccacccacgt cggggaagag gccaatatgg gtctcgggca tcgccagctt
     1501 gctgtgctcg gtgaccacgc gcagcgccgc gccctgagtg atacccatgc cgccacccat
     1561 cacaatgccg tccatcagcg cgatataggg cttggcgtag ccgtggatca ggtgattgag
     1621 gcggtattcc tcggtgaaga agtcctcgat gcgagggtcg ccgtccagcg ccgcctggtg
     1681 aaagaagcgg atgtcgccgc cggcgcagaa ggcgggcggc ttgccttcac ggcccgcgcc
     1741 acggatcacc acggcctgca ccgcagcatc gtccgcccaa tgcagcagga tggcagtgag
     1801 gtcgcggatc atgcccagcg acagcgcatt gagggcattc gcgcgattca aggtgatgag
     1861 gccgagcccc gcgcgaactt cggcctgcac atggcctgca tggtcgagct gaggcagagg
     1921 cttcatgacg ccggttcctt cttttggatt ccagagactt caatagcgct gcttgcgcag
     1981 cgccagcaat tcattgccca ccagcgcgcc tatgaccaga gcgccgccgc tgagggctgt
     2041 tgcgccgggc tgttcgccgg cacccagcca ggcccagagg acgccgaaga tcacctccag
     2101 caggcccagc agcgagattt cggcggcggg cagcacctgg gtgagcctca ccaccagcag
     2161 gcagggtatg gccagctgga acacgcccag catggcgagc aggccgagat cgtggcccga
     2221 ggcctgcagc ggccaggcca gcggcaggct cacgagggcc gacaaggcgg cgccgatcag
     2281 caccgccggc agcatgtcct gcaccgcctc gccgcgccgg ctcgcacgct ggccaacgtg
     2341 ctgcagcagc gtccagttgg tcgccgcagc caggggtatg cacaaggcca ccagcgtgcc
     2401 cagcacctgg tgatccccgg cgccgccgat ttccttgccg aacatccagg caatgcccag
     2461 gccggcgatc gcgatcgcga cccaggtgcg cgccgccagc ctggtgccga gaaagaggcg
     2521 cgcaaacagc gccgtcaaca gcgggccgat cgccatggtg atcagcacat tggcgacggt
     2581 ggtcagcgtg agcgcgacca tgaaggcggt gtacatcacc gcccagcaca cacccgaaac
     2641 ccagagcgac cacggcgccg cggccatgcc gcgccacagc gccgcacccc gcatggccga
     2701 cagcgcgacc aacagcgcca aggcattgaa ggcactgcgc cagaacgtga cctcgaagct
     2761 gcgcgccgca tcgagatgcc gcgtcaccac gcccgccgtg ctccacatca gcgtgacgat
     2821 gatcatcccg acaactgcgc ggcgatgact caagcctcca gacattccct ccccagaggg
     2881 ccgcaaagcg gcccgggcgt gattcaagac agagcccccg ccaggcgggg gctgggggtg
     2941 cagtcagttc accgcgaagc ggtacctgcg atcaccactc aagcagcctc gcgctgagca
     3001 cgcttcctct cgtgctcctt caggtaccgc ttgcgcagcc ggatcgactt cggcgtgatc
     3061 tcgaccagct cgtcgtcttc gatgaactcg accgcgtatt cgagtgtcag ctcaatcggc
     3121 ggcgtgatct tgatcgcgtc ttccttgcca gacacccgga agttggtgag ctgcttggtg
     3181 cgcgtggcgt tgaccaccag gtcgttgtcg cggctgtgaa tgccgatgat ctggccctca
     3241 tagacctcat cgccgtgcgc cgccatgagc cggccgcgtt cctgcaggtt gaacagcgcg
     3301 taggtgaaga tctcgccgtc gtccatggag atcagcacgc cgttcttgcg gctgccgatc
     3361 tcgcccttgt gcggcccgta gctgtcgaag atgttcgaga tcaggcccga gccgcgcgtc
     3421 aggttca
//
LOCUS       DASUCY010000654         2550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_207603, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000654 DASUCY010000000
VERSION     DASUCY010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207603"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..934
                     /locus_tag="VFV25_12955"
     CDS             161..934
                     /locus_tag="VFV25_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEY1394275.1"
                     /translation="MKLGELTIPDRRSEVSAEEWQVRVDLAAAYRLVAEFKWDDLVFT
                     HITARVPGTHDQFLINPYGFLFEEITASSLVKIDLQGNKLDDSPFPVNPAGFTIHSAI
                     HGARHDAGCVLHTHTLNGVAVSAQKGGVLPLSQQSIFVLASLAYHDYEGVALREDEKA
                     RLLRDLGDKQHLMLRNHGLLTLGATVADAFLAMYLFETVCTIQVRAQSGGGELIAIDP
                     RIIATAREQAAQVTKGLGSQLTWPGLLRRLDRKDASYRS"
     gene            complement(922..1635)
                     /locus_tag="VFV25_12960"
     CDS             complement(922..1635)
                     /locus_tag="VFV25_12960"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEY1394276.1"
                     /translation="MTPRRRIYLMRHGSVDYFLADGTPVAPEGVVLNPEGRRQADAAG
                     ALFAASGIRFDRVVASGLPRTVETARRVLAAMGSSAPSMQIEPALQEIRSGRLADIPR
                     DQIEQAFLGVFRAGPDIEEHRFLGGESIGEMLDRALPAYERWLADPDWHCLLMVLHGG
                     INRALLSAALAGQRAFFGRIEQAPACINVLEVGHGGNSEAVVRAINLAPTQWLHERER
                     HTTMEKLLLQYRRSGAQDR"
     gene            complement(1660..2439)
                     /locus_tag="VFV25_12965"
     CDS             complement(1660..2439)
                     /locus_tag="VFV25_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEY1394277.1"
                     /translation="MPAELRTERREEALILTISDPATRNTLSPQVYDAGVEALGVAEA
                     NADVRCVIITGEGGHFSAGGNLQRLAANREAGRETQRESVARLNQLVEAIRAFPKPVI
                     AAVEGAAAGAGFSIALACDLLVAAEDARFVMAYNRIGLSPDGGSTWHLVQNLPRALAL
                     QLIWLAEPMTARELQAHGLVNRVTDKGQALNEALALARQLAGFAPNALASGKELVNLC
                     PSRDLAAQLDAERDHFVDNLFHDNAAEGLRAFFEKRPPRFR"
BASE COUNT          410 a          847 c          882 g          411 t
ORIGIN      
        1 ggcgggggag agcttggaga accacgagcc ggattcgatg ttggagcgtt cgtcgattgt
       61 aagaacgggg ttcatcgtct tgagtctctg acgtgacagc atgctgcgct gcggggaaac
      121 tattgtccat ggggcctaca cccgaatcct ggagcggacc atgaagcttg gtgaattgac
      181 catccccgac cggcgcagcg aggtcagcgc cgaagaatgg caggtgcgcg tcgatctcgc
      241 ggccgcgtac cgcctggtgg cggagttcaa gtgggatgac ctcgtcttca cccacatcac
      301 cgcgcgcgtg cccggcaccc acgatcagtt cctcatcaac ccctacggct tcctgttcga
      361 ggagatcacg gcgtccagcc tcgtcaagat cgacctgcag ggcaacaagc tcgacgactc
      421 gccgttcccg gtcaatccgg ccggcttcac gatccacagc gccatccatg gcgccaggca
      481 cgacgcgggc tgcgtgctgc acacgcacac gctcaacggc gtcgcggtct cggcccagaa
      541 gggcggcgtg ctgccgctgt cgcagcagtc gatcttcgtg ctggccagcc tcgcctatca
      601 cgactacgaa ggcgtggccc tgcgcgagga cgagaaggcc cggctgctgc gcgatctcgg
      661 cgacaagcag cacctgatgc tacgcaacca cggcctgctg acgctgggcg cgacggtggc
      721 cgatgccttc ctggcgatgt atctgttcga gaccgtctgc acgatacagg tgagggcgca
      781 gtccggcgga ggcgagctga tcgccatcga tccgcgcatc atcgcgacgg cccgcgagca
      841 agccgcgcag gtcaccaagg gtctgggcag ccagctcacc tggccggggt tgctgcgccg
      901 gctggatcgc aaggacgcga gttatcgatc ctgagccccg ctgcgccggt actgcagcag
      961 cagcttttcc atcgtcgtgt ggcgttcgcg ttcgtgcagc cactgcgtgg gggcgagatt
     1021 gatcgcgcgc acgacggcct cgctgttccc gccatggccg acctccagca cgttgatgca
     1081 ggccggcgcc tgttcgatgc ggccgaagaa ggcacgctgg ccggcgagcg cggccgacag
     1141 cagggcccgg ttgatgccgc cgtgcagcac catcagcagg cagtgccaat ccgggtcggc
     1201 cagccagcgt tcgtaggcgg gcagggcgcg gtccagcatt tcgccgatcg attcgccacc
     1261 caggaagcga tgctcctcga tgtccggccc ggcgcggaag acgccgagaa aggcctgctc
     1321 gatctggtcg cgaggaatgt cggcgagccg gccggagcgg atttcctgca gcgcaggctc
     1381 gatctgcata ctgggtgcgc tggagcccat cgctgcgagc acgcgccgtg ccgtctcgac
     1441 ggtgcgcggc aggccgctcg cgacgacgcg atcgaagcgg atgccgctgg cggcgaacaa
     1501 cgcgccggcc gcatcggcct ggcggcgccc ctcgggattg aggaccacgc cctctggggc
     1561 gaccggcgtg ccatcggcaa ggaaataatc caccgagccg tggcgcatca gatagatgcg
     1621 tctgcggggg gtcatgacga cacccctctc gccggtggct cagcgaaaac gcggcggcct
     1681 tttctcgaag aaggcgcgca ggccttccgc ggcgttgtcg tggaagaggt tgtcgacaaa
     1741 gtgatctctc tcggcatcca gctgggcggc caggtcgcgg ctggggcata ggttcaccag
     1801 ctccttgccg ctggccaggg cattgggcgc aaagccggcg agctgccgcg ccagggccag
     1861 agcttcgttc agggcctggc ccttgtctgt cacgcgattg acaaggccgt gggcctgcag
     1921 ctcgcgtgcc gtcatgggct ccgccagcca gatcagctgc agcgcaaggg cgcgcggcag
     1981 gttctgcacc agatgccagg tcgagccacc gtcgggcgaa aggccgatgc ggttgtaggc
     2041 catcacgaag cgcgcatcct cggccgcgac gagcaggtcg caggccagcg cgatcgagaa
     2101 gcccgcgcca gcggctgccc cttccaccgc agcgatcacc ggcttgggga aggcccgtat
     2161 cgcctcgacg agctggttca gcctggccac gctctcgcgc tgcgtctccc gcccggcctc
     2221 gcggttggcg gccaggcgct gcaggttgcc gccagcgctg aaatggccgc cttcgccggt
     2281 gatgatcacg cagcgcacat cggcgttcgc ctcggccacg cccagggcct ccacgccggc
     2341 gtcgtagacc tggggcgaca gggtattgcg cgtggccgga tcgctgatgg tcaggatcag
     2401 ggcctcttcg cggcgttcgg tgcgcagctc tgcgggcatg gcggcaaggg aagctggggt
     2461 tgagtcagat tgtgtcacca ggcccgccgc atcacaggcg tgccaggcgt tccagcgcgc
     2521 tgcgcagcgt gtcctcgcgc ttggcgaagc
//
LOCUS       DASUCY010000655         2344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_689299, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000655 DASUCY010000000
VERSION     DASUCY010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689299"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..1679
                     /gene="xdhA"
                     /locus_tag="VFV25_12970"
     CDS             90..1679
                     /gene="xdhA"
                     /locus_tag="VFV25_12970"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase small subunit"
                     /protein_id="HEY1394278.1"
                     /translation="MNMQTRPIRFYHHDRGEAVSVDDVAPTRTVLNWLREGAQCTGTK
                     EGCNEGDCGACTVIVAELAEHARSVGTPSAAATIVGGLSLRTVNACIQFLPTLDGKAL
                     LTVEDLQRIAGGTLHPVQQALVECHGSQCGFCTPGFAMSLAASYERHCEAGTRPTRQQ
                     LADDLSGNLCRCTGYRPILDAGQRMFDLPLARLDTRPIVKALRELQQAGGLAYEGPND
                     AVQTEAGPRRDRYWAPRSLDEFAALREQHPQARLLAGCTDIGLWVNKQFRDVGDLLYI
                     GDVDELKRIARRDDGVLRIGAAASLEDAWSALAAELPMLQDMWLRFASLPVRNAGTMG
                     GNIANGSPIGDSAPVLMALGASVALRRGASQRKLALEDFYFDYMKNALQPGEFVEALE
                     VPLPTPATAVRAYKLSKRFDCDISAVAAGLAITLDGDTVKAACFAFGGMAAIVKRAAA
                     AEAAVIGQPWNEATAQAAMAALAQDFKPLADMRASADYRLRSARNLLQRFWLETRRDA
                     PLNAAQLSVWSVMPHAATAAA"
     gene            1697..>2344
                     /locus_tag="VFV25_12975"
     CDS             1697..>2344
                     /locus_tag="VFV25_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdopterin binding
                     subunit"
                     /protein_id="HEY1394279.1"
                     /translation="MNKQVEAFLLEPEQAYADYLRNTRERIDSDGEAQRERQGQRVGI
                     SRPHESAHLHVAGAATYIDDLPELAGTLHAALGLSPVAHGRLKAIDIDKIRALPGVVA
                     VLTAADIPGVNDCGPVIHDDPILAESEVHFLGQPVFAVIATDRELARRAAAQAKQALD
                     IEPLPALLTPQAAHAAQSYVLPPMHMIRGDAKAAIAKAPHRLKGKLSVGGQEQFYL"
BASE COUNT          389 a          851 c          774 g          330 t
ORIGIN      
        1 acccctatac cgactatgtt cgcgagcata tacacctggg cgagcaacgg agtacctttc
       61 gggtatctcc cgacacgttc actctcgcca tgaacatgca aacccggccg atccgcttct
      121 atcaccatga ccgcggagag gcggtcagcg tcgacgacgt ggcgccgacg cgcacggtgc
      181 tgaactggct gcgcgagggc gcgcaatgca cgggcaccaa ggaaggctgc aacgagggcg
      241 actgcggcgc ctgcaccgtg atcgtggccg agctggccga gcatgcacgc agcgtcggca
      301 cgccgtcggc cgcggccacc atcgtcggcg ggctgtcgct gcgcaccgtc aatgcctgca
      361 tccagttcct gccgacgctg gacggcaagg ccttgctcac ggtcgaagac ctgcagcgca
      421 tcgccggcgg cacgctgcat ccggtgcagc aggctttggt ggaatgccac ggctcgcaat
      481 gcggcttctg cacgccgggc ttcgcgatgt cgctggccgc cagctacgag cgccactgcg
      541 aggccggcac gcggcctacg cggcaacagt tggccgacga cctctccggc aacctgtgcc
      601 gctgcaccgg ctaccggccc attctcgacg ccggacagcg catgttcgat ctgccgctgg
      661 cgcggctgga tacccggccc atcgtcaagg cgctgcgcga gctgcagcag gccggcggac
      721 tcgcctacga ggggccaaac gacgcggtgc agacggaagc cggcccgcgc cgcgaccgct
      781 actgggcgcc gcgcagcctg gacgaatttg ccgccctgcg cgagcagcat ccgcaggcgc
      841 gccttctggc cggctgcacc gacatcggcc tgtgggtcaa caagcaattc cgcgacgtgg
      901 gcgacctgct ctacatcggc gacgtcgacg agctcaagcg catagcgcgg cgtgacgatg
      961 gtgtgctgcg catcggcgcc gccgcctcgt tggaggacgc ctggtcggcc ctggccgccg
     1021 agctaccgat gctgcaggac atgtggctgc gcttcgcctc gctgccggtg cgcaatgccg
     1081 gcaccatggg cggcaacatc gccaatggct cgcccatcgg cgacagcgcc ccggtgctga
     1141 tggcgctggg tgcgagcgtg gcgctgcgcc gcggcgccag ccagcgcaag ctggcgctgg
     1201 aggacttcta tttcgactac atgaagaacg cgctgcagcc cggcgagttc gtcgaggcgc
     1261 tggaagtgcc gctgccaacg cccgccaccg cggtgcgggc ctacaagctc tccaagcgct
     1321 tcgattgcga catctccgcg gtcgctgccg gcctggcgat cacgctggac ggcgacaccg
     1381 tgaaggccgc gtgcttcgcc ttcggcggca tggccgccat cgtcaagcgc gccgccgcgg
     1441 ccgaagcagc agtgatcggc cagccctgga acgaagcgac ggcccaggcc gcgatggccg
     1501 cgctggcgca agacttcaag ccgctcgccg acatgcgcgc cagcgccgac taccggctgc
     1561 gcagcgcgcg caacctgctg cagcgcttct ggctggagac gcggcgcgat gcgcctctga
     1621 acgccgccca gctcagcgtg tggagcgtga tgccgcatgc ggccaccgca gccgcctgag
     1681 ccccaaggag cctcggatga acaagcaagt cgaagccttt ctgctcgagc cagagcaggc
     1741 ctatgccgac tacctgcgca acacgcgcga gcgcatcgac agcgatggcg aggcccagcg
     1801 cgagcggcag ggccagcgcg tgggcatcag ccgcccgcat gaatcggccc atctgcacgt
     1861 ggccggcgcg gccacctaca tcgacgattt gccggaactg gccggcacgc tgcacgcggc
     1921 gctcggcctc tcgcccgtgg cgcatggccg gctgaaagcc atcgatatcg acaagatccg
     1981 cgccctgccc ggcgtggtgg ccgtgctgac ggcagccgac atcccaggcg tcaacgactg
     2041 cggacccgtc attcacgacg atcccatcct cgccgaaagc gaggtccatt tcctcggcca
     2101 gcccgtgttc gcagtaatcg ccaccgatcg cgagcttgcg cgccgcgccg ccgcgcaagc
     2161 caaacaggcg ctggacatcg agcccctgcc tgccctgctg acgccccagg cggcccatgc
     2221 cgcgcaaagc tatgtgctgc cgcccatgca catgatccgc ggggatgcga aagccgccat
     2281 cgccaaggca ccgcaccggc tcaagggcaa gctcagcgtc ggcggccagg agcagttcta
     2341 cctg
//
LOCUS       DASUCY010000656         3124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_31228, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000656 DASUCY010000000
VERSION     DASUCY010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31228"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VFV25_12980"
     CDS             complement(<1..554)
                     /locus_tag="VFV25_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEY1394280.1"
                     /translation="MVVLELSASLAQAGPVMDKLRQGGKLVIAHRESSVPFSYVGPDK
                     KPVGYAMDLCLKIAEALRKKLDLKTLPIEYKQVTPSNRIDTIVQHQADMECGSTTNNA
                     ERRQKVAFTIPHYVTGARYMVRADSPIDDLSGFEGKKLVSTKGTTPLTAVDRANRERL
                     LRIQILEAPDHSRAVEMVKNGEAEG"
     gene            complement(636..2051)
                     /locus_tag="VFV25_12985"
     CDS             complement(636..2051)
                     /locus_tag="VFV25_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEY1394281.1"
                     /translation="MSDRSGFRVEHDFLGDKQIPQAAYWGVHSARAVENFQISGQSVG
                     QVSDLVRSLAYVKKAAALTNGELGVLAPELVRAIEQACDDIIRGELHDQFVVDVIQGG
                     AGTSTNMNANEVIANRALEHLGQAKGRYEVLHPNDHVNASQSTNDVYPTALRVAAWFG
                     IEALLEAMAELRGAFEAKAEEFKSILKIGRTQLQDAVPMTLGQEFLAFAIMIGEDEAR
                     LREARALIEEINLGATAIGTGINAPAGYAQRVVSKLAEVSGVPVKPAENLVEATQDTG
                     AFVQLSGVLKRVACKLSKTCNDLRLLSSGPQAGFGDIKLPARQAGSSIMPGKVNPVIP
                     EVMNQVAYEVIGNDVAITMASEAGQLQLNAFEPIMGWSLFKSVRHLTQACKTLRVNCV
                     EGIVANRELLARRVAESVTLVTALNPIIGYEKAALIAKTALAGGGSIAEVAQSLGIMS
                     RAEMEALLVPEKLTQPMRLKA"
     gene            complement(2175..3101)
                     /locus_tag="VFV25_12990"
     CDS             complement(2175..3101)
                     /locus_tag="VFV25_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY1394282.1"
                     /translation="METKWLEDFVSLAETRSFSRSAQLRHVTQPAFSRRIQALEAWAG
                     ADLVDRSSYPTRLTPAGETFLSQALEILGNLQSTRNMMRSHQATGQDMIEFAVPHTLA
                     FAFFPHWLLSLKRDYGPVKSRLIALNVHDAVMRLAEGSCDLLIAYHHPSQPLQLNPER
                     YEMLSLGHETLAPYAKANGDGEPIFRLPGRAGEHIPFLSYASGAYLGRLVDLIIKQAP
                     VAFQLDPIYETDMAEGLKAMALEGHGLAFLPASSVKKELRSRRLVPASPVDTACELTM
                     EVRIYRERPEMARHVKQGSQSLWDYLRGAQEA"
BASE COUNT          490 a         1032 c         1022 g          580 t
ORIGIN      
        1 ccttcggcct cgccgttctt caccatttcg accgcccgcg agtggtcggg cgcttccagg
       61 atctggatgc gcagcaggcg ttcgcggttc gcccggtcga cggccgtcag cggcgtcgtg
      121 cccttggtcg ataccagctt cttgccttcg aagcccgaga ggtcgtcgat ggggctatcg
      181 gcacgcacca tgtagcgcgc cccggtgacg tagtgaggaa tcgtgaaggc caccttctgg
      241 cgccgctcgg cattgttggt ggtcgagccg cattccatgt cggcctggtg ctgcacgatg
      301 gtgtcgatgc gattggacgg cgtcacctgc ttgtactcga tgggcagggt cttgaggtcg
      361 agcttcttgc gcagggcctc ggcgatcttg aggcacaggt ccatcgcata gcccaccggc
      421 ttcttgtcgg gccccacata ggagaagggc accgaggact cgcgatgcgc gatgacgagc
      481 ttgccgcctt gcctgagctt gtccatcacg ggaccggcct gtgccagcga ggccgacagc
      541 tccagcacga ccatccacac gcacggaaat gcgctccaac gacttttggt cttcatttgc
      601 gttcacccat gtttcgggtc gaacatccta agactttaag ctttcagccg catcggctgc
      661 gtcaactttt cgggcaccag cagcgcctcc atctcggcac ggctcatgat ccccaaggat
      721 tgcgccacct cggcgatcga gccgccgccc gccagcgcgg tcttggcgat cagcgcggcc
      781 ttctcatagc cgatgatggg gttcagggcg gtgaccagcg tgaccgactc ggccacccgg
      841 cgcgccagca actcgcggtt ggcgacgatg ccttccacgc agttcaccct cagggtcttg
      901 caggcctggg tcagatggcg cacgctcttg aacagcgacc agcccatgat gggctcgaag
      961 gcgttgagct gcagctggcc ggcctccgag gccatcgtga tggccacgtc gttgccgatc
     1021 acttcgtagg ccacctggtt catcacttcc gggatcacgg gattgacctt gcccggcatg
     1081 atggacgagc cggcctggcg cgcgggcagc ttgatgtcgc cgaagccggc ctgcggcccg
     1141 ctgctgagca gacgcaggtc gttacaggtc ttggacagct tgcaggccac gcgcttgagc
     1201 acacccgaga gctgcacgaa ggcgccggtg tcctgcgtgg cctcgacgag gttctcggcc
     1261 ggcttgaccg gcacgccgct cacctcggcc agcttgctca ccacgcgctg cgcatagccg
     1321 gccggcgcgt tgatgccggt gccgatcgcc gtggcgccca ggttgatctc ctcgatcagc
     1381 gcgcgcgcct cgcgcagcct cgcctcgtcc tcgccgatca tgatcgcgaa ggccaggaac
     1441 tcctggccca gcgtcatcgg cacggcgtcc tgcagctggg tgcggccgat cttgaggatg
     1501 ctcttgaact cctccgcctt ggcctcgaag gcgccgcgca gctcggccat cgcctccagc
     1561 agcgcctcga tgccgaacca ggccgcgacg cgcagcgcgg tgggatagac atcgttggtg
     1621 ctctgcgagg cattgacgtg atcgttcggg tgcagcacct cgtagcggcc cttggcttgg
     1681 cccaggtgct cgagcgcgcg attggcgatc acctcgttgg cgttcatgtt ggtcgaggtg
     1741 cccgcaccac cttggatcac atcgaccacg aattgatcgt gcagctcgcc gcggatgatg
     1801 tcgtcgcagg cctgctcgat ggcgcgaacc agctcgggcg cgagcacgcc gagctcgcca
     1861 ttggtcagcg cggccgcctt cttcacatag gcgagcgagc gcaccagatc ggagacctgc
     1921 cccacgctct ggccggagat ctggaagttc tcgaccgcac gcgcgctgtg cacaccccag
     1981 taggcggcct gcgggatctg cttgtcgcca agaaagtcgt gctcgacacg gaagcctgat
     2041 ctgtcgctca tcgcagtcgc tccttttgct gggcgcgcac tgtaagcccg gccctgcccg
     2101 cacggccaat gctgattgaa gcaagcacca tgccgaatca gcatgatgcc taagtagtaa
     2161 cccgaggccg ggtcttatgc ttcttgcgcg ccccgcagat agtcccacag cgattgggac
     2221 ccttgtttga catgtcgcgc catctcgggg cgctcccggt agatgcgcac ttccatcgtc
     2281 agctcgcagg ccgtgtcgac gggagatgcc ggcaccagcc tgcggctgcg caactccttc
     2341 ttcaccgagc tggcgggcag gaaggcgagg ccatggcctt ccagcgccat cgccttcagg
     2401 ccctccgcca tgtcggtctc gtagatgggg tcgagctgga aggcgaccgg cgcctgcttg
     2461 atgatgaggt cgaccagccg gcccagatag gcaccgctgg catagctcag gaaggggatg
     2521 tgctcgccgg cccgccccgg caggcggaat atcggctcac cgtcgccgtt ggccttggca
     2581 tagggcgcca gcgtctcgtg gcccaggctc agcatctcgt agcgctcggg gttgagctgc
     2641 agcggttgcg aggggtggtg gtaggcgatc agcaggtcgc agctgccttc ggccaggcgc
     2701 atcaccgcat cgtgcacgtt cagcgcgatg agccggctct tgaccggacc gtagtctcgc
     2761 ttcaggctca gcagccaatg cggaaagaag gcgaaggcca gggtatgcgg gacggcgaac
     2821 tcgatcatgt cctgccccgt cgcctgatgg ctgcgcatca tgttgcgggt ggactgcagg
     2881 ttgccgagga tttccagcgc ctgcgagagg aaggtctcgc cggccggcgt cagccgcgtc
     2941 ggataggagg agcgatccac cagatcggcg ccggcccagg cctccagggc ctggatgcgg
     3001 cgcgagaagg ccggctgggt cacatgtctg agctgggccg agcgcgagaa gctgcgcgtc
     3061 tcggccaggc tcacgaagtc ctctagccac ttggtttcca cgcgggccag tgtagcgagg
     3121 cgcc
//
LOCUS       DASUCY010000657         4261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_609094, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000657 DASUCY010000000
VERSION     DASUCY010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609094"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VFV25_12995"
     CDS             complement(<1..180)
                     /locus_tag="VFV25_12995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY1394283.1"
                     /translation="MKNQQPWRPSPLLAASAALHLVLALLIAWQWRWWPGLLALLVLN
                     HLVLMAASLWPRSRLL"
     gene            complement(177..2570)
                     /locus_tag="VFV25_13000"
     CDS             complement(177..2570)
                     /locus_tag="VFV25_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011694403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEY1394284.1"
                     /translation="MMMLQRRAWTRLAAWGLAMLGCVLVILHTRFVSDLSAFMPKMPT
                     ARQQLLVEQLRDGIIARLIMVGIEGADAPQRARLSMELARKLRADESFLGVQNGDAET
                     QDMDKAFFFDNRYLLSPAVQPARFTSEGLHQAIAESLAALSGEGGMLLKHLFTRDPTG
                     ETLALLDQFAGDSQPASREDVWASRDGQRAILLLQTRAAGSDIDAQSQAIQHIRQAFE
                     EIPGRPADAKLVMSGTSVFSVSSRATIEGEVGKLAGAGVVLVLGLLLLVYRSPRLLLL
                     GMLPVASGALVGVAAVSLGFGHVHGLTLGFGTTLIGEAVDYSIYLFIQRAGGHEPSGF
                     WRTIRLGVLTSVAGFAALLFSGFPGLSQLGLYSISGLVTAALVTRHVLPLLMPERLAL
                     RDLSRLGAALEDARVHATRLRWLVYALMVGACAVLAWQHDRIWNRQLSALSPISKAEQ
                     QLDASLRADLGAPDMRYLASFTAPDEQTALQQAERAGAVLRELVQKGQLGGYDSPALV
                     LPSVATQRARQAALPAPEQARAAVDVALAGMPVKPQRLQGFLSDLEAARNRAPLLRAD
                     LKGSSAAMLVDSLLVPRAHDVLVLMPLRSPGSNGEGPHGDLIDVDAVSAAFAAAQLRG
                     ITVIDLLEETTNIFDRYLHEAVLMSGLGALAVVGLLMLGLRSVRRAANVALPLACAVA
                     CVAAGLLLGGHQLTILHLVGLLLVVAVGSNYSLFFDGGMLGQTQDVRRQTLISLVVAN
                     LATVGSFGLLGLSKVPVLSAIGSTVAPGTLLALLFSAMLCRPAEGVARAGATGTMAA"
     gene            complement(2567..3169)
                     /locus_tag="VFV25_13005"
     CDS             complement(2567..3169)
                     /locus_tag="VFV25_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LolA-related protein"
                     /protein_id="HEY1394285.1"
                     /translation="MSKLDPMRRLALAAVLALGFMSCAAAAQDWGLPELMQLLAQHKS
                     AKASFVERKYIAFVDQPIESSGELAFTAPDRLEKRTLAPRPESLVLEGDSLQVEQAGK
                     RRLNISLQSHPEAAAFVESIRGTLAGDLAALQKYYTLDLSGRAERWTLVLVPNQPRMQ
                     KIVSRIRIEGAQSLVKLITFEQADGDRSEMLITAASTSEP"
     gene            complement(3156..4094)
                     /locus_tag="VFV25_13010"
     CDS             complement(3156..4094)
                     /locus_tag="VFV25_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEY1394286.1"
                     /translation="MSVASPTPEWMRRAERGNMFWLRVMSLLSLKLGRRLSRVVLYGI
                     ALYFVLSASAARQASRHYLERCLGRAPSWLDLYRHVLGFASTIHDRVYLLNDRYELFD
                     IQCCGHEAIDSLNREAGQGCFLFGAHLGSFEVLRAAARVARHAAPLKLSIAMYPENAR
                     RINETLAAINPQAQADIIALGRVDSILAVHQRLKEGAMVGILADRAVGPDQCLSLPFL
                     GAPASFPTGPFRLAAMLRQPVYFMTGLYRGGNRYEVHFELLADFSQAQPEGDREEQLR
                     EVLQRYVAMLERHCRSAPYNWFNFYDFWGAPRLEQA"
BASE COUNT          704 a         1470 c         1482 g          605 t
ORIGIN      
        1 gagcagccgg ctgcgcggcc acaggctcgc ggccatgagg accaagtggt tcagcaccag
       61 cagggccagt agcccgggcc accagcgcca ctgccacgcg atcaagagcg cgagcaccag
      121 gtgcagcgct gcgctggcgg caagcaaggg gctgggacgc cagggctgct ggttcttcat
      181 gcggccatcg ttcccgttgc gcccgcgcgc gctacgcctt cggccggccg gcacaacatg
      241 gccgagaaca gcagggccag cagcgtgccc ggcgcgaccg tgctgccgat cgccgacagc
      301 accggcacct tggacaggcc cagcagcccg aagctgccca ccgtcgccag gttggcgacc
      361 accagcgaga tcagcgtctg ccggcgcaca tcctgcgttt ggcccagcat gccgccgtcg
      421 aagaacaggg agtagttcga gcccaccgcc accaccagca gcaggccgac caggtgcagg
      481 atcgtgagtt gatggccgcc gagcagcagg ccggcggcga cacaggccac ggcgcaggcc
      541 agcggcagcg ccacattggc cgcgcgccgc accgagcgca ggccgagcat caagaggccg
      601 accacggcca gcgcccccag gcccgacatc agcaccgctt cgtgcagata gcggtcgaag
      661 atattggttg tttcctcgag caggtcgatg accgtgatgc cccgcagctg cgccgccgca
      721 aatgcggcgg agaccgcatc gacatcgatc agatcgccgt gcgggccctc gccattggag
      781 cccggcgacc gtagaggcat cagcaccagc acgtcgtgcg cgcgaggcac cagcagggaa
      841 tcgaccagca tcgccgccga gctgcccttg aggtccgcgc gcagcagcgg agcgcgattg
      901 cgcgctgcct ccagatcgct caggaagccc tgcagccgct gcggcttgac cggcatgccg
      961 gccagcgcga cgtcgaccgc agcgcgcgcc tgctcgggtg ccggcagggc ggcctggcgg
     1021 gcgcgctggg tcgctacgct gggcagcacc agggccggcg agtcgtagcc gccgagctgg
     1081 cctttttgca ccagctcgcg cagcacggcg ccggcgcgct ccgcctgctg cagggccgtc
     1141 tgctcgtcgg gcgccgtgaa gctggccagg tagcgcatgt cgggcgctcc gaggtcggcg
     1201 cgcaggctgg catcgagctg ctgttcggcc ttggagatgg ggctgagcgc cgacagctgg
     1261 cggttccaga tgcgatcgtg ctgccaggcc agcaccgcgc aggcgcccac catcagcgca
     1321 tagaccagcc agcgcagccg cgtggcatgc acgcgagcgt cttccaatgc ggcaccgagc
     1381 cggctcaggt cgcgcaaagc gagccgctcg ggcatcagca gcggcagcac atggcgcgtg
     1441 accagcgccg ccgtcaccag gccgctgatc gaatacaggc ccagctggga caggcccgga
     1501 aagcccgaga acagcagcgc ggcgaagccc gccaccgaag tcagcacgcc caggcggatg
     1561 gtgcgccaga agccgctcgg ctcgtgcccg ccggcgcgct ggatgaacag gtagatcgaa
     1621 tagtcgaccg cctcgccgat cagcgtcgtg ccgaagccca gggtcagccc gtgcacatgc
     1681 ccgaagccca ggctcaccgc ggccacgccg accagcgcac cgctggccac cggcaacatg
     1741 cccagcagca gcaggcgggg cgaacggtaa acgagcagca gcaggccgag caccagcacc
     1801 acgccggcgc cggcgagctt gccgacttcg ccctctatcg tggcgcggga cgacaccgag
     1861 aacacgctgg tgccgctcat caccagcttc gcatcggcgg gccgccccgg gatctcttcg
     1921 aaggcctggc gtatgtgctg tatcgcctgg ctctgggcgt cgatgtcgga gccggccgcg
     1981 cgcgtttgca gcagcaggat tgcgcgctgg ccgtcgcgcg aggcccagac gtcctccctg
     2041 cttgcgggct ggctgtcgcc ggcaaactgg tcgagcaggg ccagcgtctc gccagtcggg
     2101 tcgcgtgtga acagatgctt cagcagcatg ccgccttcgc ccgacagcgc ggccagcgat
     2161 tctgcaatgg cctggtgcag gccctcgctg gtgaagcgcg ccggctgcac ggccgggctg
     2221 agcaggtagc ggttgtcgaa gaagaaggcc ttgtccatgt cctgcgtctc ggcatcgccg
     2281 ttctgcacgc ccaggaagct ctcgtcggcg cgcagcttgc gcgccagctc catggacagc
     2341 cgcgcgcgct gcggcgcatc ggcgccctcg atgccgacca tgatcagccg cgcgatgatg
     2401 ccgtcgcgca gctgctcgac cagcagttgc tggcgcgcgg tcggcatctt gggcatgaag
     2461 gccgacagat ccgacacgaa gcgcgtgtgc aggatcacca gcacgcagcc cagcatcgcc
     2521 aggccccagg cggcgagccg cgtccacgcg cgtcgctgca gcatcatcat ggctcgcttg
     2581 tgctggcggc ggtgatcagc atctcggagc ggtcgccgtc ggcctgctcg aaggtgatga
     2641 gcttcaccag cgattgcgcg ccctcgatgc ggatacggct cacgatcttc tgcatgcgcg
     2701 gctggttggg caccagcacc agcgtccagc gctcggcgcg accgctcagg tccagcgtgt
     2761 agtacttctg cagcgccgcg agatcgcccg ccagcgtgcc gcgtatgctt tcgacgaagg
     2821 ccgccgcctc gggatgggac tgcaggctga tgttgagccg gcgcttgccg gcctgctcga
     2881 cctgcaggct gtcgccttcc agcaccaggg attcgggcct gggcgccaat gtgcgctttt
     2941 ccagccgatc cggcgcggtg aaggcgagct cgccgctcga ctcgatgggc tggtcgacga
     3001 aggcgatgta tttgcgctcg acgaagctgg ccttggcgga cttgtgctgt gccagcagtt
     3061 gcattagctc gggcagcccc cagtcctgcg cggcggcggc gcaggacatg aagcccaggg
     3121 cgaggacggc tgcaagggcg agcctgcgca tcggatcaag cttgctcaag gcgaggggct
     3181 ccccagaaat cgtagaagtt gaaccagttg tagggcgcgc ttcggcaatg gcgctccagc
     3241 attgcgacat agcgctgcag cacctcgcgc agctgctcct cgcgatcgcc ttccggctgc
     3301 gcctgcgaga aatcggcgag cagttcgaaa tgcacctcgt agcgattgcc gccgcggtag
     3361 agcccggtca tgaaatacac cggctggcgc agcatggccg cgaggcgaaa cggtccggtc
     3421 ggaaagctcg ccggtgcgcc gaggaagggc aggctgaggc attggtcggg gcccacggcg
     3481 cgatcggcca ggatgccgac cattgcgccc tccttgagcc gctgatgcac ggccaggatg
     3541 gagtcgacgc ggcccagcgc aatgatgtcc gcttgcgcct gcgggttgat cgcggccagg
     3601 gtctcgttga tccgcctcgc gttctcggga tacatcgcga tgctgagctt cagcggggcc
     3661 gcgtgcctgg ctactctggc tgctgcgcgc agaacctcga agctgcccag atgggcgccg
     3721 aacaggaagc agccttggcc ggcttcgcgg ttcaggctgt cgatggcctc gtggccgcag
     3781 cattggatgt cgaacagctc gtagcggtcg ttgagcagat agacgcggtc atggatggtg
     3841 cttgcgaagc cgagcacgtg ccgatacaaa tcgagccacg aaggcgcacg ccccaggcag
     3901 cgctcgaggt agtggcgcga ggcttggcgg gcagccgagg cgctcagcac gaaatacagc
     3961 gcgatgccgt acagcacgac gcgcgacagg cggcggccca gtttcagcga gagcaggctc
     4021 atcacgcgca gccagaacat attgccgcgc tccgcgcggc gcatccattc cggtgttggg
     4081 cttgcgacgc tcatgaccac accccgcccg gcgagtacgc cgtgcacccc tccgcgaggg
     4141 ggcgggcctt gcttgggagc ggcccggcgc ggcggcccgt gggatgtctc atgcgccggc
     4201 tcctaggtcg accgcgagaa agcgcccgct ggccacggcg cgctcgccgc agcgcagctc
     4261 g
//
LOCUS       DASUCY010000658         4456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1492338, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000658 DASUCY010000000
VERSION     DASUCY010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492338"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1536
                     /locus_tag="VFV25_13015"
     CDS             <1..1536
                     /locus_tag="VFV25_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEY1394287.1"
                     /translation="QPLQGGQLVMGTLALSLAVFMNVLDTSIANVSLPAIAGDLGVSP
                     NQGTWVITSFAVANAISVPLTGWLTQRFGAVRLFTASVLLFVLSSWLCGFAPSLELLI
                     AFRVLQGLVAGPMIPLSQTLLLSSYPKARAGTALAMWSITTLVAPVVGPLLGGYITDN
                     IAWPWIFYINVPVGLAAAAAAWSIYHKRETPTRKLPIDGVGLGLLVLWVGSMQIMLDK
                     GKELDWFHSGQIVTLALLAVIGFVVFLAWELTEEHPVVDLRLFKKPSFALGSATLAIA
                     YGLFFGNVVLLPLWLQQFMGYTATSAGMALAPVGLFAILLSPVVGKLVGKVDPRRLTT
                     VAFVVFAVVMWMRANFTTNTDMFSILVPTIVQGIAVAFFFIPLMSITLSGLPPERMAA
                     ASGLSNFARITAGAFGTSISTTLWDNRATLHHSHLTDQLGSAASPVFGDALAKLSAGG
                     FSQDQAAAMINRLIDQQAYTRAADDIFLASSVIFVLLIGLVWFAKPSRGGAGGSADAA
                     GAH"
     gene            complement(1615..2601)
                     /locus_tag="VFV25_13020"
     CDS             complement(1615..2601)
                     /locus_tag="VFV25_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY1394288.1"
                     /translation="MSESKPVTMEQIAALAGVSTITVSRALRDSPSVTPATRERIKML
                     AEAAGYRFNLHARNLRLRRSYMIAVMLEMQPDADRPLTDAYPLQLLGGITQELTSRGY
                     SVLLSALQGAGQGPAASADGIVLLGQGANGSAMKTVAAMGLPLVVWGAEHGRGNSVVV
                     GGDNAHGGACAAERFIALGRRELLFLGDVRHAEIRDRQQGYVKTLKKAGLKSHTLTPA
                     AFTFGAGFTAVQAHLARTDTPPDGLFAASDLLAMGAVRAFTDAGLTVPDAVSVIGYDD
                     SPAAQSFAPPLTSVRQDWHHGGVLLARKVLDLIAGQDAASEIMPTELVVRGT"
     gene            2990..>4456
                     /locus_tag="VFV25_13025"
     CDS             2990..>4456
                     /locus_tag="VFV25_13025"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEY1394289.1"
                     /translation="MRPTQVAAPRTGAHLFRPLLRPQLIALAAAALCAAVHAQEPAAP
                     AGKSQEADKSQAQDADKPADKSPEAKEIEAVVVTGTARSKGVRKLEAGYSITTATEEQ
                     IKEATPTSTADILKVVPGVFVETSGGQAGANIRVRGFPTPGDGPYSTFQLNGAPLYHL
                     PTLSFFEHSQLFRLDDTIDRLEVLRGGPSPIFGSGQPGVTVNFIQKDGSNPEGSLRIT
                     TGTGNLMRIDGVYAEPLNDKWSFMVGGFWRQSQGVRDTQFPADDGGQASVVLRRKLDD
                     GSLQLYARALHDRNAFYTAIPVVGGADGKSVSSYPGFNALYDYFYGNELRNIVLETGR
                     ATGRTLPNGKPEILRSTVTKDLADGRGADVQTYGANLDTRLGDWTLANKFGHTSGEVP
                     TYALFSGANPQTLGSYIASQVTAANGDPNVVAAAGRPATGGTATFTNGGGAITNMAMP
                     VIVNGMWAVDKTLRSTSNETRLSKEVAPGNNLTLGLYLA"
BASE COUNT          695 a         1569 c         1466 g          726 t
ORIGIN      
        1 caaccgctcc aaggcggcca actcgtcatg ggcaccctcg ccttgtcgct ggccgtgttc
       61 atgaacgtgc tcgacacgtc gatcgcgaac gtgtcgctgc cggccatcgc cggcgacctg
      121 ggcgtcagcc ccaaccaggg cacctgggtc atcaccagct tcgcggtcgc caacgcgatc
      181 tcggtgccgc tcaccggctg gctcacgcag cgcttcggcg ccgtgcgcct cttcaccgcg
      241 agcgtgctgc tcttcgtgct cagctcctgg ctgtgcggct tcgcgccctc gctcgaactg
      301 ctgatcgcct tccgcgtgct gcaaggcttg gtggcggggc cgatgatccc gctgtcgcag
      361 accctgctgc tgtccagcta ccccaaggcc agggccggca cggcgctggc gatgtggtca
      421 atcacgacac tggtggcgcc ggtggtcggg ccgctgctgg gcggctacat caccgacaac
      481 atcgcctggc cctggatctt ctacatcaac gtgccggtgg gcctggccgc cgccgcggcg
      541 gcctggagca tctatcacaa gcgcgagacg cccacgcgca agctgcccat cgacggcgtg
      601 ggcctggggc tgctggtgct gtgggtcgga tccatgcaga tcatgctcga caagggcaag
      661 gagctcgact ggttccactc cggccagatc gtcacgctgg ccctgctggc ggtgatcggt
      721 ttcgtggtct tcctcgcctg ggagctgacc gaggagcacc cggtggtgga cctgcgcctc
      781 ttcaagaagc ccagcttcgc tttgggctcg gccacgctgg cgatcgccta cgggctcttc
      841 ttcggcaatg tggtgttgct gccgctgtgg ctgcagcagt tcatgggcta caccgccacc
      901 tccgccggca tggcgctggc gccggtcggg ctgttcgcga tcctgctctc gccggtggtc
      961 ggcaagctgg tcggcaaggt cgacccgcgg cgcctgacca cggtggcctt cgtcgtgttc
     1021 gcggtcgtga tgtggatgcg cgccaacttc acgaccaaca ccgacatgtt cagcatcctc
     1081 gtgcccacca tcgtgcaggg catcgcggtg gccttcttct tcatcccgct gatgagcatc
     1141 acgctctccg ggctgccgcc ggagcgcatg gccgctgcct cgggcctgag caacttcgcg
     1201 cgcatcacgg ccggcgcctt cggcacctcg atctccacca cgctgtggga caaccgcgcc
     1261 acgctgcacc acagccatct gactgatcag ctgggcagtg cggccagccc ggtgttcggc
     1321 gatgcgctgg ccaagctctc ggccggcggc ttcagccagg accaggcggc ggccatgatc
     1381 aaccgcttga tcgaccagca ggcctatacc cgcgccgcgg acgacatctt cctcgcctcg
     1441 tccgtcatct ttgtgctgct gatcggcctg gtgtggttcg ccaagccctc gcgaggcggt
     1501 gccggcggtt cagcggatgc ggccggggcg cactgaaagc gcgcccgctg tcgcggggcg
     1561 tcgcacgaag tgttgaacag gggctccgtc gcggagcccc tgttcgttgc gcactcaggt
     1621 gccgcgcacg acgagctcgg tcggcatgat ctcgctggcc gcgtcctggc cggcgatcag
     1681 gtccagcacc ttgcgtgcga gcagcacgcc gccgtggtgc cagtcttggc gcacgctggt
     1741 cagcggcggc gcgaagctct gggcggcggg cgagtcgtcg tagccgatca cggagaccgc
     1801 atcgggcacc gtcaggccgg catcggtgaa agcgcgcacg gcgcccatcg cgagcaggtc
     1861 gctggcagcg aagaggccgt cgggcggcgt gtcggtccgg gccagatgag cctgcacggc
     1921 cgtgaagccg gcaccgaaag tgaaggcagc cggggtcagc gtgtgcgact tgagaccggc
     1981 tttcttcagc gtcttcacgt agccctgctg ccgatcgcgg atctcggcgt gacgcacgtc
     2041 gccgaggaag agcagctcgc gccggcccag ggcgatgaag cgctcggccg cgcaggcgcc
     2101 gccgtgggcg ttgtcgccgc cgacgacgac cgagttgccg cgcccatgtt cggctcccca
     2161 gacgacaagg ggcaggccca ttgcggcgac ggtcttcatg gctgagccgt tggcgccctg
     2221 gccgagcagg acgatgccgt cggccgacgc agccgggccc tggccggcgc cttgcagggc
     2281 agacagcagt acgctgtagc cgcgcgaggt cagctcctgc gtgatgccgc cgagcagctg
     2341 cagcgggtag gcatcggtca gcggccggtc ggcatcgggc tgcatctcca gcatgactgc
     2401 gatcatgtag ctgcgccgca gccgcagatt gcgggcatgg aggttgaagc ggtagccggc
     2461 ggcctcggcc agcatcttga tgcgctcgcg cgtggccggc gtcaccgagg ggctgtcgcg
     2521 cagcgcacgc gacacggtga tggtgctgac gccggcgagc gcagcgatct gctccatcgt
     2581 gaccggcttc gattcgctca tgcccgctct cggtttcagt cgtttgcgct gtcgtgctgg
     2641 cccaggcgct cgacgagcag gggcaagcat acggtccgct cgtcgtgcag cttgtatggc
     2701 gggttcatga cgagcatagc cttgcccatc atatcgaagc ccctggcatc ggcctgggcc
     2761 gcggcaagcg cgtgcagcca gcccgtcttc gcggtcgcgc cggctcaggc catgaggcgc
     2821 tgcagtgcct cgcagtggaa acggtgatga tatcgatgtc atacgattgc aacactggtg
     2881 tttacccaaa tgacatcgat aacattttgg atgcacagtc tagcccatcg aaagtgggcg
     2941 acggccgcgc cggctttcgg gaatcaccac aatcaacgga gacaacccga tgcgacccac
     3001 tcaagtggcc gcgccgcgca ccggcgctca tctgttccga cctctcttgc gcccccagct
     3061 gatcgcgctg gctgccgccg cgctctgtgc cgccgtccac gcccaggagc ccgccgcgcc
     3121 ggccggcaaa tcccaggagg ccgacaaatc ccaggcccag gacgccgaca agccggccga
     3181 caagtccccc gaggccaagg agatcgaagc cgtcgtcgtc accggcacgg cgcgcagcaa
     3241 gggcgtgcgc aagctggagg ccggctactc gatcacgacc gcgaccgagg agcagatcaa
     3301 ggaggccacg ccgacgagca cggccgacat cctgaaggtc gtgcccggcg tcttcgtcga
     3361 gacctcgggc gggcaggccg gcgccaacat ccgcgtgcgc ggcttcccga cgccgggcga
     3421 tggcccctat tcgaccttcc agctcaacgg cgcgccgctc taccatctgc caacgctgag
     3481 cttcttcgag cactcccagc tctttcggct cgacgacacg atagaccgcc tggaggtgct
     3541 gcgcggcggc cccagcccca tcttcggcag cggccagcct ggcgtgacgg tcaacttcat
     3601 ccagaaggat ggcagcaatc ccgagggcag cctgcgcatc accaccggca cgggcaacct
     3661 gatgcgcatc gacggcgtct atgccgagcc acttaacgac aagtggtcct tcatggtcgg
     3721 cggcttctgg cgccagagcc agggcgtgcg cgacacgcag ttcccggccg acgacggcgg
     3781 ccaggccagc gtcgtgctga gacgcaagct cgatgacggc tcgctccagc tttatgcccg
     3841 cgccctgcac gaccgcaatg ccttctacac ggccatcccg gtcgtcggcg gcgcggatgg
     3901 caagagcgtg tccagctacc ccggcttcaa cgctctgtac gactacttct acggcaacga
     3961 gctgcgcaac atcgtgctcg agaccggccg cgcgaccggc cgcacgctgc cgaacggcaa
     4021 gcccgagatc ctgcgcagca cggtcaccaa ggacctcgcc gacggccgtg gcgccgatgt
     4081 gcagacctat ggcgccaacc tcgataccag gctcggcgac tggaccttgg ccaacaagtt
     4141 cggtcacacg agcggcgagg tgccgaccta tgccctgttc tccggcgcca acccgcagac
     4201 cctgggcagc tatatcgctt cgcaagtcac tgccgcgaac ggcgatccga atgtcgtcgc
     4261 cgcggccggc aggcccgcga ccggcggcac ggcgacattc acgaacggcg gcggcgccat
     4321 caccaacatg gccatgcccg tcatcgtcaa cggcatgtgg gcggtcgaca agacgctgcg
     4381 ctcgacgagc aacgagacgc gcctgtcgaa ggaggtcgcc cccggcaaca acctgaccct
     4441 cggcctctac ctggcc
//
LOCUS       DASUCY010000659         2470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1572900, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000659 DASUCY010000000
VERSION     DASUCY010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1572900"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            447..1001
                     /locus_tag="VFV25_13030"
     CDS             447..1001
                     /locus_tag="VFV25_13030"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEY1394290.1"
                     /translation="MTTPATTAVPAPRRGLRLLIVGLLVGVLGSIGLTAWAQGEHGMG
                     APHGGGMMFMGSPEHVDHMVDHMLKGLNATDAQRAQIKKIAEAAATDLKAQHEAAKGL
                     HEQGMAIFTAPTVDANAAEALRQKMLAQHDQASKRVLQAMLDIANVLTPEQRAKFGER
                     MKQRHEMMMRHMRERQQLEKSQGN"
     gene            1134..1844
                     /locus_tag="VFV25_13035"
     CDS             1134..1844
                     /locus_tag="VFV25_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY1394291.1"
                     /translation="MTTARLLLIDDDLRLTGMVGDYLRSSGFEVDVAPTLGAGRQALA
                     RETYDALVLDLMLPDGDGLDLCRELRANSRTRALPLLMLTARGEPMDRILGLELGADD
                     YLPKPFEARELLARLKALLRRASPDLAADDVLRFGRLEIDLGARQARLGGVPCELTSY
                     QFDLLLVLAQSPGRVLSRDQIMDSLKGHPLEAFDRSIDVHVSRIRAVIEDDPKNPRRI
                     LTVRGVGYVFARKQDAES"
     gene            1885..>2470
                     /locus_tag="VFV25_13040"
     CDS             1885..>2470
                     /locus_tag="VFV25_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEY1394292.1"
                     /translation="MRSLYLRIYLTVVLVLLAFALVAGWMFHRHIEQERAHFELTIAE
                     RMAAWATLIENSLPAATAGQAEQRDALIEWGERLHIPLALDDAKGERIATSAAFAARE
                     AELGGPPKRAQRVVLSDGRALWIWRSSAMLRGGGPPPQPGDERRPPPGGDDPPPPPRE
                     VGMMALPAFPSFLLIGGGAGLVLTLALLFVAVAAG"
BASE COUNT          385 a          882 c          832 g          371 t
ORIGIN      
        1 ttgaaggtcg tgccggccgt gaagcgcgcc gggtcgaagc cctcggggct gccttcggag
       61 caatagatca gggtgctggc ggcctgggcg ccgaaagccg tgaaagcggc ggcgcaggcg
      121 gtgagggtag ccgccagggt agggcggacg agcgacatca tgtgcttcat gaaaaaagcc
      181 accttgtggg tgagaaagtc gcgcgagtct agggcaagcg cgagggcgcg ctattgtggc
      241 aagcgcgggc ttgcattctg gcccaggcgg cgtgcccctt ggccgtctgc gccaagtcct
      301 acctgctgtg gcgcgctcgc gacgcaaaca ggttctgagc gcgccgattc acagatcttt
      361 acccctgccg cacaaggtcg acaccgctgc acccgacatt gctccccagg gcatcacaga
      421 cctgcccacc aatcaaggag caaagaatga ccacccccgc taccaccgca gttcctgccc
      481 ctcggcgcgg cctgcgcctg ctcatcgtcg gcctgctggt cggcgtgctc ggcagcatcg
      541 ggcttactgc ctgggcacag ggcgagcatg gcatgggtgc cccgcatggc ggcggcatga
      601 tgttcatggg ctcgcccgag catgtcgacc acatggtcga ccacatgctg aagggcctga
      661 atgccaccga cgctcagcgc gcccagatca agaagattgc cgaggctgcg gccaccgacc
      721 tcaaggccca gcacgaggcc gccaagggcc tgcacgagca gggcatggcg atcttcacgg
      781 cgcccacggt cgacgccaac gccgcggagg cgctgcgcca gaagatgctg gcccagcacg
      841 accaggcttc caagcgcgtg ctgcaggcga tgctggacat cgccaatgtg ctcacgcctg
      901 agcagcgcgc caagttcggc gaacgcatga agcagcgcca cgaaatgatg atgcgccata
      961 tgcgggagcg ccagcagctc gaaaagtccc agggcaactg aacacaggag ctgaggcggc
     1021 cgccgcgcga gcggccggcc gccttgtggc ttatgcttga caccaaggtg attcccccag
     1081 gcgccccgca agcggggcgt ttgtcatctt ccttccattc cgatgtggcc cccatgacca
     1141 cggcacgctt gctcctcatc gacgatgacc tgcgcctcac cggcatggtg ggcgactatc
     1201 tgcgcagctc cggcttcgag gtcgatgtcg cgcccacgct gggcgccggg cgccaggccc
     1261 tggcgcgcga gacctatgac gcgctcgtgc tcgacctgat gctgcccgat ggcgatggcc
     1321 tggacctgtg ccgcgagctg cgcgccaact cgcgcacgcg ggccctgccg ctgctcatgc
     1381 tcaccgcccg cggcgagccc atggaccgca tcctcggcct ggagctgggc gcagacgact
     1441 acctgcccaa gcccttcgag gcgcgcgagc tgctggcacg cttgaaggcc ttgctgcgcc
     1501 gcgcctcgcc ggacctcgcg gccgacgacg tgttgcgctt cggccgcctg gagatcgacc
     1561 tcggcgcacg ccaggcccgc ctgggcggcg tgccctgcga gctgaccagc tatcagttcg
     1621 acttgctgct ggtgctggcg caaagcccgg gccgcgtgct cagccgcgac cagatcatgg
     1681 actccctcaa gggccatccg ctggaggcct tcgaccgctc catcgacgtg cacgtctcgc
     1741 gcatccgcgc cgtgatcgag gacgacccca agaacccgcg gcgcatcctc acggtgcgcg
     1801 gcgtgggcta cgtcttcgcc aggaagcagg acgcggaatc gtgacggcgc cggccaggcg
     1861 ccgggtcgca tcatcggcat cggcatgaga tcgctctacc tgcgcatcta cctcacggtg
     1921 gtgctggtgc tgctggcctt cgcgctggtc gcgggctgga tgttccaccg ccacatcgag
     1981 caggagcgcg cgcacttcga gctcaccatc gccgagcgca tggcggcctg ggccacgctg
     2041 atcgagaaca gcctgcccgc ggccaccgcg ggccaggccg agcagcgcga tgcgctgatc
     2101 gaatggggtg aacgcctgca catcccgctc gcgctggacg atgccaaggg cgagcgcatc
     2161 gccacctcgg cggccttcgc cgcccgcgag gccgagctcg gcggcccgcc caagcgagcg
     2221 cagcgcgtgg tgctgtccga cggccgcgct ttgtggatct ggcgctcgag cgccatgctg
     2281 cgcggcggcg gaccacctcc gcagcccggc gacgagcgcc gaccacctcc cggcggcgac
     2341 gacccgcccc cgcccccgcg cgaggtcggc atgatggcct tgccggcctt tccgtccttc
     2401 ctgctgatcg gcggcggggc ggggctggtg ctcacgctcg cgctgctgtt cgtcgccgtc
     2461 gcggccgggg
//
LOCUS       DASUCY010000660         5440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_304153, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000660 DASUCY010000000
VERSION     DASUCY010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 5440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5440
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304153"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..737
                     /locus_tag="VFV25_13045"
     CDS             156..737
                     /locus_tag="VFV25_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEY1394293.1"
                     /translation="MNSSSGTAPQRASARFMLAHPAHLLALGFGSGLSPVAPGTVGTL
                     WAWASYLVLDLFLGPMQWAGLLAAGLAVGWWACTRTAQHLGVADPSPVVWDEILAFWA
                     MLWLVTPTGVHVDAGWQVVAGSMLAPQIVAFVLFRFFDALKPGPVAWADALFKHVRRP
                     DGGIGWAQGFGIIFDDIVAALCALLVMALWKAL"
     gene            746..1249
                     /locus_tag="VFV25_13050"
     CDS             746..1249
                     /locus_tag="VFV25_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003068320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEY1394294.1"
                     /translation="MQSQEDLIVQLAESLRARGWRMAAAESCTGGLIAATCTSIAGSS
                     DWFERGFVTYSNEAKTEMLGVPAELIARCGAVSREVALAMADGALRHARAELVVAVTG
                     IAGPSGGSPEKPVGTVWLAWATRDGAVEAELLRLDGDRGEIRAQTVLEALQRLLRAAR
                     ASGRAFP"
     gene            1246..2184
                     /locus_tag="VFV25_13055"
     CDS             1246..2184
                     /locus_tag="VFV25_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase A"
                     /protein_id="HEY1394295.1"
                     /translation="MSILLAARFADADERAAWQAALSAALPGEHFADAVGGAEAAAID
                     IAIVANPPPGALQGLPGLKLIQSLWAGVDKLLADPSLPAGVPIARMVDPAMNAAMAET
                     ALWAVLGLHRGYFRYAQQQRRARWAQLPQRRADEITVAVLGLGQMGRCTAQRLVVQGY
                     RVRGWSTRTAALEGVETHTGLAALPALLARADVVVNLLPLTPETRGLFAAERFAQMRA
                     GASLVNLARGAHVVDADLLAALDSGRLAHAVLDVFNAEPLPADHPYWRHPGVTVLPHA
                     AALTDPRSAAQIAAANVRALREGRPLGHLVDRARGY"
     gene            complement(2191..3273)
                     /gene="holA"
                     /locus_tag="VFV25_13060"
     CDS             complement(2191..3273)
                     /gene="holA"
                     /locus_tag="VFV25_13060"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEY1394296.1"
                     /translation="MQVRADQLASHLKKGLRPIYTLHGDEPLLAQEAADAIRAAARAA
                     GHTERQVHTVAGAHFDWSSLLGAASAMSLFAERQLIEIRIPSGKPGKEGSEALQRYCE
                     HLSEDVITIVALPRLDGTQLKSAWFGALDAAGVTLRLDAIERQALPQWIAQRLSAQGQ
                     RVQDGEPGQRTLAFFADRVEGNLLAAHQEIAKLGLLYPQGELSFEDVEAAVLNVARYD
                     VFKLGEAVLSGQVARVLRMLDGLQSEGEAAVLVHWTLAEDIRALKRVKDAMAAGKPMP
                     LALRENRVWGVKERLFERALPLLGDAALAHLVEAAQICDGLVKGLKHPAWPTDPWQGL
                     QRLALLLCQQLAGPSRDRPLKLALEV"
     gene            complement(3290..3778)
                     /gene="lptE"
                     /locus_tag="VFV25_13065"
     CDS             complement(3290..3778)
                     /gene="lptE"
                     /locus_tag="VFV25_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEY1394297.1"
                     /translation="MQRRTWLGIASAAMLTACGFELRRPPELQLHSVQLKGFAPRSPL
                     AEELKQQLQASGSTRVVESAAQAEAILESLIEAREQVVVASTAAGQVRELTLRLRFKF
                     RLATPGGNELIAPTELLLSNDLSYIETSALAKEQEAEMLYRAMQSDIVSQVMRRLAAV
                     RI"
     gene            complement(3778..>5440)
                     /gene="leuS"
                     /locus_tag="VFV25_13070"
     CDS             complement(3778..>5440)
                     /gene="leuS"
                     /locus_tag="VFV25_13070"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEY1394298.1"
                     /translation="TEEKVEVWVGNYVLMSYGDGAVMGVPAHDERDFAFAKKYGIDIR
                     HVIHVDGEHFSYDHWQDWYADKQRGVTINSENFSGLGYQAAVDAIAAALEHKGLGEKK
                     TTWRLRDWGISRQRYWGTPIPIIHCETCGSVPVPEKDLPVILPEDLIPDGSGNPLNKC
                     ESFLKVACPKCGKPARRETDTMDTFVDSSWYFMRYCDARNDRAMVGAGTQYWMPMDQY
                     IGGIEHAILHLLYARFWTKVMRDLGFVKADEPFTKLLTQGMVLKGAFFHKPEGGGKHY
                     YWEHEVDVLTNEHDQVVGGKLKADGTPLEFEMTTMSKSKNNGVDPQDLIDRYGADTAR
                     LFVMFASPPEQTLEWSDSGVEGAHRFLKRVHTFGQKHAAAITGAHDIGGALSAEAKAL
                     RFEVHSVLKQVSYDYERMQYNTVVSGAMKLLNALEAYKGDASAASHAVLREGYSVLLR
                     ALYPACPHLTHALWKQLSYAARLGDLLDAPWPQVDEAALVQDEIELMLQVNGKLRGSI
                     KVAAAADKAAIEAAALAAPEFAKFSEGRPAKKVIVVPGRLVNVVI"
BASE COUNT          797 a         1866 c         1850 g          927 t
ORIGIN      
        1 gaggccgcgc ggaaacgcgt cgtcgaagcc gcgcgatcgg ccagcgtcgg cgtcacgcgc
       61 atcggccgga ccgaggccaa gcccgggctc aggctggtgg atcgcgaagg gcatgccctc
      121 gaagcggcct ggacctcctt cgaccacttc gcggcatgaa cagtagcagc ggcaccgcgc
      181 cacaacgcgc cagcgcgcgt ttcatgcttg cacacccggc gcatttgctc gcgctgggct
      241 ttggcagcgg actcagtccg gtcgcgccgg gcacggtcgg cacgctgtgg gcctgggcca
      301 gctacctcgt actcgatctc tttctcggcc cgatgcaatg ggccgggctg ctggcggcag
      361 ggctggccgt tggctggtgg gcctgcacac gcaccgccca gcatctgggc gtggccgacc
      421 ccagccccgt ggtatgggac gagatcctcg ccttctgggc catgttgtgg ctggtcacgc
      481 ccacgggggt gcatgtcgac gcgggctggc aagtcgtggc aggcagcatg ctcgcgccgc
      541 agatcgtcgc cttcgtgctg ttccgcttct tcgacgcgct caagcccggg ccggtggcct
      601 gggccgatgc cttgtttaag catgtacgcc gccccgatgg cggcatcggc tgggcccagg
      661 gtttcggcat catcttcgac gacatagtcg ccgcgctgtg cgccctgcta gtcatggctc
      721 tctggaaggc cctctgagca aggctatgca atcgcaagaa gatctcatcg tccagctcgc
      781 cgagtcgctg cgcgcgcgcg gctggcgcat ggccgcggcc gagagctgca ccggcggcct
      841 gatcgccgct acgtgcacga gcatcgccgg ctcgagtgac tggttcgagc gtggcttcgt
      901 cacctacagc aacgaggcca agaccgagat gttgggcgta ccagccgagc tgatcgcccg
      961 ctgcggtgcg gtgagccgcg aggtggcgct ggcgatggcg gacggggcgc tgcgccatgc
     1021 ccgcgcggaa ctggtcgtgg ccgtgaccgg catcgccggc ccgagcgggg gctcgccgga
     1081 aaagccggtg ggcacggtct ggctggcctg ggcgacgcgc gacggcgcgg tcgaggcgga
     1141 actgctgcga ttggacggcg atcgcggcga gatacgcgcg cagaccgtgc tggaagcgct
     1201 gcagcggctg ctgcgggcag cgcgtgcttc cggtcgagca ttcccatgag catcttgctc
     1261 gccgcacgct tcgcagacgc cgacgagagg gccgcctggc aggccgcgct cagcgctgca
     1321 ctgcccgggg agcatttcgc ggatgcggtg ggaggcgctg aggccgccgc catcgacatc
     1381 gccatcgtcg ccaatccccc tcccggcgcc ttgcagggcc tgcccggctt gaagctgatc
     1441 cagtcgctat gggcaggcgt ggacaagctg cttgccgacc ccagcctacc ggccggcgtg
     1501 ccgatcgctc gcatggtcga tccggccatg aatgccgcga tggccgagac ggcgctgtgg
     1561 gcggtgctcg gcttgcaccg cggttatttc cgttacgcgc agcaacagcg cagggctcgc
     1621 tgggctcagc tgccgcagcg gcgcgccgat gagatcacag tcgccgtgct ggggctgggg
     1681 cagatgggcc gctgcacggc gcagcgcttg gtggttcagg gctaccgcgt gcggggctgg
     1741 agcacgcgca ctgccgcgct ggagggcgtg gagacccata ccggcctcgc ggccctgccc
     1801 gcgctgctgg cgcgcgccga cgtcgtcgtc aacctgctgc ccttgacgcc cgagacgcgc
     1861 ggcctgttcg cggccgaacg cttcgcccag atgcgcgcgg gcgccagcct ggtcaacctg
     1921 gcgcgcggcg cccatgtggt cgatgccgat ctgctggccg cgcttgacag cggccggctc
     1981 gcgcatgccg tgctcgatgt cttcaacgcc gagccgctgc ccgcggatca tccgtattgg
     2041 cgccatcccg gtgtcacggt gctgccgcat gccgcagcac tcactgaccc gcgcagcgcg
     2101 gcccagatcg cggcggccaa tgtgcgggca ctgcgcgagg gccgaccgct gggccatctg
     2161 gtcgaccggg cgcgcggtta ctgaaccgat tcagacctcc agcgccagct tgagcggtct
     2221 gtcgcgactg ggtccggcca gttgctggca cagcagcagc gccaggcgct gcaggccctg
     2281 ccaggggtcg gtcggccagg cgggatgctt gagccccttg acgagcccgt cgcagatctg
     2341 cgccgcctcc acgagatgcg cgagggcggc atcgccgagc agcggcagcg cacgctcgaa
     2401 gaggcgctcc ttgacacccc agacgcggtt ctcgcgcagc gccagcggca tgggcttgcc
     2461 cgcggccatg gcgtccttga cccgcttcag cgcgcggatg tcctcggcca gggtccagtg
     2521 caccagcacc gcggcctcgc cttcggactg cagaccgtcc agcatgcgca gcacgcgtgc
     2581 cacctggccc gagagcaccg cctcgccgag cttgaagacg tcgtagcgcg cgacgttgag
     2641 caccgccgcc tcgacgtcct cgaagctcag ctcgccctgc ggatagagca gcccgagctt
     2701 ggcgatctcc tgatgcgccg ccaacagatt gccctcgaca cgatcggcaa agaaggcaag
     2761 ggtgcgctgg ccgggctcgc cgtcctgcac gcgctggcct tgcgccgaca ggcgctgcgc
     2821 gatccattga ggcagcgcct ggcgctctat ggcgtccaac ctgagcgtga cgccggcagc
     2881 gtcgagagcg ccaaaccacg cgctcttgag ctgcgtgccg tccaggcgcg gcagcgcgac
     2941 gatagtgatc acgtcctcgc tcagatgctc gcaatagcgc tgcagggcct ccgagccctc
     3001 cttgcccggc ttgccggagg gaatgcgtat ctcgatgagc tggcgctcgg cgaacaggct
     3061 catcgcgctg gctgcgccga gcagcgaact ccagtcgaaa tgggcgccgg ccacagtgtg
     3121 cacctggcgc tcggtgtggc ccgccgcgcg agccgcggcg cgtatcgcat cggccgcctc
     3181 ctgcgccagc agcggctcgt cgccgtgcag cgtgtaaatg ggacgcaggc ccttcttcag
     3241 gtgggatgcg agctggtcgg cgcgaacttg catggctgca ggcgccgctt cagatcctga
     3301 cggccgccag gcggcgcatc acctgggaga cgatgtcgct ctgcatcgca cgatagagca
     3361 tctcggcctc ctgttccttg gcgagtgcac tcgtttcgat gtagctcagg tcgttgctga
     3421 gcagcagctc ggtgggcgcg atcagttcgt tgccgccggg cgtcgcgagt cggaacttga
     3481 agcgcaaccg cagcgtcagc tcgcgcacct ggcccgccgc ggtcgaggcc accacgacct
     3541 gctcgcgcgc ctcgatgagg gattcgagga tggcctcggc ctgggccgca ctctccacca
     3601 cgcgcgtgga gccgctggcc tgcaactgct gcttcagctc ctccgccagc ggcgagcgcg
     3661 gcgcgaagcc cttgagctgt acgctgtgca gctgcagctc gggcggccgc cgcagctcga
     3721 agccgcaagc ggtgagcatg gccgccgatg cgatgccgag ccaggtgcgg cgctgcatca
     3781 gatgacgacg ttgaccagcc gcccgggcac cacgatcacc ttcttggccg gcctgccttc
     3841 gctgaacttg gcgaactcgg gcgcagcaag ggcggcagcc tcgatggccg ccttgtcggc
     3901 cgcggccgca accttgatcg agccgcgcag cttgccgttg acctgcagca tcagctcgat
     3961 ctcgtcctgc accagcgccg cttcgtcgac ctgcggccag ggcgcgtcga gcagatcgcc
     4021 cagcctggcg gcatagctca actgtttcca cagcgcatgc gtgagatgcg ggcaggccgg
     4081 atagagcgcg cgcagcaaca ccgaatagcc ttcacgcagc actgcatgcg aggccgcgct
     4141 ggcgtcaccc ttgtaggcct ccagcgcatt gagcagcttc atcgcgccgg acaccacggt
     4201 gttgtactgc atgcgctcgt agtcgtagct cacctgcttg agcacgctat gcacctcgaa
     4261 gcgcagtgcc ttggcctcgg cgctcagcgc accgccgatg tcgtgggctc ccgtgatggc
     4321 ggccgcatgc ttctggccaa aggtgtggac gcgcttcagg aagcggtgcg cgccttcgac
     4381 gccggagtcg ctccattcca gcgtctgctc gggcggcgac gcgaacatca cgaacagccg
     4441 cgccgtatcg gcgccgtagc gatcgatgag gtcctgggga tcgacgccgt tgttcttgga
     4501 cttcgacatc gtcgtcatct cgaactcgag cggcgtgccg tcggccttga gcttgccacc
     4561 gaccacctgg tcgtgttcat tggtgagcac gtcgacctcg tgctcccagt agtagtgctt
     4621 gccgccgccc tcgggcttgt ggaagaaggc acccttgagc accatgccct gcgtcagcag
     4681 cttcgtgaag ggctcatccg ccttcacgaa accgaggtcg cgcatcacct tggtccagaa
     4741 gcgcgcatac agcaggtgca ggatggcgtg ctcgatgccg ccaatgtatt ggtccatcgg
     4801 catccagtac tgcgtgccgg cgccgaccat cgcgcggtcg ttgcgcgcgt cgcagtagcg
     4861 catgaagtac cacgacgagt cgacgaaggt gtccatcgtg tcggtctcgc gccgtgccgg
     4921 cttgccgcac ttcgggcaag cgaccttcag gaaggattcg catttattga gcgggttacc
     4981 gctgccgtcg ggaatcaagt cttcaggcag tatgaccggc aggtctttct cgggtaccgg
     5041 taccgagccg caagtttcgc aatggatgat ggggatcggc gtgccccagt agcgctggcg
     5101 gctgatgccc cagtcgcgaa ggcgccaggt ggtcttcttc tcgcccaggc ccttgtgctc
     5161 gagcgcggcc gctattgcgt ccaccgccgc ctggtagccg aggccgctga agttttcgga
     5221 attgatcgtg acgccgcgct gtttgtcggc gtaccagtcc tgccaatggt cgtagctgaa
     5281 atgctcgccg tcgacgtgga tcacgtgccg gatatcgatg ccgtacttct tcgcgaaggc
     5341 gaagtcgcgc tcgtcgtgcg ctggcacgcc catcacggcg ccgtcgccat agctcatcag
     5401 cacatagttg ccgacccaga cctcgacttt ctcctctgtc
//
LOCUS       DASUCY010000661         2092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_850653, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000661 DASUCY010000000
VERSION     DASUCY010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850653"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1271)
                     /locus_tag="VFV25_13075"
     CDS             complement(<1..1271)
                     /locus_tag="VFV25_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEY1394299.1"
                     /translation="MQRLHWRRALGLALGLCYAAAAGAGGPGYRQVQLDLARAAGPVD
                     RFFDLSVGSDFPGTLIRDDSQAQLKTAVDELGFRYVRFHAIFHDVLHTVREAEGRLVF
                     DFSGIDKLYDALLAKGIRPFVELGFTPEAMASSKQTIFYWKGNTSHPKPEAWQALVEA
                     FVRHLRERYGVEEVRRWPFEVWNEPNLDGFWERADQQAYFELYARTAKAIKAIDPQLR
                     VGGPSTAGAAWVPEFLAYAKAHDAPVDFVTTHSYGVEEGFLDEYGQSDRKLSPNPDTI
                     VGDVKRVRQQIEASAFHGLPLYVTEWSTSYNPRDRVHDSYISASWILFKLRAARGHAQ
                     AMSYWTYSDLFEEPGPPDRPFHGGFGLMTREGVRKPAWFAYKYLNALRGKEIPSNDDA
                     VLAAVDGRKTAVLAWDWQHREQPTSNSVFFG"
     gene            complement(1287..>2092)
                     /locus_tag="VFV25_13080"
     CDS             complement(1287..>2092)
                     /locus_tag="VFV25_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HEY1394300.1"
                     /translation="AKDERLTPYTTSTAHSAGWQWRIPLQHRTGNGHVYCNGFVSDDE
                     ASSTLLQNLDGAALDAPRQLRFTTGRRREAWVKNVVAIGLSSGFLEPLESTSIQLIMD
                     GVGRLIELFPDRDFQPGLAAEFNRRMARQYESIRDFIILHYKLTERGDSEFWRYCASM
                     PIPDTLQQQIELFRASGRVVIFDPDSFAEPSWISIMFGLGLAPQAYDPFVDLIEEDAL
                     RTHFTRLREAIANTVAAMPDHAHFIARHVKAGPVVQPGAREAKPAVATA"
BASE COUNT          327 a          665 c          753 g          347 t
ORIGIN      
        1 ttgccgaaga agacgctgtt gctggtgggc tgctcgcggt gctgccagtc ccaggcgagc
       61 acggcggtct tgcggccatc gaccgccgcc agcactgcgt cgtcattgct cgggatctcc
      121 ttaccgcgca gcgcgttgag gtacttgtag gcgaaccatg ccggcttgcg cacgccctcg
      181 cgagtcatca ggccgaagcc gccgtggaag ggccggtcgg gcgggccggg ctcctcgaac
      241 aggtcgctgt aggtccagta gctcatggcc tgcgcgtggc cccgcgcggc gcgcagcttg
      301 aacaggatcc acgaggcgct gatgtagctg tcgtgcacgc ggtcgcgcgg gttgtagctg
      361 gtgctccact cggtcacgta cagcggtagg ccgtggaagg ccgaggcctc gatctgctgc
      421 cgcacgcgct tgacgtcgcc gacgatagta tcgggattcg gcgagagctt gcggtcggac
      481 tggccgtact cgtcgaggaa gccttcctcc acgccgtagc tgtgcgtggt gacgaaatcc
      541 accggcgcgt cgtgcgcctt ggcataagcc agaaactccg gcacccaggc cgcgcccgcg
      601 gtggaggggc cgcccacgcg cagctgcggg tcgatggcct tgatcgcctt agccgtgcgc
      661 gcgtagagct cgaagtaggc ctgctgatcg gcgcgctccc agaagccgtc gaggttgggc
      721 tcgttccaca cctcgaaggg ccagcgccgc acttcctcca cgccatagcg ctcgcgcagg
      781 tggcgcacga aggcttccac cagcgcctgc caggcctcgg gcttgggatg cgaggtgttg
      841 cccttccagt agaagatggt ctgcttcgag ctggccatcg cctcgggcgt gaagccgagc
      901 tcgacgaagg gccggatgcc cttggcgagc agtgcgtcgt agagcttgtc gatgccgctg
      961 aagtcgaaga ccaggcggcc ctcggcctcg cgcaccgtgt gcagcacgtc gtggaagatg
     1021 gcgtggaagc ggacgtagcg gaagcccagc tcatcgacgg cggtcttcag ctgtgcctgg
     1081 ctgtcgtcgc ggatcagcgt gccggggaag tcggagccga ccgagagatc gaagaagcgg
     1141 tcgaccggcc ccgccgcgcg cgcgaggtcc agctgcacct gccggtatcc cgggccgccc
     1201 gcgccggcgg ccgcggcata gcacaggccg agcgccagcc ccaatgccct cctccaatga
     1261 agacgctgca tgctccgctg ctctttctac gcggtagcga ccgctggctt ggcctcgcgc
     1321 gcgccgggct gcacgacagg acctgccttc acgtggcggg ctatgaaatg ggcgtgatcc
     1381 ggcatggcgg ccaccgtgtt ggcgatggct tcgcgcaggc gggtgaagtg ggtacgcagg
     1441 gcatcctcct cgatcaggtc gacgaacggg tcataagcct gtggcgccag gccgaggccg
     1501 aacatgatgg agatccacga cggttcggca aagctgtcgg ggtcgaagat gaccacgcgg
     1561 ccgctggcgc ggaacaactc gatctgctgc tgcagcgtgt cggggatggg catggaggcg
     1621 caatagcgcc agaactcgga gtcgccgcgc tcggtgagct tgtagtgcag gatgatgaag
     1681 tcgcggatcg attcgtattg ccgcgccatc cggcggttga actcggccgc caggccgggc
     1741 tggaagtcgc ggtcggggaa cagctcgatc agccggccca cgccgtccat gatgagctgg
     1801 atgctcgtgg attcgagcgg ctccaggaag ccggaagaca ggccgatggc cacgacattc
     1861 ttcacccagg cctcgcgccg ccggccggtg gtgaagcgca gctggcgcgg cgcgtccagc
     1921 gccgcaccgt ccaggttctg cagcagggtg gatgaggcct cgtcgtcgga gacgaagccg
     1981 ttgcagtaca cgtggccgtt gccggtgcgg tgctgcaatg gaatgcgcca ctgccagcct
     2041 gccgagtgcg cggtggacgt ggtgtagggc gtgagccgct cgtccttggc gc
//
LOCUS       DASUCY010000662         5393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1139539, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000662 DASUCY010000000
VERSION     DASUCY010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 5393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5393
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139539"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VFV25_13085"
     CDS             complement(<1..576)
                     /locus_tag="VFV25_13085"
                     /inference="COORDINATES: protein motif:HMM:NF020653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclomaltodextrinase N-terminal
                     domain-containing protein"
                     /protein_id="HEY1394301.1"
                     /translation="MIARLVTGIVLGLCALAAQAQVERIDPPSWWVGMKSRQLQLMVH
                     GPHIADYQPELHYPGVRLSKVTRVESPNYLFVDLDIAPLAKPGRLSLDFRKGAERITV
                     PYELLLRAPHSAERRGFGNADVILNLMPDRFANGNPANDDVPGFADQANRADNDAGRH
                     GGDIQGIVDHLDHIAAMGYTMLWPTPLTESNQ"
     gene            complement(573..2096)
                     /gene="malQ"
                     /locus_tag="VFV25_13090"
     CDS             complement(573..2096)
                     /gene="malQ"
                     /locus_tag="VFV25_13090"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF011080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEY1394302.1"
                     /translation="MMNLQERRSGVLVHITSLPGPHGMGDFGPDAYRFVDWLAAAGQR
                     LWQLLPITPIGPGDSPYQGVSAFAGSQWMVALEPLVEKGWLDTPVLPEGGGFDAHHVH
                     YGQVLPWREAQLRKAAVGFKARASAEDRKAFEAWRAAESAWLDDYALFMAIRSPRNGQ
                     PWWEWPADLARRDPTALQATRAEHADEIAFWQFVQWNFDLQARALKDYANRRGVALMG
                     DLPIFVAHDSADCWARPDLYYLDDKFQTTVVAGVPPDPLGPLGQRWGNPLYRWDRMAA
                     ENYAWWTARVRRALSHADVFRIDHFRGFAGYYEIPASSPDAKQGRWLPGPGKALFDAI
                     EGALGKLPIVAEDLGFITEDVHALRDGCGFPGMKILQFGFGADGTHEFLPHNWNTACV
                     AYTGTHDNDTVRGWWDNASPRERAYAGSYLACGAHDVHWAMIRACCNSVAAMAVFPMQ
                     DVLGLPSAHRMNTPGVLGGNWGWRFSWDMVGSEAVRVLGLITAASGRGPFELLHLPS"
     gene            complement(2120..4195)
                     /gene="glgX"
                     /locus_tag="VFV25_13095"
     CDS             complement(2120..4195)
                     /gene="glgX"
                     /locus_tag="VFV25_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEY1394303.1"
                     /translation="MHLPETIEEGRPEPLGSLARDGGVNFTVASQHARAIELCVFDAG
                     GARELRRYALHGPYEGVFNGFLPGVGPGLVYGLRAHGPYEPEAGHRFNAHKLLLDPYA
                     REIVGRFSWMAEHHAYELGHPEGPRSFDARDNGVQALKARVAQPPREAAGYANAPRHA
                     PADVVLYEVHVKGFSMLHPGIPPELRGSYAALAHPAAIAHFKRLGLTTLSLLPVQYAI
                     DEPHLAEKGLHNYWGYNTLGFFCPDPRWSRHRDDPTAVSDEFREMVRVLHEHGLEVVL
                     DVVYNHTPEGNEFGPSLSFRGLDQKLWYRLVPDDLSRSENLSGCGNTLHAAHPRVTQF
                     VLDSLRYWVQEMGVDGFRFDLAPVLGRTHHGFDPCAAFFTALRQDPVLARVHLIAEPW
                     DAGHDGYQVGRFPGRFLEWNDKFRDAVRGYWLRTGGVGRGELARRFTASSDLFHHAQR
                     RPAASVNFVSVHDGYTLADVVSYSHKHNHANGEDNRDGRDGELSANFGVEGPTQDAAI
                     LSTRERVRRAMLATLLLAQGTPMLCAGDEIGNSQQGNNNAYCQDNPIGWLGWPHADEG
                     LAGFVAELLALRRAEPLLRHARWFVPAPGAEGDACVAWFAPGGWEMQAHDWHDAGSAA
                     FACQISAGGANPRRVLVVFNPEPTTIAFTLPAGPWSLALDSSDELRPGSVGASLHIPA
                     RALCVLRSA"
     gene            complement(4343..>5393)
                     /gene="glgB"
                     /locus_tag="VFV25_13100"
     CDS             complement(4343..>5393)
                     /gene="glgB"
                     /locus_tag="VFV25_13100"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HEY1394304.1"
                     /translation="GRNEVRNFLAASALYWLERHGVDGLRVDAVASMLYRDYSRPSGE
                     WIPNVNGGNHNLEAISLLRHISEVLGADAPGGITVAEESTTFPGVSAPTYAGGLGFHY
                     KWNMGWMNDTLAYMREDPVHRRWHHGKLTFGLSYAFSENYVLPLSHDEVVHGKGSLLG
                     KMQGDGWQRYANLRAYYGFMWGHPGKKLLFMGQEFAQDREWNHDAELPWPLLQDPRHA
                     GVQCLVRDLNRLYRTQPALHRLDCEAAGFEWLVADDAEQSVFAWVRRDGAGGELIIVA
                     NFTPLPREDYRLGVPEGAAAWREALNTDSRHYGGSDVGNGAGALRVEPVADRGRAGSI
                     RLTLPPLATIFLVRA"
BASE COUNT          840 a         1810 c         1920 g          823 t
ORIGIN      
        1 ctgattgctc tcggtcagcg gtgtgggcca aagcatcgta tagcccatcg cggcgatgtg
       61 gtcgaggtga tcgacgatgc cctggatgtc gccgccgtgg cggccggcgt cgttgtcggc
      121 gcgattggcc tggtcggcaa agcccggcac gtcgtcattg gctggattgc cgttggcgaa
      181 gcggtccggc atcaggttga ggatcacgtc tgcattgccg aagccgcggc gctcggccga
      241 gtgcggcgcc cttagcagca gctcgtaggg cacggtgatg cgctcggcgc ccttgcggaa
      301 gtccagcgac agccggcccg gcttggccag cggcgcgatg tcgagatcga caaacagata
      361 gttcgggctc tcgacgcgcg tcaccttgct caggcgcacg cccgggtagt gcagctcggg
      421 ctggtagtcg gcgatatgcg gcccgtgcac catcagctgc agctggcggc tcttcatgcc
      481 cacccaccac gagggcggat cgatgcgctc cacctgggct tgcgcggcca acgcgcacag
      541 gcctaaaacg atgccggtca cgaggcgcgc gatcatgatg gaaggtgcag cagctcgaag
      601 ggaccgcggc cgctggccgc cgtgatcagc cccagcacgc gcacagcctc gctgcccacc
      661 atgtcccagc tgaagcgcca gccccagttg ccgccgagca cgccgggcgt gttcatgcgg
      721 tgggcgctgg gcaggcccag cacgtcctgc atcgggaaca cggccatcgc cgccaccgag
      781 ttgcagcagg cgcggatcat ggcccagtgc acgtcgtgcg cgccgcaggc caggtagctg
      841 ccggcgtagg cgcgctcgcg cgggcttgcg ttgtcccacc agccgcgcac ggtgtcgttg
      901 tcgtgggtgc cggtgtaggc cacgcaggcg gtattccagt tgtgcggcag gaactcgtgc
      961 gtgccgtcgg cgccgaagcc gaattgcagg atcttcatgc cggggaagcc gcagccgtcg
     1021 cgcagcgcgt gcacgtcctc ggtgatgaag cccaggtctt cggcaacgat gggcagcttg
     1081 cccaaggcgc cctcgatggc atcgaacagc gccttgcccg ggccgggcaa ccagcggccc
     1141 tgcttcgcgt cggggctgct cgcggggatc tcgtaatagc cggcgaagcc gcggaagtgg
     1201 tcgatgcgga acacgtcggc atggctcagt gcgcgccgca cgcgcgccgt ccaccaggcg
     1261 tagttctcgg cggccatgcg gtcccagcgg tacagcgggt tgccccagcg ctggccgagc
     1321 gggcccagag ggtcgggcgg cacgccggcg accaccgtgg tctggaactt gtcgtcgagg
     1381 tagtacagat cagggcgcgc ccagcagtcg gcgctgtcgt gcgcgacgaa gatcggcagg
     1441 tcgcccatca gcgccacgcc gcggcggttg gcgtagtcct tcagcgcgcg cgcctgcagg
     1501 tcgaagttcc attgcacgaa ctgccagaag gcgatctcgt cggcatgctc ggcgcgcgtg
     1561 gcctgcagcg cggtgggatc gcggcgggcc aggtccgccg gccattccca ccagggctgg
     1621 ccattgcgcg gcgagcgtat cgccatgaag agcgcatagt cgtcgagcca ggcgctctcg
     1681 gccgcgcgcc aggcctcgaa ggccttgcga tcttcggcgc tggcgcgggc cttgaagccc
     1741 acggccgcct tgcgcagctg ggcctcgcgc cagggcagca cctggccgta gtgcacgtgg
     1801 tgggcatcga aaccgccgcc ttcgggcagc accggggtgt ccagccagcc tttctcgacc
     1861 agcggctcca gcgccaccat ccactggctg ccggcgaagg ccgacacgcc ctggtagggc
     1921 gaatcgcccg ggccaatggg cgtgatgggc agcagctgcc acaggcgctg gccggcggcc
     1981 gccagccagt cgacgaagcg ataggcgtcg gggccgaagt cgcccatgcc gtgcgggccg
     2041 ggcagcgagg tgatgtgcac cagcacaccg ctgcgtcttt cctggagatt catcatgatt
     2101 gtcttttcgc tgaaatgatt caggcgctgc ggaggacgca cagcgcgcgc gccgggatat
     2161 gaaggctcgc gcccaccgag ccaggccgca gttcgtcgct ggaatccagc gccagagacc
     2221 aggggccggc gggcaacgtg aaggcgatag tcgtaggctc ggggttgaag accaccagca
     2281 ctctgcgcgg gttcgccccg ccggcgctga tctggcaggc gaaagccgcc gagcctgcat
     2341 cgtgccaatc gtgcgcctgc atctcccagc cgcccggcgc gaaccaggcg acgcaggcat
     2401 cgccctcggc gcccggcgcc ggcacgaacc agcgcgcgtg gcgcagcagc ggctcggcgc
     2461 ggcgcagcgc aagcagctcg gcgacgaagc cggcgagacc ctcgtcggca tgcggccagc
     2521 ccagccagcc gatggggttg tcctggcaat aggcgttgtt gttgccctgc tggctgttgc
     2581 cgatctcgtc gcccgcacac agcatgggtg tgccttgcgc gagcagcagc gtggccagca
     2641 tcgcgcggcg cacgcgctcg cgcgtggaga ggatggcggc atcctgcgtc gggccttcga
     2701 cgccgaagtt ggcactgagc tcgccgtcgc gtccgtcgcg gttgtcctcg ccgttggcgt
     2761 ggttgtgctt gtggctgtag ctcaccacgt cggccagcgt gtagccgtcg tgcaccgaga
     2821 cgaagttgac gctggccgcg ggccggcgct gcgcgtgatg gaagagatcg ctggacgccg
     2881 taaagcgccg cgccagctcg cccctcccga caccgccggt gcgcaaccaa tagccgcgca
     2941 ccgcgtcgcg gaacttgtcg ttccattcga ggaatcggcc cgggaagcgg ccgacctggt
     3001 aaccgtcgtg gccggcgtcc cagggttcgg cgatgaggtg cacgcgcgcg agcacgggat
     3061 cctggcgcag agccgtgaag aaggccgcgc aggggtcgaa gccatggtgg gtgcggccca
     3121 gcaccggcgc gaggtcgaag cggaagccgt ccacgcccat ttcctgtacc cagtagcgca
     3181 gcgagtccag cacgaactgc gtgacgcgcg ggtgcgcagc gtgcagcgtg ttgccgcagc
     3241 cgctgaggtt ctcgctgcgg ctcaagtcgt ccggcaccag ccggtaccag agcttctggt
     3301 cgagaccgcg gaagctcagg gaagggccga attcgttgcc ctcgggcgtg tggttgtaga
     3361 ccacgtcgag cacgacttcc aggccatgct cgtgcagcac gcgcaccatc tcgcggaact
     3421 catcggacac ggcagtaggg tcgtcacggt gacgcgacca gcgcgggtcc gggcagaaga
     3481 agccgagcgt gttgtagccc cagtagttgt gcaggccctt ctcggcgagg tgaggctcgt
     3541 cgattgcgta ctgcaccggc agcagcgaca gcgtggtcaa gcccaggcgc ttgaagtgcg
     3601 cgatcgccgc cgggtgcgca agcgccgcat agctaccgcg cagctcgggc gggatgccgg
     3661 gatgcagcat cgagaagccc ttgacgtgca cctcgtagag caccacgtcg gccggcgcgt
     3721 ggcgcggcgc gtttgcatag cccgccgctt cgcgcggcgg ctgggcgacc ctcgccttca
     3781 gtgcctggac gccgttgtcg cgcgcgtcga aggagcgcgg cccctccgga tggccgagct
     3841 cgtaggcatg atgctcggcc atccagctga agcggccgac gatctcgcgc gcataggggt
     3901 cgagcagcaa cttgtgcgcg ttgaagcgat ggcccgcctc gggctcgtag gggccgtgcg
     3961 cgcgcaggcc gtaaacaagg cccgggccga cgccgggcag gaagccattg aacacacctt
     4021 cataggggcc gtgcagggca tagcgcctga gctcgcgcgc gccgcccgcg tcgaacacgc
     4081 acagctcgat cgcgcgcgca tgctgcgagg ccacggtgaa gttgacgcca ccatcgcggg
     4141 ccagcgaacc cagcggctcg gggcgtcctt cctcgatagt ctctggcaaa tgcatggcgg
     4201 cctttagtca gttgcgcttc tcgcgcccag cgatggcatg accagagcct tgccaagcga
     4261 acgccgcgga tccggctccg ccggtccgct ggcgtcgccc ccttgagggg gtgcgccgaa
     4321 ggcgctacgg gggtgggtca tttcaggccc gaacgagaaa gattgtcgca agcggcggca
     4381 aggtcaagcg tatcgaaccg gcacggccgc gatcggccac cggctccacg cgcagcgcgc
     4441 ccgcgccgtt gccgacgtcg ctgccgccat agtggcgcga atcggtgttc agcgcctcgc
     4501 gccacgcggc cgcgccctcg ggcacgccca ggcgatagtc ctcacgcggc agcggcgtga
     4561 agttggcgac gatgatgagc tcgccgcccg cgccatcgcg ccgcacccag gcgaagaccg
     4621 actgctcggc gtcgtcggcc accagccact cgaagccggc ggcttcgcag tcgaggcggt
     4681 gcaacgcggg ctgggtgcga tagagccggt tgaggtcgcg caccaggcat tgcacgccgg
     4741 cgtggcgcgg atcctgcagc agcggccagg gtagctcggc gtcgtggttc cactcgcggt
     4801 cctgcgcgaa ttcctggccc atgaagagca gcttcttgcc cggatgcccc cacatgaagc
     4861 cgtaataggc gcgcaggttg gcgtagcgct gccagccgtc gccctgcatc ttgccgagca
     4921 gcgagccctt gccgtgcacc acttcatcgt gcgacagcgg cagcacgtag ttctcgctga
     4981 atgcgtagct caggccgaag gtgagcttgc cgtgatgcca gcggcggtgc accgggtctt
     5041 cgcgcatata ggccagcgtg tcgttcatcc agcccatgtt ccacttgtag tggaagccca
     5101 ggccgccggc ataggtgggc gccgacacac cgggaaaggt cgtcgactcc tcggccacgg
     5161 tgatgccgcc cggagcatca gcgcccagca cctcgctgat atggcgcagc agcgagatgg
     5221 cctcgaggtt gtggttgccg ccgttgacgt tgggaatcca ttcgccggaa ggccgggaat
     5281 agtcgcggta gagcatcgag gccacggcat cgacgcgcag gccgtccacg ccgtggcgct
     5341 ctagccagta caaggcgctg gccgcgagga agttgcgcac ctcgttgcgg ccg
//
LOCUS       DASUCY010000663         3023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_464898, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000663 DASUCY010000000
VERSION     DASUCY010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464898"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="VFV25_13105"
     CDS             <1..1009
                     /locus_tag="VFV25_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEY1394305.1"
                     /translation="VEDATAVVDDDSAFTPVEVQVLEAFIAQHYLDGNIPPLLVTSHA
                     VDAALIEALSEQAGVKLSTVHQPREQRRAWLEMAIKGCELALARLLAEEGSQQARTGA
                     LAEALDLPAENLDTLRIECFDISHTGGEATQASCVIFEHHVMQSSQYRRYNIEGITPG
                     DDYAAMRQVLTRRYAKLAEAAQRGEARLPDLVLVDGGRGQVSMAREVFEELGLDLSLI
                     VGVEKGEGRKVGLEELVFADGRSKVYLGRDSAALMLVAQIRDEAHRFAITGMRARRAS
                     VRTGGSRLEDIAGVGPKKRARLLQRFGGLRGVAAASVEDLAHVDGISRELAEEIYRAL
                     H"
     gene            1176..1484
                     /locus_tag="VFV25_13110"
     CDS             1176..1484
                     /locus_tag="VFV25_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1394306.1"
                     /translation="MRLQAARRLVTANPGLTYVDQPPDILLAIPVLEVELNGDGSIRR
                     INVMRYPSQAREKTQVAIDAMRRAGPFGSVAHLPKPWRFVETFLFRDDGKFKPMTLDR
                     "
     gene            1550..2116
                     /gene="pgsA"
                     /locus_tag="VFV25_13115"
     CDS             1550..2116
                     /gene="pgsA"
                     /locus_tag="VFV25_13115"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEY1394307.1"
                     /translation="MFLTLPTLLTWARIVAIPLIVGVFYLPLEGPTRNLIATVLFIVV
                     ALTDWADGFLARRLNQTSAFGAFLDPVADKFLVCASLLVLVQLDRVSALVALVIIGRE
                     IAISALREWMAQIGASRSVAVHMLGKLKTTVQMIAIPFLLFDGWLFGLLDTKRWGTAL
                     IYLAAVLTIWSMVYYLRKALPEIRAKVR"
     gene            2339..2611
                     /locus_tag="VFV25_13120"
     CDS             2339..2611
                     /locus_tag="VFV25_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEY1394308.1"
                     /translation="MNKTELIEHIAKQADISKAAATRALEALIGGVKTTLKKGNSVSL
                     VGFGTFAVTKRAARSGRNPRTGATIKIKAAKVPKFRPGKALKDAVN"
     gene            2624..2699
                     /locus_tag="VFV25_13125"
     tRNA            2624..2699
                     /locus_tag="VFV25_13125"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2657..2659,aa:Val,seq:tac)
     gene            2908..>3023
                     /locus_tag="VFV25_13130"
     CDS             2908..>3023
                     /locus_tag="VFV25_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEY1394309.1"
                     /translation="MFDFVRQHNRILQFVLVLLIFPSFVFFGIQGYTRFREG"
BASE COUNT          531 a          990 c          972 g          530 t
ORIGIN      
        1 cgtcgaggac gccacggcag tcgttgacga cgacagcgcg ttcacgcctg tcgaggtcca
       61 agtgctcgag gctttcatcg cccagcacta tctcgacggc aacattcctc cgctgctggt
      121 gaccagccat gcggtcgatg cggcgctgat cgaggcgctg tccgagcagg cgggcgtgaa
      181 gctcagcacc gtgcatcagc cgcgcgagca acgccgggcc tggctggaga tggcgatcaa
      241 gggctgcgag ctggcgctgg cgcgattgct ggccgaggag ggctcgcagc aggcccgcac
      301 cggcgcgctg gcagaggccc tcgacttgcc ggccgagaac ctcgatacct tgcgtatcga
      361 gtgtttcgac atcagccaca ccgggggcga ggcgacccag gcatcctgtg tgatattcga
      421 gcaccatgtc atgcagagct cgcaatatcg tcgctacaac atcgaaggca tcacgcccgg
      481 cgatgactac gccgcgatgc ggcaagtgct gacgcgccgc tacgccaagc tcgccgaagc
      541 ggcgcagcgc ggcgaagccc ggctccccga tctggtgctg gtggacggcg gccggggtca
      601 ggtcagcatg gcgcgagagg tcttcgaaga gctgggcctg gacctgagcc tgatcgttgg
      661 cgtcgagaaa ggcgagggcc gcaaggtcgg tctcgaagaa cttgtgttcg ccgatgggcg
      721 cagcaaggtc tacctgggtc gcgactcggc ggcgttgatg ctggtcgcgc agatccgcga
      781 cgaggcccat cgcttcgcca tcaccggcat gcgggccagg cgcgccagcg tgcgcaccgg
      841 cggtagcagg ctggaggaca tcgccggcgt ggggccgaag aagcgtgcgc gtcttctgca
      901 acgcttcggc gggctgcgcg gcgtggctgc ggccagcgtg gaagatctgg cacatgtgga
      961 cggcatttca agagaattgg ctgaggagat ttatcgtgcg cttcactgaa cctcgcattg
     1021 cgttggcctt gagcctgttg ctcgcggctt gcgccgggcc gcagcccgcg ccgcggacta
     1081 gcccggctgc gccgcagccg cggcccagca gttcaacgcc accaccgtcg gcttcggggc
     1141 taccggctcc ctcggcagcg cgcaattggg atgaggtgcg cctgcaggcc gcgcggcgcc
     1201 tggtgacggc caatccgggt ctgacctatg tcgaccagcc gcccgacatc ctgctggcta
     1261 ttcccgtgct cgaggtcgaa ctcaacggcg atggcagcat ccgtcgcatc aatgtgatgc
     1321 gctaccccag ccaggcgcgc gagaagaccc aggtggcgat cgacgcgatg cggcgcgccg
     1381 gccccttcgg cagcgtcgcc catctgccca agccctggag gttcgtcgag accttcctgt
     1441 tcagggacga cggcaagttc aagccgatga cgctggacag gtgagaacag gcgggacggg
     1501 cgcgcctaca gcagggcgcg gcgacgcgca agtccggcac aatggggcga tgtttctcac
     1561 cctgcccacg ctgctgacct gggcgcgcat cgtcgcgata cctctcatcg tcggcgtctt
     1621 ctatcttccg ctcgaagggc cgacccgcaa cctcatcgcc accgtgctct tcatcgtcgt
     1681 ggcgctcacg gattgggccg acggattcct ggcacgcaga ctcaatcaga cctcggcctt
     1741 cggcgccttc ctcgacccgg tggctgacaa gttcctggtc tgcgcctcgc tgctggtgct
     1801 ggtgcagctc gatcgcgtca gcgcgctggt ggccctggtc atcatcggac gcgagatcgc
     1861 catctcggcg ctgcgcgaat ggatggccca gattggtgct tcgcgcagcg tggccgtgca
     1921 catgttgggc aagctcaaga ccacggtgca gatgattgcc attcccttcc tgctgttcga
     1981 tggctggctg ttcggcctgc tggacaccaa gcgctggggc acggcgctga tctatctggc
     2041 cgccgtgctc accatctggt cgatggtgta ctacctgcgc aaagccttgc ccgagatacg
     2101 ggccaaggtg cgctgaagcg gccggtgcat gccggtcatg cattgcatca agcgcggccc
     2161 aatgcttgca gcgctctggc cgcgagatga gcacgattac acatagaatc ccgaaccgcg
     2221 cttgacactg aagagtcgca cggctgtaat caagcgctcg aagcggactg catccctgca
     2281 ccactcatcg catcgaaccc gaacattcct tgagacacct ttgagagggg gtacctacgt
     2341 gaacaagacc gaattgattg agcacatcgc caagcaggcc gacatctcca aggctgctgc
     2401 cacgcgcgcg ctggaggccc tgatcggcgg tgtgaagacc acgctcaaga aaggcaacag
     2461 cgtttcgctg gtcggtttcg gcaccttcgc cgtcaccaag cgtgccgctc gcagcggccg
     2521 caatccgcgc accggcgcca ccatcaagat caaggccgcc aaggtgccca agttccgccc
     2581 cggcaaggca ttgaaggacg cggtcaactg aagcaagctc gttgggtgct tagctcagtt
     2641 ggtagagcgg cgcccttaca aggcgtaggt cgggggttcg aacccctcag cacccaccag
     2701 acccgcagcg cgcttcggcc caggcgctgc actcccggcg agtatcattg cggtctagcg
     2761 cgccggggat agcaaacagg ctcccgctgg acgaccagcg tagagcatcc cccaagcaaa
     2821 ggcgaacccc ggttcgcctt tgtcgcttcc agcgaagacc tcttcgccgg gcaccggtcg
     2881 caagcgactc atcctcgagg ctcattgatg ttcgatttcg tccgccagca caaccgcatc
     2941 ctgcaattcg tcctggtgct gttgatcttc ccgtccttcg tcttcttcgg catccagggc
     3001 tacacaaggt tcagggaagg caa
//
LOCUS       DASUCY010000664         2021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1284039, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000664 DASUCY010000000
VERSION     DASUCY010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284039"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="VFV25_13135"
     CDS             <1..704
                     /locus_tag="VFV25_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEY1394310.1"
                     /translation="QIAHLRRGAVTDVVRDYVPADSVEEQWDLGGLEKALREEWQLEV
                     PLKAEIEKRDAVSDEDVVELAGKAADAAFESKLGQVGEQQFAAFERMILLQSIDTHWR
                     EHLAALDYLRQGIHLRGYAQKNPKQEYKREAFELFGQLLDTVKMDVTRILMTVRIQSQ
                     EEAAKAAEAIEEKVEAISNVTFTHPNEDGSVTSDPLHSEAGVPLAARVPRVGRNDPCP
                     CGSGKKYKHCHGRLA"
     gene            912..>2021
                     /locus_tag="VFV25_13140"
     CDS             912..>2021
                     /locus_tag="VFV25_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEY1394311.1"
                     /translation="MFDKRFFPRQVVESGGNRWDWALLPPVLLLVVLLAYGGSHMARP
                     YQVGDTLPLTLEPWVLPYYLLRTTMRMALAMVASLLFACVFATLAAKYRAAEKVLVPL
                     LDILQSIPILGFLSITVTGFIALFPGNLLGVECAAIFAIFTSQAWNMAFSLYQSLRTV
                     PTELQEAARVFQLSGWQRFWRLELPFAMPGLLWNMMMSMSGGWFFVVASEAISVSNQN
                     IKLPGVGSYIALAIEARDLGAIGWAIGGMLVGIILYDQLLFRPLVAWADKFRFEESGS
                     DTAPQSWLLTWLRRTDRISAMGDWATRQLERSFALFKRDYDGTSIRARDPEPNPTLAR
                     AWDAILAAVVLIAIWRLASFIHAEVGWGEVLHVFKL"
BASE COUNT          307 a          694 c          660 g          360 t
ORIGIN      
        1 cccagatcgc tcatctgcgc cgcggcgccg tcaccgatgt cgtgcgcgac tatgtgccgg
       61 ccgacagcgt cgaggagcaa tgggacttgg gcggtctcga gaaggcgctg cgcgaggaat
      121 ggcagctcga ggtgccgctc aaggccgaga tcgagaagcg cgatgccgtg agcgacgaag
      181 acgtcgtcga gctggccggc aaggctgccg acgcggcctt cgagtccaag ctcggccagg
      241 tcggcgaaca gcagttcgcc gccttcgagc gcatgatcct gctgcagtcg atcgacacgc
      301 attggcgcga gcacctggct gcgctggact acctgcgcca gggcatccat ctgcgcggct
      361 acgcccagaa gaaccccaag caggaataca agcgcgaggc cttcgagctc ttcggccagc
      421 tgctggacac ggtgaaaatg gacgtcacgc gcatcctgat gacggtgcgc atccagagcc
      481 aggaggaggc ggcgaaggcg gccgaggcga tcgaggagaa ggtcgaggcc atttccaacg
      541 tcaccttcac gcacccgaac gaggacggca gcgtcacctc cgatccgctg cacagcgagg
      601 ccggcgttcc gctcgccgcc cgcgtgcccc gcgtcggccg caacgaccca tgtccctgcg
      661 gcagcggcaa gaaatacaag cattgccacg gccggttggc ttgaccaccc cctacggcct
      721 tcaaggggcg acgccggtgg cccggcaaag ccggctccac ggcgtccggc ttggtcgcgc
      781 ttgcagtgct gacagcgtcg cttgaccgcc actgagggcg ggccggggcg ccgtcatcac
      841 attgccgccg ggtttggtga caatcgcgcg tttgacctcg ccgccgtaac gaccgccctt
      901 cctgccccgc catgttcgac aagcgcttct ttcctcgcca ggtggtcgag tccggcggta
      961 atcgttggga ctgggccttg ctgccgcccg tgctgctgct ggtcgtgctg ctggcctatg
     1021 gcggctcgca catggcgcgg ccctatcagg tgggcgacac actgccgctg acgctcgaac
     1081 cctgggtgct gccctactac ctgctgcgca cgacgatgcg catggcgctg gcgatggtcg
     1141 cctcgctgct gttcgcttgc gtctttgcga cgctcgccgc caagtaccgc gccgccgaga
     1201 aggtgctggt gccgctgctg gatatcctgc aatccatccc catcctcggt ttcctgtcga
     1261 tcaccgtcac cggcttcatc gcgctcttcc ccggcaacct gctgggcgtc gaatgcgcgg
     1321 cgatcttcgc gatcttcacc tcgcaggcct ggaacatggc cttcagcctc taccagtcct
     1381 tgcgcaccgt gccgaccgag ctgcaagagg cggcgcgcgt gttccagctc tcgggctggc
     1441 agcgcttctg gcggctggag ctgcctttcg cgatgcccgg cctcttgtgg aacatgatga
     1501 tgtcgatgtc cggcggctgg ttcttcgtgg tcgcttcgga agcgatctcg gtgtccaacc
     1561 agaacatcaa gctgcccggc gtcggctcct acatcgcgct ggccatcgag gcgcgcgacc
     1621 tcggcgccat cggctgggcg atcggcggca tgctggtggg catcatcctt tacgaccagc
     1681 tgctgttccg cccgctcgtc gcctgggccg acaagttccg tttcgaggag agtggcagcg
     1741 acacggcgcc gcaatcgtgg ctgctgacct ggctgcgccg cacggaccgc atcagcgcga
     1801 tgggcgactg ggccacgcgc cagctcgagc gttcgttcgc gctgttcaag cgcgactacg
     1861 acggcacctc gatacgtgcc cgtgatcccg agcccaaccc cacgctggcg cgcgcctggg
     1921 atgccatcct cgcggcggtc gtgctgatcg cgatctggcg cctggcgagc ttcatccatg
     1981 ccgaggtcgg ctggggcgaa gtgctgcacg tgttcaagct c
//
LOCUS       DASUCY010000665         4258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1059238, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000665 DASUCY010000000
VERSION     DASUCY010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4258
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059238"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="VFV25_13145"
     CDS             <1..706
                     /locus_tag="VFV25_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEY1394312.1"
                     /translation="SQGKKAILWAFNMSFFSWPIFASTGAYVFAKDAQGDFDPTRIGI
                     DTPGAARAGQLLSDMVNRDGLMPRMASYADMEAGFNHGDIAMMISGPWAWDNARRNHI
                     DFGVAPIPAIEPGHPGRPFVGVLGCMIAAPSQVKDIAREFIENHLMRPESLRLINADV
                     PLGVPASKAFYAELASDPNIRASMENARLGEPVPNIPETGRFWTAMDAAIEAITNGLQ
                     SPADALNGAAARILVK"
     gene            703..2271
                     /gene="malF"
                     /locus_tag="VFV25_13150"
     CDS             703..2271
                     /gene="malF"
                     /locus_tag="VFV25_13150"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HEY1394313.1"
                     /translation="MKQPAAQTSGLGGLLKWPLSIALALVFLYLVFKLFVAGQAIWAM
                     GLLALASLGFYVYLSNAAFAFRYLFPGLAGVAIFVAFPMLYTAQIGFTNYSSAHLLSE
                     ARARGYLLEQTQPDEARLMNYTLHADGKEFRLVLEGADAGAATAAATRYISPRLALLS
                     ANADAKIAMQPLETTEGLGLGAPLPLAEVLKHRDVLMRLTLELPDHSVLNYAGVREFA
                     PLKPLWKTNGDGSLKQLETGALYRANRDSGYFEDASGEHLQPGFKVGVGFANYARMVF
                     DPDFRGPFLSIFVWTVAFSALTVLFTLILGMSLAVLLNWEALPYRTVYRTLLFLPYAV
                     PSFISILVFKGLFNQNFGEINAILDAVFGTRPAWFADPLLAKVMLLIVNTWLGYPYIM
                     VLCSGLLKALPSDLYEASAIAGASPWTNFTKITAPLIIKPLTPLLISVFAYNFNNLVL
                     ITLLTKGRPDFLNTKVPAGTSDILVSYTYRIAFQDSGQNFGLAAAISTLIFLMVAALS
                     LANLRLARVNQAGK"
     gene            2284..3174
                     /gene="malG"
                     /locus_tag="VFV25_13155"
     CDS             2284..3174
                     /gene="malG"
                     /locus_tag="VFV25_13155"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HEY1394314.1"
                     /translation="MAIVTGKNQRWRLLAAHAFLWVLIALTLFPLLAVVSISLRPGNL
                     STGALIPEHISLEHWKLALGIPYQAADGSLVQPPFPVLLWLWNSIKIATISALLIVAI
                     STTAAYAFARLKFRFKSTILNSMLLLQMFPAVLALVAIYAIFETIGNYVPWLGVETHA
                     GVILAYLGGVAMHIWTIRGYFETIPAEIEECAKVDGATHWQAFVRVLLPMAVPILMVV
                     FVLAFVGTIIEYPVASVLLREEPNLTLAVGSKYYLNDQKFLWGDFAAAAVLSGLPITA
                     VFLAAQRWIVSGLTAGGVKG"
     gene            3285..>4258
                     /locus_tag="VFV25_13160"
     CDS             3285..>4258
                     /locus_tag="VFV25_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1394315.1"
                     /translation="MTSEIPPHCSAAPSLGSGDATLYLRGTMNNWTALDDYEFQYSCD
                     AYYLNVQLQGPQEFKIADAAWGDATTFGAARRDAATPALDQPLALGRGNGPGGATNLN
                     FAFDGEQTIRLAFVQGKPLVSIGPKRFVDPSHKPVTDPVALSLRFDSRRLADKSPFGA
                     VPAGTAVDFALEAAPGVTGVTLVIEKRRVEGNQDLLEYTEVARVPLRKHRQQGSVERW
                     SARHRFNDVSVYGYYFEAQIAGKTYVYENNRDTVHWTREKGSNGLGLVEEKPLSNKRI
                     RRYRHTVYAADFRVPAWARDVVYYYIFPDRFRNGDLSNDPRPGVDRYQ"
BASE COUNT          689 a         1498 c         1311 g          760 t
ORIGIN      
        1 gtcccagggc aagaaggcca tcctctgggc tttcaacatg tccttcttca gctggcccat
       61 cttcgccagc accggcgcct atgtgttcgc gaaggacgcc cagggcgact tcgatccgac
      121 caggatcggc atcgacaccc caggcgccgc acgcgccggc cagctgctga gcgacatggt
      181 gaaccgcgat ggcctgatgc cgcgcatggc gagctatgcc gacatggagg ccggcttcaa
      241 tcacggcgac atcgcgatga tgatctccgg cccctgggcc tgggacaacg cgcgccgcaa
      301 ccacatcgac ttcggcgtcg cgcccatccc ggccatcgag cccggccatc cgggccgccc
      361 cttcgtcggc gtgctgggct gcatgatcgc cgcgccgagc caggtcaagg acatcgcccg
      421 cgagttcatc gagaaccatc tgatgcgccc cgagagcctg cggctgatca atgccgacgt
      481 gccgctgggc gtgccggcca gcaaggcctt ctatgccgag ctcgcctcgg acccgaacat
      541 ccgcgcctcg atggaaaacg cccgcctcgg cgaaccggtg cccaacatcc cagaaaccgg
      601 ccgtttctgg accgcgatgg acgcagccat cgaggccatc accaacggcc tgcaatcccc
      661 tgccgacgcg ctgaacggcg ccgcggcacg catcctcgtc aaatgaagca gcctgcagca
      721 caaacaagcg gtctaggcgg cttgctcaaa tggccgctga gcatcgcgct ggccctggtc
      781 ttcctctacc tggtgttcaa gctcttcgtg gcgggccagg cgatctgggc gatgggcctc
      841 ctggcgctgg cctcgctggg cttctacgtc tatctgtcga acgcggcctt cgccttccgc
      901 tacctgttcc cggggctggc cggcgtggcg atcttcgtcg ccttcccgat gctctacacg
      961 gctcagatcg gcttcacgaa ctattcgtcg gcccatctgc tgagcgaagc tcgcgcccga
     1021 ggctatctgc tggagcagac ccagcccgac gaggcgcggc tgatgaacta cacgctgcac
     1081 gccgacggca aggagttccg gctcgtgctg gagggtgctg atgcgggcgc ggccactgca
     1141 gcggccacgc gctatatctc gccgcggctg gcgctgctgt ctgccaatgc cgatgcgaag
     1201 atcgcgatgc agccgctcga gaccaccgaa ggcctggggc ttggcgcgcc gctgccgctc
     1261 gccgaggtgc tcaagcatcg cgacgtgctg atgcggctca cgctggagct gcccgaccac
     1321 agcgtgctga actacgccgg cgtgcgcgag ttcgcgcccc tcaaacccct gtggaagacc
     1381 aacggcgacg gctcgctgaa gcagctcgaa accggcgcgc tctatcgcgc caaccgcgac
     1441 agtggctatt tcgaggacgc gagcggcgag cacctgcagc ccggcttcaa ggtcggcgtg
     1501 ggtttcgcga actatgcccg catggtcttc gacccggact tccgcggccc cttcctgtcc
     1561 atcttcgtgt ggaccgtcgc cttctcggcg ctcacggtgc tgttcacgct gatcctgggc
     1621 atgtcgctcg cggtgctgtt gaactgggag gcgctgccct accgcaccgt ctaccgcacc
     1681 ctgctctttt tgccctatgc ggtgccgtcc ttcatctcca tcctggtgtt caagggcttg
     1741 ttcaaccaga acttcggcga gatcaacgcg atcctggatg cggtcttcgg cacccgcccg
     1801 gcctggttcg ccgatccttt gctggccaag gtgatgctgc tgatcgtcaa cacctggctg
     1861 ggctacccct acatcatggt gctgtgcagc ggcctgctga aggcactgcc ctcggatctc
     1921 tacgaggcct cggccatcgc cggcgcctcg ccgtggacca atttcaccaa gatcacggcg
     1981 cccctgatca tcaagccgct gactccgctg ctgatctcgg tgttcgccta caacttcaac
     2041 aacctggtgc tgatcacgct gctgaccaag ggccggcccg acttcctcaa caccaaggtc
     2101 ccggcgggca cctcagacat cctggtgtcc tacacctatc gcatcgcctt ccaggactcg
     2161 ggccagaact tcggcctggc cgcggcgatc tcgacgctga tcttcctgat ggtggccgcg
     2221 ctgtcgctgg ccaacctgag gttggcgcgc gtcaaccagg caggcaaata aagagaaccg
     2281 cacatggcca tcgtgaccgg caagaaccag cgctggcgcc tgctcgcggc gcacgccttc
     2341 ctgtgggtgc tcatcgcgct cacgctgttc ccgctgctcg cggtggtgtc gatttcgctg
     2401 cgaccgggca atctgtccac cggcgcgctg attcccgagc acatcagcct ggagcattgg
     2461 aagctggccc tgggcatccc ctaccaggcc gccgacggca gcctggtgca gccgcccttc
     2521 ccggtgctgc tgtggctgtg gaactcgatc aagatcgcga ccatttcggc gctgctgatc
     2581 gtcgccatct cgaccaccgc ggcctatgcc ttcgcgcggc tgaagttccg cttcaagtcg
     2641 accatcctca acagcatgct gctgctgcag atgtttccgg ccgtgctggc gctggtggcg
     2701 atctacgcga tcttcgagac catcggcaac tatgtgccct ggctgggcgt cgagacgcac
     2761 gccggcgtga tcctggccta tctcggcggc gtcgcgatgc acatctggac catacgcggc
     2821 tatttcgaga ccatcccggc cgagatcgag gaatgcgcca aggtcgacgg ggccacccac
     2881 tggcaggcct tcgtgcgcgt gctgctgccc atggccgtgc ccatcctgat ggtggtcttc
     2941 gtgctggcct tcgtcggcac catcatcgaa tacccagtgg cctcggtgct gctgcgtgag
     3001 gagcccaatc tcacgctggc cgtgggttcc aagtactacc tgaacgacca gaagttcctg
     3061 tggggcgatt tcgccgcggc ggccgtgctc tcgggcctgc cgatcacggc cgtcttcctg
     3121 gccgcgcagc gctggatcgt ctcgggcctg accgccggcg gcgtcaaggg ttgaccgtgg
     3181 ggaatctgca tacgccctcg cattggctgc gggcaggcat gatggcgctt gcgctcgtag
     3241 tcagcgcgca cgccaaggat gccgccccga atcccccctc gaccatgacc agcgagatcc
     3301 cgccgcactg cagcgccgcg ccttctttag gttcaggcga tgcgacgctg tacctgcgcg
     3361 gcacgatgaa caactggact gcgctggacg actacgaatt ccagtacagc tgcgacgcct
     3421 actacctcaa cgtgcagctg caggggccgc aggaattcaa gatcgccgac gcggcctggg
     3481 gtgatgcgac caccttcggc gcagcgcgcc gggatgcggc gacgccggcg ctggatcagc
     3541 cgctcgcgct cggccggggc aatggccccg gcggcgcgac caatctgaat ttcgccttcg
     3601 acggcgagca gacgatccgc ctggccttcg tgcagggcaa gcctttggtc agcatcggcc
     3661 ccaagcgttt cgtcgacccg agccacaagc cggtgaccga ccccgtcgcc ttgagcctgc
     3721 gcttcgactc gcgccggctg gccgacaagt cgcccttcgg cgccgtgccg gccggcacag
     3781 ccgtggattt cgcgctggaa gccgcgcccg gcgtgacggg cgtgacgctg gtgatcgaga
     3841 agcggcgcgt cgagggcaac caggatctgc tggagtacac cgaggtcgca cgcgtgccgc
     3901 tgcgcaagca caggcaacaa ggcagcgtcg agcgctggag cgcgcgccac cgcttcaacg
     3961 acgtcagcgt ctacggctac tacttcgagg cgcagatcgc gggcaaaacc tatgtctacg
     4021 agaacaaccg cgacacggtg cactggacgc gcgagaaagg ctccaacggc ctgggcctgg
     4081 tcgaggagaa gccgctgtcg aacaagcgca tccgccgcta tcgccacacg gtgtacgcgg
     4141 ccgatttccg cgtgccggcc tgggcccgtg atgtcgtcta ctactacatc ttccccgacc
     4201 gctttcgcaa cggcgacctc agcaacgacc ccaggcccgg cgtcgaccgc taccagga
//
LOCUS       DASUCY010000666         3374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1155850, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000666 DASUCY010000000
VERSION     DASUCY010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155850"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VFV25_13165"
     CDS             complement(<1..216)
                     /locus_tag="VFV25_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HEY1394316.1"
                     /translation="MTKTASASKAGATKGTPAYQLYVELDWVRPKVWRRLLVPVTIEL
                     PLLHVMLLWGMGWEGGHLHEFVFGPDHY"
     gene            complement(264..1874)
                     /locus_tag="VFV25_13170"
     CDS             complement(264..1874)
                     /locus_tag="VFV25_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011402070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEY1394317.1"
                     /translation="MTLDEALHRNAELGAQVQRFEEELKVAQLTIEKLKVEVAYLRRM
                     KYGRSSERLEHAQLELVGGQVTPTVETETAVADTSNVASLDEHRKKRARRPRPNLRDL
                     PDHLPRRTVMHLPAGHQAGCGCPACGLALREIGQDVSEVLDYEPGSFHVVRHVRPKLA
                     CAGCKDVKQAAAPNRPIERVMAGAGMLAHVLVSKYCDHTPLYRQTQIYARAGLELHRS
                     TLTDWVAQAAHLLTPLAEAIGRYVLAAYKVHGDDTPIRALGGKGGKVHTGRLWVYVRD
                     DRPSGSKAAPAVWFQYSVDRKGDHPAKHLKRFSGVLQADAFAGYNALYNEDREGGRII
                     EAGCWSHARRKLWDIHVRQHKLAGTLAHQALERMGKLFKIEAEIHGKPPDDRRRVRRE
                     STAPLLEDLKAWMNATLTRVSAKSPMALAIGYALSNWTALTCFVDDGRIEAHNNAAER
                     ALRGVALGRKNYLHLGSDVGGQSAAVIYTLIGSAKLNGIDPQRYLTYVLERIAEHSIN
                     RIDELLPWVVASKLRAPWDAGVETAKAA"
     gene            complement(1959..2306)
                     /gene="tnpB"
                     /locus_tag="VFV25_13175"
     CDS             complement(1959..2306)
                     /gene="tnpB"
                     /locus_tag="VFV25_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536669.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEY1394318.1"
                     /translation="MIGPHAGTRVWLAAGVTDMRCGIDSLAAKVQTVLAEDPYAGHVF
                     AFRGRRGDLVKLLWSDGDGMCLLTKRLERGRFVWPQADSGSVALSVAQLSMLLEGIDW
                     RRPERTWQPRAAA"
     gene            complement(2303..2716)
                     /locus_tag="VFV25_13180"
     CDS             complement(2303..2716)
                     /locus_tag="VFV25_13180"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEY1394319.1"
                     /translation="MVHTMKSEQDPGKRSRRQHDRAFKEELVRQSLEPGASVSAIALR
                     NGINANMLFKWRREQRRAVAMREAPAVLLPVEVTPRAAVTTVPAPIATTTPTPEPPAR
                     NGVIEMDIAGVQIRVRGAVDEASLCSVLRALRQAR"
     gene            complement(2783..3160)
                     /locus_tag="VFV25_13185"
     CDS             complement(2783..3160)
                     /locus_tag="VFV25_13185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1394320.1"
                     /translation="MKATVSLAELMHAFDWVSAAGPFENEAYVSRDTGKIHWASDARE
                     LDEELPEDIEDGSLYVAVPHKSDLDLGRSLALRFVEEHMPESHGVVRGYFGRAGAYSR
                     FKDLLERADRLEACNSPTKAVLA"
BASE COUNT          506 a         1172 c         1101 g          595 t
ORIGIN      
        1 gtagtggtca ggaccgaaca cgaactcgtg caggtggccg ccctcccagc ccatgcccca
       61 cagcagcatc acgtgcagca gcggcagctc gatcgtcacg ggcacgagca agcggcgcca
      121 caccttgggt ctgacccagt cgagttcgac gtagagctgg tacgccggcg tgcctttcgt
      181 agcgccggcc ttcgatgctg aggcggtctt ggtcatcacc gcactttatc ggcagtgggg
      241 atgcgcgcgc agcgctgccg cagtcaggcg gccttggccg tttccacgcc ggcgtcccag
      301 ggcgcacgca gcttggacgc caccacccac ggcagcagct cgtcgatccg gttgatcgag
      361 tgctcggcaa tgcgctccag gacgtaggtc aggtagcgct gcgggtcgat gccgttgagc
      421 ttggccgagc cgatgagcgt gtaaatcacc gcggcgctct ggcccccgac gtccgacccg
      481 aggtgcaggt agttcttcct gccaagcgcg acgccgcgca gggcgcgctc ggccgcgttg
      541 ttatgtgcct cgatgcggcc atcgtcgacg aagcacgtga gcgccgtcca gttgctcagc
      601 gcgtacccaa tggccaacgc catcggcgac ttggccgaca cccgcgtcag cgtcgcattc
      661 atccacgcct tgaggtcttc gagcagcggc gccgtgctct ctcgcctgac ccgccttcgg
      721 tcgtcgggtg gcttgccatg gatctcggcc tcgatcttga acagcttgcc catgcgctcc
      781 agcgcctggt gcgccagcgt gccggccagc ttgtgctgtc gcacgtggat gtcccacagc
      841 ttgcggcgcg cgtggctcca gcatccggct tcgatgatgc gcccgccctc gcgatcctcg
      901 ttgtacaggg cgttgtagcc ggcgaacgcg tcggcctgca gcacgccgct gaagcgcttc
      961 aagtgcttgg caggatgatc gcccttgcgg tcgaccgagt attggaacca caccgcagga
     1021 gccgccttgc tgccactcgg gcggtcgtca cgaacgtaga cccacagccg gccggtatgc
     1081 accttgccgc ccttgccgcc gagcgctcgg atcggcgtgt cgtcgccgtg gaccttgtag
     1141 gccgcgagca cgtagcggcc gatggcctcg gccagtggcg tcaacaggtg cgccgcctgg
     1201 gccacccagt cggtcagcgt cgagcgatgc agttccaggc cggcacgcgc gtagatctgc
     1261 gtctggcggt acagcggcgt gtgatcgcag tatttgctga cgagcacgtg ggccagcatg
     1321 ccggcaccgg ccatcacgcg ctcgatcggc cggttcggcg ctgcggcctg cttgacgtcc
     1381 ttgcacccgg cgcacgccag cttcgggcgc acgtgccgca cgacgtggaa gctgccgggt
     1441 tcgtagtcga gcacctcgct gacgtcctgg ccaatctcgc gcagcgccag cccgcaggcg
     1501 gggcagccac agccggcttg atgccccgct ggcaagtgca tgacggtgcg ccgcggcagg
     1561 tggtccggca agtcacgcag gttcggccgc ggccggcgtg cccgcttctt gcgatgttca
     1621 tcgagcgacg cgacgttgct cgtgtcggca acggccgtct cggtctccac ggtcggcgtc
     1681 acctggccgc cgaccagctc gagttgcgcg tgctcgagtc gctcgctgga gcggccgtac
     1741 ttcatgcgtc gcaggtacgc gacctcgacc ttgagcttct cgatggtcag ctgcgcgacc
     1801 ttgagctctt cttcgaagcg ctgcacctgg gcgccgagtt cggcgttgcg gtgcagggct
     1861 tcgtccaagg tcatggagcg cgagtgtgcc gcgcgcgccg cagccttcac aacggggcat
     1921 gccccgtcaa cctgatggaa cgctcttgca gtgcagcctc aggccgcggc gcgcggctgc
     1981 caggtgcgct cgggcctgcg ccagtcgatg ccctccagca gcatcgacag ctgcgcgacg
     2041 ctcaacgcca cgctgccgct atcggcctgc ggccagacga agcggcctcg ctccaggcgc
     2101 ttggtcagca ggcacatgcc gtcgccgtcg ctccacagca gcttgaccag atcgcctcgg
     2161 cgcccgcgga aggcgaacac gtgaccggcg tacgggtcct cggccagcac ggtctgcacc
     2221 ttggccgcga gcgagtcgat gccgcagcgc atgtcggtga cgccggcggc cagccagacg
     2281 cgggtgccag cgtgcgggcc gatcatcgcg cttggcgcag cgcgcgcaag acgctgcaca
     2341 ggctggcctc gtcgaccgca ccgcgcaccc tgatctgcac gccggcgatg tccatctcga
     2401 tgacgccgtt gcgagccggc ggctcgggcg tcggcgtggt cgtcgcgatc ggcgcgggca
     2461 ctgtggtgac cgcggcccgc ggcgtcacct ccaccggcag caacacagcc ggcgcttcgc
     2521 gcattgcgac ggcgcgtcgc tgttcgcgcc gccacttgaa cagcatgttg gcgttgatgc
     2581 cgttgcgcag cgcaatcgcc gacacggatg cgccgggctc caagctctgc cgcaccagct
     2641 cttccttgaa cgcgcggtcg tgctgtcgcc gactccgctt cccagggtct tgctcactct
     2701 tcatggtgtg caccatctcc gtttagcgca cacctccaag cgggtgcgcg cgtcgggtca
     2761 gatgcttccc accggccgcg cgtcaggcaa ggacggcctt cgtcggacta ttacaggctt
     2821 caagccggtc ggcgcgttcc agcagatctt tgaagcggga gtacgcaccc gccctgccga
     2881 agtagccgcg tacgacaccg tgcgactccg gcatatgctc ctcgacaaac cgaagcgcaa
     2941 ggctacggcc aagatcaagg tcactcttgt gcgggacggc cacgtagagg ctaccgtctt
     3001 cgatgtcttc cggaagctcc tcgtcgagtt cgcgggcgtc cgacgcccag tgaatcttgc
     3061 cggtatctcg gcttacatag gcctcgttct cgaacgggcc tgcggcgctg acccagtcga
     3121 atgcatgcat cagttcagca agacttaccg ttgctttcat gggctagctc cgagctccaa
     3181 ggccgccagc ggacaacgca tgagcacgct gaggtagtcg gggcggagct gctcatcggg
     3241 ctgaccagcc aacgctaagg ttcaccggag gcatgccgca ggtgccggtt ccactcgatg
     3301 gagaggttag gttttcaccc ttccccgttg aagctgaact ccgagaacgc gccgctcttc
     3361 ggatcaaaca ctac
//
LOCUS       DASUCY010000667         3032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1235874, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000667 DASUCY010000000
VERSION     DASUCY010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235874"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VFV25_13190"
     CDS             complement(<1..327)
                     /locus_tag="VFV25_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1394321.1"
                     /translation="MREPATADRVEVLVLVDNATDNLSLVPSYVENELPRLWSRGLRL
                     WSGRCMCCAAHGLSCAVTAWRDGTAHTLLFDTGPDDSVFERNVERLGFDMGGVECIVL
                     SHGHWDH"
     gene            559..987
                     /locus_tag="VFV25_13195"
     CDS             559..987
                     /locus_tag="VFV25_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1394322.1"
                     /translation="MNSLTRRSALAFSLTTAGALPALLIPGPAAARRYRADAGEQIAP
                     GVRRVELSKRASEIPAYASVAMVDIVFQPKSKYANPSMASDMVCHCLEGELAIDQGTG
                     KAFLVKAGDVWSCGKGMPETTENRGTTVAIMRVIPLIAPA"
     gene            complement(1076..1498)
                     /locus_tag="VFV25_13200"
     CDS             complement(1076..1498)
                     /locus_tag="VFV25_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEY1394323.1"
                     /translation="MTRTSCLPLRAAFALGAAACLLAAALPAQANGHDEAMLKLASNS
                     GCLACHQVEPGAKGPDGLPPVGPPWREVAAKYKGRHGAADQLVQTVMKGSNPYDSHWK
                     GKASGLAMPPNAVAIKEADAKKLVAWILALDASKKSAP"
     gene            complement(1671..2723)
                     /gene="queA"
                     /locus_tag="VFV25_13205"
     CDS             complement(1671..2723)
                     /gene="queA"
                     /locus_tag="VFV25_13205"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEY1394324.1"
                     /translation="MPSVSRIFTLSDFDFALPPELIAQHPAPERSASRLLDGRGETPV
                     DRVFRELAQLLAPGDLLVFNDTRVIKARLYGAKASGGHVEALVERVLPDHEVLAHLRA
                     SKSPKPGSVVRFADAFDAEVLGRGGPDGGLFHLRFPDEPLALLARHGHVPLPPYITHA
                     DSADDERRYQTVFADKPGAVAAPTAALHFDAAVLTALREHGVNTASVTLHVGAGTFQP
                     VRVENLAEHKMHSEWFEVPEATVQAIAATRAAGGRVVAVGTTTLRALESAARDGELKS
                     GARDTDIFITPGFEFRVVDMLVTNFHLPKSTLMMLVAAFAGYEHVVALYRHAISGRYR
                     FFSYGDAMLLTRAEGR"
     gene            2776..>3032
                     /locus_tag="VFV25_13210"
     CDS             2776..>3032
                     /locus_tag="VFV25_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEY1394325.1"
                     /translation="MPEATSIPAAPQSKSQEKSAPQRVMEKLGLTRDTDLALHLPLRY
                     EDETRITRIADIRDGEAVQVEGVVTNSRVEFRPRRQLVVRL"
BASE COUNT          527 a         1005 c         1010 g          490 t
ORIGIN      
        1 gtggtcccag tgcccgtggg acaggacgat gcactcgacg cctcccatgt cgaagccgag
       61 gcgctcgacg ttgcgctcga acacgctgtc gtccggtccc gtgtcgaaca ggagggtgtg
      121 ggcggtgcca tcgcgccagg ccgtcacggc acaggatagg ccatgcgccg cacagcacat
      181 gcaccggccg gaccacagcc gaagcccccg gctccacagc cgcggcagtt cgttctccac
      241 gtagctgggc accagcgaga ggttgtcggt cgcgttgtca accagcacca ggacctcgac
      301 ccggtcggct gtggcaggct cgcgcatgcg gaactcctcg gctgcctcgc caacaagctt
      361 aggtcaccat tcggcaacct cgccccctca aaagagggaa tccgacccat cctgggtgta
      421 aacggacccc ttatcgccta tttttagatt tccagggagc agaggtgcgg ttcgcgcacc
      481 ctgtcgagca agcctcgacg ggattgccaa agtagggtgg tccgcgcggt acaggcgcac
      541 tcagcggagg agaaattcat gaacagtctg actcgtcgat ccgcacttgc cttctcgctg
      601 acgaccgcag gtgcgttgcc cgcactcttg atacccggcc cagccgccgc acggcgttac
      661 cgtgcagatg ccggcgaaca aatcgcgccc ggtgtcaggc gcgtcgagct atcgaagcgg
      721 gcttccgaga ttcccgctta cgcgagtgtg gcaatggtcg acatcgtttt tcagcccaaa
      781 tcgaagtatg cgaacccctc catggccagc gatatggtct gccattgcct ggaaggtgaa
      841 ttggcgatcg atcaaggtac tggcaaggcg ttcctggtca aggcaggaga cgtttggtcc
      901 tgcgggaaag gcatgccaga gacgacagag aaccgaggca ctaccgtcgc gatcatgcgc
      961 gtcatcccat tgatcgcgcc ggcctgatct ggacgcgccg aagcagtcgg tggtgagcga
     1021 gggctgaacg cacgcggccc gcttcccagc gagccgctcg ctcggcaagc agccgctaag
     1081 gcgccgactt cttgctcgcg tccaaggcca agatccaggc gaccagcttc ttcgcgtcgg
     1141 cctccttgat cgccacagca ttgggcggca tcgccagccc gctggccttg cctttccagt
     1201 ggctgtcgta gggattggag cctttcatca cggtctgcac cagctgatcg gccgcgccgt
     1261 gccggccttt gtacttggcg gccacttcgc gccagggcgg ccccacgggc ggcaggccgt
     1321 cggggccctt ggcgccgggc tcgacctgat ggcaggccag acagccgctg ttcgaagcca
     1381 gcttgagcat cgcctcgtcg tgaccgttcg cctgcgccgg cagcgcggcg gcgagcaagc
     1441 aggcggccgc gcccaaggca aacgccgccc gcaggggtaa gcagctcgtt ctcgtcatcg
     1501 tcgttctcct ggtgcgttgg aacgagtcca tgctaggcag ggctcatggc gccactttga
     1561 tgggcatcaa tgccggcctg tgttttgcca ggatggagca acgagttgcg gcggcgcgcc
     1621 gcgcctgcgc caagctggag cagctcggcg catgatgccg acgccgcggg tcagcgccct
     1681 tcggcgcgcg tcagcagcat cgcgtcgcca tagctgaaga agcggtagcg cccgcttatc
     1741 gcgtggcgat acagcgccac cacatgctcg tagccggcga aggcggccac cagcatcatc
     1801 agcgtgctct tgggcaagtg gaaattggtg acgagcatat ccacgacgcg gaactcgaag
     1861 cccggcgtga tgaagatgtc ggtgtcgcgc gcgccgcttt tcagctcgcc gtcacgcgcg
     1921 gccgattcga gcgcgcgcag cgtcgtcgtg ccgaccgcga caacgcgccc gccagcggca
     1981 cgcgtcgccg ctatcgcctg cacggtcgcc tcaggcactt cgaaccattc actgtgcatc
     2041 ttgtgctcgg cgaggttctc cacgcgcacc ggctggaagg tgccggcgcc cacgtgcagc
     2101 gtgacgctgg cggtgttcac gccatgctcg cgcaaggcag tcagcacggc tgcgtcgaag
     2161 tgcagggccg ccgtcggcgc agccaccgca cccggcttgt cggcaaacac ggtctggtag
     2221 cggcgctcgt cgtcggccga atcggcatgc gtgatgtagg gcggcagcgg cacgtggccg
     2281 tggcgcgcca gcagcgccag cggttcgtcc ggaaagcgca ggtgaaagag tccgccatcg
     2341 ggcccgccgc gtcccaacac ctcggcatcg aaggcatcgg cgaagcgcac cacgctgccc
     2401 ggcttgggcg acttgctggc gcgcagatgg gccagcactt cgtggtcggg caacacgcgc
     2461 tccaccaacg cctcgacatg cccgccgctg gccttggcgc catagaggcg agccttgatg
     2521 actcgcgtgt cgttgaagac cagcagatcg ccgggcgcga gcagttgcgc aagctcgcga
     2581 aacacgcggt ccacaggtgt ctcgccgcgg ccgtcgagca ggcgcgaggc gctgcgttcg
     2641 ggcgcggggt gctgggcgat gagttcggga ggcagagcga agtcgaagtc gctcagggtg
     2701 aagatgcggg aaacggaagg catggcgcgc aggctgtacc ggcctgtggc agtggagcaa
     2761 gtgcagaatt gtcccatgcc cgaggccacc agcatccccg ccgcgccgca gagcaaaagc
     2821 caagaaaagt ctgcgccgca gcgggtgatg gaaaagctcg gcctcacgcg cgacaccgat
     2881 cttgcgctgc acctgccgct gcgctacgag gacgagacgc gcattacgcg catcgccgac
     2941 atccgcgatg gcgaggcggt gcaggtcgaa ggcgtcgtca ccaacagccg cgtcgagttt
     3001 cgcccgcgcc gccagctcgt cgtgcggctg ga
//
LOCUS       DASUCY010000668         3973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1027183, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000668 DASUCY010000000
VERSION     DASUCY010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027183"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="VFV25_13215"
     CDS             <1..711
                     /locus_tag="VFV25_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEY1394326.1"
                     /translation="VLGGGLSILYTLAVHFGIEALHPARGALRQGVIFDLDERLDAAR
                     SARHQDMRDASVQQLQHRFAVDTSQAERVQALALALYAQAWPEADLETVRELGWAAAL
                     HELGMMVSHHDHHRHSAYLLGHVDAPGFSQSQQRRLGELVLAQRGGLRKMEVALAQTL
                     FAWQALCLRLAIILCHARSDVDPSAVALRRAGGSATLRLEPPWAEAHPRTLHLLNEEV
                     DTWARSGVLRLALRRAAG"
     gene            complement(720..1181)
                     /gene="sixA"
                     /locus_tag="VFV25_13220"
     CDS             complement(720..1181)
                     /gene="sixA"
                     /locus_tag="VFV25_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HEY1394327.1"
                     /translation="MDLILWRHAEAHLLREGQTDLQRALTTKGERQAQRMADWLNHRL
                     AESTRILVSPALRTQQTAQALERSYKTVHSIAPEASVDELLKAARWPDAAEPVLLVGH
                     QPTLGLLAARLLTGHELPWAIKKGAVWWLRQRTRDEETQVVLQAVQSPDCL"
     gene            complement(1201..3276)
                     /gene="ppk1"
                     /locus_tag="VFV25_13225"
     CDS             complement(1201..3276)
                     /gene="ppk1"
                     /locus_tag="VFV25_13225"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEY1394328.1"
                     /translation="MDAAVSVLPVAAVPPALDLLNREQAILEFNRRVLAQASRGDVPL
                     LERLRYVCIVSSNLDEFFEVRFADYLEAARLPTSTITKGELADVASAAHGLINEQYAL
                     FNDEIIPALKREGIVILNHAERNAEQRGWVENFFQKQVRPLLVPMGLDPAHPFPQVAN
                     KSLNFIAKLGGRDAFGRDNTIAIVKVPRVLPRVIRLPERLAPGQQAFVLLSSVIRAHL
                     ADLFPGRKVEAFSQFRVTRDSDLEVDEDDVTNLRQALRSSLSTRHFGQAIRLEVVNTC
                     PEELSEFLLQQFDLPDAALYRVNGPVNLVRLNQLIDQAEAPALRFPSYDPAWSSELPR
                     EASMFGRLRQGDVLLHHPFESFEPVVQLLREAVYDPDVLDIRQTIYRTGSESVLMDLL
                     LEAARRGKEVMAVVELKARFDEEANINWAERLEAVGAQVVYGIVGLKTHGKLLLITRR
                     EEGGRLRRYAHLSTGNYNPRTARLYTDVGYLTADPELTTDADTVFQQLASLTRQRAPK
                     QLLMAPFNLHRRMLQFIERVGAAAQAGKPARIVAKLNALTDVALIEALVAASQAGAEI
                     DLIVRGACMLPPGVPGVSERIRVRSIIGRFLEHSRVLYFRWGSGEADEVLYLSSADWM
                     SRNMFRRIEIAWPVRDAALRQRLIDECLVPYLHDRKDAWVLQPDGSHLRAGINGPSAQ
                     QALMKRYGG"
     gene            complement(3338..>3973)
                     /locus_tag="VFV25_13230"
     CDS             complement(3338..>3973)
                     /locus_tag="VFV25_13230"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEY1394329.1"
                     /translation="AHNDVVQKLLRKARKGTRVMFIPGNHDEFARKYLNHNFGGIEVV
                     EDWTHTTADGRRLWITHGDLFDGVIQCAKWLAYVGDSAYEFTLKLNRHFNSLRARLGL
                     PYWSLSKYLKLKVKRAVNFIGDFELAVAREARKRGVHGVVCGHIHHAELREIDGVLYA
                     NDGDWVESLTALVEHADGRLEILDWSGRALQARRAKAAAEEPVPAAAMAQV"
BASE COUNT          607 a         1344 c         1388 g          634 t
ORIGIN      
        1 gtgctgggcg gggggctgtc catcctctac acgctggcgg tgcatttcgg catcgaggcc
       61 ctgcatccgg cgcgcggggc actgcgtcag ggcgtgatct tcgacctcga cgagcgcctg
      121 gacgcggccc gcagcgcccg ccaccaggac atgcgcgacg cctcggtaca gcagctgcag
      181 caccgcttcg cggtcgacac cagccaggcc gaaagagtgc aggcgctggc gctggcgttg
      241 tacgcccaag cctggcccga ggccgatctg gagacggtgc gcgagctcgg ctgggccgca
      301 gcgctgcacg agctgggcat gatggtctcg caccacgacc accaccggca cagcgcctat
      361 ctgcttggcc atgtggacgc ccccggcttc tcgcagagcc agcaacgccg ccttggcgaa
      421 ctggtgctgg cccagcgcgg cggcctgcgc aagatggagg tggcgctggc gcagacgctc
      481 ttcgcctggc aggcgctgtg cctgcgcctg gccatcatcc tgtgccatgc gcgcagcgac
      541 gtcgatccca gcgccgtcgc gctgcgccgc gccggcggca gcgcaacgct gaggctggag
      601 ccgccctggg ccgaagccca cccgcgcaca ctgcatctgc tcaacgagga agtcgatacc
      661 tgggcgcgca gcggcgtgct gaggctggcg ctgcgccgcg cagcggggta gggcctgcgc
      721 tacaggcagt cggggctctg caccgcctgc agcacgacct gggtttcttc gtcgcgcgtg
      781 cgctggcgca gccaccacac ggcgcccttc ttgatcgccc agggcagctc gtggcccgtc
      841 agcaggcgcg cagcgagcaa gcccagcgtg ggctgatggc cgaccagcag cactggctcg
      901 gccgcgtcgg gccagcgcgc ggccttcagc agctcgtcca cgctcgcttc cggcgcgatc
      961 gaatggaccg tcttgtagct gcgctccagg gcctgggcag tctgctgggt gcgcagcgcc
     1021 gggctgacca ggatgcgcgt cgattcggcc agcctgtggt tgagccagtc ggccatgcgc
     1081 tgggcctggc gctcgccctt ggtggtgagt gcccgctgca gatcggtctg tccttcacgc
     1141 agcaggtggg cttcggcgtg gcgccagagg atgagatcca tggcgcccgc ctacccgacc
     1201 ctagccgccg tagcgtttca tcagcgcctg ctgggcgctg gggccgttga tgccggcacg
     1261 cagatggctg ccgtcgggct gcaataccca ggcgtccttg cggtcgtgca ggtagggcac
     1321 gaggcattcg tcgatcaggc gctggcgcag cgccgcgtcg cgcaccggcc aggcgatctc
     1381 gatgcggcgg aacatgttgc ggctcatcca gtcggcgctg gacaggtaca gcacctcgtc
     1441 ggcctcgccg gaaccccagc ggaaatagag cacgcggctg tgctcgagga agcgtccgat
     1501 gatggaacgc acgcggatgc gctcgctcac gcccggcacg ccgggcggca gcatgcaggc
     1561 gccgcgcacg atgaggtcga tctcggcacc tgcctggctg gccgccacca gggcctcgat
     1621 cagcgcgacg tcggtcagcg cgttgagctt ggcgacgatg cgcgccggct tgcctgcctg
     1681 tgccgctgcg ccgacccgtt cgatgaactg cagcatgcgc cggtgcaggt tgaagggcgc
     1741 catcagcagc tgcttgggtg cgcgctgccg cgtcaggctg gcgagctgct ggaacacggt
     1801 gtcggcatcg gtggtgagct cggggtcggc ggtcagatag ccgacgtcgg tgtagagccg
     1861 cgccgtcctg gggttgtagt tgccggtgga caaatgggca tagcggcgca gccgcccgcc
     1921 ctcctcgcgg cgcgtgatga gcagcagctt gccgtgggtc ttcaggccca cgatgccgta
     1981 caccacctgg gcccccaccg cctccaggcg ctcggcccag ttgatgttgg cctcctcgtc
     2041 gaagcgggcc ttgagctcga ccacggccat cacttccttg ccgcgccgcg ccgcctccag
     2101 cagcagatcc atcagcaccg actcgctgcc ggtgcggtag atggtctgcc ggatgtcgag
     2161 cacgtccggg tcgtagaccg cctcgcgcag cagctgcacc acgggctcga agctctcgaa
     2221 gggatggtgc agcagcacgt cgccctggcg cagccgcccg aacatcgagg cttcgcgcgg
     2281 cagctcgctc gaccacgccg ggtcatagga gggaaagcgc agcgccgggg cctcggcctg
     2341 gtcgatgagc tggttcagcc gcaccagatt caccggcccg ttgacgcgat agagcgccgc
     2401 atccggcaga tcgaactgct gcagcaggaa ttcgctcagc tcctcggggc aggtgttgac
     2461 cacttccagg cgtatggcct ggccgaagtg gcgggtactc agcgaggagc gcagggcctg
     2521 gcgcaggttg gtgacgtcgt cctcgtcgac ttcgagatcg gaatcgcgcg tgacgcggaa
     2581 ctgcgagaag gcctcgacct tgcgcccggg gaacaggtcc gcgagatggg cgcggatcac
     2641 gctggacagc agcacgaagg cctgttgccc cggcgccagc cgctcgggca ggcggatcac
     2701 gcgcggcagc acgcgcggca ccttgacgat ggcgatggtg ttgtcgcgcc cgaaggcatc
     2761 gcgcccgccg agcttggcga tgaagttcag cgacttgttg gcgacctgcg gaaagggatg
     2821 agccggatcc agccccatcg gcaccagcag cgggcgcacc tgcttctgga aaaagttctc
     2881 gacccagccg cgctgctcgg cgttgcgttc ggcgtggttg aggatgacga tgccctcgcg
     2941 cttgagcgcg gggatgatct cgtcgttgaa gagcgcgtat tgctcgttga tcagcccatg
     3001 cgcggcgctc gccacgtccg ccagctcgcc cttggtgatg gtggaggtgg gcaggcgcgc
     3061 ggcctccaga tagtcggcaa agcgcacctc gaagaactcg tccaggttgg acgagacgat
     3121 gcagacatag cgcagccgct ccagcagcgg cacatccccg cggctggcct gcgccagcac
     3181 gcggcggttg aattccagga tcgcctgctc gcggttgagc aggtccagtg ccggggggac
     3241 ggcggcgacg ggtagaaccg agacggctgc atccattgga cgcagtttat gacgccgcag
     3301 gggcgatttc gtgacagcgg gcggcgcctc gcagaactca cacctgcgcc attgcggcag
     3361 caggcaccgg ctcctcggcc gcggccttgg cgcggcgcgc ctgcagggcc cggcccgacc
     3421 agtcgaggat ctccagccgg ccgtcggcat gctcgaccag ggccgtcagg ctctcgaccc
     3481 agtcgccgtc gttggcatag agcacgccgt cgatctcgcg cagctcggca tggtggatat
     3541 ggccgcagac cacaccgtgc acgccgcgct tgcgcgcctc gcgcgccacg gcgagctcga
     3601 agtcgccaat gaagttgacg gcccgcttga ccttgagctt caagtacttc gacagcgacc
     3661 aatacggcaa gcccagccgc gcgcgcagcg aattgaaatg ccggttcagc ttaagcgtga
     3721 actcataggc ggagtcgccc acataggcca gccatttggc gcactggatg acgccgtcga
     3781 acaggtcgcc atgggtgatc cacagacgtc ggccatccgc ggtcgtatgc gtccaatcct
     3841 ccaccacctc gatgccgccg aagttgtggt tcaggtattt gcgcgcgaac tcgtcgtggt
     3901 tgccggggat gaacatcacc cgggtgccct tgcgcgcctt gcgcagcagt ttctgcacca
     3961 cgtcgttgtg ggc
//
LOCUS       DASUCY010000669         3969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_818185, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000669 DASUCY010000000
VERSION     DASUCY010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818185"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            259..738
                     /locus_tag="VFV25_13235"
     CDS             259..738
                     /locus_tag="VFV25_13235"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HEY1394330.1"
                     /translation="MTDKLSTWLRSLHPPVFVLALLFASYAVVLPLAFLQTAAPSLEL
                     GPGPANMQTMSLPWRLLLGSLVSPLLETALFQWAPIRLLRSRKLTLPWPAVLFVSAAL
                     FAAGHTYSTGYVVFAFLIGLVFAYGYAALDDEPRGMRAFVLVCLVHALRNGIASLLQ"
     gene            complement(749..1486)
                     /locus_tag="VFV25_13240"
     CDS             complement(749..1486)
                     /locus_tag="VFV25_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY1394331.1"
                     /translation="MPAIDASLAHHAPLRQRARAPTGAELSMVPWLQVLSPFARERVV
                     GELQVADAEVGELVCKIGRPATFWFGVIDGLLKMSNDSSVGAAITFTGVPPGGWFGEG
                     TLLKREAYRYNIQALRKSVVAGLPVDTFHWLLENSIPFNRFVLNQLNERLGQFIAARE
                     IDRLNDPDSRVARSLAALFHPTLYPGVGQLLRITQQELGYLVGLSRQRVNEALHALQE
                     AKLIRIEYGGVRVLDLEGLRRRVRQPA"
     gene            1672..3618
                     /locus_tag="VFV25_13245"
     CDS             1672..3618
                     /locus_tag="VFV25_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEY1394332.1"
                     /translation="METTFPRLMLEHAQRRPDAPALREKEYGIWQTLSWRELAELVRR
                     LALGLAAAGLKRGQHLVVVGENRPRLYASMLAAQSLGAIPVPLYQDAVAAEFVYPLNN
                     AEVAYAVVEDQEQVDKLIEIRAQCPQLARIWYDDPRGLRNYSEPGLASLDELSAEGEA
                     YATPHPELFETEIAKTQPEDVAAMFFTSGTTGNPKGVVHTHYSLLNRARAGARFDKLT
                     EKEEVLAYLPPAWIGQNCFSYAQWLVCGYVVNCPESASTVSIDLREIGPTYYFAPPRI
                     FEGLLTSVMIRMEDAAAPKRWLFQRFMDLARRVGPAKLDGKPIAFSDRLLYALGNVLI
                     YGPLRNTLGLSRIRVAYTAGEAIGPDLFSFFRAIGVNLKQLYGSTETAVFVCLQPDRE
                     AKADTVGVPIEGVEIKVDASGEVLIRSPGLLKEYYKNPQATAEVKTAEGWYRSGDAGF
                     LDASGHLKIIDRAKDVGRIVGGPNDGAMFAPKYVENKLKFFPYIKEAVALGDQRERVC
                     ALVNIDFEAVGNWAERRNLPYAGYTGLAAKPEVYELIRDCMQKVNADLAADEKLAGSQ
                     ISRFLVLHKELDADDGELTRTRKVRRGFIAEKYAVLVDALYGGKASQYIETQVKFEDG
                     RSGVVSAELRIVDVPTYPAVRRAA"
     gene            3618..>3969
                     /locus_tag="VFV25_13250"
     CDS             3618..>3969
                     /locus_tag="VFV25_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY1394333.1"
                     /translation="MTTAALRAAPMPFQEAVDAANDAANNAATTGRRIGDVVLEVQNI
                     SLAFGGVKALTDISFNVREHEIRAIIGPNGAGKSSMLNCINGVYQPQQGSITFRGRHV
                     KHMNSRQVAEMGVAR"
BASE COUNT          669 a         1363 c         1292 g          645 t
ORIGIN      
        1 ccccgacact atcttcatga gcttcgcagt attcatgggc ttccccctcc aaagtgtggt
       61 gggtttagag caaacgccgg gccaacggcg gcccatgttt cgtaagccat tgatgtgtaa
      121 cggtttttcc gcaaggccga cgtcatcgag gctcccgcag gcatggtgca aagcagcagg
      181 gtgtaaagaa tcccgacggt ttttttcacg atgccgaatc cgagcgcgct gtacggccga
      241 tgcaggagtc gacggccggt gaccgacaag ctctccacct ggctgcgtag cctgcacccg
      301 cccgtcttcg tgctggccct gctcttcgcg tcctacgccg tcgtcctgcc cctggccttc
      361 ctgcagaccg ccgcacccag cctggagctg ggcccgggcc ctgccaacat gcagaccatg
      421 agcctgccct ggcgcctgct gctgggctcg ctcgtttcgc cgctgctgga gacggcgctc
      481 ttccaatggg cgccgatacg cctgctgcgc agccgcaagc tcaccctgcc ctggcccgcc
      541 gtgctgttcg tttcggccgc gctgttcgcg gcgggccaca cctacagcac cggctacgtg
      601 gtcttcgcct tcctgatcgg cctggtcttc gcctacggct acgcggcgct ggacgatgag
      661 ccgcgcggca tgcgggcctt tgtgctcgtc tgcctggtgc atgcgctacg caacggcatc
      721 gcgtcgctgc tgcaatagga cacccgcttc aggcgggctg cctcacgcgc cggcgcaagc
      781 cttcgagatc gagcacccgc accccaccgt attcgatgcg gatcagcttg gcttcctgca
      841 gcgcatgcaa ggcctcgttg acgcgctgcc tggacaggcc caccagatag cccagctcct
      901 gctgggtgat gcgcagcagc tggccgacgc cgggataaag cgtcgggtgg aacagcgccg
      961 cgaggctgcg cgccacgcgc gaatccgggt cgttgaggcg gtcgatctcg cgtgcggcga
     1021 tgaactggcc caggcgctcg ttgagctggt tgagcacgaa gcgattgaag gggatgctgt
     1081 tctcgagcag ccaatggaag gtgtccaccg gcaggccggc cacgacgctc ttgcgcagcg
     1141 cctggatgtt gtagcggtag gcctcgcgct tgagcagcgt gccttcgccg aaccagccgc
     1201 ctggcggcac gccggtgaag gtgatggccg cgcccaccga gctgtcgttg ctcatcttca
     1261 ggaggccgtc gatcacgccg aaccagaagg tcgcggggcg gccgatcttg cagacgagct
     1321 cgccgacctc ggcatcggcc acctgcagct cgccgaccac gcgctcgcgc gcgaaggggc
     1381 tcagcacctg caaccagggc accatgctca gctcggcccc ggtgggtgcg cgggcgcgct
     1441 ggcgcagcgg ggcgtggtgc gcgagcgaag cgtcgatggc gggcatggct cgaatgggat
     1501 cgaagatcca ctgcgagggt aagtccccct cggacagacc cgaggattgt cgtcacaacg
     1561 acaacacggc gtcaaacttc tgcctagcat tcaacgcaaa gcaagccgcg cggccatagc
     1621 gagcctccct tccttctcat catcagcgcg gcatcaccgg taggagacgt ggtggagacg
     1681 acgttcccgc gcttgatgct cgaacatgcg cagcggcgcc ccgacgcgcc ggcgctgcgc
     1741 gagaaggaat acggcatctg gcagacgctg agctggcgag agctggccga gctggtgcgc
     1801 cgcctcgcgc tgggcctggc cgcggccggc ctgaagcgcg gccagcacct cgtcgtggtg
     1861 ggcgagaacc gcccgcggct ttatgcctcc atgctcgcgg cccagtcgct gggcgcgatt
     1921 cccgtgccgc tctaccagga cgcggtggcg gccgagttcg tctatccgct gaacaacgcc
     1981 gaggtcgcct atgccgtcgt cgaggaccag gagcaggtcg acaagctgat cgagatccgc
     2041 gcgcaatgcc cccagcttgc gcgcatctgg tatgacgacc cgcgcggcct gcgcaactac
     2101 agcgagcctg gcctcgcttc gctcgacgag ctgagcgcgg aaggcgaggc ctacgccacg
     2161 ccgcatcccg agctcttcga gaccgagatc gccaagaccc agcccgagga cgtggcggca
     2221 atgttcttca cctcgggcac caccggcaac cccaagggcg tggtgcacac gcattacagc
     2281 ctgctgaacc gcgcgcgcgc cggcgctcgc ttcgacaagc tcaccgagaa ggaagaagtg
     2341 ctggcctatc tgccgccggc ctggatcggg cagaactgct tctcctatgc gcagtggctg
     2401 gtgtgcggct atgtcgtcaa ttgtcccgag tcggccagca cggtgagcat cgacctgcgc
     2461 gagatcggcc cgacctatta cttcgcaccg ccgcgcatct tcgagggcct cctgaccagc
     2521 gtgatgatcc gcatggaaga cgcggccgcg cccaagcgct ggctgttcca gcgcttcatg
     2581 gacctggccc gccgcgtggg cccggccaag ctggacggca agcccatcgc tttcagcgac
     2641 cggctgctct atgcgctggg caatgtgctg atctacggcc ccttacgcaa caccctgggc
     2701 ctgtcgcgca tccgcgtggc ctacacggcc ggcgaggcga tcgggccgga tctgttcagc
     2761 ttcttccgcg ccatcggcgt caacctcaag cagctctacg gctccacgga aaccgccgtc
     2821 ttcgtctgcc tgcagccgga ccgcgaggcc aaggccgaca cggtgggcgt gccgatcgag
     2881 ggcgtggaga tcaaggtgga cgccagcggc gaggtgctga tccgttcacc cggcctgctc
     2941 aaggagtact acaagaaccc gcaggccacc gccgaggtca agacggccga gggctggtac
     3001 cgcagcggcg acgccggctt cctcgatgcg agcggccacc tgaagatcat cgaccgcgcc
     3061 aaggacgtgg gccgcatcgt cggcggcccc aacgacggcg cgatgttcgc gcccaagtat
     3121 gtggagaaca agctcaagtt cttcccctac atcaaggagg cggtggccct gggcgaccag
     3181 cgcgagcggg tctgcgccct cgtcaacatc gacttcgagg cagtgggcaa ctgggccgag
     3241 cggcgcaacc tgccctatgc cggctacacc ggcctggcgg ccaagcccga ggtctacgag
     3301 ctgatccgcg actgcatgca gaaggtcaat gccgacctgg ccgccgacga gaagctggcc
     3361 ggcagccaga tcagccgctt cctggtgctg cacaaggaac tcgacgccga cgacggcgag
     3421 ctgacgcgca cgcgcaaggt caggcgcggc ttcatcgccg agaagtacgc cgtgctggtc
     3481 gacgcgctgt atggcggcaa ggcctcgcaa tacatcgaga cccaggtgaa gttcgaggac
     3541 ggccgcagcg gcgtcgtctc ggccgagctg cgcatcgtcg acgtgccgac ctatcccgcc
     3601 gtgcggaggg ccgcctgatg acgactgctg ccctgcgcgc cgcgcccatg cccttccaag
     3661 aggcggtcga tgcggccaat gatgctgcca acaacgcggc gaccaccggt cgccgcatcg
     3721 gtgacgtggt tctcgaggtg cagaacatct cgctcgcctt cggcggcgtg aaggcgctga
     3781 ccgacatcag cttcaatgtc cgcgagcatg agattcgcgc catcatcggc cccaatggcg
     3841 cgggcaagag ctcgatgctg aactgcatca acggcgtcta ccagccgcag caaggctcca
     3901 tcaccttccg cggccggcat gtcaagcaca tgaattcgcg ccaggtggcc gagatgggcg
     3961 tggcgcgca
//
LOCUS       DASUCY010000670         2049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1429284, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000670 DASUCY010000000
VERSION     DASUCY010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1429284"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="VFV25_13255"
     CDS             <1..468
                     /locus_tag="VFV25_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY1394334.1"
                     /translation="AALPRDLRARTRPIAAKDYWTSSRLKQWLTREVFNAVYVDRQRK
                     DDQDPLEPLLEALDKGDSLVIFPEGTRSNKGEPQAFKSGLYHLAAQFPGVQLIPAWID
                     NVQRVMPKGEVVPVPILCTVTFGAPLQLQPDEDKQAFLARAREAVIALRSARS"
     gene            465..1439
                     /locus_tag="VFV25_13260"
     CDS             465..1439
                     /locus_tag="VFV25_13260"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEY1394335.1"
                     /translation="MSALRELSAQQQIALLFVILFGILLIATLAAFTSGLRERGEEGE
                     ERRARFRRDLRAVWGGAILFWLAWVSGPLGATLLFGVISFLALREYITLVNTRRSDHR
                     GLILAFFVVLPLQYLQVGLRSFDLFSVFIPVYVFLAIPVVSALAGDPERFLERNAKIQ
                     WGIMVCVYGLSHAPALLLLDFPRYGGRGAFLVFFLVFTVAAAQIAQEAASRWFRRRPL
                     ARQISRSFSLRAWLIGCGLAAFIGAALYWITPFRAGQAFMMALIASVAGTLGEFVMKA
                     LKRDAGVRYWGNRSSLTGAVGLLDRVAPLCFAAPVFFHSVRWYFRLVP"
     gene            1451..1999
                     /gene="ruvC"
                     /locus_tag="VFV25_13265"
     CDS             1451..1999
                     /gene="ruvC"
                     /locus_tag="VFV25_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003081116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEY1394336.1"
                     /translation="MRILGIDPGLRTTGFGIVEAEGARLHYVASGTIKTDSLPSGDLP
                     GRLKVLFAGVREVVQRYQPTCAAVEIVFVNANPQSTLLLGQARGAALAGLVSCELAVS
                     EYTALQMKKAVAGHGLARKEQIQEMVARLLKLPGAPGKDAADALGIAICHAHAGRSMA
                     ALAHATPLARKAHAQYRKGRTY"
BASE COUNT          293 a          687 c          695 g          374 t
ORIGIN      
        1 gccgcgctgc cgcgtgattt gcgagctcgg acccggccca tcgccgcaaa ggactactgg
       61 acctcgagtc gcctcaagca atggctcacg cgcgaagtct tcaacgcggt ctatgtggat
      121 cggcagcgca aggacgacca ggatccgctg gagccgctgc tagaagccct cgacaagggc
      181 gattcgctgg tcatctttcc cgaaggcacg cgcagcaaca agggcgagcc gcaggccttc
      241 aagagcgggc tgtatcacct ggccgcgcag tttccgggcg tgcaattgat cccggcctgg
      301 atcgacaacg tgcagcgcgt gatgcccaag ggtgaggtgg tgccggtgcc catcctgtgc
      361 actgtgacct tcggcgcgcc gctgcaattg cagccggacg aggacaagca ggccttcctg
      421 gcgcgcgccc gtgaggcggt gatcgcgctg aggagcgcgc gctcatgagc gcattgcgcg
      481 aactgagcgc gcagcagcag atcgcgctgc tcttcgtcat cctgttcggc attctgctaa
      541 tcgccacgct cgcggccttc acctctggcc tgcgcgagcg cggggaagaa ggggaggagc
      601 gccgggcgcg tttcaggcgc gacctgcgcg cggtctgggg cggcgccatc ctgttctggc
      661 tggcctgggt ctcggggccg ctcggcgcca ccttgctgtt cggcgtcatc tccttcctgg
      721 ccttgcgcga atacatcacg ctggtgaaca cgcgccgcag cgatcaccgc ggcctcatcc
      781 tcgccttctt cgtcgtactg ccgctgcaat acctgcaggt gggcctgcgc agcttcgatc
      841 tgttcagcgt cttcattccc gtctacgtgt tcctggcgat ccccgtggtc agcgcactgg
      901 cgggcgaccc ggagcgcttt ctggagcgca acgccaagat ccaatggggc atcatggtct
      961 gcgtctacgg cctgagccat gcgccggccc tgctgctgct cgacttcccg cgctatggcg
     1021 ggcgtggcgc cttcctggtc ttcttcctgg tgttcactgt cgccgcggcg cagattgcgc
     1081 aggaagcggc cagccgctgg ttcaggcgac gcccgctggc acggcagatc agccgctcct
     1141 tctcgctgcg cgcgtggctg atcggctgcg gcctggccgc tttcatcggc gccgcgctgt
     1201 actggatcac gcccttccgg gccggccagg ccttcatgat ggccttgatc gccagcgtcg
     1261 ccggcacgct gggcgagttc gtgatgaagg cactcaagcg cgatgccggc gtgcgctatt
     1321 ggggcaaccg ctcgtcgctc accggcgcgg tcggcctgct ggaccgcgtg gcgccgcttt
     1381 gcttcgcggc gccggtgttc ttccactcgg tgcgctggta tttcaggctc gtgccctgac
     1441 aatgaccgat atgcgcattc tcggcataga ccctggtttg cgaaccaccg ggttcggcat
     1501 cgtggaggcc gagggcgcgc ggctgcatta cgtggccagc ggcaccatca agaccgatag
     1561 cctgccgagc ggcgatctgc ccggccgcct caaggtcttg tttgctggcg tgcgcgaggt
     1621 ggtgcagcgc tatcagccga cctgcgctgc cgtcgagatc gtcttcgtca atgccaatcc
     1681 ccagtccacg ctgctgctgg gccaggcccg cggcgcggcg ctggcggggc tggtgagctg
     1741 cgagctggcg gtgagcgaat acaccgcgct gcagatgaag aaggccgtcg caggccatgg
     1801 cctggcgcgc aaggagcaga tccaggagat ggtggcgcgc ctgctcaagc tgccgggcgc
     1861 gcccggcaag gatgcggccg atgcgctggg tatcgcgatc tgccatgccc atgcgggccg
     1921 ctcgatggcc gcgctggcgc acgccacgcc gctggcgcgc aaggcccatg cgcagtaccg
     1981 caagggcagg acttactgag cactgaaagc gctccagccc ccgtggggtc gaggctcaga
     2041 atgcgccta
//
LOCUS       DASUCY010000671         3045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_962970, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000671 DASUCY010000000
VERSION     DASUCY010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962970"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VFV25_13270"
     CDS             complement(<1..233)
                     /locus_tag="VFV25_13270"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEY1394337.1"
                     /translation="MDNDEVLQLLGRIGQGDEAAFRELYRAFSRKLYAYVLRQLGDPA
                     RAEEIVADTLFEVWRQPARFRGDSQFSTWLIGIA"
     gene            complement(318..902)
                     /locus_tag="VFV25_13275"
     CDS             complement(318..902)
                     /locus_tag="VFV25_13275"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEY1394338.1"
                     /translation="MSMRLYIAQLTLALAAGSAMAQDVRLYGANEAVDPRDVVQILDQ
                     PSTGAPVIKMRSLRLLDDNAAPQTAGVAAASMVAAGAMPARALALPVQFAFDSAEILP
                     AARAQLDALAAGIRLLPANRAVIIEGHTDAAGSPRYNGLLSQRRAYSVKHYLVAMHGI
                     DPGRLQAVGLGMQMPLPGRDPYAPENRRVQFRGD"
     gene            complement(1096..1416)
                     /locus_tag="VFV25_13280"
     CDS             complement(1096..1416)
                     /locus_tag="VFV25_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1394339.1"
                     /translation="MSSPSERIAAGLAATTLIALVVLGAALLPRRSSAAEEPVRPANA
                     AVFQVRCWQYGRLLFEQRDVRLPNELPTGLKLHGFDRNRQPVYITDTGNATCLIRSVA
                     TPKP"
     gene            complement(1400..2959)
                     /locus_tag="VFV25_13285"
     CDS             complement(1400..2959)
                     /locus_tag="VFV25_13285"
                     /inference="COORDINATES: protein motif:HMM:NF025684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HEY1394340.1"
                     /translation="MKPPTPSTARRLVITAAMAVAFAGCSTLDVRQPTIEQTAAAKQG
                     PEAAPYRSITGFSSALRCMDNLMVDYGVHDVTMLVEEIGDQTKKVNAGTRDMLITAVS
                     DMTRRSRAVRVVAYGKDATNVISFLASAQRQSAYEVIPQYDIKGSVSQLDENVIRNQK
                     DLGIGYQPFINIGVSRDAASSILGLDLSVLTTDDMSILPGVTSRNSVVILKQGKGFDG
                     DAAYSKFGVNYTMNFSKSEGQSQALRGLVELAVVELAGKLTKTPYWSCLDADAQANEE
                     ISQEIADWYFAMAASRSELIGYFQNQLRQRGFYEGPIDGKFNPAIDEAIANYRAALGL
                     SREALLDEKFFRAYLESDHAKISRPAKPASLAEMLPATTDKAAAPTLRLALTAANRQT
                     RYAGGEPVNLTVQPTRDSHIYCYLQDENAQIRRFYPNRFNRDSLVPADQALAIPGSMR
                     FQLVMNTKGVSETVTCFATAQDVLQMLPNSVVGVDFEALPVATLDQIRNAFFTVSGGL
                     LAQETFHVQPK"
BASE COUNT          484 a          941 c         1061 g          559 t
ORIGIN      
        1 gcgatgccga tcagccaggt gctgaactgc gaatcaccgc gaaagcgagc gggctgacgc
       61 cacacttcaa acagggtatc ggccacgatt tcctccgccc gggctggatc gccgagttgc
      121 ctcagcacat aggcgtagag cttgcgcgag aaagcgcgat acagctcgcg gaacgccgct
      181 tcatcgccct gcccgatgcg gccgagcagc tgcaacacct cgtcgttgtc catgtttgga
      241 acgccaccag ggcgtgaggt gaatgacgtt cgcgcatggt aaatctcctc gcccttgcgg
      301 tggggatgtt tgcggcgtca gtcgccacgg aactgcacac ggcggttctc cggcgcatag
      361 gggtcgcgac cgggcagcgg catctgcatg cccaggccta ccgcctgcag ccggcccggg
      421 tcgatgccgt gcatcgccac gaggtagtgc ttgaccgaat aggcgcgccg ctgcgacagc
      481 agcccgttgt agcgtggcga ccctgcggcg tccgtgtggc cttcgatgat cacggcccgg
      541 ttggccggca gcagacggat gccggcagcg agcgcatcga gctgggcacg cgccgcgggc
      601 aggatctccg ccgaatcgaa ggcgaactgc actggcaggg ccagcgcgcg ggccggcatg
      661 gcgccggcgg ccaccatgga tgctgccgcg acacctgcgg tctgcggtgc agcgttgtcg
      721 tcgagcagcc tcagcgagcg catcttgatc accggagcgc cggtactcgg ctgatcgagg
      781 atctgcacca catcccgtgg atcgaccgct tcgttcgcgc cgtacaagcg cacgtcttgg
      841 gccatggccg acccggcggc gagagcgagg gtcagctggg cgatatacag gcgcatcgac
      901 atgatggtct ccgatctgtt ggtggggttc ggtcatgggt tgcagcgagg cggccgcggg
      961 ttcaggtttg tttgctgaat agcgcgcgcg ttcttgtgcg gtgtgcgcag ggcagcccag
     1021 gcgccggcgg tcactcgcgg cgctgggtgc actcggtgcg gccccggttc actggccgcc
     1081 actcctcccc aagcttcagg gcttgggcgt tgcgacgctg cggatgaggc aggtggcgtt
     1141 gccggtgtcg gtgatgtaga ccggttggcg gttgcgatcg aagccgtgca gcttcagccc
     1201 cgtcgggagc tcgttgggca gccgtacgtc gcgctgctcg aacagcagcc gcccgtactg
     1261 ccagcagcgc acctggaaca cagccgcatt ggcgggccgc acgggctcct cggcggcgct
     1321 gctgcggcgc ggcagcagcg cggcgcccaa gaccaccaga gcgatcaggg tggtggcggc
     1381 aaggccggca gcgatgcgct cacttgggct ggacatggaa ggtctcctgg gcgagcaagc
     1441 cgccgctcac agtgaagaag gcgttgcgga tctggtcgag cgtggcaacc ggcagcgctt
     1501 cgaagtcgac gccgaccacc gagttgggca gcatctgcag gacgtcttgt gcggtggcga
     1561 agcacgtcac cgtttcgctc actcccttgg tgttcatcac cagctggaag cgcatcgagc
     1621 cgggtatggc gagcgcctgg tcggccggca ccagcgaatc acggttgaag cggttcggat
     1681 agaagcgccg gatctgcgcg ttctcgtcct gcaggtagca atagatgtgg gagtcgcggg
     1741 tgggctgcac tgtcaggttg accggctcgc cgccggcata acgggtctgc cggttggccg
     1801 ccgtcaatgc aaggcgcagc gtcggcgcgg cggccttgtc ggtcgtggcc ggcagcatct
     1861 ccgccaggga cgcaggcttc gcggggcgcg aaatcttggc atggtccgac tcgaggtagg
     1921 ccctgaaaaa cttctcgtcc agcagcgcct cgcggctcag gcccagcgcg gcgcggtagt
     1981 tggcaatggc ctcgtcgatg gccgggttga acttgccgtc gatcggcccc tcgtaaaagc
     2041 cgcgctggcg caactggttc tggaaatatc cgatcagctc gctgcggctg gcggccatgg
     2101 cgaagtacca gtcggcgatc tcctggctga tctcctcgtt ggcctgggcg tcggcatcga
     2161 ggcagctcca atagggggtc ttcgtcagct tgcccgccag ctcgacgacc gccagctcga
     2221 ccaggccgcg cagggcctgc gactggccct cggacttgct gaagttcatg gtgtagttga
     2281 cgccgaactt gctgtaggcc gcatcgccgt caaagccctt gccctgcttg agtatcacca
     2341 ccgagttgcg cgaggtgacg ccgggcagga tcgacatgtc gtcggtcgtg agcacgctga
     2401 ggtccagacc gaggatggac gatgcggcat cgcgcgacac gccgatattg atgaaaggct
     2461 ggtaccctat gcccaagtcc ttctgattgc gtatcacgtt ctcgtcgagc tgcgaaaccg
     2521 agcccttgat gtcgtactgg gggatcactt cgtaggcgct ttgtcgctgg gccgacgcca
     2581 ggaaactgat gacattggtg gcgtccttgc cgtaggcgac cacgcgcacg gcacggctac
     2641 gtcgggtcat gtccgacact gcggtgatca gcatgtcgcg ggtgccggcg ttgaccttct
     2701 tggtctggtc gccgatttcc tcgaccagca tggtgacgtc gtgcacgccg tagtcgacca
     2761 tcaggttgtc catgcagcgc agcgcggatg aaaagccggt gatcgagcga tacggggccg
     2821 cttccgggcc ttgcttcgcg gccgccgtct gttcgatcgt cggctgtctg acgtccagcg
     2881 tggaacagcc ggcgaacgcc acggccatcg cagcagtgat cacgaggcgg cgcgctgtcg
     2941 atggcgtggg cggtttcatt tgggttcttt ctctttcgat agcgccggct tacttgcagc
     3001 cgttgcccga caccaggttg atggcgttgg gggcgaagac gaaga
//
LOCUS       DASUCY010000672         2925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1541228, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000672 DASUCY010000000
VERSION     DASUCY010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1541228"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="VFV25_13290"
     CDS             <1..672
                     /locus_tag="VFV25_13290"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEY1394341.1"
                     /translation="KTGLPVVIVISEIFGVHEHIADVARRFAKLGYLAIAPELFVRQG
                     DAKSYADMSRLVAEVVSKVPDAQVMGDLDAAVAWAKAHGGDIGRLGVTGFCWGGRIAW
                     LFAERRPAVKAAVAWYGRLLGPTNDNSPRHPIDLVASLKAPVLGLYGGQDQSISLESV
                     EKMKAALAQAGKAGNAAAAKSEFVVYPEAGHAFNADYRPSYNKAAAEDGWKRCLAWFK
                     ANGVA"
     gene            complement(704..970)
                     /locus_tag="VFV25_13295"
     CDS             complement(704..970)
                     /locus_tag="VFV25_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1394342.1"
                     /translation="MTLQQLQALKRWHVAHRRDHPLEYHVFDAVLTVWMLGWVGLPPA
                     LLLKGPALLPLCLAAFLAPELYRALRLWLHRSHRLRCDWLVALG"
     gene            complement(1074..2231)
                     /locus_tag="VFV25_13300"
     CDS             complement(1074..2231)
                     /locus_tag="VFV25_13300"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEY1394343.1"
                     /translation="MGVITCTEDLRVLAKKRVPRMFYDYADSGSWTEGTYRANEADFQ
                     RIKLRQRVAVNMEGRSIRTQMIGQDVAMPVAIAPTGLTGMQHADGEILAARAAEKFGI
                     PFTLSTMSICSIEDVAAHTKAPFWFQLYVMRDRGFIERLIDRAKAARCSALVLTLDLQ
                     ILGQRHKDLKNGLSAPPKPTLANLLNLMTKPRWCLGMLGTRRHSFGNIVGHAQGVGDL
                     SSLSAWTSQQFDPKLNWGDVEWIKQRWGGKLILKGILDVEDARLAADSGADALIVSNH
                     GGRQLDGAPSSIAALPAIAEAVGHRIEVWMDGGIRSGQDVLKAWALGARGTMIGRSFL
                     YGLGALGEAGVTKCLEIIANELDLTMAFCGRTRLADVDKSVLLPGSYPTAG"
     gene            complement(2341..2478)
                     /locus_tag="VFV25_13305"
     CDS             complement(2341..2478)
                     /locus_tag="VFV25_13305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1394344.1"
                     /translation="MTDKQRHHLHQQTPGRAPIAGLPNWLILGSAALVAVAAALLRAL
                     G"
     gene            complement(2670..>2925)
                     /locus_tag="VFV25_13310"
     CDS             complement(2670..>2925)
                     /locus_tag="VFV25_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEY1394345.1"
                     /translation="LKLRYDNFKTVTRDYTIGHYTADAQAIRRAAGVCLKRVPLERRL
                     RLLGVRVGALVKASEAPVSTAPAAVMAQEAEAPYTPDLFA"
BASE COUNT          458 a          943 c         1043 g          481 t
ORIGIN      
        1 aaaaccggcc tgccggtggt gatcgtgatc tccgagatct tcggcgtgca cgagcacatc
       61 gccgatgtgg cgcgccgctt cgccaagctg ggctatctgg cgatcgcccc cgagctgttc
      121 gtgcgccagg gcgatgccaa gtcctatgcc gacatgtcgc gcctggtggc cgaggtggtc
      181 agcaaggtgc ccgacgccca ggtgatgggc gacctggatg ccgcggtggc ctgggccaag
      241 gcccacggcg gcgatatcgg ccgcctgggc gtgaccggct tctgctgggg cggacgcatc
      301 gcctggctgt tcgccgagcg ccgtcccgcg gtgaaggccg ccgtggcctg gtatggcagg
      361 ctgctcgggc cgacgaacga caacagcccc aggcatccca tcgatctggt cgcttcgctc
      421 aaggcgccgg tgctgggcct gtacggcggg caggaccaga gcatctcgct ggagtcggtg
      481 gagaagatga aggccgcgct ggcacaggcc ggcaaggcgg gcaatgcggc cgccgccaag
      541 tcggaattcg tggtctatcc cgaggccggc catgccttca acgccgacta ccgccccagc
      601 tacaacaagg cggcggccga ggacggctgg aagcgctgcc tggcctggtt caaggcgaac
      661 ggcgttgcct agaagcggat catcggcggg cggggtgccg tcatcagcct agcgcaacca
      721 gccagtcgca gcgcagccgg tggctgcggt gcagccacag cctcagggcc cggtagagct
      781 cgggtgccag aaaggccgcc aggcacagcg gcaggagggc ggggcccttc agcagcaggg
      841 ccggcggcag gccgacccag cccagcatcc agacggtcag caccgcgtcg aagacgtggt
      901 attccaacgg gtggtccctg cggtgcgcaa catgccagcg cttgagggcc tggagctgct
      961 gcagcgtcat gatcgcgatc tcgacggagc tgtcggccca gtttaggagg ctcgccctcg
     1021 cgtggatacc ccgcgccggg tgttttacaa gcaatgcgtc cagccggcgg gcctcagccc
     1081 gcggtcggat agctgcccgg cagcaggacg ctcttgtcca cgtcggccag ccgcgtgcgt
     1141 ccgcagaagg ccatggtcag gtcgagttcg ttggcgatga tctccaggca tttggtgacg
     1201 ccggcctcgc ccagcgcgcc caggccatag aggaagctgc gcccgatcat ggtgccgcgc
     1261 gcgcccagcg cccaggcctt gagcacgtcc tggccgctac gtatgccgcc gtccatccag
     1321 acctcgatgc ggtggccgac cgcttcggcg atcgccggca gcgccgcgat cgatgagggc
     1381 gcgccatcga gctggcgccc gccgtggttg gagacgatca gggcatcggc gccggaatcg
     1441 gccgccaggc gtgcgtcctc gacgtcgagg atgcctttga ggatcagctt gccgccccag
     1501 cgctgcttga tccattccac gtcgccccag ttgagcttgg ggtcgaactg ctgggaggtc
     1561 catgcgctca gggaagaaag gtcgcccacg ccctgggcat ggccgacgat gttgccgaag
     1621 ctgtggcggc gcgtgcccag catgcccaga caccagcgcg gcttggtcat caggttgagg
     1681 aggttggcca gcgtgggctt gggcggcgcg ctcaagccgt tcttcaggtc cttgtggcgc
     1741 tggccgagga tctgcaggtc cagcgtcagc accagggcgc tgcacctggc cgccttggcg
     1801 cggtcgatca ggcgctcgat gaagccgcgg tcgcgcatca catagagctg gaaccagaac
     1861 ggcgccttgg tgtgggcggc cacgtcctcg atcgagcaga tgctcatcgt cgacagcgtg
     1921 aaggggatgc cgaacttctc ggccgcgcgc gccgccagga tctcgccgtc ggcatgctgc
     1981 atgccggtga gcccggtggg cgcgatggcc actggcatcg ccacgtcctg gccgatcatc
     2041 tgcgtgcgga tcgagcggcc ttccatgttg acggccacgc gctggcgcag cttgatgcgc
     2101 tggaagtcgg cctcgttggc gcggtaggtg ccctcggtcc aggagccgga gtcggcatag
     2161 tcgtagaaca tgcgcggcac gcgcttcttg gccagcacgc gcaggtcttc ggtgcaggtg
     2221 atcacgccca tgagtctccg tcttggtctt gtcatgaaga cgcagactct agcagtgcga
     2281 ggccgcaccg cggcggccga agctgcggcc gagggccgcc gcctcgagga cgggcgaagt
     2341 tcagcccagg gcgcgcagca gggcggcggc aacggcgacc agggcggccg agccgaggat
     2401 cagccagttg ggcaggccgg cgatcggtgc gcggcccggc gtttgctgat gcaggtggtg
     2461 acgctgtttg tcagtcatgg agaactccca acgacgtcct gggtcgcttc ccgcgttccg
     2521 cgggagccag gatcaggttt ggagtcctaa aagcgatctt tgtgccaggc tccaccgcgc
     2581 tggtttcgat cgttaggttg ctataaattc gatatcggat attggcttaa ataaacggcg
     2641 ttgctgcaga aaatctgcag aacctgcgct caggcgaaca agtccggggt gtacggcgct
     2701 tcggcctcct gggccatcac cgcggccggc gcggtcgaaa ccggtgcctc gctcgccttg
     2761 accagtgcgc cgacgcgcac gcctagcagg cgcagccgcc gctccagcgg cacgcgcttg
     2821 aggcagacgc cggccgcgcg gcgtatggcc tgcgcatcgg ccgtgtagtg gccgatcgtg
     2881 tagtcgcgcg tgacggtctt gaagttgtcg tagcgcagct tcagg
//
LOCUS       DASUCY010000673         3391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_497134, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000673 DASUCY010000000
VERSION     DASUCY010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497134"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VFV25_13315"
     CDS             <1..291
                     /locus_tag="VFV25_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEY1394346.1"
                     /translation="ENGSVRRDGLAVNFLVTDTVQTIPVRYQGILPDLFKEGKGVVAQ
                     GQIGADGVFVAHEVLAKHDENYMPPEAADALKRARQVNDKIAASVIKDPGKP"
     gene            288..2279
                     /locus_tag="VFV25_13320"
     CDS             288..2279
                     /locus_tag="VFV25_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEY1394347.1"
                     /translation="MIPELGQIALLLALAVALIQGSLPLLGASRGRADWMSLARPAAQ
                     THFVLVGIAFLCLASCFVRNDFSVLYVASNSNSALPLYYRIAGVWGGHEGSLLLWLMM
                     LSVWMLAVAQLSRHLPRPVVARILAVMGLISVGFLLFMLTTSNPFERLFPVPPDGNDL
                     NPLLQDPGMVAHPPMLYMGYVGFSVAFAFAVAALLGGNLDATWARWTRPWTTAAWTFL
                     TIGIMLGSMWAYYVLGWGGWWFWDPVENASFMPWLAGTALIHSLAVTEKRGAFKSWTV
                     LLAIVAFSLSLLGTFLVRSGVLSSVHAFATDPRRGLFILIFLAVVIGGSLALYAWRAP
                     KVGLGARFGLVSRETMLLGNNVLFVVAMLSVLLGTLYPLLLDSLGMGKISVGPPYFDT
                     VFVPLMAPVVFLLGVGPLARWKQAEIPDLAKRLRWAAAVAVVAALATGWLAGRISWGA
                     TLGFLMAYWILASTATDLWERVRPAGGVETKLVARLRLLPRAMVGMMIAHLGVAAFAF
                     GVSMVKTYEVERDVKMDVGDTTELAGYVFAFHGVKDVQGPNYDGVQGLVEVTRKGRPV
                     VQLKPEKRIYRVQRTPMTEAAIDPGPTRDLYVSLGEPIVGSGAWIVRVYVKPFVDWIW
                     GGCLLMAIGGALAASDRRYRVGARAAQPQTTGATSGAEA"
     gene            2276..2806
                     /locus_tag="VFV25_13325"
     CDS             2276..2806
                     /locus_tag="VFV25_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HEY1394348.1"
                     /translation="MKSLRFILPLAVFAILLGFLWRGLRLDPHEVPSPLINKPAPGFA
                     LTRLDDPSATLRRDDMLGKVWMLNVWASWCVACREEHPTLVAFSRTKAVPIIGLNYKD
                     TRPEGLGWLNQFGNPYEASVFDQDGRVGIDFGVYGVPETFIIDKQGVIRFKQIGPLTP
                     QAINERVLPLLRQLNG"
     gene            2799..3254
                     /locus_tag="VFV25_13330"
     CDS             2799..3254
                     /locus_tag="VFV25_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HEY1394349.1"
                     /translation="MAKLLACLLLGLLSLVACAKDAAPAVDDPLLEAHMLRITGELRC
                     LVCQNQTIADSHAGLAEDLREEVRVMLRKGESDEQIVKYMTDRYGDFVLYRPPLKATT
                     LLLWFGPAALLLIGATSLILVLYRRSRMSDDRFEPDELDDRSAEQGSTS"
BASE COUNT          467 a         1158 c         1134 g          632 t
ORIGIN      
        1 gagaacggca gcgtgcggcg cgacgggcta gcggtgaatt tcctggtgac cgataccgtg
       61 cagaccatac cggtgcgcta ccagggcatc ctcccggacc tgttcaagga gggcaagggc
      121 gtggtggcgc aggggcagat cggcgcggac ggcgtcttcg tcgcccatga agtgctggcc
      181 aagcacgacg agaactacat gccgcccgag gccgccgatg cgctgaagcg ggcgcgccag
      241 gtcaatgaca agatcgccgc ctcggtgatc aaggacccag gcaagccatg attcccgagc
      301 ttggtcagat cgcgctgctg ctggcgctcg ccgtggccct gatccagggc agcctgccgc
      361 tgctgggcgc gtcccgcgga cgggccgatt ggatgtcgct cgcgcgcccc gcagcgcaga
      421 cgcatttcgt gctggtgggc atcgccttcc tgtgcctggc gagctgcttc gtgcgcaacg
      481 acttctcggt gctctacgtg gcgtccaatt ccaacagcgc cctgccgctg tactaccgca
      541 tcgccggtgt ctggggcggc cacgagggct cgctgctgtt gtggctgatg atgctcagcg
      601 tctggatgct ggccgtcgcg cagctgagcc gccatctgcc acggcccgtc gtggcgcgca
      661 tcctcgcagt gatgggcttg atcagcgtgg gcttcctgct gttcatgctc accacgtcca
      721 atcccttcga gcgcctgttc ccggtgccgc cggatggcaa cgacctgaac ccgctgctgc
      781 aggacccggg catggtggcg catccgccca tgctctacat gggctatgtc ggcttctcgg
      841 tggccttcgc ctttgccgtg gcagcgctgc tgggcggcaa cctggacgcc acctgggcgc
      901 gctggacgcg accctggact acggcggcct ggaccttcct caccatcggc atcatgctcg
      961 gcagcatgtg ggcctattac gtgctgggct ggggcggctg gtggttctgg gatccggtgg
     1021 agaacgcttc cttcatgccc tggctggccg gcacggcgct gatccattcg ctggccgtga
     1081 ccgagaagcg cggcgctttc aagtcctgga ccgtgctgct ggccatcgtc gccttctcgc
     1141 tgtcgctgct cggcaccttc ctcgtgcgct cgggcgtgct ctcctcggtg catgccttcg
     1201 ccaccgatcc gcgccgcggc ctgttcatcc tgatcttcct cgccgtcgtg atcggcggct
     1261 cgctggccct gtatgcctgg cgcgcgccca aggtcgggct gggcgcccgc ttcggcctcg
     1321 tctcgcgcga gaccatgctg ctgggcaaca acgtgctgtt cgtcgtggcg atgctgtcgg
     1381 tgctgctggg cacgctctat ccgctgctgc tcgattcgct gggcatgggc aagatttcgg
     1441 tcggaccgcc ctatttcgac accgtcttcg tgccgctgat ggcgccggtg gtcttcctgc
     1501 tcggcgtagg ccctctggcg cgctggaagc aggccgagat tcccgacctc gccaagcgcc
     1561 tgcgctgggc ggccgccgtg gcggtggtcg ccgccctggc gaccggctgg ctggccggcc
     1621 gcatttcttg gggcgccacg ctcggcttcc tgatggccta ttggatcctg gcttccaccg
     1681 ccaccgacct gtgggagcgc gtgcggcctg ccggcggcgt cgagacgaag ctcgtggcgc
     1741 gcctgcgcct gctgccgcgc gccatggtgg gcatgatgat cgcccacctc ggcgtcgcgg
     1801 ccttcgcctt cggcgtgagc atggtgaaga cctatgaggt cgagcgcgac gtgaagatgg
     1861 atgttggcga caccactgaa ctggccggct acgtcttcgc attccacggc gtcaaggatg
     1921 tgcaggggcc caactacgac ggcgtgcagg gcctggtcga ggtgacgcgc aagggccgtc
     1981 ccgtggtgca actgaagccc gagaagcgca tctaccgcgt gcagcgcacg cccatgaccg
     2041 aggcggcgat cgaccccggc ccgacgcgcg atctctacgt ctcgctcggc gagcctatcg
     2101 tgggcagcgg cgcctggatc gtgcgcgtct atgtcaagcc cttcgtcgac tggatctggg
     2161 gcggctgcct gctgatggcg atcggcggcg ccctggccgc cagcgaccgc cgctatcgcg
     2221 tcggcgcaag ggccgcgcag ccgcagacga ccggcgcaac gtcgggggcc gaggcatgaa
     2281 gagcctgcgc ttcatccttc cgctcgctgt gttcgcgatc cttttgggct ttctgtggcg
     2341 cggcctgcgg ctcgacccgc acgaggtgcc ttcgccgctg atcaacaagc ccgcacccgg
     2401 cttcgcgctg acgcggctgg acgatccctc tgccaccctc cggcgcgacg acatgctggg
     2461 caaggtctgg atgctcaacg tctgggcctc atggtgcgtc gcctgccgcg aggagcatcc
     2521 cacgctggtg gccttctcgc gcaccaaggc cgtccccatc atcggcctga actacaagga
     2581 cacccgcccc gagggtctgg gctggctgaa ccagttcggc aatccctacg aggcctcggt
     2641 gttcgaccag gacgggcgcg tgggcatcga cttcggcgtc tacggcgtgc ctgagacctt
     2701 catcatcgac aagcagggcg tgatccgctt caagcagatc ggcccgctca cgccccaggc
     2761 catcaacgag cgtgtgcttc ccctgttgag gcagctcaat ggctaagctc cttgcttgcc
     2821 tgctgctggg cctgctgtcc ctggtcgctt gcgccaagga cgcggcaccg gccgtcgacg
     2881 atccgctcct cgaggcgcac atgctgcgca tcaccggcga gctgcgctgc ctggtctgcc
     2941 agaaccagac catcgccgat tcgcatgccg gccttgccga ggacttgcgc gaggaggtgc
     3001 gcgtgatgct gcgcaagggc gagagcgacg agcagatcgt caagtacatg accgaccgct
     3061 atggcgactt cgtgctctac cgcccgccgc tcaaggccac caccctgctg ctgtggttcg
     3121 gccccgctgc gctgctgctg atcggcgcca cgagcctgat tctggtgctg taccggcgct
     3181 ccaggatgag cgacgatcgc ttcgaacccg acgaactcga cgaccgcagt gcggagcagg
     3241 gaagcacgtc atgacggcct ttttcctaat gatcgccgcg gcgatgtgcc tgctggcggc
     3301 cgcgctgctg acgcggccgt tgtggtggcc tttcggcaag acgaaacggc atgcgcacag
     3361 cgaacaagag cgtgaggagc aatccggcgc c
//
LOCUS       DASUCY010000674         2337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_914586, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000674 DASUCY010000000
VERSION     DASUCY010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914586"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(355..564)
                     /locus_tag="VFV25_13335"
     CDS             complement(355..564)
                     /locus_tag="VFV25_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1394350.1"
                     /translation="MNKLLAALIASAFAVGAYAQAPAAPAAASAPAKAEAKAAEGKME
                     KKAPAKKHHHMKAKKAAAAEKAASS"
     gene            complement(701..1753)
                     /locus_tag="VFV25_13340"
     CDS             complement(701..1753)
                     /locus_tag="VFV25_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012435430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEY1394351.1"
                     /translation="MSSPAGPHYADDADCLAGLRIVDLTRLLPGPLATLRLAQWGAEV
                     IKIEDPGEGDGARALWRSAAQERSGEPGPFFRELNAGKQLRRIDLHSEAGRAELLELV
                     READALVEGFRPGVMDRLGLGWDTLSAVNPRLVMVSISGYGQQGPWAQRAGHDINYIA
                     MAGLLQQIATSKGEPALPNFQIGDLLGGTQAALSALLAALLGAQRSGRGRHIDISMSH
                     ELLRHQVVLRAGLAARGELPPPGRDLLSGGAPCYGLYRCGDGRYLALGALELKFWRNF
                     CTALARPDWVQQHWSLGQAPGGAAALALRDEVEALLITRPLAHWLNLLEAADACVTPV
                     LSLDEALAHPLFADES"
     gene            complement(1750..>2337)
                     /locus_tag="VFV25_13345"
     CDS             complement(1750..>2337)
                     /locus_tag="VFV25_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEY1394352.1"
                     /translation="IVMAMRNPRVVVFGELLSDEECDAIVALARQRLSRSETVDTATG
                     GSEINASRTSEGMFFRRAENELIARVERRIAALLNWPLENGEGLQILYYRPGAEYKPH
                     YDYFDPAQPGSAPILKRGGQRVGTLVIYLNDPVKGGGTTFPDVALEVAAVKGSAVFFS
                     YDKPHPTTRTLHGGAPVVEGEKWVATKWLRVGRFE"
BASE COUNT          379 a          793 c          793 g          372 t
ORIGIN      
        1 cctgccgcgc accgagctcg ggccgctgat cgagaaatgg gcgctggaag gcccccagac
       61 ctgggatcag cgccgctggc gccgcatcga aaacgaccgc cctttcgggc caccgagctg
      121 gtccacgcgc gatcgcagca caggcgaaca gcctcacccc tgaagcggag ctcagccgcc
      181 attcgacgac ttttccgcag gtaggccctt ctcttctgcg aaaaatcgct atctattctg
      241 tagcgttacg attggcgcct ggcgcggcat aaccagcgcc caaacgaaac aacccggcgc
      301 gaggccgggt tgttgtcgac ggggttcggg gccagcccga tccgtaaagc cctattagga
      361 cgaagcggcc ttctcggcag cagcggcctt cttggccttc atgtggtggt gcttcttggc
      421 aggagccttc ttctccatct tgccttcggc ggccttggct tcagccttgg cgggagcgga
      481 ggcggcagcg ggagcggccg gggcttgggc gtaagcacca acggcgaagg cggaagcgat
      541 cagggcagcg agaagtttgt tcacggttgt ggctcctaga ggaaagatgc atctttcgag
      601 ggtttgggtg cgctcggtcg agcacacctc atgcccacaa cgccagttca ccaaaggcgg
      661 ttgacagggg cttgtcagtc gccatggcgt cagaatttcg tcaactttcg tccgcgaaca
      721 ggggatgagc cagggcttca tccaggctca gcaccggcgt cacgcaggcg tcggcggcct
      781 cgaggaggtt cagccaatgc gccaacggcc gtgtgatcag caaggcctcg acttcgtcgc
      841 gcaaggccag cgcagcggcg ccgccgggcg cctggcccag cgaccaatgc tgctgcaccc
      901 aatccggccg cgcaagcgcg gtgcagaagt tgcgccagaa cttcagctcc agcgcgccca
      961 gcgcgaggta gcggccgtcg ccgcagcggt aaagcccgta acagggagcg ccgcctgata
     1021 gcaggtcgcg cccaggaggc ggcagctcgc cgcgggccgc aaggccagcg cgcagcacga
     1081 cctgatggcg cagcagctcg tggctcatcg agatgtcgat gtggcgccca cgcccgctgc
     1141 gctgcgcgcc tagcaacgcc gccagcaggg cgctgagcgc agcctgggtg ccgcccagca
     1201 ggtcgccgat ctggaaattg ggcagcgccg gctcgccctt gctggtcgcg atctgctgca
     1261 gcagcccggc catcgcgatg tagttgatgt catgcccggc gcgctgggcc caggggcctt
     1321 gctggccata gccgctgatc gagaccatca cgagccgggg attgacggcg ctcaaagtat
     1381 cccagcccag gcccagccta tccatcacgc cgggccggaa accctcgacc agcgcatccg
     1441 cctcgcgcac cagctcgagc agctcggcgc ggccagcctc gctgtgcagg tcgatgcggc
     1501 gcagctgctt gccggcgttc agttcccgga agaaggggcc cggctcgccg gaacgctcct
     1561 gggccgcgct gcgccatagc gcacgcgcgc cgtccccctc gccggggtct tcgatcttga
     1621 tgacctcggc gccccattgc gccagcctca gcgtcgcgag cgggcccggc agcaggcggg
     1681 tgagatcgac gatgcgcagg ccggccaggc aatccgcgtc gtccgcgtag tgcgggccgg
     1741 cgggagagct cattcgaagc ggcccacgcg cagccacttg gtcgccaccc atttctcgcc
     1801 ttcgacgacg ggcgcaccgc catgcagcgt gcgggtggtc gggtggggct tgtcgtagct
     1861 gaagaagact gcactgccct tgaccgccgc cacttccagc gccacgtcgg ggaaggtcgt
     1921 cccgccgccc ttgaccggat cgttgaggta gatcacgagc gtgcccacgc gctggccgcc
     1981 gcgcttgagg atgggtgccg agcccggctg ggccggatcg aaatagtcgt agtgcggctt
     2041 gtattcggcg ccggggcggt agtacaggat ctgcaggccc tcgccgtttt ccagcggcca
     2101 gttcaggagc gcggcgatgc gccgctcaac gcgcgcgatc agctcgttct ctgcgcggcg
     2161 gaagaacatg ccctcgctgg tgcgcgaggc gttgatctcg ctgccgccgg tggcggtgtc
     2221 caccgtctcg gagcgggaca ggcgctggcg cgccagcgcg acgatggcgt cgcattcctc
     2281 atcgctgagc aactcgccga acacgacgac gcgcggattg cgcatcgcca tcacgat
//
LOCUS       DASUCY010000675         2464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_160171, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000675 DASUCY010000000
VERSION     DASUCY010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160171"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VFV25_13350"
     CDS             <1..186
                     /locus_tag="VFV25_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HEY1394353.1"
                     /translation="CELQPASAGLADSEVREIAGWVAHRIKTLVGISTRVTVLKPDSI
                     ERTLVGKARRVIDKRPK"
     gene            209..1234
                     /gene="paaA"
                     /locus_tag="VFV25_13355"
     CDS             209..1234
                     /gene="paaA"
                     /locus_tag="VFV25_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009277109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HEY1394354.1"
                     /translation="MYTQAMDLMAKEGAKEGGDKKPHVRSAEEARLEERFEARIDAGD
                     FIEAKDWMPEHYRKTLVRQISQHAHSEIVGMLPEGNWITRAPSLKRKAILLAKVQDEG
                     GHGLYLYAAAETLGTSRDQMLDALHTGKAKYSSIFNYPTLSWADMGVIGWLVDGAAIM
                     NQVPLCRCSYAPYARAMIRICREESFHQRQGYEALLTMMTQGTSAQKAMVQDAVNRWW
                     WPSLMMFGPPDAESQHSAQSMRWGIKRLSNDELRQKFVDATVEQAKILGVTLPDPDLK
                     WNAEREHHDFGVIDWAEFWRVIGGDGPCNRERLTARVKAWEDGAWVREAAIAHAAKQA
                     DRSKAVA"
     gene            1256..1561
                     /gene="paaB"
                     /locus_tag="VFV25_13360"
     CDS             1256..1561
                     /gene="paaB"
                     /locus_tag="VFV25_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HEY1394355.1"
                     /translation="MTTNTETKREEWPLWEVFVRSKAGLDHKHCGSLHAPDAQLAIQL
                     ARDVYTRRQEGTSVWVVRSDQIVASDPATKDMYFDPMEDKVYRHPTFYVLPKAVDHM"
     gene            1587..2360
                     /gene="paaC"
                     /locus_tag="VFV25_13365"
     CDS             1587..2360
                     /gene="paaC"
                     /locus_tag="VFV25_13365"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HEY1394356.1"
                     /translation="MQSSIQIKNDAPVQYLLRIGDTCLILSQRLGEWCGHAPILEEDI
                     ALANMALDLLGQARAVLTHAGRLEGQGHDEDQLAFLREERDYLNPTIVELPRGDFAFT
                     VVRNAMVSTLLKLLWDKLHESSDVELAAIAGKAVKEARYHQQHAGEWLLRLGDGTDES
                     RRRTEAALQELWRFAGEFFESDAVDEQAAASKLGPRWADLREAWEAEMTALLGEADLA
                     VPQASAFRSTGKQGVHSEHMGYILTEMQHLQRAFPGGVW"
BASE COUNT          471 a          812 c          820 g          361 t
ORIGIN      
        1 tgcgagctgc agccggccag cgccggcctg gccgacagcg aggtgcgcga gatcgccggc
       61 tgggtggccc accgcatcaa gaccctggtc ggcatctcga ccagggtcac ggtgctcaag
      121 cccgattcca tcgagcgcac cttggtcggc aaggcgcgcc gcgtgatcga caagcgtccc
      181 aagtaaggcc ttgcaggaaa ggattgtcat gtatacccaa gcgatggacc tgatggccaa
      241 agagggggcc aaagagggcg gcgacaagaa gccgcacgtg cgctcggccg aggaagcccg
      301 gctcgaggag cgcttcgagg cgcgcatcga tgccggcgac ttcatcgagg ccaaggactg
      361 gatgcccgag cattaccgca agaccctggt gcgccagatc agccagcacg cgcactcgga
      421 gatcgtcggc atgctgcctg agggcaactg gatcacccgt gcgccctcgc tcaagcgcaa
      481 ggccatcctg ctggccaagg tgcaggacga gggcgggcac ggcctgtatc tctatgccgc
      541 ggccgagaca cttggcacct cgcgcgacca gatgctcgat gcgctgcaca ccggcaaggc
      601 caagtacagc tcgatcttca actacccgac gctgagctgg gccgacatgg gcgtgatcgg
      661 ctggctggtc gacggcgcgg ccatcatgaa ccaggtgccg ctgtgccgct gttcttacgc
      721 gccctacgca cgggccatga tccgcatctg ccgcgaggag agcttccacc agcgccaggg
      781 ctacgaggcc ttgctgacca tgatgacgca aggcacgtcg gcccagaagg ccatggtgca
      841 ggacgcggtg aaccgctggt ggtggccttc gctgatgatg ttcggcccgc ccgatgccga
      901 gtcgcagcac tcggcgcagt cgatgcgctg gggcatcaag cgcctgtcca acgatgagct
      961 gcgccagaag ttcgtcgacg ccaccgtcga gcaggccaag atcctcggcg tcacgctgcc
     1021 cgacccggat ctcaagtgga acgccgagcg cgagcatcac gacttcggcg tcatcgactg
     1081 ggccgagttc tggcgcgtga tcggcggcga cggcccctgc aaccgcgagc gcctgaccgc
     1141 ccgcgtcaag gcctgggaag acggtgcctg ggtgcgcgag gccgcgatcg cccatgccgc
     1201 caagcaggcc gaccgttcca aggccgtcgc ttaaagctag caaggaagca agatcatgac
     1261 gaccaatacc gaaaccaagc gcgaagaatg gccgctgtgg gaagtcttcg tgcgcagcaa
     1321 ggccggcctg gaccacaagc actgcggcag cctgcacgcg cccgatgcgc agttggcgat
     1381 acagctcgca cgcgacgtct atacgcgccg ccaggagggc accagcgtct gggtggtgcg
     1441 cagcgaccag atcgtggcca gcgaccccgc gaccaaggac atgtacttcg atccgatgga
     1501 agacaaggtc taccgccacc cgaccttcta cgtgctgccc aaggccgtgg accacatgtg
     1561 aagccgtgaa tgtgaaaaag gccgccatgc aatcctcgat tcagatcaag aacgatgcgc
     1621 ccgtgcaata cctgctgcgc atcggcgata cctgcctgat cctgtcgcag cgcctcggcg
     1681 aatggtgcgg ccatgcgccc atcctcgaag aggacatcgc gctggccaac atggcgctcg
     1741 acttgctcgg ccaggcgcgc gccgtgctga ctcatgccgg caggctggaa ggccagggcc
     1801 atgacgaaga tcagctcgcc ttcctgcgag aggagcgcga ctacctgaac ccgaccatcg
     1861 tcgagctgcc gcgcggcgac tttgccttca ccgtggtgcg caatgccatg gtgtccacgc
     1921 tgctcaaact gctatgggac aagctgcacg agtccagcga tgtcgagctc gcagccattg
     1981 ccggcaaggc ggtcaaggaa gcgcgctacc accagcagca cgcgggcgaa tggctgctgc
     2041 gcctgggcga cggtaccgac gaatcgcgcc gtcgcactga agcggccttg caggagctct
     2101 ggcgcttcgc cggcgaattc ttcgaatccg acgcggtcga cgagcaggct gccgcgagca
     2161 agctgggccc gcgctgggcc gatctgcggg aagcctggga agccgaaatg acggcgctgc
     2221 ttggcgaggc tgatcttgcc gtgccccagg ccagcgcctt ccgcagcacc ggcaaacagg
     2281 gcgtgcacag cgagcacatg ggctacatcc tgaccgagat gcaacacctg cagcgtgcct
     2341 tcccgggggg cgtgtggtga gtgcagtcat gcaagccagg gacgccgccg cgagcgaagc
     2401 gcgcgtggcg cgcgcctggg ccgtgctggc cgaggtgctg gaccccgagg tgccggcgct
     2461 ttcg
//
LOCUS       DASUCY010000676         2083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1492790, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000676 DASUCY010000000
VERSION     DASUCY010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492790"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VFV25_13370"
     CDS             complement(<1..183)
                     /locus_tag="VFV25_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1394357.1"
                     /translation="MSSNAQRAAARAAASRPAMEESWFGALGVGEDSQRDDAARGLDA
                     ESRYDGDWKAAQGRVAQ"
     gene            355..1926
                     /gene="ubiB"
                     /locus_tag="VFV25_13375"
     CDS             355..1926
                     /gene="ubiB"
                     /locus_tag="VFV25_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HEY1394358.1"
                     /translation="MRHLLRLAYIGFIVLRFGLDQLALHSLRRPWLRALARLLAVGRR
                     LDAPRGERLRQALERLGPIFVKFGQVLSTRRDLLPADIADELAKLQDRVPPFPGEVAR
                     RLVEEAYRKPIEAIFERFESEPVASASIAQVHFAVLKGGREVAVKVLRPGMLAAIEDD
                     LSLLRTAAGWIERYSEDGRRLRPREVVAEFDKYLHDELDLVREAANAAQLRRNMDDLK
                     LVLVPEMLWDLCTPGVIVMERMNGVPIGQVERLREAGIDIKKLARDGVTIFFTQVFRD
                     GFFHADMHPGNIQVSVEPASFGRYIALDFGIIGTLTQTDKDYLAQNFIAFFRRDYKRV
                     AELHVESGWVPRDTRVDELEGAVRAVCEPHFDRPLKDISLGQVLLRLFQASRRFNVEI
                     QPQLVLLQKTLLNVEGLGRQLDPELDLWSTAKPFLERWMNEQVGWRALLAQLRNEAPR
                     YAQLLPELPRLLHDALQARTLRGLSSAPDQLQLAALLAEQRRTNRLLQGVVYGGLGFL
                     IGLVLTLVMTRVHFF"
BASE COUNT          303 a          725 c          708 g          347 t
ORIGIN      
        1 ctgcgccacc ctgccctgcg cggccttcca gtcgccgtcg tagcgcgatt cggcatccag
       61 cccgcgtgcc gcatcgtcgc gctgcgaatc ctcccccacg cccagggcgc cgaaccagga
      121 ttcctccatc gccggcctgg acgccgcagc gcgcgccgcg gcacgctgtg cattgctgct
      181 catgaccacc cctcgtccga caagtacgtc ggctggtccc ccgaggggac cggcttggga
      241 ggtgagtgcc gcggctccaa gccgacctgc gtcggcttgg acggcacgaa cgcctgccga
      301 ctctggcggc cggcggcccg gcgctcggcc tgcgaaggac agattgactg tctcatgagg
      361 catctgctgc gcctagccta tatcggcttc atcgtgctgc gcttcgggct ggaccagctc
      421 gcgctgcaca gcctgcgcag gccctggctg cgcgcgctgg cgcgcctgct cgcagtgggg
      481 cgccggctcg atgcgccgcg cggcgagcgg ctgcgccagg cgctggagcg gctggggccc
      541 atcttcgtca agttcggcca ggtgctgtcc acgcggcgcg atctgctgcc ggccgacatc
      601 gccgacgagc tggccaagct gcaggatcgc gtgccgccct tcccgggcga ggtggcgcgc
      661 cgcctcgtcg aggaggccta ccgcaagccg atcgaagcga tcttcgagcg cttcgagtcc
      721 gagccggtgg ccagcgcgtc catcgcgcag gtgcatttcg cggtgctcaa gggcgggcgc
      781 gaggtggccg tcaaggtgct gcgcccgggc atgctggcgg cgatcgagga cgacctgagc
      841 ctgctgcgca ccgccgccgg ctggatcgag cgctattccg aggatggccg gcgcctgagg
      901 ccgcgcgagg tcgtggccga gttcgacaaa tacctgcacg acgagctgga cctggtgcgc
      961 gaggcggcca atgccgccca gctgcgccgc aacatggacg acctcaagct cgtgctggtg
     1021 cccgagatgt tgtgggacct gtgcacgccc ggcgtgatcg tgatggagcg catgaacggc
     1081 gtgcccatcg gccaggtcga gcggctgcgc gaggccggca tcgacatcaa gaagctggcg
     1141 cgcgacggcg tcacgatttt cttcacccag gtcttccgcg acggcttctt ccatgccgac
     1201 atgcacccgg gcaatatcca ggtcagtgtc gagccggcca gcttcgggcg ctacatcgcg
     1261 ctggacttcg gcatcatcgg cacgctgacg cagaccgaca aggactacct ggcgcagaac
     1321 ttcatcgcct tcttccgccg cgactacaag cgcgtcgccg agctgcacgt cgaaagcggc
     1381 tgggtgccgc gcgacacgcg ggtcgacgag ctcgaagggg cggtgcgcgc cgtctgcgag
     1441 ccgcacttcg accggccgct gaaggacatc tcgctgggcc aggtgctgct gcgcctgttc
     1501 caggcctcgc gccgcttcaa tgtcgagatc cagccccagc tggtgctgct gcagaagacc
     1561 ctgctcaatg tcgagggcct gggccgccag ctcgaccccg agctcgacct gtggagcacc
     1621 gccaagccct tcctcgagcg ctggatgaac gagcaggtcg gctggcgcgc gctgctcgcc
     1681 cagctgagga acgaggcgcc gcgctacgcc cagctgctgc ccgaactgcc gcgcctgctg
     1741 catgacgcgc tgcaagcccg cacgctacgt ggcctctcgt cggcgccgga ccagctgcag
     1801 ctggccgcgc tgctggccga gcagcgccgc accaaccggc tgctgcaggg cgtcgtctat
     1861 ggcgggctgg gcttcctgat cgggctggtg ctcacgctcg tgatgacgcg ggtgcatttc
     1921 ttctaggccg aagcgcgcct cgaggcgcat ggtgcagggc gttttgaccg tgctatcgaa
     1981 tcagtatcga tattcgccgt aaaacctagg gtagcttgaa gaattcttct agatttttca
     2041 tttgcctgcc gtgccggtac cgccgcagcg gcagggaatt gca
//
LOCUS       DASUCY010000677         4070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_192421, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000677 DASUCY010000000
VERSION     DASUCY010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192421"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VFV25_13380"
     CDS             <1..240
                     /locus_tag="VFV25_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEY1394359.1"
                     /translation="EDAVEILRCDLQLETAAQATIKDAIAHCETVRDYVSRDLLQDIL
                     DDTEEHIDFLETQLELVDQVGLQNYLQSQMGEASS"
     gene            complement(259..984)
                     /locus_tag="VFV25_13385"
     CDS             complement(259..984)
                     /locus_tag="VFV25_13385"
                     /EC_number="2.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF040696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-S-isoprenylcysteine O-methyltransferase"
                     /protein_id="HEY1394360.1"
                     /translation="MRVEVSSARARTNATAGVYGAKLRAWFPLVLFAFALAAYLWRRG
                     AQWQWPELAWFAAATLQMMIRWPHVERNRGNLTVEQRVSLGDQWAMLAVFLSLLCIPV
                     CYLATPWLDAWDYPLADGWSVLGALMMLVSLWVFHRSHVELGRNWSPSLEIRQGHSLV
                     TQGIYARVRHPMYASIWLFALAQPLLLHNWVAGALAIPGFGILYFLRVRAEEALMRET
                     FGEAYEAYMRRTGRLWPRFRARS"
     gene            complement(1144..2463)
                     /locus_tag="VFV25_13390"
     CDS             complement(1144..2463)
                     /locus_tag="VFV25_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007736101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1394361.1"
                     /translation="MAQLEGQRVPNRRWRIGALLGIGVLVNYLDRINLSVAAPQLKEA
                     FNLGPEQVGFFLSAFFWSYALLQVPAGVLLDRYGPTRIGRWGAFLWGIASTMTALAGG
                     FGGVFAARMLLGIAEAPGFPVSSKATGYWFPRKERAFATALFDAAAKFSNVIGVPLVA
                     VVVVSFGWRWGFGVTALLSFVYFLAFYFIYRDPSADKQLSETERRYIVEGGATPEGQA
                     SASAMGMLGYLLTKAKVWGLTIGFAAYGYSFYLFLTWLPGYLVQTMHMSILKSAGYAA
                     IPWMVATITDLFVGGWLIDHLVAKGHDESGVRKAVLIVGMVLGLAVFGATQTTDPVWA
                     IFWISIALGGLASAAPVGWSLPSLIAPKGGVGTVGGIMNFANNMMGAAAPIVTGFIVG
                     ATNSFTNAFIVAGVILLVGIVSFVFVLGRIEPIPDPSSSAQKSAALT"
     gene            complement(2545..3045)
                     /locus_tag="VFV25_13395"
     CDS             complement(2545..3045)
                     /locus_tag="VFV25_13395"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEY1394362.1"
                     /translation="MKSIVIMGVAGSGKSSLARAVAAEFGVPMVEGDDFHPPANLAKM
                     RSGTPLDDADRAGWLAALGAELQRLAGGAVLSCSALKRAYRASLREAVPDLKFVFLEI
                     DRASARERVATRAAEHLFPASLVDSQFAVLEPPMGEPGVLTLKATEEPGHLQALVRAW
                     IASGGS"
     gene            3228..>4070
                     /locus_tag="VFV25_13400"
     CDS             3228..>4070
                     /locus_tag="VFV25_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEY1394363.1"
                     /translation="MTSTTPAATDPPSTSRRRRSSGRVTLQDVAAAADVSPITASRAL
                     RGLASVAPELVARVKEAVDRLGYVPDPAARALASSRSTQVAVLVPMLSNALFVDLLEA
                     VHRTLFPAGYQPLIGVTHYDPQEEEELLRSYLTHRPAGLLVVGFDRTETSRQLIVGSG
                     LPCVHLMETTEAPGVHCVGFSQIEAGQAVTEHLLSRGRRRIAFAAAQLDPRTLQRAEG
                     YRRALRAAGCYDPHLELLNPDRSSLALGGRLLEELLKSRPDIDAIFFCNDDLAQGGLL
                     AAQRL"
BASE COUNT          742 a         1396 c         1295 g          637 t
ORIGIN      
        1 gaggatgcgg tggagatcct gcgctgcgac ctgcagctcg aaaccgccgc gcaggcgacg
       61 atcaaggacg ccatcgccca ctgcgaaacg gtgcgcgact atgtctcgcg cgatctgctg
      121 caagacatcc tcgacgacac cgaggagcac atcgatttct tggagaccca gctcgaactg
      181 gtcgaccagg tcggcctgca gaactatctg cagtcgcaaa tgggcgaagc ctcgtcctga
      241 acgagggagc tttgcgcgtc agctgcgggc ccgaaagcgc ggccacaggc ggccggtgcg
      301 ccgcatgtag gcctcgtagg cttcgccgaa ggtctcccgc atcagggcct cttcggcgcg
      361 caccctcagg aagtaaagga tgccgaagcc ggggatggcc agagcaccgg cgacccagtt
      421 gtgcagcaac aagggctggg ccagcgcgaa cagccagatc gaggcataca tggggtggcg
      481 tacgcgggcg tagatgccct gcgtgacgag gctgtgcccc tgcctgatct ccagcgacgg
      541 cgaccagttg cggccgagct cgacatggct gcgatggaag acccacagtg acaccagcat
      601 catcaaggcg cccagtaccg accatccgtc ggccagaggg tagtcccagg catccagcca
      661 gggcgtggcc aagtagcaaa cgggtatgca cagcaggctc agaaagacgg ccagcatggc
      721 ccattgatcg ccgaggctca cgcgctgctc taccgtgagg ttgccgcgat tgcgctccac
      781 gtgaggccag cggatcatca tctgcagggt cgccgcggca aaccaggcca actcgggcca
      841 ttgccattgc gcgccgcgcc gccacagata ggccgccaac gcgaaggcga acagcacgag
      901 cgggaaccat gcgcgcagct tcgccccgta gacgccggcc gtcgcattgg ttcgggcgcg
      961 cgccgatgac acctcgacgc gcatcaaggc cctgctcctc tacggcgcac cgcctgctgc
     1021 tttttctgcg ttggcaccac acatccaccc tggcatttgg gccgccgcgc ggaaatcgcg
     1081 cggcggcagt gcggtgaatg taggccctgg ggatcacctc gccagccccc cctgaagggg
     1141 gagttatgta agcgcggcgc tcttctgggc gcttgagctc gggtccggta tcggctcgat
     1201 cctgccaagc acgaagacga aggacacgat gcccaccagc aggatcacgc ccgcgacgat
     1261 gaaggcattg gtgaaggagt tggtcgcccc gacgatgaag ccggtcacga taggcgcggc
     1321 agcgcccatc atgttgttgg cgaagttcat gatgccgccc acggtgccga cgccgccctt
     1381 gggcgcgatc agcgagggca gcgaccagcc gaccggcgcc gccgacgcga ggccacccag
     1441 cgcgatcgag atccagaaga tcgcccacac cgggtcggtc gtctgcgtcg ctccgaagac
     1501 ggccagaccc aacaccatgc cgacgatcag cacggccttg cgcacgccgg attcgtcatg
     1561 gcccttcgcg accagatggt cgatcagcca gccgccgacg aagaggtcgg tgatggtggc
     1621 aaccatccac gggatcgcgg cataaccggc cgatttcagg atgctcatgt gcatggtctg
     1681 caccaggtag ccgggcagcc aggtcaggaa gaggtagaag gaatagccgt aggcggcgaa
     1741 gccgatcgtc agcccccaga ccttggcctt ggtcagcaga tagcccagca tgcccatcgc
     1801 gctggcgctg gcctgtccct cgggcgtcgc gccgccctcg acgatgtagc ggcgctcggt
     1861 ctcactcagc tgcttgtccg cgctcggatc gcgatagatg aaatagaacg ccaggaaata
     1921 aacgaagctc agcaaagccg tgacgccgaa gccccagcgc cagccgaagc tgacgacgac
     1981 caccgcgacc agcggcacgc cgatgacatt ggaaaacttg gccgcggcgt cgaacagggc
     2041 cgtggcgaag gcacgctcct tgcgcggaaa ccaataaccg gtggccttgg agctgaccgg
     2101 gaagcccggc gcctcggcga tgcccaacag catgcgcgcg gcgaacacgc cgccgaagcc
     2161 gcccgctagc gccgtcatcg tggaagcgat accccacaga aaggcgcccc agcggccgat
     2221 gcgcgtcgga ccgtagcggt cgagcagcac gcctgccggc acctgcagca gggcgtagga
     2281 ccagaagaag gcgctgagga agaagccgac ctgctcgggc cctaagttga aggcttcctt
     2341 cagctgcggc gccgcgaccg acaggttgat gcggtccagg tagttgacga gcacgcctat
     2401 gcccagcagc gcgcctatcc tccagcggcg gttgggtact ctctgtcctt ccagttgcgc
     2461 catgcttggt ctccgtggtg gttggtcttt ggggtgtgcg ccgatctcgc ggggcgccgc
     2521 tctatcgact tctcgcgctg cagctcatga gccgccgctt gcgatccagg cccgcacaag
     2581 ggcctgcaga tgcccgggtt cctcggtcgc tttcagggtc agcacgccgg gctcgcccat
     2641 gggcggctcc aggaccgcga actggctgtc gaccaggctg gcaggaaaga gatgctcggc
     2701 ggcccgcgtc gcaacgcgct cgcgcgccga ggcgcggtcg atctcgagaa agacgaactt
     2761 caggtccggc accgcctcgc gcaagctggc gcgataggcg cgcttgagcg ccgagcagga
     2821 caggacggca ccgcctgcga gccgctgcag ctcggcgcca agggccgcga gccagcccgc
     2881 gcggtcggca tcgtcgagtg gagtgccgct gcgcatcttg gccaggttcg cgggcgggtg
     2941 aaaatcatcg ccctccacca tcggcacgcc gaattcagcg gccacggctc gtgccagact
     3001 ggacttgcca ctgccggcga cgcccatgat cacgatcgac ttcattgcat gacccttgga
     3061 agcttggttt gcgcctgagt tagcgctatc tcgatcaacc cggcaccgtg atctctcatt
     3121 caaattgtta gccggagcgg attacgatcg acttcaggtt ttcacctatg ctgagatagc
     3181 gctatcctag tcgatacttt cagccaccac attccgggat ttcccgcatg acctcgacga
     3241 cgcctgccgc gaccgatcct ccttccacct cccgacgtcg ccgctcgagc ggccgggtga
     3301 cgctgcagga cgtggccgcc gcagccgacg tgagtccgat caccgcttcc cgcgcacttc
     3361 gggggctggc cagcgtcgcc cccgaactcg tcgcccgggt caaggaggcc gtcgaccggc
     3421 tcggctatgt gcccgacccc gctgcgcgag ccctggcgtc ctcgcgcagc acccaggtcg
     3481 ccgtgctcgt tcccatgctg tcgaacgcgc tcttcgtcga tctgctcgaa gcggtgcatc
     3541 gcaccctctt cccggcgggc taccaaccgc tgatcggcgt gacccattac gacccccagg
     3601 aagaggaaga gctgctgcgc agctatctca cgcatcgccc ggccggtctg ctcgtcgtcg
     3661 gcttcgaccg caccgaaacc agccgccagc tcattgtcgg cagtggcctg ccctgcgtcc
     3721 acctgatgga gaccaccgaa gcaccgggcg tgcactgcgt cggcttttcc cagatcgaag
     3781 ccggccaggc cgtgaccgag cacctgctga gccgcggacg ccggcgcatc gcctttgccg
     3841 ccgcacaact cgatccacgc acactgcagc gcgcggaagg ctatcggcgt gcgctgcgtg
     3901 cagcaggctg ctacgacccg cacttggagc tgctcaatcc cgaccgctcc tcgctagccc
     3961 tgggcggacg cctgctcgag gaattgctga aatcgcgacc ggacatcgat gcgatcttct
     4021 tctgcaacga cgaccttgcc caaggcggcc tgcttgcggc tcagcgcctg
//
LOCUS       DASUCY010000678         2071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_481397, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000678 DASUCY010000000
VERSION     DASUCY010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481397"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="VFV25_13405"
     CDS             <1..799
                     /locus_tag="VFV25_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6537 domain-containing protein"
                     /protein_id="HEY1394364.1"
                     /translation="ALMRAIELNGVQADNNKAAFEWGRRCAHDLASVKALFKAAQVIE
                     FVKKPSVAETIAKRADFLTGYQNAAYAADYRAFVEKVRAAEAKLDNKGTRLSEAVARY
                     LFKLMAYKDEYEVARLHTDRAFTDKIAAMFEGDYKLVHHLAPPLTAKRNDKGELVKRP
                     FGPWMRSAFGLLAKMKGLRGTALDVFGRSEERKTERALIVEYRACIDELLSGLNAERL
                     ALAVEIASLPEEIRGYGHVKERHLKAAREKWARLMAQWRAGAQAEAA"
     gene            996..1592
                     /locus_tag="VFV25_13410"
     CDS             996..1592
                     /locus_tag="VFV25_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEY1394365.1"
                     /translation="MHSSLSAAAPLSPAILPKSARYGATAIALHWLLALAIVATFGVG
                     LYMADLPMSPLRLKLFNWHKWAGTTILALSALRLLWRLLRRPPPLPEGIAANMPGWQH
                     LAHSATHGLMYLLFFIVPLVGWAYTSATGFPVVVFGVLPLPDFVPVDKALAEAIKPWH
                     EITAWALAVLVMLHMAGALKHHFFDRDGLLGRMWPVRG"
     gene            1631..>2071
                     /locus_tag="VFV25_13415"
     CDS             1631..>2071
                     /locus_tag="VFV25_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEY1394366.1"
                     /translation="MKVTLSTTFAAIACAGWLSFAATPASAQTPATLVPAQSEIAFVS
                     KQMGVPVEGKFKKFDAQISLDPKKPESGKVAFNIALGSASLGSPETEAELVKPAWFDA
                     AKFPQASFQSSAIKGLGGGKFQVSGKLSIKGNAHDVVVPVTLTQS"
BASE COUNT          363 a          725 c          647 g          336 t
ORIGIN      
        1 cgcgctgatg cgcgccatcg aactcaacgg cgtgcaggcc gacaacaaca aggccgcctt
       61 cgaatggggc cgccgctgcg cacacgacct cgcatctgtg aaggcgctgt tcaaagcggc
      121 ccaggtgatc gagttcgtca agaagccctc cgtggccgag acgatcgcca agcgtgcgga
      181 cttcctcacc ggctaccaga acgccgccta tgcggccgac tatcgggcct tcgtcgagaa
      241 ggtgcgcgcg gccgaggcga agctcgacaa caagggcacg aggctgagcg aggcggtggc
      301 acgctacctc ttcaagctga tggcctacaa ggacgagtac gaagtcgcgc gcctgcacac
      361 cgaccgcgcc ttcaccgaca agatcgcggc catgttcgag ggcgactaca agctcgtgca
      421 ccacctcgcg ccgccgctca cggccaagcg caacgacaag ggcgagctgg tcaagcggcc
      481 cttcggcccc tggatgcgtt cagccttcgg cctgctcgcc aagatgaagg gcctgcgcgg
      541 cacggccctc gacgtcttcg gccgcagcga ggagcgcaag accgagcgcg cgctgatcgt
      601 cgaataccgc gcctgcatcg acgagctgct gagcggcctg aacgccgagc gcctggccct
      661 tgcggtggag atcgccagcc tgccggaaga gatccgcggc tatggccatg tgaaggaacg
      721 ccacctgaag gccgcgcgcg agaaatgggc gcggctgatg gcccaatggc gcgcaggcgc
      781 tcaggccgag gcagcctgaa caaacgctga catcgccctt cgctcgtcag ttcgctcatg
      841 agcctcgctc ggccgcgcgc cgagcggggc tggcaatgtt caattccttt gaatcgctgg
      901 ctcaagccgg ctgaacttcg gttcgcctgc ttcgaactaa tctctcgccg tccgccattc
      961 accgggcgga gcgcagctgt taccagtcga ttcaaatgca ttcaagctta agcgccgccg
     1021 cgcctctctc gcccgcgatc ctgcccaaga gcgcccgcta cggcgccacg gccatcgccc
     1081 tgcactggct gctcgcgctg gccatcgtcg cgaccttcgg cgtggggctc tacatggccg
     1141 atctgccgat gtcgccgctg cgcttgaagc tcttcaactg gcacaaatgg gcgggcacca
     1201 ccatcctcgc gctgtcggcg ctgcgcctgc tgtggcgttt gctgcgccgc ccgccgccgc
     1261 tgcccgaggg catcgcagcc aacatgccgg gatggcagca cctcgcgcac agcgcgacgc
     1321 atgggctgat gtatctcctc ttcttcatcg tgccgctggt gggctgggcc tacacctcgg
     1381 caacgggctt ccccgtcgtc gtgttcggcg tcctgccgct gcccgacttc gtgccggtgg
     1441 acaaggccct ggccgaagcc atcaagccct ggcacgagat caccgcctgg gcgctcgccg
     1501 tgctggtcat gctgcacatg gccggcgctc tcaagcatca cttcttcgac cgcgacggcc
     1561 tgctgggccg catgtggccg gtccgcggct aaaacgcggt ccgcccaaga acacgaacag
     1621 gaaaccaccc atgaaagtca ctttgtccac gacctttgcg gccatcgcct gtgccggctg
     1681 gctgagcttt gctgcgacgc cggccagcgc tcaaacgccc gccacgctgg tgcctgcgca
     1741 aagcgagatt gctttcgtca gcaagcagat gggcgtgccg gtggaaggca agttcaagaa
     1801 gttcgacgcc cagatcagcc tcgaccccaa gaagcccgag agcggcaagg tcgccttcaa
     1861 catcgcgctt ggcagcgcca gcctgggctc gcccgagacc gaggccgagc tggtgaagcc
     1921 ggcctggttt gacgccgcca agtttcctca ggccagtttc cagtccagcg ccatcaaggg
     1981 cctgggcggc ggcaagttcc aggtcagcgg caagctcagc atcaagggca acgcgcatga
     2041 cgtggtcgtg ccggtcacgc tgacccagtc c
//
LOCUS       DASUCY010000679         2206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_818359, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000679 DASUCY010000000
VERSION     DASUCY010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818359"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..1656
                     /gene="glpD"
                     /locus_tag="VFV25_13420"
     CDS             76..1656
                     /gene="glpD"
                     /locus_tag="VFV25_13420"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HEY1394367.1"
                     /translation="MLPQTLTLPAAQAEIDCDVLVVGGGINGAGIARDLAGRDWRVLL
                     CERDDLASHTSSASTKLIHGGLRYLEYYEFGLVRKALMEREVLLRSAPHIMKPLRFVM
                     PHDPTMRPAWMIRLGIFLYDHLARRELLPGSRGVDLSRSELGAPLKPEYRRGFIYSDG
                     WVDDARLVVLNAVDATERGATVLTRTRCIAAQRHAQGWTATLLDREGLCHKVNARALV
                     NAAGPWAESFLREIAQPAHGETLATKSLRLVKGSHIVVPRCFEHDHAYIFQNPDKRII
                     FAIPYEGEFTLIGTTDVELRGDPGAACIDADETAYLCEQASRYFKQQIGPSDVVWSYA
                     GVRPLLDDDSGDPSAVTRDYLLETHFRGGAPLLSVWGGKITTFRKLAEEAADEIGSML
                     GEARKAWTQGATLPGGDFSGWISGAARRSDTGFERFLAELQRRHAWLPAALARRWAHA
                     YGTRVELLLAGAKSLADLGTEIAPGLHEAELRYLQREEWALAAEDVLWRRSKLGLHYS
                     QAQREAVRDWLEAGTGRG"
     gene            1769..>2206
                     /locus_tag="VFV25_13425"
     CDS             1769..>2206
                     /locus_tag="VFV25_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1394368.1"
                     /translation="MKQQPGFPLARLLALTAAAALSACGGGYSSSFNYSADLADPAIA
                     SQLSSIEAVGVGGAGLLSGDPAFKFKALRANLKGGSAQVRFNCPTCNFTITAGGASVG
                     ANQFLTVNFPTLDSSSDVAITVKDKVSGVSTVYTLRAAPRDHPP"
BASE COUNT          351 a          764 c          738 g          353 t
ORIGIN      
        1 aaagtccatg gcctcgctta cccgcagcga acgccggccg tccatgagga gcccccgttg
       61 accacccagc cgccggtgct gccgcaaacc ctgaccctgc cagccgcgca agccgagatc
      121 gattgcgacg tcctggtcgt cggcggcggc atcaatggcg ccggcatcgc acgcgacctg
      181 gccggacgcg actggcgcgt gctgctgtgc gagcgcgacg acctggcctc gcatacctcg
      241 tcggcctcga ccaagctgat tcatggcggc ctgcgctatc tcgagtacta cgagttcggc
      301 ctggtgcgca aggcgctgat ggagcgcgag gtgctgctgc gcagcgcgcc gcacatcatg
      361 aagccgctgc gcttcgtgat gccgcatgac cccacgatgc gtccagcatg gatgatccgg
      421 ctcggcatct ttctctatga ccatctggcg cggcgcgagc tgctgccggg ctcgcgcggc
      481 gtggacttga gccgcagcga gctgggcgca ccgctcaagc cagagtatcg gcgcggcttc
      541 atctattcgg acggctgggt cgacgacgcg cggctggtcg tgctcaatgc tgtcgacgcg
      601 acggaacgcg gcgcgaccgt gctgacgcgc acccgctgca tcgccgcgca gcgccatgcc
      661 cagggctgga ccgcgacgct gctggaccgc gaaggccttt gccacaaggt gaacgcacgt
      721 gcgctggtca acgcagccgg tccctgggcc gagagcttcc tgcgcgaaat cgctcagcct
      781 gcccatggcg aaacgcttgc caccaagagc ctgcgcctgg tcaagggcag ccacatcgtc
      841 gtgccgcgct gtttcgagca cgaccatgcc tacatcttcc agaacccgga caagcgcatc
      901 atcttcgcca tcccctacga aggcgagttc acgctgatcg gcaccaccga tgtcgagctg
      961 cgcggcgacc ctggggccgc ctgcatagac gccgatgaaa cggcctactt atgcgagcag
     1021 gcgagccgct atttcaagca acagatcggg ccctccgatg ttgtatggag ctatgccggc
     1081 gtgcgaccgc tgctggacga cgactcgggt gatccctcgg ccgtcacgcg cgactatctg
     1141 ctcgaaaccc acttccgcgg cggtgcccct ctgctcagcg tctggggcgg caagatcacg
     1201 accttccgca agctggccga ggaggccgcc gacgagatag ggtccatgct gggcgaagcg
     1261 cgcaaggcct ggacgcaggg cgccacgctg ccgggcggcg acttctcggg ctggatcagt
     1321 ggcgcggcgc ggcgctcgga caccggcttc gagcgcttcc ttgccgagtt gcagcggcgc
     1381 catgcctggc tgcctgccgc gctggcgcgg cgctgggccc atgcctacgg cacgcgcgtc
     1441 gagctgctgc tggccggggc caaatcgctg gccgatctgg gcaccgagat cgcacccggc
     1501 ctgcatgagg ccgagctgcg ctatctgcag cgcgaggaat gggcgctcgc cgccgaggat
     1561 gtgctgtggc ggcgttccaa gctgggcctg cattacagcc aggcgcagcg agaggcggtg
     1621 cgggactggc tggaagccgg caccgggcgc ggctagatcg gcggcatgct gcgcacgccc
     1681 gtcgcccgag cagaaacatt gaaaccgcgc ttctcatcgc cggcagtcag gcgcatattt
     1741 gttgttcgtc tggcatagga ccacggccat gaagcagcag cccggattcc ccttggcgcg
     1801 cctgctcgcg ctgactgccg ctgccgcact gagcgcgtgc ggcggcgggt attcgtcgtc
     1861 tttcaactac agcgcggacc tcgccgatcc ggcgatcgca tcccagctga gctccatcga
     1921 ggccgtcggc gtcggcggtg cgggcctcct gtccggcgac ccggcattca agttcaaggc
     1981 gctgcgggcc aatctgaagg gcggcagcgc tcaggtccgc ttcaactgcc cgacctgcaa
     2041 cttcaccatc acggcgggtg gggcgagcgt gggtgccaac cagttcctga cggtgaactt
     2101 cccgacgctg gacagcagct cggacgtggc catcacggtc aaggacaagg tgagcggcgt
     2161 gagcacggtc tacacgctgc gtgccgcgcc gcgcgaccac ccgccc
//
LOCUS       DASUCY010000680         4487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1445188, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000680 DASUCY010000000
VERSION     DASUCY010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4487
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1445188"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VFV25_13430"
     CDS             <1..412
                     /locus_tag="VFV25_13430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1394369.1"
                     /translation="KQLGAWFPAQSLMMAPSYIEKHPDTVQHLVNAFVRTMRFINSHS
                     VDEIVAKLPADYFKGKDRKAEIQLIRNTISTQAKGSYAFSPEAVQMVLDMNNGSAFDK
                     SDEGQWRAGGDKSKAVVDQLYTNRFVDAAMRAIR"
     gene            489..1499
                     /locus_tag="VFV25_13435"
     CDS             489..1499
                     /locus_tag="VFV25_13435"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY1394370.1"
                     /translation="MLALMQSGTQAAELDPSLPTYRPQPVEIPADRHFVLADGTIRIV
                     AGNRGMGVVIEGFNRLFEATHPGTKFKVEYNAGGNTVNIAALVHDTTLFSPLGRDANA
                     FERASYANIVGGSPLTIRIAHGTLSSPRMTAGLAIYVNERNPVRSLTMAQLARIFTTG
                     APGGDLTTWGQLGATGEWASQRIRTFGTPEASGYGDYMVNAKWDGRPFAPGYESYDLA
                     AQIVERVGAEPSGIGFAGMGFLTPQTHLIALAEQDGGPYLTGTEEEIASGRYPLDRTV
                     ALAIRRAAGQPVPALIKEYLRLVLSKEGQAIVAAEQDGFVPLTAEQARAELAKLEEGA
                     QP"
     gene            1496..2473
                     /locus_tag="VFV25_13440"
     CDS             1496..2473
                     /locus_tag="VFV25_13440"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY1394371.1"
                     /translation="MNLTRRLCLAAGLAVLAGRSGAAQVLSIFGDETLQPVLAPLNAL
                     FEAAHPGVRIGMRLKPPPAGIDGIVAGVSMLAPLAHDATEGELEPYKRLFGHLPIDVR
                     IGRIGFRAPGRKSPPAVVVHADNPLRSLSMSELARVLTTGQAGGDLRRWNQLGVDAGP
                     WRAHAIHLYGTHDNGGYVTALRASQFGNKPLAAHYEALAQDQDVLDAVSADRYALGIA
                     SQGVDDSELGGARMLALSSGALAASTATLDEVRQGRYPLSPFLHIYLPARSRAEIAAP
                     AREYVRLALSRQGQQLIGAGASNPAGLVPLPPHELAAERAKVDEPQARP"
     gene            2601..3809
                     /locus_tag="VFV25_13445"
     CDS             2601..3809
                     /locus_tag="VFV25_13445"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEY1394372.1"
                     /translation="MNRYLLHRGTRALTTLASSVLALAAGLHGGSASAQAQSEDAKNA
                     IRAQTDRALVIYKRGKTAHYKDSFDLSGMPHYAPSQQFTGWIRLHGNNYLADGMLGEY
                     WEQGFQKYQPGVRFSNYQPTSALAFAALYYNQADLVMGHRPGFYDLLAYERVMAYDPT
                     EITAVTGSFDVGGWENSITILVNEDNPLKCINMAQLDAIFGSERTGGWAGTNWRADWA
                     RGPEKNIRTWGQLGLTGEWADKPIGVYGFSIRYNTATDFADKVLKGSDKWNEKIQAYG
                     NIVKPDGTRYVEADQITDNLAKDRFGIAYNRYRGERPHVRRLAVAPGNSSNCVEHTLK
                     NVQNRTYPLFNEQYFYINVKPGTKMDPMVKEFLRYVLSQEGQADIIRDGKYLPLTAEV
                     VREQLKKLDQ"
     gene            3829..>4487
                     /locus_tag="VFV25_13450"
     CDS             3829..>4487
                     /locus_tag="VFV25_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1394373.1"
                     /translation="MKRRELLISALASAAAGTAIAAEKEKDSVEVQAAREAWVKAKGY
                     RVYYTKRWDLSDLPAYQPEQKVSGTIRLWGSNYFTDSPLAEWWAKEFQKFHPDAKFDF
                     HLKTTLHAIPGLIMGVSDIGPMGRQITFPEMLSFQREFNRMPTGIVAVTGSYNVSGWN
                     PPVGVYLNSKNPLAQLSLEQLDGIFGAERSGAWRDLTWDKSLARGPEKNIRTWGALGL
                     TG"
BASE COUNT          818 a         1606 c         1427 g          636 t
ORIGIN      
        1 gaagcagctc ggcgcctggt tcccggcgca gtcgctgatg atggcgccga gctatatcga
       61 gaagcacccg gacacggtgc agcacctggt gaacgccttc gtgcgcacca tgcgcttcat
      121 caacagccac tcggtggacg agatcgtcgc caagctgccg gccgactact tcaagggcaa
      181 ggaccgcaag gccgagatcc agctgatccg caacaccatc tccacccagg ccaagggcag
      241 ctatgccttc tcgcccgagg ccgtgcagat ggtgctggac atgaacaacg gctccgcctt
      301 cgacaagagc gacgagggcc aatggcgcgc cggcggcgac aagagcaagg ccgtggtcga
      361 tcagctctac accaaccgct tcgtcgatgc cgcgatgagg gcgatccgct gaatgcccca
      421 ggcatcgggg acccatcgtg aatgcgggct cgcatcaagg agtgacgcgt gccgcggctg
      481 cgctgctcgt gcttgcgctg atgcagtccg ggacgcaggc cgcggagctc gaccccagcc
      541 tccccaccta tcgtccgcag ccggtcgaga ttcccgcgga ccggcacttc gtgctcgccg
      601 atggaaccat ccgcatcgtc gcgggcaatc gtggcatggg cgtcgtcatc gaaggcttca
      661 accgcctgtt cgaagccacc cacccgggca cgaagttcaa ggtcgagtac aacgccggcg
      721 gcaacaccgt caacatcgcg gccctggtgc atgacacgac cttgttcagt cctttgggcc
      781 gtgacgccaa cgccttcgag cgggccagct acgcgaacat cgtcggcggc tcgccgctga
      841 ccatccggat cgcgcacggc acgctgagtt cgccgcgcat gaccgccggc ctcgccatct
      901 acgtcaacga gcgcaacccg gtgcgcagcc tgacgatggc gcaactggcg cgcatcttca
      961 cgaccggcgc tccgggcggc gatctgacca cctgggggca gctcggcgcc accggcgaat
     1021 gggcgtcgca gcgcatccgc accttcggta cgccggaggc cagcggctac ggcgactaca
     1081 tggtcaacgc aaaatgggat ggtcgcccct tcgccccggg ctacgaatcc tacgacctgg
     1141 cggcgcaaat cgtcgaacgc gtcggcgccg agcccagcgg catcggcttt gcgggcatgg
     1201 gcttcctcac gccgcagacg cacctcatag cgctcgccga gcaggacggc gggccctacc
     1261 tcaccggcac ggaagaggag atcgcgagcg gccgctatcc gctcgaccgg accgtcgcgc
     1321 tggcgatccg gcgcgcggcc ggccagcctg tgcccgcgct gatcaaggag tatttgcggc
     1381 tggtgctgtc gaaggaaggc caggccatcg tcgccgcgga gcaggacggc ttcgtgcctc
     1441 tcaccgccga gcaggcacgc gccgagctgg cgaagctgga agaaggcgca caaccatgaa
     1501 tctcacgcga cgactatgcc tggccgccgg ccttgcggtc ctggccggcc gttcaggcgc
     1561 ggcgcaagtc ctgagcatct tcggcgacga gaccctgcag ccggtactgg cgccgctcaa
     1621 cgcgctgttc gaggccgccc atccgggcgt gcgcatcggc atgcggctca agcccccgcc
     1681 ggccggcatc gacggcatcg tcgccggcgt gtccatgctc gcgccgctgg cgcacgacgc
     1741 gaccgaaggc gagctggaac cgtacaagcg cctcttcggc catctgccca tcgatgtgcg
     1801 catcggccgc atcggttttc gcgcgcccgg ccgcaaatcg ccgccggccg tggtcgtcca
     1861 tgccgacaac ccgctgcgca gcctctcgat gtcggagctg gcccgggtgc tgacgaccgg
     1921 gcaggccggc ggcgacctgc gccggtggaa tcagctcggt gtcgacgcag gcccttggcg
     1981 ggcgcacgcc atccacctct acggcacgca cgacaacggc ggctacgtta ccgcgctgcg
     2041 cgcctcgcag ttcggcaaca agccgctggc cgcgcattac gaagcgctgg cgcaggatca
     2101 ggatgtgctc gacgcggtgt ccgccgaccg ctacgcgctg ggcattgcca gccagggcgt
     2161 cgacgacagc gagctgggcg gcgcccgcat gctggcgctg tccagcggcg cgctggctgc
     2221 gtccactgcc acgctcgacg aggtgcggca aggccgctat cccctgtcgc ccttcctgca
     2281 catctacctg ccggcccgca gccgcgccga gatcgcagcg ccggcgcgcg aatacgtgcg
     2341 cctggcgcta tcaaggcaag gccagcagct gatcggcgcg ggggcctcga accccgccgg
     2401 cctcgtgccg ctgccgccgc acgagctcgc cgcagaacgg gccaaggtcg acgagccgca
     2461 agcaaggcca tgacgacccc tcgcccgacg agtacgtcgg ccggtccccc gaggggacgc
     2521 gggccggctt gggacccggc gctcggcccg tcgacgcctt caacatcgtt ccgaacccgc
     2581 acagcgatcc aggagacacc atgaatcgat acctccttca ccgcggcacc cgtgcgctga
     2641 cgacgctggc gagcagcgtt ctcgcgctcg ccgccggcct gcacggcggc agcgcgtccg
     2701 cgcaggccca atccgaagac gccaagaacg cgatccgcgc gcagaccgac cgtgccctgg
     2761 tcatctacaa gcgcggcaag acggcgcatt acaaggacag cttcgacctg agcggcatgc
     2821 cgcactacgc gccgtctcag cagttcaccg gctggatccg cctgcatggc aacaactacc
     2881 tggccgacgg catgctgggc gagtactggg agcagggctt ccagaagtac cagccgggcg
     2941 tgcgcttctc caactaccag cccacctcgg ccctggcctt cgcggcgctc tactacaacc
     3001 aggccgacct ggtgatgggc caccgccccg gcttctacga cctgctggcc tacgagcgcg
     3061 tgatggccta cgacccgacc gagatcaccg ccgtcaccgg ctccttcgac gtgggcggct
     3121 gggagaactc gatcaccatc ctcgtcaacg aggacaaccc gctcaagtgc atcaacatgg
     3181 cccagctcga cgccatattc ggctccgagc gcacgggcgg ctgggccggc accaactggc
     3241 gcgccgactg ggcccgcggc cccgagaaga acatccgcac ctggggacag ctgggcctca
     3301 ccggcgaatg ggccgacaag cccatcggcg tctacggctt cagcatccgc tacaacaccg
     3361 cgaccgactt tgccgacaag gtgctcaagg gcagcgacaa gtggaacgag aaaatccagg
     3421 cctacggcaa tatcgtcaag ccggacggca cgcgctatgt ggaggccgac cagatcaccg
     3481 acaacctggc caaggacagg ttcggcatcg cctacaaccg ctaccgcggt gagcgccccc
     3541 atgtgcggcg tttggccgtg gcgccgggca acagcagcaa ctgcgtggag cacacgctca
     3601 agaacgtcca gaaccggacc tacccgctgt tcaacgagca gtacttctac atcaacgtga
     3661 agccgggcac caagatggac ccgatggtca aggagttctt gcgctacgtg ctgagccagg
     3721 agggtcaggc cgacatcatc cgcgacggca aatacctgcc gctgacggcc gaggtggtgc
     3781 gcgagcagct gaagaagctc gatcaataag acacacggag acttgaagat gaaacgtcgt
     3841 gaactgctga tctctgcgct ggccagcgcc gctgccggca ccgccatcgc cgccgagaag
     3901 gagaaagact ccgtcgaggt gcaggccgcg cgcgaagcct gggtcaaggc caagggctac
     3961 cgcgtctact acaccaagcg ctgggacctg agcgacctgc cggcctacca gcccgagcag
     4021 aaggtcagcg gcaccatccg cctctggggc tccaactact tcaccgacag cccgctggcc
     4081 gaatggtggg ccaaggagtt ccagaagttt catccggatg cgaagttcga cttccacctc
     4141 aagaccacgc tgcacgccat cccaggcttg atcatgggcg tgtcggacat cgggcccatg
     4201 ggccggcaga tcaccttccc ggagatgctg tccttccagc gcgagttcaa ccgcatgccc
     4261 acgggcatcg tcgccgtcac cggcagctac aacgtctcag gctggaaccc gccggtgggt
     4321 gtgtacctga acagcaagaa cccgctggcc cagctctcgc tggagcagct ggacggcatc
     4381 ttcggcgccg agcgctccgg cgcctggcgc gacctcacct gggacaagtc cctggcccgc
     4441 ggcccggaga agaacatccg cacctggggg gctttgggcc tgaccgg
//
LOCUS       DASUCY010000681         2182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1573526, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000681 DASUCY010000000
VERSION     DASUCY010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1573526"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VFV25_13455"
     CDS             <1..469
                     /locus_tag="VFV25_13455"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEY1394374.1"
                     /translation="FQQQAKIKLVHREFNGSGEAVKAVQSGKVDLMFTALPSALKSLK
                     EGKLRALALSSDRRLALLPEVETFRENGMRGYLVASWFGLMVPKDTPAAVIERLNSEV
                     RAALATPQVRQQLADNGGSSSRMSVAEAADFFRTDTQIWGAVTTMAGIKPSGR"
     gene            complement(480..1571)
                     /locus_tag="VFV25_13460"
     CDS             complement(480..1571)
                     /locus_tag="VFV25_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HEY1394375.1"
                     /translation="MTKLFDPIRLGDITLANRIVMAPLTRNRAGAGQVPTELMVEYYR
                     QRAHPRDGAGLIVTEASQISPEGQGYLDTPGIYSAEQIAGWKKVTDAVHARGGKIVIQ
                     LWHVGRVSHVSLQPGGAAPLAPSAIRANTKTYVRGGFEPVSEPRALELHEIPAIVAQY
                     RDAARHAMQAGFDGVEVHGANGYLIDQFLRDGSNHRTDAYGGSIENRARFLLEVMRAV
                     TTEIGRARTGLRLSPVTPANDARDSNPQPLFNYAAEQLAPLELMYVHVIEGQTGGARD
                     IAPFDYQALRRRLRTPWMVNNGYDRAMAMQAVESGEADLVAIGRPFISNPDLVRRLRE
                     GAPLNAPNSKTFYGGGAEGYTDYPTLEAA"
     gene            complement(1676..>2182)
                     /locus_tag="VFV25_13465"
     CDS             complement(1676..>2182)
                     /locus_tag="VFV25_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide transporter"
                     /protein_id="HEY1394376.1"
                     /translation="LDQTTMSAGQQPANWSSAMTYKFVGALRSLTDDKPYQRTAIWVG
                     VLIGLLLEVARKLLRASESWRRWVQGSARVGFSADFVMDAFILPSPYAFSFGAFLSLA
                     TSSWFAAGGILSSALNSLKRPGKDDDGGGLPSDMSTTSLVGGGLIAGDALAALGLGVA
                     GLLATVLG"
BASE COUNT          342 a          722 c          772 g          346 t
ORIGIN      
        1 gttccagcag caggccaaga tcaagctcgt gcaccgcgag ttcaacggct cgggcgaggc
       61 ggtcaaggcc gtgcagtcgg gcaaggtgga cctgatgttc acggccctgc cctcggcgct
      121 caagtcgctg aaggagggca agctgcgcgc gctggccctc agcagcgacc gccgcctggc
      181 gctcctgcct gaggtggaga cctttcggga gaatgggatg cgcggctatc tggtcgcgag
      241 ctggttcggc ctgatggttc ccaaggacac gccggccgcg gtgatcgagc gcctgaacag
      301 cgaggtcagg gccgcgctcg ccacgcccca ggtgcgccaa cagctggccg acaacggcgg
      361 cagctccagc cgcatgagcg tcgccgaggc ggccgatttc ttccgcaccg atacccagat
      421 ctggggcgct gtgacgacca tggcgggcat caagcccagc ggtcggtaag cgctagacct
      481 caggccgctt ccagcgtcgg gtaatcggta tagccctcgg cgcccccgcc ataaaaggtc
      541 ttgctgttcg gtgcattgag cggcgcgccc tcgcgcaggc ggcgcaccag gtcggggttg
      601 ctgatgaagg gccggccgat cgccaccagg tcggcttcgc cgctttcgac cgcctgcatt
      661 gccatcgcgc ggtcatagcc gttgttgacc atccagggcg tgcgcaggcg ccggcgcagc
      721 gcctgatagt cgaagggggc gatgtcgcgc gcgccgccgg tctgaccctc gatcacatgc
      781 acgtacatca gctccagcgg cgcgagctgc tcggccgcgt agttgaacag cggctgcgga
      841 ttgctgtcgc gcgcgtcgtt ggccggcgtc accggcgaca ggcgcaggcc ggtgcgcgcc
      901 ctgccgatct cggtggtgac cgcgcgcatc acctcgagca ggaagcgtgc gcggttctcg
      961 atcgagccgc cgtaggcgtc ggtgcggtgg ttggagccgt cgcgcaggaa ctggtcgatc
     1021 agatagccgt tggcaccatg cacctcgacg ccgtcgaagc cggcctgcat cgcatggcgc
     1081 gcggcgtcgc gatactgggc aacgatggct ggaatctcgt gcagctccag ggcgcgcggc
     1141 tcagataccg gctcgaagcc gccgcggaca taggtcttgg tattggcgcg tatcgccgaa
     1201 ggcgccagcg gcgcggcgcc gccgggctgc agcgagacgt gcgaaacgcg ccccacatgc
     1261 cagagctgga tcacgatctt gccgccgcga gcatgcacgg cgtcggtgac cttcttccag
     1321 ccggcgatct gctcggcgct gtagatgccg ggcgtgtcga gatagccctg gccctcgggg
     1381 ctgatctggc tggcctcggt gacgatgagg ccggcaccat cgcgcggatg ggcgcgctgg
     1441 cggtagtact ccaccatcag ctcggtgggc acctggcccg cgccggcgcg gttgcgcgtc
     1501 agcggcgcca tcacgatgcg gttggcgagc gtgatgtcgc ccaggcgtat cgggtcgaag
     1561 agcttggtca tgcgggatcc ctcaatcacg gtcccgcaga tgctagaggt tgtgcgaagg
     1621 gcttgccgag gcaagccttt cgcgtttgtg cttgatcaaa tcgaaaccgt cgtcactagc
     1681 ccagcaccgt cgccagcagc cccgcgacgc ccaagcccag cgccgcgagc gcatcgcccg
     1741 cgattagccc gccgccgacc agcgaagtgg tgctcatgtc actgggcagg ccgccgccgt
     1801 catcgtcctt gcccggccgc ttcaggctgt tgagcgcgct ggacaggatg ccgcccgcag
     1861 cgaaccagga cgaggtggcc aggctcagga aggcgccgaa ggagaaggca tagggcgaag
     1921 gcagtatgaa ggcgtccatc acgaagtcgg cgctgaagcc gacgcgggcg ctgccttgca
     1981 cccagcgccg ccaggactcg ctggcgcgca gcagcttgcg cgccacttcg agcagcaggc
     2041 cgatcagcac gccgacccag atcgcggtgc gctggtaggg cttgtcgtcg gtcaggctgc
     2101 gcagcgcgcc gacgaatttg taggtcatcg ccgaggacca gttggccggc tgctggccgg
     2161 cgctcatcgt ggtctggtcc ag
//
LOCUS       DASUCY010000682         2458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1525679, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000682 DASUCY010000000
VERSION     DASUCY010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1525679"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VFV25_13470"
     CDS             complement(<1..235)
                     /locus_tag="VFV25_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEY1394377.1"
                     /translation="MPLLDVQHLAKRYGDTPVFENVSLQVQPGEFVALLGESGVGKST
                     LLNLIAALDRIDGGTVAIAGTEVQALAEAEQAHF"
     gene            complement(240..1364)
                     /gene="solA"
                     /locus_tag="VFV25_13475"
     CDS             complement(240..1364)
                     /gene="solA"
                     /locus_tag="VFV25_13475"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF008425.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HEY1394378.1"
                     /translation="MSPSFDVAVVGLGAMGSAAAYHLARQGRKVLGLDRFTSPHAFGS
                     SHGLTRIIREAYFEDPCYVPLVQRAYGLWDELQRLAGRELLRITGGLMIGPADGVLVG
                     GALRSAQEHGLAHELLSAAEVKRRFPALQPREDMVGVWEPRAGVLFPEACIAAHLALA
                     QAHKAALRGDERVLSWRAEADGAVRITTERSEYRARQLLLTAGAWIRSMVPELALPFS
                     VERQAVCWFAPTADPELFAPARCPVSLWETAPQKFLYGIPDFGDGMKLAVHHEGEEAA
                     PDGLRREVGEAEVAAIRALVQRFVPGANGPLRSSAVCMYTNTPDQHFWIDRHPQHPQV
                     LIASPCSGHGFKFAGVVGEALADWLITGQPRFDLSRFASR"
     gene            1504..1644
                     /locus_tag="VFV25_13480"
     CDS             1504..1644
                     /locus_tag="VFV25_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1394379.1"
                     /translation="MLPTEKDLLAEAERTVVRRAIWSLPRWVIWVALAACLAVSYFYG
                     GK"
     gene            1666..>2458
                     /locus_tag="VFV25_13485"
     CDS             1666..>2458
                     /locus_tag="VFV25_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEY1394380.1"
                     /translation="MKQVWITARGGPEVLRLQEAPDPQAQTGELRIRVKASGINFADI
                     LARKGLYPDAPKLPAVVGYEVSGFVDAVGPQVDSAWIGKEVFALTRFRGYADTVVVPQ
                     IQTFEKPAALSFVQAAAIPVNYLTAWQLLVVMGALKPDETVLIHNAGSGVGLAAIDIA
                     RHVGATIYGTASAGKHAFLKQRGLHEAIDYTRLDWTRELARLTQGRGVELITDPLGGA
                     EWKKSYRALRHTGRLGMFGASTATQSGLPGSLKLLSVGLGMPWFNP"
BASE COUNT          407 a          838 c          841 g          372 t
ORIGIN      
        1 ggaagtgtgc ctgctccgct tcggccagcg cttgcacctc ggtgcccgct atggcgaccg
       61 tgccgccgtc gatgcggtct agcgccgcga tgaggttgag cagcgtcgac ttgccgacgc
      121 ccgattcacc gagcagcgcg acgaactcgc cgggctgcac ctgcagcgag acgttctcga
      181 acacgggggt atcgccgtag cgcttggcaa ggtgctggac gtcgagcagg ggcatgagat
      241 cagcggctgg cgaagcggct caagtcgaag cgcggctgcc ccgtgatcag ccaatccgcc
      301 agcgcttcgc cgaccacgcc cgcgaacttg aagccgtgcc ctgagcaggg gctggcgatc
      361 agcacctgag ggtgctgcgg atgccggtcg atccagaaat gctggtccgg cgtgttcgtg
      421 tacatgcaca ccgccgacga gcgcagcggg ccgttcgcgc ctgggacgaa gcgctgcacc
      481 agggcgcgta tcgccgccac ctcggcctcg ccgacttcgc gccgcaggcc atcgggggcg
      541 gcctcctcgc cctcatggtg caccgcgagc ttcatgccat cgccgaaatc cggaatgccg
      601 tacaagaact tttgcggcgc ggtctcccag agactgaccg ggcatcgcgc cggcgcaaag
      661 agctcggggt ccgcggtcgg cgcgaaccag cacacggctt gccgctcgac cgagaacggc
      721 agcgcgagct ccggcaccat cgagcggatc catgcgcccg ctgtcaacag caattgcctg
      781 gcgcggtact cggagcgctc ggtggtgatg cgcacggcgc catcggcttc ggcccgccag
      841 ctcaggacgc gctcgtcgcc acgcagcgcg gctttgtgcg cctgcgccag cgcgagatgg
      901 gcggcgatgc aggcctcagg gaacagaacg ccggcgcgcg gctcccacac gccgaccata
      961 tcctcgcgcg gctgcagcgc ggggaagcgc cgcttcactt cggccgcgct cagcagctcg
     1021 tgagccaggc cgtgctcttg cgcgctgcgc agcgcgccgc cgaccagcac gccgtcggcg
     1081 gggccgatca tcagcccgcc ggtgatgcgc agcagctcgc ggccagcgag cctttgcagc
     1141 tcatcccaca agccataggc ccgctggact agcgggacat agcacgggtc ctcgaaatag
     1201 gcctcgcgaa tgatccgcgt cagcccgtgc gaggagccga aggcatgcgg cgatgtgaag
     1261 cgatccagcc ccagcacctt gcggccctgc cgagcgaggt ggtaggcagc ggcgctgccc
     1321 atcgcgccca ggccgaccac ggcaacatcg aaggatgggg acatcgggct ctttcctgaa
     1381 ctgtcggcgc gtaccgaccc gcgccccgga ggcggatgct acgctttcga tgcggcccac
     1441 gcgcacgcac aatgcctgca ggcccacaat gggccggcta catcaacatt cattgaggat
     1501 tgcatgctcc cgaccgagaa ggatttgctt gccgaagccg aacgcacagt cgtgcgccgc
     1561 gcgatatggt cattgccgcg ctgggtgatc tgggtggcac tggccgcctg cctggccgtg
     1621 tcctacttct acggtgggaa gtaagccgag cgtggagagc acgccatgaa gcaagtctgg
     1681 atcaccgcca ggggaggccc ggaagtcctg cggctgcagg aagcccccga cccgcaagcg
     1741 cagaccgggg agttgcgcat cagggtcaag gccagcggca ttaacttcgc ggacatcctg
     1801 gcccgcaagg gcctctatcc ggatgcgcct aagctcccgg cggtggtcgg ctacgaagtc
     1861 tcgggttttg tcgatgccgt cggcccgcag gtggatagcg cctggatcgg caaggaggtc
     1921 ttcgccctca cgcgctttcg cggctatgcc gacaccgtcg tggtgcccca gatccagacc
     1981 ttcgagaagc ctgccgcctt gagcttcgtg caggcggcgg cgatcccggt caactacctg
     2041 acggcctggc agttgctggt ggtgatgggc gcgctgaagc cggacgaaac ggtgctgatc
     2101 cacaacgcgg gcagcggcgt cgggctggcg gcgatcgaca tcgcccgcca tgtcggcgcc
     2161 acgatctacg gcaccgcgag cgccggcaag catgcctttc tcaagcagcg cggcctgcac
     2221 gaggcgatcg actacacccg gctcgattgg acgcgcgagc tggccaggct gacgcagggc
     2281 cgcggcgtcg agctcatcac cgacccgctg ggcggcgcgg aatggaagaa gagctatcgc
     2341 gccttgcgcc acacgggcag gctggggatg ttcggcgctt ccacggcgac ccaatcgggg
     2401 ctgcccggct cgctgaagct gctctcggtg ggcttgggca tgccgtggtt caacccgg
//
LOCUS       DASUCY010000683         2319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1011766, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000683 DASUCY010000000
VERSION     DASUCY010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011766"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="VFV25_13490"
     CDS             complement(<1..685)
                     /locus_tag="VFV25_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEY1394381.1"
                     /translation="MTRIDQLLVQQGLAPTRSAAQRLIERGAVRWLGPKGWTVPRKAG
                     DELPEGCRIEITDDAELRYVSRGGLKLEGALVHTGIAVQGSICLDLGQSTGGFTDVLL
                     TRGARQVIGVDVGHGQLAPQLREDARVTALEGINARELSAAMLKAAGVAQPRFDLIVG
                     DLSFISLTLVLPALAPLLAQGGELLLLVKPQFELPGCGPEHRRLHRCASGARCEAGGR
                     HRRGPWPARA"
     gene            complement(740..2173)
                     /gene="ahcY"
                     /locus_tag="VFV25_13495"
     CDS             complement(740..2173)
                     /gene="ahcY"
                     /locus_tag="VFV25_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEY1394382.1"
                     /translation="MNATLKPAAAADFKVADLSLAAWGRKEIAIAETEMPGLMAIREE
                     FAATQPLRGARITGSLHMTIQTAVLVETLQALGATVRWASCNIFSTQDHAAAALVAAG
                     TPVFAYKGESLADYWDYTHRIFEFGAKGAEGEGPNMILDDGGDATLLMHLGRRAEKDL
                     SVLAKPGSEEERELFAAIKAKLAEDANWYTRKSAAIVGVTEETTTGVHRLKEMSAKGT
                     LLFRAINVNDSVTKSKFDNLYGCRESLVDGIKRATDVMIAGKIAVVCGYGDVGKGSAQ
                     ALRALSAQVWVTEIDPINALQAAMEGYRVVTMDYAADKADIFVTATGNKHVITHDHML
                     AMKDQAIVCNIGHFDNEIDIASVEKYQWEEIKPQVDHVIFPTGRRIILLAKGRLVNLG
                     CATGHPSYVMSSSFANQTLAQIELYTKPQAYEVGQVYVLPKHLDEKVARLQLSKLGVQ
                     LTELSDEQAAYIGVDKQGPYKPDTYRY"
     regulatory      complement(2190..2283)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
BASE COUNT          338 a          782 c          791 g          408 t
ORIGIN      
        1 cggcgcgagc tggccatggc ccacgtcgat gccggccacc tgcctcgcac cgcgcgccag
       61 aagcacatcg gtgaagccgc cggtgctctg gcccacatcc aggcagctcg aactgcggct
      121 tgacgagcag cagcagctct ccgccctgcg cgagcagcgg cgccagcgcc ggcagcacca
      181 gcgtgagcga gatgaaggac aggtcgccga cgatgaggtc gaagcgcggc tgcgcgacgc
      241 ccgccgcctt cagcatcgca gcggacagct cgcgcgcatt gatgccttcg agcgccgtca
      301 cgcgcgcgtc ttcgcgcagc tgcggcgcga gctggccatg gcccacgtcg acgccgatca
      361 cctgcctcgc gccgcgcgtg agcagcacat cggtgaagcc gccggtgctc tggcccaggt
      421 ccaggcagat cgagccctgc acggcgatgc cggtgtgcac cagcgcccct tcgagcttga
      481 ggccgccacg cgagacatag cgcagctcgg catcgtcggt gatctctatc ctgcagccct
      541 cgggcagctc gtcgccggcc ttgcgcggca ccgtccagcc cttggggccg agccagcgca
      601 ccgcgccgcg ctcgatcagc cgctgcgcgg ccgagcgcgt gggcgcgagc ccttgctgca
      661 cgagtagctg gtcgatgcgc gtcattgctg cacgccgcgt ccgtggccgc tcgaagggag
      721 cggccacagg ccgtggtcgt cagtatctgt aggtgtcggg cttgtagggg ccttgcttgt
      781 ccaccccgat ataggccgcc tgttcgtcgc tcagctcggt gagctgcacg ccgagcttgg
      841 acagctgcag ccgcgcgacc ttctcgtcca ggtgcttggg cagcacatag acctggccga
      901 cctcataagc ctgcggcttg gtgtagagct cgatctgcgc cagcgtctgg ttcgcgaagc
      961 tggacgacat cacatagctc gggtggccag tggcgcagcc cagattcacg aggcggccct
     1021 tggcgagcag gatgatgcga cgtcccgtgg gaaagatcac gtggtcgacc tgcggcttga
     1081 tctcttccca ctggtacttc tcgaccgagg cgatgtcgat ctcgttgtca aagtggccaa
     1141 tgttgcagac gatggcctga tccttcatcg ccagcatgtg atcgtgcgtg atcacatgct
     1201 tgttgccggt ggcggtgacg aagatgtcgg ccttgtcggc cgcgtagtcc atggtcacga
     1261 cgcgatagcc ttccatcgcg gcctgcagcg cgttgatggg gtcgatctcg gtcacccaga
     1321 cctgggccga cagcgcgcgc agcgcctgcg ccgagccctt gcccacgtcg ccatagccgc
     1381 agaccacggc gatcttgccg gcgatcatca cgtcggtggc gcgcttgatg ccgtccacca
     1441 gcgactcgcg gcagccgtag aggttgtcga acttgctctt ggtgacgctg tcgttgacgt
     1501 tgatggcgcg gaacagcagc gtgcccttcg cgctcatctc cttcaggcgg tgcacgccgg
     1561 tggtggtctc ttcggtcacg ccgacgatgg ccgcgctctt gcgcgtgtac cagttcgcgt
     1621 cctcggccag cttggccttg atggcggcga agagctcgcg ctcctcctcg ctgccgggct
     1681 tggcgagcac cgagaggtcc ttctcagccc tgcggccgag atgcatcaag agcgtcgcat
     1741 cgccgccgtc gtcgaggatc atgttggggc cttcgccctc ggcacccttg gcgccgaact
     1801 cgaagatgcg gtgcgtgtag tcccagtagt cggcgagcga ctcgcccttg tacgcgaaca
     1861 ccggcgtgcc ggcggccacg agcgcggcgg cggcgtggtc ctgggtggag aagatgttgc
     1921 acgaggccca gcgcacggtg gcgccgagcg cctgcagcgt ctcgaccagc acggcggtct
     1981 ggatggtcat gtgcagcgag ccggtgatgc gcgccccgcg cagcggctgg gtcgcggcga
     2041 attcttcgcg gatcgccatg aggcccggca tctcggtctc ggcgatggcg atctccttgc
     2101 gcccccaggc ggcgagtgac aggtccgcga ccttgaaatc agcggcggcg gcaggtttga
     2161 gtgtggcgtt catacagtac tccgtgcaga cagagcgagc gccgtatggt gttccgggtc
     2221 ccgctgagcc tggcaggtgg cccgggggat cccggccgtc ctgtcgcaac gctcctcagc
     2281 gggcgcggct gccgcatcaa gcggcggaaa gcctcattc
//
LOCUS       DASUCY010000684         2746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1301025, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000684 DASUCY010000000
VERSION     DASUCY010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301025"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..528)
                     /locus_tag="VFV25_13500"
     CDS             complement(76..528)
                     /locus_tag="VFV25_13500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEY1394383.1"
                     /translation="MRPRERALLILIDGQMSDDVAAGAARTLGLELDAIDELARRGLI
                     ERTAPGRAPAATAKAASADHARAPDEQRIWAAAKLQMLGLLDLALGGRSSPLRDASRL
                     AVDEMSLLAWIEHCLGQVETMAGMEASDKIRQRMLDVLPAGAIVALAV"
     gene            765..1187
                     /locus_tag="VFV25_13505"
     CDS             765..1187
                     /locus_tag="VFV25_13505"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEY1394384.1"
                     /translation="MSDSSDDVLARIRAVNDMAAFNRWCGIEVVAAEHGAATIAMPWR
                     GEAAQYSGFLHAGLLGALIDTACGFAAATLVGRVLASHYAVNCLRPAVGSRFIARARV
                     VKPGKSQIFTSCELYAESQDGEKLVATGETLLVVVGEG"
     gene            1380..2636
                     /gene="egtB"
                     /locus_tag="VFV25_13510"
     CDS             1380..2636
                     /gene="egtB"
                     /locus_tag="VFV25_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HEY1394385.1"
                     /translation="MRRYEAVRTLSLRLAAPLSAEDQCVQSMPDASPIKWHLAHTSWF
                     FETLVLMPHARGYRAHDERYQFLFNSYYESLGPRHPRPQRGLLTRPSADEVLYYREKV
                     DEAVAHFIEAAPAAQWAGVAPLLMLGLQHEQQHQELMLADIKHLLSLNPLQPAYIERP
                     AHAVAAGASKAVLTEWLQFGGGVVEIGHDGAGFAFDNETPRHDVLLRPYELASRPVSC
                     GEYLAFVRDGGYRRPELWLSDGWAAVQAQGWTAPLYWSEVDADQPTVFTLFGPQPLQA
                     GEPVCHLSLFEAAAYAAWACARLPTEFEWEAAAERCAADESPRYEPEHPHPRAADEET
                     QPGLQQMLGEVWEWTRSSYDPYPGFKPLAGAVGEYNGKFMVGQNVLRGGSCASPPGHL
                     RASYRNFFPPAARWQFSGLRLARDAA"
BASE COUNT          417 a          905 c          972 g          452 t
ORIGIN      
        1 tcctgtggcg gcactcagtc ggcgtcggcg caagggagat gggcacctcg ggggatgtgc
       61 gggagcgccg ccaggtcaga cggccaaggc cacgatagct ccagccggca ggacgtcgag
      121 catgcgttgg cggatcttgt ccgatgcctc catgccagcc atcgtttcca cctggcccag
      181 gcaatgctcg atccaggcga gcagggacat ctcgtcgacc gctaggcgcg aggcgtcccg
      241 caggggggat gagcgtcccc ccaatgcgag atccagcaag ccgagcatct gaagcttggc
      301 ggcggcccat atgcgttgct cgtctggagc gcgcgcgtgg tcagccgagg cggccttggc
      361 ggtagcggct ggagcgcggc ccggcgcggt ccgttcgatg agtccgcgcc gtgccagctc
      421 gtctatggca tcgagctcca ggcccagggt tctcgcggcg cccgccgcca cgtcatcgga
      481 catctgccca tcgatcagga tgagcagcgc ccgttcgcgc gggcgcaagc ctgccgagcg
      541 aacggccagg gccgatcgcc cggcatcggt ctttttccag gcgggcattg cagatcaatc
      601 gcggattctt tgggaggcgg cagcttagtg cctgcgggtg acaaatctgc gacgggatgg
      661 ctgtgccggc accggcgctg gcgctcgatc gtgtcgccgc tttgacagcg ggtgccactc
      721 tcggggccta gattgatgcc atcttttcga agaggagccg gcccatgagc gattcatccg
      781 atgatgtcct ggctcgcatc cgggcggtca atgacatggc tgctttcaac cgctggtgcg
      841 gcatcgaggt cgtcgcggca gagcatggag cggccaccat cgccatgccg tggcgtggcg
      901 aggccgcgca gtactccggc tttctccacg caggccttct cggcgcgctg atcgacactg
      961 cctgcggctt tgccgcggcg acgcttgtgg gccgcgtcct ggcttcgcac tacgccgtca
     1021 actgcctgcg gcctgccgta ggcagtcgct tcatcgcccg ggcgagggtc gtcaagcccg
     1081 gcaagtcgca gatcttcacg tcatgcgagc tatacgccga gagccaagac ggcgagaagc
     1141 tcgtggccac gggggaaacg ctgctggtgg tcgtcggcga gggctgaccg tctgccgcag
     1201 gcgactgccg cgggcttggc cgcacagctt cggtgagccg gcaaccccgc ccctgcgcag
     1261 aaggcgcacc agtgctaccc tcccgcttgc tgcctcatcc aggcaggcag gaggatgcgt
     1321 tgagcagcgt cagcatcgat caccaggctc tcgccgccct cgagccgcgc agcgcctggc
     1381 tgcgccgcta cgaggcggtg cgcacgctgt cgctgcggct ggcggcgccg ctctcggcgg
     1441 aggatcaatg cgtgcagtcg atgccggatg cgagcccgat caagtggcat ctggcgcaca
     1501 cgagctggtt cttcgagacg ctggtgctga tgccgcatgc gcgcggctac cgggcgcatg
     1561 acgagcgcta tcaattcctc ttcaattcct attacgaatc gctgggcccg cggcatccgc
     1621 ggccgcaacg cggcttgctg acgcggcctt cggcggacga ggtgctgtac taccgcgaga
     1681 aggtggacga ggcggtggcg catttcatcg aagcagcgcc tgcggcccag tgggccgggg
     1741 tcgcgccgct gctgatgctg ggcctgcagc acgagcagca gcaccaggag ctgatgctgg
     1801 ccgacatcaa gcatctgctg tcgctcaacc cgctgcagcc ggcatatatc gagaggccgg
     1861 cgcatgccgt tgcggcgggc gcatcgaaag cggtgctgac cgagtggctg caattcggcg
     1921 gcggcgtggt cgagatcggc cacgatggcg caggcttcgc cttcgacaac gagacgccgc
     1981 gccacgatgt gctgctgcgg ccctacgaac tggcgagccg gccggtgagc tgcggggaat
     2041 acctggcctt cgtgcgcgac ggcggctatc gccgccccga gctctggctc agcgacggct
     2101 gggccgcggt gcaggcccag ggctggaccg cgcccctgta ttggagcgag gtggacgccg
     2161 accagccgac cgtgttcacg ttgttcgggc cgcagccctt gcaagcgggc gagcctgtct
     2221 gccacctgag cctgttcgag gcggcagcct atgcggcttg ggcgtgcgcg cgcttgccca
     2281 ccgagttcga gtgggaagcg gcggccgagc ggtgtgccgc cgatgaaagc ccgcgctacg
     2341 aacccgagca cccgcatccg cgtgcggccg acgaagagac gcagccgggc ctgcagcaga
     2401 tgctgggcga ggtatgggag tggacgcgct cgtcctacga cccctatccc ggcttcaagc
     2461 ccctggcagg tgcggtgggc gagtacaacg gcaagttcat ggtgggccag aatgtgctgc
     2521 gcggcggtag ctgcgcctcg ccgcctggcc atctgcgcgc gagctatcgc aatttctttc
     2581 cgccggctgc acgctggcag ttctccggtt tgcgcctggc aagggatgcc gcatgaagaa
     2641 cccgaatctg ctgcgggcca cgcccgatct gcgcgcgctg ccgcagcggc agacgagcgc
     2701 gcgcgacgaa gccgcgggca gcgacttcgc acaggcgatg ctgcag
//
LOCUS       DASUCY010000685         4636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_642468, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000685 DASUCY010000000
VERSION     DASUCY010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642468"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="VFV25_13515"
     CDS             <1..925
                     /locus_tag="VFV25_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEY1394386.1"
                     /translation="PAFDGQGAAAFTAAGMLSPLAEQECGGAEVAQLGWASLALWPRI
                     VERLQGLQPRLRIAGSLLLAHGADRGAAARALTRMPGAQALDAQALCELEPALAPGLL
                     AWLLPGEGLIHPIEAMAALHADAGGAQWHWGRRVERVLPGRLLLDGGEVASCDWAIDA
                     RGLGARPALPLRGVRGEVVALALRAHGLQRPVRLLHPRHRVYLVPRSEDELLLGASEI
                     ESEDRSPVSLQSAVELMAAAHSVMPALAEARIARLDRNLRPALPDNLPHTHAEPGLLR
                     LNGLYRHGWLLAPALVQQLLAEAGLASLESE"
     gene            922..1131
                     /gene="thiS"
                     /locus_tag="VFV25_13520"
     CDS             922..1131
                     /gene="thiS"
                     /locus_tag="VFV25_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEY1394387.1"
                     /translation="MKTITVNLDERPLELPAGSTLADLLRHEQLAPDGAATACNGHFV
                     PRECRAGTVLQDGDQVLLFKPIVGG"
     gene            1137..1946
                     /locus_tag="VFV25_13525"
     CDS             1137..1946
                     /locus_tag="VFV25_13525"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEY1394388.1"
                     /translation="MNTTNTNDDALLIAGTALNSRFLLGSAGYPSPQVLTESIRASET
                     QVLTVGLKRTLQAGDNGFVAQALACAQAQGARLLPNTAGCRSAREAVALASMARELYA
                     TSWIKLEVVGDEHTLQPDPFELLAAAAELVKLGFTVFPYCTDDLVLCRRLLDAGCAVL
                     MPWGAPIGSGQGLLHLHALRTLRERLPEAVLIIDAGLGAPSHAAQALELGFDAVLLNS
                     AVAQAREPVAMAAAFRDAVRAGRRAHLAGLMAPQDFAVASTPVSGHAFLLG"
     gene            1949..3637
                     /locus_tag="VFV25_13530"
     CDS             1949..3637
                     /locus_tag="VFV25_13530"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:NF020132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEY1394389.1"
                     /translation="MDDETLRFPHPCLPSPFAEPVDPDAARLPSWLQDPRPPVIWSVA
                     GTDSGGGAGHAADQRAAAAMGVHLCPVVAAVTAQNSQGVQAVYPLPAEQIEAQMLALS
                     QDLRPRVVKTGLLGSTGAVEAVARSIDVLRERGPVGLVVDPVLGATAGGAAFADAAVL
                     AAYRELLLPRATLVTPNRREAERLAGVAPGAGRIPELAAELMRLGPQAVCITGGDEQV
                     EQGSDELALDWFTGHIGPWLPNLGSTSGWLALPRLASRHHHGSGCSFASGAAAALARG
                     FALPDAMVLAKMLAWCGIRDGHAAGAGSGPLRPSAGLVCDPRAMPVMSFGDEPLDEVI
                     LARWRRVLSAEHGVREHAGFARGLYAITDQPQRVEALAASGCFAHVQLRIKSRSGADE
                     AQEQGRIRAAVRQAVGGVGDSACRLWINDHWRLALEEGAPALHLGQEDWTALSRDDKA
                     LLLAEREAGRLELGLSSHSLWELARARGLAPSYVACGPVWETTTKRMPWLPQGLSHLA
                     WWTRMAGRPVVAIGGILSSQRLARCAQAGASGACLVRAAEDSARWAEFAQAWRR"
     gene            complement(3648..4478)
                     /locus_tag="VFV25_13535"
     CDS             complement(3648..4478)
                     /locus_tag="VFV25_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEY1394390.1"
                     /translation="MHDTAARRPAAGDYLRAWRQRRHMSQLEFALEAEVSQRHLSFLE
                     TGRSVPSREMLIRLAERLEVPLRDRNAMLIAAGYAPIYAERPLADPAMAAARQAIELV
                     LKGHEPYPALAIDRHWHLQAANAALGPLLAGVKDKSLLEPPVNVLRLSLHPQGLASRI
                     ANLAEWRCHLLDRLRRQIAVTADPVLVDLLKELNAYPASAGDAVAQDAPSDHGGVLVP
                     LELVTDAGLLSFISTTTVFGTPRDITLSELAVEAFFPTNDATAEILRRLGTTEAAAAA
                     "
     gene            complement(4528..>4636)
                     /locus_tag="VFV25_13540"
     CDS             complement(4528..>4636)
                     /locus_tag="VFV25_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY1394391.1"
                     /translation="PEGEPDLIKGTDFVLLDGGLLKAVTGFLDKVPAGA"
BASE COUNT          615 a         1617 c         1707 g          697 t
ORIGIN      
        1 gccggccttc gatggccagg gcgctgcagc cttcacggcc gcgggcatgc tgagcccgct
       61 ggccgagcag gaatgcggcg gcgcggaggt cgcgcagctc ggctgggcgt cgttggcgct
      121 gtggccgcgc atcgtcgagc gcctgcaggg cctgcagccg cgcctgcgca tcgccggcag
      181 cctgctgctg gcccatggtg cggatcgcgg cgcggcggcc cgcgcgctga cgcgcatgcc
      241 gggcgcccag gccttggacg cgcaggccct gtgcgagctg gaacccgcgc tcgcgcccgg
      301 cctgctggcc tggctgctgc cgggcgaggg cttgatccac ccgatcgaag ccatggccgc
      361 gctgcatgca gacgccgggg gcgcgcaatg gcactggggg cggcgcgtcg agcgggtgct
      421 gcccggccgg ctgctgctcg acggcggtga ggtcgcatcg tgcgactggg ccatcgatgc
      481 gcgcggcctc ggcgcgcggc ctgcgctgcc gctgcgcggc gtgcgcggcg aggtggtcgc
      541 tctcgcgctg cgcgcgcatg gcctgcagcg gccggtgcgc ctgctgcatc cgcgtcatcg
      601 cgtctacctg gtgccgcgca gcgaggacga actgctgctg ggggccagcg agatcgagag
      661 cgaggaccgc tcgccagtca gcctgcaatc ggccgtcgag ctgatggccg cggcgcacag
      721 cgtcatgccc gcgctggccg aggcccgcat cgcgcggctg gaccgcaacc ttcggcccgc
      781 cttgccggac aacctgcccc atacccatgc cgagcctggg ctgctgcggc tcaacggctt
      841 gtaccgccac ggctggctgc tggcccccgc cttggtgcag cagttgctcg ccgaggccgg
      901 gctagcaagc ctggaatcgg aatgaagacc atcactgtca accttgacga gcgcccgctg
      961 gagctgccgg ccggcagcac gctggccgat ctgctccgcc acgagcagct cgcaccggac
     1021 ggcgccgcta ccgcctgcaa cggccacttc gtgccgcgcg aatgccgggc tggcaccgtg
     1081 ctgcaggacg gcgaccaggt cctgctgttc aagcccatcg taggaggctg aatcccatga
     1141 acaccacgaa caccaacgac gacgccttgc tcatcgccgg cacggcgctg aacagccgct
     1201 tcttgctcgg cagcgccggc tatcccagcc cgcaggtgct gacggagtcg atacgcgcct
     1261 ccgaaaccca ggtgctcacc gttggcctca agcgcacgtt gcaggcgggc gacaacggct
     1321 tcgtggcgca ggcactggcc tgtgcccagg cacaaggggc gcgcctgctg cccaacaccg
     1381 ccggctgccg cagcgcgcgc gaggcggtgg ccctggccag catggcgcgc gagctctacg
     1441 cgacgtcatg gatcaagctc gaggtggtgg gcgacgagca cacgctgcag cccgatccct
     1501 tcgagctgct ggcggccgcg gccgaactgg tcaagctcgg attcacggtc tttccctatt
     1561 gcaccgacga cctggtgctg tgccggcgcc tgctcgacgc cggctgcgcc gtgctgatgc
     1621 cctggggcgc gcctatcggc tcgggccagg gcctgctgca cctgcacgcc ctgcgcacgc
     1681 tgcgcgagcg cctgcctgag gccgtgttga tcatcgacgc gggcctcggc gcaccttcgc
     1741 acgcagcgca ggcgctggag ctgggcttcg atgcggtgct gctcaattcc gccgtggcgc
     1801 aggcccgcga gccggtggcg atggccgcgg ccttccgcga tgcggtgcgc gccgggcggc
     1861 gggcgcatct agcggggctg atggcgccgc aggacttcgc ggtcgcgagc acgccggtca
     1921 gcggtcatgc ctttctgctg gggtgagcat ggacgacgag acgctgcgct tccctcatcc
     1981 ctgcctgccg tcgccctttg ccgagcccgt cgacccggac gctgcgcggc taccctcctg
     2041 gctgcaagac ccaaggccac cggtgatctg gagcgtggcc ggcacggaca gcggcggcgg
     2101 cgccggtcat gcggcagacc agcgtgctgc cgcggccatg ggcgtgcatc tgtgccccgt
     2161 ggttgccgcc gtgacggcgc aaaactccca gggcgtgcag gccgtctacc cgctgccggc
     2221 ggagcagata gaggcccaga tgctcgcgct cagccaggac ctgcggccac gtgtcgtcaa
     2281 gaccggcctg ctgggcagca caggcgccgt cgaggcggtg gcgcgcagca tcgacgtcct
     2341 gcgcgagcgg ggcccggtcg gcctcgtcgt cgatccggta ttgggcgcca cggcaggcgg
     2401 cgcggcgttt gcggacgcgg ccgtgctggc cgcctatcgc gagctgctgc tgccgcgcgc
     2461 cacgctcgtc acgcccaacc ggcgcgaggc ggagcgcctg gccggcgtgg cgcccggcgc
     2521 ggggcgcata ccggagctgg ctgccgagtt gatgcggctc gggccgcaag ccgtttgcat
     2581 caccggcggt gacgagcagg ttgagcaggg atccgatgag ctggctctcg actggttcac
     2641 cggccacatc ggcccctggc tgccgaatct gggcagcacc tcgggctggc tcgcgctacc
     2701 gcgcctcgcc tcacgccatc atcatggcag cggctgcagc ttcgccagcg gcgcggcggc
     2761 ggcgctggcg cggggcttcg cgctgcccga tgcgatggtg ctggccaaga tgctcgcgtg
     2821 gtgcggcata cgcgacggcc atgctgcggg cgccggctca gggcccttgc gtccgagtgc
     2881 cggcctcgtc tgcgatccgc gcgccatgcc ggtgatgagc ttcggcgacg agccgctgga
     2941 cgaggtgatc ctggcgcgat ggcgccgcgt gctgagcgca gagcatggcg tgcgggaaca
     3001 tgccggcttt gcgcgtggcc tgtacgcgat cacggatcag ccgcaacgcg tcgaggcgct
     3061 ggccgcttcg ggctgctttg cgcacgtcca gctgcgcatc aagtcgcgat ccggtgccga
     3121 cgaagcgcag gagcaagggc gcatccgcgc cgccgttcgg caggctgtcg gcggagttgg
     3181 cgatagcgcc tgccggttgt ggatcaatga ccattggcga ctggcgctgg aagagggcgc
     3241 cccggccctg cacctcggcc aggaggattg gaccgcgctg agccgcgacg acaaggcgct
     3301 gctgctggcc gaacgcgagg ccgggcgcct cgagctgggc ctgtccagcc acagcctctg
     3361 ggagctggcg cgtgcgcgcg gcctggcgcc cagctatgtc gcttgcgggc cagtctggga
     3421 gacgaccacc aagcgcatgc cctggctgcc gcaagggctc agtcatctgg cctggtggac
     3481 gcgcatggcg ggacgtccgg tggtcgccat cggcggcatc ctgtccagcc agcgactcgc
     3541 acgctgcgcg caggccgggg cctccggcgc ctgcctggtc agggccgcgg aggatagcgc
     3601 tcgctgggct gaattcgccc aggcctggcg gcgctgacga aaacagatca ggcggcggcc
     3661 gcggcttctg tggtgccgag ccgcctcagg atctccgcgg tagcgtcgtt ggtcggaaag
     3721 aaggcctcca ccgcgagttc cgacagcgtg atgtcgcgtg gcgtgccgaa cacggtggtg
     3781 gtcgagatga aggagagcag ccctgcatct gtcaccagct ccagcggcac cagcacgccg
     3841 ccatggtcgc tgggcgcgtc ttgagctacg gcatctccgg cgctggcagg gtaggcgttc
     3901 agctctttca atagatcgac cagcacggga tcggccgtga ctgcgatctg gcgccgcagg
     3961 cgatccagca ggtggcagcg ccattccgcg agattggcga tgcgtgaggc cagcccctgc
     4021 gggtgcaggc tgagccgcag cacgttcacc ggcggttcga gcaaggactt gtccttcacg
     4081 ccggcgagca gcggccccag cgcggcgttg gccgcctgca gatgccaatg ccggtcgata
     4141 gccagcgccg ggtagggctc atggcccttc agcaccagtt cgatggcttg ccgcgctgca
     4201 gccatcgcgg ggtcggccag cggccgctcg gcatagatcg gcgcatagcc ggccgcgatc
     4261 agcatggcat tgcggtcgcg cagcggcact tccagccgct cggccaggcg gatgagcatc
     4321 tcgcggctgg gcaccgagcg gccggtttcg agaaagctca ggtggcgctg ggagacctcg
     4381 gcctccagcg cgaactcgag ctggctcatg tgccgccgct ggcgccaggc tcgcaggtag
     4441 tcgcctgcgg cgggccttct ggcggcggtg tcatgcatgg cgacgcgctc tcctcatggg
     4501 gcttggatgg gggcaggcaa acggcgttca ggcgccggct gggaccttgt cgaggaagcc
     4561 ggtcacggcc ttgagcaggc cgccgtcgag caggacgaag tccgtgccct tgatcaagtc
     4621 cggctctccc tcgggc
//
LOCUS       DASUCY010000686         2292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_272663, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000686 DASUCY010000000
VERSION     DASUCY010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272663"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..424)
                     /locus_tag="VFV25_13545"
     CDS             complement(32..424)
                     /locus_tag="VFV25_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY1394392.1"
                     /translation="MKLGYTILYVADVEASIAFCERAFGLQRGFVHESGAYGELATGA
                     TTLAFAQHETARSNLGQNYVDAAASKQPLGMEIGLVTEDVPAAYRRAVQAGAAALCDP
                     QRKPWGQTVAYVRCPDGLLVELCTPMGD"
     gene            complement(528..905)
                     /locus_tag="VFV25_13550"
     CDS             complement(528..905)
                     /locus_tag="VFV25_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY1394393.1"
                     /translation="MQTRNALNWFEIPVRDLARASRFYEALLDKPLRRENMGANQLAV
                     FSYEQPGVGGCLIQGPEMPAPADNGTTVYLDASPSLDAVLARVETAGGRIATPRIDLP
                     GDIGCFAHVLDTEGNRVGLHALA"
     gene            complement(1013..>2292)
                     /locus_tag="VFV25_13555"
     CDS             complement(1013..>2292)
                     /locus_tag="VFV25_13555"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol kinase"
                     /protein_id="HEY1394394.1"
                     /translation="AAIGITNQRETTVLWNRRTGEPIHKAIVWQDRRTEPICAELRAR
                     GLAGTVQARTGLVIDPYFSGTKLKWLLDHVPGAREQAKRGELAFGTVDSWLLWRLTGG
                     RVHATDVSNASRTMLFDIHRNAWDAELLAALDIPAEVLPKVQPSSSDFGSSDAKWLDS
                     PIPIGGIAGDQQSALFGQACFRAGLAKNTYGTGCFMLMHLDGRFQTSANGLITTSAAQ
                     PAARPEYALEGSVFIGGAVVQWLRDGLHAIKGSAEVQALAESVPDAGGVIFVPAFTGL
                     GAPYWKPKASGAIVGLTRGSTVAHIARAALESIAFQSAALLQAMSRDAQAAGGAPVSE
                     LRVDGGASVNDLLMQFQADLLGIPVVRPRVIETTALGAAYLAGLSCGVYAGLDELAAQ
                     WQVERRFLPTLPRERAQELMTRWERAVRQATLG"
BASE COUNT          339 a          757 c          816 g          380 t
ORIGIN      
        1 ctgaaacagg cggtcggcac ggcgcacgcg ttcaatcccc catgggcgta cacagctcga
       61 ccagcagccc gtcggggcag cgtacatagg ccacggtctg gccccagggc ttgcgctgtg
      121 gatcgcacaa ggccgctgcg ccggcctgca ccgcgcgccg gtaggcggcg ggcacgtctt
      181 cggtgacaag gccgatctcc atgcccagcg gttgtttcga agcggccgca tccacgtagt
      241 tttgcccgag attgctgcgc gccgtttcgt gctgggcgaa ggccagcgtg gtggcaccgg
      301 tggcgagctc gccataggcg ccggactcgt gcacgaaacc gcgctgcagg ccgaaggcgc
      361 gctcgcagaa ggcgatggaa gcctccacgt cggcgacgta gaggatggtg tagccgagtt
      421 tcatcgtcgt gtgcatgatg ctcgtcctcc acagggatga acccgcatgc ggggcatgcg
      481 ggcattgtgg gccgactgcg cttttgctgc atgccggccc ccagagctca tgccaaggcg
      541 tgcaggccga cgcgattgcc ttccgtgtcc agcacgtgag cgaagcagcc tatgtcgccc
      601 ggcaggtcga tgcgcggcgt cgcgatgcgc ccgccggccg tctcgacgcg ggctagtact
      661 gcatccagcg agggtgaggc gtcgaggtag acggtggtcc cgttgtcggc cggcgcgggc
      721 atttccggcc cctggattag gcagccgccg acgccgggct gctcgtagga aaagacggcc
      781 aactgattgg cccccatgtt ttcccggcgc agcggcttgt cgagcagggc ctcgtagaag
      841 cggctggcgc gggccaggtc gcgcacgggg atttcgaacc agttgagggc attgcgggtt
      901 tgcatgttgg gctccttcat caagaggtgg tgattgggag ccgtgatggt gcgtgggggc
      961 tcctgacagc gtggtggcag gagggtgggt cggggtgccg ggagttcgcc ctctacccca
     1021 gcgtcgcctg cctcaccgca cgctcccagc gcgtcattag ctcctgcgcc cgctcgcgcg
     1081 gcagggtcgg caggaagcgc cgttccacct gccactgtgc tgccagctcg tcgaggcccg
     1141 catagacgcc gcagctcagg ccggcgagat aggcagcgcc caaggccgtc gtttcgatca
     1201 cgcgcggccg caccaccgga atgcccagca gatcagcctg gaactgcatc aacaggtcgt
     1261 tgacgctggc gccgccgtcg acgcgcagct cgctcaccgg cgcgccgccg gcagcctgcg
     1321 cgtcgcggct catcgcctgc agcaatgcgg cgctctggaa ggcgatgctc tccagcgccg
     1381 cacgcgcaat atgcgccacg gtgctgccgc gcgtgaggcc gacgatggcg ccgctggcct
     1441 tgggcttcca ataaggagcg ccgaggcccg tgaaggcggg cacgaagatc acgccgcctg
     1501 cgtcgggcac gctttcggcc agcgcctgca cctcggccga gcccttgatt gcatgcaggc
     1561 cgtcgcgcag ccattgcacg acggcgccgc ctatgaagac actgccttcg agcgcgtact
     1621 ccggccgcgc cgccggctgg gcggcgctgg tcgtgatgag gccgttggcg ctggtctgga
     1681 agcgcccgtc caggtgcatc agcatgaagc agccggtgcc ataggtgttc ttcgccaggc
     1741 cggcccggaa gcaggcctgg ccgaacagcg cgctctgctg gtcgccggcg atgccgccaa
     1801 tggggatggg gctgtccaac catttcgcat cgctgctgcc gaagtccgag ctggagggct
     1861 gcactttggg cagcacctcg gccgggatgt ccagcgcggc aagcagctcg gcgtcccagg
     1921 cattgcggtg gatgtcgaac agcatcgtgc gcgaggcatt gctgacatcg gtggcgtgga
     1981 ccctgccgcc cgtgagccgc cacaacagcc agctgtcgac cgtgccgaag gccagctcgc
     2041 cgcgctttgc ttgctcgcgc gcgcccggca catggtcgag cagccatttg agcttggtgc
     2101 cggagaaata ggggtcgatc acgaggccgg tcctggcctg gacggtgccg gcgaggccgc
     2161 gcgcgcgcag ctcggcgcag atcggctcgg tgcgccggtc ctgccagacg atggccttgt
     2221 gtatcggctc gccggtcctg cggttccaca agaccgtcgt ttcgcgctgg ttggtgatgc
     2281 cgatcgcggc aa
//
LOCUS       DASUCY010000687         2867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1397298, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000687 DASUCY010000000
VERSION     DASUCY010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397298"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="VFV25_13560"
     CDS             <1..909
                     /locus_tag="VFV25_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEY1394395.1"
                     /translation="ELGMNHPGEIALLARWAAPTIALVNNAQREHQEFMATVEAVARE
                     NGTAIQALSANGIAVFPADDAYTPVWRELAAQRKTLSFALDGLGGPADVSGSAAWAGD
                     HWRLEIRTPAGTTNLKLRVAGAHNLKNALAAAACALAAGCPLEAIARGLEAFEPVKGR
                     SQLQRLKLAGRELTLIDDSYNANPDSMRAAIEVLASLPAPRWLVMGDMGEVGEQGPAF
                     HREVGSYAAQRGIEHLWAAGGQSAAAAAAYREAGGADVRHFESAAAVVAALAQAPAAA
                     SAVIKGSRFMKMEQVVAALKGAQDAR"
     gene            930..2111
                     /gene="mraY"
                     /locus_tag="VFV25_13565"
     CDS             930..2111
                     /gene="mraY"
                     /locus_tag="VFV25_13565"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEY1394396.1"
                     /translation="MLVSLAQWLQSAYPEQLGFLRIFQYLTFRAVMAAMTALLIGLTF
                     GPFVIRRLAELKIGQPIREYGVQSHMVKRGTPTMGGVLILLAIAASTLLWFDWSNRFV
                     WIVMLVTTGFGAIGWVDDWRKVVHKNPEGMRSREKYFWQSVIGLVAALYLAFSVSEIS
                     NLRVLELFLRWVQSGFSNDLPPKADLIVPFFKSVSYPLGVFGFISLTYVVIVGSSNAV
                     NLTDGLDGLAIMPVVMVGSALGLFAYVTGNVVYAKYLLLPHIPGAGELFVFCSAMAGA
                     GLAFLWFNAYPAQVFMGDVGALALGGALGTIAVIVRQEIVLAVMGGIFVVEALSVMAQ
                     VAWFKYTKKKYGEGRRILLMAPLHHHFEKSGWKETQVVVRFWIITMLLCLIGLSTIKL
                     R"
     gene            2130..>2867
                     /locus_tag="VFV25_13570"
     CDS             2130..>2867
                     /locus_tag="VFV25_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEY1394397.1"
                     /translation="MKHLYGLTVLVLGLGDSGLAMARWSARCGAQVRVADTREQPPQL
                     AALREHLPQASFSGGPFTASLLDGVNLVLKSPGLSPRDPAVAALLDQAAERGLLVQGE
                     LTLFARALADLKAERGYAPQLIAITGTNGKTTTTMMAGTLVARAGKRVAVAGNVGPTM
                     LQTLAEALDLEPEAQEQEPSPQPSTEAGEGAEPDRTPSPGTGEGGGEGTTVEAAPPED
                     EDAVPLQLAPPPPAAPRFEHLPEVWVLE"
BASE COUNT          441 a          955 c         1000 g          471 t
ORIGIN      
        1 gagctcggca tgaaccaccc tggcgagatc gctctgctgg cgcgctgggc cgcgccgacc
       61 atcgcgctgg tcaacaacgc gcagcgcgag caccaggagt tcatggcgac ggtcgaggcc
      121 gtagcgcgcg agaacggcac cgcgatccag gccctgtctg caaacggcat cgccgtcttc
      181 cccgccgacg acgcctacac gccggtctgg cgcgagctgg ccgcgcagcg caagacgctg
      241 agcttcgcgt tggatggatt gggcggcccg gccgatgtga gcggatcggc cgcctgggcc
      301 ggcgaccact ggcggttgga aatacgcacc ccagcgggca ccacaaacct gaagctgcgc
      361 gtggccggtg cgcacaacct gaagaacgcg ctggccgctg ccgcttgtgc actggcggca
      421 ggctgcccgc tcgaagcgat cgcgcgcggc ctggaagcct tcgagccggt caagggccgc
      481 tcgcagctgc agcgcctgaa gctcgccggc cgcgagctga cgctgatcga cgacagctac
      541 aacgccaacc ccgattcgat gcgtgcggcg atcgaggtgc tggccagcct gccggcccct
      601 cgctggctgg tgatgggcga catgggcgaa gtcggcgagc agggtccggc cttccaccgc
      661 gaggtgggca gctatgccgc gcagcgcggc atcgagcatc tgtgggcggc gggcgggcaa
      721 agcgcagccg cggctgcggc ctaccgcgag gcgggcggcg ccgatgtcag gcatttcgaa
      781 agcgccgcgg ccgtcgtggc cgcgctcgcg caggcacccg cggcagcgtc cgcggtgatc
      841 aagggatccc gcttcatgaa gatggaacaa gtggtggcag cactcaaggg agcgcaggat
      901 gctcgctagc ctgtcgggag ggaaacgcca tgctggttag cctggctcag tggctgcaat
      961 cggcctatcc cgagcagctc ggcttcctgc gcatcttcca gtacctgacg tttcgcgccg
     1021 tgatggcggc gatgacggcg ctgctgatcg gcctgacctt cggccccttc gtgatacgtc
     1081 gcctggccga gctcaagatc ggccagccga tccgcgagta cggcgtgcag tcgcacatgg
     1141 tcaagcgcgg cacccccacc atgggcggcg tgctgatcct gcttgcgatc gccgcgtcga
     1201 cgctgctgtg gttcgactgg agcaaccgct tcgtgtggat cgtgatgctg gtgaccaccg
     1261 gcttcggcgc gatcggctgg gtcgacgact ggcgcaaggt agtgcacaag aacccggaag
     1321 gaatgcgctc gcgcgagaag tatttctggc agtcggtgat cggcctcgtc gcggcgctct
     1381 acctcgcgtt cagcgtgtcg gagatctcca acctgcgcgt gctggagctc ttcctgcgct
     1441 gggtgcagag cggcttcagc aacgacctgc cacccaaggc cgatctgatc gtgcccttct
     1501 tcaagagcgt gagctatccg ctgggcgtgt tcggcttcat cagcctgacc tatgtcgtga
     1561 tcgtgggctc cagcaatgcg gtcaacctca ccgacggcct ggacggcttg gccatcatgc
     1621 cggttgtgat ggtgggctcg gcgctgggcc tctttgccta tgtgaccggc aacgtggtgt
     1681 atgccaaata cctgctgctg ccgcacatcc cgggcgccgg cgagctattc gtgttctgct
     1741 cggcgatggc cggcgcgggc ctggccttcc tctggttcaa cgcctacccg gcgcaggtct
     1801 tcatgggcga cgtgggcgcg ctggcgctgg gcggggcttt gggcacgatc gccgtgatcg
     1861 tgcgccagga gatcgtgctg gccgtgatgg gcggcatctt cgtcgtcgag gcgctgtcgg
     1921 tgatggccca ggtggcctgg ttcaagtaca cgaagaagaa atacggcgag ggccggcgca
     1981 tcctgctgat ggcgccgctg caccaccact tcgagaagtc cggctggaag gagacgcaag
     2041 tcgtcgtgcg cttctggatc atcaccatgc tgctgtgcct gatcggcctg tccaccatca
     2101 agctgcgcta agaccgtgaa gtatttgcca tgaagcatct gtacggtctc accgtcctcg
     2161 tcctcggcct cggcgactcg gggctcgcga tggcgcgctg gagcgcgcgc tgcggcgcgc
     2221 aggtgcgcgt ggccgacacg cgcgagcagc cgccccagct cgccgcattg cgcgagcatc
     2281 tgccgcaggc gagcttctcg ggcggtcctt tcacggccag cctgctcgac ggcgtgaatc
     2341 tggtgctgaa gagccccggg ctgtcgccgc gtgaccctgc cgtggccgcg ctgctcgacc
     2401 aggccgccga gcgcggcctg ctggtgcagg gcgaactgac tttgttcgcg cgcgcgctgg
     2461 ccgatctgaa ggccgagcgc ggctacgcgc cgcagctgat cgcgatcacc ggcaccaacg
     2521 gtaagaccac gaccacgatg atggccggca ccctggtcgc ccgcgccggc aagcgcgtgg
     2581 ccgtggccgg caatgtcggc ccgaccatgc tgcagaccct ggccgaggcc ttggatctgg
     2641 agcccgaggc gcaggagcaa gaaccctcac cccaaccctc taccgaagcg ggagaggggg
     2701 cagaacctga tcggactccc tctcccggga cgggagaggg cgggggtgag ggcaccacag
     2761 tcgaggccgc accgcccgag gacgaggacg ctgttccgct gcaactcgcg ccgccgccac
     2821 ccgccgcgcc gcgcttcgaa cacctgcccg aggtctgggt gctggag
//
LOCUS       DASUCY010000688         6026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_288703, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000688 DASUCY010000000
VERSION     DASUCY010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 6026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6026
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288703"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="VFV25_13575"
     CDS             <1..695
                     /locus_tag="VFV25_13575"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEY1394398.1"
                     /translation="GKRIAWLGDLQGYLQIEPGILEVCEQGLRRLEGLGCAIEPAALG
                     YPPERAWHTWLTLRHWIVTGRVAAYYADPAQREQLKPEARWEAEQGARLSANEIYAAS
                     LARTAFYQHLVGLFERFDLLALPSAQVWPFDAQWHWPRSINGRGMDTYHRWMEVVIYA
                     TLGGLPAISVPVGFSDAGLPMGMQLIGPPQADLPVLQAAAGYETTIGELLRRRPPDAL
                     SLQPSREARKTT"
     gene            692..1267
                     /locus_tag="VFV25_13580"
     CDS             692..1267
                     /locus_tag="VFV25_13580"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEY1394399.1"
                     /translation="MKRIVLVPLLLVAQALAMPAAQAAERAIDKQIVVAATLDQAWDA
                     WTTREGITSFFAPDAEIDARPGGAFHIYINPYAEPGMKGADEMRYMALQPKKMLSFDW
                     NAPPSLPEVRQQRTFVVLRFEPVGDKSTRITMHHTGWGEGGQWDQTYAYFDKAWGRVL
                     ANLQRRFETGPVDWTDWLQQMRKASAAAEKK"
     gene            1320..1721
                     /locus_tag="VFV25_13585"
     CDS             1320..1721
                     /locus_tag="VFV25_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEY1394400.1"
                     /translation="MFSHIFIGVSDFDRALAFYTALMGCLGNEARFCDRSRPWAGWQS
                     AGGGRPLFLIGKPYDGQPHERGNGQMVAFLAGSRAIVDRAYETALAHGGQSEGPPGLR
                     PEYHEHYYGAYFRDPDGNKLCVACHTPEPGR"
     gene            complement(1655..1924)
                     /locus_tag="VFV25_13590"
     CDS             complement(1655..1924)
                     /locus_tag="VFV25_13590"
                     /inference="COORDINATES: protein motif:HMM:NF019159.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HEY1394401.1"
                     /translation="MQLRSASAHADAAEIIGWLRTAPQAPREVFVTHGEPEAADALRQ
                     RIARELGWPAYVPIYRDSVDLLTGQRPGSGVWQATQSLLPSGSRK"
     gene            complement(1929..2102)
                     /locus_tag="VFV25_13595"
                     /pseudo
     CDS             complement(1929..2102)
                     /locus_tag="VFV25_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197002.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            2303..3076
                     /locus_tag="VFV25_13600"
     CDS             2303..3076
                     /locus_tag="VFV25_13600"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEY1394402.1"
                     /translation="MKTQLKLKQNLPDLASPGIEPVDDAAALGKEGWLSVLQASFHPM
                     APTAEAAQALLRLAKPLAVKRGDQLLSREQHADKFWLLLSGRVAMGVAEAGEMMQQTR
                     DIAPRQWIDNASALLGEDACYLEDALVEKDALVLSLACSDYLRCGAKHPSLMSGLAAV
                     LAERVSASLSTELSLIRQSIAQRLANWLLSHVEAGAQSGSIVLRQRKRAIASQLGTTP
                     ETLSRVLRMFITDGVIRVRGYTIDLLEIDSLRALAAARR"
     gene            3073..5391
                     /locus_tag="VFV25_13605"
     CDS             3073..5391
                     /locus_tag="VFV25_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEY1394403.1"
                     /translation="MSPVMAMNAAAAWDDPDELARFTRWTGAGSERRGESILVIEGMY
                     CAACSVGIEDALRGVAGVDSAEVNPATRRARMVWDPARTSASALIAAIEAAGYRAFPA
                     LSMQAEAQRRREQRAMLWRLFVAGFCAMQVMMYATPLYLSSSGDMSPDIRRLLLWASW
                     LLSIPVLSFSSGPFFRGAWQQLKRRRIGMDMPVALGIAVTFVASTGAAVDPGGPFGHE
                     VYFDSLTMFVFFLLCGRYLELRSRSATVGALEGLMLRLPEMAERLVGRDGAVELVPVR
                     RIKPGDRVRVRPGQGFPADGRLCDGPTQVDEALLSGESKPVLRCAGQAVMAGSFNLGS
                     PVVIEVERAGSDTRYAQVVALMERAAMDRPQLVRLADRFAGPFLWAVLLAALLAAVAW
                     SFIDPARAIWVAVAVLIVTCPCALSLATPSALLAAAGTLARGGVLVQRLAALEALARA
                     ELFVFDKTGTLTEDRLSLSIVNPAPGWTREAVLRQAATLAAGSLHPVSRALCEAVREE
                     ARDERAGPEALPLSGLREIAGSGVEARDADGRRWRLGSASFAGADAAPRGDEGVVAWL
                     SVDGAIVAGFAFDESLRADARMALAQLQADGVRVMLLSGDHSASAERVARSLGIREIH
                     AGASPEDKLRLLTEAQAGGLKVAMVGDGVNDGPVLARADVSIAMGQGAPLARAQADLT
                     LLSGRLADLVRARRLARRMLNIVRQNLAWAAVYNAACVPLALLGWLPPWLAGLGMACS
                     SLLVVLNAQRLRRTAEDAPQAARPVLAPLPQA"
     gene            5395..5622
                     /gene="ccoS"
                     /locus_tag="VFV25_13610"
     CDS             5395..5622
                     /gene="ccoS"
                     /locus_tag="VFV25_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HEY1394404.1"
                     /translation="MDILFLLVPMSVVLVLLIIVLFGWALHSGQFDNLEREGERILWD
                     QPLQRTKLDGDQPSAEPAPEQWRHRTEESRR"
     gene            5619..>6026
                     /locus_tag="VFV25_13615"
     CDS             5619..>6026
                     /locus_tag="VFV25_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEY1394405.1"
                     /translation="MSTAAAEIAAPAAHYNDKVVRQFTIMAVVWGIVGMLVGVFIAAE
                     LTWPELNMGIPWLSYGRLRPLHTNAVIFAFGGCALFATSYYVVQRTGQVRLFAEKLAS
                     FTFWGWQAVIVAAAVSLPLGYTRGKEYAELEWPI"
BASE COUNT          893 a         2041 c         2128 g          964 t
ORIGIN      
        1 acggcaagcg cattgcctgg ctcggcgacc tgcaaggcta tctgcagatc gagcccggca
       61 tcctggaggt ctgcgagcaa ggcttgcggc ggctcgaggg cctgggctgc gcgatcgagc
      121 cggcagccct gggctatccg cccgagcggg cctggcatac ctggttgaca ctgaggcact
      181 ggatcgtcac cggccgcgtc gcggcctatt acgccgatcc ggcgcagcgc gaacagctca
      241 agccagaggc gcgctgggag gccgagcagg gggctaggct cagcgccaac gagatctatg
      301 cggcgagcct ggcacgcacg gccttctatc aacacctggt gggcctgttc gagcgcttcg
      361 acctgctggc gctgcccagc gcccaggtct ggccttttga tgcgcagtgg cattggccgc
      421 gcagcatcaa cggccgcggc atggacacct atcaccgctg gatggaggtg gtgatctacg
      481 ccacgctggg cgggctgccg gccatcagtg tgccggtggg cttcagcgat gcaggcctgc
      541 cgatgggcat gcagctgatc ggcccgccgc aggccgacct gccagtgctg caggcggcgg
      601 cgggctacga gacgaccata ggcgaactgc tgcggcggcg cccgccggat gcgctttcct
      661 tgcagccatc gcgcgaagca aggaagacaa catgaaacgc atcgtcctgg tcccgctact
      721 gcttgttgcc caagccctcg ccatgcccgc ggcgcaggcc gccgagcgcg ccatcgacaa
      781 gcagatcgtg gttgccgcca cgctcgatca ggcctgggat gcctggacca cgcgcgaggg
      841 catcacgagt ttcttcgcgc cggacgccga gatcgatgcc cgccccggcg gcgccttcca
      901 catctacatc aacccctatg ccgagcccgg catgaagggc gccgacgaga tgcgctacat
      961 ggcgttgcag cccaagaaga tgctgtcctt cgactggaac gccccgccca gcctgcccga
     1021 ggtgcgccag cagcgcacct tcgtcgtgct gcgcttcgag ccggtgggcg acaagagcac
     1081 gcgcatcacg atgcatcaca ctggctgggg cgagggcggc caatgggacc agacctatgc
     1141 ctatttcgac aaggcttggg gcagggtgct tgccaatctg cagaggcgct tcgagacagg
     1201 acccgtcgac tggaccgact ggctgcagca gatgcgcaaa gccagtgcag ccgccgaaaa
     1261 gaaataggcc gcgagacaat ccgccccttc ctgttctccg caaaaacctg cgtctcagcg
     1321 tgttctcgca catcttcatt ggcgtcagcg acttcgatcg ggcgctggcc ttctacaccg
     1381 cattgatggg ctgcctcggg aacgaggctc gtttctgcga ccgcagccgc ccctgggccg
     1441 gctggcagag cgcaggggga ggcaggccgc tcttcctcat cggcaagccc tatgacgggc
     1501 agccgcacga gcgaggcaac gggcagatgg tcgccttcct cgcgggcagc cgggccatcg
     1561 tcgatcgggc ctacgagact gcgctggcac acggcggcca atcggaaggc ccgcccggtc
     1621 tcaggcccga atatcacgag cactattacg gggcctactt tcgagacccc gacggcaaca
     1681 agctctgcgt ggcctgccac acgccggagc ctgggcgctg accagtcagc aggtcgacgc
     1741 tgtcgcggta aatcggcaca taggccggcc atcccagctc gcgcgctatg cgctggcgca
     1801 gcgcgtcggc cgcctcgggt tcgccatgcg tgacgaagac ctcgcgcggc gcctgcggcg
     1861 ccgtgcgtag ccagccgatg atctcggcgg cgtcggcatg ggccgaggcg ctgcgaagct
     1921 gcaccaccga tgctcgaggc cggtaccggt agcggctccc agttgcgcag tcgcagcgtc
     1981 ttggagccct tgaacaggcc gcagtcgacg agcaagcgcc gctcgccggc ctcgaccaag
     2041 tatttggagc cggtgacggt gccgacggca ccgaggaagc tcaggctgaa cacggacggc
     2101 atgggcggcg cactctcatc gagcgatctg caggctagcg ctacatcttg ggccgcccgc
     2161 cggtcggccg tcttgatcga tatcaaggct tcgcgctgcg cgtggcattg atgcatgtca
     2221 atacgccggc ccgaggtcgg cgacaagatc gccgcggtca ccgtcgccac gtcgcgtcga
     2281 caggccccgc gcacggcagc ccatgaagac tcaactcaag ctcaaacaga accttccgga
     2341 cctggcctcg cctggtatcg agccggtgga cgacgccgct gcgcttggca aagagggctg
     2401 gctgagcgtc ctgcaggctt ccttccatcc gatggcgcca accgccgagg cggctcaagc
     2461 gctgctgcgg ctggccaagc ctctcgccgt gaagcgcggc gaccagctgc tgtcgcgcga
     2521 gcagcatgcc gacaagttct ggctgctgtt gagcgggcgc gtggccatgg gcgtggccga
     2581 ggccggcgag atgatgcagc agacacgcga catcgctccg aggcaatgga tagacaacgc
     2641 cagcgcgctg ctgggcgagg atgcctgcta cctggaagac gcgctggtcg agaaggacgc
     2701 gctggtcctg tctctggcgt gcagtgatta cctgcgctgc ggtgccaagc atccttcgct
     2761 gatgagcggc ctggccgcag tgctggccga gcgcgtaagc gcgagtcttt cgaccgagct
     2821 gagcctgatc cggcagagca tcgcacagcg cctggcgaac tggctgttga gccatgtcga
     2881 agcaggggcg caatccggca gcatcgtgct gcgccagcgc aagcgcgcca tcgcctccca
     2941 gctgggcacc acgccagaga cgctgtcgcg cgtgctgcgc atgttcatca ccgacggggt
     3001 gatcagggtc aggggctaca cgatagacct gctcgagatc gacagcctgc gggcgctcgc
     3061 ggctgcgcga cggtgagccc cgtgatggcg atgaacgccg cggccgcctg ggacgatccc
     3121 gacgaacttg cccgcttcac gcgctggact ggcgccggca gcgagcgccg cggcgaatcc
     3181 attctcgtca tcgaaggcat gtactgcgcg gcctgttcgg tcggcatcga ggacgcgctg
     3241 cgcggcgtgg cgggcgtcga ttcggccgag gtcaatcctg cgacgcggcg cgcccgcatg
     3301 gtctgggatc cggcgcgcac cagcgcatcg gccctcatcg ccgccatcga ggcggccggc
     3361 taccgcgcct tcccggcgct gtcgatgcag gccgaggcgc agcgccggcg cgagcagcgc
     3421 gcgatgctgt ggcgcctgtt tgtagccggc ttctgcgcga tgcaggtcat gatgtatgcg
     3481 acgccgctct atctctccag cagcggcgac atgagccccg acatcaggcg gctgctgctg
     3541 tgggccagct ggctgctcag catcccggtg ctgagcttct cttccggccc cttcttccgc
     3601 ggcgcctggc agcagctcaa gcgccggcgc attggcatgg acatgccggt ggcgctgggc
     3661 atcgccgtca ccttcgtcgc cagcacgggc gcagcggtcg acccgggcgg cccgttcggg
     3721 cacgaggtct atttcgactc gctgacgatg ttcgtgttct tcctgctgtg cgggcgctat
     3781 ctcgaactgc gctcgcgcag cgccaccgtc ggcgcgctcg aaggactgat gctgcgcctg
     3841 ccggagatgg cggaacggct ggtgggccgc gacggcgcgg tggagctggt gccagtgcgc
     3901 cgcatcaagc cgggcgaccg ggtacgggtg cgcccgggcc agggctttcc ggccgacggc
     3961 cggctgtgcg atggtccaac ccaggtcgac gaggccctgc tcagcggcga atcgaagccg
     4021 gtgctgcgct gcgccggtca ggcggtgatg gcgggcagct tcaatctcgg ttcgccggtc
     4081 gtgatcgagg tggagcgcgc cggcagcgac acgcgctatg cgcaggtcgt cgcgctgatg
     4141 gagcgtgccg ccatggaccg gccgcagctg gtgcggctgg ccgatcgctt cgccggcccc
     4201 ttcctgtggg cggtgctgct ggcggccttg ctggcggccg tagcctggtc cttcatcgat
     4261 ccggcgcgcg ccatctgggt ggcggtggcg gtgctgatcg tgacctgccc ctgcgcgctg
     4321 tcgctggcca cgccttcggc gttgctggcc gctgccggca cgctggctcg cggcggcgtg
     4381 ctggtgcagc gcctggcggc cctcgaagcc ctggcgcgcg ccgagctctt cgtcttcgac
     4441 aagaccggca ccctgaccga ggacaggctg agcctgagca ttgtcaatcc ggcaccgggg
     4501 tggacgcgcg aggcggtctt gcgccaggcc gcgaccttgg cggcgggctc gctgcatccg
     4561 gtgtcgcggg cgctgtgcga agcagtgcgc gaagaggcgc gcgacgagcg ggccgggcca
     4621 gaggcgctgc cgctgtctgg cctgcgcgaa atcgcgggca gcggggtcga ggcccgcgat
     4681 gccgacgggc gccgctggcg cctgggctcg gcctcttttg cgggcgcaga tgcggcaccg
     4741 cgaggggacg agggcgtcgt cgcctggctg tcggtggacg gcgcgatcgt cgccggcttc
     4801 gccttcgatg agagcctgcg cgccgatgcg cgcatggcgc tggcccagct gcaggccgac
     4861 ggcgtgcgcg tgatgctgct gtccggtgac catagcgcct cggccgagcg cgtggcgcgc
     4921 agcctgggca tccgcgagat ccacgccggc gcctcgcccg aggacaagct gcgcctgctg
     4981 accgaagcgc aggccggcgg cctcaaggtg gcgatggtgg gcgacggcgt caatgatggc
     5041 ccggtgctgg cgcgcgccga tgtctcgatc gcgatgggcc agggtgcacc gctggcgcgc
     5101 gcccaggccg atctgaccct gctcagcggc cgcctcgcgg acctggtgcg cgcgcggcgg
     5161 ctggcgcggc gcatgctgaa catcgtgcgc cagaacctcg cctgggccgc cgtctacaac
     5221 gcggcctgcg tgccgctcgc gctgctcggc tggctgccgc cctggctggc cgggttgggc
     5281 atggcctgca gctcgctgct cgtggtcctg aacgcgcagc gcctgcggcg caccgccgag
     5341 gatgcgccgc aggctgcgcg accggttctc gctccgttgc cgcaggcctg agccatggac
     5401 atcctcttcc tgctcgtgcc gatgtcggtg gtgctggtgc tgctgatcat cgtgctgttc
     5461 ggctgggccc tgcacagcgg ccagttcgac aacctggagc gcgagggcga acgcatcctg
     5521 tgggaccagc cgctgcagcg cacaaagctt gatggcgatc aacctagcgc ggaacccgcg
     5581 ccggaacaat ggcgccaccg aaccgaggag agccgtagat gagcaccgcc gccgccgaga
     5641 tagccgcgcc agccgcgcat tacaacgaca aagtggtgcg ccagttcacc atcatggcgg
     5701 tggtgtgggg catcgtcggc atgctggtgg gcgtgttcat cgccgccgag ctgacctggc
     5761 ctgagctgaa catgggcatc ccctggttga gctacgggcg gctgcgtccg ctgcacacca
     5821 atgcggtgat cttcgccttc ggcggctgcg ctctcttcgc caccagctat tacgtcgtgc
     5881 agcgcacggg ccaagtgagg ctgttcgcag agaagctcgc gtccttcacc ttctggggct
     5941 ggcaggcggt gatcgtggcc gccgcggtct cgctgccgct gggctacacc cgcggcaagg
     6001 aatacgccga gctcgaatgg ccgatc
//
LOCUS       DASUCY010000689         2422 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_112444, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000689 DASUCY010000000
VERSION     DASUCY010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112444"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="VFV25_13620"
     CDS             <1..659
                     /locus_tag="VFV25_13620"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEY1394406.1"
                     /translation="RLAGIGARHRDFGRSALLVPLAHERDHQGLAQECFLEGHTLALL
                     PMPGRRSSAVWTVTNAALPAILAMDDAQLAATIEAAAEHRLGAMRVDGSRQVYPLVAV
                     WAERFAAQRLALIGDAAVGMHPVTAHGYNLGLYGVEALSRRLQPGRDAGELAPLLAYE
                     AEHRRVAGPLYFGTNALVRFFTDDRTPVLVLRRALIDVARHLPPLRAAITRRLTDAAA
                     "
     gene            complement(710..1450)
                     /locus_tag="VFV25_13625"
     CDS             complement(710..1450)
                     /locus_tag="VFV25_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEY1394407.1"
                     /translation="MAIKVLIVEDKPVARTFLCRVVRESFSDELDITEAGDLESARGK
                     LGLRPGAPSVAGTVPYKLVLIDLELPDGNGLELLSQLAKCSAVKVVTTLYSDDDHLFP
                     ALQCGADGYLLKEDRFEVLVEELQKIVRHQPPLSPAIARRLLSHFRLGEGSSGLKDSS
                     FSALDSPSGGGPIPLGRNDPPDHERLSPRENEVLTYLSKGFTIKEIANLMGIKWFTVN
                     DHIKTIYKKLNVSSRAEAAVVASKQGLV"
     gene            complement(1573..>2422)
                     /locus_tag="VFV25_13630"
     CDS             complement(1573..>2422)
                     /locus_tag="VFV25_13630"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HEY1394408.1"
                     /translation="LLIVQNKYQMKPPLPFVPGAEFSGVVEAVGEGVKHLAPGMAVAS
                     FGGIGGFATQVCVNAALVMPLPPGFDLADAAAFLCTYGTSHHALMDRAALRAGETVLV
                     LGAAGGVGTAAIQIAKAAGARVIAAASSDEKCELCRRLGADAAINYQREDLREALKAA
                     TNGKGPDVIYDPVGGDLAEPAFRSIGWRGRYLVIGFAGGNIPALPLNLALLKGASIVG
                     VFWGEFARREPANNAKALAELAAWYAQGKIKPVIEQRLSMHELPRAFELMGQRQVRGK
                     LVLVND"
BASE COUNT          367 a          804 c          832 g          419 t
ORIGIN      
        1 gccgcctggc gggcataggg gctcgccatc gcgacttcgg gcgcagcgct ctgctcgtgc
       61 cgctcgccca cgagcgcgat catcaaggcc tggcccagga atgctttctc gaaggccaca
      121 cgctggcgct cctgcccatg ccgggacggc gcagctcagc agtgtggacc gtgaccaatg
      181 cggcgttgcc ggcaatcctg gccatggacg acgcgcaatt ggccgcgacc atcgaagcgg
      241 ccgccgaaca ccgcctgggc gccatgcggg tggatggctc gcgccaggtc tatcccttgg
      301 tggccgtctg ggccgagcgc ttcgctgctc agaggctggc tctcattgga gacgccgccg
      361 tgggcatgca cccagtgacg gcccatggct acaacctcgg gctctacggc gtggaagccc
      421 tgagccggcg cctgcagccg gggcgcgacg cgggcgagtt ggcgcctctg ctggcctacg
      481 aggctgagca ccgccgcgtg gccgggccgc tctacttcgg caccaatgcg ctggtgcgct
      541 tcttcaccga cgaccgtacg ccggtgctgg tcctgcgccg cgcgctgatc gatgtggcgc
      601 ggcatttgcc gccgctgcgg gccgccatca cgaggcggct cactgatgct gcggcttgat
      661 caggcagtgc gagttttttc ttgcgcgtgc gcagtggttg cggtgcgcgt cagaccagcc
      721 cttgcttgct ggccaccacc gccgcctcgg cccggctgga cacgttgagc ttcttgtaga
      781 tggtcttgat gtggtcgttg acggtgaacc acttgatgcc catgaggttg gcgatctcct
      841 tgatcgtgaa gcccttgctc aagtaggtca gcacctcgtt ctcgcgcggg ctcaggcgct
      901 cgtgatcagg cgggtcgttc cggcccaggg ggatgggacc gccgccgctg gggctgtcca
      961 gggccgagaa gctcgagtcc ttgagaccgc tgctgccttc accaagacgg aagtgggaca
     1021 gcaggcggcg cgcgatggcc ggcgacagcg ggggctgatg gcgcacgatc ttttgcaatt
     1081 cctcgaccag tacctcgaag cggtcttcct tcagcagata gccgtcggcg ccgcactgca
     1141 gggccgggaa gaggtgatcg tcgtcggaat agagcgtggt gacgaccttg acggccgagc
     1201 acttggccag ctggctcaag agctccagcc cattgccgtc cggcagctcg aggtcgatca
     1261 gcaccagctt gtagggaact gtgcctgcga cggagggcgc tccgggacgc agacccaact
     1321 tgccgcgtgc agattccaga tcgccggctt cggtgatgtc gagctcatcg ctgaagctct
     1381 cgcgtaccac cctgcacagg aaggtgcggg cgactggttt gtcttcgacg atcaggacct
     1441 tgattgccat ggtctgcgaa ctccctgggc cgtatcgggc gatggatggt gctgggctcc
     1501 acccaacacg aaattatcca cgccctgtca tcggcgcgtc cggatcgtcg aaacaggacc
     1561 tgatcgcggt gctcagtcgt tgaccagcac cagcttgccg cgcacctggc gctgccccat
     1621 cagctcgaag gcgcgcggca gctcgtgcat ggacaggcgc tgctcgatca ccggcttgat
     1681 cttgccctgg gcataccagg ccgccagctc ggccagcgcc ttcgcgttgt tggcgggctc
     1741 gcggcgcgca aactcgcccc agaacacgcc gacgatggat gcgcccttga gcagcgcgag
     1801 gttgagcggc agggccggga tgttgccgcc cgcgaagccg atgaccaggt agcggccgcg
     1861 ccagccgatg gagcggaagg ccggctcggc cagatcgccg cccacggggt cgtagatcac
     1921 gtcggggccc ttgccattgg tcgcggcctt gagcgcctcg cgcaggtctt cgcgttggta
     1981 gttgatcgcc gcgtcggcgc ccaggcgccg gcacagctcg cacttctcgt cgctggaggc
     2041 ggcggcgatg acacgtgcgc cggcggcctt tgcgatctgt atcgccgcgg tgcccactcc
     2101 gcctgcggcg cccaacacca gcacggtttc gccggcgcgc aaggcggcgc ggtccatcag
     2161 cgcatggtgg ctggtgccat aggtgcacag aaaggcggct gcatccgcga ggtcgaagcc
     2221 tggcggcagc ggcatcacca gggcggcgtt cacgcaaacc tgggtcgcga agccgccgat
     2281 gccgccgaag gaggcgacgg ccatgccggg cgccagatgc ttgacgcctt cgcccaccgc
     2341 ctcgacgacg ccggagaact cggcacccgg cacgaagggc agcggcggtt tcatctggta
     2401 cttgttctgc acgatcagca ga
//
LOCUS       DASUCY010000690         2412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_481760, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000690 DASUCY010000000
VERSION     DASUCY010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481760"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..141)
                     /locus_tag="VFV25_13635"
     CDS             complement(<1..141)
                     /locus_tag="VFV25_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEY1394409.1"
                     /translation="MKAIWNGTVVAESDDTVVVEGNHYFPESTVKREYLLPSNTKTMC
                     SWK"
     gene            complement(271..1275)
                     /gene="egtD"
                     /locus_tag="VFV25_13640"
     CDS             complement(271..1275)
                     /gene="egtD"
                     /locus_tag="VFV25_13640"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006763030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEY1394410.1"
                     /translation="MKNPNLLRATPDLRALPQRQTSARDEAAGSDFAQAMLQALSQRP
                     RSIAPKFFYDARGSALFDAICQLPEYYPTRTELGLLAAHAGEMARSIGAQAEIVEFGA
                     GSSRKVRLLLDALEQPRRFLPIDISGEHLQAAMAPLRRDYPALDVQVVVGDFTRELEL
                     PAVQPGARRVGFFPGSSIGNFTPEEARDFLRHAAVLLRGGGLLIGVDLVKDPGLLHAA
                     YNDAAGVTAAFNLNLLARANRELGTDFDLAQFAHYAFYEPRQQRIEMHLLSRRAQAVN
                     FGGQRFEFDEGESLHTENSYKYTVEGFQALAAQAGFEPGPVWCDAQRLFSVHWLEAAA
                     "
     gene            complement(1272..>2412)
                     /gene="egtB"
                     /locus_tag="VFV25_13645"
     CDS             complement(1272..>2412)
                     /gene="egtB"
                     /locus_tag="VFV25_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HEY1394411.1"
                     /translation="HTSWFFETLVLMPHARGYRAHDERYQFLFNSYYESLGPRHPRPQ
                     RGLLTRPSADEVLRNREKVDEAVAHLIETASAGQWAEAVPLLMLGLQHEQQHQELMLA
                     DIKHLLSLNPLQPAYIERPAHAVRVGASKAAPNEWLHFGGGVVEIGHDGAGFAFDNET
                     PRHQVLLRPYELASRPVSCGEYLAFVRDGGYRRPELWLSDGWATVQAQGWAAPLYWSE
                     VDAEQPTVFTLFGPQPLQAAEPVCHLSLFEAAAYAAWAGARLPTEFEWEAAAERCADD
                     ASPRYEPEHPHPRAADEETQPGLQQMLGEVWEWTRSSYDPYPGFRPLAGAVGEYNGKF
                     MVGQNVLRGGSCASPPGHLRASYRNFFPPAARWQFSGLRLARDAA"
BASE COUNT          385 a          862 c          780 g          385 t
ORIGIN      
        1 cttccacgag cacatggtct tggtgttgct gggcagcagg tactcgcgct tgaccgtgct
       61 ttcggggaaa tagtgattgc cttcgaccac cacggtgtcg tcgctctcgg cgaccaccgt
      121 gccgttccag attgccttca tcgtcatcct ttcactcttg acagcccctc gtccgacgag
      181 tacgtcggcc ggtccccagg ggccacttgg ggccccttcg tggcccttgg gacgcgggcc
      241 ggcttgggag cggcccggcg ctcggcccgc tcacgccgcg gcctcgagcc agtgcacgct
      301 aaacagccgc tgtgcatcgc accacacagg cccgggctcg aagccggcct gcgccgccag
      361 cgcctggaaa ccctccaccg tgtacttgta ggagttctcg gtgtgcaggc tctcgccctc
      421 gtcgaattcg aagcgctggc caccaaagtt cacggcctgc gctcggcggc tcagcagatg
      481 catttcgatg cgctgctggc gcggctcgta gaaggcgtaa tgcgcaaact gcgcgaggtc
      541 gaagtcggtt cccagctcgc ggttcgcgcg cgccagcagg ttgagattga atgccgccgt
      601 cacacccgcc gcatcgttgt aggccgcatg cagcagaccc gggtccttca ccagatcgac
      661 gccgatcaac agcccgccgc cgcgcagcag caccgcggcg tggcgcagga aatcacgcgc
      721 ctcctcgggc gtgaaattgc cgatcgacga gccagggaag aagcccacgc gccgcgcgcc
      781 cggctgcacc gccggcagct ccagctcgcg cgtgaagtcg ccgacgacga cctgcacgtc
      841 cagcgccgga tagtcgcgcc gcagcggcgc catcgccgcc tgcagatgct cgcccgagat
      901 gtcgatcggc agaaagcgcc gcggctgctc cagcgcatcg agcagcagcc tcaccttgcg
      961 gctggagccg gcgccgaact cgacgatctc ggcctgcgct ccgatgctgc gcgccatctc
     1021 gccggcatgg gcggcgagca ggccgagctc ggtgcgcgtc gggtagtact ccggcagctg
     1081 gcagatcgca tcgaacaatg ccgagccgcg cgcgtcgtag aagaacttgg gcgcgatgct
     1141 gcgcggccgc tgcgacaagg cctgcagcat cgcctgtgcg aagtcgctgc ccgcggcttc
     1201 gtcgcgcgcg ctcgtctgcc gctgcggcag cgcgcgcaga tcgggcgtgg cccgcagcag
     1261 attcgggttc ttcatgcggc atcccttgcc aggcgcaagc cggagaactg ccagcgtgcc
     1321 gccggcggaa agaaattgcg atagctcgcg cgcagatggc caggcggcga ggcgcagcta
     1381 ccgccgcgca gcacattctg gcccaccatg aacttgccgt tgtactcgcc caccgcaccg
     1441 gccaggggcc tgaagccggg atagggatcg taggacgagc gcgtccattc ccatacctcg
     1501 cccagcatct gctgcaggcc cggctgcgtc tcttcgtcgg ccgcacgcgg atgcgggtgc
     1561 tcgggttcgt agcgcgggct tgcatcgtcg gcacaacgct cggccgccgc ttcccactcg
     1621 aactcggtcg gcagtcgtgc accagcccag gctgcatagg ctgccgcctc gaacaggctc
     1681 aggtggcaga caggctcggc cgcttgcaag ggctgcggcc cgaacagcgt gaacacggtc
     1741 ggctgttcgg catccacctc gctccaatac agcggcgcgg cccagccctg agcctgcacc
     1801 gtggcccagc catcgctgag ccagagctcg gggcggcgat agccgccatc acgcacgaag
     1861 gccaggtatt ccccgcagct caccggccgg ctcgccagct cgtagggccg cagcagcacc
     1921 tggtggcgcg gcgtctcgtt gtcgaaggcg aagcctgcgc catcgtggcc gatctcgacc
     1981 acgccgccgc cgaaatgcag ccactcgttc ggcgccgctt tcgatgcgcc cacccgaacg
     2041 gcatgcgccg gcctctcgat atatgccggt tgcagcgggt tgagcgacag cagatgcttg
     2101 atgtcggcca gcatcagttc ctgatgctgc tgctcgtgct gcaggcccag catcaacagc
     2161 gggacggcct cggcccactg gcccgcggac gccgtttcga tcaaatgcgc caccgcctca
     2221 tccaccttct cgcggttgcg cagcacctcg tccgccgaag gccgcgtcag caagccgcgt
     2281 tgcggccgcg gatgccgcgg gccgagcgat tcgtaatagg aattgaagag gaattgatag
     2341 cgctcgtcat gcgcccggta gccgcgcgca tgcggcatca gcaccagcgt ctcgaagaac
     2401 cagctcgtgt gc
//
LOCUS       DASUCY010000691         2491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_144507, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000691 DASUCY010000000
VERSION     DASUCY010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144507"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..420)
                     /gene="rpsO"
                     /locus_tag="VFV25_13650"
     CDS             complement(151..420)
                     /gene="rpsO"
                     /locus_tag="VFV25_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEY1394412.1"
                     /translation="MSIADLNKAEIVKSNARGAADTGSPEVQVALLTARINELTPHFK
                     THLKDHHGRRGLLKMVSRRRKLLDYLKNKDADRYTALIQKLGLRK"
     gene            complement(568..1719)
                     /locus_tag="VFV25_13655"
     CDS             complement(568..1719)
                     /locus_tag="VFV25_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEY1394413.1"
                     /translation="MRDVIRQLPGSKIREVANAGMGRSDVLAFWFGESDEVTPAFIRE
                     AAAESLARGETFYAHNLGLPELREALAAYTSALHPRVGSERIAVTSSGVSALMIAMQA
                     LAGAGDEVVVVVPVWPNLTAQPAILGAQVTRVSLHPRSGAWRLDLDELLAHVTTKSRV
                     LLLNAPNNPTGWCLSREEQAAILAHCRRTGTWIVADEVYERLYFDGQRRAAPSFLDVA
                     EPGDRLIVVHSFSKSFLMTGWRLGWMVLPPGLAEDIGKLIEFNTSCAPVFVQRAGLAG
                     LQQADRFVPELVARLRACRDTLLPRLAALPGVEVAVPSGGMYAFFRVAGQGDSLAYAK
                     RLVAEVGLGLAPGAAFGPEAEGWLRWCFASRDPQRLAQGVERLQRSTAL"
     gene            complement(1740..>2491)
                     /locus_tag="VFV25_13660"
     CDS             complement(1740..>2491)
                     /locus_tag="VFV25_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY1394414.1"
                     /translation="VKKVYLIGQDYSFGRYVLKQGRALIAAKRPDVEVVGEELHPIGR
                     IKDFLPYATKIKASGADAVLTGNWGNDLTLLIKAAREAGVDAKFYTFYGNALGAPTAI
                     GEAGVGRVLAVAEWHPNVGTPGSDRFYQSFRQRFPRPEDDYVHARMQAMIELLAQAME
                     KARSTDAVAVARALEGLRLDARAIYGFHEGWMRAADHQFIQPLYVSVMERAGSPGVRF
                     DNEGSGYGFRTLRHFEPVATELPPHCAMRRF"
BASE COUNT          419 a          854 c          822 g          396 t
ORIGIN      
        1 agcccggcac ctgagcggta tgccattcca gcgcggctgt acccagcagc caccctggaa
       61 tgacacagca acactcgcat cgtgtccggc tccaaagaca aagagcctgt gctggcagca
      121 ccagacaggc tcttgttcat ctcggcaacc ttacttgcgc aggcccagct tctggatcag
      181 cgcggtgtag cggtcggcgt ccttgttctt gaggtagtcc agcagcttgc ggcggcggct
      241 caccatcttc agcaggccac ggcggccgtg gtgatccttc agatgggtct tgaagtgcgg
      301 cgtcagctcg ttgatgcggg cggtcagcag ggccacctgc acttccgggg agccggtgtc
      361 ggcggcgccg cgggcgttgg acttgacgat ctcggctttg ttgaggtcgg cgatggacat
      421 gatggtgtcg atttcctatg gcagtgaaac cggcacctcg tgcctgacat cggcgcaaga
      481 gtggacggtt tcgttttcac ttgcggtcgc gggtgaaacc tacccgcgcc gtgctgtttt
      541 ccacgccgtt cagcgaggaa gcaatgatta tagcgccgtg gagcgctgca ggcgctccac
      601 cccctgcgca aggcgctgcg ggtcgcgcga ggcaaagcac cagcgcaacc agccctcggc
      661 ctccggcccg aaggcggccc ccggcgccag ccccaggccg acctcggcga ccagccgctt
      721 ggcataggcc agcgaatcgc cttgccctgc cacgcggaag aaggcataca tgccgccgga
      781 cggcacggca acctcgacgc cgggcagcgc ggccaggcgc ggcagcaagg tgtcgcggca
      841 ggcccgcagc cgcgccacca gctccggcac gaagcggtcg gcttgctgca ggccggccag
      901 cccggcacgc tgcacgaaga ccggcgcgca cgaggtgttg aactcgatga gcttgccgat
      961 gtcctcggcc agcccgggcg gcagcaccat ccagcccagg cgccagccgg tcatcaggaa
     1021 gctcttggaa aagctgtgca caacgatgag gcgatccccg ggctcggcca cgtcgaggaa
     1081 ggacggtgcg gcccgccgct gcccgtcgaa gtacaagcgc tcgtagacct cgtcggcgac
     1141 gatccaggtg ccggtgcgcc ggcaatgcgc gaggatggcg gcctgctcct cgcggctcag
     1201 gcaccagccg gtcgggttgt tgggcgcgtt gagcagcagc acgcgactct tagtcgtcac
     1261 gtgggccagc agttcatcca ggtcaagccg ccaggcgccg gagcgcggat gcaaagacac
     1321 acgcgtcacc tgcgcaccca ggatggccgg ctgcgccgtc agattgggcc ataccggcac
     1381 cacgaccacc acctcgtcgc ccgcacccgc cagggcctgc atggcgatca tcagcgcgct
     1441 cacgccggac gaagtcacgg cgatgcgctc gctgcccacg cgcggatgca aggcgctcgt
     1501 ataggccgcc agcgcctcgc gcagctcggg caggccgagg ttgtgggcat agaaggtctc
     1561 gccgcgcgcc agcgattcgg cggccgcctc gcggatgaag gccggcgtca cctcgtcgct
     1621 ctcgccgaac cagaaggcca gcacatcgct gcgccccatg ccggcattgg cgacttcacg
     1681 gatcttggag ccgggtaact gacgaatgac gtcgcgcatg aaaacttctc cctgcttgat
     1741 cagaacctgc gcatcgcgca atgcggcggc agctcggtgg ccacgggctc gaagtggcgc
     1801 agcgtgcgaa agccatagcc cgagccctcg ttgtcgaagc gtacgcccgg gctgccggcg
     1861 cgctccatca cgctgacgta gagcggctgg atgaactggt gatccgccgc acgcatccag
     1921 ccttcatgaa agccgtagat cgccctggcg tcgagcctga ggccttcgag cgcgcgggcc
     1981 acggccaccg catcggtgct gcgcgccttc tccatcgctt gcgccagcag ctcgatcatg
     2041 gcctgcatgc gcgcatgcac atagtcgtcc tcgggccggg gaaagcgctg gcggaagctc
     2101 tggtagaagc ggtccgagcc cggcgtgccg acgttgggat gccattcggc caccgccagc
     2161 acgcggccca cgccggcctc gccgatggcc gtcggtgcgc ccagggcatt gccgtagaag
     2221 gtgtagaact tcgcgtcgac gccggcctcg cgcgcggcct tgatcaacag cgtgaggtca
     2281 ttgccccagt tgcccgtcag cacggcgtcg gcgccgctgg ctttgatctt ggtcgcatag
     2341 ggcaggaaat ccttgatgcg gccaatcggg tgcagctcct cgccgacgac ttctacatcc
     2401 ggccgcttgg ccgcaatcag cgcgcggccc tgcttcagca catagcggcc gaagctgtag
     2461 tcctggccga tcagatagac cttcttcacc g
//
LOCUS       DASUCY010000692         2973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_578043, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000692 DASUCY010000000
VERSION     DASUCY010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578043"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="VFV25_13665"
     CDS             <1..1057
                     /locus_tag="VFV25_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006386387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEY1394415.1"
                     /translation="GRAVGPWDPKADPQLLRRGLTAMMRTRIFDARMLIAQRQKKISF
                     YMQCLGEEAIATAHALALQPGDMCFPTYRQQGLLLARDDVDMVELMCQLMSNERDPLK
                     GRQLPVMYSYKRAGFFTISGNLATQFIQAVGWGMASAIKGDTKIASGWIGDGATAEAD
                     FHTALTFAHVYRAPVILNVVNNQWAISTFQAIAGGEATTFAARGVGCGIASLRVDGND
                     FLAVLAASRWAAERARSNLGPTLIEWVTYRAGAHSTSDDPSKYRPADDWQRFPLGDPI
                     ARLKQHLVLIGAWSEAEHEAAQKELEAEVAAAQKEAERYGTLIDGHIPPLASMFEDVY
                     KDMPAHLHEQLRQAGGA"
     gene            1060..2073
                     /locus_tag="VFV25_13670"
     CDS             1060..2073
                     /locus_tag="VFV25_13670"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEY1394416.1"
                     /translation="MAQMSMIQALRSAMDVMLERDPNVVIFGQDVGYFGGVFRCTEGL
                     QAKYGKSRVFDAPISEGGIVGSAVGMGAYGLRPVVELQFADYMYPAYDQIVSEAARLR
                     YRSAGDFTAPLTIRMPCGGGIYGGQTHSQSPEGLFTHVCGLRTVMPSNPYDAKGLLIA
                     SIENDDPVIFLEPKRLYNGPFDGHHDKPVVPWSKHALGEVPEGHYTVPLDTAKIFRPG
                     KDLTVLAYGTMVFVCEAAAIESGIDAEIIDLRSLWPLDLDTIVASVKKTGRCVVVHEA
                     TRTSGFGAELASLVQEHCFYHLEAPIERVTGWDTPYPHALEWAYFPGPARVAAAYKRA
                     MED"
     gene            2115..>2973
                     /locus_tag="VFV25_13675"
     CDS             2115..>2973
                     /locus_tag="VFV25_13675"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011545041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEY1394417.1"
                     /translation="MGIHVIKMPDIGEGIAEVELVAWHVKPGDRVVEDQVLADVMTEK
                     ASVEIPSPVAGEVMALGGEIGQVLAVGSELIRIEVEGAGNVKAAASAAAAASAKALAP
                     APAAKPAAAANKPASPTALVPVPSLPAHEPARPLYADRPIASPSVRRRAWELGVDLAQ
                     VPASGTAGRIMQADLDAYAARDSGARPATRPTASLIPLAPRGVARRDEEAIKLIGLRR
                     KIAQKMQEAKRRIPHFSYVEEVDVTELEALRARLNAKWGAQRPRLTVLPFLVRALVLS
                     LREHPEINAR"
BASE COUNT          484 a         1030 c         1012 g          447 t
ORIGIN      
        1 cggacgcgcc gtcggcccgt gggatccgaa ggccgatccg cagctgctgc gccgcgggct
       61 caccgccatg atgcgaacgc gcatcttcga cgcgcgcatg ctgatcgcgc agcggcagaa
      121 gaagatctcg ttctacatgc aatgcctggg cgaggaggcg atcgccaccg cccatgcgct
      181 ggccttgcag ccgggcgaca tgtgcttccc cacctaccgc cagcagggcc tgctgctggc
      241 gcgcgacgac gtcgacatgg tggagctgat gtgccagctg atgagcaacg agcgcgatcc
      301 gctcaagggc cgccagctgc ccgtcatgta ttcctacaag cgcgcgggct tcttcacgat
      361 cagcggcaat ctcgcgaccc agttcatcca ggcggtgggc tggggcatgg cctcggcgat
      421 caagggcgac accaagatcg cctcgggctg gatcggcgac ggcgcgacgg ccgaggccga
      481 cttccacacc gcgctgacct tcgcccatgt ctaccgcgcg ccggtgatcc tcaacgtggt
      541 caacaaccag tgggcgatct cgaccttcca ggcgatcgcc ggcggcgagg ccaccacctt
      601 cgcggcgcgc ggcgtgggct gcggcatcgc ctcgctgcgc gtggacggca acgacttcct
      661 cgccgtgctg gccgcctcgc gctgggcggc cgagcgcgcg cgcagcaatc tggggccgac
      721 gctgatcgag tgggtcacct accgcgccgg cgcccattcg acctcggacg acccgtccaa
      781 atatcgcccg gccgacgact ggcagcgctt tccgctcggc gatccgatcg cgcggctgaa
      841 gcagcatctc gtcctcatcg gcgcctggtc cgaggccgag cacgaggcgg cgcagaagga
      901 gctggaggcc gaagtggccg cagcgcagaa agaggccgag cgctacggca cgctgatcga
      961 cggccatatc ccgccgctgg cctcgatgtt cgaggacgtc tacaaggaca tgcccgcgca
     1021 tcttcacgag cagctgcgcc aggcaggggg agcctgagca tggcccagat gagcatgatc
     1081 caggccctgc gttccgcgat ggacgtgatg ctggagcgcg accccaacgt cgtgatcttc
     1141 ggccaggatg tcggctattt cggcggcgtc ttccgctgca ccgaggggct gcaggccaag
     1201 tatggcaagt cgcgcgtgtt cgatgcgccg atctccgaag gcggcatcgt gggctcggcg
     1261 gtgggcatgg gcgcctacgg cctgcgcccg gtggtcgagc tgcagttcgc cgactacatg
     1321 tacccagcct acgatcagat cgtctccgag gcggcgcggc tacgctaccg ctcggcgggc
     1381 gacttcacgg cgccgctgac gatacgcatg ccctgcggcg gcggcatcta cggcgggcag
     1441 acccacagcc agagcccgga ggggctgttc acccatgtct gcggcctgcg caccgtgatg
     1501 cccagcaacc cctatgacgc caagggcctg ctgatcgcgt ccatcgagaa cgacgacccg
     1561 gtgatctttc tggagcccaa gcgcctctac aacgggcctt tcgacggcca ccacgacaag
     1621 ccggtcgtgc cctggtccaa gcatgcgctg ggcgaagtgc ccgaggggca ttacacggtg
     1681 ccgctcgaca cggccaagat cttccgccct ggcaaggatc tcaccgtgct ggcctacggc
     1741 accatggtct tcgtctgcga ggcggcggcg attgagagcg gcatcgacgc cgagatcatc
     1801 gacctgcgca gcctgtggcc gctggacctc gacaccatcg tcgcgtcggt gaagaagacc
     1861 gggcgctgcg tcgtcgtgca cgaggccacg cgcaccagcg gcttcggcgc cgagctggct
     1921 tcgctggtgc aggagcattg cttctatcac ctcgaagcgc cgatcgagcg cgtgaccggc
     1981 tgggacacgc cttatccgca tgcgctggag tgggcctact tccccgggcc tgcaagggtc
     2041 gctgcggcct acaagcgcgc gatggaggac tagatgaacg aaccgcttcg ccgatccccg
     2101 gcaggagatg cctgatgggc atccacgtga tcaagatgcc ggacatcggc gagggcatcg
     2161 ccgaggtcga gttggtggcc tggcatgtca agcccggcga ccgcgtcgtc gaggaccagg
     2221 tgctggccga tgtgatgacc gaaaaggcca gcgtcgagat cccttcgccg gtggcgggcg
     2281 aggtgatggc cctgggcgga gagatcggcc aggtgttggc ggtaggttcg gagctgatcc
     2341 gcatcgaagt cgaaggcgcg ggcaacgtga aggcggcggc aagtgcagcc gcagccgctt
     2401 cagccaaggc cctagcgccc gcgccagctg cgaagccggc cgccgccgcc aacaagcccg
     2461 cgtcgccgac tgcactcgtt ccggtaccgt cgctgcccgc gcacgaaccc gcgcggccgc
     2521 tgtatgccga tcgacccatc gcctcgccct ccgtgcgccg ccgcgcctgg gagctgggcg
     2581 tcgaccttgc gcaggtgccg gccagcggca cggccgggcg catcatgcag gccgacctcg
     2641 acgcctacgc tgcgcgcgac agcggcgcgc gcccggcgac ccggcccacg gcctcgctta
     2701 tcccactcgc tccgcgcggc gtggcgcggc gcgacgaaga agcgatcaag ctgatcggcc
     2761 tgcgccgcaa gatcgcgcag aagatgcagg aggccaagcg gcgcatcccg catttcagct
     2821 atgtcgaaga ggtcgacgtc accgagctcg aggcgctgcg cgccaggctc aatgccaaat
     2881 ggggtgcgca gcggccgcgc ctgaccgtgc tgcccttcct ggtccgcgcg ctggtgctga
     2941 gcttgcgcga gcatcccgag atcaatgccc gct
//
LOCUS       DASUCY010000693         2531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_674555, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000693 DASUCY010000000
VERSION     DASUCY010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674555"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1456)
                     /locus_tag="VFV25_13680"
     CDS             complement(<1..1456)
                     /locus_tag="VFV25_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
                     /protein_id="HEY1394418.1"
                     /translation="MLGMSKGNGADARPGRSTRVGPRPRHWTQALQENPLARFIPFSS
                     LVSPHDVITRGGDYLRVWRLDGVPFECADEHQIAERHEALCSLLRNLAGGQWAVWTHR
                     LHRVVADRLQDPVQPGFARDLSRAYQAKLSERRMMSNELYLTLVYRPNVSRVSRALQS
                     TQRTRAAIAEAQAEALRIMEERSALVGRVLRGFGPQLLGTREQRGRTYSEVAEFLGYL
                     VNGCWRPIPMTAGPLYRTLPTARLSFGGDKLELRQGNQRRYAALVDIKEYADAVEPGI
                     LNALLYEASEFIETQSFSILPRREAMRALELQRDQLIASDDVVASQVAEMDVALNELG
                     DGQFCMGEYHYSLVVFGAEGEDSVADAGRRAAQAIGAVGEASSLQMAPVDLVADAAWF
                     AQMPGNWQWRPRDAKLSSRAFAALASGHNFARGKRDGNPWGEALALLRTPSGQPFYLN
                     LHSSPEGEDSADKKLPGNTLIIGSTGVGKTTLEMF"
     gene            complement(1440..1772)
                     /locus_tag="VFV25_13685"
     CDS             complement(1440..1772)
                     /locus_tag="VFV25_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HEY1394419.1"
                     /translation="MQGEAIYKGATRPAMKLGIPLVPLVVLFGTGMLLIMWGGILVSW
                     WIALGVVVAFVPALLWMRFVTAKDDQRFRQMFVAMKLRLHDRNRRFWQARSYAPNLYR
                     GARDAWHV"
     gene            complement(1789..2079)
                     /locus_tag="VFV25_13690"
     CDS             complement(1789..2079)
                     /locus_tag="VFV25_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HEY1394420.1"
                     /translation="MRSLESIRRTGPTLAAALLTLLSRAALAQGFDKINTTVTNVNTI
                     LVTISVAVVTIAIIWAGFKMIFQGARLADVANVLIGGTLVGGAAAFASYIVN"
     gene            complement(2076..>2531)
                     /locus_tag="VFV25_13695"
     CDS             complement(2076..>2531)
                     /locus_tag="VFV25_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEY1394421.1"
                     /translation="ADTARALVSVESAFNPWAIGVVGGVLVRQPRHQAEALATARALL
                     AAGWNFSVGLGQINVGNFQRLGLTLDSAFEPCTNLMAMQTVLSECFDRARASAATPTP
                     NQSALRQALSCYYSGNFSTGFRHGYVRKVVVAAGTAARPALTTQLKEKV"
BASE COUNT          395 a          829 c          894 g          413 t
ORIGIN      
        1 ggaacatctc cagcgtcgtc ttgccgacac ccgtcgagcc gatgatcagc gtattgcccg
       61 gcagtttctt gtcggccgag tcttcgcctt cggggctgct gtgcaggttc agatagaagg
      121 gctggcccga tggcgtgcgc agtagcgcca gggcctcgcc ccacgggttg ccgtcgcgct
      181 tgccacgcgc gaagttgtgg ccgctagcca gggccgcgaa ggcacgcgag gacagcttgg
      241 catcgcgagg gcgccactgc cagttgcccg gcatctgggc gaaccaggcg gcatcggcca
      301 cgaggtcgac cggggccatc tgcaggctgg aggcttcgcc gacggcgccg atggcctgcg
      361 ccgcgcgtcg gccggcgtcg gccacgctgt cttcaccctc cgcgccgaag acgaccaggc
      421 tgtagtggta ctcgcccatg cagaactggc cgtcgccgag ttcattgagc gccacatcca
      481 tctcggccac ctggcttgcc accacgtcgt cgctggcgat cagctggtcg cgctgcagtt
      541 cgagggcgcg catggcttcg cgcctgggca ggatcgagaa gctctgggtc tcgatgaact
      601 cgctcgcttc gtacaacagt gcgttcagga tgccgggctc gacggcatcg gcgtactcct
      661 tgatgtcgac cagggcggca tagcgacgct gattgccctg ccgcagctcg agcttgtcgc
      721 caccgaagga cagtcgtgcg gtcggtaggg tgcgatacag cggtccggcc gtcatcggga
      781 tcggtcgcca gcaaccgttg accaggtagc cgaggaactc ggccacttcg gagtacgtcc
      841 ggccgcgttg ttcccgcgtg ccgagcagtt gaggcccgaa accgcgcagc acgcggccga
      901 ccagggccga gcgctcttcc atgatgcgca gcgcctcggc ctgtgcttcg gcgatggcgg
      961 cgcgcgtgcg ctgcgtcgat tgcagcgcgc ggctgacgcg ggacacgttg ggccggtaga
     1021 ccagcgtcag gtagagctcg ttgctcatca tccggcgctc gctgagcttg gcctggtagg
     1081 cgcgcgacag gtcgcgggcg aagccgggct gcacaggatc ctggaggcgg tcggcaacta
     1141 cgcggtgcag ccggtgcgtc cacaccgccc actggccgcc ggccaggttg cgcagcaggc
     1201 tgcacagggc ctcgtgccgt tcggcgatct ggtgctcgtc ggcgcactcg aagggcacgc
     1261 cgtcgaggcg ccacacgcgc aggtagtcgc cgccgcgtgt gatcacatcg tgcgggctga
     1321 ccagcgacga gaagggaatg aaccgggcca gcgggttctc ctgcagtgcc tgggtccagt
     1381 gccgcgggcg cgggcccacg cgggtgctgc ggccgggtct ggcgtcggcg ccgttaccct
     1441 tagacatgcc aagcatcgcg ggctccgcgg tagagattgg gtgcatagct gcgcgcctgc
     1501 cagaagcggc gattgcggtc gtgcaggcgc agcttcatcg cgacgaacat ctgacggaaa
     1561 cgctggtcgt ccttggcggt cacgaagcgc atccacagca gcgccggaac gaaggccacc
     1621 acgacgccga gagcgatcca ccagctcacc aggatgccgc cccacatgat cagcagcatg
     1681 ccggtgccga acagcaccac cagcggcacc agcgggatgc ccagcttcat cgccgggcgc
     1741 gtggcgccct tgtagatggc ttcgccttgc atgtggcgtc cgtgtcggtc agttgacgat
     1801 gtagctggcg aaggctgcgg cgccgccgac gagggtgccg ccgatcagca cattggccac
     1861 gtcggccagg cgcgcgccct ggaagatcat cttgaagccc gcccagatga tggcgatcgt
     1921 caccaccgcc accgagatcg tcaccaggat ggtgttgacg ttggtgaccg tggtgttgat
     1981 cttgtcgaaa ccctgcgcca gggccgcccg cgacagcaac gtcagcagcg cggcggccag
     2041 tgtcggcccg gtgcggcgga tcgattcgag ggatctcata ccttctcctt cagttgggtc
     2101 gtgagggcag ggcgtgccgc ggtacctgcg gcgacgacaa ccttgcgcac gtagccgtga
     2161 cggaagccgg tgctgaagtt gccgctgtag tagcaggaca gggcttgacg cagtgccgat
     2221 tggttcggtg tcggcgtcgc ggccgatgcg cgggcccggt cgaagcactc ggacagcacg
     2281 gtctgcatcg ccatcaggtt cgtgcagggc tcgaaggccg agtcgagcgt caggcccagg
     2341 cgctggaagt tgccgacgtt gatctgcccc aggccgacac tgaagttcca gccggcggct
     2401 agcagggccc gggccgtggc caaggcttct gcctggtggc gcggctggcg caccagcaca
     2461 ccgccgacga cgccgatcgc ccaggggttg aaggcactct cgacactgac cagggcgcgt
     2521 gccgtgtcgg c
//
LOCUS       DASUCY010000694         3045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_658605, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000694 DASUCY010000000
VERSION     DASUCY010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658605"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="VFV25_13700"
     CDS             <1..912
                     /locus_tag="VFV25_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEY1394422.1"
                     /translation="FFFGGHKFRDDKEGGHGSVDMYKSIVQSCDTYYYMLANDMGVDL
                     IHDELQPFGFGELTGIDIDGEVRGLLPSTDWKRRAYRKPEQQRWYAGETISLGIGQGY
                     NSFTVLQLAQATATLANGGVHYRPRLVREIEDVVTHQTRSISMETMATVPVKPENLAV
                     VRKAMIGVNIEGTSATAFRGASYVAAGKTGTAQVVGIKQNEKYNAAKVAEQLRDHALY
                     IAFAPADAPRVVVALVVENAGFGAQSAAPIARRVFDYVLSGQYPAEEDIAAVQAGQAT
                     APVGTQRKAEAVPNPQAASAPEPGDQP"
     gene            1022..2176
                     /gene="rodA"
                     /locus_tag="VFV25_13705"
     CDS             1022..2176
                     /gene="rodA"
                     /locus_tag="VFV25_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEY1394423.1"
                     /translation="MRTSFEKPSVWQRVLPLFLGFDGVLGLVVVLLACAGMLTMYSAG
                     YDAGTRFVDHCRNMALAAAILFVVAQIPPQRLMQLAVPLYTVGVALLVATALFGITKK
                     GAQRWLNVGVVIQPSEVLKIAMPLMLAWWFQRREGQLRGLDFVVAAGLLLVPVGLIIK
                     QPDLGTAILVLSAGLYVIFFAGLSWRLILPVLVLGMVCVIAIVASENTICQPDLQWPL
                     LREYQKHRICTLLDPSQDPLGKGFHIIQGMIAIGSGGVPGKGFMNGTQTHLEFIPERT
                     TDFIFAAFSEEFGLIGCVLLLLGFTALILRGLMIAADAPTQFSRLLAGAVTLSFFTYC
                     FVNMGMVSGILPVVGVPLPFISYGGTAMVTLGLGLGILMSIAKSRRLMQS"
     gene            2185..2595
                     /locus_tag="VFV25_13710"
     CDS             2185..2595
                     /locus_tag="VFV25_13710"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEY1394424.1"
                     /translation="MSQLVAINAAASINSEDDLRDLEHARLRALVEGNIALARQLHAD
                     DFHLVTPIGRSYSKDRYLSEIENGALRYHSWVPGAMQVRSFGSTAVLRYQATLELDSG
                     GGRTPPFRCWHIDSYELINGLWQVVWSQATSIRP"
     gene            complement(2620..>3045)
                     /locus_tag="VFV25_13715"
     CDS             complement(2620..>3045)
                     /locus_tag="VFV25_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEY1394425.1"
                     /translation="TSAALLAATCAPWLLRLFTHDPAIIEAATKLLWITVLLEPGRTF
                     NLVVINALRATGDARFPVAAGAVSMALVLAGGSWLLGEHLGLGLTGVWIAYAADEWIR
                     GLLMWWRWEGHGWVRHARATHRRLRAARPGGAVGVDSVL"
BASE COUNT          485 a         1048 c         1019 g          493 t
ORIGIN      
        1 ttcttcttcg gcggccacaa gttccgcgac gacaaggaag gcggccacgg cagcgtggac
       61 atgtacaagt ccatcgtgca gtcctgcgac acctactact acatgctggc caacgacatg
      121 ggcgtggacc tgatccacga cgagctgcag cctttcggct tcggcgagct caccggcatc
      181 gacatcgacg gcgaggtgcg cggcctgctg ccgtcgaccg actggaagcg ccgcgcctac
      241 cgcaagccgg agcagcagag atggtatgcc ggcgagacca tctcgctggg catcggccag
      301 ggctacaaca gcttcaccgt gctgcagctg gcccaggcca ccgcgacgct ggccaacggc
      361 ggcgtgcact accggccgcg cctggtgcgc gaaatcgagg atgtggtgac gcaccagacc
      421 cgcagcatct cgatggagac catggccacg gtgccggtca agcccgagaa cctggcggtg
      481 gtgcgcaagg ccatgatcgg cgtcaacatc gaaggcacgt cggccacggc ctttcgcggt
      541 gcttcctatg tggcggccgg caagaccggc acggcccagg tggtgggcat caagcagaac
      601 gagaaataca acgccgccaa ggtggccgag cagctgcgcg accacgccct ctacatcgcc
      661 ttcgcgccgg ccgacgcgcc gcgcgtggtg gtggccctgg tggtggaaaa cgccggcttc
      721 ggcgcgcagt cggcggcgcc gatcgcgcgc cgcgtcttcg actacgtgct gagcgggcaa
      781 taccctgccg aggaagacat cgccgccgtg caggcgggcc aggcgacggc tccggtgggc
      841 acgcagcgca aggcggaagc cgtgcccaac ccgcaggcgg cttccgcgcc cgagccggga
      901 gaccagccat gagacatcca gtgccaacct gtccttcgcg ggtcgagcgc cgggccgctc
      961 ccaagccggc ccgcatcccc tcgggggatc ggacgacgta cgcgtcggac gaggggttgt
     1021 catgaggacg agcttcgaga agccttcggt atggcagcgc gtgctgcccc tcttcctcgg
     1081 cttcgacggg gtgctgggcc tggtggtggt gctgctggcc tgcgccggca tgctgaccat
     1141 gtattcggcc ggctacgacg ctggcacccg cttcgtcgac cattgccgca acatggcgct
     1201 ggccgccgcc atcctgttcg tggtggcgca gatcccgccg cagcgcctga tgcagctggc
     1261 cgtgccgctc tataccgtcg gcgtcgcgct gctggtggcg accgccctgt tcggcatcac
     1321 caagaagggg gcgcagcgct ggctcaacgt gggcgtcgtg atccagccca gcgaggtgct
     1381 gaagatcgcg atgcccttga tgctggcctg gtggttccag cgccgcgagg ggcaactgcg
     1441 cgggctggac ttcgtggtcg ccgccggatt gctgctggtg ccggtgggct tgatcatcaa
     1501 gcagcccgac ctgggcaccg cgatcctcgt gctttcggcc gggctttatg tgatcttctt
     1561 cgccggcctg agctggaggc tgatcctgcc ggtgctggtg ctgggcatgg tctgcgtgat
     1621 cgccatcgtc gccagcgaga acaccatctg ccagcccgac ctgcaatggc cgctgctgcg
     1681 cgagtaccag aagcaccgca tctgcaccct gctcgacccg agccaggacc cgctgggcaa
     1741 gggcttccac atcatccagg gcatgatcgc gatcggctcc ggcggcgtgc caggcaaggg
     1801 cttcatgaac ggcacccaga cccatctgga gttcatcccc gagcgcacca ccgacttcat
     1861 cttcgccgcc ttctccgagg agtttggcct gatcggctgt gtgctgctgc tgctcggttt
     1921 cacggccctg atcctgcgcg ggctgatgat cgcggccgat gcgccgacgc agttctcgcg
     1981 cctgctcgcc ggggccgtga cgctgagctt cttcacctac tgcttcgtca acatgggcat
     2041 ggtcagcggc atcctgccgg tggtgggcgt gccgctgcct ttcatcagct acggcggcac
     2101 ggcgatggtg acgctgggcc tgggcctggg catcctgatg tcgatcgcga agagccgacg
     2161 gctgatgcag agttagacgc tgcgatgtcc cagctcgtag ccatcaacgc ggccgcatcc
     2221 atcaactccg aggacgacct gcgcgacctc gagcatgctc ggctgcgcgc ccttgtcgag
     2281 gggaacatcg cgctcgcccg gcagcttcat gccgatgact tccacctcgt gacgccgatt
     2341 ggacgctcgt actcaaaaga ccggtacctg agcgagatcg aaaacggcgc gctgcgctac
     2401 cactcgtggg tgcctggagc catgcaggtg cgcagcttcg gtagcactgc ggtcctacgc
     2461 tatcaagcga cgctcgagct cgattcgggc ggcggacgca ccccgccatt tcggtgctgg
     2521 cacatcgact cgtacgaact catcaatggg ctctggcaag tcgtctggtc gcaggcaacg
     2581 agcattcggc cgtgagggcc ccacgcccct gccgccgcct caaagcaccg aatcgacccc
     2641 caccgccccg cctggcctcg cggcgcgcag ccgccggtgc gtggcgcgcg cgtggcgcac
     2701 ccagccgtgg ccttcccagc gccaccacat caaaagcccg cggatccact cgtcggccgc
     2761 gtaggcgatc cacacgccgg tcaggccgag cccgaggtgc tcgcccagca gccagctgcc
     2821 gccggccagc accagggcca tcgatactgc gccggcggcc accgggaagc gtgcgtcgcc
     2881 ggtggcgcgc agcgcattga tgaccacgag gttgaaggtg cggcccggct ccagcagcac
     2941 ggtgatccac agcagcttcg tggcggcctc gatgatggcc ggatcgtgcg tgaagaggcg
     3001 cagcagccag ggcgcgcagg tggcggccag cagtgccgcc gaggt
//
LOCUS       DASUCY010000695         2635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1012034, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000695 DASUCY010000000
VERSION     DASUCY010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012034"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1459
                     /locus_tag="VFV25_13720"
     CDS             <1..1459
                     /locus_tag="VFV25_13720"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEY1394426.1"
                     /translation="LDANGHARPQLHDLPASQLRQGENTLYLRMHIARRAGLVAPQVG
                     DAATLRTAYERKLFIAFTGPRVLFGATLTLGLFMLVLWQRRPAEASFGYFGLAATSFA
                     LMLFDLLLDATPLEPASWDCVRNLAAQLVNVSTFVFALRYGGWRLPRFEAALWSLVPI
                     QLVFNVLSVAGIALPMSVRWLAAPIFMGYVAVFAWIAWRRRTAESIALLLASPGRAIW
                     FLLHYGGLGPYDLSELLLPYSFVPLFVVIGWMLVSRFARSLNESEQLNAQLEQRVEQK
                     RIEMQQNFQRLQRLEREQAIAEERSRIMSDMHDGIGAHLISMLDLAERGALSSDEMTA
                     ALRDCLDDLRLTIDSLEPSDNDLLPVLGNFRYRVDPRLRKQGIALDWQVSEVPRLACL
                     TPRNLLHLLRILQEAFTNVLKHAHASVISVQTGLDASGRSVFIRVRDNGGGFKGEHRG
                     HGLDNMRQRAQSIGAVLAIDGSAGGTTLELLLPVG"
     gene            1513..2508
                     /locus_tag="VFV25_13725"
     CDS             1513..2508
                     /locus_tag="VFV25_13725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEY1394427.1"
                     /translation="MSPFKAYLIEQAGDGKAAGRMTTLSPEQLDAGEVTLRVHYSSIN
                     YKDALAATGAGKIIRRFPCVGGIDMAGVVVDSADARFKPGDPVIATSFDIGVSHHGGY
                     AELARVPARWVLPLPQGLDLFESMAIGTAGFTAALGIVRMEDNGLAPDKGPVIVTGAS
                     GGVGALAIDMLAQLGYHTVALTGKESESDFLRGLGAAEIRLRSHIDFDKVRPMEAATW
                     AGAIDNVGGPILHWLLATMKQAGTVASIGNAASFKLDTTVFPFILRGVSLLGIDSGYM
                     GFPTRQRVWDRLASDLKPRHLAAFTKQIAFGQLPAAFDDYIQGRAKGRTVVRIGG"
BASE COUNT          407 a          875 c          891 g          462 t
ORIGIN      
        1 gctggatgcg aacgggcacg cccgaccgca gcttcatgat ttgccggcct cgcaattgcg
       61 tcaaggcgaa aacaccctct atctgcgtat gcacatcgca aggcgggcgg ggttggtcgc
      121 tccgcaggtc ggcgacgcgg cgacgctgcg caccgcctat gagcgcaagc ttttcatcgc
      181 ctttaccggg ccccgcgtgc tgttcggagc gaccctgacg ctcggcctgt tcatgctggt
      241 gctgtggcag cggcgtccgg ccgaggccag cttcggctac ttcggcctgg cggcgacctc
      301 gttcgccttg atgctgttcg acctgctgct cgacgcaacg cccctggagc ccgccagctg
      361 ggactgcgtg cgtaatctgg cggcgcagct ggtcaacgtg tcgaccttcg tcttcgcact
      421 gcgctacggc ggctggcgcc tgccgcgctt cgaggccgcg ctgtggtcct tggtgccgat
      481 acagctcgtg ttcaacgtgc tgagtgtggc cggcatcgcg ctgccgatga gcgtcaggtg
      541 gctggcggcc cccatcttca tgggctatgt cgcagtgttc gcctggatcg cctggcgcag
      601 acgcacggct gaaagcatcg cgctgctgct ggcctcaccg ggccgcgcca tctggttcct
      661 gctgcactac ggcgggctcg gaccctacga tctgtccgag ctgctgctgc cttactcctt
      721 cgtcccgctg ttcgtggtga tcggctggat gctcgtgagc cgcttcgcgc gatcgctgaa
      781 cgaatcggag cagctcaatg cgcagctcga gcagcgtgtc gagcagaagc ggatcgaaat
      841 gcagcagaac ttccagcgcc tgcaacgcct cgagcgggag caggcgatcg ccgaggaacg
      901 cagccgcatc atgagcgaca tgcacgacgg catcggcgcg cacctcatct ccatgctgga
      961 tctggccgag cgcggcgctc tctcgagcga cgagatgacg gccgcgctgc gtgattgcct
     1021 ggacgatctg cgcttgacca tcgattcgct cgaacccagc gacaacgacc tgctgcccgt
     1081 gctcggcaac ttccgctacc gtgtcgaccc gcggctgcgc aagcagggca tcgcgctcga
     1141 ctggcaggtg agcgaggtgc cgcggctggc gtgtctcacg ccgcgcaacc tgctgcatct
     1201 gctgcgcatc cttcaggagg ccttcaccaa tgtgctcaag catgcgcacg ccagcgtgat
     1261 cagcgtgcag accggactcg atgccagcgg gcgctcggtg ttcatccgcg tgagggacaa
     1321 cggaggaggg ttcaagggcg agcaccgcgg gcacggattg gacaatatgc gtcagcgcgc
     1381 gcagagcatc ggcgcagtgc tggccatcga tggctcggca ggcgggacga cgctggagct
     1441 gttgctgccg gtcggctagg atctgtgcgc ccggaccata cccactcccg cccggcttgc
     1501 aaggagaccg ctatgagccc cttcaaggcc tatctgatcg agcaggccgg cgacggcaag
     1561 gccgccggcc gcatgaccac gctttcaccc gagcagctcg atgccggcga ggtgacgctg
     1621 cgggtgcact actcgagcat caactacaag gatgcgctgg ccgccacggg tgccggcaag
     1681 atcatccggc gctttccttg cgtgggcggc atcgacatgg ccggcgtcgt ggtggacagc
     1741 gccgatgcgc gcttcaagcc tggcgatccg gtgatcgcga ccagcttcga catcggcgtc
     1801 tcgcaccacg gaggctatgc cgagctggcg cgcgtgccgg cgcgctgggt gctgccgctg
     1861 ccccaggggc tggatctgtt cgagtccatg gccatcggca cggccggctt cacggcggcc
     1921 ttgggcatcg tgcgcatgga agacaacggc ctggcgcccg acaaggggcc ggtgatcgtg
     1981 acaggggcct cgggcggcgt cggcgcgctg gcgatcgaca tgctggcgca gctgggctat
     2041 cacacggtcg cattgaccgg caaggaaagc gagagcgatt tcctgcgcgg cctgggcgcg
     2101 gccgagatca ggctgcgctc gcacatcgac ttcgacaagg tccgccccat ggaggcggcg
     2161 acctgggccg gcgccatcga caacgtcggc gggcccatcc tgcactggct gctcgcgacc
     2221 atgaagcagg ccggcaccgt ggccagcatc ggcaatgcgg ctagcttcaa gctggacacg
     2281 acggtcttcc ccttcatcct gcgcggggtc agcctgctgg gcatcgattc gggctacatg
     2341 ggtttcccga cgcggcagcg cgtgtgggac cgcctggcct cggacctcaa gccgcgccac
     2401 ctcgcggctt tcacaaaaca gatcgctttc ggccaactgc ccgcggcctt cgacgactac
     2461 atccaggggc gcgccaaggg gcgcactgtg gtgcgcatag gtgggtgagc gccgccgtcg
     2521 cgtcgtctac ttcttgacca gcgggcaggt cgagtccgac agcgggccga aggcctgctc
     2581 gccgggaagc gtctttacga gcttgtagta gtcccagggg tacttggact cctcg
//
LOCUS       DASUCY010000696         3506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1012035, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000696 DASUCY010000000
VERSION     DASUCY010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012035"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="VFV25_13730"
     CDS             <1..478
                     /locus_tag="VFV25_13730"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEY1394428.1"
                     /translation="LPDSEAARLGRAVHRVLEWAVRRPPGRRAAELDACVAAVGAEFR
                     LQAAQKGSVRALSEAVLAGAEVRRFLDPRQSAWSGNEVPLSWRGEQIRLDRLVAFDTE
                     TGREWWVLDYKLQHAPEQLAGYRAQLENYRRAVQAAQPGERVRAAFITGAGRLVEL"
     gene            complement(650..748)
                     /gene="ffs"
                     /locus_tag="VFV25_13735"
     ncRNA           complement(650..748)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFV25_13735"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(795..1913)
                     /locus_tag="VFV25_13740"
     CDS             complement(795..1913)
                     /locus_tag="VFV25_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEY1394429.1"
                     /translation="MSSKPSTDTLSYEQAGVNYDLIDPLKIAAQRAAKDTAKNLATHG
                     FDEVVASRGESAYVVDVGPFYLATIVECLGSKALVADEMAKLTGRSFYDGIAQDTIAM
                     AINDLITVGATPLVVQAYWAAGGSDWFGDAQRAQALVAGWKRACDTCGVAWGGGETPA
                     LAGIVEAGRIDLAASCTGIINPKERLSVGDKLGAGDAIVLLASSGIHANGLSLARKLV
                     ERLPQGYLTPVTSELNYGEALLAPTVLYSPVTEALHKAGITPHYCANITGHGWRKLLR
                     HPAGFTYRIRTVPEVTPVLRFIQQHTNQDDREAYSTLNMGAGFALFVKAEHAQRTVEI
                     AQAQGIPALVAGAVEAGPKKLIVEPLGISFGDEDLQLR"
     gene            2005..>3506
                     /gene="dnaX"
                     /locus_tag="VFV25_13745"
     CDS             2005..>3506
                     /gene="dnaX"
                     /locus_tag="VFV25_13745"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEY1394430.1"
                     /translation="MSYTVLARKYRPLDFEQMVGQEHVVQALSNALTQQRLHHAYLFT
                     GTRGVGKTTVSRILAKSLNCTGPDGMGGITAHPCGQCAACRDIDSGRFVDYVELDAAS
                     NRGVEEISQLLDQAVYKPVVGRFKVYMIDEVHMLSTTAFNAMLKTLEEPPEYLKFVLA
                     TTDPQKVPVTVLSRCLQFNLRPMATEVVQEHLQHVLQAEAIPAEPGALRLLARAARGS
                     MRDALSLTDQAIAFGAGQLQEEAVRQMLGAVDRGHVVRIVQALAASDGAGLLAVVDAL
                     RGLGLSAAGTLEELAATAQQMAVLQAVPQALDPNDPEAQTQQLLAGLLAPDETQLLYS
                     IAVHGRAELALAPDEYSGLSMVLLRMLAFRPQGAVVNAPQRPVAAPARIAVEGAAGSA
                     AAARSAALRAPAPASRSSAASAAAKDTEIPPWIDAPMATEIMAAEEVPRSVRASSSSA
                     AIAAVPLDAEPETLSSADIALGDRWSALVRSLCEAGAIAALVRELAMQAQ"
BASE COUNT          515 a         1179 c         1239 g          573 t
ORIGIN      
        1 cctgcccgac agcgaggcgg cgaggctggg ccgcgccgtt catcgcgtgc tcgaatgggc
       61 agtgcgccgg ccgccggggc ggcgcgccgc ggagctcgat gcctgcgtcg ctgcggtggg
      121 cgcggagttc cgcctgcaag ccgcgcaaaa gggcagcgtc agggcgctca gtgaagcggt
      181 gctggccggc gccgaggttc ggcgtttcct cgatccccgg caatcggcct ggtcgggcaa
      241 cgaggtgccg ctgtcctggc ggggcgagca gatccgcctg gatcgcctgg ttgccttcga
      301 taccgagacg gggcgggagt ggtgggtgct ggactacaag ctgcagcacg cacccgagca
      361 actggccggc tatcgcgcgc agctggagaa ctaccgccgc gccgtgcagg ccgcgcagcc
      421 gggcgagcgc gtgcgggctg ccttcatcac cggcgctggg cgcttggtgg agctgtagcc
      481 tgctgcccag ctcttcttca tagccacgac gcagaccgat gaatcccatc cttcactaca
      541 tccacgaccc gctgtgcggc tggtgctacg ccgccgatcg ccgcggctgc cgatgcggcc
      601 tcgcagggcc aggcaggtcc tgggtgcgat acggatggtt gtgtggtggg gtgacgagcc
      661 tttcccccgg cactggaggc taacggttag ggctgcttcg ttcccgacct gacccgtttg
      721 gccgcgcccc catgcgggga ggcccgtcac ttcggattgt aggctagggg cagaggaccc
      781 ttcggccgtc gcggtcagcg cagctgcaga tcttcgtcgc cgaagctgat gcccagcggc
      841 tcgacgatca gcttcttggg tccggcctcg acggcgccgg ccaccagcgc cgggatgccc
      901 tgggcctgcg cgatctcgac ggtgcgctgc gcatgctcgg ccttcacgaa gagcgcgaag
      961 ccggcgccca tgttgagcgt gctgtaggcc tcgcggtcat cctggttggt gtgctgctgg
     1021 atgaagcgca gcaccggcgt gacctcgggc acagtgcgga tgcggtaggt gaagccggcc
     1081 gggtggcgca gcagcttgcg ccagccgtgg ccggtgatgt tggcgcagta gtgcggcgtg
     1141 atgccggcct tgtggagcgc ctcggtgacc ggtgaataca gcaccgtcgg cgcaagcagc
     1201 gcctcgccgt agttcagctc ggaggtcacg ggcgtcagat aaccctgtgg cagccgttcg
     1261 accagcttgc gtgccaggct caggccgttg gcatggatgc cgctggaggc gagcagcacg
     1321 atggcgtcgc ccgcgccgag cttgtcaccc accgacagcc gttccttggg attgatgatg
     1381 ccggtgcagg aggccgcgag gtcgatgcgg cccgcttcca cgatgccggc cagcgctggc
     1441 gtctcgccgc cgccccaggc cacgccgcag gtgtcgcagg cgcgcttcca gccggccacg
     1501 agggcctgcg cgcgctgcgc atcgccgaac cagtccgagc cgccggcggc ccagtaggcc
     1561 tgcaccacca gcggcgtggc gcccacggtg atgaggtcgt tgatcgccat cgcgatggtg
     1621 tcctgcgcga tgccgtcgta gaaactgcgg ccggtaagct tggccatctc gtcggcgacc
     1681 agggccttgg agcccaggca ttcgacgatg gtggcgagat agaacggtcc cacgtccacc
     1741 acataggccg attcgccgcg cgaggccacc acttcgtcga agccgtgcgt tgcgaggttc
     1801 ttcgccgtgt ccttcgcggc gcgctgggcc gcgatcttca ggggatcgat caggtcgtag
     1861 ttgacccccg cctgttcgta gctgagggtg tcggtggaag gcttggagct catggaatcg
     1921 cgggtggctg cggagaaagt ggaattatcc ggcaggggcg gacctggccg aagcgctgcc
     1981 gatgcgaccc gtagaatgcc cctcatgagc tacaccgtcc tagcccgcaa ataccgcccg
     2041 ctcgatttcg agcagatggt gggccaggag cacgtggtgc aggcgctttc caatgcgctg
     2101 acgcagcaac ggctgcacca cgcctatctc ttcaccggca cgcgcggcgt gggcaagacc
     2161 acggtgtcgc gcatcctcgc caagtcgctc aactgcacgg gccccgacgg catgggcggc
     2221 atcactgctc atccttgcgg ccaatgcgcg gcctgccgcg acatcgacag cggccgcttc
     2281 gtcgactatg tggagctcga tgccgcgtcc aaccgcgggg tcgaggagat ctctcagttg
     2341 ctggaccagg cggtctacaa gccggtggtt gggcgcttca aggtctacat gatcgacgaa
     2401 gtgcacatgc tctccaccac cgcgttcaac gcgatgctga agacgctgga ggagccgccg
     2461 gaatacctca agttcgtgct ggccacgacc gaccctcaga aggtgccggt cacggtgttg
     2521 tcgcgctgcc tgcaattcaa tctgcggccg atggcgacgg aggtcgtgca ggaacatctg
     2581 cagcacgtgc tgcaggccga ggcgataccg gccgagccgg gcgcattgcg cctgctggcg
     2641 cgggccgcgc gcggctcgat gcgcgatgca ctctcgctca ccgaccaggc catcgccttc
     2701 ggtgccgggc aactgcagga agaagcggtg cgccagatgc tcggcgccgt cgaccgcggc
     2761 catgtcgtgc gcattgtcca ggcgctggcc gcctccgatg gcgcaggcct gctcgccgtc
     2821 gtggacgcct tgcgcggcct ggggctgtcg gccgcaggca cgctcgaaga actcgccgcg
     2881 acggcccagc agatggcggt gctgcaggcc gtgccccagg cgctcgaccc caacgatccc
     2941 gaggcccaga cccagcagtt gctcgccggc ctgctggcgc ccgacgagac ccagctgctc
     3001 tacagcattg ccgtgcatgg ccgggccgag ctggcgctgg cgcccgacga atacagcggc
     3061 ctgtccatgg tgctgctgcg catgctggcc tttcgtcccc agggcgccgt tgtgaacgcg
     3121 ccgcagcggc cggttgcggc cccggcgcgc attgccgttg aaggagcggc aggatcggcc
     3181 gccgcagctc gcagcgctgc cctgcgtgcg ccggcccccg cgagccgcag cagtgccgca
     3241 agcgctgccg ccaaggacac tgagatcccg ccctggatcg atgctcccat ggcgacggag
     3301 atcatggcag cagaggaggt gccccggtct gtgcgtgcct cttcatcgag cgcggcgatt
     3361 gcggccgtac ctttggacgc cgaacccgag accttgagca gcgccgatat cgcactcggc
     3421 gaccgctggt ctgcactcgt gcgctcgctg tgcgaggccg gcgccatcgc ggcgctggtg
     3481 cgcgagctgg cgatgcaagc gcaatg
//
LOCUS       DASUCY010000697         3861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_257019, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000697 DASUCY010000000
VERSION     DASUCY010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257019"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="VFV25_13750"
     CDS             <1..734
                     /locus_tag="VFV25_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEY1394431.1"
                     /translation="KQAEEMYGVPPEIVVGILGVETIYGRQMGGYRALDALATLSLDF
                     PKERKDRSDFFRTELEQYLVLCHGEGVDPLALKGSYAGAMGMPQFMPSSWNKYAVDFD
                     GDGRVDLVNSPADAIGSVAHYLAEFGWQRGLPTHYEVAAPVDTTERAALLAPDILPSF
                     TAAQFAEHGAGLPEAARGYDGLLALVELQNGDAAPGYVAGTTNFYAITRYNWSSYYAM
                     AVIELGQTVGRVVEGLRELRSLEQK"
     gene            873..3170
                     /locus_tag="VFV25_13755"
     CDS             873..3170
                     /locus_tag="VFV25_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HEY1394432.1"
                     /translation="MSQEKIELYPYPAGGWGALRSVARQLMQQGIPAKGAKTMLSANR
                     PDGFDCPGCAWPDRNHASSFEFCENGVKAVAAEATARRATPEFFAQHTVSWLAEQSDF
                     FLEDQGRLTHPMAYDAASDRYLPIGWDQAFALIARHLNALPDPNQAIFYTSGRTSNEA
                     AFLYQLFVREYGTNNFPDSSNLCHEPSGVALRASIGVGKGTVTLADFEQADAIFIFGQ
                     NPGTNHPRMLGELRAAAKRGAHILSFNPLRERGLERFANPQSPLEMATLGATRISSHY
                     YQLKIGGDLAALKGIIKHLIEREDCGEGVLDHTFIAEHTTGFDALAADVRAETWEAIE
                     TESGLSREQLREAAEVYARAERVIACWGMGITQHQHSVATIQMIANLLLLRGNIGRPG
                     AGACPVRGHSNVQGDRTMGIHEKPAPAFLDRLGQVFDFEPPRGPGLNAVEAIAAIRDS
                     RAKVFFAMGGNFAAATPDTAATWQALRRCELTVHVATKFNRSHVVHGQAALVLPCLGR
                     TEVDLQAGGPQGVTVEDSMSMVHLSAGINAPASEQLLSEPAIVACLAVATLGARSKTP
                     WLWLIEDYARIRDKIAAVFDDFQGFNERVRVPGGFRLRNMASERIWATPGRRAVFHAH
                     AVPRDLAIHRARRRELDRLVFTLATVRSHDQYNTTIYGHDDRYRGVYGQRRVLFIHAE
                     DLRDLGMQAGAWVDLKSIAADGEERVVRRFKLVAYNIPRGCLAAYYPETNGLLPLSSV
                     AEGAGTPTSKSIPVLVLPHAPQVQS"
     gene            3167..>3861
                     /gene="fdhD"
                     /locus_tag="VFV25_13760"
     CDS             3167..>3861
                     /gene="fdhD"
                     /locus_tag="VFV25_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007839222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEY1394433.1"
                     /translation="MKTGSREVRRCRVGRNETICTTEFDIVAVERPVALVFNGISHAV
                     MLATPLDLEDFALGFALSEGLLDRAEDCYGIEAHPTADGVEVHLDIASRAFARLKEQR
                     RTLAGATGCGLCGMEQLGMLDLHPQRVTARPALQAIAAPTMLKAAAALPSHQPLNTSN
                     GAMHAAAWVSPQGEIQGVGEDVGRHNALDKLIGRRLRARQAGGQPAGLGDGFVLMSSR
                     ASYELLRKCARLD"
BASE COUNT          642 a         1373 c         1292 g          554 t
ORIGIN      
        1 tcaagcaggc cgaggagatg tacggcgtgc caccggagat cgtcgtcggc atcctcggcg
       61 tggaaacgat ctacggccgc cagatgggcg ggtatcgcgc gctggacgcg cttgccacgc
      121 tgagcctgga cttccccaag gaacgcaagg accgcagcga cttctttcgc accgaactgg
      181 agcagtacct cgtgctgtgc cacggcgagg gcgtcgatcc gctcgcgctc aagggctcct
      241 atgccggcgc catgggcatg ccgcagttca tgcccagctc ctggaacaag tacgcggtcg
      301 acttcgacgg cgacggccgc gtggacttgg tgaacagccc ggccgacgcg atcggctcgg
      361 tcgcgcacta cctggccgaa ttcggctggc agcgcggcct gccgacacac tacgaggtcg
      421 cagctcccgt cgacacgacc gagcgcgccg cgctgctggc gcccgacatc ctgcccagct
      481 tcacggccgc gcaattcgcc gagcatggcg ccggcctgcc ggaagcggcg cgcggctacg
      541 acggcctgct ggcccttgtg gagctgcaaa acggcgacgc cgcccccggc tacgtggccg
      601 gcacgacgaa cttctacgcg atcacgcgct acaactggtc gagctattac gcgatggccg
      661 tgatcgaact gggccagacc gtgggccgcg tcgtcgaagg gctgcgcgaa ctgcggtcgc
      721 tcgaacagaa gtagaagcac agcgcgccca ctccatgccg ctcgatctgc ccgccccctg
      781 ccggaagtcg accctgtgcc ctgccccggg ttaaggtagc gcctttgcgg ccccgctcac
      841 ccgaggaggc gaccgcgaca ggaacgaccc agatgagcca agagaagatc gagctttatc
      901 cctaccccgc cggcggctgg ggcgccttgc gcagcgtggc ccgccagctg atgcagcagg
      961 gcatccctgc caagggcgcg aagacgatgc tgtcggccaa ccggcccgac ggcttcgact
     1021 gcccgggttg cgcctggccc gaccgcaacc acgcatcgag cttcgagttc tgcgagaacg
     1081 gcgtgaaggc cgtggccgcc gaggcgacgg cgcgacgcgc gacgccggaa ttcttcgcgc
     1141 agcacacggt gagctggctg gccgaacaga gcgacttctt cctcgaagac caggggcggc
     1201 tcacccaccc gatggcctat gacgcggcga gcgaccgcta cctgccgatc ggctgggacc
     1261 aggccttcgc cctgatcgcc cgccacctga acgccctgcc cgatccgaac caggccatct
     1321 tctacacctc gggacgcaca agcaacgaag cggcctttct ctaccagctc ttcgtgcgcg
     1381 agtacggcac caacaacttc cccgacagct cgaacctgtg ccacgagccc agcggcgtgg
     1441 ccttgcgggc cagcatcggc gtgggcaagg gcacggtgac gctggcggac ttcgagcagg
     1501 ccgatgcgat cttcatcttc ggccagaacc ccggcaccaa ccacccgcgc atgctgggcg
     1561 agctgcgcgc agcggccaag cggggcgcgc acatcctcag cttcaacccg ctgcgcgaac
     1621 gcggactgga acgcttcgcg aacccgcaga gcccgctgga gatggccacg ctgggcgcca
     1681 cgcgcatcag ctcgcattac taccagctca agatcggcgg cgatcttgcc gcgctcaagg
     1741 gcatcatcaa gcacctgatc gagcgggagg attgcggcga aggcgtgctc gaccacacct
     1801 tcatcgccga gcacacgacg ggcttcgatg ccttggccgc cgacgtgcgc gccgagacct
     1861 gggaagcgat cgaaaccgaa tcgggcctga gccgcgagca gctgcgcgag gccgccgagg
     1921 tctacgcgcg cgccgagcgc gtgatcgcct gctggggcat gggcatcacc cagcaccagc
     1981 attcggtggc gacgatacag atgatcgcca acctgctgct cctgcgcggc aacatcggcc
     2041 gtccgggtgc cggcgcttgc ccggtgcgcg gccacagcaa tgtgcagggc gaccgcacga
     2101 tgggcatcca cgagaagccg gcgccggcct tcctcgatcg cctgggccag gtattcgact
     2161 tcgagccgcc gcgcgggcca ggccttaatg ccgtggaggc gatcgcggcc atccgggaca
     2221 gtcgcgccaa ggtcttcttc gcgatgggcg gcaacttcgc cgcggccacg ccggacacgg
     2281 ccgccacctg gcaggcgctg cggcgctgcg aactcacggt gcacgtggcg accaagttca
     2341 accgcagcca cgtcgtgcat ggccaggcgg cgctggtgct gccctgcctg ggccgcaccg
     2401 aggtcgatct gcaggcgggc ggcccgcaag gcgtgacggt ggaagactcg atgagcatgg
     2461 tgcatctgtc ggccggcatc aatgcgccgg cctccgagca gttgctgtcc gagccggcca
     2521 tcgtggcgtg cctggccgtt gccacgttgg gcgcgcgcag caagacgccg tggctgtggc
     2581 tcatcgaaga ctatgcccgc atccgcgaca agatcgcggc cgtgttcgac gatttccagg
     2641 gcttcaacga acgcgtgcga gtgcccggcg gcttccggct gcgcaacatg gccagcgagc
     2701 gcatctgggc cacgcctggg cgccgggccg tcttccacgc gcatgcggtg ccgcgcgatc
     2761 tcgccatcca tcgcgcgcgc cgccgcgagc tcgaccggct ggtcttcacg ctcgccacgg
     2821 tgcgctcgca cgaccagtac aacaccacga tctacgggca cgacgaccgc taccgcggcg
     2881 tctacggcca gcggcgcgtg ctcttcatcc atgccgaaga cctgcgcgac ctgggtatgc
     2941 aagccggcgc ctgggtcgac ctgaagagca tcgccgcgga tggcgaggag cgcgtggtgc
     3001 gccgcttcaa gctggtcgca tacaacatcc cgcgcggatg cctcgccgcc tactaccccg
     3061 agaccaatgg ccttttgccg ctttcgagcg tggccgaagg cgcggggacg ccgacctcca
     3121 agtccattcc ggtgctggta ctgccgcacg ccccgcaggt gcaatcatga agacgggctc
     3181 gcgcgaggtg cgccgctgcc gtgtgggcag gaacgagacc atctgcacga cggagttcga
     3241 catcgtcgcc gtcgaacggc cggtcgcctt ggtcttcaac ggcatctcgc acgcggtgat
     3301 gctggccacg ccgctcgacc tggaggactt cgccctgggc ttcgcgctca gcgaggggct
     3361 gctggacagg gccgaggact gctacggcat cgaagcccac cccaccgccg atggcgtgga
     3421 agtgcacctg gacatcgcca gccgcgcctt cgctcgcctc aaggagcaac gccgtacgct
     3481 ggccggcgcg acgggctgcg ggctgtgcgg catggagcaa ctcggcatgc tggacctgca
     3541 tccgcagcgc gtaacggcca ggccggcgct gcaggccatt gccgcgccga cgatgctgaa
     3601 ggccgcggct gccctgccga gccatcaacc gctcaacacc agcaacggcg cgatgcacgc
     3661 ggccgcctgg gtctcgcccc agggcgagat ccagggcgtg ggcgaagacg tgggccggca
     3721 caatgcgctg gacaagctga tcggccgccg cctgcgcgcc aggcaggcgg gcggccagcc
     3781 ggccggcttg ggcgacggct tcgtgttgat gtccagccgc gccagttacg agctgctgcg
     3841 caagtgcgcg cgcctggaca t
//
LOCUS       DASUCY010000698         2318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1060246, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000698 DASUCY010000000
VERSION     DASUCY010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060246"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..169
                     /locus_tag="VFV25_13765"
     CDS             <1..169
                     /locus_tag="VFV25_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEY1394434.1"
                     /translation="IPVSLFTAIFALARTVGWIAQLNEMIGDPEYKIGRPRQLFVGSP
                     KREVKPIAQRS"
     gene            330..1322
                     /locus_tag="VFV25_13770"
     CDS             330..1322
                     /locus_tag="VFV25_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEY1394435.1"
                     /translation="MRFDLVTLNLVLAIAETHSITQGAKREHMALAAASKRLSDLETR
                     LGVALFERRARGVELTAAGRALVRHIHALNSSLHALESEVGEFAQGVRGHLRIAANAS
                     AISECLPPDLAAFSRAHPSVRISLEDQTSAEVQRSVAEGRADVGIFAPPLHEPRLATR
                     AYRSGRLAVIVPRRHVLAARASVKFEALLDFDIVGLHSGALVQELMLAQAAAVGKPLR
                     ARLQVRGFDAIAQLVEAGLGVAVMPAAVAERLSRLFSLQVLALDEPWAQRGYLIAVRE
                     QEVLPAVVGRFVDFLAHQADPGTEAGQDAAAEPINPARRAPAKTAKSAKIQPTA"
     gene            1330..>2318
                     /locus_tag="VFV25_13775"
     CDS             1330..>2318
                     /locus_tag="VFV25_13775"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011326219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEY1394436.1"
                     /translation="MKPARTLYDKIWDEHVVHTEEDGTTVLYIDRHLVHEVTSPQAFE
                     GLELAGRKVWRLSANLAVSDHNVPTTDRSHGIADPVSRLQVETLDKNCDRYGITQFKM
                     SDKRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTL
                     LARKARNMLVKVDGQLPRGCGAKDIVLAIIGRIGTAGGTGYTIEFGGSAIRTLSMEGR
                     MTVCNMAIEAGARAGLIAVDDTTIQYVKGRPYSPRGVEWEQAVAYWRALQSDAGAQWD
                     RVVELDASQIRPQVTWGTSPEMVLSIEDRVPDPEREKDAVKRNAMERALQYMAL"
BASE COUNT          384 a          810 c          773 g          351 t
ORIGIN      
        1 catcccggtg tcgctgttca cggccatctt cgcgctggcc cgcacggtcg gctggatcgc
       61 ccagctcaac gagatgatcg gcgaccccga gtacaagatc ggccgcccgc gccagctctt
      121 cgtcggctcg cccaagcgcg aggtcaagcc gatcgctcaa cgctcctgat tttgtagtcg
      181 atatccatat agaaccccgg ctcagccggg gtttttcttt aagccgcatc tgccttatcc
      241 ttcgtaatat gcgaaggctc ggttcatcat tgccagagag ttggctgcca gccttcgcgt
      301 atcgtgatga acctgggatc ccaaccggca tgcgcttcga cctcgtaacg ctcaatctcg
      361 tgctcgccat cgccgagacg cacagcatca cgcaaggcgc caagcgcgag cacatggccc
      421 tggcggcggc gagcaagcgc ctgtccgatc tggagacgcg cctgggcgtc gcgctgttcg
      481 agcggcgcgc ccgcggcgtc gaactcactg ccgccggccg cgccctggtg cgccacatcc
      541 atgcgctgaa ctcgtccctg catgcgctgg aaagcgaggt tggcgaattc gcgcagggcg
      601 tgcgcgggca tctgcgcatc gcggccaatg cctcggccat ctccgaatgc ctgccgccgg
      661 acctggcggc cttctcgcgc gcccacccct cggtgcgcat cagcctggaa gaccagacca
      721 gcgccgaggt gcagcgcagc gtggcggaag ggcgcgccga tgtcggcatc ttcgcgccgc
      781 cgctgcacga gccgcggctg gcgacgcgcg cctatcgcag cgggcgcctg gccgtcatcg
      841 tgccgcgccg ccacgtgttg gcggcgcgag cgagcgtgaa gttcgaggcg ctgctggact
      901 tcgacatcgt cggcctgcac agcggcgccc tggtgcagga gctgatgctg gcccaggccg
      961 ccgcagtggg caagccgctg cgcgcgcgcc tgcaggtgcg cggcttcgac gccatcgccc
     1021 agctggtcga ggccggcctc ggcgtggccg tgatgcccgc cgccgtggcc gagcgcctga
     1081 gccgcttgtt ctcgctgcag gtgctggccc tggacgagcc ctgggcgcag cgcggctatc
     1141 tgatcgcagt gcgcgaacag gaggtgctgc cggccgtggt cggccgcttc gtcgacttcc
     1201 ttgcgcacca ggccgatccc ggcaccgagg ccggccagga tgccgctgca gaacccatca
     1261 acccagcccg ccgcgctcct gccaaaacag cgaaaagcgc gaaaattcaa cccaccgcct
     1321 gaggccgcca tgaagcccgc acgcacgctt tacgacaaga tctgggacga gcatgtcgtc
     1381 cacacggaag aagacggcac gaccgtgctc tacatcgatc gtcacctggt gcacgaggtg
     1441 accagcccgc aggccttcga gggcctcgag ctggccggcc gcaaggtctg gcgactgtcg
     1501 gccaatctgg cggtgagcga ccacaacgtg ccgaccaccg accgctcgca tggcatcgcc
     1561 gacccggtgt cgcgcctgca ggtcgagacg ctggacaaga actgcgaccg ctatggcatc
     1621 acgcagttca agatgagcga caagcgccag ggcatcgtgc acgtgatagg cccagagcag
     1681 ggcgccacgc tgcccggcat gacggtggtc tgcggcgatt cgcatacctc cacgcatggc
     1741 gccttcggcg cgctggccca tggcatcggc accagcgaag tcgagcatgt gctggccacg
     1801 cagacgctcc tggccaggaa ggccaggaac atgctggtca aggtcgacgg gcaactgccc
     1861 cgcggctgcg gcgccaagga catcgtgctg gccatcatcg ggaggatcgg cacggccggc
     1921 ggcaccggct acacgatcga gttcggcggc agcgcgatcc gcaccctcag catggaaggg
     1981 cgcatgacgg tgtgcaacat ggcgatcgag gccggcgcgc gcgccgggct gatcgcggtg
     2041 gacgacacca cgatccagta cgtcaagggc cgcccctatt cgccgcgcgg cgtcgagtgg
     2101 gagcaggccg tcgcctattg gcgtgcgctg cagtccgacg ccggcgcgca atgggaccgc
     2161 gtggtcgagc tcgacgcctc gcagatccgc ccgcaggtca cctggggcac ctcgcccgag
     2221 atggtgctgt ccatcgaaga ccgcgtgccc gatcccgagc gcgagaagga tgcggtcaag
     2281 cgcaacgcga tggagcgtgc gctgcagtac atggcgct
//
LOCUS       DASUCY010000699         2063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1445755, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000699 DASUCY010000000
VERSION     DASUCY010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1445755"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..984
                     /gene="gpmA"
                     /locus_tag="VFV25_13780"
     CDS             238..984
                     /gene="gpmA"
                     /locus_tag="VFV25_13780"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEY1394437.1"
                     /translation="MYKLVLLRHGESTWNLENRFTGWTDVDLTTTGVAQAQQAGRLLK
                     EADYEFDVAYTSVLKRAIWTLWHTLDQMDRTWLPVVNDWRLNERHYGALQGLNKAETA
                     KRYGDQQVLVWRRSYDTPPPALEASDPRSERDDPRYARLDPAQIPLTECLKDTVARVL
                     PCWNEVLAPAVSSGQRLLIAAHGNSIRALVKYLDGISDSDIVGLNIPNGIPLVYELDA
                     ALKPIRHYYLGDAQAAAEAAAAVARQGLQG"
     gene            complement(1002..1706)
                     /locus_tag="VFV25_13785"
     CDS             complement(1002..1706)
                     /locus_tag="VFV25_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEY1394438.1"
                     /translation="MTAHLLHGIAIVVLRFSSLDQQARHALVRWWANKMLRVLGIGVH
                     GSGELHPGPVLLAANHVSWLDIVVIHAVCPQARFISKSEVRHWPLLRTLVDAAGTLYL
                     ERDRRRDALRVVHLMAEALGRGDTLVVFPEGTTGEGPQPLPFHANLLQAAVAVNAKVQ
                     PVALRFSDRKQAFSPAVAYVGDTSLLQSLWWVVCAEGLQAHLTLLPSQAAAGAERRAL
                     AAALRERIEAELKAVA"
     gene            complement(1747..>2063)
                     /locus_tag="VFV25_13790"
     CDS             complement(1747..>2063)
                     /locus_tag="VFV25_13790"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552753.1"
                     /note="Catalyzes the reversible hydrolysis of the amide
                     bond within dihydroorotate. This metabolic intermediate is
                     required for the biosynthesis of pyrimidine nucleotides;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEY1394439.1"
                     /translation="LALKWGEDSGAGLAKALAVVTSAPVRVLGEALGSLASSAGRLVE
                     GGVADVCVFDPEARWKAEPARLASQGKHTPFSFGATGMALPGRVMHTLVAGNVAYQRG
                     EA"
BASE COUNT          372 a          726 c          681 g          284 t
ORIGIN      
        1 gttggggctc agggcgcctg cgccgccgcg gcgcgagagg ctgggccaca aaagcaggcc
       61 gccggagacc aaggcggcca gaaccagata ccagttgtcg agaagaaatt tcagcatgag
      121 cccgtggcct cggcgcagcg acgatgcgct gttgaagaaa tcgggaatta tagaattcga
      181 tattttggcc cctgcctcgc cggggcttgc cttccatcag ctctcctgcc caagctcatg
      241 tacaagctcg tcctgctccg ccacggtgaa tcgacctgga acctggaaaa ccgtttcacc
      301 ggctggaccg atgtggatct cacgaccacc ggtgtcgcgc aggcccagca ggccggccgg
      361 ctgctcaagg aggcggacta cgaattcgat gtcgcctata cctcggtgct caagcgcgcg
      421 atctggacgc tgtggcacac gctggaccag atggaccgca cctggctgcc ggtggtcaac
      481 gactggcgcc tcaacgagcg ccactacggt gcgctgcagg gtctcaacaa ggccgagacc
      541 gccaagcgct acggcgacca gcaggtgctg gtgtggcgac gcagctatga cacgccgccg
      601 ccggcactgg aagcgagcga cccgcgcagc gagcgcgatg acccgcgcta cgccaggctc
      661 gacccggcgc agatcccgct caccgagtgc ctgaaggaca cggtggcgcg cgtgctgccc
      721 tgctggaacg aggtgctggc cccggccgtc agcagcgggc aacgcctcct gatcgccgcg
      781 catggcaaca gcatccgcgc gctggtgaag tatctggacg gcatctcgga cagcgacatc
      841 gtcgggctca acatccccaa cggcatcccg ctggtctacg aactcgatgc ggcgctcaag
      901 ccgatccggc actactacct gggcgacgcg caggctgcgg ccgaagccgc ggccgccgtg
      961 gcgcggcaag gccttcaggg ctgaaaaaac gggccgacgg ctcaggccac ggccttgagc
     1021 tcggcctcga tgcgctcacg tagcgccgcg gccaatgccc gcctttccgc gcccgccgcg
     1081 gcttgggacg gcagcagcgt cagatgggcc tgcaggccct cggcacaaac cacccaccac
     1141 agcgactgca ggaggctggt gtcgcccaca taggccacgg ccgggctgaa ggcctgcttg
     1201 cgatcggaga agcgaagggc cacgggctgg acttttgcat tcaccgcgac cgcggcctgc
     1261 agcagattgg catgaaaggg cagcggctgc ggcccttcgc ccgtcgtgcc ttcgggaaac
     1321 accaccagcg tgtcgccgcg gcccagcgcc tcggccatga gatggaccac gcgcagcgca
     1381 tcgcggcggc gatcacgctc cagatacagc gtgccggccg cgtcgacgag cgtgcgcagc
     1441 agcggccaat gccgcacctc ggacttggag atgaagcgcg cctgcgggca gacggcatgg
     1501 atcaccacga tgtcgagcca ggacacgtga ttggcggcca gtagcaccgg gcccggatgc
     1561 agttcgccgc tgccgtgaac gccgatgccg agcacgcgca gcatcttgtt ggcccaccac
     1621 cgcacaagcg catggcgggc ctgctgatcc aggctcgaga agcgcagcac cacgatggcg
     1681 ataccgtgca ataggtgcgc cgtcaccctc gacaagcgcc aagcagcacg cagccctcgc
     1741 attggctcag gcttcgccgc gctggtaggc cacgttgccg gccaccaggg tgtgcatcac
     1801 gcggcccggc aaggccatgc cggtggcgcc gaagctgaag ggtgtgtgct tgccctggct
     1861 cgccagccga gccggctcgg ccttccagcg cgcctcggga tcgaagacgc agacatccgc
     1921 gacaccgcct tcgacaaggc ggcccgcgct cgacgccagc gagcccagcg cttcgcccag
     1981 cacgcgcacc ggcgcgctgg tcacgacggc caatgccttg gcgaggccgg cgccgctgtc
     2041 ctcgccccac ttgagcgcca ggc
//
LOCUS       DASUCY010000700         4396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_529928, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000700 DASUCY010000000
VERSION     DASUCY010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529928"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /gene="pdeM"
                     /locus_tag="VFV25_13795"
     CDS             complement(<1..508)
                     /gene="pdeM"
                     /locus_tag="VFV25_13795"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     endonuclease PdeM"
                     /protein_id="HEY1394440.1"
                     /translation="MLRIELAGRPLVLLPDKAAYLPEVETLLVADAHIGKAVSFRKLG
                     VPVPRGTTSGNLQRITALVAQTQAKRVVFLGDFLHSERSHAASTLGALARWRATHSQL
                     ELTLVRGNHDERAGDPPPSARFEVVDEPLMLGGFALCHYPKPRVGHFVLAGHSHPCVS
                     LGGRAYDHL"
     gene            complement(528..1211)
                     /gene="slmA"
                     /locus_tag="VFV25_13800"
     CDS             complement(528..1211)
                     /gene="slmA"
                     /locus_tag="VFV25_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HEY1394441.1"
                     /translation="MPDETQAAVPEVAAAPAAEPAGGVVATSAEESQGRKRPKPGERR
                     IQILQTLAHMLEQPGAERITTAALAAKLDVSEAALYRHFASKAQMFEGLIDFIESSVF
                     TLVNQIAEREPHGAAQAQKVLTVLLQFGERNPGMTRVMVGDALVFENERLGARMNQFF
                     DRIESQFRQSLRAAAEAAGSQTPTVDAQALASVLTSFALGRLQRYARSGFKRLPTEHL
                     EAALRQLVH"
     gene            complement(1217..1906)
                     /locus_tag="VFV25_13805"
     CDS             complement(1217..1906)
                     /locus_tag="VFV25_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEY1394442.1"
                     /translation="MKKKPPHVWLFDLDNTLHDASYAVFGMIDVGMNDYIERELRVSR
                     EEADRLRRDYWRRYGATLLGLERHHGIRAAHFLEHAHRLPELEGGLRMNPRDRAALRA
                     LPGRKFVLTNAPAGYTKRVLTALDLAGCFEGVISIERMRMFGRLRPKPDARMFRMVLV
                     RLKLAAARCVLVEDTLAHQKAARGIGIASVWMQRYVVRNAHGPEVGARLRRRPTYCRA
                     RIRSLQKLRAL"
     gene            complement(1903..2574)
                     /locus_tag="VFV25_13810"
     CDS             complement(1903..2574)
                     /locus_tag="VFV25_13810"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HEY1394443.1"
                     /translation="MTIRAFFSWSGGKDSMLALHHALDAGVRVEALLAMHDESGERSR
                     SHGVPRALMQAQAEALGIPLVVRSAGWTDYERVFVDQLQRFAELGMTHGLFGDIDLQP
                     HRDWEERVCAAAGMTALLPLWQQDRRALADQVLALGYRARVVCVDARWLDASHCGVEY
                     DADFLARLPLGVDACGENGEFHTFVFDGPRFARPVAHGVAGIEELSMDVPAPGHYFVA
                     RLVGA"
     gene            complement(2645..3571)
                     /gene="argB"
                     /locus_tag="VFV25_13815"
     CDS             complement(2645..3571)
                     /gene="argB"
                     /locus_tag="VFV25_13815"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEY1394444.1"
                     /translation="MTTLSSTAAAAAAHDLSHIAPRDKAEILAQALPYIQRFHGKTLV
                     IKYGGNAMTDPALQQDFAEDIVLLKLVGMNPVVVHGGGPQIEDALSKLGKKGQFIQGM
                     RVTDAETMGVVEWVLAGEVQQDIVGLINAVGGKAVGLTGRDGGMIRARKLKMADRDDP
                     SKEHDLGQVGDIVSIDPGVVRALQDDQFIPVISPIGFGEENESYNINADVVAGKLAEV
                     LKAEKLMLLTNTPGVLDKSGKLLTNLSAREIDELFADGTISGGMLPKISSALDAAKSG
                     VNAVHIIDGRVPHAMLLEILSDQAYGTMIRAR"
     gene            complement(3708..>4396)
                     /locus_tag="VFV25_13820"
     CDS             complement(3708..>4396)
                     /locus_tag="VFV25_13820"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEY1394445.1"
                     /translation="ESLHQNCRFESAIGTVPPKGRLALSFDDGPDPVATPHILEVLGK
                     HHAPATFFMIGEQAQRHPDLVAQVRAHGEHLIGNHTWSHPNFHEISLPEQSEEIQKAQ
                     ALLTPNPEGRWYRYPYGNSTCDGNALLHLLDYRIVGWHIDSCDWAFDRNGEVDAQEAL
                     ECGVLPQYRSNYVEHVVSAARAHNGGIVLMHEIHPNTVRQLDEVLTALESAGFVFVRL
                     DEPAFEADMR"
BASE COUNT          703 a         1498 c         1450 g          745 t
ORIGIN      
        1 gcagatggtc ataggcccgc ccgcccaggc tcacgcaggg gtggctgtgg ccggccagca
       61 cgaagtggcc cacgcgcggc ttggggtagt ggcacaacgc gaaaccaccg agcatgagcg
      121 gctcgtcgac gacttcgaag cgcgccgaag gcggcggatc acccgcacgc tcgtcgtgat
      181 tgccgcgcac cagcgtcaat tccaattgag agtgggttgc gcgccagcgc gccagcgcac
      241 ccaaggtgga agccgcatgc gagcgctccg aatgcaggaa gtcgccgaga aagacgacgc
      301 gcttcgcctg cgtctgcgcc accagcgcgg tgatgcgctg cagattgccg ctcgtcgtgc
      361 cgcgcggcac cggcacgccc agcttgcgaa agctcactgc cttgccgata tgggcgtcgg
      421 ccaccagcag ggtttcgacc tcgggcaggt aggccgcctt gtcgggcagc agcacgagcg
      481 gccggccggc cagctcgatc ctgagcatgg cgctccccgc gatcctctca atgcacgagc
      541 tggcgcaatg cggcctccag atgctcggtg ggcagccgct tgaagcccga acgggcatag
      601 cgctgcaggc ggccgagcgc gaagctggtc agcaccgagg ccagggcttg ggcgtccacc
      661 gtgggcgtct gcgagcccgc ggcctcggcc gcggcacgca ggctttgccg gaactgcgat
      721 tcgatgcgat cgaagaactg gttcatgcgc gcgcccaggc gctcgttctc gaacaccagc
      781 gcgtcgccca ccatcacgcg cgtcatgccg gggttccttt cgccgaactg cagcagcacg
      841 gtcaacactt tctgcgcctg cgccgcgcca tggggctcgc gctcggcgat ctggttgacc
      901 agggtgaaga cgctgctctc gatgaagtcg atcaacccct cgaacatctg cgccttgctg
      961 gcaaaatggc ggtacagcgc cgcttcgctg acgtccagct tcgcggcaag cgcggcggtc
     1021 gtgatgcgct cggcgcccgg ctgctccagc atatgggcca gggtctgcag gatctggatg
     1081 cggcgctcgc ccggcttggg gcgcttgcga ccctggctct cttcggccga tgtggcgaca
     1141 acgccgccgg ccggctcggc agcaggagcg gcagccacct cgggcaccgc ggcttgggtt
     1201 tcgtctggca gggtgttcac agcgctcgta acttctgtag cgaacggatt ctcgcacggc
     1261 agtatgtggg cctgcggcgc aggcgtgcgc ccacctccgg cccatgtgcg ttgcgcacca
     1321 catagcgctg catccacaca ctggcgatgc cgatgccgcg cgccgccttc tggtgcgcca
     1381 acgtgtcttc gaccagcacg cagcgcgccg ccgcgagctt cagccgtacc agcaccatgc
     1441 ggaacatgcg cgcgtcgggc ttgggccgca gccggccgaa catgcgcatg cgttcgatgc
     1501 tgatcacgcc ttcgaagcag cctgcaaggt cgagcgccgt cagcacccgc ttcgtatagc
     1561 cggccggcgc attggtcagc acgaacttgc gcccgggcag cgcgcgcagc gccgcgcgat
     1621 cgcgcgggtt catgcgcaag cccccttcga gttccggcag ccggtgcgca tgttcgagga
     1681 agtgcgccgc gcggatgcca tggtgccgtt ccagccccag cagcgtggca ccgtagcggc
     1741 gccaatagtc tcgcctcaac cggtcagcct cttcgcgcga caccctgagc tcgcgctcga
     1801 tgtagtcgtt catgcccacg tcgatcatgc cgaagaccgc atacgaggca tcgtgcaggg
     1861 tgttgtcgag atcgaagagc cagacgtgcg gcggcttctt cttcatgcgc ccaccagtcg
     1921 cgccacgaag tagtgcccgg gggccggcac gtccatgctc agctcctcaa tgcccgcgac
     1981 gccatgcgcc accggccgcg cgaagcgcgg cccgtcgaag acaaaggtat gaaactcgcc
     2041 gttctcgccg caggcatcga cgcccagcgg caggcgcgcc aggaaatcag cgtcgtattc
     2101 gacgccgcaa tgactcgcgt ccagccagcg cgcgtcgacg cagacgacac gggcacgata
     2161 gcccagcgcc agcacctgat cggccagcgc ccgcctgtcc tgttgccaca agggcagcaa
     2221 ggccgtcatg cccgcggcgg cgcacactcg ctcctcccaa tcgcggtgcg gctgcaggtc
     2281 gatgtcgccg aagaggccat gcgtcatgcc gagctctgcg aagcgctgca gttgatcgac
     2341 gaagacgcgc tcgtagtcgg tccaccccgc gctgcgcaca accagcggta tccccagcgc
     2401 ttcggcctgg gcctgcatca gcgcgcgcgg cacgccgtgc gagcgcgagc gctcgccgct
     2461 ctcgtcatgc atcgccagca gcgcctcgac gcgtacgccg gcgtccagcg cgtggtgcag
     2521 ggccagcatc gaatccttgc cgccactcca ggagaagaag gctctgatcg tcatcgcatg
     2581 aagggcatag aaaggaggcc gcgcggcgcg gcctcggcgg cttcgggccg gctagaacag
     2641 ctgctcagcg cgcgcggatc atcgtgccgt aggcctgatc gctcaggatc tccagcagca
     2701 tcgcatgcgg cacgcggccg tcgatgatgt gcaccgcatt gacgccgctc ttggccgcat
     2761 ccagggccga cgagatcttg ggcagcatgc cgccggagat ggtgccgtct gcgaacagct
     2821 cgtcgatctc gcgcgcactc aggttcgtca acagcttgcc ggacttgtcg agcacgcccg
     2881 gcgtgttggt gagcagcatc aacttctcgg ccttcagcac ctcggccagc ttgccggcca
     2941 ccacgtcagc gttgatgttg tagctctcgt tctcctcgcc gaagccgatc gggctgatga
     3001 cggggatgaa ctggtcgtcc tgcaaggccc tcacaacgcc ggggtcgatc gagacgatgt
     3061 cgccgacctg gccgaggtcg tgttccttgc tcgggtcgtc gcggtcggcc atcttgagct
     3121 tgcgcgcgcg gatcatgccg ccgtcgcggc cggtcagccc cacggccttg ccgcccacag
     3181 cgttgatcag gcccacgatg tcctgctgca cctcgccggc cagcacccac tcgaccacgc
     3241 ccatggtctc tgcatcggtc acgcgcatgc cctggatgaa ttggcccttc ttgcccagct
     3301 tgctgagcgc gtcctcgatc tgcggcccgc cgccgtgcac gaccaccgga ttcataccca
     3361 ccagcttcag cagcacgatg tcctcggcaa agtcctgctg cagggccggg tcggtcatcg
     3421 cattgccgcc gtacttgatg accagcgtct tgccgtgaaa gcgctggata tagggcaggg
     3481 cctgggccag gatctcggcc ttgtcgcgcg gcgcgatgtg gctcagatca tgcgccgcag
     3541 cggcggcagc ggtagacgat agtgttgtca tgatggtctg gtgcccttcg cggaagccgt
     3601 gtcgccggca ttttatgggt ggcggcctgg agagggcctc gcacgcccct caccagttcg
     3661 accgtggcaa ggggcagccg cgcggcctat gtcgcccatt tagggcgtca tcgcatatcg
     3721 gcttcgaacg ccggctcgtc cagccgtaca aagacgaaac ccgccgactc gagcgcggtg
     3781 aggacttcgt cgagctgccg caccgtgttg gggtggatct cgtgcatcaa cacgatgccc
     3841 ccgttgtgcg cacgcgctgc cgaaaccaca tgttcgacat agttcgaacg gtactggggc
     3901 agcacgccgc actccagtgc ctcctgcgcg tcgacctctc cattgcgatc gaaggcccag
     3961 tcgcaggaat cgatgtgcca gccgacgatg cggtagtcca gcaggtgcaa cagggcattg
     4021 ccgtcgcagg tcgagttgcc gtaggggtag cgataccagc gcccctcggg attcggcgtg
     4081 agcagcgcct gtgccttttg gatttcttcg ctctgctcgg gcagggaaat ctcgtggaaa
     4141 ttgggatggc tccatgtgtg gttgccgatg aggtgctcgc cgtgggcgcg cacctgggcc
     4201 acgaggtcgg gatgccgctg cgcctgctcg ccgatcatga agaaggtcgc aggtgcgtgg
     4261 tgcttgccga ggacttccag tatgtggggc gtggcaacgg ggtccggccc gtcatcgaag
     4321 ctcagggcca gccggccctt gggcggaacc gtgccgatgg cggactcgaa gcggcagttc
     4381 tggtgcaggc tttcgg
//
LOCUS       DASUCY010000701         6014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1590305, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000701 DASUCY010000000
VERSION     DASUCY010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 6014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6014
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590305"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..1429
                     /locus_tag="VFV25_13825"
     CDS             116..1429
                     /locus_tag="VFV25_13825"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEY1394446.1"
                     /translation="MEITATVSSPDAPAGPARHPLTWVPSLYLAMGLPNVMVGVVAAI
                     IYKNLGVPNEDIALYTSQMYLPWVLKPLWAPLLEAHRTKRWWVIAMQFTMAASLGMVA
                     FCLPLEGFFRASLAFFWITGFASATQDTVADGVFMTTMPITEQARYAGLQGMCWNLGA
                     VIASGLLVSLTGWLHGRMGLDWAQCWMIVVGTAALAMAAFGLWHLRVLPPGGASSVRG
                     QGLAAGFAALRDAWLTFFAKPQIWMMLAVIFFYRFGEGFIEKFGPLFLLDPRGLGGLG
                     LDNQALGAINGTVGTIAFIAGAFLGGFLVARRTLPRSFFVLALVLNIPHLTYFYLSQT
                     LPESTLWIGAIVAAEKFGFGMGSVGHMLYMMQQVAPGPFKMTHYAMATGVMALTKWST
                     GTVSGWVWAAVGHDYRVFFALVLLASVPPVVLAWLAPFPHRERAA"
     gene            complement(1543..3249)
                     /locus_tag="VFV25_13830"
     CDS             complement(1543..3249)
                     /locus_tag="VFV25_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HEY1394447.1"
                     /translation="MKLPAGYAQAKGLQESPLGEIQLDQVGFLPRAAKWAAVPEAGSD
                     AFTVINAHTGQQVLRGHLGAAAFWEPAQASVRLADFSRLTMPGEYRLRVAGLPDSPRF
                     AVAPDVYAALNAASIKAFYFNRASMELQPEYAGPYARAAGHPDTRVLIHSSAAGPSRP
                     AGAVISSPKGWYDAGDYNKYVVNSGITVYSLLAAYEHFPEFFQRQTLNIPESGNGIPD
                     LLNEVLWNLDWLLTMQDPVDGGVYHKLTSKNFDGAVMPHEAAAERFVVQKTTAAALDF
                     AAVMATASRVFEGFEAQRPGLSARMLAAAKSAWGWAQGHPATVYRQPPDIHTGDYGDS
                     RLDDEFTWAAAELYITTREDAYYVAMKPRLAQISVPSWSEVNGLAWMSLAHHRHRLTA
                     AADQRLIVERVGGLAAQLAAVWQASPYRVGMQTADFVWGSNGVALNQAMMLLQGYRLN
                     GDRRELDAAQAALDYVLGRNALGMSMVTGFGEKSPLHPHHRPSLASGIAAPVPGFVVG
                     GPNPGQQDSANCPAAYPSKAPARSYLDHGCSYASNEVAINWNAPLVYVSAALQALGGA
                     IR"
     gene            complement(3341..4285)
                     /locus_tag="VFV25_13835"
     CDS             complement(3341..4285)
                     /locus_tag="VFV25_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEY1394448.1"
                     /translation="MPDARTNRFVRSHLKTRQLVLLVELGRHGSILHAAEAAHLTQPA
                     ASKLLSELEHALGVQLFERLPRGVAPTWYGEVMIRRAGAALAELDAGHQEVMELLSGL
                     SGRVAVGTVLTPSTSLLPEAVRRLKLGHPRLRVSISVDNSKPLIQQLRAGELDIVIGR
                     VLDSESAAELNFEAVTDEPHSIIVRAGHPLLERQDLRLQDLAQQSWILPPSGSILRDR
                     LTALFLSSGLDQPSEAVETLAMPATTSLLMGSDMIAALPPELVQPYLDAGLLKVLPFD
                     LGLRMDAYGIVTRKHHQLSPGAAAMLAVLREVTAGKRG"
     gene            complement(4310..5095)
                     /locus_tag="VFV25_13840"
     CDS             complement(4310..5095)
                     /locus_tag="VFV25_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEY1394449.1"
                     /translation="MTLRLQDKVAIVTGSTLGIGKAIARLFAAQGAFVVLNSHRDDEA
                     AAAMRAELGTESANGRAMFVQADVSDAASVQAMVERAVARFGRVDVLVNNAGMNVFAD
                     PLEMTAEQWQRCFAVDLDGAWNAIRAALPHMLAQGAGSIVNIASVHGHRIIPHCFPYP
                     VAKHALVGLSKALGIEYAARGIRVNSISPGLIMTEAIEAWLASLPDPQAERERQAALL
                     PCKRIGQPEEVAYTALLLASDEARFINATDILIDGGRSQLYHD"
     gene            complement(5100..>6014)
                     /locus_tag="VFV25_13845"
     CDS             complement(5100..>6014)
                     /locus_tag="VFV25_13845"
                     /inference="COORDINATES: protein motif:HMM:NF019365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HEY1394450.1"
                     /translation="DDVLKQARRAAALGLQIELTLHYSDFWTNSKTQRIPADWARQLD
                     ALPDETARMDRLCRFVNERTGEVLQALRSQGTPPQFVSLGNEIESGMLYPYGAATAEN
                     WPRLARLLQAGHAAVKAAVPEARVILHLDDAGNLEKYRHWFDQARAHGVDWDVIGASY
                     YPFWTRKTVEQVADFSRATTARYDRDLMIMEAGFNFAPTLPDGWPGQLSDNGPYPASM
                     SSPQGQRDFIDELFNGLKRAGRVLGVLYWDPIMIDCPGIGWALREGDSKPGANVVANT
                     TLFDFKGRALPVLDVWRDHAPAMKLLKT"
BASE COUNT          908 a         2010 c         2053 g         1043 t
ORIGIN      
        1 ttcgcgactt cgtctatcgg ggcgtcgcgt gaagggtcgg atggtcgccc ggccgactgc
       61 cgtgccgctt gcgcaatgac gcgcgaagcc cgagattcgt attcctccca tccccatgga
      121 aatcactgct actgtgtcct cgccggatgc tcctgccggg cccgcccgcc accctttgac
      181 ctgggtgcct tcgctctacc tcgcgatggg gctgcccaat gtgatggtcg gcgtggtcgc
      241 cgccatcatc tacaagaacc tcggcgtgcc gaacgaggac attgcgctct acacctcgca
      301 gatgtacctg ccctgggtat tgaagccgct ttgggcgccg ctgcttgagg cccatcgaac
      361 caagcgctgg tgggtcatcg cgatgcagtt cacgatggcc gcatcgctcg gcatggtggc
      421 cttctgcctg ccgctcgaag gcttcttccg cgcatcgctc gccttcttct ggatcaccgg
      481 cttcgcctcc gccacgcagg acaccgtggc cgacggcgtc ttcatgacga cgatgccgat
      541 caccgagcag gcgcgctacg cgggcttgca gggcatgtgc tggaacctgg gcgcggtcat
      601 cgcctccggc ctgctggtgt cgctcacagg gtggctgcac ggccgcatgg gcctggactg
      661 ggcgcagtgc tggatgatcg tcgtcggcac cgccgcgctc gcgatggccg cgttcggctt
      721 gtggcacctg cgcgtgttgc cgccgggcgg cgcgtcgagc gtgcgcggcc aggggctggc
      781 tgccggcttc gcggcgctgc gcgacgcctg gctcaccttc ttcgccaagc cgcagatctg
      841 gatgatgctg gcggtgatct tcttctaccg cttcggcgaa ggcttcatcg agaagttcgg
      901 cccgctgttc ctgctcgacc cacgcggcct gggcggcctg gggctggaca accaagcgct
      961 gggcgccatc aacggcaccg tgggcaccat cgccttcatc gccggcgcct tcctcggcgg
     1021 cttcctggtg gcgcggcgca cgctgccgcg ctcgttcttc gtgctggcgc tggtgctcaa
     1081 catcccgcac ctcacgtatt tctacctgag ccagacgctg ccggagagca ccttgtggat
     1141 cggcgccatc gtggcggccg agaagttcgg cttcggcatg ggttcggtcg gccacatgct
     1201 gtacatgatg cagcaggtcg cgcctgggcc gttcaagatg acccactacg ccatggccac
     1261 cggcgtgatg gcgctgacga aatggtccac cggcaccgtg agcggctggg tctgggcggc
     1321 cgtggggcac gactatcggg tgttcttcgc tctggtgttg ctggcctcgg taccgcccgt
     1381 ggtactggct tggctggcgc cgttcccgca ccgtgaacgg gcagcctgag gcgtagatgc
     1441 cgcgccgtcg gcgaggcgcc gcgaacgtcc agcggatccg atgccgatag gtctgtccct
     1501 ggatggttca gccgcaggat gggcgcggct gccattcaag agtcatcgaa tcgcaccgcc
     1561 gagcgcctgc aaggcggcgg acacatagac gagcggcgcg ttccagttga tcgccacctc
     1621 gttgctcgcg tagctgcagc cgtggtcgag gtaggagcgg gccggcgctt tcgacggata
     1681 ggccgccgga cagttcgcgc tgtcctgctg cccgggattg gggccgccga cgacgaagcc
     1741 gggcactggt gccgcgatgc cgcttgccag cgacgggcga tgatgcggat gcagcgggga
     1801 tttctcgccg aagccggtga ccatcgacat gcccagcgca ttgcgcccca gcacatagtc
     1861 cagcgcggcc tgtgcggcgt cgagttcgcg tcgatctccg ttcaggcggt agccctggag
     1921 gagcatcatg gcctggttca gggcgacgcc gttgctgccc cagacgaagt ccgccgtttg
     1981 catgccgacc cgatagggcg aggcctgcca gacagccgcc agttgcgctg ccaggccgcc
     2041 aacgcgttcg acgatcagtc tctggtccgc cgcagccgtg agccgatgcc gatggtgcgc
     2101 cagcgacatc caagccaagc cgttcacctc actccatgac ggcacgctga tctgcgcgag
     2161 ccgcggcttc attgcgacgt agtaagcgtc ctcgcgcgtg gtgatgtaga gctccgccgc
     2221 ggcccaggtg aactcgtcgt cgagtctgct gtcgccgtaa tcgccggtgt ggatgtccgg
     2281 gggttgccga tagaccgtcg ccggatgacc ctgcgcccag ccccatgccg acttcgcggc
     2341 tgccagcatg cgcgccgaca ggccggggcg ctgcgcttcg aagccctcga agacgcggct
     2401 ggccgtcgcc atcacggccg cgaagtccaa ggctgcggca gtggtcttct gcaccacgaa
     2461 gcgctcggcg gcggcttcat gaggcatgac cgcgccatcg aagtttttgc tggtgagctt
     2521 gtgatagacg ccgccgtcga ccgggtcctg catcgtgagc agccagtcca ggttccatag
     2581 cacctcgttc agcaggtcag ggatgccatt gccgctctcg gggatgttca gcgtctggcg
     2641 ctggaagaac tcggggaaat gctcgtaggc tgccagcagc gagtagacgg tgatgccgga
     2701 attgacaacg tacttgttgt agtcgccagc gtcgtaccag cccttggggc tggagatcac
     2761 cgcgcccgcc gggcgggacg ggccggcagc ggacgagtgg ataaggacgc gggtatccgg
     2821 atgccctgcc gcgcgcgcat aaggccctgc atattcgggc tgcagttcca tgcttgcgcg
     2881 attgaaatag aaggccttga tgctcgcggc gttgagggcc gcatagacat caggcgcgac
     2941 ggcgaagcgg ggcgaatcgg gcagcccagc cacccgcagc cggtattccc ccggcatggt
     3001 caaccgagag aagtcggcca gccgcaccga ggcctgtgcc ggctcccaga aggccgccgc
     3061 acccaagtgc ccgcgcaaga cctgttgccc ggtgtgagcg ttgatcaccg tgaacgcgtc
     3121 gctgcccgcc tccggcacgg ccgcccactt ggccgcccga ggcaggaagc ccacctgatc
     3181 gagctggatc tccccaagcg gcgactcctg caggcccttc gcctgggcat agcccgccgg
     3241 cagcttcatc cctacgggaa agggtgctcc cagcgtcaat gagacggcca agcaggccaa
     3301 gcgcaagctc ttcatcccga cccctggctt gcgttgaccg tcagccccgc ttcccggccg
     3361 tcacttcgcg cagcacggcc agcatcgctg ccgctcccgg cgaaagctga tggtgcttgc
     3421 gcgtgacgat gccataggca tccatgcgca ggcccaggtc gaaaggcagc accttgagca
     3481 ggcccgcgtc caggtagggc tggaccagtt cggggggcag cgccgcgatc atgtcgctgc
     3541 ccatcagcag gctggtggtc gctggcatgg cgagcgtttc gacggcctcg ctgggctggt
     3601 ccaggccgct ggagaggaag agcgccgtga gccggtcccg caggatgctg ccgctgggcg
     3661 gcaggatcca gctctgctgg gccagatcct ggagccgcag atcctgccgc tcgagcagcg
     3721 gatggccggc gcgcacgatg atgctgtgcg gctcgtcggt caccgcctcg aagttcagct
     3781 cggcggccga ttcgctgtcc agcacccggc cgatcacgat gtcgagttcg ccggctcgca
     3841 gctgctggat cagcggtttg ctgttgtcca ccgagatcga tacccgcagg cgcggatgcc
     3901 cgagcttgag ccggcggacg gcctcgggca gcaggctggt cgacggcgtg agcacggtgc
     3961 cgacggcgac gcggccgctc aggccagaca acagttccat cacctcctgg tggcctgcgt
     4021 cgagctcggc cagcgcagcg ccggcgcggc gaatcatgac ttcgccatac caggtgggcg
     4081 cgacgccgcg cggcaggcgc tcgaagagct gcacccccag cgcatgttcc agctcagaca
     4141 gcagcttgga ggccgccggc tgcgtgagat gcgccgcttc cgctgcgtgg aggatggagc
     4201 catggcggcc cagctccacc agcagcacca gctggcgggt cttcagatgg gatcggacaa
     4261 agcggttggt acgggcgtcg ggcatgcgcg tcttggcggc tgcttcgcgc tagtcgtgat
     4321 agagctgcga gcgtccgccg tcgatcagga tgtcggtggc attgatgaag cgagcttcat
     4381 cgctggccag caagagcgca gtgtaggcaa cctcctcggg ctgcccgatg cgcttgcagg
     4441 gcagcagcgc cgcctggcgc tcgcgctcgg cctgcggatc gggcagcgac gccagccacg
     4501 cctcgatggc ctcggtcatg atcaggcccg gcgagatgga attgacgcgg atgccgcgtg
     4561 ccgcgtattc gatgcccagc gccttgctca gtcccaccag cgcgtgcttg gccaccgggt
     4621 aggggaagca atgcggaatg atgcggtggc cgtggaccga cgcgatgttg acgatgctgc
     4681 ctgcaccttg cgccagcatg tggggcagtg cggcgcggat ggcgttccag gcgccgtcca
     4741 ggtcgacggc gaagcagcgc tgccattgct cggccgtcat ctccagcgga tcggcgaaga
     4801 cgttcatgcc tgcgttgttg acgagcacat cgacgcggcc gaagcgggca acggcgcgct
     4861 cgaccatggc ctggacggac gctgcgtccg acacgtcggc ctgcacgaac atggcgcgcc
     4921 cgtttgccga ctcggtaccg agctccgcgc gcatggccgc ggcggcctcg tcgtcgcgat
     4981 ggctgttcag caccacgaag gcgccttgcg ccgcgaacag ccgtgcaatc gccttgccga
     5041 tgccgagcgt ggagccggtg acgatcgcca ccttgtcttg aagtcgaagc gtcatgtcgt
     5101 caggttttca gcagcttcat cgcgggcgcg tgatcgcgcc agacgtcgag caccggcaat
     5161 gcccggccct tgaaatcgaa cagggtggtg ttggccacca cattggcgcc gggcttgctg
     5221 tcgccttcgc gcagcgccca gccgatgccg gggcagtcga tcatgatcgg gtcccagtac
     5281 agcacgccca gcacacggcc ggcgcgcttg aggccgttga acagctcgtc gatgaagtcc
     5341 cgctgcccct gcggcgaact catgctcgcc gggtagggcc cattgtcact gagctggccc
     5401 ggccagccgt cgggcagcgt gggcgcgaag ttgaagccgg cttccatgat catcaggtcg
     5461 cggtcgtagc gggccgtggt cgcgcggctg aagtccgcca cctgctccac cgtcttgcgg
     5521 gtccagaagg ggtagtagga cgcgccgatc acgtcccagt ccacgccgtg cgcgcgcgcc
     5581 tggtcgaacc agtggcggta tttctccagg ttgcctgcat cgtccaggtg caggatgacc
     5641 cgcgcctcgg gcacggccgc cttcacggcc gcgtgaccgg cctgaagcag ccgggccagg
     5701 cggggccagt tctccgcggt cgcggcgcca taggggtaaa gcatccccga ctcgatctcg
     5761 ttgcccagcg aaacgaactg cggcggcgtg ccctgcgagc gcagcgcttg cagcacctcg
     5821 ccggtgcgct cgttcacgaa ccggcaaagc cgatccatgc gtgcggtctc gtcaggcagg
     5881 gcatccagct gccgggccca gtccgcgggg atgcgctgcg tcttcgagtt ggtccagaaa
     5941 tcgctgtaat gcagggtcaa ctcgatctgc aggcccagcg ccgcggctcg acgcgcttgc
     6001 ttgagcacgt cgtc
//
LOCUS       DASUCY010000702         4231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_1349314, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000702 DASUCY010000000
VERSION     DASUCY010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1349314"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..658
                     /locus_tag="VFV25_13850"
     CDS             173..658
                     /locus_tag="VFV25_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HEY1394451.1"
                     /translation="MNKRTWLLAAAVLAFGLAGCATTPAPQSIADTIAGTPELSTLNK
                     LVNDAGMADTLRASGPYTLFAPSNDAFKAVPAETMNALASNKEALKAVLAYHVVQGKF
                     ASSDVTNSNQKTLNGASLALAKAGTFVTVEDAMVTTADITATNGVVHVVDSVLMPPKK
                     K"
     gene            745..1896
                     /locus_tag="VFV25_13855"
     CDS             745..1896
                     /locus_tag="VFV25_13855"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEY1394452.1"
                     /translation="MSGAPTQSPDLVRRALTLGLAASPLLLPKGALAAGSAAPTMRVA
                     QLLDMSAEQQDLSRDYSTGVRLGLAEFNQANGGRNIQITTLNTDGSPASLKEALDKLR
                     EDASICALLGTVGDHLAVESIAAAHGAKLSIAHVAPWMADSRHDADDEVFALFASREM
                     QIRHALKSLEVMGVSELGVIYTDAAEQKAIQDEVNAMAARLKLRIATYVPAAGEDLTA
                     LPARMGSKVPAILLFLGGTLELAQFTQALSRQNLQRYVVSLADVDVPTLMQVGTGKAV
                     PLILTQVVPNPQTSSLPVVRNYRAGLKRLFDESPSQVSLAGYLAARYAAQVLLRVEPS
                     ASRARVLAEFQKRPAADLGGFNISFAASQRRGSTFVTQTLLGPDGRLIG"
     gene            complement(1927..2655)
                     /locus_tag="VFV25_13860"
     CDS             complement(1927..2655)
                     /locus_tag="VFV25_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HEY1394453.1"
                     /translation="MDLPRKLAGEALGTMLLLTVVIGSGIMAERLAGGNVAIALLANT
                     LATVGGLYVLIEIFGPISGAHFNPAVSAAMAWRGELPKAALLPYVVAQLLGAMLGAWL
                     AHAMFDLTILQFSSKARAGAGQWVAEAVATAGLLLVILRAPVGRAAAMVAAYIGAAYW
                     FTASTSFANPAAAFGRMFSDSFAGIAPASAPGFVLAELVGAAIGLAIHNALEPRLHPA
                     GHPNAIEASGDADDDAPTAKTAPR"
     gene            complement(2658..3164)
                     /locus_tag="VFV25_13865"
     CDS             complement(2658..3164)
                     /locus_tag="VFV25_13865"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013209977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEY1394454.1"
                     /translation="MTTNVLILCTHNSARSVLSEGMLNHWARKLGKDVRAYSAGSAPS
                     GRINPFALEVLQNAGVDTAGYRSKSWDEFSQDGAPPLAIVITVCDSAAAEQCPAFFGG
                     TGGQPVKVHWGYPDPSNAEGGDEGKRRAFELTRQAIGYRMLQLVQLPLEAMSKAELQQ
                     ALAEIAGS"
     gene            complement(3176..3655)
                     /locus_tag="VFV25_13870"
     CDS             complement(3176..3655)
                     /locus_tag="VFV25_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003107243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HEY1394455.1"
                     /translation="MKRFHVHVHVEDLAKSVAFYSRLFAAEPARLESDYAKWMLDDPR
                     INFAISTRGGKPGVDHLGLQTDSAEELAELKARAQAADMAVLDEGETTCCYAHSEKHW
                     ITDPQGVAWEHFHTLENIPVFSEKAAPADAAGASSCCAPTTRGKPVGIAVKSASSCC"
     gene            complement(3669..3992)
                     /locus_tag="VFV25_13875"
     CDS             complement(3669..3992)
                     /locus_tag="VFV25_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEY1394456.1"
                     /translation="MEEKDIVRSLAALAQGARLRVFRALVVAGSRGMTPGVMAEGSGI
                     PPATLSFHLKELMNAGLVTQERLGRNLVYRASFEHMNALLGYLTENCCQGEACLPESS
                     TSCKC"
BASE COUNT          728 a         1448 c         1377 g          678 t
ORIGIN      
        1 gcgcactcta aaagcgcttg atgacaaatc cgtgacagtc tatgtgcttc acaaactgcc
       61 atttggctgt cacttaaaag tcactgtttc gtcataaacc ctgcctaagc tgcgcggcgc
      121 cggccgtctg agcctgcaaa agccctatcg ccccaagaac tcggagagct caatgaacaa
      181 acgcacttgg ctgctcgccg cggcggtcct ggccttcggc ctggccggct gcgccaccac
      241 gcccgcgcct caatccatcg ccgatacgat cgccggcacg cctgaattga gcacgctgaa
      301 caagctggtc aatgacgccg gcatggccga cacgctgcgc gcgagcggcc cctacacctt
      361 gtttgcgccc agcaacgatg ccttcaaggc cgtgcccgcc gagaccatga atgcgctcgc
      421 gagcaacaag gaagcgctca aggcggtgct cgcctaccac gtggtgcaag gcaagtttgc
      481 ctcgagcgac gtcaccaaca gcaaccagaa gaccctgaac ggcgcaagcc tggcgctggc
      541 caaggccggg accttcgtca ccgtggagga cgccatggtg acgaccgccg acatcaccgc
      601 taccaatggc gtggtacacg tggtcgacag cgtgctgatg ccgcccaaga agaaataaag
      661 aagaacgcct gaaaaaagcg gcgcccgggt gcgatacggc tcccgggcgc tacgtatttg
      721 atagcgatag cgaaagcctg tgccgtgagc ggagccccga cccaatcgcc cgatcttgta
      781 cgccgcgccc tgaccctggg cctggctgca agccccctgc tgctgcccaa gggcgccctg
      841 gccgccggca gcgccgcgcc gacgatgcgc gtcgcgcagc tgctcgacat gtcggccgag
      901 cagcaggatc tgtcgcgcga ctattcgacc ggcgtgcgcc tggggctggc cgagttcaac
      961 caggccaatg gcggccgcaa catccagatc accacgctca ataccgacgg cagtccggcc
     1021 tcgctgaagg aggccctgga caagctgcgc gaagacgcct cgatctgcgc cctgctgggc
     1081 accgtgggcg accacctggc ggtcgagagc atcgctgccg cacacggcgc caagctctcg
     1141 attgcacatg tcgcgccctg gatggccgat tcgcggcacg atgccgacga cgaggtcttc
     1201 gccctgttcg cctcgcgcga gatgcagatc cgccacgcgc tcaagagcct ggaggtgatg
     1261 ggcgtgagcg agctgggcgt gatttacacc gacgcggccg agcagaaggc gatacaggac
     1321 gaggtgaacg ccatggccgc gcgcctgaag ctgcgcatag cgacctatgt gcctgcagct
     1381 ggcgaagacc tcaccgccct gcccgcccgc atgggcagca aggtgccggc gatcctgctc
     1441 ttcctcggcg gcacgctgga actggcccag ttcacgcagg ccctcagccg gcagaacctg
     1501 cagcgctatg tggtcagcct ggccgacgtg gacgtgccga cgctgatgca ggtgggcacc
     1561 ggcaaggccg tgccgttgat cctcacccag gtcgtgccca atccgcagac cagcagcctg
     1621 cccgtggtgc gcaactaccg cgctgggctc aagaggctgt tcgacgagag cccttcccag
     1681 gtcagcctgg cgggctacct tgcggcgcgc tacgcggccc aggtcctgtt gcgcgtcgag
     1741 cccagcgcga gccgtgcgcg ggtgctggcc gagttccaga agcgccctgc cgcggatctc
     1801 ggcggtttca acatcagctt tgcagcatcc cagcggcgcg gcagcacctt cgtgacccag
     1861 accctgctcg ggccggacgg gcgcctgatc ggttgagcgg tcaggcgcag cgccatagca
     1921 gctgcgtcag cgaggagctg tcttcgcggt cggggcgtcg tcgtcggcat cgcccgaggc
     1981 ctcgatcgcg ttgggatggc cggccggatg cagccgaggt tcgagggcgt tgtgaattgc
     2041 cagtccaatg gctgcgccca ccagctcggc aagcacgaag cctggcgcac tggccggcgc
     2101 gatgccggcg aagctgtcgc tgaacatgcg cccgaaagcc gccgccggat tggcgaacga
     2161 ggtcgacgcg gtgaaccaat aagcggcgcc gatgtaggcc gcaaccatcg cagcggcgcg
     2221 gcctaccggc gcccgcagga tcacgagcag caggcccgcc gtggcgacgg cctcggcgac
     2281 ccattggccg gcccctgcgc gcgccttgct tgaaaactgc agaatcgtca ggtcgaacat
     2341 cgcatgcgcc agccaggcgc cgagcatggc gcccagcaac tgcgcgacga catagggcag
     2401 caacgccgcc ttcggtagct cgccgcgcca ggccatcgcg gcgctgactg ccggattgaa
     2461 atgcgcgccg ctgatcggac cgaagatctc gatcaggacg tacaggccgc ccaccgtggc
     2521 caaggtgttg gccagcagtg cgatcgcgac attgccgccg gccaggcgct cggccatgat
     2581 gcccgagccg atcacgacgg tcagcagcag catggtgccc agcgcttcgc ctgcaagctt
     2641 gcgtggcaga tccatcgtca gctcccggcg atctcggcca gcgcctgttg cagctcggcc
     2701 ttgctcatgg cttccagggg cagctgcacc agctgcagca tgcggtagcc gatggcctgg
     2761 cgcgtcagct cgaacgcgcg gcgcttgccc tcgtcgccgc cctcggcgtt cgacgggtcc
     2821 ggatagcccc aatgcacctt gaccggctgg ccgccggtcc cgccgaagaa ggcggggcac
     2881 tgctcggccg cggcgctgtc gcagaccgtg atgacgatgg ccagcggcgg cgcgccgtcc
     2941 tggctgaact cgtcccagct cttgctgcgg tagccggccg tgtccacgcc ggcgttctgc
     3001 agcacttcga gtgcgaacgg gttgatgcgg ccgctgggtg cactgccggc gctgtaggcg
     3061 cgcacgtcct tgccgagctt cctggcccag tggttgagca tgccttcgct gagcacgctg
     3121 cgtgcggagt tgtgggtgca gaggatgagg acgttggtgg tcatggcgat gactgtcagc
     3181 agcacgacga ggccgacttc acggcgatgc ccaccggctt gccgcgcgtc gtcggcgcgc
     3241 agcaggagga ggcaccggcg gcatcggccg gagctgcctt ctcgctgaac accgggatgt
     3301 tctccagcgt gtggaaatgc tcccaggcga caccctgcgg gtcggtgatc caatgctttt
     3361 cgctgtgcgc gtagcaacag gtggtctcgc cttcgtcgag caccgccatg tcggccgcct
     3421 gggcgcgtgc cttcagctcg gccagttcct cggcgctgtc cgtctgcaag ccgaggtggt
     3481 cgacgccggg cttgccgccg cgggtcgaga tcgcgaagtt gatgcgcggg tcgtcgagca
     3541 tccacttggc gtagtcgctc tcgaggcgag ccggctcggc ggcaaacagc ctggagtaga
     3601 aggccacgct tttcgcgagg tcctcgacat ggacatggac gtggaagcgc ttcatcgtgt
     3661 ttccttgctc agcatttgca ggaggtggag gattcgggca ggcaagcctc gccctggcag
     3721 cagttttcgg tgagatagcc caacagcgcg ttcatatgct cgaacgacgc ccgatagacg
     3781 agattgcggc ccagccgctc ctgcgtcacc aggccggcat tcatcagctc cttgaggtga
     3841 aaggacagcg tcgccggcgg gatgccagag ccctcggcca tcacgcctgg ggtcataccg
     3901 cgcgagccgg ccacgaccag tgcccggaac acgcgcaagc gggccccttg cgccaatgca
     3961 gccagggagc ggacgatgtc tttctcttcc atgtttccat gctaatggaa taacagaatg
     4021 caagcaattg ccgctcgcct gtgtcagcga tccgatagca accatccagc tcgctgcgtg
     4081 gccggccccg aggggcgccc cgctgcgcgc cctactaaac tcccgccttc cggcctaccc
     4141 ggccagcaac ccgccccaca gggcgctgcc tttccatgtt cgataccctc gaatccaccg
     4201 cgcgcaaggc tgcaccggcc ggcgtgcgcc a
//
LOCUS       DASUCY010000703         3927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Methylibium sp. isolate SMAG_U1309 k141_915599, whole
            genome shotgun sequence.
ACCESSION   DASUCY010000703 DASUCY010000000
VERSION     DASUCY010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961465
            Sequence Read Archive: SRR25158177
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methylibium sp. (soil metagenome)
  ORGANISM  Methylibium sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Sphaerotilaceae; Methylibium.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 05:57:13
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,778
            CDSs (total)                      :: 2,758
            Genes (coding)                    :: 2,736
            CDSs (with protein)               :: 2,736
            Genes (RNA)                       :: 20
            tRNAs                             :: 19
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 22
            CDSs (without protein)            :: 22
            Pseudo Genes (ambiguous residues) :: 0 of 22
            Pseudo Genes (frameshifted)       :: 3 of 22
            Pseudo Genes (incomplete)         :: 14 of 22
            Pseudo Genes (internal stop)      :: 5 of 22
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Methylibium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915599"
                     /isolate="SMAG_U1309"
                     /isolation_source="soil"
                     /db_xref="taxon:2067992"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.05861111 N 115.0369444 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1381
                     /locus_tag="VFV25_13880"
     CDS             <1..1381
                     /locus_tag="VFV25_13880"
                     /inference="COORDINATES: protein motif:HMM:NF020110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HEY1394457.1"
                     /translation="RARWLARDLQLRLMLPLLAIVAMTAGIGAYTAQRLTDRIFDRWL
                     LDAAQPLAAQVRFVDHRAELDLPPAAEAVLAYDEIDHVYFSVAQEGRHLAGQPGLPLQ
                     GQREAVYDNGRAYDASFGGQPVRVAAVQVNDGAGGKALALVAETTLKRRHAQQEILLM
                     LLPMGGLLLATAAAIGIAVRRTIRPLEAIAARWEQRSHASLELISADELPRELTPFAT
                     ALNGLLTRIRAMLAREQQFATTAAHQLRTPLAGLQLGLTRAAEAPDLAGTRAVLQELG
                     ETTQRTARLVQQLLALGRLDPEARGDLAFAPTDLVALAQDVGATYVDLALHRSISLEL
                     SPPAAPVIANVHPELFSEALGNLLDNALRYTPAGGRVLIEFLNDPPGLRVSDSGPGVP
                     EDERETVFERFARGRLATGDGSGLGLAIVRDIAALHGASVSLERSAQLGGASVVLRFA
                     PRQPKPAEA"
     gene            complement(1362..2744)
                     /locus_tag="VFV25_13885"
     CDS             complement(1362..2744)
                     /locus_tag="VFV25_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEY1394458.1"
                     /translation="MQAEHFDAIVIGAGQAGPALAARLDQAGRKTAFIERQLMGGTCV
                     NTGCIPTKTLVASARAAHVARRGAEYGFSAGEVRVDMRAVKARKDGVVGKSVAGLTGW
                     VEGMKNVSLIRGHAKFTAPHTIAVNDRLLQADQVFINVGGRAVVPDLPGVQDVPYLTN
                     TTMMGIDFLPEHLVIVGGSYIGLEFAQMYRRFGAEVTVVERAPKLLPREDDDVAAEIR
                     AILEREGIELRTNAECMELHRKGDRIEIGLSCRQGPPVAEGSHLLLAVGRVPNTHDLG
                     LDAAGVETDARGYIKVDDECRTSVEGVYALGDANGRGAFTHTSYNDYEIVAANLLDGE
                     QRRISDRIPAYALFIDPPLGRVGMNEGEARASGKRVLVGKMPMTRVGRAREAGETQGF
                     MKVLVDADTQLLLGASLLGLNADEIVHSLLDIMYARQPYTTIARAMHIHPTVTELVPT
                     LLQSLKPLQA"
     gene            complement(2797..>3927)
                     /locus_tag="VFV25_13890"
     CDS             complement(2797..>3927)
                     /locus_tag="VFV25_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEY1394459.1"
                     /translation="LAWPDPVALAKAGALLAQAQRPFVIAGGSGWTAESAASLQRFAE
                     SWQLPVGCAFRFQDCFDNRHSLYAGDVGIGINPKLAACVREADLVLAIGARLGEMTTG
                     GYTLLEVPRPKQKLIHIHAGPEELGRVYAADVLIEASMARAALALETLAVPAQLPWVE
                     WTAAAHADYEANHIATPVSPLDMAEVVKTISRLAPDAVYTNGAGNYSGWLHRYHRYPG
                     LQHHGRTQLAPTSGAMGYGVPAAVAASLLYPQRTVINIAGDGDFLMTGQELATATGYK
                     AGRLISIVVDNGTYGTIRMHQEREYPGRVSGSDLFNPDFAALALAYGWRAAKVERTEQ
                     FEPAFAAALESARPSLLHLKLDADVSTSRTSLTAIREAARRL"
BASE COUNT          550 a         1397 c         1354 g          626 t
ORIGIN      
        1 gcgcgcccgc tggctggcgc gcgatctgca actgcgactg atgctgccgc tgctggccat
       61 cgtcgcgatg accgccggca tcggtgccta cacggcgcag cgcctgaccg atcgcatctt
      121 cgaccgctgg ctgctcgacg cggcgcagcc gctcgcggcg caggtgcgtt tcgtcgatca
      181 ccgcgccgag ctcgatctgc cgcccgcggc cgaagcggtg ctggcctatg acgagatcga
      241 ccatgtctat ttcagcgtcg cgcaggaggg ccgccacctg gccggccagc ccggcctgcc
      301 gctgcagggc cagcgcgagg cggtctacga caatgggcgc gcctacgacg ccagcttcgg
      361 cggccagccg gtgcgggtgg ctgccgtgca ggtcaatgac ggggccggcg gcaaggcact
      421 ggcgctggtg gccgagacca cgctcaagcg ccgtcatgcg cagcaggaga tcctgctgat
      481 gctgctgccc atgggcggcc tgctgctcgc taccgcggca gccatcggca tcgccgtgcg
      541 gcgcacgatc aggccgctcg aggccatcgc cgcgcgctgg gagcagcgct cccacgcctc
      601 gctggaactc atcagcgccg acgagctgcc gcgcgagctg acgcctttcg ccaccgccct
      661 caacggcctg ctcacgcgca tacgcgcgat gctggcgcgc gagcagcagt tcgccaccac
      721 ggccgcccac cagctgcgca caccgctggc cggcctgcag ctgggcctca cgcgcgccgc
      781 cgaggcgccg gatctggccg gcacgcgcgc tgtgctacag gagctgggcg agaccacaca
      841 gcgcaccgcg cggctggtgc agcagttgct cgcgctcggc cgcctcgatc ccgaggcgcg
      901 cggcgacctg gccttcgcgc ccaccgacct ggtcgcgctg gcccaggacg tgggcgccac
      961 ctatgtcgac ctcgcgttgc accgttccat ctccctggag ctgagcccgc cggccgcgcc
     1021 ggtaatcgcc aatgtccacc ccgagctgtt cagcgaggcc ctgggcaatc tgctggacaa
     1081 cgcactgcgc tacacgccag cgggcggccg cgtgctgatc gagttcctga acgatccgcc
     1141 ggggctgcgc gtgtcggatt ccggccccgg cgtgcccgag gacgagcgcg agaccgtatt
     1201 cgagcgcttc gcgcgcggcc gcctcgcaac gggcgacggc agcggcctgg gcctggccat
     1261 cgtgcgcgac atcgccgcgc tgcacggcgc cagcgtcagc ctggagcgca gcgcccagct
     1321 gggcggggcc agcgtggtgt tgcgcttcgc accgcgccag cctaagcctg cagaggcttg
     1381 agactttgca gcagcgtcgg caccagctcg gtgaccgtgg gatgaatgtg catcgcgcgc
     1441 gcgatcgtcg tatagggctg gcgcgcatac atgatgtcca acagcgaatg cacgatctcg
     1501 tcggcgttca ggccgagcag ggaagcgccc agcagcaact gggtgtcggc gtccaccagc
     1561 accttcatga atccctgcgt ctcgccggcc tcgcgcgcgc ggccgacccg cgtcatcggc
     1621 atcttgccga ccagcacgcg cttgccgctc gcccttgctt cgccctcgtt catgccgaca
     1681 cgtcccagcg gcgggtcgat gaagagcgca taggccggga tgcggtccga gatgcggcgc
     1741 tgctcgccgt ccaggagatt ggcggcgacg atctcatagt cgttgtagga ggtgtgcgtg
     1801 aaggcgccgc ggccgttggc gtcgcccagc gcatagacgc cctcgacgct ggtgcggcat
     1861 tcgtcgtcga ccttgatgta gccgcgcgca tcggtctcga cgccggctgc gtctagcccc
     1921 aggtcgtgcg tgttcggcac gcgcccgact gccagcagca gatggctgcc ctcggccacc
     1981 ggcgggccct gccggcaaga caggccgatc tcgatgcgat cgcccttcct gtgcagctcc
     2041 atgcattcgg cattggtgcg cagctcgatg ccctcgcgct cgaggatggc gcggatttcc
     2101 gcggccacgt cgtcgtcctc gcgcggcagc agcttgggtg cccgctcgac caccgtcacc
     2161 tcggcgccga agcggcggta catctgcgcg aactccaacc cgatatagct gccgccgacg
     2221 atgaccaggt gctcgggcag gaagtcgatg cccatcatcg tcgtgttggt gaggtaaggc
     2281 acatcctgga cgcccggcag gtccggcacc accgcgcggc caccgacatt gatgaagacc
     2341 tggtcggcct gcagcaggcg atcgttcacg gcgatcgtgt gcggcgcggt gaactttgcg
     2401 tggccgcgta tcaggctcac gttcttcatg ccctcgaccc agccggtcag gccggccacc
     2461 gacttcccaa ccacgccgtc cttgcgcgcc ttgaccgcgc gcatgtcgac gcgcacctcg
     2521 ccggcgctga agccatactc cgcgccgcgc cgggccacgt gtgccgcgcg tgcgcttgcc
     2581 acaagcgtct tggtcgggat gcagcccgtg ttgacgcagg taccgcccat cagttgccgc
     2641 tcgatgaagg cggtcttgcg gccggcctgg tcgagtcgcg cggcaagcgc cgggccggcc
     2701 tggccggcac cgatgacgat ggcgtcgaag tgttcagctt gcatgggcga gctcccgaag
     2761 ctttggccaa gcatagtcgc tgctgggcct tctgccttac agccggcgtg ccgcctcgcg
     2821 tattgccgtc aggctggtgc ggctcgtgct cacgtcggcg tcgagcttca ggtgcagcaa
     2881 gctgggccga gcgctttcca gcgcggccgc aaaggccggc tcgaactgct cggtgcgctc
     2941 caccttcgcc gcccgccagc cataggccag cgccagtgcg gcgaaatcag gattgaacag
     3001 atcgctgccg ctgacgcggc ccgggtactc acgctcctgg tgcatgcgta tcgtgccgta
     3061 ggtgccgttg tcgaccacga tgctgatcag ccggccggcc ttgtagccgg ttgcggtggc
     3121 gagttcctgc ccggtcatca ggaagtcgcc gtcgcccgcg atgttgatca ccgtgcgctg
     3181 cggatacagc aggctcgccg ccacggctgc cggcacaccg tagcccatcg cgccgctggt
     3241 gggtgcgagc tgggtgcggc cgtggtgctg caggccggga tagcggtggt agcgatgcag
     3301 ccagccgctg tagttgccgg caccgttggt gtagaccgcg tcgggcgcga ggcggctgat
     3361 ggtcttgacc acctcagcca tgtccagcgg gctcaccggc gtggcgatgt gattggcttc
     3421 gtagtccgca tgggcggccg cggtccactc gacccaaggc aactgcgccg ggacggccag
     3481 cgtctccagc gccagggcgg cgcgggccat gctggcttcg atcagcacat cggcggcata
     3541 gacgcggccc agttcctccg ggccggcgtg gatatggatg agcttctgct tcggccgcgg
     3601 cacctcgagc agcgtgtagc cgccggtggt catctcgccc aggcgcgcgc cgatggcgag
     3661 cacgaggtcg gcctccctga cgcacgccgc gagcttgggg ttgatgccta tgcctacgtc
     3721 gccagcatag agcgaatgcc ggttgtcgaa gcagtcctgg aagcggaagg cgcaacccac
     3781 gggcagctgc cagctttcgg cgaagcgctg caggctggcc gcgctctcgg cggtccagcc
     3841 gctgccgcca gcgatcacga aggggcgctg ggcctgggcc agcagcgcgc cggccttcgc
     3901 cagcgcaacc ggatccggcc aggccag
//
